LOCUS       DAVKAO010000001        96130 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKAO010000001 DAVKAO010000000
VERSION     DAVKAO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 96130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.001"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            32..3049
                     /locus_tag="VWX16_000001"
     CDS             32..3049
                     /locus_tag="VWX16_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEU9157453.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSSSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMNVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINSNGNLEFELHF"
     gene            3064..3792
                     /locus_tag="VWX16_000002"
     CDS             3064..3792
                     /locus_tag="VWX16_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU9157454.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRSVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            3805..5571
                     /locus_tag="VWX16_000003"
     CDS             3805..5571
                     /locus_tag="VWX16_000003"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9157455.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNRAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLADLGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQAIRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            5717..6868
                     /locus_tag="VWX16_000004"
     CDS             5717..6868
                     /locus_tag="VWX16_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9157456.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            7013..7897
                     /locus_tag="VWX16_000005"
     CDS             7013..7897
                     /locus_tag="VWX16_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9157457.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(8015..8092)
                     /locus_tag="VWX16_000006"
                     /pseudo
     CDS             complement(8015..8092)
                     /locus_tag="VWX16_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            8296..8547
                     /locus_tag="VWX16_000007"
     CDS             8296..8547
                     /locus_tag="VWX16_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9157458.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            8535..8825
                     /locus_tag="VWX16_000008"
     CDS             8535..8825
                     /locus_tag="VWX16_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157459.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEDKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(9229..10560)
                     /locus_tag="VWX16_000009"
     CDS             complement(9229..10560)
                     /locus_tag="VWX16_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9157460.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIAYEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLASLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(10582..12093)
                     /locus_tag="VWX16_000010"
     CDS             complement(10582..12093)
                     /locus_tag="VWX16_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9157461.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            complement(12304..13251)
                     /gene="arcC"
                     /locus_tag="VWX16_000011"
     CDS             complement(12304..13251)
                     /gene="arcC"
                     /locus_tag="VWX16_000011"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9157462.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            complement(13416..14432)
                     /gene="argF"
                     /locus_tag="VWX16_000012"
     CDS             complement(13416..14432)
                     /gene="argF"
                     /locus_tag="VWX16_000012"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9157463.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            complement(14490..15719)
                     /gene="arcA"
                     /locus_tag="VWX16_000013"
     CDS             complement(14490..15719)
                     /gene="arcA"
                     /locus_tag="VWX16_000013"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9157464.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            16474..18153
                     /locus_tag="VWX16_000014"
     CDS             16474..18153
                     /locus_tag="VWX16_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9157465.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGVQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLIKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(18193..20277)
                     /locus_tag="VWX16_000015"
     CDS             complement(18193..20277)
                     /locus_tag="VWX16_000015"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9157466.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            20449..21729
                     /locus_tag="VWX16_000016"
     CDS             20449..21729
                     /locus_tag="VWX16_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9157467.1"
                     /translation="MVYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     INSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            21820..24465
                     /locus_tag="VWX16_000017"
     CDS             21820..24465
                     /locus_tag="VWX16_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9157468.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTARYAKILLKHELTVPVSADEKLEEEQKGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGI
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLDPQEIRTEFIKKEEI"
     gene            24549..25418
                     /locus_tag="VWX16_000018"
     CDS             24549..25418
                     /locus_tag="VWX16_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9157469.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHSELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            25412..27292
                     /locus_tag="VWX16_000019"
     CDS             25412..27292
                     /locus_tag="VWX16_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9157470.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAGTIADIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RHAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(27714..27863)
                     /gene="rpmG"
                     /locus_tag="VWX16_000020"
     CDS             complement(27714..27863)
                     /gene="rpmG"
                     /locus_tag="VWX16_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9157471.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(27879..28061)
                     /gene="rpmF"
                     /locus_tag="VWX16_000021"
     CDS             complement(27879..28061)
                     /gene="rpmF"
                     /locus_tag="VWX16_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9157472.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            complement(28213..28413)
                     /locus_tag="VWX16_000022"
     CDS             complement(28213..28413)
                     /locus_tag="VWX16_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9157473.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            complement(28416..29423)
                     /locus_tag="VWX16_000023"
     CDS             complement(28416..29423)
                     /locus_tag="VWX16_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9157474.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTMKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            29614..31644
                     /locus_tag="VWX16_000024"
     CDS             29614..31644
                     /locus_tag="VWX16_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9157475.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKVSSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
     gene            31648..31932
                     /locus_tag="VWX16_000025"
     CDS             31648..31932
                     /locus_tag="VWX16_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9157476.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKICEEKGLI"
     gene            31945..33291
                     /locus_tag="VWX16_000026"
     CDS             31945..33291
                     /locus_tag="VWX16_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9157477.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            33295..34152
                     /locus_tag="VWX16_000027"
     CDS             33295..34152
                     /locus_tag="VWX16_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9157478.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            34149..35081
                     /locus_tag="VWX16_000028"
     CDS             34149..35081
                     /locus_tag="VWX16_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9157479.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            35314..37017
                     /gene="ilvD"
                     /locus_tag="VWX16_000029"
     CDS             35314..37017
                     /gene="ilvD"
                     /locus_tag="VWX16_000029"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9157480.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(37133..37213)
                     /locus_tag="VWX16_000030"
                     /pseudo
     CDS             complement(37133..37213)
                     /locus_tag="VWX16_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(37271..37612)
                     /locus_tag="VWX16_000031"
     CDS             complement(37271..37612)
                     /locus_tag="VWX16_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9157481.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            37736..38587
                     /gene="rgg"
                     /locus_tag="VWX16_000032"
     CDS             37736..38587
                     /gene="rgg"
                     /locus_tag="VWX16_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9157482.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSAFLSVSLFTRYVREMVRKSDFLMEMSGNRNFFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            38780..40027
                     /locus_tag="VWX16_000033"
     CDS             38780..40027
                     /locus_tag="VWX16_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9157483.1"
                     /translation="MKLLFRNPAYRILTLSCFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            40105..41394
                     /locus_tag="VWX16_000034"
     CDS             40105..41394
                     /locus_tag="VWX16_000034"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9157484.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVSQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            41391..41468
                     /locus_tag="VWX16_000035"
                     /pseudo
     CDS             41391..41468
                     /locus_tag="VWX16_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            41735..42049
                     /locus_tag="VWX16_000036"
     CDS             41735..42049
                     /locus_tag="VWX16_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157485.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            42059..42265
                     /locus_tag="VWX16_000037"
     CDS             42059..42265
                     /locus_tag="VWX16_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9157486.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            42265..42462
                     /locus_tag="VWX16_000038"
     CDS             42265..42462
                     /locus_tag="VWX16_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157487.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            42465..43199
                     /locus_tag="VWX16_000039"
     CDS             42465..43199
                     /locus_tag="VWX16_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9157488.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            43211..43852
                     /locus_tag="VWX16_000040"
     CDS             43211..43852
                     /locus_tag="VWX16_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9157489.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            43870..44100
                     /locus_tag="VWX16_000041"
     CDS             43870..44100
                     /locus_tag="VWX16_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157490.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            44136..44216
                     /locus_tag="VWX16_000042"
                     /pseudo
     CDS             44136..44216
                     /locus_tag="VWX16_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            44452..46215
                     /gene="aspS"
                     /locus_tag="VWX16_000043"
     CDS             44452..46215
                     /gene="aspS"
                     /locus_tag="VWX16_000043"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9157491.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            46193..47134
                     /locus_tag="VWX16_000044"
     CDS             46193..47134
                     /locus_tag="VWX16_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9157492.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(47254..47334)
                     /locus_tag="VWX16_000045"
                     /pseudo
     CDS             complement(47254..47334)
                     /locus_tag="VWX16_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(47470..48456)
                     /locus_tag="VWX16_000046"
     CDS             complement(47470..48456)
                     /locus_tag="VWX16_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9157493.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(48466..49266)
                     /locus_tag="VWX16_000047"
     CDS             complement(48466..49266)
                     /locus_tag="VWX16_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9157494.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSIVIG
                     PGQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(49527..50369)
                     /locus_tag="VWX16_000048"
     CDS             complement(49527..50369)
                     /locus_tag="VWX16_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9157495.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(50371..51663)
                     /locus_tag="VWX16_000049"
     CDS             complement(50371..51663)
                     /locus_tag="VWX16_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9157496.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLSVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(51774..53045)
                     /locus_tag="VWX16_000050"
     CDS             complement(51774..53045)
                     /locus_tag="VWX16_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9157497.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            53595..55112
                     /gene="malQ"
                     /locus_tag="VWX16_000051"
     CDS             53595..55112
                     /gene="malQ"
                     /locus_tag="VWX16_000051"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9157498.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEDL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            55138..57396
                     /gene="glgP"
                     /locus_tag="VWX16_000052"
     CDS             55138..57396
                     /gene="glgP"
                     /locus_tag="VWX16_000052"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9157499.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(58440..59012)
                     /locus_tag="VWX16_000053"
     CDS             complement(58440..59012)
                     /locus_tag="VWX16_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157500.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLVSWFVNDVALE
                     FAMNLLSSGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(59811..60659)
                     /locus_tag="VWX16_000054"
     CDS             complement(59811..60659)
                     /locus_tag="VWX16_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9157501.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSKLTEITIEAEI
                     LVGKAF"
     gene            60811..61089
                     /locus_tag="VWX16_000055"
     CDS             60811..61089
                     /locus_tag="VWX16_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157502.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            61091..63154
                     /locus_tag="VWX16_000056"
     CDS             61091..63154
                     /locus_tag="VWX16_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9157503.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRICVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(63232..64488)
                     /locus_tag="VWX16_000057"
     CDS             complement(63232..64488)
                     /locus_tag="VWX16_000057"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9157504.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            64630..67095
                     /locus_tag="VWX16_000058"
     CDS             64630..67095
                     /locus_tag="VWX16_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9157505.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKAGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            67160..68071
                     /locus_tag="VWX16_000059"
     CDS             67160..68071
                     /locus_tag="VWX16_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9157506.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            68301..68999
                     /gene="dapD"
                     /locus_tag="VWX16_000060"
     CDS             68301..68999
                     /gene="dapD"
                     /locus_tag="VWX16_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9157507.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            69067..70197
                     /locus_tag="VWX16_000061"
     CDS             69067..70197
                     /locus_tag="VWX16_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9157508.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEAVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            70209..70748
                     /locus_tag="VWX16_000062"
     CDS             70209..70748
                     /locus_tag="VWX16_000062"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9157509.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKRVLTPKTYPKGRMDFVVYDPQQLVKTSFGLLEPQ
                     GDLEVVDVSKIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            70732..71409
                     /locus_tag="VWX16_000063"
     CDS             70732..71409
                     /locus_tag="VWX16_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9157510.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(71476..72375)
                     /gene="galU"
                     /locus_tag="VWX16_000064"
     CDS             complement(71476..72375)
                     /gene="galU"
                     /locus_tag="VWX16_000064"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9157511.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMNDYEKTHAST
                     IAVMPVPHEDVSSYGVIAPQGEGSNGLYSVETFVEKPAPEETPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(72397..73413)
                     /locus_tag="VWX16_000065"
     CDS             complement(72397..73413)
                     /locus_tag="VWX16_000065"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9157512.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRSAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            73625..74006
                     /locus_tag="VWX16_000066"
                     /pseudo
     CDS             73625..74006
                     /locus_tag="VWX16_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(74179..74604)
                     /locus_tag="VWX16_000067"
     CDS             complement(74179..74604)
                     /locus_tag="VWX16_000067"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9157513.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEIRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(74672..75322)
                     /gene="phoU"
                     /locus_tag="VWX16_000068"
     CDS             complement(74672..75322)
                     /gene="phoU"
                     /locus_tag="VWX16_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9157514.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAKL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPGEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(75337..76089)
                     /gene="pstB"
                     /locus_tag="VWX16_000069"
     CDS             complement(75337..76089)
                     /gene="pstB"
                     /locus_tag="VWX16_000069"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9157515.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(76091..76906)
                     /gene="pstA"
                     /locus_tag="VWX16_000070"
     CDS             complement(76091..76906)
                     /gene="pstA"
                     /locus_tag="VWX16_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9157516.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(76899..77762)
                     /gene="pstC"
                     /locus_tag="VWX16_000071"
     CDS             complement(76899..77762)
                     /gene="pstC"
                     /locus_tag="VWX16_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9157517.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYSFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(77880..78755)
                     /locus_tag="VWX16_000072"
     CDS             complement(77880..78755)
                     /locus_tag="VWX16_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9157518.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(78922..80253)
                     /locus_tag="VWX16_000073"
     CDS             complement(78922..80253)
                     /locus_tag="VWX16_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9157519.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAKYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(80254..80961)
                     /locus_tag="VWX16_000074"
     CDS             complement(80254..80961)
                     /locus_tag="VWX16_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9157520.1"
                     /translation="MTKQVLLVDDEEHILRLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGIKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(81045..81530)
                     /locus_tag="VWX16_000075"
     CDS             complement(81045..81530)
                     /locus_tag="VWX16_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9157521.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERADLLATYPEHKEMIEHLTR
                     Y"
     gene            81516..82073
                     /locus_tag="VWX16_000076"
                     /pseudo
     CDS             81516..82073
                     /locus_tag="VWX16_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(82463..84154)
                     /locus_tag="VWX16_000077"
     CDS             complement(82463..84154)
                     /locus_tag="VWX16_000077"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9157522.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            84302..84748
                     /gene="argR"
                     /locus_tag="VWX16_000078"
     CDS             84302..84748
                     /gene="argR"
                     /locus_tag="VWX16_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9157523.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            84799..87333
                     /gene="mutS"
                     /locus_tag="VWX16_000079"
     CDS             84799..87333
                     /gene="mutS"
                     /locus_tag="VWX16_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9157524.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREESA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQYLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            87480..89174
                     /gene="patA"
                     /locus_tag="VWX16_000080"
     CDS             87480..89174
                     /gene="patA"
                     /locus_tag="VWX16_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9157525.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SNELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(89492..89755)
                     /locus_tag="VWX16_000081"
                     /pseudo
     CDS             complement(89492..89755)
                     /locus_tag="VWX16_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            89949..91715
                     /gene="patB"
                     /locus_tag="VWX16_000082"
     CDS             89949..91715
                     /gene="patB"
                     /locus_tag="VWX16_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9157526.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            91854..92543
                     /locus_tag="VWX16_000083"
     CDS             91854..92543
                     /locus_tag="VWX16_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9157527.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTYHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            92547..92726
                     /locus_tag="VWX16_000084"
     CDS             92547..92726
                     /locus_tag="VWX16_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157528.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTRKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            92713..94062
                     /locus_tag="VWX16_000085"
     CDS             92713..94062
                     /locus_tag="VWX16_000085"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9157529.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDKKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            94186..95646
                     /locus_tag="VWX16_000086"
     CDS             94186..95646
                     /locus_tag="VWX16_000086"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9157530.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            95660..96094
                     /locus_tag="VWX16_000087"
     CDS             95660..96094
                     /locus_tag="VWX16_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9157531.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGKERASKFNER"
BASE COUNT        28462 a        18014 c        20965 g        28689 t
ORIGIN      
        1 gaagttgtat gtaaatattg aggaaataaa aatggaaagt aaaaaaatag caaaacagat
       61 tttaatagct acggctgttt taacaagttt tttaggaagt aatttggttt atgcagatgt
      121 agttcaaagt aattctaata acagagctag tactgaaact gctagagtaa cagggaataa
      181 cttagaaaaa cttattacta aagataaaga aattgataaa gagatgacat atctttcaga
      241 tatggattgg tcttctgcaa ctcatggtga tatagataaa acaaaaacag tacaaaaaga
      301 tgctccattt acaacaggaa ataaaggaga gcatactaag atttcattgc tcacttctga
      361 tgataaagtt aaatattttg ataaaggaat aggaacagtc gcagattctc catcagtaat
      421 cagctatgat atcagcggtc aagggtttga aaaatttgaa acttatatag gaattgatca
      481 aagtgctaat agttctagat cagaccatgc agtagtggat agaatagaaa tagaaattga
      541 tggaaaggtt gtctattcta gtagtgtaac taatccagaa ggattcagat ataataccca
      601 ggcacaattt attagtgtga caattccaca aaacgctaaa aaaatatcct taaaatcatt
      661 cgcaggagaa catacatggg gtgatgaggt tgtatttgct gatgctaaac ttattaaaac
      721 tgttagtact caaactataa ctccagatct tcttaataaa ggaattaatg gaggagtata
      781 tctttctgat ttagaatggg ttgatgctac tcatggtgat gacgataaat caaaaacagt
      841 tcagaaagat aaaccgttta cacctggtaa taacgggtct aataataaaa taaaattact
      901 aattgacggt aaggaagtcg aatttaataa gggattggga actgtagcaa gcaacccgtc
      961 tagtataaaa tatgatgttt cgggtgctaa tgttacaaga tttatttcct atgtcggaat
     1021 tgataggagc gccaatcatt taaattcaga ttacgcagat attcaaaaat ttgaagttgt
     1081 tgcggatggt aaagtcattt actcatcaga ttcaaaatat ccaaaaggaa ttaagtatga
     1141 tacatcggca tttctggttg atgttgaaat tcccaaagat acacagacaa tagaacttaa
     1201 atcatattca gggaaacata catgggctga cgaattggtt ttaggtggag ccttgtttat
     1261 ggcaaatggt aaatttaaga atcctaatga ttggtcagaa gttgataaac gtcgtgaaat
     1321 taataatgaa catccgttac ttatgatgcc tttatatgct aacggagaag agtttaacca
     1381 agggaaatat actttttggg gaggagatac attaacaggt aagtgggaga atataccaga
     1441 tgacttaaaa ccatatactg ttatacagtt acatccagat gatcttccta aaagagatgg
     1501 agctgctaga gatttttatg aacatatgtt agaagaagca gcgaaatatg taaatcctaa
     1561 gactggaaaa aatgaaccaa ttccagttat tctgacggtt tacactgctg gtaatatgcc
     1621 ttattatact tcggcacatt ggttaagtac cagctggatt gataaaatgt atcaaaaata
     1681 tccaaatttg catggtattt tcagtacaga gaattattgg atttgggcaa atgatattga
     1741 aaataaggcg gcagattact taaaagtatc agctaaaaat ggaggatatt ttatatgggc
     1801 tgaacaaaat aatggttctg caatagaaaa agcatttgga aaaaacggta agatagcatt
     1861 tcagaaatct gttgataagt actggaagaa ccttattttc atgttcaaaa atacacctgc
     1921 tgctgaggga aatgattcaa ctacagaaag ttatatgaag ggactttggc tttcaaatca
     1981 tacttatcaa tggggtggtt tgatggatac ttggaagtgg tatgagacag gtaaatggaa
     2041 actgtttgca agtggtaata tcggaaaaag tcaaggagat agacaatggt tgactgaacc
     2101 agagtctatg ctaggagaag aagctttagg ggtttacctt aatggaggtg tagtttataa
     2161 ttttgaacac ccagcttata cctatggtgt aaataataaa gaaagcttac tctttagcga
     2221 agtaataaaa gaatttttta gatatgtaat agcgcatcca gcaccatcca aagaaaaggt
     2281 acttgaggat acaaaagtat tcatacatgg agactattca aataaaggaa atggtaagtt
     2341 ctttgtaaac gtaaatactg atagagaaca aactccatta tacatgactg gtcgttataa
     2401 tgttattcca gctattccag gtgttttaaa aacagataaa ttgaaagagt ctgtttctag
     2461 tagccgtatt cagattaagg aaatcacttc tccagaattt agctctactc aggctagaaa
     2521 agaatactta aataagctgt atcctatgaa ttatgaagga gatatctttg ctcagaaatt
     2581 agataataga tggtttgtct ataattacaa ggtaaatgag aatgttaaac agactgggaa
     2641 attaaaattt aattctttag aaatgaatgt tgaatttgaa cctcatacct atggtatttt
     2701 tgagagaata agtaatggat tgaaagtgaa tttaaataat tttagaacaa ataaagattc
     2761 actatggtct aatgctcaag atgcaaatca ggcaaagaaa ctaccacaat tgaccaagaa
     2821 aggtgcgatt aagtggattg aagaacatta tatcaaagat actcagtttg gagagaaaag
     2881 ggttacaaag atagtattaa gaggtataga taaacttcct actattcatt cattatcagg
     2941 aacaaataat tcctatgatc agccatctct caattttgat cagaaaaatc atatggttac
     3001 tattaccatt aacagtaacg gaaatcttga attcgaactt catttttaga aaggagtgag
     3061 tttatgtctc aagatgaaaa attaattcgt gaacagattt gtgatgtttg tcataagatg
     3121 tggcaacttg gttgggttgc tgctaacgat gggaatgtat ctgttcgatt agatgaggat
     3181 accattcttg caacacctac tggtatcagc aaaagtttta ttacaccaga aaagctggtg
     3241 aagttaaatc ttaaaggaga gattttagaa gcagaaggtg attactgtcc ttctagtgaa
     3301 attaaaatgc acattcggtg ctacgaagaa cgtgaggatg tccgttcagt tgttcacgcg
     3361 catccaccga ttgcaacagg atttgctctt gcacacattc ctttagatac ttattcacta
     3421 attgagagcg cgattgtggt aggagcaatt cctataacgc catttggagt tccgtctaca
     3481 atggaagtgc cagaagcaat tacaccttat ctgcccgatc atgatgtcat gctattagaa
     3541 aatcatggag ctctgactgt cggaagcgat gtaattacag catactaccg tatggaaact
     3601 ttagaattag tcgcaaagac aaccttccac ggaagaatgt tactttctac aaagggcatt
     3661 gaggagcaag aaattgctcg tccgacttta gaacgtctat tctcaatgcg agaaaattat
     3721 aaggttacag gtcgtcaccc aggctaccgt aaatataatg gcgatggtag tatgaaagaa
     3781 acagaaaaat aagaggaaaa tagtatgatt caacatccac gtattggtat tcgtccgact
     3841 attgatggtc gtcgtcaagg tgtacgcgaa tcacttgaag tgcaaacaat gaacagggct
     3901 aaaagtgtgg cagatttgat ttcaagcaca ttgaaatatc cagatggaga acctgtggaa
     3961 tgcgtgattt ctccatctac tattggccgt gtaccggagg ctgcagcttc ccatgagttg
     4021 ttcaaaaaat caaatgtttg cgcaacaatt acagttacac catgctggtg ttatggtagt
     4081 gaaactatgg atatgtctcc agatattcct catgctattt ggggatttaa tgggacagaa
     4141 cgcccaggag ctgtctatct tgcagctgta ctagcttcac atgctcaaaa agggattcca
     4201 gcctttggga tttatggtag agatgttcag gaagctaatg atacagctat tccagaagat
     4261 gtcaaagaaa aacttttacg ttatgcgcgg gcagctcttg caactggctt gatgagagac
     4321 actgcttacc tatcaatggg tagtgtttcg atggggattg gtggttctat tgtaaatccg
     4381 gatttcttcc aagaatactt aggaatgcga aatgaatcgg tagatatgac ggagttcaca
     4441 cgccgtatgg accgtggtat ttacgaccct gaagagttcg aacgtgcgct caaatgggtg
     4501 aaagaaaacg taaaagaagg attcgaccat aaccgtgaag accttgtttt aagccgtgaa
     4561 gaaaaagata gacaatggga atttgttatt aagatgttca tgattggacg tgacttaatg
     4621 gttggtaacc caagacttgc tgaccttggt tttgaagaag aagcagttgg tcaccatgct
     4681 ttagtagctg gtttccaagg tcaacgccag tggacagacc atttcccaaa tggggacttt
     4741 atggaaactt tcctcaatac tcagtttgac tggaatggta ttcgaaaacc atttgtattt
     4801 gcgacagaga atgattcact aaatggtgtg tctatgctct ttaattatct attaacaaat
     4861 actccacaaa tatttgctga tgtgcgtact tattggagtc cagaggctgt taaacgtgta
     4921 acgggacata ctttagaggg tcgtgctgca gctggcttct tacatctaat caactctggt
     4981 tcttgtacat tggatggtac aggtcaagct attcgagatg gcaaacctgt tatgaaacca
     5041 ttctgggagt tggaagaaag tgaagtgcag gctatgcttg aaaatacaga cttcccacca
     5101 gcaaaccgcg aatacttccg tggaggagga ttctcaactc gtttcttgac gaagggggat
     5161 atgccagtaa caatggtacg tctcaatctt ctaaaagggg ttggtccagt gctacaaatt
     5221 gcagaaggtt atacacttga acttcctgaa gatgttcacc atactttaga taatcgtaca
     5281 gatccaggat ggccaactac ttggtttgct ccacgtttga caggaaaagg tgctttcaag
     5341 tctgtctatg acgtcatgaa taattgggga gctaatcacg gagccataac atatggacac
     5401 attggagcag acttgattac cttggcttct atgttgagaa ttcctgtcaa tatgcataat
     5461 gtacctgagg aagatatctt tagacctaaa aattggtcct tatttggaac agaagatcta
     5521 gaatcagcag actatcgtgc atgtcagttg ttgggaccac tacataaata aaacttgttt
     5581 atataggagg tgaacttacg tccctcctat ccttttaaaa agatttgtta aacaattcac
     5641 aaataattaa aaacgaatac aaaaagtaat ataatgatgt taaatagata gcgcggaggc
     5701 gcaggaggaa aattatatgg ctacatttta tgttccggca gtcaacctta ttggaaaagg
     5761 tgttgtaaat gaagtgggtc cttatatcaa ggaacttggc tataaaaagg cacttttggt
     5821 gacagataag tacatcgaag gcagtgatat tttacctaag actttaaaac cactggatac
     5881 agaaggaatc gaatatgtca tctttagcga tgtagagcca aaccctactt gtaaaaatgt
     5941 cacagatggg gtagctgctt tgcaagaaca tggctgtgac tttatcatca gtcttggcgg
     6001 gggttctcca caggatgcag ctagttgtat ttctatcatg gctacaaatg gtggaaaacc
     6061 acaggattat gaagggcttc ataagtctgc taaaaaaggc ttgccagttg tggctatcaa
     6121 tacaacggca ggaacttctg cagaaatcac tattaactat gtgattactg atgaagaacg
     6181 caaggttaag atggtaatgg ttgacaagaa tagccttgct cttatctctg ttaatgaccc
     6241 ggaactcatg ctttccaaac ctaaaggcct gactgctgct actggtatgg atgctctgac
     6301 tcacgctgtt gaagctttgg taacacctgg tgcttatgat gtaaccaaga aactgtctat
     6361 tggtgctatt gagcttatca aggaatatct tcctcgtgct gtagaaaatg gacatgatat
     6421 tgaagcgcgt gaaggtatgg tcaatgccat cttccttggt ggtatgagct ttaataatgc
     6481 tggtcttggt tatgttcact caatggctca ccaactcggt gcagtatata atttgccaca
     6541 tggtgtttgc tgtgccatgt tgctaccagt tatagaacgt gaaaatgcta aacgtgtacc
     6601 agaagctttc cgcaatgttg ccaaagcctt gggacttcat gtagaaggta aatcagatca
     6661 agaatgtgcc gattatgcga ttgttgagat tgagaagctt tctgagacag taggtattcc
     6721 taagaaactg actgaacttg gtattgaaga aaaagatttc gactttgaat acctttctaa
     6781 gaatgccttg attgatgcct gcgcaccagg aaatccattt atgccaacct tagaagaaac
     6841 gattgccttt tataaagagt tattttagca agagattgga cgacaatagg acaattcacc
     6901 tctaattatt gacatttcag gaaaaatcgc agtaaaaacg gcacagcctt ctttaaatgt
     6961 gctataatac tagaaaaata cttgtggagg ttccattatg gcaatatttt tcatgatttt
     7021 tctgattgtt tgtgtgctcc tattggtgat agtcacactg agtacagttt atgtggttcg
     7081 tcagcagtcg gtggcgatta ttgaacgctt tgggaaatac caaaaggttg ctaatagcgg
     7141 tattcatatt cgcttgcctt ttgggattga ctcgattgca gcacggattc agttgcgctt
     7201 gttgcaaagt gatattgtgg ttgagactaa gaccaaggac aatgtgttcg ttatgatgaa
     7261 tgtagcgact cagtaccgtg tcaacgagca gagcgtgaca gatgcttact ataaactcat
     7321 acgtccagaa tctcagatta aatcttatat cgaagatgct cttcgctctt ctgttccaaa
     7381 attaaccttg gatgaattgt ttgagaaaaa agatgagatt gcccttgagg ttcaacacca
     7441 agtagcagaa gaaatgacca cttacggcta cattatcgtg aaaaccttga ttaccaaggt
     7501 cgaaccagat gcagaagtta agcaatctat gaatgaaatc aatgcggcgc aacgtaagcg
     7561 ggtcgcagca caagaattgg cggaagctga caagattaaa attgtcactg cagctgaagc
     7621 cgaagcagaa aaagaccgcc ttcatggtgt ggggattgcc caacaacgta aggcgattgt
     7681 ggatggattg gcagagtcta tcaccgaact caaggaagcc aatgttggca tgacagaaga
     7741 acaaatcatg tctatcctct tgaccaacca gtatttggat accttgaata cctttgcctc
     7801 taaaggaaat caaaccatct ttttaccaaa tacgccaaat ggtgtggatg atatccgaac
     7861 acaaatcttg tcagcccttc gcgctgagaa gaaataatag actaatactc ttcgaaaatc
     7921 tcttcaaacc acgtcagcgt cgccttgccg tatagatgtt actgacttcg tcagttctat
     7981 ctgcaacctc aaaacagtgt tttgagctga cttcgtcagt cttatctact acctcaaaac
     8041 ggtgttttga gcaacctgtg gctagtttcc tagtttgctc tttgattttc attgagtaca
     8101 aagagcttgg caacaggctc tttttgtatt gcgtttatta agaaaaacaa aaaacatcga
     8161 tatacattta actaaaaatt attgtatgtt atctccctta attaggaatg ataagggaat
     8221 aactagaaag atttgtgaat acaaactatt tctgatatac aaaatataca gtaataatga
     8281 atgatgggag atgggatgaa agaatttcaa tttgagagaa agcagcgttt ttctttgagg
     8341 aaatatgcaa taggagcttg ttcggtcttg ctaggaacga gtttattttt tgctggtatg
     8401 ggtgctcagc ctgtacagga tacagaaacg agttcagcac taatttcaag tcattatttg
     8461 gatgagcagg atttatctga aaagctgaaa tctgagttgc aatggtttga attagaaaac
     8521 aagcttttga acttatggga gcattaggtt actatgaagg atttgttcct tacgtttcaa
     8581 atcaatacaa aaaccaagct gaagaagaag acaaaccgct atctgataaa tatattttcg
     8641 aaaaaatctt aggaaaaaca tatgcagcct tcaaaaaaga tcaaattaat gagcgtgtcg
     8701 agaaattagg taagttaaaa cctattacaa taaattacaa cggaaaatca gaagtaattg
     8761 atagtaaaga aaaattacaa gagcttatga ataaagccgt taaagacgaa gtggctcaaa
     8821 tatagtaaaa tgaaataaga acaggacaaa tcgttcagga cagtcaaatc gatttctaaa
     8881 aatgttttag aagtagaggt gtactattct agtttcaata tactataaaa tcaggcaata
     8941 ccacggctaa aaagtttgag tttatcgaaa caccagtgca aaaactgaaa aaagcgattt
     9001 ataaagctca tctcaaagat tcagatgact ttagaccaga aacatccaca ccaaatcttt
     9061 ttgaaagctg tttgaagctt tgtccttgct ttcttagttc atagatctga actttatctt
     9121 cataactcaa tttcataata aaacacccca aaagttagat ttattctgtc taacttttgg
     9181 ggtgcagttc attttgcggg attttttttg gtagaaacag ttgcctgatt aagttgcaag
     9241 tcgatagacg gcttcggcat aaatatccat agcacggtac aaatcttcta gaacggcaca
     9301 ttcatttgcc tgatgttctg tctgcttcgc tcctgggaat aaggcgccga aggctacaca
     9361 atttggcatg gtgcgagcaa aagtggcacc accggatgaa atagcaggac tgttatcgcc
     9421 agtcttttct tggtagattt gcatcagtgt gctaacgagt ttactgtctt ttgcgacgta
     9481 tagagacgct agatagtcaa actcttcgta gcggagttgg tagttttgtg cacatcttgt
     9541 aagcaactct actagttctt ccttgtcagc taagacagga gtccgaatgt caatacggat
     9601 ttcagaacgt tcatgattga tcatgagacc tgcgacatta aaggataggt gaccagaagg
     9661 ttcatatgct atatcaccaa agatttgtct tcctgtgccg tcttgacctg cttgtgttgc
     9721 aagaaagctg agagcagggt gttcttggag aggagcaaga atggtagcta gtcggatgac
     9781 agcattgata ccttgactag catccttagc atgctttggc actccgagaa ccgttacggt
     9841 ttgttcagtg gattggtaat cataaccagc ttctttgaga ccgttacaaa cctgttcata
     9901 gaggggacct tggtagttgg ccttgtctgg tacaacgtta aaggcgcctc ctacttcaag
     9961 ctctagttga tccgatccag ggccatgaag tttgacctgt agaagccctt tttcagcata
    10021 ggtcagagga aaagatgagt caggtgcaaa gcccatactg gcctgttctt cgatggtatt
    10081 gtagcgtgcc atgcagcgcc agagggtttc ctcatcggta ccaaagataa agcgtacgcg
    10141 ctttttgaac tgaatacctt ggtccagcaa gctttttact gcatagagag ctgcgagcga
    10201 agggccttta tcatcttgga caccacgtcc gaatacccag ccgtctttga tagttgcttc
    10261 aaatggcggt gtctgccaat ctgcttcatc acctgatgga acaacatcca aatgacagag
    10321 aatggccaga agctctgctc cctgaccgat ttctgcatat ccgtaataac ctttagggtc
    10381 aagataggta gtgaaaccta tgtctcgaca aatctctaaa gttttttcta ggacatcttg
    10441 gattgcttgt ccaaaaggtg ttccattttc tccttcattg agtactgaag gataggaaat
    10501 caaggttttt aatgatataa gaaattcatc tttaacttga tttgttatat ctattttcat
    10561 ggaatcacct cactcactat tttataggaa tggaaggaag gttccaagga gaagaagggc
    10621 aatggtcact acaataatag cgactacgag tttgcccatg aatttccacc aagtaccaat
    10681 gttgatacgt ccaagtgcaa gagctcccat cacaatacca gatgttggtg caatcaagtt
    10741 caagacacct gaagcagatt ggtaagcagt gataatcaag ctaggacgga catttacaaa
    10801 ttctccaagt ggagccatga tacccatagt tgcgctggca agaccagatg aagatgggat
    10861 caagaatgac ataggtagat agaagatata agttacaacg ataaagactt gtgaagatag
    10921 accgctcaag ccttctttac cccagttgag gattgtatcg gtaatcatac cgtcgttcat
    10981 gataacttga ataccacgag caatcgctac gatcaaggca acactgagca agtcagcagc
    11041 accattcatg aaggaagaga taatcttatc ttctttaaga ccataaataa caccaatcag
    11101 gatacccata aaggcaaaga gcattgcgcc ttctgggaag taccaagtac ctagtgcaga
    11161 agtagatgaa ccgacaatat taccaataac tggaagacca gtcaaccaag cattaaagtc
    11221 atcaaaaatg gtaacgccaa ggtctgtcca tggaatgaag ctcaatacca tcaagatgaa
    11281 tgtcaacaca aataagaaga gaactgattt ttgtttgctg ctaagtgtag attctacaga
    11341 tgaagattct tctacgttaa agtgtttcaa atcttctttg cgagtactat aaaccagtga
    11401 cttagtcgga tctttttgaa tcttatccgc ataacggtaa acaaaccaag tactaagagc
    11461 agtcaaggta acccagaaga tcagacgaag tacgataccg tcccctgtac caactcccgc
    11521 agtcgctgaa gcaatacctg tcgcaaatgg attcagagta gatgccaaac agccgatttg
    11581 agaaccgagc aaaataattg caacaccagt caggctatca aaaccaacgg ccatcataac
    11641 tggcacaagg agtggataga aggccattgt ttcttcaccc ataccataag ttgtaccacc
    11701 gagggcaaac aaaggcatta gtaccaaaat taacattttt tcgcggccct tatacttctt
    11761 cacgatagag gcaatcccta cgtcaagagc accagttttg ttgacaatgc caaggaaacc
    11821 accaaccata aggatgaaga aggctacatc aatcgctgcg ctcgtttctt taatgagcga
    11881 accttcctct ggatgagtac ctagcatagc ccgaatcggt gccatgagga catcccaaat
    11941 cccttgtgga ttttgaggct gagtctcgta aataccttct ataaaggccc ccgcagggat
    12001 aaaccaagtt agtactgcca taatagcaat gattatcaat aatacggtgt aagatgaagg
    12061 catcttaaac ccttttttag ctttttcact catttgtttt tcctcctgat gtctaacaaa
    12121 gtgtaaaaaa ccaactactt tcttaaataa acatttaaga aaacgctttc tatttatatt
    12181 tgttttacca tattaaagtc taaaaaacta atatcttttg aaatattgta catgttttca
    12241 agaataattt ctaatttgat ataaagatac tagaaataga atacatctta ttagtaaaac
    12301 aacttatcct ttttcaataa ttgttccact ttcagattca atcaaggcgc ctagattttc
    12361 aagggaagta ataactgctt ttccttctgg acgaccattg acaaaagcga tagctgcttc
    12421 tacttttgga agcatgctac ctggtgcaaa ctgatcttgt ttgatatatt cttccagctg
    12481 ggcaacattc acatgttcca atttttcctg gtttggcttg ttgtagttaa caaatacata
    12541 atctacacct gtcaaaacga tgaagaggtc tgcatcaacc aattctgcca aacgttggga
    12601 agcgaagtct ttatcaataa ccgcttcgac accagtcaaa tgtccattgt tttctttgac
    12661 gacgggaata ccgccaccac ctgcagctac gacgacttga ccattattta aaagagtacg
    12721 gatggtttca atttctttga tgtcaacagg ttttggtgag gcaacgacct tacgccagcc
    12781 acggccagca tcttccttga aagtcgctcc gctcttttcg gcttctgctt ttgcttcttc
    12841 ttctgaatag aaaggaccga ttggtttact caagttaaca aaagctggat catttttatc
    12901 tacgacaact tgcgttacaa cagaggcaac atttttttcg atgccttcat ccaagagagc
    12961 attttgcaaa gcatttttca accagaaacc gatgctacct tctgtcatag cgacaagtga
    13021 gtcgagtggg aaggcagggt tcttttcaga gtctgatgcc aaatgttgga gcaagagatt
    13081 cccaacttga ggtccattac cgtgagtgat aatcaaatca tctccatttt taatcaattt
    13141 tacaagatgc ttagctgttt caactaaagc ttcttgttga gcctttgctg atgggtcaga
    13201 agaaagaatc gcatttcctc ccaaagctac tacaatttta cgatttgcca taaattctcc
    13261 tttatcacac tcaatagaat gcgtttagat ttcaatttaa tgatttttca catattttat
    13321 aagaaataat agattaccat tacataaaag aggaccggac taaagctatt agtcgcagcc
    13381 ctcatagctg ttggtagacg gtttattatc taaaattata ctttaggaat ataaaggtta
    13441 ccaagtgtcg cagccataac agctttgata gtgtgcatac ggttttctgc ttgatcgaag
    13501 tggcgagcgt acttgctgcg gaagacttcg tctgttactt ccatttcttc tacaccaaat
    13561 ttttcagcaa cgtctttacc ataaacagtg tgagtatcgt ggaatgctgg caagcagtgt
    13621 aggaagatca agttttcatt gcctgctttt ttaactaagt ccatattgac ttggtaaggt
    13681 ttaagaagag ctacacgttc tgcgaatttg tcttcttcac ccattgatac ccaaacgtct
    13741 gtgtaaagaa cgtctgcatc tttaactgct tcatcagcat cttcagtgat gagaacatgt
    13801 gcgccacttt ctttagcaaa tccttctgcc aattcaacga tttctttttc tgggaagagt
    13861 tcttttggtg agaagatgtg aacattgaca ccaaggatag cacctgttac gagcaagctg
    13921 ttggcaacgt tgttacgtcc atcaccacag tataccaatg tcaagccttc caagcgaccg
    13981 aagttttctt gaacagtcaa gtagtcagcg agcatttgag ttgggtgcca ttcgtcagtt
    14041 agaccgttcc atactggaac gcctgagaat tctgccaatt cttcaaccat acgttggctg
    14101 aatccgcgga attcaatccc gtcaaacata cgtcccaata ctttagcagt atcttcagta
    14161 gattcttttt tacccaactg aatatcattt gctccgaggt attctgggtg agcaccaagg
    14221 tcgatagccg cagttgtaaa ggctgcacga gtacgagtag atgttttttc aaataggaga
    14281 gcgatattct tgccagcaag gtagtggtgt tgaatattgc gttttttcaa atctttcaag
    14341 tgagctgaaa gaccaataag gtattctaac tctgcacggg taaagtcttt ttctgctaag
    14401 aagctgcgtc cttggaatac tgaatttgtc attttattat ttcctctttc tattttttac
    14461 attttctatt gacgaatacc gaacagcgat tacacttctt cacgttcaaa tggcatagac
    14521 atacaacgag gtccaccacg gccccgaacc aattcacttc cgcgaatctt aatcaagcga
    14581 agcccgtatt cttccaaaat cttattggtc acggtattgc ggtcataaac aactaccaca
    14641 ccaggtgcga tggtcaaagt gttagaaccg tcgttccatt gttcacgcgc agctgctacg
    14701 atattgccac caccgcaacg aatcaaatga actttttcta caccaaggtt ttgagcaaga
    14761 agttcagcta agtcaccttt ctcttcaacg attttaagtt tttcgttttc gtaagtaact
    14821 gagtaaacgt gaaggtcgcc ttcgatttct gggtgaatag tgaacttgtc atagtctacc
    14881 atagtgaaga cagtatccaa gtgcatgaat ttacggttgt tagcaaattc aaaggccaaa
    14941 actttcttga agccaacatt tttcttgaag atgttgacca aaagtttttc gatagaagct
    15001 gcgtctgtac gttgagagat acctactgca aggacgtctt tagaaagaac tagctcgtct
    15061 ccaccttcga tacgcgtatc ttcttcacgg ttgtagacca aatccacttt tccgccatag
    15121 attgggtggt atttgaagat atacttaccg tagagtgttt cacggttacg agtgtctgca
    15181 aacatgtggt taagcgatac ggcgtttcca attgttgcaa atgggtcgcg agtgaaatag
    15241 aggtttggca tcgggtcaat tgcaaatgga taatctgatt caactaagtc agttagatct
    15301 ttagcttcgt caggaatttc tggcaattca actttttgaa tcccagccat tgttttttca
    15361 accaattctt ggttgtcctt gatgccgtga agcaattcac gaatagcaac cttggtttga
    15421 cgatcacgga tgttggcttc gtctaagtat tcctcgataa attgatcgcg gatttctgga
    15481 gaggtcaatg attcagcagc gagttgttct aggtagagaa cctcaattcc ttcatcgcga
    15541 agagcttggg caaatgcatc atgttctttt tgagcatctt ccaagaaagg aatatcatca
    15601 aaaagaagcc tttcaagata gtccggcaac aagttttcta actccttgcc tggacggtgc
    15661 aacataactt ttttcagttt cccaatttct gagaagacct gaattggatg tgaagacatg
    15721 tattttcctc cttaaaaatt ttgacttggc accattttgg gtaccgctta catgtatgtt
    15781 ataacgcaat tcctaggtaa tgtcaatggc tataatttct cggaaaataa tataaattat
    15841 gatgtttagc acaattttat gttattatac ataacatagg atagaataca tagatacaaa
    15901 tgcataaact tctataaaaa ggatataaaa tttttttaaa aaatttttca aaaaatatac
    15961 aaatgaccaa aaagtgtcca attatcatct ggttatcact tggatctgta taaagagaaa
    16021 tgtttgtgtg aaaggataaa aagatgcaac attcttttga agatagattt tggagaagca
    16081 tttcttgttc aattgtacaa attatagaat cttttctgag tatttaggaa gagataatta
    16141 tcttattgac cctcttttta ttttttgtta tagtcgacga ggtgatagct tagaataatc
    16201 acttattcac aatctgtgga aaagtctgga gagtgaatgg tcttcacaca atgacctgaa
    16261 agaagcctga gaataattat ggagagtagc attctgagag gtgttagcag aaccatatga
    16321 cagagctgtt tgaagaggga atattgagga gaaaaatcct gagcctacca gttggagttg
    16381 gaaagagctg gcggttagat cattttttat tatccacaac ctgtggataa ccttgtgaac
    16441 aagagaagtt gctaaagaag gagatatata acgatgaaga aaatcaaacc gcatggaccg
    16501 ttaccaagtc agactcagct agcttatctg ggagatgaac tagcagcttt tatccacttc
    16561 ggtcctaata ccttttatga ccaagaatgg gggactggac aggaggatcc tgagcgcttt
    16621 aacccgagtc agttggatgc gcgtgagtgg gttcgtgtgc tcaaggaaac gggcttcaaa
    16681 aagttgattt tggtggtcaa gcaccacgat ggctttgtcc tttatccgac agctcacaca
    16741 gattattcgg ttaaggtcag tccttggagg agaggaaagg gcgacttgct ccttgaagta
    16801 tcccaagctg ccacagagtt tgatatggat atgggggtct acctgtcacc gtgggatgcc
    16861 catagtcccc tctatcatgt ggaccgagaa gcggactaca atgcctatta tctggctcag
    16921 ttgaaggaaa tcttatcaaa tcctaactat gggaatgctg gtaagttcgc tgaggtttgg
    16981 atggatggtg ccagaggaga gggcgtgcaa aaggttaatt atgaatttga aaaatggttt
    17041 gaaaccattc gtgacctgca gggcgcttgc ttgatttttt caacagaagg caccagtatc
    17101 cgctggattg gcaatgaacg agggtatgca ggtgatccac tgtggcaaaa ggtgaatcct
    17161 gataaactag gaacagaagc agagctgaac tatcttcagc acggggatcc ctcgggcacg
    17221 attttttcaa tcggagaggc agatgtttcc atccgtccag gctggttcta ccatgaggat
    17281 caggatccta agtctctcga ggagttggtc gaaatctact ttcactcagt agggcgagga
    17341 actccactct tgcttaatat tccgccgaat caagctgggc tctttgatgc aaaggatatt
    17401 gaacgacttt atgaatttgc gacctatcgc aatgagctct ataaagaaga tttggctctg
    17461 ggagctgagg tatctggtcc agctctttcc gcagactttg cttgtcgcca tttgacagac
    17521 ggccttgaga ccagctcttg ggcaaacgat gcagacttgc ccatccagtt agaactcgac
    17581 ttaggttctc ctaaaacttt tgatgtaatt gagttaagag aagatttgaa gctagggcaa
    17641 cgaatcgctg cttttcatgt gcaagtagag gtggatggtg tctggcagga atttggcagg
    17701 ggcttcacag ttggtcataa gcgcctcttg cgaggtccgc tagtagaggc acaaaaggtg
    17761 cgcgtgatga ttaccgaggc acaatctatt cctgtattga tcaagatttc cctttataaa
    17821 actcctggat tatcaaaaaa agaagttgtt caggaactag catttgcaga aaaaagccta
    17881 gctgtggcaa agggagagac tcttcatttt aggattgaac gtagtgaaag tagctcctct
    17941 ttagaggcta agatttcgat tcaaccgggg acaggtgtcc atggtgtcgc ctatcaggac
    18001 gagatttatg tccttgcgtt tcaagctggt gagactgaaa aaaggctgac gctaccaacc
    18061 ttgtatttcg caggagataa aaccttggat ttctatctga acctaacggt ggatggtcag
    18121 cttgtggatc aacttcaagt ccaagtttca taaaagaaga acctttgcgc gatgcaaagg
    18181 ttcttttggt tattagtgat ttggtagcca gctgagggtg aaagttagtt gttcagcttt
    18241 taggaggtct tggtgttgaa tagttgatac gagtgttttg tccagtcggc attctttgac
    18301 aaagttaaaa tggctgtggt tttgctcagt atgaatatct agccatttat tttctttagc
    18361 taggtagact cgtagatggt caaagagagg aattccgaga tcatagcttg gttttcctgg
    18421 acaggttgga taaaatccga gagctgacca gatgtaccaa gcagagagac taccattgtc
    18481 ttcatctcca ggataggctt cccaacttgg gtgaaaagct ttctgacgga gcgtcttgat
    18541 aagaagggca gtgtagtcag ggtaatcgct gtagcggaag agataaggaa tgtggaaact
    18601 aggctggttg gaaatggcta tttgtccaaa aggagcagta gccatctcgc tcatttcgtg
    18661 aatttcgtaa ccatagcctg ttgtttcaaa gaggggagca tcttgacagg ctttcaaaag
    18721 atagttgcta aaggtttctt ttccacccat cagttggatt aagccaggga tgtcgtggag
    18781 aacgcctaaa gtagcttgaa tggcagagca ttcagcgtag tctcgccccc aactataagg
    18841 agagaagtca gggtgaaagt ttccttgatt gtctcgtgct cgcatgtaac ctgtctcagc
    18901 gtcaaatagc tggcggtaat tttgtgaagc agccttgtag gtttcagcga tttctatgtt
    18961 ctctagtttt ttggcacagc tggcgataca aaagtcacta taggcatagt ctagagtatg
    19021 gctaacactt tcgtggtggt cggtagagag gtaacctagt tcttggtatt gggctagtcc
    19081 gtggcggcca ttgattccga gagggtcggc tttgctggct gtttcgagca tggcttggaa
    19141 gagttctcct tctaggtcag gggccatgtc cttgcaggcg ctatctgcga taataccgtc
    19201 taaaagtgta cctggcatca taccccgttc atctggagcc agccattttg gaaggaaacc
    19261 agtatcgcgg tagctattga ggaaaccttc taaaaagcgt tgatagtgct ccggtatgat
    19321 aagggcaaag agggggaagg tggtgcggaa ggtatcccag aaaccattgt tgctaaagag
    19381 gacaccaggc ttgacagtac cagtagccag atccatgtgg atggcttgcc ctgattcatt
    19441 aatctcataa aaagtctgtg ggaagaggaa gagtctgtag aggcagtggt caaagaaggt
    19501 tcggtcagcc tctcctatct ctataatgtc aaaacgatgg aggagatttt cccaatccgc
    19561 ttgggcactt gatttacagc tatcaaaatc ttcttgaggt agattaagca gtgcttgaga
    19621 aggagagatg aaagaagtgg ctagttgcat ctcggtttga ctacttgcta agtcaattcg
    19681 ccagtctcca gcttcttggc tgatagcaag aatatccgtg ttcatttgca gggcagtgaa
    19741 gagcatcagt ggatttttat tggtctctgt tttgccttct tgtctgagag ccagagtccg
    19801 cttatctacc tgctcaactg ttagttcatc tgctgcgtga agatagaggg agagggcttt
    19861 gccttgcttt tgcttcaaac gaatagaagc accatagcaa gtcggtgtga gctgggtttc
    19921 aatttgatag cgcagggaaa agagcttcaa atagtgaggc tggaagcaag ctttatctat
    19981 atcataagaa gactggcggt gaaagaggct gtctccaccc agttgactgg tgacaggtgt
    20041 cagaaggagc caagagtagt ccccaatcca aggactgggc tggtgagtta atcgaatccc
    20101 ctgaaagata ggcagatgcg gatcaaaaaa ccaagatcca tcctggtcac tggtctgggg
    20161 cacaaaataa ttcatcccaa aaggcacgcc tgtgtatggc agggtatttc cccgagagaa
    20221 ggcatgtttg ctagcagtac caaagcgggt atcgatggtt tcaagtagtg gtttcatagt
    20281 ctttccttta gctgtttttc tacattatat cagaaaaaga gggcctttag aaaaggagtt
    20341 ttaaagataa ctattttgta gaaaaatgac tatcatttgt gtatgtattt tctgtctcaa
    20401 aagctatata ctgaaaatga aacttgtttg aaagaggtaa aatttaaaat ggtttattcg
    20461 aaagaaattg ttagagaatg gctagatgaa gtagcagagc gggccaagga ccatccagag
    20521 tgggtggatg tctttgagcg ttgctacacc gataccttgg acaatacggt tgaaatctta
    20581 gaagatggtt caacttttgt cttgactggg gatattcctg ccatgtggct tcgagattcg
    20641 acagcccaac tcagacccta ccttcatgta gctaaaagag atgccctcct gcgtcagacc
    20701 attgcaggtt tggtcaaacg tcagatgacc ttggtactca aggatcccta tgctaactcc
    20761 ttcaacattg aggagaactg gaaagggcac cacgagactg accacacaga ccttaacggc
    20821 tggatctggg agcgcaagta tgaggtggat tcgctttgct atcctttgca gttggcttat
    20881 ctcctctgga aagagactgg cgagactagt cagtttgatg agatttttgt cgcagcgact
    20941 aaggaaattc tccatctatg gacagttgaa caagaccaca agaactctcc ttatcgtttt
    21001 gtccgagata cggaccgtaa ggaagatacc ttggtaaatg atggctttgg acctgacttt
    21061 gcagtgacag gtatgacttg gtcagccttt cgtccgagtg atgactgttg ccagtatagt
    21121 tacttgattc cgtcaaatat gtttgctgta gtagtcttgg gttatgtgca agaaatcttc
    21181 gcagcattaa acctagctga tagccagagt gttattgctg atgccaagcg tcttcaggat
    21241 gaaatccaag aaggaatcga aaactacgct tacaccatca acagcaaggg cgaaaagatt
    21301 tacgcttttg aagtggatgg cctaggaaat gccagcatca tggatgatcc aaatgtacca
    21361 agtctactag ctgcgcccta tctgggctac tgttcggtcg atgatgaagt gtatcaagct
    21421 actcgtcgta ccattctgag ctctgaaaat ccatacttct accaaggaga atacgctagc
    21481 ggtctcggaa gttctcatac cttctatcgc tatatctggc caatcgccct ttctatccaa
    21541 ggattgacaa caagagataa ggcagagaaa aaattcttgc tggatcagct ggttgcctgc
    21601 gatggtggta caggtgtcat gcacgaaagc ttccatgtag atgatccgac cctctactct
    21661 cgtgaatggt tctcctgggc taacatgatg ttctgtgagt tggtcttgga ttacttggat
    21721 attcgctaag gggctcgctt tagctcaacc gattcttatc agaatcacaa gtttacattt
    21781 aaaacgttaa aatttaaatt tagaatgagg ttttacttca tggaaaatgt tgttgtacat
    21841 attatctcac atagccactg ggatcgtgag tggtacttgc cttttgaaag ccatcgtatg
    21901 caattggtgg aattgtttga caatctcttt gatctctttg aaaatgaccc tgagttcaag
    21961 agtttccact tggatggaca aactattgtc cttgacgact acttacaaat tcgccctgaa
    22021 aatcgcgaca aggtccaacg ctacattgac gagggcaagc ttaaaattgg tcccttttac
    22081 atcttgcagg atgattattt gatctccagt gaagccaatg tccgcaatac cttgattggt
    22141 caacaagaag ctgccaaatg gggtaaatca acccagattg gctactttcc agataccttt
    22201 ggaaatatgg gacaagcgcc tcaaattctt caaaaatcag gcattcacgt ggcggccttt
    22261 ggtcgtggtg tgaagccgat tggatttgac aaccaagtcc ttgaagatga gcagtttacg
    22321 tctcagtttt cagaaatgta ctggcagggt gtggatggta gtcgtgtttt aggtattctc
    22381 tttgccaact ggtacagtaa cgggaatgaa attccagttg acaaagatga ggccttgacc
    22441 ttctggaaac aaaaattgtc agatgtgcgt gcctacgctt cgaccaacca atggttgatg
    22501 atgaacggct gtgaccacca gcctgtccag aaaaatctga gcgaagccat tcgtgtggca
    22561 aatgaactct tcccggatgt aatctttgtt catagttctt ttgatgaata tgttcaagct
    22621 gtagaaggtg cgcttcctga acacttatca actgttacag gtgagttgac cagtcaggaa
    22681 acagatggct ggtacacact tgccaacact tcttcatccc gcatttacct aaaacaagcc
    22741 ttccaagaaa atagcaacct cctagagcaa gtggtagaac ccttgactat tatcactggt
    22801 ggacacaacc acaaggacca gttgacctat gcttggaaaa cacttttgca gaatgcgcca
    22861 catgatagta tctgtggctg tagcgtggac gaagttcacc gcgagatgga aacgcgtttt
    22921 gccaaggtca accaagtagg aaactttgtt aaaagtaact tgctcaacga gtggaagggt
    22981 aaaattgcta cggataaggc tcaaagtgac tatctcttta ctgtcattaa cacaggcttg
    23041 catgataagg tcgatactgt cagcacagtg attgatgtgg cgacttgtga tttcaaggaa
    23101 ttgcacccaa cagaaggcta caaaaagatg gctgctctta tcttgccaag ttaccgtgtg
    23161 gaggacttgg atggtcgtcc tgtagaggct acaatcgaag acctcggagc taattttgag
    23221 tatgatttac caaaagacaa gttccgccaa gctcgtattg ctcgtcaagt gcgcgtgacc
    23281 attccagttc acctagcgcc gctttcttgg acaaccttcc aattgctgga aggaaaacaa
    23341 gaacaccgtg agggtattta ccaaaacgga gtgattgata caccattcgt aacggtgagt
    23401 gtggatgaca acatcacagt ctatgacaag acaactcacg aagcctatga agactttatc
    23461 cgctttgaag accgtgggga catcggaaac gagtatatct atttccaacc aaaaggaaca
    23521 gagccaatct ttgcagagct taagggccac gaggtcttgg aaaacacagc tcgctacgct
    23581 aaaatcttgc tcaaacatga attgaccgtg cctgtcagtg cagatgaaaa gctagaagaa
    23641 gagcaaaaag gtatcatcga gttcatgaag cgtgaagctg gacggtcaga agaattgaca
    23701 aacattcctc tggaaactga gttgactgtc tttgttgaca atccacaaat ccgcttcaag
    23761 actcgcttta ctaacactgc caaggatcac cgtatccgtc tcttggtcaa gactcataac
    23821 acgcgtccaa gcaatgattc tgaaagtatc tatgaggtgg tgacacgacc aaacaaacca
    23881 gctgcttcat gggaaaaccc tgaaaatcct caacaccaac aagcttttgt cagtctgtat
    23941 gacgatgaaa aaggggtgac tgtatccaat aagggattga atgaatacga aatccttggg
    24001 gataacacca ttgccgtgac cattttgcgt gcatcaggtg agctaggtga ctggggctac
    24061 ttcccaacgc cagaagcaca atgcttgcgt gagtttgaag tcgagtttgc acttgaatgc
    24121 caccaagccc aagaacgctt ctcagcctat cgtcgtgcca aagccttgca gacaccgttt
    24181 accagccttc agcttgctag acaggaagga atcgtggttg cgactggtag cctcttgagc
    24241 cattctgttc tcagcatacc gcaagtttgt ccaacagcct ttaaagtggc ggaaaatgaa
    24301 gaaggctatg tcctccgtta ctataatatg agtcaagaaa atgtgcgcat atcagaacat
    24361 caacaaacca ttcttgactt acttgagcga ccatatccag ttcattcagg actattggat
    24421 ccacaagaga ttcgtacaga attcatcaaa aaagaagaaa tttaatttca aaaagtaaac
    24481 atcaaaagaa aggaggggcg aaaaagtaag aactaactgc tgattcgccc ctttttatgg
    24541 taaaaacaat gaccattgca acgattgata tcggagggac tgggattaag tttgccagtc
    24601 tgactcctga tgggaaaata ctggataaga caagtatttc aacgcctgaa aacttggagg
    24661 atttactagc gtggctagat caacgcttgt cagaacagga ttacagtggg attgctatga
    24721 gcgttccagg tgcagtcaat caagagacag gtgtgattga tggcttcagt gcggtgccct
    24781 acatccatgg cttttcttgg tatgaggcgc ttagctctta tcagctacct gtccatttag
    24841 aaaatgatgc caactgcgtt ggactcagtg aactactagc tcattcagag cttgaaaatg
    24901 cagcctgtgt cgtgattggg acagggattg gcggagccat gattatcaat ggtagacttc
    24961 atcgaggtcg ccacggcttg ggtggagaat ttggctacat gacaaccctt gcccctgctg
    25021 aaaaacttaa taactggtcg caactagcgt caactgggaa tatggtacga tacgtgattg
    25081 aaaaatctgg tcatactgat tgggacggtc gcaagattta ccaagaggcc gcagctggta
    25141 atgctctttg tcaagaagcc attgagcgca tgaaccgcaa tctggcgcaa ggcttgctca
    25201 atatccagta tctcatcgat ccagatgtca tcagtctggg tggctctatc agtcaaaatc
    25261 cagattttat ccaagatgtc aagaaggctg ttgataactt tgtcgatacc tacgaagaat
    25321 acacggtcgc accagttatc caggcctgca cctatcacgc agatgccaat ctctacggtg
    25381 ctcttgtcaa ctggctacag gaggaaaagc aatggtaaga tttacaggac ttagtcccaa
    25441 acaaacgcaa gctattgagg ttttaaaagg tcacatttct ctaccagatg tggaagtggc
    25501 tgtcactcag tctgaccaag cctctatctc tatcgagggt gaggaaggtc actatcaatt
    25561 gacctaccgc aaacctcacc aactctatcg tgccttgtcc ttgttggtaa cagttctagc
    25621 agaagctgat aaagtagaga ttgaggaaca agcagcttac gaagatttgg cttacatggt
    25681 tgactgttct cgaaatgcgg tgctgaatgt ggcttctgcc aagcagatga ttgaggtctt
    25741 ggctctcatg ggctactcaa cctttgagct ttacatggaa gacacttacc agattgaagg
    25801 gcagccttac tttggctatt tccgtggagc ttattcagca gaggagttgc aggaaatcga
    25861 agcctatgct caacagtttg acatgacctt tgtaccatgc atccagacct tggcccactt
    25921 gtcggccttt gtcaaatggg gtgtcaagga agtgcaggag ctccgtgatg tagaggacat
    25981 tcttctcatt ggcgaagaaa aggtttatga cctgattgat ggcatgtttg ccacgttgtc
    26041 taaactgaag actcgcaagg tcaatatcgg gatggacgaa gcccacttgg ttggtttggg
    26101 acgctacctg attttgaacg gtgttgtgga tcgtagtctc ctcatgtgcc aacacttgga
    26161 gcgcgtgctg gatattgctg acaaatatgg tttccactgc cagatgtgga gtgatatgtt
    26221 cttcaaactc atgtcagcgg atggccagta cgaccgtgat gtggaaattc cagaggaaac
    26281 tcgtgtctac ctagaccgtc tcaaagaccg tgtgactctg gtttactggg attattatca
    26341 ggatagcgag gaaaaataca accgtaattt ccgcaatcat cacaagatta gccatgacct
    26401 tgcatttgca gggggagctt ggaagtggat tggctttaca cctcacaacc attttagccg
    26461 tctagtggct atcgaggcta ataaagcctg ccgtgccaat cagattaaag aagtcatcgt
    26521 aacgggttgg ggagacaatg gtggtgaaac tgcccagttc tctatcctac caagcttgca
    26581 aatctgggca gaactcagct atcgcaatga cctagatagt ttgtctgcgc atttcaagac
    26641 caatactggt ctaacggttg aggattttat gcagattgat cttgccaacc tcttaccaga
    26701 cctaccaggt aatctcagtg gcatcaatcc caaccgctat gtcttttatc aggatgttct
    26761 ctgtccgata cttgaccgac acatgacacc tgaacaggac aagccacact tcgctcaggc
    26821 ggcgggaact attgctgaca ttaaagaaaa agctggaaac tatgcttatc tctttgaaac
    26881 tcaggcccag ttgaatgcta ttttaagtag caaagtagat gtgggacgac gcattcgtca
    26941 tgcctaccaa gcggatgata aagaaagttt acaacaaatc gccagacaag aattaccaga
    27001 acttagaagc caaattgaag acttccatgc cctctttagt caccaatggc tgaaagaaaa
    27061 caaggtcttt ggtttggata cagttgacat ccgtatgggc ggactcttgc aacgcatcaa
    27121 acgagcagaa agccgtatcg aggtttatct ggctggccag cttgaccgta tcgacgagct
    27181 ggaagttgaa atcctaccat ttactgactt ctacgcagac aaggatttcg cagcaactac
    27241 agctaaccag tggcatacca ttgcgacagc ttcgacgatt tatacgactt aatactcttc
    27301 gaaaatctct tcaaaccacg tcagcttcca tctacaacct caaaacagtg ttttgagcag
    27361 cctgcagcta gcttcctagt ttgctctttg attttcattg agtatgaaaa caagaacacc
    27421 ttgcttggcg cagggtgttt cgcgtgaaac agaagaatta tctggtttca aatgctacag
    27481 ttagacaaac ttatgataaa gtcgggatta taacacagag ataagaagaa atagatagtg
    27541 ggacgagttt aaataggatt actcgctatg aaatgttaat tacttttgat ggcttaacaa
    27601 atattatcaa taatgtagtc attcgaatga tttcccttgc aatatagtga aattaacaaa
    27661 aagaaaaaac gctgatcaag cgtttttctt ctgtattgat tcgtattgaa tgcttactta
    27721 acttctgtaa acacaacgtg tttgcgaagt tttggtgagt atttcttcaa ttgaaggcgg
    27781 tctggagtgt tacgtttgtt tttagaagta aggtacaagc gttcaccaga ttctttgtgt
    27841 tcaagtgtaa tatttacgcg catggtatct cccttctatt attcagctga tgcagcttta
    27901 gcgattttac gtcctttgta gtatccttta agtgatacac ggtgagaacg tgagtaatct
    27961 ccagtagttt cgtcaaagtt tacagatgga gctgttactt tgtagtgtgt acgacgtttg
    28021 tttttcttcg cttttgaagt gcgacgtgca ggtactgcca ttttgatttc tcctttaggt
    28081 atttatattc gattcaatct actgattttc atcaaccata ctaggataac acattttttt
    28141 tgtaaagtaa agttacttga caaaaaaaga tgaaaaaatt ctcctatccg acacggcaga
    28201 caggagaagg tgttaaatat caatctcaaa tggttcgtca atggtttctg atacgtattt
    28261 tccgtctttt ttccgttgct tgacacactc tgtgaggaga tattcgattt gcccattgac
    28321 tgaacgaaag tcgtcttctg cccatgatgc gagtgcagcg tataactttg ttgagagtcg
    28381 aagggggatc tgcttttttt tagcttcagc catctttagt aaagacttcc tgtgttgaca
    28441 attggttgtg catcatgatt gccacaaaga acgacaagga gatttgaaac catggcagct
    28501 tttcgttctt cgtcaagttc taccaattcc ccttcattga gccgttctag tgccatttca
    28561 accattccta cagcaccatc tacaatcatc ttccgtgcat caataatggc agatgcttgt
    28621 tggcgttgaa gcataacggc agcaatttct ggagcataag ctaggtaagt gatacgtgct
    28681 tcaaggattt ccaagccagc atcttctacg cgactttgaa tttcctcacg aatacggtta
    28741 gcaacaattt cgctagagcc acggagacta ccttcatctg cttgcccgtc acccgtagta
    28801 tccacattag gagacacatc gtaaggatag atgcggacaa tattacggag ggcgctatca
    28861 cattgcaatg aaagatattc tttgtagtta tcaacgttga agactgcctt agcggtatcg
    28921 acaactctcc aagttaccgc gataccgatt tctacagggt ttcctaagca atcattgatt
    28981 ttttgacgag aattgctcaa ggtcatgact ttgagggaaa tgtgtttctt gccaatttca
    29041 agatttacat cattgtcatt tgatgattta gctcctaaaa aaggagattt tgtgctaaca
    29101 tcaccacttt gtccaagtcg agtgtggttt gcagggttga ctgctacgct gaagggattg
    29161 acaaagtaaa aaccaggttc tttcatggta cctgtataat taccaaagag tgtcagaacc
    29221 agagcttcct gaggtttgac aacttttaaa ccagcatgag ctagacctgc aattacgatt
    29281 agtaaaggtc cgataataat tccaaaaatg ttttccgaac caacacccat tataaatata
    29341 aagataccaa gtacgattgt caactcaatg agaatcaatg cgaatacacc atttggtttt
    29401 gaattgatta gtttttcagt cataagaatg acctcccatt atttgatatc taaatgatat
    29461 cactttgttt ttaaaagtca atagaaagtt gaaaataatc actaaaaact ttcagcccaa
    29521 taatttcttt taaaaggtga gattatattg tgctcaaata ctcttttatt cttataattt
    29581 ttacactata attgtagtta gaaaggaaga gaaatgtttg attatagagc actagttatt
    29641 ttgatgactt tgatggatca ttgtgagcta tcattatatg aattatctgt taaggtgagc
    29701 ttacctataa aggaagtcaa agaaggaata gattatttag tgccttatct agctaataaa
    29761 gggatagtgc tggataaaaa acaaggtcgc tatagtttat caaatcgtac gaagcagtct
    29821 ttgacagata ttattaagtc ggatgaattg gttttaccaa agtcgactcg cttagcttta
    29881 atctatcttt acactttttg ccgattagat tttatatcga ataatcatta ccaagacttt
    29941 ttgaaagtaa gtaagaatac aaccttatct gatattcaat cattaagaaa gattatgtta
    30001 gataatgatt tggagctagg gtacagtaga gcaaaaggtt atactttaca cggttcagag
    30061 tggaataagc accgcttagc ttttcaaatg gttagtgagt tgctggaatc ctccataggg
    30121 atttggggtt tggattatgt tttatccagt tgggggtatt cattaactta tgatttgata
    30181 aatcaggtag ttaaagatta ttatgagaag ctacagctag tacctattgt taatcaatta
    30241 aaagtttacc ttttcggttt agtattcatt ctgtgtaggt atcaaaggga tgttgaaaga
    30301 gtatgtcttt cagagacatt agtatctcct gttattcaag atataaccac tattttattg
    30361 gatacagtgg ttgatttggg aattatagat acggtgtttt ctgaggatga ttatcgctat
    30421 atcacagttt tattatcaag ttgctttgaa ggtgaagtag atgttgctcc ggtttacttt
    30481 aatcaattaa cagaggctat tataagtaga atggaagata tttctttgtt acattttaaa
    30541 caaagggaag gattgagaga aaatcttcgc cgccacctta taccggctta ctatagatta
    30601 aagttcggcc tacctagttc aaatgaatat gtattgcatg ttaaagaaca ttatcctgat
    30661 ttatttgaat tagtcaaaga ttctttgatg cccttgatgg acgctattga caatcccata
    30721 cctgatagtg aaacagcata ttttgttatc catttcggag gttatttaaa gaaagcagat
    30781 actttgcctc aaaaatgcta taaagcagca atcatttgtc ctaatggtgt tagttcttca
    30841 ttgatgttaa aagagaatct attagcatta tttcctcaaa ttgagtttat aggaacctca
    30901 aagattgatg atttacaggt gaaagttagt agtgactatg atatggtttt ttctaccata
    30961 aaggtggaga cagaaaagcc aaattatcta gtttcggtta tgatgactga agagcaagca
    31021 atacaactag tagaactagt gttaaaagat tttccgaatt tagagtatgg agattttgag
    31081 attgaacaaa tcctgaatat tgtcaaaaga tatggaatta ttacacaaga attggaatta
    31141 agattggcgt taaagaatta tctttatcaa aaaaatgata gaaaggaaat tgtaccagtg
    31201 ctagaacaac ttattaccaa agaaacctat caggttagtt cgcaaaaatt aggatggaag
    31261 gaggcgattc gtttagcagc taaaccgctt ttggatcaag ataagataac tgagaactac
    31321 cctgaggcaa tgattcaaaa agtagaagag tttggacctt ttattaattt agggaaggga
    31381 gtagcaattc ctcacgctcg gccagatgaa ggtgtgaatg aaataggaat gtcaatgtta
    31441 gttttggaag aaccgattta tttattggat aatccagaac aagaggtaag attgttgatt
    31501 tgtattgcag ccattgataa tgagagtcat ttaaaggctt tgtcacattt aacaacaatt
    31561 ttaagagata aaaatcatgt tcaaacttta atatcatcaa aaaactataa tgatattaaa
    31621 atgataatta aacaggagga ttagataatg ttaaaaattg gtacagcttg tggttcagga
    31681 ttaggttcaa gttttatggt acagatgaat attgaatctg tattgagtga tttgaatgtt
    31741 tcggatgtag aagttgaaca ttatgattta ggtggagcag atccaaatgc agctgatatt
    31801 tggattgttg gtcgtgatct agctgattca gctagtcatc ttggagatgt tcgtatctta
    31861 aatagtatta ttgatatgga cgaactacga gaattaatta ctaaaatttg tgaagaaaaa
    31921 ggacttatat aggaggctag cgtcatgatg aagttcatat tggatattgt tagtacacca
    31981 gctattttag tagctttaat tgcaatctta ggattagttc ttcagaagaa gaaattacct
    32041 gatattatta aaggtggaat taagaccttt gttggtttct tagttgtatc tggtggtgca
    32101 ggaattgtac aaaattcttt aaatccattt ggtaccatgt ttgagcatgc ttttcattta
    32161 tctggcgttg tgccgaataa tgaagcaatt gtagctgtag ctttaacaac atatggctca
    32221 gctactgcaa tgattatgtt tgcaggcatg gtgttcaata tcttaatcgc tcgttttact
    32281 cgatttaaat atattttttt aacagggcac cacactctat atatggcatg tatgattgcg
    32341 gtcattttat cagttgctgg ctttactagc ttgcctctca tcttactagg aggattagca
    32401 ctcggtatta ttatgagtat ttccccagca tttgttcaaa aatatatggt tcaattaact
    32461 ggaaatgaca aggtggcttt aggtcatttc agttctttgg gatattggtt gagtggtttt
    32521 actggtagcc ttatcggtga caaatcaaaa tcaacagagg acattaaatt tccaaagagt
    32581 ttagcttttt tacgtgatag tactgttagt attactttat ccatggcagt tatttacatt
    32641 attgtagcta tctttgcagg gtcagaatat atagaaaaag aaatcagtag tggtacaagt
    32701 ggtctagttt atgctttaca attagcaggt caatttgcag caggggtatt tgttatttta
    32761 gcaggtgttc gccttatttt gggcgaaatt gttccagcct ttaaaggtat ttcagagcgt
    32821 cttgtaccta attccaaacc tgctttggat tgtccgattg tttatactta tgcacccaat
    32881 gcagttctaa ttggatttat ctctagtttt gttggtggtt tagtaagtat ggtaattatg
    32941 attgcttcag gaacggttgt tatcttacca ggcgttgtac ctcatttctt ctgtggagcg
    33001 actgcaggtg tcattgggaa tgcatctggt ggtgttcgtg gagccactat tggagcattt
    33061 ttacaaggta ttttaatcag ttttcttcca gtctttttaa tgccagtttt gggaggactt
    33121 ggtttccaag gatcaacttt ctcagatgca gattttggtc tatcaggaat tattttagga
    33181 atgttaaatc aatttggttc acaagcaggc attgtgattg gtcttgttct tattctagca
    33241 gttatgtttg gagtatcctt tattaaaaag ccatctgcaa aggaggaata agggatgatt
    33301 ttaagtaaaa atagagaaga tgggttaaga aaatttgcga ctaacatccg attaaatact
    33361 cttagaacat tgaatcatct tggattcgga cattacggag gaagtctgtc tatcgtagaa
    33421 gttttagcag tgctttatgg tgaaataatg ccaatgactc cagaaatatt tgcagcacga
    33481 gatagagatt atttcatatt atcaaaaggt catggaggac cagctctata cagtacactc
    33541 tatttgaatg gtttctttga caaagaattc ctatattctt taaatacaaa tggaaccaaa
    33601 ttaccgtctc atcctgatag aaatctaacg ccaggcatag atatgacaac gggctcttta
    33661 ggacaaggaa ttagtgttgc aactggactt gcatatggtc agagaataag aaagagtccc
    33721 ttttatactt atgctattgt tggagatggt gagttaaatg agggacaatg ttgggaggct
    33781 atacagtttg cttctcatca acagttatcc aacttaattg tatttgttga tgataacaaa
    33841 aaacaattag atggttttac aaaggatatt tgtaatccag gtgatttcgt agaaaaattt
    33901 tcagcatttg gatttgaatc cattagggtc aagggttcag atattagaga aatttatgaa
    33961 gggattgtcc aattaaaaca gtcaaataat tcatcaccta agtgcattgt attagatact
    34021 attaaaggtc aaggggttca agagctggaa gaaatgaaat ccaaccatca tcttcgccct
    34081 actgtagagg agagacaaat gttaacttca gttgtagaaa gattaagtca ggaattggag
    34141 gaaacagaat gatgagaagc acgaaagaat tacggcatgt atatagagac ttccttttag
    34201 aggctaatca aagttattct gatatagtag tcttagaagc cgatttgtca agttcgatgg
    34261 ctactaataa tcttgaaaag gactttggag accgttatgt gaatgttggg attatggaag
    34321 cagaaatggt cggccttgca gcaggcttgt ctattcaggg gtttagacct tatcttcata
    34381 catttggtcc ttttgcttca cgaagagtat ttgatcaatt atttatttct cttggatacg
    34441 cacaattgga tgccactgtg attggatcag atgcaggagt aactgcagaa atgaatggtg
    34501 ggacacatat gccatttgaa gaaattggat tgttacgttt aattcctaaa tcgatcattt
    34561 ttgaagcaac tgatgatatc caatttcgtg aaatattgaa ccagacatta gacttaaaag
    34621 gactaaaata tattcgaaca attagaaaag ctccagttcc tgtgtatcaa ggtggagaag
    34681 atttttctaa aggctacatt gagttaaggc atggtgaaga tcttgtaatc gttgcttctg
    34741 gtataatggt tgctccaagt attcgagttg cagatgaact gtctaaatta ggttattcag
    34801 taggtgtgat agatttattt agaatcaaac cgataccaga acagataaaa acaatgttaa
    34861 gtggaaaaac tatatttact gtagaaaacc acaatcagat aggtggaatt ggcagtgctt
    34921 tatgtgaatt attctctgat gatggaataa caaaaattca tcggatgggt gttcaagaaa
    34981 gatttggtca agtagggaaa atggattatc ttcttaatga gtatggtttg agtgaatcga
    35041 atataaaaga gaaggttcta gaaatttata atgcaagata gatgttaaat acacttatct
    35101 gaaagaattt ctgtaaaaat tacattacgt tatttaaaga aagcggatta aaaagcatat
    35161 tctggtcatc agaaagtgca ctaatccgct ttgttgtttt taattttaaa tatttcacat
    35221 gcgtatagaa ttttctgaaa attcaagaat tttattctgt tcattgcctt taaattgtgc
    35281 tataatagta aaaactgaaa tgggagggat aagatgactg aattagataa acgtcaccgc
    35341 agtagcatct atgacagcat ggttaaatca cctaaccgtg ctatgcttcg tgcgactggt
    35401 atgacagata aggactttga aacatcgatt gtgggagtga tttcgacttg ggcggaaaat
    35461 acaccatgta acattcactt gcatgatttc gggaaactgg ctaaagaagg tgtcaaatct
    35521 gcaggcgctt ggcctgtaca gtttggaacc attaccgtag cggacgggat cgctatggga
    35581 acgcctggta tgcgtttctc tctaacatct cgtgacatca tcgcggactc catcgaggcg
    35641 gctatgagtg gtcacaacgt ggatgccttc gtcgctatcg gtggctgtga caagaacatg
    35701 cctggatcta tgattgctat tgctaatatg gatatcccag ctattttcgc ctatggtgga
    35761 actattgcac cgggaaatct tgatggtaaa gatatcgact tggtttctgt ctttgaaggt
    35821 atcggaaaat ggaaccacgg tgacatgaca gctgaggacg tgaaacgtct tgaatgtaat
    35881 gcctgccctg gccctggtgg ttgtggtggt atgtatactg ctaataccat ggcaactgct
    35941 atcgaagttc tagggatgag tttgccaggg tcatcctctc acccagctga atcagctgat
    36001 aagaaagaag atatcgaagc agcaggacgt gctgttgtta agatgttgga acttggtctc
    36061 aaaccatcag atatcttgac tcgtgaagcc tttgaagatg ctattactgt aacgatggct
    36121 ctcggtggtt ctacaaacgc cactcttcac ttgctcgcca ttgcccatgc cgcaaatgtt
    36181 gacttgtcac ttgaggactt caatacgatt caagaacgtg tgcctcactt ggccgacttg
    36241 aaaccatctg gtcagtatgt cttccaagac ctctacgaag tcggtggtgt ccctgcggtt
    36301 atgaagtact tgttggcaaa tggtttcctt cacggagatc gcatcacatg tactggtaag
    36361 actgtagctg aaaacttggc tgactttgca gacttgactc caggccaaaa agttatcatg
    36421 ccacttgaaa atccaaaacg tgcggatggt ccgcttatca tcttgaacgg gaaccttgct
    36481 cctgacggtg cagttgccaa ggtatcaggt gttaaagtgc gtcgtcacgt tgggccagct
    36541 aaggtctttg actcagaaga agatgcgatt caggccgttc tgacagatga aatcgttgat
    36601 ggcgatgtag tcgttgttcg ttttgttgga cctaaaggtg gtcctggtat gcctgagatg
    36661 ctgtcacttt catcaatgat tgttggtaaa ggtcagggag ataaggtggc cctcttgacg
    36721 gacggacgtt tctctggtgg tacttatggt ctggttgttg gacatatcgc tcctgaagct
    36781 caggatggtg gaccaattgc ctatctccgt accggcgata tcgttacggt tgaccaagat
    36841 accaaagaaa tttccatggc cgtatccgaa gaagaacttg aaaaacgcaa ggcagaaaca
    36901 accttgccac cactttacag ccgtggtgtc ctcggtaaat atgcccacat cgtatcatct
    36961 gcttcacgcg gagccgtgac agacttctgg aatatggaca agtcaggtaa aaaataaact
    37021 catactcgaa aatctcttca aatcacgtca acgtcgcctt gccgtaggta tggttactga
    37081 cttcgtcagt tctatccaca acctcaaaac agtgttttga gctgacttcg tcagttctat
    37141 ccataacttc aaaacagtgt tttgagcaac ctgcggctag ttttctagtt tgccctttga
    37201 ttttcattga gtatcaaaaa gcaagccatt ttcggcttgc ttttttcatc tttaaaagtg
    37261 attggcctgc ttaacgaggt ggtagtccaa gggcaatacg gccatagcga ctgattcgtg
    37321 tgattttcca ggcaggcgac caggtaactt caaccttgat atctttgata ccctcgattt
    37381 gtttcagacc tgccacgatt tcaataggca ggctttcggc gcaatcacag gcagtatcgg
    37441 tgaaggtcat gacaatcttg cagagccccg tttcatccag attgatttca taaatcagac
    37501 ctagattgta aacatccaat tccacatctg tatcaaaaac tttctctagt ttttcgataa
    37561 tttggtcttg caaggccaaa gcacggtcat tgattttgat atcgtctctc atagcagtcc
    37621 ctccacgtga taaatatcct ctattttctc ataattctga caatttggca agtttttgat
    37681 gaattttctg aaaaatatga taaaatgaag aaaatacgga atcaagggga agtctatgga
    37741 gcagattgga aaagtcttta gacaattacg agaatcaaga aatatctcac tgagacaagc
    37801 aaccggagga caattttcgc cgtctatgtt gtcccgtttt gaaacaggtc agagtgagct
    37861 ttcggtggaa aagtttctat ttgctctgga aaatatatct gccagtgtgg aggagatcct
    37921 ctttctggcg agaggtttcc agtatgatac agattctgag ttgagaaaag aaattacaga
    37981 tgtcttggaa ccaaagaata tagcacctct tgaggactta tatcgcaggg agtatcaaaa
    38041 gcatgcccat tctcacaaca aacagaaaca tattctaaat gccattatga tcaagtctta
    38101 tatgaagagc atggatgaaa gggtagagtt aacagcagag gaaggaaaag tccttcatga
    38161 ttatttgttt tctaccgaga tctggggaat ctatgaactc aatttatttt cagtcagttc
    38221 tgccttttta tctgtttctc tttttactag atatgtacga gaaatggtac gtaaatctga
    38281 ttttctaatg gaaatgtctg gtaatcgaaa cttttttcat actatactat tgaatggctt
    38341 tttagctagt attgagtgtg aagaatttac caatgcctat tattttaaac gtgttatcga
    38401 agagcatttc tacaaggaaa atgagaccta tttccgaatt gtttatctgt gggcagaagg
    38461 tctccttgat agcaagcaag gtagagtcaa ggaaggccag aaaaagatgg aggatgctgt
    38521 ccgtattttt gagatgcttg gctgtaataa atctgccgaa tactatagaa atacgaccga
    38581 atgttgaata tctgcaaacc aagaaaaaat ttgaagcgat agaactattg ccctctctca
    38641 ggtcattaca aaagttctat cgtttctttt tgctcatttt atttgttgca tatattcaag
    38701 agttctccct ctcaaatttc taagtgctat actatagtca tacttcatat agaggttcga
    38761 acaagaaggg agattatcta tgaaactatt gtttagaaat ccagcttata gaattttgac
    38821 tttatcatgc ttcttcaatg cttttggtgc ttcgattttc aacctggtct ttatcgtcta
    38881 tgcatcgacc ttgtcacaag catcttttgc tgttgctatg gcgaatattg tcatgattct
    38941 tccgaccctc tttacagttt ttgtagggat tcgggcagat tacacgaggg acaaggtcaa
    39001 atggatggtc tatagcggtt tgtttcaggc ggttctattt tttctagcag ccctagttgt
    39061 tcagcaagct agtctctttg ccttttctag cctgtgtttg atcaatgtca ttagtgatat
    39121 catcagtgat tttgcaggtg gcctgcgtat gcctcttatt aaggaaaaag tagctgaaga
    39181 tgatctgatg gaggcttatt ctttttcaca gttcatcacc tatatttcag ctattggtgg
    39241 tcaagctttc ggagtctggc tcttagctct atcggtcaac aatttttctc tcgttgcggg
    39301 aatcaatgcc tgctttttcc tagtatcagc cactatcctc tttttaggaa aaagcaaatt
    39361 gagcctgtca atgtcatctg ctgatggtga aaacttaaaa aatgagaagc tttctatcaa
    39421 agaccagttc ctaacaattt accgaaattt acgtctcgtt tttcttaaaa gtggacagaa
    39481 aaactttggt tttatgctct ttgctgtctt gcttattaat tccttgggtg gcgctttggg
    39541 tggaatttat aatatcttct ttttgagcca ttctctcttg aatttttctt atacagaggc
    39601 actatttatc aatcaattct gtgttttagt agcagtcatc atcagtagcc ttacgggcaa
    39661 tgattatttt gggaagcagt ccttgcctag attgatgatg tgggcgaccg taggacttag
    39721 tctagttggt ttggctaatt tattcaatca agtcgtgctt ggtttgctat ttctcttctt
    39781 taccctgtat gtgtctggta aagttcaacc aaagattagt gccatgctca tgaaaaatct
    39841 agctccagag gttctagctc gtactagtaa ttttttaggt ctattgttta ccttatccat
    39901 acctgtggga acagcttgtt tttcactagt agccgtatgg aatatacagt tgacttggat
    39961 gctatttgtt ggtctttcct tgctagctat ttttttgaca attcttaatc tcaaaaatga
    40021 tatctaaatc ctaatttttt tcttactgtt ttaatccctt tatttgtggt aaaataagac
    40081 tattaagttt aaagaggatt ccctatgaaa ttacaaaaac caaaaggaac gcaggatatt
    40141 ttacctgctg aatctgctaa gtggcagtac gttgagggct ttgcccgtga gattttcaag
    40201 cgctataact atgcagaagt gcgcacgcct atttttgagc attacgaggt tatcagtcgc
    40261 tctgtcggag atacaacgga tatcgtaacc aaggaaatgt acgattttta tgacaagggt
    40321 gaccgtcata ttaccctccg tccagaagga actgcgcccg ttgtccgttc ctatgtggaa
    40381 aataaactct tcgccccaga agtgcaaaag ccaagcaagt tctactacat gggacctatg
    40441 ttccgttatg agcgtccaca ggcagggcgc ttgcgccaat tccaccagat tggtgttgag
    40501 tgttttggtt ctagcaatcc agctaccgat gtggaaacaa tcgttatggc agcccatttt
    40561 ttgaaggaaa tcggtattca aggtgtcaaa ttgcacctca acactcttgg aaatcctgag
    40621 agccgtgcag cctaccgcca agccttgatt gactatttga caccgctcaa ggagaccttg
    40681 tctaaggata gccaacgtcg cttggaggaa aatcctcttc gtgtcttgga ctctaaggaa
    40741 aaagaagaca aggtggcagt agagaatgcg ccgtctatct tggactttct tgatgaagaa
    40801 agccaaactc attttgatgc tgtgagtcag atgttggaaa atcttggagt agattacatc
    40861 atcgatacca atatggtgcg tggtctggac tactacaacc acaccatttt cgagtttatc
    40921 acagagattg agggcaatga cttgacagtc tgtgcgggtg gtcgctacga tggtttggtt
    40981 gcttactttg gaggccctga aactgctgga tttggttttg ggcttggtgt agagcgcctg
    41041 cttctcatcc ttgaaaaaca aggcgtggcc ctccctatcg aaaatgccct agatgtctat
    41101 atcgcagtct tgggtgatgg agcaaatgtc aaagccctag aactagtcca agcccttcgc
    41161 caacaaggtt tcaaagcaga gcgtgattac ctcaaccgta aactcaaagc tcaattcaag
    41221 tcagccgatg tctttgcagc taagaccctc atcaccctag gagagagcga agtcgaaagc
    41281 agacaagtga cagtcaagaa caaccaaacc cgagaagaag tgcaagtgtc acttgagaca
    41341 atcagccaaa acttctcaga aatctttgaa aaactaggat tttatactca atgaaaatca
    41401 aagagcaaac taggaagcta gccgcaggtt gctcaaaaca ctgttttgag gttgtggata
    41461 gaactgacga agtcagctca aaacatggtt ttgaggttgt agatgaaact gacgaagtca
    41521 gtaaccatac ctacggcaag gtgaagctga cgtggtttga agagatttta gaagagtatt
    41581 aatgatagat aaagtggtca ggaacttact cctgaccttt ttcttttgca aaatgacaaa
    41641 aatcataaac tttttgacaa atatatttat caaaaagaaa aagatgtgtt acaatgaatt
    41701 caagttaaga aataggaagc agaaaggagt tataatgtgg gtactagtat ttatactatt
    41761 tatgattttc ttttattcta ataattctaa aaaaatcaag aaactagaga ataaaatcaa
    41821 aaaacttgag cgaaaagaga aaggaaacgc agaaatgtcg agattattac aagaaatgat
    41881 tggaaaggaa ccaattataa cgggagtgta tattgggcca gataactggg aagttgtgga
    41941 tgttgatgag gaatgggtca aactacgtag tgttgataaa aaggggaaag aaaaattcaa
    42001 gttgcaacgt attgaagata tccaaaccgt tgaatttgac ggagagtagg tgtgtaacat
    42061 gcaactaaaa aatcgtctaa aagagcttcg agctcgcgat ggtctcaatc aaaccgacct
    42121 agccaaactg gcaggggttt ccagacagac cattagccta ctagaacggg atgagtacac
    42181 cccatccatt atcattgccc tgaaaatatc tcaaattttc aacgaaacag tcgaatcggt
    42241 atttcgtttg gaggaggacg agtgatgaac aagtataaag tgatttatta tgtagtggtc
    42301 atagctttat tagtcagtgt atttctactg attgggatgg acctaagctg gtttaatccc
    42361 tatcaaagcg accaatttgt ttgggtctac tttgctctca tcccagtaat tgaatggatt
    42421 gaaaagaaat ccaaaaatct agcaagtgaa aaaggagaat gaatatgaaa aagaaacgta
    42481 gattgttatt tttgatgtct attgtcttgg gggggttctt gggcatgttt gtagggatgt
    42541 ttaaggcacg tgtcgaatcc cacgaaatta ttttagatgt aaaagccttg atgccatgga
    42601 tatcagctat ttgtttactg ataggtttca ttagtatgtt tttgactttc aatttcttaa
    42661 agaaaagcag aaaatttcat tccttgtatc aagaggaaat ggatgacgat ctgaatgaaa
    42721 cctattatgt gcaaatgtat cggaatcttg agtttggaac cattgctttt aatattacag
    42781 gtgtagcgat tccattggct atttttattt cattaagtga ggtgattata ttgcatacaa
    42841 accctcaaac atttttcctt tctttcttac tctttgtggt attcttagtc gctcaaaaat
    42901 ctctttttaa aaccattgcg attgttcgtc agtttgattt ggaatttttc gctacaccaa
    42961 aggatgtctt gaactatata aattcttatg atgaagggga gcgtcaggct aatttggaac
    43021 agagttttcg aattttattc caattacacc aatatgtctt accagcctta tatatttttc
    43081 ttattatcat ttctttcttg acaggagaga ttcagttact agctttcttg cttgtaggag
    43141 ccatccatgt ttatatcaat gtgatgcagt tacctatggt aaaacgttat ttcaaataaa
    43201 ggagttatct atgaaactac ttaaaaacct tggctggttt cttctagccc ttctatcctt
    43261 tttctttatc tatggcttta ttcaggggct cgcgactgcg tcgcttgctt taggcgcttc
    43321 accctatgct gttactctac tctatgtggt cttggctgga gtatatgtgt acggcattta
    43381 caaatggtat cagaaagctc ctgtttatat tgagaagagc ggctttaacc gatttatttg
    43441 gcttcctgcc ttggtttggt tcttatctct ggtcgttcaa ttcttcttgc cagatgatcc
    43501 ttcagtaaat caacaaatag cgacagactt gaccttgtct caaccacttt tctcattctt
    43561 tgctgtggtt atttttgctc ctttgacgga agagattgtt tttagaggga tgttagcacg
    43621 ctatctcttt cctaagcagg acaatagtaa acgaaccctg atttttcttc tggtatctag
    43681 tcttctattt gccttgattc attttccagg tgatgtgcaa caattttttg tctattttag
    43741 ccttggtttt agtttgggtt tggcttacat tagcagaaaa ggtctggtct acagtatttc
    43801 tcttcacgct ttgaataatt tagtcggctt tttgatgatt ctcatgctat aatagagtca
    43861 ggaggtcaca tgaaacgagt aattttatta gcagtgattc aggcagtcgt tctatttttc
    43921 atcattggag cgctagctta tgccttcaaa ggcgatttct tctataacta tctagcagtt
    43981 gtctttgctc ctattgcagg tgtactgcgt tttgggacgg cttacataac ggaaattgtc
    44041 ttgcctcgaa aggcagccga aatcgctgaa aagcgtaaag caggcaaaaa ttcaaaataa
    44101 aagagtccgg tgaagttcat cggatttttg tattatactc aatgaaaatc aaagagcaaa
    44161 ctaggaagct agccgcaggt tgctcaaaac actgttttga ggttgtggat gaaactgacg
    44221 aagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc agtaaccata
    44281 cctacggtaa ggcgacgctg acgtggtttg aagagatttt cgaagagcat tagcgctaat
    44341 ttttagggct ttcacctgac ttttaaagac tggcaaactt ttctgatgat tttcatgaag
    44401 agcctgcgct tttatggtaa aatagtaaca gaataaaaga ggagagaaat aatgaaacgt
    44461 agtatgtatg ctggtcgtgt tcgtgaggaa cacatcggac aagaaataac cttgaaagga
    44521 tgggttggcc gtcgtcgtga ccttggtggt ttgatcttta tcgatcttcg tgaccgtgaa
    44581 ggaatcatgc agttggttat caaccctgaa aaagtatctg cagaggttat ggcaacagct
    44641 gaaagccttc gtagcgaatt tgttattgag gtgactggtc aggtcgctgc gcgtgagcaa
    44701 gccaatgata agttgccaac tggtgcggtt gagttaaacg tgacagctct tattgtgctt
    44761 aacacagcta agacaacacc atttgagatt aaggatggca ttgaggcaaa tgacgataca
    44821 cgtttgcgtt accgttacct tgaccttcgt cgtccagaaa tgttggaaaa tcttaaactt
    44881 cgtgccaagg tgacccactc tatccgcaac tacttagatg agttggagtt tatcgacgtg
    44941 gagacaccat tcctttctaa gtcaacgcct gaaggggcgc gtgattattt agtgccgtct
    45001 cgtgttaata aggggcattt ttacgctctt cctcaaagtc cacaaatcac gaaacagctc
    45061 ttgatgaatg ctggttttga ccgttactac caaatcgtta aatgtttccg tgacgaggac
    45121 ttgcgtggag accgccagcc tgaatttact caggtcgact tggaaacgtc cttccttact
    45181 gagcaagaaa tccaagatat tacagaaggc ttgatcgcgc gcgtgatgaa ggaaacaaaa
    45241 ggcatcgaag taacgctacc gttccctcgt atgaaatacg atgatgctat ggctctttac
    45301 ggttctgaca agccagatac tcgttttgac atgttgcttc aggacttgac agaagtggtc
    45361 aaaggtgtag actttaaagt cttttcagaa gcacttgctg taaaagcgat tgtggtcaaa
    45421 ggagctgcgg acaactattc acgtaaagac atcgacaaga tgacggaagt agccaaacag
    45481 tatggtgcca aaggtcttgc ttgggtcaag gtggttgatg gagaattaaa cggaccagtt
    45541 gccaagttct tgactggtat ccaagaagaa ttgacaacag cgcttgctct tgaagataag
    45601 gacttggttc tctttgtggc ggatacgctt gaagtggcta atgcaacact gggtgccctt
    45661 cgtggacgta ttgctaaaga gcttggcttg attgataatg ataagttcaa cttcctttgg
    45721 gtggttgact ggccgatgtt tgaatggtct gaagaagaag gccgctacat gagcgcccac
    45781 catccattca cacttccaca ggaagagact gctcacgaat tagaaggtga tttggctaag
    45841 gttcgtgcca ttgcttacga tatcgtcttg aacggttatg agcttggtgg tggtagcctt
    45901 cgtatcaacc aaaaagacct tcaagaacgc atgttcaagg ctcttggttt ctcagctgaa
    45961 gaagccaatg accagtttgg tttccttctt gaagccatgg actatggttt cccaccacac
    46021 ggtggtttgg ctatcgggct tgaccgtttt gtcatgttgc ttgctggaga agaaaatatc
    46081 cgtgaagtca ttgcctttcc taagaacaac aaggcaactg atccaatgac acaagctcca
    46141 tcaacagtcg ctctcaaaca actagaggaa ctcagcttac aagtagaaga agatgaaaca
    46201 aacaaaacga attaagcggt ggcgctatta tctgcgccgc tttgcttatc agataaaaat
    46261 tttacgtgtc ttacaaagta tctctcgaga aaagtatgat gagaagattt cggcctctct
    46321 ggtctatggt tttttatcag cagtagcagt taatttcttt ttccaaccag ggcatgtgta
    46381 ttcgagtggt gcaacaggtc tggcacagat tatctctgcc ttgagtaatc actggtttgg
    46441 ttttcatatt ccgatttcgc taagcttcta cgccattaac ttccctttga tggtcttagc
    46501 ttggtatcag attggccata agttcaccgt ctttaccttt atcacggtat ctatgagttc
    46561 cttctttatc cagtttgtcc ctgtggcaac cttgacggag gatcccatta tcaattccct
    46621 ttttgggggt gttgttatgg gtttggggat tggttttgct cttcgaaaca atatctccag
    46681 tggtgggacg gatatcgtca gcctgactat tcgcaagaaa acgggtaaga atgtcggtag
    46741 tatttctttc ttggtaaatg gaactatcat gctgatagca ggtttgacct ttggttggaa
    46801 atacgctctt tattctatga ttaccatctt tgtctctagc cgtgtgacag acgcagtctt
    46861 tactaagcaa aagcgtatgc aggccatgat tgtgacaaat catccagaga aggtaattga
    46921 aaaaatccat aaaaaattgc accgcggagc aaccatgatc cacgatgcag aaggaaccta
    46981 taatcacgag agaaaggcag ttttaatcac tgtcattaca cgtgcagagt ttaatgaatt
    47041 taaacagatt atgacacaag tggatccaag ctcctttgtc tctgtctcgg aaaatgttca
    47101 tattctagga agatttgttg agatagataa ttagtgatta atactcttcg aaaatctctt
    47161 caaaccacgt cagcgtcgcc ttgccgtagg tatgttactg acttcgtcag ttctatctgc
    47221 aacctcaaaa ccatgttttg agctgacttc gtcagttcta tccacaacct caaaacagtg
    47281 ttttgagcaa cctgcggcta gcttcctagt ttgcactttg atttttattg agtataaaaa
    47341 gatcttgggt aacaactcaa tttcagaaga aaaggaagta aatcttccca caataaaacg
    47401 catagtatca agttttttca atacctgata gtatgcgttt ttgcttttaa cgactttttg
    47461 cccagtctct tatttttcga taattttgtg ggcgatcaat tgacggtaac aaatttgttt
    47521 attgttttta ttatcattaa taatctggag aatagtttca agggaaacac gaccaagctc
    47581 taaactattg atatcgacat aggctgccaa gttgagcttg ggattaaccg agtcaaagct
    47641 gagaacaggg acatccagct ggtgtttggc aatatagtta caaacacctt cagctaggag
    47701 gctatcggtt gtgatgatag catcaatttg tggatcgtgc ttgaataatc gcttgctaaa
    47761 tttatagccc ttttcttcca gaaactcgtc ggcaaagtag atgcgattgt tgtcagtggt
    47821 aagtttgtaa tgtttaagcg cctgttcata gcccgttaaa cggtctttgg tcacgaagag
    47881 ctttttactt cctccgataa aggcaatgcg tttgcagcct tttttgatga aatattcagt
    47941 cgcatcaaaa ccagcttgaa cattgtcgtt gtcgacaagt gggatgaaag gagatagaga
    48001 tttacctaag ataaggaagg ggaactgttc ttctgcgacg agttttacga gagggtcttc
    48061 ttcttgggca tagagaaaga ttagcccatc tacacgcttg ccgtagacca tttgtgaaat
    48121 agcgttgaga cgctccttct catctttccc tgttgctatt tgaatggcat agtggttttc
    48181 agatgcgact tgagagatgc cacgtagaac cgatggaaag aaaggattct ggtagaaggc
    48241 gtctgagtca tcaggaagaa ctaatccgat aacctgagta tagctgctta ccaagctacg
    48301 agcgttaagg tttgggtggt agttgagttc cttcatagct ttgcgaacgc gtttttttgt
    48361 ttcgtcgcta atggttgatt tattttgaat aacacgggtt acggttgaag gcgaaacacc
    48421 agcagccttg gccacgtctt taatcgtaac gggcataaaa atctcctatt tatggtaatt
    48481 tggatcacgg aaatgtgttt tataaaagat agtgaccaga tacagaacaa aaagaatatt
    48541 ttgtacagta atcagggttg tcatattttg gccaaataat cccaaaataa gtgtaatcag
    48601 agtcggcaat cctaaacagt tcaagataaa gtgatagcac tctttaaagg ttctaaatga
    48661 aaagaggcgt gatttcttag tgatataaag gagaagacta gctcctagag agacgataaa
    48721 gaaattcaaa ccaaagagga agctcgcacc gagaactagg aagaggctga tatagacacg
    48781 attttgttgg taccagtctt tagaaattgc ttgggtcaag ctgtctttgc ttttgaaact
    48841 ctcagtctga atggctcggt aagagatgcg ggtcagttcc ttgctttcct tgctgatgac
    48901 tagttcattt gtatcgaaat gcagttgcaa gtccttaggt aattccttga tttgacctgg
    48961 accaatcaca atagaagggg cttgactagc tgttccagta taagttaatg tgccatcgac
    49021 aattgtagca tgttcagaga gatcctggac aacctcattt gtcagaggtt catagacatt
    49081 atcgataaaa gtttctagcg gataggtctc ctgggagctg ttttggatag caatgggtac
    49141 catagacaag ctgataagga agatactggt aaagagaagt tgaaaccagt tgagctcgaa
    49201 acgtttggac aggggcttac gaaaccccca aatacttgaa aaataggaaa atggatatgg
    49261 aagcataggt atctttctaa aaggtgtttt ctatatgagt attattatat agagaaatgc
    49321 gctttatttc aagtctaggg ggtaaattgc ttgctacaag gatattattt acagtgacaa
    49381 tcgctaatag tcaatgaaaa tcaaagtgca aaccaggaag ctagctgcag gttgctcaaa
    49441 gcacaacttt gaggttggag ataaagctga cgtggcttga agagattttc gaagagtata
    49501 aaatgaaaaa gggtggcggg gataaattat cccttgtcgc caccacttgt aagtcctgaa
    49561 acaaagttct tttgtaggaa gaagaagaga atacagattg gaagggcgat gaggatagca
    49621 cctgctgaga agtaggcaat cttcaagttt ttcgcattgt taacgaaggt ttggagacct
    49681 acggcaacag taaagtattc tttctcacga agcaagaaac tagagaggat gtagtccccg
    49741 aaaggtccca tgaaggccca gagagcttgt acggcaacca ttgggcgaac aagtggtaga
    49801 acaatttgcc agaagcggcg gaagtgtcct gcaccgtcta gttttgcaga ttcgtctaaa
    49861 gacattggca ctgtatcgaa gtagcctttc atgagccaag cattcatcgg gataccacca
    49921 ccaacgtaga ggaagatgag gaaccagttg tggttaaggg cgttcaacat aagcgccata
    49981 acgaagaagg ctgtcaaagc ggccatagtt ggcaccattt ggatgatcaa gaagaagacc
    50041 aaactttgtt tacgagccaa gaagttgtaa cggctgtaag cataaccagc aagtacgatg
    50101 atacttgttt gaacagccat ggtaattaag gcgataatca aagtgttgag gtaccaagta
    50161 ccgtacaagg tttcagtgaa gaggccttta aagttatcaa aattgaggtc gatattagta
    50221 tctagtttaa aggctgagac gttacctgct ttaaaggctg acatgatggt aatcaacagt
    50281 ggatagataa ttacaattga tagaccaatc aggtaaaggt aagtaaggct ttgagtcagt
    50341 ctacgtttga gtttaattga gttattcatc ttagacgtcc tccatatcaa atgcgtgtag
    50401 tttcttgaat gcgatcatag agattgagat gacaatgata gagataatca aggtaacagc
    50461 tgccgccatt gagtattgag gagatgtacc tgttgtcaaa cggtagatcc atgagatcaa
    50521 gatatcggtt gaaccagctc cacctccgac actaccaggt cctccaccat tgaagaggta
    50581 catgatagag aagttgttaa agttgaaggt gtattggcta atcaaagtag gtgccgcaac
    50641 agccaaaatc attgggaaag tgatgttgcg gaatttttgc caagcgttgg caccgtcaat
    50701 ataagctgct tcgtaaaggt cgttaggaat agattgcaag atacccaaag tcaaaacgta
    50761 gatgtatggg aatccgagcc aaccttgcat cataatcaag gcaatcttag tccaagttgg
    50821 gtctgttttc caaggaataa gagctccatc aaggaaagga aggaatttag ccaagattgg
    50881 caatacttga gtgttgatag caccgacact atcgttaaac atgtttgaga atgtcaagat
    50941 agtgatgaag gctgggacag cccaaggaag aaggaaaata acaccaaaga tacgttttcc
    51001 tttgataaat ggttggttgg caatgatagc tgtgaagata ccaattacga tttgtaaagt
    51061 agaagctgcc aaagcccaaa tgatagtcca agaaagaaca gaaccaaagg cagaacggaa
    51121 ggtactcaag ctccaaatgt ttgtaaagtt ggtcaaacca acccagtcca acaacttgtt
    51181 tggtggcaag tgttggaagt cgtagttggt aaaggcgatc atcaaggtta cgataactgg
    51241 gaagataatc gcgaaggtca tggcaacata agatggaatg atcaagaggt aagggaagcc
    51301 attttcataa atccctttga tcatgtcttt gagtgtgcgt ggaactggaa ttccattgtt
    51361 aatgcgtttt gcaatcgtat gtgcatcttt gatatttgag aaataaaaga gtacataaac
    51421 gattacaaag attagatgga aggcaccacg aatcagcata aagagggaat tatcacgacc
    51481 tggtttgtca ccaagagtga tgaggttgct caattcaggg gttgcaagtg ctaggaagta
    51541 aaggacaaat acgatggtta cactaaggaa gataaaacct ttggcttttt gtttattgta
    51601 aatctgtcct aacccaggaa tgatagacag cagggctgct ttactaggtt gttgcttttc
    51661 catattaact cctttcatag gatactactt ttttataaga aagctaaatt atttgtgtct
    51721 ctattgataa attcaaaggg ggatttgatt tccacccccc ttgaacaaat tttttattca
    51781 ccaaattttt gtttgattgt ttctttgatc aatgttacag catcgttagc agctgtttta
    51841 gcatcttttt gaccacttac agcatcaaag agcatatttt tcgctggatc ccaaactgca
    51901 gacatttgag agatgtttgg cagtggttga gtgttcttga actgtttgat aacagctgtt
    51961 gtcaactcat cgtttttacc ttcagcgtat gaacgagcct cagtattagc tgggatttcg
    52021 ttagtcttat catataatac tttttgttgt tcagttgcaa caaggaagtc tacaaatttt
    52081 tgagaagctt caaggttctt aacggcttga ggaatgaccc aagctttacc accaccgaat
    52141 gcagcatatt cttttccatt tggaagagtt gggatagttg caactccgta gtttacttta
    52201 gcatctttaa aggcttgagc tttccaaggt ccgtcgatga tagcagctgt tttaccttct
    52261 tggaattgag tttggattaa gtttccagca ccttctgtat cttgcatacc tttaggccat
    52321 ttttcgtacc aagatttagc gtagttgata cctgcgatag aaccgtcgtt tgcaagaccg
    52381 atgtctttag cgtctttacc gttttggcca aagacgtaag caccgttacc ggcaagaagt
    52441 ccatatgtat agtagaagtt tgtccagtca gctaggaagg cagtagtttt accatcttca
    52501 ccagcgaatg cgtatttgct atctttagca aggttttcca agtcagcaaa tgtttttgga
    52561 gcatctttca ccaagtcttt gttgtagtac ataacaagtg actcgataac ggcaggagca
    52621 ccgtaaactt taccattagc agctgttaca agagatttag ttgtgtcgtc tgttttagca
    52681 ccatcgctca atttcacttc tgaaagttgt ccgtcagaac caaggctacc tacacggtcg
    52741 tatggagcca tcataacatc agggacatta ccagattggt tgtcaagaga aagtttatca
    52801 agacctccta gagcatcacc agttttaaga gtgactttta ctccagcttc tttttcataa
    52861 gctttagcaa cctcttcaat atagctctta tatccctcgt ctacatatac agtgagttct
    52921 ttgacttcag atgaaccaga atcagcaggc ttatcagcag ttttgcttcc gcaagctacc
    52981 aaaagcaagc tagcaagtgt aacagttcca agcaccgcag tgctcttcat aaatttagat
    53041 gacatagtgt attcctccca aagaatagca agttttattg ataaggaaac gcaaacgttt
    53101 tcctttatga gcttagtata gcacaaatag aaaacggttg caagtatttt ttgtaaaaat
    53161 tttaaaaaat ttttaagctt gatttgatag cataattttg cattttaata gaagaaaagt
    53221 atgaaataga taagaataca gtatttttaa taaaaatata tggaatctgt ctaaagaggg
    53281 aaaccaggag gggctacctc cctggttcta atccctatct attttctatg gacgtttgtg
    53341 ctttgttaca tataaattca agctccctaa ggaggttttg tactttgtag actgtctcgt
    53401 gagagtcaaa ccttaggatt agatagtttt aagttatctt tgctaaaatg agttagtctc
    53461 cttaaaaata atcaaaaaag ttttttaaaa acgcttgcaa ttatgcttga aaaggagtat
    53521 acttataagt aacgcaaacg tttgcgtctg caaaatacgc aacgttccat tattttaaca
    53581 cacgaggtgc tattatgaaa aaacgtcaaa gtggtgtgtt gatgcacatc tcttctcttc
    53641 caggagctta cggaatcgga tcatttggtc aaagtgctta cgacttcgtt gatttcttgg
    53701 tccgtacaaa acaacgttac tggcaaatcc ttccattagg agcaactagt tacggggatt
    53761 ctccttacca atctttctca gccttcgcag gaaacactca ttttatcgat ttagatatct
    53821 tggtggagca aggtttgttg gaagcaagtg accttgaagg agttgacttt ggtagcgatg
    53881 cgtctgaagt tgactatgct aaaatctact atgcacgtcg tcctctttta gaaaaagcgg
    53941 tgaaacgttt ctttgaagtc ggagatgtta aagattttga gaaatttgct caagacaacc
    54001 aatcatggct tgagctcttt gctgagtata tggctatcaa agagcatttt gacaatcttg
    54061 cttggactga atggccagat gcagatgctc gtgctcgtaa agcttcagca cttgaaagct
    54121 atcgtgagca attggcagac aagttggttt accaccgtgt gactcaatac ttcttcttcc
    54181 aacaatggtt gaaattgaaa gcttacgcta acgacaacca catcgaaatc gttggggaca
    54241 tgccaatcta cgtagcggaa gattcaagtg atatgtgggc aaatccacat ctcttcaaaa
    54301 cagatgtcaa tggtaaggct acttgtatcg caggatgccc accagatgag ttttctgtaa
    54361 ctggtcagct ttggggtaat ccaatctatg actgggaagc aatggacaaa gacggctaca
    54421 aatggtggat tgaacgcttg cgtgaaagct tcaaaatcta cgatatcgtt cgtatcgacc
    54481 acttccgtgg cttcgaatct tactgggaaa tccctgctgg ttccgataca gcagcacctg
    54541 gtgagtgggt gaaaggtcca ggctacaagc tttttgcagc cgttaaggaa gaccttggtg
    54601 agctaaacat catcgcagaa gaccttggct tcatgacaga tgaagtgatc gaattgcgtg
    54661 aacgtactgg cttcccagga atgaagattc ttcaatttgc cttcaaccca gaagacgaaa
    54721 gcattgatag cccacacttg gcacctgcta actcagttat gtacacagga acacacgata
    54781 acaatacggt tcttggttgg taccgtaatg agattgatga tgcgactcgt gagtacatgg
    54841 ctcgttacac gaaccgtaaa gaatacgaaa cagtggtaca cgctatgctt cgtacagtat
    54901 tttcatcagt tagctttatg gcaattgcaa ctatgcaaga tttactagaa ttggatgagg
    54961 cagctcgtat gaacttccca tctacccttg gtggaaactg gtcttggcgt atgactgaag
    55021 atcaattgac accagctgtc gaggaaggtt tgcttgactt gacaacaatt tatcgccgaa
    55081 ttaatgaaaa tttggtagat ttaaagaaat aagacaataa tcaggagaca actaaacatg
    55141 ttatcactac aagaatttgt acaaaatcgt tacaataaaa ccattgcaga atgtagcaat
    55201 gaagagcttt accttgctct tcttaactac agcaagcttg caagcagcca aaaaccagtc
    55261 aacactggta agaaaaaagt ttactacatc tcagctgagt tcttgattgg taaactcttg
    55321 tcaaacaact tgattaacct tggtctttac gacgatgtta aaaaagaact tgcagctgca
    55381 ggtaaagact tgatcgaagt tgaagaagtt gaattggaac catctcttgg taatggtggt
    55441 ttgggacgtt tggctgcctg ctttatcgac tcaattgcta ctcttggttt gaatggtgac
    55501 ggtgttggtc ttaactacca ctttggtctt ttccaacaag ttcttaaaaa caaccaacaa
    55561 gaaacaattc caaatgcatg gttgacagag caaaactggt tggttcgctc aagccgtagc
    55621 taccaagtac catttgcaga ctttactttg acatcaactc tttacgatat tgatgttact
    55681 ggttatgaaa cagcgactaa aaaccgcttg cgtttgtttg acttggattc agttgattct
    55741 tctattatta aagatggtat caactttgac aagacagata tcgctcgcaa cttgactctc
    55801 ttcctttacc cagatgatag tgaccgtcaa ggtgaattgc tccgtatctt ccaacaatac
    55861 ttcatggttt caaacggtgc gcaattgatc atcgacgaag caatcgaaaa aggaagcaac
    55921 ttgcatgacc ttgctgacta cgcagttgtc caaatcaacg atactcaccc atcaatggtg
    55981 attcctgaat tgattcgtct tttgactgca cgtggtatcg agcttgacga agcaatctca
    56041 attgttcgta gcatgactgc ctacactaac cacacaatcc ttgctgaggc gcttgaaaaa
    56101 tggcctcttg aattcttgca agaagtggtt cctcacttgg taccaatcat cgaagaattg
    56161 gaccgtcgtg tgaaggcaga gtacaaagat ccagctgttc aaatcatcga tgagagcgga
    56221 cgtgttcaca tggctcacat ggatatccac tacggataca gtgttaacgg ggttgcagca
    56281 cttcatactg aaatcttgaa aaattctgag ttgaaagcct tctacgacct ttacccagaa
    56341 aagttcaaca acaaaacaaa cggtatcact ttccgtcgtt ggcttatgca tgctaaccca
    56401 agattgtctc actacttgga tgagattctt ggagatggtt ggcaccatga agcagatgag
    56461 cttgaaaaac ttttgtctta tgaagacaaa gcagctgtca aagaaaaatt ggaaagcatc
    56521 aaggctcaca acaaacgtaa attggctcgt cacttgaaag aacaccaagg tgtggaaatc
    56581 aatccaaatt ctatctttga tatccaaatc aaacgtcttc acgagtacaa acgccaacaa
    56641 atgaacgctt tgtacgtgat ccacaaatac cttgacatca aagctggtaa catccctgct
    56701 cgtccaatca caatcttctt tggtggtaaa gcagctccag cctacacaat cgctcaagac
    56761 attatccatt taatcctttg catgtcagaa gttattgcta acgatccagc agtagctcca
    56821 cacttgcaag tagttatggt tgaaaactac aacgttactg cagcaagttt ccttatccca
    56881 gcatgtgata tctcagaaca aatctcactt gcttctaaag aagcttcagg tactggtaac
    56941 atgaaattca tgttgaacgg agctttgaca cttggtacta tggacggtgc taacgtggaa
    57001 atcgctgagt tggttggaga agaaaacatc tacatcttcg gtgaagattc agaaactgtt
    57061 atcgaccttt acgcaaaagc agcttacaaa tcaagcgaat tctacgctcg tgaagctatc
    57121 aaaccattgg ttgacttcat cgttagtgat gcagttcttg cagctggaaa caaagagcgc
    57181 ttggaacgtc tttacaatga attgatcaac aaagactggt tcatgactct tcttgacttg
    57241 gaagactaca tcaaagtcaa agagcaaatg cttgctgact acgaagaccg tgacgcatgg
    57301 ttggataaag tcatcgttaa catttctaaa gcaggattct tctcatctga ccgtacaatc
    57361 gctcagtata acgaagacat ctggcacttg aactaatact cttcgaaaat ctcttcaaac
    57421 cacgtcagct ttatctgcaa cctcaaagcg gtgctttgag caacctgcgg ctagcttcct
    57481 agtttgctct ttgattttca ttgagtataa gatacaaatt tatactaata catcttgtaa
    57541 aaaagcgagt ttcgattgaa attcgctttt ttaatgatgt agatttgggt caatcttgtc
    57601 taaaaatagg gaaatcctag atacagtgaa ggctttaaat gctggttttt actgtcctca
    57661 gccttatatt ttttcgtagt tgattacctc atacctcttg tattcgctta cataaagtat
    57721 tataatataa ttgtaggaaa gaaggtgttt ttatgatata cacacttaaa ttggtgttgt
    57781 ttattacctt tcttgtaata agcttgttac ctgataagat ttttggaaaa aataaaaaaa
    57841 tttggaaaat agtttttgca atattgacgg cagtggcagc attgtcattt atgtactaag
    57901 ttattttaag aatgtaggga aataaaccct acattctttt tagttttttc tgttttctaa
    57961 attctattta tccaagcgct tcaacatttc ttgcttcttc gcttcaagtt ctgcacgctt
    58021 ttcttcgatt tcggcatgtt ttttctcgag ttcagaacaa cttgcaccat tgctaaattc
    58081 ttttcgccat caggagatag ggtgagtcga catgtttatt actcacccaa agcagtccta
    58141 caaagcagga attttctgtt acttttttgg aaatagtaac gtttatacag ctttgacact
    58201 tcgtatcaaa gcgccaaaca cactccgagg gatttacaga aagcagaaaa ggaatgatct
    58261 ggtataagat cattcctttt ctctcttttt ctttaagtaa ttatatacaa tgtacgacga
    58321 agtcgtcatt gcaatgctga tccaccacct aaagggaact ttaaacaaca ttgataagat
    58381 aaagaatata aacaacgaaa atacgttata cccaattaat tttattgtat atctcatgat
    58441 taaaagttaa tccttccgtt gttaggaatg gcatcatttt tatcccataa ttgtgctaaa
    58501 taagtccccg atgataataa attcatagcg aattctaaag caacatcatt tacaaaccaa
    58561 ctaactagat atctagaaat tgctgaacga atagcacttt ttgctgcatg ttttcctttt
    58621 actttaatta gatttgcaag gcctgcagta gttcctccta atgctaaagc tattgcagta
    58681 tctaatagag cacccatttg attaactgta ataccttgcc aagctgctct aaatggagag
    58741 tatgtaggtg ggattgtata atcgccttgt aattgtcggt taattacttc tttgatccat
    58801 tgttgtgaga cgtctggatg aaaagattgg atttcgtttg caagtgtatt tatttgttct
    58861 tctgttagag aagtgacagg ttgaagttcc atatttgttt caatttgtga tacttgttca
    58921 gaagcgtata cagctgaaac acttggaatc gctgatacaa ttaacacaat tgacgtcaaa
    58981 aaaaccgaaa taaatttcat taatttgttc atgagctttt ctccttttta tttgtatctg
    59041 cttacatttt atcatatact gttattatag tcaaaaaaat atgctattat gttaaaaaaa
    59101 tatttttcaa aatataaatg gacggattta ttttggattt tatttgttat tttgacctgc
    59161 ctctatattg gtaaccatga tttgtttact ctcaatcatc aagaattctc ttttcgtggt
    59221 agcgtttggg gtctggtact ggccttatat cacttactat tcattgataa gtttgttata
    59281 tcgaatcgaa aataaagatt agagctatgc ttgactgtgt acttttagga ttgattttgg
    59341 aggaagattt tgtctctatt atttattatt ttaaatttat ttattttgta taagatctat
    59401 tctttgaggc gggaaaaatc gaaatacttg atttatacgg cctatatcat atttggggta
    59461 aatgtagtat atggtattca atggttatta agagaactga tttcaactat ttccccttaa
    59521 tgtgtatgga aactataaca aaacgcataa gattaaggtt tgtgtacctg ttcttatacg
    59581 tttttgtcat attgaagatt ttcggtaagt tttctacttt tgcgaagtct agtttatact
    59641 caatgaaaat caaagagtaa actagccgta ggttgctcaa agtacagctt tgaggttgca
    59701 gataaaactg acgaagtcag ctcaaaacat ggttttgagg ttgtagatga aactgacgaa
    59761 gtcagtaacc atacgtacgg caaggcgacg ttgacgtggt ttgaagagta ttagaatgct
    59821 ttcccaacca aaatctctgc ttcgatggta atctctgtca gtttgctcca gtcaattttg
    59881 ctctggtcaa cgtgaaagag gagaggagtc atactgagta gggcttggag ttgttctgct
    59941 gtgatagtct tagtcagaga ggcagtttga ctagatagga tggtaaagtg ttcctggaaa
    60001 tgctccttga tatcttggtt agaatactcc ttgtttgtca gctggtcctg tactctttga
    60061 cggatttctt tgaggtgatt ttcagttggg ataaccttta tcaagatacc gtctttggat
    60121 aaaacgcgac gaaattctcc atagttggca ggtgagaaga tatcaagcag aatatccatg
    60181 ttagcgtctt ttataggaag tcgtgccaag tcgccaacga accaattgac tgcccagttg
    60241 ggttcacttt tagcagcgat ttggactgaa tctttggaga tgtcaaaggc atagaaagtt
    60301 ttttcagagt gactttcttg tagtttgcga gaatagaatc cttcaccaca accgatatcc
    60361 aaaattgttg tggtagtttt tgagcttgca agcaagtcag atacagcatc taagatagct
    60421 tggtaaaagc cggcttctag gatttgttga cggttttgaa aattttcctt gtcatagtta
    60481 gcagattgct tgatttgagg tgctagattg acatagccaa atttcgccaa gtcaaaagaa
    60541 tgacggttgc agcacttgaa attagtctct aacagagtca gattttcttg acagatagga
    60601 caggcaaagg cagtcgcaga agcaaaacgc tgaagtttgg gcttgagatt tgtattcata
    60661 gtttaagtgt atcaaaagag gcaaatgaaa gcaactttag gaataagtag atgggagatt
    60721 cagtagaaat aaaactaatt ctccaattta tagaatgaaa ttgcttttat atctaaattg
    60781 ctatataata atgataagga ggaaataagt atgttaaaag aagtattaac cgttgcaaaa
    60841 gttgcgaaaa aatcttcatt atttttgggt ggtgtcgcat ttggtaccct tggtttgaaa
    60901 atcttagcaa gtaaggaagc taaaaaaggt tattctaaag ctttggctaa ggcttacaag
    60961 ttgaaagacg agctagatgc atctgtttct gtcgtgaagc aacatggaga cgatgtcttg
    61021 caagatgcca aatatttgta cgagcaagag aaaaaagaag agcaattaga tagccttata
    61081 ggtgaataat atgtctttta aagtgctaca tagaggatac caacatatcc gactatcatc
    61141 ttctttttca cttaccttgg atattcaaga ctatcttcgt tccttggcga gagatgaaaa
    61201 ggggattgag tctatccagt tttacatgga tcaacagcac tttactctac gcataaaaga
    61261 aggcttttct gtattagata atgcagaagc ctttttaaaa agaattgata aagggaaagt
    61321 ttctgagttg atgactcttc ccattcgtag agaagagagt gcttattcta ttgtttcagg
    61381 tgcagcggtt aagcgtgtac tttttcgtag ttttgtgccg tatcctattc gctatatatg
    61441 gacttgttat caggctttgg gttatattag agaagcctat caaacactag cgcgtaagga
    61501 actaacgatg gaagtcttgg actgttcggc tattttattg tctttgttta tgaaccaatc
    61561 caagacagct agcaatatca tgtttatgct tgatttgggg aatcatttag atcagtggtc
    61621 cttgaaaaaa actgcaacag atttagaaca gagtcttctt gcaaaagaga gcgatgtatt
    61681 cctagtacag ggcgatacag ttgttagtat caagagttcc gatgttcaaa taggagatgt
    61741 cttgatctta tctcaaggaa atgaaattct gtttgatgga caagtagttt caggtttagg
    61801 tatggtcaac gaaagttcct tgacaggaga gagttttcca gttgaaaaaa gagagtctga
    61861 tttggtttgt gcaaatacag tattagaaac tggagagtta cgcatttgtg taacagataa
    61921 tcagatgaac agccgtattt tacagctgat tgagttgatg aagaaatctg aagaaaacaa
    61981 gaaaacgaaa caacgctatt tcatcaagat ggcggataaa gtcgtcaaat ataatttctt
    62041 gggggctggg ctgacttacc tattgacagg ttctttttct aaggctattt ctttcctatt
    62101 ggtcgatttc tcctgcgctt tgaaaatctc tactccggta gcttatttga cagttatcaa
    62161 ggaagggttg aaccgtgaaa tggtgattaa ggatggagat gttctggaga aatatctgga
    62221 agttgatact ttcttgtttg ataagacagg aacaatcaca actagttatc ctatagttga
    62281 aaaggtgtta ccttttggag actatagtga ggaagatatt ctcagaatca gtgcctgtct
    62341 tgaggaacac atttatcatc ctattgctaa tgccatcgtc aagcaagctg agatagaggg
    62401 aattgaacat gaggaaatgc atgggaaact ccaatatatc gcaagcaagg ggatcaaatc
    62461 tcatatagat ggccaaccag ttcttattgg gaattatgtc ttgatgcagg atgagcagat
    62521 tcatatcagt tcagaacaaa atgctttaat tgaagagtac aagagtcact acaatctctt
    62581 attcttggct tatcaaaatg aattgattgg aatgttctgc attcatactc ctttgagaaa
    62641 agaagcaaaa acagccttgg ataaacttaa ggcacagggg aaaaaattga ttctggcaac
    62701 aggagacacc ttgattagga cagaggaatt agtcaaagat ttgccctttg atcaggtcta
    62761 tacagacttg aaacctgatg ggaaatttga gttagtagag aaactgcaga aagcaggtca
    62821 cactattttg atggttggag atggattgaa tgactcagcg gctttaactc tatcagatat
    62881 cggtgtggtg atgaatgaga gtgcagatat ttctaagcag atgagtgata tcttattgtt
    62941 agataatcgt ttggatttct tccaagagtt ggattcgtta tcatcatctt tgcaaacact
    63001 catcaagaaa aatattcagg ataccgttgt cgtaaatagt agtttgattg gctttggctt
    63061 atttaattgg ttcagtcctt caaatctctc tatcttacat aatctaacaa ccttgcgcat
    63121 tgtcctgcgt agcctgtcta ttaaaaatag atagatgaga gttattaaca gcaaaaagga
    63181 gttacctttc tcgaggttaa ctcctttgtt tatagataaa tgttgaatag tttagtaagt
    63241 caatacaaag tattttttct tcccacgacg gataacagtc agttcatttt ctaacttatc
    63301 agcgtcactc aagacatagt caaggtcttg gatgcggtcg ccgtttacgt agatggctcc
    63361 gttttggacg tcttcacggg cttggcgttt tgagttaact ataccagatg agacgagcag
    63421 ttccacgata ttgttgtttt cgtctgcctg tacttggtag tttggcacac cacgaagtcc
    63481 ttgtttgagc tctttgacag aaaggttttt gatgtttcct gcaaagagtt gctcagtgat
    63541 gttaagtgct tctttgtagg cttcttctcc gtgaacaagt gtaacaactt cacgagccaa
    63601 gactttttga gccaagcgtt cgtgtggcgc tgcttcaaat tgtttacgaa tatcttcaat
    63661 ctcatcaagt gacaagaaag taaagatttt caagaagcga acagcgtcag cgtccatcac
    63721 gttcatccag aattggtaca tttcgtatgg agaagtcttt tcgggattga gccagacggc
    63781 atttccttct gatttaccaa attttttacc agttgcatct gtgattagtg gaacagtgat
    63841 aacgtgacca gtcttgtccg ccttacgacg aagcaattcg gtaccagctg tcatatttcc
    63901 ccactggtca gaaccaccga tttgaagagt gacattatgg tcttggttaa ggacgaagaa
    63961 gtcgtaccct tgcatgattt ggtaagcgaa ctcagtgtaa gaaattcctg tttcgatccg
    64021 ttttttaaca gattccttac tcatcatgta gttgaccgtg aagtattttc caatatcacg
    64081 gaggaagtca atgaagctga tgctgccaaa ccagtcgtag ttgttgacca tgacagcctt
    64141 gttttcgcca ttttcaaagt caagaaaacg agaaagttgt ccttggatag acttgaccca
    64201 gccatctact gtgtcttttg tttggagact acgttcagca tctttgaagg acggatctcc
    64261 gatgagacct gtagcaccgc caacgagcgc ataaggtttg tgacctgcta gttgcaagcg
    64321 acgacttgtc aagattgcga caaggtggcc taggtgaagg ctgtcagcag ttggatcgta
    64381 gccagtataa taagaaactt gaccttcttc tagggcttta cgcaaagctt cttcatcagt
    64441 cgtttgaaat atcaaaccac gctcttttag ctcatcaaaa atgtgcatgt gtcttttctc
    64501 ctttttaaaa ttattgtttc tacctattgt atcacaaact tgggcaatag cctagcagaa
    64561 tagggaagaa agtggctatt ttattgaaaa tttccctttt gtggtataat agataaagtg
    64621 aggaaatcca tgcaaaatca attaaatgaa ttaaaacgaa aaatgctgga atttttccag
    64681 caaaaacaaa aaaataaaaa atcagctaga cctggcaaga aaggttcaag taccaaaaaa
    64741 tctaaaacct tagataagtc agccattttc ccagctattt tactgagtat aaaagcctta
    64801 tttaacttac tctttgtact cggttttcta ggaggaatgt tgggagttgg gattgctttg
    64861 gggtacggag tggccttatt tgacaaggtt cgggtgcctc agacagaaga attggtgaat
    64921 caggtcaagg acatctcttc tatttcagag attacctatt cggacgggac ggtgattgct
    64981 tccatagaga gtgatttgtt gcgcacttct atctcatctg agcaaatttc ggaaaatctg
    65041 aagaaggcta tcattgcgac agaagatgaa cactttaaag aacataaggg tgtagtaccc
    65101 aaggcggtga ttcgtgcgac cttggggaaa tttgtaggtt tgggttcctc tagtgggggt
    65161 tcaaccttga cccagcaact aattaaacag caggtggttg gggatgcgcc gaccttggct
    65221 cgtaaggcgg cagagattgt ggatgctctt gccttggaac gcgccatgaa taaagatgag
    65281 attttaacga cctatctcaa tgtggctccc tttggccgaa ataataaggg acagaatatt
    65341 gcaggggctc ggcaagcagc tgagggaatt ttcggtgtag atgccagtca gttgactgtt
    65401 cctcaagcag catttttagc aggacttcca cagagtccca ttacttactc tccttatgaa
    65461 aatactgggg agttgaagag tgatgaagac ctagaaattg gcttaagacg ggctaaggca
    65521 gttctttaca gtatgtatcg tacaggtgca ttaagcaaag acgagtattc tcagtacaag
    65581 gattatgacc ttaaacagga ctttttacca tcgggcacgg ttacaggaat ttcacgagac
    65641 tatttatact ttacaacttt ggcagaagct caagaacgta tgtatgacta tctagctcag
    65701 agagacaatg tctccgctaa ggagttgaaa aatgaggcaa ctcagaagtt ttatcgagat
    65761 ttggcagcca aggaaattga aaatggtggt tataagatta ctactaccat agatcagaaa
    65821 attcattctg ccatgcaaag tgcggttgct gattatggct atcttttaga cgatggaaca
    65881 ggtcgtgtag aagtagggaa tgtcttgatg gacaaccaaa caggtgctat tctaggcttt
    65941 gtaggtggtc gtaattatca agaaaatcaa aataatcatg cttttgatac caaacgttcg
    66001 ccagcttcta ctaccaagcc cttgctggcc tacggtattg ctattgacca gggcttgatg
    66061 ggaagtgaaa cgattctatc taactatcca acaaactttg ctaatggcaa tccgattatg
    66121 tatgctaata gcaagggaac aggaatgatg accttgggag aagctctgaa ctattcatgg
    66181 aatatccctg cttactggac ctatcgtatg ctccgtgaaa atggtgttga tgtcaagggt
    66241 tatatggaaa agatgggtta cgagattcct gagtacggta ttgagagctt gccaatgggt
    66301 ggtggtattg aagtcacagt tgcccagcat accaatggct atcagacctt agctaataat
    66361 ggagtttatc atcagaagca tgtgatttca aagattgaag cagcagatgg tagagtggtg
    66421 tatgagtatc aggataaacc ggttcaagtc tattcaaaag ctactgcgac gattatgcag
    66481 ggattgctac gagaagttct atcttctcgt gtgacaacaa ccttcaagtc taacctgact
    66541 tctttaaatc ctactctggc taatgcagat tggattggga agactggtac aaccaaccaa
    66601 gacgaaaata tgtggctcat gctttcgaca cctagattaa ccctaggtgg ttggattggg
    66661 catgatgata atcattcatt gtcacgtaga gcaggttatt ctaataactc taattacatg
    66721 gctcatctgg taaatgcgat tcagcaagct tccccaagca tttgggggaa cgagcgcttt
    66781 gctttagatc ctagtgtagt gaaatcggaa gtcttgaaat caacaggtca aaaaccaggg
    66841 aaggtttctg ttgaaggaaa agaagtagag gtcacaggtt cgactgttac cagctattgg
    66901 gctaataagg caggagcgcc agcgacaagt tatcgctttg ctattggtgg aagtgatgcg
    66961 gattatcaga atgcttggtc tagtattgtg gggagtctac caactccatc cagctccagc
    67021 agttcaagta gtagttctag cgatagcagt aactcaagta ctacacgacc ttcttcttca
    67081 agggcgagac gataattctc taaatgaagt ggccaatcaa tggattgcca cttttttctt
    67141 ttcgtgttac aataagggta tgaatcagta tcagaaaaag attgttaacg gaaaaattta
    67201 ttcgctccta tccggcttaa tatggggaat ctgtggaatt ttaggagagt acttctttac
    67261 tcattatcag gtgtcttcgg gctggattac ctctatgcgt ttgacactgg cagggagtct
    67321 tgtactcatt tggtctgcaa tacaattaaa atcgcaagtg ctagatattt ggcgagacaa
    67381 gaaaaattac ctgccctttt tagcctatgc tattttgggg attttttcag ttcagtattt
    67441 tttctatctc tgtgtagaat actcaaatgc tacgacagca actattttac agtttattag
    67501 ccctgtcttt atcctctttt acaatcgctt ggtttatcaa aaacgagcgt caaaaagcgc
    67561 tgttttctat gttttggttg ccatgctggg tgtttgcttg atggcgacaa agggagatct
    67621 ctctcagtta tccatgacgc cgctagctct tataacaggt ttgctgagtg ccatgggtgt
    67681 tatgtttaat gttatcttgc cccaaccttt tgctaagcgt tatggttttg ttcctacggt
    67741 tgggtggggg atgattttgg caggtttgtt tagcaatgtc ctctcgccgg tttatcagct
    67801 ttcctttact cttgatattt ggagtatctt gatttgcctc attatcgctt tctttggaac
    67861 ggcttttgct tttttcattt ccatgaaggc tgtgtccttg gtttctcctt tggtggttgc
    67921 cgttatcagt gccagtgaac ctctctcttc tgctctcttg agtgttttgt tcttaggatt
    67981 agtagtggat tggtccctcc ttctagctat agccttgatt attttaccca tgatattttt
    68041 gtctatagaa gaagcgaaag aaagtagata aaaagtcttt tcttaattct aaaagtgtgc
    68101 tatactagaa tagtcaataa aacacgggga gatagttgtg aagagtgttt ttaggttgta
    68161 tcggtagggg cttgctttta ccgacagcac gttttatctc acatccctaa aaaatcatac
    68221 ctaaaaacag acaaaaaggt ttcaaatttg aggccttttt tggtagaata ggtatcatta
    68281 taacgaacca ggaggcacct atgactgcta caaaaatgaa cgctcaagaa attatccaat
    68341 ttatcgccaa tgctgaaaag aaaaccagtg tcaaagtaac ctttgagggg caactcgcaa
    68401 ctgctgtgcc tagctctgtt gtcaaactag gaaatgtcct attcggagac tggaaggacg
    68461 tggctccgct tcttgaaggt ttggtagaaa atcaagatta tgttgtcgag caagatgctc
    68521 gtaattctgc agttcctttg ctagataagc gtgctatcaa cgctcgtatc gagccaggtg
    68581 ctattatccg tgaccaggtg gaaattggtg acaatgctgt tatcatgatg ggagctgtta
    68641 tcaatatcgg tgctgaaatc ggtgctggaa ccatgattga catgggtgcc atccttggtg
    68701 gccgtgccat cgttggaaaa aatagccacg ttggtgcagg tgcagttttg gcaggtgtga
    68761 ttgagccagc tagtgctgaa ccagtctgtg tcggagacaa tgttctcatc ggggctaatg
    68821 cagtggttat cgaaggagtc caaatcggta gtggttcagt tgtcgcagca ggagctattg
    68881 ttacccaaga tgtcccagaa aacgtggtag tagcaggtgt tccagctcgt attatcaaag
    68941 aaattgatgc ccaaactcaa caaaaaacag cgctagagga tgcgcttcgt accttgtaat
    69001 tgtaaaagta aaaaagaggc ggaacccttt ttccagcctc tttctgctat atcggaggac
    69061 agatagatgt tagatttgat tcagactaga cgagatttac accagattcc agagattggc
    69121 ttggaggagt tcaagactca ggcttatttg ctggatgtga ttgagaaatt gactacgggc
    69181 aaggattttg ttcaaattcg tacttggcgg acagggattt tggtctacct gcagggaagt
    69241 cagccggagc gaaccattgg ttggcggaca gatattgatg gcctgcctat cgtcgaacaa
    69301 acaggtctac cttttgcttc tcaacaccaa ggtcgcatgc atgcctgtgg ccatgatttt
    69361 catatgacca ttgccttagg ctgtcttgag cgcgcccttg aagagcaacc taaaaataat
    69421 ctgctcttcc tatttcagcc tgctgaagaa aatgaagctg gtggtatgct catgtatgag
    69481 gacggtgctt ttggagattg gttgccagat caattctatg gtctccatgt tcgtccagat
    69541 ttgaaagtcg gccagattgc gactaatact catacactct ttgcaggaac ttgcgaggtg
    69601 aagatccgtt tcaaaggcaa aggtggccac gcagcttttc ctcatgaagc caatgacgcc
    69661 ttggtggcgg ctagttactt tgtgacccaa gtgcagtcag ttgttagccg caatgtcaac
    69721 ccaatcgagg gagcagtggt gaccttcggc gtttttcaag ctggaaccac caacaatgtc
    69781 atcacagaca cagctttttt acatggaact attcgtgcct tgacacagga catgagtctc
    69841 ttggtgcaaa agagggtaaa aacagttgca gaagcggttg cagctgcctt tgatatggaa
    69901 gtcgaagtgg aactcaaaca aggtggatac ctacctgttg agaacaatcc agccttggcg
    69961 cgtgaactga tggacttctt tgacgaaaaa gacggaatcg agttgattga tatcgaacct
    70021 gctatgacag gcgaggactt tggttatctc ctttcaaagg ttgatggtgt gatgttttgg
    70081 ctaggtatcg atagtcccta cgctcttcat caccctcaaa tgagtcctaa ggaagaagtc
    70141 ttagctattg gggtggctgc ggtgtctagt ttcctgaaaa agaaggcagc agaatagagg
    70201 gcttgtctat gaaatcggaa ttacgcaagc aagtcttgca tgaaatgaag gctttatctc
    70261 aagaacaaaa acaggctata gaccaagctt taaccgagcg aattttacaa cacccctttt
    70321 atcaagaagc caaggtcatc gcaacctacc tctctttttc tcatgagttt caaacgcggg
    70381 aactgattga gcaggcgctg aaggacggca agagggtttt gacacctaag acctatccca
    70441 aggggcgcat ggactttgtg gtctatgatc cgcaacagtt ggtaaaaact tcctttggat
    70501 tactggagcc acagggagat ttggaagttg tggatgtttc aaagattgat ttgattcatg
    70561 ttcctggtct ggcttttacg acgaaaggat atcggatagg atacggtgga ggctattatg
    70621 accgctatct ggaacatttt tctggtcgtg ctttgagtac gatatatcct tgtcaaattc
    70681 aggactttat acctgaaaac catgatattc ctgttcagga ggtattaatt gatgaaggaa
    70741 atctttgata gacgttaccc tgtgacgagt ttcttcctct tagtgacggc cttggtattt
    70801 ttactaatgt tggtcactgc aggcggaaac tttgacaggg cagatacatt atttcgattt
    70861 ggagccatgt atggtccagc tattcgcctc tttcccgagc aggtttggcg tctcttgtct
    70921 gccatttttg ttcatattgg gtgggaacat ttcattgtta atatgctttc actttattat
    70981 cttggaaggc aggtagagga gattttcggt tctaagcagt ttttctttct ctatctttta
    71041 tcaggaatga tgggcaatct ctttgttttt gtatttagtc ctaaatcctt agcagcaggc
    71101 gcctctacct ctctttatgg gctatttgcc gcgattattg ttcttcgcta tgcaactcgc
    71161 aatccttata tccaacagct agggcaatct tatctgacac tttttgtggt taacattatt
    71221 ggaagtgttc tgattccggg aatcagccta gcaggccata tcggtggagc agttggtggc
    71281 gcatttctag cagttatctt tccagttaga ggagaaaaac ggatgtataa caccagccag
    71341 agattaggag cggtagtctt gttcgtagga ctcgccattt tgcttttcta caagggaatg
    71401 ggaatgtgac gaagttgaaa gaaaatttgt gtaatgaaaa aagataaggc attttttgaa
    71461 ccttatcttt ttattttatt ccttctcagt caattctttt ccaagttgga tgaggtaatt
    71521 cttcaaatca tctttgactt gtggatgttt gagggcgtag tcaatagatg ttttcataaa
    71581 gccaaactta tccccaacgt cgtaacgagc tcctgtaaat tcacgggcaa aaacacgctg
    71641 tgttttatta agagtatcga ttgcatcagt aagttggatt tcatttccag caccaggagc
    71701 ttgcttttcg agaatctcaa aaatttctgg tgtgagtaga tagcgaccga tgattgccaa
    71761 atcacttgga gtctcctcgg gagctggttt ttcaacgaaa gtttcaacgc tatagagtcc
    71821 attgcttcct tcgccttgag gagcaatgac tccatatgat gaaacgtctt cgtgtggtac
    71881 gggcatgaca gcaatggttg atgcgtgtgt cttttcgtaa tcattcatca gttgttttgt
    71941 caaaggaacg gccttctcgt ctgtgatatc catcaagtca tcaccaagca taacgacaaa
    72001 aggttcattt ccgacgaaag ccttggcttg caaaacagca tctccgagac cgcgtggatg
    72061 agtttggcgg atgaaatgaa ggcgcatgcc agttgtttca tctaccaatt ttaacaaatc
    72121 cgttttgcct ttttccttaa gattgtattc aagctcaaag tttgaatcaa agtggtcctc
    72181 aatagaacgt tttgatttac cagtgacaac tagaatatct tcaatacctg atttgagagc
    72241 ttcttccacg ataaactgga tagttggttt gtctacgatt ggcaacattt ctttggcaag
    72301 ggccttggtt gctggtaaaa atcgagttcc tagtccagca gcagggatga ctgcctttct
    72361 aacttttgat gtcataaaaa tcctttctat agagggttaa gaccactcat tttctgcttt
    72421 aaattcattg ttcatgatgt cataaatggc atctttgata ttggttccgt gataaataac
    72481 ttggtaaata gcctgtgtaa tgggcatata gactccaagt tcttgggcta gttcataggc
    72541 tgatcgagtc gttgaaattc cttcgattac catgcccata ttagcttcta tatcagctag
    72601 ggattctcct cgtccgagag catctccagc tctccagtta cgagagtgga tggaagttcc
    72661 cgttacgatc aaatctccca caccagataa gccgctatag gtcaatggac tggccccgag
    72721 tgctacccct aggcgggtga tttctgctaa acctcgagcg atgatggctg ccttagcatt
    72781 atcaccaaat ccaagaccat gtaaagctcc agcaccgaca gcaataatat ttttaagagc
    72841 accagcagtt tcaaccccga taacatccgt attggtataa agtcggaagt agtgattact
    72901 aaatagctcc tgaacgtatt gagctgtttg taaatcttta gaagcagcag ttattaaagt
    72961 taggtcacgc acaatggtct cttctgcatg actaggccct gaaacaacga cgatatcact
    73021 acggagatgt tcaggaattt cttcttcaag aatggttgat aatcgtttat ggctatcagg
    73081 ttctaatccc tttgatgcgt gcatgatgat aaccttatgg tccaaggttt gtgcaacttg
    73141 ctgggcaaca agtcgtgtca cttttgttgg gacaacaaac aaaatcgcat ccacattttt
    73201 caatgtttct gctaagtcgg tgtaggcaat gatattttcg tctagaacga catctttaaa
    73261 gtagtgctta ttagtatggt gtgtattaat ttcattgatt tgctcgggaa gatttcccca
    73321 aatacgtacc tcgtgtccat tgtcatttaa gacttgtgaa agggcggttc cccaagaacc
    73381 aggccccaag acggcgacgg tttgtttttc catgatttcc tccttgatag atttctttct
    73441 cttattatat catattctag gggattttgc acttgcatta ctggggtatg gtggaaaaat
    73501 acacaaaaac cgagatgaag ggataaactc ttagtcttta gttaaagtga gaagaaatct
    73561 gatagatagt ttgattcagt atatgctata aatagaggta aacaactcaa gaggttcttg
    73621 taggttgcga gagtttggcg aaaaaattaa aagattacgt ttggctaaaa aaatcagtcg
    73681 ttcagaattt tgtggtgatg agtctgaatt aagtatccgt caattaatta gaattgaaaa
    73741 tggagaatcc agaccaacac taacaaagtt aaaatatatc gctgaacgtt tgggggttga
    73801 agattacaag ttgatgccaa gttatataga gttggataag gaatacctag aattgaagta
    73861 tttcttgatg aggactccta catacgaaga tgaaactatc gcccaaaaga aagagagtgt
    73921 ttttgctaag atttttgaag agtattatga taggctacct gaggaagaaa gatttatcat
    73981 cccaaattat tcatatctgg cactaacgaa ctacacagtt caaaaattac cagaaaagct
    74041 agttgaaata ctgtccttct ggtgattttg cttaatttct gatgttcgag acatgattga
    74101 ggagatttgt cttgatttgt tcctaaaaag ggcagactcc tcccttggtt tgatgatcga
    74161 tttttaaaat aaggtgactt attctgttag gcatgttttc ccctgccctc tagctctata
    74221 tggaacaggg agtatcagat ttatctggca gattgtatca aataaggctt gaaactgttt
    74281 tgaataggcg tatagacttg agaatttgag aatatgtcgt ctagcagtag agacatattt
    74341 tccggcaata tgaaggaaga gacgtcgaaa acgcttgata gtcaaggcct ttacttcatc
    74401 accagctagc tgttttaaaa agaggtagag attataagcc agacagccca tcatcatacg
    74461 tatttcattc ttaatcatgg tcgaactatc tgtcttatcc ccaaagaatc ctgctttcct
    74521 ttctttgata aagttttcag cattccctcg ttcacgatag aattgaaaca gctcctcact
    74581 atctacactg cttaaattgg tcaaatgaac ttttttacaa ccataaaaaa gagcacttaa
    74641 tcgtactctt ctcttttaag gattagttga attaattcaa atccactagt tctcctgttt
    74701 ctaggtagac taggcgttca caaatgttag caatgtaatc agcgaagcgt tccagatgcc
    74761 ctatgatata aaggtattgt gttccattgg gaattgaggt ttcttggtct ttcataagtc
    74821 caatgatttc ctttgataag gcataataat attggtcaat ctgttcatct ttttgagcaa
    74881 tactaatagc ttttgaggct tggtgcaaag gaaaggcaac caataaatcg gctagcatgc
    74941 tgagggataa tttacccatt tggtgtaact gttcttcgcc aggggctagt tgattttctt
    75001 ttagttgcaa aacagctttg gcaatgcctg ccatatggtc tcccatacgt tcaaggtctg
    75061 aacaagaaga catgatgcta atcacaaatc gaaggtcaga cacttgtggc tgctgcaagg
    75121 ctaacaaacg ggcacaggtc aattcgatag cgctttgacc ttggttgata gcatgatcct
    75181 tattgataat tagctttgcc atctccttgt ctttggaggc taaggccagt aaggcttttg
    75241 aagctgtttc aaggacaagt tgccctagtc ctaaaaagga ttgttctaat tcatgcaatt
    75301 ctaagtcaaa ttgatttctc ataggttttt ccttccttat ccgaaccgtc ctgaaatata
    75361 gtcttctgtg cgctgatctt ttggattggt aaacacgtca acggtatctc caaattcgca
    75421 aatttctcct gttaagaaaa aagcagtttt atctgaaata cgtgaagctt gttgcatgtt
    75481 atgggtaaca atgataatcg tataatcctt ttttagttgc tgaatgaggt cttcaatttt
    75541 taaagtggag atagggtcta aggctgaagt cggctcatcc attaacagaa tatcaggttc
    75601 tactgctaaa gctcgcgcaa tgcaaaggcg ttgctgctga ccgccagata aggacatggc
    75661 actcttttta agatcatctt tgacttcttc ccaaatggct gcccctttta aagatttctc
    75721 cactaaggca tctaattgtt ttttgtctcg aataccatgt gtccttggac cataagccac
    75781 gttatcatag atagacatgg caaagggatt aggctgttga aaaaccatcc ctacacgctt
    75841 gcgtagctga ttaaggttga atttgctact ataaatatct tgctcatcta agaggacttg
    75901 gccttcaata tggcaagaag gaaccaaatc gttcatccga ttaagggttt ttagaaaagt
    75961 tgatttgcca caaccagatg ggcctatcaa ggcagtaatc tgtctttctg gtaattgaat
    76021 cgaaatattt tttaaggctt gaaaatcccc gtaaaataag tctaggtgtc tgactgaaaa
    76081 tgttcccata ctaggaagct cctttcacaa gtttccgaga taataagctt gatagagtat
    76141 ttatcattaa aacagtaata atcaaaatca cgccggtagc ataggcttca ttgacatgta
    76201 gcccctcact tgacagcata tacatatgta gggctagaga acggcctgaa gacatgagac
    76261 tacttggcgt attggtagag gtacctaatg tatacatgag ggcagctgtt tcaccaacga
    76321 tacggccaat agctagtatc actccagcta aaatacctgg catggcaact ggtagaacaa
    76381 ttctaaaaac agtccgtaac ttacctgccc caagtccata acttgcttga cgcatgctat
    76441 cactaacaga taaaagggct tcttctgttg agcgaataat gactggcaac accatgataa
    76501 ctgaggttaa gattcctgat aacagagagt attgaaaacc taagaagact acaaagaaga
    76561 gcatgccaaa cagaccaaaa acaatggaag gaatcccaga caaggtatct gaggccaatc
    76621 gcatgatttt aacacaaaga gaatcttttt ttgtatattc cacaagataa aaaccagcaa
    76681 aaatccctat gggcaaggct aaaagaagag caccaaaaac cagaataacg gtggaaataa
    76741 tcgctggcat aagggaaatg ttctcagaag tataagtcca agaaaagagg gatagactta
    76801 gatgaggtaa gcctttgatg aggataaaac caatgattaa aaagagagag ccaaaggtta
    76861 aagctgaaaa acaataaacg agaagtttta gcaggtattt actcataaga tgattttcct
    76921 ttcaagtagg caaagtaggc attaatcaag agaataagga aaaagagaac tgctgaggtt
    76981 gcaataaggg cttccctatg ctgacctgat gcgtaagcca tttccagaac aatattggtt
    77041 gttaaggttc tggttcctga aaagagtcca cttggaataa tcggctggtt gcctgccacc
    77101 aaaataactg ccatggtttc acctactgcg cgaccgattc ctaaaataac tgctgataaa
    77161 ataccagatc tcgcagctgg caagatgaca ctaaaaatac tccgttcatg actagctcct
    77221 agagccaagc taccagaata atacgttttg ggaactgttc ggatagcaga ttctgacaaa
    77281 ctgataatgg ttggcaaaat cattattcct aatagtaacg aagcggttag gacactcatg
    77341 ccatttccta aaaaggttct aatccaaggc accaataatt gtaggccgaa aaaaccataa
    77401 acaatagatg gaatggctgc catcaagttg atagctgatt ttaagaagct atagacgggc
    77461 tttggacaat aataaaccat aaacaccgat gtcaagatgc ctgttggcac cccaatcaca
    77521 atcgctccta aggtaattaa taaggaacca acgatcattg gtaaaatacc atagcttgcc
    77581 ggaatgttcg ttggcgacca atcactgcct aataaaaaac gggcaaagcc gtagttagct
    77641 atgaaaggta agccattact aaaaataaag aaacagatta gcaaaatagc tacaacagct
    77701 actgttgcac tcatgaaaaa aattgcccta aaaactgctt ctttgaaggc ttgttttgtc
    77761 acatcttgtc ctttctagtg aagaaagtaa gggagatacg acacctccct acttgccttc
    77821 tttatcttat tgtacgatga aacgtctgca tctctttaga gatttatgga gcaaacattt
    77881 tatttaatct tgtcccaggt ggttaatttg ccactaaaaa cgtccgcaag ttcagccata
    77941 ctgacttggc ttgccttatt gtcattattg accacaacag caataccgtc taaagcaata
    78001 gcatcatggg tgagactctt accttcttca ggagttaatt ccctagaaac cataccaata
    78061 tcagcggttt tctccttaac agcggtaata cctgctgaag acccattaga ggtaatatca
    78121 atcgtaactt ctggattttc ttttttataa gcttctgcta atttttccat taaagaagat
    78181 actgaagtgg aacctacaac agacaacttg cctgataagt gttggcttgt atattctgtg
    78241 gtttcggttt tagcttcaat aaatttatta tctgtgacca cttgttgacc ttgtttggag
    78301 tggataaagc tgataaaatc ttgacctagc ttggaaagat tagaagacca aacaatgttg
    78361 aagggacgtt gaagagggta ttcaccatct aaaactgtgt ctcgactagc cttgacacca
    78421 tcaatctcta aagccttgac agatttcgtt aaagatccca aggagatgta gccgatagca
    78481 ttagcattcc cttgaactgc tgagagaaca ccttctgtac tattttgaat cacagctgtt
    78541 ttggcagtgt tgtcaatttt tttatcaccg tcttttttga gaatccctgt gatttctgtg
    78601 aaggcacccc gtgttccaga gccattttct cgtgaaatca cctcaatcgt tcctgaagct
    78661 gactgtttgg aagcagctga ctgattgcca caggcaacaa gcccaaatcc tgataagcca
    78721 atggctgcaa gagtaagcat ttttttgaat ttcataataa tcacctttat ctctatgtat
    78781 ttttcttgtg taggcttact acatttatag tctaacaagt ctttgtaaag gtttatccct
    78841 gattcatgta aagattgtgt aaagaatcaa aaaaagccac ttttgaaaaa tggctgctcc
    78901 taaaaatagc tttaaaaatt attagtcctg tgcgaaagat tggttaggaa gaaaaatcgt
    78961 gaagcaactg cctctgccaa gctgactcgt caccgtgact tggccaccta ataattgact
    79021 gagttctttg acaatggcaa ggccaagacc agtgccacca gtttgtctgc ttcgaccttt
    79081 attaactcgg taaaaacgtt caaaaatacg atcctgctct aattgactaa taccaatccc
    79141 tgtatctgat acagaaatct taatgccttc gttcaccttt tgggtcttga cctcaatttt
    79201 tcccccttgt tcagtgtaac ggatggcatt ggataaaaga ttgagtaaga tttgggaaag
    79261 taattgacta tctgatacga gggtgacatc atctggcacc tgcaccttta gctgtaaatc
    79321 cttcttcttg agctgaggtt gcaagctttg agtcaaatcc tgtacaaatt ctgccaaaga
    79381 aagggtcgtc cattgtatag gcatttgttg agccttagat aaggtaagaa gatgctcaac
    79441 aatatgctca agacgcaagc tttctttgta aataatgtct agaaagtcat ccttgagcgc
    79501 ttcttcttca gctgacatcc ccttaatggt ttcagcaaag cccttaattg aagtaactgg
    79561 tgtcctcaat tcatgggagg catttgagac aaaggctaaa tttaactttt cataagttct
    79621 aatcgttgtt aaatcatata gcaagacgag cacagcttcc acagattggg tggggctaaa
    79681 aacgggaact gctgtcactt ctaaaatcaa gtcaccctca tgaaacccac ttacttcttg
    79741 ttttaacctt gttttttgat caaaggcttg gtgaactaaa ttccgaatat ccatccgttt
    79801 gaggtcatca agtgaactta tgtcgccgtc cacatcggga aaataatgag gcagagagcg
    79861 actggataat aacatctgac cttgagcgga aactaaaaac gtccccatgg ttaggtgcga
    79921 cagaagaacc tccattgttt cggctaggtc cttgtattgc tgatcctgtt gggagacttt
    79981 ggtttttagg ccagacacat acttagccaa agactttaag tcttcttgcc ctttttctaa
    80041 aaagtattca ctactggtca agagaggttg gtgcaaggtc tcaaaagcaa cttcccattt
    80101 ccaaaggcaa aagagccagt agccacctag tcccaaagaa agggctagaa gaaagagacc
    80161 gatgccttta ctgatccaag ttaatgccat tcctgcaatc agaatgaggc taacacttag
    80221 attgactagc caaaattgaa ggtagcgttt catctataac tccttgaact tataaccata
    80281 accccgaatg gttcgaataa attgaggggc tttaggattg tcttcaattt tttccctcaa
    80341 cttaccaata tgaacgtcca ccaaacgtgt ttcctgccca aagtcatatc cccagatacg
    80401 ttccaaaaga cgctctctag tcagtgtcat gttgggatgt ttcataagat agagcaagag
    80461 ttcaaattct tttggggtca aactcagtaa cttattcgcc ttgtagacct catgacgctc
    80521 agggtatact ttcaaggtcc caaatagcca agaatcgtca gcaatattat ctgaatcatc
    80581 tccttcttgt tctcctttag ttcgcctgag gacagccttg acacgtgcca gcaattctct
    80641 agggctaaaa ggcttggtca ggtagtcatc agcccctaat tccaaggcca aaaccttatc
    80701 aaattcatca cttttcgcag aaaccatcat aattggagtt ttgatgcctt tggctctcag
    80761 ccgcttacaa acttctatgc catctaattg tggtaacatg atatcaagca agataaaatc
    80821 aaagggttct gtttctgcca aagctaaggc cttccgtcca tttgttacca attgagtaga
    80881 aaagccttcc ttacttaaat ggtagtcaag caatctcaga atgtgttctt catcatccac
    80941 taataagact tgttttgtca tctattatct cctattggta acattataac acaattatca
    81001 gaaatcctaa cattgctaaa tcagattaaa tttgcttatc aagactagta tctggtcaaa
    81061 tgctcaatca tctccttgtg ctctggatag gtcgccagta gatctgccct ttcaaataat
    81121 tcaaaatcct caaattcaaa accaggagca acaagacaag aaaccagagc ataatcctta
    81181 tcaactgttg atccccaaat agtgccctta ggaacacagt agtgaagttg ttgccctttg
    81241 gatatgtcca ggcctaaagt gactgcttcg tagtgaccat ctgctgtaat catgtgaaca
    81301 gtaagtgggg atcctgcatg aaaataccag atttcatctg ctgtcaatcg gtgaaaatgt
    81361 gaaggattcg tttcttctaa taagaaataa atactggtat aaagcgccct tcctttacca
    81421 gcaaggttta tagtgtctga agcttttttt gtttgtctaa aatagccacc ttcaacatgg
    81481 ggagctaact ctagagttct tatcaagtct tctttatccg tcggagccaa tgggttgaag
    81541 taactcttgt tcaaagtagt tttacgattt caagaactcc tctcagttct gaggacacgg
    81601 taatgattga tgcgacggaa gtaaaaatca atcgccctaa aaaagaatta gcgaatgatt
    81661 ctggtaaaaa gaaatgccac gctatgaagg ctcaagcgat tgtcacaagt caagggagaa
    81721 ttgtttcttt ggatatcact gtgaactatt gtcatgatat gaagttgttc aaaatgagtc
    81781 gcagaaatat cggacaagct ggtaaaatct tggctgacag tggttatcaa gggcttatga
    81841 agatatatcc tcaagcacaa actccacgta aatccagcaa actcaagccg ctaacagctg
    81901 aagataaagc ctgtaaccat gcactatcta aggagagaag caaggttgag aacatctttg
    81961 ccaaagtaaa aacgtttaaa atgttttcaa caacttatcg aaatcatcgt aaacgcttcg
    82021 gattacgaat gaatttgatt gctggcatta tcaatcatga actaggattc tagttttgca
    82081 ggaagtctat tatttggtta ggtgaattag tgaagcgttt aggcaagtgt ctctggttac
    82141 gacgtaaaga agcataaatc gataatattt atgtttcttt actagtgaaa cgcctagcca
    82201 aattaccata gttacgacgt catggcctct aaatcgatta tatttagggg tcatgactag
    82261 tgaagcagtt agctagttcg catataagcg gctagcgtct aacaattagg aactttagtt
    82321 ccaataactt taagattacg acgttttagg acataaatcg atcatattta tgtcctaaaa
    82381 ctagtgaagc gcctagccaa agtccgaata ggatttggcg ttagttactt agattgcttt
    82441 gcaatcaagt aactttggcg atttacatct tctctggcgc ttctactcca agcaagcgaa
    82501 gggcttcttt gagaacgact gcggttgcgt agctgagggc tagacggctg tcgcgttctg
    82561 ggctttcatc caagatacgt gtatgtgcat agtatttgtt aaaggattga gctaggctaa
    82621 ttgcaaattt agcaatgata gaaggttcaa agttatctgc cgcacggttg ataatacgtg
    82681 ggaagtcttg aatgagttta atgatttccc agctttcagt atcattcaag ctatagttgc
    82741 cagctgtttc tggtttgaaa tcggctttgc gtaagataga ttggatacga gcgtaggcat
    82801 attgaacgta aggtccagtt tcaccctcga aggataccat agcctctagg tcgaagtcgt
    82861 atccatttgt acggtcggtt ttgaggtcat agaatttaat ggctccaacc ccaacagcat
    82921 gtgctacttg gtctttgttt tctagttcag gatttttagc ctcgatttgg accttggcac
    82981 ggctaacagc ctctgcaaca gtaggctcta gcaagatgac attcccttta cgagtagaga
    83041 gtttcttccc ttcttttgta accaaaccaa aaggaacgtg agtaatgtcg tcactccagt
    83101 cgtagcccat ctcttgcaag acagctttga gctgtttaaa gtgggcagat tgttcttgac
    83161 caacgacata gatagattta gcaaattggt attcgttttt acggtaaagg gctgcagcca
    83221 agtcacgtgt gatatagaga gttgcaccat cagatttctt gatgagagct ggatgttcaa
    83281 ttccgtattt ctcaagattg acaacttggg caccttctga ttcaagaagt agtccttttt
    83341 cagaaagaat gtctacaact gcatccatct tatcattgta gaaggcttct ccgttatagc
    83401 tgtcaaattc aaccttcaat tcattgtaaa ggcggttaaa ttccactaaa ctttcatcgc
    83461 ggaaccattg ccaaagagcg agagcttcct catctccatt ttcaagttta cggaaccatt
    83521 cgcgcgcttc ttcatccaag ctagggtcat tttcagcttc agcgttgatg cggacataga
    83581 gtttaaggag ttcatcgatt ggatgagctt ttacagcttc ttcgtcgccc cattttttgt
    83641 aggcaacaat caacatccca aattgtttac cccagtctcc caaatggttg accttgaccg
    83701 tttgataacc gattttttgg aaaatatgtg acaagctatc tccgataaca gttgaacgca
    83761 ggtggccaat agaaaatggt ttagcgatat tcggactaga catgtcgata acaacatttt
    83821 cttgtttacc aatattttgg tcagcatagt gttctttttc agtggtaaca gcttgcaata
    83881 cttgagcaga aatggcagat ttatcaagga aaaagttaac gtaaggtcct gttgcgacaa
    83941 ctttttcaaa ggcttggctg ttcatttttt cagccagttc agccgcaatc atttgtggtg
    84001 ctttacgttc gactttggca agagaaaaag cagggaaagc gatgtcaccc atttctgagt
    84061 ttttaggggt ttccagtaac tttaaaatag cctcttggtc caggctatca atgatgctag
    84121 ccaattcgct agcaatcaat tcttttgtat tcattaagag ctcctttttg gacttttcta
    84181 ctattttatc acaattttaa agaaagaaga aaaaattttt gaaatctcct gtttttttgg
    84241 tataatatgg ttataaatat agttataaat atagttataa atatgcacgc aagaggattt
    84301 tatgagaaaa agagatcgtc atcagttaat aaaaaaaatg attactgagg agaaattaag
    84361 tacacaaaaa gaaattcaag atcggttgga ggcgcacaat gtttgtgtga cgcagacaac
    84421 cttgtctcgt gatttgcgcg aaatcggctt gaccaaggtc aagaaaaatg atatggtgta
    84481 ttatgtacta gtaaatgaga cagaaaagat tgatttggtg gaatttttgt ctcatcattt
    84541 agaaggtgtt gcaagagcag agtttacctt ggtgcttcat accaaattgg gagaagcctc
    84601 tgttttggca aatattgtag atgtaaacaa ggatgaatgg attttaggaa cagttgctgg
    84661 tgccaatacc ttattggtta tttgtcgaga tcagcacgtt gccaaactca tggaagatcg
    84721 tttgctagat ttgatgaaag ataagtaagg tcttgggagt tgctctcaag acttattttt
    84781 gaaaaggaga gacagaaaat ggcgatagaa aagctatcac ccggcatgca acagtatgtg
    84841 gatattaaaa agcaatatcc agatgctttt ttactctttc ggatgggtga tttttatgaa
    84901 ttattttatg aggatgcggt caatgctgcg cagattctgg aaatttcctt aacgagtcgc
    84961 aacaagaatg ccgacaatcc gatccctatg gcgggtgttc cctatcattc tgcccaacag
    85021 tatatcgatg tcttgattga gcagggttat aaggtggcta tcgcagagca gatggaagat
    85081 cctaaacaag cagttggggt tgttaaacga gaggttgttc aggtcattac gccagggaca
    85141 gtggtcgata gcagtaagcc ggacagtcag aataattttt tggttgccat agaccgcgaa
    85201 ggcaatcaat ttggtctagc ttatatggat ttggtgacgg gtgactttta tgtgacaggt
    85261 cttttggatt tcacgctggt ttgtggggaa atccgtaacc tcaaggctcg agaagtggtg
    85321 ttgggttatg acttgtctga ggaagaagaa caaatcctca gccgccagat gaatctggta
    85381 ctctcttatg aaaaagaaag ctttgaagac cttcatttat tggatttgcg attggcaacg
    85441 gtggagcaaa cggcatctag taagctgctc cagtatgttc atcggactca gatgagggaa
    85501 ttgaaccacc tcaaacctgt tatccgctac gaaattaagg atttcttgca gatggattat
    85561 gcgaccaagg ctagtctgga tttggttgag aatgctcgct caggtaagaa acaaggcagt
    85621 cttttctggc ttttggatga aaccaaaacg gctatgggga tgcgtctctt gcgttcttgg
    85681 attcatcgcc ccttgattga taaggaacga atcgtccaac gtcaagaagt ggtgcaggtc
    85741 tttctcgacc atttctttga gcgtagtgac ttgacagaca gtctcaaggg tgtttatgac
    85801 attgagcgct tggctagtcg tgtttctttt ggcaaaacca atccaaagga tctcttgcag
    85861 ttggcgacta ccttgtctag tgtgccacgg attcgtgcga ttttagaagg gatggagcaa
    85921 cctgctctag cctatctcat tgcacaactg gatgcaatcc ctgagttgga gagtttgatt
    85981 agcgcagcga ttgctcctga agctcctcat gtgattacag atgggggaat tatccggact
    86041 ggatttgatg agactttaga caagtatcgt tgcgttctca gagaagggac tagctggatt
    86101 gctgagattg aggctaagga gcgagaaaac tctggtatca gcacgctcaa gattgactac
    86161 aataaaaagg atggctacta ttttcatgtg accaattcgc aactgggaaa tgtgccagcc
    86221 cactttttcc gcaaggcgac gctgaaaaac tcagaacgct ttggaaccga agaattagcc
    86281 cgtatcgagg gagatatgct tgaggcgcgt gaggagtcag ccaacctcga atacgaaata
    86341 tttatgcgca ttcgtgaaga ggtcggcaag tacatccagc gtttacaagc tctagcccaa
    86401 ggaattgcga cggttgatgt cttacagagt ctggcggttg tggctgaaac ccagtatttg
    86461 attcgacctg agtttggtga cgattcacaa attgatattc ggaaagggcg ccatgctgtc
    86521 gttgaaaagg ttatgggggc tcagacctat attccaaata cgattcagat ggcagaagat
    86581 accagtattc aactgattac agggccaaac atgagtggga agtctaccta tatgcgtcag
    86641 ttagccatga cggcggttat ggcccagctg ggttcctatg ttcctgctga aagcgcccat
    86701 ttaccgattt ttgatgcgat ttttacccgt atcggagcag cagatgactt ggtttcgggt
    86761 cagtcaacct ttatggtgga gatgatggag gccaataatg ccatttcgca tgcgaccaag
    86821 aactctctca ttctctttga tgaattggga cgtggaactg caacttatga cgggatggct
    86881 cttgctcagt ccatcatcga atatatccat gagcacatcg gagctaagac cctctttgcg
    86941 acccactacc atgagttgac tagtctggag tctagtttac aacacttggt caatgtccac
    87001 gtggcaactt tggagcagga tgggcaggtc accttccttc acaagattga accgggacca
    87061 gctgataaat cctacggtat ccatgttgcc aagattgctg gcttgccagc agacctttta
    87121 gcaagggcgg ataagatttt gactcagcta gagaatcaag gaacagagag tcctcctccc
    87181 atgagacaaa ctagtgctgt cactgaacag atttcactct ttgatagggc agaagagcat
    87241 cctatcctag cagaattagc taaactggat gtgtataata tgacacctat gcaggttatg
    87301 aatgtcttag tagagttaaa acagaaacta taaaaccaag actcactagt taatctagct
    87361 gtatcaagga gacttctttg acaattctcc acttttttgc tagaataaca tcacacaaac
    87421 agaatgaaaa ggagctgacg cattgtcgct cccttttgtc tattttttaa ggagaaagta
    87481 tgctgattca gaaaataaaa acctacaagt ggcaggccct ggcttcgctc ctgatgacag
    87541 gcttgatggt tgctagttca cttctgcaac cgcgttatct gcaggaagtc ttaggcgccc
    87601 tccttactgg gaaatatgaa gctatttata gtatcggggc ttggttgatt ggtgtggccg
    87661 tagtcggtct agttgctggt ggactcaatg ttgtcctcgc agcctatatt gcccaaggag
    87721 tttcatccga ccttcgggag gatgccttcc gtaaaattca aaccttttct tatgctgata
    87781 ttgaacaatt taatgcggga aatctagtcg ttcgaatgac aaatgatatc aaccagattc
    87841 agaacgttgt catgatgacc ttccaaattc ttttcagact tcccctcttg ttcatcggtt
    87901 cgtttatcct agcggttcaa accttacctt ctctgtggtg ggtgattgtt ctcatggtag
    87961 tcttgatttt tggtttgact gctgtcatga tgggaatgat ggggcctcgt tttgctaaat
    88021 ttcaaaccct tcttgagcgc atcaatgcca ttgccaagga aaatttacgt ggcgttcgtg
    88081 tggtcaagtc ctttgtccaa gaaaaagagc aatttgctaa gtttacagag gtctcaaacg
    88141 agcttcttgg tcaaaacctt tacattggtt atgccttttc agtagtggaa ccctttatga
    88201 tgttggttgg ttacggggcg gtcttcctct ctatttggct ggtcgcggga atggttcagt
    88261 cggatccgtc tgttgttggt tccatcgctt cttttgttaa ttacctaagc cagattatct
    88321 ttaccattgt tatggttgga tttttgggaa attctgtcag ccgtgccatg atttccatgc
    88381 gtcgtattcg agaaattctt gacgcagagc cagctatgac cttcaaggat atcccagatg
    88441 aagagttggt tggaagtctt agctttgaaa atgtgacctt tacctatcca atggacaagg
    88501 aaccgatgct gaaagatgtg agctttacta ttgaacctgg tcaaatggtt ggtgtagttg
    88561 gagcgactgg tgcaggaaag tcaaccttgg ctcaattgat tccacgtctc tttgatccac
    88621 aggacggggc cattaaaatc ggtggcaagg atattcgaga agtgagtgaa ggaaccctgc
    88681 gtaaaacagt ttccatcgtt ctccaacgtg ccattctttt tagtggaacg attgcagata
    88741 acttgagaca ggggaaggga aatgctactc tatttgaaat ggagcgcgca gccaatattg
    88801 cccaggctag tgaattcatt catcgtatgg agaaaacctt tgaaagtcca gttgaagaac
    88861 ggggaaccaa tttctctggt ggacaaaaac aaaggatgtc gattgcgcgt gggattgtca
    88921 gcaatccacg tattctgatt tttgacgatt cgacctcagc cttggatgcc aaatcagagc
    88981 gcttggtgca agaagctttg aataaggact tgaaggggac gacaaccatt attattgctc
    89041 aaaaaattag ctcggttgtc catgcagaca agatcttggt tctaaatcaa ggacgattga
    89101 ttggtcaagg cacgcatgca gacttggttg ccaacaatgc cgtttaccgt gaaatctatg
    89161 aaacacagaa atgaaagaca aactataaga aaagtcaata gttttatcta aactatttct
    89221 tatttcaatt tgatgatttg gcgatgattt tagagcacgg caaaaagccc ttgaaaaagt
    89281 ccattttttc aaaggtaatc ctgtgttgat ttcagaaatt acatcacttt ttgttcgtca
    89341 aatggcagct cttttttagg atataaaaca gggttcggat aagttttttt gcaaggtgga
    89401 tgatggctac attgtaatgt tttccttgtt ctaacttagt cttaaaagca ggtgaaaagt
    89461 gagggcatgc tttggcagct tgtatgagta cctaccgcag atgaggggaa ccccgtttga
    89521 ccattcttcc agctaaatca atctgacctg actgataaat agaagaatcc agtccagcga
    89581 aagcttgtaa ttgagcagga ttatcaaagg catgaatatt tcgaatctcg gctaaaatga
    89641 ccgcccctaa acgattctca atcccagtaa ccgtcgtgat gaccgagttt aactcagcca
    89701 tcaagtcatt gacacatttt tccgccttgt caatgagcct cttgtaatgt ttgatgtttt
    89761 cattacacga gataaaacgt ctatgcgtta tcaaactcat taccaattaa aacaaatgtg
    89821 gttagatcct ttcggaaatt gtcaagcgat tggaggaaat gaactaatcc acagcggctt
    89881 attccaagta taccacttgg gctttggcag tagctaactg cgctaaatat aatataagga
    89941 ggagtaaaat gaagacagtt caattttttt ggcattattt taaggtctac aagttctcat
    90001 ttgtagttgt catcctgatg attgttctgg cgacttttgc ccaagccctc tttccagtct
    90061 tttctggaca agcggtgacg cagctagcca atttagttca agcttatcaa aatggcaatc
    90121 cagaacttgt atggcaaagc ctatcaggaa tcatggtcaa tcttggcctg ctggttttgg
    90181 ttctatttat ctctagtgta atatacatgt gtctcatgac gcgcgtgatt gcagaatcga
    90241 ccaacgagat gcgcaaaggt ctctttggta agcttgctca gttgacggtt tctttctttg
    90301 accgtcgaca agatggcgat atcctgtctc attttaccag tgatttggat aatatcctcc
    90361 aagcctttaa cgaaagcttg attcaggtca tgagcaatat tgttttatac attggtctga
    90421 ttcttgtcat gttttcgaga aatgtgacgc tggctctcat caccattgcc agcaccccat
    90481 tggctttcct tatgctgatt ttcatcgtga aaatggcacg taaatacacc aacctccagc
    90541 agaaagaggt agggaagctc aacgcctata tggatgagag catctcaggc caaaaagccg
    90601 tgattgtgca aggaattcaa gaggatatga tggcaggatt tcttgaacaa aatgagcgcg
    90661 tgcgcaaggc aacctttaaa ggaagaatgt tctcaggaat tcttttccct gtcatgaatg
    90721 ggatgagcct gattaataca gccatcgtca tctttgctgg ttcggctgta cttttgaatg
    90781 ataagtctat tgaaacaagt acagccctag gtttgattgt tatgtttgca caattttcac
    90841 agcagtacta ccagcctatt atccaagttg cagcgagttg gggaagcctt cagttggcct
    90901 ttactggagc tgaacgaatt caggaaatgt ttgatgcaga ggaggaaatc cgacctgaaa
    90961 aggctccaac cttcactaag ttgcaagaaa gtgttgaaat cagtcatatc gatttttcat
    91021 acttgcctga taaacctatt ttgaaagatg tcagcatttc tgcccctaaa ggccagatga
    91081 cagcagttgt tgggccgaca ggttcaggaa aaacgactat tatgaacctc atcaatcgct
    91141 tttatgatgt tgatgctggt ggtatttatt ttgatggtaa agacattcgt ggctatgact
    91201 tagatagtct tagaagcaag gtgggaattg tattgcaaga ttcggtcttg tttagcggaa
    91261 cgattcgaga caatatccga tttggtgtgc cagatgctag tcaggaaatg gttgaggtag
    91321 cagcaaaagc aacccacatt cacgactata tcgaaagttt gcctgataag tacgatactc
    91381 ttattgatga tgaccagagc atcttttcaa cagggcagaa gcaattgatt tcaatcgctc
    91441 gaaccctgat gacagatcca gaagttctca ttctcgatga agcaacttca aacgtagata
    91501 cggtgacaga aagcaagatt cagcatgcca tggaggtggt tgtagcaggt agaactagtt
    91561 tcgtcattgc ccaccgcttg aaaaccattc tcaatgcaga tcagattatt gtccttaaag
    91621 atggagaagt cattgaacgt ggtaaccacc atgaactttt gaagctaggt ggcttttatt
    91681 cagaactcta tcacaatcaa tttgttttcg aataagaaag aagttgtcct atgtgggcag
    91741 ctttttcttg tccataaaaa atgtttatca cagccttaaa aaaaacatat tagacgaaag
    91801 tcattttgag tgatatgata ggactatcgt tagcattcga aaggagaggc atcatggcta
    91861 gaacggttgt aggagttgct gcaaatctat gtcccgtaga cgcagaaggc aaaatcattc
    91921 attcatctgt atcttgtaga ttcgcagaga tcattcgtca agtcggtggt ctccctttag
    91981 tcattcctgt tggtgatgag tcagttgtac gtgattatgt ggaaatgatt gacaaactca
    92041 ttttgacagg aggccaaaat gttcatcctc agttttatgg agagaaaaag accgtcgaga
    92101 gcgatgatta caatctggtc cgtgacgaat ttgaattggc actcttgaag gaagcgcttc
    92161 gtcagaataa accaattatg gcaatctgtc gcggtgtcca acttgtcaat gttgcctttg
    92221 gtggaaccct caatcaagaa atcgaaggtc actggcaagg actacctttc gggacatatc
    92281 actctattga gacagtagaa ggaagcgtgg tggctaagct ctttgggaaa gaaagtcagg
    92341 tcaactcagt ccatcgtcaa agcattaaag atttggcacc taatttccgt gtaactgcta
    92401 ttgattcaag agaccaaact attgaagcga ttgagtctat cgacgagcac cgcattatcg
    92461 gtttacagtg gcatccagag tttctggtta atgaagaaga tggcaattta gaattatttg
    92521 agtatttatt gaatgaactg taacgattgg aacatctggt cgttctttct tttatttttt
    92581 caaaattttt agaatgtggt attttacgca aacgtttgaa ttctgataaa aattggagaa
    92641 attcgacaag aaaacttgaa aaaaacgaag gtaagcgtta tgatagaaaa gaagaaatat
    92701 tggaggaaga acatgtcaca tattaaattt gattattcaa aagttttaga caaatttgtt
    92761 gcaccacatg aagtggaata catgcaatca caagtaacag cagcagatga attgatccgt
    92821 aaaggaactg gtgctggtag cgacttcttg ggatggttgg accttcctga aaaatacgat
    92881 cgcgaagaat ttgaccgcat cttgaaagct gctgagcaaa tcaagtcaga cagcgatgtt
    92941 ttggttgtga tcggtattgg tggatcttac cttggtgcaa aagcagcaat cgacttcttg
    93001 aatcaccatt ttgctaactt gcaaacaaaa gaagaacgca aagctccaca aatcctttac
    93061 gcaggaaact caatctcatc tacttacctt gctgacttgg tagagtatgt tgcagacaaa
    93121 gacttctcag taaacgtaat ttctaaatca ggtacaacaa ctgaaccagc gattgctttc
    93181 cgtgtcttta aagaactctt ggttaagaaa tacggtcaag aagaagctaa caaacgtatc
    93241 tatgcaacaa ctgaccgcca aaagggtgct gttaaggttg aagcagacgc taacggttgg
    93301 gaaacatttg ttgttccaga tgatatcggt ggacgcttct cagtattgac agccgttggt
    93361 ttgcttccaa tcgcagcatc aggagctgac atcaaagctc ttatggaagg tgcgaatgca
    93421 gctcgcaaag actacacttc agacaaaatc tctgaaaacg aagcttacca atacgcagct
    93481 gttcgtaaca tcctttatcg taaaggctat gcaactgaga tcttggtaaa ctatgagcca
    93541 tcacttcaat acttctcaga atggtggaaa caattggctg gtgaatcaga aggaaaagac
    93601 aaaaaaggta tctacccaac ttcagccaac ttctcaactg acttgcactc acttggtcaa
    93661 tttatccaag aaggaactcg tatcatgttt gaaacagttg tccgtgttga caaacctcgt
    93721 aaaaacgtgc ttattcctac tttggaagaa gaccttgacg gacttggtta ccttcaagga
    93781 aaagacgttg actttgtaaa caaaaaagca actgacggtg ttcttcttgc ccacacagat
    93841 ggtgatgtac caaacatgta tgtgactctt ccagagcaag acgctttcac tcttggttac
    93901 actatctact tcttcgaatt ggcaattgcc ctttcaggtt acttgaatgc tatcaaccca
    93961 tttgaccaac caggtgttga agcttataaa cgtaacatgt ttgcccttct tggaaaacca
    94021 ggatttgaag aattgagcaa agaacttaac gcacgtctat aatagaagaa aagagtggtt
    94081 tgcccactct ttttactctc tttatccata gaaattggac tcagccaaga cttgtgatat
    94141 aatatagaaa gcaaaaaggc agacgcctag ataataggag aaactatgtc aaaagatatc
    94201 cgcgtacgtt acgcaccaag tccaacagga ctactacaca tcggaaatgc ccgtacagca
    94261 ttgtttaact acttgtatgc gcgccatcat ggtggaacat ttctcatccg tatcgaagat
    94321 actgaccgta aacgccatgt cgaggatggt gaacgttcac aacttgaaaa ccttcgctgg
    94381 ttaggcatgg attgggatga aagtccagaa tcacatgaga attatcgcca gtctgagcgt
    94441 ttggacttgt atcaaaaata cattgaccaa ctattagctg aaggaaaagc ctataaatct
    94501 tacgttacag aagaagagtt ggcagctgaa cgtgaacgtc aagaagctgc tggtgaaaca
    94561 cctcgatata tcaacgaata ccttggtatg agtgaagagg aaaaagcggc ttatatcgca
    94621 gaacgtgaag cagctggtat cataccaaca gttcgtttgg ctgtcaatga gtcaggtatc
    94681 tacaagtggc atgatatggt caaaggtgat atcgaatttg aaggtggcaa tatcggcggt
    94741 gactgggtta tccaaaagaa agacggttac ccaacttaca actttgccgt tgttatcgat
    94801 gaccacgata tgcaaatctc tcatgttatc cgtggagatg accatattgc taatacacca
    94861 aaacagctta tggtctatga agctcttggt tgggaagctc cagagtttgg tcacatgacc
    94921 ttgattatca actctgagac tggtaagaag ttatctaaac gtgatactaa tacacttcaa
    94981 tttatcgaag attaccgaaa gaaaggttac cttccagaag cagtctttaa ctttattgct
    95041 cttcttggtt ggaacccagg tggtgaagat gagattttct ctcgtgaaga actcattaaa
    95101 ctttttgatg aaaaccgcct cagcaagtca ccagcagcct ttgatcagaa aaaactagac
    95161 tggatgagca atgattatat caagaatgca gaccttgaaa ccatctttga aatggcaaaa
    95221 ccattcttag aggaagcagg ccgtttgact gacaaggctg aaaaattagt tgagctctat
    95281 aaaccacaaa tgaaatcagt agatgagatt atcccattga cagatctttt cttctcagat
    95341 ttcccagaat tgacagaagc agagcgcgaa gtcatgacgg gtgaaacagt tccaacagtt
    95401 cttgaagcat tcaaagcaaa acttgaagcg atgacagatg atgaatttgt gacagaaaat
    95461 atcttcccgc aaattaaagc agtccaaaaa gaaacaggta ttaaagggaa aaatcttttc
    95521 atgcctattc gtatcgcagt ttcaggcgaa atgcatgggc cagaattacc agatacaatt
    95581 ttcttgcttg gacgtgaaaa atcaattcag catatcgaaa acattctaaa agaaatctct
    95641 aaataaagag gatacaataa tggacatctg ggaaaagatg tacgaagaag cacagaaact
    95701 atacaatcca catgaagtat cagactttgt ttatgccaat catgttgtag ctgcagtaga
    95761 agcaggagat ggacaaatat ttacaggatt ctgtatggag ggaacctgtg gtgttttcca
    95821 tctctgcgca gaacgggcgg ccctcttcaa tatgtaccaa ttttcaggac aaactaaggt
    95881 taagaaagta ttagcctttc gagacaaacc accttatggt ggaagttcag ccatgccttg
    95941 cggtgcttgt agagaattcc ttttagagct taatgctgaa aataaagatg cagaattcat
    96001 gatggactat aatataagaa aaacagttaa agtcgcagaa ctaatccctt attggtgggg
    96061 aaaagaacgt gcttctaagt ttaatgagcg atagaagagt cagtataatt ctggctcttt
    96121 ttactataag
//
LOCUS       DAVKAO010000002        67838 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKAO010000002 DAVKAO010000000
VERSION     DAVKAO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.002"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            313..438
                     /locus_tag="VWX16_000088"
     CDS             313..438
                     /locus_tag="VWX16_000088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9157532.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELG"
     gene            442..1176
                     /locus_tag="VWX16_000089"
     CDS             442..1176
                     /locus_tag="VWX16_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEU9157533.1"
                     /translation="MLSADKLILAIQGINVTLDEFGHKLNNYQESPHMRIGRKVVNRF
                     AHQDIAALEQLLEEVDQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLY
                     AIESWTWFELYLFCNTMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILS
                     ELMERKLFSYIPIFEAELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQ
                     SLQVLENPQLTSLFELRFQQYKELID"
     gene            1467..1664
                     /locus_tag="VWX16_000090"
     CDS             1467..1664
                     /locus_tag="VWX16_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9157534.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWL
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            1732..2396
                     /locus_tag="VWX16_000091"
                     /pseudo
     CDS             1732..2396
                     /locus_tag="VWX16_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            2468..3694
                     /locus_tag="VWX16_000092"
     CDS             2468..3694
                     /locus_tag="VWX16_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9157535.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADTSFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSAFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLKHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(4045..4122)
                     /locus_tag="VWX16_000093"
                     /pseudo
     CDS             complement(4045..4122)
                     /locus_tag="VWX16_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4193..4849
                     /locus_tag="VWX16_000094"
     CDS             4193..4849
                     /locus_tag="VWX16_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9157536.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQMMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            4839..5162
                     /locus_tag="VWX16_000095"
     CDS             4839..5162
                     /locus_tag="VWX16_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9157537.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMISFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            5266..6096
                     /locus_tag="VWX16_000096"
     CDS             5266..6096
                     /locus_tag="VWX16_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9157538.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            6250..7104
                     /locus_tag="VWX16_000097"
     CDS             6250..7104
                     /locus_tag="VWX16_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9157539.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
     gene            7204..8577
                     /locus_tag="VWX16_000098"
     CDS             7204..8577
                     /locus_tag="VWX16_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9157540.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAYFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQRHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEDRMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            8570..9631
                     /locus_tag="VWX16_000099"
     CDS             8570..9631
                     /locus_tag="VWX16_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9157541.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEESSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            9633..10325
                     /locus_tag="VWX16_000100"
     CDS             9633..10325
                     /locus_tag="VWX16_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9157542.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIVIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(10355..10924)
                     /locus_tag="VWX16_000101"
     CDS             complement(10355..10924)
                     /locus_tag="VWX16_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9157543.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            11059..11673
                     /locus_tag="VWX16_000102"
     CDS             11059..11673
                     /locus_tag="VWX16_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9157544.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFISSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            11675..13321
                     /locus_tag="VWX16_000103"
     CDS             11675..13321
                     /locus_tag="VWX16_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9157545.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKVSPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTEPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            13333..14619
                     /locus_tag="VWX16_000104"
     CDS             13333..14619
                     /locus_tag="VWX16_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9157546.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATVSHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQAWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRIPITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            14671..15204
                     /locus_tag="VWX16_000105"
     CDS             14671..15204
                     /locus_tag="VWX16_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9157547.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            15282..15752
                     /gene="nrdI"
                     /locus_tag="VWX16_000106"
     CDS             15282..15752
                     /gene="nrdI"
                     /locus_tag="VWX16_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9157548.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEIDHPYVAFLPTYLEGGNGVDSGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(16045..17307)
                     /locus_tag="VWX16_000107"
     CDS             complement(16045..17307)
                     /locus_tag="VWX16_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9157549.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSTM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIIIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(17617..18075)
                     /locus_tag="VWX16_000108"
     CDS             complement(17617..18075)
                     /locus_tag="VWX16_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9157550.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(18072..18512)
                     /locus_tag="VWX16_000109"
     CDS             complement(18072..18512)
                     /locus_tag="VWX16_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9157551.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            18868..20817
                     /gene="mutL"
                     /locus_tag="VWX16_000110"
     CDS             18868..20817
                     /gene="mutL"
                     /locus_tag="VWX16_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9157552.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(21142..21609)
                     /locus_tag="VWX16_000111"
     CDS             complement(21142..21609)
                     /locus_tag="VWX16_000111"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9157553.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(21610..22815)
                     /locus_tag="VWX16_000112"
     CDS             complement(21610..22815)
                     /locus_tag="VWX16_000112"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9157554.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(22835..23470)
                     /gene="ribE"
                     /locus_tag="VWX16_000113"
     CDS             complement(22835..23470)
                     /gene="ribE"
                     /locus_tag="VWX16_000113"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9157555.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(23455..24555)
                     /gene="ribD"
                     /locus_tag="VWX16_000114"
     CDS             complement(23455..24555)
                     /gene="ribD"
                     /locus_tag="VWX16_000114"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9157556.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAIIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     SNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(24689..24803)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            24960..25553
                     /gene="ruvA"
                     /locus_tag="VWX16_000115"
     CDS             24960..25553
                     /gene="ruvA"
                     /locus_tag="VWX16_000115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9157557.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            25563..26126
                     /locus_tag="VWX16_000116"
     CDS             25563..26126
                     /locus_tag="VWX16_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9157558.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            26123..26800
                     /locus_tag="VWX16_000117"
     CDS             26123..26800
                     /locus_tag="VWX16_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9157559.1"
                     /translation="MMSNKNKGILIFVILYTVLFVFDGVKLLASLMPFAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(26955..27986)
                     /locus_tag="VWX16_000118"
     CDS             complement(26955..27986)
                     /locus_tag="VWX16_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9157560.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKHLGLNPIFLSHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIKTGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(28241..28924)
                     /locus_tag="VWX16_000119"
     CDS             complement(28241..28924)
                     /locus_tag="VWX16_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9157561.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(28936..29880)
                     /locus_tag="VWX16_000120"
     CDS             complement(28936..29880)
                     /locus_tag="VWX16_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9157562.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            30012..32843
                     /gene="uvrA"
                     /locus_tag="VWX16_000121"
     CDS             30012..32843
                     /gene="uvrA"
                     /locus_tag="VWX16_000121"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9157563.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHG"
     gene            32836..33897
                     /locus_tag="VWX16_000122"
     CDS             32836..33897
                     /locus_tag="VWX16_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9157564.1"
                     /translation="MDKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            34029..34427
                     /gene="spx"
                     /locus_tag="VWX16_000123"
     CDS             34029..34427
                     /gene="spx"
                     /locus_tag="VWX16_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9157565.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            34493..35062
                     /locus_tag="VWX16_000124"
     CDS             34493..35062
                     /locus_tag="VWX16_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9157566.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSNYISDRLANGATEQ"
     gene            35149..35415
                     /locus_tag="VWX16_000125"
     CDS             35149..35415
                     /locus_tag="VWX16_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9157567.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            35419..35838
                     /gene="ruvX"
                     /locus_tag="VWX16_000126"
     CDS             35419..35838
                     /gene="ruvX"
                     /locus_tag="VWX16_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9157568.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            35854..36159
                     /locus_tag="VWX16_000127"
     CDS             35854..36159
                     /locus_tag="VWX16_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9157569.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            36466..37716
                     /locus_tag="VWX16_000128"
     CDS             36466..37716
                     /locus_tag="VWX16_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9157570.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVQGYLLDREQIN"
     gene            37713..38231
                     /locus_tag="VWX16_000129"
     CDS             37713..38231
                     /locus_tag="VWX16_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9157571.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTGQDDG
                     DRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIVVDDM
                     DRDPSDQIVLTK"
     gene            38377..39909
                     /gene="cls"
                     /locus_tag="VWX16_000130"
     CDS             38377..39909
                     /gene="cls"
                     /locus_tag="VWX16_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9157572.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGINLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            39987..40130
                     /locus_tag="VWX16_000131"
     CDS             39987..40130
                     /locus_tag="VWX16_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157573.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            40167..41705
                     /locus_tag="VWX16_000132"
     CDS             40167..41705
                     /locus_tag="VWX16_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9157574.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVEVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            41825..44032
                     /gene="nrdD"
                     /locus_tag="VWX16_000133"
     CDS             41825..44032
                     /gene="nrdD"
                     /locus_tag="VWX16_000133"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9157575.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            44044..44184
                     /locus_tag="VWX16_000134"
     CDS             44044..44184
                     /locus_tag="VWX16_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157576.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            44228..44734
                     /locus_tag="VWX16_000135"
     CDS             44228..44734
                     /locus_tag="VWX16_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9157577.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            44727..45317
                     /gene="nrdG"
                     /locus_tag="VWX16_000136"
     CDS             44727..45317
                     /gene="nrdG"
                     /locus_tag="VWX16_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9157578.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGILYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            45314..45931
                     /locus_tag="VWX16_000137"
     CDS             45314..45931
                     /locus_tag="VWX16_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9157579.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            46197..46505
                     /gene="rpsJ"
                     /locus_tag="VWX16_000138"
     CDS             46197..46505
                     /gene="rpsJ"
                     /locus_tag="VWX16_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9157580.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            46722..47348
                     /gene="rplC"
                     /locus_tag="VWX16_000139"
     CDS             46722..47348
                     /gene="rplC"
                     /locus_tag="VWX16_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9157581.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            47373..47996
                     /gene="rplD"
                     /locus_tag="VWX16_000140"
     CDS             47373..47996
                     /gene="rplD"
                     /locus_tag="VWX16_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9157582.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            47996..48292
                     /locus_tag="VWX16_000141"
     CDS             47996..48292
                     /locus_tag="VWX16_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9157583.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            48310..49143
                     /gene="rplB"
                     /locus_tag="VWX16_000142"
     CDS             48310..49143
                     /gene="rplB"
                     /locus_tag="VWX16_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9157584.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            49247..49528
                     /gene="rpsS"
                     /locus_tag="VWX16_000143"
     CDS             49247..49528
                     /gene="rpsS"
                     /locus_tag="VWX16_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9157585.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            49540..49884
                     /gene="rplV"
                     /locus_tag="VWX16_000144"
     CDS             49540..49884
                     /gene="rplV"
                     /locus_tag="VWX16_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9157586.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            49897..50550
                     /gene="rpsC"
                     /locus_tag="VWX16_000145"
     CDS             49897..50550
                     /gene="rpsC"
                     /locus_tag="VWX16_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9157587.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            50554..50967
                     /gene="rplP"
                     /locus_tag="VWX16_000146"
     CDS             50554..50967
                     /gene="rplP"
                     /locus_tag="VWX16_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9157588.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            50977..51183
                     /locus_tag="VWX16_000147"
     CDS             50977..51183
                     /locus_tag="VWX16_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9157589.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            51208..51468
                     /gene="rpsQ"
                     /locus_tag="VWX16_000148"
     CDS             51208..51468
                     /gene="rpsQ"
                     /locus_tag="VWX16_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9157590.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            51494..51862
                     /gene="rplN"
                     /locus_tag="VWX16_000149"
     CDS             51494..51862
                     /gene="rplN"
                     /locus_tag="VWX16_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9157591.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            51940..52245
                     /gene="rplX"
                     /locus_tag="VWX16_000150"
     CDS             51940..52245
                     /gene="rplX"
                     /locus_tag="VWX16_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9157592.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            52269..52811
                     /gene="rplE"
                     /locus_tag="VWX16_000151"
     CDS             52269..52811
                     /gene="rplE"
                     /locus_tag="VWX16_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9157593.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            52829..53098
                     /gene="rpsN"
                     /locus_tag="VWX16_000152"
     CDS             52829..53098
                     /gene="rpsN"
                     /locus_tag="VWX16_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9157594.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            53383..53781
                     /gene="rpsH"
                     /locus_tag="VWX16_000153"
     CDS             53383..53781
                     /gene="rpsH"
                     /locus_tag="VWX16_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9157595.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            53973..54509
                     /gene="rplF"
                     /locus_tag="VWX16_000154"
     CDS             53973..54509
                     /gene="rplF"
                     /locus_tag="VWX16_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9157596.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            54593..54949
                     /gene="rplR"
                     /locus_tag="VWX16_000155"
     CDS             54593..54949
                     /gene="rplR"
                     /locus_tag="VWX16_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9157597.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            54967..55461
                     /gene="rpsE"
                     /locus_tag="VWX16_000156"
     CDS             54967..55461
                     /gene="rpsE"
                     /locus_tag="VWX16_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9157598.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            55433..55657
                     /gene="rpmD"
                     /locus_tag="VWX16_000157"
     CDS             55433..55657
                     /gene="rpmD"
                     /locus_tag="VWX16_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9157599.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            55802..56242
                     /gene="rplO"
                     /locus_tag="VWX16_000158"
     CDS             55802..56242
                     /gene="rplO"
                     /locus_tag="VWX16_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9157600.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            56255..57565
                     /gene="secY"
                     /locus_tag="VWX16_000159"
     CDS             56255..57565
                     /gene="secY"
                     /locus_tag="VWX16_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9157601.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            57716..58354
                     /locus_tag="VWX16_000160"
     CDS             57716..58354
                     /locus_tag="VWX16_000160"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9157602.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            58471..58689
                     /gene="infA"
                     /locus_tag="VWX16_000161"
     CDS             58471..58689
                     /gene="infA"
                     /locus_tag="VWX16_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9157603.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            58714..58830
                     /gene="rpmJ"
                     /locus_tag="VWX16_000162"
     CDS             58714..58830
                     /gene="rpmJ"
                     /locus_tag="VWX16_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9157604.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            58848..59213
                     /gene="rpsM"
                     /locus_tag="VWX16_000163"
     CDS             58848..59213
                     /gene="rpsM"
                     /locus_tag="VWX16_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9157605.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            59231..59614
                     /gene="rpsK"
                     /locus_tag="VWX16_000164"
     CDS             59231..59614
                     /gene="rpsK"
                     /locus_tag="VWX16_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9157606.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59657..60592
                     /locus_tag="VWX16_000165"
     CDS             59657..60592
                     /locus_tag="VWX16_000165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9157607.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            60604..60990
                     /gene="rplQ"
                     /locus_tag="VWX16_000166"
     CDS             60604..60990
                     /gene="rplQ"
                     /locus_tag="VWX16_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9157608.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            61255..61521
                     /locus_tag="VWX16_000167"
     CDS             61255..61521
                     /locus_tag="VWX16_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9157609.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            61531..62868
                     /locus_tag="VWX16_000168"
     CDS             61531..62868
                     /locus_tag="VWX16_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9157610.1"
                     /translation="MDIRQVTETIAMIEEQSFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALPLLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            63112..63804
                     /locus_tag="VWX16_000169"
     CDS             63112..63804
                     /locus_tag="VWX16_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9157611.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLISGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(63907..65552)
                     /locus_tag="VWX16_000170"
                     /pseudo
     CDS             complement(63907..65552)
                     /locus_tag="VWX16_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(65542..66633)
                     /locus_tag="VWX16_000171"
     CDS             complement(65542..66633)
                     /locus_tag="VWX16_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157612.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLGLMQIAQYADRKPDKLSGGQQQRVTLACVLAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(66646..67767)
                     /locus_tag="VWX16_000172"
                     /pseudo
     CDS             complement(66646..67767)
                     /locus_tag="VWX16_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT        20216 a        12722 c        14905 g        19995 t
ORIGIN      
        1 atagaaagta ggttctttgt aaccgctcca ttataaaatc ctttcactct tccggtatgc
       61 tattaggaaa aacataattg gaggatattt tgagctcaca cctcatcgtt ccagtggatg
      121 ttccccaagc tcgtgtactg cccaaaaaag ttagacattc tatttagaac gaggattgag
      181 ttctgtattg tacaaggctc ggtcctttta gagtcagctt aaggctggct ttttcaatca
      241 ccaaagtgtc agaatgtttt gacaaatgaa cacaaataat gatataatat gcaaaagcta
      301 ggaggtggta ggatgattga aaaaatggaa ctgggggaat tttacaagga attacgtttg
      361 gcgagaaagc ttaagcagac agatgtggct tgtgagggtc taacagcttc tcagttgtcc
      421 aagtttgaac tagggtagtc tatgctgtct gcggataagt tgattctagc tatccaaggt
      481 ataaatgtga ccttggatga gtttgggcac aagctcaaca actatcaaga atctccacat
      541 atgcgaatcg gtcgaaaggt tgtgaatcgc tttgcccatc aggatatagc tgctttagag
      601 cagctattgg aggaagtcga tcaagaacag atggcgcaga cctatcgtcg tttgaatgcc
      661 atcgtgatta aagatgccat ccattcacta aataaaagct acccactagc agaggaggat
      721 agcgagtttt tgaccaccta tctctatgct atcgagtctt ggacctggtt tgaactctat
      781 ctcttttgca ataccatgcc cttcttgagc aatcaagatc tgattttttt atcaacctcc
      841 ttactcgaaa aatccaaaga atttaaagag ttagtacaca atcgattgta tatgaagcaa
      901 ggactcttaa atatcttatc agaactcatg gagcgaaaac ttttctccta catcccaatc
      961 ttcgaagccg agttggagag gatgttacga ccgtatgatg tttttgaaaa agtatcgtgg
     1021 caatttttaa agaagatgag tgtctttctt caaacaaagg gaagcaatca aaaagagatt
     1081 gaacgcttta tccaatctct gcaggtatta gaaaatccac aattaacatc cctttttgaa
     1141 ttgcgttttc agcaatataa agaacttata gattagttaa aaatggaatg acaataaccc
     1201 actccgaaat aagtaggagt atcagttgga tttttctttt cttcatctat tatattcctc
     1261 cttgttagta ataactttat tataccaggg aaataatcaa atctatcaaa atcgcaaata
     1321 agaaatttct ataagaaaaa atatcaaata tgcgattttt taaaataagc caattttcgt
     1381 gttatactgt acttgtaaag cacttgaagc aaatcctagg tcgcagaagt ggtttacaaa
     1441 tgaagataat tgaaggagtg taaaagatgt taaatttaca atttgcagaa acaatggaat
     1501 tgacagaagc tgagttggag acagtttatg gaggggaatt tgggaataat gctgttatcc
     1561 cagccggtgc ttggggaggt ttaggaacgt cttggttaat cactaatttc tggaagaaat
     1621 attttaacca tgattcttcc actgttaatc gtcgccatta ttgatattca gagtccttgc
     1681 ctttttcttg ggtagggact gtctttattt catagtatag gagaaaatag aatgaaaaag
     1741 ataatctcac accgctactt tattatagtt tttctactag ttattgctga ccagaagttc
     1801 agtgttttag ttttacgcag cgaccttgtt gctgggctat ctgactttgc ctattatctg
     1861 tcggatatga tgttgaattt tcttgtggtt ttatttgctc ttattgctat gatttggtcg
     1921 gggaaatggc agaaaatcaa tagtagaaag tttaaaggat cctatctttt ctattccttc
     1981 ctagctctgc ttgcttttgt tgcttggaat tttgtcacct tttttctttt cccacctact
     2041 cgaaatgaaa tttcttatca acatgctgct cctactttta caggagttac ggcatttttg
     2101 atgtattttt ttatcctgtg attgcaggtc ccatttttga agacatgatt tatcgtggat
     2161 tagtgatgac tgctctggaa aaaggaaaga aatgggggct agatgtactt ggttccgctg
     2221 ttttatttgg ggtctcgcac attagtaatc acggttgggt cttgacagac tttgtcttct
     2281 atatgggtgg aggtcttata tttgcagtct tatttagaat gacaaagtca atttattggc
     2341 ctattggact gcatatagtc tacaatggca ttggtcagct tttgatgtta ctgtaatttt
     2401 ggttgttagt agtatcgcgg tctatccttg gggaaagctt cccatttgaa tttaagaagg
     2461 agttgtgatg aaagtatttc ttcaaaatag agattttaga caattaacca tcaaccagtg
     2521 gatttcaacg cttggggata cgatttttta tctggccttc ctgaattatg tggcagatac
     2581 atcttttgcc cctttggcga ttttactcat cacgatttca gaaaccctgc cccaagttct
     2641 acaaatcttt ctgggagttt tggcggattt tcaacatcat cgtgtcttga aatacacagt
     2701 cattagtttt gcaaaatttt tgctttactc tatagtgtcc ctatcgcttt cagggcagtc
     2761 tttttccttg ttgctagtaa cctttatttg tctgcttaac ctcttgtctg acacattgag
     2821 tgctttttca ggcgccatgc tcactccgat tttcattaga attattgggc aagaccatct
     2881 ggcagaagct attggattta aacagtcaac tgttagttta gtgaaaacaa tcagtaatat
     2941 cctaggagga gtcttactag gcattctatc catccagttt atttccttac tgaacgctct
     3001 taccttttta atcgcatttt taggtatcct cttcataaaa actgacctct tgaaagtaga
     3061 aaaaacgatt agctatcaag aaggactctc tgtaaaatcc ttttgccagc atttgctcca
     3121 atcatcgaaa ttgatatgga atatgaataa ggtgctcttg gttttgttta ttatctctac
     3181 tagtcaagca gtgataaatg ttacggttcc tatttccact ctatttttaa ggaaccagcc
     3241 ctttttgaat ttacaaacag gtcaatctct tgccttgcta tccacttttg aactgtcagc
     3301 cctgattgtc ggaagccttg taagtggcta tctgaaacat acgatttcta taaaaacagc
     3361 tctctatgcc tcacttgtca tccagttgct tcttctagta ggatttgcca cagttcgttt
     3421 tgactggatt ctcatcttta gtaccttgga tgcctttttc gcaggtgttc tctctcctag
     3481 attgcaagaa ctcgttttta aacaaatacc tgaggagtca atgggagcag ttcaatcttc
     3541 tatcggtgcc attacggttg tcttacctag cttatttaca atagctttgg taaccattgc
     3601 tactagcttt ggaactctgg cagttagctt tgttttattg ctatttcttc tagttgcctt
     3661 tgttatgctt ctgaatattc gtgaaagtat ttagtggaga atttctataa tatatgtttg
     3721 agactggatt gccagtcttt ttatgatggt caaaatcaac agactttttc tcttttctga
     3781 atgtgttata gtagactgaa tctaaaatag tacgaaacaa ttgctaaaac atttatagaa
     3841 attaatttta ctttcccaat cgatttgttc tcatcttatt tcaatctgct atataatagt
     3901 ccatgctgtg actggattct tttcagccta cttatactct tcgaaactct cttcaaacca
     3961 cgtcagcttc accttgccgt aggtatggtt actgacttcg tcagttctat ccacaacctc
     4021 aaaacagtgt tttgagctga cttcatcagt tctatctgca acctcaaaac ggtgttttga
     4081 gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtatt attaagacaa
     4141 tatgaggaga aagatgaaag aaaaaggatt ttaggagggg gcgcaggcag ccatgccaac
     4201 ggctcttggc tatgtcagta tcggcctggc ctgtgggact atcggtgcgc cctatgtgac
     4261 acctgttgag atgggcttga tgagtctctt tgtttatgct gggagtgccc agtttgccat
     4321 gttggcactg attgtggttc aagctcctgt ggcagctatt gctatgacgg tttttctaat
     4381 caacttgcgt ctctttttgt tgagtttaca cgcatcgact tatttccgtc ataccagtct
     4441 ttggtacaat atcggtatgt ctagtatctt gacagatgag acctatggcg ttttgatggg
     4501 tgaattggcc catacagaca aggtaaatcc tatgtggatg cacggaaaca atcttaacag
     4561 ctatgtggct tggtttgtgg ggacagtagt cggaacggct ctgggtggcc tgctaccaaa
     4621 tccagaaatc tttggcttgg attttgccct ggttgggatg tttattggta tttttgcttc
     4681 gcaatttcag atgatgcaaa gacggattcc tgtccgcaat ctgctcatta tcctagcagt
     4741 tgttgcggtg tccttctttt tgctcttgac agtggtgtct cagtcactag ctgttctgtt
     4801 tgcgacgcta cttggttgta gcatgggggt ggttttagat ggtcagtaag tatcttttat
     4861 tagcagttat tttctctggc ttggtgactt ggattctccg tatgatttcc ttcatcttag
     4921 tcaagtataa gggcttgcct gcaatcgttg agcgtttttt gaagttcttg cccgtttcca
     4981 ttatctttgc cttgattctt tcaagcgtag tgacaggtaa ggttgggagc cttcctcaaa
     5041 ttaaatggct agacttctta gccgtctttc caacagcttg ggtagccttt cgctaccgca
     5101 atctagtcgg aacagttctc tttggagtgg tcttgattgc catcttgcgt ttggtctttt
     5161 aaattaccca taaaaaaaac ttatcacaga gatagatatc atataatggc gtaaatgctc
     5221 tttttctgtt aagattataa ggtattctat tttggaggaa atgacatgaa aaaaatcgtt
     5281 aaatactcat ctcttgctgc cctaggactt gttgctgcag gtgtgcttgc ggcttgctca
     5341 gggggtgcta agaaagaagg agaagcagct agcaagaaag aaatcatcgt tgcaaccaat
     5401 ggatcaccaa ggccatttat ctatgaagaa aatggcgaat tgactggtta cgagattgaa
     5461 gtcgttcgcg ctatctttaa agattctgac aaatatgatg tcaagtttga aaagacagaa
     5521 tggtcaggtg tctttgctgg tcttgacgct gaccgttaca atatggctgt caacaatctt
     5581 agctacacta aagaacgtgc ggagaaatac ctctatgccg caccaattgc ccaaaatcct
     5641 aatgtccttg tcgtgaagaa agatgactct agtatcaagt ctctcgatga tatcggtgga
     5701 aaatcgacgg aagtcgttca agccactaca tcagctaagc agttagaagc atacaatgct
     5761 gaacacacgg acaacccaac tatccttaac tatactaagg cagacttcca acaaatcatg
     5821 gtacgtttga gcgatggaca atttgactat aagatttttg ataaaatcgg tgttgaaaca
     5881 gtgatcaaga accaaggttt ggacaacttg aaagttatcg aacttccaag cgaccaacaa
     5941 ccgtacgttt acccacttct tgctcaaggt caagatgagt tgaaatcatt tgtagacaaa
     6001 cgcatcaaag aactttataa agatggaact cttgaaaaat tgtctaaaca attcttcgga
     6061 gacacttatc taccggcaga agctgatatt aaataatttc ttgaaatcat ccattctgag
     6121 taaggtgggt ggtttctcag tttttaggga tatagtttta aactatatat ctgactatct
     6181 aataacaatt tgtgaaatca aacaaattgt gagatactag tacggtatta tttttaagga
     6241 gaaagaatca tgaaaatcaa aaaatggctt ggcctagcag cccttgctac agtcgcaggt
     6301 ttggctcttg cagcttgcgg aaactcagaa aagaaagcag acaatgcaac aactatcaaa
     6361 atcgcaactg ttaaccgtag cggttctgaa gaaaaacgtt gggacaaaat ccaagaattg
     6421 gttaaaaaag acgggattac cttggaattt acagagttca cagactattc acaaccaaac
     6481 aaagcaactg ctgatgggga agtagatttg aacgctttcc aacactataa cttcttgaac
     6541 aactggaaca aagaaaacgg aaaagacctt gtagcgattg gagatactta catctctcca
     6601 atccgccttt actcaggttt gaatggaagt gccaacaagt acactaaagt agaagacatc
     6661 ccagcaaacg gagaaatcgc tgtaccgaat gacgctacaa acgaaagccg tgcgctttac
     6721 ttgcttcaat cagctggctt gattaaattg gatgtttctg gaactgctct tgcaacagtt
     6781 gccaacatca aagaaaatcc aaagaacttg aaaatcactg aattggacgc tagccaaaca
     6841 gctcgttcat tgtcatcagt tgacgctgcc gttgtaaaca ataccttcgt tacagaagca
     6901 aaattggact acaagaaagc acttttcaaa gaacaagctg atgaaaactc aaaacaatgg
     6961 tacaacatca ttgttgcgaa aaaagattgg gaaacatcac ctaaggctga tgctatcaag
     7021 aaagtaatcg cagcttacca cacagatgac gtgaaaaaag ttatcgaaga aacatcagac
     7081 ggtttggatc aaccagtttg gtaataagaa acagggaggt gggagagaga attccacctc
     7141 ttgcttttgt atagagcacg gagtgtaaag aagactattc gttcatagaa aggtagagag
     7201 aatatggttt ttcctagcga acaagaacag attgaaaaat ttgaaaagga tcatgtagcc
     7261 cagcattatt ttgaggtttt gcgtaccttg atttctaaga aatcagtctt tgcccagcag
     7321 gttggactca aggaagtcgc aaattatctg ggtgagattt tcaagcgtgt tggagctgaa
     7381 gtggagattg atgagagcta tacagcgccc tttgtcatgg catatttcaa gagttcgcgt
     7441 ccagatgcca agaccttgat tttctataac cactatgaca ctgtgccagc ggatggggat
     7501 caggtctgga cagaggatcc ttttacgctt tcggtccgca atggcttcat gtatgggcgt
     7561 ggggttgatg acgacaaggg tcatatcaca gctcgcttga gtgctttgag aaaatatatg
     7621 cagcgccatg atgatttacc tgtcaatatc agctttatca tggagggagc ggaggaatcg
     7681 gcttcaacag acctagataa gtatttggaa aagcatgcag acaaactccg tggggcggat
     7741 ttgttggtct gggaacaagg gaccaaaaat gccttggaac aactggaaat ttctggtggc
     7801 aataagggga ttgtgacctt tgatgccaag gtaaaaagcg ctgatgtgga tatccactcg
     7861 agttatggtg gcgttgtgga atcagctcct tggtatctcc tccaagcctt acagtctctt
     7921 cgtgctgcgg atggccgtat cttggttgaa ggcttgtacg aagaagtaca agagcccaat
     7981 gaacgagaaa tggccttgct agaaacttat ggtcaacgaa acccagagga agttagtcgg
     8041 atttatggat tggagttgcc tctcttacag gaggatcgga tggcctttct aaaacgtttc
     8101 tttttcgagc cagcgcttaa tatcgaagga atccagtctg gttatcaagg tcagggtgtt
     8161 aagactattt tacctgcaga agccagtgcc aagctagagg ttcgtctggt tccgggccta
     8221 gaaccgcatg atgttctgga aaaaattcgg aaacagctag acaaaaatgg ctttgataag
     8281 gtagaattat actatacctt gggagagatg agctatcgaa gcgatatgag cgcaccagcc
     8341 attctcaatg tgatcgagtt ggccaagaaa ttctatccac agggcgtttc agtcttgccg
     8401 acgacagcgg ggacaggacc tatgcatacg gtctttgatg ccctagaggt accaatggtt
     8461 gcattcggtc taggaaatgc caatagccga gaccacggtg gagatgaaaa tgtgcgaatc
     8521 gctgattatt acacccatat cgaattagta gaggagctga ttagaagcta tgagtagaga
     8581 tattatcaag ttagatcaga tcgatgtgac ttttcatcaa aagaagagaa ccattacagc
     8641 ggttaaggat gtgaccattc acatccaaga aggggatatc tacggaatcg ttggatattc
     8701 tggagcaggg aaatcaaccc ttgtacgggt gattaacctc ttgcaaaaac catctgcagg
     8761 gaaaattacc attgacgacg atgtgatttt tgatggtaag gtcaccttga cggcagagca
     8821 gttgcgtcgt aaacgtcaag atatcgggat gattttccag cattttaacc tgatgagcca
     8881 aaagacagca gaggagaatg tagcctttgc ccttaaacac tctggactca gcaaggaaga
     8941 aaagaaggct aaagtagcta agttgttgga cttggttggt ttggcagatc gtgctgaaaa
     9001 ctacccttca caactatctg gagggcaaaa acagcgtgtg gcaattgcgc gtgccttggc
     9061 caatgatcca aaaatcttga tttcagacga gtcaacttct gcccttgacc ctaagacaac
     9121 caagcagatt ttggccttgt tgcaagattt gaaccaaaaa ttaggcttga ctgttgtcct
     9181 gattacgcat gaaatgcaga ttgtcaaaga cattgccaac cgtgttgcag ttatgcagga
     9241 tgggcatttg attgaagaga gtagtgtgct tgaaatcttc tcagacccta aacaaccttt
     9301 gactcaagac tttatctcaa cagctacagg tattgacgaa gccatggtca aaatcgagaa
     9361 gcaagaaatc gtggaacact tgtctgaaaa cagtctcttg gtgcaactca agtacgctgg
     9421 atcttcaaca gacgagccac ttttgaatga attgtacaag cattatcaag taatggctaa
     9481 tattctctat gggaatatcg aaatcctcga tggtactcct gttggagaat tggtggtggt
     9541 cttgtcaggt gaaaaagcag cgctggcagg tgctcaagaa gccattcgtc aagcaggcgt
     9601 acagttaaaa gtattgaagg gaggacagta agatggaatc attgattcaa acctatttac
     9661 caaatgtcta taagatgggt tggtctggtc aggcaggctg gggaacagct atctacctaa
     9721 ccctctatat gacagttctt tccttcatta tcggaggctt cttggggcta gtggcaggtc
     9781 tctttctcgt cttgacagcg ccaggtggtg tcttggagaa taaagtcgta ttctggattt
     9841 tagacaaaat tacctcaatt tttcgtgcgg ttccctttat catcctcttg gcaatcttgt
     9901 caccactttc tcacttgatt gttaaaacaa gtatcgggcc aaatgcagcc cttgtcccac
     9961 tttcttttgc agtctttgcc ttctttgccc gtcaggtgca ggttgtcttg gctgaattgg
    10021 atggcggtgt cattgaggcg gctcaagcga gcggagcgac tttctgggac atcgtgggtg
    10081 tttacctatc agaaggtctt ccagatttga tccgtgtgac gactgtgacc ttgatttccc
    10141 ttgttgggga aacagctatg gccggtgcgg ttggagctgg tggtatcggt aacgtagcca
    10201 tcgcttatgg atttaaccgc tacaatcacg atgtgaccat cttggcaacc atcgttatca
    10261 ttttgattat ctttgcaatc caattcttag gagatttctt gactaagaaa ttgagccata
    10321 aataaaaaag agccgtgtgg ctctttttaa ctgatcagat tttctgggca aattttttac
    10381 tcaaggcttg tccaatcaag gcacccacta gggctccgat gacaatactt gcgataaata
    10441 gaaggacagt tccagggttt ggagcgacca tgatgcggtc gatatattct tgggattttc
    10501 ctcttgccag aagagtagcc atataggctt tgggcgcaat ccacataagc aagattggtc
    10561 ctgttgtact aaaggcgaaa ataatgaaag aaaggaagtt ctttgttttg tccttgtatt
    10621 ttcctaaatg agctactcca tctgctagga ggccacagat aattccagga aggaaggaac
    10681 cagcaccgtg tttagttccc aagaaaaaga gggcaatgac aaggccgata gtggtaatgg
    10741 ctccaaagcg cggaactttt gcgactagga tcatatagac gctaccgccg acaagggcag
    10801 taaaggcagg cgcataaaac atgtttccag tttggtcaaa gagattgccc aaaaggacac
    10861 caatccccat gcagaggaag taaagaactg caaaaagcag tgtagttaag atagatttct
    10921 tcatgaattg tctcctgata attttttcac aattctcatt ttaccatggt ttggtgggat
    10981 tgtaaatgga tactagaagt ttttgaaaac gcttgaaata tgatataatg gtttcatagt
    11041 tatttttagg ggaatatcat gggtagattt ttagactttg tctttaatcg tttcttttta
    11101 gggatgattg cgacagcctt cttttggcta ttaactttag caggagggat tatccttggt
    11161 ctagcgccgg ctagtgccac cttgatgagc ttatatgcag aacatggtta tagctttcgg
    11221 gaatacagtt tgaaggaggc ttggtctctt tacaagcaaa attttatctc aagcaacctg
    11281 attttctata gctttttagg tgtgggtcta gttttgacct atggtttgta tctcttggtg
    11341 caattgcctc atcagaccat tgttcatttg attgcgaccc ttttgaatgt cctagtagtt
    11401 gccctgatct ttttggctta tacagtatct ttaaaattac aagtttattt tgccttgtcc
    11461 tatcgaaata gtctcaaatt atccttgatt ggcatcttta tgagtctagc agctgtggct
    11521 aaggttctcc ttgggactgt gctacttgta gcaattggtt actatatgcc tgccctgcta
    11581 ttttttgtag gaattgggat gtggcatttc tttatcagtg atatgttgga acctgtctat
    11641 gaaatcatcc atgaaaaatt ggcgacaaaa tagaatgaag cacttttggc tacatacgct
    11701 tctaagaacc tatagttcag tgatgatcat tatcattgcg agttttgcaa tcttactctc
    11761 ttacgctgac tgggattcac gtgaaaagga agcccagaga gtagcccagc gtgtaactgc
    11821 tagaacagtg agtgaaattg aatattacca tagagagtca acccagatag ctcaggcttt
    11881 agttgaaaac caagctcgta ttgagggaat ctataaatac tttagcctta gcatgccaga
    11941 ctatttttac tggcaattag agcggaaagt ttcgccttat atatcagtct ctctgtatga
    12001 aaatgttgat gacctctatg ttcgaaatga ttttgtaact ggggtggcca ttgcttttca
    12061 agattacaag gaagtctatg tttctactaa agacaaacgt agtggagaaa aaatcagggc
    12121 tgaggatttc aaaccagcag gaaatagttt tgccattcca gtgtcagatc cagtgtcaga
    12181 tcaagactta ggagtgattt acatctcctt ggatcctgct gttttatacc atgccattga
    12241 taatactaga ggtcatactc cgatggcagt aacagtgacc gaaccttttg atacggagat
    12301 ttttcatatt ggtgagacag ttgataagga gagtgaaaat tggctagttg gcttaacttc
    12361 tcatggttat caggttcagg tggcagttcc caaaaacttt gttttacaag gaacggtgac
    12421 cagctctgct ttgattgtgg gcttgagcct tctctttatt gtcattcttt atctgacttt
    12481 gaggcagacc tttgctaatt atcaaaagca ggtagtggat ttggtggatt ccatccaagc
    12541 tattgcccaa ggacaagaag gtcttcgcat tgatacgctt gaaaaggatc aggaattgct
    12601 cctaatcgcg gagacgacca atgatatgtt ggatcgattg gaaaagaata tccatgatat
    12661 ttaccagtta gaactcagtc aaaaagatgc caatatgcgg gccttgcagg cgcaaatcaa
    12721 tcctcatttt atgtataata cgctggagtt cttgcgcatg tatgcagtta tgcagagtca
    12781 agatgagttg gcagatatca tttatgaatt cagtagtctc ttgcgtaaca atatttccga
    12841 cgaaagagag accctcctca aacaggaatt agaattttgc cgtaaataca gctatctctg
    12901 catggttcgc tatcccaagt ccattgccta tggtttcaag atagatccag agttagagaa
    12961 tatgaagatt cccaagttta ccttgcaacc gctggtagaa aactatttcg cgcatggtgt
    13021 tgaccacagg cggacagata atgtgattag catcaaggct cttaaacagg atggttttgt
    13081 ggaaattttg gtggtcgata atggtagagg aatgtcggct gaaaagttgg caaatatccg
    13141 agaaaaatta agtcagagat attttgaaca ccaagccagc tacagtgatc aaaggcagtc
    13201 tatcgggatt gtcaatgtac acgagcgttt tgtgctctat tttggagacc gctatgccat
    13261 tactatagag tctgcagagc aagccggtgt tcagtatcgt attacaattc aagatgagta
    13321 gaaagggaga aaatgtataa agtattatta gtagatgatg agtacatggt gacagaaggt
    13381 ctgaagcgtt tgattccctt tgataagtgg gatatggagg tcgtcgcaac agtcagtcat
    13441 gccgatgaag ctctagaata tgttcaggaa aatcctgtcg atgtcatcat ttccgatgtc
    13501 aatatgccag acaaaacagg gcttgatatg attcgggaga tgaaagagat cttaccagat
    13561 gctgcctata tcctgctctc aggttatcag gagtttgatt atgtaaaaag agcaatgaat
    13621 cttagtgtgg tggactattt ggtcaaacct gttgataagg tagagctggg aaatctgctg
    13681 gagaagattg caggtcagct cggcgagaga gggaagaaaa gtcagactct tagtcaagaa
    13741 ttagacgagg ctggatttgt tagttattta ggggataagg agaattggtg gataggtcta
    13801 tccaaggaaa aacaaggttc cttcaccatt ccctactatg tcttgggtca agcctggcag
    13861 attttcattt ctgaccaacc cctagatggt ttagtcgtta caccttttga agctccttat
    13921 caagaacatt ttgaacgctg gaagctgaat gctgagaaaa ccctctttta cggttctgta
    13981 aatctgcagc agtctgagag tctctttgcc tattacgaac cgatttatag ggttatcatt
    14041 cagggaaatc tcaatcaaat cgtagaagag ttaaatctct tggagaaggt agttcttgaa
    14101 aatacgccgc gaattccgat tactaaacag ctttttatcc agtttgtcat ggatgtcttc
    14161 catttatttg aacatctcaa agctgatgat atgacggaca ttgtcaaaac cattcatgct
    14221 attcaatcct tcgatgaatt ggtttcttat atcaaggaaa ctctgatcag ctttttcggt
    14281 caataccgta tgaatgaaaa tgtggtcagt gtgctggaag tcattggtcg tgattaccaa
    14341 aaagagcttt ccctcaagga tatcagtaag gccctcttta tcaatcctgt ctatctaggg
    14401 cagttgatta agcgtgaaac cgattcgacc tttgcagagt tactaaacaa acaacgtatt
    14461 aaggctgccc aacaactttt gctttcaact agtgacagca tcgaagatat ttgttatgct
    14521 gttggttaca gtaaccttgg atatttctat aaagttttcc gaaaattgtg cggaaaatcg
    14581 ccaaaagcct accgaaaaca ggtagaaact atactataag atttgtattc ctttacaaaa
    14641 tgtgctataa tattattagg aggttctatc atgaaaaaga aaccaattta tctatgggtc
    14701 ttgctaatct tgtctgccct tatttcagtt ccgtctctgt ttggaattgt gagtcccttg
    14761 cctagtaaag aagcccttcg tgctgctcag aaacaagttg caggggtcaa tgctcagcaa
    14821 ttagaagacc agcttaatta tacctataga gtagcagaag catctcattc tatttttaat
    14881 gttgctttga ttgtgctatc tactatttta gtggtcgtag cgattgtctt ccttgttcgt
    14941 aaaaatttgc aatatgcaaa ctatacttac gttggctatg ttttgttagc tattattggt
    15001 tcgatttatg gctatgttgg tttacaagac gctgtacagt tggttcaaga tgagagcatg
    15061 cgtttgacag tgagtattgg ttcaaaagct gttagcattt tctatatcgt tatcaatgtt
    15121 ctcttcctag cccttgtctt ctataagatg tggcgacaac agaaagcctt ggcagaagaa
    15181 gaggaaacag aagagcttgc ctaagtattg acaaaaaaga actatcaaga tacaatcttg
    15241 gtagttgttt tttgattata gataaaatca tggaggtaca tatgaagaca atttctttag
    15301 tttatattag tttgagtggc aacactgaaa gttttgtgac gcgcttgaaa gactatctct
    15361 tgtcccagta caaagggatt gaggttcaaa agattcatat caaggatttg gtcaaggaag
    15421 gcaaaaattt ctatgaaata gaccatccct atgtcgcttt tttgccgacc tatctcgaag
    15481 gtgggaatgg cgtggatagc ggagatgttg agattttgac gacaccggtg ggagatttta
    15541 ttgcctatgg taacaatgct agtaagtgtt ttggtgtggt tgggtcagga aatcgtaact
    15601 ttaataacca atactgccta acagccaagc aatacagtca acgctttggc tttcctgtat
    15661 tggctgactt tgaaatgcga ggcatgctgg aagatatcaa acatgtcgca gctattatcg
    15721 cagatttgta tgagttggaa aaggagaatt aatactcaat gaaaatcaaa gagcaaacta
    15781 ggaagctagt cgcaggttgc tcaaagcaca gctttgaggt tgcagataga gctgacgtgg
    15841 tttgaagaga ttctcgaata gtataaagtt cttataagag gtggttgaag ttgttcaact
    15901 gttttttgag tataaacagt ctttaagaac gtaaaaaagt ataagttcag gtcttcaaaa
    15961 ccttgttctt atgcttttta tcatagaaat atttgctgaa ttttctcttg aaatgaaacc
    16021 ttatcttcat ctcgcgcttg tcccttaccc tgcttttccg attgcgagtg cgtagataaa
    16081 gaagatggcg aagccaaaga ggaaaatcaa tcccgcaatg gcaaggtgtt tgagccaaga
    16141 aggaagattt ttgttttcac gtgttaccaa ggcgtaattc aaaagagcaa agaaaggtgt
    16201 tgtcaggaaa gagccaatca tggcaaagcg gagcatggtt gaaacctgac cagcgaagaa
    16261 cttgataatg atgataccga tgatagcagt gatggtcatc cagatgttca aagatttacg
    16321 attgtcctct ttttgactga ttagcagtcg gagagattcc tgattaacgc gagaatagcc
    16381 atcgataaca gttataactg ttccaaagat acagaggaag gcaataaagg taatcaagta
    16441 acgggaccat tcgccaagaa cagaggcata catgcccacg aattgagaga tgtatttggc
    16501 tgaagcagct tcaaccgcct gccctgtagg atactgaatc agtgctccca gtgccacaaa
    16561 gaagacggct aggatagctg ttccaatata accagtgtta aagtcaaaca gagcgtcttc
    16621 tgtgttaaag ttgacggtct ttctcttttc agctgaccaa agtgaattga tggctgaaat
    16681 ttcaatagga gccggcatcc atcctaggag ggagacgatg aagggcagag ctgccatttg
    16741 ccaaggtgtc ttctcgacaa aatcagaact gtattctgga tgcttgaccg ccgcaatgat
    16801 aactgcaaga acagtcgcaa tggttaaggc agacataatc catttgacca tgccgtctaa
    16861 aagtttgtag cctccaaaga gtagcatagc ccaaatgatt gcaacgagaa tgagggacca
    16921 ctgagtaatg ctaagtccaa tcattgggaa ggcactggcg atgatagctg agcacagaat
    16981 ggcaacacca gccgtgttga ccatagtcga aaagacattg aggataaaga aaatccagag
    17041 atagagtttt cctttttcgg cataaccttc aaccaaagtc tttccagtat cagctgtgta
    17101 ttcagcacca aaacggaaaa atggatattt aaagacattg gctaagatga ccaagagaag
    17161 tagagaccaa ccgtaagaac cgccagcttg agttgaggat acaatgtggg aacctccaac
    17221 agcggcagtt gccattagga ttccaggtcc cattgctttt agcttgcttg cccaggttga
    17281 ttgatttaag gaaatagctt gcgacatgat aaatactcct ttttgaattt tcagaataat
    17341 ctgtatatgt aatctatatt ctacaaaaaa ttttggaaaa atgcaagaat attttgaaaa
    17401 aagatagaaa atttacaaag aaaatctgaa ataactgtta tgatatagcg gattgaaact
    17461 agaatagtac acctttgctt ctaaaacatt gttagaaatc gatttgactg tcctgatcga
    17521 tttatcctgt tcttatttca tttcactata gtaaggtgca gcaggataaa gaaaaaccga
    17581 gaagtttctt tctcggattt gcttattatg aggttcctat tttctatgct ccaagagttg
    17641 attgatgtga tctacttttt tagcctctct tttatagaga atagcccaga tgatgagata
    17701 gacaatcgca aactcgataa tgagctggag gtagaaaatc cagtggaaag ggaaccaacc
    17761 agcaagagtt gctagtggga caaagccagc cagcataagg aagaaatgag tcagagtggc
    17821 acgaagcatg ctccagtcac ggctgaataa gcggttgcca aagttgaaga gcataccgat
    17881 ggctgcccag ataagtgtgc agtagagcaa gaccagggca ccgtgaacct gatgctgatc
    17941 catcacttgg ccgatgagag agtagggatt tagtggtgcg taggtatttg gtgcataaat
    18001 gagtgaaaag aggatggaga ggatgaggcc gataagaaca ccggcagctg catcgtgaaa
    18061 tacttgtttt ttcatagttc taatttctcc ttgatggttt ttagataacg gcgtgaagag
    18121 taggtgaagc tttcgttttt caagaaaatt tctaccagac cgtttggcgt gagcttgagg
    18181 tgagagatgg aatcgatatt gatgatttct gattgggaaa tttggataaa attgcttggc
    18241 aagagtttaa gaacctgata gagtcgcaaa tcaatgctgt aggtctgact cgcggtttct
    18301 gctagaacct tccgattctc gatatagaag cgctgaatct tgccaatctc aactagatag
    18361 acctgatcat cgattttccc tttgattttt tctctttggt ccagattttc tgcgaactcg
    18421 atgactttct ggactttttc ggtttcttga ggtgcttgga caatcagctt ttcctcctcg
    18481 taagtctcac taatctgtag ttctactttc ataaatttct ctccttgttt ttcatacaaa
    18541 attaatctca cttcttgtac acctttatta atactcttca aaaatctctt caaaccacgt
    18601 cagcgtcgtc ttgccgtagg atagttgttg actttgtcag ttttatctac aacctcaaaa
    18661 cagtgttttg agctttctgc gcctagcttt ctagtttgtt ctttgatttt tattgagtat
    18721 accactattt tacgcccttt ggcaaggtac tttgtctatg tggagggatt gggctcctat
    18781 gtggtggagc ttttctgttc tttctgaaat atggtataat agcactaatt aatttctagg
    18841 aaaatagata cagaaagggg ctgaaagatg tctcatatta ttgaattgcc agagatgctg
    18901 gcaaaccaaa tcgcggctgg agaggtcatt gaacgtcctg ccagtgtggt caaagagttg
    18961 gtagaaaatg ccattgacgc gggctctagt cagattatca ttgagattga ggaagctggt
    19021 ctcaagaagg ttcaaatcac ggataacggt catggaattg cccacgatga ggtggagttg
    19081 gccctgcgtc gccatgcgac cagtaagata aaaaatcaag cagatctctt tcggattcgg
    19141 acgcttggtt ttcgtggtga agccttgcct tctattgcgt ctgttagtgt cttgactctg
    19201 ttaacggcgg tggatggtgc tagtcatgga accaagttag tcgcgcgcgg gggtgaagtt
    19261 gaggaagtca tcccagcgac tagtcctgtg ggaaccaagg tttgtgtgga ggatctcttt
    19321 ttcaacacgc ctgcccgtct caagtatatg aagagccagc aagcggagtt gtctcatatc
    19381 attgatattg tcaaccgtct gggcttggcc catcctgaga tttcttttag cttgattagt
    19441 gatggcaagg aaatgacgcg gacagcaggg actggtcaat tgcgccaagc aatcgcaggg
    19501 atttacggtt tggtcagtgc caagaagatg attgaaattg agaactctga cctagatttc
    19561 gaaatttcag gttttgtgtc cttacctgag ttgactcggg ctaaccgcaa ttatatcagc
    19621 ctcttcatca atggccgtta tatcaagaac ttcctgctca atcgtgctat tttggatggt
    19681 tttggaagca agcttatggt tggacgtttt ccactggctg tcattcacat ccatatcgac
    19741 ccttatctag cggatgtcaa tgtgcatcca actaagcaag aggtgcggat ttccaaggaa
    19801 aaagaactga tgactctggt ttcagaagct attgcaaata gtctcaagga acaaaccttg
    19861 attccagatg ccttggaaaa tcttgccaaa tcgaccgtgc gcaatcgtga gaaggtggag
    19921 caaactattc tcccactcaa agaaaatacg ctctactatg agaaaactga gccgtcaaga
    19981 cctagtcaaa ctgaagtagc tgattatcag gtagaattga ctgatgaagg gcaggatttg
    20041 accctgtttg ccaaggaaac cttggaccga ttgaccaagc cagcaaaact gcattttgca
    20101 gagagaaagc ctgctaacta cgaccagcta gaccatccag agttagatct tgctagcatc
    20161 gataaggctt atgacaaact ggagcgagaa gaagcatcca gcttcccaga gttggagttt
    20221 ttcggacaaa tgcacgggac ttatctcttt gcccaagggc gagatggact ttacatcata
    20281 gatcagcacg ctgctcagga acgggtcaag tacgaggagt accgtgaaag cattggcaat
    20341 gttgaccaga gccagcagca actcctagtg ccctatatct ttgaatttcc tgcggatgat
    20401 gccctgcgtc tcaaggaaag aatgcctctc ttagaggaag tgggcgtctt tctagcagag
    20461 tacggagaaa atcaatttat tctacgtgaa catcctattt ggatggcaga agaagagatt
    20521 gaatcaggca tctatgagat gtgcgacatg ctccttttga ccaaggaagt ttctatcaag
    20581 aaataccgag cagagctggc tatcatgatg tcttgcaagc gatctatcaa ggccaatcat
    20641 cgtattgatg atcattcagc tagacaactc ctctatcagc tttctcaatg tgacaatccc
    20701 tataactgtc ctcacggacg tcctgttttg gtgcatttta ccaagtcgga tatggaaaag
    20761 atgttccgac gtattcagga aaatcacacc agtctccgtg agttggggaa atattaaaag
    20821 tataaaaaag tctgggaaaa attttcaaaa tcagaaaaac gcataaaatc aggtgttcaa
    20881 aaaccttgat tttatgcgtt ttatcataga aatatttact tcatgttttc ctaattcttt
    20941 tcgaaactct ttttaaacca cgtcagtttt atcagtaatc tcaaaacagt gttttgagct
    21001 aattttgcca gttttgtctg taacatcgaa gttgtgtttg accactctgc gactggtttc
    21061 ctagtttgct ctatgatttt cacagagtat taaattgcga ttttgccaag tttctttatt
    21121 cgtctaaaag tagagtctgt tctatgcgtc taatgtacga attaggttga ccatttcaat
    21181 agctccttgt gcacactcag aacccttatt tcctgcttta gtaccagctc gttctatggc
    21241 ttgttcaatt gtatctgtcg ttagcacacc aaacataaca ggaatttcgc tatttaaact
    21301 gatttgggcg attcctttag atacctcgct acatacataa tcataatgac ttgtattccc
    21361 tctaatgaca gctcccaagc agataattgc atcatatttt ttactttttg ccatttttga
    21421 tgcaatcagt ggtatttcaa aagctcctgg aacccaggct acctcgatat ctttctcgtt
    21481 tacattctct cttttgagat tatctagtgc tccagataat aattttgaag ttataaattc
    21541 attaaatctc gctacaacaa tacctatttt aatattgttt gctactaaat taccttcata
    21601 agtgttcatt tatttttcct ccatatttaa aatgtgaccc attcgatttt tctttgtttc
    21661 taaataaaaa ctatcgtaag gattggcttc tatttcgatt gatattctac tggaaatggt
    21721 aattccatat ttttctaact gttcaacctt gtcaggatta tttgtcagta aatgaagtga
    21781 ctgaagtccc agatctttaa gcatttgtgc tccaatatga tattctctta aatcaccttc
    21841 aaagcctaat gcaagattgg catcaagcgt atccatgcct tgatcttgta aatgataggc
    21901 ttttaattta ttgataagtc caattcctcg tccctcctgt cgcaagtaaa gtaagacacc
    21961 cgaaccattc tcaacaatca ttttcatagc tttatcgaat tgctgtccac aatcgcaacg
    22021 taaagagcct aaaacatctc ctgttaaaca ttcggagtgg acccgacata atacattggc
    22081 ttcatcctct atgtttccca taataagagc aagatgatgt tccccattta gtttatctat
    22141 atagctaatt gctttgaaat taccgtatct agtaggcata ttgacagttg aaactcgttc
    22201 taccagctga tcatatactt ttctatattc ttgtaattct ttgatggtaa ttagtggaat
    22261 gttgtgtttc ttcgagaact gaattaaatc atctgttctc atcattttgc catcatgatt
    22321 cattatttca caacataggc cacactcttt tagtccagct aattttaata aatcaacagt
    22381 tgcttctgtg tgtccatttc tttctagaac accacctttt tttgcaatta aaggaaacat
    22441 gtgtcctggc ctgcgaaaat cagagggtgt tatatcttca gctacacaca tacgtgcggt
    22501 cagtcctctt tcctcggcag aaatacctgt ggtcgtttct ttataatcaa ttgaaactgt
    22561 aaaagcagtc ttatgattat ctgtattgtt ttcaaccata ggtgaaagca ttaattgatt
    22621 agctaaactt tcgctcatag gcatacaaat taatcctttg gcataagtag ccataaaatt
    22681 aacattttct gttgtagctg cttgtgcaga acaaattaag tctccttcat tttctctatc
    22741 cttgtcgtct ataacaagaa caagtcgtcc cttctgcaat gcttctaatg cttcttgtat
    22801 ttttcgatat tccattgact gattatcctt tctgctaaaa tccattttga tataatagtt
    22861 ccttcgatat ttctgatttt ggagagttat ccatcagttt ttgcacatat ttacctaaga
    22921 tatcattttc aagatttact gtactcccga cttgtttact cttaagaatg gtttgtttca
    22981 aggtatgagg gataacagat actgaaaagt ttactttgga gactttagcg acagtcagac
    23041 taatgccgtc aattgtaata gatccttttt caactattaa atctaaaatt tctttttgtg
    23101 tgttgatttg ataccataca gcattatcat ctttttttat tgacgagatt tttcctgtac
    23161 catcaatgtg tcctgtaacg acgtgacccc caagtcgacc gttgacagat aaggctcttt
    23221 ctagattcac ctcacttcca tgttttaata gagtaagagc tgttcgactc catgtttcat
    23281 tcattacatc aactgtaaag gattgatgat tgaaatgagt aactgtaaga cagataccat
    23341 ttactgctat actatcgcct aaatggatat ccgttaatat ttttgaggct ttaattgata
    23401 gtttacaatt acgagagtct ttctgtattc tttcaacttt tccgatttct tcaattattc
    23461 ctgtgaacat ggataaatca cttcactttc tatgagatag tcatttccta tttgagaaaa
    23521 agcataaggt ttcaatctaa tagcgtcatt tgacaaagaa atgccttcac ctccgacagg
    23581 aaacttggca ctgcctccaa aaatttttgg tgcaatatat attttcagct catcaacaat
    23641 ttgttgttcc aaagcactcc aattcattag actgcccccc tctagaacta ggctatcaat
    23701 ctgcatgttt cctagatgtt gcattaaact cgataagtct atatgattgc cttttttctt
    23761 tatggaaagt atttcacagc catgattttg atatagcttc attttatttt tgtcttcaga
    23821 ggaagtggca atgtaagttt taatatcatt tgctgttttt acgattttag aggtaagagg
    23881 agttcgtaaa tgtgtatcgc atatgatacg gataggattt ttcccttcct ccaatctaca
    23941 tgtcagcaaa ggatcgtctt gaataacagt attgactccc accataattg cactaacata
    24001 gtgtcgtaac tgatgcacat gctttcttgc ttcttcttca gtaatccatt tggattgatt
    24061 tgttttagtg gctatttttc catccattga cattgcatat ttcataaaaa catagggtac
    24121 atgctgggta atatactttc taaaactttt tattaagtta agacactcat tttctaaaat
    24181 tccaacagta acttgaagat tattttcctc aagtatcttt actccttttc cagatacaat
    24241 aggattacag tctaggcttc caatgactac tcttgtaata ccactatcga ttatagcatc
    24301 tatacaggga ggtgttttcc cgaagtgaca acagggttca agtgttacat aaagcgtcgc
    24361 tccgacaggg gattttctac agtttttaag agcatttctc tcagcatgtg ggccaccaaa
    24421 aaactcatga taaccttgtc cgataatgtg attatctttt acaataattg cgccaaccat
    24481 aggattggga ttgacgtaac cagccccttt ttgtgccagt tttattgcta atttcatata
    24541 ttttgaatcg ctcatctcac tacctccaaa aaaatatacc ttgaataggg gactactcaa
    24601 ggcatacaaa agaaaactta tgcgattaac aaaaatgctc tgaaatgaca agtaatcatt
    24661 tcagagcacg caaaaagcac aaatatactt ttatcttctt tcatccagac tatactgtcg
    24721 gctttggaat ttcaccaaat catgcctttc ggctcgtggg ctataccacc ggtagggaat
    24781 tgcaccctgc cctgaagata gttattcaat tacagatgat tatagtactt aattttgaat
    24841 atgtcaacag ataaatacag attgtttttg atatactgta tttgtgataa tcgattctcg
    24901 ctcctcggat aaagaaaata tgatatacta gataaacgaa ataagagaga aggaacacta
    24961 tgtacgcata tttaaaagga atcattacca aaattactgc caaatacatt gttcttgaaa
    25021 ccaatggtat tggttatatc ctgcatgtgg ccaatcctta tgcctattca ggtcaggtta
    25081 atcaggagga tcagatttat gtgcatcagg ttgtgcgtga ggacgcccat ttgctttatg
    25141 gatttcgctc agaggatgag aaaaagctct ttcttagtct gatttcggtc tctgggattg
    25201 gtcctgtatc agctcttgct attatcgctg ctgatgacaa tgctggcttg gttcaagcca
    25261 ttgaaaccaa gaacatcacc tacttgacca agttccctaa aattggcaag aaaacagccc
    25321 agcagatggt gctggacttg gaaggcaagg tagtagttgc aggagatgac cttcctgcca
    25381 aggtcgcagt gcaagcaagt gctgaaaacc aagaattgga agaagctatg gaagccatgt
    25441 tggctctggg ctacaaggca acagagctca agaaaatcaa gaaattcttt gaaggaacga
    25501 cagatacagc tgagaactat atcaagtcgg cccttaaaat gttggtcaaa taggagcaga
    25561 gaatgacaaa acgttgttcg tgggtcaaga tgaccaaccc gctctacatc gcctatcatg
    25621 atgaggagtg gggtcagccc ctccatgatg accaagcatt gtttgagttg ttgtgtatgg
    25681 aaacctatca ggcaggcctg tcttgggaaa cggtactcaa caaacgccaa gctttccgag
    25741 aagcctttca tagctatcaa attcactcag tcgcagagat gactgacact gaattggaag
    25801 ccatgctgga gaatccagct atcattcgaa atagagccaa gatttttgct acacgcgcta
    25861 acgcccaagc ctttctacag ttacaggcag agtacggctc ttttgatgcc tatctttggt
    25921 cttttgttga ggggaaaact gtcgttaacg atgttcctga ttatcgccaa gccccagcta
    25981 aaacaccctt atctgagaaa ttagccaaag atctcaaaaa acgaggcttc aagttcacag
    26041 gcccagtcgc cgtattgtct tttctacagg ctgcagggct agttgatgac cacgagaatg
    26101 attgtgagtg gaaaggtctt aaatgatgtc taacaaaaat aagggaattc tgatttttgt
    26161 gattctctat acagtcctct ttgtgtttga tggcgttaaa ttgctggctt ctttaatgcc
    26221 atttgccatt gcaaattatc ttgtttatgt agttttagct ttatatggct ccttcttgtt
    26281 caaggataga ttgatccaac aatggaagga gattagaaag actaaaagaa aattcttctt
    26341 tggagtctta acaggatggc tctttctcat tctgatgact gttgtctttg aatttgtatc
    26401 agagatgttg aagcagtttg tgggactaga tggacaaggt ctaaatcagt ctaatattca
    26461 aagtaccttt caagaacaac cactactgat agctgttttt gcttgtgtca ttggacctct
    26521 ggtagaagaa ttatttttcc gtcaggtctt attgcattac ttgcaggaac ggttgtcagg
    26581 tttactaagc attattctgg taggacttgt ttttgctctg actcatatgc acagtttggc
    26641 tctatcagag tggattggtg cagttggtta cttaggtgga ggccttgcct tttctattat
    26701 ttatgtgaaa gaaaaagaga atatctacta tcccctactt gttcacatgt taagcaacag
    26761 cctctcctta atcattttag ctatcagtat agtaaaatga aataagaaca ggacaaatcg
    26821 atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta ctattctagt
    26881 ttcaattggc tgtaatatgt gatgaaaatg ccagtaataa taccgagaaa aaagctgaga
    26941 aacttttccc agctttattt gttatagtca aagcgaatga cttgttcctg tgcatctaca
    27001 tgagcatgaa ccccaaaggg tacaattgct cttggagttg cgtggccgac attcagatta
    27061 tagacaatcg ggatattgct gtcaatgata tccaatagtg cctctttata gtcgtcatgg
    27121 aaagtttcat ccataggttt tccgaccaag agtccattga tgaccgcgaa tatgccagtg
    27181 tcctttaaag ttagcaacat ctttttgaag tcttctggtt taggcttttc ttcgcttgtt
    27241 tcgagcaaga ggattttccc ttcccagtct gacaagtcag ggaaaagttt gtatttttgg
    27301 cagagttccg tgctatctgc gtatcgagag ttgtcaaaga tatcgtagag ggattcgagg
    27361 caaccaccga ggattttccc ctcgaactga gcacttcctt gcaacaagtc aaaacctgta
    27421 tttgtatgac tgacacgagg tgttcccagg gctgtgggac taaaatcagt tcgttcctca
    27481 taccaaacgt cactagggcg gatttctgaa attcttcccg tcttaatcaa ttctttaaag
    27541 tagtgaaggc tataggctag catttctttg tctaattcac aaatgtctgc taaaaaggat
    27601 tgaccataaa aagtcttgat tcctaattta tgcaacatga ggtggttcat ggttgtatcc
    27661 gagaagccaa gaaaaatttt ttgcttgata accttttgga gttggtcatt ttcaaaaaga
    27721 taaggtagca agcgataggt atcgtctcca ccgatggcac ataggatcat gtcgatgcta
    27781 tcatcagaaa aggcatgaat caaatcctct gcacgagctt caggatggtc cttgataaag
    27841 tctaatcctt ttaacgaatg ggacaaaaag atgggattga gtcccagatg cttgagacgt
    27901 tggataccca agtccacttc gtgtttgaca aaatcctctc cgataatgcc actagataaa
    27961 ctaacaatac caatagtaga aaccatatct catcctccta gaaatagatt gagcctattt
    28021 tatcacaaaa tataagagaa agctatgtgg gatttcagaa gaatactttt aatactcttc
    28081 gaaaatctct tcaaaccacg tcagttttat ttgcaacctc aaagctgtgc tttgagcaac
    28141 ctgcagctag cttcctagtt tgctctttga ttttcattga gtataactca aaaaagtaag
    28201 tgaaaaagca accgcacctg cagttgcttt tatcgttttc ttattcttga tagcgtgttg
    28261 gtcctgcact tgcactacgg atattcaagc gtttcttgag atagaagcga agggctagga
    28321 gggctgctcc aataatagct agtggcaatg gatccagtac tgggttaagg ctagctggta
    28381 ggaagcttgt tgcaaagaag acaagcaacc aaaggaacat agaagctagg ataactagta
    28441 cagatttcca gaaaggtgga cgttgactgc ggtccatatc tggtccatag tattggtaaa
    28501 caaagtagta catcaagtag aaggcaaatc caccaaccag tccaactaat agaagagtaa
    28561 tcaatccata gccgaaagct tgatctgccg caaagaaggt tgtgagggcg ctgacaaggg
    28621 caaagaggct agtgatgaaa agagctgagt ccataatcat cagttttgga tcatcatttt
    28681 cttttggatg ctctttttcg tactgctctt tgacagtgaa gctatgagcc caatgagttg
    28741 gtgcgccgta tagggaacgg gcagttgtac ctttagattg ctcctcaagg atttggggaa
    28801 tgacttcctc aaaaatagtc tggatttcag cgtctgtttt cccatctttg atgaattgtt
    28861 gggtagcaat gtggacaaac tcttggtttt tcttagttaa tttttgtaga tcaatttgag
    28921 acataggaac tcctcttaga accatttttt atggatgaga tagagagtga gcgagacact
    28981 catagcaaag gcgataaaga cgattaacca gaaggcattt ggctctccgt ttagggggat
    29041 ttcattatcc ttaaagttca tcccgtaggc agaaaagacc atggttggga tggacatgac
    29101 gatggtcaca agggccaagg ttttcatgat gttgttctgg ttgttagaaa tgatagaggc
    29161 aaaggtctct gtcatagaat gcaagacgtt tccataaata tctgccatct cgatggcctg
    29221 ttgggtttca atcagggtgt cttcaagcag gtcttcgtcc tcaaggtatt tcttgatatt
    29281 gctggttgaa ctggtcaatt tcttaatcac gcgctcattt gttttgaggg aggccttgaa
    29341 atagacgata gttttttcca attccatgag ctcaatcaat tcttcattac gagttgattg
    29401 atgcagttga ctttcgattt gttcactctt gcggtcgatt gaacgaaggg ctgttaggta
    29461 aagctctgca ttgcgataaa gaatttgaaa gataaaacgt gaacgcatga aggtatagaa
    29521 attacgcaat cgacggttga taaagacatc aaggacaggt agtggttcca aacacgtagt
    29581 gataatggtt tcctcagtga tgataatacc aagcgggatg gttacgtagt aggtgcggtt
    29641 atttctttcc tccgtgaccg gcacgtctac gataatcagg gtatactcgt cttcaatggt
    29701 aatacgagac atttcttccg catcgagcgg tgctcgaagg tcggcaatat caatatcgaa
    29761 ggtgttggcg atttcgagtg attcattttg agtcggattg acgagattga tccaagtacc
    29821 cggttcaagc gtatcgatct ctttaaattc agttgttgta gagagaaaaa cttgtttcat
    29881 atcccttatc ctttctcatt tttcagattt tttcacactg tactattata ctacaaaatc
    29941 gaccttttgg gtaatagaat ctcacttttt ttggtataat ggtaagcaat aatggactag
    30001 aaagaacaaa gatgcaagat aaaattgtca ttcatggggc gcgtgcccat aatttaaaaa
    30061 atattgatgt ggagattccg cgagacaagt tggttgtcgt gactggcttg tcaggttcag
    30121 ggaagtccag tctggctttt gatactctct atgcggaggg acaacgtcgc tatgtagaga
    30181 gtttgtccgc ctacgctcgt cagttcttgg gaaatatgga aaaaccagat gtagatgcca
    30241 ttgatggtct cagcccagct atttccatcg accagaaaac gactagtaaa aatcctcgct
    30301 cgacggtggg aacgacgact gaaatcaatg actatctgcg tctcctctac gcacgtgtgg
    30361 ggacgcctta ctgtatcaac ggacatggag ctatcaaggc ttcttctgtg gagcaaatcg
    30421 ttgataaggt tttggagtta cctgaacgcc agcgcttgca gatcttggct cctgtcatcc
    30481 gcaagaaaaa aggccaacat aagagtgtta tcgagaaggt tcagaaagac gggtatgttc
    30541 gtgttcgtgt ggatggggaa gtctatgatg tgaccgaagt gccagagttg tctaagagca
    30601 agcaacacaa tatcgatgtc gtggttgacc gtattgttat caaggagggc attcgtagtc
    30661 gtctctttga ttccattgag gctgcccttc gtattgcaga aggttatgtc attatcgaca
    30721 ccatggacga ttcggagttg ctcttctctg agcattatgc ctgtccagtt tgtggtttta
    30781 ctgtcccaga gttagagcct cgtctcttct ctttcaatgc tccttttggc tcttgtagtg
    30841 agtgtgacgg cttgggcatc aagctggagg tggatactga tttggtagtg ccagatgcca
    30901 gcaaaacctt acgtgaggga gcgctagcac cttggaatcc tatctcatcc aactactatc
    30961 caaacatgtt agagcaggcc atgaaagtct ttggagtggc tatggataag ccttttgagg
    31021 acctgtcaga agaagataag aacttgattc tctatgggtc agatggtaag gaattccatt
    31081 tccactatga gaatgaattt ggtggcgtgc gcgatatcga cattccgttt gagggagtta
    31141 tcaataatat caaacgtcgt taccatgaaa ccaatagcga ttacactcgc actcagatgc
    31201 gtctctacat gaatgagctg acctgcggaa cctgtcaagg ctatcgtctc aatgaccagg
    31261 ccttgtctgt ccgtgtgggc ggccagcaag ggccacatat tggagaaatc tcagacctgt
    31321 ctatcgctga ccacttggac ttggtgagcc agttaacttt gtctgaaaat gaagccatca
    31381 ttgctcgtcc cattctcaag gaaatcaagg atcgtttgac cttccttaat aatgtgggtc
    31441 ttaactatct aaccctgtca cgttcagcag gaaccctttc aggtggggaa agtcagcgca
    31501 ttcgtttggc aacccagatt ggttccaacc tatcaggcgt cctctatatc ctagacgagc
    31561 cgtcaatcgg tcttcaccag agggacaatg accgcctgat tgccagtctg aaaaagatgc
    31621 gtgacttggg caatactctt atcgtggtgg aacacgacga agataccatg cgtgaggctg
    31681 attatctgat tgacgttggt cctggtgccg gtgtttttgg tggtgagatt gttgcagcag
    31741 gtacacctaa gcaagtggct cgcaacagca agtctatcac aggtcagtac ttgtcaggca
    31801 aacgtgtcat cccagtacca gaagagcgcc gtgtcggaaa tggtcgtttt attgaagtga
    31861 taggagcgcg tgagaacaac ttgcaaaatg tcacggctcg ctttccacta ggaaaattta
    31921 tcgcagtgac aggtgtgtcg ggttcaggga aatcgactct aatcaacagc attctcaaaa
    31981 aagccattgc ccagaagctc aaccgtaatt cagacaaacc tggtaagttt aagactatta
    32041 cagggattga gcatgtcgac cgtttgattg atattgacca gagccctatc ggacggacgc
    32101 cgaggtctaa cccagctacc tatacaggag tttttgacga tatacgtgac ctttttgctc
    32161 agacaaatga agccaagatt cgaggctaca agaaggggcg cttcagtttc aacgtcaagg
    32221 gaggtcgctg tgaagcttgc tcaggtgacg ggattatcaa gattgagatg cacttcttgc
    32281 cagatgttta tgtggcttgt gaagtttgcc acggaacccg ctataacagt gaaaccctag
    32341 aagttcacta caaggaaaag aatatttcgc aggtcttgga tatgacggtc aacgatgcgg
    32401 tagaattttt ccaacacatt ccgaaaattc aacgcaaact ccagaccatc aaggatgtag
    32461 gtctaggcta tgtgaccttg ggacagccag ctaccaccct ttctggggga gaagcccaac
    32521 gtatgaagtt agctagcgaa ctccacaaac gctcgacagg aaaatctttc tacattctgg
    32581 atgagccgac gacagggctt catacagagg acattgctcg cctgctcaag gttttagctc
    32641 gctttgtaga cgatggcaat acagtcctcg tcatcgagca caatctggat gttatcaaga
    32701 cggcagacca tatcattgac ttgggacctg agggcggtgt cggtggtgga accatcatcg
    32761 taacaggaac tccagaagaa gtagctgcca acgaagccag ctatacagga cactatttga
    32821 aaggaaagtt acatcatgga taaacgcgta caagcatttc tagctaaaat gcaagaaaaa
    32881 gaactagatg gtatcatcat caacaatctt aaaaacgtct attatttgac tggtttttgg
    32941 ggctcaaacg gaacagtctt tatcagccgt gaccgtcagg tcttagtgac agactctcgc
    33001 tatatcatcg cagctaagca agaaaccagt ggttttgaga ttgtggctga tcgtgatgaa
    33061 ttggctgtca ttgcaggaat tgttaaggac atgggcttga ctcgtatcgg ttttgaagat
    33121 gagatttcag tgtcttatta ccaccgtatg caggcagctt ttgcaggttt ggacttgctt
    33181 ccacaaactc agtttgtgga aggtcttcgt atgattaagg atgaggcaga gattgcagcg
    33241 attcgcaagg cttgttctat ctcagaccaa gctttccgcg atgcgcttga ctttatcaaa
    33301 ccaggaaaaa ctgaaattga gattgccaac ttccttgatt tccgcatgcg tgagttggga
    33361 gcatctggct tatcttttga tacgatccta gctagcggta tcaattcttc taaaccccat
    33421 gcccatccaa tgcacaaacc agtggagttg ggagaagcca ttaccatgga cttcggctgt
    33481 ctctatgacc actatgtcag tgatatgacc cggactatct atctagggca tgttagcgat
    33541 gagcaggcag agatttacaa tacggttcta aaagctaacc aagccttgat tgaccaagct
    33601 aaggcaggct taggtttccg tgactttgac aaaatccctc gtgatattat cattgaggca
    33661 ggttatggtg actactttac tcacggcatt ggccacggta ttggtctgga tatccatgag
    33721 gaaccctact ttagccagac ttctacagaa actattaaga caggtatggc cttgaccgat
    33781 gaaccaggta tctatatcga aggcaaatat ggcgttcgta tcgaggatga tatcctgatt
    33841 acagagacag gttgtgaatt attgacccta gctccaaaag agttgatagt catttagttg
    33901 tttgtcaaga aaaaagtacg aaaatgacga aaaaagtcaa aaaaaattaa aaataggtcg
    33961 caagtcggat gttttttatg gtataataga ctaaactgat agtaacatgt agcgaaaggg
    34021 gtaggtacat gattaaaatt tatacagtct caagttgtac tagctgtaaa aaagcaaaaa
    34081 cctggctcaa tgcccaccag ttaagttata aagaacaaaa ccttggtaaa gaaggaatta
    34141 cgagagaaga attactggat attctaacca aaacagataa cggaatagcc agcatcgttt
    34201 cgtctaaaaa tcgctatgcc aaagcccttg gagtggatat tgaagatttg agtgtcaatg
    34261 aagttctcaa tctgattatg gaaacaccga gaattttaaa gagcccaatc cttgtagatg
    34321 aaaaacgcct gcaagttggc tacaaggaag acgatattcg tgccttccta ccacgctctg
    34381 tccgtaatgt agaaaatgca gaagcacgtt tgcgtgcagc tctataaaca tcaaggctgg
    34441 gagcacttcc cagtcttatt ctattttaat ttcaaaaaga aagaagaaag aaatgaaaaa
    34501 aatagttctt gttagtctag ctttcctttt tgtcctggtt ggttgcggac agaaaaaaga
    34561 aactggacca gctacaaaaa cagaaaaaga tacgcttcag tcggcattgc cagttattga
    34621 aaatgctgag aagaatacag ttgtaactaa gactttggtc ttgcccaagt cagatgatgg
    34681 tagccagcaa actcaaacca ttacatacaa ggacaagact tttttgagct taacgattca
    34741 acaaaaacgt ccagtttctg atgagttgaa gacttatatt gaccaacatg gagtggaaga
    34801 aactcaaaaa gctcttcttg aagcggagga gaaggataag tctatcattg aagctcgtaa
    34861 attggtaggt ttcaaacttg aaacaaaact attgagcgca acggaacttc aaacaacgac
    34921 tagttttgat tttcaagttc tggatgtcaa gaaggcttcc cagttggaac atctgaagaa
    34981 tattggtttg gaaaatcttt tgaaaaatga accaagcaac tatatttcag acagattggc
    35041 aaatggtgcg acagaacagt agaaaatcca aataaataga ctgcctgaat tgtagaaggt
    35101 aaggaattat gctataatgg tactattcta aggaaagagg gtgttagaat gggatttact
    35161 gaagaaacag tacgttttaa attggacgat tccaataaaa aagaaattag cgaaactttg
    35221 acagatgttt atgcttcgtt gaacgataag ggttacaacc caattaacca aatcgtaggt
    35281 tacgtattga gtggagaccc tgcctacgtt cctcgttata ataatgcacg aaatcaaatc
    35341 cgtaagtatg agcgtgatga aatcgttgag gaattggttc gctactacct caaaggacaa
    35401 ggagtcgatc tataacctat gagaattatg ggattggacg tcggttcaaa aacggtaggg
    35461 gtggcgatta gcgatccgct tggttttaca gctcaagggc ttgaaatcat ccagataaat
    35521 gaagaacaag gccaatttgg ttttgaccgc gttaaggaat tggttgatac ttacaaggtg
    35581 gaacgatttg tagtgggctt gcctaaaaac atgaacaata caagtggacc gcgcgtagaa
    35641 gctagtcaag cctacggagc aaagctagaa gagttttttg gtttaccagt agactatcag
    35701 gatgaacgct tgacaacagt ggctgctgag cgcatgttga ttgaacaagc agatatcagt
    35761 cgcaataagc gcaagaaagt cattgataag ttagcagctc agctgatttt acaaaattat
    35821 ttagatagaa aattttaata taaaggagag gctatgtcac acgatcataa ccacgaccac
    35881 gaagaacgtg aactaatcac actagtagat gagcaaggaa atgaaacctt gtttgaaatc
    35941 cttttgacaa ttgatggaaa agaagaattt ggtaaaaact atgttcttct agtaccagtt
    36001 aacgcagaag aagacgaaga cggacaagtt gaaatccaag cttactcatt catcgaaaac
    36061 gaagatggaa cagaaggcga attgcaacca atcccagaag actcagaaga cgaatggaac
    36121 atgattgaag aagtcttcaa cagctttatg gaggagtaaa aacgtccggg ggacgttttt
    36181 agccctgcgc tagaaattag aaacgcaggt agtctgagag actgtatcag cccaactccc
    36241 agaaatgggg aagagttgga acgtccagtg aacattttta ccccaactcc tagaaattga
    36301 gaagagttgg aacatctagt ggatattttt agcccacgtt cacattcata gtagctagtt
    36361 attagctaac caggtaagag gtcgggacga aaatcctggc cttgttttta ttagtaacta
    36421 tactttaatg cgataattga ttggactttt gtcaaggaga tgtgtatgtt tgaagtagaa
    36481 gaatggcttc atagtcggat tggtttgaat tttcgatcag gtttgggccg aatacagcaa
    36541 gcggtggatt tgttaggaaa tcctgagcag tcttacccta ttatccacgt aacagggact
    36601 aatgggaaag gatctaccat tgcttttatg agggaattat ttatggggca tggcaaaaaa
    36661 gttgcgacct ttacctcccc tcatatcgta tccatcaatg accgaatttg cattaatggg
    36721 caacctatag cagacgcaga ctttatccgt ttggctgatc aggtcaagga gatggagaaa
    36781 acgcttctgc aaactcctga ccagttgtcc ttttttgaat tgctgacttt ggttgctttt
    36841 ctttatttta gggagcagga ggtggatttg gttttattag aagtgggaat tggtggctta
    36901 cttgacacga ccaatgtggt aactggagag cttgctgtca tcacctccat tgggcttgac
    36961 catcaagaaa ccttgggtga tagtctagaa gcaattgcag agcagaaagc tggtattttc
    37021 aaggctggta agaaggcagt gattgcgaaa ttgcctccag aagctaggct tgtctgtcag
    37081 aaaaaagccg aatctttagc tgttaacctt tatcaggcag gtcaagattt tttaatgctg
    37141 aatggtgatt tttcaagctc tttactaaat ctttcacagc tgaacatagg cttagaagga
    37201 gtctatcagc aggagaatgc agccttggcg ttgcaaactt ttcttctttt tatgggagaa
    37261 ggaaaggaag ctgttgatga acaggctgta agaaaggcct tggaacagac ccattgggct
    37321 ggtcgcttgg agcgtattcg cccacagatt tacttggatg gtgctcataa cctccctgcc
    37381 ttgactcgct tggttgagtt tatcaaagaa aaagagcagg aaggttatcg tcctcaaatc
    37441 ctttttggag ccttgaaacg gaaggattat caagggatgt taggttacct gactgaaaaa
    37501 ttgcctcagg tggaactcaa ggtgaccggc tttgactatc agggggcttt ggacgaaagg
    37561 gatgtgacag gttacgatat agtttcttct taccgagaat ttatcagcga ttttgaagaa
    37621 agagcagatg ctcaggattt gctgttcgtt acagggtctc tctattttat ctcagaagta
    37681 cagggctacc tgctggaccg tgagcagata aattgacccc cttgtttgaa cttgttatac
    37741 taggggaatt gctggttaga gaaaatttct ctaaattggt agcagaaagg aaattcatca
    37801 tgaaattaaa aagattcaca ctttctcttg cttctctagc aagttttagt ctcttagtag
    37861 cttgttcaca aagagctcaa caggttcaac agcctgctca acagaatacc aatactgcaa
    37921 atgcaggagg taaccaaaat caagcggctc cagtacaaaa ccaacctgtt gctcaaccga
    37981 ccgatattga tgggacttat actggtcagg atgacggaga ccgtatcact ttagtggtaa
    38041 ctggaacgac tggtacatgg actgagctcg aatctgacgg ggatcagaaa gtcaaacagg
    38101 ttacatttga ttcagcaaat caacgcatga ttattggcga tgatgtcaaa atttacactg
    38161 taaacggtaa tcaaatcgtc gtagatgata tggatagaga cccatcggac caaatcgttt
    38221 taactaaata agactatgca agtagaaagg gctggatttt tcagcctttt tacttttacc
    38281 tagaaaataa tcataaatac ttgccttcta ccgaatacct gcgttatact agtatcaatt
    38341 acttgttttt agcattcaaa ctatcaagga ggggatatga aatataggaa atttcaatta
    38401 ttgatgtcca agtatggctt tagtctttcg attatgctac ttgaactttg tcttgttttt
    38461 ggtctctttc tttatttagg acgcatggct cccattttat ggattactgt cctcattcta
    38521 ctgagtatca tcacaatcat ttcgatagtc aaccgtaata cgactcctga gaataaggta
    38581 acctggttgt tagtagcctt tgtgccagta tttggtccct tgctctatct gatgtttggt
    38641 gaaaggcgat tgtccaaaaa agaaatcaaa caactgaaga agctaggctc tatgcatttc
    38701 caagaagcaa atagccagct actaaaagag aaattaaaag aaagtgacaa ggcagcttat
    38761 ggagtcatca agtccttatt gagtatggat accaatgctg acatctatga tcaaactgcc
    38821 tctacatttt ttcctaacgg agaagctatg tggaaaaaga tggtagaaga tcttaaaaag
    38881 gctgagaaat ttattttctt ggaatattac attatagaag aaggtttgat gtggaatcgc
    38941 atactagata tactagagca aaaggtagct cagggtgtag aggttaagat gctctatgat
    39001 gatatcggct gtatggctac tttaacagga gattatgcac atcgacttcg tcagctgggc
    39061 atcgaggccc ataaattcaa taaagttatt cctcgtttga cagtggctta taataacaga
    39121 gatcatagaa aaatattgat tgttgatggt cagatagcct atactggtgg gatcaatctg
    39181 gcagatgagt acattaacca cgtcgagaga tttggttatt ggaaggatag tggaattcgc
    39241 ttagacggac tagcagtaaa agctctgaca cgcttatttt tgaccacttg gtacattaat
    39301 cgaggagaaa ttagtgattt tgatcaatat catttagaaa atcattctat cccgagtgac
    39361 ggtttaacca ttccatacgg aagtggaccc aagccaattt ttcgagcgca ggtagggaaa
    39421 aaagtttatc agagtttaat caatcaagca acagaatcgg tctatattac gacaccttat
    39481 ttgattatag attatgattt aacagagaca atcaaaaatg cagctatgag aggggtcgat
    39541 gttcgaatta tcacccctta cataccagat aagaagttca ttcagttagt cacgagagga
    39601 gcttatcccg accttctttc tgctggtgtt cggatttatg agtatagtcc aggttttatt
    39661 catagtaagc agatgttggt agacgaagat tttgcggtgg tggggacaat caatctcgac
    39721 taccggagct tggtacacca ttatgaaaat gcagtcttac tctataaaac tccttctata
    39781 agggaaatcg cccgagattt tcgaaatata tttgcagatt ctcaggaagt ctatcctcat
    39841 tctatcaaaa cgagctggta tcaaaagctt gtaaaagaaa tcgcccagct attcgcccct
    39901 atcttataag aaatctagaa cttaggatac aaaccagacc tatttttctt gtcttttacg
    39961 aatagaaaga aagaggaggg gaattcatgc taactaagga agaagtgaat gatatgattg
    40021 agttcaaaca gacacattta caccgctttc gggaaattga aacctttgtg aaactatgca
    40081 aaaaatcact caaacagcca tcgcaagctg acaatcctag gactttttga tatactaaga
    40141 cagataaatt gaacaggaga aacgatatga gtgtgtatgg tagagtagaa gaagttcata
    40201 aggagaatcg tgaacctcta gaataccaaa tcgaacaaga atcgcatcat cgtgaatcaa
    40261 gtcgtcttcc tttggtgaaa attttactat ggagtacgct tgtaacgggg ataactctag
    40321 gagtaccgct attactcgac ttaatgagtg cacaagaagt gcaggatttt tatgcaggtt
    40381 gggcgcttca tcagacaggg aagatttaca gcgactatta tggaagtcaa ggtttgcttt
    40441 attatttgct gacttacgtg agtcagggcg gatttttctt tgccatcttt gagtggttag
    40501 ccttggtagc aggaggattt ttccttttta gatcagcgga caccttgaca gagcaaggag
    40561 accaagctgg acatctggtg actatttttt acatgctagt tacaggtctt gcttttggtg
    40621 gaggctatgc gactctttta gcgcttcctt tcttattcgc agcctttagt ttagttgcgg
    40681 cttacctaag caatccaagc catgataagg gatttgtacg gattgggcta gctttggcag
    40741 gcggattttt ctttgctccc ttatcatcgc tcctgtttat tgctgtagtg agtttaggct
    40801 tgttggtctt taaccttggg catagacgct ttgcgcatgg gttttatcag tttcttgcag
    40861 tggctttagg tttttcactt gtcttttatc caactgccta ctatagtgct gcaacaggaa
    40921 gttttgggga tgcgattagt ggtattcgtt atcctattga cagtattcgc tttgatttta
    40981 cttctaaaat tttagagaat atgttttttt atggcttgtt gtcccttggt ttgggatttg
    41041 tggtttgtat ctttttaggt ctctttcaat ccaaaccctt taaactatac gtcatttcgg
    41101 ttcctgcaag tcttgtggta attttaggtt tgattttact tttcttttca caagagcctc
    41161 tgcacgcttc ttatttgatg gtcgtcttcc ctgttttcct acttttattg gtaaccaata
    41221 ttaagagtca acagaggggg cgtagtgcta gaagaagccg aagagaaacg ccagttagcc
    41281 tatggagtcg tttcttcaaa ggaaatctat atctgctagt ttttgggttt gtctatcttt
    41341 tgtctgttcc ttttttgatg aagtttgtcc tttatccagt accttatcaa gaacgtaatc
    41401 gtcttgctga tttggtaaaa gaggagacaa atacggaaga tgctatctat gcatgggatg
    41461 atactgcgac tctttatcgt aagagtgagc gcttgtcgcc atcggcgatt ttgtccccgt
    41521 tgcactatac agcaactgag gaaaatcgta ataagttact taatgacttg aaagaaaaac
    41581 aacctaaggt gattgtggta aatgacaagg tggaagtctg gtctgaagtg gaaacactct
    41641 taaaagaaaa ttaccaacaa gtaaagactg attactcaga gtttaaagtc tataaaatta
    41701 aataaccaaa tcaatatctt gtgtattttt aaaaatttta agatttttaa cacaagatat
    41761 tgatttttct ttttagagtg gtataatact ttttagaaag aacattttag aaaagagcat
    41821 gcatatgatt gcactagaag aaaaaattac aattttgcca actctcttcg tcgagaaacg
    41881 agatgggaga cgtgttgtat ttgatgtgga caagattgac aaggctctcc acaaggcagc
    41941 tgacaaggtt atggatgtga cacccctggt tgaaaaacgc ctcaatgatc tgactgagag
    42001 aattattaca gaaattcata gtcgctttcc acagggaatt aagatttacg aaattcaaaa
    42061 tatcgtagaa catgaactcc ttgaagccaa agaatatgcg ctggctgagg agtatattac
    42121 ttatcggaca cagagggatt ttgagcgctc aaaagcgacg gatatcaact ttagtattca
    42181 taaacttctc aacaaagacc agacagttgt caatgaaaat gctaataaag acagtgatgt
    42241 ctttaacact cagcgtgatt tgacagcagg gattgttggg aaatcaatcg gactgcaaat
    42301 gcttcctaag cacgtagcca atgcccacca aaagggggat atccactatc acgatttgga
    42361 ctacagtccc tataccccta tgaccaactg ctgtttgatt gattttaagg gtatgttgga
    42421 aaatggtttt aagattggaa atgcagaggt agagagtccc aagtctatcc agactgcgac
    42481 agcacagatt tctcaaatca ttgccaacgt tgcttctagc cagtacggtg gctgttcagc
    42541 tgaccgtatc gatgaaattt tggcgcctta tgcagagaag aactatcaaa aacatctcaa
    42601 agatgcagaa gagtgggtat tgcctgaaaa acgggaagat tacgcttgga agaaaacgca
    42661 aaaggacatt tacgatgcca tgcaatctct tgagtatgaa atcaatactc tcttcacttc
    42721 aaatggacaa acacctttta cttcgctagg ttttggtctg ggaaccagtc gttttgaacg
    42781 agaaattcaa aaagctattt taaacattcg catcaagggt cttggttcag aacaccgtac
    42841 ggctatcttt cctaaactta ttttcactct gaaaagaggc cttaatttag aggaaggaac
    42901 tcccaactac gacatcaagc agttggctct tgagtgtgca accaaacgga tgtatccaga
    42961 tgttttatct tacgataaga ttgttgactt gacaggatcc ttcaaggtgc ctatgggttg
    43021 ccgttctttc ctccaaggat ggaaggatga aaatggtgta gaagtcaatt caggtcgcat
    43081 gaatctgggt gttgtgacgg ttaatctgcc tcgtattgct cttgagtctg aaggtgatat
    43141 gaataagttc tgggaaatct tcaacgagcg aatgaatatc gcagaagatg ctcttgttta
    43201 ccgtgtcgaa cgcactaaag aggcgacacc agcgaatgct cctatccttt atcagtacgg
    43261 tgcttttggc catcgtctag gtaaagaaga aagtgttgac cagctcttta agaatcgtcg
    43321 tgcgaccgtt tcgctgggct atatcggctt gtatgaagta gcgacagttt tctttggtaa
    43381 cagctgggaa agtaatccag atgctaagga attcacgcta gacatcattc acgatatgaa
    43441 acgccgtgta gaagagtggt cagaccaata tggctaccat ttctctatct actcaacacc
    43501 atccgaaagt ctgacagacc gtttctgccg actagatata gacaagtttg gctctattcc
    43561 tgatatcaca gacaaggaat actacaccaa ctctttccac tacgatgttc gtaaaaatcc
    43621 aacaccgttt gaaaaattgg actttgagaa agtctatcca gaagcaggtg cgtcaggtgg
    43681 tttcatccat tattgtgagt atccagtcct tcagcaaaat ccaaaggcct tggaagctgt
    43741 ctgggattat gcttatgacc gtgtaggcta tctaggcacc aatactccga ttgaccgttg
    43801 ctacaagtgt gactttgaag gggattttga accaactgag agagggtttg cttgtccaaa
    43861 ctgtggcaat agcgacccta aaacagtaga tgtggtgaag cgaacttgtg gctacctagg
    43921 taatcctcaa gcaagaccga tggtcaacgg gcgtcacaag gaaatcgctg cgcgtgtcaa
    43981 acatatgaat ggttcaacga ttaaaatagc tgggcatcaa gtaacaaatt agaaagaaat
    44041 gaaatgggaa aatatcaact agacgataag gggcgcgcac aagtgacccg ttatcacgag
    44101 aaacactcta aaggtggagc tggtaagaaa gaacgcttgc ttagcttcag agaacaattt
    44161 ttaaacaaga acaagaaaaa ataaaagtga gagccagctc tcgcttttct catagtggga
    44221 ggtaaggatg gaattacgca gaccaagatt agcggataag aaagctgttt tagatatgat
    44281 gacagagttt gaaaagagcc aatcagccca tgatggagga ttttgggata cagagaattt
    44341 tgtgtatgaa gagtggttgg aaagcaatca ggaacaggaa atggggatta atctgcctga
    44401 aggatgggtt cctgcaattc agttagtggc tttttctgag aaaggtcaag cagttggatt
    44461 tcttaatctc cggttgcgcc tcagtaactt tctactagaa gaaggtgggc acattggcta
    44521 ctccatccgt ccatctgaaa gaggcaaggg ttatgcaaaa gagactctcc gtcagggctt
    44581 gcaagttgct aaggaaaaga acatcaagaa agctctggtg acctgtagcg tgaataatcc
    44641 tgctagcaga gcagtcattc tagcaaatgg tggaatattt gaggatgctc acaatggagt
    44701 cgagcgttat tggatagagg tagcgaatga ataatccaaa accacaagaa tggaaaagcg
    44761 aggaacttag tcaaggtcgt atcattgact acaaggcctt taactttgtt gatggcgaag
    44821 gcgtgcgtaa ctctctctat gtatcaggct gtatgtttca ctgcgaggga tgttataatg
    44881 ttgcgacttg gtcttttaat gctggcattc tctatacagc agaattagaa gagcagatta
    44941 tggcagacct tgcccaaccc tatgttcaag gcttgacttt gctgggaggg gagccttttc
    45001 ttaatactgg cattctcttg cctctagtta aacgcatccg aaaggaattg ccagacaagg
    45061 acatttggtc ctggaccggc tacacttggg aagaaatgat gttggaaact ccagataaac
    45121 tggaactctt gtcactgatt gacattcttg tcgatggaag atatgatcga actaagagaa
    45181 atcttatgct ccagtttcga ggttcgtcta accaacgaat tatcgatgtg caaaaatcgc
    45241 tcaaaagtgg gcaagtagtg atttgggaca agctcaatga cggaaaagaa agctatgaac
    45301 aggtgaagag agaatgaaga aaaaggactt agtagaccaa ctagtctcag agatcgagac
    45361 ggggaaagtc aggacactgg gaatatacgg tcatggagct tcaggtaaat caacctttgc
    45421 acaggaattg taccaagctt tagattctac tacagtaaat ttgctagaga cagatcctta
    45481 tatcacctca ggacgccatc tggtagtacc caaggacgcg ccgaatcaaa aggtgacagc
    45541 cagtctgcca gtggcgcatg aactggagag tttgcagaga gatatccttg ccttgcaggc
    45601 gggtatggat gtcttgacaa ttgaagaacc ttggaaggct agtgaggtct tgtctggagc
    45661 caaaccaatt ttgattgtcg aagggatgtc tgttggcttt ctacccaagg aactctttga
    45721 aaaaaccatc tgtttctaca cggatgagga gaccgaatta aagcgacgcc ttgctagaga
    45781 tacgactgtg agaaatcgtg atgcatcctt tatattggct agccatcaga tgagacggga
    45841 gcagtatctg cgatactata aagaaactga gtctaaggcg gatatcttag tggatcaatc
    45901 agaagataaa tttgatgtca agaggactta acttatgtat agaagaaata tagaagaaaa
    45961 tccctaattt taggaagaaa aagtaggaaa acagtttcat cccaaaaaaa cgaaaaaacc
    46021 taacaaatcc cttgcaatcg caggggattt gtgttattct attatggtgc tgtaaattac
    46081 agccttagtt ttgatacaag aggttgcgac acgctcggtt gcattgccac gcaacaccgt
    46141 gtcggttttc ttgtggagct agcctattat cttaaataga cgaaaaggag aaaaagatgg
    46201 caaacaaaaa aatccgtatc cgtttgaaag cttacgaaca ccgtacgctt gacacagcgg
    46261 ctgcaaaaat cgtagaatca gctactcgta caggtgcaca agttgcgggt ccaatcccac
    46321 ttccaactga acgtagcctc tacacaatca ttcgtgcgac tcacaaatac aaagactctc
    46381 gcgaacaatt tgaaatgcgt acacacaaac gtttgatcga tatcgttaac ccaactcaaa
    46441 aaacagttga tgccttgatg aaattggatc ttccaagtgg tgtaaacgta gaaatcaaac
    46501 tttaatctaa aatataaaag agcagaggct ggtgtttcaa tctaattgaa cacggactaa
    46561 actcggtgtg aaaaagataa acttcctatt gtccgttaga cactgcgtta gtttcctatt
    46621 ttcactttga gtttgacgcc ctttgtatct tagacttgag cataaaaaac gctcgttaaa
    46681 aactttttga ataaaaaata tagaaaagga actattttct catgacaaaa ggaatcttag
    46741 ggaaaaaagt gggaatgact caaatcttca ctgaagctgg cgaattgatc cctgtaacag
    46801 ttattgaagc aactccaaac gttgttcttc aagttaaaac tgttgaaaca gacggataca
    46861 acgctatcca agttggtttc gatgacaaac gcgaagtatt gagcaacaaa cctgctaaag
    46921 gacatgtagc gaaagctaac acggctccta agcgcttcat tcgtgaattc aaaaacgttg
    46981 aaggcttgga agttggcgct gaaatcacag ttgaaacatt cgcagctgga gatgttgttg
    47041 atgtaactgg tacttctaaa ggtaaaggtt tccaaggtgt tatcaaacgc cacggacaat
    47101 cacgtggacc aatggctcac ggttctcgtt accaccgtcg tccaggttct atggggcctg
    47161 ttgcacctaa ccgcgtattc aaaggtaaaa accttgcagg acgtatgggt ggtgaccgcg
    47221 taacaattca aaatcttgaa gttgtacaag ttgttccaga aaagaacgtt atccttatca
    47281 aaggtaacgt accaggtgct aagaaatctc ttatcactat caaatcagca gttaaagctg
    47341 gtaaataata aagaaagggg aaatcagtca caatggcaaa cgtaacatta tttgaccaaa
    47401 ctggtaaaga agctggccaa gttgttctta acgatgcagt atttggtatc gaaccaaatg
    47461 aatcagttgt gtttgatgta atcatcagcc aacgcgcaag ccttcgtcaa ggaacacacg
    47521 ctgttaaaaa ccgctctgca gtatcaggtg gtggacgcaa accgtggcgt caaaaaggaa
    47581 ctggacgtgc tcgtcaaggt tctatccgct caccacaatg gcgtggtggt ggtgttgtct
    47641 tcggaccaac tccacgttca tacggctaca aacttccaca aaaagttcgt cgcctagctc
    47701 ttaaatcagt ttactctgaa aaagttgctg aaaacaaatt cgtagctgta gacgctcttt
    47761 catttacagc tccaaaaact gctgaatttg caaaagttct tgcagcattg agcatcgatt
    47821 ctaaagttct tgttatcctt gaagaaggaa atgaattcgc agctctttca gctcgtaacc
    47881 ttccaaacgt gaaagttgca actgctacaa ctgcaagtgt tcttgacatc gcaaatagcg
    47941 acaaacttct tgtcacacaa gcagctatct ctaaaatcga ggaggttctt gcataatgaa
    48001 tttgtatgat gttatcaaaa aacctgtcat cactgaaagc tcaatggctc aacttgaagc
    48061 agggaaatat gtatttgaag ttgacactcg tgcacacaaa cttttgatca agcaagctgt
    48121 tgaagctgct ttcgaaggtg ttaaagttgc caatgttaac acaatcaacg taaaaccaaa
    48181 agctaaacgt gttggacgtt acactggttt tactaacaaa actaaaaaag ctatcatcac
    48241 acttacagct gattctaaag caatcgagtt gtttgctgct gaagctgaat aatctaagga
    48301 ggaaatatcg tgggaattcg tgtttataaa ccaacaacaa acggtcgccg taatatgact
    48361 tctttggatt tcgctgaaat cacaacaagc actcctgaaa aatcattgct tgttgcattg
    48421 aagagcaagg ctggtcgtaa caacaacggt cgtatcacag ttcgtcacca aggtggtgga
    48481 cacaaacgtt tctaccgttt ggttgacttc aaacgtaata aagacaacgt tgaagcagtt
    48541 gttaaaacaa tcgagtacga tccaaaccgt tctgcaaaca tcgctcttgt acactacact
    48601 gacggtgtga aagcatacat catcgctcca aaaggtcttg aagtaggtca acgtatcgtt
    48661 tcaggtccag aagcagatat caaagtcgga aacgctcttc cacttgctaa catcccagtt
    48721 ggtactttaa ttcacaacat cgagttgaaa ccaggtcgtg gtggtgaatt ggtacgtgct
    48781 gctggtgcat ctgctcaagt attgggttct gaaggtaaat atgttcttgt tcgtcttcaa
    48841 tcaggtgaag ttcgtatgat tcttggaact tgccgtgcta cagttggtgt tgtcggaaac
    48901 gaacaacatg gacttgtaaa ccttggtaaa gcaggacgta gccgttggaa aggtatccgc
    48961 ccaacagttc gtggttctgt aatgaaccct aacgatcacc cacacggtgg tggtgaaggt
    49021 aaagcaccag ttggtcgtaa agcaccatct actccatggg gcaaacctgc tcttggtctt
    49081 aaaactcgta acaagaaagc gaaatctgac aaacttatcg ttcgtcgtcg caacgagaaa
    49141 taatattaaa ctagtcgctt aagcaactag taaatccgcc agctcggtag cgctccatag
    49201 gagtgcaagc cgctgtggta caacatttaa aggagaaaat ataaaaatgg gacgcagtct
    49261 taaaaaagga cctttcgtcg atgagcattt gatgaaaaaa gttgaagctc aagctaacga
    49321 cgaaaagaaa aaagttatta aaacttggtc acgtcgatca acgatcttcc caagtttcat
    49381 tggttacact attgcagttt atgacggacg taaacacgta cctgtttaca tccaagaaga
    49441 catggtaggc cacaaacttg gtgaatttgc accaactcgt acttacaaag gtcacgctgc
    49501 agacgacaag aaaacacgta gaaaataagg agaacataaa tggcagaaat tacttcagct
    49561 aaagcaatgg ctcgtacagt acgtgtttca cctcgtaaat cacgtcttgt tcttgataac
    49621 atccgtggta aaagcgtagc cgatgcaatc gcaatcttga cattcactcc aaacaaagct
    49681 gctgaaatca tcttgaaagt tttgaactca gctgtagcta acgctgaaaa caactttggt
    49741 ttggataaag ctaacttggt agtatctgaa gcattcgcaa acgaaggacc aactatgaaa
    49801 cgtttccgtc cacgtgcgaa aggttcagct tcaccaatca acaaacgtac agctcacatc
    49861 actgtagctg ttgcagaaaa ataaggaggt aaaatcgtgg gtcaaaaagt acatccaatt
    49921 ggtatgcgtg tcggcatcat ccgtgattgg gatgccaaat ggtatgctga aaaagaatac
    49981 gcggattacc ttcatgaaga tcttgcaatc cgtaaattcg ttcaaaaaga acttgctgac
    50041 gcagcagttt caactattga aatcgaacgc gcagtaaaca aagttaacgt ttcacttcac
    50101 actgctaaac caggtatggt tatcggtaaa ggtggtgcta acgttgatgc actccgtgca
    50161 aaacttaaca aattgactgg aaaacaagta cacatcaaca tcatcgaaat caaacaacct
    50221 gatttggatg ctcaccttgt aggtgaagga attgctcgtc aattggagca acgtgttgct
    50281 ttccgtcgtg cacaaaaaca agcaatccaa cgtgcaatgc gtgctggagc taaaggaatc
    50341 aaaactcaag tatcaggtcg tttgaacggt gcagatatcg cccgtgctga aggctactct
    50401 gaaggaactg ttccgcttca cacacttcgt gcagatatcg attacgcttg ggaagaagca
    50461 gatactacat acggtaaact tggtgttaaa gtatggatct accgtggtga agttcttcca
    50521 gctcgtaaaa acactaaagg aggtaaataa ccaatgttag tacctaaacg tgttaaacac
    50581 cgtcgtgaat tccgtggaaa aatgcgcggt gaagcaaaag gtggaaaaga agtagcattc
    50641 ggtgaatacg gtcttcaagc tacaactagc cactggatca ctaaccgcca aatcgaagct
    50701 gctcgtatcg ccatgactcg ttacatgaaa cgtggtggta aagtttggat taaaatcttc
    50761 ccacacaaat catacactgc taaagctatc ggtgtgcgta tgggatctgg taaaggggca
    50821 cctgaaggtt gggtagcacc agttaaacgt ggtaaagtga tgttcgaaat cgctggtgta
    50881 tctgaagaga ttgcacgtga agcgcttcga cttgctagcc acaaattgcc agttaaatgt
    50941 aaattcgtaa aacgtgaagc agaataagga gaaggcatga aacttaatga agtaaaagaa
    51001 tttgttaaag aacttcgtgg tctttctcaa gaagaactcg cgaagcgcga aaacgaattg
    51061 aaaaaagaat tgtttgaact tcgtttccaa gctgctactg gtcaattgga acaaacagct
    51121 cgcttgaaag aagttaaaaa acaaatcgct cgtatcaaaa cagttcaatc tgaagcgaaa
    51181 taatagacta gggaaggaga aatttcaatg gaacgcaata atcgtaaagt tcttgttgga
    51241 cgtgttgtat ctgacaaaat ggacaagaca atcacagttg tagttgaaac aaaacgtaac
    51301 cacccagtct atggtaaacg tattaactac tctaaaaaat acaaagctca tgatgaaaac
    51361 aatgttgcca aagaaggtga tatcgtacgt atcatggaaa ctcgcccgct ttcagctaca
    51421 aaacgtttcc gtcttgtaga agttgttgaa gaagcggtca tcatctaatc aaacctgaaa
    51481 ggagaaaact gaaatgattc aaacagaaac tcgtttgaaa gtcgcagaca acagcggtgc
    51541 tcgcgaaatc ttgactatca aagttcttgg tggttcagga cgtaaatttg caaacatcgg
    51601 tgatgttatc gtggcatctg taaaacaagc tactcctggt ggtgcggtta aaaaaggtga
    51661 cgttgttaaa gcagttatcg ttcgtactaa atcaggtgct cgtcgtgctg atggttcata
    51721 catcaaattt gacgaaaacg cagcagttat catccgtgaa gacaaaactc ctcgcggaac
    51781 acgtatcttt ggcccagttg cacgtgaatt gcgtgaaggt ggtttcatga agatcgtgtc
    51841 acttgctcca gaagtacttt aatttttagg aacaaactag tcccctagct tcaagctagg
    51901 gtgcccttat gggcgtaaga aaaatcaagg agaaacctaa tgtttgtaaa aaaaggcgac
    51961 aaagttcgcg taatcgctgg taaagataag ggaacagaag ctgttgtcct tactgccctt
    52021 ccaaaagtaa acaaagttat cgttgaaggt gttaacattg ttaagaaaca ccaacgtcca
    52081 actaacgagc ttcctcaagg tggtatcatc gagaaagaag cagctatcca cgtatcaaat
    52141 gttcaagttt tggacaaaaa tggtgtagct ggtcgtgttg gttacaaatt tgtagacggt
    52201 aaaaaagttc gctacaacaa aaaatcaggc gaagtgcttg attaatcacg aaggaaagga
    52261 gaagtataat ggcaaatcgt ttaaaagaaa aatatcttaa tgaagtagtt cctgctttga
    52321 cagaacaatt caactactca tcagtgatgg ctgtgcctaa agtagataag attgttttga
    52381 acatgggtgt tggtgaagct gtatcaaacg ctaaaagcct tgaaaaagct gctgaagaat
    52441 tggcacttat ctcaggtcaa aaaccactta tcactaaagc taaaaaatca atcgccggct
    52501 tccgtcttcg tgaaggtgtt gcgatcggtg caaaagttac ccttcgtggt gaacgtatgt
    52561 acgaattctt ggataaattg gtatcagttt cacttccacg tgtacgtgac ttccacggtg
    52621 ttccaacaaa atcatttgat ggacgcggga actacacact tggtgtgaaa gaacaattaa
    52681 tcttcccaga aatcaacttc gatgacgttg acaaaactcg tggtcttgac atcgttatcg
    52741 taacaactgc taacactgac gaagagtcac gtgcattgct tacaggcctt ggaatgcctt
    52801 ttgcaaaata atataggagg taaatctaat ggctaaaaaa tcaatggtag ctagagaggc
    52861 taaacgccaa aaaattgttg accgttatgc tgaaaaacgt gctgcattaa aggcggcagg
    52921 ggactacgaa ggtttatcta aattacctcg caacgcctca ccgactcgtt tacataatcg
    52981 ttgtagagtt acggggcgcc cacattcagt ttaccgcaaa tttggtctga gtcgtatcgc
    53041 ttttcgcgaa cttgcgcata aaggtcaaat tcctggtgta acaaaagcat cttggtaatt
    53101 taagatatca agagcgtcaa aactccaagt aaaaatagga aacttgacga agaaactaaa
    53161 gtttctagga aagtttatct ttttcacaca gagtttagcc cgggttcaat tgggcttgcc
    53221 aatttgaaca cgagctacag ctttggaaaa aaagaccaat ttgctttgga gcattgcttc
    53281 tgcattaaat tgtctatttt tgctcgtgct gttacgctct ttgtatcatg tattaactag
    53341 caagtgcaac ttgcaaacta ctagtaagag gagaaaaaca aaatggttat gactgaccca
    53401 atcgcagact tcctaactcg tattcgtaat gctaaccaag ctaaacacga agtacttgaa
    53461 gtacctgcat caaacatcaa aaaagggatt gctgaaatcc ttaaacgcga aggttttgta
    53521 aaaaacgttg aaatcattga agatgacaaa caaggcgtca tccgtgtatt tcttaaatac
    53581 ggaccaaatg gtgagaaagt tatcactaac ttgaaacgtg tttctaaacc aggacttcgt
    53641 gtctacaaaa aacgtgaaga ccttccaaaa gttcttaacg gacttggaat tgccatcctt
    53701 tcaacttctg aaggtttgct tactgataaa gaagcacgcc aaaagaatgt tggtggtgag
    53761 gttatcgctt acgtttggta aaatcaagat acaaagctcg taaagaacaa agcaaaatta
    53821 ggaagttgga gaagtttgtt tacaaacaag ccaacttatc tattttgcac agttcttaga
    53881 gcgtgttcag ttcagctctt gaactaaata agtatctgaa ccccgtgaaa actggccgtt
    53941 ctggcctgac aatttaacag gagaaaataa acatgtcacg tattggtaat aaagttatcg
    54001 tgttgcctgc tggtgttgaa ctcgctaaca atgacaacgt tgtaactgta aaaggaccta
    54061 aaggagaact tactcgtgag ttctcaaaag atattgaaat ccgtgtggaa ggtactgaaa
    54121 taactcttca ccgtccaaac gattcaaaag aaatgaaaac tatccacgga actactcgtg
    54181 cccttttgaa caacatggtt gttggtgtat cagaaggatt caagaaagaa cttgaaatgc
    54241 gtggggttgg ttaccgtgca cagcttcaag gatctaaact tgttttggct gttggtaaat
    54301 ctcatccaga cgaagttgaa gctccagaag gaattacttt tgaacttcca aacccaacaa
    54361 caatcgttgt tagcggaatt tcaaaagaag tagttggtca aacagctgct tacgtacgta
    54421 gccttcgttc accagaacca tataaaggta aaggtatccg ttacgttggt gaattcgttc
    54481 gccgtaaaga aggtaaaaca ggtaaataat gttgagtggt tgatcatcaa ccaccaacct
    54541 attttccaac tttgtgcata gcacacgatt taaaactaaa gaggtgaaaa ctgtgatttc
    54601 aaaaccagat aaaaacaaac tccgccaaaa acgccaccgt cgcgttcgcg gaaaactctc
    54661 tggaactgct gatcgcccac gtttgaacgt attccgttct aatacaggca tctacgctca
    54721 agtgattgat gacgtagcgg gtgtaacgct cgcaagtgct tcaactcttg ataaagaagt
    54781 ttcaaaagga actaaaactg aacaagccgt tgctgtcggt aaactcgttg cagaacgtgc
    54841 aaacgctaaa ggtatttcag aagtggtgtt cgaccgcggt ggatatctat atcacggacg
    54901 tgtgaaagct ttggctgatg cagctcgtga aaacggattg aaattctaat aggaggacac
    54961 tagaaaatgg catttaaaga caatgcagtt gaattagaag aacgcgtagt tgctgtcaac
    55021 cgtgttacaa aagttgttaa aggtggacgt cgtcttcgtt tcgcagctct tgttgttgtt
    55081 ggtgaccaca atggtcgcgt aggatttggt actggtaaag ctcaagaagt tccagaagca
    55141 atccgtaaag cagtagatga tgctaagaaa aacttgatcg aagttcctat ggttggaaca
    55201 acaatcccac acgaagttct ttcagaattc ggtggagcta aagtattgtt gaaacctgct
    55261 gtagaaggtt ctggagttgc cgctggtggt gcagttcgtg ccgttgtgga attggcaggt
    55321 gtggcagata ttacatctaa atcacttggt tctaacactc caatcaacat tgttcgtgca
    55381 actgttgaag gtttgaaaca attgaaacgc gctgaagaaa ttgctgccct tcgtggtatt
    55441 tcagtttctg atttggcata agaaagggga taaaatggct caaattaaaa ttactttgac
    55501 taagtctcca atcggacgca ttccatcaca acgtaaaact gttgtagcac ttggacttgg
    55561 caaattgaac agctctgtta ttaaagaaga taacgctgct atccgtggta tgatcacagc
    55621 agtatctcac ttagtaacag ttgaagaagt aaactaatga agttttaggg gatgtgcact
    55681 gtaccatccc ctaaaactag atatagtcat ctatgatgac atcgtatagg cgagttgatg
    55741 ggggagacaa ccttttctcc cttatcggcg ctagcatttt acaaaagagg agaaaataaa
    55801 aatgaaactt catgaattga aacctgcaga aggttctcgt aaagtacgta accgcgttgg
    55861 tcgtggtact tcatcaggta acggtaaaac atctggtcgt ggtcaaaaag gtcaaaaagc
    55921 tcgtagcggt ggcggagttc gccttggttt tgaaggtgga caaactccat tgttccgtcg
    55981 tcttccaaaa cgtggattca ctaacatcaa cgctaaagaa tacgcaattg tgaaccttga
    56041 ccaattgaac gtctttgaag atggtgctga agtaactcca gttgttctta tcgaagcagg
    56101 aattgttaaa gctgaaaagt caggtattaa aattcttggt aacggtgagt tgactaagaa
    56161 attgactgtg aaagcagcta aattctctaa atcagctgaa gaagctatca ctgctaaagg
    56221 tggttcagta gaagtcatct aagagaggtg acctatgttt tttaaattat taagagaagc
    56281 tcttaaagtc aagcaggttc gatcaaaaat tttatttaca atttttatcg ttttggtctt
    56341 tcgtatcgga actagcatta cagttcctgg tgtgaatgcc aatagcttga atgctttaag
    56401 tggattatcc ttcttaaaca tgttgagctt ggtgtcgggg aatgccctaa aaaacttttc
    56461 gatttttgcc ctaggagtta gtccctatat caccgcttct attgttgtcc aactcttgca
    56521 aatggatatt ttacccaagt ttgtagagtg gggtaaacaa ggggaagtag gtcgaagaaa
    56581 attgaatcaa gctactcgtt atattgctct agttctcgct tttgtgcaat ctatcgggat
    56641 tacagctggt tttaatacct tggctggagc tcaattgatt aaaactgctt taactccaca
    56701 agtttttctg acgattggta tcatcttaac agctggtagt atgattgtca cttggttggg
    56761 tgagcaaatt acagataagg gatacggaaa cggtgtttcc atgattatct ttgccgggat
    56821 tgtttcctca attccagaga tgattcaggg catctatgtg gactactttg tgaacgtccc
    56881 aagtagccgt atcacttcat ctatcatttt cgtaatcatt ttgattatta ctgtattgtt
    56941 gattatttac tttacaactt atgttcaaca agcagaatac aaaattccaa tccaatatac
    57001 taaggttgca caaggtgctc catctagctc ttaccttccg ttaaaggtaa accctgctgg
    57061 agttatccct gttatctttg ccagttcgat tactgcagcg cctgcggcta ttcttcagtt
    57121 tttgagtgcc acaggtcatg attgggcttg ggtaagggta gcacaagaga tgttggcaac
    57181 tacttctcca actggtattg ccatgtatgc tttgttgatt attctcttta cattcttcta
    57241 tacgtttgta cagattaatc ctgaaaaagc agcagagagc ctacaaaaga gtggtgccta
    57301 tatccatgga gttcgtcctg gtaaaggtac agaagaatat atgtctaaac ttcttcgtcg
    57361 tcttgcaact gttggttccc tcttccttgg tgtgatttcc attttaccga ttgcagctaa
    57421 agatgtattt ggtctttctg atgttgttgc ctttggtgga acaagtctct tgatcattat
    57481 ctctacaggt atcgaaggaa tcaagcaatt ggaaggttac ctattgaaac gtaagtatgt
    57541 tggtttcatg gacagaacag aataaaagta tttactgaat cagtaaatgc tgagggagtg
    57601 gaggtttaaa ctctgacatt tgtaagagtt ggatctcccc tcttctattt tgtttttaaa
    57661 taggggtgaa aagacttttt gcttctattt aaaaataaaa taaggagatc aaatcatgaa
    57721 tcttttgatt atgggcttac ctggtgcagg taagggaact caagcagcaa aaatcgtaga
    57781 acaattccat gttgcacata tctcaacagg tgatatgttc cgcgctgcaa tggcaaatca
    57841 aactgaaatg ggtgttcttg ctaagtcata tattgacaag ggtgaattgg ttcctgacga
    57901 agttacaaat ggaatcgtaa aagaacgcct ttcacaagat gatattaaag aaacaggatt
    57961 cttattggat ggttacccac gtacaattga acaagctcat gccttggaca aaacattggc
    58021 tgaacttggc attgaactag aaggtgttat caatattgaa gtgaaccctg acagcctctt
    58081 ggaacgtttg agtggccgta tcatccaccg cgtaactgga gaaactttcc acaaggtctt
    58141 taacccacca gttgactata aagaagaaga ttactaccaa cgtgaagatg ataagcctga
    58201 gacagtaaaa cgtcgtttgg atgttaatat tgctcaagga gaaccaatca ttgctcacta
    58261 ccgtgccaaa ggtttggttc atgacatcga aggtaatcaa gatatcaatg atgtcttctc
    58321 agatattgaa aaagtattga caaatttgaa ataaagcgtt tttcatactt gcaaaaattc
    58381 gctacaaatg ttatactgag atagtctgac ttataattgt tgtctctgtg tctagaggca
    58441 tcgaatcgaa atttatggag gtgcttttgc gtggcaaaag acgatgtgat tgaagttgaa
    58501 ggcaaagtag ttgatacaat gccgaatgca atgtttacgg ttgaacttga aaatggacat
    58561 cagattttag caacagtttc tggtaaaatt cgtaaaaact atattcgtat tttagcggga
    58621 gatcgtgtta ctgtcgaaat gagtccatat gacttgacac gtggacgtat cacttaccgc
    58681 tttaaataat cgaaaaactt ggagggataa gaaatgaaag taagaccatc ggtcaaacca
    58741 atttgcgaat actgtaaagt tattcgtcgt aatggtcgtg ttatggtaat ttgcccagca
    58801 aatccaaaac acaaacaacg tcaaggataa gatagaaagg agaaaacatg gctcgtattg
    58861 ctggagttga tattccaaat gacaaacgcg tagtaatctc attgacttat gtttatggta
    58921 tcggacttgc aacatctaag aaaattttgg ctgctgctgg aatctcagaa gatgttcgtg
    58981 tacgtgatct tacatcagat caagaagatg ctatccgtcg tgaagtggat gcaatcaaag
    59041 ttgaaggtga ccttcgtcgt gaagtaaact tgaacatcaa acgtttgatg gaaatcggtt
    59101 cataccgtgg tatccgtcac cgtcgtggac ttcctgtccg tggacaaaac actaaaaaca
    59161 acgcccgcac tcgtaaaggt aaagctgttg cgattgctgg taagaaaaaa taatatagga
    59221 ggtaaaagtc ttggctaaac caacacgtaa acgtcgtgtg aaaaagaata tcgaatctgg
    59281 tattgctcat attcacgcta catttaataa cactattgtt atgattactg atgtgcatgg
    59341 taatgcaatt gcttggtcat cagctggtgc tcttggtttc aaaggttctc gtaaatctac
    59401 accattcgct gctcaaatgg cttctgaagc tgctgctaaa tctgcacaag aacacggtct
    59461 taaatcagtt gaagttactg taaaaggtcc aggttctggt cgtgagtcag ctattcgtgc
    59521 gcttgctgcc gctggtcttg aagtaacagc aattcgtgat gtgactccag tgccacacaa
    59581 tggtgctcgt cctccaaaac gtcgccgtgt ataatcatcg cattacactg cttttcgttt
    59641 aagagggagt aactaaatga tcgagtttga aaaaccaaat ataacaaaaa ttgatgaaaa
    59701 taaagattat ggcaagtttg taatcgaacc acttgaacgt ggctacggta caactcttgg
    59761 taactctctt cgtcgtgtac ttctagcttc tctaccagga gcagctgtga catctatcaa
    59821 cattgatggt gtgttacatg agtttgacac agttccaggt gttcgtgaag acgtgatgca
    59881 aatcattttg aacattaaag gaattgcagt gaaatcgtac gttgaagacg aaaaaatcat
    59941 cgaactggat gttgaaggtc ctgctgaagt aacagctggt gacattttga cagatagcga
    60001 tattgaaatt gtaaatccag atcattatct ctttacaatt ggtgaaggtt cttctctaaa
    60061 agcgactatg actgttaaca gtggtcgtgg atatgtacct gctgatgaaa ataaaaagga
    60121 taatgcacca gttggaacac ttgctgtaga ttctatttat acaccagtta caaaagtcaa
    60181 ctatcaagtg gaacctgctc gtgtaggtag caatgatggt ttcgacaaat taacccttga
    60241 aatcttgaca aatggaacaa ttattccaga agatgcttta gggctttcag cacgtatttt
    60301 gacagaacat cttgatttgt ttacaaatct tactgagatt gctaagtcaa ctgaagtgat
    60361 gaaagaagct gatactgaat ctgacgaccg tattttagat cgtacgattg aggaactgga
    60421 cttgtctgtg cgttcataca actgtttaaa acgtgccggt atcaatactg tgcatgattt
    60481 gacagaaaaa tctgaagcag agatgatgaa agtacgaaat cttggacgca agagtttgga
    60541 agaagtgaaa ctcaaactca ttgatttggg tcttggatta aaagataaat aaaggaggaa
    60601 tacatggctt accgtaaact aggacgcact agctcacaac gtaaagcaat gcttcgcgat
    60661 ttgacaactg accttttgat caacgaatca atcgtgacaa ctgaagctcg tgctaaagaa
    60721 atccgtaaaa ctgttgaaaa aatgattact ctaggtaaac gtggtgattt gcatgcacgt
    60781 cgtcaagcag ctgctttcgt acgtaatgaa atcgcatctg aaaactatga tgaagcaact
    60841 gataagtaca cttctactac agcacttcaa aaattgttct cagaaatcgc acctcgttat
    60901 gctgaacgta acggtggata cactcgtatc cttaaaactg aaccacgtcg tggtgatgca
    60961 gcgccaatgg cgatcatcga attagtataa aatcatcaat tttgttgagt gttatgatga
    61021 tggagtcttg tgctcttagt ctagctctgg tctaccgcta ggatttcggt cctagcggga
    61081 acactcatca taagttggga tagtagacgc ttgtttacga aattgttttt ttcttaagaa
    61141 caacttcgta agcaggcgtt tttgagtatt ttcgttagaa ttatgctata ctatttgaaa
    61201 agaatcctgt ttaatgttaa ggtttcttat tttaagaaga attggagttt acttatgaaa
    61261 gccattataa ctgttgttgg taaagataaa tctggaattg ttgcaggtgt ttctggtaaa
    61321 attgcagaat taggattgaa tattgacgat atctctcaaa ctgtcttgga tgaatatttt
    61381 acgatgatgg ctgttgtatc tagtgatgaa aagcaagatt ttacctatct tcgtaatgaa
    61441 tttgaagctt ttgggcaaac tttgaatgta aaaatcaata ttcagagtgc agcgattttc
    61501 gaagctatgt ataatatcta ggaggtcatc atggatatta gacaagttac tgaaaccatc
    61561 gccatgattg aggagcaaag cttcgatatt agaaccatta ccatggggat ttctcttttg
    61621 gactgtatcg atccagatat caatcgtgct gcggagaaaa tctatcaaaa aattacgaca
    61681 aaggcggcta atttagtagc tgttggtgat gaaattgcgg ctgagttggg aattcctatc
    61741 gttaataagc gtgtatcggt gacacctatt tctctgattg gggcagcgac agatgcgacg
    61801 gactacgtgg ttctggcaaa agcgcttgat aaggctgcga aagagattgg tgtggacttt
    61861 attggtggtt tttctgcctt agtacaaaaa ggttatcaaa agggagatga gattctcatc
    61921 aattccattc ctcgcgcttt gactgagacg gataaggtct gctcgtcagt caatatcggc
    61981 tcaaccaagt ctggtattaa tatgacggct gtggcagata tgggacgaat tatcaaggaa
    62041 acggcaaatc tttcagatat gggagcggcc aagttggttg tattcgctaa tgctgttgag
    62101 gacaatccat ttatggcggg tgcctttcat ggtgttgggg aagcagatgt tatcatcaat
    62161 gtcggagttt ctggtcctgg tgtggtgaaa cgtgctttgg aaaaagttcg tggacagagc
    62221 tttgatgtag tagccgaaac agttaagaaa actgccttta aaatcactcg tatcggtcaa
    62281 ttggttggtc aaatggccag tgagagactg ggtgtggagt ttggtattgt ggacttgagt
    62341 ttggcaccaa cccctgcggt tggagactct gtggcacgtg tccttgagga aatggggcta
    62401 gaaacagttg gcacgcatgg aacgacagct gccttgcccc tcttgaacga ccaagttaaa
    62461 aagggtggag tgatggcctg taaccaggtc ggtggtctat ctggtgcctt tatccctgtt
    62521 tctgaggatg aaggaatgat tgctgcagtg caaaatggct ctcttaattt agaaaaacta
    62581 gaagctatga cggctatctg ttctgttgga ttggatatga ttgccatccc agaagatacg
    62641 cctgctgaaa ctattgcggc tatgattgcg gatgaagcag caatcggtgt tatcaacatg
    62701 aaaacaacag ctgttcgtat cattcccaaa ggaagagaag gcgatatgat tgagtttggt
    62761 ggtctattag gaactgcacc cgttatgaag gttaatgggg cttcgtctgt cgacttcatc
    62821 tctcgcggtg gacaaatccc agcaccaatt catagtttta aaaattaaga aaataggaga
    62881 aattttaagt tctatttaag attagatgtg tatactataa tcattaaata aagacctcct
    62941 aatattattt gaaacagata acactgaatt agtttgaatt tgattttcat ctaatatctt
    63001 tatttaatga actcctaaac tttttcataa taatctcctt caaaagtcgc ctgtatgggt
    63061 ggcttttatt ttatcattca tgatataata gaagcaaacg gaggacggaa aatggtaaaa
    63121 gtacgattgt atttggtacg tcatggcaag accatgttta acacgattgg tcgcgcgcaa
    63181 ggttggagcg atactccctt aactgctgaa ggtgaacgag ggattcaaga gttaggaatc
    63241 ggtttgcgag aatctgatct acagtttgag cgcgcttatt cgagtgattc tggtcgtacc
    63301 attcagacca tgggaattat ccttgaagaa cttggcttgc aggggaaaat cccttatcgc
    63361 atggacaagc gtatcagaga atggtgtttc ggtagttttg atggagccta tgatggcgat
    63421 cttttcatgg gcattattcc tcgtatcttt aatgtggacc acgttcacca attgtcttat
    63481 gctgaactgg ctgagggctt ggtagaggtc gatacagctg gttgggctga aggctgggaa
    63541 aaactcagtg gccgaatcaa ggaaggcttt gaaatgattg caaaagaaat ggaagatcaa
    63601 ggtggaggta acgcccttgt tgtcagccat ggaatgacta ttggaaccat tgtttatctg
    63661 attagtggca tgcatccgca tggtctagat aatggtagcg tgacgattct tgaatatgag
    63721 gacggtcagt ttagggttga agttgtcggt gaccgtagtt accgagagct aggacgtgag
    63781 aagatggaag aaggctctat ttaatcagtc tagacttgct tgccatgagc tagggatttg
    63841 ataagaatat caagataaga aaaaacagcc gagggtactc ctttcggctg tttttgatgt
    63901 ggaaaactaa agtgtaatgc tattgctttt agagattttc ataaacaaga gcaaggaacc
    63961 tactgttaga acagtcagga tagttgacaa ggctgcggct acaccgtaat ttcctctgag
    64021 aacctctgta taaatagcta cagtcattgt tcttgttttg acattgtaga ggaggataga
    64081 agtagagagt tttgaaatca ttgtgaccca agataagatg gctccagaaa tgataccaga
    64141 tagcatcatt ggagttgtaa tcttagcaaa ggtattgaga cgactacttc ctaagctttc
    64201 agcagcttct tcaatacttg gtgctatttg ttgtaagcta gcaacagatg agcgaatagt
    64261 atacggtaat cttctgacag atagagacat aatcaagatg aaagcagtcc ctgtaatcat
    64321 aagaaatcca cttccaaata gaccagtatt gaaggaagaa atgaaggcaa tccctagaac
    64381 ggttcctggt acaatataag gtaccatact gaggctgtca attaagtttg taaacaaatt
    64441 ccgttttcta acggctaggt aggagataaa tgtcgtaaat agaacaacta gaactaaggc
    64501 aatcaaaggg atacgaatgg tattgaaaat agcagatccc atacgattga aagctacctt
    64561 gtaactgttt ggagaataac ctttaacaaa taccatacct gatgttttta ggaaagaggt
    64621 ataaattaag tagatttgag gtaaaacaga gataaagata attccgtaga ctgttgcata
    64681 aatggcagcc atttttcctt ttgtagtttt tttaggctca attggatgga gcagattcat
    64741 gctgaaactg tagcggtttg caatgtgttt ttggataagg aaaattgcca aggcaatgat
    64801 aatcgccata attgcaaaag cagaatttcc tccaacctcg ctaataaatt gggtataaat
    64861 caggacaggg aaagtccgat atccttcgcc aatcaacata ggcgttccaa agtctgagaa
    64921 tgctctcata aatacaagca aggagctgct agtaaggttg gaactaggag aggtaaaaca
    64981 accgttacga taggtttaaa tccgaaggac cccatgcttt cagctgcttc aagtagagaa
    65041 ttgtcaatac tgttcattgt tccagcaaca tatagaaata ccagtgggaa tagttgcagt
    65101 gtaaagacaa gtataattcc tttgaatcca taaatatcga tagctggaag ataaagggca
    65161 tttgtcaaaa atttagtgat gacctcattt cgtcccagca agagaatcca ggagtaggct
    65221 cctacgaaag gagttgacat ggaagcaatg ataatcaata tttgtagaaa tttcttcccc
    65281 ttgaagtcat acatagagaa gagataagct aatagggttc ctacaactaa ggaagtgaca
    65341 gtagcggtaa tggaaaccct gaaactgttg actagtgtct cagagtagta ggctttacta
    65401 aagaaagtga caaaattagc tagtgagaat tatccttcat gtataagtgc ttacttgagc
    65461 acggtaacga taggataaac gagaaagaca agataggtaa gaaagatgaa gaaagaggag
    65521 gctgtccaaa tatttagttt tttacgttcc atggttgact ccttttatca ggttttggga
    65581 accatctgca gaaaagatgt ttaatttttg cgtattgatt cgtagacgaa tacgattgcc
    65641 tttttgtagt tcttcttcaa aagttgattc ttcactaact tgaatttttg aggcaaaacc
    65701 tgtctcaatg aaatactccg tatttagtcc aagatagacg ctatcgctaa tagttccttc
    65761 aatatctcca gattcatctt tgataaactc ttcgggacga atgcttacat gaatagcttg
    65821 ctcctcaacc tgatcaagag ctagcattcg aagggcatag ccatctgaaa agacgatata
    65881 agcgccgtcg ctccgttttt caagattggc agggataata tttgtgcgtc cgataaaggt
    65941 tgccacaaac tcattagctg gtttatgata gagttctttt ggtcggccga tttgttggat
    66001 caccccatct ttcataacag caatttggtc tgaaatagcc atggcttctt cttggtcgtg
    66061 ggttacataa acagttgtaa ttcccacttc gtgttggatt tctcggatgg cttgacgcat
    66121 atccaagcga agtttggcct ccagattact aagtggctcg tccatgagga gaacacttgg
    66181 attaaccgct aagacgcatg ccaaggtgac acgttgttgt tgtccaccac tgagtttatc
    66241 gggctttcga tccgcatatt gagcaatttg catgagtcca agatacttgt tggtctgttg
    66301 aatcaattct tcttttggaa ccttcttttg cataagacca aaagcaacgt tgtctcggac
    66361 agtcaaatgt gggaaaatag cgtagttttg gaaaaccagc ccgatattgc gtttgctggg
    66421 ttccatatta ttgatttttg tatcatcgaa gtaaaattct ccatctttga tactgttgaa
    66481 acctgcaatc atacgaagaa gggtcgtttt cccacatcct gaagctccaa gaagggtaaa
    66541 gagacttcct tttggaattg taatgttcaa attctcaata acagggacat cgtggtagat
    66601 ttttttggcg ttaataattt cgatctcact catagtgaac ctcttttact gtttagattg
    66661 gatatctgta aagacttcgt tgtatttctt aacgatatct gatttattct tgatgacata
    66721 atcataatct tcagtgagtg ttttgatttt gtcaattggt ttcatgtttt cgcttgtttt
    66781 agcattttta agaacaggac ggttagtagt ggttgtacca agtgtatctt gtacttcttg
    66841 agagataata aaatcgataa atttcttggc attttccata tttttagatt ttttaacgat
    66901 agcagcacta gcaggtagga agacggttcc ttcttttgga tagactacct taatgttagc
    66961 tccgtcattt aagagtttaa ctgctggatc ttcataagag agaccaacag tcatttctcc
    67021 atcagcgact gctttataga caccagatga acttaaaccg attttatagt atattgaaac
    67081 tagaatagta cacatctact tctaaaacat tgttagaaat cgatttgact gtcctgatta
    67141 atttgtcttg ttcttatttc attttactat accatcaata agtgtgaaaa gatcttttac
    67201 ataagaccaa gccttatcat ctttgtaacc accttgagct tgtagcatat ttgttaattg
    67261 agcaaaggcg ctagaagagt ttgctgggtc agcagttgcg atttttcctt ttagttcagg
    67321 tttgagaagg tcgctatatc cttcgatgtt catgccttta gttaaatcag ggtggacgat
    67381 taaaacacta ccatctagtg tataaggagt agagaagcca gttgtgtttt gatattcttt
    67441 gataacatta tcattttctt ttgaaatata gttttcaaag agttctccgt gggtagcata
    67501 ttgtgtataa gaaccaccaa agataacatc agctacagga acttcttttt ctgactctag
    67561 ttttttgaaa agttctccag taccagcttg aatcagttct attttgatac catatttttc
    67621 ttcaaaggca ggaatagttg ctccaattaa gccttctgag tttggtcaat aaacgactag
    67681 cgaaccaccg tctcctttat cagatgaact gtcatcggca gattcattag aagaacaagc
    67741 agcataatac atccatttct ttttcatgat ggatacctcc gttgtgttat ttaagtttat
    67801 tttaaaacaa tgtaagcgtt tttaaaacct acaattct
//
LOCUS       DAVKAO010000003        67346 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKAO010000003 DAVKAO010000000
VERSION     DAVKAO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.003"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(19..315)
                     /locus_tag="VWX16_000173"
     CDS             complement(19..315)
                     /locus_tag="VWX16_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157613.1"
                     /translation="MVKINKICSIQGSSVENEDTVGSQNQYFWIIDGATDLYNSKEEI
                     GYSVSEVVHILSKSLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(299..2425)
                     /gene="pfbA"
                     /locus_tag="VWX16_000174"
     CDS             complement(299..2425)
                     /gene="pfbA"
                     /locus_tag="VWX16_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9157614.1"
                     /translation="MKYFVPNEVFSIRKLRVGTCSVLLAISILGSQGILADEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVINNNSKETEQPNI
                     ELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSSKYISFIKSNANGKE
                     PVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVIDSNQKNGEFNS
                     SKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVKQKLPKTGSNKI
                     MELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3589..4815)
                     /locus_tag="VWX16_000175"
     CDS             complement(3589..4815)
                     /locus_tag="VWX16_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9157615.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLY
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4858..5550)
                     /locus_tag="VWX16_000176"
     CDS             complement(4858..5550)
                     /locus_tag="VWX16_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9157616.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALIL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5723..7474)
                     /locus_tag="VWX16_000177"
     CDS             complement(5723..7474)
                     /locus_tag="VWX16_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157617.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     VNMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIHFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(7464..9209)
                     /locus_tag="VWX16_000178"
     CDS             complement(7464..9209)
                     /locus_tag="VWX16_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157618.1"
                     /translation="MSIIQKFWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9415..9792)
                     /locus_tag="VWX16_000179"
     CDS             complement(9415..9792)
                     /locus_tag="VWX16_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9157619.1"
                     /translation="MKRLIDGKPTFLIKNGNIDPEACRSVGLSASDVALKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYK"
     gene            complement(9864..10320)
                     /locus_tag="VWX16_000180"
                     /pseudo
     CDS             complement(9864..10320)
                     /locus_tag="VWX16_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10454..11281
                     /gene="xth"
                     /locus_tag="VWX16_000181"
     CDS             10454..11281
                     /gene="xth"
                     /locus_tag="VWX16_000181"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9157620.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11293..11436
                     /locus_tag="VWX16_000182"
     CDS             11293..11436
                     /locus_tag="VWX16_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9157621.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            11436..11675
                     /locus_tag="VWX16_000183"
     CDS             11436..11675
                     /locus_tag="VWX16_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9157622.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12059..12154
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12505..13086
                     /locus_tag="VWX16_000184"
     CDS             12505..13086
                     /locus_tag="VWX16_000184"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9157623.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13086..14348
                     /locus_tag="VWX16_000185"
     CDS             13086..14348
                     /locus_tag="VWX16_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9157624.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKIANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14398..14859)
                     /locus_tag="VWX16_000186"
     CDS             complement(14398..14859)
                     /locus_tag="VWX16_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU9157625.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(14856..15620)
                     /locus_tag="VWX16_000187"
     CDS             complement(14856..15620)
                     /locus_tag="VWX16_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9157626.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCDIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(15801..16205)
                     /locus_tag="VWX16_000188"
     CDS             complement(15801..16205)
                     /locus_tag="VWX16_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9157627.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(16275..17756)
                     /locus_tag="VWX16_000189"
     CDS             complement(16275..17756)
                     /locus_tag="VWX16_000189"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9157628.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARTNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKTL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(17775..18953)
                     /locus_tag="VWX16_000190"
     CDS             complement(17775..18953)
                     /locus_tag="VWX16_000190"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9157629.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLERDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYKEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            19118..20125
                     /gene="galR"
                     /locus_tag="VWX16_000191"
     CDS             19118..20125
                     /gene="galR"
                     /locus_tag="VWX16_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9157630.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20171..21208)
                     /locus_tag="VWX16_000192"
     CDS             complement(20171..21208)
                     /locus_tag="VWX16_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9157631.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKVGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(21209..21562)
                     /locus_tag="VWX16_000193"
     CDS             complement(21209..21562)
                     /locus_tag="VWX16_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9157632.1"
                     /translation="MNIKSASDLLEISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQIQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(21806..22696)
                     /locus_tag="VWX16_000194"
     CDS             complement(21806..22696)
                     /locus_tag="VWX16_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9157633.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            22829..23365
                     /locus_tag="VWX16_000195"
     CDS             22829..23365
                     /locus_tag="VWX16_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9157634.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(23532..24338)
                     /locus_tag="VWX16_000196"
     CDS             complement(23532..24338)
                     /locus_tag="VWX16_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9157635.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(24683..26203)
                     /locus_tag="VWX16_000197"
     CDS             complement(24683..26203)
                     /locus_tag="VWX16_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9157636.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITENQMSQLNYQVGVEGKSAEQVAKEFLQEQGLLKK"
     gene            complement(26196..26924)
                     /locus_tag="VWX16_000198"
     CDS             complement(26196..26924)
                     /locus_tag="VWX16_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157637.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(26939..27703)
                     /locus_tag="VWX16_000199"
     CDS             complement(26939..27703)
                     /locus_tag="VWX16_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9157638.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAKYDIFRLKNGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(27707..28150)
                     /locus_tag="VWX16_000200"
     CDS             complement(27707..28150)
                     /locus_tag="VWX16_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9157639.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(28832..29128)
                     /locus_tag="VWX16_000201"
     CDS             complement(28832..29128)
                     /locus_tag="VWX16_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9157640.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(29150..30214)
                     /gene="pepA"
                     /locus_tag="VWX16_000202"
     CDS             complement(29150..30214)
                     /gene="pepA"
                     /locus_tag="VWX16_000202"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9157641.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            30614..31315
                     /gene="galE"
                     /locus_tag="VWX16_000203"
     CDS             30614..31315
                     /gene="galE"
                     /locus_tag="VWX16_000203"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9157642.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKVYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            31738..32697
                     /locus_tag="VWX16_000204"
     CDS             31738..32697
                     /locus_tag="VWX16_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9157643.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            32687..33643
                     /locus_tag="VWX16_000205"
     CDS             32687..33643
                     /locus_tag="VWX16_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9157644.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            33640..34392
                     /locus_tag="VWX16_000206"
     CDS             33640..34392
                     /locus_tag="VWX16_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9157645.1"
                     /translation="MKLENIDKSIQKQDILQGISLKVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            34488..35453
                     /locus_tag="VWX16_000207"
     CDS             34488..35453
                     /locus_tag="VWX16_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9157646.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGVADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(35678..35920)
                     /gene="yidD"
                     /locus_tag="VWX16_000208"
     CDS             complement(35678..35920)
                     /gene="yidD"
                     /locus_tag="VWX16_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9157647.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(35920..36642)
                     /locus_tag="VWX16_000209"
     CDS             complement(35920..36642)
                     /locus_tag="VWX16_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9157648.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(36629..37198)
                     /locus_tag="VWX16_000210"
     CDS             complement(36629..37198)
                     /locus_tag="VWX16_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9157649.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(37217..37945)
                     /locus_tag="VWX16_000211"
     CDS             complement(37217..37945)
                     /locus_tag="VWX16_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9157650.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPIELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(37945..38685)
                     /gene="xerD"
                     /locus_tag="VWX16_000212"
     CDS             complement(37945..38685)
                     /gene="xerD"
                     /locus_tag="VWX16_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9157651.1"
                     /translation="MPMRDRISAFLEEKQSLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQRRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(38676..39137)
                     /locus_tag="VWX16_000213"
     CDS             complement(38676..39137)
                     /locus_tag="VWX16_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9157652.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAKGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(39134..39655)
                     /locus_tag="VWX16_000214"
     CDS             complement(39134..39655)
                     /locus_tag="VWX16_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9157653.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(39631..40602)
                     /locus_tag="VWX16_000215"
     CDS             complement(39631..40602)
                     /locus_tag="VWX16_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9157654.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(40599..41393)
                     /gene="racE"
                     /locus_tag="VWX16_000216"
     CDS             complement(40599..41393)
                     /gene="racE"
                     /locus_tag="VWX16_000216"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9157655.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIGEYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(41600..41848)
                     /locus_tag="VWX16_000217"
     CDS             complement(41600..41848)
                     /locus_tag="VWX16_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9157656.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(42104..43729)
                     /gene="treC"
                     /locus_tag="VWX16_000218"
     CDS             complement(42104..43729)
                     /gene="treC"
                     /locus_tag="VWX16_000218"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9157657.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(43907..45874)
                     /gene="treP"
                     /locus_tag="VWX16_000219"
     CDS             complement(43907..45874)
                     /gene="treP"
                     /locus_tag="VWX16_000219"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9157658.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            46059..46769
                     /gene="treR"
                     /locus_tag="VWX16_000220"
     CDS             46059..46769
                     /gene="treR"
                     /locus_tag="VWX16_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9157659.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            46924..48259
                     /locus_tag="VWX16_000221"
                     /pseudo
     CDS             46924..48259
                     /locus_tag="VWX16_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(48334..49260)
                     /locus_tag="VWX16_000222"
     CDS             complement(48334..49260)
                     /locus_tag="VWX16_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157660.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(49271..50338)
                     /locus_tag="VWX16_000223"
     CDS             complement(49271..50338)
                     /locus_tag="VWX16_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157661.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSKTHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(50347..51273)
                     /locus_tag="VWX16_000224"
     CDS             complement(50347..51273)
                     /locus_tag="VWX16_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9157662.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(51273..52769)
                     /locus_tag="VWX16_000225"
     CDS             complement(51273..52769)
                     /locus_tag="VWX16_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9157663.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(52836..54815)
                     /locus_tag="VWX16_000226"
     CDS             complement(52836..54815)
                     /locus_tag="VWX16_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9157664.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            complement(55501..56694)
                     /locus_tag="VWX16_000227"
     CDS             complement(55501..56694)
                     /locus_tag="VWX16_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9157665.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(56832..58274)
                     /gene="gtfA"
                     /locus_tag="VWX16_000228"
     CDS             complement(56832..58274)
                     /gene="gtfA"
                     /locus_tag="VWX16_000228"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9157666.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVTF"
     gene            complement(58551..58631)
                     /locus_tag="VWX16_000229"
                     /pseudo
     CDS             complement(58551..58631)
                     /locus_tag="VWX16_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(58632..59465)
                     /locus_tag="VWX16_000230"
     CDS             complement(58632..59465)
                     /locus_tag="VWX16_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9157667.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            complement(59479..60345)
                     /locus_tag="VWX16_000231"
     CDS             complement(59479..60345)
                     /locus_tag="VWX16_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9157668.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(60359..61618)
                     /locus_tag="VWX16_000232"
     CDS             complement(60359..61618)
                     /locus_tag="VWX16_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9157669.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANVFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(61653..63815)
                     /locus_tag="VWX16_000233"
     CDS             complement(61653..63815)
                     /locus_tag="VWX16_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9157670.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHTQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     ELQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            63923..64783
                     /locus_tag="VWX16_000234"
     CDS             63923..64783
                     /locus_tag="VWX16_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9157671.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELSQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYIRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            64780..65715
                     /locus_tag="VWX16_000235"
     CDS             64780..65715
                     /locus_tag="VWX16_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9157672.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(65841..65928)
                     /locus_tag="VWX16_000236"
     tRNA            complement(65841..65928)
                     /locus_tag="VWX16_000236"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65892..65894),aa:Ser,seq:gct)
     gene            complement(65935..66008)
                     /locus_tag="VWX16_000237"
     tRNA            complement(65935..66008)
                     /locus_tag="VWX16_000237"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65972..65974),aa:Ile,seq:gat)
     gene            complement(66044..66114)
                     /locus_tag="VWX16_000238"
     tRNA            complement(66044..66114)
                     /locus_tag="VWX16_000238"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66080..66082),aa:Gly,seq:tcc)
     gene            complement(66134..66206)
                     /locus_tag="VWX16_000239"
     tRNA            complement(66134..66206)
                     /locus_tag="VWX16_000239"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66171..66173),aa:Phe,seq:gaa)
     gene            complement(66210..66283)
                     /locus_tag="VWX16_000240"
     tRNA            complement(66210..66283)
                     /locus_tag="VWX16_000240"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66247..66249),aa:Met,seq:cat)
     gene            complement(66297..66386)
                     /locus_tag="VWX16_000241"
     tRNA            complement(66297..66386)
                     /locus_tag="VWX16_000241"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66348..66350),aa:Ser,seq:tga)
     gene            complement(66397..66470)
                     /locus_tag="VWX16_000242"
     tRNA            complement(66397..66470)
                     /locus_tag="VWX16_000242"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66434..66436),aa:Ile,seq:cat)
     gene            complement(66484..66557)
                     /locus_tag="VWX16_000243"
     tRNA            complement(66484..66557)
                     /locus_tag="VWX16_000243"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66521..66523),aa:Met,seq:cat)
     gene            complement(66563..66636)
                     /locus_tag="VWX16_000244"
     tRNA            complement(66563..66636)
                     /locus_tag="VWX16_000244"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66600..66602),aa:Pro,seq:tgg)
     gene            complement(66652..66725)
                     /locus_tag="VWX16_000245"
     tRNA            complement(66652..66725)
                     /locus_tag="VWX16_000245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66689..66691),aa:Arg,seq:acg)
     gene            complement(66735..66820)
                     /locus_tag="VWX16_000246"
     tRNA            complement(66735..66820)
                     /locus_tag="VWX16_000246"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66784..66786),aa:Leu,seq:taa)
     gene            complement(66828..66899)
                     /locus_tag="VWX16_000247"
     tRNA            complement(66828..66899)
                     /locus_tag="VWX16_000247"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66865..66867),aa:Gly,seq:gcc)
     gene            complement(66922..66994)
                     /locus_tag="VWX16_000248"
     tRNA            complement(66922..66994)
                     /locus_tag="VWX16_000248"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66959..66961),aa:Thr,seq:tgt)
     gene            complement(67013..67094)
                     /locus_tag="VWX16_000249"
     tRNA            complement(67013..67094)
                     /locus_tag="VWX16_000249"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67058..67060),aa:Leu,seq:tag)
     gene            complement(67100..67172)
                     /locus_tag="VWX16_000250"
     tRNA            complement(67100..67172)
                     /locus_tag="VWX16_000250"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67137..67139),aa:Lys,seq:ttt)
     gene            complement(67197..67269)
                     /locus_tag="VWX16_000251"
     tRNA            complement(67197..67269)
                     /locus_tag="VWX16_000251"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67234..67236),aa:Asp,seq:gtc)
     gene            complement(67272..67344)
                     /locus_tag="VWX16_000252"
     tRNA            complement(67272..67344)
                     /locus_tag="VWX16_000252"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67309..67311),aa:Val,seq:tac)
BASE COUNT        20154 a        15074 c        12482 g        19636 t
ORIGIN      
        1 gattgatttg tcctattatt atttcatttt gctatactct gttaattcat atgagtttaa
       61 accgatttca tctttaacct cgagtaaagc agtttcaaat atttgtttaa gagtttttga
      121 ttctttacaa ttaaccgaca aactttttga taaaatatgt acaacttctg agactgaata
      181 acctatctcc tctttagaat tatataaatc tgtagctcca tcaataatcc aaaaatactg
      241 attttgtgaa cctacagtat cctcattttc tacggaactt ccttgtatcg aacaaatttt
      301 atttatcttt accataatac ttcaaccctt ttagtgtcaa aagtaaacca attcctgtca
      361 ctgttaaaaa tagttccata atcttgttcg aaccagtctt tggtaatttt tgttttacat
      421 ctactatttc tttagattta ttaatatgat tttcagtttc tctgccatct ccaactattt
      481 tatagtttac ttcttctgtc ttattatctt gtttattgtc gatcttgtca ttcatttgtc
      541 tattatcttt acttgagtta aactctccgt tcttctggtt actatcaatt acattatttg
      601 aattagattg tttttcctct ttgttttttt ctttttcgtt tttatcactt aaattatttg
      661 ttacaatttt gtaaagccca ttctccgtta caatattgaa attaccatcg ctatcacgta
      721 taacaggttc tttcccattt gcattagatt tgatgaatga tatatactta ctggataaat
      781 tataaaattg gttatttaaa acggttattt taccctttga atcctcaata acaattcctt
      841 ctttaccacc gaatatacta ttctctgaga ctactaaatt atcactaact cgtaataatt
      901 caatatttgg ttgttctgtt tccttagaat tattattaat tacatttttt gttacagtaa
      961 tatctgatac ttttcctaaa tattctgcac tatcttttgc aactcgtatc gcttttgttt
     1021 taggatcggc aatattaaat atattttcgg cgataaccac ctgtttattt aaatctaata
     1081 ggtctttagt gtttttatag ctataagcat ttactaaagc tgctccgctt tctcgataat
     1141 gtacactctc tcctttaact ttcttatcaa agcgatttcc tttgattaat acatcagtga
     1201 atcctgtaaa acgtacacct gcatacatca tgttatcaaa atgattattt aaaattttaa
     1261 tattagaggg gttctgtgtc gacaatgttt gatagtgtgt gccaattgct gttactaatt
     1321 ccccagattt atcacttttg ccaaaatagg aattttgaat agtcacattt tcagattttt
     1381 tcccatcatc attcaaggca taaggaaaac cttttctagt taatggttca atctgaatgc
     1441 tctccttact tatgatttgc ccatccttca tcgttttggg taaggcttgc ccaagaaaac
     1501 gagaattatc aactaataca tttttcgaac ctgcaatttg aatagcatgc ccttgataac
     1561 tatccttgaa tgttacattt tttatagtta cgttatttga attcccaata gcaaatgcac
     1621 ctgaagaatt tataagtggt agattttttg ctttagttcc ttcttcattc aaagcaccgt
     1681 tcatatcaat cgtaccacca gaataactaa tatcttctgt tgggccccat tctacttgcg
     1741 caccatcatc cgtaaataaa cctgtcataa aaacaatgga agggtgattc ttaatattta
     1801 taccatttag aattgtagct ttctcattca attctaagtg tgtatgactt tttaaaaaaa
     1861 caatttcttt tactaaataa gttccttcag gaaaatatac atttcctcca cctagccctt
     1921 gagctgcagc atctattgca tcttgaattg cttgtcgatc atcatgaatc ccatcaccta
     1981 ccgcaccata atcttttaca cttaaagcag tatcttcaaa ttcttttttg ataattctgt
     2041 cacttataac atcttcctta taactcttat tttcattagt agatgttact gtagattcaa
     2101 ctgttctgtt gtctaattgt gaattggaac gaatagtacc attagagctg atactattaa
     2161 cacttaacga attatctaaa ccattagtgg ttgaattaga ggcaatcatt tcagcagaac
     2221 gattctgatt aacagttggt gaattatcta aatcattagt aattgcatta gaagactctt
     2281 ttgtagccat cggtgaagaa ctagtaacaa cttcatccgc taaaatacct tggcttccca
     2341 aaattgaaat tgccaatagt accgagcaag tccccaccct taatttacga atactgaata
     2401 cctcattagg aacaaaatat ttcatcagaa cctccaattt ttttactatt ttttaacaat
     2461 tgattattac ttttcttaaa atagatagaa tgacaccgct gtcattatct ttatcatata
     2521 ttttccaaat tgaattgtca aggaataaca caagaaaata ttaaaattta tactcttcgg
     2581 aaatctcttc aaactaggtc agtgccgcct tgccctaagt ctaggttact gacttggtca
     2641 gttctatagt atactgaaac taaaatagta cacctctact tctaaaacat tgttagaaat
     2701 cgatttgact gtcccgatca atttgtccta ttcttatttc attttactat atctacaacc
     2761 tcaaagcagg gttttgagct atctatggcc aacttcctag tttgctcttt agccttccat
     2821 tcaaaagtga tgctatcttt cttagcttga atataaaata tcccatttac tatatttaaa
     2881 tttattgata tgttgtaaat tataaatgaa agaacaaata ccttgtcata caagcattcg
     2941 ccagggatat ttacctgaat atcgctcata tggcggattg aaactaaaac actacaatat
     3001 atttcttaaa tagaccttct ataaaagcta gatttaacct cgataaaaat agatgcagtg
     3061 cttctaaaat actggaatag ggcatatagg taatcgtaga caagtgtttt atagattgtc
     3121 tcgcaggaga tctaatgatg tatagcaagc atcactaaac atatagaata tttgtattat
     3181 tataatatca acatactata aattctatca tgcaaaagaa tgactagaac atgaaagaca
     3241 ggcacaaaac tttgaacaat attctgaaaa caaaaaaaga cgcgagtttc cacaatctca
     3301 ctcatggaaa ctcacgtctt tcaaaaaata gggtgaattg aaaactagaa caagacacta
     3361 gattttctaa atgatgtcta gtaaggattc taaatgctac accaaattgc tcacatctta
     3421 tttcaaacca cgggactccc acaagaaagg aaaatcctat aaaaattctc tctctgagga
     3481 gaaaggagcc tcaactacag gtgtaaacta ttatcaattg aaaacaacag tttttacact
     3541 caattagact ttttaaggac aggatcactt tcgcttgact gtagtttgtc atttttttga
     3601 taaagttagc actttttcag aaactgtttt gaaagtctca atgatatagt ctacttcttc
     3661 atcgcttaat ttagtatgaa gagggagggt aatttcattc tcaaagaagg cataggcctt
     3721 aggatagttc gtcatatcaa atccaagatt cttataggct gtcaagagag gaagcggttt
     3781 gtagtgaaca ttacttgcaa ttcctgcttt agccaattct tggatgatga ggttgcgttc
     3841 ttctaggctt gctccttcta cacgggtgat gtagaggtgg cgtgaagatt cgacagtttc
     3901 agtcttgtgt gccaaaggat ggatgcgaga acctgcaaaa ccactatcat agcggtccac
     3961 aatgtcctta cggcgttgca acaaacttgg atagcggtcc aattgtacca aaccaagtga
     4021 agccatgata tcggtcatgt tgcacttata ggctggtgta acgatatcgt attcccatga
     4081 ccccaattgc atcttggcaa gagcatcctt agtttgcccg tgaagggaaa ggatttggaa
     4141 ttccttgtac atctcttcgt catcaatcac tggattggct ttccaagtcg cacttccacc
     4201 ttctgccgtt gtaaagttct taacggcatg gaatgagaag gaagtaaagt cagcgataga
     4261 accagaaggt tgtcctttat aagtagatcc caaagcgtgg gcactatcag agacaatgac
     4321 aatacggtta aaggcctttt gccacttgct tgaagcggta aagaagtcac gttttttctc
     4381 cacgacttgg aacaaacggt cataatcgca aacaatccct gcgagctcta ctgggataat
     4441 caccttagtt ttctcagtga tagcttgctc aagcaggtca tagtccatct caaacgtatc
     4501 tgcttggata tccaccatga caggggttgc tcccacgtgc gtaatgacac tacatgaagc
     4561 cgtataggtc atggctggaa cgatgacttc atcaccaggt cccacttcca aaacgcgtaa
     4621 aatcaactcc agagcagctg tcgcagagtt gagacaaaca gtcttaggtg tctgtgtgta
     4681 aagatacaag cggcgctcca gttcttttgt tttaggacct gttgtgatcc aaccagaacg
     4741 cagggtatcc gctacttcag caatttctgc ttctgtgata tcaggcggtg aaaatggaat
     4801 attgtaattt ggcattgatt tgctcctttt atctgtactc attttcttat gactacttta
     4861 ttttagtacc tcaaacacgg tttgaaacat gattttgatg tccccaaaga aactaaactc
     4921 tcggagatag gcgaggttat agcgcatctt ttcaggaaga acatgctcca cataggcctg
     4981 atcaactgac agacctttct ccgtcatttg actgatgatg gtgtcctcat ccttgtagtt
     5041 gatgctggct ggagaggtaa ttcctgcttg caagagcaag gttgccatca tttcagggct
     5101 atactgctct gtataacgtg gcacttcagg tcgtgtaccg acaaaggaca tctcaccttt
     5161 aaggacattg accaactgag gcagttcgtc caaacggaca cgtcggatga aatttccaac
     5221 cttggtaata cggctatcgt tagcagaagt caccagactt ccttttttat ccgcatccgt
     5281 caccatggta cgaaacttcc aaatcttgaa ccgacggttg tactgggtca cacgctcttg
     5341 cttgtaaatc actggcccct tgctatccaa cttgatccaa atgctcaaga tgagaaagat
     5401 gggagaggtc agaaccagta agatcaaggc caaaacccag tccaagcaac gcttgaaaat
     5461 cagcgaaccc ttccttttag agacaagctg gtagtaagac tcaacctcgc ttgatttcat
     5521 ttccacaggc aagtcttccc atttcagcat gctgttctcc tttgttttta ttatactatt
     5581 tgtcaacatt tttcattata ccacaaaatg gaaaaggcgg tgaaagaaat ctgtaatttg
     5641 aaggatttcc ttctagtact caatgaaaat caaagtacag ctttgagatt gcagataaag
     5701 ctgacatggt ttaaagagat tttcaaagag tatcggccat ggcccctgcc tgcaaactat
     5761 acatcttgtg ataagttcct cccagagcca agagttcctc atgggttcca ctctcgataa
     5821 tgcgtccctt atccaagaca tagatgcagt tggcgtcttg aatagtagaa aggcggtgag
     5881 cgatagcaat agttgttcgg ccctgtctca tcttcgccag agaagcttga accaaacttt
     5941 ctgtttcaga gtcaatattg gctgtcgctt catccaaaat caggatttta ggctggctgg
     6001 cgactgttct agcaaaggca agaagctggc gctgcccagt agagaagctc gaaccacgct
     6061 cggaaacagg ggagtcgtac ccctgaggaa gttcttgaat aaaggaatct gcatccacaa
     6121 aggctgccgc agcctgaacc tgctcatcac tgatttcttg gtacatggcg atattggact
     6181 taatagttcc atgatagagg aagggttcct gcaagaccaa accgatgttt tttctcagct
     6241 cttcttgact gaaatccctg atatccacat catccaagag aactctccct gactggaatt
     6301 cataaaagcg catgaggaca ttgataatag acgatttccc tgaacctgta tgacctacaa
     6361 aggcaatggt ttcaccctta ttaacagaga aagaaatgtc atccagaatc ggatgtttac
     6421 cgtcatatga gaaacacaca tgttcaaaat ggatattgcc ttcttggact ttagcctgcc
     6481 cattttcttg aaggggctca taggtcctct cgtctatcag ggcaaagaca cgacctgcag
     6541 aaaccatagc cgtttgcaga gttgaaaagt tttgcgtcac ctcaatcaag gggtcaaaaa
     6601 ggcggttgat gtactggata aaggcataca tggtcccgac cgttatcccg atagaaaaac
     6661 cacggtagcc aaagtaggcc atcaagactg catagcctag aagtttcagc aaactcatgg
     6721 caggtctcaa aaagagggca tccaaggcta cagaacggtt ggcgtagacc aagtgttctt
     6781 ggttgatttc atcaaattct gcctgcaggc gcttctcttg attaaaggcc tgaataatcc
     6841 tgattccctc gatattctct gccagcttac tattgatatc tgacaagaga cttctggttt
     6901 tctcgatgat tttcactgac ttttttcgat agagattgac caaaaggaaa atcaaaggaa
     6961 gaaagagcaa gactaaagcc gtcaaacgaa aatccagcac caacatggta taaagggttg
     7021 tcagaaagat aaaaactgct gagataaagc tggataaaat cccagaaaac atatcactaa
     7081 tcgtctcggt atcgttggtc aaacgagaaa cgatagaacc tgctggcgtc ttgtcaaagt
     7141 aagacatgcc cagtttctcc atattgacaa aggcatctcg acgaatatcc ctaacaatac
     7201 tgtaagacac gcgcgcaaag agaagattgc cgacatactg aactacagtt tgtaggatgt
     7261 agagaccata gtagaccagc aaaacggtaa cggctagttg gttaagattg ctgagatact
     7321 ggtcgataaa gtgggaagcc acgaggggta tgacactttt aatgaccgtc gtcgctagga
     7381 gaaaactgag tgccaaaaag gtcaggagtc cataaggctt gagataagac atcaagcgct
     7441 tcaatacagc ccattgttct tgtttattct gcatcttctt ctcctttcat ttccaactgc
     7501 tgagactggt aggtttgggc ataccagcca tccaaagcta gcaagtcttc gtgcctgcct
     7561 cgttcgataa tttgaccatt ttgcaggact aaaatcaaat ctgcatggac aacagcactg
     7621 aggcgatggg cagtgataat ggttgtcttg tcctttcgcg tctccttgag gttgtcgata
     7681 atcgcatact ctgtcttggc atctacggcg gataaggaat catccaaaat caagatatca
     7741 gggtctaaaa tcatagcccg actcatagcc aaccgttgct tttgaccacc agaaagactg
     7801 actccttttt caccaatcag cgtatcaaat ccttgaggca tgtctacaat atcttggtaa
     7861 actcgggcta gcttagtagc ttcctcgacc gctgaaaggg gcaagttagg attgccaaag
     7921 cggatattgt ctaggattga agtcgcaaaa agaaactggt cctgaggaac atagcccatg
     7981 agactgcgaa ggtctgtcag acgatagtcc cgaatatcgt gaccgtttag ataaatggca
     8041 cccttatcca catcgtattc acgcaagagg agcttgatta aggacgtttt cccagagcct
     8101 gtctgcccaa ccaagcccag tgtttgccct tttgccaaac taaagtgaat atccgtcagt
     8161 gtttcctcat tttcaaaagc aaagctgtca atggcatact ccaaacgccc attttcaata
     8221 ccatccagag gaaactcagg gtcttgtaca ggagattcct gagacaaaag attttcaatc
     8281 cgctggtaag aaaccttccc tcgctgagta gtattaaaga ggaagccgat ggccataaga
     8341 ggccagacca gcatatccaa atagctgata aaggtgacta gattcccaac tgtaatctgc
     8401 ccttcctgaa ccatcaagga gccaaccaaa agcgttaaaa cataggacga accaacaaac
     8461 aagagaacca tagggtcaaa gagactatca tatttcatgg tttgcaggtt cttttggaag
     8521 gttaattcat tgactgcctg aaaagacttc aactcgtctg cctgataacc gaaagacttg
     8581 gttactttga tacctgatac ggactcctgt accttgttat tgagttcaga aaaagcagct
     8641 tgggattcgc caaaggcctt atgagtcttt ctccctaggc gactagtcgt ataggccatg
     8701 aaaggtaggg ggagaatggc aacaagagtc atctgccatg agatgctaaa gagcatggtc
     8761 aacaaagtca ccagagccgt gatagaggca tccaccgcag acatgacacc gccacctgct
     8821 aaacgagtca aggaattgat atcattggtt gcgtgtgcca tcagatcacc cgtccgatag
     8881 gtttgataaa aggctgacga catttttgtg aaatgcttaa acaagcgaga ccgcatgatc
     8941 tgtcccaagc aataagaggt cccaaggata tacatacgcc acacatagcg caaatagtac
     9001 ataccaaagg ctgcaagtag caagtaaaat aggctaagaa ggaggtcctg ctgggttaat
     9061 tgccccgatg tgatggcatc aataacccgc cccataacca taggaggaat gagattgagg
     9121 acggaaacca agaccagggc cacaatcccg actagataac ggcgtttttc taacttgaaa
     9181 aaccaccaaa atttttgaat aatggacata aaatcccttt ctgattgcaa atagaaacct
     9241 gaggccaata ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggctgctcaa
     9301 agcactgctt tgaggttgta gatagaactg acgaagtcag taacctacat acggcaaggc
     9361 gacgttgacg cggtttgaag agattttcga agagtacaag accccaggtt tttcttattt
     9421 ataagttacg actgtaacag cacccttgtc atattcagca ataaagatat tggctacatt
     9481 gtcatgccct tgtttactga ggttatcaag caaccactct tcgctacgac caatcgattc
     9541 caagacatct acttgaatca caccgtcagt cacaactgga tacttaggat tttcatctcc
     9601 catttgcaca acgatgagtt gcccattttg ctcttgcaca gctcgtttga cttgcttcat
     9661 ctggaaaatc ccttggctac gaagtttgag ggctacatcc gatgcagaca aaccaactga
     9721 acgacaggct tctgggtcaa tattcccatt tttgataagg aaagttggct taccatcaat
     9781 caaacgtttc acaaagtgaa cattgttgtt gagctatttg agagtcaaga ccagtttgat
     9841 taaaatttcg atataattaa gtgtcatttg ttcacatcca ccagttcaat tgcatctgtc
     9901 ttatacaatt cgactttctc taacaggtac ttgtctggct cacttccgtt tagggcacga
     9961 taatagcgat ctcccacctt gataagtgcg ccatctgtgt ttgtggaagt attaacatag
    10021 acttctgact tgtctactcc caaatctttg gaaacaacct cgatgaaatg aagtgaagtt
    10081 tgaaatttat tgttagaaac ttgattgttt tgataagtcg tgatgctgac caaaagcaga
    10141 gctaaataag gccagagctg aaatcatgac caactcacga aatttggttc cttttttatc
    10201 atggtatgcc ttgaaagcaa aaaaaactgt cacagctaat agaacaatcc caaagccaac
    10261 catgatacca ttttgctgac cgatttggct gagtacatag tcataagagt agaatttcat
    10321 gtattttcac tccctttcat aaaatcattc ttaattataa acgaaagaga gtaatttttc
    10381 aaaccatacg ttggggaaat agatcttttt ttggtataat aaaatctata atctgaatga
    10441 aaaaggtaac tttatgaaac ttatctcatg gaatattgat tccctaaacg ctgccctaac
    10501 tagtgactca gctcgtgcca aattgtccca agaagtccta caaaccttgg tcgctgaaaa
    10561 tgctgatatc attgctatcc aagaaaccaa gctttctgcc aaaggaccta caaagaaaca
    10621 cgtggaaatt ttagaagaac tcttcccagg ctacgaaaac acgtggcgct cttcccaaga
    10681 gcctgcccgt aaaggctatg ctggaaccat gttcctttat aagaaagaac ttacacccac
    10741 tgtcactttc ccagaaatcg gtgccccttc taccatggac ttggaaggcc gcatcatcac
    10801 tctagaattt gatgcatttt tcgtgaccca agtttacaca ccaaatgctg gcgatggtct
    10861 taaacgcttg gaagaacgcc aagtctggga tgtcaaatat gcggagtatt tggctgaact
    10921 agacaaagaa aaaccagtcc ttgcgaccgg tgactacaac gtagcccaca atgaaatcga
    10981 ccttgcaaat cctgctagca accgccgttc acctggattt actgacgagg aacgtgctgg
    11041 atttaccaac ctattggcaa ctggatttac tgataccttc cgccatgttc atggcgatgt
    11101 tcctgaacgc tacacttggt gggcacaacg cagcaaaact tctaaaatca acaatacagg
    11161 ctggagaatc gactactggc tcacaagtaa ccgtatcgct gacaaggtca ctaaatctga
    11221 tatgattgac tcaggtgctc gccaagacca tacaccgatt gtcttggaaa ttgacttgta
    11281 aggattttct atatggacta caatgcggtc attcccgagt ttcttgtatc gaatatcgaa
    11341 cagtcccggt ccttctactg tggtttactg ggttttagga ttgaatacca acgtccagaa
    11401 gaaaactttc tctttctctt gaagagtgtc aactaatgtt agaggaggga acaaaggatc
    11461 aactagctga actgacctac ccctttggtc gtggagtcaa tctctccttt ggcataaagg
    11521 atgtttcaaa actctatcaa aaagtgatgg aggcaaacta tcctatttat cgtcccttga
    11581 ccaaaagaaa gtttcgtgtc agtgatccct atatctatcc tcataaattt gcagttttgg
    11641 atccagatgg ctatttttta agatttagcg agtagaataa taaaaattgg gaaaagtctt
    11701 taaagttaga aaaaggcata ttgtcaggtg tctaaaaact tgatagtatg ccttttatga
    11761 tggaaaaatc ttctaatact cttcgaaaat ctcttcaagc cacgtcaacg tcgccttacc
    11821 gtaggtatat gatactgact ctgtcaattc tatccacaac ctcaaaacag tgttttgagg
    11881 ttgtggatag cttcctagtt tgctctttga ttttcattta gtataaatgt tagtttacaa
    11941 tggagttttt gttcaccctc tcttctgttt gttcgttttt taaaccaaaa cactaacaaa
    12001 aatagttgat ttctaaagtt atttagtgta aaataaaaac attggctcaa gcctatttaa
    12061 aattgaatat cgttttactt gtttatgtcg tgaattggca cgacgtttct acaaggtgcc
    12121 ggaacaccta acaataagta agtcagcagt gagatatggc cgtttttgcc atgtctattt
    12181 aggggactta cttagagatt aagtaggtct tttttgaata tttttaatat ttcccttaat
    12241 tagtgcggac gggaggtgaa attatcctgt gaataatttc aggaatccag ggaatttcat
    12301 ccctaatttt tgagccaaaa gtctcaaaaa tcccgtcata gacaataaca agattattgt
    12361 ctatgatacc actacaacgg gaaatatttg ataagactcg cttcgctcgt aatagtttta
    12421 tcaaatatat agtattagca aaagggatca ctatagaaat atttaaacaa caagtaaatt
    12481 aacttttaga ctttaggagg tcttatgaaa ttattagaag agcgcatcct caaggatggg
    12541 catatcttgg gtgataacat cctcaaggta gattcctttt taacccacca agttgacttt
    12601 agcttgatgc gagagattgg taaggttttt gcggaaaaat ttgctgctac tggcattacc
    12661 aaggtcgtaa ccattgaagc gtcgggtatt gccccagccg tttttacagc tgaagcctta
    12721 aacgttccca tgattttcgc caaaaaagct aagaacatca ccatgaacga aggcatctta
    12781 actgctcaag tctactcctt taccaagcag gtgaccagca ctgtttctat cgctggaaaa
    12841 ttcctctcac cagaggacaa ggttttgatt atcgacgatt tccttgctaa tggccaagct
    12901 gctaaaggct tgattcaaat catcgaacag gccggtgcca cagtccaagc tatcggtatc
    12961 gtgattgaga aatccttcca agatggtcgt gatttgcttg aaaaagcagg ctaccctgtc
    13021 ctatcacttg ctcgcttgga tcgttttgaa aatggtcagg tcgtatttaa ggaggcagat
    13081 ctctaatgca aactcaagaa aaacactcgc aagcagccgt tctgggcttg cagcacttac
    13141 tagccatgta ctcaggatct atcctggttc ccatcatgat tgcgacagcc cttggctatt
    13201 cagctgagca gttgacctac ctgatttcta cagatatctt catgtgtggg gtggcaacct
    13261 tcctccaact ccaactcaac aaatactttg ggattggact cccagtcgtt cttggagttg
    13321 cattccagtc ggtcgctccc ttgattatga ttgggcaaag ccatggtagt ggcgctatgt
    13381 ttggtgccct tatcgcatct gggatttacg tggttcttgt ttcaggcatc ttctcaaaaa
    13441 tagccaatct cttcccatct atcgtaacag gatctgttat taccacgatt ggtttaacct
    13501 tgatccctgt cgctattgga aatatgggaa ataacgttcc agagccaact ggtcaaagtc
    13561 tcttgcttgc agctattact gttctgatta tcctcttgat caacatcttt accaaaggat
    13621 ttatcaagtc tatctctatt ttgattggtc tggttgttgg aactgccatt gctgctacta
    13681 tgggcttggt ggacttctct cctgttgcgg tagctccact tgtccatgtc ccaattccac
    13741 tctactttgg gatgccaacc tttgaaatct catctattgt catgatgtgt attatcgcaa
    13801 cggtgtctat ggttgagtca actggtgttt atctagcctt gtctgatatc acaaaagatc
    13861 caatcgacag cacgcgcctt cgcaacggtt accgcgcaga aggtttggcc gtacttctcg
    13921 gaggaatctt taacaccttc ccttacaccg gattttcaca aaacgttggt ttggttaaat
    13981 tgtcaggcat caaaaaacgc ctgccaatct actacgcagc tggtttcctg gttctccttg
    14041 gactgcttcc taagtttggc gcccttgccc aaatcattcc aagctccgtc ctcggtggtg
    14101 ccatgctggt aatgtttggt tttgtatcaa ttcaagggat gcaaatcctc gcccgtgttg
    14161 actttgctaa caatgaacac aacttcctta tcgcagctgt ttcaatcgct gcaggtgtcg
    14221 gtctcaacaa cagtaatctc tttgtcagca tgccgacagc cttccaaatg ttcttctcaa
    14281 acggaatcgt cgtagccagc ctactcgcta ttgtcctcaa tgccgtatta aatcataaaa
    14341 agaaataaga aaaagaggtg cgagcctctt ttttgttgac aaaataaaaa catctaccta
    14401 ttcatatatg ctagtgtcat agtcctgaaa atcatctgca tcaagaacaa tttcacaact
    14461 cttatacttt tctcttacaa gtcgttcggc ttcatctgga ttttcagcct ctatacttac
    14521 taaacgtgat aaagtttctg taatttctac tgtatacttt ttcagtttta gattgctttc
    14581 caataattca cctattaaca ataatctatg ataggagcta tgtagccact ctggcaattt
    14641 atctcctact tcccacccat atttttcctt catcaaagct agttgttttt cgtcaccaaa
    14701 tttcatattt tcagataacc atctcacaca atcgggatct gataaggtac agacgattaa
    14761 acttaggtta tcgtaacggc tttcagtttc ataatcaggc cagatgactt ttaagaaatc
    14821 atacacctct tctgatgcaa caagttgttt cgttttcata atttccgata ctttcctcta
    14881 cctttaaatt ctattatttc tttgtctctt aatatttgaa gctgttgcct aatcttttct
    14941 ttgatatgat tgttcttaac aaagatattt tttaggtcac tttcaaaacg atacatatct
    15001 tcaagggtaa attctgaacc ctctatctta tctatacaat ttagaatttc tattgtccaa
    15061 cctcttgatg acagagagct cttccttaaa aataaacctt gcttaaattc ttttgtaact
    15121 ttttctggat ctctaacttg tccatcttgc acaagaaata tccttccttt agaaggtact
    15181 tgtgataaat caatgtcaca accaatccaa cctgctcgtc tagcagttgg tgcaagtggt
    15241 tttctttgaa taatcgattt cggtgtaaca aattgcttcg gaaggacaag aaagttattt
    15301 acctcaaaat tttttgtgta agttaaaaaa aagaaattag gattattatc tgcctgcaca
    15361 cgcttcatca tcgttgcata agcaccatca ttgattgttg atgaaaaatt tccttttttg
    15421 ctctttagtt caaactcctc actacaatga ttacagtaaa aatctgctac aggccgatta
    15481 ttttcaaaat gatttaaggg attgttccca caattagggc aataagactg acgataaacc
    15541 caatcctccg ttaagatacg tgccttttgg ctattacttt tataggtttc tactaattct
    15601 aaattaaaat gtaattccat ttaaaaattc ctcactaata tgagaactca ctcgatataa
    15661 gcaattaaaa tctactataa ctagtatacc acatatatgt acctattaca tttgtatttc
    15721 atcactctct tttatatttc cttctaacat atctcataaa ttaaaaatga gtgagataaa
    15781 tttatcccac tcacctttct ttatatactt acccgtcttt cctcagacct ttggcctgtg
    15841 acaaacatgg taaaggttgc tttacagaca tttctgccct cttgattggt gatatccaca
    15901 tccatcacac aggttgtacg accttgatgg acacattctc ctttaatggt caatacatcg
    15961 tcgagttttc ctgccttaag gtagttgata gaggattgga gtgtcacccc atcaagtccc
    16021 agcgagataa ccaccaaacc actgatttgg tcacatagcg tgaatagata gccaccatgg
    16081 gcattgccat agtagttgag cgacgagttc actactttgg tcgtcaccac aacgtgacca
    16141 tctctcattt tttcaatttc gtaattttca aaggcagata tagcgtcaaa atgaaagtct
    16201 ttcatcgttt cctcctgata tttcaaacga cactatgata ctacttttta taggactgtg
    16261 caaggagaaa gctcctagtc tagtaaaatt ccgacctgtt ccacaaagcg cataaaggct
    16321 gtctgccctt gttctgtctg cttgtagact cctgcatcct caagtacacg cacaaagata
    16381 gcacccacag agtccttgac gattgcaagg gttttttctt tatccgttag gtctggatgt
    16441 tgggatttta gttggtctgc ccactcctga tgataatcgg caactgtaac agcttctcct
    16501 acaagatagc tagcgacttg ctccacttct tctttcagac gtggtggcaa gattgccaag
    16561 cccatgacct caatcaagcc gatattttcc ttcttgatat gttggacatc cttgtgggga
    16621 tgatagatgc catcaggatg ctctgctgaa gtctgattgt ctcgcaagac caagtccaac
    16681 tcaaactgtc catcacgctt acgcgctatt ggtgtgatgg tgtgatgcgg tgtcccgtct
    16741 gtctctgcca aaatctgcac actagaatct gaatactgac gccattcctg gaaaatctta
    16801 tcagctaagt taatcaaatc ctctttggaa tccgaagtca agcgtaggac agacattggc
    16861 cacttgataa ttccagcctt gacctgctca aaaccagcaa atcggaaggt cttttgcaag
    16921 ggagccaatt ccataggaaa tacgtgacgg cctccctgat aatgatcatg agttagaata
    16981 gagcccccca caatcggcag gtcggcatta gatccagcaa aatatcctgg aaactgctct
    17041 acgatagcca acagacgttc aaaactctga cgactaatgg ccatgggacg atgctggcca
    17101 tctaaaaaga tacaatgctc attaaagtaa gcatagggcg aatactggaa accccattcc
    17161 tgaccaacca tttcaaaacg gataatccgg tggttggtgc gagctgggtg gttgacccga
    17221 ccatggtaac cctcattctc taaacaaagt tgacactgag gataattact agcttgcacc
    17281 aacttggctg ccgcaatctc tttcggatcc ttttcaggct tagagagatt gatggtaatt
    17341 tcaagttctc cataatcaga tggaacacga taagcgatat ttttagcaat ggccttgagt
    17401 ttgatgtagt catttttctg actgagttga taaaaatcct ctatcgcttg ctcaggagag
    17461 tggacatagg cttcccagaa gtcacgattg acctgactcg gacaaggagt caccaagtcc
    17521 atcaattcag taccaaggat ttcacgcgca gtctgactat cctcaatcgt ctctaatcga
    17581 acggcttcct caactagctg gtccttgagg tcaatcaatt tatccagatt ggtctcaact
    17641 tccaaaacac cttctcccac tcgtgccaaa acacgattgg tcaggtagat tcgatccatt
    17701 tcctcaaatg aactttcaga aatgacatgt gttacaaatt tatctactaa ggtcactata
    17761 gagcctcctt ttgactagtc aaggacgcga gtgccacctg caacttcagc gatatagaag
    17821 cttggagcgt atccaactac ttccttgtag tgtttgccta cagcttcctt aaaggcctca
    17881 acagtatctt tttgaaccaa ggcaatggca cagccaccaa aaccagcccc tgtcatacga
    17941 gcaccgagaa ctccttcttg tgcccaagct gtgtgaacaa gagtatccaa ttccaaacca
    18001 gttacttcat aatcacgctc cagagaaacg tgtgacgcat tcatcaaacg accaaatgtt
    18061 tccaaatcac ctgcttgaag ggctgcttga gctttaaggg tacgttggtt ttcaagcaca
    18121 gcatggcgag cacgtttcaa acgattttca tctttaatca gatagctata ttggtcaacg
    18181 gcccactcgt ccaattcacc cagggtctga atatccaagg caacttgcaa ttcttccact
    18241 gctttttcac actcagcacg acgttcattg tatttagagt ccgccaattc acggcgtttg
    18301 ttggtgttca tgataacaac gacattgtcc ttcaaatcaa gtggcaccaa gtcgtattct
    18361 aaagtattgg tatctaagta aatagcacgt tggtcagccc ccataccaat agcaaattgg
    18421 tccataatgc cagaatttac tccgataaag ttattttctg tttgttttcc gattttaacc
    18481 aaatcgagac gctctagttt taaatcaaag agatgctctg ccacaactcc tgtcaagagc
    18541 tccaaggatg ctgaagaaga caaaccagca ccatttggga tattcccata aacataaaaa
    18601 tcaaaacctt tgtcaatcac gtgcccagct tcttgcaaga aatgaaggac accttttgga
    18661 taattggtcc agttgtgctc tttttcaaac ttgaggtcag cgagaggcac ttcgataatg
    18721 cccttgtcct caaagttagc tgagtagaaa cgcaagactt ggtcgtcacg cttacgagct
    18781 gcaccgtaag ttcccaagga aatagcagca ggaaaaacgt gcccaccgtt gtagtctgta
    18841 tgttcaccaa tcaaattaat gcggcctggt gaaaagaagg tttggtctgc ttcttgacca
    18901 aaaacagcaa gaaagtcttt gcgaagggct tcagtagtaa gatgttgtgc catatgaatt
    18961 ctcctttgac tgtccgttaa gccatcttaa cgtgtaatcg ttttctttct attgtatcca
    19021 aggaattttc caaggtcaat cctttttact aaaattttag taaaatatca gtaaaaagaa
    19081 gaaaaacatg atatactaac ctaatcaagg aggatttatg gctaccttaa aagacattgc
    19141 acagctagcc tctgtctcta tcgcgaccgt atcccgcgtc ctcaatcgcg accagagcct
    19201 atctgttaca gaagaaacca gacaccgtat tttaaccgtt gctgaagagc tgggctacac
    19261 caagcacctc aagacaggcg agtcccacaa acccaagcaa aagattgcca ttatccaatg
    19321 ggtcagcgaa caaggggagc tggacgacct ctactactac cagattcgcc taggaataga
    19381 aaaaagagcc caagagttgg actatgatat cttgcgctat tttaatgacc acccttttac
    19441 cctaagcgag gaagtgattg ggattctctg catcggaaag tttagtcgag ctcagatttc
    19501 tgcctttgaa gaataccaaa agcctcttgt atttctagac agcgatacac tttccctggg
    19561 acatacctgt attatcacgg atttttacac tgctatgaaa caggttgtcg attatttcct
    19621 cagtcaagga atggaccgta tcgggattct aacaggcctt gaagaaacaa cagaccaaga
    19681 agaaatcatt caggacaagc gtctagaaaa cttcaaaaac tacagtcaag cgaggggaat
    19741 ctatcatgat gaactggtct ttcaaggaag atttactgcc cagtctggct atgacttaat
    19801 gaaggaggcc attcagagct tgggagacca acttccgcca gcatttttcg cagccagcga
    19861 tagtttagct atcggtgccc tccgtgccct ccaagaagct ggaatcagcc tgccagatcg
    19921 cgtcagcctc atttccttta acgacactag tctgaccaaa caggtctatc ctcccctctc
    19981 tagtattaca gtttatactg aagaaatggg ccgagcaggt atggatattc ttaacaagga
    20041 agtcctccac ggtcggaaaa tccctagcct gaccatgctg ggaaccagac tgacattaag
    20101 agaaagtacc ctaaatcaag aataggataa cataaaaaac gaatagagtt ctaaaactcc
    20161 tattcgtttt ttattcgatt acaatcatag acttaatggt cttacgttca tccatatctt
    20221 tgtaggcttg gtcgatatct tccagtttat aacttgaagt aaagacgcga cctggattga
    20281 tatcaccatc aaggacggct tttagtaaaa attgcttatc gtatgttgta gcagaagctg
    20341 ccccacctgc tacagagata ttttgcataa atgtcgaacc aagagcacga ttattatagt
    20401 gtgggactcc tacaaagccc atacgccctc cattatgaag aacacctagc gcctgttcta
    20461 tagcagcctc cgtaccaaca cattcaagtg ctgcgtctgc tcctccgccg aggatttcac
    20521 gcaccttggt aattccttct tgaccacgtt ctgcaacaac agctgtcgca cctgactcca
    20581 tagccatctt ttgacggtct tcatgacggc tcataaggat aatttgtgat gctccacgca
    20641 tcttagccgc gatgacagca cattgaccaa cagccccatc accgataaca acaaccttgt
    20701 cccctttttg aacatttgca acacgcgccg catgatagcc tgtcggcatg acatctgcaa
    20761 gagtcaaaag ggacttgagc atcccttctg tatagtcaga aggttgacca gggattttaa
    20821 ccagcgccca gtttgcatag tggaagcgaa tatattctgc ctgaaaatca ccccccaaat
    20881 tattgccaat atgattgtcg caagaaccgt caaatccagc aagacaggca tcacactcac
    20941 cacatccatg tgtaaaaggg acaatcacaa aatcacctgg tttcaccgtc gtaatggctt
    21001 ccccagcttc ttcaacaatc ccaatcgctt cgtgtccact atttttgtgt ccaactttcg
    21061 tttctggatt acggtacctc cataaatctg aaccacaaac gcacgcacga accacacgaa
    21121 taatcacatc atccgcttct attatttgcg gacgttcaat gctagcaagt ccaacctgac
    21181 ctgcctttgt atatactgct gatttcattt aaaattttcc ttccttataa agtttaattt
    21241 tgagatttaa acgatttaaa gctgtctgta tctgagacaa gcgctcctct aatttttgct
    21301 tttcctcttc taaaatacca agcctctcct ctctcgtttc atctcccttt tggtagagcg
    21361 acatatagtc aactaaacta tctacagaga cacccgccga acgaaaacac ttaataaatt
    21421 ccagcgcttc gatatcctga tcttggaaat cacgaatccc agtagcagta cgagtaatcg
    21481 gtggcacaag accaacccgt tcataatacc gaatcgtatc cgctgaaatt tccaacaaat
    21541 cactggcaga tttaatattc acacgctaac ctcctctcat ttcattcact gagttcacgc
    21601 tattctaact cacttatctt attatataac tttgagttga ctccaagtca agcattttgt
    21661 atctaatcga aaaattttta tcattaacaa tcatatatct ggagtttata caatgctctt
    21721 ctcttaccaa tttttgaaat atttaaataa taaagaaatt actctattcc aaataagata
    21781 gtacccaagt atttttcact tttttctaat gttgatgctc ataactccgt tccaagtcac
    21841 acacctttcg cttatgggtt tgatgagttt ctaggtcagc atcaatttca atggtaatat
    21901 tttgaaaacc acaatctttt aggaaaattc gaatagactc tttacaagtt tccatatgtt
    21961 ccatttcttt tagacaaaca tggacaatgg catttttttc caaagcatcc atagtccaga
    22021 gattaagctg attaaggctg gccacattgt ccaatcgttc caggccactc tttacttgct
    22081 tgatatcaag accttctggc acagcatcca agaaaatctt gagtgtagac caaaaacgtg
    22141 aaagggcttt tgaaagaata aagaaagaaa tgacaaggga caaaagagga tctaggatat
    22201 accagtccgt aaatcgaaga acaatcgcca tcaggataac agctacccac cctagcgtat
    22261 cttccagaaa atgcagactc agaatagact cattctttgt ctttccctta ccaaccacca
    22321 gactcgctaa cagattgata gtaatcgcaa taattcctaa ccagagaatc ccctcatcat
    22381 tgactggttg cggatgcaaa atcttcgtga cattttccaa aatgactaga acagagcctg
    22441 ttacgagaat cacagctgtt accaaggctc ctagcaggct aaatcgctta tagcccaagg
    22501 tgtactgatt gtcttcttca cgattggaga ttgtttctag aaaagctgat attccaattg
    22561 caatcgcatc tcccaagtca tgcacagagt cagcaagaac agcgctagaa ccaaatactc
    22621 cacctgcaat aaactcaaca atggcataag tcaaatttaa gaaaaaagcc acccaaacag
    22681 catattttgc cttcatattt ctcattcctt tgttataata gatttatgaa caccttgttc
    22741 attatcatta tccactaaaa ctcaaagaaa ggatagaagc aaaacgtcag ctttattcag
    22801 ttctgaacaa tttgccttaa gtgtccatat gactaacatt gaccgccgta tcagcaaaac
    22861 caaaaaagcc atctatcaag cttttataca acttttgaat gctaagggct acgaggccac
    22921 tactgttcag gatatcattg atctcgcaga tgtgggacga tccacctttt actgtcacta
    22981 tgagagtaag gagcttcttc tggaccagct ctgccgctac ctcttccatc atctttttga
    23041 aagagagcaa gccatttcaa ccgaggacta cctcgcccac ctctttctcc attttcataa
    23101 aaaccaagac catatcacca gtctgctatt ttccaaaaat gactacttcc tccgtcaact
    23161 ccataaagag ctagaacacc atgtctattc cgtgctagct gataatttaa aagaagccca
    23221 ccctaatctg cctacttctt acctccaaca cttggtcgtg tccaacttta tcgagacatt
    23281 gacctggtgg ctcaaaaaag gtcaagattt cacagaccag gaagttgtcc aattttatct
    23341 agaccttctc attcctaaaa attgaatata gagtaaagct tcagttgtct tatttctagg
    23401 ttactgagtt ttttatcttt tcaacaacaa aagaggaccc gccgatcctc tttttcatac
    23461 tataaatcct tgattatcaa ctatatctgt tttaatcgaa atctcaaaac agcactttca
    23521 aacatctttt cctagttaag taaatcagta ttttgcttag ctgccttgct ccattgatac
    23581 caaccaacta gactgttaat gagataaatt agatatttcc cttgaatttg caggctttct
    23641 ccccaccaga gatagattga aaagacattg gtagccgccc agaatatcca ctgttcacgg
    23701 taaacagctg tcatgaggat ttgccctacc ccattggttg catctgtgat tgaatcacga
    23761 tagggacgat tggcaccaat agactgataa atgaagccaa aggccaacca ccaaagcaca
    23821 ctaatggaaa gatactttgt ccagcccttg ccgtccagtt tacgcgcgac aaactcctgc
    23881 ttttccttct taaactgtgc ctgataaatc caaactagaa gtccaattgg ctgcatgact
    23941 gtgaagtaaa gtgtcgtcag cacctcacca taaaagcctt tctgtagggc caaaataagg
    24001 taaataacag agttaatcaa gccaaaaaga taattacttg ctcgaccttc cgatacaaag
    24061 attacacaga taatccctgt caagctacaa atcatcccaa tccagtcaac aatacgatgt
    24121 tcgtaaacca actccagcca gagaggaaaa cttcctaaaa ccagcaaata aatccactgg
    24181 gcaaaactac gatgggcaaa gaggtcatcc cagatagcct tcatagttcc tgaaaatcct
    24241 aaatcagcca tagccgcaac catacgacgg taaccacctg acatttcacc tagggttgtt
    24301 ttgatatttt caattttctt ttgcaaataa gtatgcatca tttctccttt tgtttttaaa
    24361 gagccgtgtc tggatagact ttcggacgca acgctctatt agataatgaa ctgcctatac
    24421 acaagatttc taaccttagt cgacatgagc tgaaacctct tatttgttaa gtagttcaca
    24481 aaatattata cacctatttt atgaatagtc aactgtcttt acagtaaaat tttagaaaat
    24541 catgaaaatt ttctctttct ttccatttta agtgacattc agtcattctc acatcaaaaa
    24601 agcccagacg aaattgtctg agcattcttt tatctagtcg tttaaggaag ttgagttcag
    24661 tatgtttaaa gtctctgtcc catcatttct tcaacaaacc ttgttcttgg agaaactcct
    24721 tggctacttg ctctgctgac ttgccttcaa caccgacttg gtagttgagc tggctcatct
    24781 ggttttctgt aatcttacca gccaatgtat taagaactct ttccaactct ggatgtttct
    24841 tgagaagagc ttctttcatg agtggagccc cttgataagg tgggaagagt tgcttgtcat
    24901 cttccaagac ctgtaaatca taacgctcca attccgcatc agtcgaatag gcatccgtga
    24961 tttgaatatc ccctgactga attgcctgat aacgaagagc tggctgcata gtcgccacat
    25021 tgagattgag accatacatt gattgcaagc ccttatttcc atcttcacgg tcgttaaact
    25081 caagcgtaaa gcctgccttc agctgccctt ccactttttt caaatccgaa atggtcttca
    25141 agccatattc ttgagcaatc tttttcggaa cagctacagc ataggtattt tgataagaca
    25201 taggtttgag ataggctaga tgatcctgct tggcaatgcc atcacgcgcc acttgataaa
    25261 cctgctctgg ctcatgactc accttgggtg atggttgaag caaactttca gtcaccgtac
    25321 cggtaaattc aggataaatg tcaatatcac cttttttcag agcttcataa aggaaacttg
    25381 tcgtcccaaa attcggttta acagtcgcag tcatgctggt attttcttca atcagcaatt
    25441 tatacatatt agccaaaatt tctggttctg ggcctatttt cccagcaata atcaagtttt
    25501 ccttctcttt ttgagccaaa agggctggac tataagacag acccagtaat aaagccacca
    25561 aggcaaaacc agagaaaatc gtccgcaatt tcgctttttc cattactttt agtaggaagt
    25621 taaaggcaat agctagcact gcagaagaaa gtgccccaat caaaatcaga ctggcattat
    25681 tacggtcaat tcccaaaagg ataaaggaac ctagtccccc tgccccaatc aaggtcgcca
    25741 aggtagccgt accgataatc aagaccgctg ccgtccgaat cccagacatg ataacaggca
    25801 tggcaagtgg aatttcaaac ttcttgagac gctcccatct ggtcatccca aaggcaatcc
    25861 cagcctcttg cagactcgga tcaattccct tcagcccagt aatagtattt tgtaaaatag
    25921 ggaaaatagc ataaatcact agagctgtca aagccggcaa ggtcccaatt cccatcaaag
    25981 ggataaagag ccccaacaag gccagagacg ggatggtctg gaaaatccct gcaatctgca
    26041 agacccagtc ggccagcttc tcatgatagc gaagaaaaac agccaaggga atcgcaagca
    26101 aaatagctag taacaaggtc aaaagtgaca actgcaaatg ttgagataga gctgtcaacc
    26161 aatcactaaa acgatcctga aaagttgcaa ttaaattagt catgaacact acctccaaac
    26221 aagtctgcta caaagtctgt tgcaggcact tttaaaattg tctcgggatt cgctacctgg
    26281 cgaatttctc catcctgcaa gacagcaata cggtccgcca acttcaaggc ttcatccgta
    26341 tcatgggtta caaaaatcgt tgtcatccca aactctttat gcaattcttt tgtcagaacc
    26401 tgcaactgtt ttctcgaaat agcatccaag gccgaaaagg gttcatccat gaggaaaatc
    26461 ttgggctgac caatcatagc tcggacaata ccgacccgtt gctgttctcc accagataat
    26521 tcactaggta agcgatgccc atactcggct actggtaaac caaccttagc caaaagctct
    26581 tctgttttct tcgtaatttc ttccttgctc caccccttca tttcaggaat gagagcaata
    26641 ttttccgcaa ctgttagatt tggaaaaaga gcaatagcct gtaaaacata accagtagaa
    26701 agacgaagtt cacgctcatc atagtctttg atgcgcttcc catccatata aatatttcca
    26761 tcagttggtt ccaaaagacg gttaatcatc ttgagcatgg tcgtcttacc tgacccagaa
    26821 ggccctacta aaaccataaa ttccccatcc tcaatctgta agttgacatc tctcaagaca
    26881 tccttttctg tgtagcgcag tgctacattt ttgtattcaa tcattctttg tcctcaattt
    26941 aaaacttccc tcgattggtc aagtcttcta ccttaggcat gacttcctta ttatcccaat
    27001 gctccacaat tttcccgttc tttaaacgga agatatcgta cttggcataa gcaacgccat
    27061 caatctgagt ctgaccatag ctaaccacat agtttccttg tcctaagagt tggaaaacaa
    27121 agtcaaaagt gacactatat tcagccacat agtttttata agcagcactt ccttgtccaa
    27181 tctcatgatt atgctgaatc aaatcgtctg ccacataatc actccactgc tctagctccc
    27241 cattttggaa aatttctgtc aagaaacggc gaaccagctt tttattttct gctttcttgt
    27301 ccaaatcctt gatttcaaaa tctccaaaaa tttgatctag ttggtcattt tcaggtgttc
    27361 gatagtagtc aatgacatcc caatgctcaa caatacgacc attctcatcc gcacggaaag
    27421 tatccgtcgt cacccattga gcttctccac cattcagata ttgatgaaca tgaacaaaga
    27481 ccagattgcc atcctcaatg gtgcggacaa tcttaatctg acgctctgga tgacgctcaa
    27541 agaaatctgc aaagaaggct gcaaatcctt ctttcccgtc aggaacacct gtcgaatgtt
    27601 ggatataggt atcccctaca gactgggctt gagcctcagc aactcgtccg tcttgaatgg
    27661 catggatgta taggttgtga gcatttttca cttgttgtga catattctaa acctcatttc
    27721 ccttctcttt cagattcgcc aaaattcttt cttgaaaacc ttcaaattgg tgaatttctt
    27781 cctctgaaaa tcctttgtaa aagatagtat ccaatttctg actgacacga tgccccactt
    27841 ctttctggga cttgcctaac tccgttaaaa ctaaatactt cttacgcttg tcttttccac
    27901 acggactaac aattacaagc ttttgttcct ctagcttttt tatcatagtc gtcagcgtat
    27961 tattcgcaag cccagtcgca agcgcgatat ctgtcgcagt tgcgcagcca gtttcactat
    28021 tccataaaac cgctaaaatc ttgccctgtt cacccctata aagagcctca ggatcttgac
    28081 tcagtaactt ttgaaaaatc cgcccattca acaaacgaat atgatgggct agcaaatgac
    28141 tatctttcat aacacctcca atttatttcg atatcgaaat gaataaaata attgtaacac
    28201 tcatcgttct aactgtcaac tatttcgatt tagaaataat ttttgataat tatccacacc
    28261 accatactcc ggctcaacta acttttaacg agagtttcta aactccttcg tcctccagtc
    28321 tacaaaagcc ttccatccgt actatcctat attttatgag gggacacatt tttcctatca
    28381 gagcatttat tttaaagata gaagtaaatc ataattgctt ccatctgttc ttttatagta
    28441 tattgaagtt ggactagggc actgtatctt ctaaaacatt gatagaaagc gatttgaatt
    28501 tcccaatcaa tttgttcgtc tttatagcat ttcgaaactg gaataggaca ccatgactgc
    28561 taaaagattt ctataaattc atttgatttc cctaatcaat ttgttcatat cttatttcat
    28621 tccgctataa tttcacctta ccctatcttt ttcgtagcac ccttcaaaca gcctatcccc
    28681 taccgtttga cgattcctca cttcactcca cttccattac agaagtttct tcactactat
    28741 ggtctcggct gacttctcat gattccttgt tactactatt tgaacgctca cgagataggt
    28801 cttataaaaa atgctttgat ccacaatgga atcaaagcat tttaaaaagt tcctcataca
    28861 taagtgcaga agtcgcagtt cctctgtact tggcttcttc tcttttgata aagcgagcca
    28921 agttgagcaa ctcaggtgct ggatgtttgg gatttaggag caattcacga ttgaccaggc
    28981 ctgagagacg aactgcctgc aattgctcat ttgtagtagg cagtttttta gtagtctcta
    29041 ggagagcagc aactaaatct tcactcaaat catgtcgagc atgattgtaa agatctttta
    29101 taaggctttc taggtttggt tctgccatcc ctaccacctc ccttatggtt taataatgtt
    29161 taatcaaatc aaccgttgaa cgatccaatt tcttcaccaa ggcttgtaag aaagcttgcg
    29221 cttctaggaa gtcatccatt gcatagaggg tttggtgaga atggatataa cgagcgcaga
    29281 caccgatagt tgttgatggg acaccaccat ttttcagatg agctgcacct gcatctgttc
    29341 cgcctttacc acagtagtat tggtacttga taccagcttc ttcagccgtt gtcaaaagga
    29401 aatccttcat ccctgggaga agcaagtgac ctggatcata gaaacgaatc aaggttccat
    29461 ctccaatctt gccttgacca ccgtagacat cacctgctgg tgagcaatca actgcgagga
    29521 agacttctgg gtcaaacttg gttgtagagg tatgagcgcc acgcagacca acttcttctt
    29581 ggacgttaga acccagatag agttcattgc cgagtttttg acctgataaa gcttcagcta
    29641 gctcgcttac catgaggaca ccgtagcggt tatcccaagc ttttgagatg atattttttt
    29701 cattggctgt caaaattgca gaactatctg gtacgatggt atcaccagga cggatgccaa
    29761 aactttctgc ctcagccttg tccgcaaaac caccatcaaa aacgatatcg gcaatggctg
    29821 gcatggttgg tccccccttt ccacgagtca aatgcggagg aacagaacct gaaatcacag
    29881 gaatttcatg accatcacga gtcaagagtt tgaaacgttg gctgctaacc accatggggt
    29941 tccagccacc gatttctacg acacggaagg taccatctgg cttgatttcg ctgaccataa
    30001 aaccaacttc gtccatatga gaagcgacca agacgcgcgg tgcatccaca gcttctgaat
    30061 gtttgatacc aaaaatacca cccaagccat ctgtcaccac ttcatccaca tgcggtgtca
    30121 acttttcacg aagataagca cggacaggcg cttcatgacc tgagactgca gcaagttctg
    30181 ttacttcttt aatttttgaa aataatgttg tcatttcagt tccttctttc tttcatccat
    30241 tttaccactt tttataggag aaggatagtg ggaaggtgga tttctaagtt agtatcttag
    30301 tcctgctcta tcttagaaaa ggatagtatt ctcttgcatg tagtgcaaaa tctagtaaac
    30361 attccaaaat taactcgaat atttatttcc aaacaaaaaa caatacacca tcaaagttgt
    30421 ttggattttt catgaaattt acagaaaata gttgacttcc ctttcttctt tctttaaata
    30481 tatagttggt tgagtttgga atagtacgct gtagctgcta aaacatttct agaaattaat
    30541 ttgactttcc taatagagtt gttcatatct tatttcaatt tactatagta caaaactaga
    30601 aaaggaaaaa atcatgacca aaacaattct tgttacaggt gggactagct acattggctc
    30661 ccataccgtt aaagctcttt taaatgctgg ctataaggtg catgtcctcg ataatctctc
    30721 cacaggaaat cgagcggctg tggatagtcg tgctagcttt aaagaactgg atgtttatga
    30781 tgctagcgcc ttaaaggttt acttagaaga aaatcagatt gatgctgtcc tccattgtgc
    30841 aggtgaaatt gttgtgagcg aaagtattga aaatccaagt aaatacttca ctgccaacgt
    30901 tgctggtatg aaccaagttc tcaaagtctt atctgaagtt ggcattcaaa aaatcatgtt
    30961 ctcttcgact gcttctctct atggaaataa ctgtattgac aagccggtga ctgaagatac
    31021 cctgctcgac cctgtcaacc cttatgcaga gacaaaactg atgggcgaac gaatgattta
    31081 ctggatggcc aatcgctacg actggaaata tgttattttc cgttacttta atgttgctgg
    31141 ggctgaaatg gatgcttcaa acggtctgcg tgtgaaaaat ccaactcaca tcattccaaa
    31201 tatcaacaag accgcattgg gacaaaatga tagcttaaaa atatttggag atgactacga
    31261 tacacgtgac ggttcatgta ttcgagatta catttatgtc ttggaccttg cataggctca
    31321 tgttaaagga atgaactacc tatttcaaga agacagttct tctcaaatct ttaacttagg
    31381 aactaaaaaa ggctatacta tcaaagaaat ctttaaaact gctgaagaat tactaaatta
    31441 aaaaatccca cacgagattg ttgctcgacg tgctagtgac ccagccagtg tcctagcaga
    31501 tgcttcaaaa gctaaacaat atcttgattg gacggctagc tactccctcg aagatattat
    31561 tttatcagat taaggtaaaa acattttaga ataggataac aaaggagaaa gttttgttgc
    31621 tctctccttg ttgtatttat taaattgtca gaaattttat tgacagatta gaaaaataat
    31681 gttacattta tatccgcagg tatctttcga taccaaatct acatgaaggg acggggtatg
    31741 aaactttctc attatttaat tggcttactt ctactcctag tctttctctc tattagcatt
    31801 gggaccagtg atttttcatg gggaaagcta tttgatttcg accagcagac ctggctcctc
    31861 tttcaagagt cccgtctccc aagaactatc agtattctcc tgactgcctc tagtatgagt
    31921 atggcaggcc ttctcatgca gactattacc caaaatcagt ttgctgcacc gagtacagtt
    31981 ggaacgactg aagccgccaa actgggaatg gtgctgagcc tttttgtctt tccatcggct
    32041 agtctgaccc aaaagatgct cttcgctttt gtttcatcca tcgtattcac cctcttcttc
    32101 ctagccttta tgaccatttt tactgtaaag gaaaggtgga tgttgcctct gattgggatc
    32161 atctatagcg ggattatcgg ctcagtcaca gaagttatcg cctatcgttt caatctggtt
    32221 cagagtatga ctgcctggac ccagggctcc ttctccatga ttcagaccca tcagtatgag
    32281 tggctcttct taggcctcat catcctgata accgtttgga aattatccca aaccttcacc
    32341 atcatgaatc tagggaaaga aaccagcgag agtttgggga tttcctactc cctacttgaa
    32401 aaactggccc tctttctggt ggcgctaacg acaagcgtca ccatgattac cgtggggggc
    32461 ctaccatttc tcggagttat cgttcccaat cttgttcgca agcgctatgg agataatcta
    32521 agtcaaacca aactcatggt cgcactggtt ggtgccaatc tagttctggc ttgcgatatc
    32581 ctatcccgag ttctgattag gccctatgag ttgtctgtca gtctcttgct aggaatcatc
    32641 ggtagtctcg tctttatcct acttctctgg agagggggac gaaaagatgc agactaaaag
    32701 caaacatacc aagctcttct ggattctcat tattcttgcc atcggagctt gtcttctcta
    32761 cttttggccc atcactcact tgtcagcctt tgcttggaag ttgcgttccc aaaagatcat
    32821 cgtttatctc ttggtagcca tcgcgactgg gatttcgacc attagttttc aaaccctgac
    32881 ggaaaatcgc ttcctgacgc ctagtatttt aggaattgaa tccttctacg tcctactaca
    32941 aaccctacta ctggtttttg aaagcaagtt tcttcaactt ggcaaatccc ctatcttaga
    33001 attcctagtc ttacttcttg tccagtccct cttctttctc gccttacaag gttacttgaa
    33061 gacactgatg aagcaagacc tggtcttcat cctgctgatc tgtctagcgc tcagaagtct
    33121 ctttcgaaat atcagcacct tccttcaagt cctaatggat ccaaacgaat acgataaact
    33181 gcaaaatagt ctttttgcct cctttcaaca tctcaacact tccatcctag ccatcggttc
    33241 tctgatcatc ctcgctttga caatcttttt ctttcgaaaa gcagtcgttc tagatgtctt
    33301 gcacctgcaa agagaaacgg ctcagatatt gggactcgat gttgaaaaag aacagaaaga
    33361 gctcctctgg ggaatcgtgc ttttgacctc aacggccact gccttggtag gacctatggc
    33421 cttcttcggc tttatgctgg ccaacctcac ctacctgatt gtcaaagact atcagcacaa
    33481 gttactcttt atagtggcca ttctggttgg atttattagc ttaaccttgg ggcaagcctt
    33541 gattgaacga gtctttgcac tggaaattcg tatcagtatg atcattgaga gtgtgggtgg
    33601 cttcttattc tttatcttac tatataggag gtctcgtcag tgaaactgga aaacattgac
    33661 aaatccattc aaaaacagga tattttgcaa ggcatttcgc ttaaagtcag tcctcaaaaa
    33721 ctgactgcct ttattggtcc aaatggtgct ggaaaatcga ctctcctctc catcatgagc
    33781 agactaacca agaaagatca gggagttctc agtatcaaag gacgtgaaat cgagagctgg
    33841 aattcgcaag aactggctca agaactaacc atcctaaaac agaaaatcaa ttaccaagcc
    33901 aaattgactg ttgaagaact ggtcagtttt ggacgttttc cctacagccg aggtcgactt
    33961 agatcagaag actgggaaaa aatccgagaa actctgaact atttggaact gaccaactta
    34021 aaagaccgct acatcaatag cctgtcaggg gggcaactcc agcgcgtctt tatcgctatg
    34081 gtactggccc aggatacgga ctttatcttg ctggacgaac cactcaacaa tctcgatatc
    34141 aagcaaagcg tcagcatgat gcagattctt cgacgactgg tggaggaact cggcaagacc
    34201 attatcatcg tcctccacga tatcaacatg gccagtcagt atgcagatga aattgtcgcc
    34261 ttcaaggacg gccaggtctt tagcaaggga agaaccgatc aaatcatgca ggctgaccta
    34321 ctcagtcaac tttatgagat tcccatcacg ctagctgata tcaatgacaa aaagatctgt
    34381 atctatagct agtaacataa aagctcaagt tagagaacct tcagtctctt agtcaataag
    34441 atcaagagac tccctaaatc gttatcacat tttaaaaagg agaaattatg aaaacatccc
    34501 ttaaacttta tttcactgcc ctagtggcca gcttcttgct cctacttggt gcatgtagta
    34561 caaactcaag cactagtcag acagagacca gtagctctgc tccaacagag gtaaccatta
    34621 aaagttcact ggacgaggtc aaactttcca aagttcctga aaagattgtg acctttgacc
    34681 tcggcgttgc ggatactatt cgcgctttag gatttgaaaa aaatatcgtc ggaatgccta
    34741 caaaaactgt tccgacttat ctaaaagacc tagtgggaac tgtcaaaaat gttggttcta
    34801 tgaaagaacc tgatttagaa gctatcgccg cccttgagcc tgatttgatt atcgcttcac
    34861 cacgtacaca aaaattcgta gacaaattca aagaaatcgc cccaaccgtt ctcttccaag
    34921 caagcaagga cgactactgg acttctacca aggctaatat cgaatcctta gcaagtgcct
    34981 tcggcgaaac tggtacacag aaagccaagg aagaattggc caagctagac aagagcatcc
    35041 aagaagtcgc tactaaaaat gaaagctctg acaaaaaagc ccttgcgatc ctccttaatg
    35101 aaggaaaaat ggcagccttt ggtgccaaat ctcgtttctc tttcttgtac caaaccttga
    35161 aattcaaacc aactgataca aaatttgaag actcacgcca cggacaagaa gtcagctttg
    35221 aaagtgtcaa agaaatcaac cctgacatcc tctttgtcat caaccgtacc cttgccatcg
    35281 gtggggacaa ctctagcaac gacggtgtcc tagaaaatgc ccttatcgct gaaacacctg
    35341 ctgctaaaaa tggtaagatt atccaactaa caccagacct ctggtatcta agcggaggcg
    35401 gacttgaatc aacaaaactc atgattgaag acatacaaaa agctttgaaa taagccactt
    35461 caaactcaat ttaatactca atgaaaatca aagagcaaac taggaaacta gccgcaggtt
    35521 gctcaaaaca ctgttttgag gttgcagatg aaagctgaag cggtttgaag agattttcga
    35581 agagtataaa attttgacat tctaaaacgc atcctatcaa gtttactcaa acctgatagg
    35641 atgcgttttt atcattttga aatctattta ccccacctca ttccccttct tgatttcgtt
    35701 taagggaaaa gtggtctgga acggggtcct tacctgtttt cgaccaggga tgacaacgta
    35761 aaatccgagc caagcccatc aataccccct taaaaccatg tttttcaata gcctgaatca
    35821 tgtagttgga acaagtcagc tcaaagcgac aagagggggg aaagactggt gagataaaac
    35881 gttggtaaaa gcgcacaggc gctattaaaa ttcgtttcat tatttcttag ttacagccat
    35941 ggtgtgtagt tggctgattt cttttttatt aagacgacgg gattctcctg gacggagtcc
    36001 tgtcaagtct aggtgtccga aacgagtccg agacaactta tctacttgga gaccaacagc
    36061 ttcaaacatc tttttaacct gatggttacg cccttcatgg atggtcaact gcaccacaga
    36121 gcgatttttg actgggtcca ctttgagaat ttcataaaca gctggcttgg ttttcttacc
    36181 atcaatctca agaccacggg tcaaggggcg gagattgtcc ttattggcca cacctttaac
    36241 acgcgcgaca taaaccttgt caatctcatt acgagggtga atcatctcgt ctgtaaagtc
    36301 cccatcattg gtcaaaatca agacacccga tgtatcccag tccaaacgac ccacagggta
    36361 aatacgttct ttgacattgg gcaagaggtc gacaaccgtc ttgcgaccct tgtcatctgt
    36421 tacactggaa atgacaccgc gtggtttgtt aagcagataa tagacctttt cttcgttgta
    36481 gataggttga ccttcaactt cgaccttgtc gcctgacttg atagtggttg ctagttcacg
    36541 caccacttgg ccgttaaccg tcaccaaacc ttgcttgatc agctcttctg cttttctcct
    36601 actggccaca cctgcgtggg caatatactt attgattctc atcttcttct atcctttcac
    36661 caaataattg gctttcttgg gcttgaatct caagctcatc aatcactggt aattcttcta
    36721 aatggtttat ccccatgtaa tctaggaaat aatccgtagt cacatagagg ttggggcgcc
    36781 ccaatacttc ctttttcccg tcttccttta tcaggtcaaa agcttgcaac tttgccaagg
    36841 ctccactcga gttaacccca cggatggcat caatttctat ccgcgtaatc ggctgtttgt
    36901 aggcaataat ggacaaggtc tcaagggcag cccgagacaa gctctggttg ataggcgcct
    36961 tagagtattc cttcaaaatc tctgcaaatt gaggcttggt caccaatcta taagcaccac
    37021 ttgtctcaat caaagccaaa ctggaatctg ggtccttttc atacttctgg gctaattttc
    37081 ctaaactttg ctggatgcct gtcggtggca gagagaggag ttcagctaac tggcggaccc
    37141 gaatcccatc ttcacccgct acaaacaaga gcgcttctat ttttgctaaa gtactcatct
    37201 ttcctctcta tcaagtctag ctttgaggca cttgactttc ttccttcttt tccatgagat
    37261 agatatctcc aaaactctcc tcttgcacga ggatcagctc ctgggttttg attaactcta
    37321 gggttgccaa aaagagggtg atgacctctt ggacattctg ggcttccttg aacaaatcct
    37381 gcaagcgcaa ttgatctcgt ccaatcaagg attctttcac aataatcatc atgtcctcaa
    37441 tcttatactc atcccgcaag atagtcgtgt gattttgtgc aaactcctct tttttcttgg
    37501 ctaggatatt tgaaaaagcc aaaaagaggt caatggtcgt cttgtcatgc acaagctccg
    37561 catcttcgta aatcaactct atcggcgctt tggaataata ctgggcccgt tcttggtgct
    37621 tggcttccaa gtgctcaccc aagagcttga acttgcgata ttcttcgatt tgagagagga
    37681 ggtcctgctc caggtcatcc cccaagtctg tcacttctgc taccttcgga aggagtttac
    37741 gactcttaat cagcatgagc tgactagcca tgaccatgta ctcacccgtc acttccagac
    37801 gcatggcctg cagggttgag acataggcta gatactgttc gatgacttcc gtaatgggca
    37861 catcgtagat atccatctgg tacttagaaa ccagatgcaa gagtaagtcc aggggtcctt
    37921 caaaatcttt taatttaata tccattatct atatttttct aaggtcagga ctgtttttaa
    37981 tcctaatttt tttgcaattt cgtacaaatc gaccttgttt tctatttgtc ttagaataaa
    38041 ctgttcacgt aagacttgag ctgataagga tggaaaacct ttctccttga caaaagattc
    38101 taactgacga aaggcccact gacgagaata gggtttccct cctctttcaa aaagataggt
    38161 ctgccccatc aagggttcca attctgaaag caaggccgtg ggaatggtga caatcctccg
    38221 ttgggaagcc ttgctgattc gcaacacctg aaaatccaga ttgatgtccg caaccttgat
    38281 ggctaaaatc tcactgggca agagccccat ttctaggatt aagagcgcta gcaagcggcc
    38341 ctctggatgg tcgctttcct gccaaaaaga gtctaggtat agaatctctg gcttttccgt
    38401 cttcttttca gcttgtttgg ctaattccaa gcggtaaaag ctgtccacct ctcctttttg
    38461 atagagaaag tagagaaatt ggttacaggc cgaaatcttt cgcttctggg cgctgatttt
    38521 tagattggct agctgggctt ggtaaatctt gagactggtc tcagaaatcc gctcacctac
    38581 catgtctaaa aattgctcca aatcatactt ataggactgc ttggaattga cagacaagct
    38641 ctgcttttcc tctaaaaagg ctgaaatcct gtctctcatt ggcatcgaat ctcatattcc
    38701 ttactaaagt catgcaagag ggcattaacg gccttgagaa tggacttgcg cgtaataatc
    38761 ccttggaaaa taccctttgc atccacaacc ggtaagaagg actcatctac tagcttgtgc
    38821 aagacctccg taatggtgaa atcaggcgaa acaaccgcta cgtccgtttt tgtcatatga
    38881 acgatatccg tatccgccat gatttcttgg ctcaagtcat gctccatctg ataagccata
    38941 atatctctga gtccaatcgt cccaacaaac tgtttttcat ctgtcacaac gggaacacgg
    39001 gtataggtca tctgactgag caagagggtc gcatgatccg cattgtgggt atcaatcaac
    39061 acagctagat ttttagcagg ggtcaaaaaa gtttcctcct gccccaacaa gaaagtctca
    39121 aactccttgg caatcatcgg ctaaactcct tggacaaacc tggatacacc tcgtgatctc
    39181 gtgtcaaaaa gtccactttg aagtaactat catcaatctc cacacgagca tagagacatt
    39241 ctctgatggt acctcgtggt tggctgatag aacctggatt tagaaagagg atcttgcctt
    39301 ccaaccaagc acttggcaca tgcaagtgac catagaggca gatagcggcc tcttcctcct
    39361 gagcccagta gtccaacttt tgaaagttga aattgatgtc aaacaagtga ccatgagttt
    39421 ggataatctt ggtcgaacca agctcagtca ccagacgttc tgggtagccg gcgtagaagt
    39481 ccatgttccc tttaacaacg cggatgccct cccaaagtgg agaatccgga cgtagttcag
    39541 aatcgccgtt atgaaaaacg gcatcgactt tgcccacata gcgatcacgg acttcttcca
    39601 caatcaagct atcgccatgg gaatcgctca ttacaatgat ggtttgcttt gccatgatgg
    39661 aaatacctcc aaaagtttct taacggctaa ggcacggtga gattgactat ttttttcttc
    39721 cagggttaat tcagctgatg actcacctgt ctctcctaca aggaagaggg gatcatagcc
    39781 aaagccattt tcacccttag gttcaaagtt aatataacct gaccagtctg cttcaacaac
    39841 taaactttcc ttatttgggc tggctacgac tagggttgtg tggaactgag ccgagcggtc
    39901 cttgagttca aagaccatgg ccaattcgtg caagagtttg gcattatttt cacggtcagt
    39961 tgctcccaca cctgcgaaac gagctgacca gacgcctggt aagccaccaa ggacatcgac
    40021 tttgagacca gaatcatctg ccaaaaccat cttgcccgtt aattgagaaa tggtttctgc
    40081 cttaaggcgg gcattttctt caaaggtcat acctgtttct gctacttcag gcaggtcagg
    40141 gtagtcatta agattttcca catcgtagcc taacttatca aagatagctc ggaattcctt
    40201 ggttttgcct tcgttacgag tcgcaatcaa caaggtttct ctgaccttgc ttgtctcaaa
    40261 gaaatcatga acattgaccc cttctttagg caattctaca tacaagagtt gcccattttc
    40321 aaccaagagc agggccccat gacgttgaat aacttccaag tttcgtccca tttcttgatt
    40381 aagcatatct accacaaagg agaacaaacg gtaggtagaa ccatagcgta aaacagtaac
    40441 tgaaagcggg aaaccatact cttcatctcc aaatattttg gagaattcaa acagaggaaa
    40501 atctgtctga tagtcgctca aactgttaac gccaccaaaa atactataag acccaacata
    40561 ccagtcctgg tcatccttat attcataaat tttatttgtc ataattctac atgctccaca
    40621 tgaatctctt tttccagcca ttcttcacca atttgtgcaa aactttggct actggctgtt
    40681 gtgtaaaaac ggtgatggag tggtccagca tcgcgaccac gattgatttc aaaataattg
    40741 agtaagactg agatatcccg tacgcactct gccccactat cgatgagctg aacctttggc
    40801 cccatcacat tttggataat agggcgaagg agtggataat gagtacagcc caaaatcagg
    40861 ctatccacct ttccaaccaa gggacgcagg gtttcataga ccaccttctt ggtaacactg
    40921 gttgacaggg cacctgactc aaccaaggga gcaaacttgg gacaggccaa gctctccacc
    40981 tgtaagtcgg gatccagatc atggattttc tgacggtata tgtctgattg taccgtcatg
    41041 ggcgttccaa tcactccgat tttcccacct tgactggact tgatggctgc cgaagctcct
    41101 ggcaaaatta cacccaagac aggaatatct agttgagcct tgatttcttc ccagacgacc
    41161 gcagtcgcag tgttacaagc aatgacaatc attttgacat ccttggtcaa gagaaagttg
    41221 accagctgcc aagtatattc accaatttgc tcagcaggac gggggccata gggcgcccgc
    41281 gccgaatctc caatatagac gatttcttca tggggaagct ggcgcatgag ctcgcgcaca
    41341 acggtcaagc ccccgacacc cgaatccaaa aaaccaattg gtcgattatc catacagtct
    41401 tctttctagt cttttttctg attgatcgtt cattatgatt ccttgtactg aacgaactga
    41461 ccgacagctt gatagaatgt caactacctc tctcttaatc ataatcaaat atcaatagaa
    41521 tgcaaggaaa aagtctcatc cctaaaccat aaccaacata gtgagaagtc tggaactttc
    41581 atcccagact tttccttatt tattttttct ttttattgtt agcaagggct gccttttgtt
    41641 ggcggatgat ttggtggtaa acttgttgta ccttagcttc gcttggtttt tgaccatttg
    41701 cactcaaaag agtacgaact gcttcagcat tcaaacgtgg gttgtcagca aattcttttt
    41761 cgatttgctt acgaaccaag tacattcctc caagagctcc tcctagaaaa gctagcacaa
    41821 tcaatacaat tgctaaaagt aaatccatca tttttctcct gtttcttttt gagtcccttt
    41881 cattatacca caaactagcc accctgacca gtttgttcta cactttcaag gatggaatag
    41941 acagcaaaaa gaaccctgct tttctgaaaa agaggtctcc ttgctaagtt tatactcaat
    42001 gaaaatcaaa gagcaaacta ggaagctagc cacaggttgc tcaaagcacc gctttgaggt
    42061 tacagataaa actgacacgg tttgaagaga ttttcgaaga gtattaattt acataaatag
    42121 ccagtgtttg atagggtttg agtagaattt tctcagacac ttctgcatct tcatagtttg
    42181 aaatcaaaat ctgtccattt tggtagactg ctggcaagtc gatttccact tctttggcat
    42241 aaaagttatt gagcactagt aacttttgat cctcaaactg gcgttcaaaa gcgtagactt
    42301 gtttgctatc ttcaaaggct ggtttgtaat ttccttctga aatgattggc atttccttac
    42361 gcagtcgaat caagtcttga tagaaggtga aaatcagacc tttgatttca ttttctacat
    42421 tgatatcttt ataggattta ccaactttca accatggagt gcctgttgaa aatcctgcat
    42481 tttcagaagt atcccactgc atgggaatac gtgaattatc acgcgactta gcttgaataa
    42541 tctggaaggc ttcttgctga ctctttcctt cttctaagag catctgatag gcattaagcg
    42601 attcgacatc cacataatca gccatagaat catagtctgg gtcaatcatc ccgatttcct
    42661 cacccatgta gatataaggt gtcccacgtg acaggtgaat gctggctgct agcatggtgg
    42721 ctccttcctt gcggaagttt tgaatatcga caaaacggtt caaggcacgt ggttgatcgt
    42781 gattgttcca aaagagggca ctccaaccgt ctttatcact catttcctta ccccaactat
    42841 ggtaaagact cttcaactct tcaaaatcaa agggagccaa ggtccacttt tgtccatcct
    42901 tatagtccac cttgaggtga tgaaaattaa aggtcatgga taattcctga cgatcaggcg
    42961 acgaatagag gacacagttt tccatggtgg tagaagacat ttccccaact gtcataaagc
    43021 tatcgtcaga tccaaaagtg gcttggttca tcatacgcaa atagttatga acgatgggtt
    43081 tgtctgtata agctggcttc ccttcatttt caggacagtc cactgaaacc tcgtccttac
    43141 cgatcaaatt gatcacatca aatcggaaac ctttgacacc cttgtcgcgc cagaaattaa
    43201 caaccttgaa aagctcctta cggacattgg aattgcgcca gttaaggtca gcctgggtct
    43261 catcaaatag gtgaagatag tatttcccag tatccccgaa aggcgcccat gcagaaccac
    43321 caaacttaga ctgccaatct gttggttggt cttggatgaa gaaaaagtct tgataatact
    43381 tatcaccagc tagggctttc tgaaaccatt catgctctgt cgaacaatga ttaagtacca
    43441 tgtccagcat aaagtcaatc ttgtgctctt taccgacaca caccatttcc tcaaaatcag
    43501 ccatatcacc aaaaagagga tccactgcca tataatctga aatatcgtaa ccattatccc
    43561 gttgagggct tggatagaat ggattgagcc agaccatatc cacacctagt ttggctaaat
    43621 agggaatttt ttcgataatc ccacggaaat ccccaatacc gttttcagtg gtgtctttgt
    43681 aagattttgg atagatttga tagactactt ttcctttatc aagtgtcatc tgtttctcct
    43741 tttctgataa aagggaggaa gcagtcttcc gtccctattt gtgctatttc aattatactc
    43801 aatgaaaatc aaagaacaaa ctaggaagct agccacaggt tgctcaaaac actattttga
    43861 ggttgcagat agagctgacg tggtttgaag agattttcga agagtattag attcgtgtag
    43921 cgaccatgag agatgctcca gcttggatcg ttgtcggata agttccggga atagtcgctg
    43981 tataagcatc ttggttggtg atgataacag gagtttctgt caccagacct gcagccttaa
    44041 tgacatccat atcaaaacga atcagttgct gaccaactgt aacgtgatct ccttggacta
    44101 caagactttc aaaacctttg ccatcaagac ctactgtatc cataccgatg tggatgagca
    44161 attcaactcc ctcgtcagag acaatgccga tggcatgctt ggtagggaaa agaaccgtca
    44221 ctgtcccatt aactggagag gtcaactcac cttggcttgg ttcaatgacc agaccttgcc
    44281 ccatgacacc tgatgcaaaa ataggatccg tcgcttgact caattctttc acttggccag
    44341 ttagtgggct gataatttct accgaagtaa gttctactgg ttcatggttc acaaattctg
    44401 cttcttcttg agcaacgaat tctgcctgca agttcgtatc gccctctgtt tttgtaaaga
    44461 gaccagcctt gcggaagaag aaagtcaaga gcattggaac aacaatcgca actagcatag
    44521 ttcctgcaaa tggcagcatg tattgaggtt gaatagagag aatacctggc aaaccaccga
    44581 taccaataga agccgcagtt acattaaaag taacggataa catgcctgca agggctgaac
    44641 cagtcatccc agcaacaaat ggataaatat attttacgtt aaccccaaaa agagctggtt
    44701 ctgtaacacc gagataggct gaaatggttg caggaagtga aacctgagcc tcacgctcat
    44761 catggcgatg catgaaataa taggcaaaca cggctgagcc ttgagcaata ttagaaagag
    44821 caatcatagg ccagagggca gtgccaccag catccgcaat caattgtgta tcaatggcat
    44881 tggtcatgtg gtgcagacct gtgatgacaa atggagcgta gagggcacca aaaattgcac
    44941 cgaagagcca tttaactgga ccagttaaac ctgccaagac aactgatgaa agtccttgtc
    45001 caattgtcca accgattggt cccaaaacag tatgagccaa aatcaaggct ggaatcaatg
    45061 acaagaaagg tacaaaaatc atggaaatga cttctgggat atgtttgcgc cagaaaattt
    45121 caagataaga cagactcaaa cctgcaagca aggctgggat aacttgggct tggtaaccaa
    45181 tacgattaac agtaaaataa ccaaaattcc aaacccagtt tgctgcgata tcagctgctg
    45241 gcgttgaagc aaccgcatag gcattgagca actgaggcga taccaaacag attccgagaa
    45301 caattcccaa aatttggctg gttcccatct tacgagaaac agaccaagta atccctactg
    45361 gtaagaactg gaagatagct tcaccaggca accagaggaa gtgattaacc cctgcccaaa
    45421 actgagagga ttctgtgatg gtcttgccat ccaacatcga ccaatggaca ccttccaaga
    45481 cattacggaa accgaggatc aatccaccaa caatcaaggc tggaataatc ggagtaaaga
    45541 tttccgccag agtggtcata acaccttgga ccacgttttg attactctta gctgcagact
    45601 tggctgcttc tttggaaaca ccctcaatac ctgaaacggc tgtaaaatca ttataaaaga
    45661 tgggcacgtc atttccaatg attacctgaa attgacctgc atttgtaaaa gttcctttaa
    45721 cagctggaat tgactcgata gctttaacat tagccttctt atcatctcct aaaacaaagc
    45781 gcatccgtgt cgcacagtga gttacggcag tcacattttc tttgcctcca attgcctgaa
    45841 gcagatcttt ggcttcttgt tcaaattttc ccatttgatt ctccttatac ttgtcaaagc
    45901 aagtttgatt tgataagtat attgtaaacg ataccaaact tgtctgcaac ttgtttggta
    45961 taaaaagggt gtttttttgt aaattttcta gcacttgtat ggtaaaatag atacaggtgt
    46021 tcattaaact agactaaaaa cctatttaag caggcaaaat gaagaaatac caacaattat
    46081 ttaagcaaat ccaagaaacc attcaaaacg agacttacgc tgtcggagat ttccttccta
    46141 gcgagcacga ccttatggag caatatcaag tgagtcgtga taccgtccga aaggccctgt
    46201 ctctcctcca agaggaagga ttgatcaaaa agataagagg gcaaggttct caagtcgtca
    46261 aagaagaaac cgtcaatttc cctgtatcca acctaaccag ctaccaagaa ctagttaaag
    46321 aacttggact gcgctctaaa accaacgtgg tcagtctgga caagattatt attgataaaa
    46381 aatcctcact gataaccggt ttcccagagt ttcggatggt ttggaaggtg gtccgccagc
    46441 gtgtggtgga tgatctggta tccgttctgg atacggacta tctggatatg gaactaattc
    46501 caaatctcac tcgccaaatt gctgagcagt ctatctattc ttatatagaa aatggcctca
    46561 aactccttat tgattatgct cagaaggaaa tcaccattga ccactcaagc gaccgagaca
    46621 agattctcat ggacattggc aaagaccctt atgtcgtttc gattaaatca aaagtctatc
    46681 tccaagacgg acgccaattt cagtttaccg aaagtcgcca taagttagag aaatttagat
    46741 ttgtagattt tgcaaaacgc aagaaataaa agactgagac accagatctc agcctttttc
    46801 ggctctataa tatttgtagt gggtaacccc cctatggata ttatggagcc tattttgtgt
    46861 agaaaaaaag tcccatatga cctataatga aaagcgacaa aacaactcat tagaaagatt
    46921 catatggaac aattacattt tatcacaaaa ctgctcgata ttaaagaccc aaacatcaag
    46981 attctagaca tcatcaatat ggatacccac aaagaaatta tcgctaagct ggattatgag
    47041 gctccatctt gccctgattg tggaagtcta atgaagaaat atgactttca aaaaccgtct
    47101 aagatccctt acctcgaaac aactggtatg cctactagaa ttctccttag aaagcgtcga
    47161 aagcgtcgtt tcaagtgcta tcattgttct aaaatgatgg tcgctgaaac ttctatcgtc
    47221 aagaaaaatc accaaattcc tcgtattatc aaccaaaaaa ttgcgcaaaa gttgattgag
    47281 aagatttcta tgaccgatat tgctcatcag ctggccattt caacttcaac tgtcattcga
    47341 aagctcaatg attctcactt tgagcatgat ttttcgcgtc ttcctgagat tatgtcctgg
    47401 gacgttgaaa cagtccgggg agtgactgtt tcaatcggga gatggagatg agctttattg
    47461 cgcaagattt taacaatctc aatatcatca ctgttcttga aggtagaaca caagctgtca
    47521 tccgagatca ctttcttaaa tatgatagag ccgtccgatg tcgcgtcaaa atcattacta
    47581 tggctatgtt tagcccttac tatgacttgg ctaaacagct tcgctttcaa atttctaggc
    47641 tcaggctgaa acagtctccc aggctgtttc actcccgaat gctaaaatcg ttcttgatcg
    47701 ctttcacatt gtacaacatc ttagccgtgc tatgagtcgt gtgcatgtcc aaatcatgaa
    47761 tcagtttcat cgaaaatccc atgaatacaa ggctatcaag cgctactgga aactcattca
    47821 acaggatagc cgtaaactga gtgataagcg attttatagc cctacttttc gcatgcactt
    47881 aacaaataaa gaaattcttg acaagatttt aagctattca gaagacttga aacaccacta
    47941 tcagatctat caactcttac tttttcactt tcagaacaaa gaccctgaga aatttttcgg
    48001 actcattgag gacaatctga agcaggttca tcctattttt cagactgtct ttaaaacctt
    48061 tctcaaagat aaagaaaaga ttatcaacgc ccttcaacta cactattcta atgccaaact
    48121 ggaagcgacc aataatctca tcaaacttat caagcgcaat gcctttggtt ttcgaaactt
    48181 tgaaaacttc aaaaaacgga tttttatcgc tttgaacatc aaaaaagaaa ggacgaaatt
    48241 tgtcctttct cgagcttagc tgacttcaac ccactacagt tgacaaagag cctaatttcc
    48301 ataaaaattg acatggaaat tataaaacca ttactagttt agtccttttt gataacgtgc
    48361 caattcggct tggttcgccc aaacatagtg acctggacga atttctacca tagatggctt
    48421 atcagtctca tagtcgtgtt gacttggatc gtaaaccttc aagaccttct tacgttccaa
    48481 gattggatct gggattggta ccgctgaaag caaggcttga gtatatgggt gaattggatt
    48541 gttaaacaat tcttctgttt ctgcaacctc tacaataaca cccttgtaaa taactgcgat
    48601 acgatctgaa ataaagcgaa caaccgacaa gtcatgggcg atgaagagat aggtcaggcc
    48661 gagctctttt tggaattttt tgagcaagtt caagacttgg gcacgtacag aaacgtccaa
    48721 ggctgaaatt ggctcatctg caataacaaa gtctggttgc atgaccaagg cacgggcaat
    48781 accgatacgt tgacgttgac cgcctgagaa ttcatgaggg taacgagtca agtgctcagc
    48841 aagaagacct acttcacgga taatattttg aactttctct ttacgttctt cttcatcctt
    48901 aaataaacgg tgattgtaaa gaccttcaga aataatataa tcaacagtcg cacgttcatt
    48961 caaacttgcg gcagggtctt ggaaaatcat ctggattcga cgaatcaatt ccgcagcttg
    49021 ttcacgcgat ttcttaccat taatcttttg accatcaaaa atgatatctc cattacttgt
    49081 atcatttaga ccgatgatag cacgaccaat agttgttttc ccactaccgg actcacctac
    49141 aagcgagaaa gtttctccct tgttgataaa gaagttagca tttttaaccg cgacaaactt
    49201 cttacttcct tcaccgaagg aaatttctaa atctttgatt tctactaatt tttcagacat
    49261 ttccttcctc ctagtcagcc agatgggcaa atcccatttt ttcacggatc ttatcatgga
    49321 gatttgcaat cacagctggt ttttctactt ttggagcatc ctcatgaaga agccaagttt
    49381 tagcccaatg tgtctttgat actgagaatt gaggagcttt ttgttcgaag tcaatctgca
    49441 ttgcgtagtc agaacgcaaa gcaaaagcat cccctttcag gtcagtataa agtgacggag
    49501 gtgttcctgg gattgagtaa agatcccctt tatcatcagc aagctgaggc aagctagaca
    49561 agagactcca tgtatatgga tggcgagggt catagaagac ttcctcaacc gttccatact
    49621 caacgatttc tcctgcatac ataaccgcta ccttatccgc aatacttgcc accacaccaa
    49681 ggtcgtgggt aataaagatt gttgtgaaat gatactcgtt ttgtaaagat tttagcaaat
    49741 caataatctg agcttgaata gttacatcca aggcagttgt tggctcatca cagatcaaga
    49801 catcaggtcg gcaggcaagg gcaatcgcaa taacgatacg ttgacgcatt cctccagaat
    49861 attggaatgg gtattcatta aaacgtctat ctgcgtctgg aatgccaacc ttattcatgt
    49921 agtcaatggc caattctttc gcttctttag ctgtttttcc ttggtgtttt acaataactt
    49981 ctgtaatctg actaccaatt gttttaatgg ggtccaaact agtcattggg tcctggaaga
    50041 tagtcgcaat cttagcacca cgaatttgtt cccaatcctt gtgagaagat aaagctgtca
    50101 agtcctgacc acggtagtca atactacctt gggcaatacg accattttct tcgagcatac
    50161 ctgtgaaggt ctttgtcaaa acagatttac ctgatcctga ctcacctacc aaggctaata
    50221 cttctccttc gactagttca agggaaacgc cgcgaatggc tgtcaatact ttgtcacgaa
    50281 cgtcaaattc cacgacaata tcgcgagcag tcaaaattac atttttttct tttgtcattt
    50341 ctactcctat ctatgtgtac gtggatcact agcatccgct aagttttgac caactacgaa
    50401 aagggacaag gataccaaga caagggttgt caatggaatc cagaacaagt aagcattggt
    50461 tgttacgttt tgtgaataat ccgaaatcaa acgacccaaa cttggcactg taatcggtaa
    50521 tccaagaccg aagaaagaca agaaggcttc gtatgagata aagcttggaa gcatttgagt
    50581 catggttgtc acaataacag ataccaattg aggcatgata tttttggcaa caatcttcaa
    50641 ggttggtgtt cccaaagtac gtgacgccaa gttgtattcc aagtcacgat agcgcaagat
    50701 ttgcacacgg atcatgaagg caataccaat ccatgttgtt acgctcatgg caaaaatcag
    50761 attccagaat ccagctccga ttgagtaagt caagacaata acaatcaaaa gaggtgggat
    50821 gtttgagatg acgttgtaaa cttccatcat gacacggtca actgattttg aaatacccca
    50881 aataccaccg acaaaaacac cgataaccaa gttaatcact gtcgcaatca cagaaatgag
    50941 gatggagtta cgagctccga accagacacc gtcaaagagc gatttaccgt tactgtcagt
    51001 accgaaccaa tgctccgcat ttggcttgat ataacgaaca ctaaagtcgt ttaccttgct
    51061 gacatcattg aaatcaaact tagaaaacat tgggtagatg aaacttatca aaatgatggc
    51121 taccaagatt cccaacatga ctacagttga ttttttcttc ataaattgtt taaacactga
    51181 tttccagtaa gaatatgctg gcgcatcaat agtttcagag gcaaaatcgt cacgttttac
    51241 aaactgaaat ttttctttat cgattgtaga cattatttgc ctcctttctc agtcaattta
    51301 atacgtgggt caataatagt catccaaata tctcccaaaa gacgtgagaa gatagaaata
    51361 catgtaaaga tgaagacaag accaacgacc atagagttat tagatgcttt tacagagtca
    51421 atcaacattt tacccatacc tgggaaggcg aagactgttt cagtaagggt tgcaccaccg
    51481 ataaccccaa taatggcacc aggaattcct gaaaccagcg gaaccatggc atttttaaag
    51541 atgtgtttgt ttgaaatttc tttttcagac aaaccttttg cacgagcgaa acgaacaaag
    51601 tcttgagatt gcaagtcaat catgtaacga cgaatccaaa tggctgtacc aggagcaccc
    51661 aacaaaccaa ggatgactgc tggtaaaacg taagaacgcc aatctccagc tcccaagata
    51721 gggaatgaat ctggaagggc aatagatgat ccaatcaatc gaacgatgta aaccaaggca
    51781 atcgttggaa gagcaagcaa gaaggtcaaa gcccctgttg agaggctatc aatccaagtg
    51841 ttcttgaaac gagccatggc tgaaccaagt ggcacggcaa gagcataggc aagaaccaaa
    51901 ccaatcaaac cagtaatagc agagctgaca atcatagatg gatattggta attactttca
    51961 gtcgctgtat aaggatcatc tttcccatag ctagctactt cacgagagtc agcctgacta
    52021 ggtgacttgt aggttcttga gtaaatattt acagaagacg ttttcttacc tgttgggaac
    52081 tgaacttggg cagttttggt ttgtccttga ccttgagtaa taacctgaag aactggtgta
    52141 ttagcatagg ttgggtaaga gtcacctaaa ttcaagttca caaagttttg atgaacaaat
    52201 gggaactgac tgttaaagta caagagatat ttatgtttag ttcctgaacc gaccaatgac
    52261 catccgatag ctggatcatt ttcaaaacga aggtagcgtt tcaagtctgg attttcaggg
    52321 tcttggattt tatttgtatg atcaatgtca atcaagttag catagaagtg aaaaacacgt
    52381 tcaaaaattg gaatttcacg agtagcatag aattgaccac tttcagtaaa ttctcccaaa
    52441 gtccaaccat gacctaattg attgatgtac ttttcataaa tagctttatt ggtcgcattt
    52501 gcttctactg ttacagaaga atccatgcta cttgcctttt cttgcaactc tttagtatcg
    52561 taatactcaa tgtagcccat acgctcaaac acagtatttt catagttatc acgtttatca
    52621 gccgttgtcg caattttatt atagttagga tcctgcttga aaatcaattt tcgaggaacc
    52681 aaggtataga taatcgtgta ggtcaaagtc gttactaaga aaatcgaaac caatgaccgc
    52741 aaaacacgca taaaaatata ttttttcata ttatttcctt taaaaatccc aaaagaacct
    52801 tctcctcatg gagagaaagt tctattagaa attatttact tcacatgact tgccaattct
    52861 ttttgagctt tctcatttga ttcagctttt tctttcaacc atttttcacg agctttttca
    52921 tactcttcct tagtcaccac tttatcttgt aatttcaaat atttgaagta aacatctgac
    52981 cccttagagc ctgtttgcgc agaagctcca gtaaatggaa caattcgtga aagcactggt
    53041 gctgcaccag aagaagccat agcaggaata aagagtgaac tatctgtcaa ccatgcttga
    53101 gccgctgcat atttttcata acggacattc aagtcgcttg tctctctcgc agcttcatca
    53161 actaatttat cgtattcttt caaaccaact tgaactactg aagggctatt tggattatca
    53221 aatcctaaat atgtttttgt agtttcactg ctagttgttt ttaaaatatc caggtaagta
    53281 gatgggtctt gatagtctgg cccccatgaa actcctcccg atacatccca atcctcagat
    53341 gaagcattgg cagcatagta agtaatatta aggaattcat cacttgtcat ttgttgaata
    53401 tcaacaacga cattttcaac accaagaact gtttctacag attgtttaaa ggactgaata
    53461 cgagatatgt agttttttga tgcttggtct actggaacgt ccagatgaat aggaaactga
    53521 acgccgtctg cttctaaagc tttcttagct ttcgcaaact ctgccttggc cttgtcagca
    53581 ttgaataaac catcctgccc atcagctaaa ttcacacctt tccactcatc accataagca
    53641 ggaagttgag cagcgactaa atcaccaaag gtcttctcac cagctgaaac aaagtctggt
    53701 tttacaaata aattacgaac tgctaaagct gctccatctt taccattgat ttgagctgag
    53761 taagctgagc gatcaagagc aaaattcaag gcttgacgga aatctttgtt aagcaatgcc
    53821 ttcttagtag ctactttctc tgaatctgta gttttagaag tatagttgta actttggcga
    53881 tcaatattca cacccagacc agcaatccca gagcctgatt gtgtgtaata gatattgtcc
    53941 ttgtattctt ctgcaacctt agaatagttg gagctggtag ggtaaagacg ggcataacta
    54001 taagctccac tagtgaagtt acgctctagc gactcctgat ctgatccatc atagtaagct
    54061 agattgatag tatctaggtg gacattttct ttatcccaat attgctcatt ttttacaaac
    54121 tctacagaag attttgcagt caaccctttc aacaagaatg gaccattata aagcaaggat
    54181 gtcggatctg ttggtttagc aaaatcgctt ccttttgatg tttcgaattc ttcattcaga
    54241 ggccagaaaa tagaataggt caacttagag ttccagaacg gttcaggctg gttcaaagtg
    54301 tattgtaacg tataatcatc aaccgccttg acaccaactg ttgaaaaatc tgttgaagtt
    54361 cctgatagat aatctgccaa gcctttaacc gaattttcag ctaaatacat agcttctgat
    54421 tttttatctg ctgcgtgttt taaaccgttc acgaaatctt tagccgtcac ctctgcatat
    54481 tcttctccat cagaggtaaa ccatttaacc cctttacgaa tcttataagt gtaggtcaaa
    54541 ccatccttag agacttccca atcctctgca actgcaggag caagattacc gtaattatcg
    54601 ttagtgaata aaccatcaat cccatttgaa gtcactactg ttgtactatt tttacttgaa
    54661 atcaggtagt ccaaggtttc tgggtctgct gtataaacat agccataagc tttaggggct
    54721 gatgaatcag atgattttga agaactgcat gctgcaagta cacctgctgc taataaaaca
    54781 agacctgctg tagcaaatac acgatttttt ttcattttct actcctctgt ttatgtgaat
    54841 tatagattga caaccattat atcacattat ccattaaaaa tcaaacaaat tttcagaata
    54901 tttaggcttg ttggcacaaa tttttcattt tttttgaata tatgattcaa attgtcgttc
    54961 gaagtgtcaa agactacagt gaaaatagga aatttgacgc agaaactttg gagtttagga
    55021 agacatacag taaaatgaaa tacggacgga acaatgtgat tttggaattc aaattaaatt
    55081 ataacaatat tgtagaagta tcattctagt attcaagatt cagtttacta tgtcttttca
    55141 caccaacctt attccgaatt caattacttt tgtgatttac atatatagat taagactatc
    55201 ttttatactt taaaatttct cgctacctta tccactatat gctcctcgct atcacgtttc
    55261 tattcatagc ctacgatttc actattgctt tctctgacaa ttcttatttc ctgcgtcaga
    55321 cttaaaacga tctatcccca gaccatttta atccgctacc tcacgacagt caggcttggg
    55381 gagcgctatt gtattcaccg gtagtggatc cctacagagg acttacacct cagatgcacg
    55441 acatgcccat agtataaaaa aatctcctac ccaaggtaga agatttcaaa cttataaaac
    55501 ttaatccgtc atgtccgata ccaacattcg atgctccaat ggaatactgc acataactag
    55561 caagaaaata aagcctgact gaatccagaa gagagccaag tcaaaaattc cgtgcacagc
    55621 aaccactgta aggaaagata gataaaggcc gataatcgga cgtttccccg actcctgact
    55681 catatccatc atcaagcgaa caggagcaac agaagacaaa actaataaaa tggtacccac
    55741 aattccgtaa ctcagaatcg tatcaatata aagactgtgg gcatgttcat gataaggagc
    55801 atgtatccga ggataagagt gcatataggt caatggccct tcaccccaaa aaggattttg
    55861 cttaaacaag gccatcccag catcccagat agaaatgcgt tcttccatag aagagtctaa
    55921 agtacccatt cgaactccca aatcactaga aaagaggaaa ctcaaaccaa tcgcgaagac
    55981 cccaatacta agccaaaagg ccttccagtt tttaatagtc gtaaagagat agataattgc
    56041 tccagcgata atagcaggaa aggcagttcg attttgagta aagttcaaac caaagagatt
    56101 aacaaagcct gcaatcacac agaatacttt caaccaattc aacttggtcg ttgtaaacag
    56161 atagaaagca atcataatac agaaacaaca aataattcca taataattag gattaaagaa
    56221 ggtcacttct gcccggttct gatgccacac ctgcatattg ggtgaaagaa aagcatagtt
    56281 aaatttcttc acaatttgga aatgttctaa actggcaaaa gcagctgaca agacactacc
    56341 aaacaagacg aactgcaaaa tcaatcgaaa gaatttatgg gataaaatcg actgatagtg
    56401 caaaaagaaa atagtaaata gaaacattcc tactgaagcc acaagaccca tccaattttg
    56461 tgcaagaatg gatataacag tactatagct aagaaaaaga agcagcatcg gatgctcccc
    56521 cattttctga agaatacttt tcatgtctcc tgtaaaaatc aaactgataa tatataaaca
    56581 gagtacaact acaaaaagat aaaagggtaa aaagatactt aggataattc ccaataaaat
    56641 cagctcttta ctagacaacc ccttcagctt ttcaataaag cctattgatt tcaaaatgaa
    56701 tcctttctct ccaaatcagc tgattcagat aatagtaagc tatcctatat tgtaccactt
    56761 ttttagcaat ttgaaaacaa aggaaacgtt ttccaaaata aaaaccctat tttatccacc
    56821 atatcaaggc ttcaaaatgt tacttcaact ccattctcaa ttacccgata agtctgattt
    56881 tgcaaatcaa tttctactac tgctgttacg gacttatctt tattttgacg tttgattaca
    56941 atgctgtgag ctgttggtgt ctctatctca gtagtccctt ctagatcaaa ggcttctgaa
    57001 cggttacgga aagaaaatag attgagaagg gccttcacaa caggtcgttg cacttctttt
    57061 gctatttctt cgttgctata gtaatgacga ttaatatttc gaccttcttt agtttcttct
    57121 aataatttca agtcattctt gcctgctaat agacccacat agtaaacctg aggaatacct
    57181 ggggcaaaag cttgaattag acgagcgaga aaatacttga catcatcatc tccaagcgct
    57241 gaatagtagg ttgaattgat ttggtagata tctaagttgt tatactcggc actagagtac
    57301 ttacgtttga cattggctcc aaccttatag agttcatttg aagcatagtc aatctcctca
    57361 tcggtcagga tatccttgac atctactact ccaatcccat catgggtatc tagcgtcgta
    57421 aattgcttca tcgggctcat ctttaaccac ttagccaaac gctctgttct ggaactgtaa
    57481 agagtataaa gtgtcaccat tggaagagca aaatcataaa catagtaatc atggtctgct
    57541 attttaaact gaatcgaata gtgttcatga atctcaggta aaagctctgt cccatactca
    57601 gcagcgatat ctcgaacttt gtccaataaa tcccaaatat ctggttccac aaagaaatca
    57661 ttagtatcca atttcttcac tgcataagca aaggcatcta gacgaatcaa atcacaccca
    57721 ttacttgcca agtgctgaat ggtcttacgg ataaattcca tagttacttc tttggtcaca
    57781 tcaagatcaa tctgctcctc accaaaggta ttccacaaat gttccactga accatcttca
    57841 aacacaatct cttgctttgg tgcacgatcc ttacgcttgt aaattaaatc tacatcagac
    57901 tgtgtcggac ggttttctgg ccaaaactta tcccagttta aaaagagatc tttaaattca
    57961 ctggcttcat gtttttcttg atagtcctta taatacttgg attgacgaga aatatgatta
    58021 atcataaaat caaacataag ataatatctc tcacctaaac gcttaacatc ctcccaatca
    58081 ccaaaagctg agtccacttc gtcgtagtca actggcgcaa atccacgatc acctgttgat
    58141 gggaaaaatg gtaaaaggtg aactcctcca atagcatctc caaaatgctc ttccaaatta
    58201 tcatataagt ctttaagatt atttccaagg ctatcagaat aggtaatcaa catggtttta
    58261 ttttgaattg gcatcattac tctccttttt ctaattgaag ccaagtctca tatgatctgg
    58321 cttcataaat aaaattcatt ttaaatctct atttatcatc aaactcgtac taatatagac
    58381 tgtgataaac aaagtactac tttcttgttt tctacataga attatcaaca agctgaactc
    58441 ttcctctgtg tcaaagacta tagattccat gagctcttct tatactattc gaaaatctct
    58501 tcaaaccacg tcagcgttgc cttaccgtag gtatggttac tgacttcgtc agtttcatcc
    58561 acaacctcaa aacagtgttt tgagcaacct gcggctagct tcctagtttg ctctttgatt
    58621 ttcattgagt attacttcac tgccccgttg cttattcctg cgatgatatg gcgttggaag
    58681 aagagataga caatggtgat actgataatg ccgaccacgt aagaggcaaa gcttggtccg
    58741 tagtcgttga aatattggcc tgcgtagttg tattggaaca aaggcagagt ccacattttg
    58801 gaatcccggt tcaagacaag gagtggcaac atgaagtcat tccagaacca aagggcattg
    58861 atgatcatgg ttgtcgcatg catcggtttc atcattggga agatgatgcg gaaataggtt
    58921 gtaaattgat tagccccatc gatctctgct gcttcatcca gactttctgg aatcgagatt
    58981 ttgatatagc caacatagag aaagagggtc tgtggaatcg cataggtcaa gtagagcaag
    59041 atcaaaccaa aggtattagc caaaccgagt ttactcatca taaccgtaat cggaatcatg
    59101 atgacttgga aaggtacgaa gattccgagg attaagaggg tatacatgat ggtaaaggct
    59161 tttcttttac tcatattgcg agcgatggag taggctgcca tagggataaa gatcattact
    59221 gcaagtaaag acaagacagt gatgacgaca gagttccaat aatagcctcc aatcccatca
    59281 gctaagagac ggctaaagtt gtcccatgtg aagttggttg gaaagccaaa gaaattatct
    59341 acaatatcct tagtgggttt gaaggaacta aagagggtag caaggagcgg cactaaaatc
    59401 agaaccgatc ctagaatcaa tagaatgtat ttgccaatca gggcttttct ttcatcttgt
    59461 ttcatcatgc ttctcctctt aaatttcaaa tttcttagat actctcaatt ggatgatcga
    59521 aatcactaca attaagaaga acaagattac ggcaatggca ttggcataac cgaattggtt
    59581 gtttttaaag gcatagttat aaaccaagag cccaagtgag gttgtggcat tgtttggacc
    59641 accaccggtc atggcaaaga cttggtcaaa ggcagtcagc ccacctttta gggctaggat
    59701 aaagactata gagacacttg gtagcaagta aggcaattca atgttccaga aaacttgctt
    59761 gctagtcgca ccatcaatcc ttgctgcctc tgtaatctca gttggaatag attgcaaacc
    59821 agctaggaag atgatgatgg gcatagccac cccttgccaa agaaggacaa agacagccgc
    59881 aaagattgct ccccacttag tccctaaaag actggtttgg agaaattcaa tatgaagggc
    59941 attcccaatc gctggaagac cgtagttgaa gacttgcttg aagatcaaag ccactgtcaa
    60001 accagataaa acagctggga agaagaacca agcacggaag aaggtttggc ctttgatttt
    60061 agagttcaag acacgcgcaa tgaagatccc gagtgcaatc tcaccaacca ccatggcaat
    60121 cgtaatgatt gcggtaaagc caatcgcatt catgaatttt ggatccatga agaggagctt
    60181 aaagttgttt aagccgacaa atttgtagtt gtaagtcaat cctgtccagt tggtaaaact
    60241 gtaaaaggct ccttgaaaca tcggcacata gaagaaaatt gcttgtaaca agagggggat
    60301 gaccacaaaa gcccatgccc aatatttttg taatactttt ttcatagtct ctctactcct
    60361 aatccacatc cgctttcatc gggttaaaga aggcattcaa atcattgacc atgccttgtt
    60421 tatcaccggt caagacatag ttcatggtca aggtatggaa gtctgcttca ctggtccagt
    60481 attgttgcaa ccagaccaag tgacgatccg taaaggcata ttcggtcata ccagcaagcg
    60541 gtgaatcttc tcctgcttgt ttgacccctt cgatcgctgt tggagatccg tccacatcgt
    60601 agtatttttg catgacttct ggacgggtca tatattccac aaagacattg gcttcttttg
    60661 gatgtttggt ggtggctgag atagaccatg ccaagtctcc cgcaccaacg gttaagcttt
    60721 gtcctttttc ttttcctgga atcatgaagg tcccaatctt aaagttcggt ttttgttcat
    60781 taatcgctgt gatcgcccaa gacccatttg gtgtcatgag gacatcccca cgtgcgaagg
    60841 ctccgataac atcggtatag ccagcacctt cccagttctt ttgcttagag ccattgatgc
    60901 gaaggatgtc catgaccttg atatcatctt tcataatcgg atccgacaat ttaatggcat
    60961 ttggttgaga ataacgaagg tattgatttg cttcttttcc tccacctgtt gctgtcgcaa
    61021 aggctaattg attgtaacca ttgagtgtcc aagcatctgc acctgcaatt ccaaatggtg
    61081 tttgtccttt agcaacgata tctttgacta actgttcaaa ttcatcccag gtttcaggaa
    61141 ccttcaagcc cagttcttcg aatttatctt tgttgtagta aattccataa gcattagctg
    61201 taaaaggaac gttgtaaact ttttcgttta cagcatattt ttcagcgtag ccatttttca
    61261 cgcgtttcag gtagtctttg ttgctcaaat cttcaaagac acctgctttt gcccattctt
    61321 gcagttcgat ggattgtggg taaatattga ccacatcagg cacatctcct gcgagaacgc
    61381 gtgtcttcaa tacttcacca gcatttggta cattgacgac tttgaccttg atcttagggt
    61441 tttccttctc aaaatcacga gtgatttctt ccaaggtttt ggtcatttct tttttctggt
    61501 tgaaatactc gatggtcact gtgtcatccg cagatttacc atagttggag caagcgccga
    61561 gcccaaacaa agctaaacct gtagttgcaa gaagtccgat ttttttatac cattccatta
    61621 gaaagcctcc tttataaatt tatacaccct tatcatagtc ttctaaaaat atactgtcta
    61681 ctcaaaaaat ctccttggga taagataaca gttaggcccg catacattag ttctgcacct
    61741 gagtaaactt cgccattttc ctgtaattca tatagtccct cttcatccaa atcttttaat
    61801 tttaaagttg tttccatggt ctctacaaca gataaaacgc gaacgtaggt tacaatcgtt
    61861 tgatttccgt aattaaattg tacagctgct tcattggatg cagtatcagg attaattagt
    61921 ctatactgct gtcctaactg aactactggt cgtaattctt tatacaagtt cacctgatta
    61981 gcaatcgtag ctttctcttc atctgataaa tttgtcaaat caagctcata gcccaaattt
    62041 cccatcattg ctacatggcc acgtgtttct aatggtgtca ttcgtcccat ctgatgattc
    62101 ggtactgctg acacatgagc ccccatagaa atggttggat agagatagga tgaaccgtat
    62161 tgaattggta aacgtgcaat ggcatcagta ttatcactag cccagacttg tgggaaatag
    62221 cgtatcatac caagatcatt tcgtccacca ccaccagagc aggactcaaa gagaatatgg
    62281 ctgtgcttct ctgtcagata agaaacgagt tcgtaaagcc ccagcatgta ctgatgagat
    62341 tgcatctgtg tctctagata agttaatcca ttccctagct tagtgatatt gcggttcata
    62401 tcccatttaa tgtaatcaat atcatgataa gataggagtt gatctaagac acttttcaag
    62461 tattctacta cctgaggatt ggcaagatta agtactaatt gattccgaga ataagtatgc
    62521 tcatagtcag gaacctgaat agcccagtca ggatgtcgac gatacaaatc actatctaca
    62581 gaaatcattt cgggttctaa ccaaagtcca aactgcaaac ctctttcatg gatagctgaa
    62641 atcagacttt ctagacttcc acccagtttt tcctcattaa caacccaatc acctaaagca
    62701 cgattatcat caaaacgatt gccaaaccaa ccatcatcta atacaaaaag ttcaatgcca
    62761 actttcttag cttcatctgc taactctaac agtttttctc tctgaaagtc aaagtaagta
    62821 gcttcccagt tattgattag aattggacgt tcttttttag aaaattcact tagcataatg
    62881 tgcttcagta caaaattctg actttcatga ctaataccag ttaatccctg atctgaatgg
    62941 gtcactaaag ctactggtgt ttcaaagtat tcctcaggag ctaacttcca agaaaagttt
    63001 tctggattaa tgccaatagc cacccgaact tcattcaatt gatttttttg aacaaaagct
    63061 tcaaagttgc cactatacat tagttgaata gcaaacacat tcccagcatc ctctgtgact
    63121 ccttgttcgc atagtagaag agctggtgtt tgagtatgac cagaagcacc tcggtttgag
    63181 ctaatcgaaa agattccttg ttctacctgt tgacgtctaa cagtcttttc acgagcataa
    63241 gcaccctgca gagttactat ttcgtaatct gcagctggaa aatcagccat aaaagaaaaa
    63301 tctttatgga tgacaacttc ctgattacta ttattatcta atttactgta gctagcaata
    63361 gtcgcatcat tattaaaagt agtataatac aaagtcagac taagttgagc cttagaatct
    63421 tctaacatta agacaagagt ctctgtatcg tccatgctat gtggagaagg taagccctgt
    63481 ggaccattct gaccttttaa aatctttgct tctacaaatc gaaagtctgt tacttcagtt
    63541 acactatgct gaacctgtat ggttggtttc ctaaaatctc ctaagccatg ttgtccaaaa
    63601 atctgtcgct gagtatctaa actaaaggtt cgattagtag ccgttggatt tcctgaaaag
    63661 gcatggtctc gttcataaac actattggaa cctttatagt tcttaatagt ctttcctaaa
    63721 tgtttcaaaa gtaagtagcc atttcgattt tcaataatca aacttagatt tttactctca
    63781 acataaaata gattattctc tatcctaact cccatttact tcacctcatc actttattga
    63841 ttatatttta tcacctgaaa tcgctttcca aaatagaaaa atgtctcaag aatatggtaa
    63901 aatgttaggt aggaggtagc acatgctagt tttttcagaa taccagactg gaacaattga
    63961 ccttgcccta agcttttatg gatatgagga atgcacacct aattactctt ttggtccagc
    64021 cattcgtgat acatacgtcc tacattacat tactaaagga caaggaaaat ttcattacaa
    64081 gggtaaaatt gttgatttaa aagaaggaga tttctttcta ttaaaaccag aggaactaac
    64141 cttttatcaa gcagatagta aagaaccttg ggcctactac tggttaggaa tcactggagg
    64201 gaaatcccct gattattttg ctctttctca aatttctgat caatcctatc tcatccaatc
    64261 tgaaacttgt catacccaga ctactgcaaa actcatctca gacattgtcc gcttcgctca
    64321 gattacaaaa tcaagtgaat tatctcaact ccatatcatg ggacaacttc atgaactgat
    64381 gtttcatctg ggaactattg ctcccaatca gaaaaaaaag aatatttcat caacccacca
    64441 actctatctt gaatgcaaac gattaattga tagccactat cctcaatcac ttacaattca
    64501 agatttagca aaagaactat ccgttcacag aagctactta tcaagcgtat tcaaagaatt
    64561 taatacctta tcacccaaag aatacctact ctacattcga atgcaccgag ctagacaact
    64621 tctcgaaaat acccaagagt ccatcaaggt aattgcatac tcggtaggtt tttcagatcc
    64681 actccatttt tcgaaagctt ataaacaata ctttaatcag actccaagtc atacaagaaa
    64741 agaatactct caataccaac tagtgagaaa ggcaacatta tgaaatccta ccaagctgtc
    64801 taccaaatcc tatctaaaga aaccgactat atcagcggag aaaaaatcgc agaaaaacta
    64861 tccctaagcc gaacatcaat ttggaaagcc atcaagcgac tagaacaaga aggcattgaa
    64921 attgatagta tcaaaaatag aggatataaa ttgatgaatg gtgaccttat tcttccagag
    64981 attctagaag aaaatcttcc aattaaagtc agctttaaac ccgaaacaaa atcaacacaa
    65041 ctagatgcaa aagaagcaat tgatttaggc catgaagcaa ataccctcta tctagcttcc
    65101 tatcaaacag caggccgagg ccgttttcaa cgttccttct actcaccaca aggtggtatt
    65161 tatatgacac tccatcttaa accaaatctc ccctatgaca aattaccatc ctacacacta
    65221 cttgtagctg gagctgtcta caaagctatt aagaacctaa ctttaataga tgtcgacata
    65281 aaatgggtca atgatatcta tctaaacaat cataaaattg gaggaatcct tactgaagca
    65341 atgacctctg tagaaactgg cttagtcaca gatatcatta ttggagtagg tatcaatttc
    65401 actattaaag acttccctca ggaattaaaa gaaaaagctg ccagcttatt taaagctaca
    65461 gctcctataa caaggaatga attgatcata gaaatctggc gtgctttctt cgaaacacca
    65521 gcagaagagc tattatacct atacaaaaaa cagtcattca ttctaggaaa agaagtcact
    65581 ttcacactag agcaaaaaga ctacaaggga cttgctaaag acatctcaga aaatggaaaa
    65641 cttttaattc aatgtgataa cggaaaagaa atctggctaa atagtggcga aatttctctc
    65701 aatagttgga agtaaaataa cacaattata atataaacga tataaaaata acttcagatt
    65761 agtaattcaa ttaagtttta cggatctgaa gttttattgt ctctaaaaac aaaaaagaga
    65821 gttacaaact ctcattaaaa cggagaataa gggattcgaa cccttgcgcc agttacccga
    65881 cctaacgatt tagcaaaccg tcctcttcag cctcttgagt aattctccaa ttaatgggca
    65941 cgagtggact cgaaccaccg acctcacgct tatcaggcgt gcgctctaac cacctgagct
    66001 acgcgcccaa gttaaaaaac ttggtaattt gaacaaagtt caaagcgggt gacgagaatc
    66061 gaactcgcga caacagcttg gaaggctgta gttttaccac taaactacac ccgcataaat
    66121 actatcaata aaatggcgcg agacggaatc gaaccgccga cacatggagc ttcaatccat
    66181 tgctctacca actgagctac cgagccttat tgcgggagca ggatttgaac ctacgacctt
    66241 cgggttatga gcccgacgag ctaccgagct gctccatccc gcgttaataa tataaaagga
    66301 ggatgtggga ttcgaaccca cgcacgcttt tacacgcctg acggttttca agaccgttcc
    66361 cttcagccgg acttgggtaa tcctccaata ttcaaatgga ccttgtagga cttgaaccta
    66421 cgaccactcg gttatgagcc gagagctcta accagctgag ctaaaggtcc gacaagatca
    66481 ttatagcggc gaaggggatc gaacccccga cctcccgggt atgaaccgga cgctctagcc
    66541 agctgagcta caccgccatg aatcgggaag acaggattcg aacctgcgac accttggtcc
    66601 caaaccaagt actctaccaa gctgagctac ttcccgagtt aaatagaaaa atgcacccta
    66661 gaggagtcga acctctaacc gcctgattcg tagtcaggta ctctatccag ttgagctaag
    66721 ggtgctccat attatgccga ggaccggaat cgaaccggta cgatcgttac caatcgcagg
    66781 attttaagtc ctgtgcgtct gccagttccg ccaccccggc ctctctaagc gaacgacggg
    66841 attcgaaccc gcgaccccca ccttggcaag gtggtgttct accactgaac tacgttcgca
    66901 ctgttttctt ctatctaaaa atgccggcta catgacttga acacgcgacc ctctgattac
    66961 aaatcagatg ctctaccaac tgagctaagc cggctcattt gttatatctt aatgcgggtt
    67021 aagggacttg aacccccacg ccgttaagcg ccagatccta aatctggtgc gtctgccaat
    67081 tccgccaaac ccgcatatat gacccgtact gggctcgaac cagtgaccca ttgattaaaa
    67141 gtcaattgct ctaccaactg agctaacgag tctaaaataa cttgcgttac cttaaacggt
    67201 cccgacggga atcgaacccg cgatcttcgc cgtgacaggg cgacgtgata accgctacac
    67261 tacgggacct ttgggagtta acgggatcga accgctgacc ctctgcttgt aaggcagatg
    67321 ctctcccagc tgagctaaac tcccta
//
LOCUS       DAVKAO010000004        66952 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKAO010000004 DAVKAO010000000
VERSION     DAVKAO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.004"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(430..2157)
                     /gene="ezrA"
                     /locus_tag="VWX16_000253"
     CDS             complement(430..2157)
                     /gene="ezrA"
                     /locus_tag="VWX16_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9157673.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHASDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2239..4185)
                     /gene="gyrB"
                     /locus_tag="VWX16_000254"
     CDS             complement(2239..4185)
                     /gene="gyrB"
                     /locus_tag="VWX16_000254"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9157674.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAE
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLAAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4200..4763)
                     /locus_tag="VWX16_000255"
     CDS             complement(4200..4763)
                     /locus_tag="VWX16_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9157675.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4848..5402)
                     /locus_tag="VWX16_000256"
     CDS             complement(4848..5402)
                     /locus_tag="VWX16_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9157676.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5413..6636)
                     /locus_tag="VWX16_000257"
     CDS             complement(5413..6636)
                     /locus_tag="VWX16_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9157677.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6673..9123)
                     /locus_tag="VWX16_000258"
     CDS             complement(6673..9123)
                     /locus_tag="VWX16_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9157678.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SHSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9323..10252)
                     /locus_tag="VWX16_000259"
     CDS             complement(9323..10252)
                     /locus_tag="VWX16_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9157679.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10392..10679
                     /locus_tag="VWX16_000260"
     CDS             10392..10679
                     /locus_tag="VWX16_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9157680.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10713..12047)
                     /gene="ciaH"
                     /locus_tag="VWX16_000261"
     CDS             complement(10713..12047)
                     /gene="ciaH"
                     /locus_tag="VWX16_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9157681.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12037..12711)
                     /gene="ciaR"
                     /locus_tag="VWX16_000262"
     CDS             complement(12037..12711)
                     /gene="ciaR"
                     /locus_tag="VWX16_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9157682.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12820..15366)
                     /locus_tag="VWX16_000263"
     CDS             complement(12820..15366)
                     /locus_tag="VWX16_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9157683.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15489..15929)
                     /locus_tag="VWX16_000264"
     CDS             complement(15489..15929)
                     /locus_tag="VWX16_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9157684.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(15904..16773)
                     /locus_tag="VWX16_000265"
     CDS             complement(15904..16773)
                     /locus_tag="VWX16_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9157685.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16815..17543)
                     /locus_tag="VWX16_000266"
     CDS             complement(16815..17543)
                     /locus_tag="VWX16_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9157686.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17551..18030)
                     /locus_tag="VWX16_000267"
     CDS             complement(17551..18030)
                     /locus_tag="VWX16_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9157687.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            complement(18041..18739)
                     /locus_tag="VWX16_000268"
     CDS             complement(18041..18739)
                     /locus_tag="VWX16_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9157688.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18732..18998)
                     /locus_tag="VWX16_000269"
     CDS             complement(18732..18998)
                     /locus_tag="VWX16_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9157689.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(19064..19990)
                     /locus_tag="VWX16_000270"
     CDS             complement(19064..19990)
                     /locus_tag="VWX16_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9157690.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSIIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20065..20880)
                     /locus_tag="VWX16_000271"
     CDS             complement(20065..20880)
                     /locus_tag="VWX16_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157691.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20884..21204)
                     /locus_tag="VWX16_000272"
     CDS             complement(20884..21204)
                     /locus_tag="VWX16_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9157692.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(21580..23577)
                     /gene="metG"
                     /locus_tag="VWX16_000273"
     CDS             complement(21580..23577)
                     /gene="metG"
                     /locus_tag="VWX16_000273"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9157693.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEKSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(23701..24960)
                     /locus_tag="VWX16_000274"
     CDS             complement(23701..24960)
                     /locus_tag="VWX16_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9157694.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENTNLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24962..25663)
                     /locus_tag="VWX16_000275"
     CDS             complement(24962..25663)
                     /locus_tag="VWX16_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157695.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELIERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(25647..26846)
                     /locus_tag="VWX16_000276"
                     /pseudo
     CDS             complement(25647..26846)
                     /locus_tag="VWX16_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            27132..28478
                     /gene="gor"
                     /locus_tag="VWX16_000277"
     CDS             27132..28478
                     /gene="gor"
                     /locus_tag="VWX16_000277"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9157696.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERDFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            28675..29211
                     /locus_tag="VWX16_000278"
     CDS             28675..29211
                     /locus_tag="VWX16_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9157697.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAILSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            29262..29348
                     /locus_tag="VWX16_000279"
                     /pseudo
     CDS             29262..29348
                     /locus_tag="VWX16_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(29417..29632)
                     /locus_tag="VWX16_000280"
     CDS             complement(29417..29632)
                     /locus_tag="VWX16_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9157698.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            29730..30050
                     /locus_tag="VWX16_000281"
     CDS             29730..30050
                     /locus_tag="VWX16_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157699.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(30080..30418)
                     /locus_tag="VWX16_000282"
     CDS             complement(30080..30418)
                     /locus_tag="VWX16_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157700.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(30430..31149)
                     /gene="trmD"
                     /locus_tag="VWX16_000283"
     CDS             complement(30430..31149)
                     /gene="trmD"
                     /locus_tag="VWX16_000283"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9157701.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFNSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(31139..31657)
                     /gene="rimM"
                     /locus_tag="VWX16_000284"
     CDS             complement(31139..31657)
                     /gene="rimM"
                     /locus_tag="VWX16_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9157702.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(31688..32476)
                     /locus_tag="VWX16_000285"
     CDS             complement(31688..32476)
                     /locus_tag="VWX16_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9157703.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGRNHHYLQWSESEKIAAIIRELSE"
     gene            complement(32580..32823)
                     /locus_tag="VWX16_000286"
                     /pseudo
     CDS             complement(32580..32823)
                     /locus_tag="VWX16_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(33053..33292)
                     /locus_tag="VWX16_000287"
     CDS             complement(33053..33292)
                     /locus_tag="VWX16_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9157704.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(33312..33584)
                     /gene="rpsP"
                     /locus_tag="VWX16_000288"
     CDS             complement(33312..33584)
                     /gene="rpsP"
                     /locus_tag="VWX16_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9157705.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            33763..33963
                     /locus_tag="VWX16_000289"
     CDS             33763..33963
                     /locus_tag="VWX16_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157706.1"
                     /translation="MNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMNLLAD
                     DSLAGDIILLWRIQFGTFTTET"
     gene            34442..34663
                     /locus_tag="VWX16_000290"
     CDS             34442..34663
                     /locus_tag="VWX16_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157707.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            34746..35549
                     /locus_tag="VWX16_000291"
     CDS             34746..35549
                     /locus_tag="VWX16_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9157708.1"
                     /translation="MKKLATLLLLSTVALAGCSNVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILNTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(35605..37146)
                     /locus_tag="VWX16_000292"
     CDS             complement(35605..37146)
                     /locus_tag="VWX16_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9157709.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHDPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(37148..37630)
                     /locus_tag="VWX16_000293"
     CDS             complement(37148..37630)
                     /locus_tag="VWX16_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9157710.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLSWLLYKRLHKNKIRN
                     "
     gene            complement(37630..38715)
                     /gene="rlmN"
                     /locus_tag="VWX16_000294"
     CDS             complement(37630..38715)
                     /gene="rlmN"
                     /locus_tag="VWX16_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9157711.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIEIVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVNHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGT
                     DIDAACGQLRFNTMKRDRQKVVAAVNP"
     gene            complement(38736..39266)
                     /locus_tag="VWX16_000295"
     CDS             complement(38736..39266)
                     /locus_tag="VWX16_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9157712.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            complement(39423..40028)
                     /locus_tag="VWX16_000296"
     CDS             complement(39423..40028)
                     /locus_tag="VWX16_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9157713.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(40201..41238)
                     /locus_tag="VWX16_000297"
     CDS             complement(40201..41238)
                     /locus_tag="VWX16_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9157714.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(41272..42207)
                     /locus_tag="VWX16_000298"
     CDS             complement(41272..42207)
                     /locus_tag="VWX16_000298"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9157715.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(42760..43950)
                     /locus_tag="VWX16_000299"
     CDS             complement(42760..43950)
                     /locus_tag="VWX16_000299"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9157716.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLIRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(44165..45508)
                     /locus_tag="VWX16_000300"
     CDS             complement(44165..45508)
                     /locus_tag="VWX16_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9157717.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(45648..46463)
                     /locus_tag="VWX16_000301"
     CDS             complement(45648..46463)
                     /locus_tag="VWX16_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9157718.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            complement(46515..48695)
                     /locus_tag="VWX16_000302"
     CDS             complement(46515..48695)
                     /locus_tag="VWX16_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9157719.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEV
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     NAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            complement(48981..49907)
                     /locus_tag="VWX16_000303"
     CDS             complement(48981..49907)
                     /locus_tag="VWX16_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9157720.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            complement(49900..50592)
                     /gene="ftsE"
                     /locus_tag="VWX16_000304"
     CDS             complement(49900..50592)
                     /gene="ftsE"
                     /locus_tag="VWX16_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9157721.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            complement(50610..51705)
                     /gene="prfB"
                     /locus_tag="VWX16_000305"
     CDS             complement(join(50610..51632,51634..51705))
                     /gene="prfB"
                     /locus_tag="VWX16_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9157722.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(51813..52469)
                     /locus_tag="VWX16_000306"
     CDS             complement(51813..52469)
                     /locus_tag="VWX16_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9157723.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            complement(52777..53487)
                     /locus_tag="VWX16_000307"
     CDS             complement(52777..53487)
                     /locus_tag="VWX16_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157724.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            complement(53487..54251)
                     /locus_tag="VWX16_000308"
     CDS             complement(53487..54251)
                     /locus_tag="VWX16_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157725.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            complement(54251..55207)
                     /locus_tag="VWX16_000309"
     CDS             complement(54251..55207)
                     /locus_tag="VWX16_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9157726.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            complement(55211..56080)
                     /locus_tag="VWX16_000310"
     CDS             complement(55211..56080)
                     /locus_tag="VWX16_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9157727.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            complement(56348..57508)
                     /locus_tag="VWX16_000311"
     CDS             complement(56348..57508)
                     /locus_tag="VWX16_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9157728.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(57610..57858)
                     /locus_tag="VWX16_000312"
     CDS             complement(57610..57858)
                     /locus_tag="VWX16_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9157729.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(57937..58527)
                     /gene="clpP"
                     /locus_tag="VWX16_000313"
     CDS             complement(57937..58527)
                     /gene="clpP"
                     /locus_tag="VWX16_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9157730.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(58701..59330)
                     /gene="upp"
                     /locus_tag="VWX16_000314"
     CDS             complement(58701..59330)
                     /gene="upp"
                     /locus_tag="VWX16_000314"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9157731.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLNLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(59419..59886)
                     /locus_tag="VWX16_000315"
     CDS             complement(59419..59886)
                     /locus_tag="VWX16_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9157732.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(59905..60462)
                     /locus_tag="VWX16_000316"
     CDS             complement(59905..60462)
                     /locus_tag="VWX16_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9157733.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            60587..61432
                     /locus_tag="VWX16_000317"
     CDS             60587..61432
                     /locus_tag="VWX16_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9157734.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            61491..62666
                     /locus_tag="VWX16_000318"
     CDS             61491..62666
                     /locus_tag="VWX16_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9157735.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            62686..63141
                     /locus_tag="VWX16_000319"
     CDS             62686..63141
                     /locus_tag="VWX16_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9157736.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(63161..63915)
                     /locus_tag="VWX16_000320"
                     /pseudo
     CDS             complement(63161..63915)
                     /locus_tag="VWX16_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            64121..65456
                     /locus_tag="VWX16_000321"
                     /pseudo
     CDS             64121..65456
                     /locus_tag="VWX16_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(65496..66440)
                     /gene="manA"
                     /locus_tag="VWX16_000322"
     CDS             complement(65496..66440)
                     /gene="manA"
                     /locus_tag="VWX16_000322"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9157737.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(66561..66857)
                     /locus_tag="VWX16_000323"
     CDS             complement(66561..66857)
                     /locus_tag="VWX16_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9157738.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGASIG"
BASE COUNT        19702 a        14606 c        12208 g        20436 t
ORIGIN      
        1 aggttttaga ggcctattta ccgtgggact aaagttgtac aagaaaagtg caaataagaa
       61 atctccagat taggaactat ccgtgagttc tctagtctgg agatttttca atagacttcg
      121 ttattggacg gttaccaagt tactcaaagt acagatttga agctgcagat aagactgacg
      181 aaatcacttt aatactaaat gaaaatcaaa aagcaaacta agaagctagc ctcaggctgt
      241 acttgagtac ggtaaggcga cgctgacgtg atttgaagag attttcgaag agtataaaac
      301 actgttttga ggtggtggat aaatcggctg ggtaaaaact caatctcagg agaaaatgaa
      361 gtaaatctcc ccacaactat agaaaaagat ttgattctgc tcctcacaca ataaaatctt
      421 tttcttttat taaaaacgaa tcgtttcacg tgttttctca tatgaggtaa caaagcgatt
      481 ggttacacca ggctctgcca cttccaatgc ttgagaaatc ttgtcaaatg aagcgtgata
      541 atcaaattct ttttcaaaaa tatctaaagc ttcgttaaat gcttcttgaa tgcgttcatc
      601 aaatgagcga tagcggttag aatattgcaa gagttgctct gtcaaagttg catattgtac
      661 aatattataa gtttccgttt ctaaagcttc catatcattc gttgcaattt caagaactcg
      721 ggtaacagat tcaatgttaa tcattttttg ttctaactca accattaaat cctcggtatt
      781 attgcttgcc gtaaagaata acttcaagaa agtttgtgga atacctggca gattgcgttt
      841 ttccatgtat cgcttgatag tatggagacg attgacataa acattggcct tttgacgtgc
      901 attaatatca tctttctcaa tttgtgtcag gcgctcacta actgaaattt gctcatcttc
      961 aatatctttt agttgagttt gtaaatcctc aagattttct tcaagaactg aataagcttg
     1021 ggttggttct tcttgatttg aagttacctc aacaatagct gcctcaaaac tctctaattc
     1081 tgtctgaata cgacgaacat ggcttgcagc tgtctcagga agtaaatagg tcttgttcaa
     1141 acgtgcaata tcttctccca ataaagtatt attctctttc atatgttgaa ggtaagttgg
     1201 aagagttgca agtagatttt ccactacttt ctgagcagca atttctcgag taaaaatatc
     1261 atacaaggca ttgatttcct cttgtgcctg tccattctca tattcggcat tatccaattc
     1321 caactgacga atattctctt ggtttttctt gaatgcttca taaagcaagt ggaaacgcgc
     1381 ttcaatatcc gtttcaacaa aatgataatt agcatcaatt agtttacgat aaccggcttc
     1441 caaatcctgt aattgatctg gcaattctgt agaaagcgtc gtaaccaagg ctggaacacg
     1501 atccacaata tgacttaagg ccaaaatgtg attttctgta ttatccaaaa tcactgcggc
     1561 ttccacaggg tcacccgatg aattcaaggt tacaaattgt gaaaattcag attggatatt
     1621 ttctaattgt ttttcaattt catccaaggc ttgaccatac tgttctgaat tttcagcaac
     1681 tctatgctga agttcctcaa ataaatccga agcatgaaga acacgaccac tatttttaga
     1741 ttcttgcttc tctaagtctg ccaaagcatt gcgaattgcc gcaatatctt cttcaatcaa
     1801 agtaatttga ctctcaattt ggtcaatttg atgactggcc ttgagaaaac gaaatgaatg
     1861 gttatagcct tctgcttcaa agagattatt ttcaatatcg gcaaaagagt tgagagataa
     1921 atcgacccat ttttgattcc attcacggaa agccacttga ctttgtccaa tcaagtgcat
     1981 attttttaca gcttctactt catcatttac tggaagattg tatagttctt cttttctttc
     2041 ttctagcgcc tctaatctcc cctcgtttcg cttacgtaga aagattgcca ctacataagc
     2101 cagaactaaa atgactgcaa ttgcaaccat taaataaatt agttgtccat tagacatatc
     2161 aaactccttt tttacttgaa acaatcgtaa caattatatc atatttttta gaccaaggga
     2221 actacttctc cctatttttt agacatcaag tgtactatag acagcatttt cttcgataaa
     2281 ctcacgacga ggctctactc gatcccccat caacatatca aagattttat ctgcttctgc
     2341 agcatcatct acagaaactc tagccatcaa gcgatgttcg ggatccatgg ttgtttccca
     2401 cagctgatga tcgtccattt cacctagccc cttataacgc tgaatagtcg gtttggtacg
     2461 accttcacta taacgggcta aagcttcttg gagtttgatt tcttgatctg cacccggttg
     2521 gatatattct ttaatctcgc ttccaacctt gacaccatag attggtggtt gggcaatata
     2581 aacataacca gcttctagga ttggtttcat ataacgataa atcaaggtta aaagaagggt
     2641 acgaatgtgg gctccatcga catcggcatc ggtcatcaaa acgagttttt ggtaacgggc
     2701 tttcgaaaca tcaaattctg cgccaaatcc tgttcccatg gctgtgaaaa gactacgaat
     2761 ttcttcgtta gctagaatct tatccatact tgctttttca acgttcaaaa tcttaccgcg
     2821 aattggaagg atagcctgaa actcacggtt acgaccagat ttggctgatc caccagctga
     2881 gtctccttcg acgatgaaga gttctgtttc agcagggtta ttagaagaac agtctgctag
     2941 tttccctgga aggttggaaa tttccaaacc agatttttta cgtgtgactt cacgcgcacg
     3001 cttggcagcc acacgagcct tggcagccaa aattcctttt tctacgatac gtttggcaat
     3061 ctgtggattt tccatgagga aatcggagaa ggcttcactg aagaggcgat tggtaatctt
     3121 gaccacttcg ctatttccca atttggtctt ggtttgtcct tcaaactgtg gatttgggtg
     3181 tttaactgag ataactgcag ctaagccttc gcgaacatct tcccctgtta ggttgtcttc
     3241 attgtctttc agtaacttat ttttacgagc ataatcgttg ataacacgtg tcaaggctgt
     3301 acggaaacct tgttcatgcg ttccaccttc atgtgtatga atattattgg cgaaactcat
     3361 gacattttca tggtaacccg ttgtatactg catggctacc tcaactgtga tatcatccat
     3421 ctcaccgtct gtatagattg gtgtatcaaa gattacatcc ttgttctcgt tgatatattc
     3481 aacgtaacta gcaatcccac cttcataatg ataatgcttg gtttgttcca aaccttggcg
     3541 cttatctgta attgaaattt gaagaccgcg atttagaaag gccaactctt gaatccgttt
     3601 atttaattta tcaaaatcaa agattgttgt ttcagtgaag atttttgggt ccggtgtgaa
     3661 gtgaacagtt gttcctgttt tatccgtatc tccaactatt tcaagttctg cgacaacatg
     3721 accacgacgg tattcttggt aatgaatctt accatttttg tgaacatgaa cgtctaattg
     3781 agtggaaagg gcattaacaa ctgacgaccc caccccgtga agaccacctg aaaccttgta
     3841 tccaccacca ccgaactttc ctccagcgtg aaggactgta aagacggtct caacagcagg
     3901 acggcctgtt ttttcctgaa tatcgactgg gataccacgc ccatcatcca caacagtaat
     3961 cgaatcatct ggctcaataa aaacttgaat atggctggca aatcctgcca aggcctcgtc
     4021 aattgagtta tcaacaattt cccagactag atggtgaaga ccttcttttg aggttgatcc
     4081 aatgtacatc cctggacgca tacgaacagc ctctaagccc tctaaaactt gaatttgact
     4141 ggcatcataa tcctgtgcct gcagattttt gatttcttct gtcatcttat tcctttttct
     4201 tatatgtcta atacttgtct taaattcacg atactggtaa acaagtgggt atcaagtcct
     4261 gcagcttgac ctgcttcgat atcaatcggc cgatcaccaa tgacaagacc agagctaatc
     4321 tgatactttt ctcttaaata aagcatggat tcgggatttg gctttctctt aaagcctgag
     4381 ctagaagtca ccacttctgt aaaataagct gctatagagg ttttttctaa aatttccaaa
     4441 acctgatcgt ttcgatgaga gaccaaaaaa tgacggccac cttgatttga aatgtcttcc
     4501 aataggtcag aaactccttc aaataaaatc gggtgttcaa gctctctggc ttcattttcc
     4561 ttgtactttt ctaaaaaatt ctctaaattg ggagcgaatg tctcaatcgc aaaaggagta
     4621 gaaaccttta aagcttgata gacactgtca tggtcttgtg tgataccata cagtgccaat
     4681 gtttcaacaa atgcagctgt tgaagtttca taattatcca gtaaagttcc acctaaatcc
     4741 cagatgtaat cgtgatattt catacccttc attatagcat ttttttatga aaaaagcgat
     4801 gatttccctg agttttccac ataaaaaacg agagaataac tcccgtttta ctgattttta
     4861 ccaaagacat ctcgatagag cattcctgtt cgtaaactct ctgcgtcccc tcctagttgc
     4921 tccaccaact cgtaggcaaa ggcaagggct gttgaaggac cccgactggt tgtcaactga
     4981 ccatctacca ctactgtttc cttgacgtag tgaccatcaa ggatttgctc ttgaacgccg
     5041 tcataacaag tgtatcgctt atttttcaat atctctgctt gattgagggc aattggtgcc
     5101 gcacaaatgg ctgctagttt cttcccttct tgctcgaagc tttgcaattc ttgaatcaag
     5161 gtctgattat cacgtaaatg tgcagaacca ggcatacctc caggaagaac aatcatatca
     5221 tagtctgata aatttccatc aaagacatga tctgctctta cttggattgc atgcgaaccc
     5281 gttacttgct cttcaaaacc aaccatatca catgtgatat tggctcgacg caagacatct
     5341 acaactgtca aggcttcaat ttcttcaaag ccctgagcta acataactgc tacttttacc
     5401 atgattttct ccttatttaa tttgttttaa tacaaccttt ttccgtttga atgggacttt
     5461 tccgctcttt tcttcagcta gattagtctg gtaactcatc gataaaagca aaccaacacc
     5521 aatcagatta ctgataatag ctgatccccc ttgcgaaatg aaaggcaagg gaatccccgt
     5581 caaaggaagt agtccagtca cagcaccgat attctcaaag atgtggaaga gcaacatcat
     5641 aatcaaacct gtggaaatat aagtgtagaa ctggttattt gatttaagag taatcttcaa
     5701 catacggtaa atcaacatga gatagagggc aataaccagg acagagccaa taaagccaaa
     5761 atcttctgca ataaccgtaa aaatcatatc tgactctcga actgggataa gcagattcga
     5821 agcattaaat ccctgaccaa ataagccacc actcccaatg gcaatctgcc cttgagcctg
     5881 ctggtaagtc gttgtttggg caaactcaaa gggattgagc caagccaaaa tccgattgat
     5941 ttggtaggtc ggcattccaa tctggtgaag aaaagctcgt ccgtccttgc taataaagat
     6001 agctaagaaa ccagcaactc ctgttacagc agtcacaaat actgggataa taattttcca
     6061 agaaacccct gataataaaa cgattcctga gaaaatggct acaaaaacca aagccgtccc
     6121 caagtcactt tgaagtgcta aaagaactag gactggaatg gtaaagagaa tcatccagaa
     6181 aattaacaaa aagtccagcg gaaccgtgcg tctccattcc ttatgtttct ttgtaaattg
     6241 gacaatgaca cgagccaaca tgaggatata ggatatcttc ataaattctg acggttgaaa
     6301 tagggtaatt ccatttattg atacccagtt tttggcaccc gttgatgcaa ctaagcttgg
     6361 attataaaat acaatcggca agatcataag tcccaagcct aaaatatata gaaagggggt
     6421 caccttccaa agaaattctg tattaaagag catgaccaca aaaccaatca caagccccaa
     6481 ggcaatccag gcgacctgct gccctaaaat gggcagaata ttattggggt aatcatgact
     6541 aacggctata tagatagcca ccacaccgat gaccagtaga aaaaatactg gcaagagcaa
     6601 actgtaatcg actcttgagt cgagagaacg tttcatatat actaacctta tactttcata
     6661 caatactatt tatcaaagtt cattaaaaaa tctatcaata gcctcgtcaa cttcggatcg
     6721 agagatggtt ttaacagtcg cttcttctgc tagagatgct actatttgtt tgccgtatcg
     6781 ttttccgacg attctcctat ccaaaataag agttaaggaa cgttggtatt cacgtctcat
     6841 acttcttccc aaagcctgtt ttaaacgaat aatggccatt ggcaattgat aatcataaaa
     6901 ggcatttttc ccttcttgat tcagttcttg attaatcttt ttcgtcaagg gttcttgagg
     6961 attttggaaa ggaagcctcg gtacaacttg aatcacaaaa ggatggcttg aaaaatcaac
     7021 tccctcccag aaacttgctg caccaagcaa gatttgttgt tcaccttttt caaagcgttt
     7081 ctttagctga tgaacatccc catttttata ctgggccaag tggctaactg taagtaaatc
     7141 cgatactgct agaagcatgt ctttagcggt aaagagaacc aaaatcggtt gctggaaagc
     7201 ttgaatttcc actagtaaag cagctacctc tctggcatag acttctaagg aggtttctgt
     7261 taccagaggg aaatctttga ccatgaccac ttcttgttcc tgttttcccc gagattcaat
     7321 cttgacaaat ttagcttcag gatagcctaa aaggtctgcc aaagaaaccc tctgactaat
     7381 ctcaagagta gccgatactc ccaggacttg gcaactctca ggaagtaaag aggacagaag
     7441 agtacggcct gattttgtag aagaaatctg aattttcttt tgactcgttt cagttacttc
     7501 aagccagtaa tcaccttcag ctgtaaaata gcgaaccagc tcatcaaagc cttctacttc
     7561 taattctgaa aaatactgat ggagattatc cagagaatct aagatatttt tcctagattt
     7621 gccagactga aattgttcta tcaagtagag acactcaaag cgaatacttt ctagtatccg
     7681 ttgttgaacc ctgttttctt ctcctactaa agccttatca attaaatcga taatagattg
     7741 tatatcgtag gtctcttgaa gcagattttc tagagctaac aaaatctttt ggacttcatc
     7801 aataatcagt aaacggtcac tgacaaattc aggattatct tcaagtctgg tcacgagata
     7861 ggcatgatta gtcactaaaa gcttgcaagt ctctgccctt tcttgactac gtttccaaaa
     7921 atcttccgtc acaaataagc tatgggatga taaattccca tcatgacgaa ggtctgctag
     7981 aaaatgttgg taacggtaga gttgcccgat ttcatccaaa tctcctgtct ctgtctcagt
     8041 aagccagacc aagacttgca ttttaaagcg tctaaataag cgattttcat cattttcctg
     8101 caaggaatga taaaaggcat ccaacttcag ataattttgt ggtcccttta agctatgaat
     8161 atctgtatgg aacacttcct tgagacgttt accttcttct tccatgattt gattttgaag
     8221 aatctttgtc ggaacactaa gaacaatttg tcgctctttg gattgagata aagcgggtaa
     8281 gagatagcca taggttttcc caatccctgt cggcgcttga atcagagaga caggttcatc
     8341 tttcaatagc aagccaacct ctttagcaaa actttcttgt tcctccctca cttcaaggtt
     8401 caacagagaa atatttttag aaaagtcttg agatagtttt cgtagctcca gagaagctgc
     8461 cgttttctta aaatacagac cttgaacttg gaccaagtct ggagaactca ggatagattg
     8521 gttgcgataa gtttcctcaa taaccaggta ggactcatat aggagagcgt cagccatttc
     8581 cagcaagcgt tccaagagac ctttaggaag ctgggtcatc ttttcccgta aaaaaagaag
     8641 taattctgct gtagcttggg catctgaaag ggctgtgtgt gcgtgtttaa gaggaattcc
     8701 taattctcga cacaaaatcg gcaagctata tttttccagt tcagggaaaa agacctgggc
     8761 caattcgacc gtatcaacac gagggtttct tagctcatag ccttcaaaaa ataaattttc
     8821 cgccaagaga ttagcatcaa actgaacatt atgggctaca aaaatcccat cctccaccaa
     8881 gtcaaagatt tttctggcaa cttgcgaaaa atcaggtgct tgcgccagac gttggtctgt
     8941 caatcctgtc agttctttga tatgagcatc caagggttca tgtggattga catccgtcgt
     9001 atagtgatcg acgatttctc catcctcaat cacgacaatt cccacttgga taattttagc
     9061 cttactacct gtgctagttg cctctaaatc caccacaaca tagcgattac caatcgtttt
     9121 cattataaaa aattatacca aaaaaagggc catttggcac ctgcaaacat aggataattt
     9181 tcaaactcaa gaacactact tttcttagaa atcccatcga atagagcatt ctagcctgct
     9241 gattttttta aaatagtgaa gaaatacgaa aaattgagag aagaaatgtt ttcatcttcc
     9301 ttctctcaac gaatcatgta ttttatttaa ccatgtcagg atcgtagtca taaaatcctg
     9361 tatcaaggaa acggttttta gggtgaagtt tacgaacttc ttcatccagc aagaaggctt
     9421 ggtcatggct aaagttgccc tcttgaatca agctacgcgc ttggataaag agttttgcaa
     9481 tctcaacaaa gttctgacca ggagtgtaga gcccttctag tttccagcga gtaaagccat
     9541 gctccaccaa ttctgttaat ttgatcatca aatcaaggtc attgttggca aagatatggg
     9601 tcccatgatt atcttcaaaa atggaatagt ggctctctgg atcacttggc tcagccaaga
     9661 agaggtcacg tttatgcgtc ttttcatcat cgatatgtgt aaagttatag tagttttgca
     9721 agagtggacg tttagaatga tggatgacgc tagcaccgta aaccaaaact tcagcaggaa
     9781 tttccaaaat ctctggcatt ttgaaaagtt cagctgatgg aatttcacgc gccaaaacag
     9841 cctcagatgc gccagccttt tgtccccaga agttaatctg acgactgcta gttaccatgg
     9901 ttgaagcatc gtagatggtc ttaaatgaat aaccatcgcg gttaactacg taaaagacgc
     9961 ctgcatcccc aatcgtaata tagtctgtct tgatttcttc caagaagtct aagaaaggct
    10021 tgatacggtc catcatatct tggtgcatga gagcattgac cgcaacgagc aattccttac
    10081 cagcatcatg aaccaactta gcgatttcac gtaattggtc ataactaaag gtcgttggca
    10141 gacgaaggcc aaaatctttc tcaccgacat agatacggtc tacgccagct tcgagtagtt
    10201 gttcaacttg ttcaatactt tcagcagttg ctgtaatgac aatcttttcc ataagaaaaa
    10261 ttataccaca tttctccaaa atcatccatg ctttgaaaat gaaaagggac tttattcttt
    10321 ttgtaacctt tctgtaataa ttttctgccg aaaaaatgat aaaataggta tgtactgtta
    10381 aggagagaat catgcccgta agaaaattac aatcctatga ggtagactat caagaagaat
    10441 taaaccagca gcttcctcat taccaagctt atacacctga agcgcaatct gatgccaatc
    10501 tcaaggaaat cctatttttt attaatatcg ctgttttttg tatctgtatt gctatcttta
    10561 gttttatctt tttagcatta aaattatcaa ctgctcttgc ctttgccgca gcaatcggat
    10621 tcagcttact tgttttaaaa gttcaacggt ctattatcaa acgaaaacgt agaagataaa
    10681 tccaaaatat cgccccaatt ggagcgatat ttttattttt tctttttaga tggtgtctga
    10741 atggcaatct tcacttcaaa gattgttccc ttgggtttat tatctttgac agtaacagtt
    10801 ccttttagag catctacaat ttgcttggct agggataatc ctaaaccaaa accacctttt
    10861 tgccgggttc tagccttgtc tactcgataa aaacggtcaa aaattttctt tttatcttct
    10921 gtcgaaatac cgattccatt atcagaaaca agtaaataaa gattgcgatc ggtcgccgag
    10981 ataagaaaat caatttcacc atcctcctca gtatacttga cggcattatc gaaaagaatg
    11041 gtcatcagtt gtttcagaag aagctgatct gtgacaattg ttcgatggat acgattttca
    11101 aaacggaaga cacgattatt ttccgaagca atcatctcat agtttgtgaa agttgtatta
    11161 aaaaagctag ttggaacttc tgcaagctcc ggcttaatcc catcatctct ccgagctaag
    11221 ttcagcaagc ttgtcgttaa aaaacgcata tttcggactt cttccaaact cgatgcaatg
    11281 ctttcgctca catccataat ggtagcttct ggcttacgaa aaagggtctc taagcgattt
    11341 tgcaaaactg cgagtggagt tcgtaactca tgactggcat tttccacaaa agactgttgc
    11401 ttctgcatac tctcaagcag gggcctaaca ctgaccctag ctagatagag actggcaagt
    11461 aaagacaaaa tccagaaact agccatcacg accacaatca attgctcatg cttttgacta
    11521 gcctgctcca actgactggt attaatcaag acagcagcat acttgatatt ggttgaaacc
    11581 gaactaatat tggtctccat caaaatcaca cgatagattt cttcctgccc atagctatta
    11641 aaaacctgaa tctggtagat atgtcctagt tctttctttt ctaacttaat cttatccaag
    11701 cccaaaaatc gatttccaga aagaagttga gtaaagtctt tatcaaagag aatgacttcc
    11761 gtattggaac tgacattagg ttttatttct actttactag cgtccgccct agcattttct
    11821 aaatctttaa tctcttctgt tgccctattt atagccagct gaataactgc ttgaggattt
    11881 tcactcagtc catgaagctt atcgtccacc gaagtataaa gactcgaatg catgacttgt
    11941 aaaataatca gagtcattgt agaaaaaatc agggtgaaga caccgaagtt gcggataaaa
    12001 taactaaagt catccgcata ccatgttttt ttaagtttac tgaacatctt ttaaaagata
    12061 cccaacacta cgcaaggttt gcaaattctc tgcaaaagtg gttcccttta atttcttacg
    12121 gacttttgaa acatagactt cgacaaccga aatcgttgta tcactatcaa atccccatag
    12181 acggtcaaaa atctgcgtct taggcaaaat cacattttga ttttgaagga aataaactag
    12241 taaatcgaac tctttcccca gcaattcgac aggagtatct tcaactttaa cggtattggt
    12301 tgataaatta accacgatat tcccataagt caaggtgttt tcattaaact tccctgaacg
    12361 tttgagaagg gcctgaattc gcattttaag ttcttctagg tagaaaggtt tggtcagata
    12421 atcatccgct cccagttcaa atccatgtcc cttgtcatcc aaactttcct tggcagtcat
    12481 aatcagaact ggtgtcgtaa ttcccttttc acgcaattct tttaagactt ggaaaccatt
    12541 tttttctggc aacatcaaat ccagcaaaat caagtcatag acaccactct cagcttcgta
    12601 gagaccttct tctccatcaa atacctgcat aacatccgca aaatcgtcta aaaagtcaaa
    12661 tactgaattt gacagaccta ggtcatcctc aaccaataag atttttatca tgagaaactc
    12721 ctccttatta aaactattat accaaatttg ccttaaaaaa aactcaactc tctgcatttt
    12781 acatgagata gctgagtttt ctttttattt taggcttatt tatgcatttc cgtattgaag
    12841 aacaactgct tcgactgcag ctttttcacg gctaatcaag tcaacacgtg ctgcaatttc
    12901 cttgattccc ataccgatgt tacggctaag agcaaggtca gaaagttgcg gctcaaagaa
    12961 ctccttgtat tccgccaagc gttgctgagt cttaaataca tgagcaggaa ggataacaaa
    13021 gctatcaaag ctcatatctc ctccaagggc tgccttaatc caagcccagt tttcacgcgc
    13081 ccaagaccaa gctgttttct gagttgcttg atgagctagg aattggtaat accaagcaga
    13141 caagtcctgt ggtttgacca caaatttgtc cttccaagaa gtaatcaagt tttggatatt
    13201 atccgcatct gtactgtatg caagagctgc tgccaactgg cgtttaaaga cagcatctgt
    13261 tgcgtgagta taagtatcaa gataaagtgc taacaagtct ttagtctcat gatgtttcat
    13321 ctcattaatc agaacttgtg aacgaatagc tgctgggagt tctgcaagat tctccttgtg
    13381 tgttgcgaag atttggctag cgacttgacg agcttctgca tcatttgagc gaatcatcat
    13441 cgaaacagcc agctgacgaa ccaattcatc ctcatctgat tctccgtctt tagcttcaaa
    13501 accaagacgg tcatagttat gacgagccaa tttagcaacc agtcctttga aggctgtttc
    13561 agcatccgtt ccttcatcaa taaagcgctc aagggctgaa atcacttgag aaacagctga
    13621 aaccaccaga taagactctt ccttagcaag tttatcaaga actggaagca agtctgcata
    13681 agaaatgtgc cctgcctcag ccaacaaacg acgttcttga acaatttgca gtttgcttgt
    13741 gttatcaagt gtctctagct cagcaagaac agcttctaac aagtctcctt gatagtcggt
    13801 aatatagtgg gcagtatttt cagtgttgag acgaagagct ccttcatttt cagcaagaag
    13861 agctgcgtag ccagggattt cgatactttc agtttcgagt gtatcaggca agcctttcca
    13921 gttgctattg aggggcacca cccagagacg gttcttgtct tcgttctcac cgatgaagaa
    13981 ttgtttttgt gaaatcttca agacatcatt ttcaactttg acagtaagaa ctgggtaacc
    14041 aggctgttcc aaccaagaat ccatgaaggc tgcgacatca cgtcctgacg cttgaccaag
    14101 ggcatcccaa aggtcactac caatggtgtt gctgtattgg tgtttttcaa agtaggcatg
    14161 caaaccttta gcaaaatcag catctcctag ccaacggcga agcatgtgca tgagacggct
    14221 tcctttggca tagacgatag cgccgtcaaa gagtgtattg atttcatctg gatgtttaac
    14281 ttcgacgtgg acagactgaa cgccatcagt agcgtcacgt tcaagagcaa gaggtactcc
    14341 acctgtttgg aaatcttcaa agatattcca gcttggttcg atggtatcca cacagacgta
    14401 ttccatcata ttagcgaaac tttcattgag ccaaaggtca tcccaccatt tcatagtcac
    14461 gaggttccca aaccattggt gagccaattc atgggccaca acaagggcaa cttgttgacg
    14521 gctagcaaat gtagagttct catcgacaac caagtaaact tcacggtagg tcacaagacc
    14581 ccagttttcc atagcaccag ctgagaagtc aggaagggcg atgtggagag attgaggaat
    14641 tggatactta actccatagt aatcttcgta aaactcgata gagcgaacag cgatatccag
    14701 tgagaaatca agatttgaaa gtggatgtgc tttggttgag tagacaccta ccagggtacc
    14761 atttttagtt ttagcggtca ccccttgcaa atcaccagca acaaaggcca acaagtaaga
    14821 agacatgcga ggtgttgtct caaacttcca gatacctgtt tccttacggt tttcaacatc
    14881 gatttctggc atgtttgaca aggccaattc accttctgct tggtcaaagc gaagagagag
    14941 gtcaaaagtt gctttggctt caggctcatc cacacatggg aaagcttcgc gcgcaaaatg
    15001 gctctcgaac tgagtagaca agacctcctt cttgactcca tcaactgtat aataagaagg
    15061 gtaaatccct gtcatgttgt ctgtaatttt accagaaaag gcaagaacca attcaacttg
    15121 accagcctca gccaattcga tatgaagggc ttcattgtca tggtcaactg taaatggacg
    15181 agcttgacct gcaacttcta cagaggtgat ttccaagtct ttttggtgga gggagatgcg
    15241 gtcactctgt gcttgaccag tgatggtcac tttcccagaa aaagtcttgg tctcacgact
    15301 caaatctaaa aataaatcat aatgttcagg aacaaattgc ttaataaaat gttcaactgc
    15361 ttgcatagtt ttctcctatt ctaagtttaa gagctgctgc tcttcttcaa acaaacttat
    15421 tatatcatat tttgcttgaa aaaaaaggat tggacagcga tttccaaaac tttcttcctt
    15481 ctaatagtct acagtgggta gaccttgcga aattcttcta aaacaacctt gctttcagat
    15541 gtaaaagtca gccctacttc acccaaacag tctgtgaaaa agtcctcatg aacctggcgc
    15601 caataggcaa gtgatttgtc tccctcacct tccttaaagg catgttgcgc agaaacttga
    15661 ttgaagagtt caacagaaac ctttgtaatt tcgacaatgc agacagactg attttgacta
    15721 tctaaaatga tatcgaaggt ccctacttgg ggaagtggtt cgtcttctag tacatagagg
    15781 tcataggctg atgctgttgc tgtcttttct cctttaaaca ccaaatccgc taaaagatct
    15841 ggttcaactc caaaagccca agcatcgatt tcatctccga tcaaaggatt gatttgcttg
    15901 tatttattcc acatttcttg cggtatcatg ggtactcctt tgtaattttt tactttcttc
    15961 ttttatgtgt ttaagatgat ctggatggtc aatctctaaa tcaaaaatct ctggaataga
    16021 actgtagtgg ataatgcact tgatacccaa ctgattcatt ttttgtatga aagaagtatt
    16081 cagatagcct gctacagcaa aatcaatctt gttctttctt gctttatcct gcatatctct
    16141 tagcatatct aacattattg gactttccat atcatgccat tgactgtttc tcatagtcgc
    16201 aaaaacaaag gaagtcaaat cattcattcc aactacaatc tttgaaatgc ccgtttccag
    16261 tatactagat aagtcaaaat acgctgacgg taattcaatc atcgttccga ctttcccagt
    16321 aaaaccctgc tgacgcaata ctgtaatagc ttgttttaat tggtcgtcat cattgacaaa
    16381 aggaaagata acagatagat tggggttggt ttgataaact tctgtaacga catgtgcttc
    16441 agcctgaaat tcatccaaac acgccagtaa acgcctggtt cctctatagc caaacaaggg
    16501 atgcccttcg tcaaaaaact ctctagtccc cactaaacaa ttggcttctg tattcgttaa
    16561 ttcagaaaaa cgataccaaa cttcttcatc tgagtacagg gagcagatag tctctagata
    16621 atcttttaca aattgctgac aacttgctaa tatgatgttt tgattaagct ctctcaacaa
    16681 atattcccca cgaatcatgc cgacgtggtg aaatagttga ggataaattt tttcaagaat
    16741 tttttcgcca ctaagggcaa gttgatttct catagccatt cacctcgtct taaaagatac
    16801 aatatcatat ataattatat caaaatttcc tctagttcaa aagcttcata aggttcagat
    16861 aactcatatc ctaaaaattc tgctactttt ttggaagcct cattatgagc atcccaaagt
    16921 ggaaacatat ctctatttaa actctctaga atcattgcag caccaagctt tttagcaaat
    16981 ccatttcctt gttcgtttgg tctagttgca acttccactt caactgcttt acggtaaact
    17041 aaccctgagg aaatcccagc aatcagttca ttatttttaa gaatcacaaa gccaaatcca
    17101 ccttttaaaa caaaatcctg ataggactca aaatccccct gtaaatcctg tgaccattct
    17161 tccgtagaaa agcagttata aatatgatta tcaataggca caatattgta accttcctct
    17221 aaatgagtaa ctagattatt taaaaattca acttgaaaat ttgccttatc tttaaaagaa
    17281 tagcgagtaa aagaatttag ccccactttt cgatgcagaa aatcttgcca attagtattt
    17341 tctgaaataa taatcttata atctaaacca tattttctaa caaaatcttc ccaaaatctg
    17401 gaatccaact cccctgctag gtatagaaaa ttcccaacat cataaaagct tgttgtccta
    17461 tcattgtttc ggtagacagt tccaatttga gatttaagac catagagaac catatttttc
    17521 ttccatgttt taaatagttc cattatccct ctacttcacc ttccaattca tgtaagaagt
    17581 cttgtccagt tctggcaatc ctaataattc agacttaacc ttcaagacta atggcgatgc
    17641 attttcttct gtaatctctt gaatatccat ccaaatatat ccaagtgaat cattcgcacc
    17701 atcagacaca gcttccgaaa tcgtaacttg aggtgcactc tcattcattt caacatcata
    17761 caaggctatg acatggtgaa ccataaaatt ttttaactct tccctgacga aaacatcgta
    17821 gattcgagga ttagagtagc ttctaacagt aaatcccgtc tcttccataa cttctctagt
    17881 cagcgtttcc gtcagtcctt caccaagttg ctgactgcct ccaggtagat cataccgatg
    17941 ttgataaggg cctctcgttt tttcaatgca aagtaacttt ccattttcaa agcaaacaca
    18001 gtagacccca aagtgatttt tgatttccat ccaactcctc ctacttcaaa gaccagccac
    18061 catctattgt cagaatttgt ccctgcatgg cagatgcctt tccactggcc aaaaagagac
    18121 tgacttctgc cacttcctct ggttcaatcc agcgcttgat tggtgtttca ctagccaccc
    18181 agtcagctaa gccacctgtc tcaaaatccg cagcggtcat agctgtcttg actgctcctg
    18241 gagcgatacc aaagacctga atcccagctt cagcatagtc tagagccaac tgcttggtga
    18301 agccagccaa ggcatgcttg gatgaagtat aggcgtgacc acctccacct gctaggctag
    18361 aagcaatgga acacatattg atgatgattc cctttttatt ttccagcatt tgtgtcaaat
    18421 aataccgagt caactctact ggaataatgt agttgatttc aaaaatctct tgaatgtcct
    18481 gcgccgtttg ttccaacagt ggtttgtaat catccaaaac tccggcagta ttacacaaaa
    18541 catccacctg agggcaccag tcaaaaatag gttccaagtc caaggtcaaa tctctctgta
    18601 aaaagtgaaa atcaccctct aagagtggct tttcaccttg gtcaactcca taaacttgat
    18661 agcccttctc taaaaagagg cgagcttgag ccaatccgat ccctgaactc actcctgtaa
    18721 tgagtacacg tttagtcatg cacttctacc caatccgttg ccaaaacatc acaaactgtc
    18781 gggctccaca tggaaaaacc ttctccttcg ccagaaacgt tgattaggaa ataaggtgtc
    18841 atttcaagtg caagcccatt ttgctcgatg gtatcaaaga gttggacata gttttccgca
    18901 cctccccaac cagttcgtac atattttctc ttagccttta acccaggcag gatctcttca
    18961 aatgtcatgt ttttctcctt taattctaca ttcttcattt aattatagca aaaaaccact
    19021 ttatacggct ttttgactgt gagttattca aacctgctac tacttacggc aaattattcc
    19081 ctgcagcaag ataaatttca taccattctt ttcttgttaa gctaaagttt gccgctcggc
    19141 taacttctct caagtgctta ggatttgttg tacctacgac tgcctgcatt tttgctggat
    19201 aacgcaatat ccaagaaatg gcaataattg aagaggttac tccatattta atagctaaac
    19261 gatcaagtac ttgatttaaa gcttgaaatt tctcatttcc aacaaagttc cctttaaaat
    19321 acccgaattg caagacagac catgcttgaa tgaccacatc gtgtaatttg caatattcaa
    19381 aaatgctgcc atctcgcata gctgcttgac tatcttccat attaacatga aaagctgatt
    19441 caaatcctgg agtaaaagcc gcactcaatt gtagctgatt aacagctaac ggctgcttga
    19501 catctttttt aagcaactcc atcatcatag gattttgatt agaaactcca aaatctcgaa
    19561 ctttaccttg tttataaagg agatcaaagg cttctgctac ttggtcagat tccatcaaag
    19621 catctggtcg atgaaggagc aagctatcta gatgatcaat cttcaatctt tgcaaaatac
    19681 cgtctactga ttttataata tagtccttag aaaaatcaaa ataggtaaat tcttcaatgc
    19741 gaatgccaca tttggactga atccacatct tttctcttaa atctggacga ttttttagga
    19801 caagacctaa cagttcttca caacgaccac gaccataaat atcagccaag tcgaaggcat
    19861 tgattccaac agaaagtgct gtttctacaa gctcttcaac ttcttttaca gacttatctt
    19921 ttattctcat cattccgaga acaatttctg ataattcttt gtcatcttga ccaagagtta
    19981 tgtatctcat caaatttttc tcctttaatt tctaacattc ttcccttcat tataacaaaa
    20041 aaccgctttg caacggcttt ttgactatac ttcactccat tttatcttct taaacccacg
    20101 gaacaagaca aagattccaa taaagaggac agctaaagga ataacttttg taaggaaaac
    20161 atttgaaatt cccatccact cataataacg gagcagagaa cccaccacaa gatgggcaat
    20221 aatcatactg acaaatggac gaaagaccgc ttctttccaa ttccaaatac cgataactag
    20281 cgaaatcgta aagacagaca aactatccca gggagccgga atataaaagg ctccttcttg
    20341 tatgaagctt gccattccta catatcctaa aacaactaga agaactatag tcccaacaac
    20401 aatgtaagtg ccaattttca ttttaggaga atcttggact aaacttcttc gtaaaattgt
    20461 ggccacaagt ccaaatccaa tcagaaaaat aagaagttgc cctaaaaatg tgagcaaatt
    20521 gactgttaag agaggacctt tagaaaaatc acttagtagt tgataataac gtaataccgc
    20581 caggacaaga attggcgtca aaagggactc tttgatagaa ctgcgaggtg ctcccttgag
    20641 aatctctttc attatttttt taggattctt acctagataa tcctctgcac tcatgccatc
    20701 tcgttctgct tctgagaaat ctagcatcat caaatagatc tgctctctga gatagtcttc
    20761 atcatagaga aatccagcaa gattaaaact ttcccacaac tcctcaaaat acttttgatt
    20821 ctcctcagaa aactcatgta gcaaagcgct tgtttcttcg taatacttca ttttcttcat
    20881 ggtttaaccc ccattcttaa tcccttctac tttttgactc aaatcgtccc attgttgcca
    20941 aaagactgag acacgctctt ctccttcttt cattaatgaa aaatacttcc gatctggacc
    21001 atctggcgac gggcgcatgt cgcctcttat ccattgattt ttttctaact tttgcaacaa
    21061 aggataaata gttcctggaa cgatagtatc aaatccagcc tctcgcaaag tctgaaccaa
    21121 ctcataacca taccgctctt tttgaccaat catatccaag acacaacctt caagaacacc
    21181 ttttaatagc tgagtttctt tcatcacttc tcccttctaa tctattttgt aatacctact
    21241 agtaacttca cctatagtat atcacttcta cactagtttg taaagcataa tagttaatac
    21301 tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccctac cgtatgtatg gttactgact
    21361 tcgtcagttt catctacaac ctcaaaacag tgttttgagc tgacttcgtc agtttcatct
    21421 acaacctcaa aacagtgttt tgagctaact tcgtcagttt catctacaac ctcaaagcag
    21481 tgctttgatc aacccgcggc tagcttccta gtttgctctt tgattttcat tgagtataaa
    21541 taaaaaaaca gaactagcct gaactagtcc tgtctacttt tacccaatca cacttccatt
    21601 tggtacagct ggatcaactg tgagaagggt taatttgcca tcatgttcag ctgagagaat
    21661 cataccctgg ctgacatatt ttttcatcat tttacgtggt ttgaggttag caacgatttg
    21721 aactttcttg ccgaccaatt cttgttcatt tggatagtat tttgcaattc ctgaaagaat
    21781 ctgacgatct tctccatcac cagcatccaa gcggaattga agcaacttat ctgaaccttc
    21841 tactttagac acttctttga cttctgcgac acggatttca accttgtcaa agtcttcaaa
    21901 cttgatttca tccttgttta gtttgagctc aacttcgtcc ggattccatt ctttttcgac
    21961 tgctggttta ttgccttcca tttgttcctt gatataggcg atttcttctt ccatatttag
    22021 acgtggaaag ataggtgttc ctttggcaac tacagtcaca tctgctggga agtcagccaa
    22081 actcaagttt tcaagactag aaacttcttc caaaccaagt tgagtcaaaa ctgcacgact
    22141 agtttccatc ataaatggtt caatcaagtg agcaactaca cgaatgctgg ctgccaagtg
    22201 gctcatgaca cttgccaatt ggtcacgaag agcttcatcc ttgtccaaga cccatggtgc
    22261 agtctcatcg atgtatttat tggtacgaga gatcagagtc cagactgctt caagcgcacg
    22321 tggatagtca actgcttcca tgtgtgtatg gaagtctgcg attgattttt ctgcaacctc
    22381 agcaagaaca tgatcaaatt cagtcacacc ttctacatag gcagggattt gtccatcaaa
    22441 gtacttatta atcatggaaa ccgtacggtt aaggaggttc ccaaggtcat tagccaattc
    22501 atagttgata cgaccgacat agtcttcagg agtaaaggtt ccgtctgaac caactggaag
    22561 gttacgcatg aggtagtaac gaagtggatc tagtccataa cgctctacca acatttcagg
    22621 gtaaacgaca ttcccttttg acttagacat ttttccgtct ttcatgacaa accaaccatg
    22681 ggcaatcaaa cgatcaggta atttaacatc caacatcata agaaggattg gccagtagat
    22741 agagtggaag cgaaggatgt cttttcctac catatggaag actgttccat tccagaactt
    22801 gtcaaagtta ccatgttcgt cttgagcgta gccaagagct gtcgcatagt taagaagggc
    22861 atcaatccaa acgtagacaa cgtgttttgg atttgatggg acaggcactc cccatgtaaa
    22921 ggttgtacga gataccgcca aatcttccaa acctggctcg atgaagttgc gtagcatttc
    22981 attaagacga ccatctggcg tgataaattc aggatgagct ttgaaaaatt cgaccaaacg
    23041 gtcttggtat ttgctaaggc gaaggaagta tgattcttca gaaacccatt caacctcatg
    23101 acctgatgga gcaataccac cagtcacatt tccagcttca tcacggaaaa cttctgccag
    23161 ctggctttct gtaaagaatt cttcgtctga tactgaatac caaccagagt attcacccaa
    23221 gtagatatca tcttgagcaa gtaagcgttc aaagacctgt gcgacaactt tttcatggta
    23281 gtcatcggtt gtacggataa atttatcgta tgagatatct agtaattgcc agagttcttt
    23341 aactccaacc gccattccat caacataggc ttgaggtgta ataccagctt cttccgcttt
    23401 ctgctggatt ttctgaccat gttcatcaag acctgtcaga taaaatacat cgtagcccat
    23461 caggcgtttg taacgtgcta ggacatcaca tgcgatagtt gtgtaggcag aaccgatatg
    23521 aagtttccca gatggatagt aaatcggcgt tgtaatataa aaattttttt cagacataat
    23581 ttttcctttc caggcaaatg aaacctgttt ttctaacact tcattatatc acatttttaa
    23641 tgaatttcga tagggaaatc catacaaaaa caagatagac gagtgtccat cttgttgatc
    23701 tcattcataa cgaagggctt caattggatc aagtttcgat gccttgttgg ctggcaagac
    23761 tccaaaaatc ataccaacac tagccgaaac tgcaagacta aatagggcaa ctgggattga
    23821 tactccaact tctatacctt ctattaaacc ttgcagtaac aaacctgcta aggcagttaa
    23881 accacttgca attgtcaagc caattaagcc acctaacaag gtcaaaatca tggattcaat
    23941 caaaaactga attaaaatat tggcacgtgt tgcacccaaa gccttacgaa gaccaatctc
    24001 acgagtgcgc tctgtcaccg aaaccagcat gatgttcatg acaccagttc ctccaacaaa
    24061 gagagaaatc cctgcgatgg aactaataat cgtcgtcata aaactaaacg attgttgaat
    24121 ttctgcaaat acaacggact catctgccac ctggtattct ccctgttgta agcctgcaag
    24181 ctctgtcatt tttcgtgcca gttctggacc cagagttggg gttaaactgg tatcattcac
    24241 tcgaaagaca atactagcta tttcatctat attaaaattc gcagcaaggg agatattggt
    24301 agtaataggc aagccaccaa acccatatat ttttgacctt ttagcctccg gactagtata
    24361 aaccccaatg acccggtaac taaatccatt gacttctaca accttgttaa tagcctcttg
    24421 aggagattca aataaactaa tggacaattc ctcatctagc aaaataacac ttgcaaactc
    24481 tttgaaatct tgctctctca gactacgacc tgcaataatt tcattcttaa cagcgtccat
    24541 gtaagttctg tttccacctg tcaaattagt attctcaacc tttttatctt gataggtcaa
    24601 gatggcattc gttgaattgg ttacatagta actatccact cccttcagtt tagctgcctc
    24661 ttgaacccag gattcttgcg gttttggcgg ttcaacagga acttcctctt cctttccaga
    24721 aaccgtaaaa gctgattgtt tctgagtaaa agacccatct ttactttttt taggagagaa
    24781 aaagacgcta atatttttct gagatttagt catatcttta ttgacttgac gagataggga
    24841 atcacccaaa gccataatca caacaactga tgaaacaccg ataataatcc caatcatagt
    24901 aagcaaagaa cgcatcttgt gagccatgat agatgaaaag gcaaatttca gattctgcat
    24961 cttagttttc ctcctttcct aactgagcac tgtcagacga aatgacccca tcccgaatga
    25021 caatctgacg tttggcatag gcagcaatct caggctcatg cgttaccatg ataatggttt
    25081 ttccttcttt attcaaatca accaataatt gcataatttg gttacctgtt ttggtatcca
    25141 aggctcctgt cggttcatcc gctaggataa tagaaggatt gtttaccaag gcacgcgcaa
    25201 tggctacacg ttgcttttga ccaccagata attctgaagg taaatggtga ctacgttcta
    25261 tcaattcaac cttgtctaaa tattcctcag ccaacttgcg acgttttgaa gacgaaactc
    25321 ctgcgtaaat caagggcaat tctacatttt gcagagcatt gagcttcgat agaagaaaga
    25381 actgctgaaa gacaaaaccg atttgttggt tacggacctt agctagttgt ttttcaccaa
    25441 gcccagccac ttcttgacct tcaagataat attctccact ggttggtgta tccaacatgc
    25501 caatcgtatt catcagagtg gacttaccag acccagatgg tcccatgatg gctacaaatt
    25561 caccctcatt cacttctaga ttgatatttt tgagaacctg cagttcttgg tcaccattac
    25621 ggtaacttct gaagatattt tttagactaa ttagttgctt catcagcctt cacctctttt
    25681 ccttcttcca aggaagatgt tggattactg atgaccttag caccgttcgt taaaaccaga
    25741 agtgatttct tgattttctg cgtcagcatt tcccaatgaa acctcaactt ttttagcctt
    25801 ttgttgttca tccacaatcc agacataatt tttactatca tccattacta gactgctaac
    25861 aggaacaaga atagccttag ttttgctttt aacctcaatg ttgacagaaa aaccttgttt
    25921 caaatcacca acctcgcctg tcacatcaat agtataaggg tatttagaac ctgtattatt
    25981 cccggctgct ggactagctg cttcaccatt gtttttagga tagtcagaaa tatagcttaa
    26041 tttcccagtc cattttttat caggatacac tttagaagta aagcttactt cttgacctac
    26101 agaaaggttg gctagattgt actcagacaa ttctcccttg acttgtaaat tttcattgct
    26161 gacaatatga accataactt gactcgcccc tgttggagat ttagaaacat tgctattgac
    26221 ttcgactaca gttccctcta gggtactgag aacagttgtt gcatccaatt gactttgagc
    26281 cttgcttaat tgcgctgcag catctgcacg cgcatcacgg gcatcaccca attgagcatc
    26341 aatagaagca acagaatttc cagccactgg agttgggctt tgcaccgttg catcttctcc
    26401 tcctactggc gctggtaact gtggagcctg agctgaagcg gcttcatttc gtgcttgatt
    26461 gagttcattg atatgacgat ctgccttagc tactgctcga ctagctgaat cataggccgc
    26521 ctgcgcttct gaactactgt acttgactaa agcctgccct tcgctgacct tatcgcccac
    26581 agaaacaagg atttcatcta aatcaccctt actagcatca aaataaacat attgttcatt
    26641 ttttgctgtt actgtccctg acaataaaac agaggatgcc acgcttcctt ccttggcaac
    26701 aacaagatga gtaggctcat cttttacagc agtctgagaa ggttgtctaa aaagtaaaat
    26761 cccccggcac ccaatacaac tacactcgca gcaccgattg ctgcatacag ttgccacttt
    26821 ttagctttac cattcttttt cttcataatg aaactccttt tcttttttac aatactttgc
    26881 tattatacca aatttccctc cagcaaacaa tgcagttcag atggaaataa tggcagttca
    26941 ggattaaaca atcgttcgga attttacttt tctattgtac tagatatata atacactatc
    27001 ttgacttatt tggcaagctt ttttgcaatt ttttgtgcgt agcagatttt tgcaatcaaa
    27061 tcaaaaaaag aataaaatat aactatcaaa aaagacactc tactatttaa aagattacaa
    27121 aggagttttc aatgagagaa tatgatatca ttgctatcgg tggaggtagc ggaggaatcg
    27181 ctaccatgaa ccgtgctggt gaacatggag ccaaagcagc cgttattgag gaaaagaaat
    27241 taggtggaac ctgtgtcaac gtcggttgtg ttcctaaaaa aatcatgtgg tacggggcgc
    27301 aaatcgctga gactttccat caatttggag aagactacgg ctttaagact actgatctta
    27361 actttaactt tgcaacccta cgtcgcaatc gtgaagccta cattgatcgc gctcgttctt
    27421 cttatgatgg tagttttaaa cgcaacggtg tagacttgat tgaaggtcat gctgaatttg
    27481 tagattctca tactgtaagc gtaaatggtg aactgattcg tgctaaacat atcgtgattg
    27541 ctacaggtgc ccatccaagt attcctaata ttcctggtgc tgagctaggt ggctcttctg
    27601 atgatgtatt tgcctgggaa gaacttccag agtcaattgc cattctaggc gctggttata
    27661 tcgccgttga attagctggc gtactccaca cttttggtgt caagacagat ctctttgttc
    27721 gccgcgatcg tcctttacgt ggttttgatt cctacatcgt tgaaggtttg gtcaaggaaa
    27781 tggaaagaac aaacttacca cttcacactc acaaagtccc tgtcaagtta gaaaaaacta
    27841 ctgacggcat taccattcat ttcgaagatg gtactagtca cacagctagc caagttatct
    27901 gggctacagg tcgccgtcca aacgttaagg gattgcaact tgaaaaagct ggagtgactc
    27961 tgaacgaacg tgactttatc caagtggatg aataccaaaa tactgttgtt gagggaatct
    28021 atgctctagg tgatgtaacg ggcgagaaag aactgactcc agttgcaatc aaggccggac
    28081 gtaccctatc tgaacgtctc tttaacggaa aaactactgc aaaaatggat tactcaacta
    28141 ttccaactgt tgtcttttca caccctgcta tcggaactgt tggtttgaca gaagagcaag
    28201 ctattaaaga atacggtcaa gaccaaatca aggtttataa atcaagcttt gcatctatgt
    28261 actctgcttg cactcgcaac cgtcaagaat cccgtttcaa actcataaca gctggttcag
    28321 aagaaaaagt tgtcggactt catggaattg gctacggcgt tgatgaaatg attcagggat
    28381 ttgccgttgc tatcaaaatg ggagcaacca aggctgactt tgatgcaact gtggcaattc
    28441 acccaactgc atctgaagaa tttgtaacca tgcgttaatc gaaacaaaag aagctgatat
    28501 aaatatgtct gcttcttttt taatgatttt cgaatcaaca aaaaaattat ccaacttgac
    28561 tagtcacgaa aatcggctgt tacagttttt gttaccttgt ttttaaaaaa tggttgacat
    28621 cgactcctat atgagtataa tagttactat acattagaaa agaggttaac tattttgaaa
    28681 aaagctcacg tttatgctat ccctgctatt ggggctgctc tcattgctgc tttggcacaa
    28741 atcagtcttc caattggacc tgttcccttc actctgcaaa actttgcaat tggcttgatt
    28801 gctactgtct ttagaccgag agaggctata ctttctgctg gactctatct tcttctaggt
    28861 gctatcggtc ttcctgtctt tgcaggaggt ggagctggtt ttcaggcttt agttggccct
    28921 actgcaggct atctttggtt ttatctcgtt tactctggac ttacttcctc tctaaccaac
    28981 agcaagagtg gtgttgttaa gatttttctt gcaaacctct tgggtgatgc ccttgtcttt
    29041 gtcggcggga ttctcagctt gcatttccta gctggaatgg catttgaaaa agctcttgct
    29101 gtgggggttc ttccctttat cattccagac cttggcaaac ttctggctat tagttttatt
    29161 agccgtcccc tacttcaacg ccttaaaaat caggcttact ttactaacta aaaaaggata
    29221 tcgagttatc atgactcaat atccttttct tttattttga aaacttatac tcaatgaaaa
    29281 tcaaagagca aactaggaag ctagccgcag gctgctcaaa acactgtttt gaggttgtgg
    29341 atgaaactga cgaagtcagt aaccatacat acggcaaggc aaagctgacg cggtttgaag
    29401 agattttcga agagtattag ttgcctttaa aggcatccac catcgtttga aattcttcat
    29461 ttgagagagt aatccctttg cccattttag tatggtctgg actccaagca cgaatatcaa
    29521 actttgcagg ggcaccatta aagctcacac ggttaatttc cttggtccaa cctttttcgt
    29581 tttcagaaag agtcaacaag tgctcttcga tttcaaatgt aaattctgcc attttcttct
    29641 ccttttttag tttcattagt ttattcgtaa aatcttgtag attttaggaa aattttatat
    29701 aatattgata taaaagaagg gaggccaata tgagacataa attccagcaa gttctaaata
    29761 aaatacatga ttttttaaat ggatatgacc aacctgacca gactgaaacc aactccctta
    29821 cagccactat tgaagaggct atccagaaac aaaccgctgt tcaccttatc ttgtctgaga
    29881 caagctttac aggtgacatc atcaaatatg atcagcaagg ccagcaaatt atcgtgaaaa
    29941 atttttccaa aaatgtgagc cggattatcc gtataagcga tattcaacgc ctgcgatttg
    30001 tcccctcaac tgtccaaaca gcccaaaaaa atagatttaa gaaagagtga gatgtagttg
    30061 cttcatccca ctctttttct tagcgaattt gttcaaaatg taaatgaact gcaatatgat
    30121 ctccataacc acttctttcc aagtcacgtt gtaaacgata ggaaatgtag tgttctgcaa
    30181 tggtaatgta acctgcgccc aataaacgat gttcaaccat agattgaatc atactgatag
    30241 tcgcacgttc caccttggct tcttgtaaat ccaaaactac cttcttagtg acttgagcaa
    30301 gattttgacg caaatcatct gtcaaaacat aaacagtttg ggctgccttc aagatggctt
    30361 ggtaaatctt atctggatta aattcagcaa tttcgccatt acgtttgatt acttgcatag
    30421 gtttctcctt tattctttgt tttctttgat ttctgccagc attttttctt cttctactgt
    30481 cagttgataa tgttcaagta aatctggtct gcgctcgtag gttttcttta aactctcgta
    30541 caatcgccac tgacgaatct tttcatggtg gccactcatc aatacatctg gcacgaccat
    30601 gcctcgataa tcatagggac gtgtgtactg aggatattct aaaagacctg aagaaaaact
    30661 atcatcttgg tggctagact ccttgccaat cacttctgga atcaggcgaa ctgtagcatc
    30721 aatcatggtc atagctgcca attctccacc agtgaggaca tagtcgccta gggaaatctc
    30781 atctgttacc aaggtcttaa tgcgctcatc ataaccctca tagtgcccac agataaagat
    30841 tagctcttcc tcttgagcca aatcttcagc ataagcctga tcaaactgct ttccagcagg
    30901 atcgaggaga ataacgcgcg gatttttctt ttcaatagca tcaaaggaat tgaaaatagg
    30961 ttgtgctctg agcaacatgc cctgaccgcc tccgtagggc tcatcatcta catgacgggc
    31021 cttttcagca ttttctcgaa aattatgata ctggatatcc aagagccctt tttctcgagc
    31081 ctttccaacg attgagtgct ccagtggaga aaacatctct ggaaagaggg ttaaaatatc
    31141 aatcttcatc gtctaaccct tctaagattt ccacatcgac ccgtttattt ggaatatcaa
    31201 cattgagaac cactggtggg atataaggta aaagcaaatc acgtttgcct tttcgtttga
    31261 ccacccagac atcattagca cctggttgca ggatttcctt gatggttcca accaagctat
    31321 caccctcata gacttccaaa ccgataatct cgtgatagta aaattcacca tcgtctaggt
    31381 cattcaaatc ttcctcagcg accttgagac tgtatccctt gtacttttcg atagtattga
    31441 tatggtacat atctttgaat ttaataatgt caaagttctt ctgtttacgg tggctagcga
    31501 tggtcactgt ttggacaaac tgatcttttt catcaaacaa agccagctca gctccttttt
    31561 taaaccgttc ttctgcaaaa tccgtcacag acaagactcg catctcaccc tgtaatccct
    31621 gcgtattaac gattttccca acattaaagt agttcatctt gtctcctgta atctcctttt
    31681 ttccatctta ttctgacaat tctcgaataa tagccgcaat tttttccgat tctgaccatt
    31741 gtaaataatg gtgattcctt cctaaaatga gtttagtatt ggaagtccaa tattctgatt
    31801 ctctgtactc tttttctcta taagtctgac aaaaaataaa tacagggaca tgagattcta
    31861 tcgacaaatt ctcaaaatct tcctcagtaa tctctccaga tatctgaaat tctggatctt
    31921 gattttccaa ctctaagcct ttttcttgca ttaattccca gattttttta ttcgtttcag
    31981 gactaaatgt tgcttgagtt aagttcttaa aataaagttc aggaccacac tcgtcaatca
    32041 gcctcatctg ctcttccatt tctggataag gattttctga aaaatcagca aacatgactt
    32101 ttttagttgt cggttcaatt gctactaaag tctgacgctt aattggtttc tcgagtaatt
    32161 tgcaagctaa aattccactc caactatgtg cacaaagtat atattcagaa attcctaatt
    32221 cttcaagtac ttcataaacc gcatctgcaa gattatctag attttttcca gcttggtcat
    32281 gaatcggact cctacctgtg ttcggaaaat caattgtcaa ataaccaatt gtaggaggag
    32341 gtttttcaag tataagtgaa aaattttcat aacttggtag caaacctgct ccgtttagac
    32401 aaactagcac tttcttttgc ttttgataag taacagagag gctaccaatt tctgtagata
    32461 cttcaaacct cttcataaag aaatccactc attctatata atgaattatt aaaaatcctt
    32521 atcctttatt ttatcacgtt ccaaagattt tctcaagttg gaggaagggg acaacatctc
    32581 tactttccct tcaagaatcc ttccaaatca tgtttatgtt ggtaattaac ggctgcggtt
    32641 ttgtctttct caaagatggt tctagttagg tcaatatgct tgatagcagc aatagcgacg
    32701 gtgtagtgaa tgatatcagc cagttctatg gcaggttcct cgttcgaatc ctatcccttc
    32761 ttttcgacca gagcgcctat tcaaaacctc ggctacttct ccgacttcct ccactaactt
    32821 cataaagaga ccttcatcag tccgagactg ctgttaatgt tcgagtaagt agtcttggaa
    32881 ttgcctaaac gttcaatctt ttatagtata ttgaaactag aatagtacac ctttacttct
    32941 aaaacattgt tagaaatcga tttgactgtc ctgatcgatt tgtcctgtta ttatttcatt
    33001 ttactatatc ttctattcca cacaaaaaag cgagacatcc gtcccgccct tcttattttt
    33061 cgtcaataac gattcttact tttttgtatt cagttgggac agagtagaca atcgttctta
    33121 tcgcagaaat agtgcgaccc ttacgaccga ttacacgacc cacatcgctt tgatcaagat
    33181 tcaaatgata ttccaaaaat tctggtgtat cttcaatctt gatagttaag gcatctggtt
    33241 gtgaaattaa gggtttcaca atcgcaataa tgagattttc aatcgtatcc atctgtcaac
    33301 ctactttaaa cttattttga gaatttagaa tcgtggaatt ttttcaatac gccttctttt
    33361 gaaaggatgt tacgtactgt gtctgaaggt tgagctccat tagccaacca tgcaagaacg
    33421 cggtcttctt tcaaagttac ttggttttca gcaacaagtg ggttgtaagt tccaactgtt
    33481 tcgatgaaac gtccgtcacg tggtgaacgt gaatctgcta cgttgatacg gtagaaaggt
    33541 tttttcttag aacccatacg agtcaaacgg attttaactg ccatttttaa agtctcattt
    33601 ctttaatttt ttatttcggt gaaatagctg agctatttag cacatgttct attatagcag
    33661 atttttggca tgtgtcaaga aaaaaacttg acagactata aatttcttaa agtatttaga
    33721 aatttgttag aataggaaat aaatgtttga aagatactat ccgtgaatac tattttagaa
    33781 aaattttata aagaacacca agtcaaaccc ttcatatcgc ctgaacgaga tttggatact
    33841 tggctcctaa gtcccaagcc cgttcccaag cgaaatatga acttattagc agatgattca
    33901 ctagcaggag atatcatttt actatggaga atccagtttg gtacttttac cactgaaaca
    33961 tgattttaaa aactcccatt cgaatatgat tcaaaattcg cttagaatca atgttttccc
    34021 gctttttcaa aaaataaatt ttcatctaat ttaaataact ttcaattaag tgtgaatttt
    34081 gcttaaaatc attaaaaagc accccatggt gtgaaccatg aagtgttgta aatgctgtgt
    34141 tgtagctagt tcttagcgtt tagagaactg gctagcttta cgagctttct taagatctgg
    34201 tttccaaaat actttgagta cacttatggt attgatgccc ctaaacatct aaaaactctg
    34261 gtagaaaaag ggtatgctct tgtcgaaact tcttttgact cacttgacca tctaaatact
    34321 accatgaaaa agaatatcct gaagaagaag ggagttactg gactttctaa aatgaaatct
    34381 tgcgacctgg atcaagccct tcatgagcat ttttcagaag aagaattagc tggtcacttt
    34441 catgtccttc tatggacttt tttacaatgg cattgctatc acacccaata cctatctaag
    34501 cgcctggttc gtaaacttta ttgcagctct tcctctaaat ttcctaattg ttgaaccaat
    34561 tgcccgtttt atactaagtt cttttcagaa accatttact ggggaagaag ttgaagaagt
    34621 tgaagatttt caagatgatg atgaaatccc aactattatc taagccagtt ctgtaaacta
    34681 ctaatatttg aaatccactt ccttttaggg tacaatggta taaatgaatt tttgagagga
    34741 tcagaatgaa aaaactagca acccttcttt tactgtctac tgtagcccta gctgggtgta
    34801 gcaacgtcca acgcagtctg cgtggtgatg attatgttga ttccagtctt gctgctgaag
    34861 aaagttccaa agtagctgcc caatctgcca aggagttaaa cgatgcttta acaaacgaaa
    34921 acgccaattt cccacaacta tctaaggaag ttgctgaaga tgaagccgaa gtgattctca
    34981 acacaagcca aggtgatatt cgcattaaac tcttccctaa actcgctcct ctagcggttg
    35041 aaaatttcct cactcatgcc aaagaaggct actataacgg tattaccttc caccgtgtca
    35101 tcgatggctt tatggtccaa actggagatc caaaagggga cggtacaggt ggtcagtcca
    35161 tctggcatga caaggataag actaaagaca aaggaactgg tttcaagaac gagattactc
    35221 cttatttgta taacatccgc ggtgctcttg ctatggctaa tactggtcaa ccaaacacca
    35281 atggcagcca gttcttcatc aaccaaaact ctacagatac ctcttctaaa ctccctacaa
    35341 gcaagtatcc acagaaaatt atcgaagcct acaaagaagg tggaaaccct agtctagatg
    35401 gcaaacaccc agtctttggt caagtgattg gcggtatgga tgttgtggat aagattgcta
    35461 aggccgaaaa agatgaaaaa gacaagccaa ctactgctat cacaatcgac agcatcgaag
    35521 tggtgaaaga ctacgatttt aaatcttaaa aaccaaaaaa atacagtatc cacattcggt
    35581 actgtatttc ttttactctc attcttaagt taaattatta aaatcccata tttggtctat
    35641 ccaaccttca taaaagtctg gatcgtggca gaccataagg atagatcccc tgtattcttt
    35701 gagagcgcgt ttgagctcat cctttgcatc cacatccaaa tggttggtcg gctcgtccag
    35761 cactaaaacg ttgttttcac gattcatcaa gagacagaaa cgaaccttgg cttgctctcc
    35821 ccctgataat acttgaatct ggctttcaat atgtttggtt gtcaaaccac aacgggcaag
    35881 ggctgcacgg acttctgctt gattaagggc aggaaaggca ttccagacag cttcaagagg
    35941 agtttggcga ttaccgcctt ctacttcctg ctcaaaataa ccaagttcta aataatctcc
    36001 acgctccact tccccagcga ttggcgagat aatgcccaag agactcttca agagagttgt
    36061 ttttccaata ccattagcac caataatcgc aaccttttga ttgcgttcga aggtaagatt
    36121 taaaggctta gtaagaggac ggtcgtaacc aatttgcaag ttcttggctt ggaagataaa
    36181 gcgccctggt gtacgagctg gtttgaaatc aaaggatggt tttggtttct cactttggag
    36241 ttcgataata tccatcttat ccaatttctt ttgacgagac atagccatat tacgagttgc
    36301 aacacgggct ttattacgag ccacaaagtc cttgaggtct gcaatctctt tctgctggcg
    36361 ttcgtaggct gcctctagct gagatttctt catagcataa acttcttgga actggtagta
    36421 gtcaccagag taacgcgtca gctgttgatt ttccacatga tagacaatat tgataacgtc
    36481 attgaggaat ggaatatcgt gcgaaatgag aacaaaggca ttctcatagt tttggagata
    36541 gcgcttgagc caatcaatat gctcagcatc caagtagttg gtcggctcgt ccaacagcaa
    36601 aatatcaggc ttttcaagga gaagttttgc caaaagcacc ttggttcttt gcccacctga
    36661 caaagaagtt acatccgtat ccatgccaaa gtccataata ccaagagcac gcgctacttc
    36721 gtcaatctta gcatccaagg tatagaaatc acgactctcc agacggtctt gaagttctcc
    36781 cacttcttcc atgagagcat caacatccgc accgtcttca gccatttcca tatagaggtc
    36841 attgatacga gcttcagctt tgaaaagctc atcaaaagcc gtacggagaa catcacgcac
    36901 cgactgtctt tcagcaagga cagagtgctg atccaagtaa ccagccgtca catatttgga
    36961 ccactcaacc tttccttcat ctggcagcat tttaccagtc acgatactca taaaggttga
    37021 ttttccttca ccattggcac cgaccaggcc gatatgttct cccttgagga gacggaagga
    37081 cacatcttca aaaattgcac ggtcaccaaa accgtgactc agatttttaa cttctaaaat
    37141 actcatttta attccttatc ttgtttttat gtaatcgttt ataaaggagc caagacagat
    37201 agccacccaa agtgttggtc cacaaatcat caatctcaaa gacgcgattg aaatcaaaga
    37261 aaaagtccaa gattaattgc gtacactcga ttccaagact cacaagaaaa ctaaaaagaa
    37321 ggaccttttt tgttttccgc aaatttggaa atagataaag gagttggaaa atcagaggaa
    37381 aaaacaagaa gacattgagg atattttgta aaaaaatcca acataattgt ccaatgtcac
    37441 tcacttcgcc cagtttccag agagaattga aaggagtcaa aagaaaaacc aggcgtccaa
    37501 gatgctgaat atctggagtt cccactccca cggtagattg ttcttgagga gtaaagcaaa
    37561 aatagacaat gcaaatgcta tagaaaatga ctccccagac caaaatatga ttataagtct
    37621 tcttcatcat taaggattta ccgctgcgac taccttctgg cggtcacgtt tcattgtgtt
    37681 aaagcgcaat tgtccacaag ctgcgtcaat atctgtacca tgctcttgac gaaccacaca
    37741 gttgccccct tttttcttaa gcgtatcata gaaagccaac acgcgctctt tgggactacg
    37801 gctatattgg tcatgctcac taactgggtt ataaggaatc aagtttacat aagacaattt
    37861 cttgatgttc ttgagcaatt cagccaattc caaggcttgt tctacaccgt cattgacttc
    37921 attaagcatg atatattcaa aggttacacg acggtttgtt gtctcaatgt agtattcaat
    37981 agcagcaaag agtttttcaa tcggaaaggc acggttaatc ttcatgatac ttgaacgaag
    38041 ttcattgttg ggtgcgtgaa gagacacggc aagattgacc tgaacccctt catcagcaaa
    38101 gtcacgaatt ttatgagcca aacctgaggt tgaaaccgtg atgtgacgag caccgatagc
    38161 cattccttta tcatcattga tagtacgaaa gaaattcaag acattgttgt aattatcaaa
    38221 gggctcaccg attcccatga caacgatatg gttgacgcgt tcatcctgac cacgctcatc
    38281 aaagtatttc tgaaccagca tgatttgcgc tacgatttca ccgttattga ggtcacgctg
    38341 cttcttaatc aaaccagagg cacagaaggt acaaccgata ttacagccga cctgagtggt
    38401 cacacagaca gataaaccat agtgttgaca catgagtaca atctcaatta acataccgtc
    38461 aggcaattca aagagatatt tgactgtacc atcagcagac tcttgcacaa tacgttgttt
    38521 caagggattg accacaaact ggtcattgag cttagcaatc aaatccttgg aaaggttggt
    38581 catttcttca aatgactgca cacgtttacg gtagagccat tcccagattt gatctgcacg
    38641 gaatttcttt tctccctgct ccaataccca ttcctgcatg gtttgatgtg ccaaactatg
    38701 aattgagggt ttcatttctt ctccttattc tctactcact tctgacgaat gacaaaatga
    38761 cgttgttcct tgtcctcttt ctgagaatgc tggtctttct gacgacgtct atttttctta
    38821 tctgcattcg actttcgttt agtttgagtc ggtttctttc cttttctaga aggtgtttct
    38881 tcttccgtct tacgcatttt cttgtcaaat gatgctcgct taggggcttc attttctaag
    38941 acaaaatagg cacaaccata actacaatac tctaaaaggt agtcttgtaa acgactgatt
    39001 ttttcaagtt tttcttctgt tcggtcatcc ttgtaaaaac ctcgtaggcg aagctgttcg
    39061 ttgctccagt cccccacgat ataatcaaac ttggttaata cttctgaaaa acgctgatta
    39121 aaagtcgtca catcaaaggc atccttgata ttttcaacca aggaaaaagc tatcccttcc
    39181 gtttcgacct tgtccccgtg taaatggaac tccggaccag gaaacttgtt atagttgtat
    39241 aattcaggtg caatttcttt tcgcatagat atcctttttc cacgattact taatacttta
    39301 ttctaccata atttctagca gttagcacgt ttctcataaa aatgaaaaaa gtctgacgat
    39361 tttgtcagac cagaatctta taacctaaaa aagagaagaa caattcttcc ctccaactat
    39421 cattatttag cagctgcgta caattcatct actttattcc agttgattac tgaaaagaaa
    39481 gctttgatat agtcaggacg cacgttgcgg tatttcacgt agtaagcatg ttcccaaacg
    39541 tccaagccca agattggttt tttaccttct gagattggtg tgtcttggtt tgctgttgaa
    39601 gtcacttcaa gtttcccttc tttgttgaca accaaccatg cccaacctga accaaaacga
    39661 gttgttgctg ctgcagtgaa ggctgcttgg aattcttcaa atgaaccaaa tgttgcatca
    39721 attgctgctg ccagttctgc tgaaggagct gttttctcgg gagtcatcaa ttcccagaaa
    39781 agagcgtggt tcaagtgtcc gccaccattg ttgataagtg cttgacggat atcagctggg
    39841 atagattcta catcagcaag caaggcttca aggtcttcac cgatttcagg gtgtttttct
    39901 aaagctgcat tggcattgtt gacataagtt tgatggtgtt tgtcatggtg caaatgcatt
    39961 gtttccgcat cgatgtatgg ttccaaagcg tcgtatgcat atggaagttc tggtaagata
    40021 atagccatct gtaatacctc tttttctttc tatatgaaaa tgataacgca aacattcact
    40081 tttttcaagt tttttgctta tagataagga aatcactgac atactttcta ataaaataag
    40141 caaatcagca agaaacccac gacaagataa tcctgacgcg ggttgtagtt tcaacaaatg
    40201 tcaattgacc tgactagcaa tctgtaagag tgccttttca aaaaggaaac ctttttcata
    40261 aagacctgtc ttaatctgat agtctgtctc aatcaaatag gaaatagctt gcttcagaaa
    40321 gctcaaagaa agtcctctcg aatctcttaa tgcaaacttg atttgataag gatttgggtt
    40381 acgtcccaga taactaccta aactacttgc aatctgcgat tctgtttggc cagactctgc
    40441 caaaatcttc acctgagtaa aagtccgaaa ttgtcccagc atgactgcaa tcagtttgat
    40501 ttcatcttcc ccttgcaagg tcaagtctct caccaaatcg cgcgcctgat ccatcttttt
    40561 agtcagaata aactgagtta aatcaaaaat attgtcctgc aaggtcttgg gaattgcgtt
    40621 aacaatatcc tcttcctcga taacagaatc cgccttatag gactgtaaaa agagaagatt
    40681 tttctggatt tcgctaaatt gaaaccccga cttgatgagg agattttcaa aagaatgatt
    40741 ggtaaactgc agaccttgtt tctgactcca cttttggaaa tactggcgca attcttgttc
    40801 ttttacttct actgcatcga agaccttggc atcacgctta agtaatttga ctaaccgtct
    40861 tttgctatcc agctttcctt ctgcaaagat tatcaacttg gttgttggag aaggattgtc
    40921 aaggtattcc tcaaatgact taagctcatc atctgtcaaa aagcgtttct tagcagtcgt
    40981 gatatccata aaataatcta atatcacgat tttctcatcc gcaaagaaag gaaggctgac
    41041 caactccagt tccacatcct tgtaaactac ttctttcata tcaaagtagg caaagttgag
    41101 gtcagcagaa tcatacccaa tctgtttcaa cacttgactc ttcatcactt caaactgacc
    41161 ctgatctgtc cctgtaaata ggctcaggct cggtaaattt gataaagtca acttctgact
    41221 ttcttcaatg gctagcatcg tctctccttt cttcagattt ttcgatttaa tttagtcaat
    41281 atagcgcaat ttcccacgga aatcttctaa gctctcgtag cctttttcca ccatgattgc
    41341 tttcagttca ttggtaatgc ggtcaaaagc actgacgcct tctttgtgaa gggtcgttcc
    41401 cacctgcacc atacttgctc cacagaggat gtgttcaaag gcatctcgac cagtcagaac
    41461 gccacctgtt ccgataattt ggatttgagg atttaaacgt tgataaaagg cgtgaacatt
    41521 ggctagagca gtcggtttga tgtattctcc accaattcca ccaaaaccat tcttaggccg
    41581 aataacgaca gattcgtctt ctatatagag gccgtttccg atagagttaa cgcagttgac
    41641 aaacttgagc ggatatttgt tgaaaatagc tgccgcttga tcaaagtgaa caatatcaaa
    41701 ataaggtggc aatttaattc caagaggttt ggtgaagtaa gcaaacactt ctgccaaaat
    41761 ccggtctgtt gtctcaaaat cataggcaat ctgaggttta cctggaacat ttggacagga
    41821 aagatttagc tcagtcagac cacgaaaatc actctcttgg acttttttca aaatagtatg
    41881 ggtttcctct ggagacatgc cgaccagaga taagaagaaa gttcggttcg actctttttc
    41941 ctgcaaatct aaaagataat ccaaataata gtctaagcca ttatttggca agcccataga
    42001 gttgatggaa ccaagtggaa catcttggta gcgtggctca ggattcccct gacggaagtc
    42061 caaggtcgct gtcttagtaa caaaggttcc tgccgctgag tttttgacct cttctaactc
    42121 ctctatcgtc atacaagcca cacctgctgc attcatcaag caattgtcaa actcaaaacc
    42181 agcaatttgt gttttcgttg ataccatgat tctaatcctc cagatttctt ttattgctaa
    42241 tattatacca taattttaat tctaggttta ctagattctg tggttctatc tatttattcg
    42301 gaaaagaaag tctagtacag aagtcatgct atggaaacag ctagtgtatt agaagtttga
    42361 acaaggcttg ggtaacagac ttttgaatga agtccaagca acaaggagaa atctcaacca
    42421 tgagtccatt ccatgactct gttgctctag aaaaccttgc aatagtcaag tgataacgaa
    42481 taaaactttg gattgaaata tccccaagat tatctattac gaggagaaat cttaaccttt
    42541 ataattttta aagaaatata gtaaaatgaa acaagaacag gagaaatcga tcaggacagt
    42601 caaattgatt gctaacaatg ttttagaagt tgaggtgtac tattctagtt tcaatctact
    42661 atacatgtaa atctctactt tcgcaaaatg gattttcaga acagtatccc agtatagtta
    42721 aaaactataa aaaagagagg acgttcccct cttaaaatct tattttactg cttctttcaa
    42781 agcatctacc ttatccaaac gttcccatgg aagatcaata tctgtacgtc ccatgtgacc
    42841 gtaagccgat gtttgacggt aaattggacg cttgaggtcc agcatttgga taatccctgc
    42901 agggcgaagg tcaaagattt gacgagccgc tttttcaagt tgactttcag ctactgttcc
    42961 tgtaccgaaa gtatcgatac gaacagaaac aggttgcgca acaccgatag cataggccaa
    43021 ctgcacttct gccttcttag caaggcctgc tgcaacgata ttcttggcaa tatagcgagc
    43081 cgcataagag gctgaacgat ccaccttagt cgcatcttta ccagagaagg caccaccacc
    43141 atgacgagag tagccaccat aagtatctac aataatctta cgaccagtca aacctgagtc
    43201 cccttgagga ccaccgatta caaaacgacc tgtcggattg ataaagaatt ttgtcttatc
    43261 atcaagataa gaagatggaa taacttcttt gatgaccttg tcaatcacat cttgatggat
    43321 ttgttcatta gtggcctctg gatcatgctg agtagaaata acgactgtat ctacacgtac
    43381 cggacggtca ttttcatcgt actcaactgt aacttgtgat tttgcatctg gacggagata
    43441 gctaatttct ccagacttac gaagttctgc cagacgacga atcaatttat gactgagtgc
    43501 aattggcaat ggcataagct cttctgtttc atctactgca aatccaaaca tgagcccttg
    43561 gtcacctgct ccaatcaagt ccagtggatc ttgatcagca tttccacgaa cctccaaggc
    43621 ttcgttaaca ccttgagcga tgtcaggaga ttgttccacc aaagatgggt gtactcccac
    43681 cgtctcagca gaaaatccat attctgtatt ggtataacca atctctgcaa tggtatcacg
    43741 aaccacacgg ttaatatcca cataggcatt tgtagaaatt tcaccaaaaa cgtggacaga
    43801 accagtatat acagctgttt cagcagcaac gtgcgcctct ggatcctttg ctaaaatagc
    43861 atccaaaatc gcatctgaaa tttggtctgc aatcttatcc ggatgcccct cagatacaga
    43921 ttcagacgtg aataatttac gctctgacat aaaaatgtcc ccccttaaaa aatattgtta
    43981 tagagttaca aagtactgat aggaaaactc ttcgtaataa atacctacca tcttatcggg
    44041 actatataac tttatcatta taccattttt aatcgcaatt tgcagtcttt aatgtagtat
    44101 ttcggtttag atagttcgtt ctcataaagc aaaaagttgg agctcagtac tccaacaatt
    44161 tctattaaaa tgtttgacgt ttagctttac gctctgcacg accgcgagcg cgattttcag
    44221 cacgcttggt tttacggcgc ttttcatcaa ccgcccattg aattttcttc ttataacccg
    44281 gtttgacttt tttctttttc tttttaacca aaccaatcat ttcgatatca agtttatctt
    44341 gttttttctc acggttggca cgacgatcac ggtcataggt atcttgaaat tccccgtctt
    44401 tgaccatctt aggactaaac ttgattccca atttctccaa ctcacggata tccgagtcat
    44461 cacttggctg ataaagggta atagctgtac ctggtaggcc atttcgtcca gtacgaccaa
    44521 cacggtgaac aaaaaaagat aagtcttgcg gaatggcatc attgatgaca tggctgacac
    44581 cttcaatgtc aatcccacgt gctgccaaat ctgttgcgac aatatactca aaatccagat
    44641 tttgcacctg attcatgatt cgcttgcgtt cacgaggggc aatatcgcca tggatttttg
    44701 caaccttcaa gccttgagca gtcagatatg aatgcaattc atcagcacgc gttttagtgt
    44761 taacaaaaat cattgccaaa tacggctgca tcaactgagt caactggtaa atttgagcat
    44821 tcttatcatg tcccttggtc gaaatcaacc aattatcaat ggtgtcagaa ataaccgttt
    44881 tggtcttaat tttctccata acaggattgg ataagtattt tttcaagaat ggttgcagtt
    44941 tttgtgggat agtcgctgag aagaccatga attgcaagtc ttttggaaga ctgccagcaa
    45001 tcttatcaac agtttccaag aatcccatat ccaaggtcat atccgcttca tcaacaacaa
    45061 atgtcttggc tttatgaata gctaaatcac cagatttaac caagtcgtag atacggcctg
    45121 gtgttccaat aacaatatga ggctgattgc ttgccaattt ctcaatctgg cgagccttat
    45181 ccgtaccacc cacataatta accacacgaa cttcgacatc tgagtgagct gaaatctgac
    45241 gcgctacttg gtaaatttga gtagccaact cacgactcgg tgcagtaatc actgcttgta
    45301 cactatcgct agcttcatct aattgctgga aaatcggtaa caagaaagta tgagtcttac
    45361 ctgaacctgt ttttgattct cctactaggt cacgacctgc caaaacaata ggaatcaact
    45421 tgtcttgcac ctctgttgga gttgtaaatt ttaactcctc caaggcttct ctaatatagt
    45481 ttttaaattg aaatttcgta aatgacataa catcctcgat tctatctatc ttatcaatta
    45541 taccatattt tattccatta cagtagtctc acttatttag gctatttcca gtagcttctc
    45601 tagtaagaaa aggctggaat tttatagttc caacctcttt tcagttatta tttccagttt
    45661 aacatagcat tcaagccata gtgatcactc acttgtggac tcttgttacc atcaaatacg
    45721 acatgtaaat tttccaccgc taactctttg gtagtaaaga cataatcgat tcgaaggggt
    45781 tcagtgttcc ctttccagcc atcaatttca ggcggaacag tatagctacc acttttctct
    45841 tgagcaactt caaatgcgtc ttgtaagcct aatggactag ctaaaatagc ttggtaacct
    45901 tcctgacctg ctgggttgtt aaaatctcca gctagtaaaa gtggcttgtt caattttttc
    45961 aagacagcct caaatcgtgc ccattcttct tgaaaacctt tatcccacca agagagatga
    46021 acactggcaa ctgctagctc cttgccatcg actacagttt cagctagggc aacacggcga
    46081 gtatgatagt ctgttggatc atccacatct gaaaccaaaa tttctctggc ttcaataggt
    46141 gttttagaca agatagccac accttcgtgg tagcggtcat agccgatatg gttataggcc
    46201 caggtccagt agtaattttt cccttgctca gataactttt caaccaagag tctaacatag
    46261 tgatcttggt gaataggctc agctgctggc aaagcttgat aaaggtcatt aacctccacc
    46321 tctgacgagg tcatctcctg attaatttct tggaaacaaa tcaaatcata gtccttttca
    46381 agaatatctt caagcaaaat ctggaatttt tcctctgctt ctttctccat ccaactatga
    46441 gtattgagtg ttaaaaattt cattcttttc tcccaaaata agaggttgga atacagcttc
    46501 caacctcaag tatattacaa ttctacttta gcaactgctg ttttagctgc aagagaacct
    46561 gttttttcta atttaactga tttaattgca tcaccatttg tgaagacaac tactgttgaa
    46621 gtttcacgtc ctgctgcacg gatagcattc aagtcagctg taacaaggag atctcctgct
    46681 gcaacttttt gtccttcagc aacatgaact gtaaatggtt taccttcaag acttactgtg
    46741 tccaaaccaa tgtgaaccaa tacttcaaga cctgcttccg tcacaatacc aaaagcatgt
    46801 tttgttggga agatgcttga tacagtacct gaaactggag atacaatgtt tccatttgca
    46861 ggttctactg caaatccatc acccatcatt ttttgagcaa atactggatc ctttacttgt
    46921 tccaaagcaa caacttgacc gtctgctact gagtaaactt cctcagtaag atctttgaag
    46981 tgaacagtgt tttgttgtac ttcagtcatt tggcttggaa gagtttcagg aatgatttca
    47041 cctgaatcaa ggatatcttg gatatcagat ttcaaaatgt cagcttttgg accgtagata
    47101 gcttgaaccc cttgtccttt catcacaaga cccatagctc cttctgcttt ccattgctct
    47161 gcatttccta ctttatctgc atctttaaca gttacacgaa gacgagtcat acatgcatca
    47221 acatcaacga tgtttacacg tccaccaaga aggttgataa tgtttacagc ttgagagcct
    47281 gctgcaactt tcacttcgct gctggtttct tctgaacctt cagcagtttc gtagtttccg
    47341 ttgcgccctg gagttgcgta gttgaatttt tgaatcatga agtttgcgat aaagtacatg
    47401 attacagcaa agagaacagt tacccaaacg aagttaacga tatccatacc aataccagca
    47461 ctgattgcaa taggtgtacg agtcaagaac tcgattgaac cgaatgagtg catacgtagg
    47521 tttacgacgt cagccatagc gaaggcagca ccttgaacaa gtgagtaaac aagatacata
    47581 ggtgttgcga tgaacatgaa catgtattcg attggttcag taacccctgt caagaatgtt
    47641 gcaagagctg ttgcaatcat catacctttg tatttatgtt tcttgtcagc atcaacatta
    47701 cggtagatag caacaatcac acccatcaag ataccgaatg aaccgatcat ttgtccaact
    47761 ttgaaacgag ctggatgtac tgtatctaac aagtgttggt attgactagc atcagtacct
    47821 ttaaggttta caaggtctgt tacccatgca agccatagtg ggtcttgacc gaatacttga
    47881 gtacctttag ctgcaccagt taaaatgtca taagtaccac caagagctgt gtagttcatt
    47941 gggatagtca acatgtggtg aagaccaaat ggcaagagca aacgttccaa agtaccatac
    48001 aagaatggtg caagaattgg agcagtttct tgtgagttgg caatccagat accgaaatta
    48061 ttgatacctg tttgaactac tggccagaaa gcagcaagta gaattgcagc gattgctgaa
    48121 cgaagaataa ctacaaatgg tacgaaacgt ttcccgttga agaatgaaag tgcatcagga
    48181 agtttacgga agttgtagta tttgttgtaa gcagttgccc ctacaaaacc tgagataatc
    48241 cctacgaata cccccatgtt caaggctgga gcttcaagaa cactgataaa gtaatcagca
    48301 actttgattg aaccaccaaa gaaagtagtt accatagcat ctggattttt caacatatcg
    48361 cctgatacac caaagattgt accagtgata cggttaatca agatgaaggc aagaccagcg
    48421 gcgaaagcac caccagcacg ttctttagcc cagcttcctc caatggctag ggcaaacaaa
    48481 atgtgaaggt taccgataac cccccaaccg atttgctcaa gaattccacc tgtgatgaca
    48541 agtggtgcaa aggttgggtt aatcatcacg atagacttac cgattgaaat catcaaacca
    48601 gcagccggca taaccgcgat aactaccatc aaagccttac cgaatttttg ccaaaattcg
    48661 aaagacaaga catttttgaa tgtatctttc atcattcaaa tctcctttat ttttatttag
    48721 gcaaacgttt tacgaaaacg tttgcacttt ctatgattta attataccac tttaattttt
    48781 tttgtaaagg cttttataaa aaaattacac ttttttctaa aaaatttcta gtgcgccaaa
    48841 tttcaagttc aaattagcca tcaagaagtc ttctctgggg gacttctttg gaagctcttt
    48901 tcttctgcgc atctcttttc aaaaactctt gtagcaaaaa aggagatttt acaatctcct
    48961 cataaaagca gctattttac ctaaatcttc aagaatcggc gcatggatat tcctgatccc
    49021 aatgaaccaa tgaaaacccc aatcacaaat agtagggcaa tcatcaacgg actaaataaa
    49081 tctggactaa tcatggatag attttgccct accaacgatt tgttgacaga ttggtaaaca
    49141 atttgataaa caataaagac caaaacagat ggtgcgatag ctcccaataa accgataaag
    49201 gctccttcta acaagaacgg tccacggata taactgtttt tagctccgac caagcgcatg
    49261 atttgaattt cgcgactgcg ggaaataatg gtaatacgaa tggtatttga aatcaagaaa
    49321 actgcgataa aaattaacaa agcggcaatc cctagtcccc aaacacggat aaatgaagct
    49381 aacttgaaga gtctttctgt attggcaccg ccatcttgaa cctcagagac accttcaatt
    49441 tttttagcat cttcggctat agtttttaca tcatttggag tgtttgcctc tacaatatag
    49501 gcatcataga gaggattggc atctccttca aagattttcc agttatctcc cattatctcg
    49561 gttaattttt catattgttc ttctttactt gaaaaggtaa cacttttaac cgtagacatg
    49621 ttcttcaaag aatcatatac cttgtggtag tcattatttg taacagtttg accttctttt
    49681 tcaattgtct gactattatc ttccacatcc tttcggatat aaactactac acggacatta
    49741 ttttcaatat ctgtagctag tttcgctgta ttgaaaataa cagatgcaaa tattgccacc
    49801 aaggtcaaag taatcatgac tgaactgaca gcagctactg tcatccaacc atttcgtttc
    49861 aaacttttta aggcttcaaa taaatggcga aaaaatctac taatcatcgt atccatactc
    49921 tccttttgat tcgtcacgaa cgacacggcc attttcaatg gcaatgacac ggtggcgcaa
    49981 ggtatttaca atctggctat tatgagtcgc catcaaaata gttgttcctt gtaggttaat
    50041 ccgttccaag agattcataa tttcccatga attatccgga tccagatttc ctgttggctc
    50101 atcagctatc aatactttgg gattatttac aattgcacgc gcaatcgcaa tccgctgttg
    50161 ctccccacct gagagttcat ttgggaaaga acgaacctta tgcttcaatc caaccaagtc
    50221 caaaacttcc atcactcgtc ttttgatatt acggcgattt tccccgatta cttccatagc
    50281 gtaagcaata ttttcataga cagttttctt tggtaacaat ttataatcct ggaagacaac
    50341 cccaacacta cgacgtagaa gcgggatatc tttctttttg atcttaacca gattaaaacc
    50401 agcaactgat aggcttcctt tatcgatttt tacttcacga tacagagaac gaataaaagt
    50461 tgacttccct gctcctgaag gtcctacgat gtaagcaaat tcccccggtt gaacgctaac
    50521 cgaaacaccg cgtagagcag tcgttccgtt gtcgtatttt ttaacgacat ctctcatttc
    50581 aataattgac atgtgagttc ctttctatct tagctaattc gccacttgag ataagcatcg
    50641 ataaaaccat ctaggtcccc atccataacc ttatctacct gagcaacctc aaagctagtt
    50701 cggtgatctt ttaccatagt ataaggcgtg aagacataag aacggatttg gcttccccaa
    50761 gtgatctcct ttttctcacc tttgagagaa tctacctctg cagccttctt ctcttgctcc
    50821 atttgataga gcttagcctg caacatcttc atggcacgat ctctatttcc atactgggta
    50881 cgatccactg ttgattggac aacaattcca gttggaatgt gggttaaacg tacacctgtt
    50941 gaaaccttat tgacgttttg tccaccggca ccacctgaac ggaaggtatc catcttgata
    51001 tcatcttcac ggatttccac ttcaatagta tcatccaatt ctggcatcac ttctacagat
    51061 gtgaaagagg tatggcgacg tttggcagag tcaaatggtg agattcgcac taagcggtga
    51121 acacccattt ctgacttgag gagaccatag gcattaggcc cttcaaatga taaagttacc
    51181 gacttaatac cagcctcatc acctgcttgg taatccaaca cttccacttt aaagccttta
    51241 gcattaccat aacgagtata catacgaagc aacatatcac cccagtcctg cgcctcagta
    51301 ccaccagaac ctggatggat ttccaagatg gcattgttgt ggtcataagg ttctgacaag
    51361 agtagagtca tctcgtagct ggtcattatc ttatcaagtt cggctaactg cgctaccagt
    51421 tcatcatgca ctgactcgtc ttcagccaaa aaatccaata aaatttcgac ttcatcctgc
    51481 aactcttcca tcttatggaa ggtattgtaa gtgtttttta attcatttaa ttcttgcgac
    51541 gttttttggg ccgcaatatt atcgttccaa aaatcaggtt ctgtcatctt gttttccaag
    51601 atggcaatct cttcctctag cccttcgagg tcaaagagac cccctgaaag aagctaattt
    51661 ttcacgattt gcgtcaattt tctgacgaat tactgaaatg tccataaata ctcctttttt
    51721 acattatttc attatagcat attttatcat ttctttccat ctttttctct aatccccttt
    51781 tcatgaaaaa agaagccttt gggcttccct tcttacaaga cttttgctga agtgcgagcg
    51841 atttcttcca cttgaatacc atttgcttca aatttttctt tcaaggctgg taaatcaatt
    51901 gatccatcga tttgcacttc gataatcacc ttaccatcct tacgcggaat attgactgta
    51961 tgggagatat tcaaattttc ttctacaatc aaagaaacaa tttttccaag aacaccaact
    52021 tcatcttctg taacaaagcg tacacgaatc ccttcttcgc cataacctgc aatttcaaga
    52081 aaggcttgga aaacgtcacg gtcagtaata actccgtata cttgatggtt atctacgaca
    52141 gggagaatac taatcttatt tttcaacatc agataagttg catcttctag actagcatag
    52201 cctgagacag tgacaacatc gcgaatcatg acatctttta cttttgtctt attcagaaga
    52261 taattcatct catagataga aagacttgtt gctttagatg gacttgcttg tgcaatggtt
    52321 ccctcagtca ccaaaccaac taattgatca ttttcgataa caggcagacg gtgcaaacct
    52381 tgctctctca tcaaatctgc tgcatgagat actgttgtat ctggactaat ataaactacc
    52441 ttgcgggtca taaaatcttt aactgccatg agacttctcc ttttctattc ttatccctat
    52501 tatacaatct ttttgtaaat ctatcaaacg cttacatttc tttttcgaaa ttcagaaaag
    52561 tatgaataag ctagcaaaaa gagctctgaa atcgagctct gtgaatgaag gtaagaaatt
    52621 tttcattcga ttttttcaat tattagaaat taagctttca atgtagatgc agatttgttt
    52681 gcaatctcaa tctaccgact aaaaaggtcc cccggactat attgatgatt acttcgtaat
    52741 ctccatctgc cgactaaaac aatccactgg attgttttag ccacctagat atgcttttct
    52801 gacttcttct gatgaagcga gttcttttcc tgttcctgat aggacgattt tccctgtttc
    52861 cagtacatat cctcggtcag agattgcaag tgctttattg gcattttgtt caatcaagag
    52921 gaccgttgtt ccttgcttct gaatatcttg aatgatatca aaaatttctt ggataaagat
    52981 tggggcaagt cccattgatg gttcatctaa aagaagaagt tttggtgttg acatgagggc
    53041 gcgtcccatg gcaagcattt gttgttcccc ccctgaaaga gtggctgcat cttggttctt
    53101 ccgttcttca agacgaggaa agcgtgagaa aaccttcttc aagttagctt gattttcttc
    53161 acgatttttc tttaagaaag ctcccatttc aagattttcc ataacagtca agccaggaaa
    53221 gacgtggcgt ccttctggaa cttgtgaaag accacttgcc acgattttct gagctggcat
    53281 tttttggatt tcttgaccta aaaattcaat ctttcctgaa cttggtcgaa ccaaacctga
    53341 caaggtgcga agaatagttg tcttacctgc accgttggca ccgataaggg aaacaacttc
    53401 tccttcatta acttcaaagc ttacatcacg aactgcttgg atcataccgt aatgcacaga
    53461 aagattttca acttttaaca tagacattag gcttcacctc ctagataagc ttcgataacg
    53521 cgtttattgg tcttaatttc gtctggagtt ccttgagcga ttaaacggcc atattcaagt
    53581 acgtagatac gttctgttac ttccatgacc agattcatat cgtgttcaat caacatgatt
    53641 gtaatcttaa actcatcttt gatacgacga attaactcag tcaattcggc tgtttcctgt
    53701 gggttcatac ctgctgctgg ttcatctaag aagagaattt taggttccgt agcaagggca
    53761 cgaacaattt ccaaacgacg ttgttgtccg taggaaagat ttttagcaag agtctctgca
    53821 tcaccatcta aatcaaagat tttcaacaat tccaaagctt tagcctttaa ttctttttca
    53881 ctcttgtaaa aagctggtaa gcgtaagaaa ctagtaaaaa catgctgttt gtgatggttt
    53941 ccaaaagcaa tcaaaacatt gtctaaaact gttaaatctt taaaaagacg gatattttgg
    54001 aaagtacgtc caagtcccaa agaggcaatc ttataaggtg atttcccatt caaaaggtga
    54061 ccatctaggg ttactgttcc ctcgcttggt tcataaacac cggtcaaaag gttgaaaagg
    54121 gtggttttcc cagctccgtt tggaccgatt aatccaacca gttccccttc gttcaattca
    54181 agagtcacat ctccaacagc tgttagacca ccaaaatgtt tggttaactg ttttacttca
    54241 agtaatgcca ttagttttgt tcctccttct tagatttttt aaagaaacgt gataggctca
    54301 gttcccacgt tccaaggagt ccacctggtc tgaaaatcat taccaatacc aaggccaaag
    54361 cgtaaataat catacgcaca ctagcaacat cttggagaag catattcaaa attcccagaa
    54421 caatagccga aacaatcgca cctgtaatgg aaccgagtcc accaaataca acaataatca
    54481 aaacgttgat tgagttgatg aaggtgtaat ctttcggtac aacagacccg ataaatcctg
    54541 cctgaagtga cccagcaata cttgcagtaa tggcaccaaa gacaaaagcg atgattttaa
    54601 ttttagtcgt attaacccca actgactcag cagcgatttc atcttcacga acagagaggg
    54661 ttgaacggcc aattgggcta cgcaagaagt tcaaggttgc aatggttgta atcacgacaa
    54721 agaagtaaac catttgccaa gttgtaaagt taggaatccc taagatacct gccgcaccat
    54781 ttgtaagact tccaccattg atgataaaga tacggataat ttcagaaaca cccagagttg
    54841 ctaccgcaag atagtccccc ttcaagcgca aggttggaat gccgacaagt aaggcaactg
    54901 ctcctgaaag caaagcccct acaagcatag ctccaaagaa ggcaccgtag gttggtgatt
    54961 tagaaccaat aatagctgct gcataggcac caatcgccat gaaaccagca tgaccaagtg
    55021 aaaattgtcc tgaaaaacca acgattaagt tgagaccaac agccaaaata atattaattc
    55081 caatttgttg taaaatctgt acatagaata gattaagtac tccgactgaa accagtacac
    55141 taatcaagct atagccagct aacaaaagga gtaaccatag aatattaact tttaaatttt
    55201 ccttcatcgt ttacaccttc tctttcacat tcttaccaag gataccagct gggcggacaa
    55261 tcaagatcaa caacaagatt ccataaacaa tggcatcacg gaaatctgac atcccaaagg
    55321 ctgtcgcaaa ggtttccaat agaccaatca caaagccacc aagagccgca ccaggaataa
    55381 ttccgatacc accaagtact gcggcaacga aagatttaag acctggagta acccccatca
    55441 aaggctcaag agagttataa taaagagcaa tcagaacacc agccgcaccc gcaagagcag
    55501 aacccaaagc gaaggtaaag ctaatcgtac ggtttacatt gatccccatc aattgtgccg
    55561 cgtcgctatc tactgatact gcacgcatgg ctttccccat cttagtcttt tggacaatga
    55621 cttgtaacaa aatcatcaaa atcaaggaaa tggccaaaat cattaactgc acatttgtta
    55681 agctaattgg tcccaaatca tatcgaactg tttgaatcgc ttgagggaag gcacgggtat
    55741 tggcaccaac cagatagacc attccatact ccaataggaa agaaacccca atagccgtaa
    55801 tcaaaacagc aatacgagta gagtggcgca aaggtcggta agcaagaaac tcaatcacga
    55861 caccaagaat agctgtcgct agcatagcta caataagcgc tacaaagaaa ttcatttgga
    55921 aagaattgat caagaaataa ccgataaagg ctcccatcat ataaatatca ccatgggcga
    55981 agttgatgag cttgataatt ccgtaaacca tggtatatcc tagggctaac agcgcgtaaa
    56041 cactacctag aatcaaacca tttacgagtt gttggagcat aagattcact ctttctattt
    56101 ataattccga gggttttccc tcactttttg ataggttctt atactcaatg aaaatcaaag
    56161 agcaaactag gaaactagcc gcaggttgct caaagcactg ctttgaggtt gtagataaga
    56221 ctgacgaagt cagtcacata tataatccaa ggcgacgttg acgcagtttg aagagatttt
    56281 cgaagagtat taaatatcga aacagggagt gagtcaaagg ctcattccct atttcaacat
    56341 ttttctatta tggttttaca acttctgctg cttcaacttt accattgttc atggtcatca
    56401 tgtaagcagt tttgactgtg ttgtggtctg catcgaagct tgtttgacca gttacacctt
    56461 caaaatcttt tgttttagca aggttatcct tgatttcacc tgaattttta gcaccttttg
    56521 ctgcgtttgc tacaaggtga actgaatcat aagccaaggc tgcaaatgtt gaaggctctt
    56581 cattgtactt agcacggtaa gcgtcaagga aggctttagc tttagctgaa acttctacag
    56641 tagttgagaa gcctgagata aagtagatgt ttgatgcttt ttcagcagtt gcttgttgta
    56701 caaactcctc accgttgaat ccatcaccac caacgattgg tttgtcaatt cccatgccac
    56761 gcgcttggtt tacaatttta ccagcctcat tatagtaacc aggaacaacg atagcatcaa
    56821 agtctttccc tttcattttt gtaagggctg cttggaagtc tgtgtcacct gctacgaaag
    56881 tttcatctgc aacgatttca cccttgtatg actcgcggaa agatttggca atccctttag
    56941 catagtcact ggcattgtca gtgtaaagaa caactttctt agcatttaat ttttcagaaa
    57001 catagtttga gataattttt ccttggaagc tatcttggaa agttccaata aagaggtaat
    57061 cttgaccttt agtcaatcca tcttgagtcg cacttggtga gatcaatgga acacctgctt
    57121 ttgtagcgtt cgctaccgca gctgcagtcg caccagatgt cgcaggtcct acgactgctg
    57181 atactttaga ttgggttaca aggttagttg taactgaagc agcctcagct gtttcagact
    57241 tattatcttt atcgattact tcgatttgtt ttccatcgat accacctgcg gcattgattt
    57301 catcaacagc caattgggca cctttttgtt cagctgttcc gtatgcagct aaagaacctg
    57361 attcttcaaa gttaaacccg attttaattg tcttttcctc tactgagtta ccagcagtgt
    57421 tgactgctcc agacttcact tctccacagg ctgcaagaag tgctacactt gcaagcgcca
    57481 caaacgatag ggcaaatttt ttcttcatct tttttgtctc cttatttctt aaatagtctg
    57541 ttaagaatat accgaattat cagaaaattg tcaatgtttt ccttgaactt tttcgatgat
    57601 aacgttttcc tatcgataaa gattgcccac aaagggtgtt tccaactctt ggatatgaca
    57661 aaccctaact tttttaataa atttttcctt gctcaagcgt ccgaccaatt gctccacttc
    57721 ttgagttgga acatagagtt gtaagtaacg atgtttcttg gaatgatagg taatatctcc
    57781 ataatcctgc agttttttgg catcacgatt atagtaaaga tagataatca agccagagcg
    57841 attgactttt tcaaacatga taaatcctct ttctaagaaa tctctcccta ttataccaaa
    57901 aaaagcaaac ccagtcgagt ttgccttcta tcatcattag ttcaatgaat tgttggccat
    57961 aatttcatca ataaagccat attcaagtgt ttcctgggcg ctcatccagt tatcacgttc
    58021 tgcatctgca tggacttttt ccattgactg acctgaattt tcagccaaga ttttttccaa
    58081 ggtattacga gttttgagca agtgttctgc agcgatagcc atatcagttt gttgggtacc
    58141 accacctgta ccgcccattg gttggtgaat catgtattca gcatttggaa gcatgaaacg
    58201 tttgcctttt gctccacttg atgcgatgac tgttcccata gatgcagcca ttcccataac
    58261 aatggtttgg acatctgcct tgataaagtt catggtatct acgattgcca aaccagctga
    58321 aacagaacca cctggtgtat tgacataaag gtaaatatct tttgtactat cttgggcatc
    58381 caagaaaagc aattgggcaa taacagagtt agccatattg tcttcaaccg gacctgtcag
    58441 cataatgatg cggtctttga gaagacgtga gtaaatatcg taagaacgtt ctccacggct
    58501 tgtttgttca ataactacag gaatcattca tttctccttt tgagttttaa ttttgttggt
    58561 caaatgactg aagataagac tattataata tcttggtcaa aaaaggtcaa atttttgctc
    58621 tgctttcatt agacagaaac aaaaacccaa cctcctttcg tgactggaaa tacttttcca
    58681 agtcattctt cttttcgatc ttattttgta ccgaacaagc ggtctccagc atctccaaga
    58741 cctggaacga tataaccgtg ttcgttcaaa cgttcatcca aggctgctgt aaagatttct
    58801 acatctggat gagcttcttg aagggctttt acaccctctg gagcagatac aaggcagaca
    58861 aatttgatat ttgatgcgcc acgtttttta agagaatcaa cagccaagat tgctgagcca
    58921 cctgttgcca acattgggtc tactacaaaa atttgacgtt ggtcaatgtc ctcaggtaat
    58981 ttcaccaagt attcaactgg ttgaagtgtt tcttcatcac ggtacatacc gatgtggcca
    59041 actttagcag ctggaaccaa gttcaagaga ccatcaacca tcccgatacc tgcacgcaag
    59101 attgggacga tggccaattt cttacctgcc aattgttttt gaactgtttt tgtaattggt
    59161 gtttcgattt ccacatcttc tagtggaaga tcacgaagta cttcataccc catcaacatt
    59221 gcaatctcat ctactagctc acgaaaagct tttgtagaag tatctgtacg acgcaagatt
    59281 gacaatttgt gttgaatcag tgggtgatta ataacttcaa tttttcccat ttttggaatt
    59341 ccttctttca atttattctt cttattatac caaaaaacgg tttaaaaatc tttctaaacc
    59401 atttattttt gataattttt acattagatc agcctcttta agagctgtct gtactgtctc
    59461 aagtggtaaa tgggtcaatt ctgtcccttt ttcttgataa aggtattggg cgtagtcgtc
    59521 cattcggtac tggttgatat aaaccacacg cttgcagccg acctgaagca attgttttgt
    59581 acagttgaga caaggaaaat gggttacata ggctgtaaag cctttgggaa caccacgctc
    59641 agcaccttga aggatagcat tgacctcagc gtgaagggtg cgaacacagt ggccttcaat
    59701 gaccaaacat tcgtgatcaa tacaatgctc agtccctgac accgaaccat tgtaaccagt
    59761 ggaaataacc ttattatctt ttaccagaat cgcgcccact ttagcacgtt tacaagtgga
    59821 acgattcgca attagtaggg cttgggctgc aaaatactca tcccaggcca gtcttttttc
    59881 agtcatctct tttctccttt ttctctattt tttaaaaaat ggtaaaccta aatctgcaat
    59941 cttttcagct ggtaccttca tgccatcctt gatccatttt agaaggacag agacgatggc
    60001 tgagctccag aaggaatgaa gataagagct gacacctttt gatttcccat ggtatttttc
    60061 tagaaattcc tgcatggctt ggacaaagat tttttccaga tggtaatcca aggccaattg
    60121 aattactcta gcttcctttc tggcctcccg gaaaaggtga acccaaacca aataaaggtc
    60181 tgtctttaaa tcgtaatgat gcagctgttc cataatattg tggacagttc gtttaaagac
    60241 gctctctaaa atttcctctt tggagtcata attgcgataa aaggccgcac gcgaaacacc
    60301 tgcacgtttg accaattcag aaatactaat cttggtcagt tccttttttt ccaagagttg
    60361 caagagggct gtttcaatgg cttctctggt taataaattg gattcttggt ttgattttct
    60421 gagattttca agagactttt cagagattct acgttcagac ataacatttt ctttctactt
    60481 gtcacaacag acggatgatg cttttgtttc aagggttttc atgatataat tgtaaaaaaa
    60541 gacagaacct ttgtcaatgt ataactgaca aagatggaga atttctatga cttggaagat
    60601 tattgctgac tctggttgtg attatcgtca actgccaaca ccagctatta acacaacctt
    60661 tgtaagtgtc cccttaacca ttcaagtagc tgatcaggtc tttgttgatg acgccagtct
    60721 cgatattgac caaatgatgg aaaccatgta tgcaactgca gaagcttcaa aatcggcttg
    60781 tccaagccca gatgactatt tgcgagcatt tgaaggtgcc aaaaacattt tcctagtaac
    60841 catcacaggt accctttctg gtagtcacaa tagtgctcaa ctagcaaaga atatttatct
    60901 ggaagaccat cctgacacta agattcatgt gattgatagt ttatctgctg gtggtgaagt
    60961 tgacctactc gtagaaaaat tgaatgactt gattgaccag ggcttgtctt ttgaagaagt
    61021 ggttgaagct atcaccgcct atcaagaaaa aactaagttg ctctttgtcc tagccaaagt
    61081 cgataacttg gtgaagaacg gccgtttgag caagcttatc ggtacggtcg ttggccttct
    61141 caacattcgt atggtcggaa aagctagtga aactggaact ctcgaattgc tacaaaaagc
    61201 aaggggatca aagaaatcag ttcaagctgc ctatgatgag ttagtaaaag ctggatatgc
    61261 tggtggccgt attgtcatgg cccaacgcaa taacgaaaaa tgttgtcaac agctctcaga
    61321 gcgaatccgt gaaaccttcc cacaagcgga tattaaaatt ctaccaacct ctggtctctg
    61381 cagtttctat gcagaagagg gcggtttgct gatgggctat gaaattgatt aattgcattc
    61441 ttgacaagag gtgaccatca tctcttgttt ttttgtggta caatagagct atgaaacatt
    61501 ttgatactat tgtcatcggt gggggacctg ctggtatgat ggctacgatt tccagtagct
    61561 tttatggaca gaaaaccctc ctcatcgaaa aaaatcggaa acttggaaaa aaattagctg
    61621 ggactggtgg gggacgttgc aatgtgacca acaatggtag cttagacaac ctgctagctg
    61681 gaattcctgg aaacggacgc tttctttaca gtgttttctc ccagttcgat aatcatgaca
    61741 tcatcaactt ttttacagaa aatggtgtta aacttaaggt cgaagaccac ggacgcgtct
    61801 ttccagccag tgacaagtct cggactatta tcgaagcttt ggaaaagaaa atcactgaac
    61861 taggtggtca agttgctact caaatagaaa tcgtttctgt taaaaaagta gatgaccagt
    61921 ttgtccttaa gtcagcggat caaaccttca cttgtgagaa actcattgtc acaacaggtg
    61981 gtaagtctta tccttcgact ggttcgactg gttttggtca cgagattgct cgccatttta
    62041 agcataccat caccgagctt gaggctgctg aaagtccttt attaacagat tttccacata
    62101 aagccttaca agggatttct ttggacgatg tgaccctaag ttatggtaag catgtcatca
    62161 ctcatgattt actctttacc cactttggtt tgtcaggtcc tgctgcccta cgcatgtcta
    62221 gctttgtcaa aggtggggag gttctctcac tcgatgtttt gcctcaactt tctgagaagg
    62281 acttggttac atttctagaa gaaaatcggg aaaaatcctt gaaaaacgct ttaaaaacct
    62341 tgttaccaga acgcttggcc gaattttttg tacaaggata tcctgaaaaa gtcaaacagc
    62401 tgactgaaaa ggaacgagaa caacttgtcc agtctattaa agaacttaaa attcctgtaa
    62461 ctggaaaaat gtcccttgca aagtcctttg ttaccaaggg tggagtcagt ctcaaggaaa
    62521 tcaatcctaa aacccttgaa agtaagctgg tacctggcct ccactttgca ggcgaagtta
    62581 tggatatcaa tgcccacacg ggtggcttta acatcacttc tgccctctgt accggctggg
    62641 tggcgggaag tctgcattat gattaaagat taaaggttgt gaagaatgaa cagaagagag
    62701 gcagtcgaat ttgttaacat gtgtatgatt aaaaacggag ataaggtcct ggtccaagac
    62761 cgagttagtc ccgactggtc tggcattact tttcctggtg gtcatgttga acgtggcgaa
    62821 tcctttgtcg atgctgtcat tcgtgaagtg aaagaagaaa ctggtctgat catttccaaa
    62881 ccccaactct gtggtatcaa aaactggtat gacgacaagg attatcgtta tgtcgtcctt
    62941 ttttacaaga cagaacactt tactggtgaa ctccagtctt cagacgaagg gaaagtttgg
    63001 tgggaggact ttgaaaatct ttctcatcta aaacttgcaa ccgatgatat gtctgatatg
    63061 cttcgtgtgt ttctagaaga agatctcagt gaattctttt actacaaaaa cggtgacgac
    63121 tggctttatg atttgaagta aaaaataggc taggaaattc ctagcctatt ttttctgcat
    63181 ttgtctgaac gtaagccgca atggcatctg acgtgtactg ggctgttcca gaaccaaact
    63241 tgtcaatgtt ttccttaaac tctgggttgt agacgtaacc tttaccgata tgaccgaata
    63301 cctcaataga gcagtcaaat ccataagtgc gaatggcttg caagagcttg gctgcttgct
    63361 cttggttttc ggttgctgtt gcaggtaaac cagcttgaag attttgtgcc aaagtttgaa
    63421 agacttggtt aaaggcggcc gtagcctcgt tttcgtgacc tttttggcgt tcaagcgctt
    63481 gtcccatgac ttcttgacca tatttctcta ccgcttcttg gtggtatttt tgattgtctt
    63541 gatagctaaa tcccgtgaat ttttcctcaa tggtcatttt tctttctcct ttttgttctt
    63601 gaatagtttt ttgcaaggtg gaaatcaagg tatccagatg ttgcctttcg cgagttagat
    63661 agtccaactg cctagttaaa tggggcaata aatctgtcct ttcttccttt aacagctctg
    63721 ctattttctc taaagaaaag cctagatatt tgtagtaaag aatgacctga aggcgttcca
    63781 aatcctcttg actataggtt cgatagccgt tttccgactt taaggggacc aagagtccta
    63841 tcttgtcata gtgatgcagg gcagggtctt gacagagaca cctgaaagct gcgcagcttc
    63901 ttttatatgg tacatgattt cctccttaca atgatagtat aacccctccc cttaggtcag
    63961 agtcaagcgt ttttgagaaa ggtgtgaaaa cttgaaaatg gtttgcttga cagaccttag
    64021 aaaacataat aggatagtca agtctatcaa tcaaacagaa ggctatttga caactcaagt
    64081 agctttttct tattttgaaa aaggagatca gagtttaact atgtcagaaa aatcacaatg
    64141 ggggtcgaaa cttggtttta ttctagcatc tgctggctgg ccatcgggct tggttccgtt
    64201 tggaagtttc cctacatgac tgctgctaat ggcgatggag gctttttact aatctttctc
    64261 atttccacta ttttaatcgg tttccctctc ctgctggctg agtttgccct tggccgtagt
    64321 gctggcgttt ccgctatcaa aacctttgga aaactgggca agaataacaa gtacaacttt
    64381 atcggttgga ttggcgcctt tgccctcttt atcctcttat ctttttacag tgttatcgga
    64441 ggatggattc tagtctatct aggtattgag tttgggaaat tgttccaact tggtggaacg
    64501 ggtgattatg ctcagttatt tacttcaatc atttcaaatc cagccattgc cctaggagct
    64561 caagcgtcct ttatcctatt gaatatcttc attgtatcac gtggggttca aaaagggatt
    64621 gaaagagctt cgaaagtcat gatgcccctg ctctttatca tctttgttgt tatcatcggt
    64681 cactctctca gcttgtcaaa tgccatggaa ggggttcttt acttcctcaa accagacttt
    64741 tcaaaactga ctagcgctga tctcctctat gctctgggac aatctttctt tgccctctca
    64801 ctaggggtta cagtcatgtt gacctatgct tcttacttgg acaagaaaac caatctagtc
    64861 cagtcaggaa tctccatcgt agccatgaat atctcgatat ccatcatggc aggtctagcc
    64921 attttcccag ccatgtcagc cttcaatatc cagtctgaag ggggacccag cctgctcttt
    64981 atcgtcttgc ctcaactctt tgacaagatg ccttttggaa ccattttcta cgtcctcttc
    65041 ctctttgcga ctgtcacttc ttctgtcgtg atgctggaga tcaatgtggg caatgtcacc
    65101 aaccaggata acagcaaacg tgccaaatgg agtgttattt taggaatttt gacctttgtc
    65161 tttggcattc cttcagccct atcttacggt gtcatggcgg atgttcacat ttttggtaag
    65221 accttctttg acgctatgga cttcttggtt tccaatctcc tcatgccatt tggagctctc
    65281 tacctttcac tttttacagg ctatatcttt aaaaaggctc ttgcaatgga ggaactccat
    65341 ctcgatgaaa gagcatggaa acaaggactg ttccaagtct ggctcttcct tcttcgtttc
    65401 ttcgtttcgt cattccaatc atcatcattg tggtcttcat tgcccaattt atgtaatcaa
    65461 aaagaacttg agtagtgaac tcaggccctt tctttttatg gatggctaac aatcaattcc
    65521 aaaccttgcc cttccagagt ccaagcttca acatcacttg gtaggataaa gtggctgcct
    65581 ttttgaattg gataattttt cccgtcaaca gttagctgac cttgaccagc taagacactc
    65641 aataaactgt agtcagctgt cttttcaaag tcaacttttc cagtaatttc ccacttgtaa
    65701 actgcgaaga aatcattaga tacaaggaga gtggaacgca aatcatctgc tttaacagtt
    65761 acaggacggc tatttgctgg ctcaccaatg ttcaagacat cgatggattt ttcaagatga
    65821 agttcacgca agttgccttt gtcatccttg cggtcaaagt catagacgcg ataggtggta
    65881 tcgctagact gctgggtttc aaggattaag atacccgccc cgatagcgtg catagtcccg
    65941 cttggtacat agaagaaatc tccagcctta acagggactt tggtcaacaa gtcatcccag
    66001 ttcttgtcct cgatttgctg gcggagttct tcttttgact tggcattgtg accgtagata
    66061 atctctgaac cttcatccgc tgcgataatg taccagcatt ctgtttttcc gagttcgcct
    66121 tcatgctcga gtccataagc atcgtctggg tgaacttgga cactgagcca gtcgttggca
    66181 tcgaggatct tggtcaaaag tggaaataca ggttctggac gattgccaaa taattcacgg
    66241 tgttccgcat acaaagtagc aagatctgtt ccctcgtaac gaccattggc aactttagag
    66301 actccatttg gatgggctga gatggcccaa tattctccga ttttttcact tgggatgtcg
    66361 tagccaaact catcacgtag cttggctcca ccccagattt tttcttgcat aactgattgt
    66421 aaaaataatg gttctgacat gtcgatctcc tgtctgattt ttctcccctc attatagcaa
    66481 aaaaagagtt cgaattgaac tcttttttac atcttataaa gcagggagaa gattttataa
    66541 aaatagtaaa caaatgtgct ctacccgatg cttgcaccat tgctatcaat gacatccttg
    66601 taccaataga aggacttctt cttgctacgt ttgagagctc cgtttcctac attatctcga
    66661 tctacataga taaagccata gcgcttattc atttcccctg tgccagctga aactggatcg
    66721 atacagcccc aagtcgtata accaagcaag tcaaccccgt cttggtaaat ggcatctcgc
    66781 atggccttga tgtgggcctc taagtaagta atccgatagt catctgctac ataaccattc
    66841 tcatccggtg tatccatagc acctagtcca ttttctacga taatactaaa ctaaaatcaa
    66901 aaagcattat ataatagtga tatgaaatca actaaagaag aaatccaagc ca
//
LOCUS       DAVKAO010000005        54576 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKAO010000005 DAVKAO010000000
VERSION     DAVKAO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.005"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(381..1577)
                     /gene="tuf"
                     /locus_tag="VWX16_000324"
     CDS             complement(381..1577)
                     /gene="tuf"
                     /locus_tag="VWX16_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9157739.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2488..3357)
                     /locus_tag="VWX16_000325"
     CDS             complement(2488..3357)
                     /locus_tag="VWX16_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9157740.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYTATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3580..4260)
                     /locus_tag="VWX16_000326"
     CDS             complement(3580..4260)
                     /locus_tag="VWX16_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9157741.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDK
                     PTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGF
                     FKKKEDEK"
     gene            complement(4218..5324)
                     /locus_tag="VWX16_000327"
     CDS             complement(4218..5324)
                     /locus_tag="VWX16_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9157742.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVDSGDSKLGKGGYFSTGDVLLGKDVVSYTVRVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFEEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5435..5689)
                     /locus_tag="VWX16_000328"
     CDS             complement(5435..5689)
                     /locus_tag="VWX16_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157743.1"
                     /translation="MLNVDQDFMSISKSNKSDSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(6029..6562)
                     /locus_tag="VWX16_000329"
     CDS             complement(6029..6562)
                     /locus_tag="VWX16_000329"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU9157744.1"
                     /translation="MTSFKEQESEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVGGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLERFFKTR"
     gene            complement(6549..6764)
                     /locus_tag="VWX16_000330"
     CDS             complement(6549..6764)
                     /locus_tag="VWX16_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9157745.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(6731..6874)
                     /locus_tag="VWX16_000331"
     CDS             complement(6731..6874)
                     /locus_tag="VWX16_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU9157746.1"
                     /translation="MAYSTDFKQRALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            7108..8826
                     /locus_tag="VWX16_000332"
     CDS             7108..8826
                     /locus_tag="VWX16_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9157747.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8937..9284
                     /locus_tag="VWX16_000333"
     CDS             8937..9284
                     /locus_tag="VWX16_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9157748.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9562..10398)
                     /locus_tag="VWX16_000334"
     CDS             complement(9562..10398)
                     /locus_tag="VWX16_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9157749.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKLSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10411..11040)
                     /locus_tag="VWX16_000335"
     CDS             complement(10411..11040)
                     /locus_tag="VWX16_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9157750.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAESYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALNPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11050..11691)
                     /locus_tag="VWX16_000336"
     CDS             complement(11050..11691)
                     /locus_tag="VWX16_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9157751.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11837..13066)
                     /locus_tag="VWX16_000337"
     CDS             complement(11837..13066)
                     /locus_tag="VWX16_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9157752.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(13056..14222)
                     /locus_tag="VWX16_000338"
     CDS             complement(13056..14222)
                     /locus_tag="VWX16_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9157753.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14366..15379
                     /locus_tag="VWX16_000339"
     CDS             14366..15379
                     /locus_tag="VWX16_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9157754.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15424..15843)
                     /locus_tag="VWX16_000340"
     CDS             complement(15424..15843)
                     /locus_tag="VWX16_000340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9157755.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15854..17260)
                     /gene="atpD"
                     /locus_tag="VWX16_000341"
     CDS             complement(15854..17260)
                     /gene="atpD"
                     /locus_tag="VWX16_000341"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9157756.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17346..18224)
                     /locus_tag="VWX16_000342"
     CDS             complement(17346..18224)
                     /locus_tag="VWX16_000342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9157757.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLSDQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(18240..19745)
                     /locus_tag="VWX16_000343"
     CDS             complement(18240..19745)
                     /locus_tag="VWX16_000343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9157758.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19760..20296)
                     /locus_tag="VWX16_000344"
     CDS             complement(19760..20296)
                     /locus_tag="VWX16_000344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9157759.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20296..20790)
                     /gene="atpF"
                     /locus_tag="VWX16_000345"
     CDS             complement(20296..20790)
                     /gene="atpF"
                     /locus_tag="VWX16_000345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9157760.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20804..21520)
                     /gene="atpB"
                     /locus_tag="VWX16_000346"
     CDS             complement(20804..21520)
                     /gene="atpB"
                     /locus_tag="VWX16_000346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9157761.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21555..21755)
                     /locus_tag="VWX16_000347"
     CDS             complement(21555..21755)
                     /locus_tag="VWX16_000347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9157762.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21830..22837)
                     /locus_tag="VWX16_000348"
                     /pseudo
     CDS             complement(21830..22837)
                     /locus_tag="VWX16_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(23282..23680)
                     /locus_tag="VWX16_000349"
     CDS             complement(23282..23680)
                     /locus_tag="VWX16_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9157763.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(24008..24490)
                     /gene="greA"
                     /locus_tag="VWX16_000350"
     CDS             complement(24008..24490)
                     /gene="greA"
                     /locus_tag="VWX16_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9157764.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(24561..26216)
                     /gene="mltG"
                     /locus_tag="VWX16_000351"
     CDS             complement(24561..26216)
                     /gene="mltG"
                     /locus_tag="VWX16_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9157765.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLIKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(26297..26791)
                     /locus_tag="VWX16_000352"
     CDS             complement(26297..26791)
                     /locus_tag="VWX16_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9157766.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(26788..27252)
                     /locus_tag="VWX16_000353"
     CDS             complement(26788..27252)
                     /locus_tag="VWX16_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9157767.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEETLLYVTSQGYSMIWFNPFYLEEQ"
     gene            complement(27262..28596)
                     /locus_tag="VWX16_000354"
     CDS             complement(27262..28596)
                     /locus_tag="VWX16_000354"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9157768.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(28607..29224)
                     /locus_tag="VWX16_000355"
     CDS             complement(28607..29224)
                     /locus_tag="VWX16_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9157769.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(29272..32376)
                     /locus_tag="VWX16_000356"
     CDS             complement(29272..32376)
                     /locus_tag="VWX16_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9157770.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINRQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADTNLFAFTVTEYLDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKVLPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDVDLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQKLGQFELKDGTLQARKSLAYSLAHLFEGRDRDRVSFSQEFQNLAQ
                     DLTHPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFL
                     TSQVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADSHQIYVT
                     SYATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENH
                     LGELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVY
                     KNELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMED
                     YQGASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPA
                     KERQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHR
                     MGQEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGIS
                     EAST"
     gene            complement(32469..33635)
                     /locus_tag="VWX16_000357"
     CDS             complement(32469..33635)
                     /locus_tag="VWX16_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9157771.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHSRINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(33643..34737)
                     /locus_tag="VWX16_000358"
     CDS             complement(33643..34737)
                     /locus_tag="VWX16_000358"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9157772.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDRADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(34894..36576)
                     /locus_tag="VWX16_000359"
                     /pseudo
     CDS             complement(34894..36576)
                     /locus_tag="VWX16_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(36748..38706)
                     /locus_tag="VWX16_000360"
     CDS             complement(36748..38706)
                     /locus_tag="VWX16_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9157773.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAVDKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(38909..40531)
                     /locus_tag="VWX16_000361"
     CDS             complement(38909..40531)
                     /locus_tag="VWX16_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9157774.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTTKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            40637..42082
                     /locus_tag="VWX16_000362"
     CDS             40637..42082
                     /locus_tag="VWX16_000362"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9157775.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLCLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(42128..42322)
                     /locus_tag="VWX16_000363"
     CDS             complement(42128..42322)
                     /locus_tag="VWX16_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9157776.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(42423..43031)
                     /locus_tag="VWX16_000364"
     CDS             complement(42423..43031)
                     /locus_tag="VWX16_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9157777.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDHL
                     MQKCRDIFVDSLVVLGYIVQNEDRKYELAIDFDKERLTFYLA"
     gene            complement(43056..43568)
                     /locus_tag="VWX16_000365"
     CDS             complement(43056..43568)
                     /locus_tag="VWX16_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9157778.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(43654..44589)
                     /locus_tag="VWX16_000366"
     CDS             complement(43654..44589)
                     /locus_tag="VWX16_000366"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9157779.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(44660..44959)
                     /locus_tag="VWX16_000367"
     CDS             complement(44660..44959)
                     /locus_tag="VWX16_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9157780.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(44949..45698)
                     /locus_tag="VWX16_000368"
     CDS             complement(44949..45698)
                     /locus_tag="VWX16_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9157781.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(45752..46111)
                     /locus_tag="VWX16_000369"
     CDS             complement(45752..46111)
                     /locus_tag="VWX16_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9157782.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(46113..47513)
                     /locus_tag="VWX16_000370"
     CDS             complement(46113..47513)
                     /locus_tag="VWX16_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9157783.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(47808..48047)
                     /locus_tag="VWX16_000371"
     CDS             complement(47808..48047)
                     /locus_tag="VWX16_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9157784.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(48079..48549)
                     /gene="ssbA"
                     /locus_tag="VWX16_000372"
     CDS             complement(48079..48549)
                     /gene="ssbA"
                     /locus_tag="VWX16_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9157785.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(48561..48851)
                     /locus_tag="VWX16_000373"
     CDS             complement(48561..48851)
                     /locus_tag="VWX16_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9157786.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(49004..50350)
                     /gene="asnS"
                     /locus_tag="VWX16_000374"
     CDS             complement(49004..50350)
                     /gene="asnS"
                     /locus_tag="VWX16_000374"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9157787.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     DMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(50366..50731)
                     /locus_tag="VWX16_000375"
     CDS             complement(50366..50731)
                     /locus_tag="VWX16_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9157788.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLTFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(50724..51911)
                     /locus_tag="VWX16_000376"
     CDS             complement(50724..51911)
                     /locus_tag="VWX16_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9157789.1"
                     /translation="MKLSNRVLEMEESVTLATGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(51908..52396)
                     /locus_tag="VWX16_000377"
     CDS             complement(51908..52396)
                     /locus_tag="VWX16_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9157790.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            52717..53265
                     /locus_tag="VWX16_000378"
     CDS             52717..53265
                     /locus_tag="VWX16_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9157791.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            complement(53543..54271)
                     /gene="yaaA"
                     /locus_tag="VWX16_000379"
     CDS             complement(53543..54271)
                     /gene="yaaA"
                     /locus_tag="VWX16_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9157792.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(54350..54514)
                     /locus_tag="VWX16_000380"
     CDS             complement(54350..54514)
                     /locus_tag="VWX16_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9157793.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
BASE COUNT        15939 a        12004 c        10063 g        16570 t
ORIGIN      
        1 actattgtat aatgcttttt gattttagtc tagtattata ctcttcgaaa atctcttcaa
       61 accgcgtcag cttcgccttg tcgtatagat gttactgact tcgtcagttc tatctacaac
      121 ctaaaaacag tgttttgagc agtctgtagc taactttcta tagtaaaatg aaataagaac
      181 aagacaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa gtaaatgtgt
      241 actattctag tttcaatata ttatagtttg ctctttgatt ttcattgagt attagttcaa
      301 tatctaaaaa aagaaccttg ccaagcaaga ttcttttagt gtctgactta aataattgtt
      361 cttctgggaa ctaaatcgaa ttaagcttcg atttctgtaa ccatacctga accaacagta
      421 cgtccaccct cacggataga gaatgtagta ccttgttcta cggcgattgg gtgaatcaac
      481 tcaacgtcga ttgtcacgtt atcaccaggc attaccattt cagtacctgc tggaagttcg
      541 attgaacctg taacgtcagt agtacggaag tagaattgtg gacggtagtt gttgaagaat
      601 ggagtgtgac gtccaccttc ttctttagta aggatgtaga cttcaccttt gaatttagtg
      661 tgtgggttga ttgaacctgg tttagcgata acttgtccac gttcgatttc atcacgttga
      721 acaccacgaa gaaggacacc tacgttatct ccagcaagac cttcgtcaag ttgtttacgg
      781 aacatttcaa caccagtaac aactgctttt tgagtttctt ctttgatacc aacgatttcg
      841 atttcgtcgt tgactttaac gataccacgg tcgatacgtc ctgaagcaac tgtaccacgt
      901 ccagtgattg agaatacgtc ctcgactgga agaagcaatg gtttgtcagt gtcacgttct
      961 ggttctggga tatactcatc aactgtgttc atcaattcca taacgatgtc ttcgtattta
     1021 gagtcacctt caagagcttt aagtgctgaa ccttggataa ctggaagatc gtcacctggg
     1081 aagtcgtatt ctgacaatag gtcacggatt tccatttcaa ccaattcaag caattcttcg
     1141 tcgtcaacca agtcaacttt gttcatgaag acgataaggt gtttaacacc aacctgacgt
     1201 gaaagaagga tgtgctcacg agtttgtggc attggtccgt cagttgaagc tactacaagg
     1261 atagctccgt ccatttgagc agcaccagtg atcatgtttt taacgtagtc cgcgtgtcct
     1321 ggagcgtcga tgtgagcgta gtgacgtttt tcagtttcgt actcaacgtg cgcagtgttg
     1381 atagtgatac cgcgttcgcg ttcttctgga gcagcatcga tagacgcata gtctttaggt
     1441 tggttaactg atgaaggcaa gcgacgtgcc aaaacagttg tgatagctgc agttagggta
     1501 gttttcccgt ggtcaacgtg tccgatagta ccaatgttaa cgtgtggttt actacgatcg
     1561 tatttttctt ttgccatttg agtaaaagcc tccaataaaa tatattttat agatagacag
     1621 taggcaatac agtctaactt tccttactat tttatcaaat ttcagctaaa ttgcaagtgt
     1681 tttacaacgt ttttgtgaat tattcttgat aaaaatttaa ctaactaata gaaagtacta
     1741 aatagactta tccttcaagg agttttcagc attatccatc cactcacttg ctgaaaatgt
     1801 aaacagtctc tggtttatat agtaaaatga aacaagaata gggcaaatcg atcaggacag
     1861 tcaaatcgat ttctaacaat gttttagaag ttgaggtgta ctattctagt ttcaatctac
     1921 tatacaacct caaaactgtg ttttgaacaa cctgcggcta gcttcttagt ttgccctttg
     1981 attttcattg agtataaaat tgcagataaa acgataacaa tcataggact cagcagagtc
     2041 aatgtaggtc gaagaatagc cctcctaatc gcttatttta tagcaaaatg aaatagaaac
     2101 tgaacatttt gttgaggaag gtaaatcaat tcctcacaat gtttgagaaa gaagggagga
     2161 ctattccaac ttcaacccac tataaagcag acttacaaga tagggaaccc atctccgata
     2221 atatatagta gaatgaaaac tgcacaattt gattggagaa ttcaaaccaa tttctaacaa
     2281 tgttttagaa acaagggtct attatttcaa ctgcaaccta ctatagccca ttgaagctgg
     2341 aatagcccac tgagacttct aaaccactgc tggagaggag tttgtttaga gtttctttca
     2401 tttccctaaa aaatcaaaaa gagagaacca gtctgatcct ctcttactta tagatggtgc
     2461 tcactcaaat gttaaaggag ctatttctta gagataaagg aatttgaata cagctactgc
     2521 cgcaattgct gctgcgatag gtgctactac tggtacccaa gaataccacc attttgaatc
     2581 gcctttatgc tcaccaagaa ctgattttgg aaggaaagca tgaaggagac gtggtcccaa
     2641 gtcacgggct gggttcaagg caggtcctgt aggtcctcca agtgatgtta ccaaagccat
     2701 aacgaggaat ccaagtgcca agtgagccac agaaagtcct gaagcagtgt gtggagccac
     2761 ctgtgcttta atagccaagt cagaaaaatc aactgtttgt cctgcttcta ttgccttttg
     2821 tttcatgaat tgaagcactt cagcaccaaa gaagttttta gtcaaaccaa gagctgcaaa
     2881 gaacaaaaca aatgaaccaa caaactcatt aatcaaacca ttgacagttg ctgtatagcg
     2941 actttctttt gtaccatggt caatacttga aatagttgag aaagttccca agatgttatt
     3001 tggtttttca gttttcaagt agaatggacg gtatgttgcc acaactaagg cttggccaaa
     3061 gatagccccc aagacttgcg cgataatgta aggtaccact tgtgcccaag ggaaaagacc
     3121 gctaactgca agccctagag tgaaagcagg gttgatgtga ttcccagata cgttaccaaa
     3181 catcaaggct gggatcataa cccccatacc ataaccaaca gcgatgacga tccagccact
     3241 ttggtgacct ttcgtacctt taagttcaac gttggcaact gcaccattcc caagaatgat
     3301 caaaatggca gttcccaaaa attcagtggc atacttcagt gcccatgtga aatccatttg
     3361 ctagattctc cttaaaattt tttagatatt ccttatttta tcaattttta ccctatttag
     3421 caactgattt ctggaaatat ttttaaaaaa ataggattca tagtttccca gttcaacgta
     3481 cttgtaagcg tttttattct tcacgaaaga cctcattctc aaaaccagga aaaagcccac
     3541 atctgtgatc ttttccttgt tcctagattc taaaatctat tatttttcat cttctttttt
     3601 cttgaagaat cctaatccta gaccagctaa caaggtcatg ataccagcac tagccaaggt
     3661 agcattagct tctgtacctg tgtttggcaa ttcctctctc ttacctgtct cataagtcgg
     3721 aacttctggg tctggattca ctggagtttc agtttttgga gtacctggtt ctggagttgg
     3781 tttatctggt gttggagtct cttgatttgg tagttctggg tttggtgtcg gaacacttgg
     3841 atgctctgga tttggttgtg ggattggttt ttctggagtc ggttcacctg gagtacctgg
     3901 ttcctgagga cttggaattt ctggtttttc tggagttggt ttatctggtg ttgataaacg
     3961 gtcatacctt accgttattt ctttatcact agagtctgac gtaacttctt gtgattcaac
     4021 tgttggaata tctggatctt tgtacttgtc aatcttacca gatataacct cgtcccagtt
     4081 tcctgttgtc cattcaccgt aggttacaac tcccgtgacc ttgttctcag tttttgtacg
     4141 gcttaaggtt acaggttgaa caacatcttc ttttacattt tggttcgtaa ctttatcaac
     4201 gtaatgaatg atacgcgtta tagtcttcgt ctcagtagag gttgctgttt tgggaaccac
     4261 tgtttcctca acattctcac ggtagtaata gtcaactgtt gcaccgtctt ctggtacgca
     4321 tttgcaggag ttgctaccaa ggtgtatgtt gttttccttg tgataactcg gtcttctttg
     4381 tcctcagttg ttgttttccc ttcaatagtt tttgattctg tggtatactc agaacctatc
     4441 gctaaatcag cttttataac agactctgcc aacttctctt ggctaccttc tttatagtaa
     4501 ttggctgtta ctgtagcagt ggttggcgct tcgctttctc tataaactaa ggtcactgtt
     4561 ctaccttcgc ttacaatatt cccagttaaa cttgcagaat ttgtatctgc ttcttcaaaa
     4621 gtataatatt ttccgtcagt agtagtcatg ctactgagtt ttttatctgt gacattatag
     4681 ctggtaccaa tcagttgttt tttattggta atgtaggttc cgtcactttc tttttctcca
     4741 attccagtat catttttcaa tgatagcaaa cgcccttgtt catcaacata gcgaactttc
     4801 acattttctg agattagttc tgccaattct gaggtttttt tctttttctt gatttcttcg
     4861 gttattttcc ctttctcttc ttcgggaata tttagttttg gaatgatttt ttcaacaacg
     4921 gttcgtgatg gttccacagt atcttggatg actgaaaagt cagctagaat tgggagatta
     4981 tactgaacac ggtgactttg agtgtttact cctactcttt cattattctc tgaaaatact
     5041 cgtacggtat aagaaacaac atcttttcct aatagaacat ccccagtaga gaaatagccg
     5101 ccttttccta gtttgctatc tccagagtcc acttctttcc taatcttatc agatagtttt
     5161 ttaccagtca gtacattcgt tcgcacaatc cctttgtcta cccctacaaa gtgggagaac
     5221 tttttgaact cttcagaacc agatctagcc caaccattat taagggcatt tgcttttgta
     5281 tttgtattct ctctcaaagg tttggcgatt agaattatat tcatcggcac ttagagttgc
     5341 tgctatatct gactcttgaa taccaacttc cttactacca tttctagcgg cagtatatgt
     5401 gtatttatct gtttctactt ctagctgatt atccttaaca tgataggtaa ttttctcctg
     5461 gtataagagt cttcagtagt ttatatgctc tgtcatcatc tttagcttta gaagcagctg
     5521 agtttagcca agttggggta ttaggagtat ctttatcagc ctgtttcaag acattattca
     5581 agtcatttgc taacctattg gttgtcctca ctgtgaaagt tttcttccag tctgaatcac
     5641 ttttattaga cttagatatg ctcatgaaat cctggtcaac attcaacaca atatcgcctt
     5701 gttttttagc atcttccgtt gcttctgctt caaattgatc atggagtttt tgttgctctg
     5761 gtgtatagtt tttttattta cacgatttaa tgtactattt tttattttag tattaagaac
     5821 tccattaata cgatttttaa actctgcttc aaaaaatccc ttaatttgtt ggtgcgcaag
     5881 ttcaacattc aaacctttct tatacaagtc ttcattctga acgatttcgg ctacacggtg
     5941 aatggtttga tcgtataccg tcttctctta accagcagta tccttagttt taatgactac
     6001 tcgataggtt cctttagaac cgttatagtc atctagtttt aaaaaagcgt tccaaacatt
     6061 caaaaatagt tggaacttcc cttaataaat ccgtcacttt ctttttcaag atagcccaag
     6121 cttgctcaat aggattcaaa tccggtgaat aaggtggtag aggtaagaaa aagtgtttat
     6181 cttggatgca gagttcatcc aaaatgttct tgggatgaaa actagcattg cccatgacaa
     6241 taacatgagg tgtcttcaaa gcaggtagga gttgcgtttt gaaccactcc acaaagaaat
     6301 cgcttgtcat gcttttcttg taaatcatgg gagctataaa ctctccgcct acttgtcctg
     6361 caacaattga agttcgctca aaacgacgtc cgctaatctt ttcatagact ttctcccctc
     6421 taggagcccc tgcataggga cgatataggt agcggtcgat tcctgtttca tcaatatata
     6481 cgattggtaa atcttttagg ttatccaaaa tatcaagaaa ctcagcgact ttctctgact
     6541 cttgttcctt aaagctcgtc atcttttttt aaagtgacct taatctgctt taaagctgcc
     6601 catactgaag gaacagcaca atcaaaatgt gccgcaattt cccgtaaaaa agcatctgga
     6661 tgagcctcta caaaggcttt caattcctct aaaggaatct ttcggttttt gacgactcgc
     6721 tttttcctct ctatgtgtcc ttgttcacgt ctttcttttc ccacgtgaag agagttctga
     6781 cgccaacacc aaaaaacttg gctgcctcga catgactgtg cccctctttg atggaatcta
     6841 atgctcgctg tttaaaatca gtactatatg ccatagcttt attataacat gtattttata
     6901 aactaagcta ctatatcaat gttttgttat gagaagactt acttttttta ctgaaattga
     6961 atttttcctc gcctcaacat catataaccc caaaattcaa tctgacctgg tttgaagaga
     7021 tgttcgaagc agataaggtt ttactaaaac taaaaaaaat gatataatca aactgattaa
     7081 atgttttaaa aataaaggag aatttgaatg tcttaccaag aaaattacca gaaatgggtt
     7141 gattttgtgg agcttcctga ctatcttcgt caagatttgg aaaatatgga cgaaaaaact
     7201 aaggaagatg ccttctatac aaatcttgaa tttggtactg caggtatgcg tggcttggtt
     7261 ggtgctggta caaaccgtat caacatctac gttgttcgcc aagctactga aggattggct
     7321 cgtttgattg agtcaaaagg tggaaacgag aaagaacgcg gtgtagcaat tgcctacgat
     7381 agccgtcact tctcacctga gtttgccttt gaatctgcgg cagttcttgc taaacacggc
     7441 atcaaatctt acgtatttga aagccttcgt ccgactccag aactatcatt tgcagttcgt
     7501 catctcaact gtttcgcagg tatcatggtc acagccagcc acaaccctgc tccatttaac
     7561 ggttacaagg tttacggtga agacggtgga caaatgcctc cacacgatgc ggacgctttg
     7621 actacttata tccgtgcaat cgaaaaccca tttgcagttg aagttgctga tgtggaaact
     7681 gaaaaagctt ctggcttgat tgaagttatc ggcgaagctg ttgacgtaga ataccttaaa
     7741 gaggttaagg acgtaaacat caacccagcc ttgattgaag aatttggtaa agacatgaag
     7801 attgtctaca caccacttca tggtactggt gaaatgttgg ctcgtcgtgc tcttgcccaa
     7861 gcaggatttg actctgttca agttgttgaa gcgcaagcaa ctgctgaccc agacttctca
     7921 actgtaacat ctccaaaccc agaaagccaa gcagcctttg cccttgctga agaacttggt
     7981 cgtcaagttg gtgcagatgt tcttgtcgca actgacccag acgctgaccg tgttggtgtt
     8041 gaagttcttc aaaaagatgg tagctacctc aacctttcag gtaaccaaat cggtgctatc
     8101 atggctaaat acatcttgga agctcacaaa aacgctggaa ctcttcctga aaatgccgct
     8161 ctctgcaaat ctatcgtttc aactgacttg gtaacgaaga ttgctgaaag ctacggcgca
     8221 actatgttca acgtcttgac aggtttcaaa tttatcgctg aaaaaatcca agaattcgaa
     8281 gaaaaacaca accacactta catgatggga tttgaagaaa gcttcggtta cttgattaaa
     8341 ccatttgtac gtgataaaga tgccatccaa gccgttcttg tcgttgctga acttgctgcc
     8401 tactaccgtt ctcgtggttt gacacttgct gacggtatcg aagaaatcta taaagaatat
     8461 ggctactacg cagaaaaaac aatctctgtt actctttcag gtgtcgatgg tgctgaacaa
     8521 atcaaagcga ttatggctaa attccgcaac aatgctccaa aagaatggaa cgcaacagct
     8581 atcactgtcg tagaagactt caaggcacaa actgctactg ttgctgacgg tactgttaca
     8641 aacttgacaa ctcctccaag tgatgtgttg aaatacacac ttgctgacgg ttcatggatt
     8701 gccgttcgcc cttcaggtac agaaccaaaa atcaagttct acattgcagt tgtaggggaa
     8761 accaacgaag aatcacaagc taagattgct aacatcgaag cagaaatcaa tgcatttgta
     8821 aaataaagac ctataaaaga tgagaccaac caacctcatc ttttttcgta tcaaatctaa
     8881 gcaattttag aaactttatg gcatactaga cttatcaatg aaagcgaggt aatctcatgg
     8941 aacaattttt agataacatc aaagaccttg aagtcactac agttgtgcgt gcgcaagaag
     9001 ctcttgataa aaaagaaact gcaaccttct ttatcggtcg caaaacttgc ccttactgcc
     9061 gtaaatttgc aggtacattg tcaggtgtcg tagctgaaac caaagctcac atttacttca
     9121 tcaatagtga agaaccaagc caactcaatg atttgcaagc attccgctca cgctatggaa
     9181 tcccaactgt accaggtttt gttcatatta cagatggaca aatcaacgtc cgttgcgact
     9241 cttcaatgtc agcacaagaa atcaaagatt tcgcaggatt gtaaaaaatc agctaacaag
     9301 tcttaaaatc acaaaaacgc ataatgtaag gtgcaaaaac cttgatatta tgcgttttat
     9361 tgttcctaaa acagttacat cagcatttca ttacagaaca ttttaatttt ggaagtcaaa
     9421 tcaattttta acaatgtttt agaaatcaca gtgggctatt ccatattcaa ttatccagat
     9481 tcaatcgcta ttatcgtttt cctagattac aaaaaaactg cgttttgcta atcaaaacgc
     9541 agtttcagcc actattttat cttactgtcc ttcttttact tctttggttg ctacatcttc
     9601 tccaaaccat ttttggctga tttcttggaa cttgccgtcc ttgtaaagac tagaaaaagc
     9661 ttcatttatc ttcttaacca agtttgtatc ttccttacgg gctccaaccg caaaagcttc
     9721 tgtttctagt ccaactgtaa agacattata atcgtttaaa acaccttctg cttctaaata
     9781 ataatttgca tagacacggt caatcaatag accatcaatt cgatcgtttt tcaaatcaat
     9841 caaggcttca ttaaaggttt ggtattgatt cgcttcctta ttagcgacaa tattcttcaa
     9901 aagttctgga tttgcttcaa agtccgcata accagatgaa ccagcttgag ctcctaatgt
     9961 ctttccagtc atatcctttg cagtcgtgat accagataat ttcttcgtaa ctaatacctg
    10021 ctcattcttc atatatgagt tactgaaagc caccttttca cggcgttcgt ctgtagcgga
    10081 atagccattc caaatcagat caatcgttcc ttttgtcaat tcagcttctt tcaaatccca
    10141 atcaatcggt tgccaattta ccgtgattcc gtatttttca aaaacagctg tagctaaatc
    10201 aatatcaaat cctgcataag aaccatcttt ctgagcaaat cccattggaa caaaagtact
    10261 atcaaatcca atagtaatag acttgttaga ctggtacttt gaccaattat ctccactagt
    10321 ttcgctagaa tttttcccac aagctactaa gaacaaagca gtcatcagac tgactaatac
    10381 aagcatccat tttttcatcc atttttcctc ctatttaggt tctactttca ataatacatc
    10441 tgcgatattt tcagcaaact gcaaatcatg ggtaaccaca atctgggtca tcccaagttc
    10501 cctattttgc aagattagct tctccacttc caaacgtaat tctggattca gggcagaagt
    10561 tggttcatcg tagccaatga tttctgggtc aatcatcata gcacgcgcca aagccacccg
    10621 ctgcttttgc ccaccagata gtgagaaagg ataggactct gcgtgtcctc ctagtcctaa
    10681 ctgttccaag agtccactcg ccttcttctc agcctcttcc tgcttcattc ccatggtctt
    10741 cacaggcgat aaagtcaaat tttccagaac tgatagatga ggaaatagtt gaaaatcttg
    10801 gaagacaaat cccagtaggt tgcgcttctg caattcatcc agctctaaag gttgtccatt
    10861 ataaaagatt tgccctgaat caatggtttc aagacccgca agcatacgta aaagagtcgt
    10921 cttacctcca ccagaaggtc caacgatagc caggatttgc ttttcaggaa tacttagact
    10981 gaaatcagac aggatttgtt tgtctccaaa gactttattg atatttcgta attctaacat
    11041 agctgccccc tatctataat aactgtactt tttctcaact tttttggaaa taattgtcac
    11101 aatcccaatt aaaatcaagt aaatagctcc tgccaagaac ataggaacca gactagcatc
    11161 gcggttagca gctgttcgac tagccaagat aaggtctgaa attccgagag cgtagaccaa
    11221 agaagtatcc ttgaccaaac tcataacttc attaaagaca ctaggaagaa cgatcttggt
    11281 cacttggggc aagataatat agcgcactct gtcaaaaggg ctaaacttca agaccttggc
    11341 agcctcatac tgtcctcttg gaatagtgtc aattccccca cggaaaattt ctgcaaagta
    11401 agctgcatag ttgagaacaa aggcaataat agctgcagga aggcggtcta aacgaatccc
    11461 aatacttggg agcacataat agataaaaat cagttgcaag agtaaggggg ttcctcgcat
    11521 aacccagatg taaatgttaa tcagataatg gaggggcttc caatggactt gcaaggcaaa
    11581 ggcaatcaaa acgcccaacg gaatcgaaaa tagcaagacc agtgcaaaga cctgtacagt
    11641 cgtgctcgca ccattcagta aactcggtaa tatctcaaat aaataagaca tactgcacct
    11701 cctaaaagta attgttccta ttatagcatg aataaacaag attaccaagt atttttgtaa
    11761 aaagattcta ccatatacaa ataaaaaccg tagctatcaa aagagataaa ctacagttct
    11821 atatgtttaa acattcttac aatctctcaa acagttcttg catttgcaca tcatctggaa
    11881 ccagttttaa gtaagtgtga gcatggactt ttgcttcttc aaaatgtccc aattcacgca
    11941 agagatagat atagtgttcc agaaattctg gattgtcctt caaatctcct gtcaactctt
    12001 gataatgctc ataagcagta tccaaatcgt ccatttcttg ataagaacga gcaatcatcc
    12061 acttggtcaa aagattttct ggctcctcac tctgcaagtc tagaatatcc tcataacgct
    12121 cctgctccag ataaatagtg gctaaacgaa gcaagatttc ttctgtatcc tcagcgtctt
    12181 cttttgcagt aaggagataa ttttctgcac cactagcatc atgcaattca taagaaaatt
    12241 gtgaagcagc tagcaagagg cgagtttcaa agggattttt ctctaatcct tgcttagcga
    12301 tacgcagggc ttcttgaact tgatgttcct tatgtaaagc ctgactgtac ccatactcat
    12361 agccttcaaa gtcaggagaa atggtatcaa gctgcttaaa gtagagggtg gctttttgat
    12421 attcttcttg atcaaaataa agactggcca actcaaaagc tgttaagtca tcgtattcta
    12481 actccagggc tttttctaaa aactcagtag ccgtttcaaa tttccctaac tgagcatagg
    12541 caaagccaat tcgttgatag gtggaaatgc ccgtttgctc ataaatcgag cgattatcta
    12601 actgggcata ggcttgaata gccgcttggt aattttccaa ctcactatcc aactctgcca
    12661 aacccaatat caagagagaa tcctctgagt aggtcaaggc ctccaataat ttctcacgtg
    12721 ccacatctgt caaaccttcc agctggtaaa ggtctgcctt cagagccaaa gacgagacat
    12781 accagtcact gtcagcttgg atttcctcaa gataggtaaa ggcttcttct atttgaccat
    12841 cctcgctagc aattgcagct agattaagat gaacctctgg aaaatcctct acaattttca
    12901 ggtaaatttc cttggcctga ggatagaaac caatcccttc aagataagtt gccaactcat
    12961 acagaagatc acttgaatca ttttctaaag ctttggcgaa ataatgctca gccttagtta
    13021 aatcttgctc ctccaaagcc tgtaacatct gttgactatt gttcactctt gacttcctca
    13081 ccctctaatt catatagacc actgactacc ttataccatt cgaaaatggc tgaaatgaca
    13141 accttagcag aggcataaac cggaatacca agtaaaactc cccagatacc aaacatagat
    13201 cctgaagtta acaaaacaaa gagaacatta atagggtgga tgtttaattg acttcccaaa
    13261 atcaatggag agacaaaacg gccttcaata gtttgttcta caataaagac aatcactact
    13321 ttcaaaagca tgactggacc agcaatcaaa cccaatacta gggcaggaag catggctaga
    13381 aagctaccaa gataagggac cagatttaaa ataccagcag taacccccag cgtaaccgca
    13441 tagcgtagac caataatctt gaagaagatg ataaacatta ctgctacaat aatagccact
    13501 gtcacttgcc ctcgaacata gttggacaac tgttgattca catctgatag aacttgtcca
    13561 acaggttcct tcaattttct tggaatgaat tgggtcaaat agttacgcaa gcctttccca
    13621 tcacgcaaga gataaaagag catgaaagga acgataatca aggcaacaat cacttgagaa
    13681 gctccgctaa taaaggcact cacccagttg actgcctgag atgaaacctt acttgccaaa
    13741 actgtagcct ggctagaaaa attggtcaaa acttgctcta attgaggtct gaaatcatct
    13801 ggcaggtgct gggctaccaa tccattaaca atcctatcta tatcttctaa gtaaacagga
    13861 acgtttcttg caaaggtcaa aacctgacgt tgcagatttg gaatggcgac tgccaagccc
    13921 caaatgataa agagagcgat gataacaaag acaatagtga tagctataac acgattaacc
    13981 ttatgcttct ccatccaatc aacaatagga ttcaacaaat aatataacaa accagacaaa
    14041 atgactggca acatcacaac tgctaaaaag tctaaaacag gtgaaaatag aaaactaatc
    14101 ttacttaaaa taaaaagatt caatcccaat aataaggtta ctaaaaatac cgtaattgcc
    14161 ttgttatcta aaaaccactt gaaaaaccaa gataggctaa aatgtttctc tttttgctcc
    14221 ataaatactt cctttctatt gttctctcat tataccattt ttgaaacaaa ataactcttt
    14281 tttaaagtgc ttacatctaa ttgaatgtct ttaatttgct tgtttttgtg atataatagt
    14341 cttatcctta cacagaggta tagtaatgaa agaaactgtt tattttggaa cttatacacg
    14401 tcgtacttct caagggattt acaaggcaga ctttgataca gaaactggtc agctttcaaa
    14461 tctagaactt tttgcagctg agccaagtcc aacctacctt gcctttgacc agcaccaaca
    14521 tttatacact gttggtagcc aagacgataa ggggggaatt gcagcctatc aaactgacgg
    14581 gactgtgtta aatcatgttg ttgaagaagg agctccccac tgttatgttg ctgtcgatga
    14641 aaagcgtgat ttggtttacg cagccaacta tcacaaggga caagtccttg tttataaacg
    14701 ccaggaagat ggtagtcttc tacttagtga tatggatcaa cacagtggcc aaggtccaca
    14761 tgaaaatcaa gcttcccccc atgttcacta tacagattta acacctgacc actatctagt
    14821 gacctgcgac ttgggaactg accaagtcat cacctatgac ctcgatcaag aaggaaaatt
    14881 atctaagctc tatacctatc acagcaagcc aggagcaggc tcacgccata tcattttcca
    14941 taaccactat aaaatcgctt atctcatttg tgaactcaat agtactatcg aagttttaat
    15001 ctacgatggc gttggcgaat ttgaacgtat gcaggtcatt tcaactttac cggaagctta
    15061 cgaaggcttt aatggaaccg ctgctattca tctctctaaa gacggtaaat acctctacgc
    15121 ttctaaccgt ggccatgatt ctatcgcagt atataccatc cttgcggacg gtagcttaga
    15181 gttattagaa atcgttccaa cgcatggtca gactccacgt gattttgatt tgacacccga
    15241 ccaaaaattt ctcattgttg tccatcaaga ctctgacaat gcaactgtct ttaaacgtaa
    15301 ttgtgacaat ggtcgtctag cagaactctc caacgacttc catgttcccg aagcagtctg
    15361 catccgtttt gctccttaaa acaccataaa aaatgaactt ggtattttca agttcatttt
    15421 ttcttatagt ctatttccga cattaatacg gttaatggca cgttgcaaag caatcttagc
    15481 acgacgttct tggtcaatca aatgtttgtc ttgtgcttct tcaattgcac gttctgcacg
    15541 aagtttggca cgttctgcac gactgatatc gatatcacga gcacgttctg cagagtcagc
    15601 gacgattgta atcatatcat tggcaatttc aataacgcca ccgtttactg caatccagtt
    15661 cacgtgatct ttatcatcga tacgttttac ctttacttca tcaactgcta aaaccgcaat
    15721 catattttca tgtcgtggca agatccccat ctcaccatcc agagttcgaa ccgatacata
    15781 gctggcatgg tgatcataga cgagaccatc tggtgtcacg atctggacag ttaactgagc
    15841 cataggtcac ctcttaaaat cccatttttt cagcttttgc aatcacatct tcgatagaac
    15901 ctacaccacg gaaggcatct tctggcaagt ggtcgtattt accatcaagg atttccttaa
    15961 agccacgtac agtttcagca actggaacat aagaacctgg ctgaccagta aattgttccg
    16021 caacgttgaa gttttgtgac aagaagaact ggatacgacg ggcgcgagca accaaggtct
    16081 tttcttcatc agaaagctca tccataccaa ggatagcaat gatatcttgc aattcatggt
    16141 aacgttgaag gacacgtttt acttcagcag caactgcata gtgctcttct ccaacgattt
    16201 caggtgccaa ggcacgtgag cttgaagcaa gtgggtcaac ggctgggtag atacccaatt
    16261 gtaccaactt acgttccaag tttgttgttg aatccaagtg agcgaaggct gttgctggcg
    16321 ctgggtcagt atagtcatcc gctggcacat agatagcctg gatagaggtt acagaaccct
    16381 tcttggttga tgtgatacgt tcttgcaatt gacccatttc cgtagcaagt gttggttggt
    16441 aaccaacggc tgatggcata cgacccaaaa gggcagatac ttctgaacca gcctgagtga
    16501 aacggaagat attatcgata aagagaagca cgtcttggcc ttccacatca cggaagtatt
    16561 cagcgattgt caaaccagta agggcaacac gcatacgtgc tcctggtggc tcattcatct
    16621 gaccaaagac catggctgtt ttctcgataa cgcctgattc tttcatttcc cagtaaaggt
    16681 cattcccctc acgagtacgt tccccaacac cagcaaatac tgaaatacca ccgtgctctt
    16741 gggcaatgtt gtgaatcaat tcttggatta agacggtttt accaactccg gcaccaccga
    16801 aaagtccaac tttaccacct ttaaggtaag gggcaagaag gtcaataacc ttgatccctg
    16861 tttcaaggat ttcagaagag gtagacaact catcaaaagt tggagctttt ttatgaattg
    16921 gctgacgctc tgcgtcttct gtaaaaggag cttccaaatc aatggtatct cccaaaacgt
    16981 tgaagacacg tcccaaagtt tctttaccta ctggtacaga gattggacga cctgtgtcca
    17041 atacttccat tccacgagtc aacccatctg ttgattccat ggcgatagta cgaaccatac
    17101 catctcctaa ctccaaggct acttcaagga cgatttttgt ttttctttcg tcatttttgt
    17161 agacgacaag tgcattatta atctcaggaa gtttttcccc tgctgcaaac aaaacgtcta
    17221 caacgggacc gataacctga gcaattttac ctgaactcat ctccttctcc tattctatat
    17281 aagatactgt cggttcctag ctcaactagg tcctaagttc attttcatac gagctggact
    17341 agagcctatt ctaaggcact agcacctgct acgatttctg taatttcttg tgtaatagcc
    17401 gcctgtctgg cacggttata ctgaattgtc aaatcattga tgactttctt agcattatct
    17461 gtcgctgttt gcatggctgt catacccgca gcattctcag ctgtcttggc atcgataatg
    17521 gcaccgtaaa tcatactttc tgcaaactga ggcaacaact gctccagaat ttcttctcgg
    17581 ctggtttcca attcaaaagt caagctgtac tcttcatccg cttcatttgg atccaagtca
    17641 acaatcggaa gcatttgttc cacacgcatt tgactggtta gcgtattgac atggtggttg
    17701 tagcaaacat aaagctcatc aaagagttca ttttggtaca tttcaacagt ttttgaaata
    17761 atcttacgaa cttgatcaaa gctaggttgg tctgacaagc cacgtaattc ataaagtggt
    17821 tgaataccgc gagccttaaa gaaatcagct cccatcccac cgatacagat catttcaaaa
    17881 cctttaccgt ctgggtggta ttcttctttc aactccataa cagctttcaa aatagaggaa
    17941 ttataacctc caaccaaacc gcggtctgaa gtgataacga tatagcctgt cttcttcaca
    18001 gaacggctaa tcaacatcgg attagttgaa gcaccagctc cattaccatg aaggatatct
    18061 gtcaaaagtt tacgcacttt ctgagcgtaa acttggaagt tgcgagcagc ttcttcagag
    18121 cgacctagct tagcagccga taccatttgc atggcattag tgatttgact cgtatttttt
    18181 gttgaggcga tttttgtttt aatatcattt agagacactg ccatctgaca cctctattct
    18241 tattggaagc tagattgatt gagaaactct gtaatcgcag catccaagac tgcttcttct
    18301 ggcaagtctt ttgtatcacg aatggtttcc aaaatctctg gatgttgagc atcaaagaag
    18361 gcatggaact cttcctcgaa acgaacaata tcatctactg gaacagtatc caagaaacca
    18421 tgtgtcaaag cataaagaat ggttacttgt ttctcaacag gtaatggttt gtgaacaggt
    18481 tgtttcaaga cctcaacggt acgacgtcca cggttcaact tagcctgtgt tgctgcgtcc
    18541 aagtcagaac caaacttagt aaaggcttcc aactcacggt atgaagcaag gtcgatacga
    18601 agtgtaccag caaccttctt catggctttg atttgtgcag aaccacctac acgagataca
    18661 gatgaacccg catcgatggc tggacgaata cctgcattga agaggccatc gccaaggaag
    18721 atttgtccat cagtgataga aatcacgttg gttgcgatat aggctgagat atctcctgct
    18781 tgtgtctcga taaatggtag ggctgtaatt gatccaccac caagttcatc agaaacttta
    18841 gctgagcgct caagcaaacg gctgtggaga tagaaaacat cccctgggaa ggcttcacga
    18901 cctggaggac gacgaagcaa gagcgacagt tcacgataag ctactgcttg ttttgataga
    18961 tcatcgtata caatcaaaac atgcttacct tgatacataa attcttccgc catagcaacc
    19021 ccagcataag gagctaggaa gagcaatgga gatggttgtg aagcagaggc tgtcacaacg
    19081 attgtgtagt ccaaggcacc gtactgacga agtgtttcta cttgcgtacg aactgttgat
    19141 tctttttgtc caatcgcgac gtagatacag atcatatctt gatctttttg gttcaagatt
    19201 gtatcaatcg caatggttgt tttccctgtc tgacggtcac cgataatcaa ctcacgttga
    19261 ccacgaccaa tcggtacaag ggcgtcaata gctttcaaac cagtttgcaa tggttctgaa
    19321 acagacttac gttgcataac accaggagct ggtgcttcta ctggacgagt tttatcagtg
    19381 tggatttctc caagaccgtc aactggacga ccaagcggat ccacaacacg accaatcaga
    19441 ctttcaccta cagggacttc catgattttc cctgtacggc ggattgtatc gccttcacgg
    19501 atatctgtaa agtcacctag gatgataata ccaacgtctg ttgactccaa gttttgagcc
    19561 ataccataag agccgttttc aaaattcaac aactctccac tcatgacatt ttcaaggcca
    19621 tgagcacgcg cgataccgtc cccgatatag gttacaacac ctgtttcagt cacatcaaaa
    19681 ttgggtttga aattttcaat ttgttgctta attaaagcgc tgatttcttg tgcgttaatt
    19741 gccaaaagaa caccactttc tatttcaaat tttctttaac aactttaagt tgttgtttaa
    19801 tactcacatc aattgtcttg tgattggcaa aaatgacaaa accaccaatg agactttcat
    19861 cgatttgttc ttttacactc cttactttca gagacatttt tttctcaatc aaagggagca
    19921 aacgagtctt ctgttcatca gttagaggat gagcagacgt aatcgtcact tcaaatcgat
    19981 ttgtttcttt ttcaagtcgg ttcaagcaat ctacaagcac atcataaaaa agatttgctc
    20041 tgtgattgta ggccagaacc tggataaagt tttgtaataa aggcgacaca gaatcttgga
    20101 aaaaagcaat tgttttttcc ttatccgact cgtctactgc cacttgtttt aaaaaagaag
    20161 gcagacctgt tttttcaaca acttgcttga tttgagtcaa gtctgaaaag atacggtctt
    20221 cttctccttt ttcaagtacc aattggacaa aaggcatgct gtatttttca attaccttta
    20281 ctgttttctt gtccattaag cttctcctag ctgatcgata tactgatcaa tgagtgcttt
    20341 atgggcatga ctgtcaaggt tttgtgagat gattttacca gctaagctga tggtcaaatc
    20401 tgcgacctca cccttaacac tctgtaaagc ttctacttta ttttgagcaa tttcttgatt
    20461 ggctttttct tttaagtgtc ctgcttctag tttagcatct gctaagatat tagccttact
    20521 ttgctcagct gtttcctttg cattttcaat gattgtctta gcttctttac ggctaccagc
    20581 caattcatct tcgcgttttt gagccaatac ttctgctttt tgacgggctt cttcagctct
    20641 gtcaatatct gaagcaattt tttcagctct ttcttcgaaa atgcctgtaa tattagacca
    20701 tgcaaatttt ttaatcaaga ctagcaaaag aataaaagag ccagtgatta aaataaaatt
    20761 accaattaat tcacctactg ttacgtgcat cagttactcc tttctattcc tcttcaatat
    20821 taatcttatt ccctaaatac acagatgtca aaagagtaaa aacataagct tggatgcagg
    20881 aaataaagac agaaaatgca gtccaagcca aattagctcc aaaggctact ggataccaat
    20941 aaatagcttg gtgggaaaga agaagtaaca aactcgtcat gacctctcct gcaaagatat
    21001 tcccaaaaat tcgcaaagcc aaagataaga agtttgtaaa ttcttccaag atattcatcg
    21061 gtatgacaaa aacaggactc ataaaagatt ttatactttt tttaaatcca cgacgacgaa
    21121 cgctttctat atgtgtcaac aaaatgacaa gaaaagataa ggttaagtca tactgtaaat
    21181 tagcggttgg cgaactccac cagttagtcc catcgatcgt ttgaatcttt gtcattaagc
    21241 caaggttatt ggcaatcacc atgaaaagga ataaacaaag gaaaaagagt gagtaatctt
    21301 tcatgtagcg cgaaccaatg ttaggttctg taaatccaat aacaaagtca tagacatact
    21361 caagtacatt ttgctttcct ttgggtttca aggtcatatt gcggcttgcc caataaataa
    21421 agacaaaaat aactcccaca atcaacaaag tcatggctaa catagtcaga ttgaagataa
    21481 caggaccaat agagatgatt ggattaatac tttcttccat ctaaaatcct cccttttctt
    21541 ctcgttccat gtatttattt gataataaat gagaagacaa gagttacaaa gaaagttcct
    21601 tcaataaagg caacacctaa aaacatcaaa ctacgaaact cagaaagcat atctggttgg
    21661 cgagctactg atttaaacag tccattcatc aataaacctt caccgacaga tacgcccata
    21721 caggcaatac ataagcctaa aaatgttaaa ttcataacaa attctccttt aaaataaaat
    21781 ttacttactt agtgtagtcc taaaatttaa atttgtcaac ttttaaccgc tattggaagc
    21841 gtttaataaa aattatttcc attttgctaa atttcaagtt caagttagcc atccagaagt
    21901 cttctctggg tgacttgtag tccaagcatt ttttaggata gttgttaatc cacttttcga
    21961 tgaatgcgac ttctttggga gtcattttct tggttccctt aggtaaccat ctacgaatga
    22021 gcctgttgtg attctcatta gttccccttt cccaagaggc atagggatgt gcataataaa
    22081 tgtgctcctc agaaaataca ttagacaagc gattgaattc cgttccatta tctgccgtga
    22141 tggaaagaat cttgtgttgt tttaagatga gttttagagc ctgattgacc acctcagcac
    22201 ttttatttgg aatcaatcgg atgatctgat gtctactttt tcgatccgtc aagacaagca
    22261 agcagtagtt tttcgctctc gtaagtagaa ccgtatcaat ctcataatgc ccattctcca
    22321 agcgaaaatt gatagcttca ggccgctgtt cgatggattg accagcaggt ttaaagttgg
    22381 tgctggcctg tttcttaagc gcttttcctt ttctagggta aagcagatcc tgcttgcttt
    22441 accccaattt tccatgatga atccaatagt aaatggttga aattcccacg ttaacccctt
    22501 tagccataac catcatttca ggcaaaaatt tttggttatg atagtggaga atcttttcct
    22561 ttagttcctt ggtcacgctt gatctcttga ccgagtgctt gcgattattt tcataagact
    22621 gttgagcata gtcggcagaa taaacctctt tgaagcgccc ttttccaaga cattttcgga
    22681 ctgtcctacg cttgatttta gtgtggatag tttgaggagc ttttccaagt agagaagcaa
    22741 tttctctatt tgattttcct tcttttttcc atctttcgat taagcgacgg ctatcgattg
    22801 tcaaatgttt accttttgta gtataattgt cttgcatttc tgtgcctttc ttgtgtttgt
    22861 ggttgaacaa caagtataac acagaggtgt tttcttatgc ctacaagagc tttcattatt
    22921 tccattttct tttggatttc actctattct gaaaaacttg tgtatattta ctgaagctag
    22981 caagtcttac ctgtaaattt aatgaaagca acacaaaatc cgagagggga atctcggatt
    23041 aatagataga gagtttttag tttaaataaa ttgtttaaaa tatcaacaac atcacttctt
    23101 ttcttaacct gataagtctt gattcctaat tttggggcta cgattatatt gtcctcaata
    23161 tcgtctagaa agacacaatt tctaggttat aactggtatt tatcgatagt tactcatata
    23221 catcagtcca cctccatact tatgtgcgag cctctctttg tattatacct ccatactcac
    23281 cttacagatt cttttggtaa taatatcttt gcctaatgta gagatagtct tgcaaagaaa
    23341 aaacttcctt gtagccatgt ttctgataaa agtccggtgc ctggaactgg taagtattga
    23401 caaaggcaaa acaacaattt cgattcttag cttcactttc tgcctgttgc aatagttttg
    23461 aaccgattcc ttgtcctcgc agttcctctt ttacaaacaa atactcgatt tctagccaat
    23521 ttccaaaagt ctctgctatc aaacctgcca ggagattgcc cttttcatct tcgacataaa
    23581 gattaagtgg ctcactttca gcctcttctc tttttgaacg gttataaaca cgaatcagat
    23641 tccctatttc ttgcgattta tgtgattcct tattttccaa tctaaagtat agtgaaatga
    23701 aataaaacat gcgcaaatcg attaaggaat ttaatctaat ttctaacaat gtcttagaaa
    23761 tcaaagtgta ctattttaac ttcaatgcac tatacatcta atactcaata aaaatcaaag
    23821 agcaaactag gaaactagcc gcaggttgct caaaacactg ttttgaggtt gtagatgaaa
    23881 ctgacgaagt cagtaaccat acatacggca aggcgacgct gacgtggttt gaagagattt
    23941 tcgaagagta gcaaaatgga aaaaggagtg agtgaagcac atcgcctccc cactcctttt
    24001 tctgttttta ggctgttttt tcaaccttca agatttttac atcatagcta ccaacaggcg
    24061 tttcaatggt tgctgtatca cctgttttct tgccaatcaa ggcctgccca attgggcttt
    24121 catttgaaac cttacctgca aaggcatccg caccagctga acctacgata atataaactt
    24181 cttcttcgtc ctcaccaatt tcttggatgg tgactgtttt accaatcgct acttcgtcct
    24241 gggcaactgc gtcgctattg acgatttcag catagcggat ttttgtttct aagctagaga
    24301 tttgtccttc gacaaaggct tgttcatcct tagctgcttc gtactcactg ttttctgaaa
    24361 ggtcaccgta tgaacgggca atcttaatgc gttctaccac ttctggtcga cgaaccaatt
    24421 tcaattcttc taattctttt tcaagttttt ccttttcctc aagggtcata ggatatgttt
    24481 tttctgccat ttttctcaac tttcttctga taatattttc taaagaaaat tatgtgaagt
    24541 atcacataat tttagtttgt ttagtttaat ttgctgttga catgttcagc gacattgcgg
    24601 tcgtggtctt cttgattgtt agcatagtag accttgcctt ctgtgacatc tgctacaaag
    24661 tagaggttat cgctcttagt ttgattgatg cttgactcaa tcgcatccag acttggacta
    24721 tcgactggac caggcatgag acctacattt ttataaacat tataaggtga atcaatgttg
    24781 gtatcaatcg caacatcctc agctagactg atattttgcc ccagttttcc ttgggcatac
    24841 aagattgcaa tattactttg aagtggcata tcacgattca aacgattgta gaatacacct
    24901 gcaatgagct tacgatcttc tgtcttggca ccttcttttt cgaccaagga agcaatggtc
    24961 aacaactcat tgacagtcaa gtttttagat ttgatagtac tatagtaagg agataggttc
    25021 ttatccatag cagctaacat ctcatcaatc aagctctcaa tagttgtgct ttccttgata
    25081 gagtatgtag ctgggaaaag gtatccttcc aaacgataac gcgcaccgct gtcttttaca
    25141 ggcaaacttt ccagtaaagt aggatatttc gctactgctt gactgataaa cgtctcatct
    25201 tgaactttag ccaagaaagc ctccgctgtc aaagactctt tgaagtcacc ttgcaattga
    25261 cccacagttt gagcaatctg atccaaggta taaccttctg gaattgtcaa agtcgcaagt
    25321 acaggttctt gcggttcatc tgttccacct ttttgcaact ctttgagtaa gtcttctgta
    25381 ctcatactct tttgcaaatt gtagtaacct gctttcaagt cggtataatt tttatacttg
    25441 gcataaaaac taaaaatcag accatgcttt atcaaaccag ctttttcaag cgtcgtaccg
    25501 atttcttgaa cgtttgaacc ttctggaatt ccaaccgtca catatttctt agaattagca
    25561 tcgataggta ataaggaatc tagcacgtac tggtaaccaa agtaaccacc tgctgagagc
    25621 aaggcaagga atacaaggaa tgaaatcaca aaagctttca catgtgactt cttctctttc
    25681 ttaggcttaa cgggttttgc tccttctcta cggctacgac gtgatgtatc tctgatgata
    25741 tccactgttt ctgtagcagg cgtaggtgtt tctggacctg cttgttctgc ttttttctct
    25801 gccgtcgtat aggaaacagc tacccttgtt ggggtttcat tgtattctct ttcaagtttc
    25861 ttaggtctaa caggacctgg acctggtctt gatccacttt cttccgctgg agaagaaggt
    25921 acatcttgac ttggatgact tggaacacca ggagtttctc tttgaatctc atctgctgga
    25981 gaagctggta caccttgact tgggtgagta ggcacggtag gagcttttct cataatctcc
    26041 tctaccgttg acaaggaatc agccatgagt tcttcagctg aaggttcatt tgcaggagtg
    26101 cgaactactg cctcatcttc tttcagaact tcatcatagc cttttacttt ttctaaatct
    26161 ctcagaatct gctctttaaa gcttaatttc tcttcttctc ttgacttttc actcaaaagt
    26221 ttttcctcct tgttgataat ccataatatt atatcttaaa agtccaaaaa aagcaatcta
    26281 tgatactttt cctaacttat tttttcattt cccagaccat atcataccat gtttcccctg
    26341 caaaggttga ctgggactgg ccttcgttga caaatccatg tttttcataa taggcgatga
    26401 gatagtcatg acaggttaga ttaatgccat ctctctcatc ttcaagagca acttctttca
    26461 aggctgtcag caatttctga cccagtccga gtccctgtgc ctccttggca atagacagac
    26521 aggtcacaga gatataacca ccaggctcat gactatagtc ttttatttct tctgtaaaag
    26581 actggtcttg cagatggcgg tgcaggccaa ctggtccttc gatataaccc atgattcttc
    26641 cttctttttc agcaaccaga aaagaggtct gaatttctct caaatgtgct tcaaagacag
    26701 aaggaggaat ggcttcttcg accgaaaaat tttcaaattc aagttcaaca atccgatcca
    26761 aatcttctaa tcttgcttgt ctgattttca ttgttcctcc agataaaagg gattaaacca
    26821 aatcatacta tagccctggc tagttacata gagcaaagtt tcttcttcat caacaaaacc
    26881 gttcatttca aaataggaaa gcagctcatc aggactctcc aaacgaatcc ctttgtaatc
    26941 cagctcaact gccacctctt tcaaggctgc aagaagaagt gttcccaggc cctgtctctg
    27001 atggtcagac tcgatgacta aagaatgtac ttttagacat tgcggattgt ctgactgggg
    27061 acttgataaa atatagccta aaagttgatt ttcatcccta gctagaagaa aggtatccgc
    27121 acacttacgg atactttctt ctaaaatatg ggaaagttgc tgcttttcag ctggaaaaga
    27181 cgaggtctga agtgccccta tctcaggcaa atcaaacttg cttgcctgaa tgatcttaat
    27241 tggaatttcc atgggaacat cctattgaac attgcttgtc aagttagaca agagacgctc
    27301 aaatgagtat tcataggttt ggatgtctcc tgctcccata aagacgtaaa cagcattgtc
    27361 atggtctagg agtggagaaa cattttcaac agtaatcact tggtgttttt tgttgatttt
    27421 attggctagg tcttctacct taacgtcacc atgatctact tcacgagccg agccataaat
    27481 ttgcgctaga taaacagcat ctgcttggtt taaagcatgg gcaaagtcgt ccaacagggc
    27541 aatggttctt gtaaaggtat gcggttgaaa gactgctaca atttccttgc ttgggtattt
    27601 ctgacgagcc gcatccaagg tcgcaataat ttctgttgga tgatgggcaa agtcatcaat
    27661 aatcactgta tcattgacaa ttttctcagt gaaacgacgt ttaacaccgg caaatgtttt
    27721 caagtgctca cgcaccaagt tcaaatcaaa tcctgctgtg taaagaagac caataacggc
    27781 tgtcgcattc atgatattgt gacgaccaaa ggttggaatg tggaattgcc ccaagttttg
    27841 tccacggaaa tgaacggtga aggttgaacc agttgttgaa cgaagaagat cactagctac
    27901 aaagtcattg ccttcagctt caaaaccata ataataaatt ggtgcatcag acgtaatctt
    27961 acgcaattca gcatcttcac catagacaaa aagacccttg gtaatttgtt tggcatagtc
    28021 gttaaaggca ttgaaaacat cctcgagact tgtgaaataa tctggatggt caaagtcaat
    28081 gttggtgata atagagtatt ctgggtggta aggcatgaag tgacgctcat attcgtcaga
    28141 ttcaaagaca aaatatttgg cattggccga accacgacct gtcccatctc caatcaagaa
    28201 gctggtatct gtaatgtgag acaagacatg agacaacata cctgtcgttg aagtttttcc
    28261 atgtgctcct gctactccca tgctaacaaa gtcacgcata aagctaccta gaaactcatg
    28321 gtaacgtttg tagctgatac cattttggtc cgcataggca atttcgacgt tgttatctgg
    28381 acgaaaggca tttccagcga taatttccat atcaccgtct agattctttt catcaaaagg
    28441 aagaatggta attcctgcct gctcaagacc gcgttgggta aagtagtact tttcaacatc
    28501 tgatccctga accttgtgcc ccatctggtg caacatcaag gccaaggcac tcatccctga
    28561 tcccttaatt ccgataaaat gatatgtctt tgacatgttt tctcccctat tctgtcattc
    28621 tggtcagatt caactcttgg gcaacccgac gttcttgttc tgtttgttta ctttttttat
    28681 tgtagatttg gctcttcttt agaaaatcat aattgttttt ctttggagca ggtgctgaca
    28741 cttcttcatt cttggtaggg atagaatgaa cttcttccgc caagatataa tgagactggg
    28801 tcaatttttg gctatatttg acaaattcac caggattttc cttttggaaa ggagttgtcg
    28861 gttgattgcc ctgtctaact agactgggct gagaatgacg tctcgcaagg ctgaaatcct
    28921 gagttaggta gttagcagag cgtttctttt tcaagtccgc acgcgcttct tcacgcgcca
    28981 cctccgcata gctctttcct tcttttttaa cccctaaagg agccttttta ggtttttcga
    29041 cttgcttttc aatcggtttt actggttttt cttcagcaat aggagcccat tctaaataat
    29101 ttttatctcg atactcaccc ttgatattac tgatcagatc agactcatca tagagattca
    29161 tgactggcat ttcagtcaac atgacctcgt catctgacac caatggaaat cgttcttgtt
    29221 tcattttcta tttcctttca acacttcatt atagcgtatt gtcttgattt ttcaagtgct
    29281 ggcttcagaa attcccaaaa tttctctaat ttctgctagg gtcagactac cacgtgactc
    29341 tgtgccgtcc aatacttgtg acaccagatg tttcttttgt tcttggagtt cctgaatttt
    29401 ttcttcaatg gttcccttgg tcaccaagcg atagacctca accgtttctt cctgacccat
    29461 ccgatgggca cggccaatgg cttgcgcttc caccgcagga ttccaccaaa ggtcaaccaa
    29521 gatcactgta tctgcacctg tcaggttcag accgacccca ccagccttga gggaaatcag
    29581 aaaggcatct ctttctcctt ggttaaaggc cttggtcatg tcttgtcttt ccttggctgg
    29641 ggttgaaccc gtaattttaa aggaagtcag gcccaagtct ggcagttctt gttcaatttt
    29701 ttccaacatt cccttgaact gagagaaaat caagacacgg tgtccgccgt ctgccacctg
    29761 taccagtagg tctcggagac tatctagttt gccgctggct ccctgataat cttccataaa
    29821 cagggcagga gtgtcacata tttgacgcaa gcgcatcaaa ccagataaaa tttccacacg
    29881 acttcgctga aattcctgtt ctgacacttg agccagatgg tctcgcatct gttgtaactg
    29941 ggcaaggtaa atagcctttt gctggtcttc cagttcattt ttataaacca cctcaatcaa
    30001 gtctggcaat tcagtcagaa cttcttcttt cttgcgtcgc atcacgaaag gcttgataaa
    30061 ctgagccact cgctctgctg gcaatttcat aaattctttc ttgcttggca aaagtccggg
    30121 catgacgatt tggaaaatag accacaactc acccagatgg ttttcaatcg gagttcctga
    30181 caaggcaaag accgacggca ccacaaattg tctcaaggtc tgggcaatct tggtctgggc
    30241 atttttcatg acctgagctt catctaagaa aaggaagtca aaggccatcc cctgataaag
    30301 ctcactgtcc tgacggaagg tggcatagct agtcacatag atttgatggc tatcggcaag
    30361 aatctcctca cgacttgctt tcaaaccatg aacaacagcc acatccaact gtggggcaaa
    30421 tttctgaaac tcatccgccc agttgtaaat caaacccgac ggagcgagaa tcaagactcg
    30481 actttctttt gtcacttgac tagtcaaaaa agcaatggtc tgaagggttt tcccaagtcc
    30541 catatcatca gccaaaatcc caccaaaacc ataatgatgg agcatctgca accagccaat
    30601 tcccttttcc tgataatctc gcaagtcagc cttgacctga gttgcttgca aaggaaagtc
    30661 ctctggatgc gtcaaatcct gggccagatt ctggaattct tgtgaaaaag aaacacggtc
    30721 tcggtctcgc ccttcaaaga gatgagctaa actgtaggcc aaggatttcc gagcctgcaa
    30781 ggtcccatct tttaattcaa attgccccag tttctgtaaa ttttggcgaa ttttcttggt
    30841 ttcttcatcg aaaaagtaaa cttgattaga cgaatcaata taaaaatcct gattggcaac
    30901 caaggcctgc atggcttggt cgatttcctc ctgggcaata tcttgaaaat caaactggat
    30961 ttccaagaga cctcccttgg aggcaatctg cacctgagga ctcgctaggc tataaagctc
    31021 ttctagttta tctgataggt caacatgccc gagtttttca aagactggaa tgatatcatg
    31081 aaaaaaatga tagacagact ctgcttttaa ggcctgacgc caagattgaa aatcggcttc
    31141 aaagcccgca gccaaacaga cttggaaaat tctttcttct aagtccacgt cacttgaaaa
    31201 tggtaattct tctagctctt gtcggctaga tacctgtcta tttccataat caaactgaat
    31261 ttctaaacga atccgattat cttcttccct gtcaaagtaa aaagagggcg caaaagtttt
    31321 gatttgtaga cgttctggag ctgaaacggt gcccatctgg ataaaaagag tcagacagga
    31381 ggccaatttg tctcgatcac tgctatcaaa ttgcaggtat ttctttcctt gttgacccac
    31441 aggtaacact ttaatttcct tgagaagacg catctgctgg tctgttaaaa aataaacctg
    31501 acctttatgg aaaagtactg ctccctgata aaagacattg accctaggac tctcactgat
    31561 ttccatttca aaatagtcca agtattctgt tactgtaaag gcaaatagat tggtatcagc
    31621 atgcatatcc tgaaaaagca gggtttggta gctatccact tgatggtcaa attgaaaatg
    31681 gggcaaggcc atcagtaaat tcacaccctg ctcaaaaaag gtcagaggga aaaagaggtg
    31741 ccgaccttgg ttttggaaaa agaggtctgg aaccagccct tcctccgtta gtccgtgcaa
    31801 gaaagtcaaa agttcttggc tggcatcatc aaaggcttcc caagaaagag actcctcata
    31861 aatcttgcca atcatatacg actttctctg ctcgacaatc cttaaaaaaa gtggaatatc
    31921 ccgaatgaca tagtattttt gtctattgat ttggccgatt ctcagagtcc acaagatatg
    31981 attggttcct gcttccacct gacccacagc tgataactca taggcgcatt ctgatttcgg
    32041 agataaaatt cgattcaaaa acttgccacc caaggtcacc ttggtttcaa cggcctcttt
    32101 ttcttcatga ccttcttcca gactccacaa gatttcctga ccacgctcat catttttcag
    32161 aaaatgctct agcgctgcca aatgcacaca gtagcccctc ttttgaaaag aatcacaggc
    32221 acaaaaaacc aaatcatcct ctaaactata gcgcagttct tcttctgcaa cgcgagcgta
    32281 gagccgattg ttcttttcct tgatgatatc aaccttacca gtttcataaa gggcaacacc
    32341 ttcgatacga actttccccg gaatcaattt agccatattt tcacctttac cttatctgtt
    32401 tattatacca tattttcgcc tatgaaaata gccttctagg aagacttttc tcctagaagg
    32461 ctggattttt aacgtttggc aaaagtagcc acaatccgct gacagacttc ttgcaacaga
    32521 gatttgggca tagctatatt gatgcgggaa tggagacttc cttcctctcc aaaatccaaa
    32581 ccacggttga ggataacctt ggcttcattt ctcaacaact cttgcaatgt ttcatcagtc
    32641 aggtcatagg ctgaaaagtc aagccaaatc aagtaggtac cttgcggttt catgaccttg
    32701 attttagtct cttttccaaa tagatccacc acataattga tgtggtcttc aaagacttgc
    32761 ttgagttcct ctagccaatc tttaccgtat ctataggcag cttctgtcgc caaataaccc
    32821 aagcctgaaa tttcatgctg attattggcc aacaggcgtt tctggaaagc tagtctcaac
    32881 ttaggatttt caatgactgc ataggaattt tttgttccag caatattaaa tgttttagtg
    32941 gcactgctca agacgatagc aaaatttttg aaggcaggat tgatggtatt gaaagactgg
    33001 tgtttgtgac caaagagggt caaatcttgg tgaatctcat ccgaaactaa caaaacaccg
    33061 tgtttttggc agagttggcc aatcttctcc aacacttctt tttcccaaac acgtccacca
    33121 ggattgtgag ggttgcaaag aatatagagt ttaacctcct cttccaccaa atccttttca
    33181 agttggtcaa agtcaatctc aaacagacca tccttttcca ctaaggaatt agtaatcaat
    33241 ctacgattat tcaacttgac actgcgagca aagggtgggt agacaggcgt gttaattaaa
    33301 accgcctcgc cttcttttgt aaaggtttga atagctgttg agatggctgg taccacaccc
    33361 tcgataaaga caagagcctc tttgtcaaag ttgtaaccgt attgtgtagc ttcccacttt
    33421 tgaacttcct taattaagtc ttcactggca taggtataac cataaaccag ttggtctgcg
    33481 taagtttgca cggcttggcg gatttcaggc aagaccacaa agtccatatc cgctatccaa
    33541 gctggtagaa cttcactatc cgtttctgtt tctttccatt tataggtatg gtgccctaaa
    33601 cggttgggca ggcttgtaaa atcatatttt cccatctttg tcttatcctt ctaaggcttg
    33661 gcgcaaatct gcaatcaaat ctctagcatc ctcaatccca atagacaaac gcaagaggtc
    33721 atctgtcaaa ccataagaat ggcgtacttc tgctggaata tcagcatgag tttgagtcgt
    33781 tggataagta ataagacttt ccactccgcc caaactttcc gcaaaagaga agaccttgag
    33841 actgttcaaa atatgaggaa tgcgtgtttc atcggctact ttaaaggaaa tcatgcctcc
    33901 acgaccagtg tagagaactt ccttaactgc tggagaatcc ttcaaaaagg caaccacttc
    33961 ttgggcgtta gctgttgagc gctccatacg aagagacaag gtcttgagac cacgaagcaa
    34021 ctggtagctg tcaaatggag acaagactgc ccctgttgta ttgagattgt aaaaaagctt
    34081 ctcgtatagt tctaaactat tggtcacaac cactccagcc aagacatcat tgtggcctgc
    34141 tagatacttg gttgctgaat ggagaacgat atctgctcta tcttcaatcg gacgttggta
    34201 gatagggcta tagaaggtat tgtccaccac cactttggca cccttagcat gagccaattt
    34261 tgctagtttt tcgatatcaa attccaacat caagggattg gttggggttt cgatatagag
    34321 aacatccaca tccttttcta actcggcaat caactcttct tctgtattgg cataggtaaa
    34381 atggaaacga ccttcctgct ccacttggtt aaaccagcga aaagaaccac cgtaaagatc
    34441 acgcactgcc aagaccttac ttcctactgg aaagacgcta aaggccagta caatagctga
    34501 catccctgag ctagtcgcta aggcatagtc tgctgactca atagccgcca agacttcctc
    34561 agccttacta cgagttggat ttttagtgcg cgtatagtca aacccagtag atcgaccaaa
    34621 ctctggatgc tgataggtcg ttgaaaaatg aagcggtgtc accaatgcac ctgtcgcttc
    34681 atctgactta atacccgcct gggccaaaat tgtgttaatg tgtaattcct tgctcataca
    34741 atacctccaa atctatagta actattgtac cacttatttt catcaactca ttttcttctt
    34801 ttcaagagct agttatagtt tcaaactata taaaaaggga gtttttcctg ctccctttaa
    34861 tagactataa aatggtgaat ctcaaaagac acttcactct atcatttgct cctgcacaaa
    34921 acgagcataa cgctcatgat tttccagtag ttccttatga gttcctgagc cagtgatttt
    34981 cccctcctct aagaagaaaa tacaatccac atcttttacc gttgacaaac tatgcgctat
    35041 aatcacaacc gtcttctcct ttaatacaga atagaggcta ctgataatcg catactcaga
    35101 atccgcatca agattagcag tggcttcatc aaatataaga atttcagcat cttttaagta
    35161 ggttctagct atttgaagtc tttggctttg cccccctgac aagaatcgtc cgcgttcacc
    35221 aacttcagta tctagtccct ctttcatgga gcgaatctca tcacctagtg atactaagtc
    35281 tagcactttc atcaattcat catcagttac taagcgattc aaaccgagac aaagattgtc
    35341 acgaatactg ccagataaga ctgcattatt ttgtgaaacc caagcgattt tacttctcca
    35401 ttcttttaag ttaaaatcat atatacttga ttgctccatt agaatatctc ctgaaagcgg
    35461 tttataaaac cgctctaaca aacgcacaat cgttgatttt cctgatccag atggtccaac
    35521 aaaagcaatt ttttgcccct tgaaaattga acaagtaata tcctttaaga caggtcgatt
    35581 ttcatcataa ccaaaataga catggttaaa attcaaccct tgtcctgata ccgattttcc
    35641 tcactcaaat ttttctttag gaactgcaag caagttctcc agtgcaactt aagatccctt
    35701 gctcctagaa taaacagtta caaaattagc tatattacta ataggattaa gtaattgaaa
    35761 gaggtaaatc aaaaacgaaa ccaaggttcc cacagatata tatcctgcgc tgacccgata
    35821 actcccatag gttagcatca cagctatagt cgcaaagata aataagagag caaacggggt
    35881 ctcaaaagaa gtaaccctat ctgatttcag tgaattgttt tgtacccttt caatacaatt
    35941 atccaaaaca ttctgtacac ttttctctgc ttggttagtc ttaattaatt catgttcttg
    36001 aatcttttca gtcaattgcc ctgttaaaaa tccgatttcc ttctgtaaac gacgactata
    36061 cttttcactg atattggaaa ggggcaagat aataaacatc atacaaggaa gagtgatgaa
    36121 taaaagtaga gaaagattcc aatcaagact aaataagact acaatggaac caagtaccat
    36181 aactaaactc agaataatat ttgggaaagt cgtaattaaa aactcacgaa tgacactcat
    36241 gtcattgaca atggcagaag tcaactcccc actttggctc ttatcaaaga aggatttctc
    36301 tgcataaatc aacccctata tcactttttt cctgattttt gctatctttt tttcacccga
    36361 ttgactaaac agatagtaac caatagaaga aaacaaggct tgaccaataa aaatcaaaaa
    36421 cgattgaaat actttggaac ctatattttc aatagaactc ccatctatta aaccttttaa
    36481 gataagggga agcaacaaag caagtagact agacagaaca aggaagaaac tccccataat
    36541 caccttagta tctactctta ataattttaa tttcataaat actccttata atatttcaac
    36601 ggataaagtc aggaataact caatttgagg ataaaatcta ataaatcttc ctataacaaa
    36661 acgcataaca tctaggattt tatatacctg atattatgcg tttttaagca caaagacttc
    36721 ttacacaaac ttatctacaa ttagatttta tttgacatgt tttgccaatt cttcttgggc
    36781 ttttttattg gattcttctt tttctttcaa ccatttttct ctggcttttg catattcgtc
    36841 tgttgtgaca atcttatctt gtactttgag gtatttatat gattcaaccc cttttgtacc
    36901 ggttaaacca taggcagcag caaatggtac ggttcttctc aatgatggtg ttcccccacg
    36961 cgaaacactt ggaagaacta aagaactatc aatcaaccaa gcttgaatat cagcatattt
    37021 ctcataacgt ttggctggat cttgctcttt attagcttct tccaacattt gagtatagac
    37081 atccagccca acttccttag ccttgtcatt ggcttctcca ggctctagtc caagattttg
    37141 tagaactcct ccactattag tattaaaaat atcgagatag gttgacgggt cttgataatc
    37201 aggtccccaa ccgccatgat ataaatcata gtctttctga gcagctgttt gagcaaagta
    37261 gcctgaactg tcaaactcat ctgatgttaa ttgctgaatg tcaatcacta cattatcaga
    37321 acctaaaaca gattcaattg attgtttgat agaactaact ccttgtatgc ctactttatc
    37381 tgttacttcc acagtcttat ccaagtggat tgggaattga acaccctttg cttcgagttc
    37441 tttcttagct tccgcaaact tagccttggc tttctcagga ttgtagtaag ggtcttgacc
    37501 atccgcaaag ttgatacctt gccattcctt accatagttg accatcttag aggctacaac
    37561 ttcaccaaag tcttttccct tgatactgac aaagtttgga ggaaccacta ggttacgcaa
    37621 aatctttgtt gcaccttctt tcccttcaga ctgagcccca taagatgttc tgtcaaaagc
    37681 aaaattgata gcctgacgga agtttttatt gagaactgct tcctgagtcg atttcttttc
    37741 aatgtcactt gttttagaag tataattgta agacttccta tctaggttaa aattaaagaa
    37801 atatgaagtt gaattttgca tactatagat gatattgttt ttgtattttt ctttaatccc
    37861 ttcatagctg gagctgttag gaaaaagacg agccgtagta taagcaccag ctgtaaaatt
    37921 acgttccagt gattcttgat cgctaccatc atagtaggtc aatttcacat cgtctacaaa
    37981 gacattctta gcatcccagt aattagggtt tttcttatat tcaatagcag attttgagac
    38041 aagtgctttc atcaagaaag gtccattgta caaaatacta gatggatccg ccttcccaaa
    38101 atcatcccct tttgatttca ggaaatctgc attaacagga aaaagtatcg ttgcaagtgt
    38161 ttttgaattc cagtaaagtt ctggtttaac caaagtatat tgaaccgttt ggtcatcaag
    38221 tgccttgaca ccgacagttg aaaagtcgct tgttttacca gtgatatagt catccaaacc
    38281 agcaacagag tcctgcacta gatacaaggc ttctgatttt ttatcaactg catattgcaa
    38341 acctgtcaca aaatcctggg cagttacagg cgcatattct tctccctcag aagtaaacca
    38401 cttggcatcc ttacgaagtt tgtaggtata ggtcaaaccg tcctgagaaa cagtccaatc
    38461 ctctgctaat gatggaataa tattcccata ttggtcattt tctaataacc cgtctaccaa
    38521 atttgcaaca atatcggatg ttgctgcgcg gttttctgct agatagttca agctagatgg
    38581 atcacttgaa taaacatagt tgtaggtttt tgacgccgtg ctagaatttc cacacgcgct
    38641 caataaaact cctgtaccca ggacaagacc tgccaaggtt agatatttgc tcttagactt
    38701 tttcatttcc agaacctcct gctttaaata tttgttttat tatacaacgt ttggtttgtt
    38761 tagtcaattg tttttccaaa aaatcgaaaa aaacctcatt ttctattttt gaaagataat
    38821 tttagctatt tttacgctaa aaaaggaata agtttcaagt tctcctaaaa gattgttcaa
    38881 cttattcctt ttatttataa atgctacctt acgaaagctt aaattttgct cgcaggcgat
    38941 ctgcttgggc ttttcctatt accttatcta ataaataggt acgcagactc ataaccatat
    39001 aaagtccacc ccccatggca ccgacaagag ctacataaaa gaagctccac aaacgtccac
    39061 ttggttggaa gaaaaatcct aacagccact ggatggttcc tattaacaga aacatgacta
    39121 gggtcagcaa actgattaaa atggttcgct tcaaaatcac cttgcgcttg acaccagtta
    39181 ctttacaaat atcccgatac atcaagacgt taggaatgat gagagcaatg gttgttgaaa
    39241 tcaaaggacc ataactgtgg aagagggcga tggtaggtag ttgcaagact agcttggcaa
    39301 tagaaccata gataaaatag agaacggcct tgcggttgcg gaacatggcc tgaagcattg
    39361 gagacaagac catgtacaag cctaaaataa tagactgcaa aactgcaaag acaaataagc
    39421 ccagagccaa actatctggc ttaccataga agaccgtata aagaggttct cctaccataa
    39481 ccactccaac cgttgctggt agcaagaaca taaagagtag ggtgagactg tcctgaacga
    39541 gacgagaagc tgctttcaag tcccccttga catagttttc cgtcaaaagt ggcaaaccaa
    39601 cactcccaat cgaaacccct acagaaatca aaatcatcgt gattttatta ggattggctg
    39661 agaaataaga aaacatgaca accaagtcct cattgctgta gttggtaaac cagctcatac
    39721 tattgataaa ggtcagctga tccaaaatct ggaagagctg gatggcagac cctgtcagga
    39781 taaaaggaat ggcttcctta gtggtatcaa ccaagagacg cttactgtta atcttatctc
    39841 ctgtttcaaa gactcttttg agtgaacctt cttgggcaag gaaataaatc aagactgcaa
    39901 aactggctac cataccgaca aaggcagcaa aggttgattg ggtaacggct gctagataat
    39961 ctcctgaacc gagcttcata atgataaagg ttgctaggag catccagata acacgaatga
    40021 cctgctcagc aatttggctc atggcatagg gtttgaggtt attcatccct tggaaaaatc
    40081 ctcggataac actcatagac gggaaaatca agactcccca agccaagctt tgcatgattg
    40141 ggatcaagtc tttgcccacg ccagacaagt ctgctagcca aggagcaaag acatacaaga
    40201 ctaaagcaaa aaccaggcct agtcctgtca taaagcctaa gaagctccga atcagggcaa
    40261 agctatgctc ttcttctcgc atggtattat acttggcaac ttgcttggcc accgcaactg
    40321 gaatccccgc tgttgaaacc aacaagaacc aagcatagat attgtaaccc atggtaaaga
    40381 gaccatttgc cttagctgca taagccccca tccagatgta ccaagggata atataaacag
    40441 ccccgagtag gcgactgata aagttactag ccgttagcca agcagtcccc cgtaacatct
    40501 gggcctgctg gtgattgttt tcgtgcgaca tagattcctc attcaatttt gatagctagg
    40561 aaaaactcct tatcttccat tataaacttt tccttgttac ttgtaaattt acgactttcg
    40621 gtttacaata gaaagtatga ttaagattga aaccgtatta gatattttaa agaaagatgg
    40681 cctttttcga gaaattattg accaaggtca ttaccactac aactacagca aagttatttt
    40741 tgatagcatc agctacgaca gccgaaaagt aacagaagac actctttttt tcgcaaaagg
    40801 cgctgccttt aaaaaagaat accttctttc tgctataaca caaggattag cttggtatgt
    40861 agctgaaaag gactacgaag tcggtatccc tgtcatcatt gtgaacgata taaagaaagc
    40921 catgagtttg attgccatgg agttctatgg taatccacag gaaaaactca aactccttgc
    40981 ctttactggt actaagggta agacaacagc agcctatttc gcctataaca tcttatctca
    41041 agggcataga cctgctatgt tgtcgaccat gaacacaact cttgatggcg agactttctt
    41101 taagtcagcg ttgacaaccc ctgagagtat tgacctcttt gacatgatga atcaggctgt
    41161 gcaaaatgac cgtacccacc tcatcatgga agtctccagt caagcctatc tggtcaaacg
    41221 tgtctatggt ctaacctttg atgtgggagt tttcctaaat atcagcccag accatatcgg
    41281 cccgattgaa caccctagct ttgaagacta tttctaccac aagcgtctct tgatggaaaa
    41341 tagccgagca gtcatcatta acagtgacat ggaccacttc tcagtcttga aagaacaggt
    41401 tgaagatcaa gaccatgatt tctatggtag ccaatttgat aaccaaatcg agaattccaa
    41461 agcctttagc ttttcagcta cgggtaaact cgctggagat tatgatatcc aactcattgg
    41521 caacttcaac caagaaaatg cagttgctgc tggacttgct tgtctctgtc tcggagcaag
    41581 tcttgaggac atcaaaaaag gcatcgctgc aacccgcgtt cctggtcgta tggaagtcct
    41641 cactcagaaa aatggagcca aggtcttcat cgactatgcc cacaatgggg atagtctgaa
    41701 aaaactcatc aatgtggttg aaactcatca aaccggaaag attgctctgg ttctgggatc
    41761 aacaggaaac aagggagaaa gtcgtcgtaa ggactttggc ctcctcctca atcaacaccc
    41821 tgagattcaa gtctttctga ctgctgatga ccctaactat gaagacccaa tggccattgc
    41881 agatgaaatt agtagctaca tcaatcatcc tgttgaaaag attgcggatc gccaagaagc
    41941 catcaaggcg gcaatggcta tcacaaatca cgaattagat gcagttatta ttgcgggtaa
    42001 gggagccgat tgttaccaaa tcatccaggg caagaaagaa tcctacccag gagatacagc
    42061 cgtcgcagaa aattatttat aagaatagaa aaaatcaagg gaaaatgaaa cccttgattt
    42121 tttcctatta tttagtaaaa acgtttttca aaccttcaac ggcaccttct acagcacctt
    42181 ttgcgtcttc tacaacgtct tttgccttag caactgtttt ttcaacagct ccttcaagtt
    42241 ctgtcttgct gtctccagta actttaccaa aaccttcttt gacagcgcct gttgcttgtt
    42301 ccaatttgtt ttcaagtgac atatgattgt ctcctttagt ttattccgat atgtgttacc
    42361 ggttacatat agtttatacc gaatactaag gaaagtcaaa caatgtgctc agaaacaaga
    42421 aatcacgcta agtagaaagt taatctctcc ttatcaaaat cgatagccaa ctcatacttt
    42481 ctatcttcat tttgcacaat atagcctaag acaaccaaac tgtccacaaa gatatcacgg
    42541 catttctgca taaggtggtc ttttttgaga aatttcaaca aaaaagccgt catatacttg
    42601 agtgcatact caggattaac atctcctaaa atgtcataga gctcctgctg tttttctgtc
    42661 aaaggatact gctgtttgac cttgtaaaaa taattggaca gggtcatttt aatgcgcgca
    42721 aagtccgtct tttcaactaa aatagctgca ttgatttgat tgcgcaattc cgtctcaaaa
    42781 ctctgctcca acaaggcttg atagaccgga ctagcttctc tgacaaaaat ctcttgatcc
    42841 agttcaagac tatcaagtga ttcaagcgta ggaaaattga ggtaatagcg cttattttca
    42901 cgtagaatca agcctgcctt tatatactct tccatgagtt tatcaactgc aacatctgga
    42961 aattgagcct taatttcccg tagaatcaca tcctcatact ggtccagata gcggatcaat
    43021 tctccaaaaa tggctgtctc gtcaaacgag atggattaaa aatctgaatc atgaagattc
    43081 ctttctttac attgtaacag aggcgatgct gccaactgac taggattggt accagcttgg
    43141 ttcagaggaa caggcagacc tagttctatg taatactccc tccaaaaatc actaatctcc
    43201 tgctccccat ctccaaattt cataggaata gtttcaaaaa taggtaggat ttctgctata
    43261 tactgggagc agtagaaacc agctgcatct ggatagaaag aagcattgta gggtgctcca
    43321 agatgtttgc aagctctttc cttcaccgac tggatatcca tttctgggta aacatagagg
    43381 tcgtataaat gattggactc aaagaagtct gccggttctt gacagacaac accagcctgt
    43441 ccactagcat gataaatcat cccatccaaa taaatggcaa catggctata gttacctgtg
    43501 gaagtctgga tggcctgtcc catgtctgac ccatctctca caaaaatcaa atcgccattt
    43561 tctaacatac ttcactccta gaaaaaagga gccgaaactc ctttcgatat aaggcaaact
    43621 agactttccg attttgagct gacacccaaa atcttacgca ttaaagcttt cagtcaattg
    43681 aggtaccact tgtttcttac gtgaaacggc accagcaagg aaggcatgat tgttttcaag
    43741 tttgaagttg aaagccgctt cgaccttgtc catattggca ccaagagcca agatttctga
    43801 gtttgagttg acgatatctg taatcatcaa gacaaagtca gagtagccgt ttgattcgtt
    43861 ggcagcttgc attgcagctt caatttctgc ttggcgttcc aaaacttcag cgatgtcaac
    43921 tgtgttcact tgggcaacac ggacattatt tccgttgagt tcaaaagtct tagcatcgat
    43981 gtcaatcaat tcttcagcag atttgctagc caagttggta ccagctttca acattgccaa
    44041 accatattct tccaagttca caccagccaa ttcagccaat tcaggagcaa tgattttatc
    44101 tgttgggtgt gttgttggtg atttcaaaag aagggtatct gaaatcaaac ctgaaagcat
    44161 caaacctgca atctctttag gcacagctac accatgttct ttgaacatac ggtaaacgat
    44221 tgaagacgct gatccaactg gctccaaacg catgtaaagt gggcttgcag tttcaaagtt
    44281 agccacacgg tggtggtcta caacaccgta aacttctact tcagcgatat ctgatacaga
    44341 ttgttggaat tcattgtggt cagtcaagat aacttgctct gccccctctg ctttggcaga
    44401 agtgataaca cgtggtgctt ccacaccaaa atagttcaag acaaaggctg tttcttcatt
    44461 tggagttcca agggcaacag cttccgtatc caaaccgtaa gcttcttttg caaggtaggc
    44521 aaaagctaca gatgatccga tggcatctga gtctggattt tggtgaccaa atactagaat
    44581 cttggacatg ataatacctc attttgttta ttttctttat tgtagcattt ttttcgcttt
    44641 ttgacaaagg taagaggagt caaaggactc cccacgattc ttcaaaataa ctgagtaaat
    44701 ttctatcttg attttcagat aaaaattctt ccttctgtgg cctcttctta cgcttgagaa
    44761 gagcttcagc cgacatggct tcttccttac tggcaaaacc ttgagcatag ataagtttga
    44821 ctggcaagcg tgctcgtgta tatttggctc ccttcccact attgtggata gcgaggcgtc
    44881 ttctcatatc agtcgtatag cctatatagt aggatccatc acgacactcc agaacgtaca
    44941 tataagcctt atgatccata ataaatctct tcgatttcgg gcgtataaga gccatcatca
    45001 ttgtggacaa tcaaaggagg taagacctta aagccacttg ttgagccatc cttgatcgcc
    45061 tcaatcaaaa gcatattggc ttccttttct ctttttggat aaacaaactg caggcgctta
    45121 ggggctagat tatgtcgttt taacgtatcc aaaatatcca gaagtcgatc aggacgatga
    45181 accatggcca aacgcccatt agacttgaga atactctggg cactatgaca gatttcttcc
    45241 aaattagtcg tgatttcgtg tcgagccaag agataatgtt cactctcgtt cagattagaa
    45301 taaggatcca ccttgaaata gggtggatta cacaaaatca tatccacctt actcccctga
    45361 atgtgagcag gcatattttt caaatcatcg cagatgacct gcatttgctc ctctaatcca
    45421 ttcaaacgga cagagcgttc agccatatcc gccaaacgct cctgaatctc aacagacaat
    45481 atctgtgctt gagtacgagt gctagcaaaa agccccactg ctccattccc agcacagaaa
    45541 tccacaatca accccttctt aggaaaacgt ggaaatcgtg ataagagaac actatccacc
    45601 gaatagctaa aaacctctct attttgaatg attttgatat ctgtcgaaaa gagctggtta
    45661 atgcgctctc ctgattttaa taattgttct tcttccatgg tcctattata gcaaatttat
    45721 attaacatta caaaaaatat aaaactctaa actacttctt cttttttaaa tggtgcaggg
    45781 cttctccagt ccagattggt agcattcgtc gaaagggagc aaaaccgtag ttaaagcggt
    45841 cgcttgaaaa gcgtctccgt ctaggaaact ggtacttttc ttcctccaaa gtgcggatag
    45901 aaagactggc tttccccgta aattcatcta aatccactac ctgaacttga acctcttcat
    45961 cgactttcaa ggtttcatga atattttcaa taaatcctgt ccgaatttct gaaatgtgaa
    46021 tcagccccgt atcacccgtc tctaactcaa caaaggcacc gtagggctga atccctgtaa
    46081 tacgcccctt tagcttatca ccgattttca ccttagtcct cgatttcaat agtttcaatt
    46141 acaacatctt caactggctt gtccatagct cctgtctcaa cagcagcaat gacatccaag
    46201 acagcgtaag atgcttcatc agctaactga ccaaaaaccg tgtgacggcg gtctaggtga
    46261 ggtgtcccac cttgattggc atagatttct gcaatcggtt ctggccaacc accacgagta
    46321 atttctttct tagaataagg taggtgttgg ttttgcacga taaagaactg gctgccgttg
    46381 gtatttggac cagcatttgc catggaaaga gcaccacgga tattgtaaag ctcttctgag
    46441 aattcatcct caaaagattc gccgtagatt gactcgccac ccataccagt tccagttggg
    46501 tctccacctt ggatcataaa gtccttgata atacggtgga aaatgacacc atcatagtag
    46561 ccatcttttg aaagagatac aaagttagcc actgttttag gagcatgttc agggaaaagc
    46621 ttgatacgta agtctccgtg attggtctta atagtcgcaa gaggaccttc tactgtttca
    46681 atgtctactt gtggaaaatg caattctttt tctaccatac caaatacttc taaggcatca
    46741 aaaatgccat cttcttctaa tgtttttgta atataatctg ctttttcttt gattttatca
    46801 tgagaaattc ccatggcaac gctgattcca gcataatcaa agagttccaa gtcattgaga
    46861 ccatctccaa aaaccatgac cttctctggt ttcaagccaa ggtgttccac aaccttttcc
    46921 acccccgtcg ctttggagcc tgaaatcggc acaatatcag acgaatgttg atgccaacga
    46981 accatgcgaa gtttgtctga gagactgtca ggcaagtgca agtcatctcc cttatcttca
    47041 aaagtccaca tctgatagat atcttctttt tcatggaaat cgggatctac atctaagtcg
    47101 ggataaattg gattgatagc ttcactcatc atatcggtgc gagtcgacaa cttggcatca
    47161 tgactcccaa ccaagccata ctcaattcct tcttgcttag cccaagagat atactcctca
    47221 acatatgact tttcaatctg atgctgataa atgacctgac cttttttatc ttcgatataa
    47281 gccccattca aagttacaaa aaagtcaggc ttgagatcac gaatctctgg aacaacacca
    47341 aaaatgccac gtccagaggc gattcctgtt aaaattcctt tttcacgcaa ctgtttaaaa
    47401 acagtgggaa ttgtagttgg aataaaccct gtctttgaat tccgcaatgt atcatcaata
    47461 tcaaaaaaga caatcttgat cttctttgcc ttgtatctta atttcgcgtc catctcacta
    47521 cctctttcaa tctaactctt tccattatat cataaagtag gcaaatcccc tattttcaaa
    47581 aagtttatca tttttatttt aatttcttgg atgagaaaaa agacatattt atgaaaaagc
    47641 tccatcgtgc ttttaatgtg ttctcttgtt ttcaaactcg taaaaaggga gccactgagc
    47701 ctaactcgct ctctcatttc aaagcttgtg aaaaaagacc cgttggggtc ttaattcgct
    47761 ttcttgtttt caagctcatg aaaaagagac ccaaccgggt cttttcttta atcttcgttt
    47821 acgaaaggca tcaaagccat tacgcgagcg cgtttgatag ctgttgttac tttacgttgg
    47881 tttttagctg aagttcctgt tacacgacga ggaaggattt tcccacgttc tgaaacgaaa
    47941 cggctaagaa gctcagtatc tttgtaatca acatattcaa ttttgtttgc tgcgatgtaa
    48001 tcaacttttt tacggcgttt gaatccgcca cgacgttgtt gagccatgtt ttttctcctt
    48061 tataagttta gttgtccatt agaatggtaa atcatcatct gaaatatcca atgggtttgt
    48121 tgctccaaat ggattttcat tacgtgaaaa gtctggtact gaatttgtag gtgctgaata
    48181 gtttgcagtt ggtgcagagt aagctccacc tgtgtgaccc tcacgcacac tacggctttc
    48241 caacatttgg aaattctcag ccacgacctc tgtcacgtag acacgttgtc cttgctggtt
    48301 atcgtaacta cgagtctgga tacgacctgt caccccgata agtgagcctt ttttagccca
    48361 gttagcaaga ttttcagcct gttggcgcca cataacgaca ttaataaaat cagcctcacg
    48421 ttcaccattt tgactcttaa atgtacggtt tactgcaaga gtaaaagtcg caactgctac
    48481 atttgatggg gtataacgca actcagcgtc acgtgtcata cgccctacaa gtacaacatt
    48541 gttaatcata gtttaccttc ttacgcgtca attttgacga tcatgtgacg aagaatgtca
    48601 gcgttgattt tggaaagacg gtcaaactct ttaagagctg catcgtcatt tgcttcaacg
    48661 ttaacgatgt ggtaaagtcc ttcacggaaa tcttggattt cgtatgcaag acgacgtttt
    48721 tcccaagttt ttgattcaac aacagttgca ccgttgtcag tcaaaataga gtcaaaacgt
    48781 gctaccaaag cgtttttagc ttcttcttca atgtttggac gaatgatata aagaatttcg
    48841 tatttagcca ttgatatgtt cctccttttg gtctaatgac cccaagactt tgcaaggggt
    48901 aagtgaggtt cgctcacaat aaactattat actagaaaaa atttttttac gcaagtaaaa
    48961 acactagaat tcgaaaaaac gccacatggg cgttttcctg ttcttatggt ttgatacggt
    49021 gcaacatacg tgggaatgga atagcttcac ggatatgttt tgttcctgct gcgaaggtta
    49081 ccatacgttc gataccgata ccaaatcctc cgtgtggaac tgtaccgtat ttacgaaggt
    49141 caaggtagaa ttcatattct gtacgatcca tgtcaagttc atccatctta gcgacaaggg
    49201 catcgtaatc ttcctcacgc atagacccac cgataatttc tccatagcct tctggagcaa
    49261 gcaagtctgc acaaagcacg cgctctggat ttccaggaac tggtttcatg tagaaggcct
    49321 tgatggctgc tggatagttc atgacaaatg ttggcacacc aaagtggttt gaaatccaag
    49381 tttcgtgtgg tgacccaaag tcatcaccat gctcaagatg ctcgtagtca gcatcttcat
    49441 cattttcatg ctcttgcaag aggtcaatgg cttgatcgta agtgatacgt ttgaatggct
    49501 ctgcaatgta gcgtttcaag agttctgtat cacgttccaa ggtttccaag gcttgaggcg
    49561 cgcggtcaag aacaccttgt agaagagctt tcacataagc ttcttgcaag tcaagcgact
    49621 catcatgtgt caagtatgag tactcagcat ccatcatcca gaactcagtc aagtgacggc
    49681 gtgtttttga tttttcagca cggaaaactg gaccaaagtc aaagacacga ccaagagcca
    49741 tagcccctgc ttctaggtaa agctgacctg attggctcaa gtaggctggc gttccgaagt
    49801 agtcagtttc aaagagttct gtagaatctt ctgccgcatt tcctgaaaga attgggctgt
    49861 caaacttcat aaaaccattc ttgtcaaaga actcataagt tgcatagata atagcgttac
    49921 ggatttgcaa cacagctact tgcttacgag agcgtagcca caagtgacgg ttatccatca
    49981 aaaagtctgt tccgtgttct tttggtgtga ttgggtagtc ttgagattca ccgatcactt
    50041 cgatgtctgt gatgtccaac tcatagccaa atttagaacg ttcgtcctct ttgacaatac
    50101 ctgtcacata aacagacgtt tcttggctca agcgtttgat aacatcaaac ttctcaagtc
    50161 ccacttcttc accaaatttt tcgacaaagt ttggtttaaa agccacacct tgaaagaagg
    50221 ctgttccatc acgcaattgt aagaaagcga ttttcccttt tcctgatttg ttggcaaccc
    50281 aagcgccaat cgtcacttcc tgaccaacat agtcttttac atcaataatc gttacacgtt
    50341 ttgtcattat ttttcctttt cttttttatt ctttatggca aaccacctct atattgttcc
    50401 catccaggtc aatcataaaa gcagcataat aaatcggatg ctcacttcga taaccaggaa
    50461 ccccattgtc tcgcccacct gcctctaagc cagcctcata acaagcctga acttcttcct
    50521 tattttctgc taaaaaagca aaatgaacag gatcttgtgt tccctgagtc agccaaaaat
    50581 caccaccagg atgagggctg ttcggggata gaaaactaat tagagaacta gtcttaaaag
    50641 tcaatttata gtccaaagga gcgagaaaac tcctataaaa tccttctgaa atttgtaaat
    50701 cctttacctt aatctcaaaa tgatcaatca ttctcactac ccataaatgc tttcaagcgt
    50761 tcgactgctt ctttaagcgt gtctaggtct gtcgcatagc tgaggcggac attttctggt
    50821 gctccaaatc cagctcctgt taccaaggcc acttcggctt cttctaagat agcagttgta
    50881 aagtctgtca catccgtgta gcctttcatc tccatggcct ttttgacatt tgggaagaga
    50941 tagaaggccc cttgtggttt gaccacttca aatcctggca cctctgcaag gaggggatag
    51001 atggtattaa gacgttcctc aaaggcctga cgcatgcttt ctacagtatc ttgctcacct
    51061 gatagagcct caactgctgc atattgggct actgctgacg gattcgaagt tgtttgacct
    51121 gcaatcttgg acatggcagc gataatgtct gcttctccaa cggcataacc aatccgccaa
    51181 ccagtcatgg cataggtttt agacacacca ttgatgacca ctgtttgctt gcgaatcgct
    51241 tccgataggc tagaaatcgg tgtgaactca tgaccattat aaaccaagcg gccatagata
    51301 tcatctgcta ggatgagaat atcattttct acagcccagt ttccaattgc caagagttcc
    51361 tcacgggtgt aaatcatacc tgtgggatta gatggcgaat tcagcaccaa aaccttggtc
    51421 ttgtcagtgc gagctgcttc taactgctct acggtcacct taaagtgatt gtcttcctta
    51481 gcagaaacaa agacgggaac gccttctgcc atcttgacct gatctccata gctaacccag
    51541 tatggggttg ggatgatgac ttcatcacct ggattgacca cagccataaa gaaggtatag
    51601 agagaatatt tggctcccgc agcgactgtc acttgatttg acgctacaga atagccgtaa
    51661 aagcgctcaa agtagctatt gaccaccgcc ttaagctctg gcagacctga ggttactgta
    51721 taaaaagaag cacgcccatc tcgaatcgat gcaatggcgg catcttggat atttttggga
    51781 gtagtgaaat ctggctcacc caaggttaga gacaaaatat ctctaccctc agccttcagt
    51841 gctttggcac gggctccagt agccaaagtc acactttctt ccatttctaa aacacggttg
    51901 gatagtttca taggccctcc ttattgacca atgctcctgt ttcaaaatct actagataaa
    51961 aatcagatcc tgacttaact tcccagattg gcttgtcttg ataacgacca aaggttatct
    52021 tgtcaatctc gccagctccc ttttccttag aaaccgtttc tgctttttct tgtgaaatac
    52081 cctgatttag ctgataaacg taaatcttat ggtcatcttt accaatcagg acagcaagcg
    52141 cttcttgctg tttattacga ccaagaacgc tgtaataaga ttccaagcca ttgtataaat
    52201 caacctgatc agcctgctct aatcctgcat actgctgagc taatttttct ccttcacttt
    52261 tagctgtttg atagggtttc atgctaagag aaaccatata cagaaaggaa gcactgataa
    52321 ccacaaacaa aatcgtcatc cctagaccat actgccacag tagattattt tttgctttgt
    52381 tttgtctttt tttcactcgt ctattttacc atctattaag ctttattaca agtgaatata
    52441 agaatactct tcgaaaatct cttcaaacca cgtcagcttt atctgcaacc tcaaagctgt
    52501 gctttgagca accaattcta tttctccctt caaacaaaac cgattttgaa agtgaaacag
    52561 ttcttacttt ttcagtcaca aatgactaga gtttccgaaa ttttacaatt ttcaaataaa
    52621 ctctgagcaa atttcttgca agttcttttg ttttgttgta atatatttta taacaacgag
    52681 agagttctcg aaattttaag aaaaaggaga cacatcatgt ctaaaaaagt attatttatc
    52741 gtcggatcac tacgtcaagg ttctttcaac caccaaatgg cgctcgaagc tgagaaagca
    52801 cttgctggta aagcggaagt tagctacctt gattattcag cccttcctct cttcagccaa
    52861 gatttggaag ttccaacaca tccagctgta gctgctgctc gtgaagcagt tctcgttgcg
    52921 gatgctatct ggattttctc tccagtctac aacttctcta tccctggtac agtgaaaaac
    52981 ttgcttgact ggctatctcg tgcccttgac ttgtctgata cacgtggcgt ttctgccctt
    53041 caagacaagt ttgtcacagt atcatctgta gccaatgcag ggcacgatca acttttcgct
    53101 atctacaaag acctcttgcc atttatccgt acacaaggcg ttggtgattt cactgctgca
    53161 cgtgttaatg actctgcctg ggcagacgga aaattggttc ttgaagaaac agtcctaaac
    53221 tcacttgaaa aacaagctca agacttggtc gaagctatca agtaactaac actcaataaa
    53281 aatcaaaaag caaactagga agctagccgc aagctgctca aagcactgct ttgaggttgt
    53341 agatagaact gacgaagtca gtgacatata tacggtaagg cgacgctgac gtggcttgaa
    53401 gagattttcg aagagtataa cataataaaa gcgaacaaga ctacttttct gacactcaga
    53461 agactagctt gttcgctttt tttctcattt ataaactaaa acagctgatg atacatattg
    53521 ccaaatacaa aatatctgat ttctatactt cctttacaaa aaccaatccc tgtggttgtg
    53581 acaaatcttc tcggtaaaca aatccagcaa agttcaagcg acgtgcttcc cccacagttt
    53641 gtacttgatt ttctattaaa gctgttagaa aggccccgcg cgctttcttg gagatagttg
    53701 agtgaatctt cagctgaccg cctctatcct ccatgaattt gaaggtcacc atctttgctc
    53761 tgatttcctt agaaaataca gtctcaaact ctgatgacaa gagagagaaa atcacttctt
    53821 ccttcttcag agcttcatca tagactgcct tccaatggct cttcaaagtc ttaccagcga
    53881 cttttaattt catcaaaaaa tccaaacggt gaggagccat gggtgacaag gctggaacaa
    53941 caccgtacaa agccgaggta atgaagacat gattttcaag ataatcttgt tccgcctcgg
    54001 tcagcttatc tctcttaatg ttgcggtaca taagcccatc aaaaagtttc aaggctggat
    54061 agtgttgagc agtttgcctt ttcaaagctt ggatattttg aaattcttcc gccgctttct
    54121 cagccgatac cttgtagaaa ctctccaatt gactggcaga atagagagcc aaggcatcaa
    54181 gcacggtctg actttctggt tttaaaggaa ttgcctcgat acttggcaag tctgtgttca
    54241 tttcttttgc tgttgggatt aaaattttca tattgatagt gtagcatatt ttttagccac
    54301 aaccaagaaa ttcatttgaa aaacctcggt ttgaccgagg tttttctatt tatttgggcc
    54361 atcctaacca gacagccacg agaacaaggg ctgtgccagc taaacttgtt aagatagata
    54421 agaatttatc ttttttctcc ttgaactgag aaaaacctat cttcaaagcg agcagtccga
    54481 gtagcattga agcaaccatc aaataaaaac ccatttcttt gtcctccatt aggcttaatt
    54541 taccttatta taacatatat ttattaaata aacctt
//
LOCUS       DAVKAO010000006        49794 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKAO010000006 DAVKAO010000000
VERSION     DAVKAO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.006"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            436..630
                     /locus_tag="VWX16_000381"
     CDS             436..630
                     /locus_tag="VWX16_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157794.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIHGALGFGFM
                     IFVVFPMEMEWLKGESHDGG"
     gene            617..1501
                     /locus_tag="VWX16_000382"
     CDS             617..1501
                     /locus_tag="VWX16_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157795.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELITFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            1503..2240
                     /locus_tag="VWX16_000383"
     CDS             1503..2240
                     /locus_tag="VWX16_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9157796.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GEPANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            2240..3337
                     /locus_tag="VWX16_000384"
     CDS             2240..3337
                     /locus_tag="VWX16_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9157797.1"
                     /translation="MLERLKSIHYMFWASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VEQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMCEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     DSMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            3339..3938
                     /locus_tag="VWX16_000385"
     CDS             3339..3938
                     /locus_tag="VWX16_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9157798.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYFSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            4363..5373
                     /gene="ccpA"
                     /locus_tag="VWX16_000386"
     CDS             4363..5373
                     /gene="ccpA"
                     /locus_tag="VWX16_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9157799.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYDLAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            5437..5808
                     /locus_tag="VWX16_000387"
     CDS             5437..5808
                     /locus_tag="VWX16_000387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157800.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADETDEVS
                     SKHVFEVVDETDEVSSKHVFEVVDETDEVSSKHVFEVVDETDEVSSKHVFEVVDETDE
                     VSNHTYGKATLTWFAEIFEEY"
     gene            complement(5805..6767)
                     /locus_tag="VWX16_000388"
     CDS             complement(5805..6767)
                     /locus_tag="VWX16_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9157801.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            6833..8221
                     /locus_tag="VWX16_000389"
     CDS             6833..8221
                     /locus_tag="VWX16_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9157802.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            complement(8256..8708)
                     /locus_tag="VWX16_000390"
     CDS             complement(8256..8708)
                     /locus_tag="VWX16_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9157803.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIVHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            8870..9163
                     /locus_tag="VWX16_000391"
     CDS             8870..9163
                     /locus_tag="VWX16_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157804.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            9319..10533
                     /locus_tag="VWX16_000392"
     CDS             9319..10533
                     /locus_tag="VWX16_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9157805.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            10678..10812
                     /gene="rpmH"
                     /locus_tag="VWX16_000393"
     CDS             10678..10812
                     /gene="rpmH"
                     /locus_tag="VWX16_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9157806.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAV
                     "
     gene            11075..11728
                     /locus_tag="VWX16_000394"
     CDS             11075..11728
                     /locus_tag="VWX16_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9157807.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKPETTPGGDKGGNTGNSD
                     ANANAGGGSQAGGPAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKDKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRPVAPKTT
                     SAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTRRRK"
     gene            12034..12807
                     /locus_tag="VWX16_000395"
     CDS             12034..12807
                     /locus_tag="VWX16_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9157808.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            12807..13367
                     /gene="rnmV"
                     /locus_tag="VWX16_000396"
     CDS             12807..13367
                     /gene="rnmV"
                     /locus_tag="VWX16_000396"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9157809.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            13439..14338
                     /locus_tag="VWX16_000397"
     CDS             13439..14338
                     /locus_tag="VWX16_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9157810.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQGLTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            14658..14954
                     /locus_tag="VWX16_000398"
     CDS             14658..14954
                     /locus_tag="VWX16_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9157811.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            15031..17061
                     /locus_tag="VWX16_000399"
     CDS             15031..17061
                     /locus_tag="VWX16_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9157812.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GARPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRDLMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            17063..17704
                     /locus_tag="VWX16_000400"
     CDS             17063..17704
                     /locus_tag="VWX16_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157813.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            17697..18005
                     /locus_tag="VWX16_000401"
     CDS             17697..18005
                     /locus_tag="VWX16_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157814.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            18136..19008
                     /gene="rsmA"
                     /locus_tag="VWX16_000402"
     CDS             18136..19008
                     /gene="rsmA"
                     /locus_tag="VWX16_000402"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9157815.1"
                     /translation="MRIADYSVTKAVLERYGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAECAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            19010..19888
                     /gene="rsgA"
                     /locus_tag="VWX16_000403"
     CDS             19010..19888
                     /gene="rsgA"
                     /locus_tag="VWX16_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9157816.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            19899..20555
                     /locus_tag="VWX16_000404"
     CDS             19899..20555
                     /locus_tag="VWX16_000404"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9157817.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            20518..21180
                     /locus_tag="VWX16_000405"
     CDS             20518..21180
                     /locus_tag="VWX16_000405"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9157818.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLQPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            21182..22438
                     /locus_tag="VWX16_000406"
     CDS             21182..22438
                     /locus_tag="VWX16_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9157819.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            22428..23369
                     /locus_tag="VWX16_000407"
     CDS             22428..23369
                     /locus_tag="VWX16_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9157820.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            23636..24463
                     /gene="purR"
                     /locus_tag="VWX16_000408"
     CDS             23636..24463
                     /gene="purR"
                     /locus_tag="VWX16_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9157821.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            24530..25780
                     /locus_tag="VWX16_000409"
     CDS             24530..25780
                     /locus_tag="VWX16_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9157822.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQESNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGE
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            25906..26700
                     /gene="pflA"
                     /locus_tag="VWX16_000410"
     CDS             25906..26700
                     /gene="pflA"
                     /locus_tag="VWX16_000410"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9157823.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(26775..27701)
                     /gene="yidC"
                     /locus_tag="VWX16_000411"
     CDS             complement(26775..27701)
                     /gene="yidC"
                     /locus_tag="VWX16_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9157824.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(27779..28057)
                     /locus_tag="VWX16_000412"
     CDS             complement(27779..28057)
                     /locus_tag="VWX16_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9157825.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            28091..28831
                     /locus_tag="VWX16_000413"
     CDS             28091..28831
                     /locus_tag="VWX16_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9157826.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVATGILIFHLS"
     gene            28887..29570
                     /locus_tag="VWX16_000414"
     CDS             28887..29570
                     /locus_tag="VWX16_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9157827.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            29682..30674
                     /locus_tag="VWX16_000415"
     CDS             29682..30674
                     /locus_tag="VWX16_000415"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9157828.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            30739..31278
                     /gene="rsmD"
                     /locus_tag="VWX16_000416"
     CDS             30739..31278
                     /gene="rsmD"
                     /locus_tag="VWX16_000416"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9157829.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSNAVLVERGRKAQTIVAENIQMTKEIGKFQLLKMDAER
                     ALERVSGEFDLVFLDPPYTKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            31268..31756
                     /gene="coaD"
                     /locus_tag="VWX16_000417"
     CDS             31268..31756
                     /gene="coaD"
                     /locus_tag="VWX16_000417"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9157830.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPIENRKRGLEKAVKHLGNVKVVSSHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSPDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            31740..32777
                     /locus_tag="VWX16_000418"
     CDS             31740..32777
                     /locus_tag="VWX16_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9157831.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDVKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            32835..33065
                     /locus_tag="VWX16_000419"
     CDS             32835..33065
                     /locus_tag="VWX16_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9157832.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDENTRKVRS
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            33089..34372
                     /gene="murA"
                     /locus_tag="VWX16_000420"
     CDS             33089..34372
                     /gene="murA"
                     /locus_tag="VWX16_000420"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9157833.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEETHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            34365..34553
                     /locus_tag="VWX16_000421"
     CDS             34365..34553
                     /locus_tag="VWX16_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9157834.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            34592..35416
                     /gene="endA"
                     /locus_tag="VWX16_000422"
     CDS             34592..35416
                     /gene="endA"
                     /locus_tag="VWX16_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9157835.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            35707..37038
                     /locus_tag="VWX16_000423"
     CDS             35707..37038
                     /locus_tag="VWX16_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9157836.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(37236..37306)
                     /locus_tag="VWX16_000424"
     tRNA            complement(37236..37306)
                     /locus_tag="VWX16_000424"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37272..37274),aa:Cys,seq:gca)
     gene            37722..41333
                     /gene="rpoB"
                     /locus_tag="VWX16_000425"
     CDS             37722..41333
                     /gene="rpoB"
                     /locus_tag="VWX16_000425"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9157837.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            41366..45043
                     /gene="rpoC"
                     /locus_tag="VWX16_000426"
     CDS             41366..45043
                     /gene="rpoC"
                     /locus_tag="VWX16_000426"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9157838.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            45154..45567
                     /gene="ndk"
                     /locus_tag="VWX16_000427"
     CDS             45154..45567
                     /gene="ndk"
                     /locus_tag="VWX16_000427"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9157839.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(46496..47128)
                     /locus_tag="VWX16_000428"
     CDS             complement(46496..47128)
                     /locus_tag="VWX16_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9157840.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(47130..49145)
                     /locus_tag="VWX16_000429"
     CDS             complement(47130..49145)
                     /locus_tag="VWX16_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9157841.1"
                     /translation="MKKLFILISNLLASLFFVWVLTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKAIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGEGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEIWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRISSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
BASE COUNT        14603 a         9506 c        11344 g        14341 t
ORIGIN      
        1 ctgataaaaa tttctactct ttttgatttt aaataagtat tagtttacat tatggtgtga
       61 attgagtttg atatctcttt tgaggaagtt gccttagatt tttctgattg tgttttattg
      121 tataatgtat cttgcttgtt ttgaacagaa ttttttatga catttgtcat attttctagt
      181 gacagaagct tctgcctcct ctgattttaa aagactataa ttgtagtatg aaatggggga
      241 agaagagatg agaaataaaa tgattattgc aatgagttta gtagtaacag gagttatgac
      301 ctatctcatg ttttcgggat tggatgagga tttctgccat tttccttgga aggtctttgc
      361 tggctttgga atcatgtctt gacttgttag agaaggtttg aaattagtca gagatgtgaa
      421 aaaggagttt gaagaatgaa gagagggatc atctatttct ttatcggcct gtcactcttg
      481 gtatggttag tggaaatgtt tactgattgg tttgatcaag ccttgcttcg ccaattcatt
      541 catggtgctt tggggtttgg atttatgatt ttcgtcgttt tccctatgga aatggagtgg
      601 ttgaaaggag agtctcatga cggtggttaa agttgaaaaa ttgagtaaga aaataaaaga
      661 caaggaaatt ttgcggaaca tctcctttga aatcaacgat ggtgaatgtg ttgccttaat
      721 tggtccaaat ggtgccggaa agacaacact cttagattgt ttgcttggag ataaactggt
      781 cacaagcggt caagtatcta ttcaaggttt accagtgacg agttctaagt tagactatac
      841 tagggcttac ctccctcaag aaaatgtaat cgttcagaaa ttaaaagtta aagagttgat
      901 tactttcttt caacgtatct atccaaatcc tttgagcaat caggaaattg atcaactatt
      961 gcagtttgtc aagcaacaaa aagaacagtt ggcagaaaaa ttatcaggcg gtcaaaagcg
     1021 tctcttttct ttcgtcttga ccttaattgg gcgaccaaag attgtttttt tagatgagcc
     1081 tactgcgtcc atggatacct caactcgtca acgtttttgg gaaattgtcc aggagttaaa
     1141 agcgcaagga gtcaccattc tctattcgtc ccattatatt gaggaagtgg aacacactgc
     1201 agaccgcatt ttgctcttaa ataagggaga gttgattcga gatacgacgc ctctagctat
     1261 gcgtagcgag gaaatagaaa agcactttat ccttcctata gcttacaagg aagtcgtaga
     1321 gcagtcaaat ttggttgaaa actggaccct aaagcaagat tctttacaag tagtcactcg
     1381 agaagcagat gctttctggg aactattagc tcaagcagga tgtaggatgc aagaaatcga
     1441 agttaataat cgtagtttgt tgaatacaat ctttgaagag acgcagaagg gagataacta
     1501 agatgaaacg atggatagca cttagtaaga tagaattttt attgaccaaa cgacaattaa
     1561 tctattatct attgtcggta gggatgccga cggctttcta tttatttttt tctggtatct
     1621 atcaggacac accaggtgaa cccgcgaatt ttatgcgtga ttacctcatc tccatgactg
     1681 ccttttccat gatgtcgaca actatcctct catttccagc tgttttacat accgataaga
     1741 tgaataactg gcagaaaaca ttgcgtcata gccctgtaaa tatggtagaa tattatctat
     1801 caaagataac aagtatgctg gttgattatt tggtctcaat tctggttgtt tttacagttg
     1861 gacattttgt aagaggtgta gacatgcctc tagtaaaatg ggttggagct gcgattttgc
     1921 tgattgtggg gagtattgct tttgtagcac ttggcttgac cttgactctc ttaccaacta
     1981 gtcagctgat gtctgttgtg ggcaatcttc tttatctagg attggctgtt ttaggcggac
     2041 tctggatgcc catttcttta tttccagact ggatgcaagc agttggtaag tgtctcccga
     2101 cctatcaatt gatggagttg ctcaagacct tcttaaacga gggtggcatc aatctatcag
     2161 ccacagttta tttacttgtt ttttcagcag ttttatttgg tttgactatt taccttcaag
     2221 gtcataagga gaatgcttaa tgcttgaaag actgaaaagc atacactata tgttttgggc
     2281 cagtttgatt tttatgcttt ttcccatcct acctgtagtg attggggagc ttcctgcttg
     2341 gcatttattg gttgatattc tatttgtagt gacgtatttg ggtgttttaa taactaagaa
     2401 ccagcgccta tcttggcttt tctgggggct catgctggtc tatgtagctg ggaataccgc
     2461 ctttgttgct gggaattata tctggttttt ctttttcctt tccaatctct taatttatca
     2521 ttttggcgta cgtagtttaa agtctttaca tgtatggaca tttctccttg ctcaagtcct
     2581 tgttgtgggg cgacttttga tttttcagag aatcgaagtt gagtttctag tctatatgct
     2641 tgtaattctt acttttgtcg atttgatgac tttgggctcg gttcgaattc gccttgtcga
     2701 ggatttgaaa gaagctcagg tcgagcaaaa tacccagata aatctattgc tagcagaaaa
     2761 tgagcgcaat cgtatcggtc aggatttgca tgatagtctg ggacatacct ttgctatgct
     2821 tagtgtcaag acagatctag ccttgcagct atttcaaatg caggcttatc cacaagtgga
     2881 aaaggaatta agagaaatac agcaaattag caaagaatca atgtgtgaag ttcgaaccat
     2941 tgtggaaaat cttaagtcta gaactttgac atccgaacta gagactgtga aaaagatgtt
     3001 agaaattgct ggaattgagg tggaaacaga taaccaacta gatactgcta gccttactca
     3061 agaattggat tcaatggctt ctatgatttt gcttgagttg gtgaccaata tcatcaaaca
     3121 tgccaaagcg tctaaagctt acttaaaatt agaacggaca gagaaggaac tcattttaac
     3181 agtaagtgat gatggctgcg gctttgcttt tctaaaagga gatgagctcc atacagtccg
     3241 agatcgtgtt tttccatttt caggagaagt aagtgtaatc agtcagaaac atccaacgga
     3301 agtgcaagtt cgactacctt ataaggagag aaactaagat gaaagtatta gtcgcagaag
     3361 atcaaagtat gttgcgagat gccatgtgtc aattgctcac gcttcaacca gatgtggagt
     3421 ctgtccttca agccaagaat gggcaagaag caatccaact attagaaaag gagtctgtag
     3481 atatcgccat ccttgacgta gaaatgcctg ttaagacagg tcttgaagtc ttggagtgga
     3541 tacgatcaga aaggcttgaa acaaaggtgg ttgtggtgac gaccttcaag cgtgctgggt
     3601 attttgaacg tgcggtcaag gctggagtgg atgcttatgt attaaaggaa cgaagcattg
     3661 cagacctcat gcaaaccttg cacaccgtcc tcgaaggacg caaggagtat tcgcctgaat
     3721 tgatggaagt ggtgatgacc cgtcccaatc cgttgacaga acaagagatt gctgtcttaa
     3781 agggaatcgc ccggggctta tccaaccaag aaatcgcaga tcagctttac ttctcaaacg
     3841 gaactattcg aaactatgtc accaatattc tttcaaaact ggatgctggt aatcgaacag
     3901 aggcagctaa tatcgcaaaa gaatctggtt ggttatgatg atataatatt ttcatgaaga
     3961 ctatgtgcta aaattgacgg tagtgaatgg aacaatacct atagtagatt gaaactagaa
     4021 tagtacacct ctacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgattta
     4081 tcctgttctt atttcatttt actataactt taggagggct tagtttcctc ctttttgttt
     4141 agtctaaggt ggtagcaagg agctaaacct aggttttcta tgaaattttt tcaatcaatc
     4201 ttcaaaaaac acttgaaagt gtttacaatt agtgatagaa tggaataagt agaaatatga
     4261 aaacgaaatt ttcacatctt cacttttgta agtagatata gagcttcaat cagcacctct
     4321 acttgtaaaa gaatatgtga gatagaaagg acaggaaaag gaatgaatgc agatgataca
     4381 gtaaccattt atgatgtcgc tcgtgaagca ggtgtttcca tggcgacggt cagccgtgtg
     4441 gtcaatggca ataaaaatgt aaaagagaat acccgtaaaa aagtgctaga ggtaattgat
     4501 cgtttggatt atcgtccaaa tgcagttgcg cgtggtcttg caagtaaaaa gacaaccact
     4561 gtcggtgtcg tgattccaaa tattaccaat ggttattttt cgagtttggc taaggggatt
     4621 gatgatattg cagaaatgta caagtacaat attgtcctag ctaatagcga tgaagataac
     4681 gagaaagaag tttctgttgt caataccctc ttttcaaagc aggtagatgg cattatctat
     4741 atgggttatc acttgacaga taaaattcgc tcagaatttt cgcgctcacg tactccgatt
     4801 gttctcgcag gaactgtcga tgttgagcac cagttgccaa gtgtcaatat tgactataag
     4861 caagcaacaa ttgatgcagt gagttacctt gctaaagaaa atgagcgtat tgctttcgtt
     4921 agcggtccgc tagtggatga catcaatggt aaggttcgtt tagttggcta caaggaaacc
     4981 ttgaaaaaag caggaatcac ttatagtgag ggtttggtat ttgaatctaa atatagctat
     5041 gatgatggtt acgacttagc agagcgtttg atttcatcaa atgcaactgc agcagttgtg
     5101 acaggtgatg agttggcagc aggagtcttg aacggtttgg ctgataaagg tgtttctgtg
     5161 ccagaagatt ttgaaattat tactagtgat gattcacaaa tctcacgctt tacccgtcca
     5221 aacttgacaa cgattgccca acctctttat gaccttggtg ctattagtat gcgtatgttg
     5281 accaagatta tgcataagga agagttggaa gaacgtgaag ttctcttacc tcatggtttg
     5341 acagaacgta gctcaacacg aaaacgtaaa tagaaaaaat cagggaatcg agaagatttc
     5401 ctgattttgt gctcttaaac aagagatgtt gacttaatga ataccataga gcggactagg
     5461 cggctggtta aaggctgcgc aacacactgt tttgaggttg tagatagaac tgacgaagtc
     5521 agctcaaagc actgctttga ggttgcagat gaaactgacg aagtcagctc aaaacatgtt
     5581 tttgaggttg tggatgaaac tgacgaagtc agctcaaaac atgtttttga ggttgtggat
     5641 gaaactgacg aagtcagctc aaaacatgtt tttgaggttg tagatgaaac tgacgaagtc
     5701 agctcaaaac atgtttttga ggttgtagat gaaactgacg aagtcagtaa ccatacatac
     5761 ggtaaggcga cgctgacgtg gtttgcagag attttcgaag agtattagcc ttccatatag
     5821 tctttcaaag cctgtcctgt tagtccggca ttgagggcga tgaggagttt caagcgagct
     5881 ttttgggcgt tgagttcttt aacaaagaaa acgcctgctt tttgcaactg tacgccccca
     5941 ccctggtagg cataaacagg ctcggcaata ccgttaaagc atcgtgatac cagagcgact
     6001 gggattcctt tttgcagaag gctttctaat ttttgagccg tttctttggg aatattacca
     6061 gctccgaagg cttggataat caaaccgtcc aagtgttcta aatccagcat atcaatcagc
     6121 tcatctgtca taccagcata agccgagatg atagggacta aaccttgtat gtgatcaagg
     6181 tcaaagcgaa cacgaggttc agctgttttg aagtagagga tttcctgttt catgatgaga
     6241 ccgagggggc catgtgttgg agtctggaag gtgctgacat tagtcgtatg tgttttggtg
     6301 acatacttgg cagcgtggat ttcatcgttc ataacgacca aaactccttt gtcagcagcc
     6361 ctgtcatcgc tggccactcg taaagcactt aggtaattat aaacaccatc actaccgagc
     6421 tcattggagc tacgcatggc tcctgttaga acgataggca tatggggaac ttccatggta
     6481 tcaaggaaat aggctgtttc ctctaaagta tcggttccgt gtgtgatcac cactccatcg
     6541 tagttatctg cttcctcttt aattttctgg tagaggacca gcatatgttt gggtttgata
     6601 tggggacttg gaaggttaaa aaagtccaag gcgtggactt ggattccttc aagtgggttg
     6661 gacacatggt tcatgggatt atctgaactc gtcacaacag caccagaagc atcggcctgc
     6721 atggaaatag ttccacccgt atgtaaaaca aggattttct taggcatgat ttactaactc
     6781 tttctgaaat gtggtataat cattgtatca caaaagggga gattggaata ggatggaagt
     6841 caaagctgtt ttttttgata tcgatggaac cttggtcaac aatcgcaaga gtgttttgaa
     6901 atccactaag gacgcgatta agattgtcaa agaacaaggg gtactagttg gcgtagcgac
     6961 agggcgagga cctttttttg ttaaggaatt gatggacgat ttggatctgg attttgcggt
     7021 aacctataat ggccagtata tctttagtaa agacagagtc ttgttcacga gccctatttc
     7081 caagttacat ttgcgccatc taattagcta tgctaaaaaa gagggcacgg agattgctct
     7141 agggaccaag gatgccatgt tgggttctaa aatcatgtcc tttggtctgg gttctttttc
     7201 ccaacgaatt agtcgcttcg ttccctctgt tttaactcgg acggtgagtc agtcttttaa
     7261 tcgcatggtc agcaaggttg ttccccaaaa ggaagaggac ctgcttcacc tgatgaatca
     7321 gcctatctac caagttttga tgctgatgac accagaagaa tctgagaagg cggcagctga
     7381 ttttgaagac ttgaaattga cacgtagcaa tccttttgca tcggatgtca tcaatcaggg
     7441 aaattctaaa ttagaaggca ttcgccgagt tgggaaagaa tatggctttg acctcaacca
     7501 agtcatggct tttggtgact cagataatga ccttgaaatg ctggctggcg tcggtatgtc
     7561 ggtcgctatg ggaaatggta gtagcagtgt caaggaagct gccaagcaca ttaccactag
     7621 caatcaacaa gatgggatcc acaaggcctt ggaacatttt ggtgttttgt cttcagaaaa
     7681 agtctttgtc agccgtgact atcatttcaa taaggtcaag accttccacc acatgatgga
     7741 tgaacgaacc caagaagaac ctcgagcttg ggatttagag ggtgcaaccc atagggctgg
     7801 ttttaaaata gaagaattgg tggagtttgt tcgagcagcc agtccttctg aagaagattt
     7861 tggtcaagct gtatcgcaac ttcatcaggc ccttgataag gcagcaggta aagtagccaa
     7921 gaagacacct gctcagcaag atttgatagg gcaagtggat gccttgattg acacgcttta
     7981 cttcacctac ggcagttttg tcttgatggg ggtggaccca gaacgtattt ttgatattgt
     8041 ccatcaggcc aatatgggga aaatttttcc agatggcaag gctcattttg atccagtgac
     8101 ccataaaatc ttaaaaccag ataattggga agaaaaatat gctccagaac ctgctattaa
     8161 aaaggaactg cagcgccagc tcaaggctta cgaacgccat aaagagagaa ataaatcgta
     8221 aacaaaaagg agctaggggc tccttttctt tgtgattata aggttttttc ttgttctctc
     8281 acaaccagca aatcgacctt agcatggcgg agtatgtatt cagatgaaga gccgactaag
     8341 aggcgttcaa aggcgttgag accagttgcg ccaacgagga tgaggtccac ttcctcggca
     8401 tctggaatag tacgtgctag cagggtcttt ggatttccca tttcaatgac gatatgaaca
     8461 tctgctaccc cagcatcttt agcacgtttt tcgtactctt tcatcagact ttcagcgtcg
     8521 acttggagtt cttcgtaaac ttcagcatca aaggtggata cgctttggag agcgcgtgtg
     8581 tcaatgacat gtacgatggt gagtttagcg tcgtttcgta gagcagaatg aactcccttg
     8641 acaaaagcca agtccgcttc cttagaacca tcgattgcga ccatgatatt ttggtaacgt
     8701 tgtgccataa tgaaacctcc tgaaattttc ttactataat tgtaaacctt ttcactatag
     8761 aagtaaagca aaaaaagtat ttttcaaaag aatattaggg cttaaactct atataaatat
     8821 atgataaaat aaaagaaagg atagcaggaa agaagtgaag gaagggagaa tgatgaaaaa
     8881 ctcttttcaa aaatcaaatt tcctttatta tggaattatc ctagtttcag tcttggtaga
     8941 agtgatcatg atctgtcaat tagagagtct ggtaccgctt ctttatccaa gttttattgg
     9001 atttttagtt tttcatgtac tctaccatct aatcttattt ctagttgcaa aacgaagtgg
     9061 gcgttgggat tacttgatga tatgggggct tttcctcatg tttaatcttc tttatgactc
     9121 ctttctaggc ttgctttttc taggtttatc ttttggtatg tgatgtgtct ttgcaaaata
     9181 cctgctatct ctacgttctt aacctcgcct ttagcaaggt ttttcttcta tacgaaacta
     9241 gtaacacatt tcagtaaact tgtcgcattc tctttctagt ggtataatgt tactatcctg
     9301 atgaattgag gaaacaagat gaaagaatat aacaagtcta gtaagttaga gcatgttgct
     9361 tatgatatcc gtggtcctgt attggaagaa gccatgcgga tgcgagcgaa tggagaaaag
     9421 attttacgtc tgaatacagg aaatccagca gaatttggct ttacagcgcc agacgaggtc
     9481 attcatgact tgattatgaa tgcgcgtgat agtgaaggct attctgactc caaaggaatt
     9541 ttctcagccc gtaaggccat catgcagtat tgccagctga agaaattccc aaacgtagat
     9601 attgatgata tttaccttgg aaatggtgtc agtgagttga ttgtcatgtc catgcagggg
     9661 cttttggaca atggggatga ggtcttagtg ccaatgccag actatcctct atggacggcg
     9721 gctgtcagcc tagctggagg aaatgccgtt cactatatct gtgatgaagc tgttgaatgg
     9781 tacccagata ttgacgatat caagtcaaaa atcacttcca ataccaaggc aatcgtcctt
     9841 atcaatccaa ataacccaac tggagccctt tatcctaagg aactcttgct ggagattatt
     9901 gagattgccc gtcaaaacga tttgattatc tttgcggatg aaatctatga ccgcatggta
     9961 atggacggac atgtgcatac gcctgtggcg agcttggcac cagatgtctt ctgtgtcagc
    10021 atgaatggtc tgtcaaaatc ccaccgcata gcaggtttcc gtgtgggttg gatggtcttg
    10081 tctggcccta agactcatgt taagggctat atcgaagggc tcaatatgct gtccaatatg
    10141 cgcctttgct ctaacgtttt ggcccaacaa gtcgtacaaa cttccttggg gggtcaccaa
    10201 tcagtcgatg aattgcttct tcctggtgga cgaatctacg agcaaagaaa tttcatctat
    10261 aatgccattc aagatattcc aggtttgtct gccgttaaac ccaaggcggg gctctatatc
    10321 ttcccaaaaa tcgaccgcaa tatgtaccgt atcgatgatg atgagcagtt tgtccttgat
    10381 ttcttgaagc aggaaaaggt tctcttggtt catggtcgag gctttaactg gcaggaacca
    10441 gaccacttcc gtatcgttta ccttcctcgt gttgatgagt tagcccaaat ccaagaaaag
    10501 atgactcgtt tcttgaaaca gtatcgtaga tagggcttgc attcgaaaaa gctggaaaca
    10561 tttgcctaga gctattgaca aaagtgaaag aatcagatag aatagtaaag tatgtttagt
    10621 aactgatcac tttatagccg gctaaacgaa tacaaaatct atgaggaggt attcatcgtg
    10681 aaacgtactt atcaaccaag taaacttcgt cgtgcgcgca aacacggatt ccgtaaccgt
    10741 atgtcaacta aaaatggtcg tcgcgtattg gcagctcgtc gtcgtaaagg acgcaaagtt
    10801 ttggctgtat aatccaaacg aattctatca aaaatcagta ggaactcgag tctactgatt
    10861 tttatttttg taaaaaagtt cagtagatgc aaatggattc ggaagcgatg ttatagtaga
    10921 ttgaaactag aatagtacac ctctgtttct aaaacattgt tagaaatcga tttgactgtc
    10981 ctgatcgatt tgtcctgtta ttattttatt ttactataaa gttgaagtag ctggagatgg
    11041 cacagcaaca atcacattcc cagatggttc agtagtgacg attctaggaa aagatacagt
    11101 tcaacaatct gcgaaaggtg aatctgtaac tcaagaagct acaccagagt ataagccaga
    11161 aactacacca ggtggagata agggaggcaa tactggaaac tcagatgcta atgcgaatgc
    11221 aggcggtggt agccaggcag gtggaccagc tcacacaggt tcacaaaact cagctcaatc
    11281 acaagcttct aagcaattag ctactgaaaa agaatcagct aaaaatgcca ttgaaaaagc
    11341 agccaaggac aagcaggatg aaatcaaagg cgcaccgctt tctgataaag aaaaagcaga
    11401 acttttagca agagtggaag cagaaaaaca agcagctctc aaagagattg aaaatgcgaa
    11461 aactatggaa gatgtgaagg aagcagaaac gattggagtg caagccattg ccatggttac
    11521 agttcctaag agaccagtgg ctcctaagac aacaagtgca ccgcaagcaa ctgcaggaac
    11581 aatgcaagat gttacctacc agtcacctgc tggcaaacaa ttacctaaca caggttcagc
    11641 atcaagtgca gcacttgcta gtcttggtct agtggtggca acaagtggtt ttgctttgct
    11701 aggaagaaag actagacgta gaaaatagaa cagctagaaa attctattct ctacttaaag
    11761 ttagattata agggggattt tgagaagtca tcaatcctag tgatgggtga gaaaagtgag
    11821 aacccaagat aatcacatac tttagctgaa taggaatatt ctatcaatgt agccaatctc
    11881 ttctgtctct aactgtggaa taggagatgg gcaatatcgg atagaaaaga tagcagaata
    11941 gctctctatt gaagagagga ggggaaaccg aaaaattagg tgcccctcct cttttttggt
    12001 ataatagaag gatagaaaac gaggttagaa gagatgattt ttgatacaca tacacacttg
    12061 aatgtagaag aatttgcagg tcgtgaggca gaagaaattg ccttggctgc tgagatgggt
    12121 gtgacacaga tgaatattgt tggttttgat aaaccgacga ttgagcatgc cttggagttg
    12181 gtagatgagt atgagcagct ctatgcgact attggttggc atcctacaga agctggtact
    12241 tatacagagg aagttgaggc ttacttgttg gataagttaa aacattccaa ggttgtggct
    12301 ttaggtgaaa ttggcttaga ctaccattgg atgacagcgc ccaaagaggt gcaggagcag
    12361 gtttttcgcc gtcagattca gctatctaag gacttggatt tgccttttgt tgtccatacc
    12421 cgtgatgcgc tggaagatac ctatgagatt atcaagagtg agggcgttgg tcctcgtggt
    12481 ggtatcatgc attcattttc agggacgctt gagtgggcag agaagtttgt ggatcttggt
    12541 atgaccattt ccttctcagg agtggtgacc ttcaagaagg caactgacct ccaagaagca
    12601 gctaaagagt tacctttgga caagatgttg gtagaaacag atgcgcctta cttagcacct
    12661 gtacccaagc gtggtcgtga aaataaaaca gcctatactc gctatgtggt cgactttatc
    12721 gctgacttgc gtggtatgac gacagaagag ctggcggtag caacgactgc aaatgcagaa
    12781 cgcatttttg gattggacag caagtaatga aagagagaat ttcccaagtt atcgtggttg
    12841 aaggtcgtga tgatacggtc aatctcaagc gttatttcga tgtggagacc tatgagactc
    12901 gaggttctgc catcaatgac caggacatag agcggattca gcgtctacac caacgtcatg
    12961 gagtcattgt ctttacagac ccagatttta atggggagcg gattcggcgc atgattatga
    13021 cggccattcc aacagttcag catgcctttc tcaagcgaga tgaagctgtt cccaagtcca
    13081 agaccaaggg gcgttctctg ggaattgagc atgccagcta tgaagacctg aaaacggctc
    13141 tagctcaggt gacagaacaa tttgaacatg agagtcagtt tgacattagt cgtagcgatt
    13201 tgattcgcct tggttttcta gcaggggcag acagccgtaa gcgtagagaa tatctcggag
    13261 agactctccg aatcggctat tctaacggca agcaactcct caaacgccta gagttgtttg
    13321 gggttacttt ggcagaagtg gaagaagcta tgaaatctta tgagtaggaa agatgtgacc
    13381 gttacaattt tttaagtttc acagtatttt tcgaagcagg tagaagagga ggcgtctgat
    13441 gttaattggt caaaaaatta aagagattcg gatagaaaaa ggaattagtc gtccagattt
    13501 ttgtggagat gagcaaggac tgacagttcg tcaactgtcg cgaattgaaa gtggagcttc
    13561 gcaaccgagt ttgcccaagt tagcctatat tgctcgccgg ctaggagttc cagtttatag
    13621 ccttatgccg gatttttcag ctcttccttc tgcttattta gaattgaaat accagatttt
    13681 acgtgaacca atctatggta aagaagagga gtacgataag aaggaagcgt gtttggaaga
    13741 gatttataaa acatactttg ataatcttcc taaagaagaa caattagcat gtgaagtatt
    13801 gcaggcgtgt ttggatactt ctagaactag aaggcctgaa tatgcagagt taatacttga
    13861 ggaacatatg cctcagatta tagaaaaaga agcttattca ataaatgata tgttgttgat
    13921 tcgtttgttt ttttatcaaa tgctcattag aaaagatctt gccaaattta taaatcaaat
    13981 cgaaaagcta atgctctttc ttttggaaca gaagaaggta actcaaatag agaattactt
    14041 tataattaga gatactctta tttcaggaat gtgttgtctt gaaaaggtag gagtaactga
    14101 ttgttttaat gattatctat cttgtttaca agaaattatg gataaaactc aagattatca
    14161 aaagaaacct cttgtattta tgtttttgtg gaagcaagca ttaagagtag aaagagattt
    14221 tagtttagct gaatcatttt atcagtcttc taaaacattt gcgaagctaa ttggagatgg
    14281 atttctagta aagaaattga cagaggaatg gcaagaggat gtcaaaaaat atttataaac
    14341 atagtgaatc agtgacaaaa atgtccttgt cctcgtatca aaacagttct aaagttcgtc
    14401 tttagaactg tttttttaga tataagctaa aaataatatg aaatagttag attttaagga
    14461 cattgatgtc cttgatagtg tgataaagat ggagtataat gactataaaa gttcatttca
    14521 gggtgctatt ttgacaattt agagaaaaac aaataatttt agaatatcat ttggtaatat
    14581 aatagtatca acgttgataa tggaaagagg agcaagtaac tcaacaatct tgtaatcaaa
    14641 aatagaggag tatcaatatg aaacaaacag ttaaaaaatt agctcttgta gcgagcattg
    14701 cagcaacatt aggtggaagt gtagcagttg cctctgcagc cgttcaatac ccagaaggtg
    14761 gtgtttggac ttatggttca ggtaacggtg gtgcttactc aaactactat cacccttcaa
    14821 aataccatag ttcaacagtt gttagcagaa aaactggttc atctgacaag ggatatgctg
    14881 gtgctggagg gacttctcgt gcatggattc gtacttcttg gggagagaaa gttgcattct
    14941 actataatgt ttagaatggt atagccctat tgtttagctt tacttggtca ccttctcact
    15001 aagtgaccaa gtaacttttt ggaggaaatg atgaaacgtt tatttatttt gatttcaatg
    15061 gtattggtat cgctttatat ggtgataact tccgttgacc atcgagaaga gattttattt
    15121 ggtaactatc cttctgttga tgtgacagga atgatgataa atcaaccagt agctagtcgg
    15181 gaagaggtga cagaggcttt gagtcacttg gcggtagagc acaatagtct cattgctcgt
    15241 cgaatcgttg agccaaatga agctggagaa acacgcttta cctatgccac ttatggtgag
    15301 ggaaagcttc cagaaggtct gaccatttcc tccaaggaga gtgcagaaac gagtgattta
    15361 ttagggtctt acttgattgt gtcaggaagt ttggatggag tgagcttaca gaccaccttg
    15421 aaagagcttg gttatcaagg ctttgtttcg aatggagaag atccattttc gatagtctta
    15481 ctattgacgg ccacccctat ggtgctactg agtttagcta tttttctgct gacctttatg
    15541 agtctgaccc tgatttatcg gatcaaatcc cttcgtcagg cagggattcg cttaatagct
    15601 ggtgagagct tgtttggagt tgctctcaga ccagtgttag aagatgtgag acagcttatc
    15661 tgctcagtgc tggtatccag tcttttggga ttggggattc tctggtatca aggtgccttg
    15721 tttatggcaa cggtgcaact ggtcatcatt gctcttctac tttatggatt gaccttggca
    15781 gggatttcta ccttactaag tgtcgtctat ctacttggtt tacaggaaaa tagtctggtg
    15841 gatctattga aagggaaact ccctctcaaa cgtatgatga cattgatgat ggtggggcaa
    15901 ctcttagctg tattggtggt cggatcgagt gcgacagctc tcctacccca ctaccgtgaa
    15961 atgcaggaaa tggagagagc tagcaataaa tggagccagt cctcagaccg ttaccgtcta
    16021 tcctttggtt ggtctagtgc atttgccgat gaagaaggaa cgcgtaagga taatcgtgag
    16081 tggcagacat ttactgaaga acggttagcc aatacagact ctttttatat tatgagcaat
    16141 gttgacaatt tctcagatgg agcagaagtg gacctagatg gcaatcgtct cagtgactac
    16201 acaccgtcag ggaatgttat ctatgtctca ccacgctatc tgatagaaga aaagattacc
    16261 gtttcttcag agtttatgga caagatgcaa aacttgtctg agggagagtt tgggctgatc
    16321 ttgcctgaga gcttgcgaga gcagtctgtc tactaccaag gattgtttac agattatctg
    16381 caaaactttt catctgaaag tgtagaagtg acgagtcaga aacactacct cccacaggta
    16441 aggctagctt ttacagaaac aggacaggaa cgtttcctct ataatgatgg gtacaagaca
    16501 acacgccagt acctaaaaga tccgattatt gtagttctaa cgccgcaagc gactggagca
    16561 agacctgttg cagggatgtt gtggggaact acggctaata gtgccttgaa actagatcga
    16621 tatggagaca gcatcacagc tctaaaagag caaggtctgt atcacaaggt ttcttacttg
    16681 gtaaaaagcc agctattttt tgccaaggta ctaaatgaca aacgggtgga gttttactct
    16741 ctccttattg ggacgatttt gaccctgtct acggctatct tgttatttga ttccatgaat
    16801 cttctctatt ttgagcagtt cagacgggat cttatgatta aacgtcttgc tggtatgaca
    16861 atctatgagc ttcatggcaa gtatttactg gcgcaaggag gagttctctt gcttggccta
    16921 gtcctatcta gtattttgac aagagatggt ttgattagcg ctctagttgt agctttgttt
    16981 acgcttaacg ccctcttgat tttagtaagg caggacaaaa aagaagaagc tggtagcatg
    17041 gcagtattga aaggaaaata agatgattga tattcaagga ttggaaaaga aatttaatga
    17101 ccgcgcgatt ttctctggtt tgaatctcaa gctggagaag ggcaaggttt atgccttaat
    17161 cggaaagagt ggaagtggaa agacgacgct gctgaatatc ttgggaaagc tagaaaagat
    17221 agatggtgga agggttctct atcaggggaa agatttaaaa accattccca ctcgtgagta
    17281 ttttcgagac cagatgggct atctctttca aaatttcggc ctcttagaaa accaatcaat
    17341 caaagaaaat ttggatttgg gttttgttgg tcagaaaatc tcaaaagtag aacgtttgga
    17401 aaggcaagtg ggggctttag aaaaagttaa tctagggtat ttggatttag aacaaaaaat
    17461 ctatacttta tctgggggag aggcccaacg agttgccctt gctaagacta ttttgaaaaa
    17521 tccacccttg attttggcag atgaaccaac agcagctctt gatcctgaaa attcagagga
    17581 ggttatgaat ctcttggtgg atttgaaaga tgaaaatcga attatcatca ttgcgaccca
    17641 taatccccta gtctggaata aggctgatga aatcattgat atgaggaaac ttgctcatgt
    17701 gtgaaaaaat ccgtattcgc agggtatctg attatcctag tgccagaggt ggtttagaag
    17761 atatcctcat catggaaaat atgaccaatc atctcctttt ggttcaaatc cgagtgcatg
    17821 gctatttgct tgattttgct agtattgaag ggcaaaggca aaagcattat cgtttgaaaa
    17881 atttacctca gacggttgaa ctgacagtgg atgatgtgga ggaggatgtg gatttgaccc
    17941 tacccgaaaa tcgaagttat caagaagctg atttttttga acgcatgttt cgagagaact
    18001 gctaaggcca cttttaaaga tttccaagac tatctttctt catgaggaaa gatagttttt
    18061 tggtatgatt ttcattccca aaatacaagg ggaatgtgtt acaatagtag taacagataa
    18121 tagaaaagag aatagatgag aattgcagat tatagcgtga ccaaggcagt gctggagcgt
    18181 tacggtttta cctttaaaaa gtccttcggg caaaatttcc tgacggatac caatatcctt
    18241 caaaaaattg tggatacggc tgaaattgat gaccaggtca atgtcatcga aatcgggcca
    18301 ggtattggtg ccttgacgga gtttctggct gagtgtgcag cccaagtcat ggcttttgag
    18361 attgaccacc gtttggtgcc aattttggca gatactctgc gtgattttga taatgttact
    18421 gtagtcaacg aggacattct caaggttgac ttggcgcagc atatccagaa ttttaaaaat
    18481 cctgacctgc caatcaaggt agtggctaat ttgccttact acatcacgac gcctattctc
    18541 atgcacttga ttgagagtgg cattcctttt tgtgagtttg tggtcatgat gcagaaagaa
    18601 gtagcggacc gcatttcagc ccagcctaac accaaggctt acggtagctt gtctatcgcc
    18661 gtgcagtatt acatgacagc caaggttgcc tttatcgtgc ctcgtacggt ctttgtgcca
    18721 gcgccaaatg tggattcagc catcttgaaa atggtgcgtc gtccagagcc agccgtagca
    18781 gtagaagatg agaacttttt ctttaaggtt tccaaggcta gttttaccca tcgccgcaag
    18841 accttgtgga ataacttgac aggttacttt ggtaagactg aagaggtcaa ggacaagctg
    18901 accaaggctt tggaccaggc aggcttgtca ccaagtgtgc gtggggaagc tctcagcttg
    18961 gcagaatttg ccggtctagc agacgcactt aaagggcaag gactctaaga tgcagggaca
    19021 aatcattaaa gccttggcag gtttctacta tgtggagagt gatggccagg tttatcaaac
    19081 acgcgcgcgt gggaatttcc gtaaaaaagg ccatacccct tatgttgggg actgggtaga
    19141 tttctctgcc gaggaaaatt cagaaggcta tatcctcaaa attcacgaac ggaaaaacag
    19201 tctggttcgt ccgcctattg tcaatatcga tcaagctgta gtaatcatgt ccgtcaagga
    19261 acctgatttt aacagcaatt tgctggatcg tttcttggtt cttttggagc acaagggcat
    19321 ccatcccatt gtctatattt ccaaaatgga tttgttggaa gataggggag aactggattt
    19381 ttaccagcag acctatggtg acatcggcta tgactttgtg accagtaaag aggaactcct
    19441 gtctttgtta acaggcaagg ttacggtctt tatggggcag acaggtgttg ggaagtcaac
    19501 tcttctcaat aaaatcgcac cagacctcaa tcttgaaacg ggagaaattt cagacagtct
    19561 aggtcgcggt cgccatacca ctcgagctgt tagtttttac aatctcaacg ggggtaaaat
    19621 cgcagataca ccaggatttt catccttgga ctatgaagta tcaagggctg aagacctcaa
    19681 tcaggctttc ccagagattg ctactgttag ccgagattgt aagttccgta cttgtaccca
    19741 tacccatgag ccgtcttgtg ccgtcaaacc agctgttgaa gagggtgtta ttgcaacctt
    19801 ccgttttgac aattacctgc aattccttag tgaaattgaa aatcgtagag aaacctataa
    19861 aaaagtcagc aaaaaaattc caaaataagg agaaacctat gtctcaatac aagattgctc
    19921 cgtcaattct ggcagcagat tatgccaact ttgaacgtga aatcaaacgt ctagaagcaa
    19981 ctggggcaga atatgcccat atcgatatca tggacagtca ttttgtaccg caaatcagtt
    20041 ttggtgcagg tgtggtcgag agccttcgtc ctcatagtaa gatggttttc gattgccact
    20101 tgatggtgtc aaaccctgag catcatctgg aagattttgc gcgtgcaggt gcagacatca
    20161 tcagtatcca tgtagaagca acacctcata ttcatggcgc cctccaaaaa attcgttcac
    20221 tcggagttaa gccttcagtc gttatcaatc ctggcacacc agttgaagcc atcaagcacg
    20281 tccttcatct agttgaccaa gttttagtca tgacggttaa tccaggtttt ggtgggcaag
    20341 cctttctgcc agaaaccatg gataaggttc gtgagttggt tgctcttcgt gaggaaaaag
    20401 gtttgaactt tgaaatcgaa gtggatggtg ggattgatga ccaaactatt gctcaagcca
    20461 aagaagctgg tgcgactgtt tttgtagcag gttcctatgt ctttaaggga gaagtcaatg
    20521 agcgagtaca aactctcaga aaacaactgg actagggttg cagtttttgc aggcggaaac
    20581 cgcggtcatt atcggacaga ttttgatgct tttgttgggg tggatcgagg ctcgctctgg
    20641 gtcttggaag aagacttacc tcttgctcta gcagtcggag attttgattc tgtgacggaa
    20701 gaagagcgac aggtgattca aaaacgtgcc cagtattttg tccaagcacg accagaaaag
    20761 gatgatacag atctggaatt ggctctctta accatctttg aacaaaatcc tcaggctcag
    20821 gtcactattt tcggtgcctt gggtggccgt attgaccata tgttggccaa tgtctttcta
    20881 cctagcaatc ctaagttggc accctatatg cgtcaaatag aaattgagga tgggcaaaat
    20941 ttgattgcct attgttcaga agggaccagt cagcttcaac ctcgttcaga ctacgactat
    21001 ctagccttta tgccagttcg ggatagccag ctgactattc ttggagctaa gtatgagttg
    21061 acagaggaga attttttctt taaaaaagtg tacgcttcta acgaatatat agatagggaa
    21121 gtgtcggtaa cttgcccaga tggttatgtg gtcgtactgc atagcaagga caggaggtag
    21181 gatggaaagt ttacttattc tattattaat tgccaaccta gctggtctct ttctgatttg
    21241 gcaaaggcag gataggcagg agaaacactt aagtaagagc ttggaggatc aggcagatca
    21301 tttgtcagac cagttggatt accgctttga ccaagccaga caagccagcc agttagacca
    21361 aaaagatttg gaagtggctg tcagcgaccg tttgcaagaa gtgcggattg aattgcacca
    21421 aggcctgacc caagtccgtc aagaaatgac agataatctc ctccaaacta gagacaagac
    21481 agaccaacgt ctccaagcct tgcaggaatc aaatgagcaa cgtttggaac aaatgcgcca
    21541 gacggtcgag gaaaaactag aaaagacctt gcagacacgc ttacaggctt cctttgagac
    21601 agtttctaaa caactggagt ctgtcaatcg tggtcttgga gaaatgcaga cagttgcccg
    21661 tgatgtcgga gctcttaaca aggttctctc tggaaccaag acgcgaggga ttctgggaga
    21721 attgcaactg gggcaaatta ttgaagacat catgacacct gcccagtacg aacgagaata
    21781 cgcaacggtt gaaaactcta gtgaacgagt ggagtatgcc atcaagttac ccggacaagg
    21841 cgaccaagaa tacgtctacc taccaattga ctctaagttt ccactggcag attattaccg
    21901 cttagaagaa gcctatgaag taggtgacaa ggatgaaatc gaacgctgtc gtaagtcgct
    21961 cctagcaagt gtcaagcgtt ttgctaggga tattaggaac aagtacatag caccacctcg
    22021 gacaaccaat tttggaatct tgtttgttcc gacagaaggt ctttattcag agattgtgcg
    22081 caatccggtc ttctttgatg atttgagacg ggaagaacag attatcgttg caggaccaag
    22141 taccctatca gcccttctta actccctatc agttggtttc aagaccctta atatccaaaa
    22201 gagtgccgac catatcagca agactcttgc cagtgtcaag accgagtttg gcaagtttgg
    22261 tggtattctg gtcaaggcac aaaaacatct ccaacatgcc tctggcaata ttgatgaatt
    22321 attaaaccgt cgtaccatag ctatcgagcg gacgctccgt cacattgagt tgtcagaagg
    22381 tgagcctgcg cttgatctac tccattttca agaaaatgag gaagaatatg aagattagtc
    22441 acatgaaaaa agatgagcta tttgaaggct tttacctaat caaatcagct gacctgaggc
    22501 aaactcgagc tgggaaaaac tacctagcct ttaccttcca agatgatagt ggcgagattg
    22561 atgggaagct ctgggatgcc caacctcata acattgaggc ctttaccgca ggtaaggttg
    22621 tccacatgaa aggacgccga gaagtttata acaatacccc tcaagtcaat caaattactc
    22681 tccgcctgcc tcaagctggt gaacccaatg acccagccga tttcaaggtc aagtcaccag
    22741 ttgatgtcaa ggaaattcgt gactacatgt cgcaaatgat tttcaaaatt gaaaatcctg
    22801 tctggcaacg gattgtccga aatctctaca ccaagtatga taaggaattc tactcctatc
    22861 cagctgccaa gaccaaccac catgcctttg aaacggggtt ggcttatcat acggcgacca
    22921 tggtacgttt ggcagacgct attagcgaag tttatcctca gctcaataag agtctgctct
    22981 atgcggggat tatgttgcac gacttggcta aggtcatcga gttgacggga ccagaccaga
    23041 ccgagtacac ggtcagaggc aatcttcttg gacatatcgc tctgattgat agcgaaatta
    23101 ccaagacagt tatggaactc ggcatcgatg ataccaagga agaagtcgtt ttgcttcgtc
    23161 atgtcatcct cagtcaccac ggcttgcttg aatatggaag tccagtccgt ccacgtatta
    23221 tggaggcaga gattatccat atgattgaca atctggatgc aagcatgatg atgatgtcaa
    23281 cagctctggc tttggtggat aaaggagaga tgtccaataa aatcttcgct atggataatc
    23341 gttccttcta taaaccagat ttagattaat aatttaagaa aaatgagcat tttttaggat
    23401 aagaatgttc gtttttttat gtgaatatgg tataataagt aaaagacaaa aatgaatact
    23461 cttcgaaaat ctcttcaaac tagggtagta tcgccttgtc gtatgtatat atgcaggtat
    23521 attacagggt ttgtcagttc tattgacaat ctaaaaacag tgttttgaac caccagcgac
    23581 cagctttcta gtttgctttt tgattttttg aataaaaatg gaataggaaa tagaaatgaa
    23641 attaagaaga agtgatcgga tggttgtcat ttccaactat ttgattaata atccttataa
    23701 actaactagt ctcaatactt ttgctgaaaa gtatgagtct gctaaatcat ccatctcaga
    23761 agatatcgtc attatcaaac gcgcctttga ggaaattgaa atcggtcata tccagacagt
    23821 gactggggct ggcggaggtg tcatcttcac accgtctatt tcgagtcagg atgctaagga
    23881 aatggttgaa gacttgcgta ccaagttgtc agaaagtgac cgtatcttgc caggtggtta
    23941 tatctatctg tctgatttgc ttagcacacc agccatcttg aaaaatattg gtcgtattat
    24001 tgccaaaagc tttatggacc aaaaaattga cgcggttatg accgtagcaa ctaagggtgt
    24061 gccacttgca aatgcagttg ccaatgtcct caatgtctct tttgtcattg tgcgccgtga
    24121 cctgaaaatt accgaaggtt caactgttag cgtcaactat gtttcaggtt caagtggtga
    24181 ccgtatcgag aaaatgttcc tttcaaaacg tagtcttaag gcaggcagcc gtgtcttgat
    24241 tgtggatgac ttcttgaaag gtggcggaac ggtcaatggt atgattagtc tcttgcgcga
    24301 gttcgactca gaactggcag gtgtagcggt ctttgcggac aatgcccaag aagaacgtga
    24361 aaagcagttt gactacaagt cactcttgaa ggtaaccaat attgatgtca agaaccaagc
    24421 catcgatgtt gaggttggca atatctttga cgaagataaa taagagatag aactaaaggt
    24481 tggaacgatt gtcccagcct ttctttgcaa acagaataga aggaagctta tgaaaacacc
    24541 atttatcaat agagaagagt tagaagcgat tgttgccgag ttcccgactc cctttcactt
    24601 gtatgatgag aaggggattc gtgagaaggc aagagccgtc aaccaagctt tttcgtggaa
    24661 caagggcttt aaggaatatt ttgcagttaa ggctactcca actccagcta ttttgaaaat
    24721 tctccaagaa gaaggttgtg gtgtggactg ctctagttat gtagagcttt tgatgagcca
    24781 taaactggac tttctgggtt ctgagattat gttctcttcc aacaacacgc cagacaagga
    24841 atacgcctat gcacgtgaat tgggtgcgac cattaacttg gatgcctttg aagatattga
    24901 acatctggag agagtagcag gcattccaga aatcatctct tgtcgttata atcctggagg
    24961 cgtttttgaa ctggggacag acattatgga caatcctggg gaggctaagt ttggcatgac
    25021 caaggaccag ctctttgaag cctttgctat cttgaaggaa aaaggagcca agacttttgg
    25081 gattcactcc ttcctagcgt ccaataccgt gacccatctc tattatccag agttggctcg
    25141 tcagctcttt gaactggctg ttgaaatcaa ggaaaagttg ggcatttcgc tagactttat
    25201 caatctttct ggcggtattg gtgttaatta tcgtccagac caggagtcga acgatatcgc
    25261 cttgattggt gagggagttc gtaaggtgta tgaagaagtt cttacgtcag caggtcttgg
    25321 tcaggtcaag attttcaccg aattgggtcg ttttatgctg gcacctcacg gtgctctagt
    25381 cacaagagtg actcataaga aggaaaccta ccgtacctat ctaggtgtgg atgcctcagc
    25441 agtcaacctc atgcgtccag ctatgtacgg agcttaccat catattagca acgtgaccca
    25501 tccagatgga ccagctgaag cggtagatgt ggttggttca ctctgtgaaa acaatgataa
    25561 atttgcagtc aatcgcgaac tgcctcatac agaaatcggt gatttgctgg tcattcatga
    25621 tacaggtgct cacggttttt caatgggtta ccagtacaac gccaaacttc gttctgcgga
    25681 aatcctctat accgaagaag gtgaagctcg ccaaatccgc cgtgcagagc gccctgagga
    25741 ctattttgca accttatatg gattcgattt tgaagaataa tctgataata gcttgaaaat
    25801 gaaattgaaa aacagattgc tttctaaaaa ataggcaaaa atcttgtttt tccttcaagt
    25861 cgtgatataa taaaactata aaacgttttc aaggaaggta acgatatgtc tgaagaaaca
    25921 attgattatg gacaagtgac aggaatggtg cattcgacag aaagctttgg gtcagtagat
    25981 gggcctggta ttcgctttat tgtctttttg cagggctgtc acatgcgttg ccagtattgc
    26041 cacaacccag acacttgggc tatggagtcc aataagtcac gtgaacggac ggtagatgat
    26101 gtcttgacag aggccttgcg ctaccgtggt ttctggggaa ataagggtgg gattacagtc
    26161 agtggaggag aagctctctt gcagattgat ttcctgattg ccctcttcac caaggctaag
    26221 gaacaaggaa tccactgtac cttggatacc tgtgctcttc ctttccgtaa taaaccacgt
    26281 taccttgaga agtttgacaa actcatggct gtcactgact tggttctttt ggatatcaag
    26341 gaaatcaacg aagaacagca caagattgtc actagccaaa ccaataaaaa tatcttggct
    26401 tgtgcccagt atctatcaga tattggaaaa cctgtctgga ttcgccacgt gctagttcca
    26461 ggattgacag acagagatga tgacttgatt gaacttggta agttcgtcaa gaccctcaaa
    26521 aatgttgata agtttgaaat tctaccttat cacaccatgg gtgagttcaa gtggcgtgaa
    26581 cttggaattc catattccct cgaaggagtc aaaccaccaa cagcagatcg cgtcaagaac
    26641 gctaaaaaac tcatggatac cgaaagttat caagattata tgaaacgtgt acatggatag
    26701 aaaagaagcc tgatggaaac atcgggcttt tgacttgcaa aaagacttag caaatcagct
    26761 aagccttttt cttcttatct cgaacgttgt tttccagcgt tgcgattttt gtgttttttc
    26821 ttgcttgtaa tagcagttgg ttgttcaggg gtaacgtctt ttcgtccact tggtgttgag
    26881 aaagcacttg cttttggtgg gttctttgcc agttcttcac ggactttttt gcgaagtttt
    26941 ggacgaacga tatagttgac gataaactgt tggagaatca tcatgaaacc accgacaacc
    27001 cagtaaagtg tgacactagc tggtgagaag agggagaaga cgacgatcat gagtgggctc
    27061 atgtaaatca ttttcttgat ttgttctctt tgcatttcat cttctacccc gtgaagtgaa
    27121 aggagcgatt gaagatagta aaggacacca gcacaggcaa ccaaaatcat acttggagaa
    27181 cctagaggaa tgcctaggta gcttgcttga gcaacccctt cagtatgttg ggcagcaaag
    27241 tagatagcag agaagaaagg catttgaagg aggataggga aacatcctac accgccaaac
    27301 atgctgatac cgtgctcttt ttgagcagca aagagagctt gttgggcttc gagtttttct
    27361 tcttgagtag tcgcttcttt gagacgcgtt tggtgtggct caaggacgtg cttgagggcg
    27421 ttcatctttt cagagtgaag cgttgccttc catgattggt agataccaag tggcaagata
    27481 atcaagcgca cgataatggt tacgatgatg atagcgacac caaagcctag acctttatca
    27541 gtagcgaagt acttgatagc ttcagccata ggggctccga tggtattcca aataaatcct
    27601 gttggctgac ctgtggtttt atcgacattg acacaaccag tcaagacaag caacatagcc
    27661 gctcccatag ctgagagtgc aaaacgttta atagatttca ctgtttttat tccttcttta
    27721 aaaattatac ctttctattc tactgttttt ttacaaaata tacaatagtt ctggagacct
    27781 aatttgcgat tttaaagtca gagtagggag gaaagttgct tagtttgaca tctaagtagc
    27841 ttacttttga aaaaggtgtc ggtccttttc ggatttcttg gataaatttt gccatgatag
    27901 cagatgagtc tgcttgggct aagatttcca ctgtgccatc gtcgttattc catacccgac
    27961 ctgtgatgcc accgatttca agggccaggc tataaacacc ccaacgaaag ccgactccct
    28021 gcaccctacc ttgggcaatc attctaacct tttgcatacc aaaccccttt gattgtgtta
    28081 taatacttct atgactatta taacctcaaa agccaattct gtggtaaaaa atgccaagaa
    28141 attacaccaa aaaaaatacc gcaagtctgc ctatttgatt gaaggctggc acttgtttga
    28201 agaagctgtc caagctggag tgacgattga gaaaatcttt gccctagaaa attatcgaaa
    28261 tcagttagct gcttttccgc aaactgtctg gatttcagag gatattttgc tagatttggc
    28321 ggattctcag actccacagg gaattgttgc cgtggttcaa aaagaagaag taggacaagc
    28381 tgatttgagt cagggcaagt tcttgttttt ggaagatgtg caagatcctg gtaatgtagg
    28441 aactatcatt cgaactgcgg atgcagcagg ttttacagga gtgattgttt cagataagtc
    28501 ggcagacatc tacagtctca aaaccctacg ttccatgcaa ggtagtcatt tccatctgcc
    28561 catttaccgg atgactagtc aagcgcttct tgacgaaact aaaaaggtag ctatcccagt
    28621 gctagcaaca accctatcta aagattctgt tgattacaga gaactgcctt ctatagaaaa
    28681 ttttgtacta gttatgggaa atgagggtca aggaattagt ccccttatgg ctgaaagtgc
    28741 agaccagttg gtccatatta gcatgaaggg gcaggccgag agtttgaatg ttgcagttgc
    28801 aaccggtatt ttaatcttcc atttaagcta attttaactt tttttgttat aatcaaggaa
    28861 agatgttcac agaaaaggag aaaaggatga atcacactat tatacatgac cgtgcaggtc
    28921 tcaatcaatt ttacgctaag gtttatgcct ttgttggtct gggaatcgga ctatctgctt
    28981 tggtatcagg ccttatgttg acggtctttc agtctcagtt ggtttacttg ttgatgcagg
    29041 ggcgtctctg gttgaccatt gctacttttg cggagttggc tttggttttc gttgccagta
    29101 gcatggcttc aagaaatagt ccagctgctc ttccagtatt tttactttac tccgttttaa
    29161 acggctttac ccttagtttt gtggtggctt tctatactcc gggtacagtt ttatctgcct
    29221 ttgtatcgag cgcccttctc ttctttgtca tggcggcagt tggtattttt accaagaaag
    29281 acttgagtgg gattggtcga gcaatgatgg cagctctcat tggtttactg attgctatgg
    29341 tagtcaatat tttcttggct agtggcttct ttgattatat gattagcgta gccatggttt
    29401 tggttttttc agggttgatt gcttgggata accaaaagat tcgcctcgcc tatgaacaat
    29461 cacaaggtcg tgtagcgaca ggctgggtgg tgtcaatggc tctcagcatc tatcttgatt
    29521 ttatcaatct tttcctaagt attctacgta tctttggtcg caatgactaa aaggtctatg
    29581 aaatcagtgt tttgagtgac actgattttt tttaaatttt ctcttttctt ttcttggaaa
    29641 taggtgtata atacgtttat taaatttttg aggagttgtc tatgaagaaa agttttatcc
    29701 atcaacaaga agaaatttcc tttgtcaaaa acacttttac ccagtatttg aaagataagc
    29761 tagaagttgt cgaagttcaa ggtcctatct tgagtaaggt cggtgacgga atgcaggaca
    29821 acctgtctgg tgtggaaaat ccagtatcgg tcaaggttct ccaaatccct gatgctactt
    29881 atgaagtggt gcactcactt gctaaatgga aacgccacac cttggctcgt tttggctttg
    29941 gtgaaggaga gggtctcttt gtccacatga aagcccttcg tccagatgag gattccttgg
    30001 atgcaaccca ctctgtttat gttgaccagt gggactggga gaaggttatt ccaaatggta
    30061 agcgtaacat cgtttatcta aaagaaacag ttgagaagat ttataaggct attcgcctga
    30121 ctgagctagc tgttgaagcc cgctatgaca tcgagtctat cttgccaaaa caaatcacct
    30181 ttatccatac agaagaattg gtagaacgct acccagactt gacacctaaa gaacgtgaaa
    30241 atgcgatttg taaagaattt ggagccgtct ttttgattgg tatcggtggc gagttgccag
    30301 atggtaaacc gcacgatgga cgtgcaccag actatgatga ctggacaagc gagtctgaga
    30361 atggctacaa gggtctaaat ggtgatattc ttgtctggaa tgagtcttta ggtggagcct
    30421 ttgagttgtc ttctatggga attcgtgtag atgaagaaac tcttagacgt caggtggaaa
    30481 ttacaggtga tgaagaccgc ttagaattgg aatggcacaa gtctttgttg aatggcctat
    30541 tcccattgac aatcggtgga ggaattggac aatctcgtat ggccatgttc ctacttcgca
    30601 agagacacat cggagaagtg caaacaagtg tttggcctca agaagtccgc gatacttacg
    30661 aaaatatttt gtagagaatc gaaccgcaag gttcggtttt ctttctcttt ttgtctataa
    30721 tttggtataa taaacagtat gaaaatcgta tcaggaatct atgggggacg tcccctcaag
    30781 acactagaag gcaagacaac aagacctact tcggataagg ttaggggagc catttttaac
    30841 atgattggtc cctactttga agggggacga gtcttggacc tttatgcagg tagtggtggt
    30901 ttatctatcg aagcagtatc gcgtggcatg tccaatgctg ttttggtgga gcgaggccgt
    30961 aaggctcaga ccatcgtggc tgaaaatatc cagatgacca aggaaattgg aaaatttcaa
    31021 ctcctcaaga tggatgcaga aagggcattg gaacgggtat ctggagaatt tgacctcgtt
    31081 ttcttagacc ctccctatac caaggaacaa atcgtagcag atattgaaaa aatggctgag
    31141 agagagcttt tttctgaaga tgttatggtt gtgtgcgaga cggataaggc cgttgaactt
    31201 ccagaagaaa ttgcctgtct gggtatctgg aaggaaaaga tttatggaat tagtaaggtg
    31261 acagtctatg tcagataaga ttggcttatt cacaggctca tttgatccga tgacaaatgg
    31321 gcatctggat atcattgaac gggcgagcag actctttgat aagctctatg tcggtatttt
    31381 ttttaatccc cacaaacaag gatttcttcc tatcgaaaat cgtaaacggg ggttagaaaa
    31441 ggctgtgaaa catttgggaa atgttaaagt cgtgtcttct catgatgaat tggtggtcga
    31501 tgtcgcaaaa agactggggg ctacttgcct agtgcgaggc ttgagaaatg cgtcggattt
    31561 gcaatacgaa gctagttttg attactacaa ccatcagctg tctcctgata tagagactat
    31621 ttatttacat agtcgacctg aacatctcta tatcagttca tcaggcgtta gagagctttt
    31681 gaagtttggt caggatattg cctgctatgt tcccgagagt attttggagg aaataagaaa
    31741 tgaaaaaaaa gattagatgg cccttatacg tcattgcggc cttgattgtg actttcttgg
    31801 catttgtagt gcccttgcct tattatatag aggttccagg tggttcggaa gatattcgcc
    31861 aagtccttaa agtaaatgac acagaagata aggaagctgg tgcctatcaa ttcgttacgg
    31921 ttggtgttca acatgccact ttagctcata tgatttatgc ttggttgaca ccttttacag
    31981 atattcgtag tgctcaggag actacaggtg gttcttccga tgttgaattt atgcgaatca
    32041 atcaattcta catgcaaaca tcacaaaata tggccaagta tcaaggacta aaaacagctg
    32101 gtaaggatat cgaactcaag tactttggag tttatgtttt gaatgtgacg gataattcaa
    32161 cctttaaagg gattctcaat atctctgata cggtcacagc agtcaatgat cagacctttg
    32221 atagttccaa agacttgatt gattacgtca gttctcaaaa attaggggac tccgtcaagg
    32281 tcacctatga agaggatggg caaaccaagt ctgcagaagg aaagattatc accttggaaa
    32341 atggaaaaaa tgggattgga atcggcttga ttgatcgtac agaggtaatc agcaatgtcc
    32401 caattagctt ttcaacagct ggtattggcg gcccaagtgc tggtctcatg tttagtctag
    32461 ctatctatac tcaaatagct cacccagatc ttcgtaatgg tcgtattgtt gccggtacag
    32521 gtaccattga ccgcgatggt aatgtgggag acattggagg tattgataag aaggttgtag
    32581 cttcggctag ggcaggtgct gctattttct ttgctcctga taaccctgtt agcgaagaag
    32641 aacaaaaggc gcatccggac gtgaaaaaca actaccaaac agccctagaa gcggctaaaa
    32701 caatcaagac ggatatgaaa atcgtgcccg ttaaaaccct acaagatgcg attgattact
    32761 tgaaaaacaa tccctaattt ttgagtgaag tttccaaact agcgcacaaa cagagaagat
    32821 ggtataatag tcaaatggtt caattattat tcactctaag tagtcatatg ctctttattt
    32881 atgtgagttt ttacctttta aaggatcttg ttagatggga gaaggtttta aaagtgacag
    32941 atgagaatac aagaaaagtt cgttcattag tagccttttt tagcattgtc ataggctaca
    33001 tcctgagttc tttctttatt agcctgtatc atttgtggca agaagcgctt agaggattat
    33061 tataaaatca agagtaaagg aaacgagtat ggataaaatt gtggttcaag gtggcgataa
    33121 tcgtctggta ggaagcgtga cgatcgaggg agcaaaaaat gcagtcttac ccttgttggc
    33181 agcgactatt ctagcaagtg aaggaaagac cgtcttgcag aatgttccga ttttgtcgga
    33241 tgtctttatt atgaatcagg tagttggtgg tttgaatgcc aaggttgact ttgatgagga
    33301 aactcatctt gtcaaggtgg atgctactgg cgacatcact gaggaagccc cttacaagta
    33361 tgtcagtaag atgcgcgcct ccatcgttgt attagggcca atccttgccc gtgtgggtca
    33421 tgccaaggta tccatgccag gtggttgtac gattggtagc cgtcctattg atcttcattt
    33481 gaaaggtctg gaagctatgg gggttaagat tagtcagaca gctggttaca tcgaagccaa
    33541 ggcagaacgc ttgcatggcg ctcatatcta tatggacttt ccaagtgttg gtgcaacgca
    33601 gaacttgatg atggcagcga ctctggctga tggggtgaca gtgattgaga atgctgcgcg
    33661 tgaacctgag attgttgact tagccattct ccttaatgaa atgggagcca aggtcaaagg
    33721 tgctggtaca gagactataa ccattactgg tgttgagaaa cttcatggta cgactcacaa
    33781 tgtagtccaa gaccgtatcg aagcaggaac ctttatggta gctgctgcca tgactggtgg
    33841 tgatgtcttg attcgagacg ctgtctggga gcacaaccgt cccttgattg ccaagttact
    33901 tgaaatgggt gttgaagtaa ttgaagaaga cgaaggaatt cgtgttcgtt ctcaactaga
    33961 aaatctaaaa gctgttcatg tgaaaacctt gccccaccca ggatttccaa cagatatgca
    34021 ggctcaattt acagccttga tgacagttgc aaaaggcgaa tcaaccatgg tggagacagt
    34081 ttttgaaaat cgtttccaac acctagaaga gatgcgccgc atgggcttgc attctgagat
    34141 tatccgtgat acagctcgta ttgttggtgg acagcctttg cagggagcag aagttctttc
    34201 aactgacctt cgtgccagtg cggccttgat tttgacaggt ttggtagcac agggagaaac
    34261 tgtggtcggt aaattggttc acttggatag aggttactac ggtttccatg agaagttggc
    34321 gcagctaggt gctaagattc agcggattga ggcaagtgat gaagatgaat aagaaatcaa
    34381 gctacgtagt caagcgttta cttttagtca tcatagtact gattttaggt actctggctc
    34441 taggaatcgg tttaatggta ggttatggaa tcttgggcaa gggtcaagat ccatgggcta
    34501 tcctgtctcc agcaaaatgg caggaattga ttcataaatt tacaggaaat taggctggag
    34561 aaccagcctt tttctaaaga taaggagaaa tatgaacaaa aaaacaagac agacactaat
    34621 cggactgcta gtgttattgc ttttgtctac agggagctat tatatcaagc agatgcagtc
    34681 ggcacctaat agtcccaaaa ccaatcttag tcagaaaaaa caagcgtctg aagctcctag
    34741 tcaagcattg gcagagagtg tcttaacaga cgcagtcaag agtcaaataa aggggagtct
    34801 ggagtggaat ggctcaggtg cttttatcgt caatggtaat aaaacaaatc tagatgccaa
    34861 ggtttcaagt aagccctacg ctgacaataa aacaaagaca gtgggcaagg aaactgttcc
    34921 aaccgtagct aatgccctct tgtctaaggc cactcgtcag tacaagaatc gtaaagaaac
    34981 tgggaatggt tcaacttctt ggactcctcc aggttggcat caggtcaaga atctaaaggg
    35041 ctcttatacc catgcagtcg atagaggtca tttgttaggc tatgccttaa tcggtggttt
    35101 ggatggtttt gatgcctcaa caagcaatcc taaaaacatt gctgttcaga cagcctgggc
    35161 aaatcaggca caagccgagt attcgactgg tcaaaactac tatgaaagca aggtgcgtaa
    35221 agccttggac caaaacaagc gtgtccgtta ccgtgtaacc ctttactacg cttcaaacga
    35281 ggatttagtt ccctcagctt cacagattga agccaagtct tcggatggag aattggaatt
    35341 caatgttcta gttcccaatg ttcaaaaggg acttcaactg gattaccgaa ctggagaagt
    35401 aactgtaact cagtaaaaga taagcctaaa ctcctatgtc acttatggat gtaggagttc
    35461 tttttactag tttaagcagg gctagaacag gtactaagaa aaaatagtaa cttctaaaac
    35521 taacttccag tttcccacaa cctagctttt agtatgagaa aaacgcgtga aatcagtgga
    35581 aatccgtctt agactaactt ccagttttgg gagagagatg gaagttacgc taagaaaaaa
    35641 ataatatgta ttggattaaa ttttgtgata taataaagga taagatacta ttttaggaga
    35701 agaactatgg aagacccaag tagtcagaat ttgttgctac aatttgtttt attgtttatc
    35761 ttgacggtat taaatgcctt tttctcagcc acagaaatgg ccatggtttc tcttaatcgt
    35821 gcccgagttg aacaaaaggc ggaagaagga gacagacgct acatccgcct gctgaaggta
    35881 ctagaaaatc ctaaccactt tttatcaacc attcaggtag gaatcaccct gattacaatc
    35941 ttatcagggg cgagtttaac tgatactcta ggacgtgtga ttgcctcttg gcttgggaat
    36001 ggcgaaacag cacaagccgt ggcaactttt ctatccttgg catttttgac ctatatttcc
    36061 atcgtttttg gggaattata tcctaagaga atcgctctta atctaaagga tgccttggca
    36121 attcgtacag cgccgattat cattgggatc ggaaaactag tcagtccttt tgtttggctt
    36181 ttagctgctt cgaccaattt cttgagccat ttgactccta tgtcattcga tgatgcggat
    36241 gaaaagatga cccgtgatga aattgcctat atgctgacaa atagtgaaga aacattggat
    36301 gctgatgaga ttgagatgct acaaggtgtc ttttcgctcg atgaactgat ggcacgagag
    36361 gttatggttc ctcgaacgga tgcctttatg gtggatattc aggatgatag tcaagccatt
    36421 atccaaagta ttttaaaaca aaattattct cgtatcccag tttatgatgg ggataaggac
    36481 aatgtaattg gaatcattca caccaagagt ctccttaagg caggctttgt ggacggtttt
    36541 gacaatattg tttggaagag aattttacaa gatccacttt ttgtacctga aactattttt
    36601 gtggatgact tgctaaaaga actgcgaaat acccaaagac aaatggcaat cttgctggat
    36661 gaatacggtg gtatggctgg tttggtgact ttggaagacc tcttagagga aattgttggt
    36721 gaaattgatg atgaaacgga caaagcagca atagatgttc atcagattgg cgaggatacc
    36781 tacattgttc aaggaactat gaccctcaat gatttcaata attactttga tgttgaactg
    36841 gaaagtgatg acgttgatac catcgctggt tattatttga caggagtggg aaccattcca
    36901 acgactgaga aactcagcta tgaattggtc agccaaaaca aacagtttat cttaaccaat
    36961 gataaagtga agaatggacg tgttaccaag gtaaaagtcc aaatcacaga agtcgaaata
    37021 gaagaagaaa cagaataaac tagaggcttt tgtcaccagg cgtgacgaag cttttttcaa
    37081 ttgatttcaa aagggaatta tgccatggaa agaactctgc cacaaactcg ctccaaaagt
    37141 ttttaaagtt atacgtattt attcgagaga aaataaaaaa agcccttcaa attgggcttt
    37201 ctgttcattt gaaacctgat aaatcaagtt atagaaggcg gtagacggat ttgaaccgac
    37261 gatcaagctt ttgcagagcc gtgccttacc acttggctat accgccttaa cgtttattat
    37321 tataccttga aaatgatttt cagtcaatag ttttttcagt tttttgtacc atagcgtgat
    37381 tcgaatgtga tgccaagttt tctatgggaa aaaaattacc aaaaacctac tgaatcccct
    37441 tgaaaaatct agctaaatag ggtataatat gggtaatcat ttgtcgtagg ttttgtctga
    37501 aatattgtcc agacaaggct cacagcagtt aaatcttctg aaaaagtcag atttagctgc
    37561 tctttttgtg ctttttttca agattttgag catttgtaac agaggcttaa agattctgaa
    37621 aattcgtcaa gaggacacgg tgataagggg tttacaacca tatggcgatt agaaaagcct
    37681 gattgacaag gcttggaact tatttacaaa ggagaatcat cttggcagga catgacgttc
    37741 aatacgggaa acatcgtacc cgtcgtagtt tttcaagaat caaagaagtt cttgacttac
    37801 caaatttgat tgaaattcaa actgactcat tcaaagcttt cctagaccac ggtcttaagg
    37861 aagtgtttga agatgtattg ccaatttcaa acttcacaga cacaatggag ttggaatttg
    37921 ttggatatga aatcaaggaa ccaaaataca cgctagaaga agctcgtatc cacgatgcta
    37981 gctactcagc accaattttt gtaaccttcc gcttgatcaa taaagaaaca ggcgaaatca
    38041 agacccaaga agttttcttt ggtgatttcc caatcatgac agaaatgggt actttcatca
    38101 tcaatggtgg tgaacgtatt atcgtttctc agttggtccg ctcaccaggt gtttacttta
    38161 acgacaaagt agacaaaaat ggtaaggtgg gctatggttc aactgttatc cctaaccgtg
    38221 gagcttggtt ggaacttgaa agcgactcaa aagatatcac ctacactcgt atcgaccgta
    38281 ctcgtaagat tccatttaca accttggttc gtgctcttgg tttctcaggt gatgatgaaa
    38341 tctttgatat ctttggtgac agcgaattgg ttcgcaacac tgttgaaaaa gatatccaca
    38401 agaatccaat ggactctcgt acagacgaag ccttgaaaga aatttacgaa cgccttcgtc
    38461 caggtgagcc taagacagct gaaagctcac gtagcttgct tgtggctcgc ttctttgacc
    38521 cacgtcgcta tgacttggca gcagttggtc gttacaaaat caataaaaaa ctcaatgtta
    38581 aaacacgttt gctcaaccaa accattgcag agccattggt agaccctgaa actggagaaa
    38641 tcttggtaga agctggtacg attatgactc gtagcgtgat tgaaagcatt gaaagccatt
    38701 tggatggcga cttgaacaag attgtctaca tcccaaacga tgcagccgtt gtgactgagc
    38761 ctgttgttct tcaaaaattc aaggttattg ctccaactga tccagatcgc gtcgtaacga
    38821 tcattggtaa tgctaaccca gatgacaagg ttcgtacggt gactcctgca gatatccttg
    38881 ctgagatgag ctacttcctc aacttggctg aaggacttgg ccgtgtagat gatatcgacc
    38941 accttggaaa tcgtcgtatc cgtgcggttg gtgaattgct tgccaaccaa gtacgtttgg
    39001 gactttctcg tatggaacgt aatgtccgtg aacgtatgtc tgttcaggac aatgaagtct
    39061 tgacaccaca acaaattatc aatatccgtc ctgtaacagc tgcagttaaa gaattctttg
    39121 gttcatcaca gttgtcacag ttcatggacc aacacaaccc gctttctgag ttgtctcaca
    39181 aacgccgttt gtcagcctta ggacctggtg gtttgactcg tgaccgtgct ggatatgaag
    39241 tacgtgacgt gcactacact cactatggtc gtatgtgtcc aatcgagaca cctgaaggac
    39301 ctaacatcgg tttgatcaat aacttgtcat cttacggaca cttgaacaaa tatggttttg
    39361 ttcaaacacc ataccgtaag gttgaccgtg aaacaggtgt tgtcacgaac gaaattgttt
    39421 ggttgacagc tgatgaagaa gatgaatata ctgtagctca ggctaactct cgtctgaatg
    39481 aagatggaac ctttgctgag aagattgtca tgggacgtca ccaaggggtc aaccaagagt
    39541 atccagctaa tattgttgac tacatggacg tttcaccaaa acaggtagtt gccgttgcga
    39601 cagcatgtat tcctttcttg gaaaacgatg actccaaccg tgccctcatg ggagccaata
    39661 tgcaacgtca ggctgtgcca ttgattaatc ctcaggcacc ttacgttggt actggtatgg
    39721 aataccaagc agcccacgat tctggtgcgg ctgtgattgc tcagtatgat ggtaaagtta
    39781 cttacgcaga tgctgacaag gtagaagttc gtcgtgaaga tggttcattg gatgtttacc
    39841 acatccaaaa attccgtcgt tcaaactcag gtactgctta caaccaacgc actctcgtaa
    39901 aagttggtga tgtcgttgaa aaaggcgatt tcatcgctga cggaccttct atggaaaatg
    39961 gagaaatggc gcttggacaa aacccaatcg ttgcctacat gacttgggaa ggttacaact
    40021 tcgaggatgc cgttatcatg agcgaacgct tggtgaagga cgatgtctac acatctgttc
    40081 accttgaaga atacgaatca gaaacgcgcg atacaaagct tgggcctgaa gaaatcactc
    40141 gcgaaattcc aaacgttggt gaagatgccc tcaaagacct tgacgaaatg gggattatcc
    40201 gtattggtgc tgaggttaaa gaaggtgata ttcttgtagg taaagtaaca cctaagggtg
    40261 agaaagatct ttcagctgaa gaacgtctct tgcacgctat ctttggagac aagtctcgtg
    40321 aagtgcgtga tacttctctt cgtgtaccac acggtgccga tggtgtcgtt cgtgatgtta
    40381 agatctttac acgtgtaaat ggagatgagt tgcaatcagg tgttaacatg ttggttcgtg
    40441 tttacatcgc tcaaaaacgt aagattaagg tcggagataa aatggccgga cgtcacggaa
    40501 acaaaggggt tgtctctcgt atcgttcctg tagaagacat gccttacctt ccagacggaa
    40561 ctccagtcga catcatgttg aacccacttg gggtgccatc acgtatgaat atcggtcagg
    40621 ttatggagct tcaccttggt atggcagctc gtactcttgg tattcacatt gcgacaccag
    40681 tctttgatgg agcaagttct gaagatcttt ggtcaactgt taaagaagca ggtatggata
    40741 gcgatgccaa gacaatcctt tacgatggac gtacaggtga accatttgat aaccgtgttt
    40801 ctgttggagt catgtacatg atcaaactcc accacatggt tgacgataaa ttgcatgcgc
    40861 gttcagtcgg accttactca actgttaccc aacaaccact cggaggtaaa gctcagtttg
    40921 gtggacaacg tttcggtgag atggaggttt gggctcttga agcctacggt gcgtcaaatg
    40981 tccttcaaga aatcttgact tacaagtcgg acgatatcaa cggacgtttg aaagcctatg
    41041 aagctattac aaaaggcaaa ccaattccaa aaccaggtgt tccagaatcc ttccgagttc
    41101 ttgtcaaaga attgcaatct cttggtcttg acatgcgtgt cctagacgaa gatgaccaag
    41161 aagtggaact tcgcgacttg gatgaaggaa tggacgaaga tgtcatccac gtagatgacc
    41221 ttgaaaaagc ccgcgaaaaa gcagcccaag aggctaaagc agcctttgaa gctgaagaag
    41281 ctgagaaagc aacaaaagcg gaagcaacag aagaagctgc tgaacaagaa taagcagttc
    41341 acttagaata gaaagggaag aaatagtggt tgatgtaaat cgttttaaaa gtatgcaaat
    41401 caccctagct tctccaagta aagtccgttc atggtcttat ggagaagtca aaaaacctga
    41461 aacaatcaat tatcgtacct tgaaaccaga acgtgaagga ctctttgatg aagtgatctt
    41521 tggtcctaca aaagactggg aatgtgcttg tggtaagtac aaacgtattc gttacagagg
    41581 aattgtttgt gaccgctgtg gggttgaagt aacgcgtacg aaagttcgtc gtgagcgtat
    41641 gggacatatc gaattgaaag ctcctgtatc tcacatctgg tacttcaagg ggattccaag
    41701 ccgtatgggc ttgacccttg atatgagccc tcgtgccctc gaggaagtta tctactttgc
    41761 ggcttatgtg gtgattgatc ctaaggatac accacttgag cacaagtcta tcatgacaga
    41821 gcgcgaatac cgagagcgct tgcgtgaata tggttatggt tcatttgttg ccaagatggg
    41881 tgcggaagcc atccaagacc ttttgaagca agtagatctt gaaaaagaaa ttgctgaact
    41941 caaagaagaa ttgaaaacag ctactggaca aaaacgtgtc aaagccatcc gtcgtttgga
    42001 tgttttggat gccttttaca agtctggaaa caaacctgaa tggatgattc ttaacatcct
    42061 tccggttatc ccaccagatc ttcgtccaat gttgcagttg gatggtggcc gttttgcctc
    42121 atctgacttg aatgaccttt accgccgtgt tatcaaccgt aacaaccgtt tggctcgttt
    42181 gcttgagtta aatgcaccag gtatcatcgt tcaaaatgag aagcgtatgc ttcaagaagc
    42241 agttgacgct ttgattgaca atggtcgtcg tggtcgtcca atcacaggac caggtagccg
    42301 tccattgaaa tcattgagcc acatgcttaa aggtaaacaa ggacgcttcc gtcaaaactt
    42361 gctcggtaaa cgtgttgact tctcaggacg ttccgttatc gccgttggtc caactcttaa
    42421 gatgtaccaa tgtggtgtgc cgcgtgaaat ggcgattgaa ctctttaaac catttgtcat
    42481 gcgtgaaatc gttgcccgtg atatcgtgca aaacgtcaaa gcagctaaac gcttggtgga
    42541 acgcggagat gagcgtatct gggatatcct tgaagaagtg attaaagaac acccagtgct
    42601 tttgaaccgc gcaccgaccc ttcaccgttt gggtatccaa gccttcgagc cagtcttgat
    42661 tgatggtaag gctcttcgct tgcacccact tgtctgtgaa gcctacaatg ctgactttga
    42721 cggggaccaa atggccatcc acgtaccact ttcagaagaa gcacaagcag aagctcgtat
    42781 cctcatgcta gctgctgagc acatcttgaa cccgaaagat gggaaaccgg tagttactcc
    42841 atctcaggac atggttttgg gtaactacta cttgaccatg gaagaagctg gtcgcgaagg
    42901 tgaaggaatg gtcttcaaag accgtgacga agcggttatg gcttaccgca atggttatgt
    42961 tcacctccac tcacgtgttg gtatcgcaac agacagcctc aacaagcctt ggacagaaga
    43021 gcaaagacat aaggtcttgc ttacaacagt tggtaaaatt ctcttcaacg atatcatgcc
    43081 agaggggcta ccatacttgc aagaaccaaa caatgccaac ttgacagaag gtgttccagc
    43141 taaatacttc ttgccacttg gtggagatat caaggaagct atcagcaatc ttgagctcaa
    43201 ccctccattc aagaagaaaa accttggaaa tatcatcgct gaaatcttca aacgtttccg
    43261 tacgacagaa acttctgccc tacttgaccg catgaagaac ctcggttacc accactcaac
    43321 tcttgcagga ttgacagtgg gtattgccga catcccagtc gttgatgaca aggctgaaat
    43381 cattgaagaa tcacacaaac gtgtcgaaca aatcaccaaa caattccgtc gtggtatgat
    43441 cacagacgac gagcgttaca atgctgttac agctgaatgg cgtgctgccc gtgaaaaact
    43501 tgagaaacgc ttgattgcta accaagatcc taagaaccca atcgttatga tgatggactc
    43561 tggagcccgt ggtaacatct caaacttctc acagcttgcc ggtatgcgtg gtctgatggc
    43621 tgctccgaat ggacgtatca tggaattgcc aatcctttca aacttccgcg aaggtttgtc
    43681 ggtattggaa atgttcttct caactcacgg tgctcgtaaa ggtatgaccg atacggccct
    43741 taagacagcc gactcaggtt acttgactcg tcgtttggtc gacgttgccc aagacgttat
    43801 catccgtgag gacgactgtg gaactgaccg tggtctcttg atccgttcta tcgcagaagg
    43861 aaaagagatg atcgagtctc tcgaagagcg tctcaacggt cgttacacta agaaaactgt
    43921 taaacatcca gaaactggtg cagtgattat tggtccaaat gaattgatta cagaagacaa
    43981 ggcgcgtgaa attgtcaatg ctggtgtgga agaagtgact atccgttctg tatttacatg
    44041 taacactcgt cacggtgtct gccgtcactg ttacggtatc aacttggcga ctggtgatgc
    44101 ggttgaagtt ggtgaagcag ttggtacaat cgctgcccaa tctatcgggg aacctggtac
    44161 acagcttaca atgcgtacct tccacacagg tggggttgcc tcaaataccg atatcactca
    44221 gggtcttcct cgtgtccaag aaatctttga agcccgcaat cctaaagggg aagcggttat
    44281 cacagaggtt aaaggacaag ttactgctat cgaagaggat gcatcaactc gtaccaagaa
    44341 agtctttgtt aagggtgaaa ctggcgaagg tgaatatgtc gttccattta cagctcgtat
    44401 gcgtgtcgaa gttgggggcc aagtagcgcg tggtgctgct ctgacagaag gttctatcca
    44461 accaaaacgt ctccttgcag ttcgtgatgt cttgtcagtt gaaacgtacc ttctcggtga
    44521 agtacaaaaa gtttaccgta gccaaggggt agaaatcggt gacaaacaca tcgaggtaat
    44581 ggttcgtcaa atgatccgta aagtccgtgt catggatcca ggtgatacag atcttctcat
    44641 gggtaccctc atggatatca atgactttac agatgccaac aaagatgtcc ttatcgcagg
    44701 tggagttcca gcgacaggtc gcccagtcct tatgggaatt accaaagcct cacttgaaac
    44761 aaacagtttc ttgtcagcgg cttccttcca ggaaacaact cgtgtcctta ctgacgcagc
    44821 tatccgtggt aagaaagacc atctccttgg acttaaagaa aatgttatca tcggtaagat
    44881 catcccagct ggtactggta tggcccgtta ccgtaacctt gaaccacatg ctgtcaacga
    44941 agaagaatac cttaatcctc cagtagagga agaaggaaat gaagaaacaa cagaagtagt
    45001 tgtggatact gccgttgaaa ctgtggaaga aacagtagaa taagagagaa tgagagaacc
    45061 cgagggttct ctcattctct cttctgaaaa ctttctccat ttttccacga aatgtggtaa
    45121 aataaaagaa agaagctgac aaaggagacg ggtatggaac aaacattctt tatcatcaaa
    45181 ccagatggtg taaaaagagg gctagtgggt gaagtgttaa agcgcatcga acaacgtgga
    45241 tttacaatcg aaaaattgga gtttcgttca caggtttcag aagagttgat tgaccagcac
    45301 tatcaggact tggttggtca gagtttttac ccaccgattc gtgaattcat gacttcaggt
    45361 ccagttcttg tgggtgtcat ttctggtccc aaagtaatcg aaacttggcg gaccatgatg
    45421 ggtgcaactc gtccagaaga agctttacca ggcactattc gaggtgattt tgcaaaagct
    45481 gctggagaaa atgagattat ccaaaatgtt gtacatggtt cagattcaga agagtcagct
    45541 aagcgagaaa ttgctctttg gttttaagag tggattggct caatcaattg gttaaaagct
    45601 catttgaata gaaagtatag tcaattagtt taagacatga cgcatgatat caaacttttt
    45661 agtttttgat atggtgcgtt ttttgattaa gtgactatta gtttgtctcg ctccgttagg
    45721 tgcgagacaa aaaaaacacc cgctatgcgg gtgcgcgtcg aaggttatac caaaaaaact
    45781 ccaaacgcga tacaataaag gtgttcaatc caattgtaaa gcgaaaggag aaaaatatgg
    45841 cacaaaaggt tcttttatcc tgttcttgtt tcaattgact atatttctat actttttctt
    45901 cctttctctg tgaaaatcgc aagtttttcc caccttttct caatactgaa acaacatctt
    45961 tccagaactt caataatttc tttttgtgtt aaatcattat ttaactaaat ctttggaggc
    46021 aatacgtttt caaagctact tttttaccat aggttttgag ccttcaacac agattactaa
    46081 gccaaaaatg ctctaaattt ttgaaattct aacaaattcc actcaacttt atcatcatct
    46141 ttgactttca caatatcttc ctcaaaataa catggaataa agatagtttt cttttaaaac
    46201 ggttgtagga ggaaaatctc tcctacacta ttattgattc tgagagaaaa ttgcaaaata
    46261 tcatctacca aactttcaat tattaagatt ttttgcagtt tatctgaatt agaatcgcaa
    46321 ggcttctcaa taatataatt gtcgaatttt ttcattctaa cttcctctcc attatttctt
    46381 cctttatcaa gaaaaagtta cctcaatatt aataaagtac aattctaaat taactccaat
    46441 tcttgattca tgataatatt tcagtaactc atcgtgcaat taaaaacaag gatttttatt
    46501 tcagatgatc catcgtgaac acttcatcag ccatctccca aattgccgga ttatgtgttg
    46561 cgataatgat tagcctatta tcatctcgaa gagatagcaa aatctccata atcaactgag
    46621 aggttgctgg gtctattgaa gctgttggct catctgccag aataaagggt ggattcttta
    46681 agataatttt tgccaaggca acccgttgcg attctccgcc cgataactca aagatgcgct
    46741 tatctaggtc aagataagcc aggccgacct gttctaaagc ctgcttctgc ctcaaccgct
    46801 gttccgaccg actcaacttt tgaccaatga gacctagctt aaggttttct tcaatacttt
    46861 ggttttcaat taagccaaag ttctggaaga ggtagcccaa ttcgtgacgg aaaaaatcac
    46921 tcgatttata attggccaag tctttacctc ggtaaaaaat cgtcccatca taaggttcta
    46981 atttcccaat catgttcatc aaggttgttt ttccgctacc acttgaacca attaaggcat
    47041 agacttttcc agcctcaaat gtcattgaaa gattcgaaaa taactctcgt tctccaaaag
    47101 atttactcac ctgtttaagt tcaatcatat taacctccct tcaaaacaag aatggacatc
    47161 ttgttttctt tctgcatttg gacatgtaac tgtaaaagag atagaccagt aaagagtaac
    47221 aagactaaga aagcaactcc tatctctacc tgaagaaata cactcgcaac aaatcccagt
    47281 aaaaacacac ccagttgagc aaagagataa gtgcgatgga tttctaagaa cctgagacct
    47341 gcaatgcgtt tgataaaaat ggcacgtcta aattcttcaa agtagagcct attcatagtg
    47401 ttaaacaaca agattgaagt tgcaatccca agcacagctc ctgctagcat tacccaacgt
    47461 tccctctgga tattatccaa taatgtgatg tagttgtggt aacctgtttg catttctgag
    47521 acccaatttt caatgccttg tctctggata agctcctggg catcagacaa ttgattaaag
    47581 agaacgtagt tctgtactgc gtctatccaa aacaaaatgg actgtggacc agttgattgg
    47641 ggtgttataa caatgatgat tggatctttc aaaaactgct ggtaagaaat aggggtcgta
    47701 ttatacacaa atcgatcctg acctgattct aaataaccta ccgtagcagt catttgctgt
    47761 cgttcatctt gactagatat gcgactggtt aagtcgtctt caaaaacaga tttataatgt
    47821 tcttcctctg aacggaggtg ttcaggtagc aataagacaa actcccctac attaaggtga
    47881 ttcatctttt gctcaatagt tgtatctaca ggaatgtttt gacgctccaa gtattgcggt
    47941 gtgacgataa ggacattgcc atttgggttg taatcgtgcc attctgtaga ggttatcaag
    48001 tttttggagg aagccatgcc attttgcaaa gtacggtcaa ttaactggtg tctagataag
    48061 aaagcctttt gttctgaaac agccagatcc atcaattgat accaagttct gagtttcctt
    48121 tgagcttgtt catcaaaacc aggactcgtt ccttcacggc tgattgatag ggtaatcagt
    48181 tgcctttcct gactccaaat ctcttgtcca atccgatgct gttgccaggc ttgggaatac
    48241 tttaagctac tccctattcc cagcgttaca ataataatcg ctaatagttg accgattaaa
    48301 atcaaactaa tgattcctct gacaggcact tgccctttta taatctgcat caggtgtatc
    48361 ttttttatcc caactgcgaa gagttgagcg aaaaacaggg agatcgacaa caagatgaga
    48421 ttataactga gcaagccttc tcctatggtc attagggatt gcgttggaag cgacagaatt
    48481 ttttgcataa gaatggcgag cacgccagct aaactgaaac caacagctat cgctttcaaa
    48541 tcctcaccaa ctggtctaag gaaaatggac caccgtttct ctcctgaaat gaggcgaatg
    48601 cccgatgacc gtaattggct aatttgacta attaaagtca gtgctacaaa ggttaagatg
    48661 aaaatcaata gactgattaa ctgaaatccg ttactaaaga tagccattaa cgtagatagt
    48721 ttagatggaa ttgtcaggtg catgttagtc aagccaagtt gacttagctc ttcccttagc
    48781 aagtgaatat ctaggtgtcc atcgaataca aaatagtttg tttcaacact actactttga
    48841 gcatcttcta gatttttttc ctgaagccca tcaggcaact ttccgtcccc aaaggtcgta
    48901 taagaaaaaa ctgtagtacc ttctgagtta ggatcttggt gttgaatcgc aataaagcta
    48961 tctgtttctt ctgctagttt ttccaagcgt gtagcaacat gctgaaaagt tgtttcaggg
    49021 gaagaatcac gtacaacaac gttggggtaa taatgggaga catatgtatc agtccaaatg
    49081 gtaagtaccc aaacaaagaa caaactagca agcaggttgg atatgagtat aaataatttt
    49141 ttcatagtgc attccttatt ctaaaacaga gggcagaaat acccctgccc tctataaact
    49201 aacaagctta ctaaactatt gataaaagaa tcttcttcaa ttattcttta ccatcccgca
    49261 tcaaatctaa attttaacat attattactc ctctgaatga aatattatga aaatgagcga
    49321 aatgcaacta catattttaa ttagaaatct cagtctgaat tatagtatcc tattttttcc
    49381 tcctttctaa ctgcacgttc ctttctacag gcagagacct aattttataa accaatctta
    49441 aatactttag attttgtaac tcataatttt caacagttgg ttagctatga aagcatttta
    49501 tcaaacatct ccattcttat atctatctgg tattgttaca agctctcatc agaatacctt
    49561 tctctaaata gttgaatttt aaagtaatac gaaatcaatc agccttccca acatcaagac
    49621 cattggactt cttttgtgat tgcactcgaa tctccaattg tctattcttc tgaataaact
    49681 gttggtaagg aattgtgtta ctaaacataa aaacacctag taatactaat atagctaatt
    49741 tttgaattcc acgatttttc ttcattcgtg aaaccctgtt ctttttcctt gttt
//
LOCUS       DAVKAO010000007        46070 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKAO010000007 DAVKAO010000000
VERSION     DAVKAO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.007"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(540..632)
                     /locus_tag="VWX16_000430"
     CDS             complement(540..632)
                     /locus_tag="VWX16_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9157842.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            793..1764
                     /gene="ruvB"
                     /locus_tag="VWX16_000431"
     CDS             793..1764
                     /gene="ruvB"
                     /locus_tag="VWX16_000431"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9157843.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1745..2317
                     /locus_tag="VWX16_000432"
                     /pseudo
     CDS             1745..2317
                     /locus_tag="VWX16_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            2548..3306
                     /locus_tag="VWX16_000433"
     CDS             2548..3306
                     /locus_tag="VWX16_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9157844.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3315..4118
                     /locus_tag="VWX16_000434"
     CDS             3315..4118
                     /locus_tag="VWX16_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9157845.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFVLTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4140..5399
                     /gene="rseP"
                     /locus_tag="VWX16_000435"
     CDS             4140..5399
                     /gene="rseP"
                     /locus_tag="VWX16_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9157846.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5412..7265
                     /locus_tag="VWX16_000436"
     CDS             5412..7265
                     /locus_tag="VWX16_000436"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9157847.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVETPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7365..8744
                     /gene="bglA"
                     /locus_tag="VWX16_000437"
     CDS             7365..8744
                     /gene="bglA"
                     /locus_tag="VWX16_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9157848.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            8937..10745
                     /gene="glmS"
                     /locus_tag="VWX16_000438"
     CDS             8937..10745
                     /gene="glmS"
                     /locus_tag="VWX16_000438"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9157849.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIVEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            10864..11913
                     /locus_tag="VWX16_000439"
     CDS             10864..11913
                     /locus_tag="VWX16_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9157850.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGIPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHVDKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(12013..15876)
                     /gene="spuA"
                     /locus_tag="VWX16_000440"
     CDS             complement(12013..15876)
                     /gene="spuA"
                     /locus_tag="VWX16_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9157851.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPATEPP
                     TPAASPADPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQ
                     GLWTWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNL
                     TGDKSVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVK
                     NPSSAQWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFT
                     DLKNHSQIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKH
                     SNITNHLGNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDE
                     TYSYDGKLGSDLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTW
                     KQTLDSTNKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVA
                     KAAFVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEA
                     FIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSL
                     TGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFM
                     DADGTPRTSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEA
                     YKAARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGY
                     PNEGQPAFITGGKRDINTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSI
                     KKDPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYRTPVAEDK
                     VPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKG
                     LIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVN
                     ADEKAREFNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLR
                     VSQNGTSHESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENK
                     PSQATADSQAEQPAQEAQASSVKEAVRKESVENSSKENISATPDRQAELPNTGIKNEN
                     KLLFAGISLLALLGLGFLLKNKKEN"
     gene            16293..16706
                     /gene="rpsL"
                     /locus_tag="VWX16_000441"
     CDS             16293..16706
                     /gene="rpsL"
                     /locus_tag="VWX16_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9157852.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16726..17196
                     /gene="rpsG"
                     /locus_tag="VWX16_000442"
     CDS             16726..17196
                     /gene="rpsG"
                     /locus_tag="VWX16_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9157853.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17621..19702
                     /gene="fusA"
                     /locus_tag="VWX16_000443"
     CDS             17621..19702
                     /gene="fusA"
                     /locus_tag="VWX16_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9157854.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19806..24197
                     /locus_tag="VWX16_000444"
     CDS             19806..24197
                     /locus_tag="VWX16_000444"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9157855.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDVQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQTTEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24299..24562
                     /locus_tag="VWX16_000445"
     CDS             24299..24562
                     /locus_tag="VWX16_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9157856.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24555..24833
                     /locus_tag="VWX16_000446"
     CDS             24555..24833
                     /locus_tag="VWX16_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9157857.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVCECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24854..25006
                     /locus_tag="VWX16_000447"
     CDS             24854..25006
                     /locus_tag="VWX16_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157858.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            25028..26269
                     /locus_tag="VWX16_000448"
     CDS             25028..26269
                     /locus_tag="VWX16_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9157859.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NATSDEEAVDLLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVLLFRNGNWAN"
     gene            26279..26509
                     /locus_tag="VWX16_000449"
     CDS             26279..26509
                     /locus_tag="VWX16_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9157860.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            26732..27454
                     /locus_tag="VWX16_000450"
     CDS             26732..27454
                     /locus_tag="VWX16_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9157861.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            27672..29006
                     /gene="pepC"
                     /locus_tag="VWX16_000451"
     CDS             27672..29006
                     /gene="pepC"
                     /locus_tag="VWX16_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9157862.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAETIVLAPWDPMGALAE"
     gene            complement(29062..29973)
                     /locus_tag="VWX16_000452"
     CDS             complement(29062..29973)
                     /locus_tag="VWX16_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9157863.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(29997..30800)
                     /locus_tag="VWX16_000453"
     CDS             complement(29997..30800)
                     /locus_tag="VWX16_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9157864.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(30828..31817)
                     /gene="manX"
                     /locus_tag="VWX16_000454"
     CDS             complement(30828..31817)
                     /gene="manX"
                     /locus_tag="VWX16_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9157865.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVVANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQAAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIDISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(32050..33069)
                     /gene="adhP"
                     /locus_tag="VWX16_000455"
     CDS             complement(32050..33069)
                     /gene="adhP"
                     /locus_tag="VWX16_000455"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9157866.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVARLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(33408..34220)
                     /locus_tag="VWX16_000456"
     CDS             complement(33408..34220)
                     /locus_tag="VWX16_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9157867.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKNPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            34410..35828
                     /locus_tag="VWX16_000457"
     CDS             34410..35828
                     /locus_tag="VWX16_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9157868.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            35882..36616
                     /locus_tag="VWX16_000458"
     CDS             35882..36616
                     /locus_tag="VWX16_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9157869.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQMSNETTLLQMSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIV
                     PKKIFRGKESWGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILL
                     HMIVNGIAFCLLALVVIMSRTLGISV"
     gene            36710..37684
                     /gene="folP"
                     /locus_tag="VWX16_000459"
     CDS             36710..37684
                     /gene="folP"
                     /locus_tag="VWX16_000459"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9157870.1"
                     /translation="MMKSLEGVTDMSSKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLITEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFNQTFTVEELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            37686..39008
                     /locus_tag="VWX16_000460"
     CDS             37686..39008
                     /locus_tag="VWX16_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9157871.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQDSVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNVTDKKEEKQTVRIVTGSLYFLSQVRVYLMERKNENGYTKD"
     gene            38989..39543
                     /gene="folE"
                     /locus_tag="VWX16_000461"
     CDS             38989..39543
                     /gene="folE"
                     /locus_tag="VWX16_000461"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9157872.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVIIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            39586..40398
                     /gene="folK"
                     /locus_tag="VWX16_000462"
     CDS             39586..40398
                     /gene="folK"
                     /locus_tag="VWX16_000462"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9157873.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFEFYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(40443..40760)
                     /locus_tag="VWX16_000463"
     CDS             complement(40443..40760)
                     /locus_tag="VWX16_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9157874.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            41115..41561
                     /gene="rplM"
                     /locus_tag="VWX16_000464"
     CDS             41115..41561
                     /gene="rplM"
                     /locus_tag="VWX16_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9157875.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            41580..41972
                     /gene="rpsI"
                     /locus_tag="VWX16_000465"
     CDS             41580..41972
                     /gene="rpsI"
                     /locus_tag="VWX16_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9157876.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            42363..42701
                     /locus_tag="VWX16_000466"
     CDS             42363..42701
                     /locus_tag="VWX16_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU9157877.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDKHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            42891..44327
                     /locus_tag="VWX16_000467"
     CDS             42891..44327
                     /locus_tag="VWX16_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9157878.1"
                     /translation="MTTFKDRFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSLAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAVHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            44379..44618
                     /locus_tag="VWX16_000468"
     CDS             44379..44618
                     /locus_tag="VWX16_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU9157879.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGTIALGMYVMQVIGIAL
                     WLAHGICIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            44748..45062
                     /locus_tag="VWX16_000469"
     CDS             44748..45062
                     /locus_tag="VWX16_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU9157880.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            45180..>46070
                     /locus_tag="VWX16_000470"
     CDS             45180..>46070
                     /locus_tag="VWX16_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9157881.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKFGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKN"
BASE COUNT        13339 a         8620 c        10574 g        13537 t
ORIGIN      
        1 aatcctaaac ttttcttttt cataataatc tcccttaact ccacccaatc aggtggagtt
       61 ttttagctct atttcaggct tttggggact attctaaaaa taatttttcg atatttttcg
      121 gtatttttcg gattttggtc ggggaattgg cggggacttt tttagcgaat ataactaaga
      181 aataggtctg tggtcgcttc agctagttca aactcaattt gattgtaagt gactgttaat
      241 tgaagaactg gaactactgt aataatgatt ttgcttgtct agtagtggcg tattaaatga
      301 gaagtgacat gtaagattgt ctcttcgttt acgaaatcaa aatttctatg aaattgatag
      361 aatccctaac gttctcttaa tcttaaccgt cttgtcgtac agcggttgaa gaaaatgtaa
      421 acctttgaca aaattcgtga actatgggat aataaaaagg aattgagtac tagttctata
      481 tcataggcta gaaaagaccc caagctcacg accaaataag cttgaggtct tttttgacac
      541 tatttgtcct tgtttagcca tttatcgact atacgaagaa cgacaccgac cacaattggt
      601 ccgatgatag ttttgagtac taattccatc atgggctatc tcacctcctt tcgtaggcgg
      661 tgtagcagtg tcgtagaata ttataccaca taagtgctaa acttaggagt cacccaatca
      721 ggtggctttt ttgtttgtgg cttggttttt tgatataata gagccatgag tagaatttta
      781 gataatgaga taatggggga tgaggagtta gtagaacgca cgctccgtcc tcagtattta
      841 cgtgaatata tcggacagga taaggtcaag gaccagctac aaatctttat tgaagctgcc
      901 aaaatgcggg atgaagcgct ggatcatgtg ctcttatttg ggcctccagg tctcgggaaa
      961 acgaccatgg cctttgttat tgccaacgaa ctgggagtca atcttaagca gacgtcgggt
     1021 ccagtcattg aaaaagccgg agatctggta gctattttga atgagttaga gcctggggat
     1081 gtacttttta ttgatgagat ccatcgtttg ccaatgtcag tggaagaggt gctttatagt
     1141 gctatggagg acttctacat agatattatg attggggctg gtgagggtag tcgtagtgtt
     1201 catttggagt taccaccttt taccttgatt ggtgcgacga ctcgggctgg tatgctctcc
     1261 aatccgctac gggcacgttt tgggattaca ggccatatgg agtattatgc ccatgctgac
     1321 ttgacagaaa ttgtcgagcg gacggcagat atttttgaga tggaaatcac tcatgaggca
     1381 gcatctgagt tggccctacg tagtcgtggg acccctcgta ttgccaatcg tctcctcaag
     1441 cgcgtgcgcg attttgccca gataatgggg aatggggtaa ttgatgatat tattaccgat
     1501 aaggctttga ctatgctgga tgttgaccat gaaggtttgg actatgtgga tcaaaaaatc
     1561 cttcgtacca tgattgagat gtacagtgga ggacctgttg gtctaggaac tctttctgtg
     1621 aatatcgccg aagagcgtga gacagttgaa gatatgtatg agccttactt gattcaaaaa
     1681 ggttttatca tgcggacacg gtctggacgg gtggcgactg ctaaggcata tgagcactta
     1741 ggttatgaat acagtgaaaa ataagcaaga aatcttagag gcttttagag aaaatccaga
     1801 tatgatggcc attctgacga tcatccgaga ccttggtctg aaagactcgt ggttggcagc
     1861 aggttctgtc agaaatttca tttggaatct tttgtcagac aaatcccctt ttgatcatga
     1921 aacagatata gatgtgattt tctttgatcc agatttttct tatgaggaaa ccttattact
     1981 ggagaaaaag ctgtgagagg attttcctca gtaccagtgg gaattgaaaa atcaggtcta
     2041 tatgcatcag cacagtcctc acactgcttc ctataccagt tctcgtgatg ctatgagtaa
     2101 gtatccagaa cggtgtacga cagttggact gcgcttgaat gaagaatcag attttgaact
     2161 ctatgcacct tatggtttgg aggatatttt gaattttcaa gttcgtccaa ctcctcattt
     2221 cttagaaaat gaagaccgaa tggaactcta tcaaacacgt ttatccaaga aaaattggca
     2281 ggagaaatgg aaaaatttga tttttaaaaa tacttaagga aactttaagc tagggagtgt
     2341 acactaagtt cataagttaa gaagacctta acttaaactc ctaaaacttt ttcataataa
     2401 tctccctata aaaataaagt cgcccaatca ggcggcttat ttttttgaaa aatgggcttg
     2461 gtgcctgaga ataaatagct tagtgataga agaaaatggg gaaatatggt ataatgaaac
     2521 gatagatttt tgaataggaa taagatcatg tttggatttt ttaagaaaga taaggctgtg
     2581 gaagtagagg ttccgacaca ggttcctgct catatcggca tcatcatgga tggcaatggc
     2641 cgttgggcta aaaaacgtat gcaaccgcga gtttttggac ataaggcggg catggaagca
     2701 ttgcaaaccg tgaccaaggc agccaacaaa ctgggcgtca aggttattac ggtctatgct
     2761 ttttctacgg aaaactggac ccgtccagat caggaagtca agtttatcat gaacttgcca
     2821 gtagagtttt atgataatta tgtcccggaa ctacatgcga ataatgttaa gattcaaatg
     2881 attggggaga cagaccgcct gcctaagcaa accttcgaag ctttaaccaa ggctgaggaa
     2941 ttgactaaga acaacacagg attgattctt aattttgctc ttaactatgg tggacgtgct
     3001 gagattacac aggcgcttaa gttgatttcc caggatgttt tagatgccaa aatcaaccca
     3061 ggtgacatca cagaggaatt gattggtaac tatctcttta cccagcattt gcctaaggac
     3121 ttacgagacc cagacttgat tatccgtact agtggagaat tgcgtttgag caatttcctt
     3181 ccatggcagg gagcctatag tgagctttat tttacggaca ccttatggcc tgattttgac
     3241 gaagcggcct tgcaggaagc tattcttgcc tataatcgtc gccatcgccg atttggagga
     3301 gtttaggagg aaatatgacc caggatttac agaaaagaac cttgtttgca gggattgccc
     3361 tggctatttt cctaccaatt ttaatgattg ggggcctctt gcttcagata gcaatcggaa
     3421 tcatagccat gctagccatg catgaacttt tgaagatgag aggtctagag accatgacga
     3481 tggagggcct cttgaccctc tttgcaacct ttgtattgac cattcccctg gaaaattacc
     3541 tgactttttt gccagttgat gggaatgtgg ttgcctatag tgttttgatt tcaatcatgt
     3601 taggaacgac cgtttttagc aagtcttata cgattgagga tgcggttttc cctcttgcta
     3661 tgagcttcta cgtgggcttt ggatttaatg ctttactaga tgctcgtgtt gcaggtttgg
     3721 acaaggctct cttagccttg tgtatcgtct gggcgacaga cagtggtgcc tatcttgttg
     3781 ggatgaacta tgggaaacga aagttagcac caagggtatc gcctaataaa acccttgagg
     3841 gtgccttggg tggtatttta ggagcaattt tagtaaccat tatctttatg atagttgaca
     3901 gtacagttgc tcttccatat ggaatttaca agatgtcagt ctttgctatt ttctttagca
     3961 ttgctggaca atttggtgat ttactagaaa gttcgatcaa acgtcatttt ggtgttaagg
     4021 attctgggaa atttatccct ggacatggtg gtgttttgga tcgtttcgat agtatgttgc
     4081 ttgtatttcc aatcatgcac ttatttggac tcttttaatc aaaagacgga ggaaacgcta
     4141 tgctcggaat tttaaccttt attctggttt ttgggattat tgtagtggtg cacgagttcg
     4201 ggcacttcta ctttgccaag aaatcaggga ttttagtacg tgaatttgcc atcggtatgg
     4261 gacctaaaat ctttgctcac attggcaagg atggaacggc ctataccatt cgaatcttgc
     4321 ctctgggtgg ctatgtccgc atggccggtt ggggtgatga tacaactgaa atcaagacag
     4381 gaacgcctgt tagtttgaca cttgctgatg atggtaaggt taaacgcatc aatctctcag
     4441 gtaaaaaatt ggatcaaaca gccctcccta tgcaggtgac ccagtttgat tttgaagaca
     4501 agctctttat caaaggattg gttctggaag aagaaaaaac atttgcagtg gatcacgatg
     4561 caacggttgt ggaagcagat ggtactgagg ttcggattgc acctttagat gttcaatatc
     4621 aaaatgcgac tatctggggc aaactgatta ccaattttgc aggtcctatg aacaatttta
     4681 tcttaggtgt tgttgttttt tgggttttaa tctttatgca gggtggtgtc agagatgttg
     4741 ataccaatca gttccatatc atgccccaag gtgccttggc caaggtagga gtaccagaaa
     4801 cggcacaaat taccaagatt ggctcacatg aggttagcaa ctgggaaagc ttgatccaag
     4861 ctgtggaaac agaaaccaaa gataagacgg caccgacttt ggatgtgact atttctgaaa
     4921 aggggagtga caaacaagtc actgttacac ccgaagatag tcaaggtcgt taccttctag
     4981 gtgttcaacc gggggttaag tcagattttc tatccatgtt tgtaggtggt tttacaactg
     5041 ctgctgactc agctctccga attctctcag ctctgaaaaa tctgattttc caaccggatt
     5101 tgaacaagtt gggtggacct gttgctatct ttaaggcaag tagtgatgct gctaaaaatg
     5161 gaattgagaa tatcttgtac ttcttggcaa tgatttccat caatattggg atttttaatc
     5221 ttattccgat tccagccttg gatggtggta agattgtgct caatatccta gaagccatcc
     5281 gccgcaaacc attgaaacaa gaaattgaaa cctatgtcac cttggccgga gtggtcatca
     5341 tggttgtctt gatgattgct gtgacttgga atgacattat gcgactcttt tttagataat
     5401 cgaggaatat tatgaaacaa agtaaaatgc ctatcccaac gcttcgcgaa atgccaagcg
     5461 atgctcaagt tatcagccat gctcttatgt tgcgtgctgg ttatgttcgc caagtttcag
     5521 caggtgttta ttcttatcta ccacttgcca accgtgtgat tgaaaaagct aaaaacatca
     5581 tgcgccaaga attcgaaaag attggtgctg ttgagatgtt ggctccagcc cttcttagtg
     5641 cagaattgtg gcgtgaatca ggtcgttacg aaacctatgg tgaagacctt tacaaactga
     5701 aaaaccgtga aaaatcagac tttatcttag gtccaactca cgaagaaacc tttacagcta
     5761 ttgtccgtga ttctgttaaa tcttacaagc aattgccact caacctttat caaattcagc
     5821 ccaagtatcg tgatgaaaaa cgcccacgta atggacttct tcgtacacgt gagtttatca
     5881 tgaaggatgc ttatagtttc cacgctaact atgatagttt ggatagtgtt tatgatgagt
     5941 acaaagcagc ctatgagcgt attttcactc gtagtggttt agacttcaag gctattattg
     6001 gtgacggtgg agccatgggt ggtaaggata gccaagaatt tatggccatt acatctgctc
     6061 gtacagacct tgaccgctgg gttgtcttgg acaagtcagt tgcctcattt gacgaaattc
     6121 ctgcagaagt gcaagaagaa atcaaggcag aattgctcaa atggatagtc tctggtgaag
     6181 ataccattgc ttactcaagt gagtctagct atgcagctaa cttagaaatg gcaacaaacg
     6241 agtacaaacc aagcaaccgt gttgtcgctg aagaagaagt tactcgtgtt gaaacgccag
     6301 atgttaaatc aattgatgaa gttgcagcct tcctcaatgt tccagaagaa caaacgatta
     6361 aaaccctctt ctacattgca gatggtgagc ttgttgcagc ccttctagtt ggaaatgacc
     6421 aactcaacga agtcaagttg aaaaatcact tgggagcaga tttctttgac gttgctagcg
     6481 aagaagaagt ggcgaatgtt gttcaagcag gatttggttc acttggacca gttggtttgc
     6541 cagagaatat taaaattatt gcagatcgta aggtgcaaga tgttcgcaat gcagttgtcg
     6601 gtgctaacga agatggctac cacttgactg gtgtgaaccc aggccgtgat tttactgcag
     6661 aatatgtgga tatccgtgaa gttcgtgagg gtgaaatttc cccagatgga caaggtgtcc
     6721 ttaactttgc gcgtggtatt gagatcggtc atattttcaa actcggaact cgctattcag
     6781 caagcatggg agcagatgtc ttggatgaaa atggtcgtgc tgtgccaatc atcatgggat
     6841 gttacggtat cggtgtcagc cgtcttcttt cagcagtgat ggagcaacac gctcgcctct
     6901 ttgttaacaa aacgccaaaa ggtgaatacc gttacgcttg gggaatcaat ttccctaaag
     6961 aattggcacc atttgatgtg catttgatta ctgttaatgt caaggatgaa gaagcgcaag
     7021 ccttgacaga aaaacttgaa gcaagcttga tgggagctgg ttatgaagtc ttgacagatg
     7081 accgtaacga acgtgtcggt gttaaattta gcgatagtga cttgattggt cttccaatcc
     7141 gtatcactgt tggtaaaaaa gcggccgatg gcattgtaga agttaagatc aaggcaactg
     7201 gtgacaccat tgaggttcat gcagataacg tgcttgaaac gcttgaaatc ctaagcaaga
     7261 aataaaaact ataatcagaa gaaaaacaag gaaaaaatgt aactagtttt taccttgttt
     7321 tttctgtata atgggagaaa tgagtagata aagaggtaaa tgttatgcta agatttccaa
     7381 aggattttgt ctggggatcc tctacttctg gaccgcagac agaaggacgt gtagctggtg
     7441 acggtaaggg agacaatctc tgggattact ggttccaagt ggagccaaat cgttactata
     7501 atgggattgg ttcagataag acatcgactt tttatgaaaa ttgggagcgg gatattgagc
     7561 ttttgttaga gactggtcac acagtctttc gaacttctat tcagtggtca cggatttttc
     7621 cacaaggctg tggaaaagtc aaccctcaag gtgtggattt ttatcgtaag gtctttgagg
     7681 ctattaaggc taaaggaatt cgtctgttag tcaatctcta tcattttgat ttaccttttg
     7741 cccttcaaga ggatggtgat ggttgggaaa ataaggcgac agtctcagcc tatgaagact
     7801 atgctcgttt ttgttttgag acttatggag atttagtgga tcaatggatt acctttaacg
     7861 agcccatcgt tcctgtagaa tttggttatt tttatgatgc ccattatcca cataaggtgg
     7921 atgcagaggc agctgttaag gtagcctatc atacacaatt ggccagcagc cgggctgtta
     7981 aggcttgcca tgaacttttg cctgattcca agattgggat tgtcctcaac ttgacaccgg
     8041 cttatccacg tagccagcat cctgctgatg tcaaggcagc tcgtattgcg gccctttttc
     8101 aggcccaatc tttcttagat ccatctgtct tggggactta tccacaggag ttggtagaaa
     8161 tcttgcatga acacggtctt ttacctgatg ctacagagga agagttggaa ctcattcgtg
     8221 ataatacggt ggacttcctt ggtgtgaact actatcaacc tttgcgtgtt atggctcctc
     8281 gatttgctaa gcatccagag agtccactct taccagaaca tttttacgag ccttatgtga
     8341 tgcctggacg taaaatcaat tctcaccgtg gttgggagat ttatgagcaa gggatttatg
     8401 acatcgccca aaatatcaag gaaaattatg gcaatattga atggatgttg actgagaatg
     8461 gtatgggtgt tgaaggggaa gaaaaattcc gtcaagatgg aatgattcaa gatgattacc
     8521 gtattgactt tgtaaaaggt catcttcgtg aacttcaccg tgccattgaa gatggtgcca
     8581 actgtaaggg ctacttgatt tggaccttta ttgactgctg gtcatggctc aacagctata
     8641 aaaatcgcta tggtttggtc gaattagact tggaaacgca agaacgtcgt ctgaagaaat
     8701 caggccactg gttcaaagaa ttaagcgata ataatggatt ttaaaaagga ctctgactga
     8761 ttatcccaat cggtcagagt ttttatgctt acaggagctt aatttgaact ataccaattt
     8821 ttactcttga caagtgaaag aaacaagtat atactgtttt tgctgattct ataaaagatg
     8881 aattagacta taccaatttt aaggagaaag cacagcttgc ctgtgtcgta tatactatgt
     8941 gtggaattgt tggtgttgtt ggaaacacaa atgcaactga tattttgatt caagggcttg
     9001 aaaagcttga ataccgtggc tatgattctg cgggaatttt tgtcctagat ggtgctgata
     9061 accatttggt gaaggcggtt ggtcgtattg cagaattgtc tgccaagaca gccggtgttg
     9121 agggaacaac tggtatcgga catactcgtt gggctactca cggaaaacca actgaggaca
     9181 atgctcaccc acaccgctct gagacagaac gttttgtctt ggtgcataat ggggtgattg
     9241 agaactatct tgaaatcaag gaagaatacc ttgcaggtca ccacttcaag gggcagacag
     9301 atactgaaat agccgttcat ttgattggaa aatttgcgga agaagaaggg ctctcagttc
     9361 ttgaagcctt caaaaaagcc cttcacatca tccgtggttc ttatgccttt gccttggttg
     9421 actcacaaga tcctgaagtc atctacgtag ctaaaaataa atcaccactt ttgattggtc
     9481 ttggagaagg ctataacatg gtctgctcag acgccatggc tatgattcgt gaaactaacc
     9541 aatacatgga aatccatgac caagagttgg taatcgtcaa ggctgatagc gtggaagttc
     9601 aagactatga tggtaacagt cgtgaacgtg ctagctatac tgcggaactt gacttgtcag
     9661 atatcggtaa gggaacttat ccttactaca tgcttaagga aattgatgag caaccaactg
     9721 ttatgcgtaa actcattcaa gcctacacgg atgatgctgg tcaagtagtg gttgatcctg
     9781 ctatcattaa ggctgttcaa gacgcagacc gcatctacat ccttgcagct ggaacatctt
     9841 accatgcagg atttgcttct aagaaaatgt tggaagaatt gacagataca ccagttgaac
     9901 ttggaatttc atctgagtgg ggctacggta tgccacttct cagcaagaaa ccactcttca
     9961 tctttatcag ccaatctggt gaaacagcgg atagtcgtca agttttggtc aaggctaatg
    10021 aaatgggaat tccaagctta acagtgacaa atgttccagg ttcaaccctc tcacgtgaag
    10081 ccaactatac catgctcctt cacgcaggtc ctgaaattgc cgtggcatca actaaagcct
    10141 atacagcgca aatcgcagcc cttgccttcc ttgcaaaagc agtcggagaa gcaaatggta
    10201 atgctaaagc gcaagccttt gacctggttc atgaattgtc aatcgtagct cagtctatcg
    10261 aatcaactct ttcagagaaa gaaaccattg aagtcaaggt tcgtgaactt cttgaaacaa
    10321 ctcgtaacgc cttttacatc ggacgtggtc aagattacta cgtagccatg gaagcaagtc
    10381 tcaaactcaa agagatttct tatatccagt gtgaaggttt tgcggcagga gaactcaagc
    10441 acggaaccat tgccttgatt gaagaaggaa cgcctgtttt ggctctcttg tcagatccag
    10501 ttcttgccaa ccatactcgt ggaaatatcc aagaggtcgc agcccgtggt gccaaagtcc
    10561 tcactatcgt agaagagaat gtagccaaag ataccgacga tatcgtcctt acgaccgtac
    10621 atccatacct ctcaccaatt tcaatggtcg taccaacgca attagtcgct tactttgcaa
    10681 ccctccaccg tggcctcgat gtggataaac cacgtaacct tgctaaatca gtaacggtag
    10741 aataagctaa aaaagtctag ttatttaggc tttttctaat gagcaaattg gttgcttgta
    10801 cttaagattc gtgttagaat aatttttcaa attgaaggac agagatagac aaggagaatc
    10861 acaatggtag aattgggaat ttcaacattt ggggaaataa cggagcttga agggactgga
    10921 caaacttaca gtcatgctga acgcattcgc cagttggtgg cagagattga gctggctgac
    10981 aaggttggtt tggacgtata tgggattggc gagcaccatc gggcggattt tgcagtatca
    11041 gccccagaga ttgtcctggc cgctggggca gtcaatacca agaaaatccg tttgaccagc
    11101 gcagtcagca ttctgtcaag catggacccg attcgtttgt tccaacagta tgccactatc
    11161 gatgctttgt caaatggacg agcggagatt atggctggaa ggggctcttt cacggaatct
    11221 ttccctctgt ttggttatga cttgaaagac tacgactctc tttttgatga gaaattagac
    11281 ttgcttcagt tagtcaatga aaagaccaag ttagactggc aaggtcgatt gactcaaacg
    11341 attgctggta aagaagttta tcctcgtcca gttcaggaca aattgccctt gtggatagct
    11401 acaggtggtc atgtcgaatc aacagtgaag attgctcagg ctggtatacc gattgtatat
    11461 gctattattg gtggtaatcc acgttatttt aaaaagttga ttcaggctta tcgtgagatt
    11521 ggaagcgaag cgggccatgt tgacaaagac ttgaaagtgg gagcccattc ttgggggtgg
    11581 attgcggaag atggtgagca ggcggtgaaa gattatttcc atccgactaa gcaagtggtg
    11641 gatgctattt ccaaagaccg tccgcactgg caggaattgc gttatgagca atatttggag
    11701 caggtagggc caaatggcgc catgtttgtt ggcaatccag atcaggtggc cgaaaaattg
    11761 attcgcatga tagaggattt aggattggac cgcttcatgc ttcatctacc gcttggttct
    11821 atgcctcatg accaggttct gagagctatt gaactcttcg gaacacaagt tgcacccaaa
    11881 gtacgagctt attttgctat gaaagaggct taataaaaaa tcctaatcaa gtttattagg
    11941 acaacaaata ttgtacgaaa attaatcccc cgagctataa tgcttggggg atttttctat
    12001 aggagggcta gcttagttct cttttttatt ttttagtaag aaaccgagac caaggagcgc
    12061 aaggaggctg attcctgcaa ataggagttt gttttcgttt ttgattcctg tatttggaag
    12121 ttcagcttgt ctatctgggg ttgcagatat attttccttg ctagagtttt ctaccgattc
    12181 ttttcgaacc gcttctttta cagatgatgc ttgtgcttct tgtgctggtt gttcagcttg
    12241 tgaatcagct gtagcttggc taggtttatt ttccgcatca gctactttgg catctggttt
    12301 agtagaatca ggtctagctt ctggagctgg gtcttgatgt gcagggtgag aagcttcatt
    12361 ttgatgttca ggcttggaag gggttgagtc tggtttctct tctgcagttg actcatggct
    12421 agttccattt tgagagactc gaagaacagt agctgtaagg gcattcaatt tcaagccttt
    12481 ttcagtccat tcaagtcctt tcgggttggc aattccgact gatcctgctt ggttttcatc
    12541 tgccaaaact tccgcatttc ttagatgtgc aaaggcagtt cccaaattaa attcgcgagc
    12601 tttttcatcc gcattgacaa agactgcgta gatatcgccg tttggagcag tgatttgata
    12661 gccaatcact acatcctctt tttccacacc attttggcct gggacagtga tgaggtggac
    12721 acggtctttg atatcttgaa gactcttaag tcggaaggca tctgtagatt gacgaagggc
    12781 aatcaaacct ttcatatagt cacggctctt gacattttca ggataagctt taccatctgt
    12841 agccttagtc cagtcaaact tgttgactgc atcactagaa tcgtaagagt catggatgaa
    12901 gtaaggatag tcaaatgggt tgccgtcctt atcacgcaac aagtgagatt tgtttggaac
    12961 cttatcctct gctacaggag ttctgtaggc tgggtcacgg aattgtttgg tacgtccata
    13021 ttcctggcca gagtggataa acggagttcc ttgagctgtc aagaccatga gatttccaag
    13081 tcgtaaacga cggtggattt cagcatagtt ctcagctttg cttgggtctt ttttgataga
    13141 ctgggcaatg atgtcaaaga gggtcaagtt atcatgggct gcgatgtatt ggatgacatc
    13201 tccaggactg tctgcttcaa agttggttgg ttgagcaatg aggtttttga agatagtgtt
    13261 gatatcacgc ttgccacctg tgataaaggc aggttgacct tcgtttggat aaccagactt
    13321 gaggttgtta cggatgtcat ctgagaagac agcaacagta tcggtatgtt tcatccaatc
    13381 ttggtcagca gccttagtag gcatattttc atcaccagca taggttctcc aaccttcacc
    13441 cagcatgatg aggtttggat tgagggcgcg tgcagccttg taagcttctt cgatagaagc
    13501 cgcatcatgg tctcccatca tatcgaagcg gaagccatcc actttgtagg tatcaactag
    13561 atatttgata gagtcaacta ggagccgttt ggtcatatgg tgggttgtcc ccaaacgtcc
    13621 accaccaaag ctagttcgag gtgtgccatc ggcatccata aagtggtagt agtttggttc
    13681 caaatcttca aagagatcga ctttggctgt gtggttataa acgacatcta ggatagctcc
    13741 cataccacgt ttgtggattt cgttgatgag gtttttaaat tctgcgattc gtttttctgg
    13801 attcttagga tcgcttgagt acataccagt caaggagaag tagttttgag ggtcatatcc
    13861 ccagttgtag ttgctgttgc ttgaagcgta gtcagacaat cgttcatggt ttttcaattc
    13921 attgacaaag tagtaagaca agactggaag gagctggata tgggttacac ccaagtcttt
    13981 gagatagtct agtttttcaa tgaaggcttc aaaagtccca aatggtttgg tcaagtcttt
    14041 tgcaatggca ggatctgaag tgaagtcacg cacatgagct tcgtagataa cggcgtcttc
    14101 acgagtcttg aaattgtgaa tcttaccata agtcaagtct tgaggtccga gtttagctgg
    14161 atctacaaag gcggctttag ccactttatg ggcatcgtca atcttggcat cgtcgctatt
    14221 ccaagcagca agagatttag cgtaaggatc gagtgcaaga acagttttac cttgacgctc
    14281 gatttggtat tgataatagt agccagtgaa atctgtgatt ccgagtttgt ttgtgctgtc
    14341 tagagtttgt ttccaagttc ctctttcccc tttttcgaga gcgacagttc caactacttt
    14401 gtcagggtca ttcttgtcgt agacaacaac agaaacctta tcagcacttg gtgaccaaag
    14461 ggtcaaatca acttgttttc cttcttcttt taggtcagat cccagtttgc catcatagct
    14521 gtatgtctca tctttcaggc gccagcttgt tttggtagtg aattggtcgg aattgtagct
    14581 aacagtataa ggatgttttg tgtcagagaa atctccgctg taggtcactt tcttaccagc
    14641 ttcatcgatt gcaacatcgg taatagttac cttgtttcct aggtgattag tgatgttgga
    14701 gtgtttgagg atatcttctt ttttagcacc gacaagtgtt gaaaagctac tttcaatgct
    14761 agaagtgcct acgtgttggg ctcctgtcat acggatatca tggacatagt atggatttgt
    14821 gtaaatcgat tcatcatcgt cttttaggaa aatttggcta tgatttttca aatctgtgaa
    14881 cttataattt tctttacgga ttttcacgtc gtctccttgt ttgctctcat ctagtaataa
    14941 aaatccaaat tctcttgcgg cttcattaag aggaatgtcg atatagcggc catatttgcc
    15001 tgtagccgta aagtctgttc cgtcaggcca ttgagcgcta cttggatttt tcacatctcc
    15061 ccagtaccag agagatttct tgtcatagtt gccatctgtg cggtagtagt tgacgcgaac
    15121 agttcctgca ggctgtggct cgtaagagaa aaccttgtaa tcttggtcta accaagcctc
    15181 gttcattttt ggaactagtt tttctacaga tttatcgccg gttagatttt ttccagctgt
    15241 attgttgatg aggaagctaa ttttcttggc ttgttctccc tttaatttga catctaggta
    15301 atagccgtag tcatctttct tggcatcctt gaaggacaaa gctccgtttg gccagttttc
    15361 agatggtttt tcaacatcgt cccaagtcca tagtccttga gcatctttgt tttcttcagg
    15421 aagttttttg acatggatac ggaagtagtt atcttcgatg ggctcttctg ccttagtttc
    15481 agttgttact ggactagtag aagtagttgg ctcacttgaa cgatcttgtc cagtttgtgg
    15541 tgctggatcg gctgggcttg cagcaggagt tggtggctct gttgcaggtt tttcttctgt
    15601 tttctctagg gaagcgtttg catttccagg tgaagaaata tcactttcgt tcttaccaga
    15661 gccagctgct gtatcagtaa gaggttgggc aagggtggtt gtatttctgt tgttgggttc
    15721 gttagtggtt ggtgtacttt cgtcggatga gatagttggt gtagccatgg caagcaagac
    15781 aaggcttgcg ccgataagga cagaaccagt cccatttttg agggaacgga tgctgtagac
    15841 catttttttc tcagtgtgag atggtgtttt tctcataatg attctccaat caataaattt
    15901 ctatatcagt atagcatgta gtaaaacaat atgcaagcgt tttctttgat ttgtgaaaaa
    15961 aattcttact tttgagaagt tttctgtact attttatata tcagtataaa tgacggagat
    16021 aaaaatgtca cataagccaa gccgtattat taggaagatg atgtgttccc agctattttt
    16081 attcagaaat tgatctctaa cattaccaat aaaaaagaaa aatatcttga caaacaaggg
    16141 aaaattttgt tacaataata gacggtactt tttacttttg gtctctcaag agtgtacagg
    16201 gacgtgctga caaatgttgc aaaagtacac acagatgata tctgtcacca agtgtatcat
    16261 caccaaaaat aaaaaaacac aggagaatgt agatgcctac aattaaccaa ttggttcgca
    16321 aaccgcgtaa atcaaaagta gaaaaatcta aatcaccagc tttgaacgtt ggttacaata
    16381 gtcataaaaa agttcaaaca aacgtttctt caccacaaaa acgtggtgtt gcaactcgtg
    16441 ttggaacaat gacacctaaa aaacctaact cagcccttcg taaattcgct cgtgtacgtt
    16501 tgagcaacct tatcgaagtt actgcctaca tcccaggtat cggacacaac ttgcaagagc
    16561 acagcgtggt gcttcttcgc ggtggacgtg taaaagacct tccaggggta cgttaccata
    16621 tcgtccgtgg tgcacttgat actgcaggtg ttaacgatcg taaacaaggt cgttctaaat
    16681 acggtactaa acgtccaaaa gcataaggaa aggggataaa gagaaatgag tcgtaaaaat
    16741 agagctccaa aacgtgacgt attgccagat ccgctataca attcacaact agttactcgt
    16801 cttatcaacc gcgttatgct tgatggtaaa cgtggtactg ctgcttcaat cgtttacggt
    16861 gcttttgagc aaatcaaaga agctactggc aacgatgcac ttgaagtatt tgaaacagct
    16921 atggaaaaca tcatgcctgt acttgaagta cgtgcacgtc gtgttggtgg ttctaactac
    16981 caagtcccag ttgaagttcg tccagaacgt cgtacaacac ttggacttcg ttggttggta
    17041 acaatcgctc gtcttcgtgg tgaacacaca atgcaagacc gtcttgcaaa agaaatcttg
    17101 gatgctgcta acaacactgg tgcagcagtt aagaaacgtg aagatactca ccgtatggct
    17161 gaagctaacc gtgcattcgc acacttccgt tggtaagata ggatgcgaaa gcgttaagaa
    17221 agttccagag aaaataggga atcgaagcag gttgcggttg caaccaatga gattcatctt
    17281 tttctccaga cttttagctt gagctcaact aaatcatgat gttaggaacg gtaaggatgc
    17341 aaggtgaaaa taggaaactg acgcagtatt cgacgaatac aaggagtttt atctttttca
    17401 cgcagcatcc cgttccagct cacatcggct aactaacttt agcccgggtt caaattagct
    17461 aaatcgatta gtattagcta taactcagct taccatctcg taagttgaaa ccaacaatag
    17521 catgaaaaca ttgagaacgg gtaggtcctg cctatccgtt tttattaaaa tcgtgttata
    17581 atagaataga aatcaaaaat aaataggaga aacaaacctc atggcacgcg aattttcact
    17641 tgaaaaaact cgtaatatcg gtatcatggc tcacgtcgat gccggtaaaa caacaactac
    17701 tgagcgtatt ctttactaca ctggtaaaat ccacaaaatc ggtgaaactc acgaaggtgc
    17761 gtcacaaatg gactggatgg agcaagagca agaacgtggt atcacgatca catctgctgc
    17821 gacaacagct caatggaaca accaccgcgt aaacatcatc gacacaccag gacacgtgga
    17881 cttcacaatc gaagtacaac gttctcttcg tgtattggat ggtgcggtta ccgttcttga
    17941 ctcacaatca ggtgttgagc ctcaaactga aacagtttgg cgtcaagcaa ctgagtacgg
    18001 agttccacgt atcgtatttg ccaacaaaat ggacaaaatc ggtgctgact tcctttactc
    18061 tgtaagcaca cttcacgatc gtcttcaagc aaatgcacac ccaatccaat tgccaatcgg
    18121 ttctgaagat gacttccgtg gtatcattga cttgatcaag atgaaagctg aaatctatac
    18181 taacgacctt ggtacggata tccttgaaga agacatccca gctgaatacc ttgaccaagc
    18241 tcaagaatac cgtgaaaaat tgattgaagc agttgctgaa actgacgaag aattgatgat
    18301 gaaatacctc gaaggtgaag aaatcactaa cgaagaattg aaagctggta tccgtaaagc
    18361 gactatcaac gttgaattct tcccagtatt gtgtggttca gccttcaaaa acaaaggtgt
    18421 tcaattgatg cttgatgcgg ttatcgacta ccttccaagc ccacttgaca tcccagcaat
    18481 caaaggtatt aacccagata cagacgctga agaaattcgt ccagcatctg acgaagagcc
    18541 atttgcagct cttgccttca agatcatgac tgacccattc gtaggtcgtt tgacattctt
    18601 ccgtgtttac tcaggtgttc ttcaatcagg ttcatacgta ttgaatactt ctaaaggtaa
    18661 acgtgaacgt atcggacgta tccttcaaat gcacgctaac agccgtcaag aaatcgacac
    18721 tgtttactca ggtgatatcg ctgctgccgt tggtttgaaa gatactacaa ctggtgactc
    18781 attgacagat gaaaaagcta aaatcatcct tgagtcaatc aacgttccag aaccagttat
    18841 ccaattgatg gttgagccaa aatctaaagc tgaccaagac aagatgggta tcgcccttca
    18901 aaaattggct gaagaagatc caacattccg cgttgaaaca aacgttgaaa ctggtgaaac
    18961 agttatctca ggtatgggtg aacttcacct tgacgtcctt gttgatcgta tgcgtcgtga
    19021 gttcaaagtt gaagcgaacg taggtgctcc tcaagtatct taccgtgaaa cattccgcgc
    19081 ttctactcaa gcacgtggat tcttcaaacg tcagtctggt ggtaaaggtc aattcggtga
    19141 tgtatggatt gaatttactc caaacgaaga aggtaaagga ttcgaattcg aaaacgcaat
    19201 cgtcggtggt gtggttcctc gtgaatttat cccagcggtt gaaaaaggtt tggtagaatc
    19261 tatggctaac ggtgttcttg caggttaccc aatggttgac gttaaagcta agctttatga
    19321 tggttcatat cacgatgtcg actcatctga aactgccttc aagattgcgg cttcactttc
    19381 ccttaaagaa gctgctaaat cagcacaacc agctatcctt gaaccaatga tgcttgtaac
    19441 aatcactgtt ccagaagaaa accttggtga tgttatgggt cacgtaactg ctcgtcgtgg
    19501 acgtgtagat ggtatggaag cacacggtaa cagccaaatc gttcgtgctt acgttccact
    19561 tgctgaaatg ttcggttacg caacagttct tcgttctgca tctcaaggac gtggtacatt
    19621 catgatggta tttgaccact acgaagatgt acctaagtca gtacaagaag aaattattaa
    19681 gaaaaataaa ggtgaagact aatccgtcct cactctagaa ggaagtcact tagtggcttc
    19741 cttttgtctt tagaaaatac ctctaaatat ggtaaaatag tagaagaata atgtgaggaa
    19801 aatgaatgtc aaatagtttt gaaattttga tgaatcaatt ggggatgcct gctgaaatga
    19861 gacaggctcc tgctttagca caggctaata ttgagcgagt tgtggttcat aaaattagta
    19921 aggtatggga gtttcatttc gtattttcta atattttacc tattgaaatc tttttagaat
    19981 taaagaaagg tttgagcgaa gaattttcta agacaggcaa taaagctgtt tttgaaatta
    20041 aggctcggtc tcaagaattt tcaaatcagc tcttgcagtc ctactatagg gaggctttct
    20101 ctgaaggtcc atgtgctagt caaggtttta agtcccttta tcaaaatttg caagttcgtg
    20161 ctgagggcaa tcagctattt attgaaggat ctgaagcgat tgataaggaa cattttaaga
    20221 agaatcatct tcctaattta gccaaacaac ttgaaaagtt tggttttcca acttttaact
    20281 gtcaagtaga gaagaatgat gtcctgaccc aagagcagga agaggccttt catgctgaaa
    20341 atgagcagat tgttcaagct gccaatgagg aagcgctccg tgctatggaa caactggagc
    20401 agatggcacc tcctccagcg gaagagaaac cagtctttga ttttcaagcg aaaaaagctg
    20461 cagctaaacc caagctggat aaggcggaga ttactcctat gatcgaagtg acgacagagg
    20521 aaaatcgtct ggtctttgaa ggggttgttt ttgatgtgga gcaaaaagtg actagaacag
    20581 gtcgtgtttt aatcaacttt aaaatgacgg actacacttc aagtttttct atgcaaaagt
    20641 gggttaaaaa cgaggaagag gcccagaagt ttgacctcat caagaagaat tcttggctcc
    20701 gagttcgagg gaatgtggag atgaataact tcacacgcga tttgactatg aacgtacagg
    20761 atgtgcagga agttgttcac tatgagcgga aggatttgat gccagaaggt gagcgtcggg
    20821 ttgagtttca tgctcatact aacatgtcga ctatggatgc tttgccagag gtcgaagaga
    20881 ttgttgcaac agctgctaag tggggacaca aggcggttgc tattacggac catgggaatg
    20941 tccagtcctt tccacatggc tataaggcgg ctaagaaagc gggaatccag ctgatctatg
    21001 ggatggaagc caatatcgtg gaggaccgtg tccctatcgt ctataacgaa gtggagatgg
    21061 acttgtcaga agcaacctac gtggtctttg acgtggaaac gacgggactt tcagctatct
    21121 ataatgactt gattcaggtt gcggcctcta agatgtacaa ggggaatgtt attgctgaat
    21181 ttgatgaatt tatcaatcct gggcatccct tgtcagcctt tactacagag ttaactggaa
    21241 ttacagatga tcatgtcaaa aatgccaaac cactagaaca agttttgcaa gaattccaag
    21301 aattttgcaa ggatacggtc ctagttgccc acaatgctac ctttgacgtt ggctttatga
    21361 atgctaatta tgagcgtcat ggtcttccaa agattagtca gccagttatt gatacgctgg
    21421 agtttgctag aaacctctat cctgagtata aacgccatgg tttggggcct ttgaccaagc
    21481 gttttggtgt ggccttggaa catcaccaca tggccaacta cgatgcggaa gcgactggtc
    21541 gtctgctttt catctttatc aaagaggtag cagaaaaaca tggtgtgacc gatttagcta
    21601 gactcaacat tgatctaatc agtccagatt cttacaaaaa agctcggatc aagcatgcga
    21661 ccatctatgt caagaatcag gtaggtctaa aaaatatctt taagctggtt tccttgtcta
    21721 ataccaagta ttttgaagga gtgccacgga ttccgagaac ggttctagat gcccatcgag
    21781 agggcttgat tttaggttca gcctgttcag agggtgaagt ttttgacgtg gtcgtttctc
    21841 aaggtgtgga tgcggcggtt gaggtggcca agtattatga ctttatcgag gtcatgccac
    21901 cggctatcta tgcgcccttg attgccaaag agcaggtcaa ggatatggag gaactccaga
    21961 ccattatcaa gagtttgata gaggttggag atcgccttgg caagcctgtt ctggctacgg
    22021 gaaatgttca ctatatcgaa ccggaagaag agatttatcg tgaaattatc gtccgtagtt
    22081 tgggacaggg tgcgatgatt aaccgaacta tcggtcatgg tgaacatgcc caaccagcgc
    22141 cacttccaaa ggctcatttt cgaacgacta atgagatgtt ggatgaattt gcctttttgg
    22201 gagaggaact ggctcgtaaa ctggttattg aaaacactaa tgccttggca gaaatatttg
    22261 aacccgttga agtcgttaag ggtgacttgt atacgccttt catcgacaag gctgaagaaa
    22321 cagttgctga gttgacctat aagaaagctt ttgagattta tggaaatccg ctgccagata
    22381 ttgttgattt gcggattgaa aaagaattaa catccatact ggggaatgga tttgctgtga
    22441 tttatctggc atcgcagatg ctggtgcaac gttctaatga acggggttat ttggttggtt
    22501 ctcgtgggtc tgtcggatct agtttcgttg cgaccatgat tgggattacg gaggtcaatc
    22561 ctctctctcc tcactatgtc tgtggtcagt gtcagtacag tgagtttatc acagatggtt
    22621 cgtacggttc aggatttgat atgccccata aggactgtcc aaactgtggt cacaaactca
    22681 gtaaaaacgg acaggatatt ccgtttgaga ccttccttgg ttttgatggg gataaggttc
    22741 ctgatattga cttgaacttc tcgggagaag atcagcctag cgcccacttg gatgtgcgtg
    22801 atatctttgg tgaagaatat gccttccgtg cgggaacggt tggtacggta gctgccaaga
    22861 ctgcctatgg atttgtcaag ggttacgagc gagattatgg caagttttat cgtgatgcag
    22921 aagtagaacg cctcgctcaa ggagcggcgg gtgtcaagcg gacaacaggc caacacccgg
    22981 ggggaatcgt tgttattccg aactacatgg atgtctacga ttttacgcct gtccagtatc
    23041 cagcagatga tgtcacggct gaatggcaga ccactcactt taacttccac gatatcgatg
    23101 agaacgtcct caaactcgat gtactgggac atgatgatcc gaccatgatt cgaaaacttc
    23161 aggatttgtc tggtattgac cctaataaaa ttcctatgga tgacgaaggc gtgatggcac
    23221 tcttttctgg gactgatgtg ctaggggtaa cacctgaaca aattggaacg cctacgggta
    23281 tgttggggat tccagagttt ggaacaaatt tcgtacgtgg aatggtagac gaaacccatc
    23341 cgacaacctt tgcggaattg cttcagctgt ctggtctgtc ccacggtact gacgtttggt
    23401 tggggaatgc tcaggatctg attaagcaag gaatagcgga cctatcgact gttatcggtt
    23461 gtcgggacga catcatggtt tacctcatgc atgcgggtct ggaacctaag atggccttta
    23521 ccattatgga acgggtacgt aagggtttgt ggctaaagat ttcagaagag gagagaaatg
    23581 actatatcga agccatgaag gctaataagg tgccagagtg gtatatcgaa tcctgtggga
    23641 aaattaagta catgttccct aaggcccatg cggcagccta cgttatgatg gccttgcgtg
    23701 tagcttactt caaggttcac catcctattt attactactg tgcttacttc tccattcgtg
    23761 ctaaggcttt tgatatcaag accatgggtg cgggcttgga ggtcatcaag cgcagaatgg
    23821 aagaaatctc tgaaaaacgg aagaacaatg aagcctctaa tgtggaaatc gatctctata
    23881 caactcttga gattgtcaat gagatgtggg aacgaggttt caagtttggt aaattagatc
    23941 tctactgtag tcagacgaca gagttcctca tcgacgggga tacccttatc ccaccatttg
    24001 tagcaatgga tggtctgggg gagaacgttg ccaagcaact ggtgcgggcg cgtgaagagg
    24061 gagaattcct ctctaaaaca gaactacgca agcgtggtgg actctcatca accttggttg
    24121 aaaagatgga tgagatgggt attcttggaa atatgccaga ggataaccag ttgagtttgt
    24181 ttgatgagtt gttttaaaaa attgcttaat aatctattaa aagaggctaa cgtatatcca
    24241 atagatttac attagctttc ttttttgtta aaatagtcta tggaaagagg gtgagagtat
    24301 gtcaaagatg agtataagca tccgtctgga tagtgaggtt aaggagcagg cccaacaggt
    24361 gtttagtaat ctgggaatgg atatgacaac agctattaat attttccttc gtcaggcaat
    24421 tcaatatcag ggattacctt ttgatgttag actagacgaa aatcggaagt tgctccaagc
    24481 gttaacggat ttagaccaaa atcgtaatat gagccagtct tttgaatcag tctcagattt
    24541 gatggaggac ttacgtgctt aagattcgtt atcataaaca gtttaaaaaa gattttaagt
    24601 tggctatgaa gcgtggtttg aaggcagaat tattagaaga agttttgaat tttctggttc
    24661 aagaaaaaga acatcctgcc agatatcgtg atcattcatt gacggcatcc aagcattttc
    24721 aaggagtttg tgaatgccat acccagccag attggctttt ggtttataaa gtagacaagt
    24781 cggaattgat tttaaatttg ctgaggacag gcagtcacag tgatttattt taatctattt
    24841 taagggggtt ctcatgaaac taagaatatt tgcggaagat aagccggcta agaaggtatt
    24901 tgaatatcaa ttagaacttg ctgatcgtac aattcttcta tcgacagcac tcttgtcagg
    24961 tgctattgct ttagcaggaa tcttttctgc tttgagagaa aaataaaaat agaaaagaga
    25021 aaacagaatg gttttaccaa attttaaaga aaatctagaa aaatatgcga aattgttggt
    25081 tgcgaacgga attaacgtgc aacctggtca cactttggct ctctctattg atgtggagca
    25141 acgtgaattg gcacatctaa tcgtgaaaga agcttatgcc ttgggtgcgc atgaggtcat
    25201 cgttcagtgg acagatgatg tgattaaccg tgagaaattc ctccatgccc cgatggagcg
    25261 tttggacaat gtgccagaat acaagattgc tgagatgaac tatctcttgg agaacaaggc
    25321 tagccgtctt ggagttcgtt catctgatcc aggtgccttg aacggagtgg acgctgacaa
    25381 gctttcagct tctgctaaag ctatgggact tgccatgaag ccaatgcgaa tcgcaactca
    25441 atctaacaag gttagctgga ctgtagcagc cgctgctgga cttgaatggg ctaagaaagt
    25501 cttcccaaat gctacgagcg atgaagaagc agtcgatctc ctttgggacc aaattttcaa
    25561 aacttgccgt gtctacgaag cagatcctgt taaggcctgg gaagagcatg cagctatctt
    25621 gaagagcaag gccgatatgc ttaataagga gcaattttca gcccttcact acacagcgcc
    25681 aggaacagat ttaacacttg gtttgccaaa gaaccacgtt tgggaatcag ctggtgctgt
    25741 caatgcacag ggcgaaggat tcttgccaaa tatgccgaca gaagaggtct tcacagcgcc
    25801 tgacttccgt cgtgcagatg gttatgtcac ttctacaaaa ccgcttagct acaacggaaa
    25861 tatcatcgaa ggtattaagg taacctttaa ggatggacaa atcgtagata tcactgctga
    25921 gaagggtgat caggttatga aagaccttgt ctttgaaaat gcgggtgcgc gtgccttggg
    25981 tgaatgtgcc ttggtaccag atccaagtcc aatttctcag tcaggcatta ccttctttaa
    26041 cacccttttc gatgaaaatg cgtcaaacca cttggctatc ggtgcagcct atgcgactag
    26101 cgttgttgat ggagcggaga tgagcgaaga ggagcttgaa gctgcagggc ttaaccgttc
    26161 agatgttcac gtagacttta tgattggttc taaccaaatg gatatcgatg gtattcgtga
    26221 ggatggaacg cgagtacttc ttttccgtaa tgggaattgg gcaaattaag gagataatat
    26281 gttaggaagt atgttcgttg gtctcctagt gggattttta gcaggtgcta tgaccaatcg
    26341 tggagagcga atgggatgtt ttggaaaaat gtttctcggt tggatcggag cctttctagg
    26401 tcacttgctc tttggaactt gggggccagt tttatcagga acagctatta tcccagctgt
    26461 tttaggtgcc atgattgtct tagctatttt ttggagacga ggaagttaat ttcctaaatc
    26521 tgatactcaa tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctcaaagcac
    26581 tgctttgagg ttgcagatag aactgacgaa gtcagtaaca tatatacggc aaggcgacgt
    26641 tgacgcggtt tgaagagatt tttgaagagt atgaaacgaa aaggaggttg gtcattgtac
    26701 cagcctcctt ttgatatgat ataatagttc tatgagatta gataaatttt tagttgcctg
    26761 tgcggtggga agtcggactg aggtcaaaaa cttgctcaag gctgggcgcg tgactgtaaa
    26821 tggtaaaaaa gaaaaatcag ctaaattgca gattgatgaa aaaatagatg agattcgctt
    26881 tgatgggcaa gtgttggagt atgaagagtt tgtctactac atgatgaaca agcccaaagg
    26941 agttatctca gcgactgagg atcccaagca cagaaccgtt ctggacttgt tggatgactt
    27001 ggcgcggagt aaggaagttt tcccagtagg acgcttggat attgacacgc atggtctttt
    27061 gctcttgacc aataatggtc agctggctca tgttcttctt tcgcccaagc gtcatgtgga
    27121 caagacttat caggctcagg tcaagggaat tatgacccaa gaagatgtgg agacatttat
    27181 caagggcatt ccgctcaaag actttacctg tcagcccgct agactggaga ttctttccac
    27241 agatgcagaa aagaatcaga gccaaatccg tgtgaccatt gcagaaggga agtttcatca
    27301 gatcaagcgt atggtgggct actgtggcaa ggaagtggtg gacttgcaac gattgactat
    27361 gggaacttta gtattagatg agaacctgga acgaggggaa tggcgtcgct tgaccaagga
    27421 agaattagaa attctccgcg ctaatattat ttaatttagt ttaatactca atgaaaatca
    27481 aagagcaaac taggaaacta gccgcaggct gtacttgagt acggcaaggc gacgttgacg
    27541 tggtttgaat ttgattttcg aagagtataa attcgaaaaa ggtgagggac aatgtcctcg
    27601 ccttttatgt tttttagttg cttcctttgt gaaaagagtt ataatagact gtagaataaa
    27661 aggaggaatc tatgaacgcg attcaagaat catttactga taaactattt gccaactatg
    27721 aagcaaatgt aaaataccaa gcgattgaaa atgctgccag ccacaacgga atttttgcag
    27781 ctctggaatg tcgccaaagc catgtagaca acacacctgt tttctcattg gatttaacca
    27841 aggacaaggt cactaaccag aaagcgtctg gtcgttgctg gatgtttgcg gctctcaaca
    27901 ccttccgcca caaactcatc tcgcaataca aattggagaa ctttgagttg tcacaagccc
    27961 acactttctt ctgggataag tatgagaaat caaactggtt cttggagcaa gtcattgcga
    28021 cttcagacca agaattgact agccgcaagg ttagcttctt actccaaaca cctcaacaag
    28081 atggcggtca atgggatatg gtcgtttccc tctttgaaaa atacggtgtc gtgcctaagt
    28141 cagtttatcc tgagtctgtt tcatctagca gcagtcgtga gctaaatgcg atccttaata
    28201 aattgcttcg tcaagatgct caaatcttgc gtgatttgct tgtttctggt gcagatcaag
    28261 cgactgttca agctaagaaa gaagacctct tgcaagaaat ctttaacttt cttgctatgt
    28321 cattaggact tccaccacgc aagtttgact ttgcttatcg cgataaagat aacaactata
    28381 aaagtgaaaa aggaatcaca ccacaagagt tttacaagaa atatgtcaat cttcctttag
    28441 aagactacgt ttctgttatc aatgctccaa ctgctgataa accttacgga aaatcttaca
    28501 cagttgagat gttggggaat gtggttggta gccgtgcagt tcgctacatc aacgttccaa
    28561 tggagcgctt gaaagaattg gcgattgccc aaatgcaagc aggtgagact gtttggtttg
    28621 gttctgatgt cggccagctc agcaaccgta aggctggcat ccttgcgaca gatgtttatg
    28681 actttgaatc aagcatggac attaaactta ctcaagacaa ggctggacgt ttggactata
    28741 gcgaaagctt gatgacccac gccatggtct tgacaggtgt tgacttggac gaaaatggta
    28801 aatcaaccaa gtggaaggtt gaaaactcat ggggagacaa ggtcggtaca gatggttact
    28861 ttgttgcctc agacgcttgg atggacgaat acacatacca aatcgttgtt cgtaaggaat
    28921 tgctgacagc agaagaacaa gctgcctatg gagcagaaac aatcgtactt gcaccatggg
    28981 atccaatggg agccttggct gaataaaagc atagaaaaaa ggaatcagat tttagaacct
    29041 ggttcctttt tagttgcttg attacatgat gtgaagaaca tgtgccacaa tacccactgc
    29101 gaagagggca aggataatag tgattggaga tacttttttc ttaagtaagt acatgcaaag
    29161 taaagtaagg agtagtcctg ataatccagg aatcaacata tccaagtttt gttggaaagt
    29221 agtaactttt tcaggagttt gagacaatcc ttgtcctact tgtgcgaatg cttcttggat
    29281 acctttagac ccttctggca atttatccca atggatataa gccttttcat ctagttgaac
    29341 tttagaaaca tcgaaagcaa atttaatatt tacccagcgt tgaacaagga cagcaagaat
    29401 gaacatccca aggatagaag ctcctttagt gatatcttga aggataccac cagacatatc
    29461 tttagtgatt tctgatccag ccttgtatcc aatctcttga acataccaca agaatgacat
    29521 acgaatcaag ttccatgcaa caaagaagag gagtggaccc aagatattgc cagtaagggc
    29581 aagtgaagca ccgagagatc caaggattgg gcgcactgta aaccagaata ctgggtcacc
    29641 gataccagca agaggtccca tcataccgat tttaacccct tggatagcag cgtcatcgat
    29701 ttccacaccg ttagcacgtt cttcttcaag cgcaagagta acccccatga ctggagcagc
    29761 tacgtatgga tgagtgttga agaactcaag gtgacgctca agagcagcga tttgatcttc
    29821 ttttttagta tagagttttt tgatagctgg aatgagtgta taagcccagc ccaagttttg
    29881 catccgttca aagttccaag acccttgtaa gaaggttgaa cgccaccaaa cttttttacg
    29941 atctgattta gttaattgaa gtttttcagt catgatgttt tcagtccttt cttatcttag
    30001 tagtcttcta ggatatcgcc gattgggtcg ttagaagttg cggctcctcc gccaccattt
    30061 ccaccagttt tagaaaggtg aaggtagata agagcgatag caacaccgat agcaccgaat
    30121 ccgattagag taatatctga cacagcagcg agaacgaaac caagagcgaa gaatggccat
    30181 acttcacgag ttgccatcat gttgataacc atggcgtaac caacggcaac gaccatacca
    30241 ccaccgatag ccataccatc tttgagccag tctggcatgg cactaaggat actttgtaca
    30301 gtttcagttg gtaccataag gagaagagct gcaggaagcg cgatacgaag tccttggaaa
    30361 agtagcgcaa tgaaatgcgc acgctccaca gcgccgaagt cacctttttt agcggcagca
    30421 tctgcagtat gaaccaaacc aactgaaatt gtacgaacaa tcattgtcaa gaaaagtcca
    30481 gctacggcaa gagggatagc aaccgcttgg gcaacaccga taccagtctt ggtaaagtca
    30541 ccaccaagaa ccatgataat ggcagcagcg acagaagcaa gtgcagcatc aggagcgata
    30601 gcagcaccga tatttgacca accaagggca atcatttgaa gcgatccacc gaggataatc
    30661 cctgcttcca agtgacctgt tacaagccca ataagggtac aggctacaag tggttggtga
    30721 aattggaact ggtcgaggat gccttcaaga cctgcaaaga aggctacaac gactactaaa
    30781 accatagaaa taatagacat gtttaaaatc ctttcataaa taatggctta tttgacattg
    30841 gctttgttaa tcaagtcaaa caaatctttt ttagaatcat ttggtacttt acggacatca
    30901 aattcaacac ccaagtcacg cattttttca aatgtagcaa catcttcttt gtccatagac
    30961 aaaacggtat tgaccaatgt tttacctgtt gagtgagcca tagaaccaac gttaagagtc
    31021 ttgattggca cgccgccttc gatggcacga agggcatctt gaggtgtttc aaacaagata
    31081 agggcatgtg tttctccaaa acgtgggtct tttgaaatat caatcagttt ttgaattgga
    31141 accacgttag ccttgacatt acctggagct gcttgtttaa tcaattcttt acgaaggtcg
    31201 tctttagcca cgttatctga agcaacgatg atacgatttg cttttgaatc tggagtccaa
    31261 gcagttgcaa cctgaccgtg aagtagacgt gtgtcaagac gggcaagatt gattttcaat
    31321 ttaccgtctc cgataacagt tccttctggg atagcagctt gggcaactgg agcagctgca
    31381 gcgcttgcaa cttcttcaac tggatttagc tcttctggaa gagctttgat gccatctttg
    31441 gcttctttaa tgatattagc aacgactttt tctacacctg cagcagcgtc catgaggcgc
    31501 tctgtgtagg cttgaatcaa catcggtaag ttaagtcctg tgatgatggc aaacttacgc
    31561 tcaggatttt ctcccatcac gcgactagct tggttaaatg gagaaccact ccaaaggtca
    31621 gccaaaacta gaacctcatc ttctgcgtca aatgcagcaa cagcgttatt aaacttagcg
    31681 tatagatcat caggaccttc atttggcata aaggtcacaa cttgaacctt ttcttgttca
    31741 ccaaagatca tagatcctga ctgatgaata cccgcagcaa attcgccgtg gctcgcaata
    31801 atgattccga tactcattat tgtcattcct ccttttttgt tttagttttc atttaagaaa
    31861 actttaagac tgtttaagta taaaccgttt tcataaaaaa agcaactatt tatcataaaa
    31921 aattaaaaaa tttttatttt tgtatgttga tatgttgagt ttttgaggag taatcttgaa
    31981 ttcttattta aaatttaata taaaaaagtt ggaagaagct tccaactttt aaaataggtt
    32041 tctattagat tagtgggtga agtcgagtac catacgtcct tggatttggc ctttttccat
    32101 ttcgtcgaaa atggcaacag catcttctac tggacgtttt tgaacaactg ggactaccag
    32161 accttctgca ccaaattgga aggcttcttc taagtcttta cgagttccga caagagaacc
    32221 gatgacttgg attccatcga ggactgtttt aacgatgctg agttccatca tttcagaagg
    32281 aagaccaaca gcgacgacgc gaccaccagc acgaatggag tcaacagcct ggttgaaggc
    32341 aactttagac acagcagtta cgacagctga atgagctcct ccatcagttt tttctttaat
    32401 gagtctagct acatcttcaa cttcgaggcc gttaatcaca atgtcagcgc ctacttcttt
    32461 tgcaagggca agtttgtcat tgttgatatc gactgcgata acatgagcat tgaatacttt
    32521 tttagcgtat tgaacagcga ggttaccaag tccaccagca ccgtaaagaa caacccattg
    32581 gcctggttca acttttgctt ctttgatagc tttataggtt gttactccag cacatgtgat
    32641 agaagaagct tgggctggat caagtccgtc aggaaccttg acagcatagt cagcagttac
    32701 gatacattgt tcagccatac caccgtctac tgagtagcca gcatttttca ctgtacggca
    32761 aagggtttcg cgaccagttg tacagtattc gcaagtgcca catccttcaa agaaccaagc
    32821 aacgctgacg cggtcaccga ctttaaggct tttcacatct ggagcaatct ctttaacgat
    32881 accgatacct tcgtgcccaa gaacacgtcc tgggacttga ccaaagtcac catgagcaac
    32941 gtggaggtcg gtgtggcaaa cgccacagta ttcaacttct acaagtgctt ccccagtttc
    33001 aagtggacgg agtacttttt cttcaatagc aacaccagtg ctttctggat ttacaacaac
    33061 agctttcata gctatcctcc ttgatattga ccgagaacag ggacaacggg actggattga
    33121 ttttttgtca acagagtcga gtgtgacgag ctctcttgta tttatatgtg aagtatatca
    33181 caaaagaaag ccttttccaa ggttttggag acaaaaagta gaacaatcga ttaactggtt
    33241 gatgagactg tgaaaagagc acaaagacca gcttgcaagc tggtcatact cttcgaaaat
    33301 ctcttcaaac cacgtcagct tcatctacaa cctcaaagca gtgctttgag caacctacgg
    33361 ctagcttcct agtttgctct ttgattctca ttgagtatca gtataggtta taagaataag
    33421 tcttgtaggg ttttggcaac cccatcttgg tcgttggtaa gggaaatttg ctcatctgca
    33481 taagggagta gctctggatt ggcatttttc atggcataac ccttcccagc aaaagcgagc
    33541 atttcggtat cattgtgttc atctccaaag gcaatcaaat cttttttgtc acgattcatt
    33601 atcttgagca agtagtccaa agcaaaggcc ttgttgacac cttttggggt acattcaagg
    33661 atattgagcg gacctcccca ggtattgata gaaagttgat gctggtagaa ggcgttcatg
    33721 tcttttgcca aggcatattt gtcactggct ctggtctgca agaggataca gttagggttc
    33781 ttggtcacca attcaggctg gaattgatct tcaggctgga aagcttctac accaaatagt
    33841 ttgggattgg caatttcttc attgggattt gtaatgtaga attttttacg atattctcca
    33901 gcgataaaat cggcttgaat gtcctctgaa cgttgaacca tatctagcag atattttttg
    33961 tctacagtca aacacttttc aaaatcccaa acttggtctg gtaaatgagt aagggatccg
    34021 ttgaagttaa tcataggagt gtctaagccc agttcacggt aaaaatcttt tgacatacgg
    34081 taagggcgac ctgtcgtaat aataacctga tggccttttt cagcaacttt tttgatggtt
    34141 tttttggtaa agtcagaaat ttgactgtct gagttgagca gggttccatc caggtcaact
    34201 gcaataattt ttttcgtcat atggaccttt ctagtgtgtt ttcagataag atgtcttcta
    34261 ttataacaga aagcaggcag aaaagcagat tccaaacaaa aaatgaaatt ggattaattt
    34321 cttaaacaaa tcaaacaaag acattgaaaa agtgaatgat aaatgatata attctattat
    34381 tgttcgtaaa aattaaaagg agattgatga tggacaaatt atttaaacta aaagagaacg
    34441 gtacagacgt tcgtacagag gttctagctg gtttaacaac tttctttgca atgagctata
    34501 ttctctttgt aaacccacaa atactttcac aaacaggaat gcctgctcag ggcgtcttcc
    34561 tagcgacgat tattggtgca gtagcgggta ccttgatgat ggctttttat gctaacttac
    34621 cttatgccca agcgccaggt atgggactca atgccttctt tacctttaca gttgtattcg
    34681 ggcttggtta ttcttggcaa gaagccctag ctatggtctt catctgtggg attatttcat
    34741 tgattattac cttgacaaat gttcgtaaaa tgatcattga atcgattccc aatgctcttc
    34801 gctcagctat ttcagctggt atcggtgtct tccttgccta tgtagggatt aagaatgctg
    34861 gacttttgaa attcacgatt gatccaggca actatactgt tgtaggagaa ggggctgaca
    34921 aagctcaagc aacgattgca gcaaactctt cagcagttcc aggattggtc agctttaata
    34981 atccagctgt tttagtggct cttgcaggac ttgccattac tatcttcttt gtcatcaaag
    35041 ggattaaagg gggaattatt ctctctatct tgacaacaac tgttcttgct attgcagttg
    35101 gtttggttga tttgtctagt atcgattttg ctaataacca tgttggtgca gcttttgaag
    35161 atttgaagac aatctttggt gcagctcttg gttcagaagg tttgggagct ttggtttcag
    35221 atacagctcg cttgcctgaa actctgatgg ccattcttgc cttctcattg acagatattt
    35281 ttgacacaat tggtaccttg atcggtacag gtgaaaaagt tggtatcgta gcgacaaatg
    35341 gtgaaaatca ccaatcagcc aaattggata aggctcttta ctctgatttg attggaacga
    35401 cagtcggtgc cattgcaggt acttcaaacg taacgactta tgttgagtct gctgctggta
    35461 tcggtgcagg tggacgtact ggtttgacag ccttggttgt agctatctgt tttgcgattt
    35521 caagcttctt tagcccactt ctagcgatcg taccaacagc ggctacagct ccaatcttga
    35581 ttatcgttgg gattatgatg cttggtagct tgaaaaatat ccattgggat gatatgtctg
    35641 aagcagttcc tgccttcttc acatctatct ttatgggatt cagctactct atcactcaag
    35701 ggattgcagt tggtttcttg acttacactt tgactaagct tgttaaaggt caagttaaag
    35761 atgttcatgt catgatttgg attttggatg ccttgtttat ccttaactac atcagcatgg
    35821 ccttataata gaatgaccca gggggatttc cccccttttt taatacaaaa ggagataggt
    35881 gatgaaagag aaaaatatgt ggaaagaatt gttgaatcgt gcaggctgga ttttggtctt
    35941 tttacttgcc gtccttttat atcaggttcc cctagtggtt acctctattt tgactttaaa
    36001 agaagtagcc ctgctacagt cagggctgat agttgctggc ctttcaattg tggttctggc
    36061 tctatttatt atgggagctc gtaaaaccaa gttagctagt tttaattttt ctttttttag
    36121 agctaaagat ttggcacgtt tgggcttgag ttatttagtt attgtcgggt caaatatact
    36181 tggttccatt ttattgcaaa tgtcaaatga gacgacttta ttgcaaatgt caaatgagac
    36241 gacaacagct aaccagtctc agattaatga tatggttcaa aatagttcgt tgatttccag
    36301 tttcttcttg ctagccttgc ttgctccgat ttgtgaggaa atcttgtgtc gtgggattgt
    36361 tcctaaaaag atttttcgag gcaaagagag ctggggattt gtagtcggta cgattgtgtt
    36421 tgctttatta catcaaccaa gtaatttacc ttctttattg atttatggag gtatgtcgac
    36481 agttctatct tggacagcct acaagaccca acgtttggaa atgtcgatct tacttcacat
    36541 gattgttaat gggattgctt tctgtttgtt ggctcttgtg gtgattatga gtcggacatt
    36601 agggatttct gtttaagatt attgacaatt gcttacttgt ttctactggg agaaagatga
    36661 atgaaatcgt gtccatcttt ttctttttat ggtaaaatag agaaataata tgatgaaaag
    36721 ccttgaggga gtgaccgata tgtcaagtaa agccaatcat gcaaagacag ctatttgcgg
    36781 aattatcaat gtaaccccag actccttttc ggacggtggt caattttttg ctcttgagca
    36841 ggcgctccag caggctcgta aattgataac agaaggagcc agtatgctcg atatcggcgg
    36901 agaatcgact cggccgggaa gtagctatgt tgagatagaa gaggaaatcc agcgtgttgt
    36961 tccagtgatc aaagcgattc gcaaggaaag tgatgtactc atttctatcg atacttggaa
    37021 aagtcaggtg gcagaggctg ctttggctgc tggtgccaat ctagttaatg atatcactgg
    37081 tctcatgggc gatgaaaaaa tggctcatgt ggttgctaaa gctggagcga aagtagtcat
    37141 catgtttaat ccagtcatgg ctcgacctca acaccctagc tcgctcatat tccctcattt
    37201 tggatttaat caaactttta cagtagaaga gttagctgac tttgaaacat tgccaatcga
    37261 agacttgatg gtggctttct ttgaacgagc actagcgaga gcggcagaag ctggtattgc
    37321 accagaaaat atcctgttgg atccaggaat tggctttggt ctgaccaaga aagaaaatct
    37381 cctcctttta cgggacctgg ataaactaca tcagaagggc tatccaatct ttctcggagt
    37441 gtcgcgcaag cgatttgtca tcaatatcct agaggagaat ggttttgaag tcaatcctga
    37501 gacagagctt ggtttccgaa atcgggacac ggcttcggct catgtaacta gtatcgctgc
    37561 gagacagggt gtagaagtgg tgcgcgtgca tgacgtagct agtcacagga tggcagttga
    37621 aattgcctct gccattcgtc tggctgatga agcggaaaat ttagatttaa aacaatataa
    37681 ataagatgaa agaaattgaa aacaatcagt ggattgctaa ctaccggacg gatcaaccgc
    37741 attttggctt ggaacgaatg gtggaactgt tagctttgcg tggcaatccc catctcaaac
    37801 tcaaggtcct ccatatcgga gggactaacg gcaagggatc gactattgct tttttgaaaa
    37861 agatgctaga aaagctaggg ctgagagttg gcgtgtttag ctcgccctat ctcattcatt
    37921 acacagacca gattagcatc aatggggaat cgatctcaga agcgaggcta gaagctctca
    37981 tggcagacta tcagtctttg ctggagggag aagcggtcgc caatttacag ggtacaaccg
    38041 agtttgagat tatcacagcc ctggcctatg actactttgc ctcagagcaa gtagatgtgg
    38101 ccatcatgga agttggcatg ggtggacttt ttgatagtac caatgtctgt cagcccattt
    38161 tgacaggaat tacaactatt ggcttggatc atgtggcctt acttggtgac accttggagg
    38221 ccatagcaga gcagaaggca ggtattatca aacaagggat gcccttggta acagggcgta
    38281 ttgctccaga agccttggct gtgattgacc gcattgcgga agggaaagat gcgccgagac
    38341 ttgcctacgg gacagattat caggttcgtc atcaagacag tgtggtgaca ggggaagtct
    38401 ttgactatac aagtgctgtc agacaaggtc gcttccagac tagcctgctt ggtttgtacc
    38461 aaatagagaa tgctgggatg gccatagctt tacttgatac tttttgtcaa gaagatggtc
    38521 gagagctagc aagcaatgac tttcttggtc aagccttgga agaaacaagt tggccagggc
    38581 gtttggaaat cgtgtcaaga gatcccttga tgattttgga tggagcccat aatccccatg
    38641 ctatcaaggc cttgttggta accttgcaag aacgttttgc ggattatcat aaggaaatcc
    38701 tcttcacttg tatcaaaacc aaagccttgg aggatatgtt ggacttgctg ggagccatgc
    38761 cagataccga gcttactcta acacattttg cggatagtcg ggcgacggat gaaagcgtgc
    38821 tgaaagaggc agctaagtct agaaatctca gctaccaaga ttggcatgat tttctagagc
    38881 agaatgtgac agataaaaaa gaagagaaac aaacagttag gattgtcaca ggttccttgt
    38941 atttcttgag ccaagtgagg gtctatctga tggagaggaa gaacgagaat ggatacacaa
    39001 aagattgaag cagctgtaaa aatgattatc gaggctgtag gagaggacgc taatcgcgag
    39061 ggtttgcagg aaacacctgc tcgtgtagcc cgtatgtatc aagagatttt ttcaggtctt
    39121 ggtcaaacag cagaggaaca tttgtcaaaa tcctttgaaa ttattgacga taatatggtg
    39181 gtagaaaagg atatcttttt ccataccatg tgtgaacacc acttcttgcc attttatggt
    39241 agagcgcaca ttgcctacat tccagatggt cgtgtggcag gcttgtctaa gctagcccgt
    39301 acggttgaag tttattcgaa aaaaccacaa attcaagaac gtttgaatat cgaagtggcc
    39361 gatgccttga tggactatct aggtgctaaa ggagcctttg ttatcattga ggcggaacat
    39421 atgtgtatga gtatgcgtgg tgttagaaaa ccaggcactg caaccttgac gacagtagct
    39481 cgtggtctat ttgaaacaga taaggacctc cgcgaccaag cttatcgttt aatggggcta
    39541 taaaaagaat ccgtttcaag cggatttttc tagaaaggaa tcattatgga tcaactgcag
    39601 attaaggatt tggaaatgtt tgcctatcat ggtctttttc ctagtgagaa agaattgggg
    39661 cagaaatttg tcgtttcagc catcctatcc tatgatatga ccaaggcagc tacagacttg
    39721 gatttaacag cctctgtcca ttacggagaa ttgtgtcagc agtggacgac ttggtttcag
    39781 gaaacgagtg aagatttgat tgaaacggta gcctataaac tggtggaacg tacctttgag
    39841 ttttatcctc ttgtccaaga aatgaagttg gaactgaaaa aaccttgggc accggtgcat
    39901 ttgtcactag atacttgctc ggtaaccatt catcgccgca agcaacgagc ctttatcgcc
    39961 ttaggaagca atatgggaga taaacaagca aacttgaagc aagccattga caaactgcga
    40021 gctcgtggca tccatattct caaagagtcc agtgtcttag cgacggagcc ttggggtgga
    40081 gtggagcagg atagctttgc caatcaagtg gttgaggtgg aaacctggct accagcacaa
    40141 gacttgttag aaaccttgtt agccattgag tcagagctgg gacgggtgag agaagtgcat
    40201 tggggacctc gtttgattga tttggacttg ctctttgtgg aggaccagat cctttataca
    40261 gacgacctca tattgcctca tccttacata gcggaacgcc tttttgtcct tgagtcctta
    40321 caggaaattg cgcctcattt tatccatccg atattaaaac aaccgatccg caacttgtat
    40381 gatgctttga aaaaatagaa aaactctagt tttcagtcac ttgcaactga aggctagagt
    40441 ttttaaaata ggtcattttc ttctgggagg aggatagttt ctctaccgtc catgtctaaa
    40501 accagtactc ttgggggata acgagggtcg aaaggatggt taaagtcaaa atcaatggct
    40561 gtagggaggt gttgacttga aaagtggaag gtaatctttc cttggttatt aagcaattga
    40621 aactcgagtt cttcttccaa ttcaaagaca ttttttaaga aatggtcgat gatataccaa
    40681 aaagagtcaa tgatgtcatc aggcaagctg gtaacaatgc caaaactagc agaccgcatg
    40741 tgggtattgg taaaagccat atatctgtcc cctttctttt cccttatcat acagcaaata
    40801 ggattaaaaa tcaagaaaag gtgatttttt gaaaaggatt ttagttacag ggagaaatag
    40861 ggaaaaattc ccaaaaatct accgaagtta ataggtaact tcccaaatta acttgattat
    40921 ataactttca gttattttga gaagttaccg aaaaatattt ttcatatcta ttgactttta
    40981 ggggtgaaat ttggtatgat agtaggcggt attgtttacc ccatttgaaa ggccccggaa
    41041 ccttccaaat acttttcgat gggaaggaac acccatcacc gtaaacaaaa atcgaactat
    41101 atataggaga aatcatgaac aaaacaacat ttatggctaa accaggccaa gttgaacgta
    41161 aatggtacgt agttgacgca actgatgtac cacttggacg tctttctgca gtagttgcta
    41221 gcgtacttcg cggaaaaaac aaaccaacat ttacaccaca cactgataca ggtgacttcg
    41281 tgattgttat caatgctgaa aaagttaaat tgactggtaa aaaagcaact gataaaatct
    41341 actacactca ctcaaaccac ccaggtggat tgaaacaaat ctctgcaggt gaacttcgtt
    41401 ctaaaaatgc agtacgtttg attgagaaat cagttaaagg tatgcttcca cacaatacac
    41461 ttggacgcgc tcaaggtatg aagttgaaag tatttgttgg agctgagcac actcacgctg
    41521 cacaacaacc agaagttctt gacatttcag gacttatcta aggaaaggaa caataaagta
    41581 tgtcacaagc acaatatgca ggtactggac gtcgtaaaaa cgctgttgca cgcgttcgcc
    41641 ttgttccagg aactggtaaa atcactgtta acaaaaaaga tgttgaagag tacatcccac
    41701 acgctgacct tcgtcttgtc atcaaccaac cattcgcagt tacttcaact gtaggttcat
    41761 acgacgtttt cgttaacgtt gtaggtggtg gatacgctgg tcaatcagga gctatccgtc
    41821 atggtatcgc tcgtgccctt cttcaagtag acccagactt ccgcgattca ttgaaacgcg
    41881 caggacttct tacacgtgac tcacgtaaag ttgaacgtaa gaaaccaggt cttaagaaag
    41941 ctcgtaaagc atcacaattt agtaaacgtt aattcgaaag aattactata cttatacaga
    42001 gcacctttcg gggtgttctt tttttatact ttcttactaa attggtgcaa ttgacacagt
    42061 tgttgcgact ttagtcgctt acaaatgtgg ctgcaacctg acaaggtcag ttgcctcaaa
    42121 acgttaatca atacgattat atcaacgttt caaagcactc aaaagtttac cttatgggtg
    42181 cttttgtctt gttcttgttt caattgacta taaatcgcta taagaaaaaa tcattttatt
    42241 ggtataaaga agtgattgaa agtaatggcg aaacgttaga taattagttc ataaaaaaca
    42301 agtttagatc ctcatctaaa cttgtttttt aaaaattgaa ggatgatttt ggaggatata
    42361 agatgacatt gactattcat gtatattata aaggtgatgg agatagtgca ataaagtttg
    42421 caagagaaat gcttgatagt ggccttgttg aagaaattag aaatcaaaaa ggtaacttga
    42481 aatacgaata ttttttacca attgaaaaag agggaactat tctccttatt gatcagtgga
    42541 taaatcaaga agctttagat aagcatcatc aatcaaagac aatgcagaaa atcttagatt
    42601 taagaaaaaa atatcacttg cagatgcaag ttgaacgtta tatagaagat gattctggga
    42661 tgccagaaag cgataagtcg tttatagaca cagggaatta gaattattgt gagagttttt
    42721 gtagtttgaa agtattgaaa atcgtaaaga tatcattacg agaatacaag aagatattgt
    42781 tctatttcca ttaaaaatta cttctttcct taacaataag gaaaaagaat gcgttttcat
    42841 cttttggttt gaggtttata ataagagtat caaagtcagg aggaaaaaag atgactactt
    42901 ttaaagatag atttttatgg ggtggtgctg ttgctgctca tcaacttgaa ggtggatggc
    42961 aagaaggtgg caagggaatt agtgttgctg atgttatgac tgctggtcgt catggagtag
    43021 ctcgtgaaat tactttggga gttttagaag gtaaatatta tccaaatcat gaggcgatag
    43081 atttttatca tcgttataaa gaagatatag cactttttgc tgaaatggga ttcaagtgct
    43141 tccgtacctc tcttgcatgg acacgtatct ttccaaaagg tgatgagtta gagccgaatg
    43201 aagaaggatt acagttttat gataatcttt ttgatgaatg cttaaagaat ggtattgaac
    43261 ctgtcatcac tctatctcat tttgaaatgc cttatcactt agtgaccgaa tatggtggtt
    43321 ggaaaaatag gaaattgatt gatttctttg ctcgttttgc agaagtcgta tttaaacgtt
    43381 acaaagataa ggttaaatat tggatgactt tcaatgaaat caataatcaa gcgaattatc
    43441 aggaagattt tgcaccattt actaactcag gtattgtata tgaggaaggt gataatagag
    43501 aggcaattat gtatcaagca gtacattacg aattagttgc ttctgcacga gctgtaaaaa
    43561 ttggtcatga gattaatcca gattttcaaa taggttgtat gattgcgatg tgtccaattt
    43621 atccagctac ttgcaatcct aaggatatct taatggcaat gaaagctatg cagaagcgtt
    43681 attattttgc tgatgtgcat gttttaggta aatatcctga gcatattttc aagtattggg
    43741 aacgaaaagg tatttcagtt gattttactg cccaggataa agaagattta cttggtggga
    43801 ctgtagatta cattggtttc agttactata tgtcctttgc tatcgactct catcgtgaaa
    43861 ataatcctta ttttgattat cttgaaacag aagatttagt gaaaaataat tatgttaagg
    43921 cttctgaatg ggagtggcaa attgatccag aaggtttacg ttatgcgtta aattggttta
    43981 cagaccacta tcacttacca ctctttattg ttgaaaatgg ttttggagct atagatcaag
    44041 ttgcagcaga tggtatggta catgatgatt atagaattga atatctaggt gcccatattc
    44101 gtgaaatgaa aaaggctgta gttgaagatg gtgttgattt aatgggttat actccatggg
    44161 gatgtattga tttggtttca gctggtaccg gtgaaatgcg gaaacgttat ggctttattt
    44221 atgtagataa agatgacaat gggaagggaa gttataatcg ttccccgaaa aaatcttttg
    44281 gctggtataa ggaagttatt tcatctaacg gtgaatcagt agaatagtat aaacaaactg
    44341 agattattct cagtttgttt agcatattga ggtgacatat gattggaagt attgcagcaa
    44401 ttttaacaac atttgcattt ttaccacaag tttttcgtgt agttaagaca aaggacacag
    44461 gaactattgc tctaggtatg tatgttatgc aagttattgg tattgcattg tggcttgctc
    44521 atggtatttg tataggagac ttgcctttga ttttagcaaa tagtgtttca tttctactct
    44581 caggaataat tttattttat aaattgaagt ataaataatt tttttccata tcatttagga
    44641 aaatgaggga attgtcattg gaaacgcttt attttataat aaaaatagat aacaaataag
    44701 ccaacttagc acagctttga ttggatttaa gaaaaggaga aaatattatg gcaaaagctt
    44761 taattatttg tgcagcagga atgtcttcat cacttatggc acagaaaaca acagagtttc
    44821 taaaaggaaa agggaaagat attgtagttg atgctattag tgctactgaa ggagggaaag
    44881 ctatcgcagc agcagctttt gatttgtatc ttgtcagccc tcaaacaaaa atgtacttta
    44941 agcaatttga agaggctggt tctaaagcag gaaaaccagt tgtacaaata cctcctcaag
    45001 catatattcc tattccaatg ggaattgaaa aaatggctaa tttgattctt gagaatattt
    45061 gatgaacaca tgaaatgtca ataaatattt aggttaagat aaattgtttt gagtttgata
    45121 taaactgatt taagtgtata atatgcttca atatatgaaa acatacgttt taggagggaa
    45181 tgaatgtgtt aaacattaaa gagaaagata tccttcaatt tttaataaaa aataaggaac
    45241 gttttgttac aagcaaagaa ctggcagaat atttatcctg ttcagataga actgtccgta
    45301 atgtactgaa attaattgag aagacaatga ttattcaagg agtccggttg atttcaaaac
    45361 aaggtcaagg gtatcaaata ttttttgaga atcaaggcgc ttaccaggag tttagacaaa
    45421 cttatgaact ggaagaagac tatacgaaaa ctgcggtttc aaaaggtgat gatcgtttag
    45481 tatttatctt aaataaatta ttatttgagc aagttccagt tctttttgat gatttagctg
    45541 atgaacttta cgttagccgt tcaacattat ctcatgattt cagaaagata cgagtgatgt
    45601 tatcagagta taatttgtcc attgagagta gggcgaataa aggagtttat gtctcaggcg
    45661 aggaacgtga taaacgacgt tttatcataa attatttcct agagaatcaa ttttttaaaa
    45721 caatacattg ctatgtaaaa tttaactttt ttgatcaaac tgttccatta gaagaatttg
    45781 ctagaattgt tttggatgaa tgccaagagg ccaacctaaa actatctgat tttgtactac
    45841 agaatttagt agttcacata gcattgtctg tgattcgttt aaaattcgga tttgaaataa
    45901 aaaatataga ctgtcagatg actgatgatg caatagagag aaaggttgcc caaagaattc
    45961 tttctaaagt aagagaagta acaaatcagg aatttccagt tcaggaaata gactacatta
    46021 ctttgcattt gttagctaag agtcaacaat gtcaaaaaaa tcagaagaat
//
LOCUS       DAVKAO010000008        45531 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKAO010000008 DAVKAO010000000
VERSION     DAVKAO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.008"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(366..1094)
                     /locus_tag="VWX16_000471"
     CDS             complement(366..1094)
                     /locus_tag="VWX16_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9157882.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1252..2661
                     /locus_tag="VWX16_000472"
     CDS             1252..2661
                     /locus_tag="VWX16_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9157883.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTALTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2679..3974
                     /locus_tag="VWX16_000473"
     CDS             2679..3974
                     /locus_tag="VWX16_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9157884.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3979..4287
                     /locus_tag="VWX16_000474"
     CDS             3979..4287
                     /locus_tag="VWX16_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9157885.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALEWIGEIR
                     "
     gene            4284..4592
                     /locus_tag="VWX16_000475"
     CDS             4284..4592
                     /locus_tag="VWX16_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9157886.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(5459..8110)
                     /gene="adhE"
                     /locus_tag="VWX16_000476"
                     /gene_synonym="adhC"
     CDS             complement(5459..8110)
                     /gene="adhE"
                     /locus_tag="VWX16_000476"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9157887.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(8410..8820)
                     /locus_tag="VWX16_000477"
     CDS             complement(8410..8820)
                     /locus_tag="VWX16_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157888.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            complement(8822..9250)
                     /locus_tag="VWX16_000478"
     CDS             complement(8822..9250)
                     /locus_tag="VWX16_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9157889.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNVSDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            complement(9297..9596)
                     /gene="yajC"
                     /locus_tag="VWX16_000479"
     CDS             complement(9297..9596)
                     /gene="yajC"
                     /locus_tag="VWX16_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9157890.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            complement(9707..11689)
                     /gene="tkt"
                     /locus_tag="VWX16_000480"
     CDS             complement(9707..11689)
                     /gene="tkt"
                     /locus_tag="VWX16_000480"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9157891.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVAAAKEL
                     ASKGEKVRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLKQS"
     gene            complement(11803..12894)
                     /gene="ulaG"
                     /locus_tag="VWX16_000481"
     CDS             complement(11803..12894)
                     /gene="ulaG"
                     /locus_tag="VWX16_000481"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9157892.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            complement(13006..14679)
                     /locus_tag="VWX16_000482"
     CDS             complement(13006..14679)
                     /locus_tag="VWX16_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9157893.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVHNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTLSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            complement(14856..15560)
                     /locus_tag="VWX16_000483"
     CDS             complement(14856..15560)
                     /locus_tag="VWX16_000483"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9157894.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDFPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVEPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            complement(15562..16425)
                     /locus_tag="VWX16_000484"
     CDS             complement(15562..16425)
                     /locus_tag="VWX16_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9157895.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     GRLDWSKEERLEVVKAIYGTGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIATLHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            complement(16429..17094)
                     /locus_tag="VWX16_000485"
     CDS             complement(16429..17094)
                     /locus_tag="VWX16_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9157896.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVSQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            complement(17111..17596)
                     /locus_tag="VWX16_000486"
     CDS             complement(17111..17596)
                     /locus_tag="VWX16_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9157897.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            complement(17673..17954)
                     /locus_tag="VWX16_000487"
     CDS             complement(17673..17954)
                     /locus_tag="VWX16_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9157898.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            complement(17977..19434)
                     /locus_tag="VWX16_000488"
     CDS             complement(17977..19434)
                     /locus_tag="VWX16_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9157899.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            complement(19565..20188)
                     /locus_tag="VWX16_000489"
     CDS             complement(19565..20188)
                     /locus_tag="VWX16_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9157900.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            complement(20239..21225)
                     /locus_tag="VWX16_000490"
     CDS             complement(20239..21225)
                     /locus_tag="VWX16_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9157901.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            complement(21244..22068)
                     /locus_tag="VWX16_000491"
     CDS             complement(21244..22068)
                     /locus_tag="VWX16_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9157902.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNEVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGQFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            complement(22043..22414)
                     /gene="rnpA"
                     /locus_tag="VWX16_000492"
     CDS             complement(22043..22414)
                     /gene="rnpA"
                     /locus_tag="VWX16_000492"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9157903.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYQEGNGSEKETKVD"
     gene            complement(22563..23753)
                     /locus_tag="VWX16_000493"
     CDS             complement(22563..23753)
                     /locus_tag="VWX16_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9157904.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            complement(23804..24757)
                     /locus_tag="VWX16_000494"
     CDS             complement(23804..24757)
                     /locus_tag="VWX16_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9157905.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELEQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            complement(24818..25412)
                     /locus_tag="VWX16_000495"
                     /pseudo
     CDS             complement(24818..25412)
                     /locus_tag="VWX16_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(25549..25962)
                     /locus_tag="VWX16_000496"
     CDS             complement(25549..25962)
                     /locus_tag="VWX16_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9157906.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQVFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            complement(25940..26401)
                     /locus_tag="VWX16_000497"
     CDS             complement(25940..26401)
                     /locus_tag="VWX16_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9157907.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS"
     gene            complement(26364..26624)
                     /locus_tag="VWX16_000498"
     CDS             complement(26364..26624)
                     /locus_tag="VWX16_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157908.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(26629..27033)
                     /locus_tag="VWX16_000499"
     CDS             complement(26629..27033)
                     /locus_tag="VWX16_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9157909.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTNLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(27026..27352)
                     /gene="comGC"
                     /locus_tag="VWX16_000500"
     CDS             complement(27026..27352)
                     /gene="comGC"
                     /locus_tag="VWX16_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU9157910.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKEDGRITEEQAKAYKEYNDKNVGA
                     NRKVND"
     gene            complement(27354..28370)
                     /locus_tag="VWX16_000501"
     CDS             complement(27354..28370)
                     /locus_tag="VWX16_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9157911.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(28318..29259)
                     /locus_tag="VWX16_000502"
     CDS             complement(28318..29259)
                     /locus_tag="VWX16_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9157912.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(29335..29700)
                     /locus_tag="VWX16_000503"
     CDS             complement(29335..29700)
                     /locus_tag="VWX16_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9157913.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(29851..30909)
                     /locus_tag="VWX16_000504"
     CDS             complement(29851..30909)
                     /locus_tag="VWX16_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9157914.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(31072..32223)
                     /gene="nagA"
                     /locus_tag="VWX16_000505"
     CDS             complement(31072..32223)
                     /gene="nagA"
                     /locus_tag="VWX16_000505"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9157915.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(32376..34193)
                     /locus_tag="VWX16_000506"
     CDS             complement(32376..34193)
                     /locus_tag="VWX16_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9157916.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(34294..35436)
                     /gene="tgt"
                     /locus_tag="VWX16_000507"
     CDS             complement(34294..35436)
                     /gene="tgt"
                     /locus_tag="VWX16_000507"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9157917.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            35566..36423
                     /locus_tag="VWX16_000508"
     CDS             35566..36423
                     /locus_tag="VWX16_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9157918.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCHSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(36450..37067)
                     /locus_tag="VWX16_000509"
                     /pseudo
     CDS             complement(36450..37067)
                     /locus_tag="VWX16_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(37174..37533)
                     /locus_tag="VWX16_000510"
     CDS             complement(37174..37533)
                     /locus_tag="VWX16_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9157919.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            complement(37544..37969)
                     /locus_tag="VWX16_000511"
     CDS             complement(37544..37969)
                     /locus_tag="VWX16_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9157920.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(38292..39434)
                     /locus_tag="VWX16_000512"
     CDS             complement(38292..39434)
                     /locus_tag="VWX16_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9157921.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAEKQETQASPQAALAVEATTTS
                     SEAKEVASSNGATAAVSTYQSEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(39596..40219)
                     /locus_tag="VWX16_000513"
     CDS             complement(39596..40219)
                     /locus_tag="VWX16_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9157922.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFEAER"
     gene            complement(40223..41503)
                     /locus_tag="VWX16_000514"
     CDS             complement(40223..41503)
                     /locus_tag="VWX16_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9157923.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMILKG"
     gene            complement(41564..43048)
                     /locus_tag="VWX16_000515"
     CDS             complement(41564..43048)
                     /locus_tag="VWX16_000515"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9157924.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYELTDFDVEILELFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTTVAAADMQVAVEAYLGL"
     gene            complement(43124..44401)
                     /locus_tag="VWX16_000516"
     CDS             complement(43124..44401)
                     /locus_tag="VWX16_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9157925.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKFLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(44508..44591)
                     /locus_tag="VWX16_000517"
     tRNA            complement(44508..44591)
                     /locus_tag="VWX16_000517"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44555..44557),aa:Leu,seq:caa)
     gene            complement(44601..44672)
                     /locus_tag="VWX16_000518"
     tRNA            complement(44601..44672)
                     /locus_tag="VWX16_000518"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44638..44640),aa:Gln,seq:ttg)
     gene            complement(44686..44758)
                     /locus_tag="VWX16_000519"
     tRNA            complement(44686..44758)
                     /locus_tag="VWX16_000519"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44723..44725),aa:His,seq:gtg)
     gene            complement(44773..44843)
                     /locus_tag="VWX16_000520"
     tRNA            complement(44773..44843)
                     /locus_tag="VWX16_000520"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44809..44811),aa:Trp,seq:cca)
     gene            complement(44848..44928)
                     /locus_tag="VWX16_000521"
     tRNA            complement(44848..44928)
                     /locus_tag="VWX16_000521"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44892..44894),aa:Tyr,seq:gta)
     gene            complement(44941..45013)
                     /locus_tag="VWX16_000522"
     tRNA            complement(44941..45013)
                     /locus_tag="VWX16_000522"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44978..44980),aa:Phe,seq:gaa)
     gene            complement(45017..45090)
                     /locus_tag="VWX16_000523"
     tRNA            complement(45017..45090)
                     /locus_tag="VWX16_000523"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45054..45056),aa:Met,seq:cat)
     gene            complement(45101..45190)
                     /locus_tag="VWX16_000524"
     tRNA            complement(45101..45190)
                     /locus_tag="VWX16_000524"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45152..45154),aa:Ser,seq:tga)
     gene            complement(45273..45346)
                     /locus_tag="VWX16_000525"
     tRNA            complement(45273..45346)
                     /locus_tag="VWX16_000525"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45310..45312),aa:Ile,seq:gat)
     gene            complement(45381..45451)
                     /locus_tag="VWX16_000526"
     tRNA            complement(45381..45451)
                     /locus_tag="VWX16_000526"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45417..45419),aa:Gly,seq:tcc)
     gene            complement(45457..45529)
                     /locus_tag="VWX16_000527"
     tRNA            complement(45457..45529)
                     /locus_tag="VWX16_000527"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45494..45496),aa:Val,seq:tac)
BASE COUNT        13517 a         9982 c         8198 g        13834 t
ORIGIN      
        1 ataaattggg agattgaaat tcatttctag caatttttta gaagtcacag tctactattc
       61 taacctcagt atactatatg tgttacaaga aaagactcaa aaacttctcg tcagtgtaag
      121 aatgtttgaa acactattta aaactaaata actataagaa gtagatcagg tgaacaattt
      181 cagtacctac tctataagct atacgaagat atttttcaac atgatggata aaacgatcac
      241 tcttttaaag ccatcttatt tagaaaagag aaaccattta agcatagatg ggaccttctt
      301 gccctgtttt aaatttttct taaactagag tctgtaaaag attggcttca ttttcacatt
      361 ttctcctagg aggaatgtcg taatgcaaaa gaattaaatt caaataaatc atagcgatga
      421 cgactaatcg agtactcaaa cggggttccg ttatccaaat aagccacttg ttctacttca
      481 aataccggtt ccgttggaag gagctgcaag taatgttgct cattttctga actagaagaa
      541 gctcgcaaga tttttgtagc actggcaata tgcaatccta actttccttc aatgtaattg
      601 taaatcgatt tttgtaaaat atcttcagta atacctggaa taacatcggt actcatataa
      661 gtttgttcca gaacataagg tttaccgtca ataatacgca ggcggtaaat attataaact
      721 ggactctgca aagcgacctg tagtttttct gctaaaaatt catttgcaaa ttctaatttg
      781 aagtgaataa tcctcgagtc aattgtagaa tgagcatctt cagatatttt tgtcagtccc
      841 cggatatctc tttctggaac gattaatttt ctttttgagc tgccacgaga gagaacaaag
      901 gttccctgcc cacgttttct aaaaattaag ccctcagaaa ctaacaagtc taaagctttt
      961 ttgatcgtca ttcgactaca ggcaaactgt ttagctaatg tcatttcatc aggcaattgt
     1021 tcgttcatgg tatatttatt acaaagaatc ttttcacgaa tctcattgta aataaataaa
     1081 tattttgtac tcatttaatc tggttctctc tataatattc taatagtagt ataacaaagt
     1141 tcaagaaatg aggcaaacac aagatatgta tcaacaattt ttaaaaatgt ctagacaaat
     1201 tttaaaatta tgctatactt tatgtataga ataatagaaa ggaggttgag catgaaaaaa
     1261 aagttagtat ttcctaatct gttttggtgg ggagctgctt ctagcggacc tcagacagaa
     1321 ggtcaatatg gaaaagtaca tgaaaatgtg atggactact ggttcaaaac gcatccagaa
     1381 gattttttcg ataatgtcgg acctcttgta gccagtaact tttttcatac ttacaccgaa
     1441 gatttccact tgatgaagga aattggagtt aattctttcc gcacttccat ccaatggagt
     1501 cgactcatca agaatttaga gacaggtgag cctgatccaa aaggtattgc tttctacaat
     1561 gccatcattg aagaagctaa aaagaaccag atggatcttg tgatgaattt acatcatttt
     1621 gatttaccag tggaacttct tcaaaaatac ggtggttggg aaagcaaaca tgtagtggag
     1681 ttattcgtga agtttgccaa gactgcttta acatgctttg gagataaggt tcattactgg
     1741 acaactttca atgagccaat ggtcattcca gaagcaggat acttatatgc tttccattat
     1801 ccaaatctaa aaggaaaggg aaaagaggcc gtacaagtca tctataatct aaaccttgct
     1861 agtgcaaaag tgattcaact atatcgctca ttaggacttg atggaaagat tgggattatt
     1921 ttaaacttga cacctgctta tccaagaagt aattctccag aagacttaga agcaagtcga
     1981 tttacagatg acttctttaa caaagtcttc ttgaatccag ctgttaaagg aactttccca
     2041 gaaagattgg taaaacagct agagagagat ggcgtgttat ggagtcatac cgaaaaagag
     2101 cttcaactga tgaaatcaaa tacggttgat tttcttggag taaactacta ccatccaaaa
     2161 cgtgttcaag cacaagcaaa tcctgaggaa tatcagacgc cctggatgcc agaccaatac
     2221 ttcaaagagt atgaatggcc ggagcgtcgc atgaatccat atcgtggttg ggaaattttt
     2281 ccgaaagcca tttatgatat tgctatgatt gtgaaggaag aatatgataa tattccatgg
     2341 tttatcagtg aaaacggaat gggtgttgaa aacgaagcac ggtttatcgg tgaaaatgga
     2401 gttatcgatg acgtgtatcg tattgaattt tatgaagaac atttaagatg gctacataaa
     2461 gccattgaag agggaagtca ctgttttgga taccacgctt ggaccgcatt tgattgctgg
     2521 tcttggaata atgcatataa gaatcgttac ggatttatct ccgttgattt agaaacgcaa
     2581 aagagaacca tcaagagctc aggaagatgg tatcgcaaag taagtgacaa taacggtttt
     2641 gaagtagaaa ttgaggagta aagagaggag atattcagat gaaaaactca aaatttatag
     2701 atcaatttgc cacctttgct ggtaaactag ggaaccaaat tcatttaaaa actctaagag
     2761 atgcattcgt aacagtaatg ccattatata ttttggcagg tttaatcgtt cttttgaaca
     2821 acacggtatt taagtggatt ttccaagggg atacattaac aagattccaa tattggggaa
     2881 taacaattgc aaacggtact ttaagtattt caggtatgat tattgctgta atggttggtt
     2941 atttcttagc taaaaacaga gatttcgaaa acccgttagc agcatcaatg ctatcattag
     3001 tttctttaat tgtgatgatg ccaaatacag tttctgtagt tcctgacgga gcaaaagatg
     3061 ctgtaaacat ttcaggtgtt ctttcattca acaacacagg tacaggcgca atgttcgccg
     3121 gggttatcgt agcgattatt gcaacagaat tattcattga attatcaaat gttaaagctt
     3181 tacaaatgaa ccttggtgaa aatattccac cagctgttag tagatcattt agcgtattac
     3241 ttccagtcat gaccgtcatc tccttatttg gggttgtttc agcattatta ttcaatataa
     3301 ctggaatgaa cttaatctca atcattacaa tctttattca agaaccaatt cgtcatattg
     3361 gtacaagctt aatcggggtc attattattt actctttagg aaatatgtta tggctatttg
     3421 gtattcacca agcagttatt tacagtgcca tcctagaacc attactatta attaacatta
     3481 ctgaaaacat cactgcagca aataatggac aagccattcc acacatcatc aacctatcac
     3541 aaatacaaac attcgcttta atgggtggta gtggatctac attatgttta ttaatagcaa
     3601 cattcttagt aagtcgcaat gctgtttcta aaaacgtggc taaattatct tttggacctg
     3661 gtatcttcaa tatcaatgaa ccagtattat tcggttaccc aatcgtttat aacatttcat
     3721 tagctattcc atttatcaca gttccagtcc ttggtatttt aatcagctac ttagcaacag
     3781 ttacaggatt catgagtcct gcatttatac aagttccttg gactacacca gtattcttaa
     3841 atgcatggtt agcaacagca ggggacgtga gagcagttct agttcaattc atcatctttg
     3901 cacttggagt tcttctatac attccattta tcaaagttaa tgacaaagtt gttgaacaag
     3961 aaatggaagg ttaagattat gaaacaaatt ttgttagtat gtaatgcggg aatgtcgaca
     4021 agtatgcttg ttaaaaaaat gcaacaaagt gcgactgaac gtgggattga aatttctatc
     4081 caagcaaaat caatgacaga agctaaaaag aatatccatg aagcagatgt cattctaatt
     4141 gggccacaaa ttcgttatga gttacttgct gtgaaagaaa ttgcaggaaa tatccctgta
     4201 gataccattg atatgcggga ttacggaatg atgaatggag caaaagttct ggaacaagca
     4261 cttgaatgga taggagaaat aagatgacag aagaaatgga atatatctgt tttcagctca
     4321 ttgccaatag tggagcagcc aaatcatcct ttattgaagc catccaacta gctaaggcag
     4381 gaaatttaaa agaagcgaaa attaaagttg aagaagcgga agactcctta gtagaagcgc
     4441 acaagattca ctctaatctc atacaaaaag aagctactgg agaaaaaata ggattctcct
     4501 tgctatttat gcatgcagaa gaccaaatgg cttcaacaga gattattcag ttgctatcta
     4561 aagaattcat cgatctctat caaaataaat aggaacactg tcattcccat ctgtctttat
     4621 taatgacgac tacattgtag gaacctccaa aagttggaca aaatgattga aaggattgga
     4681 tcctgcatta cacaagatcc aatccttttc ttttactcta gtttttataa tatgttgaat
     4741 ctagaatagt ccactgtagt ttctaaaaaa ttgctggaaa tggatgttaa gctccaattt
     4801 atttgtttat atcttatttc agtttactat actagcttat tattctagtg aaaaattgaa
     4861 tgagccacta tttatttaat atgtatgatg agggaatgac tcaaaaactt cttgtcgata
     4921 taagaatatc tgagatagta ttttaaaacg aaaggactat aaaagctcat ttccttcgca
     4981 cttattttat agagatctat cattatactc tttagaaatc aaaaagcaac acaccttgtc
     5041 atcgttttat cgtaaatctg ttaatgaggg ggctagtctt atctgcaatc tcaaatcagt
     5101 gttttgagct gaatggcact cgttttctag tttgctcttt gattttaatt gggccttagt
     5161 aaatactact cttaaattaa actatagtga attgaaataa gatgtgaaca actctatcag
     5221 gaaagtaaaa ttaatttcta taaatgtttt agaagcagat gtgtactatt ctagtttcaa
     5281 tctattacat attttgtttc tattctagtt tttcaaaaag ggcaaaaagg cctcaatatc
     5341 ataaaaaggc atcctatcag gtgttcaaaa accttgatag gatgcctttt atcatagaaa
     5401 aattactgga tctttccctc aaattgagtt tttgtcttgc ttctagactg ataaacaatt
     5461 atttacggcg tcctggtctt tctttgtagc catagtatgc atcttcgatg atttcttgca
     5521 tatggtctac cattggaaga cgtgggttag ctggtgaaca ttggtcttca taagccagga
     5581 aggctaattc acgagaatgt tctttccatt ctttttcgtc aattccttgg tctctaaaat
     5641 tcatttggat cccaatacgt tcaccgagtt cgtagacagc ttttgcgtaa gattcaaccc
     5701 cttcttctgg agtagaagct ggaagtccaa gcatgcgtgc gatatcttgg tatttttcat
     5761 ctgcacggta gtagttgtac ttaggccatg ttgctgtctt agctggacgt gtaccgttgt
     5821 aacggataac gtatggaagc aagatagcat ttgtacgacc gtggattgtg tggaattgcg
     5881 caccaatctt atgggccatt gagtgagaaa tacctaggaa ggcattggca aaggccatac
     5941 cagcgattgt tgaagcgtta tgcattttct cacgtgagtg gaagtcagca ttcttaactg
     6001 agctttcgag attttcaaag accaatttaa tggcttgaag tgctaaacca tcagtgtagt
     6061 cactagccat ttgtgatacg tatgcttctg tcgcgtgagt caatacgtcc ataccagtat
     6121 cagcagcaac aaatcctgga actgtcaata ccaaagcagg atctacgatt gccacagttg
     6181 gtgtcaatga gtagtcagcg attgggtatt tacggttgtt tgctttatca gagataacgg
     6241 caaatggtgt tacttcagat cctgtaccag atgtagttgg aatcgcgatg aatttagtct
     6301 tcttaccaag caatgggaac ttgaaggcac gtttacggat atccatgaat ttttggacaa
     6361 ggtcacggaa gtccacttct ggttgctcgt agaagagcca cattactttg gcagcatcca
     6421 ttggagaccc accaccgagt gcgatgatgg tatctggttt gaaggcacgc ataatctcag
     6481 taccacggtt tacagttgtg atatctggat ccggttctac atccgcaaag atttggtaaa
     6541 caaccttatt gcgacgaagg tccagttgtt cgatgatacg atcaaggaaa ccaagctcta
     6601 ccatggcatg gtcagtaacg atcatgacac gttcaacgtc acgacatttt tgaaggtatt
     6661 gaattgaatc acgttcaaag tatgtttttg aaggaagttt catccattgc atgttatttc
     6721 tccgtcttcc gacttttttg atattcaaga ggttaatggc actaacgtta tccccaactg
     6781 agttgcgtcc gtaagaacca catccaagtg tcaatgatgg caagaaggca ttgtaaacgt
     6841 ccccgatacc accaaaagta gaaggtgagt tacagataac acgaatagct ttaacagctt
     6901 taccaaattc tttagtcaat tcttcgtcag ctgtgtggat agctgctgag tgtccaagac
     6961 cgttaaattc aaccatttga cgagccttag taataccatc ttcacggctt tcagatttca
     7021 aaactgcaat aactggtgac aatttttcac gagtcaatgg ctcattttcg ccaacttctt
     7081 tacattctgc agcaagaatg tttgttcctt ctggaactgt aaatcctgct tgttctgcaa
     7141 tccaagttgc tggtttacca acgatgtcag cgttcaattt tgcaccagca cagtttttgc
     7201 tgtttgcttt gacgccgaag cagaactctt caagaagagc tttttctttt ttgtttacaa
     7261 agtaagtgtg gtaagatttg aactctgcta caaattcatc gtaaatttct ttatcaatga
     7321 taactgcttg ttcagatgca cagaccatac cgttatcaaa tgatttagac atgacgatat
     7381 cgtgtgctgc ttgacgaatg tttgctgatt tttcaacata agctggaacg tttccggcac
     7441 ctaccccaag agctggttta ccacatgaat aagccgcctt aaccatggca ttaccacctg
     7501 ttgcaaggat tgtcgcaaca ccttcgtggt tcataagggc acttgttgct tccatagatg
     7561 gttgagtaat ccattgcaca cagttttcag gagcaccagc tgcgatagct gcatcgcgga
     7621 cgatacgagc tgcatgagca gatgattctt gtgctgatgg atggaaggca aagacgattg
     7681 ggttacgtgt cttcaatgaa atcaatgatt tgaagattgc tgttgatgtt gggtttgttg
     7741 ttggagtaat accacaaaca acaccaactg gttcagcaat aagagtcaat cctgttacat
     7801 cgtcttcttc gataacgcca actgtcttag tgtggcgcat gttgtttact acgtgttcac
     7861 aggcaaacaa gttcttagtt gctttatctt caaatacacc acgtcctgtt tcttcaaagg
     7921 catgtaaagc caattctccg tgggcatcca aagctgctac tgatgctttg gcaacgatgt
     7981 agtcaacttg ctcttgatcc aatttacgca tttcttcaag ggcaactaga gctttttgaa
     8041 ccaactcatc tacgtgtttt tcagcaacga gtttcttttc ctctggtgtc acagtttttt
     8101 tatcagccat attttcctcc atagctttca aggaacgata tcctttgtta attttttcac
     8161 aagtttatta taacgcatta tttcaacttt gtaaacagtt tcataaacat ttcacaaaga
     8221 atttttaaac tcttatgaaa cttttctcat tttcttttct tatcaggttt tatctttgaa
     8281 gtttgtgaaa tttatttcaa atatttttta tttcacaaac ttgcttggaa gagggatttg
     8341 gaaagaaagg gatttacagg taaacgctta ccttgtaatt ctaataatac ctcctttgat
     8401 aaggaggctt tatttttgtt gaaaaacgcc ttgtttaaaa gttccttcat aaacgacttc
     8461 ttgttctgtt gttagtttcc cttttccttc agcctgacca tttacaaaat caccttcgta
     8521 ggtccagcct tctttagatt gaaaggtacc ttttccgttg aaggctccat tgttgaagcc
     8581 acctgtatat tggtctccat tttggaaggt aatggtacct tggccattca ttttaccacg
     8641 gacaagactg ccatcataaa caatcgttcc attatcgagt tttaagacac cttttcctgg
     8701 aatatttaga aaaaagacga gtacagcaca gaaaacaatg gtaactactg ccaaaagctc
     8761 taaacgtgga cgagtcagat agacacgata cttttcgtaa aaattcgtaa gattttccac
     8821 cttaactctc cttttctaaa cgttctaacc aagcttgaca gccctcttga acacgtcgat
     8881 aggtttcttc aaaatctcct gtataccaag ggtctggaac actttcagat gaaaatgagt
     8941 aaatcttatc ttgacagtct actggacaca tctgacgtaa gtcggaaaca tttgaagcgt
     9001 ccattccgat aatataatca aaggcttcaa aatcttcctt actaatctga agcgatgtct
     9061 tgttcttgtc ataaggaatc tcatactctt gaaaaattcc ctgagtcccc ttatgaatcg
     9121 gattgccatg ttcccaagag gaagttgctc gactttggat ttcgtagtta tctgtcattg
     9181 atttcataac aaactcggcc atagggctac ggcaaatatt tcccagacag acaaagacta
     9241 attttttcat cccatttcct ttcttttata gaaaaacggg agttcgtgat cccgtcttat
     9301 ttttcaattg cgccttctag tgaagctgtt tctttcagcg gtaatactgt cttgatagca
     9361 gctagttcaa aagtcaagta aactccatct acgtcaagaa ctattgttcc cttctccgta
     9421 tctacttcat cgactgttcc ataaagtcca ccgattgtaa tcacttcata gcctttttgt
     9481 agtttattta agctttccat acgtttttgt gcttgtttct tttgagagcg ttgcataaag
     9541 aacatcaagg ccatcatacc tacaagcatg attaaaaaag taatatttgg attcattgtt
     9601 ttctcctttg tcttttacat aggactacta tatcaaattt cagctacttt tacaagattg
     9661 taccttactt ttctggtaag aaaaaccaga gcttacgccc tgatttttag gattgtttca
     9721 agtttcgaac aacttttaca agattttcta cagtaaagcc atattctgcc aatacttttg
     9781 gtgctggggc agaggctccg aaggtatcaa tacctagaac ggcaccatcg agaccaacat
     9841 atttgtacca gttttgactt gcacccattt cgactgcaac acgacggcgg actgcatttg
     9901 gaagaatctc ttccttgtaa gctgcatctt gtttatcaaa gacatctgta gatggcatgc
     9961 tgactacgcg gactttttcg cctttactag ctaattcttt ggcagctgcg acagctagat
    10021 tgacctctga acctgtcgca atcaagatgg tatcaaagtc ggctgcattt tcatatacaa
    10081 cataagcacc tttagcaacc ttgtcgaagt ctgttccatc ttcaacagtc aagttttgac
    10141 gtgtcaagac aagggcagtt ggtgttttct cacttgtcac tgcaaggtac caagctgctt
    10201 gcgtttcacg cgcatctgct ggacggaaaa catttagatt tggcatagca cgaagacctg
    10261 ctaaatgctc aactggttcg tgagtcggac catcttcccc aactgcgatt gaatcatggg
    10321 taaagacata agtcacagga agtccttgta aggctgacaa gcggacagct gccttcacat
    10381 agtcagagaa gacgaagaaa gttccaccgt atacacgaag tccaccatga agggccatcc
    10441 cgttcaagat tgttcccatt gcaaattcac gaacaccaaa ctgaatgtta cgattcaagc
    10501 gattggtatc gtcttgaagt ccatccgtct tgatataagt catgtttgag tgagcaaggt
    10561 cagctgatcc acctaggaag gttggtaatt tagctgccac aacattcaag gcatcttgac
    10621 ttgaattacg agttgcttga gaaaaaccat tttctaaagc tgggaagtct gctggagtca
    10681 cttcgactgg atcacgtccg tcgatgatgg cctctacttc tgcagccagt tctggatgag
    10741 cttctttata atctgcaact aatttagtcc aagcttgata agctgatgcg ccacggtctg
    10801 caacatgttc tttgaaatca gcatatactt gttctggaat ttcaaatggt tcgtagtccc
    10861 aaccgagggc ttggcgagtt gatgcagttt catctgctcc aagaggggcg ccgtgtacag
    10921 cattagttcc ttgtttgttt ggagaaccgt atccaataac cgtcttcact tcaatcaaag
    10981 atggcttgcc tgaagctttt gctgtttcga tagcagcatg gatggcttcc aagtctgttc
    11041 cattttcaac caaggcagta tgccaaccgt aggcattgta acggtcacga acactttctg
    11101 taaaggaatc ctttgtctca ccatccaagt tgatatcatt tgaatcataa agaacaacca
    11161 acttatcaag tttttgcaag cctgcgtatg aagctgcctc gcttgagaca ccttccatca
    11221 agtctccgtc tccacagata acgtaagtat agtggtcaaa gatattgtag ccttcacggt
    11281 tatatttggc tgccaagaaa cgttctgctt gggcaaaacc agtagcagtt gaaatccctt
    11341 gccctagagg acctgtcgta gcatcaatcc ctgccgtatg accaaattct gggtgacctg
    11401 gtgtttttga accccattga cggaagctct taatctcatc catgctgaca tcttcaaaac
    11461 cagaaaggtg aagaagagca taaaggagca ttgaaccatg acctgctgaa agaataaagc
    11521 ggtcgcggtt aatccagttt ggttgagctg gattgatatg aagttgtttt gtaaagaggc
    11581 tgtaagccat cggagccgct cccataacca cacctggatg acctgagttg gctttattaa
    11641 tggcgtcaat acctagaaaa cgaattgcat taacagatag atttgacata gaatttcctt
    11701 tctatttgag gtgattattg aatcacacat tctattttac tattatatga aaattatccg
    11761 tatcatgcaa catacggata acctccaaag aatattttta tattatagca aagctttaaa
    11821 ttgaatgtta gagtcttgtt caaaacaatc atcaaaacca cgtggatgat ggtattctac
    11881 taagtgttga tcttgaggat aagtgtactt accgccaact tcccagataa atggatggaa
    11941 atcgtattgc aagcgatctt ttcgcatttt ccaaagttct agaatctcat tagtagaagc
    12001 catgaagtta gaccagatat catagtgaac tgggataatg actttggtac gcagattttc
    12061 tgccatacga agaaggtcga tagatgtcat tttgtcttgg atacctaccg gattttcacc
    12121 atagttattc aaagcaacat caattttaaa gtctttacca tgttttgcaa aatagtttga
    12181 gaagtgagaa tctgcaccat gatagatggt tccacctggt gtttcaaaga tatagttaac
    12241 agccttttga gccatttctt catctgtaac agccaagcca gcaagttcac cgcctgtctc
    12301 atcagcaccg ttcactggga gagttaccaa gcaagtacgg tcaaatgatt ctactgcatg
    12361 aatcttcata tcttttaatt cgatagtgtc acctggttta acaacgatga tacgttcttt
    12421 tggaacaccc catccttccc agattcgtcc acagtggtaa ggaccgataa acttaacatg
    12481 ctctaactta ggattgttga gaattgctgc agctgtgtat gggtcgatat gatcactgtg
    12541 gaagtgtgaa actaagtaat agtctagttc gttgatagca aatggatcga taaccattgg
    12601 ctgaacacgc aagtttggtt gcagcttacg aacacctgcc atatttgcca tttggtgccc
    12661 acgaaccata tctttcactt ttttggttga ttttccacgg tttgaccaaa ggtccatgac
    12721 aacgttagca ccagctggtg tcttaatcca agtaccacag ttgcctagcc accacatggc
    12781 aaagttgcct tcaggtacga cttcttcttc gatttcttcg ttcaaccatg ttccccactc
    12841 tgggaaagtg gctaaaatcc atgactctct tgtaatttct ttgacgtttg gcatatgttg
    12901 cctcctttgg atataattgt tatctttgag attagtatac cacccttcaa aacactaaaa
    12961 aagaccaaga aaaacacagg tttgtgttca tcattggact gtccttcaaa tatcttccaa
    13021 aaaagcttga actactattt cttgatttat aagtgtttga acctcttctt ggaacttact
    13081 agccaacttg ctatctacaa tataagacga aataagacta gaaagatttt cactgaaact
    13141 tttgctctca ttacgaaata tattgtgttt aagatagtct agcattttca aaatatcttc
    13201 tgcttcaagg ataggattaa cccttaaaat cggaaatctg ctatccaaat catcattagt
    13261 agtaatcact acatcaactt gtgcaatatc ttccacactc ttaaattgtt ctgttgtaaa
    13321 tacagtgtca atttgctcat ttggaaaata aagtttgcat tgtttcagca aaagtctcga
    13381 aaccgcaaca ccttcatcac aaacgagata aacttttttc atattttttt gagacgatag
    13441 tgtatacttt aaaaatcctc caatatgaat cgtcaaatag gcaatatcat cgtctgttag
    13501 ccgaataaac catgctcctt ctaaaatttc cgcagatttt ctagtgacta aaaataattc
    13561 tccatacttg gatcgaattt gttttgttag aggattttta gaaaaaatac cgtaagtctt
    13621 tctaaataac aaggctttac agtggatcat caaatttcgt aacaaatcat ccttgttctc
    13681 aatctccatt cggatttgtg attcaaaata ccagataaat tcttctaaag caagttttaa
    13741 ttgccgaaaa tcatcacttt ctgcatgaat atccaaatct ttacgatagg agaggaggag
    13801 aacagctaca agagaaactt caagtcctga caaagaaatt tcaaactttt gaaacaaccg
    13861 ttctcctaat ttcttagaca cctgatactc tattcttttt cttatcaaag aaaattcttt
    13921 ctctatatcc tgatgtcttt cttgatactg ttcaacatta tgacagctta acagcaaata
    13981 aggtagaacc tgcaacataa aagttatttc atgatggttt attttcttcc ctaaatcttg
    14041 ttcaactaaa ggaacctgtt ccttaaaaaa ttggttcatt tcaacagaaa gcaaacactc
    14101 atcatctaac ctctctttca tcttatcttc taaaatagat acaaaagtgg cattttcttc
    14161 cataaaaata tgatatagaa gcgattgaag atactgaatt ttattaagag ggtgggcatg
    14221 aaggtggtat ccctgtgact tgctcacttg caaggtcacc tgatattgtt ccaaagttag
    14281 ttgataacga atactattca aatcattgtg aacagtattc ctagatacct ctgttaactc
    14341 tatcaatttt tcaatagtaa tacgttcttt agaaatacct atccatagta acattatcat
    14401 catacgttca tgcgcactca tgatataatc gtacgaatct acttccgata atagtttaca
    14461 acaagcatct ctctgctctt ccgttaagtg aataccaatt cgtggaatac taatgatgtg
    14521 aagcgcctcg tcaaccagag cagcattgat tttgtcaatg tgataataaa ttttccttct
    14581 tgactctttc aaatctttcg aaatcgccat aatcgttttg gacgtttctt gatccatcaa
    14641 atattgaagc aaatcacaac ttgccttatc cagtaccata ttaaaaaatc ccccaaatct
    14701 attgatatca cagcaattct ttttatttta ccatatatat caagcgctat ttctaaaaaa
    14761 tctttcaaaa ttacttcaac tccaacttta ctctcaataa aaatcaaatg taaaaagagg
    14821 agctaaattt atctttttct cctccttcat cgttcttact tttgaccata ataagcattt
    14881 ggtccatgtt tacgttggta gtgtttttct agtatgtact ggggagcagg ttcaactctt
    14941 ggattgattt gttctgtaaa gcgattcatc tttgatactt cctctagtac gacagagtga
    15001 taaacagcat tctctggatt tttgccccag gtgaatggac cgtgattgcg tacaacaatt
    15061 cctggtactt caaccgggtt aagtccgcga tgttcaaact cttctacgat aaccaggcca
    15121 gtatcttttt cataggccac ttctacttcg tccttggtca aactacgggc gcaagggatt
    15181 gaaccgtaga aataatctgc atgggttgtt ccgtagaaag gaatatcacg acctgcctga
    15241 gcccaagcaa cagcttctgt cgaatgggtg tgaaccacac taccaatttc tgaccaagcc
    15301 ttatataatt gcacatgagt tgggaagtcg gaagatggtc ttaaatcccc ttctaggatc
    15361 ttaccatcta gatcagtcac taccatgttt tcaggtgtca attcgtcata atccacgcct
    15421 gatggtttga taacaatgac accgagttcg cgattgactt cagatacatt cccccaggta
    15481 aatttgacaa gtccatgttt tggcaatgat tgattggcat cacagactcg tttacgcata
    15541 gcattgatta cttgattcat cttacatcaa acctgctttc ttaatgagtg gatagagaaa
    15601 agcttgcgcc tcttgaatgg ctgcgcgtgt ttcttctact gtttcacaat tttcagacca
    15661 catttcgatt aggaaaggtc cattataatt ggtttccttt aaaatatcga aagcttcttc
    15721 ccatttgaca caaccttgcc caaaaggtac atctcggaac tggccctttg aactttctgt
    15781 cactgcataa gtatccttga gatggagagt tgcgatggca tgatgaccaa gataaaactc
    15841 actatagata tcattatgcc atgcagacac attaccaata tctggatata caaagaggaa
    15901 gggagagtca atctcttttt ctatagccaa atatttttcg atgctattga tgaaaggatc
    15961 atccataatt tcaatagcaa gtaccacctg agcttcttca gcccagtcac aggcttttct
    16021 caaatttttg ataaaacgtt ggcgtgtctg gggtgacttt tcctcatagt aaacatcgta
    16081 accagctaat tgaatcgtac gaactcccaa gtcttgagct aattcgatac attttttcat
    16141 gagttctaga gatttttcct ctagaactgg atcttttgaa cccaatgggt agcgacgatg
    16201 gcctgaaaaa cagatagaag gaatacgaac accagttcca tagattgctt tgacaacttc
    16261 caagcgttct tccttactcc agtcaagtct tcctaaacgc tcgtcacgtt cgtcaataga
    16321 catctcgaca aaatcaaagc ctaactcctt ggcaaaattt aaacgttcta gccaagtaaa
    16381 gtgtgttggg gttgcctttt cataaattcc aattggacgt accatgattt acccccaaat
    16441 tcgtttgatt tcatccttga aggcacgcgc tgctcctgct ggatccacag cctctgtaat
    16501 tccacgacct gcgataaagg taaagacatc aacaccttca aagagtttga gagtatctac
    16561 atctagacca cctgttacag atacacggaa gcccatgtca atgagttttt taaccttatt
    16621 aaggtctttt tcaccccaag tttcaccagc aagaagagca tcacgagatt ggtgataaat
    16681 agcttgtgag atacctgcat ctagccaaag ctgagcttgt tcaaaagtcc aatcgccata
    16741 aagctcgatc tggatttcgc ctcgttctcc tcgttcagtc ttgatagcct ttagagctgc
    16801 ttccatagta gggatggttg cacaacagat acaagtcatc cagtctgctc cacgaaccgc
    16861 attattttta gcaactgttc caccagcatc agcacatttt gtgtctgcca caataatctt
    16921 atctgggaaa aggctacgca agacttcagc cagttcactt ccaacttgaa gcaagcaaac
    16981 agttccagct tcgataatat ctacttcctg actaacagaa acagctgctt taatcgctcc
    17041 ttgcaagtct gaatggtcta atgcaacttg taaattaggt attctttttg tcatttcttt
    17101 attcctcttt ctaactttcc aaatccaatc cttcgagata agggctatcc ttagattctt
    17161 caatcatagc caagacatct tctttagtct ggcaagcctg taaacgtgca atagaatctt
    17221 ctaattcaaa cagggcaata atttgtggaa tggctacact tgtgtgaatt tttgagcttg
    17281 ttgctgctag tgccaacaaa acagatacct ctttcccatc tgaaaataca acaggatttt
    17341 gtaaggtaat caatgaaaag gcatcacttt gcacaccagc ttcaggtcta gcgtggggca
    17401 tagccatacc tggcatcaag atatagtaag gcccatactc ttcagtcgat tcaatgatag
    17461 catcgtaata ctctggcaaa attgccccac tttcaatcaa gggatctact gctaccttga
    17521 ctgcttcttt ccaatcgtta gcctctaaac ctagtcggat cgagtcgttg tcaattaaag
    17581 cttgttttaa attcatatct gcctccttta tttaaagagg gagaaggaca gaaacgataa
    17641 ctctcagttt ctgtccagcc ccctccaacc ttttactgta gtgcttgact gagtttttcg
    17701 gtgatttctt tatcatccat caagttatca agcccaatta acttcccatt agttcgccct
    17761 tccaattctt gaatcaaatg aagagaagcg attacgatgt catatcctac tgctaaacct
    17821 ttagcttcac cgacactgca tgaattgact gtaaaatctg tttgattaag cttacggaga
    17881 gcattttcaa ccttcatctt gataaccatt gatgaaccca ttccatttcc gcacgctgct
    17941 aatactttaa ccattttatt tctccttttc tagatactaa gcttcttctt gaacttcacc
    18001 gttgtaatat ttctctttat cttttgcttt ggcaaattga agttgaggaa taacaagcaa
    18061 gaagagacac acaagtacat aaccaacaat accaaggtat ttgaagatat atccaaatcc
    18121 aagccatggg aattcaaagt cgatatttcc atggtagcca ccataagatg ccaaatcgag
    18181 aagggccaca caaagagctc ctagagcaac ttgaaggaca cctgatataa aggaaaggat
    18241 aacagccgct ttccatccgc cgcgtttatc agcgtagacc gcaatggctg cattgtcaaa
    18301 gaacactggt acaaatcctg taataataag aatcggattt ttaaagacga tgagcaagac
    18361 aattgtaatc aattgaccaa tcaaaccaaa ggtaaatcct gacaagacag catttggaga
    18421 accaaatcca taagaagctg caacgtcaac cgctgggaat gaacctggca acaatttgtt
    18481 tgaaatacct tggaaggcgt ttgtcaactc agatacgaac attcggacac cttgcatcaa
    18541 aacgaacaag taaactgaga aggtaaaggc tgtttggata atgtacataa agaaatcttg
    18601 tttagcagga ttgaatagag ttcctgaagt gatgacttct ttattagaca taatgtctgg
    18661 acccaagatt aaaagaatgg ccccgaagaa tacgagcatc aaggtagcag atgcaacaac
    18721 tgtatcgtgg aagattgaga ggaacttagg taatttaaga ttgtctaaac tttcttcttt
    18781 cttaccaaag cgtcctgcta ctttatctac aaaccagatt gcaaattgct gttggtgacc
    18841 aatcgcaaat ccgccaccac cagtcaagcg ttgagttgcc tcaacagtca tatttgaact
    18901 aactgcccag taaagtccac agatgatacc aatcgctgct gtaccgtaag cattgcgcaa
    18961 ttgtggtact aagaatagaa ccataagaga tactgttgca gcttgttgta ccatgatgtg
    19021 accagtaata aagagggttc ttaccttcgt aatctttcga agagctacga gcaagatatt
    19081 tattccaaaa ccaatcaata gagctgtagt tgcagttcca acaaaatctg gaaactctgc
    19141 tgcaattttg ttgtttgctg cagcaagtcc aaagtaaggg tcgataaccg ctgcaccaat
    19201 ttggaatttg tagttaagag ctgctaagat tggacgaaag gttgtaacca aaccaccagc
    19261 acccacgtta agcaacatat accctactgt tgctttaaca aaccctgaaa aaacgtcatg
    19321 ggcaggtttt ttcaaaagtg catatcctat caacaccaat aaacctacga aaaatgcggg
    19381 attctgcaaa atattgctag aaaaccaatt tagaacactt gaaacgactt ccataattgt
    19441 tctccttttg aattgttttt gtaagtttat tatataatgc gaaagcgcaa acaaaaagac
    19501 caagaattac acagcggact gttcactctt ggactgtatg atttatgatg ttttacaaat
    19561 tatcttattt tctcgtagct agataatagg gaagaatttt agaataagac ttcaataagc
    19621 cttctacgat ttcttcttca gacgaatttt ctgtcatagg aacatctacc ttcactaaaa
    19681 cttttcgaac ttcttgactt ctcaccttct cgcgtaaagt acgacgcttt tcttcattcg
    19741 cctcccaccg ttgactttga ccattagagt aggttagata ataaatccct ttaacggttg
    19801 gaatgtctaa aactttggcc tgcttgccca gtgtttgctc atccttctta cgttcgatga
    19861 aactgacttc caaagaaatt ccaaagtcag tagatgtccc atacaaacgt aaggccatca
    19921 taggttctgt cacttgtccg tctctctgta gataaaccca aaaatgtggt ctcaaacgct
    19981 gggcctgatt catccactga ctagtctgtt ggagttgcca ttctggatgg cttgcttgaa
    20041 aggctttggc cagttctgta aaagcctttc gggcctcttg tcctttgtgg cggaatgcca
    20101 gcatcttctc tcgctctgct cccgctttat caggattacg gtactgcaaa ccagcaaagt
    20161 ctaaatagtc tcttatctta ttcaacatca gtttaacctc ctctagctag caaaaaatca
    20221 ggtcaaccct gattttactt attctgtatc tacaacgaca tagcgatttg gctcatcacc
    20281 ttcagagtaa ctggtcacgc catccatacg tgaaataata cggtggataa tcttgcgttc
    20341 actattcgac attggatctg tttgatgact acgtccttct tctaaaacac gaatcgccaa
    20401 tttttgcgca taggtctgca agacttctgc acggtgttcg acataatcat tgacattgat
    20461 tgtaacgtag aaggttttgg aatagcggtt gtaaagataa ttttgagcca acagttgcaa
    20521 ggccttcaag actttaccat ggtagccgat aatacgacct ggttcgttgg tgtcaatttg
    20581 tagattgatg ctacgacggt tataatcatt tgaaagtgta gcttcaacat ccatgtcatc
    20641 cacaatcgct tgaacatagg cagtcacatc cgtagccacc tgggcaatat cataactttg
    20701 ctcgaccttc aagcccatct ctttcaagtc ttgattttca gtttgatctt gctcagtttc
    20761 aaggtcatca gcgcctgctt cttccctcat cgcttcctcg atttgaagtt catttaaaat
    20821 ctcaatgtga ccagtttctt ctaagatagt gctggcatgt ctttcatgtt ttaagatttc
    20881 agccttgact tcatcagaaa taccttgacc ttcttcctct atttttttaa tagcatcaac
    20941 cacatgacca aggtcaacgg tttcttcact aaccgtcttc acaggctcgt tcaaatcatt
    21001 gatttttttc ggaacgcctt ttactacctg ttgatttgct ttgacaacag tcgtttcact
    21061 aatcgcttca atatccactt gggctggttt tttaccaaat agaccaagaa agcctttttt
    21121 ctccctagaa atgactttga tatgagcctt cattcttgga atatctaatt ctttcaatcc
    21181 tttctggatt gcttcttcaa cagttgaacc tgtaaatact accattacca gattcctcct
    21241 tatttatttc gttttctgag cctttttctt ggcttttctt tttctatttt ccaaatcttt
    21301 ttgtgcctgt actacggcct cgcgctctgc gataatcttg aatggattat tcaagaaata
    21361 ggtttgcaag acttgataag cattagacac tgtccagtat agggcgactc cacctggcgc
    21421 ataaactgca aagataaaaa tcaagactgg aatcccatac atcatcgcag tcgtagctcc
    21481 atttcgctca gacaaagctt tgttggacaa ccaagtactt aaaaaggtga atactgctgc
    21541 taaaatcgga agaacaaggg ttgtatccac actaccaagg ttaatccata agaactgacc
    21601 tgtctttaaa aagtcaactc ttgatagggc ttggaacagg gccaaaataa ccggcatctg
    21661 aatcaaaatc ggccaaagag agtctgactg tctgacaccc atttctttaa atactttacg
    21721 catttcctgc tctagtttgg ttctgctttc catatctcga cctggatatt gttctcgaag
    21781 cgccttaatg cgtggctgag cttcctgcat tttcctagaa gccaccattt gcacctgaaa
    21841 gactggcaag aggactgtac gaatcaagac cgtaaagaga ataatcccca ctccgatact
    21901 aatatcaaac gataaaaagc gaatgatttc cgcaaagaag taaaccaatt tactccaaaa
    21961 atcagccgat tcggctgtaa tatcgctagt tacctcatta gttgcacagg ctgtcatgat
    22021 taacagagac agtcctagca aactagtcaa ctttagtttc tttttcactc ccatttcctt
    22081 cctggtaaat ctttgataat tttaatacgt ggagtagatt tttctccatc tctgcgtatc
    22141 ccaaggtttc gactcctttt cgagcaatga caacaaagtc gacatcttct accagactcc
    22201 cttttgcatt ctggataata tgccgaatcc gtcgcttaat ttgatttcta gtgacggcat
    22261 tccccagttt tttgctaact gatagaccta ctcgaaaatg gtttttctgg ttttctaatt
    22321 ggtagaccac aaatttgcga ttagcaaaac ttgtcccctc cttgaaaatc gccttaaaat
    22381 ctttctctct ttttacacga aagtttttct tcaaaactca actccatcta ttaaattact
    22441 actattatac catatttttc aaaaaagcca atcatagcaa ggttttggac aatttcatca
    22501 gaaaaagaag ctggaaaaat aactttccca actccttgta ttgaatattt ttttagtttc
    22561 acttattttt tcaagcgttc aacgtcacgg gcaatgacta attcttcatc tgttggaata
    22621 accaagacac ggattttagc tgcctctgtt gagatgtctc ctgtaacgcc aaagacattc
    22681 ttttcatcat caacatcaca accaaaccac gaaatccctg agattacatc acgacggaaa
    22741 ttctctgcat tttcaccgac acctgctgtg aaaacaatgg catctgctcc atttagcact
    22801 gcaaggtact gaccgatatg tttttggata cggtcaacat acatttcata agccaagcta
    22861 gcctcgtgat tcccttctgc tacagctgct tctatatcgc gcatatcgct agaattagca
    22921 gaaactccca aaagacctga ttcacggtta agaacacgac tgatatcttc tggtgtgtta
    22981 aaatcctctg tatattgcat taaataagga atgatagctg gatcaatatc ccctgtacgc
    23041 gttcccatca taataccacc aagaggagtg aaccccatag aagtgtctac agatttgccg
    23101 gctttcacag ctgtaattga gcctccgtta ccaatatgac aggtaattaa cttcaagtct
    23161 tctaatggac gtcccaagag ttttgcagct tctcctgcta caaactggtg acttgtacca
    23221 tgagcaccgt atttacgaac cttgttttct gtgtaatatt ttgttggtag agggtagcga
    23281 taagctttct ctggcatact tgtgtggaag gaagtatcaa aaacaactac actggtaatg
    23341 tctggcaaca attccttgaa ggcacgaaca cctgctgcat tggccgggtt gtgtagagga
    23401 gccaacaaac tcaactcttc aactttttct aaaacatctc cctcaacaac tgttgattct
    23461 ttgaaatatt ctccaccagc aacaacacga tgtccaacac ctgtaatctc gtcataagcc
    23521 ttgataatat cgaaacgaat caagtcatcc aataaaattt taacagcttg tgtatgattt
    23581 tcgatatcca aaatttgttg ttcagaacgg ccgtcaaatt ttacagttga aattgaatct
    23641 ttcaaaccga tacgttcaat caaacctttg gctaacactt tttcctctgg cattaagtat
    23701 aattgccatt tcaaacttga acttcctgca ttgattgcaa ttgtttttgt cataacatga
    23761 tacccctttt taagcgtttt caactattat aacaaaatct attttatatt tcagtacctt
    23821 gagtccattt ttgaaaattt tctttaaatt tcattaaaac acttgcatct tgcaagctag
    23881 caagtggata aacaaaaggc tccactgcta tttcactttt cttctgtagg ataaaaatag
    23941 tcttagattg tttagcatta gcaaagagat tttcaggcag actaatcata gcaaccagac
    24001 tcgcctcttc tttcagccat tcttttaaca aatcactttg aggactggtc aacaaatcac
    24061 tcggagctag aaaaatagcg tatccgtctg acttgaggta cttaagccct tgttccatga
    24121 gcaagtgatg ggcgtaagta tgttcttggc tagaagcaac ttgatggcgc gacgcaacgg
    24181 catcatcagg ataatagcca acaggcaagt cactgatgac cacatcgctt tctttgagca
    24241 tttgtggacg aacggcatct ccttggacaa agccagcctg caaaccaatt acatctgcca
    24301 tgctagctgc cagatcaatc agcaaatcat ccacttccat tcccaaataa tccacctttt
    24361 tagtaagcga ggtcaagaaa gtagcgccca aaattcccat cccagaaccc atttcgagga
    24421 tagtaatttc ctcctcttta aacagctctt ccacaataaa caccaaaagt aaagcaatag
    24481 catccggtgt aaactggtga ttggcctgca agggttctgt ttgcccagcc ttcatcaaga
    24541 gaaactggta ggtcttgagc cattcttctt tgcgtagtgc taaacgctta agggtctgat
    24601 tgttgtcttt gacctgttct agctcagtct caccatccag atagatgctg ttttgctcca
    24661 ccaaggcgtc ataaaagttg gtcgccaaat cactttggat gacttggaca ttctctagta
    24721 aataggtata agcttgttca attttttcaa aatccatatt cctatcttat caaatttccc
    24781 ttcttttttc cattcttata gaaaaatcac tccctgacta ggcgagtgat ttttgggcta
    24841 ctattaaaaa gaattctgag tgatttcctt gaaacaggtc ttcagcgcta tagagaatct
    24901 gactatgcac agatgaaaaa ccatgctcaa ttagcttgtt ttccagttca gctaaatcaa
    24961 atccatgatg attagcttct gtcttggtaa aatcagcaat gatgagtttc ccatcttcct
    25021 tcaaatgttg atgaaacagt gagagagccg catccaaatc aggcatatga tgaagaaccc
    25081 gactaacagc aaggcaatca aactctttct ccaagggatt tttcggtaaa tcttgctcca
    25141 aaaactggat attcttgatt gcttgctgct ccactttcaa acgagcttgc tccaacattt
    25201 tctcagaaat gtctacaaga gtgactgact tagcctgctt ggctaggggt taggggcaag
    25261 gctaatagac ccgtgccacc accgaaatct aaaatttctt tgtctgatag aagatcaatc
    25321 tgtttctcgg ctgcttgaca taccaagttt gcgaggaaga tatttttagg gaaatcgaaa
    25381 gtttctgctt tgtggttaaa atcatgtttc atatctttaa tttagcacaa agtatagtaa
    25441 actgaaataa gatataaaca aatgaattgg agcttaacat ccatttccag caattttcta
    25501 gaaactacag tggactattc tggattcaac gcattatagt tgaattgact aggaattctc
    25561 tttcttttca ggcttctctt ctgatttttt cttctccact ttttcgcttg aatcggtcgc
    25621 tacctcttcc tttttatctc ttttctcttc tttgattttg actgaaggaa acagaaactc
    25681 atattggctc ttatccgtac gaaccctcgt cactaagcca gttttcttgt tttgatagct
    25741 tacctgacct agattaaaaa cctgttcccc actttcttgc tcaaccttat ctttggttcg
    25801 tttagccata gcaaaagcaa ccaatttttc tttattcaaa gcatagtctt gatagtgggc
    25861 gacttgtcgg ttcaaataaa attgcaacaa aagactaaag atggctgcta tggtgactgc
    25921 gtagaggaga acacctgcct taactttttt ctttttccac acgatagatg aactcccttt
    25981 ctaagccttt ttgaaactgg aaacgaaaat gaaccagttg attgtcctct gtaatctgag
    26041 ctgatttgag gccataaacc ataggctgat aacctcgacc acgagcattc gttttacgaa
    26101 aatcgtctga ctttgactta ccgatggcga tgtccttgcc atcttgcttc atgtataggc
    26161 gattgccttc tactttttcg aactgcgaac ggtctaattc tgcctcaagt tgatccacaa
    26221 acaagagcca ctccttttgc tcgctttgtt gctggtagcg aacttctgaa atgaggagct
    26281 gactcatagt ttgaaaaagg agtaatcccc cactgatgac aatgagggca agtagggatt
    26341 ctaacaaggt aaaagccttg accttatggc tctttgattg ccaacaactg ttctgaacca
    26401 tggtagacct ccaatccttt ttcactagaa aatacctgaa tctcaactcc gttgatgctt
    26461 acctgatttt gccccgtctg cagggccatc ttagctaccc tcaagacttc ttccttttgc
    26521 aagatttttg cttcctcttg cctatttttt tgaatttgtc ccaaaaggag ggtcgcaatg
    26581 ctggcaaaga tagctagagc gactactgct tccagtaaaa tcactgccct aattttttgt
    26641 ttccttaatg cgtttaattt ttccatttcc tagatataat tgatagcgaa tcgctccttt
    26701 actggtctga aattcaacct tagccaggga cgaattgccc ccagctcggt caaatgtaat
    26761 actttggcct gatggtgcct gaattccttt aggaactgtc aacttttgac tgccattgct
    26821 aagcgtctgc ccatctaaat ttagattagt cttttgctga ctggctacac tgcgtttttg
    26881 ggtttcccga tagagttctt caaactccat aaagaaaatc tgttcctcta ccgccgcaaa
    26941 agtggactga acagagccgg acaagcccaa ggcaaggata ctcacaagac ccaaaaccaa
    27001 gagactttcc agcatggtaa aggccttaat cattgacttt acgatttgct cccacatttt
    27061 tatcattgta ttctttataa gctttagcct gttcttccgt gatgcgtcca tcttctttta
    27121 acttgcttag gctagcatct tcattctttt ccaagctata aagttctgcc tggctttcca
    27181 ccaccttaac aacagctgct tttcctttgt cattgactgc ttctttttgc ttggtcagat
    27241 taggtacaaa gagcaagaaa agcacgctga taatcagcaa gaccaccaac atctccacca
    27301 atgtaaaagc tttaacctta gcttttttca agaatgtcat catttttttc attttaaaaa
    27361 tttacctcca tattttgata catgggcatg agcattgccg cataaagtaa aacgataatc
    27421 agtgccacaa agataaaaac cagtggctgc accaaattca tggtgcggtt gactcgggta
    27481 aaaaaggctt cccaagtttt ttcagcatag atttccaact cactacccag cttggactta
    27541 acttccccat actctatgat gagactcaat tccttcctaa agaaaggata ggttcctatc
    27601 gtctgagaaa attcacggcc atttttcagg gtttgagcca gatcttgacc gatttcttta
    27661 aagagctggg aaccttgttc ctgcatcatt tgaaaaatct gcgtcagctc cattccctgt
    27721 gaaatcatat tcccccattc acgtgcataa taggctgtca agtaggtctg cacaaagatt
    27781 ccaataaagg gaaggcgtgc taagatagaa aagacactca tcttagaact tcttttataa
    27841 aaagttagtg ctaaaagggc aagcacggaa acaagcccta ccatgcctag aaaaatttgt
    27901 ggcagattac cgataattcg ggtggcaata ttgctactat ccagttgtgg gagcaggtaa
    27961 ttccgtagcc ccagcataat taagagaaga aaacccagca aaatcaaagg ataggtcgct
    28021 acttcaatta actttttctt gaccttggcc agattgtcta gatattcttc tatctttccc
    28081 aaactcaggt gaagattgcc atgaacttca gctagagata actgagtgac aatggtactt
    28141 gaacatccca aactttccat catttctgag aatgatttcc cctgagacaa gcctgtacgc
    28201 atctgggtca cacactgctt gtccaacaag gaactcctat ctaaaaagga gatagtctcc
    28261 accagatgaa aaccgctaga aaagagattg ttaaataggg tgatgatatt tttttgctta
    28321 gctgtagcta attttttccg tctcagcctg aagacttgtg atatgtccat ctttaagaag
    28381 ctggtcaatt tgctcattcc acttggctgc ttggtgttct tgataatctc tgtttgcaaa
    28441 gtcaacgatt cctcctcccc cgattaatct ctggtagcag actccttgca gaacaactgc
    28501 caattcttct tcactcacac ccaactccag cagacgctca taaacacctc ggatactctt
    28561 ggcgtgaatg gttgaaaaga ctgtcgcacc tgtcaaacta gctctgacca ctgcacgcgc
    28621 cgtctcgatg tcacgaattt ctccgataat caagagatct ggtcgatgac gcaaggaaag
    28681 tttgattaga ttttcatagg ttagaccgat tgcttcgttc aactgcaact gaagcatgtc
    28741 gtcctgcttg atttcgacag gatcttcgat ggacataact tgctgtcctt taaagagtga
    28801 cttggacaat tcatgcatca aggtcgtctt accactccca accggaccag caaaaagata
    28861 gagtccccgt tgcctgtact gcttgcctaa ttcttcaata tcctgaaacc aaaaatgcaa
    28921 gtcctgctcc tcatcgtgca acaaacggat aaccaaactc tcatgccccc gataatcgcc
    28981 tacagtagat aaacgtagag acgccatctt ctggtcatag gcataatcac aggaacccag
    29041 ttgactacgt cttttttctc ccacattcat acccgccaca aacttaaagt gactgataac
    29101 ggctgcaaac ttttcaaaat catagctacc aattttacag cgctcgtctc ctaccctcat
    29161 atgaagctca taggcgtcta acttggggac aaaataaatg tcttgcgccc cttttttccg
    29221 agccgagcga atgatttctt gtgcaatttc ttgaaccata cttacctcct cacctatact
    29281 attcgcaaag cgttagaaaa ataaaaaaag caactccaaa aaagttactt ttctctattt
    29341 taatttcata cggcaagaac gattcctttc ctgactggtt tgtttttcat agcctgagcg
    29401 tagtttttcg tctgggataa cctgctcatc ctgcaaaaga gccaaagaat ggtattgttg
    29461 taaatactca tcacattcat cacaccagcg ttggtcttcc gggtcccaaa aaatggtcat
    29521 caagtcgctt ctacttttat aaagagggga ttcttgttcc aatctaaacc agcaagtttt
    29581 gtagtatttg agagcatcat aatactggtc aaattttcta cttgctacaa tatcttcttc
    29641 ccaaccttct aagaaccacc acggttcaaa atctccgtac atttctataa cacgatacat
    29701 attcattcat tcctttgtac cgtatattat aaccaattcc gccagacaag gaaagaaata
    29761 tgctcatttc ttaatttttt gataaaaaat ccagccatct gatggatgac tgaatttcta
    29821 tttacttatg tacaaaaata tttttactac ttaggcttct gagatatcgt tttcgataat
    29881 gaccttaatg gcatggtggt ctgctgcctt actgaagact tcgtaggctt tttcaatttc
    29941 actgagtttg aaatagtgag ttaccaattt ttctggttca atcttatgac tttcaagcgc
    30001 tttcaacaat tgtggagtcg tatttgtaga taccaaacca gttgttacat tgatgttgcg
    30061 aatccaaagt ttatctaaat cgaattcaac tggtttacca tgcacaccac agttggcaac
    30121 cgttccgtct acaccgataa tcttttgaca gaaatcaaat gttgcaggaa taccaacagc
    30181 ttcgatagcg acatccacac cacgaccatc tgtcaaatca taaatttctt taatggcttt
    30241 ttcagggtct gaagaattaa ccttatgagt cgcaccgaat gatagggcag tttccaagcg
    30301 gttatcgtct aggtctacca taatcaattt agctggtgaa tagaattggg ctgttaaaag
    30361 agcagccaat ccaactggac ctgaaccaat aatggctacg ctgcaaccag gttctacttt
    30421 cccttttaag acaccaattt catatccagt aggcagaatg tctgacagca taaccaaagc
    30481 ttcatctgac aagtcttctg gagtatggta aagagtatta tctgcatgag ggacacgtag
    30541 atattcagcc tgcataccat caatcaagtg accgaaaatc cagccccctt cgtcttcaca
    30601 gtgagcataa attccttttt tacagtagta gcatttacca caggcacaga cgcaagaaat
    30661 caagaccttg tcaccttttt tgaagttgga aactccttcc ccaacttctt caacaatccc
    30721 aatcccttcg tggccaagaa tggtaccact ttggcaagta ggaacatccc ctttgataat
    30781 atggaggtct gttccacaaa tagtggtttt tacaatacgc acaatagcgt ctgttggctt
    30841 gcgaataact ggtttgtcta catcaacaaa agaagcaagt cctggtttaa cataagtata
    30901 ggctttcata aagcactcct ccgttttttt atttgtaata tttatagttc aattgtaagc
    30961 ctttttcttt gtttcttcaa gctttttttg tgattttttt aactttttat ttaccagtaa
    31021 agtaccctgc ataaaaaagc agaagttagt ctctaacttc tgctttctct cttatgcttg
    31081 ataacgtttt acgccatcta ggtaggttgc taccaattcc aaatctttat ctaatacgat
    31141 gaagtcagcg tcgtagcctt cacggatttg gccacagacg tcatcgatgt gaacggattt
    31201 agctgggttg aagctggcca tcatgactgc ttcatgcgga ttcgcaattc cccattcgac
    31261 cacattcttc aaaccatctt tgagtttgag gatagaacct gccaagttac ctgtcgattt
    31321 gaggcgtgca gttccatttg caacgacaac tgggaattct cccaacatat aatctccgtc
    31381 ttccaatccc ccagctgtca tacagtctgt gataagagcg atgttttctg ttcctttttg
    31441 tttgataaga atttcgcaag cctttggatc tacgtggtga ccatcacaga tcaactctgc
    31501 ataggtatgt ggcaattggt acatggctcc aaccataccc aattcacggt gagtcaaccc
    31561 acgcattcca ttgtaggcat gcacccaaac actcgctcca gcatcgactg cttttttggc
    31621 ttcatcaaaa gtcgcgtttg aatgtccaag agcaaccgtc acaccttcgc ccgtaactgt
    31681 acgaacaaag tcttccaccc catcacgttc tggtgcaagg gcaattttat taagcaagcc
    31741 atttgccgct ttttgccaag aatgaaactc ctcaacaccc gggtctctca tataagttgg
    31801 attttgtgcc cccttaaaag tttctgtgaa atatggacct tcataataaa tcccacgaat
    31861 cttagcacct gttgcttctt tataatggtt tccaagattt tcagtgactg caagcaattg
    31921 ctcataagtg gctgttaaag ttgtgggtaa gaaactggta acaccggtac taagaagtcc
    31981 ttcactcata gtatgcaatg taccttcaat gttgttgtcc atcacatcta cacctgcata
    32041 tccatgaata tgagtatcca caagacctgg ggcaatgcta taacctgtat agtcaatcac
    32101 ctcagcccct tcaggaatct gctctacatg tttcccaaac ttgccgtcca caagttccaa
    32161 gtaaccacct cgacgaactc cgtgtgggta gaaaaactga tccgctttaa tatagttagg
    32221 cataatgtta acctccttaa aagattgatt ctacaattta ttatgtcaat tacattataa
    32281 acctttcttt tttatttgta aatggtatag acctattttt tggagatatt ttaaaagagc
    32341 tggatttctc caactcttct cttttttaat acaagttatt ttgatttaac cggcttgtct
    32401 tgagctgtct gcaaagctgt ggcaatcgta tctgcataca attttgctcc tgcttcgata
    32461 gtgctactct cactcccgaa atgaacctgg tctgttccag cccaaatttc tggatgctct
    32521 ttcgcaactt gattccaatc tgctatcgta atgtaaggtg tcttctctgc caattctctc
    32581 atataggcag cagccttctc aacgatggca taggtctctt ttgtcttatc tccctcataa
    32641 ggagtcacca aaatcatatg gtgtccctta ggaagatttt tcacgatact gtcccagtca
    32701 tccttgtaat tctcaggatt atttacccca gtcgcaatga ccaccgtctt aggtaaaaat
    32761 ttattctggc tattatttag catgatttca tttgcggtct tggttgttac gctgacctgc
    32821 gcgttaatct gtgctccagg aagagctgtc tgtagtgctg tatttgccct taaagccact
    32881 gagtcaccaa ttaacatagt gccatcagca attcccaaac tgtttgcatc tgcccgttct
    32941 gccatcacct tggtctggcc aatatttgtt gcagcttgct tcaagccatt gacagtcaag
    33001 tctgtctcaa acgctcccac ttgtggtgcc aacaaggtca ccgtgcagac aatgatggtc
    33061 aagattcctg tacctgctgc aagaattgcg tgaatataag gcaggggacg aagggtttgg
    33121 acaataggtg tgttcttgcc tgcaatccaa ggttccaata cataaaatga cagactggca
    33181 aagccataag aacaaatcag agtcagtaat acagcaagaa gatttgatgt caactgtgag
    33241 aaaatgatat agaaaggcca atggaaaaga taaaccgcat agctagtatc cgctaaaaag
    33301 ctgataatct tcggctcctg tatatgatgt gtcttttcat gtaagacacg cgccgccaga
    33361 atcatggcaa gagctgcaag actggcaagt aagaagccga taagataggc aaaaagataa
    33421 gtgaatttga caaagaaagt caaaagaact aggaaaccaa agcctcctcc aaaaactacc
    33481 aaagtctttc gtaaatccca gattttatcc aactgcttga cgagggaagt cgtctgacga
    33541 acgcctacaa tagttgctaa catacttccc aaaaagaatg gatagacatg agttaaactg
    33601 gagaaataaa cagaggaata agaggtcact agaaaactac caataaacat ggagaagaaa
    33661 ctgatcaaga aggcaacagc agataagaga aagaccatcc ccttcaactg accatttgat
    33721 ttagcttgtt tggataagaa ccaaactgcc aatccccaaa gaatatagta gtgaacctca
    33781 actgccaaac tccaattatg aacaaacaaa tgaggaatga actgagattc ataactccca
    33841 cctgttagga gttcatagaa gttggtcata aagcctaaga cgctcgcaat ctggccacca
    33901 attccagcaa catagtcttg gcgaaccaag aaagtaaaag gcatggtcac caagaccatc
    33961 aaaaccacag gtggcacaat ccgataaaag cgtcttctaa aaaatcctat caaatcaatc
    34021 tcatggtttt tagaaaattc ttcaatgagt agagctgtaa tcaggaaacc tgaaaatgtg
    34081 aaaaagacat ctaccccgaa aaatcctcca ggaaagatcg tctgaaagaa gtgatacaag
    34141 agtaccaaaa gtaaacctgt aatcctaacc aaggaaaacc atttaatgcg catacgagtt
    34201 tattcttcct ttcattgtac actttattct atcaaaaaaa gaagaaaaat cctaagagaa
    34261 aacttaggat ttttagctta tatcaattcc attttagaaa ttacgtcctg acttattata
    34321 gccatatttt tccacaaaat actcacggaa ttccaagaga ttgtcatcca tgatggcttg
    34381 tcgcacttgc ttcatcaagt taagcaagaa gtaaagattg tggtagctag tcaagcggat
    34441 accaaaggtt tcatcagcct tgagcaggtg acgaaggtaa gcgcgtgtat agttattaca
    34501 tgtgtagcaa tcacactcag gatccagtgg cgtaaagtcc tcagcaaact gggcattttt
    34561 cacaaccaaa cgtccttgac tggtcataca agtcccgtta cgagcaattc gagtcggtaa
    34621 gacacagtca aacatatcca ccccacgaat gaccccatcg atcaagctat ctggcgctcc
    34681 cacacccatc agataacgag gtttattttc aggcagcagt tgagttgtaa agtccaagac
    34741 cgcattcatc tcttcatggg tttctcccac tgccaaacca ccgatagagt agcctgagaa
    34801 atccatgctg acaagatcat gagctgattg gcggcgaagg tcttcaaatc ctgcaccttg
    34861 cacaattcca aacaaacctt ggtcatgtgg acgacggtga gccttcaaac cacgctcagc
    34921 ccaacggctg gtacgctcga tcgatttctt aacgtagtca taaggttgat aaaactgagg
    34981 acattcatca aaggacatca tgatgtctga acccagatta ttctgaatag agatggcttt
    35041 ttctggggat aggaacatct tagaaccatt tagatgattt ttaaaggtta ctccttcttc
    35101 tgtgatatta cggctatctg ctaaagaata aacctgaaaa ccaccactat ctgtcaagat
    35161 aggctggtcc caattcatga acttgtggag accaccagcg cgtgcaatga gttcatctcc
    35221 agggcgaagc cagagatgat aggtgtttga taggataatt cccgaaccca tctccttcaa
    35281 ttcttcaggt gactgagttt tgacagtggc ttgtgtccca actggcataa acataggtgt
    35341 cggaaaggta ccgtggggag tgatgatttc tcctagacga gctcctgtgt gtttttcttt
    35401 cttaatcaaa cgatatttga ttggtgaatc tgacattttt tacctccgaa gctgggaaag
    35461 acagtcccag ttcatacagt gtgcccaagg acacactgta tgattttatc aaaaaaagta
    35521 ggaactgtca cgaactatgt ataagaacta tggtataata agagaatgaa ctatccaaaa
    35581 attgatttaa aaacaattcg tcaggagtca aaacattttc aggctgacac cccccgtctc
    35641 tttctcctct atatccttcc cagtatgctg gtcatcttat ctggttttct taatccctta
    35701 tctcggatcc acgggactgt tttagaacag ccatttttca gcatacttgg ccaaatactt
    35761 caagcctacc tctttcctct actagtctcc tttatcggga ctattctttt gaccagttcc
    35821 gtctatgcaa cactgacact catgaaagat tctaaaacgg agccatctgt aaaaaacagc
    35881 ctagctctct ttgatgagga gcgcttttct caaacctttt tgaccctact tctcaaacgt
    35941 ttctatctct ttttatggag tatccctaat ttacttggga tttacctcct cttttatagt
    36001 agctttttag ccaaaaaatt cgtcacccta catcctgagt ttccaaatct agatctctca
    36061 tcagttgaaa ccgagcgttt cctcatggtc tttggactct actttcttgc aagtctcatc
    36121 ttgatcattg taggaaatat cctctatatt ccacagtatt atgcctattc acaggtggaa
    36181 tttcttctct gtcatagtct agacctagga caggtcccac caagacgaat cctaaaaact
    36241 agccgttcct ttatgaaggg ctacaagttc cagcactttg tcctagattt acagcttctt
    36301 ccttggtact tcctcaattg gattaccttt ggcattgcta gtttttcact cctaccttat
    36361 atccaatgca ctaaaattat gttttaccga gcagtattgg ctcgaaaacg tccaaaagct
    36421 tgaaggtttt cccctcaagc ttttttctat caatgaattt ctccgcctac caacttgagt
    36481 tcctgatctc catgttctat catagcgccg attgctgctt ctatccctcg ccgaatatcc
    36541 actaaactca tagttggagt agtcggtctg ttcaccacct gttccatcat ataaggaata
    36601 tgcataaaac ctgccttaac atatggaaat ttcttttcta ccaaatagag agcctgatac
    36661 atcaaatggc tgcagacaaa agtccctgcc gtattggaaa cagaggccgg taatccttct
    36721 tttttatagc ttgaaccatc gctttaatcg gcaaactact aaagtaggcc gaagcaccat
    36781 ctgggcgaat gggacggtca atcggttgat tatcttcgtt atcagaagta cgtgcatcgt
    36841 cttgattaat ggccactcgt tcaggtgtca aactagttct tccaccagct tgcccaatac
    36901 aaaggacaaa gtcaggttga taacgattca tctcttcttc caatacttga gcagatttgt
    36961 gaaaaactgt cggcacctct agccaacgga cctcagcacc atggatttca gctggtaaac
    37021 ctttaatggc ctcaaaaggg ctcaaaacct gtcactaata ctttcatttc taactcctca
    37081 aaaccaattc aatgaaactt ttttcttaaa aacaagtata acatatttcc cttattctgg
    37141 attaaaaagg attagcgaga ttagaatcga ccttcaagtg tatttaaaga gtaaaatact
    37201 aatcaaggcc aaaatagctg atccaccttg tttcaaaata atttttttat ccgctgttaa
    37261 agagccgtaa gtcgcagcac caatcacaaa taagacaaaa atagtcacaa tttctaaatt
    37321 ctgtgagaaa taaatgacat acaagagaaa gactcctagc agagccttat aaattccttg
    37381 atttttgaac aatgaactta ctgacggatg agccaattct tccttttcca tattaaatac
    37441 acgactagtc gcatctgatt gcgtggcaat actttccaaa taaaaaatgt aaaaatgctc
    37501 caaagcaacg atcgttgtta aaataattga catctatttc tccttaaatt tctatatttt
    37561 caatacccaa aaccaattta tttaataaac ggctgagttc tgttctctca aactctgtta
    37621 agatactttc tatctcttcc ttaaccctga tatgttgcag agggggattc accactaact
    37681 gctccttggc atacttcgca gcctctacca acacttcccg ctgattttca ggattacgat
    37741 gacgctccac caaaccttcc gtttccaaaa ttttgaaatg ccgtgtcaaa gcagcctgat
    37801 caattttcaa acgctcctga accgccattt ggttacaagg agaatgctcc agcaaaaaca
    37861 gtaaaatctg ataccgtgtc aaactaatcc caagcctttt ttcaaacaat tgcgtactcg
    37921 cttgctcaga caagcggagt tgatacagta aatcttgaat ctctagcatt tcttcccctc
    37981 tctttttatt gatttatcaa tatttgacta attaattgtg atataaagta aaatagattg
    38041 caagaatgtt gcttaactca ttacaagatg ataactttcc attttccatt caaattgata
    38101 ctcaatgaaa atcaaaaagc aaactaggaa gctagtcgca ggttgctcaa agcacagctt
    38161 tgaggtctca gataaaactg acgtggtttg aagagatttt cgaagagtat gacaaagaaa
    38221 aaacgaacga gaatcgcttt ccatcacgaa agctagatct cattcgtcaa aatgatgtta
    38281 tcaagtcggg tttatccatt cattgaaacg tgaacatgat catagtgatt ttctgtcaca
    38341 ctaccgcggt ctggcattgg gttccaagtg ttagctggcc catatttgct atcgaatgga
    38401 gcatagaaac gttgtttcca gatgatgtaa ctaatgccac ggctggccat attttgaata
    38461 gcatattccg caatcttatc ccctaattct gaacgttctg gtaccataaa gtcgatagcc
    38521 aaaccttttc cgtgatctcc actgtctcct ggacgataac cactaaagga tgtaatgcca
    38581 aacaagttag caatttcttc tttaaaggca gctgtttgtg gttgaagacc tgcattttca
    38641 gattttgcta ctgcaagtcc agcataatcg ggcgcagctg gagcctcgta agttgttgaa
    38701 attactttcg tttcttctga ttgataagta gaaactgctg ctgtagctcc atttgatgat
    38761 gctacttctt ttgcttctga acttgttgta gttgcttcca ctgctaatgc agcttgaggg
    38821 cttgcttgtg tttcctgctt ctcagctgga gtcgtttcct gaggagttgc ttcttcaact
    38881 gggcgagttg tttcggccgt ttgctcctct gggacagaag tgcccgtaga tggtgccact
    38941 tcttcagaag caatcactgt ctttgtaact tctgaacttg atgctacttc ttttggagct
    39001 tctgcaattg gttgagaaag gtctgcaacc tgaacagttt gatcatcaac ggtcacttga
    39061 ttagtggtca aatctgctgt cgcagttgtc acttcttcac tagagtctgc ttgaggtgtt
    39121 tggatttcaa cttctgttac ttcttctgct tcattgacag tcgttgtcaa aacagtttct
    39181 gggaaaatca agtccatatt agtgattttg ttcagattcg caagcactgt gacatctaca
    39241 cccaaggctt ctgcaatggt gctcaaagta tcaccatact gtacggtata acttgttttg
    39301 ttgtccgttt tagtcaaatc gttttggatt tgctcaacac tacgtgcagt ccaaagaact
    39361 tcttctgctt gagttgccaa tactggggca aatgacaagg ctactgttga cgctaataac
    39421 attcttttct tcattctttc aaatcctttc aaatgagtac ctgtctatga taacacaaaa
    39481 aatgtagaaa aaaagccctc tcgggcctat ttaacagaac tgtctattcc ttgtaacaaa
    39541 ctaggttact tgaaatagtt tgttaggtct ctgtcacaaa actgacacaa tctttttatc
    39601 gttcagcctc aaaaatacgg gaaatatctg ctaacgcttg aatcctgtga ttcccttcat
    39661 aagtagactc taaaaagttg atactttgaa tcccactatt ctgggcaaat tccacatcca
    39721 gagtccgatc ccctatataa taagtattat cagaattcaa ctgatactta tctagcagat
    39781 aggtagccgc ttctggactt ggcttccgca caaagccact ctgactggtt aaaatctctg
    39841 taaaatagga ttccaccccc aagtccttga gaatggtaaa agcgttgttc cccttatgag
    39901 tatatataaa ctgctgaatt cctgattcgt ctgcccaagc tagcacctca cgcgcacctg
    39961 gcatcaaaac tacctgagca ttcttctcag ccagactctg ggcacgcacc tgatttagca
    40021 cctcaacatc cagatttcta tcttctgcca cccgcacaag caaatcttgc accgaatact
    40081 tgaagataaa ctctctcacc ttctccttat cataaggaat agaaaactga gcaaaagtct
    40141 cctcaatccc tgataaaatc gcttcgtaag agtccaataa agtcccgtct aaatcccaaa
    40201 taaaagctgt tttttgcatt tcctatcctt tcaagatcat atagcgctgg taacgatagc
    40261 gtagaaataa ccagcgaaaa ccattatcca agagagaccc tgcccaaata ccaggtaagc
    40321 cccaaccaag cacaatcccc atcagatatc ctgttccaat gcggatacac cacatgccta
    40381 tacttgtcgc ataaaaaggg aggcgtgcat ttcctaatcc ctgccagact gccgtataga
    40441 tgactgttcc tgtcgtcata ggggttccaa gtagtgaaaa cagtgtcact agaacactag
    40501 cctccaccgc tagagaatca gtcgtataga gatgagttaa tggtacaccc aagacatata
    40561 tactaaagga taggggcaac atgaggaaca gagaaagcca aaaggtttgt ttactcaaac
    40621 tagcaactct tttccaatca tcctctccaa ctgctcgggc caacagcatg accgttgccg
    40681 tagcgacgcc aaaggcaggc atatagttaa actgggtcaa gacttctccg attgcattcc
    40741 cagcaactgc ctccgtccca aaagaaacga ccaaggcaat gatcactaca tctccagccc
    40801 tcatcataag tcgctctcca gctgctggta aagccaaggt caacagttcc ttatctaaac
    40861 caaaagttgg cttcccataa ggcagtttta attgtgacca caaaatcaca agaccaacca
    40921 aacgagacag aattgtcccc caagcaacac cagctatccc catatccaga acaaaaatag
    40981 ctagacttga aaaaagaata ttcaaggcat tggataaaaa actaacatag agaggcagac
    41041 gtggattatg cgttgcacga atcaaggctc ccagactagt cattaaacct aagagaacaa
    41101 tcgatccgcc taccaaagat agatacagtc caccactctc agctacatcc ctctccgtcc
    41161 ccaaaagtcc tatcatctct ttcccagcga agatggacaa aaatcctaaa aggaaactta
    41221 atagtaaggt aatcttcaac gcctcagtca catgataggc caacttcgac tggtctttct
    41281 gccctatgct ttttgaaata acactggaaa tagcagctcc cagagcgatg aaaatcgcct
    41341 gataaatggt gataatatta ccagctaccg aaacccctga aatagctatc aatcctaaat
    41401 gagcaaccaa ataactgtcc accattccca tgagcatctg caaaaagttt tcacccatag
    41461 ctggcaatgc aatattaaga atgtctttat ttttcttaaa caatctctcc tcctgatgaa
    41521 aagaaactca gttggtttcc caaccgagtt tactccctct atcttaaagt cctaaataag
    41581 cctcaaccgc tacttgcatg tcagcagctg ccactgttgt cttgtgacga acaggagctg
    41641 tctcaagccc atcaactgct ggtggcactg caacgcctga gatttcatgt aattgagcca
    41701 aagcttcaaa gtctgttaaa cctgcttttc cagttacagc ttctactgca actactggga
    41761 acttgtaggg actagctgtt gaagcaatca ctgtcttagt cgcatcatca gtaaccgctt
    41821 ggtattttct ataaactgct gaggcaaccg ccgtatgtgg atcctcaata taagaatcta
    41881 actcataaac acgcttgatt tctgccgctg tttcttcctc agtcgcatat tcagctgcaa
    41941 agagctccag aatctctaca tcaaaatcag tcagttcata ttgtccttgt gtattcaagg
    42001 cattcatgag ttcagccgtc ttaaccgcat cattccccaa aagatggaaa atcaaacgct
    42061 ccaagtttga agataccaag atatccatag atgggctggt tgttacctta aactcacgtt
    42121 tcttgtcgta aacacgtgtc ttgaagaagt ctgtcaaaac attgttatca tttgaagcac
    42181 agatcaattt accaactggg agaccgattt gtttggcata aaaggcagcc aagatatttc
    42241 caaagtttcc tgttggtact gtgaagttaa tcttatcacc agccacgatc tcaccagtct
    42301 tgaccaactg agcataggca taaacataat aaacaatctg tggcaccaaa cgaccgatat
    42361 tcatagagtt agcagatgaa aattgcaact tgttggccgc taatctttca cgaagagcca
    42421 cgtcgttaaa catgtgcttc acgttggttt gcgcatcgtc aaagttacca tctatagcga
    42481 taacatgagt attgtcacca ttctgagtgg tcatttgcaa ctcttgtacc ttgctgacac
    42541 caccctttgg ataaaagacg ataatctcag taccaggcac atccgcaaac cccgccatag
    42601 cagctttccc cgtgtcacca gatgtcgctg tcaagataac aatcttgttc tccaaaccat
    42661 gtttcttagc agcagtcgtc ataaagtatg gcaaaataga caaggccata tccttaaagg
    42721 caatcgttga accatggaaa agttccaaat tgtattgccc atctaatttc actaatggtg
    42781 caatagctgg agtatcaaat ttgctatcgt aggcattgtt gatacagtag tccaactcct
    42841 cagctgtaaa gtcatctaaa aatgctgaca aaactagctt agcaacttcc tgataagaag
    42901 catctttcaa tttgtcaaag tccaaatcta cctttggata agtaaccggt gtaaacaaac
    42961 cgccgtccgt cgccaaacct tgcaaaattg cttggctggc agttactgta ttgttggcat
    43021 cacgcgttga ttgataaact aatgtcatta ctctctatcc tttatctata gtctcgtcca
    43081 ttatatcatg atttttcaga aaattctccc tttataagag aaattatagg ctcaattctt
    43141 ctctcaaagg ctgtaaatag gtcatttctt ccttatttct caccactagt ccctcctcag
    43201 ctagtaggag taagtctttg gaaaattcaa taatcgtagt ttcttcctca tctgtaagat
    43261 ttttcttaga aaattgtctc cttaaaaact tgtaatcata accaaatact ttaaagaaag
    43321 gtgctgtttc taagtaagct tctaacttgt ctaaattaac caataatccc aagtgaaaag
    43381 ctgcagaagc aaaagtccta tcaagtggct gtgtacacac actacgaaac tcaactgttc
    43441 ctcgagtcgt taagtcttgg tactggtaac tacgatgagt ttcaaaatcc ttctcttggg
    43501 ggtaaataat aacctcatcc ccattcagag caaatgcttg gatttcaggc gtagccaaat
    43561 agtccccagc ctgaatagga taaaaataat aggtctgccc atcacgttcc gcagtaaaaa
    43621 tcgcagaatg atttagatag tcaaaaaaat cagcttcatc attaaggagt ctagcattga
    43681 ccccaacatt ctctggatag ataccatgca tagattcttc ccagaaaata tcccttgaaa
    43741 ttttcgtatc ccaatccgca cccgaaaact cagagtttgc aaataaataa gccttagccg
    43801 cttcaatttg agtaaaagca ttaatcaccc gtaagtagtt agtttttgaa atatccagct
    43861 gaacctggct cccacagata aaagtaccat attcagggaa atgatgtaaa tctgatttaa
    43921 taatatttct actcaaattc aaataatcca tcaacatctg atagcgtgga taagccactg
    43981 gacaattctc atttttatcc cagttgggat ggataccaca gccaacaata gcatgatttg
    44041 attcagctaa ctttctctga attacattca tataattatt gaaacgattt tcgacctctt
    44101 gaatcgtttc agccttacca aatgcaaact caatcgttgt ataggaaact tcaaacaaaa
    44161 tagcatcctg acttatcgga tctactaact gaatcagatt gccaaaatca tctacctttg
    44221 ctacggtgag atccaaagta gaaactaaat aatgaaatag atcctttata acttcaacat
    44281 ctgtagcatc cccttctaaa cttgcaacag gatactccaa ctcaattccg acaaacaatt
    44341 caggattttc tttaatattt ttcaaatacc gatgttttaa taaatcgata gaacaagaca
    44401 tactacccta cactttctca atctaaataa aatttgaata actataatta taccaaaaat
    44461 aatataaaaa aggaacgaag aataacaacg ttcctaacac tatactatac cggcggccgg
    44521 ggtcgaaccg gcacgtcctt gcggacactg gattttgagt ccagcgcgtc tgccaattcc
    44581 gccacgccgg caaatagtaa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
    44641 agtcccttgc cttaccgctt ggctataccc caataatata aaataggcga gtgatgggga
    44701 tcgaacccac gcatgccaga gccacaatct ggtgtgttaa ccacttcacc acacccgcca
    44761 taattctatt aacacgggca gtaggaattg aacccacact gaaggttttg gagaccttag
    44821 ttctaccttt aaactatgcc cgtaaaatgg aaggggaggg attcgaaccc ccgaacccga
    44881 aggagcggat ttacagtccg ccgcgtttag cctcttcgct acccttccaa aatatataaa
    44941 tggcgcgaga cggaatcgaa ccgccgacac atggagcttc aatccattgc tctaccaact
    45001 gagctaccga gccttattgc gggagcagga tttgaaccta cgaccttcgg gttatgagcc
    45061 cgacgagcta ccgtgctgct ccatcccgcg ttaatataaa aggaggatgt gggattcgaa
    45121 cccacgcacg cttttacacg cctgacggtt ttcaagaccg ttcccttcag ccggacttgg
    45181 gtaatcctcc aaaatagttt atacgggaac aagcccgtgt cctcttagtg aacagataat
    45241 attccaatcc cctagtaatg gactaaacac tatgggcacg agtggactcg aaccaccgac
    45301 ctcacgctta tcaggcgtgc gctctaacca cctgagctac gcgcccaagt taaaaacttg
    45361 gtacaaagaa caaagttcaa agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa
    45421 ggctgtagtt ttaccactaa actacacccg ctaaaatggg agttaacggg atcgaaccgc
    45481 tgaccctctg cttgtaaggc agatgctctc ccagctgagc taaactccct a
//
LOCUS       DAVKAO010000009        42851 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKAO010000009 DAVKAO010000000
VERSION     DAVKAO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.009"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..381
                     /locus_tag="VWX16_000528"
     CDS             <1..381
                     /locus_tag="VWX16_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9157926.1"
                     /translation="EREIVTPTYREALITREKSFPTGLDMEFLGKDLPNVAIPHTDIV
                     HNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNILAQLMDFFTGN
                     GHLEDLSKISEPEKLYAYIAEATA"
     gene            419..724
                     /locus_tag="VWX16_000529"
     CDS             419..724
                     /locus_tag="VWX16_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9157927.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            772..2247
                     /locus_tag="VWX16_000530"
     CDS             772..2247
                     /locus_tag="VWX16_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9157928.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2270..2347
                     /locus_tag="VWX16_000531"
                     /pseudo
     CDS             2270..2347
                     /locus_tag="VWX16_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2597..9298
                     /locus_tag="VWX16_000532"
     CDS             2597..9298
                     /locus_tag="VWX16_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9157929.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDTKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSASSQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9546..10402)
                     /locus_tag="VWX16_000533"
                     /pseudo
     CDS             complement(9546..10402)
                     /locus_tag="VWX16_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10613..10972)
                     /locus_tag="VWX16_000534"
     CDS             complement(10613..10972)
                     /locus_tag="VWX16_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9157930.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLTLSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYVEMIVELFLMK"
     gene            11064..12020
                     /locus_tag="VWX16_000535"
                     /pseudo
     CDS             11064..12020
                     /locus_tag="VWX16_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12004..12561
                     /locus_tag="VWX16_000536"
     CDS             12004..12561
                     /locus_tag="VWX16_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9157931.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12554..12808
                     /locus_tag="VWX16_000537"
     CDS             12554..12808
                     /locus_tag="VWX16_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157932.1"
                     /translation="MDKQYLREKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12820..14874
                     /locus_tag="VWX16_000538"
     CDS             12820..14874
                     /locus_tag="VWX16_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9157933.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15053..15913
                     /locus_tag="VWX16_000539"
     CDS             15053..15913
                     /locus_tag="VWX16_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9157934.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            16132..16842
                     /gene="ccdA2"
                     /locus_tag="VWX16_000540"
     CDS             16132..16842
                     /gene="ccdA2"
                     /locus_tag="VWX16_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9157935.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16862..17428
                     /locus_tag="VWX16_000541"
     CDS             16862..17428
                     /locus_tag="VWX16_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9157936.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17439..18551
                     /gene="msrB"
                     /locus_tag="VWX16_000542"
     CDS             17439..18551
                     /gene="msrB"
                     /locus_tag="VWX16_000542"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9157937.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKTVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18819..19556
                     /locus_tag="VWX16_000543"
     CDS             18819..19556
                     /locus_tag="VWX16_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9157938.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYSQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19553..21244
                     /locus_tag="VWX16_000544"
     CDS             19553..21244
                     /locus_tag="VWX16_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9157939.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRVETS"
     gene            21360..21923
                     /locus_tag="VWX16_000545"
     CDS             21360..21923
                     /locus_tag="VWX16_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9157940.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            21945..27380
                     /locus_tag="VWX16_000546"
     CDS             21945..27380
                     /locus_tag="VWX16_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9157941.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPSPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQQENPKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETVNQPVEQPKVETPAVEKQTEPTEEPKVEVTSIPQTTRYEEDLTKE
                     HGTREVVKEGKNGSRTVTTPYILNATDGTTTEGTSTTDEAEMEKEVVRVGTKPKEKLA
                     PVLSLTSVTDNAMLRSARLTYHLENTDSVDVKKIHAEIKNGDKVVKTIDLSKERLSDA
                     VDGLELYKDYKIVTSMTYDRGNGEETSTLEETPLRLDLKKVELKNIGSTNLVKVNEDG
                     TEVASDFLTSKPVDVQNYYLKVTSRDNKVFRLTVEKIEEVTEEGQPLYKVTAKAPNLI
                     QHTDATKMQDEYVYYIEKTRATDGDIYYNFNDLVNAMNKNKTGTFKLGADLNATGVPT
                     PAKSYVTGDFRGTLTSVDGEHYTIHNTSRPLFNNIIGGTIKDINLGNVNIHMPWANNV
                     ASLANIIKGGTTIENVKVTGNVLGKDWVSGFIDKIDSGGTLRNVAFIGNVTSVGTGGS
                     FLTGIVGENWKGLVEQAYVDANIRGKKAKAAGIAYWSQNGGDNYAVGRYGAIKKSVVK
                     GSIDVEKPIEVGGAVGSLNYLGYIEDTVAMMKVKNGEIFYGSHDIDTDPYYTGERVNR
                     NFIVDGVSEGKSSYKYSKQQNRIKSVSQEEADKKIKELAITADKYAITEPIVNKLNAL
                     TTRDNEYRTTQDYKADRELAYRNIEKLQPFYNKEWIVDQGNKVPSNSKLLTTEVLSVT
                     GMKDGQFVTDLSEIDKIMIHYADGTKEEMNVTAVADSKVKQVREYDVTDLGVVYTPNM
                     VDKNRDQLIADVKAKLSSVELISPEVRALMDKRGKAEENTEGRQNGYIRDLFLEESFA
                     EVKAGLGKLVKALVENEDYQLNSDEAAMRALIKKVEDNKAKIMMELAYLNQYYSFKYA
                     ELSIKDIMMFKPDFYGKNVNVLDFLIKIGSSERNVKGDRTLEAYRETIGGTIGINELN
                     GFLHYNMKLFTNHIDINDWFKKAIEKNAYVVEQPSTNPAFANKKYRLYEGINNGQHGR
                     MILPLLNLKNAHLFMISTYNTISFSSFEKYGKDTDEKREKFKSEINKRAKEQVNYLDF
                     WSRLATDNVRDKLLKSQNVVPTPVWDNHNSPNGWASRHGHIDGKPDYAPIREFFGRIN
                     KYHGYKYGYGAYAYIFAAPQPMDAVYFVMTDLISDFGTSAFTHETTHINDRMAYYGGH
                     WHREGTDLEAFAQGMLQTPSVSNPNGEYGALGLNMAYERQNDGNQWYNPNPNKLKSRA
                     EIDHYMKNYNEALMMLDYLEAESVLPKLKGNNDRWFKKMDKQMRKDGQPHQFDKIRDL
                     NNEEKKIQLASIEDLVDNNFMTKHGAPGNGTYNPSDFSSAYVNMNMMTGVYGGNSSDG
                     APGAASFKHNTFRMWGYFGYENGFIGYASNKYKAEANKAGQTLSDKYIINKVSGGTFN
                     TLEAWKKEWFKQIKTKAQKGFTAIEIDGKTIDSYEKLKDLFDKAVEEDLKGTGTDKTV
                     KLKEKVYKQLLKNTDGFSGDLFTAPQA"
     gene            27449..29170
                     /gene="pabB"
                     /locus_tag="VWX16_000547"
     CDS             27449..29170
                     /gene="pabB"
                     /locus_tag="VWX16_000547"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9157942.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVAEVADLLAQVESYQ
                     EQDYYVVGYVSYEAAPAFEEKLAVHKVPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAENYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLGQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            29171..29788
                     /locus_tag="VWX16_000548"
     CDS             29171..29788
                     /locus_tag="VWX16_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9157943.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGATLTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            29838..30828
                     /locus_tag="VWX16_000549"
                     /pseudo
     CDS             29838..30828
                     /locus_tag="VWX16_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            30963..31922
                     /locus_tag="VWX16_000550"
     CDS             30963..31922
                     /locus_tag="VWX16_000550"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9157944.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            32019..32858
                     /locus_tag="VWX16_000551"
     CDS             32019..32858
                     /locus_tag="VWX16_000551"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9157945.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(32906..33076)
                     /locus_tag="VWX16_000552"
     CDS             complement(32906..33076)
                     /locus_tag="VWX16_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9157946.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            33197..34081
                     /gene="miaA"
                     /locus_tag="VWX16_000553"
     CDS             33197..34081
                     /gene="miaA"
                     /locus_tag="VWX16_000553"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9157947.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            34074..35312
                     /gene="hflX"
                     /locus_tag="VWX16_000554"
     CDS             34074..35312
                     /gene="hflX"
                     /locus_tag="VWX16_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9157948.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            35305..35928
                     /locus_tag="VWX16_000555"
     CDS             35305..35928
                     /locus_tag="VWX16_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9157949.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            35943..36872
                     /locus_tag="VWX16_000556"
     CDS             35943..36872
                     /locus_tag="VWX16_000556"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9157950.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            36893..37648
                     /locus_tag="VWX16_000557"
     CDS             36893..37648
                     /locus_tag="VWX16_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9157951.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(37702..38610)
                     /locus_tag="VWX16_000558"
     CDS             complement(37702..38610)
                     /locus_tag="VWX16_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9157952.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            38674..38943
                     /locus_tag="VWX16_000559"
     CDS             38674..38943
                     /locus_tag="VWX16_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9157953.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            38943..39323
                     /locus_tag="VWX16_000560"
     CDS             38943..39323
                     /locus_tag="VWX16_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9157954.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNKEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            39412..39639
                     /locus_tag="VWX16_000561"
     CDS             39412..39639
                     /locus_tag="VWX16_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157955.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(39679..40404)
                     /locus_tag="VWX16_000562"
     CDS             complement(39679..40404)
                     /locus_tag="VWX16_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU9157956.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLKGDILVDSCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTIC
                     KPARLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            40566..42407
                     /gene="typA"
                     /locus_tag="VWX16_000563"
     CDS             40566..42407
                     /gene="typA"
                     /locus_tag="VWX16_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9157957.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            42427..42681
                     /locus_tag="VWX16_000564"
     CDS             42427..42681
                     /locus_tag="VWX16_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9157958.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT        13061 a         7999 c         9584 g        12207 t
ORIGIN      
        1 gaacgagaaa ttgtcactcc aacttatcgt gaagccttga tcacgcgtga aaagtcattt
       61 ccaactggtt tagatatgga atttctagga aaggacttgc caaatgtagc gattcctcat
      121 acagatattg ttcataatct agctgagaaa gtggtggttg ttcgattaga aaaaccagta
      181 acttttcaca atatgatagc tcctgataag gaagtagaag tatccttgct cttctttatc
      241 atcaataact caagttcaag tcaaacaaat attctggctc agttgatgga ctttttcaca
      301 ggaaatggac atcttgaaga cctatcaaaa atttccgaac cagaaaaact ttatgcttac
      361 attgctgaag caaccgctta atcttgtcta ttaaaaaata aaatcggagg aaatccaaat
      421 gattaaaatt cttgctgcct gcggtgcagg tgttaactca agtcaccaaa ttaaaagtgc
      481 tctagaagaa gaactttcaa accgtggtta tgatgttcac tgtgatgcag tcatggtgaa
      541 agatgtaaac gaagacctta tgaaaggtta cgatatcttt acaccaatcg ctgcaacaga
      601 ccttggtttt gaaccaggta tcccagttat cgaagctgga ccaatcttat tccgtatccc
      661 agcaatgagc gctccagtat ttgacaatat tgaagcagct attaaagaac acggattaag
      721 ctaattctaa tttgttaaca ttcatataac ataaaaaagg gaggaactgt tatggatgca
      781 atctttgacc taatcggaaa ggttttcaat cccatcttag aaatgggtgg acctgtcatc
      841 atgttaatca ttttgacagt attggcttta ctttttggag tgaaattctc caaagcgctt
      901 gaaggtggta tcaaacttgc catcgctctt acaggtatcg gtgctatcat cggtatgcta
      961 aatggtgctt tctcagcatc acttgcaaaa ttcgttgaaa acactggtat acaattgagt
     1021 attaccgacg ttggttgggc accacttgca acaatcactt ggggttctgc ttggacactt
     1081 tacttcttgc tcatcatgtt gattgtcaac gtagtgatgc ttgctatgaa gaaaaccgat
     1141 acacttgatg tcgatatctt tgatatctgg cacttgtcta tcacaggtct cttgattaaa
     1201 tggtatgctg ataacaatgg tgtgagtcaa ggggtttcac tcttgattgc tacagcagct
     1261 atcgttcttg tcggtgtgtt gaaaattatc aactctgact tgatgaaacc tacatttgat
     1321 gaccttctta acgccccaag ttcatcacca atgacatcaa ctcacatgaa ctacatgatg
     1381 aacccagtta tcatggtttt ggataagatt tttgaaaaat tcttcccagg ccttgataaa
     1441 tatgactttg atgctgctaa attgaacaag aaaatcggtt tctggggatc taaattcttc
     1501 atcggtttca tccttggtat cgttatcggt attatgggaa ctccacatcc aattgcaggt
     1561 gttgcagatg cagataaatg gcgtcttgtt atcaaaggat ggttgtctct tggtttgact
     1621 gccggtgtat ctttggaact cttctcactt atcggttcat ggttcatcgc agccgtagaa
     1681 ccactatcac aaggtattac aaacgttgct actaaacgtc ttcaaggacg taaattcaat
     1741 atcggtcttg actggccatt catcgctggt cgtgctgaaa tctgggcttg tgccaacgta
     1801 cttgcaccaa tcatgttgat tgaagcagtg cttctttcaa aagttggaaa tggtatcttg
     1861 ccacttgcag gtatcatcgc tatgggtgtt actccagctc tcttggttgt aactcgtggt
     1921 aaattgctcc gtatgattat cttcggaaca ctcttgttgc cactcttcct tctttcaggt
     1981 acccttattg caccatttgc aacagaactt gctaaaggtg taggtgcctt cccagaaggt
     2041 gtgagccaaa ctcaattgat tactcactct actcttgaag gaccaatcga aaaacttctt
     2101 ggttggacaa ttggtaacac tacaactggt gatatcaaag caatccttgg tgcagtagcc
     2161 ttccttgtat tctatatcgg tatctttgct tggtacagaa aacaaatgat caaacgtaac
     2221 gaagagtacg cagcaaaagc aaaataatgc gctcctataa aatataaact caatgaaaat
     2281 caaagagcaa actaggaagc tagccgcagg ttgctcaaaa cacagttttg aggttgtaga
     2341 taagactgac gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt
     2401 cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagta atcatatcta
     2461 cggcaaggcg acactgacga agtttgaatt ggattttcgc agagtgtaaa ttgttgaaac
     2521 taggttgtca taccatgtat accacttggg ctttggtagt agctaactgc actaaatata
     2581 atataaggag ggttttatgg ggaaaggcca ttggaatcgg aaaagagttt atagcattcg
     2641 taagtttgct gtgggagctt gctcagtaat gattgggact tgtgcagttc tattaggagg
     2701 aaatatagct ggagaatctg tagtttatgc ggatgaaaca cttattactc atactgctga
     2761 gaaacctaaa gaggaaaaaa tgatagtaga agaaaaggct gataaagctt tggaaactaa
     2821 aaatgtagtt gaaaggacag aacaaagtga acctagttca actgaggcta ttgcatctga
     2881 gaagaaagaa gatgaagccg taactccaaa agaggaaaaa gtgtctgcta aaccggaaga
     2941 aaaagctcca aggatagaat cacaagcttc aagtcaagaa aaaccgctca aggaagatac
     3001 taaagctgta acaaatgaag aagtgaatca aatgattgaa gacaggaaag tggattttaa
     3061 tcaaaattgg tactttaaac tcaatgcaaa ttctaaggaa gccattaaac ctgatgcaga
     3121 cgtatctacg tggaaaaaat tagatttacc gtatgactgg agtatcttta acgatttcga
     3181 tcatgaatct cctgcacaaa atgaaggtgg acagctcaac ggtggggaag cttggtatcg
     3241 caagactttc aaactagatg aaaaagacct caagaaaaat gttcgcctta cttttgatgg
     3301 cgtctacatg gattctcaag tttatgtcaa tggtcagtta gtggggcatt atccaaatgg
     3361 ttataaccag ttctcatacg atatcaccaa ataccttcac aaagatggtc gtgagaatgt
     3421 gattgctgtc catgcagtca acaaacagcc aagtagccgt tggtattcag gaagtggtat
     3481 ctatcgtgat gtgactttac aagtgacaga taaggtgcat gttgagaaaa atgggacaac
     3541 tattttaaca ccaaaacttg aagaacaaca acatggcaag gttgaaactc atgtgaccag
     3601 caaaatcgtc aatacggacg acaaagacca tgaacttgta gccgaatatc aaatcgttga
     3661 acgaggtggt catgctgtaa caggcttagt tcgtacagcg agtcgtacct taaaagcaca
     3721 tgaatcaaca agcctagatg cgattttaga agttgaaaga ccaaaactct ggaccgtttt
     3781 aaatgacaaa cctgccttgt acgaattgat tacgcgtgtt taccgtgacg gtcaattggt
     3841 tgatgctaag aaggatttgt ttggttaccg ttactatcac tggactccaa atgaaggttt
     3901 ctctttgaat ggtgaacgta ttaaattcca tggagtatcc ttgcaccacg accatggggc
     3961 gcttggagca gaagaaaact ataaagcaga atatcgccgt ctcaaacaaa tgaaggagat
     4021 gggagttaac tccatccgta caacccacaa ccctgctagt gagcaaacct tgcaaatcgc
     4081 agcagaacta ggtttactcg ttcaggaaga ggcttttgat acttggtatg gtggcaagaa
     4141 accttatgac tatggacgtt tttttgaaaa agatgccact cacccagaag ctcgaaaagg
     4201 tgaaaaatgg tctgattttg acctacgtac catggtcgaa agaggcaaaa acaaccctgc
     4261 tatcttcatg tggtcaattg gtaatgaaat aggtgaagct aatggtgatg cccactcttt
     4321 agcaactgtt aaacgtttgg tcaaggttat caaggatgtt gataagactc gctatgttac
     4381 catgggagca gataaattcc gtttcggtaa tggtagcgga gggcatgaga aaattgctga
     4441 tgaactcgat gctgttggat ttaactattc tgaagataat tacaaagccc ttagagctaa
     4501 gcatccaaaa tggttgattt acggttcaga aacatcatca gcaacccgta cacgaggaag
     4561 ttactatcgc cctgaacgtg aattgaaaca tagcaatgga cctgagcgta attatgaaca
     4621 gtcagattat ggaaatgatc gtgttggttg ggggaaaaca gcaaccgctt catggacttt
     4681 tgaccgtgac aacgctggct atgctggaca gtttatctgg acaggtacgg actatattgg
     4741 tgaacctaca ccatggcaca accaaaatca aactcctgtt aagagctctt actttggtat
     4801 cgtagataca gccggcattc caaaacatga cttctacctc taccaaagcc aatgggtttc
     4861 tgttaaaaag aaaccgatgg tacaccttct tcctcactgg aactgggaaa acaaagaatt
     4921 agcatccaaa gtagctgact cagaaggtaa gattccagtt cgtgcttatt cgaatgcttc
     4981 tagtgtagaa ttgttcttga atggaaaatc tcttggtctt aagactttca ataaaaaaca
     5041 aaccagcgat gggcggactt accaagaagg tgcaaatgct aatgaacttt atcttgaatg
     5101 gaaagttgcc tatcaaccgg gtaccttgga agcaattgct cgtgatgaat ctggcaagga
     5161 aattgctcga gataagatta cgactgctgg taagccagcg gcagttcgtc ttattaagga
     5221 agaccatgcg attgcagcag atggaaaaga cttgacttac atctactatg aaattgttga
     5281 cagccagggg aatgtggttc caactgctaa taatctggtt cgcttccaat tgcatggcca
     5341 aggtcaactg gtcggtgtag ataacggaga acaagccagt cgtgaacgct ataaggcgca
     5401 agcagatggt tcttggattc gtaaagcatt taatggtaaa ggtgttgcca ttgtcaaatc
     5461 aactgaacaa gcagggaaat tcacccttac tgcccactct gatctcttga aatcgaacca
     5521 agtcactgtc tttactggta agaaagaagg acaagagaag actgttttgg ggacagaagt
     5581 gccaaaagta cagaccatta ttggagaggc acctgaaatg cctaccactg ttccgtttgt
     5641 atacagtgat ggtagccgtg cagaacgtcc tgtaacctgg tcttcagtag atgtgagcaa
     5701 gcctggtatt gtaacggtga aaggtatggc tgacggacga gaagtagaag ctcgtgtaga
     5761 agtgattgct cttaaatcag agctaccagt tgtgaaacgt attgctccaa atactgactt
     5821 gaattctgta gacaaatctg tttcctatgt tttgactgat ggaagtgttg aagagtatga
     5881 agtggacaag tgggagattg ccgaagaaga taaagctaag ttagcaattc caggttctcg
     5941 tattcaagcg accggttatt tagaaggtca accaattcat gcaacccttg tggtagaaga
     6001 aggcaatcct gcggcacctg cagtaccaac tgtaacggtt ggtggtgaag ctgtcacagg
     6061 tcttactagt caaaaaccaa tgcaataccg cactcttgct tatggagcta agttgccaga
     6121 agtcacagca agtgctaaaa atgcagctgt tacagttctt caagcaagcg cagcaaacgg
     6181 catgcgtgcg agcatcttta ttcagcctaa agatggtggc cctcttcaaa cctatgcaat
     6241 tcaattcctt gaagaagcgc caaaaattgc tcacttgagc ttgcaagtgg aaaaagctga
     6301 cagtctcaaa gaagaccaaa ctgtcaaatt gtcggttcga gctcactatc aagatggaac
     6361 gcaagctgta ttaccagctg ataaagtaac cttctctaca agtggtgaag gggaagtcgc
     6421 aattcgtaaa ggaatgcttg agttgcataa gccaggagca gtcactttga acgctgaata
     6481 tgagggagct aaaggccaag ttgaactcac tatccaagcc aatactgaga agaagattgc
     6541 gcaatccatc cgtcctgtaa atgtagtgac agatttgcat caggaaccaa gtcttccagc
     6601 aacagtaaca gttgagtatg acaaaggttt ccctaaaact cataaagtca cttggcaagc
     6661 tattccgaaa gaaaaactag actcctatca aacatttgaa gtactaggta aagttgaagg
     6721 aattgacctt gaagcgcgtg caaaagtctc tgtagaaggt atcgtttcag ttgaagaagt
     6781 cagtgtgaca actccaatcg cagaagcacc acaattacca gaaagtgttc ggacatatga
     6841 ttcaaatggt cacgtttcat cagctaaggt tgcatgggat gcgattcgtc cagagcaata
     6901 cgctaaggaa ggtgtcttta cagttaatgg tcgcttagaa ggtactcaat taacaactaa
     6961 acttcatgtt cgcgtatctg ctcaaactga gcaaggtgca aacatttctg accaatggac
     7021 cggttcagaa ttgccacttg cctttgcttc agattcaaat ccaagcgacc cagtttcaaa
     7081 tgttaatgac aagctcattt cctacaataa ccaaccagcc aatcgttgga caaactggaa
     7141 tcgtagtaat ccagaagctt cagtcggtgt cctattcgga gattcaggta ttttgagcaa
     7201 acgttcagtt gataacttaa gcgttggctt ccatgaagac catggagttg gtgcaccgaa
     7261 gtcttatgtg attgagtatt atgttggtaa gactgtccca acagctccta aaaaccctag
     7321 ttttgttggt aatgaggacc atgtctttaa tgattctgcc aactggaaac cagttactaa
     7381 tctaaaagcc cctgctcaac tcaaggctgg agaaatgaac cactttagct ttgataaagt
     7441 tgaaacctat gctgttcgta ttcgcatggt gagagcagac aacaaactag gaacgtctat
     7501 cacagaggta caaatctttg cgaaacaagt tgcggcagcc aagcaaggac aaacaagaat
     7561 ccaagttgac ggtaaagact tagcaaactt caaccctgat ctgacagact actaccttga
     7621 gtctgtagat ggaaaagttc cggcagtcac agcaagtgtt agcaacaatg gtctcgctac
     7681 cgtcgttcca agcgttcgtg aaggtgagcc agttcgtgtc atcgcgaaag ctgaaaatgg
     7741 cgacatctta ggagaatacc gtctgcactt cactaaggat aagaacttac tttctcataa
     7801 accagttgct gcggttaaac aagctcgctt gctacaagta ggtcaagcac ttgaattgcc
     7861 gactaaggtt ccagtttact tcacaggtaa agacggctac gaaacaaaag atttgtcagt
     7921 tgaatgggaa gaagttccag cagaaaatct gacaaaagca ggtcaattta ccgttcgagg
     7981 ccatgtcctt ggtagtgatc ttgttgctga ggtcactgta cgagtgacag acaaactagg
     8041 cgaagctctt tcagataacc ctaactatga tgaaaacagt aaccaggcct ttgcttcagc
     8101 aaccaatgat attgacaaaa actctcatga ccgcgttgac tatctcaatg acggagatca
     8161 ttcagaaaat cgtcgttgga caaactggtc tccaacacca tcttctaatc cagaagtatc
     8221 agcgggtgtg atcttccgtg aaaatggtaa gattgtagaa cggactgttg cgcaagccaa
     8281 acttcacttc tttgcagata gtggtacgga tgcaccaact aaactcgttt tagaacgcta
     8341 tgtcggtcca gagtttgaag tgccaaccta ctattcaaac taccaagcct acgacgcaga
     8401 ccatccattc aacaatccag aaaattggga agctgtgcct tatcgtgcgg ataaagacat
     8461 cgcagctggt gatgaaatca acgtaacatt taaagctgtc aaagccaaag ctatgagatg
     8521 gcgtatggag cgtaaagcag ataagagcgg tgttgcgatg attgagatga ccttccttgc
     8581 accaagtgaa ttgcctcaag aaagcacgca atcgaagatt cttgtagatg gaaaagaact
     8641 tgctgatttc gctgaaaatc gtcaagacta tcaaattacc tataaaggtc aacggccaaa
     8701 agtctcagtt gaagaaaaca atcaagtagc ttcaactgtg gtagatagtg gagaagatag
     8761 ccttccagta cttgttcgcc tcgtttcaga aagtggaaaa caagttaagg aataccgtat
     8821 ccagttgact aaggaaaaac cagtttctga gaagacagtt gctgctgtac aagaagatct
     8881 tccaaaactc gaatttgttg aaaaagattt ggcctacaag acagttgaga aaaaagattc
     8941 aacactgtat ctaggtgaaa ctcgtgtaga acaagaagga aaaactggta aagaacgtat
     9001 ctttacagcg attaatcctg atggaagtaa ggaagaaaaa ctccgtgaag tggtagaagc
     9061 tccgacagac cgcatcgtct tggttggaac caaaccagta gctcaagaag ctaaaaaacc
     9121 acaagtgtca gaaaaagcag atacaaaacc aattgattca agtgaagcta gtcaaactaa
     9181 taaagcccag ttaccaaata caggtagtgc gtcaagccaa gcagcagtag cagcaggttt
     9241 agctcttcta ggtttgagtg caggattagt agttactaaa ggtaaaaaag aagactagaa
     9301 attttgatac cttctttatc attaaagacc aaagaaaaac tcttgtccta aatagaagat
     9361 aaagagtctg gaacaaaata gttctcaacc acaaaaaagc tagatatttc caattgcgga
     9421 actctagctt tttaattttg agtcgttctc ttattgtata gtagattgaa actagagtag
     9481 tacacttcta cttctaaaac attgttagaa atcgatttga ctgtccttat cgatttgtcc
     9541 tgttcttatt tcatttcact atgttttagg aggttattgg ccataagtac caatcccatg
     9601 tcaattctca cttgacgctt gcctctcaga ttacatctct tgtaacccaa acaagccttt
     9661 atctgcccaa agacaggttc cacatcaatc ttgcgttgag cgaaaatctg tctatcttgg
     9721 ggagataaaa gcgcctgaca ttctttagct ttcaagtttt gatagcgttc gttcatatac
     9781 agtccctttt gaggggctga ttcaggttca tcagcgtagt aaaccttgat ttcctgttga
     9841 aagtctgtct gtgttttctg gtacttgata tggtgaaaac gatagcacca gccatcagga
     9901 tgtatgtagc tatcctcctt gtcattatag tgccaattcg ctaagttttt agctgactgt
     9961 ttataccctc tcttttgttt cttatcaaac atggcatatt taatcagaag gggagtgatt
    10021 tcttcttgaa aatagacttg tatctgttct tgaagtttgg cggagaattt ctctgtcgtt
    10081 ttcttccaca caaaactata cttgttggca ttggcttcaa tcttagttcc gtcaataaac
    10141 aaacaatcta aggtcactaa ctcttccatt tttcaatttt tcgaccagag aaaatccctt
    10201 gtgaataggc aaatagaaga gtagatacaa gcattttaag gtgataagac gggcgatcaa
    10261 aggcatgata gaaggcgtgg aaatgacaat cctccaaggt attcaccact ttttcaatag
    10321 taaaaacgag atggtcttgt ggcaagaagg aactgatttc tagtggtaaa gttgtttgat
    10381 ttgtgttata gtgaatatgc atgggatagc ctttctaaat ggtttattgt gcaattctat
    10441 tttaccaaga ctatcgcaac catgcaaaaa agcaacggca aatccgttgc tttttttgga
    10501 gataagagac tattgtccca gactcttttc tttgtataaa attgtatagt gttattgtct
    10561 atactatttt gtagagaaag caaaaatccc tagaaaatgc ttagggattc gactatttca
    10621 ttaagaacaa ttcaacaatc atttctacat acatgataag ggttaataag aatcctgcac
    10681 gccagaagaa tttgatgaat ttaggatagt aaaagctgcg ttttttcaaa aggaagaaaa
    10741 agacgagaat aatggctagg atcgagaggg ttagccccag tctagggagg aaattatggg
    10801 taaaggtttt agctgtaatc aggtaatatt caaataccaa gaccggaaaa gccaaatccg
    10861 caaagtttcg tcctagtttt tttaatctaa aaagtttggt taggataatg cagacgacta
    10921 aggtcagtat caataataaa atagatgcta atttcattaa aatcataccc atattgtatc
    10981 ataaaaaaag gctaatggaa acgagaaaca gaggaattta taggaaagtc aggcttttga
    11041 gattgtgggt gatttttgtt ataatgaaag tcatgaaatc ttataatacc ttgaatgatt
    11101 attatcgaaa actctttgga gaaaagactt ttaaagtccc tattgatgcg ggatttgact
    11161 gtcccaatcg tgatggaact gtggctcatg gaggcagtac tttttgtacg gtttcaggtt
    11221 ctggagatgc tattgtagcc ccagatgcgc ctatccgtga gcaattttat aaggaaattg
    11281 actttatgca ccgtaagtgg ccggatgttc agaagtatct ggtttatttt caaaatttta
    11341 ccaacaccca tgaaaaggtg gaagttattc gagagcgtta tgaacaggct atcaatgagc
    11401 caggtgtggt aggtatcaat attggaacgc gcccagactg tttaccagac gaaaccatcg
    11461 aatatttggc tgagttatcg gagtgcatgc atgtgacggt tgaattgggc ttgcagacaa
    11521 cttatgaagc aacctctgac ctgattaacc gtgtgcattc ttatgaattg taggtggaaa
    11581 cggtcaagcg tttgagaaag tatcccaaga ttgagattgt ttcccatttg atcaatggtt
    11641 tgcctggtga aacccatgag atgatggttg aaaatgtccg ccgttgtgtc acggataacg
    11701 atattcaagg gattaaactg catttgctcc atcttatgac aaatactcgt atgcaacgag
    11761 attaccatga gggacgttta caattgatga gtcaggacga atatgtcagg gtcatctgtg
    11821 accaactgga aattattccc aaacatatcg tcatccatcg aattacagga gatgcgccta
    11881 gggatatgct gattggtcct atgtggagcc tcaaaaaatg ggaagtcctc aacagcattg
    11941 agatggagat gcgacgtcgt ggaagtgttc aaggatgcaa ggctgtaaaa caggagtttg
    12001 aaaatgaaaa gaccacttga gatggcacat gattttttgg ctgaggtcgt gacaaaagag
    12061 gatgtcgtag tggatgcgac tatgggaaat ggtcatgaca cgcttttttt agccaagcta
    12121 gccaagcaag tctatgcctt tgatattcag aagcaagcct tggaaaagac ccaagagcgt
    12181 ttgcatcagg ctggcttgac aaatgcccag ttaatcttgc aaggccatga gacactggac
    12241 cagtttgtga caaaagctaa ggcagggatt tttaatctgg gctatttgcc gtcagctgat
    12301 aagtctgtca tcacccgacc gcagacaacg attgaggcat tagaaaagct atgtggctta
    12361 cttgtcaaag gtggacgaat tgctattatg atttactatg gtcatgaagg aggcgacctc
    12421 gagagagatg ctgtcttgga ttttgtaatc cagttgaacc aacaagagta cacagctgcc
    12481 atttaccgaa ctttaaatca agtcaacaac ccgccgtttt tagtgatgat tgaaaaatta
    12541 gagagatata gacatggata aacaatacct acgtgaaaag ctggatgcca tgcgccagaa
    12601 ttttgttgaa tcaacccacc acgaacgagc ggtgggcgtg ctagaccaag cgcatatgag
    12661 caaaaaaatg cttaaaatca agaaaaaatt agttgctctt gaaatggaac ggtgccagag
    12721 aaaaattgag cacaaagact gttccaagat tgaccaaaaa atcaaagagc agaaggagat
    12781 atttgaatcc tgttgtaaaa aagattaagg aggacgtgcg tggaattact gatttacctc
    12841 atcctatttt tactggtctt gattgtctca agtacaacca ataagctcct gccctttttg
    12901 cctctccctt tggtgcagat tcttttggga attgtgattg gtctcttttt acccaatact
    12961 gactttcatc ttaatacgga gttgtttttg gccctggtta tcggaccctt gcttttccga
    13021 gaggctgaag aagcagatgt tacggctatt ttaaaacact ggcgaatcat tgtttatctc
    13081 atatttccag tgatttttat ctcgaccctg agtttgggtg gcttggccca tcttctttgg
    13141 ttcagccttc ccttggcagc ttgcttggct gttggggcag cccttggtcc tacggacttg
    13201 gtggcctttg cctctctttc ggagcgtttt agctttccta agcgcgtgtc caatattctt
    13261 aagggcgaag gactcttgaa tgatgcttct ggtttggtgg cttttcaggt agctttgaca
    13321 gcttggacaa ctggagcttt ttctctgggg caagctagca gttcgctcat cttttcaatc
    13381 ctaggcggtt ttttaattgg atttttaaca gccatgacca accgcttcct ccataccttc
    13441 ttgctaagtg tgcgcgcaac ggatattgcc agtgaacttt tattagaatt gagtttgcct
    13501 ctagtgacct tctttctggc agaagaagtc catgtttcag ggattattgc cgtcgtagtt
    13561 gctggaattt taaaggcaag tcgcttcaag aaaatcacgc tcctcgaagc ccaagtggat
    13621 acggtgaccg agacggtctg gcatacagtg acctttatgc tcaacggttc tgtctttgtg
    13681 attttaggga tggagttgga aatgatagca gaacctatct tgaccaatcc aatctataat
    13741 cctctacttt tattgctatc tctcatcgcc cttacctttg tcctctttgt cattcgtttt
    13801 attatgatct atggctatta tgcctataga acccgacgcc taaagaaaaa gctaaataag
    13861 tatatgaagg acatgtttct cttgaccttt tcaggtgtta agggaacggt gtcgattgct
    13921 acgattctct tgataccaag taatctagaa caggagtatc ctctcttgct tttccttgtt
    13981 gcaggtgtga cgcttgtcag ctttttaaca ggtctcttgg tcttgcctca tctttctgat
    14041 gaagaggaag aaagcaagga ttatctcatg catatcgcca ttttgaatga agtaacgcta
    14101 gagttggaaa aagagttgga agacaccaga aataaacttc ccctctatgc ggctattgac
    14161 aattatcatg gacgtattga aaatctcatt ttaagccaag aaaaccagga tgatcaagaa
    14221 gactgggctg ctttgaaact cttaattctt agtattgaaa gtgatggttt ggaacaggcc
    14281 tatgaagagg ggaacattag caatcgtgct taccgagttt accaacgtta tctgaaaaat
    14341 atagaacaag gaatcaatcg taaacttgcc tcaagactga cctattattt tcttgtttcc
    14401 ttgaggattt tacgttttct tcttcatgaa gtttttactc ttggaaagac cttccgtagc
    14461 tggaaggaca aggagcaaag ccgtctccgt gctcttgatt atgaccaaat tgcagagctc
    14521 tatcttgcca atacagagat gattattgaa agtttggaaa acctgaaggg agtctacaga
    14581 cgctctttga ttagttttat gcaggagtct cgtcttcgag aaacagctat tatcagcagt
    14641 ggtgcctttg tcgaacgggt tatcaatcgt gtcaaaccca acaatatcga tgaaatgctg
    14701 agaggctatt atctggagcg caagttgatt ttcgaatacg aagaaaaacg attgattacg
    14761 actaagtatg ccaagaaatt acgacaaaat gtaaataact tagagaacta ttccttgaag
    14821 gaagctgcca ataccctgcc gtatgatatg gtggaattgg taagaagaaa ttagttaata
    14881 ctcttcgaaa atctcttcaa accacgtcag cgtcgccttg gattatatat gtgactgact
    14941 tcgtcagttt catctacaac ctcaaagcag ggctttgagc aacctgcggc tagcttccta
    15001 gtttgctctt tgattttcat tgagtataag attgtaagtg aaggagtgtg acatgaaaaa
    15061 atggtggaaa gagctgatag acaagccttt attaaaagct tttttgcatt attatcaagc
    15121 atcagatagt gagttgacca gtgtcgcagt agcctactat tggttgattt cgattttccc
    15181 cctgcttttg gtggtggtca atatcctccc ttattttcag attcctgtgg gagaatttct
    15241 gggctttatg aaagacgtcc tgcccccaag cctctatgaa ggtgtggaaa aaatagccag
    15301 agaagtcttg acgcaacctt caacaggttt attgagtttt tctgttttat cggcgttatg
    15361 gagtttttca aaatctatga atttcttgca aaaagctttt aacaaggctt atggcgtcga
    15421 aaagagtcgt ggacttattt cccatcagat gctgagtctc ctagtcagtt ttggcttgca
    15481 gctcctcttt gcgtttgcct tgtttttgat cttatttggt cagatgattt tggctttact
    15541 tgctcattat tggacaaaag atggcattat ctatcaggct ttacaagggt tggcaggtcc
    15601 tctgatttac gccctcctct ttgctatctt ggtcatgctt tattattttc ttcccaatct
    15661 atctaacagg aaaattagct ataccctacc aggcagtgcc tttgttctct tagtgatttt
    15721 aggtttacta actctgttct ctagttatct caattactat gtccatcatt tggtggatgt
    15781 ccgaatttta ggttccgttc ttcttgttgt tatgatgttt tggtttatct tgattgctaa
    15841 aattgttatc ctaggcgcgg ttatcaatgc cagtatgcag agtttgaaag atccggtctt
    15901 taaaaaagac taactattta tccattataa aagcgcatag tatcaagact ttatagaacc
    15961 ttgatactat gcgcttttgc gcttttgtgc tttgacagtt ttttgtatag tttttgtcca
    16021 agatttctaa atcatctgat taaattccta ttttccccta tcattgtccc tgttttttcg
    16081 aaaatggaag gagtataata aatctatcaa taaaatgata ggaggaactt tatgggtcat
    16141 attttctttt ttctaagtgt ctttttggca gggattctat ccttcttttc tccttgtatc
    16201 ttacctttgt taccggtcta tacaggagtg ttactagatg ataaggatgg tgctcaggct
    16261 tctagcggca aattttcaat ctcagttact agtttattac gaactctggc ctttatagca
    16321 ggaatttcct ttatatttat tttgttgggc tatggagctg gttttttagg cgatttgctt
    16381 tatgcttctt ggttccaata tcttactggg gcaattatta tccttcttgg tttgcaccaa
    16441 atggagattc tacactttaa ggggctttat aaggaaaaga ggctacaact gcaaggacag
    16501 gggcaaaatg gtaagggcta tagtcaggca tttttattgg gcttgacctt tagttttgct
    16561 tggacgcctt gcgtggggcc ggttctgggg tctgttttgg ccttggcggc ttcaggtggt
    16621 tcaggagctt ggcagggagc tggtctcatg ttggtgtata cgctgggctt ggcgctacca
    16681 ttcttgcttc tagctctgac ctctagttat gttttgaaac atttccgaaa acttcatccc
    16741 tatctcggaa tcctcaaaaa agtgggtggt tttctcatta ttgtgatggg actcttggtt
    16801 ctgtttggaa atgcttcaat tttaagtcaa ttatttgaat aaaatggaaa ggaatatcaa
    16861 tatgaaaaaa tggcaaacat gtgttcttgg agcaggttcg ctcctttgtt tgacggcttg
    16921 ttcaggcaag tccgtgacta gtgaacacca aacgaaagat gaaatgaaga cggagcagac
    16981 agctagtaaa acaagcgcag ctaaagggaa agaggtggct gattttgaat tgatgggagt
    17041 agatggcaag acctaccgtt tatctgatta caagggcaag aaagtctatc tcaaattctg
    17101 ggcttcttgg tgttccatct gtctggctag tcttccagat acggatgaga ttgctaaaga
    17161 agctggtgat gactatgtgg tcttgacagt agtgtcacca ggacataagg gagagcaatc
    17221 tgaagcggac tttaagaatt ggtataaggg attggattat aaaaatctcc cagtcctagt
    17281 tgacccatca ggcaaacttt tggaaactta tggtgtccgt tcttacccaa cccaagcctt
    17341 tatagacaaa gaaggcaagc tggtcaaaac acatccagga ttcatggaaa aagatgcaat
    17401 tttgcaaact ttgaaggaat tagcttagga ggtgtcttat gaatgataag ttaaaaatct
    17461 tcttgttgct aggagtattt tttctagcca taaccggttt ctatgttcta ttgatacgaa
    17521 atgcagggca gacagatgcc tcgcaaattg aaaaggcggc agttagccaa ggaggaaaaa
    17581 cagtgaaaaa aacagaaatt agtaaagacg cagacttgca cgaaatttat ctagctggag
    17641 gttgtttctg gggagtggag gaatatttct cacgcgttcc cggggtgacg gatgccgttt
    17701 caggctatgc aaatggtaga ggagaaacaa ccaagtacga attgattaac caaacaggtc
    17761 atgcagaaac cgtccatgtc acctatgatg ccaagcaaat ttctctcaag gaaatcctgc
    17821 ttcactattt ccgcattatc aatccaacca gcaaaaataa acaaggaaat gatgtgggga
    17881 cccagtaccg tactggtgtt tattacacag atgacaagga tttggaagtg attaaccaag
    17941 tctttgatga ggtggctaag aaatacgatc aacctctagc agttgaaaag gaaaacttga
    18001 agaattttgt ggtggctgag gattaccatc aagactatct caagaaaaat ccaaatggct
    18061 actgccatat caatgttaat caggcggcct atcctgtcat tgatgccagc aaatatccaa
    18121 aaccaagtga tgaggaattg aaaaagaccc tgtcacctga ggagtatgca gttacccagg
    18181 aaaatcaaac agaacgagct ttctcaaacc gttactggga taaatttgaa tccggtatct
    18241 atgtggatat agcaactggg gaacctctct tttcatcaaa agacaaattt gagtctggtt
    18301 gtggctggcc tagttttacc caacccatca gtccagatgt tgtcacctac aaggaagata
    18361 agtcctacaa tatgacgcgt atggaagtgc ggagccgagt aggagattct caccttgggc
    18421 atgtctttac ggatggtcca caggacaagg gcggcttacg ttactgtatc aatagcctct
    18481 ctatccgctt tattcccaaa gaccaaatgg aagaaaaagg ctacgcttat ttactagatt
    18541 atgttgatta atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtatg
    18601 tagggttact gactccgtca gttttatcta caacctcaaa gctatacttt gagctgactt
    18661 cgtcagtttt atctacaacc tcaaagcggt actttgagaa acctgtggct atcttcctag
    18721 tttacttttt gatttttatt gagtataaga ggtctttcct aagcagttag aggagaattt
    18781 tgctatactg atactagtaa gtggtaaagg agcagagcat gacctacaca atcttaatcg
    18841 tagaagatga atatctggta agacaaggtt tgactaaact ggtcaatgta gcagcctacg
    18901 atatggaaat catcggtcag gctgaaaatg gaaggcaggc ttgggaattg atccaaaagc
    18961 aggttccaga tatcatttta accgatatca acatgcctca tctaaatggc atccagttgg
    19021 ccagtctggt acgagaaacc tattctcagg ttcatttggt ctttttaaca ggttacgatg
    19081 attttgatta tgccttgtct gctgtcaaac taggtgtgga cgactacctg ctcaaaccct
    19141 tttctcgtca ggatattgag gaaatgttgg ggaaaatcaa acaaaaacta gacaaggaag
    19201 agaaagaaga gcagttacaa gatttattga ctaataggtt tgaaggaaac atggcccaga
    19261 aaatccagtc tcatctggct gatagccaat ttagtttaaa gtctttagcc agtgacttag
    19321 gttttagtcc gacctatctg agttccttga ttaagaaaga gttgggcttg ccttttcagg
    19381 attatctagt gagagaacgt gttaaacaag ccaagctctt gcttttaact acagatctga
    19441 agatttatga gatcgcagag aaggttggtt ttgaagatat gaactatttt acccaacgtt
    19501 ttaagcagat tgcaggtgtg acacctcgtc agtttaagaa gggagaagac cgatgaagcg
    19561 ttcttctctt ttagttagaa tggttatttc catctttctg gtctttctca ttctcctagc
    19621 tctggttgga actttctact atcaatcaag ttcttcagcc attgaggcca ccattgaggg
    19681 caacagccaa acgaccatca gccagactag ccactttatt cagtcttata tcaaaaaact
    19741 agaaaccact tcgaccggtt tgacccagca gacggatgtt ctggcctatg ctgagaatcc
    19801 cagtcaagac aaggtcgagg gaatccgaga tttgtttttg accatcttga agtcagataa
    19861 ggacttgaaa actgttgtgc tggtgaccaa atctggtcag gtcatttcta cagatgacag
    19921 tgtgcagatg aaaacttcct ctgatatgat ggctgaggat tggtaccaaa aggccattca
    19981 tcagggagct atgcctgttt tgactccagc tcgtaaatca gatagtcagt gggtcatttc
    20041 tgtcactcaa gaacttgttg atgcaaaggg agccaatctt ggtgtgcttc gtttggatat
    20101 ttcttatgaa actctggaag cctatctcaa tcaactccag ttggggcagc agggctttgc
    20161 cttcattatc aatgaaaacc atgaatttgt ctaccatcct caacacacag tttatagttc
    20221 gtctagcaaa atggaggcta tgaaacccta catcgagaca ggtcagggtt atactcctgg
    20281 tcacaaatcc tacgtcagtc aagagaagat tgcaggaact gattggacgg tacttggcgt
    20341 gtcatcattg gaaaagttag accaggttcg gagtcagctc ttgtggacct tgcttggggc
    20401 cagtgtcaca tctcttcttg tctgtctctg cttagtgtgg ttcagtctta aacgctggat
    20461 tgctcctttg aaggatttga gagaaaccat gttggaaatt gcttctggtg ctcaaaatct
    20521 tcgtgccaag gaagttggtg cctatgaact gagagaagta actcgccaat ttaatgccat
    20581 gttggatcag attgatcagt tgatggtagc tattcgtagc caggaagaaa cgacccgtca
    20641 gtaccaactt caagcccttt cgagccagat taatccacat ttcctctata acactttgga
    20701 caccatcatc tggatggctg aatttcatga tagtcagcga gtggtgcagg tgaccaagtc
    20761 cttggcaacc tatttccgct tggcactcaa tcaaggcaaa gacttgattt gtctctctga
    20821 cgaaatcaat catgtccgcc agtatctctt tatccagaaa caacgctatg gagataagct
    20881 ggaatacgaa attaatgaaa atgttgcctt tgataattta gtcttaccca agctggtcct
    20941 acaacccctt gtagaaaatg ctctttacca tggcattaag gaaaaggaag gtcagggcca
    21001 tattaaactt tctgtccaga aacaggattc gggattggtc atccgtattg aggatgatgg
    21061 cgttggcttc caagatgctg gtgatagtag tcaaagtcaa ctcaaacgtg ggggagttgg
    21121 tcttcaaaat gtcgatcaac ggctcaaact tcattttgga gccaattacc agatgaagat
    21181 tgattctaga ccccaaaaag ggacgaaagt tgaaatatat ataaatagag tagaaactag
    21241 ctaactccca gtcaattctg ggagttttat gcgtaattgg gcaaacttta taggttgtct
    21301 atcttgctaa aaacaagaaa aaacggtatg atagaagagt gacaacagag gtaacagaga
    21361 tgaacgagac acagattcaa agagaaacac gtcaggtagt ggaagatgtt ttagaaaaga
    21421 ccaatttgaa gcagggggtc ctttttgttt taggtctatc ttctagtgag gtgctaggtg
    21481 gtcagattgg gaaagaatcc agtcaagaga ttggtgagct gatagtggag actatcttgg
    21541 gtattctagg tagtagaggt atccatttag cagttcaagg ttgtgagcat gtcaatcggg
    21601 ctctcgttgt tgaacgtcag gtggcagagc agtttgatct ggaaattgtc agtgtccatc
    21661 ctactcttca tgcaggaggt tcggggcagt tggcagcctt taagtttatg caggatccag
    21721 ttgaggttga atttatcaag gctcatgctg gattggatat cggagacact gcaattggca
    21781 tgcatgtcaa gcatgttcag gttccgattc gccctatttt gagagagatt ggtcatgccc
    21841 atgtaacggc actggctagt cgtccaaaat taatcggagg tgcgcgtgcg cactatccgc
    21901 aagacgctat tagaaagtct tgagaatgaa taaaggagat aaaagtgttt aaaaaagacc
    21961 gtttttcaat tcgtaagatt aagggagttg taggctctgt atttcttgga agccttttga
    22021 tggctccttc tgtagtggac gcagccacct atcactatgt aaataaagag attatttcac
    22081 aagaagctaa agatttaatt cagacaggaa agcctgacag gaatgaagtt gtatatggtt
    22141 tggtgtatca aaaagatcag ttgcctcaaa cagggacaga agcatctgtt ttgacagctt
    22201 ttggtttgct gactgttggg agcttgcttt taatctacaa gagaaagaaa attgctagcg
    22261 tctttctagt tggagctatg ggattggtag ttcttcctag tgcaggggct gtagacccag
    22321 ttgcgaccct agcgctggct agtcgagagg gtgttgttga aatggatggc tatcgctatg
    22381 ttggttatct atcaggtgac atcctcaaaa cgcttggctt ggacactgtt ttagaagaaa
    22441 cctcagcaaa acctggagag gtgactgtag tcgaagttga gactcctcaa tcaacaacaa
    22501 atcaggagca agctaggaca gaaaaccaag tagtagagac agaggaagct ccaaaagaag
    22561 aagcacctaa aacagaagaa agtccaaagg aagaaccaaa atcggaggta aaacctactg
    22621 acgacaccct tcctaaagta gaagagggga aagaagattc agcagaacca tctccagttg
    22681 aagaagtagg tggagaagtt gagtcaaaac cagaggaaaa agtagcagtt aagccagaaa
    22741 gtcaaccatc agacaaacca gctgaggaat caaaagttga accaccagta gaacaagcaa
    22801 aagtcccaga acaacccgtg caacctacac aagctgagca accaagtaca ccaaaagaat
    22861 catcacaaca agaaaatcct aaagaagata ggggagcgga agagacaccg aaacaagaag
    22921 atgaacagcc agcagaagcc ccagaaatca aggttgaaga accagtagaa tcaaaagagg
    22981 agactgttaa tcaacctgtt gaacaaccaa aagtggaaac gcctgctgta gaaaaacaaa
    23041 cggaaccaac agaggaacca aaagttgaag taacaagtat tccccaaact actcgctatg
    23101 aggaagacct tactaaggaa cacggaacgc gtgaagttgt taaggaaggt aagaatggca
    23161 gtagaacagt tactactcca tatatcttga atgcgacaga tggtacgact acagaaggca
    23221 cttcgacaac tgatgaagct gagatggaga aagaggttgt tcgtgttggc acgaaaccca
    23281 aagaaaaatt agctccagtc ttaagtttga caagtgttac agataatgca atgttgcgta
    23341 gtgcgagact tacttatcat ttggaaaata cagatagtgt tgatgtgaaa aaaattcatg
    23401 ctgaaattaa aaatggcgat aaggttgtca aaactattga cttatctaaa gagagattat
    23461 cagatgctgt tgacggtctt gaactttata aagattataa gattgtgacg agtatgacct
    23521 atgatagagg taatggtgaa gaaacctcta cgttggaaga aactccacta cgattagacc
    23581 tcaagaaggt tgaattgaaa aacatcggct ctactaatct cgtcaaagta aatgaggatg
    23641 gtactgaggt ggcaagtgac ttcttaacaa gtaaacctgt ggatgtgcag aattactacc
    23701 tcaaagtaac ttcacgtgat aataaagtgt ttcgcctcac agttgaaaaa attgaagagg
    23761 tgactgagga aggtcaacca ctttacaaag tcactgctaa ggctcctaat ttgattcagc
    23821 acacggatgc gactaaaatg caagacgaat acgtgtatta tattgaaaaa acacgtgcaa
    23881 cagatggtga tatttactac aactttaacg acttagtaaa cgccatgaac aaaaacaaaa
    23941 ctggtacatt caaacttggt gctgatttga acgctacagg tgtaccgaca cctgctaagt
    24001 cgtatgttac aggtgatttc cgaggtactt tgacaagtgt tgatggtgaa cattacacaa
    24061 ttcataatac aagtcgccca ttgtttaata atattatagg tgggactatc aaagatatta
    24121 accttggcaa tgttaatatt cacatgccat gggcaaataa cgttgcatcg ttagctaata
    24181 ttattaaagg tggaaccact attgaaaatg tcaaagttac aggtaatgtt cttggtaaag
    24241 actgggtatc tggatttata gataaaatag atagtggcgg tacattgaga aatgtagctt
    24301 ttattgggaa tgtgacctct gttggtacag gtggttcgtt cttgactggt attgttggtg
    24361 aaaactggaa gggactggta gaacaggctt atgtagatgc caatattcga gggaagaaag
    24421 ctaaggctgc aggtatagct tactggtcgc aaaatggagg cgataactat gctgtaggta
    24481 gatatggagc aattaagaaa tctgtcgtta aaggttcaat agatgtagaa aaaccaattg
    24541 aagttggtgg cgctgttgga agtttaaatt atcttggtta tatagaagac actgttgcta
    24601 tgatgaaagt taagaacggt gaaattttct acggttcgca tgatatcgat accgatccat
    24661 attacacagg agaacgagta aatagaaatt tcattgttga cggtgtaagt gaaggtaaat
    24721 cttcgtataa atactctaaa caacaaaatc gcattaaatc agtatctcaa gaggaagctg
    24781 ataagaaaat taaagaattg gctatcacag cagataaata tgcaattaca gagccaattg
    24841 taaataaact caacgctctc acaacacgtg ataatgagta tagaacaaca caagattaca
    24901 aagcagaccg tgaacttgct tatcgtaata tcgaaaaact tcaaccattc tacaataaag
    24961 agtggattgt agaccaaggt aataaagttc catcaaattc taaattatta actacagaag
    25021 tattatctgt tacaggtatg aaagatggtc aatttgtgac tgacttatca gaaattgata
    25081 aaattatgat tcactatgcg gatggcacca aagaagaaat gaacgtgaca gcggttgctg
    25141 attctaaggt gaaacaagtt cgtgagtatg acgtgactga tttgggtgtt gtttacactc
    25201 cgaatatggt tgataaaaac cgcgaccaat tgattgcaga tgtgaaagcg aaattgtcgt
    25261 cagttgaatt gatttcccct gaagttcgtg ctcttatgga taaacgtggt aaagctgaag
    25321 aaaatacgga aggtcgtcaa aatggataca ttagagatct attccttgaa gaaagctttg
    25381 ctgaagtgaa agctggtttg ggtaaattgg ttaaagcttt ggttgaaaac gaagattatc
    25441 aattaaatag cgatgaagca gcaatgcgcg cgttaattaa gaaagttgaa gacaataaag
    25501 ctaaaatcat gatggaactg gcctacttaa atcaatatta tagttttaaa tacgctgagt
    25561 tatcaattaa agatattatg atgtttaaac ctgattttta tggtaagaat gttaatgtct
    25621 tagatttctt aatcaaaatt ggttcaagtg aaagaaatgt taaaggtgat agaactttag
    25681 aagcttatcg tgaaactatc ggtggaacaa ttggaataaa tgaattaaat ggattcttac
    25741 attacaatat gaagctattt actaaccata tagatataaa tgactggttt aagaaagcta
    25801 ttgaaaagaa tgcttatgta gttgaacaac catcaacgaa ccctgcattt gccaacaaaa
    25861 aatatcgtct atacgaagga attaataatg ggcaacatgg gcggatgatt ttacctcttc
    25921 ttaacttgaa aaatgcacat ctatttatga tttcaacata caacactatc tcgtttagtt
    25981 cgtttgaaaa atatggcaaa gacacagatg aaaaacgtga aaagtttaag agtgagatca
    26041 ataaacgtgc taaagagcag gttaattact tagatttttg gtcacgtctt gctactgata
    26101 atgtccgtga taaacttctt aagagtcaaa atgtagttcc aactcctgta tgggataatc
    26161 ataatagccc gaacggatgg gctagtagac atggtcatat agatgggaaa ccggattatg
    26221 ctccaattcg tgagttcttt gggcgaatca ataagtatca tggttacaaa tatggatatg
    26281 gcgcatatgc atatattttt gcagcacctc aaccgatgga tgcagtttat ttcgttatga
    26341 cagatttaat tagtgacttt ggaacatctg catttacaca tgaaactact cacattaatg
    26401 atcgtatggc ttactatggt ggtcattggc atcgtgaagg tacggatctt gaagcatttg
    26461 ctcaggggat gttacaaacg ccatcggtat caaatccaaa cggagaatat ggcgcattag
    26521 gtctaaatat ggcgtatgaa cgtcaaaatg atggaaatca atggtataat ccaaatccta
    26581 acaaacttaa atctcgcgct gaaattgatc actacatgaa gaactacaat gaagctctta
    26641 tgatgttaga ttatctggaa gcagaatctg ttttacctaa attaaaaggt aacaatgata
    26701 gatggttcaa gaaaatggat aaacaaatgc gtaaggacgg acaacctcac cagtttgaca
    26761 aaattcgcga tttaaataat gaagagaaga aaattcaatt agcttctatt gaggatttag
    26821 ttgataataa ctttatgact aaacacggtg ctcctggaaa tggtacatat aacccatcag
    26881 attttagttc agcatatgtt aatatgaata tgatgactgg tgtatacgga ggtaactcaa
    26941 gtgatggggc tcctggcgct gcttcattta aacataatac tttccgtatg tggggatact
    27001 tcggatacga aaatggattc atcggatatg cctctaataa atataaagcc gaagccaata
    27061 aagcaggtca aactctaagt gataaatata tcatcaataa agtttctggt ggtacattta
    27121 atacacttga agcgtggaag aaagaatggt ttaaacaaat caaaactaaa gctcaaaaag
    27181 gattcacagc aattgaaatt gatggtaaaa ctattgattc atatgaaaaa ttaaaagact
    27241 tatttgataa agctgttgaa gaagatctta aaggaactgg aacggataag actgtgaaac
    27301 ttaaagaaaa agtatacaaa caactcctta aaaacacaga cggtttctct ggtgatctat
    27361 ttactgcacc acaagcatag aatgaataaa atcggaaaaa ggtagatcag ctttgtctgt
    27421 atctgccttt ttatgctata cttaaggtat gcatagaaaa acagtgattg attttaggac
    27481 tttaggggag agatatacct ttacccagcc tattaaagag ttgaaaacga gaaatgtagc
    27541 agaagtggca gatttgctgg cacaagtgga aagctaccaa gagcaagatt attatgtggt
    27601 ggggtatgtc agctacgagg ctgcacctgc ttttgaggag aaattagcag ttcacaaggt
    27661 tcctctactg ggcgagtact tgctttactt tactgttcac gatagggtgg agacctcccc
    27721 tattcctctg acttatgagg atattgattt gccctcaaat tggcaggaag taacgtctgc
    27781 agagaactat gaaaaggcca ttgcccagat acaccatcac ttgaggcagg gagacaccta
    27841 tcaggtcaac tacaccgtcc aactcaagca aaagttaagt gccaatcctt ttgccatcta
    27901 caatcgtatg gtggtagagc aggaggcggg ctacaatgcc tatgtggaac atgacgagat
    27961 ggcagtgatt tccatgagcc cagagctctt ttttgagcaa aatgatcgcg agttgacaac
    28021 acgaccaatg aaggggacga ctcagcgtgg ggtaactgac caagaagatc ttgaacaggc
    28081 cagttggttg ggacaggatc ccaaaaatcg ctctgaaaat atgatgattg tggacctctt
    28141 gcgcaatgat atgaaccgta tttctgaagt tgggagcgag cacgtggagc gtctgtgtca
    28201 ggtagagcag tattcaactg tttggcagat gacttcgacc atcaagagtc agttgcgaga
    28261 ggatgtggac cttgttgaaa tcttccgctc actctttcct tgtggttcca taacgggtgc
    28321 accgaaaatt gcgacaatgg agattatcaa ggacttggag cctcaaccgc gtggagtcta
    28381 ctgtggaacg attggtctct tgcttccaaa tggacgacgg atttttaatg tggccattcg
    28441 taccattcaa cttcacaaag gtcaagcaat ttatggagtt ggcggaggga ttacttggga
    28501 tagcacatgg gaatctgaat accgagaggt tcatcaaaag gcagcagttc tttatcgtaa
    28561 acaagctcgc ttccaactga ttacgacagg ggaaatcagc cagaagaacc tgctgtttga
    28621 agatcaacat ctggaaagac tgagaaaagc cagtcgttat tttgcctttc cttttgatgc
    28681 agaagacttg ggacacaaga ttgaggaaga gtgtcaggat tgtgaagcta atcaagatta
    28741 ccgcttgcga atcagcctta gcaaatctgg agagatagaa gtcaatcgtc aagtattaac
    28801 ccctctcagt acaagctttt gtcaggccca agtctgtctt caggaagctg ctttgaatca
    28861 atcctttacc tactttaaaa ccactcaccg accgcatttg agcctaggag aacaagagaa
    28921 gatttaccac aataagtcag gagaactgct tgaaacctct ataggaaatt tggttctgaa
    28981 aatcgctgga aaactctaca caccgcctat ccgacttgga atcttgccag gaatttaccg
    29041 tcagcatttg ctagaaacag gacaggtaga agagaaagtc ttgaccttgg cagatttaac
    29101 ccaagcagaa gctatttacg gctgtaatgc agtgagaggc ttgtatgaac taagccttga
    29161 ggagaactag atgaaattga tatttttgca tgggttaggg cagtcagcag agtcttggaa
    29221 agaagttcgg aatctactga cagattatcc ttctgaggct atcgagttat ttccttctgg
    29281 agttagtaac tatcaacaag ctaaagagcg agtttatcag cacctagctc aagagacaga
    29341 accttttgtt ttgattggat tgtccttagg tgctaccctg acactagaac tttccagtta
    29401 cgatttacca aatcttcgag ctttgattct gtcaggatgc ccgcttaaat tagcagggaa
    29461 tatcctattt tatcttcaat tgctgatttt taaactactt cccaaaagag tatttgaaaa
    29521 acagggagca gacaagactc ttatggtcgg agtttctgag gaattgaaga cacttgattt
    29581 aacggatata gcagggactt gctcctatcc aaccttacta atttgtggca gcaaagataa
    29641 accgaatctt agttctatga aagctctcca tagactgtta acagattcgc agtttcagat
    29701 cattcctgat ggccctcatg tcttgaataa ggcaaaacca aaggagtttg tagaaaagac
    29761 cagaagtttc cttgaattgc tgaaataagt ttgataaaat aatattgaaa tcgatatcat
    29821 aattatggga gaaagaaatg aataaacgtc tatttttaaa aatgagtctg gtgacgttgc
    29881 caattttagc cttgttttca caaccagttt tggcggaaga aaacatccat ttttcgagct
    29941 gtaaggaagc ttgggcgaat ggctattcgg atatttacga gggagaacct ggttattctg
    30001 ccaagttaga ccgtgatcat gatggtgtgg cttgcgaatt gaaaaatgct cctaagggtg
    30061 cttttaaagc aaaacagtca acggctattc aaatcaacac aagttcagca acaacaagtg
    30121 gttgggttaa gcaggacggc gcttggtact actttgatgg aaatggaaat ctagtgaaaa
    30181 atgcatggca gggaagctat tacctgaaag ctgatggtaa aatggcacag agtgaatgga
    30241 tttatgactc ttcttatcaa gcttggtatt atttgaaatc agatggttct tatgcaaaaa
    30301 atgcatggca aggagcttat taccttaaat caaacggtaa aatggtacaa ggtgagtggg
    30361 tttatgattc ttcttaccaa gcatggtatt acttgaaatc agatggttca tatgctcgca
    30421 atgcatggca aggaaactac tatttgaaat cagatggtaa aatggctgtc aatgaatggg
    30481 tttatgatgc cacctatcaa gcatggtatt atttgacatc agatggttct tatgcttaca
    30541 gtacatggca aggaaattac tatctaaaat cggatggtaa aatggctgtc aatgaatggg
    30601 ttgatggtgg acgttattat gttggcgctg acggagtttg gaaggaaggt caagcaagta
    30661 cagcttcttc tagtaatgat agcaatagtg aatattctgc tgctttagga aaggcaaaaa
    30721 gttataattc gttattccac atgtcaaaaa aaacgtatgt atagacaatt aacttctgat
    30781 tttgataaat tttcaaatga tgcagctcaa tatgccattg atcattagat gattaacaga
    30841 tactggtagg cgaaaaaatt ccttataatt catttacgca aacctttgca taagtgaatg
    30901 gattttgata tactagtact gtaagcattt tcaattacta cacaatgaaa aaggagaata
    30961 tgatgagtca aaagattatt gggattgacc ttggtggaac ttctatcaaa tttgcaatct
    31021 taacaacagc aggagaaatc caaggaaaat ggtcaatcaa gaccaacatt ttggatgagg
    31081 gaagtcatat cgttgatgat atgattgagt ctattcaaca tcgtttggac ttgcttggat
    31141 tggcagcagc ggacttccaa ggcattggaa tgggatcacc aggtgtggtt gaccgtgaca
    31201 aagggactgt tatcggtgcc tacaacttga actggaaaac ccttcaacca atcaaacaaa
    31261 agattgaaaa agctttgggc attccatttt tcatcgataa tgatgccaac gtagcagctc
    31321 ttggtgagcg ctggatgggt gctggagata accaaccaga cgttgtcttt atgacactcg
    31381 gtactggtgt tggtggcggt atcgtcgcag aaggcaaatt gcttcacggt gttgctggtg
    31441 cagcaggtga gcttggtcac atcactgttg actttgacca gccaatctca tgtacttgcg
    31501 gtaagaaagg ctgccttgag acagttgctt cagcaacagg gattgtcaac ttgactcgtc
    31561 gctatgccga tgaatacgaa ggcgatgcag ccttgaaacg cttgattgat aatggagaag
    31621 aagtaactgc taagactgtc tttgatctcg caaaagaagg agacgacctt gctttgattg
    31681 tttaccgtaa cttctcacgt tacttgggaa tcgcttgtgc taacattggt tcaatcctaa
    31741 acccatcaac aatcgtcatc ggtggtggtg tgtcagctgc gggagaattc cttctacaag
    31801 gtgttcaaaa agtttacgat gaaaatagtt tcccacaagt acgcacatct actaaattag
    31861 ctcttgcaac tctaggaaat gacgctggag ttatcggagc agcatcactt gtattgcaat
    31921 aagataatga gagaggaaaa tgctaaccta gagttagtaa tgttcctctt ttatttttta
    31981 tgctatacta gttaggacaa tgaatgaggt gagagtaaat gacaaaagca gatacgattt
    32041 ttaaagagaa tattgaatgc attctaaagg aaggtgtttt ctcagagcaa gctcgtccta
    32101 agtacaagga tgggactgtt gctaattcca agtatgtaac tggtgccttt tccgagtacg
    32161 acttatctaa aggagaattt cctatcacaa ccttgcgtcc cattgcaatc aaatccgcca
    32221 tcaaagaagt tctctggatt tatcaagacc agtcaaatag cttagaagtg cttaatgaca
    32281 agtacaatgt tcactactgg aatgactggg aagttggaga cacgggaacc attggtgagc
    32341 gctatggtgc cgttgttaag aaacacgaca ttatcaataa gcttctcaaa cagttggaaa
    32401 ccaatccttg gaatcgccgc aatattattt cgctctggga ttaccaagct ttcgaagaaa
    32461 cagatgggct gctcccgtgc gcctttcaga ccatgtttga tgtccggcgt gttgatgggg
    32521 aaatctatct ggatgcgacc ttgacccagc gctccaatga tatgctggtg gcccaccaca
    32581 tcaacgctat gcagtatgtg gctttgcaga tgatgattgc caaacatttt ggctggaagg
    32641 ttgggaagtt cttctatttc atcaacaacc tccatatcta tgataatcaa tttgaacaag
    32701 ctcaggaatt gctccgtcgg gagccgtcaa actgccaacc acgcttggtt ttaaatgttc
    32761 ctgatgggac taatttcttt gatatcaaag cagaagattt tgagttggtg gattatgacc
    32821 ctgttaagcc acagttgaag tttgacctag ctatttaaaa gaatagaaaa aagaagttga
    32881 gaataatccc aacttctttt gtttcttaac gtgatacgcg gcgacgagct gcttttttac
    32941 gattttcttc gatgaaagct gctttttgct cttctggttc gattactttc tttttaaatg
    33001 cgtatactgc acctgcaacg gcagcgacag ttcctgcgac acctgttaca agacctttag
    33061 cgaatccttt agccatgagt cttcctcctt tatattctca atcagccagc ctcctcaaga
    33121 ggtcacattt ttctgactga cctttttgtg ttataataat agtaacgaaa aaatgggaat
    33181 ttttcaagga aaaaagatga aaacaaaaat aattgtgatt gttggaccga ctgctgttgg
    33241 aaagacggct ctagctattg aagttgcaaa gcgctttaat ggcgaagtgg ttagtggaga
    33301 tagccagcaa gtctatcgag gacttgatat tgggacggcc aaggctagtc cagaagagca
    33361 ggcagctgtt cctcatcatt taatcgatgt tagagagata accgagtctt actcggcttt
    33421 tgattttgtt tcagaagcta agatgactat tgaggatatt cacagccgtg gcaagctagc
    33481 cattatcgcc ggtgggactg gactttatat ccagagcttg ctagaaggtt accacctagg
    33541 tggggagact cctcatgagg agattttagc ttatcgagct agtttggagc catattcaga
    33601 tgaggaatta gcccatttgg tggagcaagc aggccttgag attccccagt ttaatcgtcg
    33661 tcgtgctatg cgtgccttag aaattgccca ttttggtcag gatttggaaa atcaagagat
    33721 cttgtatgaa ccgctgatta tctgcttgga tgatgaacgt agtcaacttt atgagcgtat
    33781 caaccaccga gtggatttga tgtttgaggc tgggcttttg gatgaggcca agtggctgtt
    33841 tgaccattcc cctaatgtac aggctgctaa aggaattggc tataaggaac tctttcctta
    33901 tttccgtgga gagcagacct ttgaggaagc tcgtgagagt cttaaacagg cgacccgtcg
    33961 ttttgccaag cgtcagctga cctggttccg taatcgcatg caggtcacct tttatcagat
    34021 tggagaatct ggtgtacaag accgcatttt aagccagata gaggagtttt tagatgattg
    34081 aaacggagaa aaaagaggag cgagtcctgc tgattggtgt ggaattgcag ggtatggaca
    34141 gttttgacct ctccatggaa gaattggcta gtttagcgaa aacggcaggg gcagtcgttg
    34201 tagatagcta cagacaaaaa cgtgaaaaat atgattccaa gaccttcgtc ggctctggta
    34261 agttggaaga gattgcgctt atggtggatg cagaagaaat cactactgtc atcgtcaaca
    34321 accgtctgac cccaaggcag aatgtcaatc tagaggaagt tctcggtgtt aaggtcattg
    34381 accgtatgca gttgattttg gatatctttg ccatgcgggc tcgaagccat gaagggaagc
    34441 tccaagtcca cctagcccaa ctcaaatacc tcttgcctcg cttggttggt caggggatta
    34501 tgctcagccg tcaggcaggg ggaattggtt cccgtggtcc tggtgaaagc cagctggagc
    34561 tgaaccgtcg tagcgttcgc aatcaaatca cggatatcga gcgccagctc aaggtggttg
    34621 agaaaaatcg ggcgactgtc agagaaaaac gtttggagtc tagcaccttt aagattggtt
    34681 tgattggtta taccaatgct gggaaatcaa ctatcatgaa catcttgacc agtaagaccc
    34741 agtatgaagc agatgagctc tttgcgactc tggatgcgac aaccaagagt attcatctgg
    34801 gaggcaatct ccaagtaact ttgacagata ccgttggctt tattcaagat ttgccgacag
    34861 agttggtgtc cagtttcaag tcaaccttgg aagaaagcaa gcatgtggac cttctggttc
    34921 atgttatcga tgctagcaat ccttaccacg aggagcatga aaaaacggtt ctctccatca
    34981 tgaaagacct ggacatggaa gatattcctc acttgacgct ttataataaa gcggatttgg
    35041 tggaggattt cacgcctacc caaacgccat ataccctcat ttctgccaag tctgaggaca
    35101 gtcgtgaaaa cttgcaagca ttattgctag ataagattaa ggaaattttt gaagcattta
    35161 ccctgcgagt gcctttttca aagtcctaca agattcatga tttagagagt gttgcaattc
    35221 tggaagaacg tgattatcag gaagacggcg aagtgattac aggctacatt tccgagaaaa
    35281 ataaatggag gttagaagaa ttttatgact gatattaaaa ctttggctct aaagtatgga
    35341 ggttatacaa gtctggacaa ggtctatctg gatcagcttc tagctggtaa aacagagcag
    35401 gagcagttaa cacttattac acctccgcca agtgttgtta atgcctactt tgcagaactc
    35461 tatcagaaaa aaagtcctca ggctgcgacg gattattttg cagaactcag tcaggaactg
    35521 aatctttaca atactgagcc aagtttcacc ctagaaaata agccttttat tcggcttaat
    35581 ctgtctggta aatcctttgg tttttgttat gagagtgacg gctttggtcg gattttctct
    35641 gaaaatgagg agaaaatctc ggatgacttg ctctttgaaa ttgcgcaaat tttctcccat
    35701 caattagtct ttgaagagtc tggtaagatt tacatgaagc ctgtcaagga cgaggaagtt
    35761 gtcagcctag aaagtctcac agctttgact gatttggaaa gattggctga tggtcgaaaa
    35821 cgtctcaaag gctacagcca agaggattta ttgcaagaag ctgctgcttt ttctggcaaa
    35881 cgctatttcc gatcggaaaa ccgcacagcc atgttatata ttgattaatt agaaagtatc
    35941 gaatggatat tcaattttta ggaacggggg ctggtcagcc ctctaaagcc cgcaacgttt
    36001 caagtctcgc cctgaaactt ttggacgaga ttaacgaagt ttggctcttt gactgtggag
    36061 aaggtacgca aaatcgcatt ctggaaacca caattcgacc acgtaaggtc agcaaaatct
    36121 ttattaccca tctgcatgga gaccacattt ttggtttgcc aggtttcctt tctagccgtg
    36181 cctttcaggc caatgaagag cagacagatt tggaaatcta cggacctcaa ggaatcaagt
    36241 catttgtctt aaccagcctt cgtgtgtcag gttctcgtct gccctaccgc attcatttcc
    36301 atgagtttga ccaagattct ctgggtaaaa ttcttgaaac cgataaattc actgtgtatg
    36361 cagaggagct ggaccacact attttctgtg ttggctatcg tgtcatgcaa aaggatctag
    36421 aagggacgct ggatgctgaa aaactcaagg ctgctggtgt tccgttcggc ccgctttttg
    36481 gtaaaatcaa aaacggccag gatcttgttt tggaagacgg aactgaaatc aaggcagcag
    36541 actatatctc agcgccacgt ccaggtaaga ttatcactat tttaggagac actcgaaaaa
    36601 cggatgccag tgtgcgtctg gctgtcaatg cagatgtcct agttcatgag tccacttatg
    36661 gcaagggtga tgaaaaaatt gctcgtaacc atggtcactc aactaatatg caagctgcac
    36721 aagtagcggt agaagcaggt gccaaacgcc tcctactcaa ccatatcagt gcccgtttcc
    36781 tctcaaaaga tataagcaaa ctcaagaagg acgctgccac aatttttgaa aatgtccatg
    36841 tggtcaaaga cttggaagaa gtggaaatct agcagtcaca gaaaggataa gtatgcctac
    36901 tattctcatt accggagcta gcggtggtct agctcaagaa atggtcaaac tcttgcccaa
    36961 tgaccaactc atcttgcttg gtagaaataa ggaaaaatta gcccaactct acggaaatta
    37021 ttcccatgca gaattgattg aaattgatat taccgacgat tcagccctag aagctctggt
    37081 aactgatctt tatctccgct atggcaagat tgatgtcttg attaacaacg ctggttacgg
    37141 gatttttgag ggatttgacc agattgctga taaagatatt caccagatgt ttgaggtcaa
    37201 tacctttgcc ctgatgaatc tgtctcgtca ccttgcggct cgtatgaagg aaagcagcaa
    37261 agggcatatc atcaacatcg tcagcatggc aggtctaata gctactggca agtctagtct
    37321 ttactcagcg accaagtttg cggctattgg tttttcaaat gctctgcgac tcgaacttat
    37381 gccctatgga gtctatgtga caacagtcaa tccaggtcca atccgaacag gattttttga
    37441 ccaagctgac ccagatggaa cttatcttaa atcggttgac cgcttcctct tagaggcaga
    37501 tgcagtggct aaaaagattg tcaagattat aggcaaaaat aaacgagaac tcaatctccc
    37561 gattttgttg aacctagccc ataagtttta tactctcttt cccaagctag ctgataagtt
    37621 ggcaggggaa acttttaatt ataagtaaaa agaaccaatg tgcaggttgt tgctagccta
    37681 catattggtt cttatcattt ctcaactatc aaactgaact tcttctagta gatagtcgat
    37741 aaagcgttcg cccatcttag ataggctggt tttctcatgc tggatataga ccagctcaat
    37801 cgggtcatca atatccagtg ggatagaaac gatattgtct ccgtttaggt tgctgttcaa
    37861 aatccctgtc gcaatggtat aaccatccaa accaatcaag agattaaaga gggtggcacg
    37921 gtcactgacc acaatggatt tcttgtggtg ttcctgagaa agaatctctt ctgaaaagta
    37981 gaaggagttg tgcgtccctt ggtcatagct gaggtaaggg aaattctcca aatcagacag
    38041 tttcaccttg tctttctttg ccagagggtt ggtcttgctg acaaagatat gcggttgcgc
    38101 tgtgaagaga tggtgggcta gcaggtgatt gtcatccagc atcttggtta aaacatcacg
    38161 gttgtaactg tttaagaaga ggaccccgac ctcactgcgg aagttcttga cgtcgtcgat
    38221 aatctcccaa gtccgagttt cacgaaggaa gagttcgtat ttctccatat cgcttttctt
    38281 gagcaaagag acaaaggcat tgaccacaaa ggcatagtgt tgagacgaaa cgctaaagag
    38341 ttcgcggtgg gcgacaggat ttttatagcg ttcctccaga agctgggtct gctcgacaac
    38401 ctgacgggca taagagagaa actccatccc atcacgggtc aaggtgattc ccttgggatt
    38461 gcggataaag atctcaatgc ccatttcatt ttccaaatct cgcactgcat tggagagact
    38521 tggctgagtg ataaagagtt gcttggctgc ctcattcatg gagccagttt cgacgatttt
    38581 gataatataa tgtaattgtt gaattctcat gtttttattg taccatactt gccttagaat
    38641 cgctaaagaa gataggaaaa taaggagcat tgtatgagtc tcttcttgaa aaaaacacaa
    38701 aattttgcta gaatgagtct tatgaaaaca ttctatgatg tgcagcaatt cctcaaacga
    38761 tttggtatta ttgtttacat gggaaaacgc ttatatgata ttgaactgat gaagttggaa
    38821 ctatctcgga tttacgatgc agggttgatg gataaattag actatctaga ggcagaagca
    38881 gttcttcgca gagagcacaa ggtagaattg gattatattg agaaaaatgg agaaaagaac
    38941 taatggttac ttggattttg tgggcactta tactagcaat gttggcgtgg atgggcttta
    39001 actatcttcg tattcgccgt gcggctaaaa ttgtggacaa taaggagttt gaagccttga
    39061 ttcgtacggg tcaattgatt gatttgcgcg acccagcaga attccacaga aaacatatcc
    39121 ttggtgcacg caatattcct tcaagtcagt tgaaaactag tcttgcagca cttcgtaaag
    39181 ataaacctgt ccttctctac gaaaaccaac gtgcgcaacg agttacaaat gcagctcttt
    39241 acttgaaaaa acaaggtttt tctgagattt atatcctttc ttatggcttg gattcttgga
    39301 aagggaaagt gaagactagc taaccttatt tccaaattag tcgaatgata ggatgaagct
    39361 attgttttat agtataatta atttgtataa aatttaaaga gttcttagaa catgaaagaa
    39421 aagaagaaaa atccactttt tgtgggtatc ttgtcgatta ttcttggttt gttatttcca
    39481 attgtaggcc tgattttggg tatcatagga ttggtcttgg ctatttcata ccaaaaagag
    39541 tcacaattag actataaaat agaaaagatt cttaatatct taggaattgt gatttctgta
    39601 gttaactgga ttgtagcaat cgccttaatt tttcggtaaa taaaagaaaa aagctttagt
    39661 tttaaagctt tttttatttt atcgactcat ctctaaatag tttttaatga tttgtaactc
    39721 tttttggttc aaagggcggt agttaccttc tgctaaatct gggttcaatg tgaagtcccc
    39781 aaattggatt cttttgaggc tagtcacctt aacaccaacc gagaggaaca ttttcttgat
    39841 ttgatgaaat tttccttctg aaatggtgat agaggcttga ctgagggagg gacttgctga
    39901 tagaatctct agtcttgcag gtttacagat agtgccatct aaaaagacaa ttcccttttg
    39961 aaaggcttgg atatggtcag gtgttagaag tccattaacc tcaacttgat aagtcttatc
    40021 gacatgatat tggggatgga ggagttggaa gcccaaggga ccgttatcgg tcaagagaag
    40081 gagtcctgtt gtatctcggt ccagtcggcc gacggcatag agcttgtcag actgaatgtc
    40141 agatggaagc aggtccatga cggtcggaag ttccttgtct ttgttggctg taacgacacc
    40201 agcaggttta tgaagcataa gataggtgtg ttcatagcct tgaatgattc gatcctgaaa
    40261 aaggagttct tgtagtcctg tatcgatatt ttgggctagg gagcgggctg ggcaactatc
    40321 gacgagaatg tcccctttga gtaaggcttg tttcatggcc tttcggctga ttttttcttg
    40381 agctaataaa ttatctaaac gcatactgtg cctatcttat cataagtcgc ttgctttgaa
    40441 aaggcttgac gtgaaaagaa aagcggagtg aaaacattta cacttgcttg agttatgtta
    40501 tttatttgaa attatggtat aatcgttcag ttagaaaata aattttgaat attatagagg
    40561 aaatcatgac aaaattaaga gaagatatcc gtaacattgc gattatcgcc cacgttgacc
    40621 acggtaaaac aaccctggtt gacgaattat tgaaacaatc agaaacgctt gatgcacgta
    40681 ctgaattggc agagcgtgct atggactcaa acgatatcga aaaagagcgt gggattacca
    40741 tccttgctaa aaatactgcc gttgcttaca acggaactcg tatcaacatt atggacacac
    40801 caggacacgc ggactttggt ggagaagttg agcgtatcat gaaaatggtt gacggtgttg
    40861 tcttggtcgt agatgcctat gaaggaacca tgccacaaac tcgtttcgta ttgaaaaaag
    40921 ccttggaaca agaccttgtc ccaatcgtgg ttgttaacaa aatcgataag ccatcagctc
    40981 gtccagcaga agtagtggat gaagtcttgg aacttttcat cgagcttggt gcagatgacg
    41041 accagcttga tttcccagtg gtttatgctt cagcgatcaa cggaacttct tcattgtcag
    41101 atgatccagc tgaccaagaa gcgactatgg caccaatctt tgacacgatt atcgaccata
    41161 tcccagctcc agtagataac tcagatgagc ctttgcagtt ccaagtgtca cttttggact
    41221 acaatgactt cgttggacgt atcggtatcg gtcgtgtctt ccgtggtaca gttaaggttg
    41281 gggaccaagt taccctttct aaacttgacg gtacaactaa aaacttccgt gttacaaaac
    41341 tcttcggttt ctttggtttg gaacgtcgtg aaatccaaga agccaaagcg ggtgacttga
    41401 ttgccgtttc aggtatggaa gacatctttg tcggtgaaac catcactccg acagatgcag
    41461 tagaagctct tccaatccta cacatcgatg agccaactct tcaaatgact ttcttggtca
    41521 acaactcacc atttgctggt aaagaaggta aatgggtaac ttctcgtaag gtggaagaac
    41581 gcttgcaggc agaattgcaa acagacgttt cccttcgtgt tgacccaact gattcaccag
    41641 ataaatggac tgtttcagga cgtggagaat tgcacttgtc aatccttatc gaaacaatgc
    41701 gtcgtgaggg ctatgaactt caagtatctc gtccagaagt tatcgtaaaa gaaatcgacg
    41761 gtgttaaatg tgaaccattt gaacgtgtac aaatcgacac tccagaagaa taccaagggt
    41821 ctgttatcca aagcctttct gaacgtaaag gtgaaatgtt ggatatgatt tcaactggta
    41881 atggtcaaac tcgtttggtc ttccttgttc cagcgcgtgg tttgattgga tactcaactg
    41941 agttcttgtc aatgactcgt ggttacggta tcatgaacca taccttcgac cagtacttgc
    42001 cattgattcc aggggaaatt ggtggacgtc accgtggtgc ccttgtttct atcgatgctg
    42061 gtaaggctac aacttactca atcatgtcta tcgaagaacg tggtacgatc tttgtcaacc
    42121 caggtactga ggtttatgaa ggaatgatca tcggtgaaaa ctctcgtgaa aatgacttga
    42181 cagttaacat cacgaaggca aaacaaatga ccaacgttcg ttcagctact aaggaccaaa
    42241 cagctgttat caagacgcct cgtatcttga cacttgaaga gtctcttgag ttcttgaacg
    42301 acgatgagta catggaagta acgcctgagt ctatccgttt gcgtaaacaa atccttaaca
    42361 aagcagagcg tgagaaagct aacaagaaga aaaaatcagc tgaataagag ctagaaagag
    42421 agaaagatgg tctatttagt cctaggaatt ttactgctcc tactctatgt atttgcgaca
    42481 ccagaaagca ttaaagggac agtcaatatc gtcgctatgg tatgtatttt agtggcactc
    42541 ttgattttat tggttctatc ttttctgaaa atttttcaat taccaacaga aatattccta
    42601 gcaatagcca tgttgatcct agcttacttt agtgtgagag acatcacact catgccagtc
    42661 aaaaaaagta aaagaagata aaaaagagag tttcggctct cttttttgct aggaatttta
    42721 caagattgca atttattttt gagttatcac catctctttt gtttggataa tatgaatggg
    42781 aaaaacttga ataaaattct ttaatggcat ctattttata tttggttagt actggttgca
    42841 tattattttg a
//
LOCUS       DAVKAO010000010        41454 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKAO010000010 DAVKAO010000000
VERSION     DAVKAO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.010"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..320
                     /locus_tag="VWX16_000565"
     CDS             <1..320
                     /locus_tag="VWX16_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9157959.1"
                     /translation="KHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSQDNG
                     MMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTK
                     SFG"
     gene            complement(447..650)
                     /locus_tag="VWX16_000566"
     CDS             complement(447..650)
                     /locus_tag="VWX16_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9157960.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(643..1725)
                     /locus_tag="VWX16_000567"
     CDS             complement(643..1725)
                     /locus_tag="VWX16_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9157961.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1851..2633)
                     /locus_tag="VWX16_000568"
     CDS             complement(1851..2633)
                     /locus_tag="VWX16_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9157962.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGTHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2633..3976)
                     /locus_tag="VWX16_000569"
     CDS             complement(2633..3976)
                     /locus_tag="VWX16_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9157963.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4075..5391)
                     /locus_tag="VWX16_000570"
     CDS             complement(4075..5391)
                     /locus_tag="VWX16_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9157964.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYLEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5585..6811
                     /locus_tag="VWX16_000571"
     CDS             5585..6811
                     /locus_tag="VWX16_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9157965.1"
                     /translation="MKSNRYIIAFTGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYILWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7010..8584
                     /locus_tag="VWX16_000572"
     CDS             7010..8584
                     /locus_tag="VWX16_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9157966.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8862..9650
                     /gene="codY"
                     /locus_tag="VWX16_000573"
     CDS             8862..9650
                     /gene="codY"
                     /locus_tag="VWX16_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9157967.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9650..10225
                     /locus_tag="VWX16_000574"
     CDS             9650..10225
                     /locus_tag="VWX16_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9157968.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10307..10392
                     /locus_tag="VWX16_000575"
     tRNA            10307..10392
                     /locus_tag="VWX16_000575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10341..10343,aa:Leu,seq:aag)
     gene            10676..11806
                     /gene="ugpC"
                     /locus_tag="VWX16_000576"
     CDS             10676..11806
                     /gene="ugpC"
                     /locus_tag="VWX16_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9157969.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(11953..12099)
                     /locus_tag="VWX16_000577"
     CDS             complement(11953..12099)
                     /locus_tag="VWX16_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157970.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            12307..13065
                     /locus_tag="VWX16_000578"
     CDS             12307..13065
                     /locus_tag="VWX16_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9157971.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            13152..13664
                     /locus_tag="VWX16_000579"
     CDS             13152..13664
                     /locus_tag="VWX16_000579"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9157972.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13846..14790
                     /locus_tag="VWX16_000580"
     CDS             13846..14790
                     /locus_tag="VWX16_000580"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9157973.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWIKIEDDASAYGYL"
     gene            14799..15476
                     /locus_tag="VWX16_000581"
     CDS             14799..15476
                     /locus_tag="VWX16_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9157974.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15573..16331
                     /locus_tag="VWX16_000582"
     CDS             15573..16331
                     /locus_tag="VWX16_000582"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9157975.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNFKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16369..17691
                     /locus_tag="VWX16_000583"
                     /pseudo
     CDS             16369..17691
                     /locus_tag="VWX16_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-beta-N-acetylmuramidase"
     gene            18188..18706
                     /locus_tag="VWX16_000584"
     CDS             18188..18706
                     /locus_tag="VWX16_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9157976.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            18836..19342
                     /locus_tag="VWX16_000585"
     CDS             18836..19342
                     /locus_tag="VWX16_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9157977.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQVQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPKELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19342..19512
                     /locus_tag="VWX16_000586"
     CDS             19342..19512
                     /locus_tag="VWX16_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157978.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            19544..20776
                     /gene="clpX"
                     /locus_tag="VWX16_000587"
     CDS             19544..20776
                     /gene="clpX"
                     /locus_tag="VWX16_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9157979.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20785..21372
                     /locus_tag="VWX16_000588"
     CDS             20785..21372
                     /locus_tag="VWX16_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9157980.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21383..21763
                     /locus_tag="VWX16_000589"
     CDS             21383..21763
                     /locus_tag="VWX16_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9157981.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21815..22705
                     /gene="rapZ"
                     /locus_tag="VWX16_000590"
     CDS             21815..22705
                     /gene="rapZ"
                     /locus_tag="VWX16_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9157982.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22702..23679
                     /locus_tag="VWX16_000591"
     CDS             22702..23679
                     /locus_tag="VWX16_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9157983.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNYDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23676..24587
                     /gene="whiA"
                     /locus_tag="VWX16_000592"
     CDS             23676..24587
                     /gene="whiA"
                     /locus_tag="VWX16_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9157984.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24621..25586)
                     /locus_tag="VWX16_000593"
     CDS             complement(24621..25586)
                     /locus_tag="VWX16_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9157985.1"
                     /translation="MSQLYDITIVGGGPIGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25722..26579
                     /locus_tag="VWX16_000594"
     CDS             25722..26579
                     /locus_tag="VWX16_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9157986.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     ANISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26566..27345
                     /locus_tag="VWX16_000595"
     CDS             26566..27345
                     /locus_tag="VWX16_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9157987.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27369..28721
                     /locus_tag="VWX16_000596"
     CDS             27369..28721
                     /locus_tag="VWX16_000596"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9157988.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEVPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATSAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28795..29169
                     /locus_tag="VWX16_000597"
     CDS             28795..29169
                     /locus_tag="VWX16_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9157989.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29171..30019
                     /locus_tag="VWX16_000598"
     CDS             29171..30019
                     /locus_tag="VWX16_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9157990.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30285..31052
                     /locus_tag="VWX16_000599"
     CDS             30285..31052
                     /locus_tag="VWX16_000599"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9157991.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLFMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31064..32248
                     /locus_tag="VWX16_000600"
     CDS             31064..32248
                     /locus_tag="VWX16_000600"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9157992.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32245..34116
                     /locus_tag="VWX16_000601"
     CDS             32245..34116
                     /locus_tag="VWX16_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9157993.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLNKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMSRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34259..35443)
                     /gene="mntE"
                     /locus_tag="VWX16_000602"
     CDS             complement(34259..35443)
                     /gene="mntE"
                     /locus_tag="VWX16_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9157994.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLATGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRLARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPILEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35747..38443
                     /locus_tag="VWX16_000603"
     CDS             35747..38443
                     /locus_tag="VWX16_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9157995.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQTMDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVFRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38456..39247
                     /gene="yghU"
                     /locus_tag="VWX16_000604"
     CDS             38456..39247
                     /gene="yghU"
                     /locus_tag="VWX16_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9157996.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39363..39773
                     /locus_tag="VWX16_000605"
     CDS             39363..39773
                     /locus_tag="VWX16_000605"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9157997.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39785..41380
                     /locus_tag="VWX16_000606"
     CDS             39785..41380
                     /locus_tag="VWX16_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9157998.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        12167 a         7782 c         9461 g        12044 t
ORIGIN      
        1 agaagcacta tgagaatacg attctccata gtgaccaagg ctggcaatat caacacgatt
       61 cttatcatcg gttcctagag agtaagggaa ttcaagcatc catgtcacgt aagggtaaca
      121 gccaagataa cggtatgatg gaatctttct ttgggattct gaaatcggaa atgttttacg
      181 gttatgagaa gtcgtttcag tcgcttaagc aattggaaca agctattgta gactatattg
      241 attactacaa caataaacga attaaggtaa aactaaaagg acttagccct gtgcaataca
      301 gaactaaatc cttcggataa attaattgtc taactttttg gggtcagtac agaaaaactt
      361 tgatactatg cgttttattg tgggaagatt ttcctcattt tctagtgaaa ttgaactttt
      421 tctaacatct tttgatagat gacttcttaa tcaattatca tttccattgt tgtttcttgg
      481 ggtttcatcc cttgtctttg ataaaagcgg agagcaccct cgttatcatt ccagacactt
      541 agggttagat tattgcagcc aatttcttta gcataatagc agaaaaatga caataaaata
      601 gcccctagat attttgaaag gaaagcatag gggctatata gtttaatcaa aataaagcaa
      661 atctttgctg gtgcttgtaa agaggtcgaa gccatccttg gtaacaacac cgcagtcttc
      721 aatacgaaca ccgactttac cagggatata gatacctggt tcaacagaga agcacatgcc
      781 ttcttcgatg accatgtcgt ttccttccat gatagatggg aattcatgga catccatacc
      841 gataccatgc ccgagacggt ggttgaagta ctcaccataa ccagcttttt cgatgacctc
      901 acgggcagcg cggtccactt catgagcagt cacacctggc ttgataaagt caagagcagc
      961 ttgttgggct tcaagagtca agttgtaaat atctttcttg aattggtctg gtttgccgac
     1021 agcgactgta cgagtcatat ctgacgcata gccattgacc agaacaccca ggtcaaagag
     1081 gagaagagca tcattttcaa ccttattagc tgctggaatg ccgtgtggat tcgcagcatt
     1141 atcaccagtc aagaccatgg tatcaaagct catttcgtaa ccttcacgtt tcatggcaaa
     1201 gtcaatttgt gcgatgatat ctgtctcagt cttatcaaga gaaatattgt caaaaccaac
     1261 atgaacagcc ttgtcagcat aaagacctgc aaccatcatt ttttgtactt catcagctga
     1321 tttgatgagg cgcatgcgtt ggatacgagg agtgaggttt tcaaactcag cagtttcaaa
     1381 aactgttttc aaaccatggt atttggtcaa gatgagattg tcaaactcaa cagcgacacg
     1441 tttgaagtca agttgtggaa gagcatgttt gattttttgc catggatttt cagaatcgac
     1501 atagcccact actgggaagg aaacggtgct acttgcacgt tctacttcaa gagctgggac
     1561 aaagaggaga ggttcctgat ccgctaggac aaagaggaac atttggcgtt catggggatc
     1621 actgtaaaaa ccagtgaggt aattgattgt gacggggtca gatacgacag cgacgtctag
     1681 tttttctgat tcaagatatg ttaggatttg ttgtaattta gacatatgct accttctttc
     1741 tacccctcta ttttggcaaa aaatgagaga aaatgcaagg gagaagggta aaagaaaaga
     1801 caaaaaactc cgacaagata acggagttct ttgatatcat tttcctttgt ttaagaaaag
     1861 tcagccttgc ttttgacgtc actgtactct tcagcgattt cttggctgag aatgtcctca
     1921 taggcaggga gttggatgtc ctgaccatat ttcttcttga gggcagtagt aactaggcga
     1981 taagccagat tggcattacg agagaggata ggcccgtgga agtaggaacc aaagacattc
     2041 ttataatgaa ccccttcacc gaccttttct tcgttgtttc catttccata gacaacctgt
     2101 cccagcggtt tttggtcatc agagaggaag gtacgacctt ggtgattttc aaatccatag
     2161 taggtttcat cgaaatcttc attgtgaatc ttgatgtcac cgataaaacg gttattggtc
     2221 tggttgagcg tgtagtgtcc catgacccct agcccttcga tacgttttcc tgaagcttca
     2281 acataatatt gacccaatag ttggaaacca ccgcagatag ccagaactac accgtcgttt
     2341 tggatgtagt tgtcaatgct ctctttttta gcaggtaggt cgtctgcaat gatactttgt
     2401 tcaaagtctt gaccaccacc gaaaaaggcg atgtcgtagt gattttcatc aaagtcatca
     2461 tggagagaaa cgatgtcaac ggtcacatgg gttcccagtt tttcagccac atacttgagc
     2521 atgaggatgt ttccattgtc cccgtaggta ttcatgagat ttccgtagag gtgggcaatg
     2581 ttgagctgat aggggtaatt gccatctttt gaggaaagtg aagtataaac cattagttca
     2641 tctcctttct aacaatctga cgactagcca gcagttcacg aaattccagc atggcagtat
     2701 aagttgccag aatataggca tgcttgcagt cttgattctc aatggtcttg agaacttgct
     2761 ccagattact cgtttcagtg attttctcag ctggatagcc agtcactcgg aggcgacgag
     2821 cgatttcaga atgacgaaca ccgccagcgt tgatttcagg aatgtccatg tcagtgattt
     2881 gctcaaagtc tgcatcccag atccagctag tgtcaattcc atctgcatag ttggcattaa
     2941 ggaggacaga taggctaaat ggataaggtg ctagtttgat catttcgata gcttgggttg
     3001 caccgactgg atttttaatc aagacaaggg tacattcctt gtcaccgata tgaaaggttt
     3061 cttggcgtcc aaagacagca cggctcttgt caaatccctg tttgatgagt tgcgaatcgg
     3121 cacctaggaa acgggcgatg gccacagcag ctagggcgtt atagatatta tagagcccgc
     3181 cgatttggat accgtattct tggccgtcta tgacaaagcg agagcgattg ttggtcaact
     3241 caaccagttt tgtcaaacga tagtcgagat caggacgttt acatccacaa ccctcacaga
     3301 tataggcacc caagtttgca taggtattat gctcatattt gaggatgcct tggcagtcag
     3361 gacagagaat cccttcggta ttgtagtgag ccagttgggc tggtcctttt tccaagtcaa
     3421 aaccaaaata ctctataggg tttggaatag ttggcttgta gaaaagtgga ctgtctccgt
     3481 taaggagaac agtagcagtt ggaactttcc gaatggcatc caatatcatg ttataggtag
     3541 tatagatttc accgaaacgg tccatctggt cacggaagat attagtaatg acaaaaagac
     3601 taggctggat atagtcacag atacgagata gactggcttc gtcaatttcg aggacggcaa
     3661 tatttttccc agttttagaa gatttggctg ttaggaaggt tgttgcaatc cctgtaatca
     3721 tgttggcacc gcttgggttg gttagaactt gaccataaac ctcttttaaa atgccgacag
     3781 tgagggcagt tgtcagggtt tttccatttg ttccagtgac aacgacaatc tcgtagttct
     3841 tagctaggtt ttgtaaaata tctttatcaa attgaagggc gactttccct gggagcgtac
     3901 ttccacgtcc aagacggctt aaaacgaagt gggaagaacg cccagcaaga aggcccaaag
     3961 tagtttttaa gttcatgttt ctattatatc acaaaagagg gaaaagacag atttggtgtt
     4021 tcgtagaagt aaaaacagcc caaagagggc tgtcagttaa gatgagattt caacttaaat
     4081 cgcaaacaag tcattcaagt tctcagccaa ggttgggtga gtgaagattt gttttgtgaa
     4141 gtaagtgtaa ggaatcttgt tgtccatagc aacagtaatg atgttgatga tttcttgaga
     4201 accttctgag aagatgcttg caccaagaat ttcttttgtt tcagtattaa caacagcttt
     4261 gaaagctccg cgaaggtctc catttacgtg accacgaggc atggctgcaa cagggatttc
     4321 tttcactgcg tatggaagtt tcaaatcagc tgcttggctt tcagtcaaac caacttgtga
     4381 aagtgcaggt gtgatgaaca tagtatttgg tacattgaga cggtcctcaa gtgtgtagct
     4441 gccatctcca gcaaggtagc tgtaaacaac acggaagtca tcaagtgaaa tgtaagtaaa
     4501 ttgaagacca ccgttgacat ctccaactgc aaagacacca ggaacgtttg tttgacagtg
     4561 tttatctact ttaatagcgc cacgttcagt tagttcaata tctgtatttt caagttgaag
     4621 tggttctaca tttggtttgc gtccagttgc gtagagaagg gcgtcgaaac ggtaagtttc
     4681 gtcttcagtt acgacaagca cttggtcacc gtcgttttta atttcagtag tatggatatt
     4741 ttgaagcaat tcaataccgt cttcttccag gtattgttta gcaagagctg cgatggaagg
     4801 ttctgcacga ggtaggaatg tatccaaggc atctaggact gtaaccttgc ttcctagttt
     4861 attgtaaagg ccagcaaatt caagaccgat atttccgcca ccaaggactc caagtttttc
     4921 aggcaattta tccaagcttt ggatacctgt tgagtcaaag acgtttttgc ttgtagcaag
     4981 tccagggatt ggcaagacgt ttgaaacagc accagtgttg atgacgatag tttcagcagt
     5041 caattcttgt ttttcatcac cagcttggat ttcgatgact ttatttgaaa ggaagtgagc
     5101 ttcagcatca aagatatcga cgccagtacc agcaacagtc gtatagtttt taccgttgag
     5161 gcgaccagtg atcgtgtttt tggtagcaat gacttcttca aaagacaagt ctttctcagc
     5221 agcaaccagc aaagttttag ttgggataca accgatgttg atacaagttc caccgtacat
     5281 agctttgcta cgttcaacga gggcaacttt tttgccagct gaagccaatt tacctgctag
     5341 tgttttacca gctttaccaa atccgataac gattaaatca taagttaaca tttagagttc
     5401 ctcctattcg catttcattc tagcacaaga aaaaaacttt tgcacaattc tgctacggaa
     5461 aatacagaaa aagataaaaa cgctttccag acaaaaaact agtaattttt tgacaagctt
     5521 gataaactaa aatcctatct atttttatat aagatagaat aaatgttcga aaataggagg
     5581 ttttatgaaa tctaatcgtt atattattgc ctttactggg gtcattttac acttaatgct
     5641 gggttcgact tatgcttgga gtgtttatcg taaccctatt attgaaaaaa cgggatggga
     5701 tcaggcttct gttgcctttg cctttagtct agcaatcttt tgtttgggct tatcggctgc
     5761 atttatgggg cgtttggtag aaaaatttgg tccgagagtc atggggagtc tatctgcttt
     5821 tctatacgca ggtggaaata tcttaacagg atttgcaata gaccgtcagg aactgtggtt
     5881 gttgtatctc gcttatggca ttttaggtgg gcttggtttg ggagcaggct atattacccc
     5941 tgtgtcaacg attataaaat ggtttcctga taaacgtggt ctcgcaacag gtttagcgat
     6001 tatggggttt ggttttgctt ctttattgac tagtcccata gcgcaacatc tcatcgcagg
     6061 ggtagggctt gtagaaactt tttatatttt aggtgcaagt tactttatta tcatgctcct
     6121 agcttcacaa ttcattaagc gtccaaatga gcaagagctt gcaattttat ctgcttcagg
     6181 gaaagaaaaa acagactctc tgacgcaagg gatgactgca aatcaggctc taaaaagcaa
     6241 tcggttttat atactttgga ttattttctt tatcaacata gcttgtggtt taggcttaat
     6301 ttcagcggca tcgccaatgg cacaggagat ggctggcttg tctacaagtc atgcagcagt
     6361 aatggtgggc gttttaggga ttttcaatgg atttggtcgc ttgctctggg cgagcttatc
     6421 tgactatatc ggtcgccctc taacctttag tatattactg cttgttaatc ttttcttttc
     6481 tctctcactt tggctcttta cagattccgt tttatttgta gtcgctatgt ctattttgat
     6541 gacttgctat ggagctggtt tttcattgat cccagcttat ctcagtgata tttttggaac
     6601 caaggaattg gccgctctgc atggatatat tttaacagct tgggcaatgg ctggtttagc
     6661 gggacctatt ttattagcag agacttataa aatggctcat tcgtacacac aaaccttgtt
     6721 cgtttttctc attttataca gtatcgcctt ggctttgtct tattatctag gtcgttcaat
     6781 caaaaaagaa agtcaaaaac cgcttacatg attttctgta gaaatggggc tatcttttgc
     6841 tctggtcttg tgttatacta gataggttgc aaagaaaaca gtacttttct tttgtggaaa
     6901 agaagcaaca cgattttata tctagttata tgaaaatacg tcataaaaag aaaagtataa
     6961 actaagccct atagggtggc ttcgcaccac ctttagaaag aagaataacg tgaaatttaa
     7021 tgaattaaac ttgtctgctg atttgctagc agaaattgaa aaagctggtt ttgtagaagc
     7081 tagtcctatc caagaacaaa ctattccttt ggcccttgaa ggcaaggatg ttatcggtca
     7141 agctcagact ggtacaggaa aaactgcagc ctttggcttg cctacccttg aaaaaatccg
     7201 tacagaagaa gcgactatcc aagctttggt catcgctcca actcgtgaac tagctgttca
     7261 aagtcaagaa gaactcttcc gctttggtcg tagtaaggga gtcaaagtcc gttcagtata
     7321 tggcggatca agcattgaaa aacaaattaa ggctcttaaa tctggtgccc atatcgtggt
     7381 gggaactcca ggtcgcctct tggacttgat taaacgcaag gccttgaaat tacaagacat
     7441 tgaaaccctt atccttgacg aagcggatga aatgcttaac atgggcttcc ttgaagacat
     7501 cgaagccatt atttcccgtg tacctgagaa ccgtcaaact ttgcttttct cagcaactat
     7561 gccagatgcc atcaaacgta tcggtgttca gtttatgaaa gcccctgaac atgtcaagat
     7621 tgcggctaag gaattgacaa cagaattggt tgaccagtac tatatccgtg ttaaggaaca
     7681 agaaaaattt gacaccatga ctcgtctcat ggatgtggca caaccagaac tcgctattgt
     7741 atttggtcgt accaaacgcc gtgtggatga attgactcgt ggtttgaaaa ttcgtggctt
     7801 ccgtgcagaa ggaattcatg gcgacctaga ccaaaacaaa cgtcttcgtg tccttcgtga
     7861 ctttaaaaat ggcaatcttg atgttttggt tgcgacagac gttgcagcgc gtggtttgga
     7921 tatttcaggt gtgacccatg tctacaacta cgatattcca caagatcctg agagttatgt
     7981 tcaccgtatc ggtcgtacag gtcgtgctgg taagtcaggt caatctatta cttttgtttc
     8041 tccaaacgaa atgggttacc ttcaaatcat tgaaaacttg actaagaaac gcatgaaagg
     8101 tctcaaacct gcaagtgtag aagaatcctt ccaatcaaaa aaacaggtag ctctcaagaa
     8161 aatcgaacgt gattttgcag atgaaaccat tcgtgccaac tttgagaaat ttggtaagga
     8221 tgctcgcaaa ttggctgctg agtttactcc agaagaattg gcaatgtata tcttgagtct
     8281 gacagtccaa gatccagata gccttccaga agtggagatt gcacgtgaaa aaccactacc
     8341 gtttaaacca tcaggtaatg gtttcggtgg taaagctaag ggaggtcgtg gaggccgtcg
     8401 tggggacgac cgtcgagagc gtgatcgccg tggcaatggt cgccgtgatg agttcaaaaa
     8461 aggaagtcgt ggcaacgatc gttttgataa ggaaaaacgt taccgtaagg ataataaaaa
     8521 accacgcaat actttaagcg aaaagcaaac aggctttgtt attcgtaaca agggcgataa
     8581 ataagaaaaa gcggttcaaa atgaatcgct tttttatata aggagaccga atgtgaaaag
     8641 acaaataaaa ttatcatatt atcttttata atgttatgat acacaaatta tgatacacaa
     8701 aaataggcaa cttgtcaata gaaaaggaac aatttaatta aaaatattct ggtttttaat
     8761 taaattgtaa gtcaatgcaa gtcaataagg aattttcaga taattattga aaaagagacg
     8821 tttttatgtt ataatgatag cgattaaatt cgaggtgaaa aatggcacat ttattagaaa
     8881 aaactagaaa aattacatca attttgaaac gctcagagga gcagttgcag gatgagctcc
     8941 cttacaacgc tattacgcgt cagttagcgg atattattca ttgcaatgcc tgcattatca
     9001 atagtaaggg acgtctgctt ggctatttta tgcgttataa aacaaataca gatcgcgtag
     9061 agcaattctt ccaaactaag attttcccag atgactacgt tcaaggggct aatatgattt
     9121 acgaaacaga agcaaacttg cctgttgagc atgatatgag tattttccct gttgagagta
     9181 gagatgattt tccagatggc ttgacgacta ttgcaccgat tcatgtatcg gggattcgcc
     9241 ttggttcttt gattatttgg cgtaatgata aaaaattcga agatgaggac ttggttcttg
     9301 ttgagattgc cagtaccgtt gttgggattc agcttcttaa cttccaacgt gaagaagatg
     9361 agaaaaatat tcgtcgtcgt actgctgtca ccatggcggt caataccctt tcttactccg
     9421 aactccgtgc tgtttcagca attttagggg aattaaatgg aaatgaaggg aagttgactg
     9481 cgtcagtgat tgcagatcgt atcggaatca ctcgctctgt gattgtcaat gctcttcgta
     9541 aacttgagtc tgcggggatt attgaaagtc gctcacttgg aatgaaggga acctatctta
     9601 aggtcttgat ttcagatatt tttgaagaag tgaagaaaag agattactaa tgacaaaggc
     9661 tttaatttcg attgattata cagaagattt tgttgctgat agtgggaaac taacagcagg
     9721 tgctccagct caggcgattt cggatgccat cagcaaggta actcgattag cttttgaacg
     9781 aggagattat atcttcttta ctattgatgc tcatgaagaa aacgattgtt tccatccgga
     9841 aagtaagtta tttcctcctc ataatctgat tgggacgagt ggacggaatt tatatggaga
     9901 tttggggatc ttttatcaag agcatggttc agacagtcgt gtcttttgga tggataaacg
     9961 ccattactca gctttttcag ggactgacct agatattcgt ttgagagagc gtagagtgtc
    10021 taccgttatc ttaacaggtg tcttgacgga tatctgtgtc ctacatacag ctatagatgc
    10081 ctataatcta ggatatgaca tcgagattgt taaaccagct gttgcttcca tctggcctga
    10141 aaatcatcaa tttgccctag gtcatttcaa aaatacactc ggagctaagt tagtagatga
    10201 aaatctaaat gaactttctg agtaacctgg ttggtgaaaa tgaaaaaatt tgaaaaaaag
    10261 tgttgacaag catcccaaaa gttgatatac tagtaaagta atcgatgcgg ggatggcgga
    10321 attggcagac gcgcaggact aaggatcctg tgaccgcttt aggtcgtgag ggttcaagtc
    10381 cctctctccg cataggaaaa gagttagcta aagctagctc ttttgtattt ctctaaaaaa
    10441 ttagaatcca gtcaaatgta gtggagaatc agcgaaaata tgggaaaact gatccttgtt
    10501 tctgcttttg tctatagaag aataataaag attatcttct tcaaattctc cgatattctc
    10561 taaagttttg tgcaagttgc acagaacttg tttatttttt tggtcatctt gccatagaaa
    10621 tataaagcgt tttcatatat aatataatta ttaaaagaca aaaggagttc acctcatggt
    10681 agaattgaat cttaaaaata tttacaaaaa atatccaaac agcgaacact attcagttga
    10741 agatttcaac ttgaacatca aagataaaga atttatcgtt ttcgtaggac cttcaggatg
    10801 tggtaaatca actacactcc gtatgattgc tggtcttgaa gacattacag aaggtactgc
    10861 atctatcgat ggcgtagttg tcaacgacgt agctccaaaa gaccgtgata tcgccatggt
    10921 attccaaaac tacgctcttt acccacacat gactgtttat gacaacatgg ctttcggttt
    10981 gaaattgcgt aaatacagca aagaagacat taacaaacgt gttcaagaag cagctgaaat
    11041 acttggattg aaagaattct tggaacgtaa accagctgac ctttcaggtg gtcaacgtca
    11101 acgtgttgcc atggggcgtg cgattgtccg tgatgcgaaa gtattcttga tggacgaacc
    11161 tttgtcaaac ttggatgcca aacttcgtgt atcaatgcgt gctgaaatcg ctaaaattca
    11221 ccgtcgtatc ggagctacaa ctatctatgt aactcacgac caaacagaag cgatgacact
    11281 tgcagaccgt atcgttatta tgtcagctac taagaaccct gctggtacag gtactatcgg
    11341 acgtgtagaa caaatcggta ctcctcaaga agtttacaaa aatccagtta acaaattcgt
    11401 tgcaggattc atcggaagcc cagctatgaa cttcatcacc gtgaaattgg ttggtagcga
    11461 aattgtttct gacggtttcc gtttgaaagt gccagaagga gcattgaaag ttcttcgtga
    11521 aaaaggctac gaaggaaaag aattgatctt tggtatccgt ccagaagacg tgaatgcaga
    11581 acctgctttc cttgaaacat tcccagactg tgttgtaaaa gcgactatct ctgtatcaga
    11641 actgcttggt tcagaatctc acctttactg ccaagttggt aaagacgagt ttgttgcaaa
    11701 agttgatgct cgtgactact tgcaaacagg tgcaacagtt gagcttggat ttgacttgaa
    11761 caaagcacac ttcttcgatg tagaaactga aaaaacaatc tactaaaata aataaaattc
    11821 aaagcactac aagaaaagat atctctttat caattgtagt ggagagatat cagttaatct
    11881 agggagagaa acaaaatgct tctctccttt ttgctaggga agtcatatta tgcatctata
    11941 ttgtgatgct ctttaatact cttcgaaaat ctcttcaaac cacgtcaacg tcgccttgcc
    12001 gtacgtatga ttactgattt cgtcagtttt atctgcaacc tcaaagatgt actttgagca
    12061 gcttacggct agtttcctag tttgctcttt gatttccatt gagtattatt tgtgggtacc
    12121 atctacaagt gaagctatat gcgtaaacta cgtgagcaat tgaattcgaa ctagagaggt
    12181 aataataaat ttatgctata gttatggtga cttgtatgct tttgattcta gtttatcaaa
    12241 taatagatta gaattgtcag ataatatcat tttgtgttat aatgaagaaa aaacagaggt
    12301 gttcaaatgt cagaagcagg tcataagttt ttagcaaaat tggggaaaaa acgcttacgt
    12361 ccaggtggaa agcgtgccac agattggtta attgcagaag gaggattttc aaaagaaaag
    12421 agaatactag aggttgcgtg taatagggga actacagcaa ttgagttggc acagcgtttt
    12481 ggttgcaaga taactgctgt tgatatggat gctcaagctt tagaagtggc taaaaaatct
    12541 gctggaacgg caggtgttgc tcatttaatc agttttgaaa gagcaaatgc aatgaaactt
    12601 ccttatcaag atgctagttt tgatattgtt ataaatgaag ctatgctgac tatgcaagcc
    12661 gatcaagcta agaaaaaatg tgtaatggaa tatctaaggg tattaaaacc tggaggtctt
    12721 ctcttgacac atgatgtgct tcttaaggaa gctaaagagt ctatcagaca ggaattatca
    12781 caagcaattc atgtaaatgt aggtccttta actcaagatg gttgggaaca ggtgatgata
    12841 gaatcaggtt attgtgatgt gaaagcattg actggtgaaa tgacattaat gaaattatcg
    12901 ggtatgattt atgacgaagg tttgctagga actttgaaaa tttgtgtaaa tgcttgtaaa
    12961 aaggagaata gaaagcagtt tttaactatg tataaaatgt ttgctaagaa taaacagaaa
    13021 ttgggcttta ttgcgatggc tagttataaa tcgtcaaaac gttagataat tattgaagtt
    13081 aacttttcct tttttctttc ttaaaaaata tgctataata gagagtaaaa aactttgaaa
    13141 gaaagaaaaa gatgaattta aaagattaca ttgcaacaat tgaaaattat ccaaaggaag
    13201 gcattacctt ccgtgatatt agtcctttga tggctgatgg aaatgcttat agctacgctg
    13261 ttcgtgaaat cgttcagtat gctactgaca agaaagtcga catgatcgtg ggacctgaag
    13321 ctcgtggatt tatcgtgggt tgtccagttg cctttgagtt gggaattggt tttgcgcctg
    13381 ttcgtaagcc aggtaaattg ccacgcgaag ttatttctgc tgactatgaa aaagagtacg
    13441 gtgtcgatac cttgactatg cacgcggatg ccattaagcc aggtcaacgt gttcttattg
    13501 tagatgacct tttggcgaca ggtggaactg ttaaggcaac tatcgagatg attgaaaaac
    13561 ttggtggtgt tatggcaggt tgtgccttcc ttgttgaatt ggatgaattg aacggccgtg
    13621 aaaaaattgg tgactacgac tacaaagttc ttatgcatta ttaatgaaaa cagtccctag
    13681 ggctgttttc tctacactag gatataaaaa tagactataa ctagttagag aaaaactata
    13741 attgaaaact atatcttctt acagtataat aaaaggacta agtgtttgag atttgtcttc
    13801 aaacatatgc aattattcct gaaagagtac agttaggaga gggttatgcc gattcgaatt
    13861 gataaaaaat tgccagctgt tgagatttta cggacagaga atatctttgt catggatgat
    13921 caacgtgctg cccaccaaga tatccgtcct ttgaagattt taattttaaa tctcatgcca
    13981 cagaaaatgg tcacagagac ccagttgttg cgccacttgg ctaatacacc cctacaactg
    14041 gatattgatt ttctctatat ggagagccac cgttctaaaa caactcgttc agagcacatg
    14101 gagaccttct ataaaacttt tcctgaagtc aaggatgagt attttgatgg gatgatcatc
    14161 acgggtgctc cagttgagca tttaccattt gaggaagtgg actattggga ggaatttaga
    14221 cagatgcttg agtggtctaa gactcatgtc tattcgaccc ttcatatctg ttggggggct
    14281 caggctgggc tttatctgcg ctatggtgta gaaaaatacc agatggacag taagctatca
    14341 ggtatttatc ctcaggacac cctaaaagag ggtcaccttc tatttagagg ctttgatgat
    14401 agctatgtat cccctcattc acggcacacg gagatttcta aggaagaggt cttaaacaag
    14461 accaatctcg agattttatc agaaggacct caggttgggg tttctatttt ggccagtcgt
    14521 gatttacgag aaatttatag ttttggtcat ttggagtatg accgtgatac cttggcaaaa
    14581 gagtattttc gagatcgtga tgcaggtttc gacccacata ttccagaaaa ttactttaaa
    14641 gatgatgatg ttaatcaggt accttgtctt tgttggtctt catctgcagc cctctttttc
    14701 agtaattggg taaaccatgc ggtctatcag gagacgcctt ttgattggat aaagatagaa
    14761 gatgatgcat ctgcatatgg gtatttataa gaggaattat gacatattta gacgctttta
    14821 aatcaggtac cttggtttta ccgagtgccc tgctcttgca ttttaaggaa ctctttcctt
    14881 ctagcgacga ttttctggtt tggcaatttt tctatttgca aaatacgaca ggcttagaag
    14941 aaatgtcgcc aagccagatt gctgaaagga ttggcaagga aatttcggat gtcaaccagt
    15001 ccatttctaa tctgacggaa aggggactgc tccagtatcg tactatcgaa ttaaatggcg
    15061 aaattgaatt gctctttgat gctagtttgg ccttggaacg tttggatgac ctgtttggag
    15121 cagttcattc aagttcagac cagctaacac ctcaaaacca gctcaaggat ttggtggaaa
    15181 ccttccagca ggagttggga cgattgttga cgccttttga gattgaggat ttgaccaaga
    15241 cactaaagga agatggaacc agtgctgact tgattaagga ggctcttcgt gaagctgttt
    15301 tgaatggaaa accaaactgg aagtacattc aggcgatttt gagaaactgg cgccatgaag
    15361 gaatcaagag tgtggctcaa attgaggcca agagagcaga aagagaagca agcaatcctc
    15421 agttgacaca ggtatctgca gatttcagaa atgccatgga tctctggaag gattaatcca
    15481 tgcaagcagg cttgaaatcc gagtaagatt tgcaagctgt gtataattgt gatagaataa
    15541 atagaaaata aattgaaaaa gaggtatgtg aaatgtcacg taaaccattt atcgctggta
    15601 actggaaaat gaacaaaaat ccagaagaag ctaaagcatt cgttgaagca gttgcatcaa
    15661 aacttccttc atcagatctt gttgaagcag gtatcgctgc tccagctctt gatttgacaa
    15721 ctgttcttgc tgttgcaaaa ggctcaaact ttaaagttgc tgctcaaaac tgctactttg
    15781 aaaatgcagg tgctttcact ggtgaaacta gcccacaagt tttgaaagaa atcggtactg
    15841 actacgttgt tatcggtcac tcagaacgcc gtgactactt ccatgaaact gacgaagata
    15901 tcaacaaaaa agcaaaagca atctttgcga acggtatgct tccaatcatc tgttgtggtg
    15961 aatcacttga aacttacgaa gctggtaaag ctgctgaatt cgtaggtgct caagtatctg
    16021 ctgcattggc tggattgact gctgaacaag ttgctgcctc agttatcgct tatgagccaa
    16081 tctgggctat cggtactggt aaatcagctt cacaagacga tgcacaaaaa atgtgtaaag
    16141 ttgttcgtga cgttgtagct gctgactttg gtcaagaagt cgcagacaaa gttcgtgttc
    16201 aatacggtgg ttctgttaaa cctgaaaatg ttgcttcata catggcttgc ccagacgttg
    16261 acggtgccct tgtaggtggt gcgtcacttg aagctgaaag cttcttggct ttgcttgact
    16321 ttgtaaaata atcagtaagt agcaaaagct aggtggaaca gcattcagat gtctgttaca
    16381 ttttttatag gagaggaaag attgaaaaca aaaattggat tagcaagtat ctgtttacta
    16441 ggcttggcaa ctagtcatgt cgctgcaaat gaaactgaag tagcaaaaac ttcgcaggat
    16501 acaacgacag cttcaagtag ttcaaagcaa aatcagtctt ctaataaaac gcaaacgagc
    16561 gcagaagtac agactaatgc tgctgcccac tgggatgggg attattatgt aaaggatgat
    16621 ggttctaaag ctcaaagtga atggattttt gacaactact ataaggtttg gttttatatt
    16681 aattcagatg gtcgttactc gcagaatgaa tggcatggaa attactacct gaaatcaggt
    16741 ggatatatgg cccaaaacga gtgaatctat gacagtaatt acaagagttg gttttatctc
    16801 aagtcagatg gggcttatgc tcatcaagaa tggcaattga ttggaaataa gtggtactac
    16861 ttcaagaagt ggggctatat ggctcggaat gagtggcaag gcaactacta tttgactgga
    16921 agtggtgcca tagcaactga cgaagtgatt atggatggtg ctcgctatat ctttgcggcc
    16981 tctggtgagc tcaaagaaaa gaaagatttg aatgtcggct gggttcacag agatggtaaa
    17041 cgctatttct ttaataatag agaagaacaa gtgggaaccg aacatgctaa gaaagtcatt
    17101 gatattagtg agcacaatgg tcgtatcaat gattggaaaa aggttattga tgagaacgag
    17161 gtggatggtg tcattgttcg tctaggttat agcggtaaag aagacaagga attggcgcat
    17221 aacattaagg agttaaaccg tctgggaatt ccttatggtg tctatctcta tacctatgct
    17281 gaaaatgaga ccgatgctga gaatgacgct aaacagacca ttgaacttat aaagaaatac
    17341 aatatgaacc tgtcttaccc tatctattat gatgttgaga actgggaata tgtaaataag
    17401 agcaagagag ctccaagtga tacaggcact tgggttaaaa tcatcaacaa gtacatggac
    17461 acgatgaagc aggcgggtta tcaaaatgtg tatgtctata gctatcgtag tttattacag
    17521 acgcgtttaa aacacccaga tattttaaaa catgtaaact gggtagcggc ctatacgaat
    17581 gctttagaat gggaaaaccc tcattattca ggaaaaaaag gttggcaata tacctcttct
    17641 gaatacatga aaggaatcca agggcgcgta gatgtcagcg tttggtatta agcgatgatt
    17701 tgaaagaggg atgtgatagt agcaccctct ttttctttgt tttatgatag ttcatcctcg
    17761 agtaaattca agatcttgct cggaaatgaa gcttatatag tagattgaat atagacaaag
    17821 accttgtgat tggtaaaaca ttttagaaat tcatttacct ttcctaatcg acttggtttc
    17881 atcttatttc aatctattat agtattgggg aatttcttca aaccacatca gcttggtcag
    17941 ttctacctgc gacctcaaaa cttgtgcttt ggtcaagctg ggtttagttt cctagtttgc
    18001 tgatggattt ccattgacta taagcatcca accctctttt ttgtcttcta aagaattctt
    18061 aaattatcag tctattgcaa cttttctcat ataagttctt tgtcttgcta ttggttttcc
    18121 ttagtagtat actaaggtag taatcattaa gaagtggtta caaaaaataa tgaatgaggt
    18181 aaagaaaatg gtagaattga aaaaagaagc agtaaaagac gtaacatcat tgacaaaagc
    18241 agcgccagta gcattggcaa aaacaaagga agtcttgaac caagctgttg ctgatttgta
    18301 tgtagctcac gttgctttgc accaagtgca ctggtatatg catggtcgcg gtttccttgt
    18361 atggcatcca aaaatggatg agtacatgga agctcttgac ggtcaattgg atgaaatcag
    18421 cgaacgcttg attacactcg gtggaagccc attctctaca ttgacagagt tccttcaaaa
    18481 tagtgaaatc gaagaagaag ctggtgaata ccgtaatgtt gaagaaagct tggaacgtgt
    18541 tcttgttatc taccgttact tgtcagaact tttccaaaaa ggtttggatg tcactgatga
    18601 agaaggtgac gatgtgacaa acggtatctt tgcaggcgct aaaactgaaa cagataaaac
    18661 aatttggatg cttgcagccg aacttggaca agcacctggt ttgtaagaaa taaggaaaca
    18721 tagataaatc tgtaggaaat ttcttacaga ttttttctat tttgtaagct attccaaacc
    18781 agtctttttt tgagtttttt gataaaatag tactatcagt gaaaaggatg gaagcatgac
    18841 taagaaaatc gtagctattt gggcccagga tgaagagggt gtgattggta aggaaaatcg
    18901 tctgccttgg catttgccag cagaattgca gcactttaaa gaaacaactc tgaatcatgc
    18961 tatcttgatg gggcgtgtga cctttgatgg gatggggcgt cgcttgcttc caaaacggga
    19021 aaccttgatt ttgacgcgta atccggaaga aaagatagat ggggttgcta cttttcagga
    19081 cgtccagtct gttcttgact ggtatcaggt tcaagaaaag aatctctaca ttatcggtgg
    19141 gaagcaaatt tttcaggctt ttgaacctta ccttgatgaa gtgattgtca ctcacattca
    19201 tgctcgggtg gaaggagata cctatttccc taaagaactt gacttgtctc tttttgagac
    19261 agtttcaagc aaattttacg ccaaagatga gaagaatcct tatgatttta ccatccaata
    19321 ccgcaagaga aaggaagtct aatggaacgg agtttatttg gtctattcac ggcttttctg
    19381 tgtttcatct gctttctggc aggagcacaa gcttttcgta aaaagcgtta cgggctttct
    19441 atcctccttt ggttaaatgc ctttaccaat ctggtcaata gtattcatgc tttttacatg
    19501 accttatttt agatagaatg acagtataga atggaaggaa atcatgtcta caaatagaaa
    19561 aaatgatatg atggtttatt gctcattttg tggcaaaaac caagaagaag tacaaaaaat
    19621 aattgctggc aacaatgctt ttatttgtaa tgaatgcgtg gagttggctc aggaaatcat
    19681 tcgagaagaa ttggttgagg aagtcttggc agacttgtct gaggtgccaa aaccaattga
    19741 actcctccat atcttgaacc actatgtaat tggtcaagat cgtgccaagc gtgccttggc
    19801 agtggcggtt tataaccact acaaacgcat caatttccac gatacacgcg aagagtcaga
    19861 agatgtggat ttgcagaagt caaacatttt gatgattggc ccaactggtt cagggaaaac
    19921 tttccttgcc cagaccttgg ctaagagctt gaatgtacct tttgctattg cggatgcgac
    19981 agctctgacg gaggctggtt atgtgggtga ggacgtggaa aatatcctcc tcaaactctt
    20041 gcaggttgct gactttaaca tcgaacgtgc agagcgtggc attatctatg tggatgaaat
    20101 tgacaagatt gccaagaaga gtgagaatgt gtctatcaca cgtgatgttt ctggtgaagg
    20161 ggtgcaacaa gcccttctca agattatcga gggaactgtt gctagcgtgc cgcctcaagg
    20221 tggacgcaaa catccacaac aagagatgat tcaagtggat acaaaaaata tcctcttcat
    20281 cgtgggtggt gcttttgatg gtattgaaga aattgtcaaa caacgtctgg gtgaaaaagt
    20341 catcggattt ggtcaaaaca ataaggcgat tgacgaaaac agctcataca tgcaagaaat
    20401 catcgctgaa gacattcaaa aatttggtat tatccctgag ttgattggac gcttgcctgt
    20461 ttttgcggct cttgagcaat tgaccgttga tgacttggtt cgcatcttga aagagccaag
    20521 aaatgccttg gtgaaacaat accaaacctt gctttcttat gatgatgttg agttggaatt
    20581 tgacgacgaa gcccttcaag agattgctaa taaagcaatt gaacggaaga caggggcgcg
    20641 tggacttcgc tccatcatcg aagaaaccat gctagatgtt atgtttgagg tgccgagtca
    20701 ggaaaatgtg aaattggttc gcatcactaa agaaactgtc gatggaacgg ataaaccgat
    20761 cctagaaaca gcctagaggt gactatggaa cttaatacac acaatgctga aatcttgctc
    20821 agtgcagcta ataagtccca ctatccgcag gatgaactgc cagagattgc cctagcaggg
    20881 cgttcaaatg ttggtaaatc cagctttatc aacactatgt tgaaccgtaa gaatctcgcc
    20941 cgtacatcag gaaaacctgg taaaacccag ctcctgaact tttttaacat tgatgacaag
    21001 atgcgctttg tggatgtgcc tggttatggc tatgcccgtg tttctaaaaa ggaacgtgaa
    21061 aagtgggggt gcatgattga ggagtactta acgactcggg aaaatctccg tgcggttgtc
    21121 agtctagttg accttcgtca tgacccgtca gcagatgatg tgcagatgta cgaatttctc
    21181 aagtattatg agattccagt catcattgtg gcgaccaagg cggacaagat tcctcgtggt
    21241 aaatggaaca agcatgaatc agcaatcaaa aagaaattaa actttgaccc aagtgacgat
    21301 ttcatcctct tttcatctgt cagcaaggca gggatggatg aggcttggga tgcaatctta
    21361 gaaaaattgt gaggaaaaga aaatggcaaa aacaattcat acagataagg ccccaaaggc
    21421 tatcggaccc tatgttcaag gaaaaatcgt tggcaacctt ttgtttgcta gcggtcaagt
    21481 tcccctatcc cctgaaactg gagaaattgt aggagagaat atccaagaac agacagagca
    21541 agtcttgaaa aacatcggtg ctattttggc agaagcagga acagactttg accatgttgt
    21601 caaaacaact tgcttcttga gcgatatgaa cgactttgtt ccttttaatg aggtttacca
    21661 aacggccttc aaagaggaat tcccagctcg ttcagctgtg gaggtagctc gtcttcctcg
    21721 tgatgtaaaa gtcgaaattg aagtcatcgc agagattgga taagctagtt gaagtttggt
    21781 gttgccaaac ttcttttgat ataaggagaa aaagatgaca aagaaacaac ttcacttggt
    21841 gattgtgaca gggatgagtg gcgcagggaa aactgtagcc attcagtcct tcgaggatct
    21901 aggttatttc accattgata atatgccgcc agctctcttg cctaagtttt tgcagctggt
    21961 tgaaattaag gaagacaatc ctaagttggc cttggtagtg gatatgcgta gccgttcttt
    22021 cttttcagag attcaagctg ttttggatga gttggaaaat caagatggtt tggatttcaa
    22081 aatcctcttt ttggatgcgg ctgataagga attggtcgct cgttacaagg aaaccagacg
    22141 gagtcaccca ctagcagcag acggtcgtat tttagatgga atcaagttgg aacgtgaact
    22201 cttggcacct ttgaaaaata tgagccaaaa tgtggtggat acgactgaac tcactccacg
    22261 tgagctgcgc aaaacccttg cagagcagtt ttcagaccaa gaacaagccc agtctttccg
    22321 tatcgaagtc atgtctttcg gatttaagta tggaatcccg attgatgcgg acttggtctt
    22381 tgatgtccgt ttcttgccaa atccctatta tctaccagaa ctgagaaacc aaacgggtgt
    22441 ggatgaacct gtttatgatt atgtcatgaa ccatcctgag tcagaagact tttatcaaca
    22501 tttattggcc ttgattgagc cgattctgcc aagttaccaa aaggaaggta agtccgtttt
    22561 gaccattgcc atgggatgta cgggtggaca acaccgtagt gtggcatttg ctaaacgctt
    22621 ggcgcaggac ttatccaaga attggtctgt taatgaaggg catcgcgaca aagaccgcag
    22681 aaaggaaacg gtaaaccgtt catgagaaaa ccaaagataa cggtgattgg tggagggact
    22741 ggaattcccg tcattctaaa aagtctgcgg gaaaaagatg tggaaatcgc agctatcgtg
    22801 acggtggcag atgatggtgg ttcttcaggt gaactccgaa aaaatatgca acagttgaca
    22861 ccgccaggtg atcttcgtaa tgtccttgtg gccatgtcgg atatgcctaa gttttatgag
    22921 aaggtctttc agtatcggtt ctctgaggat gccggagcct ttgctggcca tccattggga
    22981 aatctcatca ttgctggctt gtcagaaatg cagggttcaa cctataatgc catgcagtta
    23041 ttgagcaaat ttttccatac aacagggaaa atttatcctt ccagtgacca tcctttgacc
    23101 cttcatgcag tctttcagga tgggacagaa gtggctggag agagtcatat tgtagaccat
    23161 agaggcataa ttgacaatgt ctatgtgacc aatgccctaa actatgatac gcctctggcc
    23221 agccgtcgag tagtgcagac catccttgaa agtgacatga ttgtcctagg gccaggttcc
    23281 ctctttacct ctattttgcc caatatcgtg attaaggaaa ttgggcgggc tcttttagaa
    23341 accaaggcag aaattgccta tgtctgcaat atcatgaccc aacgtgggga gacggaacac
    23401 tttacagata gcgaccacgt ggaagtcttg catcgtcacc ttggtcgccc ttttatcgac
    23461 actgtcttgg tgaatattga aaaagtgcct caggaataca tgaattccaa ccgttttgat
    23521 gaatacttgg tgcaagtgga acacgatttt gtaggtcttt gtaagcaagt ttcgcgcgtg
    23581 atttcatcta acttccttcg tctggaaaat ggcggtgcct tccacgatgg agatttgatt
    23641 gtggacgagt tgatgcgcat tatacaggtg aaaaaatgag tttcacagta gcagtaaaag
    23701 aagaaatcct gggccaacac catctgagcc ggcatgaatt atctgccatt atcaaaatgt
    23761 ctggtagcat cggtctctcg acttcgggct tgactttgtc agttgtgaca gaaaatgcca
    23821 aactggcccg tcacctctat gagtcctttc tccatttcta tgaaatcaaa tctgaaattc
    23881 gtcaccacca acggagcaat cttcgcaaga atcgcgtcta taccgttttt acagatgaaa
    23941 aggtgcagga tttgttaagt gatttgcact tggcagactc tttctttggc ctggaaacag
    24001 gtattgatga ggcgatttta tcggatgagg aagcaggtcg tgcctatctc tgtggcgctt
    24061 tcttggcaaa tgggagcatt cgtgaccctg agtcaggcaa gtaccagttg gaaatcagtt
    24121 ctgtttatct ggaccacgcg caaggaattg cctcccttct ccaacagttt ttactggatg
    24181 ccaaggtgct tgagcgcaag aagggggctg tgacctatct ccagcgagca gaagacatta
    24241 tggacttctt gatagtaatc ggagccatgc aggcacgtga tgattttgag cgggttaaga
    24301 ttttgcgaga aacccgtaac gacctcaatc gggctaataa tgccgagaca gctaatatcg
    24361 ctcggacagt ttctgccagc atgaagacca tcaacaatat cagtaaaatc aaagatatca
    24421 tgggcttaga aaatttgcca gtagatttgc aggaagtggc gcaactgcgg attcagcacc
    24481 cagactactc tatccaacag ttggcagata gcctcagcac ccctctgacc aaaagtggtg
    24541 tcaatcacag actcagaaaa atcaataaga tagcggatga attataaaac cacgaatcct
    24601 atgtgactcg tggttctttt ttataaactg gtagagtgtt ttggttgtac tttttgttca
    24661 gggtcaatgt agttgatagc gttgttgaca gcagttggag cttctccgag gcctgtcgca
    24721 atcagatcaa tttttccgtc atagtagcag cagtcaccga tagcatagat acctgcttgg
    24781 ctggattcct gtttgctgtt gacgataatc ttgtgacggt tgaggtcgag cccccagttt
    24841 ttaaggttac cgacagaaga tttgaaacca tagttgacaa agaggtggtc tagatcaatg
    24901 gtttcagttt catcagattt gacttttgtg atttcaagtt tatcaagtgt ttttccattt
    24961 ccaaggagtt ggctaggggc gaatggtgtc ttgatggtta cagatgattc ttgcaaggct
    25021 tgaacactgt gttccaaggc acggaaatta tctctgcggt gaacaagggt agttggtgcg
    25081 attttttcaa aagccaaagc ccaatccaca gccgagtctc ccccaccaag aatcgtcact
    25141 ttcttaccag cgtattgctg aatgttagaa acgtggtagt ggatattttc atagccctca
    25201 accccttcaa gttccagcgg acgtggtttg aaggcaccgc cacccatagc gatgataact
    25261 gttttagtca ggtgacttcc tttagaagtt gtgatggcaa attcttcttg tttgtcaatc
    25321 tcaagaaccg tttcattgag atgaataggg gtatcaaatc catttagctg ttcaatcaag
    25381 cggttagtca actcttctcc agtcaggttt gggaagcctg gtacgtctag gatttccttt
    25441 tcagggtaga gaatagcagg ttgtccacct agctggggaa gagagtcgat gatttgaacc
    25501 ttggcttggc gtaggtgggc ataaaaggct gcaaaaagcc cgataggacc accacccaca
    25561 atggtaatat catagagttg agacatagtt tctcctttgt tttttctagt cagtttattt
    25621 tatcatattt ttgttcaatg taaaatggac taggataggg aaaaaaatgc cagaaaatgg
    25681 tataatagga cgaaagtgtt ttgataggga ggatatcagg gatgaatttt caacaattat
    25741 ccaatctgca atattggacc agtttgtttg caagtccatg gacgatagct atcaatctga
    25801 ttgatatttt gattgttgct tatattttat accattttac aaaagctatt gcaggaacca
    25861 agattatgat tttggtacgt ggagttttgg tgtttatttt agctcaaatc cttgcaaata
    25921 tgattggttt gactacgatt tcttggttaa tcaatcaaat tattacttat ggggttattg
    25981 cggcggttgt tatcttctct ccagagattc ggactggttt ggaacgtttg ggaagagcga
    26041 cagatttctt ttccaatgcc cctattagtg ctgaggaaca gatgattcgt gcctttgtta
    26101 agtctgttga atacatgagt cctcgtaaaa tcggggcctt ggttgctatt cagcgtgtac
    26161 gtaccttgca ggagtatatt tcgacaggaa ttcccttgga tgctaatatt tctgcagaac
    26221 ttctcattaa catttttatt cccaacactc ccctacatga tggtgcggtg attatcaaag
    26281 aagaacgtat cgctgtgacg tctgcctatc tgcccttgac aaaaaacaca gggatttcca
    26341 aggaatttgg gaccagacac cgggcggcta tcggtttatc agaagtctca gatgccttga
    26401 cttttgtcgt atcagaggaa acgggaggaa tttcgataac ctataatgga aggtttaagc
    26461 acaacctaac acttgatgaa tttgaaacag aattacgtga aatcttactt ccaaaagagg
    26521 aagtgggtct tagttttaaa gaacgattgc taggaggatg gaaacatgaa aaaaaatagt
    26581 ttatatatca tatcctcact cttttttgct tgtgtcttat ttgtctatgc tacggcgacg
    26641 aattttcaaa acagtaccag tgctaggcag gtaaaaacgg aaacctatac taatacagta
    26701 acaaatgtcc ctattgacat acgctataat agtgataagt attttattag cggttttgct
    26761 tcagaagtat cagtggtctt gactggtgca aatcgcctat cgctagctag tgaaatgcaa
    26821 gaaagtacac gtaaattcaa ggttactgct gacctaacag atgccggtgt tggaacgatt
    26881 gaagttcctt tgagcattga agatttaccc aatgggctga ccgctgtggc gactccgcaa
    26941 aaaattacag tcaagattgg taagaaggct cagaaggata aggtaaagat tgtaccagag
    27001 attgacccta gtcaaattga tagtcgggta caaattgaaa atgtcatggt gtcagataaa
    27061 gaagtgtcga ttacgagtga ccaagagaca ttggatagaa ttgataagat tatcgctgtt
    27121 ttgccaacta gcgaacgtat aacaggtaat tacagtggtt cagtaccttt gcaggcaatc
    27181 gaccgcaatg gtgttgtctt accggcagtt atcactccgt ttgatacaat aatgaaggtg
    27241 actacaaaac cagtagcacc aagttcaagc acatcaaatt caagtacaag cagttcatcg
    27301 gagacatctt cgtcaacgaa agcaactagt tcaaaaacga attaaaaata gaaaaggatt
    27361 ttataacaat gggtaaatat tttgggactg atggagtccg tggagaagct aacctagaac
    27421 taacaccaga attagccttt aaactaggac gttttggagg ctatgttctt agtcaacatg
    27481 aaacggaagt gccgaaagtc tttgtaggac gtgacacacg tatttcaggg gaaatgctgg
    27541 aatcggcctt ggtggcaggt ctcctttcag tagggattca cgtatacaaa cttggtgtcc
    27601 ttgcaacatc agcagtagct tacttggttg aaactgaagg agcaagtgcc ggtgtcatga
    27661 tttctgctag ccacaaccca gcccttgata acggaatcaa gttctttggc ggtgatggct
    27721 tcaaactaga tgatgaaaaa gaagcagaaa ttgaagcctt gctagatgct gaggaagaca
    27781 ctcttcctcg tccaagtgca gaaggtttag gaatcttggt agattatcca gaaggcttgc
    27841 gtaagtatga aggatacctt gtttcaactg gaactcctct tgatggaatg aaggttgcct
    27901 tggatacagc taatggagca gcttctacca gtgcccgtca aatctttgca gaccttggtg
    27961 cccaattgac ggttatcggg gaaacaccag acggtcttaa catcaacctt aatgttggtt
    28021 caacacatcc agaagccctt caagaagtgg tcaaagaaag tgggtcagct attggtttgg
    28081 cctttgatgg agacagtgac cgcttgattg ctgttgatga gaatggtgac atcgtcgatg
    28141 gtgacaagat tatgtacatc atcggaaaat acctttctga aaaaggacaa ttggctcaaa
    28201 atacaattgt gacaactgtt atgtctaacc ttggtttcca caaggccttg aatcgcgaag
    28261 gtattaacaa ggcagttact gcagttggtg accgctacgt tgttgaagaa atgagaaaat
    28321 caggctacaa ccttggtggt gaacagtctg gtcacgttat cttgatggat tacaatacca
    28381 caggtgatgg tcaattatca gcagttcaat tgactaaaat tatgaaggaa actggtaaga
    28441 gcttatcaga gttggcggca gaagtaacga tttatccaca aaaattagtt aatatccgag
    28501 tggaaaacgt catgaaggaa aaggccatgg aagtgccagc tatcaaggcc atcatcgaga
    28561 agatggaaga agaaatggcg gggaacggcc gtatccttgt tcgtccaagt ggaacagagc
    28621 ccctcttgcg tgttatggca gaagcgccta caacagaaga agtggactac tatgttgata
    28681 ccatcacaga tgtagttcgt gctgaaattg ggattgacta aatcctagaa aactagaaga
    28741 aaataaaaaa ttatggtaca atagcttata atattgtagc cgatggagaa agacatgaat
    28801 cttaaacgtg aacaagaatt tgttagtcag tatcattttg atgcgcgtaa ttttgaatgg
    28861 gaaaatgaaa atggagcccc tgaaaccaag gtggatgtga atttccaatt gctccaacat
    28921 gaccaagaaa atcaagtgac ttccttgatt gtcatcttga gctttatgat tgtatttgat
    28981 aagtttgtca ttagtggaac aatctcacaa gtaaaccata tcgatggccg tattgtcaat
    29041 gaaccaagtg aattgaacca agaagaggtg gaaaccttgg ctcgtccatg tttgaacatg
    29101 ctcaatcgtt tgacttacga agtgactgaa attgcattag acttaccagg aattaatttg
    29161 gagttctaat atgaaattag ctgttttcac agattcctct gcttatctca gtgaagagac
    29221 cttgcaaaga gaagacttgt ttgtcttgga tattcctgtc aatattgatg gtgaggaata
    29281 tgtcgaaggc attaatctgt ctgctgagga attttaccaa aaaatggctc aggcttctga
    29341 attgcctaag accagtcaac caagtattgc caagttagat gaaatcttaa cttcgctcaa
    29401 agaacaaggc tatacacatg ccttggggct tttcctatct tctggaattt caggttttta
    29461 ccaaaatatc cagtatatgg tcgatgacta tgagggctta accattgctt tcccagacac
    29521 tttgattaca agtgctcccc taggcatcat ggttgaaagc gtctttaatt ggcgtgatca
    29581 gggcgatgat tttgccagca ttcaggataa gctagccatt caaatcagcc gtacgtcagc
    29641 ctttatcatg gtagatgact tggatcattt ggtaaaaggt ggacgccttt caaatggggc
    29701 tgccattttg ggcaatttgc ttagcattaa gccaatcctt tattttaacg atcaaggtgt
    29761 gattgaagtt tacgaaaaag ttcgtactga aaagaaggcc accaagcgct taattgaaat
    29821 tatcaaggaa acaacggctt caggtcaata ccgggtcatt gtcattcatg ggaatgctcc
    29881 tgaaaaggct gaagaattgc gtcagcattt gcttgatttt ggcttgggtt cggatgtttc
    29941 acttgctaca tttggtagtg tcattggaac gcacctagga gcaggaagta ttgctctggg
    30001 ttatattcca gtgatttagt tggcacttct aggctagtta atcttagcaa ttgaacacgg
    30061 actacctgcc tcttgaaaaa agatgcctgt cttgcctttc gtggaaagtc agtgccattc
    30121 cctatttttc aggggcagct aaggtccttt gtatcttgat aattgaacac gcctggaacc
    30181 ctgtgtgaaa aagatagttc ttccaagaag tagacacttc ttgattggaa ttcctatttt
    30241 cactttgtgt tcttacggac tttgtatctt agacaggagt agagatgagt attcgagtaa
    30301 ttattgctgg ttttaaggga aagatgggcc aggctgcttg tcagatggta ttgactgatc
    30361 cagacttgga cttggtagca gttttggatc cttttgagtc tgagtcagaa tggcagggta
    30421 ttcctgtttt caaggataag gctgatttag ctggttttga agcggatgtc tgggtagatt
    30481 ttactactcc agctgttgcc tacgaaaata cacgttttgc tcttgaaaat ggctttgctc
    30541 cagtagttgg aacgactggt ttcacgagtg aagaaattgc agagctaaaa gaattttctc
    30601 gtgcccaaga cttgggtggc ctgattgccc ctaactttgc cttgggtgct gtcttattca
    30661 tgcaatttgc gacgcaggct gccaaatatt tcccaaatgt ggagattatt gagctccatc
    30721 atgacaagaa aaaggatgct ccgagtggaa cagccattaa aacagctgag ttgatggcag
    30781 aggttcgaga gtcaattcag caaggtgcag cagatgagga agagctgatt gctggtgctc
    30841 gtggtgctga ctttgatggt atgcgcatcc actcagttcg tttgccgggc ttggtagctc
    30901 atcaggaagt catctttggc aatcagggag aagggttgac cctccgtcat gactcctatg
    30961 atcgcatctc cttcatgaca ggagtcaatt tgggaattaa agaagttgtc aagcgtcatg
    31021 agcttgtcta tggattagaa cacttattat gagattaacg caaatgcctt ctgaatttca
    31081 gaaggcttta ccagtattag aaaaaattaa agaagcaggc tttgaggctt attttgttgg
    31141 gggctctgtt cgagatgccc ttctccatag ccctatccac gatgtggata ttgcgacgtc
    31201 ttcttatcca gaagagacca agcagatttt tccgcgaaca gccgatatcg gaatcgagca
    31261 tggaaccgtc ttggtcttag atggggatga ggagtatgag gtaacaacct ttcggacaga
    31321 ggatgtctat gtggactatc gcagacccag tgcggtttcc tttgtacgat cgctagaaga
    31381 agacctcaaa cgccgtgatt tcacagtcaa cgcctttgcc ttggatgaga caggagaaat
    31441 cgttgacttg ttccatggtt tagaagattt ggaaaagcaa gtcttgcgag cagttggagt
    31501 ggctagtgag cgtttcaacg aagatgcttt acggattatg cgtggtttcc gttttcaggc
    31561 tagtcttggt tttgcacttg agccagaaac atttaaagct atgaagacct tgacgccgct
    31621 tttggagaaa atttctgtag agcgtacctt cgtcgagttt gataaactct tgctggctcc
    31681 attttggaga aggggcttgg cttccatgat tgagagtcaa gcttatgact atctccctga
    31741 tatggcatct agccaggaca agctcaacag actgtttgat ttggagactg attttacttt
    31801 tgaatcctct gaacaagcct gggcggcttt actatgggct ttggagattg aaaatgcgca
    31861 gtcatttttg aaatcttgga agacctcacg ccagtttgcc aagcaagttc aggatttgct
    31921 gattattttg gctctgcgtg aaaatggaga attgagcaag cgagattgtt atcgctttga
    31981 catagatttg cttttacagg ctgaaaatct tcgtcaagct caaggaaaag aagtcaaccc
    32041 acaagccatc acagaaaaat accaaagctt gactatccat gacaagaaag agattcagat
    32101 taatggcgat attttgatca aggaatatgg ctatcagcca ggcccagact tgggagagat
    32161 tttaacagag attgagtttg ccattgtcga tggagaattg gagaataatc gtgaagccat
    32221 ccatgcttac ctgagggaga aaaaatgagt gattttatcg ttgaaaaact aagtaaatcc
    32281 gttggtgaca agaccgtttt tagggatatt tcctttatta tccatgactt agacagaatt
    32341 ggtttaatcg gtgtcaatgg gactggcaag accacccttt tggacgtcct ttctggtgtt
    32401 tctggatttg atggggatgt cagtcctttt tcagctaaaa atgattacca gattggttat
    32461 ttgactcagg atcctgattt tgatgataga aagacagttt tggatacggt tctatctagt
    32521 gaactcaagg aaatccagct cattcgtgag tatgaattga ttatgctcga ctatagtgag
    32581 gacaagcagg cgcgtttgga acgtgtcatg gcagagatgg actctctcca agcttgggaa
    32641 atcgagagtc aggtcaagac cgttcttaac aaattgggca ttcaagactt atctactcct
    32701 gttggggaat tgtcaggtgg tctgagaaga cgggtacagt tggcacaagt cttacttggc
    32761 aaccacgacc ttttgctttt ggatgagccg accaaccatc tggatattgc gattattgag
    32821 tggctgaccc tctttttgaa aaattctaag aagaccgtcc tttttatcac tcacgatcgt
    32881 tatttcttag acgctttgtc aacacggatt ttcgagttgg agcgagcagg cttgacagaa
    32941 tatcagggca attaccagga ctatgttcgc cttaaggcag aacaggacga gcgcgatgca
    33001 gctctacttc ataaaaaaga acaactctac aaacaagaac tggcctggat gagcagacaa
    33061 ccgcaggcgc gtgcgaccaa gcaacaagct cgtatcaatc gtttccatga tctgaaaaag
    33121 gaagtttcag gcagtagtgc tgagacagac ttgaccatga actttgaaac cagtcggatt
    33181 gggaagaaag tcatcgagtt tcaggatgtt tcctttgcct atgaaaataa gcccattttg
    33241 caagatttta atctcttagt tcaggctaaa gaccgtattg gaattgttgg ggacaatggt
    33301 gttggaaaat caaccctact taacctgatt gcaggaagtc ttgagccgac agcaggacaa
    33361 gttgtgattg gggaaactgt tcgcatcgcc tatttctctc aacaaattga gggtttggat
    33421 gaaagcaagc gtgtgatcaa ttacctgcag gaagtggcag aggaggtcaa gaccagtggt
    33481 ggttctacga cttccatcgc tgagttgctg gagcaattcc tcttcccacg ttcgacgcat
    33541 gggaccttga ttgagaaatt gtcagggggt gagaaaaaac gtctttatct cctcaaactg
    33601 cttttggaaa aaccaaatgt tcttctttta gacgagccaa ccaatgacct agatattgca
    33661 actttgacag tcttagagaa tttcttgcaa ggttttgcag gtcccgtttt aacagtcagt
    33721 cacgaccgct atttcttgga taaggtagcg accaagattc tcgcttttga ggatggcaag
    33781 attcgtcctt tctttggtca ttacaccgac tatcttgatg aaaaagcttt tgaaacagat
    33841 atggccaatc aagtgcaaaa ggccgaaaag gaaaaagtgg tcaaggttcg agaagacaag
    33901 aaacgcatga cctaccaaga aaagcaggag tgggcaagta ttgaaggtga tattgaaacc
    33961 ttggaaaaac gtatcgctgc tattgaagag gaaatgcagg ctaacggctc tgactttggt
    34021 aagctggcta ctctccaaaa agaattggat gagaaaaatg aagcactcct tgaaaaatac
    34081 gaacgctatg agtatctcag tgaatttgat agttaatact attcgaaaat caaagtgtaa
    34141 actatgtcgg cttcacattg ccgactcaag tacagcctgt ggccggcttc ctagtttgca
    34201 ctttgatttt tattgagtat aaaagaatag aaaaatccca tctcatgaac ggatttctct
    34261 attctttttt ggtttcttga tgaaagatat tttgccaggt ttcgtggcga cgccagatac
    34321 tggtatggat gataccatct aactcatagc agatgagttt ggttttttga ctgatggaag
    34381 taatttgaat gtccttgata gcagaattta actctttttt ggtcttataa gcctctttat
    34441 ccatctgctc tccatcttgg cgaatataga caaaatcatc agtcaagagt tcttctagtt
    34501 ggtttccttg gtcaatcaat tgttcacgca taagcaattt tttatagaca ttgtctaaaa
    34561 tttcatcctc aaggataggt gctggttcga tatggacatc ggtatcaaag acgccaaaac
    34621 gctcctccag catagactcg acctgatccg cgatttcatg gctttcaaaa acagacaagt
    34681 caggattcat ctctagtgta atatccaggt agatgttgct accgtaggtg cgacctcttt
    34741 gcgatttgac cttgctgatt ttgggaattt ccatgatagc cttttggtag tcctcgagca
    34801 ggcggtcgtc aaagccatct gaaagactaa aggaagactc gatgaagata tcataggcag
    34861 tcttcaagat aaagaaagtg atgatgatag caaccagttt atccacaatc ggataattga
    34921 aactactagc taggatggca atggcggttc caagtgaggt aacagcgtca gaaagattgt
    34981 ccttagcagc tgccttcagc gccttggagt tggatttctt actgaggcga gtattgtaga
    35041 gatagaccac aaacataatc gctgcagaaa tgattcctag agttgcacca agaggatcaa
    35101 tgaccgtttc ttcccgactg agaatctttt gaatggtatc tcttagaaca tcgaaaccga
    35161 catagaacat gatgatagaa gtaatcaagc ttgccaaatc ttcaatcttc caatgaccaa
    35221 aacggtggtc acggtctgca ggctggcgcg ccagccgaat cccgattaag agggccacat
    35281 ttccaatgat gtccgatacg ttattaaaac catcggccac caaactggat gaatgaagga
    35341 gatgaccagt tgctaatttg gctgcagaca agatcaaata ggtcgaaata ctgataatgg
    35401 ctccacgctc agctaacttg agatttgaga tagattgctt cattcaactt cctttctagt
    35461 cacatagcat tctaccatta tactggtttt caaggaaaaa ttcaaataag gcagttttta
    35521 ctctttgaaa actccccaaa atttggtata atggtactac tcaagggagt agctggcaga
    35581 aacctgtgat agtgtcgtca ttccgaattt tatgctgaaa agtatgcttt ccggccctat
    35641 cttaaacagc gagacttgtt atgattaaca ggtctctttt tgtttgtcgt gaaaagatat
    35701 gacagaaaga gagtctgttt tgcacacaat gtcaaggagg agacacatgt caaaagaaca
    35761 aaaacgccaa gcgttttata ctcagagccc tgaagaggtc ttgcagacta tggatgcgac
    35821 cgagcaaggt ttgtcatcaa gtgaggcgga aaagcgcctt gccgaatttg ggcacaatga
    35881 actcgaagaa ggcgagaaac gatcaatctt ggtcaaattc atcgagcaat ttaaggattt
    35941 gatgattatc atcctagttg cggcagcaat cttgtcagtc gtgacttctg gtggggaaga
    36001 tatcgcagat gccattatca tcctagctgt ggttatcatc aacgctgcct ttggtgttta
    36061 ccaagaagga aaagctgaag aagctatcga agccctcaaa tccatgtcta gtccagttgc
    36121 ccgcgttttt cgtgatggac acatggcaga gattgactct aaagaattgg taccaggcga
    36181 tatcgttgcc cttgaagcag gtgacgtggt accagcggac ctacgtttgc tagaagccaa
    36241 ctctcttaaa attgaagaag cagccttgac gggtgaatct gtaccagtcg aaaaagactt
    36301 gtcagtcgag cttgcgacag atgctggtat tggtgaccgt gtcaacatgg ccttccaaaa
    36361 ctcaaacgta acctatggtc gtgggatggg tgttgttgtc aatacaggta tgtacactga
    36421 agttggtcat attgctggta tgcttcaaga tgcggatgag actgatacac cactcaaaca
    36481 aaacttgaac aacctttcta aggtcttgac ctatgctatc ttggtcattg cccttgttac
    36541 ttttgtagtg ggtgtcttca ttcaagggaa aaatccactt ggtgagttgt tgacttctgt
    36601 tgcccttgcc gttgcagcca ttccagaagg acttcctgct atcgttacca tcgttctttc
    36661 tcttggtact caagttttgg ccaaacgaca ttccatcgtt cgtaagttgc cagcagttga
    36721 aacacttggt tcaactgaaa tcatcgcttc tgataagact ggtacgctga ctatgaacaa
    36781 gatgacagtc gaaaaagtct tctacgatgc ggttctacat gactcagctg atgatattga
    36841 actaggtctt gaaatgccac tacttcgttc agttgtcttg gccaatgata cgaaaatcga
    36901 tgtggaaggt aacttgattg gtgacccaac cgaaacagcc tttatccaat atgccttgga
    36961 caagggctat gatgtcaaag gtttcttaga gaaatatcct cgtgtagctg aattgccatt
    37021 tgactctgac cgtaagctca tgtcaacagt tcacccattg ccagatggtc gtttccttgt
    37081 agcagtcaag ggtgcgccag accaactctt aaaacgttgt cttcttcgtg ataaggctgg
    37141 ggatattgct ccgattgatg agaaggttac aaatctcatt catacaaaca attctgaaat
    37201 ggctcatcaa gccttgcgtg tccttgcagg tgcttataag attatcgata gtattccaga
    37261 aaatctcact tctgaagagc ttgaaaatga tttaattttt actggtttga ttgggatgat
    37321 tgaccctgaa cgtcctgaag ccgctgaggc tgttcgtgtg gctaaggaag cgggaatccg
    37381 tccaattatg attacaggtg accaccaaga cacagcggaa gccattgcca aacgtttggg
    37441 aatcattgac gcaaacgata cagaaggtca cgttttaact ggtgctgaac tcaatgaact
    37501 gtcagatgaa gaatttgaaa aagtcgttgg tcaatactct gtttatgccc gtgtgtctcc
    37561 agaacacaag gttcgtatcg tcaaggcttg gcaaaaacaa ggtaaagtcg ttgccatgac
    37621 aggtgacggt gtcaatgacg cgccagctct gaaaacagcc gatatcggta tcggtatggg
    37681 aatcactggt acagaggttt ctaagggggc ttctgatatg attcttgcag atgataactt
    37741 tgcgactatt atcgtcgcag tggaagaagg acgtaaggtc ttctcaaaca ttcaaaagac
    37801 tattcagtac ctactttctg ctaatactgc tgaagtatta accatcttcc tatcaacctt
    37861 gtttggttgg gatgtcttac agccggttca tcttttgtgg atcaacttgg taacggatac
    37921 cttcccagct atcgctcttg gtgttgaacc tgcagaacct ggtgtcatga atcataaacc
    37981 acgtggacgc aaggcaagct tcttctcagg tggtgttttg agttctatca tttatcaagg
    38041 tgtactccaa gcagctcttg ttatgagtgt ttatggcctt gcgattgctt acccagttca
    38101 tgtgggtgac aatcatgcta ttcatgcaga tgccctaacg atggcctttg caacccttgg
    38161 tttgattcag ctcttccatg cctacaatgt caagtctgtc taccaatcca tcttgacagt
    38221 tggcccattc aagtctaaga cctttaactg gtccatcttg gtatccttta tccttctcat
    38281 ggcaacaatc gttgtagaac cgcttgaagg aatcttccac gtaaccaaac tagacttgtc
    38341 acaatgggga attgttatgg ctggaagctt ctcaatgatt atcatcgtcg aaatcgttaa
    38401 gtttattcaa cgcaaactcg gttttgacaa gaatgcgatt taagggaggt ttcggatgtc
    38461 agcatatcaa ttaccgaccg tatggcagga tgaagctagt aatcaaggag cttttacggg
    38521 gctaaacaga ccaacagcag gtgcccgttt cgaacaaaac ttgccaaaag gagaacaagc
    38581 ttttcagctt tattcactgg gaacaccaaa tggtgtgaag gttactatct tattggaaga
    38641 attactagaa gctggtttta aggaagcggc ttacgacttg tataagattg ctatcatgga
    38701 tggggatcaa ttcggctcag actttgtgaa gctcaatcca aattccaaga ttccagcctt
    38761 attggaccag tcaggtactg aaaacgtaag agtctttgag tctgctcata ttcttcttta
    38821 ccttgctgag aaatttggag cctttttacc aagtaatcct gtggaaaagg tagaagtttt
    38881 gaattggcta ttctggcaag caggtgcagc accttttcta ggtgggggat ttggacattt
    38941 cttcaattat gctcctgaaa aattggaata tcctattaac cgttttacga tggaagtgaa
    39001 acgccagttg gatttattgg ataaggaatt ggctcagaaa ccttatattg caggcaatga
    39061 ctatacgatt gcagatattg ctatctggtc ttggtatgga cagttagttc aaggaaatct
    39121 ttaccaaggt tctgcaaaat tcttggatgc ctcaagttat caaaatctag taaaatgggc
    39181 agaaaaaatt gccaatcgtc cagctgttaa gcgtggcttg gaagtaactt atacagaaat
    39241 taaatagcaa aaaagtcatc ttcggatggc tttttgctac agttgaggga aagggtttgc
    39301 agttatcggc ttttgtgcta taattgctat aatatagtaa aaagccagag gagtgtgagg
    39361 aagtggaaaa gaaaattgtg aaggatatct tatttttatc tcaagtgtct cagccggcaa
    39421 gtcaggagga cctttatctt gccagagatt tgcaggatac actcttagca aatcgtgata
    39481 cctgtgttgg tctagctgcc aatatgattg gggtgcagaa gcgcgtgatt atctttaatc
    39541 ttggcttagt tcccgtggtc atgtttaacc cagtgcttct gtcctttgaa ggatcttatg
    39601 aggcagaaga aggctgtttg tccttggtag gtgtgagatc aactaagcgt tatgaaacca
    39661 taaggcttgc ctatcgtgac agcaagtggc aggaacagac cattaccttg acaggcttcc
    39721 cagctcagat ttgccagcat gagctggatc acttggaagg acgaatcatt taggaggaaa
    39781 gcaaatgaaa cgaatagtct ttgaacttat ttttatcgca acgacctggt atatcttttt
    39841 accgcccctt aacctgacca gctgggaatt tctcttcttc ctctgtgggc atttgttagt
    39901 tgtggcaatc ttatttggct ttggcaaggg gataaacctt gtcaaaacgg ttcatgtgcg
    39961 ccacggtaag gcggaagctg ccttaaatct tgagggtttc aaaatcaatc ggttagggaa
    40021 aattctgtta gcttcgattg gaggaattct tctcttggca gctttggttt ccttggtaac
    40081 ttccagcatg tttcaggcta aaaattatgc caatgtagtc acggttacgg aaaaagactt
    40141 tactgaattt cctaagagtg acaccagtaa ggttcctatc ctagatagaa gtactgctga
    40201 aaaaattgga gaccgctact tgggttccct aaccgataag gtgtcgcaat acgtagcggc
    40261 agatacctat acccaattga caattgatgg gaaaccttat cgggtcacac cactagaata
    40321 tgcagaccct atcaaatggt ttaacaatca agccaaggga atcggtgagt atatcaaggt
    40381 ggacatggta actggaaatg cggatttggt ggacttgaag acaccaatca agtattcaga
    40441 ctcggagtat tttaaccgtg atgtcaaacg tcacctgcgc ttgaagtacc cgaccaaaat
    40501 ctttaaaact ccatcttttg aggtggacga tgagggcaat cctttctatg tagcaacggt
    40561 ttaccaaaag caatttggac ttgcggttcc tcgtcctgct tcagtcatta tcttggatgc
    40621 tacaaatgga gaaaccaagg aatacagctt atcagatgtt ccagaatggg tggataggat
    40681 ctatccagca gaggaaacca ttgagcaaat caactacaac ggcaagtaca aggacggttt
    40741 cttgaatgcc atgatttcca agaaaaacgt gacccagact accaatggct ataattactt
    40801 gtctatcggt aatgacatct atctctacac aggtgtgacg tcggctaatg cggatgagag
    40861 taatcttggt ttcatccttg aaaatatgcg aacaggagaa atcactaagt atagcttggc
    40921 ttctgcgaca gaagaatcag cccgtgaatc agcagaaggt gctgttcagg agaaatccta
    40981 caaagcaacc ttcccaatcc tcatcaacct caatgacaag cctctctaca tcatgggctt
    41041 aaaggacaat gctggcttgg tcaaagagta cgccctggta gacgcagtcg agtaccaaaa
    41101 tgttatcgtt gctactacag tggaagagat gctcagcaag tatgccaata aaaacgacct
    41161 tgaaattgac aatgcaacga cagaaagcat caagggagta gtagcagacc tcaaatcagc
    41221 tgttatcaag ggagacactg tctacttctt taaagttgat ggcaagatat acaaggtcaa
    41281 ggcttcagta tccgatgacc ttccttacct tgaaaatggt aaaaccttcg aaggtcaagt
    41341 aggaaaagac aattatctca agacctttaa agtacaataa tactcttcga aaatctctag
    41401 ctctatctgc aacctcaaaa cagtgttttg agcagcctgc ggctagtttc ctag
//
LOCUS       DAVKAO010000011        37021 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKAO010000011 DAVKAO010000000
VERSION     DAVKAO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.011"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            162..1187
                     /gene="trpS"
                     /locus_tag="VWX16_000607"
     CDS             162..1187
                     /gene="trpS"
                     /locus_tag="VWX16_000607"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9157999.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSGVKGA
                     MGLHYFN"
     gene            1339..2817
                     /gene="guaB"
                     /locus_tag="VWX16_000608"
     CDS             1339..2817
                     /gene="guaB"
                     /locus_tag="VWX16_000608"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9158000.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDIVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2868..3965)
                     /gene="recF"
                     /locus_tag="VWX16_000609"
     CDS             complement(2868..3965)
                     /gene="recF"
                     /locus_tag="VWX16_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9158001.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(3968..4336)
                     /gene="yaaA"
                     /locus_tag="VWX16_000610"
     CDS             complement(3968..4336)
                     /gene="yaaA"
                     /locus_tag="VWX16_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9158002.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4495..5745
                     /locus_tag="VWX16_000611"
     CDS             4495..5745
                     /locus_tag="VWX16_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9158003.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKKTLFSPVVVDNGFDSALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYPSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5742..7025
                     /locus_tag="VWX16_000612"
     CDS             5742..7025
                     /locus_tag="VWX16_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9158004.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDMKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7067..7888
                     /locus_tag="VWX16_000613"
     CDS             7067..7888
                     /locus_tag="VWX16_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9158005.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7899..8444
                     /gene="pgsA"
                     /locus_tag="VWX16_000614"
     CDS             7899..8444
                     /gene="pgsA"
                     /locus_tag="VWX16_000614"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9158006.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFLFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8441..9268
                     /locus_tag="VWX16_000615"
     CDS             8441..9268
                     /locus_tag="VWX16_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9158007.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9253..10092
                     /locus_tag="VWX16_000616"
     CDS             9253..10092
                     /locus_tag="VWX16_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9158008.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPNQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10085..10879
                     /locus_tag="VWX16_000617"
     CDS             10085..10879
                     /locus_tag="VWX16_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9158009.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10938..11756
                     /gene="mreC"
                     /locus_tag="VWX16_000618"
     CDS             10938..11756
                     /gene="mreC"
                     /locus_tag="VWX16_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9158010.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11756..12250
                     /gene="mreD"
                     /locus_tag="VWX16_000619"
     CDS             11756..12250
                     /gene="mreD"
                     /locus_tag="VWX16_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9158011.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12344..13522
                     /locus_tag="VWX16_000620"
     CDS             12344..13522
                     /locus_tag="VWX16_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9158012.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAERATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13746..14525
                     /gene="rpsB"
                     /locus_tag="VWX16_000621"
     CDS             13746..14525
                     /gene="rpsB"
                     /locus_tag="VWX16_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9158013.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14604..15644
                     /locus_tag="VWX16_000622"
     CDS             14604..15644
                     /locus_tag="VWX16_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9158014.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15890..16213
                     /gene="tnpB"
                     /locus_tag="VWX16_000623"
     CDS             15890..16213
                     /gene="tnpB"
                     /locus_tag="VWX16_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9158015.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKGFS
                     ITPKI"
     gene            complement(16368..16655)
                     /locus_tag="VWX16_000624"
                     /pseudo
     CDS             complement(16368..16655)
                     /locus_tag="VWX16_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16646..17044
                     /locus_tag="VWX16_000625"
     CDS             16646..17044
                     /locus_tag="VWX16_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9158016.1"
                     /translation="MKAPIPKAPLAHSFGSASIIAHTIHQKFNLKVPNYRQEEDWTKM
                     GLPITRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(17241..18173)
                     /gene="cysK"
                     /locus_tag="VWX16_000626"
     CDS             complement(17241..18173)
                     /gene="cysK"
                     /locus_tag="VWX16_000626"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9158017.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKSGEK
                     PGPHKIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIY
                     GAIEVAKKLGTGKKVLVLAPDNGERYLSTALYEL"
     gene            complement(18458..19093)
                     /locus_tag="VWX16_000627"
     CDS             complement(18458..19093)
                     /locus_tag="VWX16_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9158018.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            19149..20447
                     /locus_tag="VWX16_000628"
     CDS             19149..20447
                     /locus_tag="VWX16_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9158019.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            20444..21106
                     /locus_tag="VWX16_000629"
     CDS             20444..21106
                     /locus_tag="VWX16_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9158020.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            21186..21734
                     /gene="raiA"
                     /locus_tag="VWX16_000630"
     CDS             21186..21734
                     /gene="raiA"
                     /locus_tag="VWX16_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9158021.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            21870..23843
                     /locus_tag="VWX16_000631"
     CDS             21870..23843
                     /locus_tag="VWX16_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9158022.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            23840..24292
                     /gene="rplI"
                     /locus_tag="VWX16_000632"
     CDS             23840..24292
                     /gene="rplI"
                     /locus_tag="VWX16_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9158023.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            24336..25688
                     /gene="dnaB"
                     /locus_tag="VWX16_000633"
     CDS             24336..25688
                     /gene="dnaB"
                     /locus_tag="VWX16_000633"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9158024.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            25690..25956
                     /locus_tag="VWX16_000634"
     CDS             25690..25956
                     /locus_tag="VWX16_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158025.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            26045..27163
                     /gene="cbpD"
                     /locus_tag="VWX16_000635"
     CDS             26045..27163
                     /gene="cbpD"
                     /locus_tag="VWX16_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9158026.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRSRREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNYQNQSGNISSYGSNNSSTV
                     GWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQTGWLKENGSWYYLGSSGAM
                     KTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      27246..27348
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27419..27709
                     /locus_tag="VWX16_000636"
     CDS             27419..27709
                     /locus_tag="VWX16_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9158027.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQGADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            27672..28430
                     /locus_tag="VWX16_000637"
     CDS             27672..28430
                     /locus_tag="VWX16_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158028.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            28469..29476
                     /locus_tag="VWX16_000638"
     CDS             28469..29476
                     /locus_tag="VWX16_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9158029.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            29476..30204
                     /locus_tag="VWX16_000639"
     CDS             29476..30204
                     /locus_tag="VWX16_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158030.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDN"
     gene            30323..30781
                     /locus_tag="VWX16_000640"
     CDS             30323..30781
                     /locus_tag="VWX16_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9158031.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            30783..33215
                     /locus_tag="VWX16_000641"
     CDS             30783..33215
                     /locus_tag="VWX16_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9158032.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQILFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDEESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            33869..34522
                     /gene="rr06"
                     /locus_tag="VWX16_000642"
     CDS             33869..34522
                     /gene="rr06"
                     /locus_tag="VWX16_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9158033.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            34519..35850
                     /gene="hk06"
                     /locus_tag="VWX16_000643"
     CDS             34519..35850
                     /gene="hk06"
                     /locus_tag="VWX16_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9158034.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCTPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            35933..36460
                     /locus_tag="VWX16_000644"
     CDS             35933..36460
                     /locus_tag="VWX16_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9158035.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            36539..>37021
                     /locus_tag="VWX16_000645"
     CDS             36539..>37021
                     /locus_tag="VWX16_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEU9158036.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVAVASLVMGSVVHATENEGST
                     QAATFSNMANKSQTEQGEINIERDKAKTAVSEYKEKKVSEIYTKLERDRHKDTVDLVN
                     KLQEIKNEYLNKIVESTSKIEIQGLITTSRSELDEAVSKYKKAPSSSSSSGSSTKPET
                     P"
BASE COUNT        11520 a         6020 c         8133 g        11348 t
ORIGIN      
        1 acagaaatat tcaaattttt atttgtcaat tttgtgtaaa ttatatttac agtatccttt
       61 acacaaatct gtaaaaagca aggctgattt attttgataa attacggtta tttcattaaa
      121 aaaatgctat aattgaaagg actatatcga aggagaacaa aatgactaaa cccattattt
      181 taacaggaga ccgtccaaca ggaaaattgc atattggaca ttatgttgga agtctcaaaa
      241 atcgagtatt attacaggaa gaggataagt atgatatgtt tgtgttcttg gctgaccaac
      301 aagctttgac agatcatgcc aaagatcctc aaaccattgt agagtctatc ggaaatgtgg
      361 ctttggatta tcttgcagtt ggattggatc caaataagtc aactattttt attcaaagcc
      421 agattccaga gttggctgag ttgtctatgt attatatgaa tctagtttcg ttagcacgtt
      481 tggagcgaaa tccaacagtc aagacagaga tttctcagaa aggatttgga gaaagcattc
      541 cgacaggatt cttggtctat ccaatcgctc aagcagctga tatcacagct ttcaaggcta
      601 attatgttcc tgttgggaca gatcagaaac caatgattga gcaaactcgt gaaattgttc
      661 gttcttttaa caatgcatat aactgtgatg tcttggtaga gccggaaggt atttatccag
      721 aaaatgagag agcagggcgt ttgcctggtt tagatggaaa tgctaaaatg tctaaatcac
      781 taaataatgg tatttattta gctgatgatg cggatacttt gcgtaaaaaa gtaatgagta
      841 tgtatacaga tccagatcat atccgcgttg aggatccagg taagattgag ggaaatatgg
      901 ttttccatta tctagatgtt tttggtcgtc cagaagatgc tcaagaaatt gctgatatga
      961 aagaacgtta tcaacgaggt ggtcttggtg atgtgaagac caagcgttat ctacttgaaa
     1021 tattagaacg tgaactgggt cctattcgtg agcgccgtat tgaatttgct aaggatatgg
     1081 gagaagttta taatatgatt caaaaaggta gtgaaagagc gcgtgaagtt gcgggtcaaa
     1141 ccctatctgg ggtaaaaggg gcaatgggac ttcattactt taactaagtt tattttacaa
     1201 gaaactatct tttctatctc gaagaaaaga tagtttttta tttacccctg taacatttga
     1261 caaattttta tagaatggta tgatagatac aatataaaaa gagtcaagct caaaaagaaa
     1321 gaaaagagga aacttcgaat gtctaattgg gacactaaat ttttgaaaaa aggttttacc
     1381 tttgatgatg tattgcttat tccagctgaa agtcatgtgt tgcctaacga tgcagattta
     1441 acaactaaat tggcagataa tttgacttta aatatcccaa ttattaccgc tgccatggac
     1501 acagttacag agagtcaaat ggccattgct attgctcgtg caggcggtct cggagttatc
     1561 cataaaaaca tgtcaattgc tcaacaagca gacgaggttc gtaaggtaaa acgttctgaa
     1621 aatggagtta ttattgatcc gttcttcttg acgcctgaac atacaattgc tgaagcagat
     1681 gagcttatgg gtcgttaccg catcagtggt gttccagttg ttgaaacact tgaaaatcgt
     1741 aaattggttg gtattttgac aaaccgagat cttcgtttta tttcagatta taatcaacca
     1801 atttcaaacc atatgactag tgaaaatctt gttactgctc ctgtgggtac ggatcttgca
     1861 acggctgaga gtattcttca agagcatcgt attgaaaaac ttccgttggt cgatgaagaa
     1921 ggcagtcttt ctggtttgat cactatcaaa gatattgaaa aagttattga gtttccaaat
     1981 gcggctaaag atgagtttgg tcgtcttcta gttgcaggtg cagtaggtgt tacttcagat
     2041 acatttgaac gtgcagaggc tctttttgag gcaggagcgg atgcgattgt tattgatact
     2101 gcacatggtc attctgcagg tgtcttgcgt aaaattgccg agattcgtgc tcatttccca
     2161 gatcggactt tgattgctgg aaatattgct actgctgaag gtgcacgtgc cctttatgaa
     2221 gcgggtgtag acattgttaa ggttggtatt ggaccaggtt ctatctgtac tactcgtgtg
     2281 attgctggtg ttggtgttcc gcaagtaaca gctatctacg atgctgcagc tgttgcgcgc
     2341 gaatatggta aaacgattat tgctgacggt gggatcaagt attctggaga tattgtaaaa
     2401 gcacttgctg caggtggaaa tgctgttatg cttggatcta tgtttgctgg aactgatgaa
     2461 gctccaggcg aaactgaaat cttccaagga cgtaaattca agacttaccg tggtatggga
     2521 tcaattgctg ctatgaagaa aggttcaagc gaccgttatt tccaaggttc tgtcaatgaa
     2581 gcaaacaagc tcgttccaga aggaattgaa ggtcgtgttg cttataaagg agcggcagct
     2641 gatattgttt tccaaatgat tggtggtatt cgctctggta tgggttactg tggtgcagct
     2701 aaccttaaag aactacacga taatgctcaa tttattgaaa tgtctggtgc tggtttgaaa
     2761 gaaagccatc ctcatgatgt gcaaattact aatgaggcac caaattattc tatgtaaaaa
     2821 acaatgaaaa gaactccagt gaaaacagga gttcttttac aatgttgtca atttccattt
     2881 acagcagctt taccatcctg aatagtgaag atacttagat tttctggcag attttgaaga
     2941 tggtctaagc ttgttgttgt gataaaggtt tggattgatt gagaaatcgt ttctaataat
     3001 tttaactgtc tagtgttgtc aagttcactc atcacatcgt caagcagtaa tatcggagat
     3061 tctgtggtaa tgctttccat taattcgatt tctgctaatt ttatcgagag gacgagacta
     3121 cgatgttgac cttggcttcc gaaactagca tccatcccat ttatataaaa agaaatgtca
     3181 tctcgatgag gaccgacacc agtattcttt ttaaataaat ctctggatct acttttttct
     3241 aaagcaattt tgaaagattc ggataagttt tgtttgtcag ttatattgac agaagattga
     3301 taggatattg acaactcttc gatctgatta gagagttcaa aatgtttctt acgcccaaat
     3361 gattctagtt tttttatgaa atctaagcgg tgattcatta cacgacatcc ataatcaact
     3421 agctgatcat ctaacacaga aaggaatgtt tcatctattt tttgagctga ttttaggtaa
     3481 gtgtttcttt gctttaggat gtggttataa ttggttaagt cagataaata gattggctta
     3541 atttgcccaa gttccatatc aatgaatttt cgtcgaatcg aaggtgctcc tttaattagt
     3601 tgtaaatctt caggagcaaa taagacaaca ttcatgtgtc ctacataatc tgaaaggcgt
     3661 gcctgtttta agtgattaac ttttgttaca cggccttttt gtgttagttc gatttctagg
     3721 ggaatggatc cagttttttt ctgaacgaga cctgaaagat gaagttgttc ctcatcaaaa
     3781 tgaatgagat ttttatctgt tcgagttcga tgactacgcg ttaaggctaa aaaatagata
     3841 gcctctaaca tgtttgtttt tccttgtgca ttacgtccca aaaagacatt caatttagga
     3901 ttaaagtcta ttttcgtttc tttgtaatta cgaaaagtct tgagagatag atgttgtagc
     3961 cacatgatta tctaccaggg aatcgtggag cttgttggct tttaggtgat gaagcaggtt
     4021 tcgatttgtc ttttttaact cccttattca tctttttgac aagtttagcg atccgttctt
     4081 tttcaacttt atcagcttgg tattcatctt gttcttcaga agtaggttgt gtcaacaaga
     4141 tgtcaatgtt catgtcaggg atgtcaactt tatcgccaat acgaagtttt tttccacgac
     4201 gactctctaa ttccctatta aagtaaacag aatgttcaga gagaaatgat ttaatagctc
     4261 ctccgctatg tgtaattcca agttctttga gtagtgcttg gagggtaata aattcttcaa
     4321 ataatttgta ttccataatt cacctcaatc tttggtatta taccatattt tcctaaaaat
     4381 agtgagagag acagctagaa atgtaagcga tttttatttc aaaagtgtta cagggaacaa
     4441 gaaaatttca ggttttcgtg atataataga agtctgtata taaggaggta aatcatggag
     4501 ttagtgcatg gaatttcaac acattttatc caatcaaaaa agtttaaaac aaacaaaatt
     4561 accgtgcgtt ttaccgctcc attatccctt gatacgattg caggtcacat gttgagtgca
     4621 agtatgctag agactgctaa tcagatgtac cccacttctc aagatttgag gagacacttg
     4681 gccagtctat acggtacaga tatgtcaacc aattgtttca gaagagggca aagccacatt
     4741 atagaattga catttaccta tgttcgtgat gagtttttaa gtaggaaaaa cgtgctaacc
     4801 tctcagattt tggaacttgt aaaaaaaact cttttttcac ccgtagtagt tgataatggg
     4861 tttgattcgg ccttatttga aattgagaaa aaacaattgc tagcaagttt agcagctgat
     4921 atggatgatt ctttttattt tgcacataaa gaattggata aattgttttt tcatgatgaa
     4981 cgtcttcaat tggaatatag tgatttacga aatcgtattt tagctgaaac tccacaaagt
     5041 tcttactctt gtttccaaga atttttagcc aatgatcgaa tagatttctt tttcctaggt
     5101 gattttaatg aggttgaaat tcaaaatgta ttagaatcat ttggctttaa aggtcgaaaa
     5161 ggagatgtga aggttcagta ttgtcaacct tattctaata tccttcagga aggtatggtt
     5221 cggaaaaatg tgggacaatc cattttggaa ttaggttatc attacccttc taaatatggt
     5281 gatgaacaac atttacccat gattgtaatg aatggtttac ttggtggatt tgctcactct
     5341 aagctcttta caaatgtccg tgaaaatgct ggattagctt ataccatttc aagtgagctt
     5401 gatttattta gtggattctt gaggatgtat gctggtatca atcgagaaaa tcgtaaccag
     5461 gctcgtaaaa tgatgaataa tcaactgctt gatttaaaaa aaggttattt tacagagttt
     5521 gagttaaatc agaccaagga aatgattcgt tggtcgttgt tactttctca agataatcaa
     5581 tcttcattga ttgaacgtgc ttatcaaaat gccttatttg gaaaatcttc agcagacttt
     5641 aaaagttgga ttgcaaagct tgaacaaatt gacaaagatg ctatttgtag agtagctaat
     5701 aatgtgaaac tacaagcgat ttactttatg gaaggaatag aatgacaaag gttgtttttg
     5761 aagaaaaata ctatccagct gtaaaagaaa tggtttatcg aactcgtttg gccaacggat
     5821 tgacagttgc tcttttgcct aaaaaggaat ttaaagaggt ttacgggagt gtcactgtac
     5881 agtttggttc ggtagatacg tttgtcacag aagttgacgg agatgtaaaa caatatcctg
     5941 gaggaattgc tcattttctt gaacataaat tatttgagag agaagattct agtgatttga
     6001 tgtcggcttt tacgagtcta ggtgcagata gtaatgcctt tacaagcttt acaaaaacaa
     6061 actatctttt ttcagcaacg gattattttt tagaaaattt agatttactt gatgaattgg
     6121 taacatcagc acactttact gaagcttcca ttctgacaga gcaggatatt attcagcaag
     6181 aacgagaaat gtaccaagat gatccagatt cgtgtttatt cttttcaact ttagcgaatt
     6241 tgtatcctgg tacaccttta gcaactgata tagttggaag tgaggagtcc atttcccaaa
     6301 tcaatctaac taatttgcaa gaaaatttta caaagtttta caaacctgta aacatgtctc
     6361 tgtttttagt tggtaatttt gatgtggagc gagtacagga ctattttgaa agcaaagaac
     6421 tgaaagattc agattttcag gaagtagcaa gagaaaagtt gtttttacag cctgtaaagc
     6481 caacagatag tatgagaatg gaagtatctt ctcccaaact agcgattgga gttagaggta
     6541 agcgagaagt ttctgaagcg gattgctatc gacatcatat tttattaaaa ttattgtttg
     6601 caatgatgtt tggttggact tcggatcgtt ttcaaaaatg ttatgaatca ggtaaaattg
     6661 atgcatcctt atctctggaa gttgaagtaa caagtcgctt tcattttgtc atgttgacaa
     6721 tggatatgaa agagccagtt gctttgtctc atcaatttag gaaggctatt cgtaatttta
     6781 caaaggattt agatattaca gaggaacatt tagatattat caaaagagag atgtttggcg
     6841 aatttttcag tagcatgaac tctcttgaat ttattgcaac gcaatatgat gcttttgaaa
     6901 atggtgagac aatttttgat ttgccgaaaa ttttacagga aattacttta gaggatgtcc
     6961 ttgatgctgg acatcattta atagatgatg gtgacatagt tgattttaca atattcccat
     7021 cgtagtaacc tattataata gacactagaa agaagggatg acaagtatga gaaaaaaaac
     7081 aattggagag gttttacgat tagctagaat caatcaggga ttgagtttag atgaattgca
     7141 gaaaaagaca gaaatccagt tagatatgtt ggaagcaatg gaagcagacg atttcgatca
     7201 acttccaagt cctttttaca cgcgttcttt cttgaaaaaa tatgcatggg ctgttgagtt
     7261 agatgaccaa attgttttgg atgcttatga ttctgggagt atgattactt atgaggaagt
     7321 agatgttgat gaagatgagt tgacaggtcg tagacgttca agtaagaaaa agaagaaaaa
     7381 aacatcattt ttacctttat tttattttat cctttttgct ttatcgattt taatttttgt
     7441 gacttattat gtttggaact atattcaaac tcaaccagag gagccttctc tttctaatta
     7501 cagtgtggtt caatcaacaa gttcaactag ctctgttccc cactcctcaa gtagtagttc
     7561 ttctagtata gaatcagcta taagtgtatc aggcgaagga aatcatgtag aaatcgctta
     7621 taagacaagt aaggaaacag ttaaattgca attggcagtt tcagatgtta caagttgggt
     7681 cagtgtttca gaaagcgaac ttgagggcgg tgtaacctta tcgccaaaga agaaaagtgc
     7741 agaagcaaca gttgcaacta aaagtcctgt aacaattacg ttaggtgttg taaaaggtgt
     7801 tgatttgaca gtagataatc agactgttga tttatcgaaa ttaacagctc agactggaca
     7861 aatcactgta acctttacta aaaattaagg aaaaacgaat gaaaaaagaa caaattccca
     7921 atctcttaac aataggtcga attctcttta tacctatttt tctctttatt ttaacgatag
     7981 gaaattcgat agagagtcat atagttgcag ctattatctt tgctgttgcc agtattaccg
     8041 actatttaga tggatattta gctcgtaaat ggaatgtggt cagtaatttt ggtaaatttg
     8101 cagatcctat ggcggataag ttactagtta tgtcggcttt tattatgttg attgagttag
     8161 gtatggctcc ggcttggatt gttgcagtga ttatctgtcg tgagttagct gtgacaggtt
     8221 taaggctttt attggttgaa actggtggaa caattttagc agcagcaatg cctggaaaaa
     8281 ttaaaacttt tagtcagatg tttgccatta ttttcttgct attacattgg actttgcttg
     8341 gtcaagttct actttatgta gccttatttt tcactatcta ctctggctat gactatttca
     8401 agggtagtgc ctatgtattt aaagggacat ttggttcgaa atgaaatcaa taattgatgt
     8461 aaaaaatctt tcttttcgct ataaagaaaa tcagaactac tacgatgtga aggatattac
     8521 gtttcacgtg aaacgtggag aatggctttc gattgtaggg cataatggta gtggtaaatc
     8581 aacgacggtt cgattaattg atggcttact ggaagcagaa tccggagaga ttgtaattga
     8641 tggccaacgt ttgactgagg aaaatgtttg gaatatacgt cgtcaaatcg gtatggtttt
     8701 tcaaaatcca gacaatcaat ttgttggagc gactgttgaa gatgatgttg cttttggttt
     8761 ggaaaatcag ggactttctc gtcaagaaat gaaaaagaga gtggaagaag ctctggcttt
     8821 agttggcatg ttggacttta aaaagagaga gccagcgcgt ctatcaggtg gccaaaagca
     8881 acgtgtggcc attgcaggtg ttgtagccct aagaccagct attttaatct tagatgaagc
     8941 aacgagtatg ttggatcctg aggggcgtag agaacttatt gggacagtaa aaggaattcg
     9001 aaaagactat gatatgacgg tcatttctat tacccatgat ttggaagaag tcgccatgag
     9061 tgatcgcgta ttggtcatga aaaaagggga aattgaatca actagtagtc caagggagct
     9121 tttctctcga aatgatttag atcaaattgg attagacgat ccttttgcta atcaattaaa
     9181 aaaatctttg agccagaatg gctatgattt acctgaaaat tatttgacag aaagtgagct
     9241 agaggataag ctatgggaat tgctctagaa aatgtgaatt ttacatatca agaaggtact
     9301 cccttagctt cagcagcttt gtcggatgtt tctttgacga ttgaagatgg ctcttataca
     9361 gctttaattg ggcacacagg tagtggtaaa tcaactattt tacaactctt aaatggttta
     9421 ttggtgccaa atcaagggag tgtgagggtt tttgatacct taatcacctc gacttctaaa
     9481 aataaagata ttcgtcaaat tagaaaacag gttggcttgg tatttcagtt tgctgaaaat
     9541 cagatttttg aagaaacggt tttgaaggac gttgcttttg gaccgcaaaa ttttggagtt
     9601 tctgaagaag atgctgtgaa gactgcgcgt gagaaactgg ctctggttgg aattgatgaa
     9661 tcactttttg atcgtagtcc gtttgagctg tcagggggac aaatgagacg tgttgccatt
     9721 gcaggcatac ttgccatgga gccagctata ttagtcttag atgagccaac agctggtcta
     9781 gatcctctag ggagaaaaga gttgatgacc ctgttcaaaa aactccacca gtcagggatg
     9841 accatcgtct tggtaacgca tttgatggat gatgttgctg aatatgcaaa tcaagtctat
     9901 gtaatggaaa agggacgttt agtaaagggg ggcaaaccaa gtgatgtctt tcaagacgtt
     9961 gtttttatgg aagaagttca gttgggagta cctaaaatta cggccttttg taaacgattg
    10021 gctgatagag gcgtgtcatt taaacgatta ccgattaaga tagaggagtt caaggagtcg
    10081 ctaaatggat agtatgattt tggggcgtta tatcccaggg gattcgattg ttcaccgatt
    10141 ggatccacgt agcaaattgc ttgctatgat gctactgatt ttgattgtgt tttgggctaa
    10201 taaccccttg acgaatctga ttctttttat agcgacaggg atatttattg ccttgtcagg
    10261 agtatctctt tcatttttta ttcagggctt gaagtctatg tttttcttga ttgccttcac
    10321 aactattttt caactatttt tcatttctaa tgggaatgtt ttatttgagt tttcgtttgt
    10381 gagaatcacg gattatgctt tgcaacaagc tgggattatt ttttgtcgct ttgtattgat
    10441 tattttcttt tcaactttgt taaccttaac aaccatgcct ttaagtttgg catcagctgt
    10501 cgaagcttta ttagcacctt taaagcgtgt gaaagttcca gttcatgaaa ttggattgat
    10561 gctgtctatg agtttgcgtt ttgtcccaac cttgatggat gatacgactc ggattatgaa
    10621 tgcacagaaa gctcgtggag tggattttgg agaaggaagc atcgttcaaa aagtaaaggc
    10681 gatgattccc attttgattc ctctttttgc gacaagttta aaacgtgcag attccttggc
    10741 tatcgctatg gaagcgcgtg gctatcaggg tggaaaaggc agaagtcaat accgacaatt
    10801 gaaatggact ctaaaggata cgctgaccat tcttgttatt ctcgtacttg gttgttgttt
    10861 atttttctta aaatcttagt agctttcagg aattgataaa aagttactgt aacagttttt
    10921 gatataaagg tagggatatg aaccgtttta aaaaatcaaa atatgtcatt attgtttttg
    10981 tcactgttct gcttgtgtca gctctcttag cgacgactta ttcaagtaca attgtgacaa
    11041 aattaggaga tggaatctca ttggttgata gagttgtaca aaaacctttt cagtggtttg
    11101 attctgtcaa atcagatttg gctcatttga cacgaacata taatgaaaat gaaagtttga
    11161 agaaacagct ttaccaatta gaagttaaat caaatgaggt ggaaagttta aagacagaaa
    11221 atgaacaact gcgccaattg cttgatatga agtctaaatt gcaagccaca aagactttag
    11281 cagcagatgt tattatgcgt tctccggtat cttggaagca ggagttgacc ttagatgcag
    11341 gtagatcaaa aggtgcttct gagaacatgt tagctattgc aaatggtggc ttgattggga
    11401 gtgtttcaaa agtagaggag aactctacta tagtcaacct tctgacaaat acggaaaatg
    11461 ctgataagat ttctgttaaa attcaacatg gctctactac aatttatgga attattattg
    11521 gctatgacaa ggaaaatgac gttcttaaaa ttagccaatt aaatagtaat agcgatatta
    11581 gtgcgggaga taaggtgact actggtggat taggaaactt taacgttgct gatattcctg
    11641 ttggtgaagt ggttgccaca acgcatagta cagactattt gacacgagaa gtaactgtta
    11701 aattgagtgc agatactcat aatgtagatg tgatagaatt agtggggaat tcataatgag
    11761 acagttgaag cgagttggag tatttttatt gcttcctttc tttgttctaa ttgacgccca
    11821 tattagccag cttctgggct catttttccc ccatgtacat ttggctagtc attttctttt
    11881 tctatttctc ttatttgaga cgatagaagt atcagagtat ctctacctag tctattgttt
    11941 tgttataggc ttggtttatg atgtttactt tttccatcta atagggatta caactctctt
    12001 atttatctta ttgggagcct tccttcataa attgaatagt gttattttgt tgaatcgttg
    12061 gacaagaatg ctagctatga ttgtgctgac attcctgttt gaaatgggta gttatctttt
    12121 ggcttttatg gtagggttga cagtagatag catgtcgatt tttatagtct atagcttggt
    12181 accgacgatg attttaaatt ttttatggat tactgttttt caatttattt ttgaaaaata
    12241 ttatctataa gaacgacata taaatgtaac aaaggcgtaa tatttattag gccttttttt
    12301 ggtatactag tattgtcttt aaaagaagga gtatctacgt aatatgaaga aaaaaatctt
    12361 agcgtcactt ttattaagta cagtaatggt ttctcaagta gctgttttaa caactgcgca
    12421 tgcagaaacg actgatgaca aaattgctgc tcaagataat aaaattagta acttaacagc
    12481 acaacaacaa gaagcccaaa aacaagttga ccaaattcag gagcaagtat cagctattca
    12541 agctgagcag tctaacttgc aagctgaaaa tgatagatta caagcagaat ctaagaaact
    12601 cgagggtgag attacagaac tttctaaaaa cattgtttct cgtaaccaat cgttggaaaa
    12661 acaagctcgt agtgctcaaa caaatggagc cgtaactagc tatatcaata ccattgtaaa
    12721 ctcaaaatca attacagaag ctatttcacg tgttgctgca atgagtgaaa tcgtatctgc
    12781 aaacaacaaa atgttagaac aacaaaaggc agataaaaaa gctatttctg aaaaacaagt
    12841 agcaaataat gatgctatca atactgtaat tgctaatcaa caaaaattgg ctgatgatgc
    12901 tcaagcattg actacgaaac aggcagaact aaaagctgct gaattaagtc ttgctgctga
    12961 gagagcgaca gctgaagggg aaaaagcaag tctattagag caaaaagcag cagctgaggc
    13021 agaggctcgt gcagctgcgg tagcagaagc agcttataaa gaaaaacgag ctagccaaca
    13081 acaatcagta cttgcttcag caaacactaa cttaacagct caagtgcaag cagtatctga
    13141 atctgcagca gcacctgtcc gtgcaaaagt tcgtccaaca tacagtacaa acgcttcaag
    13201 ttatccaatt ggagaatgta catggggagt aaaaacattg gcaccttggg ctggagacta
    13261 ctggggtaat ggagcacagt gggctacaag tgcagcagca gcaggtttcc gtacaggttc
    13321 aacacctcaa gttggagcaa ttgcatgttg gaatgatggt ggatatggtc acgtagcggt
    13381 tgttacagct gttgaatcaa caacacgtat ccaagtatca gaatcaaatt atgcaggtaa
    13441 tcgtacaatt ggaaatcacc gtggatggtt caatccaaca acaacttctg aaggttttgt
    13501 tacatatatt tatgcagatt aatttacaga gggactcgaa tagagccctc ttttcaggtt
    13561 ttaccgtgac aatccctatt aaaaattata tcaaaatagc ttgaaaatat tggaaaagta
    13621 tggtagaatg aaaattgtcg tgtgaacgat aatactcatt cttgatgaat tgtgaagcag
    13681 ttgcccttgg gtcgttttgc gagttgaagt caagaagagg aaaaaaacaa aaaggagaaa
    13741 tactcatggc agtaatttca atgaaacaac ttcttgaggc tggtgtacac tttggtcacc
    13801 aaactcgtcg ctggaatcct aagatggcta agtacatctt tactgaacgt aacggaatcc
    13861 acgttatcga cttgcaacaa actgtaaaat acgctgacca agcatacgac ttcatgcgtg
    13921 atgcagcagc taacgatgca gttgtattgt tcgttggtac taagaaacaa gcagctgatg
    13981 cagttgctga agaagcagta cgttcaggtc aatacttcat caaccaccgt tggttgggtg
    14041 gaactcttac aaactgggga acaatccaaa aacgtatcgc tcgtttgaaa gaaattaaac
    14101 gtatggaaga agatggaact ttcgaagttc ttcctaagaa agaagttgca cttcttaaca
    14161 aacaacgtgc gcgtcttgaa aaattcttgg gcggtatcga agatatgcct cgtatcccag
    14221 atgtgatgta cgtagttgac ccacataaag agcaaatcgc tgttaaagaa gctaaaaaat
    14281 tgggaatccc agttgtagcg atggttgaca ccaatactga tccagatgat atcgatgtaa
    14341 tcatcccagc taacgatgac gctatccgtg ctgttaaatt gatcacagct aaattggctg
    14401 acgctattat cgaaggacgt caaggtgagg atgcagtagc agttgaagca gaatttgcag
    14461 cttcagaaac tcaagcagat tcaattgaag aaatcgttga agttgtagaa ggtgacaacg
    14521 cttaatttat acaaatagta attacctagg agggcggggc ttagcccggc tctcctattt
    14581 tcaaaaaata taggagaatt aaaatggcag aaattacagc taaacttgta aaagagttgc
    14641 gtgaaaaatc tggtgccggt gttatggacg ctaaaaaagc gcttgtagaa acagacggtg
    14701 acatcgaaaa agcgattgaa ttgcttcgtg aaaaaggtat ggctaaggca gctaagaaag
    14761 ctgaccgtgt tgctgcagaa ggtttgactg gtgtttatgt taacggtaat gttgcagcag
    14821 ttattgaagt aaacgctgaa actgacttcg ttgcaaaaaa cgctcaattc gttgaattgg
    14881 taaatactac agctaaagtc attgctgaag gaaaacctgc taacaatgaa gaagctcttg
    14941 ctttgataat gccttcaggt gaaactcttg aagctgcata cgtatctgca acagcaacta
    15001 tcggagagaa aatctcattc cgtcgctttg cattgattga aaaaacagac gcacaacact
    15061 ttggagcata ccaacataac ggtggacgta tcggtgttat ttcagttgtt gaaggtggag
    15121 acgaagcact tgctaaacaa ttgtcaatgc acatcgcagc gatgaaacca acagttcttt
    15181 cttacaaaga attggatgag caattcgtta aagatgagtt ggcacaattg aatcacgtta
    15241 tcgaccaaga caacgaaagc cgtgcaatgg ttaataaacc agctcttcca cacttgaagt
    15301 atggatcaaa agctcaatta actgatgatg ttattgctca agctgaagct gacatcaaag
    15361 ctgaattggc tgcagaaggc aaaccagaaa aaatctggga caaaattatt ccaggtaaaa
    15421 tggatcgctt catgcttgat aacactaaag ttgaccaagc ttacacactt cttgcacaag
    15481 tttacatcat ggatgacagc aagacagttg aagcatacct tgaatcagtt aacgcttcgg
    15541 tagttgagtt tgctcgcttt gaagttggtg aaggtatcga gaaagctgca aacgacttcg
    15601 aagctgaagt tgcagctaca atggcagcag ccttgaataa ctaataatag aaaggaagca
    15661 atttgcttcc ttttttgtat agggcttata tttttcctga aattgttttt cccccagtct
    15721 cactcgatta gaataattaa aacaaaaagc cctataataa gggctaagtg catttaggag
    15781 actatacttc aaattcatag taagcgcgtc ataacaaggt atctatccta attccccaac
    15841 ttccagtata ctagataaaa aataattatc ggaggaaagg tattgtacta tgatgattca
    15901 actaagtgat ttaggtcaag ttcacattgt ttgtggcaag acagatatga ggcagggaat
    15961 agactcatta gcctatgtag ttaaaaccca ctttgaattg gattctttct ccggtcaagt
    16021 ctttctcttt tgtggtggac gtaaagaccg ctttaaagtc ctttactggg atggtcaagg
    16081 attttggcta ctatataaac gctttgagaa cggaaaactg acttggctaa gtacagaaaa
    16141 ggatgtcaaa gctctcacat ctgaacaagt agattggctt atgaagggct tttctatcac
    16201 tccaaaaata tagtagattg aaactagaat agtacacctc tgcttctaaa acattgttag
    16261 aaatcgattt gattgtcctg atcgatttgt cctgttctta tttcatttta ctataaatcc
    16321 atcagaaagt tgtgatttct attgaaatga ggactttctt tttacactca tctgctttca
    16381 aaaagcattc tagtccatct ccgattaacg atggacttta tcacctcctt ctccagtcct
    16441 tgtatgacat cttccaaagt tcgaaaggct ttattcttaa atccacgttt acgaatctct
    16501 ttccacactt gttcaatggg gttcatctct ggtgtgtatg gaggaataaa tgcaaagcca
    16561 atattagtcg gaatctttaa ggtacttgat ttatgccata tagcattgtc cataacgagt
    16621 aaaagataat catctggata agcttgtgaa agctcctatt cctaaagccc ctttagcgca
    16681 tagctttggc tcagcttcta ttatcgctca caccatccat cagaagttta atctgaaggt
    16741 acccaattat cgccaagaag aagattggac taagatgggt ttaccaatca cacgcaagga
    16801 aatctctaat tggcatatca agacgagtca atactatttg gagcccctct ataacctctt
    16861 gcgagagaga ctattgactc agcccttact tcatgcggat gaaacttctt atagggtgct
    16921 agagagtgat agtcagctga cttactattg gacttttttg tctgggaaag ctgagaaaca
    16981 agggattacg ctttaccatc atgtcctaat cgatttgttc atatcttatt tcaatccatt
    17041 ataaatagtg agaaatatct atcctatctt ctagaatgtc ttccaaacgt ggaaactctc
    17101 gtaaacaaag aggttttaga ggcttattta ccatggacta aagttgtaca agaaaagtgc
    17161 aaataagaaa tctccagatt aggaactatc cgtgagttct ctagtctgga gatttttcaa
    17221 tatacttcgt tattggacgg ttacaattca taaagtgctg tagagagata acgttcaccg
    17281 ttatctggtg ctaggacaag gactttttta cctgtaccta attttttggc aacctcgatg
    17341 gctccgtaga tagctgcagc tgaggaaatc cctacaagga agccttcttt tccaccaatt
    17401 tcacgtccga gtgcaagagc gtcatctgat gttacacgaa cgataccatc ataggcttta
    17461 gtatcaagtg tatcaggaat aaatccagct gagatacctt gaattttgtg aggaccaggt
    17521 ttttcaccag atttttcacc agatagaata gcagattcat ctgcttctac tgcaaaaact
    17581 tgaatgttag aattttctga tttgagtgca tgagaaacac cagaaatcgt tccaccggta
    17641 cctactccag caacaaaggc atctaatcca tctttaccga aagcagctag tatctcagct
    17701 cctgttgttc tttcgtgtac ttctggatta gctggattgt caaattgaag aggaaggaaa
    17761 ccatcacgtt cagcagcgat ttcttgagcc ttagcaatag cacctttcat tccctcgcta
    17821 ccaggagtta ggacgagttc agcaccataa gcttggataa ttttacgtcg ttctacactc
    17881 atagtttcag gcataacgat gacgacttta taccctttag cagcacctac ccatgaaagt
    17941 ccaataccag tgtttccact tgttgcttca acaatagtag aaccaggttt cagaatacca
    18001 tcttgttcag ctttttcaat catgctaagg gcaatacggt cttttacaga tgaaccagga
    18061 ttaaatgctt caagctttat atagacgtct gcagcacctt ctggcacgat gttgttaagt
    18121 ttaacaatcg gtgtttgacc gattaattca gtaatgttgt tataaataga catatgaata
    18181 cctctttgta taagatatct ttagtataac gctatccata ccatttgtaa aatatagaaa
    18241 tcctatcgaa gcgataggat ttctatatat catagatcaa agtcattaat tttcaagtga
    18301 ttgcgttcag ttaattctag taaataggtg tgaaggaaaa cgatatcagg tttcgtttga
    18361 attgcttcat tttctagtat attttggtac gcatcttttt attttgctcg ctaaagtagt
    18421 tgtgagaatg tcaacgaaac gctgtattat tcattgttta cactaagttt acaggaactt
    18481 caacctctcg taaaccttgg tcagttaaag tgacttttcc attaaaaaac tccacaagtg
    18541 cagctttaat agtttctttt tcttctttat caacataaat catcgtatcg acttgatctg
    18601 taaagtttgt atccagctcc atgagatcat gttctttaag gaagttactg tactcttggt
    18661 actgagcata agacatttga atagcaatgc cagcctgttc ttttatttca ataataccaa
    18721 tttctttgac agctaaggcg acactgccgg cgtaagcacg aattagtcct ccagcgccta
    18781 gtttaatacc accaaagtag cgtgtcacga ccacacagac attggtgaga ttgtgatttt
    18841 ctagtacccc aagcatggga acaccagcag taccactagg ctcaccatca tcacttgtac
    18901 gtttaatttc actacgttct ccaataataa aggcagagca gttatgtgtc gctttgtagt
    18961 gttctttttt gatggcagta atgaagtcac gagcctcttc ttcgctataa acacgcttgg
    19021 catggcagat aaagcgagat tttttgattt cttcttggac ttggccgtcc tctttaattg
    19081 tcctaaattc catgatttaa ttgtactaaa aaataatcta aagtgctagt ttttacgaat
    19141 aaagaagtat gaaagtaaat ttagattatc tcggtcgttt atttactgag aatgaattaa
    19201 cagaagaaga acgtcagttg gcggagaaac ttccagcaat gagaaaggag aaggggaaac
    19261 ttttctgtca acgttgtaat agtactattc tagaagaatg gtatttgccc atcggtgctt
    19321 actattgtcg agagtgcttg ctgatgaagc gagtcagaag tgatcaaact ttatactatt
    19381 ttccgcagga ggattttccg aagcaagatg ttctcaaatg gcgcggccaa ttaactcctt
    19441 ttcaagagaa ggtgtcagag ggactgcttc aagcagtaga caagcaaaag ccaaccttag
    19501 ttcatgcggt aacaggagct ggaaagacag aaatgattta tcaagtagtg gctaaagtga
    19561 tcaatgcggg tggtgcagtg tgtttggcta gtcctcgcat agatgtttgt ttggagctgt
    19621 acaagcgcct gcaacaggat ttttcttgcg ggatagcttt gctacatgga gaatcggaac
    19681 cttattttcg aacaccacta gttgttgcaa caacccatca gttattgaag ttttatcaag
    19741 cttttgattt gctgatagtg gatgaagtag atgcttttcc ttatgttgat aatcccacgc
    19801 tttaccacgc tgtcaagaat agtgtaaagg agaatggatt gagaatcttt ttaacagcga
    19861 cttcgaccaa tgagttagat aaaaaggtcc gtttaggaga actaaaaaga ctgagtttac
    19921 cgagacggtt tcatggaaat ccgttgatta ttccaaaacc aatttggtta tcggatttta
    19981 atcgctactt agacaagaat cgtttgtcac caaagttaaa gtcctatatt gagaagcaga
    20041 gaaagacagc ttatccgtta ctcatttttg cttcagaaat taagaaaggg gagcagttag
    20101 aagaaatctt acaggagcaa tttccaaatg agaaaattgg ctttgtatct tctgtaacag
    20161 aggatcgatt agagcaagta caagcttttc gagatggaga actgacaata cttatcagta
    20221 cgacaatctt ggagcgtgga gttaccttcc cttgtgtgga tgttttcgta gtagaggcca
    20281 atcatcgttt gtttaccaag tctagtttga ttcagattgg tggacgagtt ggacgaagca
    20341 tggatagacc gacaggagat ttgcttttct tccatgatgg gttaaatgct tcaatcaaga
    20401 aggcgattaa ggaaattcag atgatgaata aggaggctgg tctatgaagt gcttgttatg
    20461 tgggcagact atgaagactg ttttaacttt tagtagtctc ttacttctga ggaatgatga
    20521 ctcttgtctt tgttcagact gtgattctac ttttgaaaga attggggaag agaactgtcc
    20581 aaattgtatg aaaacagagt tgtcaacaaa gtgtcaagat tgtcaacttt ggtgtaaaga
    20641 aggagttgaa gtcagtcata gagcgatttt tacttacaat caagctatga aggatttttt
    20701 cagtcggtat aagtttgatg gagacttcct gttaagaaaa gttttcgctt catttttaag
    20761 tgaggagttg aaaaagtaca aagagtatca atttgttgta attcccctaa gtcctgatag
    20821 atatgctaac agaggattta atcaggttga gggcttggta gaggcagcag gctttgagta
    20881 tctggattta ttagagaaaa gagaagagag agccagttct tctaaaaatc gttcagagcg
    20941 cttggggaca gaacttcctt tctttattaa aagtggagtc actattccta aaaaaatcct
    21001 acttatagat gatatctata ctacaggagc aactataaat cgtgttaaga aactgttgga
    21061 agaagctggt gctaaggatg taaaaacatt ttcccttgta agatgaggaa aaaatttgca
    21121 aaaatagaat gaaagcgtta taataaaatc ataaaaacaa aaatgtttag aaagaaggta
    21181 ctcatatgat taaatatagt atccgtggtg aaaacctaga agtaacagaa gcaattcgtg
    21241 attatgtagt ttctaaactc gaaaagatcg aaaagtactt ccaaccagaa caagagttgg
    21301 atgcccgaat taacttaaaa gtttatcgtg aaaaaacggc taaagtggaa gtaacgattc
    21361 cgcttggatc tattactctc cgcgcagaag atgtatctca agatatgtat ggttcaattg
    21421 accttgtaac tgataaaatt gaacgtcaga ttcgtaaaaa taaaacaaaa atcgagcgta
    21481 aaaataaaaa taaggtagca actggtcaat tatttacaga tgctttggtg gaagattcaa
    21541 atgttgtcca gtctaaagtt gttcgttcaa aacaaattga tttaaaacca atggatttgg
    21601 aagaagcaat tctacaaatg gatttattgg ggcatgattt ctttatctat gtggatgttg
    21661 aagatcagac aaccaatgtg atttatcgtc gtgaggatgg cgaaattggt ttgttagagg
    21721 ttaaagaatc ttaatttcaa tatgtgtaaa ggtaggttta ctgaattgta aactaccttt
    21781 ttcttataat tgatagaatt actgattaca cttttaaaaa gtcgcttttt gaggggtatt
    21841 atggtataat gggtgccaag aggttttgaa tgaaaaaatt ttatgtaagt ccaatttttc
    21901 ctattctagt aggattgatt gcgtttggag tcttatccac tttcattatt tttgttaata
    21961 ataatctgtt gacggtttta attttgtttc tttttgtagg aggctatgtt tttttattta
    22021 agaaactgag agtgcattat acaaggagtg atgtagaaca gatacagtat gtaaaccacc
    22081 aagcggaaga aagtttgaca tctctattgg aacaaatgcc tgtaggtgtt atgaaattga
    22141 atttatcttc tggagaggtt gagtggttta atccctatgc tgaattgatt ttgaccaagg
    22201 aagatggtga ttttgattta gaagctgttc aaacgattat caaggcttca gtaggaaatc
    22261 cgtctactta tgccaagctt ggtgagaagc gttatgctgt tcatatggat gcttcttccg
    22321 gtgttttgta ttttgtagat gtatccaggg aacaagccat aacagatgaa ttggtaacaa
    22381 gtcgaccagt gattgggatt gtctctgtgg ataattatga tgatttggag gatgcaactt
    22441 ctgagtcaga tattagtcaa atcaatagtt ttgtagctaa ttttatatca gagttttcag
    22501 aaaaatacat gatgttttct cgtcgggtaa gtatggatcg attttatcta tttactgact
    22561 acacggtgct tgagggcttg atgaatgata aattttctgt tattgatgct ttcagagaag
    22621 agtcgaaaca gagacagttg cccttgacct taagtatggg attttcttat ggcgatggaa
    22681 atcatgatga gatagggaaa gttgctttgc tcaatttgaa cttggctgaa gtacgtggtg
    22741 gcgaccaggt ggttgttaag gaaaacgacg aaacgaaaaa tccagtttat tttggtggtg
    22801 ggtctgctgc ttcaatcaag cgtacacgga ctcgtacgcg cgctatgatg acagctattt
    22861 cagataagat tcggagtgta gatcatgttt ttgtagtcgg tcacaaaaat ttagacatgg
    22921 atgctttggg ctctgctgta ggtatgcagt tgttcgccag caatgtgatt gaaaatagct
    22981 atgctcttta tgatgaagaa caaatgtctc cagatattga acgagctgtt tcattcatag
    23041 aaaaagaagg agttacgaag ttgttgtctg ttaaggatgc aatggggatg gtgaccaatc
    23101 gttctttgtt gattcttgta gaccattcaa agacagcctt aacattatca aaagaatttt
    23161 atgatttatt tacccaaacc attgttattg accaccatag aagggatcag gattttccag
    23221 ataatgcggt tattacttat atcgaaagtg gtgcaagtag tgccagtgag ttagtaacgg
    23281 aattgattca gttccagaat tctaagaaaa atcgtttgag tcgtatgcaa gcaagtgtct
    23341 tgatggctgg tatgatgttg gatactaaaa atttcacctc gcgagtaact agtcggacat
    23401 ttgatgttgc tagctatctc agaacgcgcg gaagtgatag tattgctatc caggaaatcg
    23461 ctgcgacaga ttttgaagaa tatcgtgagg tcaatgaact tattttacag gggcgtaaat
    23521 taggttcaga tgtactaata gcagaggcta aggacacgaa atgctatgat acagttgtta
    23581 ttagtaaggc agcagatgcc atgttagcca tgtcaggtat tgaagcgagt tttgttcttg
    23641 cgaagaatac acaaggattt atctctatct cagctcgaag tcgtagtaaa ctgaatgtac
    23701 aacggattat ggaagagtta ggcggtggag gccactttaa tttggcagca gctcaaatta
    23761 aagatgtaac cttgtcagaa gcaggtgaaa aactgacaga aattgtatta aatgaaataa
    23821 aggaaaagga gaaagaagaa tgaaagtaat ctttttagca gatgttaaag gaaaaggtaa
    23881 aaaaggcgaa attaaggaag taccaacagg gtatgcgcaa aactttctta tcaaaaagaa
    23941 tctagccaaa gaagcgactg ctcaagctgt aggtgaactt cgtggtaaac aaaaatcgga
    24001 agaaaaagct cacgctgaga tgattgcaga agggaaagca attaaagcac aacttgaagc
    24061 agaagaaact gttgtagaat ttgttgaaaa agttggtcca gatggtcgta cctttggttc
    24121 tattaccaat aagaagattg cagaagaatt acaaaagcaa tttggaatta agattgataa
    24181 acgtcatatt caagtacaag ctccgattcg agcggttggt ttgattgatg tgccagtgaa
    24241 aatctatcaa gatatcacaa gtgtaatcaa tcttcgtgtg aaagaaggat aagtttacac
    24301 cttcttgaca agattgtaaa aggaagggaa gtctgatggc agaagtagaa gagttacgag
    24361 tacaacctca agatatctta gctgagcaat ccgttttagg ggctatcttt attgatgaga
    24421 gtaaacttgt ttttgtgcga gaatacattg agtctcggga cttttttaag tatgcccatc
    24481 gtttgatttt ccaagccatg gtcgatttat ccgatcgtgg tgatgccata gatgcaacaa
    24541 cggttcgtac tatccttgat aatcaaggtg atttacagaa tattggtggc ttgtcttact
    24601 tggttgagat tgtcaattct gtgccaactt ctgctaatgc ggagtattat gctaagattg
    24661 ttgcagaaaa agcaatgcta cgtcgtttaa ttgccaagtt gacagagtct gtcaaccaag
    24721 cttacgaagc gtcacaacca gctgatgaaa ttattgctca ggcagaaaaa gggctgattg
    24781 atgtcagtga aaatgcaaat cgaagcgggt ttaagaacat tcgagatgtg ttgaatctca
    24841 actttggaaa tctggaagct cgctcgcaac aaacgaccga tattacaggt attgcgacag
    24901 gttatcgtga tttggatcat atgacaacag gacttcatga ggaggagttg attatcttag
    24961 cagctcgtcc agcagttggt aagacagcat ttgccttgaa tatcgctcag aacattggga
    25021 ctaagttgga caaaacggtt gctatttttt cactcgaaat gggtgcggaa agcttggtag
    25081 atcgtatgtt agctgcagaa ggcttagtgg agtcacattc tatccgtaca gggcaattga
    25141 cagatgagga gtggcaaaaa tatactattg ctcagggtaa tctagctaac gccagtatct
    25201 atatcgatga tacgccaggt attcggatta cagagattcg ttctcgttct cgtaaattgg
    25261 ctcaagaaac tggaaatctt ggtttgattt tgatagacta tttgcaactt atcacgggaa
    25321 ctggtcgaga aaatcgtcaa caagaagttt ctgaaatttc tcgtcagttg aaaatactag
    25381 ccaaggaatt gaaggttcca gtaatcgctc tgagtcagct ttctcgtggt gtagaacaac
    25441 gtcaggacaa gagaccggtc ttgtctgata ttcgtgaatc tgggtctatt gagcaggacg
    25501 ctgatatcgt agcttttctc tatcgcgatg actactatga acgtggtggt gaagaagagg
    25561 agggtatccc aaataataag gtggaagtta ttatcgagaa aaaccgtagt ggagctcgtg
    25621 gaacagtgga attgattttc caaaaagaat acaataaatt ttcaagtatc tcaaagaggg
    25681 aggcataaga tgtcagatgc atttacagat gtagccaaga tgaaaaaaat caaagaagaa
    25741 atcaaggcac atgagggaca agtcgtagaa atgactttgg agaatggtcg taagcgccaa
    25801 aaaaatagat taggtaagct aattgaagtt tatccatccc tatttattgt ggagtttggg
    25861 gatgtggaag gagataaaca agttaatgtt tacgttgaat cctttactta ctcagatatt
    25921 cttacagaaa agaatttgat tcattatctt gactaaagtg agaaattttc tcactttttc
    25981 ttttttctcc gaataattta ggtgaaggca atcatcgctt tatattattt ttcaaggagg
    26041 aagaatgaaa attttaccgt ttatagcaag aggaacaagt tattacttga agatgtcagt
    26101 taaaaagctt gttccttttt tagtagtagg attgatgcta gcagctggtg atagtgtcta
    26161 tgcctattcc agaggaaatg gatcgattgc gcgtggggat gattatcctg cttattataa
    26221 aaatgggagc caggagattg atcagtggcg catgtattct cgtcagtgta cttcttttgt
    26281 agcctttcgt ttgagtaatg tcaatggttt tgaaattccg gcagcttatg gaaatgcgaa
    26341 tgaatgggga catcgttctc gtcgggaagg ttatcgtgta gataatacac cgacgattgg
    26401 ttccattact tggtctactg caggaactta tggtcatgtt gcctgggtgt caaatgtaat
    26461 gggagatcag attgagattg aggaatataa ctatggttat acagaatcct ataataaacg
    26521 aattataaaa gcaaacacga tgacaggatt tattcatttt aaagatttgg atggtggcag
    26581 tgttgggaat agtcaatcct caacttcaac aggcggaact cattatttta agaccaagtc
    26641 tgctatcaaa actgaacccc tagttagtgc aactgtgatt gattactatt atcctgggga
    26701 gaaggttcat tatgatcaga tacttgaaaa agacggctac aagtggttga gttatacggc
    26761 ttataacgga agtcgtcgct atatacagct agagggagtg acttcttcac aaaattatca
    26821 gaatcaatca ggaaatatct ctagctatgg atccaataat agttcaactg tcggttggaa
    26881 gaaaataaat ggtagttggt atcatttcaa atcaaatggt tctaaatcaa caggatggct
    26941 gaaagatggt tctagctggt attatttgaa attatctggt gaaatgcaga caggatggtt
    27001 aaaggagaat ggctcgtggt attatctggg tagttcaggg gcaatgaaaa caggctggta
    27061 ccaggtctct ggtaagtggt attattctta ctcttcaggc gccttagctg ttaatacgac
    27121 ggtggatggc tacagagtaa acagtgatgg agaacgagta tagaaaattg gagtaggaga
    27181 aatttcctgc tcttttctgt aagcagtttc cttgacattt ttcttatttt gcgctatcat
    27241 atatacatat aatatatggg agtctgtgta cagtctgaga ggaagtgtta aacttcgacc
    27301 gcacctgatc tgggtaatgc cagcggaggg aacgatactt agtctaattt tgcacctttt
    27361 ccatgtatgg taaaggtttt tcttttttta aaaaggaaaa cgagaagagg aggttcttat
    27421 gaaagcaagc attgccttgc aagttttacc cctagcacag gggattgatc ggatagctgt
    27481 tattgatcag gtcattgctt atctgcaaac tcaagaagtg acgatggtag tgacaccatt
    27541 tgaaacggtc ttggaagggg agtttgatga gcttatgcgc attctaaaag aagcgctgga
    27601 agtggcaggg cagggggcag acaatgtctt tgccaatgtc aaaataaatg taggagagat
    27661 tttaagtatt gatgagaaac ttgagaagta tactgagacg acacattagt ctattgggct
    27721 ttctcggagt cttgtcaatc tggcagttag caggttttct taaacttctc cccaagttta
    27781 tcctgccgac acctcttgaa attctccagc cctttgttcg tgacagagaa tttctctggc
    27841 accatagctg ggcgaccttg agagtggctt tactggggct gattttggga gttttgattg
    27901 cctgtcttat ggctgtactc atggatagtt tgacttggct caatgacctg atttacccta
    27961 tgatggtggt cattcagacc attccgacca ttgccatagc tcctatcctg gtcttgtggc
    28021 taggttatgg gattttgccc aagattgtct tgattatctt aacgacaacc tttcccatca
    28081 tcgtcagcat tttggacggt tttaggcatt gcgacaagga tatgctgacc ttgtttagtc
    28141 tgatgcgggc caagccttgg caaatcctgt ggcattttaa aattccagtc agcctgccct
    28201 acttttatgc tggtctgagg gtcagtgtct cctacgcttt tatcacaaca gtggtatctg
    28261 agtggttggg aggctttgag gggcttggtg tttatatgat tcagtccaag aaattgtttc
    28321 agtatgatac catgttcgct attattattc tggtatcgat tatcagcctt ttgggtatga
    28381 agttggtcga tattagtgaa aaatatgtta ttaaatggaa acgttcgtag aattagaatg
    28441 tttctgaaaa agaaaagagg aaatcaaaat gaagaaaaca tggaaagtgt ttttaacgct
    28501 tgtaacagct cttgtagctg ttgtgcttgt ggcctgtggt caaggaactg cttctaaaga
    28561 caacaaagag gcagaactta agaaggttga ctttatccta gactggacac caaataccaa
    28621 ccacacaggg ctttatgttg ccaaggaaaa aggttatttc aaagaagctg gagtggatgt
    28681 tgatttgaag ttgccaccag aagaaagttc ttctgacttg gttatcaacg gaaaggcacc
    28741 atttgcagtg tatttccaag actacatggc taagaaattg gaaaaaggag caggaatcac
    28801 tgccgttgca gctattgttg aacacaatac atcaggaatc atctctcgta aatctgataa
    28861 tgtaagcagt ccaaaagact tggttggtaa gaaatatggg acatggaatg acccaactga
    28921 acttgctatg ttgaaaacct tggtagaatc tcaaggtgga gactttgaga aggttgaaaa
    28981 agtaccaaat aacgactcaa actcaatcac accgattgcc aatggcgtct ttgatactgc
    29041 ttggatttac tacggttggg atggtatcct tgctaaatct caaggtgtag atgctaactt
    29101 catgtacttg aaagactatg tcaaggagtt tgactactat tcaccagtta tcatcgcaaa
    29161 caacgactat ctgaaagata acaaagaaga agctcgcaaa gtcatccaag ccatcaaaaa
    29221 aggctaccaa tatgccatgg aacatccaga agaagctgca gatattctca tcaagaatgc
    29281 acctgaactc aaggaaaaac gtgactttgt catcgaatct caaaaatact tgtcaaaaga
    29341 atacgcaagc gacaaggaaa aatggggtca atttgacgca gctcgctgga atgctttcta
    29401 caaatgggat aaagaaaatg gtatccttaa agaagacttg acagacaaag gcttcaccaa
    29461 cgaatttgtg aaataatgac agaaattaga ctagagcacg tcagttatgc ctatggtcag
    29521 gagaggattt tagaggatat caacctacag gtgacttcag gcgaagtggt ttccatccta
    29581 ggcccaagtg gtgttggaaa gaccaccctc tttaatctaa tcgctgggat tttagaagtt
    29641 cagtcaggga gaattgtcct tgatggtgaa gaaaatccca aggggcgcgt gagttatatg
    29701 ttgcaaaagg atctgctctt ggagcacaag acggtgcttg gaaatatcat tctgcccctc
    29761 ttgattcaaa aggtggataa ggcagaagct atttcccgag cggataaaat tcttgcgacc
    29821 ttccagctga cagctgtaag agacaagtat cctcatgaac ttagcggtgg gatgcgccag
    29881 cgtgtagcct tactccggac ctaccttttt gggcacaagc tctttctctt agatgaggcc
    29941 tttagcgcct tggatgagat gacaaagatg gaactccacg cttggtatct tgagattcac
    30001 aagcagttgc agctaacaac cctgatcatc acgcatagta ttgaggaggc cctcaatctc
    30061 agcgaccgca tctatatctt gaaaaatcgc cctgggcaga ttgtttcaga aattaaacta
    30121 gattggtctg aagatgagga caaggaagtc caaaagattg cctacaaacg tcaaatcttg
    30181 gcagaattag gcttagataa ttagaaaaat agggagttgg tgaagattat cctttaccag
    30241 cgcccttttt cttttaaaaa tgagaaaatt tcggtataat agtcaaacaa ggtcaaggtt
    30301 taaagagaga ggtgggtttg ttatgagatt taaaaataca tcggatcata ttgaggccta
    30361 catcaaggcg attttagatc aatctggtat cgtggagttg caacggagtc agttggcaga
    30421 tacctttcag gttgttccta gtcagattaa ctacgtgatc aagacacgct ttacggaaag
    30481 tagaggctac ttggttgaaa gtaagcgtgg tggcggaggc tacattcgta taggacggat
    30541 tgagttttct agtcatcatg aaatgctccg ggagctgctt tactcgattg gtgagcgagt
    30601 cagtcaagaa atttatgagg atattctcca gcttttggtt gagcaggaat tgatgaccaa
    30661 gcaggagatg aatttgctag aatcagtagc tttggatcgc gttttaggag aagaagctcc
    30721 agttgttcga gcaaacatgc tacgtcagat catacaagag gtagatagaa aagggaagta
    30781 agatgaacta ttcaaaagca ttgaatgaat gtatcgaaag tgcctacatg gttgctggac
    30841 attttggagc tcgttatcta gagtcgtggc acttgttgat tgccatgtct aatcacagtt
    30901 atagtgtagc aggggcaact ttaaatgatt atccgtatga gatggaccgt ttagaagagg
    30961 tggctttgga actgactgaa acggactata gccaggatga aacttttacg gaattgccgt
    31021 tctcccgtcg tttgcagatt ctttttgacg aagcagagta tgtagcgtca gtggtccatg
    31081 ctaaggtgct agggacagag cacgtcctct atgcgatttt gcatgatggc aatgccttgg
    31141 cgactcgtat cttggagagg gctggttttt cttatgaaga caagaaagat caggtcaaga
    31201 ttgctgctct tcgtcgaaat ttagaagaac gggcaggctg gactcgtgaa gatctcaagg
    31261 ctttacgcca acgccatcgt acagtagctg acaagcaaaa ttctatggcc aatatgatgg
    31321 gcatgccgca gactcctagt ggtggtctcg aggactatac gcatgatttg acagagcaag
    31381 cgcgttctgg caagttagaa ccagtcatcg gtcgggacaa ggaaatctca cgtatgattc
    31441 aaatcttgag ccggaagact aagaacaacc ctgtcttggt tggggatgct ggtgtcggga
    31501 aaacagctct ggcgcttggt cttgctcagc gtattgctag tggtgacgtg cctgcggaaa
    31561 tggctaagat gcgcgtgtta gaacttgatt tgatgaatgt cgttgcaggg acacgcttcc
    31621 gtggtgactt tgaagaacgc atgaataata tcatcaagga tattgaagaa gatggccaag
    31681 tcatcctctt tatcgatgaa ctccacacca tcatgggttc tggtagcggg attgattcga
    31741 ctctggatgc ggccaatatc ttgaaaccag ccttggcgcg tggaactttg agaacggttg
    31801 gtgccaccac tcaggaagaa tatcaaaaac atatcgaaaa agatgcggca ctttctcgtc
    31861 gtttcgctaa agtgacgatt gaagaaccaa gtgtggcaga tagtatgact attttacaag
    31921 gtttgaaggc gacttatgag aaacatcacc gtgtacaaat cacagatgaa gcggttgaaa
    31981 cagcggttaa gatggctcat cgttatttaa ccagtcgtca cttgccagac tctgctatcg
    32041 acctcttgga cgaagcagca gcaacagtgc aaaataaggc aaagcatgta aaagcagacg
    32101 attcagattt gagtccagct gacaaggccc tgatggatgg caagtggaaa caggcagccc
    32161 agctaatcgc aaaagaagag gaagtacctg tctacaaaga cttggtgaca gagtctgata
    32221 ttttgaccac cttgagtcgc ttgtcaggaa tcccagttca aaaactgact caaacggatg
    32281 ctaagaagta tctaaatctt gaagcagaac tccataaacg tgtcatcggt caagatcaag
    32341 ctgtttcaag cattagtcgt gcgattcgcc gtaatcagtc aggaattcgc agtcacaagc
    32401 gtccgatcgg ttcctttatg ttcctaggac ccacaggtgt cggtaagacc gagttggcca
    32461 aagctttggc agaagttctc tttgatgaag aatcagccct tatccgcttt gatatgagtg
    32521 agtatatgga gaaattcgca gccagccgtc taaatggagc tcctccaggc tatgtgggtt
    32581 acgaagaagg tggggagttg acagagaagg ttcgcaataa accctattcc gttctcctct
    32641 ttgatgaggt agagaaggcc cacccagata tctttaatgt tctcttgcag gttctggatg
    32701 acggtgtctt gacagatagc aagggacgca aggtcgattt ttcaaatacc attatcatta
    32761 tgacatcgaa tctaggtgcg actgcccttc gtgatgataa gactgttggt tttggggcta
    32821 aggatattcg ttttgaccag gaaaatatgg aaaaacgcat gtttgaagaa ctgaaaaaag
    32881 cttatagacc ggaattcatc aaccgcattg atgagaaggt ggtcttccat agcctatcta
    32941 gtgaccatat gcaggaagtg gtgaagatta tggtcaagcc tttagtggca agtttggctg
    33001 aaaaaggcat tgacttaaaa ttacaagctt cagctctgaa attgttagca aatcaaggat
    33061 atgacccaga gatgggagct cgcccacttc gcagaaccct gcaaacagaa gtggaggaca
    33121 agttggcaga acttcttctt aagggagatt tagtggcagg caacacactt aagattggtg
    33181 tcaaagcagg ccagttaaaa tttgatattg cataaaagaa taaaagtatc agcatctgac
    33241 cataagtcac agtggagtga aactcaatga aaatcaaaga gcaaactagg cagctagctg
    33301 caggttgctc aaaacactgt tttgaggttg cagatagagc tgacatggtt tgaagagatt
    33361 ttcgaagagt atgaaactaa aacctatagc ttctaaacga tccgtggttt tcaccattca
    33421 acacaaaatt catatgttta ttaccctccg tcgtatttgt cttagagcgt gtgtagtaga
    33481 aaaagagcag tcttatctga aatttttatt ctttcaaaag agacctgttt cttttttgca
    33541 tgtcaaatcc gttctagctg gtatttgaaa aatcaaacta atattcaatg aaaatcaaag
    33601 aacaaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt gtagatagag
    33661 ctgacgtggt ttgaagagat tttcgaagag attttcgaag agtataagct gcaagatgaa
    33721 tgattttctt gtattgacgt tgttgttgac aaaaagtagc ggataaatga aatccattcc
    33781 attatcatag atgataggct ggtaggaaat tttcaaatag catataggaa atagatgtat
    33841 ggagttctgg tagtagaaag ggagagagat gaacatttta gttgcagatg acgaggaaat
    33901 gattagagaa ggaattgcag catttctgac agaagagggt tatcatgtca ttatggctaa
    33961 ggatggacaa gaggtcttgg aaaaatttca agatctccct atccatctca tggtactgga
    34021 tttaatgatg cctaggaaga gtggttttga agtgttaaaa gaaatcaatc aaaagcacga
    34081 tattcctgtc atcgtcttga gtgctctggg agatgaaact actcagtcac aggtatttga
    34141 tctctatgct gatgatcatg tgacaaaacc tttttctttg gtactgcttg tcaagcgtat
    34201 taaggcgctt atcagacgtt actacgtcat agaggatctt tggcgatatc aggatgtaac
    34261 agtggatttt acctcttaca aagcacatta taaaaatgaa gaaattgatc tcaaaccaaa
    34321 ggaattactg gtactaaagt gtttgattca gcataaaaat caagttttaa gtagagagca
    34381 gatattggaa gaaatttcaa aagatgtagc tgatttacct tgtgataggg tcgttgatgt
    34441 ctatattcgt actcttcgca aaaaattagc tttagattgt atcgtgactg tgaaaaatgt
    34501 tgggtataag attagcttat gataaaaaat cctaaattat taaccaagtc ttttttaaga
    34561 agttttgcaa ttctaggtgg tgttggtcta gtcattcata tagctattta tttgaccttt
    34621 cctttttatt atattcaact ggagggggaa aagtttaatg agagcgcaag agtgtttacg
    34681 gagtatttaa agactaagac atctgatgaa attccaagct tactccagtc ttattcaaag
    34741 tccttgacca tatctgctca ccttaaaaga gatattgtag ataagcggct ccctcttgtg
    34801 catgacttgg atattaaaga tggaaagcta tcaaattata tcgtgatgtt agatatgtct
    34861 gttagtacag cagatggtaa acaggtaacc gtgcaatttg ttcacggggt ggatgtctac
    34921 aaagaagcaa agaatatttt gcttttgtat ctcccatata catttttggt tacaattgct
    34981 ttttcctttg ttttttctta tttttatact aaacgcttgc tcaatcctct tttttacatt
    35041 tcagaagtga ctagtaaaat gcaagatttg gatgacaata ttcgttttga tgaaagtagg
    35101 aaagatgaag ttggtgaagt tggaaaacag attaatggta tgtatgagca cttgttgaag
    35161 gttattcatg agttggaaag tcgtaatgag caaattgtaa aattgcaaaa tcaaaaggtt
    35221 tcctttgtcc gtggagcatc acatgagttg aaaacccctt tagccagtct tagaattatc
    35281 ctagagaata tgcagcataa tattggagat tacaaagatc atccaaaata tattgcaaag
    35341 agtataaata agattgacca gatgagccac ttattggaag aagtactgga gtcttctaaa
    35401 ttccaagagt ggacagagtg tcgtgagact ttgactgtta agccagtttt agtagatatt
    35461 ttatcacgtt atcaagaatt agctcattca ataggtgtta caattgaaaa tcaattgaca
    35521 gatgctacca gagtcgtcat gagtcttagg gcattggata aggttttgac aaacctgatt
    35581 agtaatgcaa ttaaatattc agataaaaat gggcgcgtaa tcatatccga gcaagatggc
    35641 tatctctcta tcaaaaatac atgtacgcct ctaagtgatc aagaactaga gcatttattt
    35701 gatatattct atcattctca aatcgtgaca gataaggatg aaagttccgg tttgggtctt
    35761 tacattgtga ataatatttt agaaagctat caaatggatt atagttttct cccttatgaa
    35821 cacggtatgg aatttaagat tagcttgtag acagattagt tttttattaa agttcatata
    35881 gggttaacat aagtgtgtta ttctttgtat agataaaaga aaggatacta atatggtatt
    35941 agcgattatt ttagtaacat tctttattcg attgattttt ttaaagcgtt cgatagagaa
    36001 tgagaaacga atccttagca atggcgggaa agaatttgga gttgagaata caaaacgatt
    36061 aactatggct catattgttt tttatctctc ttgcttggtt gaggcaatgg tgcacaagac
    36121 aatgtttgat ggtatgagca tggttggttt agtcttgctt attttttcta tgctgatgtt
    36181 gatgttggtg attcacttgt tgggagatat ttggacagtg aagcttatgc ttgtcaataa
    36241 tcacaaatat gtagatcata tcttgtttag gacagtaaaa catcctaatt actttttaaa
    36301 tattcttcct gagttgattg gcttgacctt gttgagtcat gcttatgtga cttttgtttt
    36361 agtttttcca gtttatgcag ttattttgta tcgacgaata gctgaagagg aaaagctatt
    36421 acatgaagtt ataatcccaa atggaagcat aaagagataa atacaaaatt cgatttatat
    36481 acagttcata ttgaagtgat atagtaaggt taaagaaaaa atatagaagg aaataattat
    36541 gtttgcatca aaaagcgaaa gaaaagtaca ttattcaatt cgtaaattta gtattggagt
    36601 agctagtgta gctgttgcca gtcttgttat gggaagtgtg gttcatgcga cagagaacga
    36661 gggaagtacc caagcagcca ctttttctaa tatggcaaat aaaagtcaga cagaacaagg
    36721 agaaatcaat atagaacgag ataaggcaaa gacagcggtc agtgaatata aagaaaaaaa
    36781 agtgagtgag atctatacaa aattagagag agatagacat aaagatactg tagatctagt
    36841 taacaagttg caagagatta agaacgagta tttgaataaa atagttgaat caacttcaaa
    36901 aatcgaaata cagggactta ttacaacaag tcgatcagaa ctagatgaag ctgtgtctaa
    36961 atataaaaag gccccatctt cttcgtcaag ttcaggctcc tccactaaac cagaaactcc
    37021 g
//
LOCUS       DAVKAO010000012        35899 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKAO010000012 DAVKAO010000000
VERSION     DAVKAO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.012"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(136..903)
                     /locus_tag="VWX16_000646"
     CDS             complement(136..903)
                     /locus_tag="VWX16_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9158037.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(900..1766)
                     /locus_tag="VWX16_000647"
     CDS             complement(900..1766)
                     /locus_tag="VWX16_000647"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9158038.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(1803..3170)
                     /gene="accC"
                     /locus_tag="VWX16_000648"
     CDS             complement(1803..3170)
                     /gene="accC"
                     /locus_tag="VWX16_000648"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9158039.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(3182..3604)
                     /gene="fabZ"
                     /locus_tag="VWX16_000649"
     CDS             complement(3182..3604)
                     /gene="fabZ"
                     /locus_tag="VWX16_000649"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9158040.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(3601..4086)
                     /locus_tag="VWX16_000650"
     CDS             complement(3601..4086)
                     /locus_tag="VWX16_000650"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9158041.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(4089..5324)
                     /gene="fabF"
                     /locus_tag="VWX16_000651"
     CDS             complement(4089..5324)
                     /gene="fabF"
                     /locus_tag="VWX16_000651"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9158042.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(5346..6077)
                     /gene="fabG"
                     /locus_tag="VWX16_000652"
     CDS             complement(5346..6077)
                     /gene="fabG"
                     /locus_tag="VWX16_000652"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9158043.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFAVAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(6111..7031)
                     /gene="fabD"
                     /locus_tag="VWX16_000653"
     CDS             complement(6111..7031)
                     /gene="fabD"
                     /locus_tag="VWX16_000653"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9158044.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(7024..7998)
                     /gene="fabK"
                     /locus_tag="VWX16_000654"
     CDS             complement(7024..7998)
                     /gene="fabK"
                     /locus_tag="VWX16_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9158045.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(8117..8341)
                     /locus_tag="VWX16_000655"
     CDS             complement(8117..8341)
                     /locus_tag="VWX16_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9158046.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(8401..9375)
                     /locus_tag="VWX16_000656"
     CDS             complement(8401..9375)
                     /locus_tag="VWX16_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9158047.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(9375..9809)
                     /locus_tag="VWX16_000657"
     CDS             complement(9375..9809)
                     /locus_tag="VWX16_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9158048.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(9903..10688)
                     /locus_tag="VWX16_000658"
     CDS             complement(9903..10688)
                     /locus_tag="VWX16_000658"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9158049.1"
                     /translation="MEHIIYQFEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            11013..12377
                     /locus_tag="VWX16_000659"
     CDS             11013..12377
                     /locus_tag="VWX16_000659"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9158050.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRELTPIKEEEILRQLVQKTKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGK
                     SKED"
     gene            12380..12751
                     /locus_tag="VWX16_000660"
     CDS             12380..12751
                     /locus_tag="VWX16_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9158051.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            12965..14239
                     /gene="serS"
                     /locus_tag="VWX16_000661"
     CDS             12965..14239
                     /gene="serS"
                     /locus_tag="VWX16_000661"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9158052.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(14264..15163)
                     /locus_tag="VWX16_000662"
     CDS             complement(14264..15163)
                     /locus_tag="VWX16_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9158053.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTNLPNWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(15273..15821)
                     /locus_tag="VWX16_000663"
     CDS             complement(15273..15821)
                     /locus_tag="VWX16_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9158054.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(16039..17361)
                     /locus_tag="VWX16_000664"
     CDS             complement(16039..17361)
                     /locus_tag="VWX16_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9158055.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFTALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSQVVIAETKKYFDK"
     regulatory      complement(17452..17540)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(17704..20040)
                     /locus_tag="VWX16_000665"
     CDS             complement(17704..20040)
                     /locus_tag="VWX16_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9158056.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLTSREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRVLKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(20144..20692)
                     /locus_tag="VWX16_000666"
     CDS             complement(20144..20692)
                     /locus_tag="VWX16_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9158057.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(20689..20991)
                     /gene="zapA"
                     /locus_tag="VWX16_000667"
     CDS             complement(20689..20991)
                     /gene="zapA"
                     /locus_tag="VWX16_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9158058.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            21078..21959
                     /locus_tag="VWX16_000668"
     CDS             21078..21959
                     /locus_tag="VWX16_000668"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9158059.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            21963..22577
                     /gene="lepB"
                     /locus_tag="VWX16_000669"
     CDS             21963..22577
                     /gene="lepB"
                     /locus_tag="VWX16_000669"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9158060.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            22704..25070
                     /locus_tag="VWX16_000670"
     CDS             22704..25070
                     /locus_tag="VWX16_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9158061.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMILAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQTCMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKNNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(25118..26401)
                     /locus_tag="VWX16_000671"
     CDS             complement(25118..26401)
                     /locus_tag="VWX16_000671"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9158062.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLTELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQSLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(26562..26954)
                     /locus_tag="VWX16_000672"
     CDS             complement(26562..26954)
                     /locus_tag="VWX16_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9158063.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNSFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNITEKLDKLVLEND"
     gene            complement(27253..27882)
                     /locus_tag="VWX16_000673"
     CDS             complement(27253..27882)
                     /locus_tag="VWX16_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9158064.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVCYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGASQINSAVKLNERNLSFIYSVLKGYSLEGW"
     gene            complement(27927..28166)
                     /locus_tag="VWX16_000674"
     CDS             complement(27927..28166)
                     /locus_tag="VWX16_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158065.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(28316..28582)
                     /locus_tag="VWX16_000675"
                     /pseudo
     CDS             complement(28316..28582)
                     /locus_tag="VWX16_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(28607..28918)
                     /locus_tag="VWX16_000676"
     CDS             complement(28607..28918)
                     /locus_tag="VWX16_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9158066.1"
                     /translation="MDWDFADTCWEKDPREYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHSELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(28843..29085)
                     /locus_tag="VWX16_000677"
     CDS             complement(28843..29085)
                     /locus_tag="VWX16_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9158067.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRILENTNMWLLTI"
     gene            complement(29086..29718)
                     /locus_tag="VWX16_000678"
     CDS             complement(29086..29718)
                     /locus_tag="VWX16_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9158068.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQATVYAFQHHLVGQEEF"
     gene            complement(29732..30727)
                     /locus_tag="VWX16_000679"
     CDS             complement(29732..30727)
                     /locus_tag="VWX16_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9158069.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(30724..31422)
                     /locus_tag="VWX16_000680"
     CDS             complement(30724..31422)
                     /locus_tag="VWX16_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9158070.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(31500..32510)
                     /locus_tag="VWX16_000681"
     CDS             complement(31500..32510)
                     /locus_tag="VWX16_000681"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9158071.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYIKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(32494..33501)
                     /locus_tag="VWX16_000682"
     CDS             complement(32494..33501)
                     /locus_tag="VWX16_000682"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9158072.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(33488..34441)
                     /gene="mvaD"
                     /locus_tag="VWX16_000683"
     CDS             complement(33488..34441)
                     /gene="mvaD"
                     /locus_tag="VWX16_000683"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9158073.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(34423..35301)
                     /gene="mvk"
                     /locus_tag="VWX16_000684"
     CDS             complement(34423..35301)
                     /gene="mvk"
                     /locus_tag="VWX16_000684"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9158074.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVS
                     AESPWRLYEEDTLSMAVYASLEYLDITEACVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETALSYGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(35423..35752)
                     /locus_tag="VWX16_000685"
     CDS             complement(35423..35752)
                     /locus_tag="VWX16_000685"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9158075.1"
                     /translation="MTRKVYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYFVN
                     YNGEWVQ"
BASE COUNT        10414 a         8322 c         6802 g        10361 t
ORIGIN      
        1 atttttgtaa gcttacatct acagtataca tcttttttgt ctattttata gcccatctcc
       61 tcaactgtca atttttcaac ctgaattgca tttttccata aaaaatgaga cctttctagt
      121 ctcatttagt cattcttagt attttctaaa tcgttgatag cgttcttcca gcaactcttc
      181 tagcggtttt tgtgaaagtc tagccagctc cgtttggagt tcttttttga cactcttaat
      241 cagttcttta ctagaaagtc ctacttcaga aatcacctta tccaccacgt ccatttctaa
      301 cagttcatgc gaagtgattt tcatcagttc tgctgcttcc atggcgcgag taccgtcctt
      361 ccataaaatg gaagcaaagc cttctggact gagaatggca tagatagaat tttccagcat
      421 ccagacacgg tccgcgacag ctagagccag agccccgcct gaaccacctt caccgataat
      481 aatggcgata ataggaactt tcaggtcact catttccatg agattgcgag cgatagcttc
      541 cccttgacca cgttcttccg ctccgacacc aggataagca cctgctgtat tgataaaggt
      601 cacaactgga cggccaaatt tctcagcctg tttcatcaac cgcagtgcct ttcggtagcc
      661 ttctggatgt ggttggccaa aattccgttt gaggttgtct tgcaaactct tgcctttttg
      721 gataccaacc actgttacag cttggtctcc aagccaacca ataccaccaa caactgcacc
      781 atcatcacga aaagaacggt caccatgtaa ttggataaat tcatcaaaaa tgcctgtcgc
      841 aaagtccaag gttgtcaagc gactctgctc acgcgcttct ctgactattt ttgcaatatt
      901 catctaggac tccctccatg caatctgact aggctagcaa tcgtatctgg taagtctctt
      961 cttttgacaa tagcatccac aaagccatgt tctaatagga attctgcctt ttggaaatcc
     1021 tcaggcaagc tttcacgaac cgtattttca atcacacgac gtccagcaaa accaaccaag
     1081 ctctgtggtt cagccagaat gatatcgcct tccatagcga aagaagctgt cacaccacca
     1141 gtcgttggat ctgtcaaaat ggtcaggtaa aagagaccag catttgaatg gcgtttaacc
     1201 gccgcagaga tcttagccat ctgcatgaga ctcatgattc cttcctgcat acgggctcca
     1261 ccagaggctg tgaataggac aactggcaat ttttcgacag tcgcatactc aaacaaacga
     1321 gtgatttttt cacctacaac cgtacccata gaagccatga taaagttaga atccataatc
     1381 ccaagagcca cagtctgacc tttaataaga gcagttcctg tcacaacggc ttcatgcaga
     1441 cctgtttttt cacgcataga tgccagtttc ttttggtaac cagggaaatg caagggatcc
     1501 ttgctttcaa tccctgtaaa caattctttg aaggttccca tatcaatcgt caaagccaag
     1561 cgttcttggg cagaaatacg aaaggtatag ctacagtgcg gacagatacg ttcacttccc
     1621 agatccttct gatagatggt atgcttacag cctggacact gggaaaataa ttcatctgga
     1681 acctctggct tagcttgagg tttttcccta accgaacgat tgggattgat tcgaatatac
     1741 ttatcttttt tactaaatag agccattgat tccccttttc ggtttaaact cttaaagtca
     1801 ttttattctt tttcttgata tttaggtaag aaggtttcca tcaagaagga agtatcataa
     1861 tccccagcaa tgacattgcg atctgaaatg aggtcaagct ggaaatctgc attggtctgc
     1921 actccttcaa tttctaattc atagagggca cgttgcattt tcatcaaggc gtcaaaacga
     1981 ttttcgccgt gtactatgat tttggcaatc atactatcat aataaggcgg aatggtataa
     2041 cctggataaa ctgctgaatc cacgcgcaag ccaactccac cacttggcag atagagatta
     2101 gtaatcttac ctggacttgg agcaaagtta aaggctgggt tttctgcatt gatacgacac
     2161 tcgatggcat gaccgcgtag gacaatatct tcttgcttaa cagacaaagg ctgacctgcc
     2221 gcaatgcaaa tctgttcctt aacgatatca acacctgaaa caaactctgt tactggatgt
     2281 tctacctgaa cacgagtatt catctccatg aaatagaaat tgctacttgc ttcatcaaga
     2341 agaaattcaa tggttcctgc attctcatag ccaacaaact ctgccgctcg aacagcagca
     2401 gcacctattt catgacgcag cgtttttccg attgcaatcg agggactttc ttccaaaacc
     2461 ttttggttat tcctttgaag agaacaatcc cgttcaccca agtgaatcac atgtccatgc
     2521 tcatcaccta ggatttgaac ctcaatgtgc cgagctggat agataacccg ttctatgtac
     2581 atggcaccat tgccataatt ggccttggcc tcactagagg cagtttcaaa ggcagaaacg
     2641 aggtcatctg gtttttcaac cttacgaatc cctttaccac ctccacctgc tgaagccttg
     2701 agcataacag gatagccaat tttttcagca acaatcaaag cttcttcaga gttatgcact
     2761 tctccatctg aacctggtat aacaggcaca cctgctttaa tcatctgagc acgcgcattg
     2821 atcttatccc ccatcatatc cataacatga ccagatggac cgataaactt gatacctact
     2881 tcttcacaca tggtcgcaaa tttggaattt tcactgagaa atccaaaacc agggtgaata
     2941 gcttctgcct cagtcaagac tgcagctgat agaactgcat taatattgag ataagactct
     3001 gttgccttgc caggaccaat acaaactgct tcatctgcca acagcgtatg aagagcttcc
     3061 ttatcagcag ttgaataaac cgctaccgtc gcaatcccca attcacgtgc cgcacggata
     3121 atacgaaccg caatttcacc acgattggca attaaaattt ttcgaaacat ggagaacctc
     3181 cttagttccc aattgcaaaa gtaagggtac cactggctgc aagcttgcca tccacttcag
     3241 cctttgcttc aaccacagct atggtgccac gacgttttac aaaagtcgct gtcataacca
     3301 attggtcgcc tggtacaact tgcttcttga acttaacctt atccatacca gcgtaaaaga
     3361 ccagttttcc tttattttca ggttttgata actccaacac accagcagtt tgcgccaagg
     3421 cttccataat cagaacacct ggcataactg ggtattgagg aaagtggccg ttaaagaaag
     3481 gctcgttgat ggtcacattt ttgatagcaa caatggtatc ctcgctcacc tccaagacac
     3541 ggtccactag aagcatagga taacggtggg gaagggcttc tttgattcct tgaatatcga
     3601 tcatttgata cgtaccaatc ctttaccaaa ctcaaccatt tcttcgttag agacgagaat
     3661 ttccgttacc acaccatcct taggagctgg gatttcattc atgactttca tggcttcgat
     3721 aattaccaat gtttgacctt ttttgacact atcaccaact gtaacgaagg caggtttatc
     3781 tggtccagca gccaagtaaa ccactccaac aagtggactc tctacaagat ttccctcact
     3841 agccacactt gcttcagctg gagctggaac ttcttctgct acagtctctg ctggagcaga
     3901 tgtaggagct actggactcg gtgttgctag aacgggtgct ggagcgactt gagttgcaac
     3961 ttcaggcaca ggtctcgctt cattcttgct aaactgcaac tcatccgtcc catttttata
     4021 agaaaattct ctcaaacttg actggtcaaa ttgagtcatc aagtctttaa tatcgtttaa
     4081 attcatactt atctattctc ccaacgtttg aaagcaagaa ctgcattgtg gcctccaaaa
     4141 ccaaaagtat ttgaaatagc gtatggaatt tctttctcca agccttgtcc ataaacgaca
     4201 ttagcttcga tataatctga tacttcactt gtcccagctg tcattggtac aaagttatga
     4261 cgcatagctt cgatggtgac gatagcttct actgcacccg cagcccccag caaatgtcct
     4321 gtaaaagact tggttgatga tacaggtact tccttaccaa gaacagctac gatagcacca
     4381 ctttctcctt tttcattggc aggagttgac gttccgtgag cattgacata ggctacttgc
     4441 tctggagaaa tctcagcttc ttccaaggct agtttgatgg ccttgatagc tccctgacct
     4501 tctggatgtg gagaagtcat gtggtaggca tcacaagtat ttccgtaacc aaccacttca
     4561 gccaggatag tagctccacg tttttcagcg tgttcaagac tttctagaac caacatccct
     4621 gaaccttcac ccataacaaa cccattgcga tccttatcaa atgggatcga agcacgagtt
     4681 ggatcctctg tagtagagag agctgttaag gcttggaaac cagcgatggc aaaaggtgtg
     4741 atagaagctt ctgttcctcc caccaacatc acatcttgga aaccaaactt aatggagcgg
     4801 aaggcatccc caatcgcatc atttgatgaa gagcaggcag tattgataga tttacaaaca
     4861 ccgtttgcac caaaacgcat ggctacattc ccagaagcca tatttggtaa agcttttgga
     4921 agagtcattg gtttgacacg tttgggtcct ttttcatgaa ggcgaagtac ctgatcttca
     4981 atttccttga ttccaccaat accagatgca acgataacac caaaacgatc cctattaaga
     5041 gcctctacat caagattggc atgatttaca gcctcttggg ctgcatacaa ggcatataaa
     5101 gaatagttat caaaacggtt ggtatctttt tttacaaagt atttatcgaa cggaaaatct
     5161 tggatttctg ccgcattatg cacatcaaag tcactatgat caaattttgt aatgccacca
     5221 atgccgattt tcccagttgc taaactattc caaaattctt ctggtgtatt tccgattgga
     5281 gatgttactc cataacctgt taccactact cgatttagtt tcattctttt cacctctagc
     5341 tttcgctaca tacttaagcc accatcaatg gcaaccactt gaccagttag ataatcttgg
     5401 cctgctaaaa atactgtcaa atctgcaacc tgctctgcct gcccaaattc tttcatcgga
     5461 atctgagcta gtgtagcttc cttaatctta tctgataaga tagctgtcat atcagactca
     5521 atcattcctg gagcaatcac attgactcgt atattccgac tagcgacctc gcgtgccaca
     5581 gacttggtaa agccaatcaa gccagcctta gaagcagcat agttagcttg accaatattc
     5641 cccatcaaac caacaacact agacatatta atgatagcac cttctctggc tttcatcatc
     5701 ggtttcaaga ctgattgtgt catattaaag gcaccagtca gattgacctt gagcactttt
     5761 tcaaaatctg cttctgtcat cttgagcata agagtatctt gggtaatccc tgcattgttg
     5821 accaaaacat ctactgaacc cagttctgca atagcttgat caatcatacg cttagcgact
     5881 gcaaaatctg atacatctcc tgaaatggga accaccttga taccatagtt tgaaaactca
     5941 gcgagcaatt cttctgagat tgccccacga ctgtttaaga caatgttggc tcctgcttga
     6001 gcaaacttgt gggcgatggc aagaccaatt ccacgactcg aacctgtaat aaagatattt
     6061 ttatgttcta gtttcatttt tttcctttca aaacttctac ttattttagt ctatttttct
     6121 aaaagtgcta ctaaactcgc ttgatcttcc acatgagcta agtgagcagt ttgatcaatt
     6181 tttttaacaa aacctgacaa gactttcccc ggtccaatct cgataaagtt gcttatgcct
     6241 gcttcttgca tgaccccaat actttcatag aaacgaacgg gttccttgac ctgacgcgtc
     6301 aagagctgag caatgtcctc tttttgcatc acagcagctt ctgtattgcc gactagggga
     6361 caagtaaaat ctgaaaaact tacctgagct agagtttcag ctagtttctg gctagcaggc
     6421 tcaaggagag cggtgtgaaa gggacctgac accttaagag gaatcaagcg tttggcacct
     6481 gcttcttgca aaagttcaac cgctcgatca actgcaacca cttctccagc aatgacgatt
     6541 tgtgcaggtg tgttatagtt ggctggagta accactccaa gttcagaagc tttttgacag
     6601 gcttcttcaa tgacctctac tggcgtattg agaactgcta ccatcttgcc agagtcagca
     6661 ggagccgctt cttccatata ggctccacgc ttagctacca aggcaaccgc atcttcaaaa
     6721 tccaaggcgc cgcttgccac caaggcagag tattctccaa gagacaaacc agcaaccata
     6781 tcaggctgat agcccttttc ttgcaataaa cggtagatag caaccgaagt cgctagaatg
     6841 gctggttgcg tatagcgggt ctgattgagt ttgtcttctt ccgtatcgat gagataacgt
     6901 aaatcataac cgagcacctg actcgctcga tcaatcgttt ctttgacaat cggatactga
     6961 tcatagaaat cccgtcccat ccctagatac tgggcacctt gaccagcaaa taaaaaggct
     7021 gttttagtca tttcttacaa ctcctgccca gcgagaggct tcttcttgaa ttttcttagc
     7081 ggctccgtaa tacaaatctt ttaggatttc ttcagctgtt tcttctttag aaacaagccc
     7141 tgcgatttga cctgccatga cagagccacc atccacatca ccgtgaacaa ctgctttggc
     7201 tagggcacct gctcccattt gttcaaagat ttctaaatca ggatcttcct gcttaaaggc
     7261 atctttttca gccagttcaa aatctctagt caactgattt ttaatagcac gaacagcatg
     7321 accaaagtgc tgagctgaaa tcgtagtatc aatatccctt gcttttaaaa ttttctcctt
     7381 gtagtttgga tgggcattcg actcttttgc aactacaaac cgtgtcccca cctgtacagc
     7441 ctctgcacct agcataaagc cagccgcagc accttcacca tccgcaattc ctcctgcagc
     7501 aataacagga atagatatag ctgtggctac ctgtcgcacc aaggtcatgg ttgttaattt
     7561 accgatatgc cccccagctt ccattccttc tgcaataaca gcgtctgcac cgattttttc
     7621 catgcgttta gctaaagcga cactaggaac aacaggaata acgattatcc cagcttcatg
     7681 gaaacgttcc atatacttgc ttggatttcc tgctcctgtt gtgacaactt taacaccttc
     7741 ttcaataacg agatccacga tatcttccac aaagggagat aagagcatga tgttgacccc
     7801 aaagggttta tcagtcaatg atttgatttt atcaatattg gccttgacaa cttctttcgg
     7861 ggcatttccc ccaccgataa ttcctaatcc tccagccttg gaaacagccc ctgccaaatc
     7921 accatcagca acccaggcca tccctccttg gaaaatagga tagtcaatct tcaataattc
     7981 tgtaatacgc gttttcatag tgcctccaac cttccttgct tacgtaatag ttcgatttca
     8041 ccataatttg acagtcaaac tattacctaa acaagaggga gtgggtttct ccctactcct
     8101 tctactaata ttctgcttat tttgcttgct cttcaacgta agcaaccaag tcaccaactg
     8161 ttttcaagtc attttctgct tcgatttgga tatcaaaagc atcttcgatt tctgagatta
     8221 cttggaacaa gtccaatgaa tctgcgtcca aatcatcaaa agttgattca agtgttactt
     8281 ctgatgcgtc ttttccaagt tcttcaacga taatttcttg tactttttca aatactgcca
     8341 tgataggact cctttaaaat aaatagtttt tttataacaa tgtgttcacc acatgattac
     8401 ctaaattgta agaatgagcg tgccccaggt caagcctcca ccgaagcctg atagaagaac
     8461 agtctggcta ccatctaaag ggatgagacc ttgttctaca cactctgaaa gtaaaatcgg
     8521 gatactggct gcactggtat tgccatattc catcatattg gctggaagtt tggctcggtc
     8581 aacaccaatt tttctagcca tcttatccaa aatacggtca ttggcttgat gaagtagcag
     8641 ataatccaag tctgtcacct ctataggaga ttcatcaata gtctgcttga tagacttggc
     8701 tacatctcga atggcaaaat caaagactgt gcgtccatcc atcttcaaaa acgaatctgc
     8761 actttcttga tctgaaaatg gagaatgcaa acctgaatgc ccataagtta aacactcgct
     8821 gcgacttcca tcgctattaa gactctcagc taagaaatgc tcttgctcgc tagcttctaa
     8881 caagacacca ccagcaccat ctccaaacaa cacagctgtt gatcgatccg accaatcgac
     8941 tgccttagag agggtttcac taccaatcac caagcctttt tgaaagcgac cagaagcgat
     9001 aaacttttca gcagttgaaa gagcaaatac aaatccactg caagccgcgg ttaagtcaaa
     9061 agcaaaggct ttattagcgc caatattagc ttgaacacga gcagctgtag agggcatcat
     9121 cgaatctgga gtaatggtag ctaggatgat aaaatccagt tcttttcctg ttattccagc
     9181 ttttgccatc agtttcttag caacctctgt agccaaatca ctggtagatt ctgttcttga
     9241 aatatgcctt tgtcgtattc ccgttcgact tgaaatccac tcatcattgg tatccataat
     9301 ctgagccaag tcgtgatttg taaccacttg ctctggcaca taatgagcaa cctgacttat
     9361 ttttgcaaaa gccattattt caaatcctcc aaaaattggt aaagattagt caagccttta
     9421 cccatgacag caatttcttc ctcgctcatg ccatcaataa ttttttctac catggccttg
     9481 tggaagcgtt tatgcagtct atgaatcaag cgacccttct ttgtcaaatg cagatgcacc
     9541 acacgacgat cctgttctga ccgaactcgc tcaatgtagc ccttacgctc taaattgttc
     9601 aaacttgtcg taacagttcc aagagttacc atcaactctt ttgacacttg acttggagtc
     9661 acatctggag ccttaccgat gacatcaatc gtatgcattt ctttgatgga gatatcctta
     9721 aaacgactac ctctcaagtt cacttcctca attacaagga cattgttaaa tatagatgtt
     9781 aaatattcat tgattcgttg gtagtccatt tttcatatcc ctccttcttc aaaaaacttt
     9841 tacaatcaaa acatttgaca gaaagaatta tatcaaaatt caaagaatga tgcaagtatt
     9901 ttttattttc ctgtaaattt aggtcttctt ctctccgaat gagctcgcac tccttctttg
     9961 aaatcctctg tttgagctaa ggatttctgt aggttcagtt ctaaagtagc gtaaccctgc
    10021 cagtctttaa attggctctc ccaaaccaac ttcttaatgg cagcatagga attacttgaa
    10081 gcacgtctta attttttaag aagctgttct ctcgtctttt caagtttttc agcttcagaa
    10141 acgcggtaaa ccaaccccca ttctagggct ttttctgccg ttaaagcctc tcctgtcata
    10201 gctaattgag cagcacgcgt cacaccgata ctgcggctca agagatgaat tccccctgca
    10261 tctggagcca aaccaacacc aacaaaggct tggataaact tagccttatc cgtcgccaaa
    10321 cagaaatctg ccgcaacagc catattcgct gcggcacctg caacagcacc gtcaacttcc
    10381 attaaaacag gtttggctat ttgcttgatt ttataagaaa tcgtattgac caactctgcg
    10441 atttttgtca atgatggaat atcatcctca tccactgccc gcttcatctc taccaaatct
    10501 cccccaactg agaagacctt tccattggca ttgattaaga taaaatgcac agctggattt
    10561 tcttctgcca aagtcagagc ttctaaaatc tcctcacaca tgggaatatg aaaaccattt
    10621 gcgacctcag gacggttcaa ggtaaggatt gccaagtcct cttcaaactg ataaataatg
    10681 tgttccatca gggctccttt ttattttata agcagtttat actcttcgaa aatctcttta
    10741 aaccgcgtca gcttccatct gcaacctcaa aacagtattt tgagctgact tcggctagct
    10801 tcctagtttg ctctttgatt ttcattgagt gttaaattat ttcactgtca aagtatatct
    10861 tttttaggag cagaaagcaa gaaaaaatcg actgaaagtg tctcagccga taagaaaggc
    10921 tcgctttagg ctagaaagca tcttattttc ttcattgaaa aaagcttttc tttctgctat
    10981 aatcgtataa aatacttact ttaggagttc ttatgaaagt tgttaaattt ggaggtagtt
    11041 ctcttgcctc tgctagtcaa ttagaaaaag ttttaaacat cgtcaaaagc gattcagagc
    11101 gtcgttttgt agtcgtttct gcgcctggta aacgcaatgc tgaagatact aaggttacgg
    11161 atgccctgat taaatactac cgcgactatg ttgcgggtaa cgatattagc aagagccaaa
    11221 gctggattat cgaccgctat gctgctatgg ttagtgaatt gggactaaaa ccagctgtgc
    11281 tagaaaaaat ttctaaaagc attcacgcct tggccactct tcctattgaa gaaaatgaat
    11341 ttctctacga tactttccta gcagccggtg aaaataacaa tgccaaattg attgctgcct
    11401 actttaacca aaatggtatc gatgcacgct atatgcaccc tagagaagct gggattgtgg
    11461 tcacaagtga acctggtcac gctcgcatca ttccatcaag ttatgacaag attgaagaat
    11521 tgacaaacac caatgaagtc cttgtcattc ctggtttctt tggtgtcact aaggaaaatc
    11581 aaatctgtac tttctcacgt ggaggttctg atattacagg ttctatcatt gctgctggtg
    11641 tcaaagctga cctctatgaa aactttacgg acgttgatgg tatctttgca gcccaccctg
    11701 gtattatcca ccaaccacac tcgattcctg agttgaccta ccgtgaaatg cgcgagttgg
    11761 cctatgcagg cttctcagtc cttcatgacg aggctcttct tcctgcctac cgtggaaaaa
    11821 ttcctctggt tatcaagaat accaacaacc ctgaccatcc aggtactcgt atcgttctaa
    11881 aacacagtaa tgatgaattt ccagttgtgg gaattgctgg tgactcaggc tttgtcagca
    11941 ttaacatgtc gaaatacctc atgaaccgtg aggttggatt tggccgcaag gttctgcaaa
    12001 tcctggaaga acttaacatc ggttgggaac atatgccaac aggtatcgac gatctttcta
    12061 tcattctccg ttctcgcgaa ctaactccca tcaaggaaga agaaatcctg cgtcagttgg
    12121 ttcaaaagac taaagtagat catgcagaaa tcgaacacga cctttctatc attatgattg
    12181 ttggtgaaaa aatgaagagc cacatcggag tgactgctac tgcgacacgc gctctatctg
    12241 aaaacaaaat caacatccag atgatgtctc aaggttctag tgaagtttct atcatgtttg
    12301 ttgtcaataa agaccaagag aaagcagcaa ttaaagccct ctacaatgcc ttttttggaa
    12361 aaagtaagga agactaagca tggcccaatc acttaacaaa acagtactcc tcagtacgac
    12421 aggcacttcc tacctctcta tcgctgggaa agttgggaaa ttccttgtcg gagatcaggc
    12481 tttggaattt tacccagatg tcaatgtcga acaatttatc cagattcctt ggagccatat
    12541 caatcagatt ggagccaatg tcactggacg caaaatcagc cgccacttcg aagtctttac
    12601 agacaaagga aaattcctct ttgcttcaaa agactcaggt gccattctca aaattgcacg
    12661 cgaaaaactc ggcaatgaca aggtcgtcaa acttccgacc ctgattcaga caattagcca
    12721 aaaatttaaa aatctatttg caaaaaagta gaaactttag tataatcata gcaacggata
    12781 agtaggttat cttcttcttt cagaaagtct gcgggtactg cgagcagata ggagggatag
    12841 gtgaaattct accgttagta acaattacat acacaatcaa gtgcacacct gtgaagttgg
    12901 atggaaccgt ggccctgcca ctccaacgtt ttgtcaggcg tgcttttttc ataaaggagt
    12961 tcttatgtta gatatcaaac gtattcgtac agattttgaa gctgtcgcag aaaaattagc
    13021 tacacgtggt gtagatgctg ctgtcttgaa tgaaatgaaa gaaatcgatg ctaaacgtcg
    13081 taacatcttg gtcaaagttg aaactctcaa agcagaacgt aacacagttt ctgctgagat
    13141 tgcccaagct aagcgcaaca aggaaaatac agatgacaag attgctgcca tgcaaaatct
    13201 atctgctgag gttaaagcct tggatgctga attggcagaa atcgatgcta aattgacaga
    13261 atttacaacg actcttccaa atatcccagc tgacagcgtt cctgttgggg ctgacgaaga
    13321 cgacaatgtg gaagttcgcc gttggggtac tccacgcgag tttgacttcg aacctaaagc
    13381 tcactgggat ctcggtgaag accttggtat ccttgactgg gaacgcggtg gtaaggtaac
    13441 aggcgctcgc ttcctcttct ataaaggcct cggtgctcgt ttggaacgtg ctatctacaa
    13501 ctttatgttg gatgaacatg gaaaagaggg ctatactgaa gtcatcacac cttacatagt
    13561 caaccatgat tctatgtttg gtactggtca gtatccaaaa tttaaggaag atacttttga
    13621 actcagcgat accaactttg tcttgattcc aactgctgaa gttcctctga caaactacta
    13681 ccgtgatgaa atcttagacg gcaaagatct tccaatctac ttcactgcta tgagtccgtc
    13741 attccgttct gaggctggtt ctgccggtcg tgatacgcgt ggcttgatcc gtttgcacca
    13801 attccacaag gttgaaatgg tcaaatttgc caaaccagaa gaatcttacg aagaattgga
    13861 aaaaatgaca gccaacgctg aaaacattct tcaaaaactc aaccttccat accgtgtcgt
    13921 tgctctctct actggagata tgggcttctc agctacgaag acttacgact tggaagtgtg
    13981 gattccagca caaaacaatt accgtgaaat ctcaagctgt tcaaacacag aagatttcca
    14041 agcccgtcgt gcccaaatcc gttaccgtga tgaagcagat ggcaaggtga aactccttca
    14101 taccttgaac ggttctggac ttgcagttgg acgtacagtg gctgcaattc ttgaaaatta
    14161 ccaaaatgaa gatggttctg tgaccatccc agaagcactt cgtccataca tgggtggagc
    14221 tgaagtcatc aaaccataaa aaataaggtt tagctatttc tagctagacc ttttttcgta
    14281 accaaatcag ataagcacct agtacaaaga ataaaatagt taggcatata atggtttcag
    14341 ccaataccag gtaatccaga aatggaagtt tcaaaattcc ctgagccatc ttgagcgagg
    14401 tcgctgtgat aatggttggg aaggtgaggg ctgagaaggc tggttgaaaa ccttgtttta
    14461 aaatgttggg cagacgagtt aaaacaaaga aaaagaagga ttgagaagcc aaaatcatga
    14521 caatcaagac ccaattcggc aggttggttc ctcctactcg aactagagaa gccaagagta
    14581 gagagaaagg agcacagtag attccttctt gtccaagcaa ggctagtggg agtggatgtt
    14641 tctttaaatc gctataaata aggggataga gatagaaggt caagagaaaa ccaaaactca
    14701 aggtcgcata ggcaatttcg ataataccta ccagaggata ggtcaaggca gccactgcta
    14761 tccccacata gagaaccgtc caacttggag tggcatgaac cctccgccct ggacaagcaa
    14821 acttgatggt aaaaccagca atcaaggtca aatccaagag aaatgaaaac caccaaatcc
    14881 cttgtgctac caaaggaaga taagagaata cgcgaaagac ataggtcgat aaaatcatcc
    14941 cagccatagg aaaggttgcc attcctgaca aaagaggggg cttggtcaat tcttgcttgg
    15001 tttctttcca attaaagaga tgcagaatta gaaagtaaat ccataaaacc aaaccagtca
    15061 gactaaaaag atgggataga accggcaacg tatctaaaat aagatttcca gctcctgcca
    15121 aacctagcaa acaacctgaa aatactaagg ggagtttttt catcctaacc tccaataatc
    15181 atgttagttt cagtataaca taaaagcgct taaatgatga tttaaaaaaa cgaagtccga
    15241 ttatttcaga cttcatttta ctcagatatg aattaggcat aaggttgcaa ttctggatta
    15301 attggtgtat tagctaagtt gttggcatag ttacagagga ttgctaggct gacaccaaaa
    15361 accacatcca aggcattttg ttgagtgtag ccagcttcta aaaactcaga caaggcttca
    15421 tctcctacac gacccttggt attgataact gccaaggtaa acttagctag ggtatccaat
    15481 ttaggatctg tttcaattgg agtacgattg cgaagagctt gaatcaagtc atcattcatc
    15541 tggatttgtt tgatggaaaa ggctgtgtga cctgcgacac agaaggcaca accattggtc
    15601 acggctgccg tgatttgcac cacttcacgc tcaacgggtg tcaggctgtt gcgacggtgg
    15661 atagatgaga caatttggta ggcttctaaa acagtcgggg cattggccaa gagaccgatt
    15721 aggttgggaa tatagccatt gttgtctttt tctactgttt caagaatttc tttcacttct
    15781 gctggtgctg actctactgt atggatagta aatgttgtca taagatacct cttttcttat
    15841 tattgacact aatattattg gaaaatctta taaaatcctg attcctaagt ttatctaaga
    15901 taaagcttta ttctctcata agattttcgt tgttatatta gtttatcaca cttccaatca
    15961 cttgcataat atatattata tatcaggctg ataaaaatta tttataggca aaaaaatcac
    16021 acgagctgtg tgattccatt atttgtcaaa atacttttta gtttcagcaa taacgacttg
    16081 cgacaagacc aagagggcaa tcaagtttgg cagagccatc aaggcgttaa cgatatctgc
    16141 gataatccag accatatcca actcgataaa tcctcctaac aagaccatga gcacaaaaac
    16201 cacacggtag agccagataa agcgaacccc aaagaggaac tcaaaacagc gttctccgta
    16261 atagttccaa cctagaatcg ttgtaaaggc aaaaagcaca aggaagatgg tcaagagggc
    16321 aggcccaaag tgtgaaaaga ctgttgagaa agctgactga gtcaaggcaa ccccattcaa
    16381 gtcaccactc caaactccag ttaccaagat ggtcaaacca gttagagtac aaatgatgag
    16441 ggtatcaata aaggttcctg tcatggaaat caaaccttgc tctactggtt catttgtctt
    16501 ggcagctgca gctgcaatag gagcagaacc cagaccagat tcgtttgaaa acacaccacg
    16561 cgccacacca ttttgaatag ccatccgaac gctagcacca gcaaatccac ctaccgcagc
    16621 aaggggacta aaagctgagg taaagactaa agcgattgtg ccagggattt ttccgatatt
    16681 aaagaaaata actgtaagag ttcctaagat ataaatgatg gccataaaag gaacaacagt
    16741 agttgaaacc ttagaaatag acttgagtcc accaaagact gcaatcgcta caaagacaga
    16801 caagacgaga gctgtgatgg ctggcgaaat cgtcgttgta ttttggatag attctgtaat
    16861 cgagttgact tgggtgaagg ttccgattcc caagagagca accaatactc ctgctactgc
    16921 aaacaaaaca gcaagtggtc gccacttttc tcccatccct agaaggatat aatgcatggg
    16981 acctcccgct actgcaccat ggtcgtcctt ggtgcggtat ttgatggcca agagtccttc
    17041 cgcatacttg gtagccattc caaagaaagc cgccatccac atccaaaata gagctcctgg
    17101 tccaccaacc ttgatagccg tcgcaactcc tatgatattt cctgttccaa cagttgatgc
    17161 caaggctgta cacagagctg taaaactgga tacatcacca tgtcccttat cctggataaa
    17221 aataagctga aaggccttgg gcagacgcaa aacctgcaag agtcctagcc gcatggttag
    17281 gtaaatccct gttccgacca ataaaatcaa gaggggcggt ccccaagcaa aagcatcgat
    17341 tgatttaagc aattctaaca tttccttctc ctatcgtttc aaccccaaaa gaaagagcac
    17401 atgcaagata catgtactct ggaatgctta gataaatgct aaaaagcggt ctatcctagc
    17461 tctgtccttt tacctgagag tttgagcagt tgcctgcctt gccccttcgg tgcctttacg
    17521 gtctctccag agttccgtcc atttacagtc atggaaaatc aaacgattcc ccacttctat
    17581 taaacttcat tcggtgttgg tatttaattg attctaattt tacaaaaaat gttggctttt
    17641 gtcaatgtgt ttattagtaa aaattagttc aacagttttt actttataaa gtccagaata
    17701 ctgctatcct ttaaaagtga caatagtcgc accactgcct ccagcatttt gtggggcata
    17761 gccgaaactc ttgacatgtt tgtttctttg caagtatttg gtaactcctt cacggatgac
    17821 tcctgttccg ataccatgga tgatatcaac ttgagccata ttgttaagca aggcttggtc
    17881 gatgaaggta tctagctcat tcatggcttc ttcatagcgc ttgcctcgaa gatccagtct
    17941 agcttgaggt cctcgcccag aagttcgttt cacaacattg acctgtttct tcttgactgg
    18001 tttttcttgc tgggcttgaa caagatcaaa ctctttctct tccaaggtca tcttaatcaa
    18061 gccaacttgg gcttcccagc ggccgtcctt gagttgactg gtcaaggtac cacgctgacc
    18121 ataactgaga accacgatat catctcccac ctttggagct cgttttttct tggccttttg
    18181 aaggaccttg tttttagaca agtccacttt ttcaggagcc aattttttca acttggcttt
    18241 ggcttcaatg atttcgtggg gcttgagttg ggatttactg tggagatttt tgagaatctg
    18301 gtcactttca cttagggcca tatccacaat ctcagcagcc tgttcacgcg ccttgttaag
    18361 ctcggtttcc ttttcacgat taagctcgtt gtagagtttt tttagcacac ggttcatctt
    18421 gagattttct tgctccacct cacggatatt gtccaagcgt ttgcggcttt ccagcgtctg
    18481 ctcttctagt tgctcaatga tacgattgac gtcattgtcc tgatcgatct gctgactggc
    18541 atctcctacg ataacttcag ataggcctag acgtttggca atttcaaagg cattacttcg
    18601 gccaggaaca ccctgcataa agcgataggt cgggcgaaga gttgcagtat caaactccat
    18661 actggcattt tgcacaaagg ctgtctcaat accgtaggcc ttgagttctg gatagtgggt
    18721 cgtcgccatg gtcttgattt gacgcaggcg aaggtcctcc agaatagcca tggcaagggc
    18781 tgctccctct tggggatcag taccagcccc caactcatcc aaaagtaaga gtgaatgttg
    18841 gttgaccttg ccaagaatat ccacgatatt ggtcatatgg ctagagaagg tagacaagct
    18901 ctgctcaata gactgctcat ctccaatatc agcaaagatt tcttcaaaaa taccaacacg
    18961 acttcccttg tctgctaaaa tcggcaatcc tgactgggcc atgacctgtg tcaagcccag
    19021 agttttgagc atgatggtct tcccacctgt attgggacct gtaatgacaa tagctgttaa
    19081 atcttgacca aaatagacat catttgcgac ggcatttttg accaaaggat ggcagacatg
    19141 gagcagttga atctcttgat tttctgacag ctgaggcacg actgcttgtc tttcttggat
    19201 aaatcgaacc ttggcacgaa tcaagtccag atgaccgata atccaagcgt cattagcaat
    19261 ctcagccgca tgagggcgga cacgctcaga aatttcttgg agaatgcgaa gcatttcata
    19321 gcgctcatct gctcgcagac tagcaatttc ttcgctcagt ttgactacct cacgtggttc
    19381 gatatagacg gtgtttccac tagcagaaat atcatgaacg acacctgcaa tcttattgcg
    19441 gtaggtgttt ttgactggta aaacctgacg gccatttctg ctagcaacaa ttccttccgt
    19501 caacatctgc gctttttgct tgagcaagtc ttgtaaaaca tcgcgtacct gactctcgct
    19561 atcatgtatt tttcgacgga ttcgcgccaa ttcttcactg gcaaaatttt caatgaaacc
    19621 cgcatcatta aaggcctgaa gatttccttg taattgcgga aaatcatgta atttctcaaa
    19681 ccaaagggct aattcttcca agctgacatt ttccagattg gcgtaaaaac tttgaagttc
    19741 tcggctggta agaagcacgc gtttcaagag taggaactcc tcgatattga gatccgctcc
    19801 catctccaac ctcttgcaga ctcctgcaat ttccttagtt gagagaatag taaaatgcgg
    19861 ttgctcgacg aaaagagcct gcatttcttt catctcagca aaagcctgtt tgattttatc
    19921 tgctttggca gtcggagcca gctgtctcaa ttgctccaag ccctgctcgg tcaacaaatg
    19981 aggctcaaac aaggccttga ccttatcgaa ctctaatgtt tctaatattt tcttattcat
    20041 ctttatttcc ttgtctttga atgtctaggt gtggtagctt tttacattct gatagattcg
    20101 agtcaaatct gtaaacaaag ggctaggaaa actcctgccc tttttatccg attaaatttg
    20161 tcacccagat ttgtttgagc caactggttg ttatcggtat gctctggatg atgtgttttg
    20221 cgacgatact cttttcaaga ggattttgta taactgccat ggggatggtc gccaagattg
    20281 tcaaggccat ttgcaagaca aataaggtca ccaacatgga caagatacct gctgaaactt
    20341 ggaacaactt accacccagt tttttgctag gaatcaagtg taagagaaga ccaagcaaac
    20401 gaccaatgct atagacaatc ccaaatacaa gcaagtagcc gatacctgca taaaagacct
    20461 tatccagctg aaagagttga tccgatggga aaaagaaagt cccctgacct tcctgcgaat
    20521 ttgcataagg gagcaataaa tggaattgct ctccaagccc cttataaaac tggccagcca
    20581 taaaagccga tgccatggct gaaatcaggt aataaacctg taagagcagg cctctccgat
    20641 agccgatata aaatccccaa accaagacca atagaagaag gaatgaaatc ataaggaatc
    20701 ctcaatcttg ctctgttctt gcttacaagt cacaagcttg tgacggagtt cttctagctc
    20761 ttgctcctta tcgtcaaatt caatctcacg gctgagctga gttgataaac agttgactgc
    20821 caacaaaaga gcgattgttt catcatctgc gctaggcatt tgttctttaa ttgcttggta
    20881 tttttctgtc gcaaccttag cgatttcctc cataaaaagg ttatcatgtt cgcttgtcaa
    20941 ggttaacgat tttttcccga atgtaaattt gaatcgattt agatttgcca taaaattcac
    21001 ctcacgatat tataccaaaa ttcgctaatt ttgtcagttt ttacaaattt tactgctttt
    21061 gtggtacaat agaaactatg gcaagtatca cactcacacc aagcgaaaag gatattcagg
    21121 cttttcttga acactatcaa accagtctgg ctcctagcaa gaatccctat atccgctact
    21181 ttttgaaact acctcaagca acggtttcta tctatacttc tggaaaaatc ttgcttcagg
    21241 gtgaaggggc tgaaaaatac gccagtttct ttggctatca agctgtagag caaaccagcg
    21301 gacaaaatct tcctttaatt gggacagatg aggtgggaaa tggttcctac tttggtggac
    21361 ttgcagttgt ggctgccttt gtcacacctg accagcacga ctttttacga aaactcggtg
    21421 tgggggattc taagactctg accgaccaaa agatccgtca gattgctcct attctcaagg
    21481 aaaaaattca gcaccaggca ctccttctct cacccagcaa gtacaacgag gtcatcggag
    21541 accgctacaa tgctgtttcg gttaaggttg ccctccataa tcaggctatc tatctcctcc
    21601 ttcaaaaagg tgttcagcct gagaaaattg tgattgatgc ctttaccagt gctaaaaatt
    21661 atgacaagta cttggcacaa gaggccaatc gtttcagcaa tcctatcagc ttagaagaaa
    21721 aggctgaggg caaatacttg tctgtcgcag tttcttctgt cattgcgcgt gatctctttc
    21781 tggaaaatct tgaaaatctg ggacgagaac tgggttatca gcttccaagt ggagctggaa
    21841 cggcttctga caaggtggct agccagattt tgcaagccta tggtatgcag ggactcaact
    21901 tctgcgccaa attgcacttt aaaaacactg aaaaagcgaa aaaacgctta gaaaggtaag
    21961 ttatgaattc atttaaaaat ttcttaaaag agtggggact gttcctccta attctgtcat
    22021 tactagcttt aagtcgtatc tttttttgga gcaatgttcg cgtagaagga cattccatgg
    22081 atccgaccct agcggatggc gaaattctct tcgttgtaaa acaccttcct attgaccgtt
    22141 ttgatatcgt ggtggcccat gaggaagatg gcaataagga catcgtcaag cgcgtgattg
    22201 gaatgcctgg cgacaccatt cgttacgaaa atgataaact ctacatcaat gacaaagaaa
    22261 cggacgagcc ttatctagca gactatatca aacgcttcaa ggatgacaaa ctccaaagca
    22321 cttactcagg caagggcttt gaaggaaata aaggaacttt ctttagaagt atcgctcaaa
    22381 aagcccaagc cttcacagtt gatgtcaact acaacaccaa ctttagcttt actgttccag
    22441 aaggagaata ccttctcctc ggagatgacc gcttggtttc gagcgacagc cgccacgtag
    22501 gtaccttcaa agcaaaagat atcacagggg aagctaaatt ccgcttctgg ccaatcaccc
    22561 gtatcggaac attttaagaa acctaagagg ccgagaatca ccaatctcag cctcttcttc
    22621 tatcgtgaga aaatgattgg tactatctaa acttaccaga acagaaacac ctcaactctc
    22681 acctattcat gcaaaggaat tttatggaag tttatttttc aggaactatt gaacggatta
    22741 tttttgaaaa tcccagcaat ttttatcgca tcctcctcct agaaatcgac gatacggacg
    22801 cagaggattt tgatgatttt gaaatcattg tcacaggaac catggctgat gtaattgagg
    22861 gcgaagacta tactttttgg gggcaaattg tccagcactc caagtatgga gaacaactgc
    22921 aaatcagtcg ttatgatcgc gcaaaaccaa ctagtaaggg cttggtcaag tacttttcaa
    22981 gtagccattt caaggggatt ggtctcaaga cagctcagaa aatcgtggat acctatggcg
    23041 aaaataccat tgacgaaatt ttgcaacacc cagaaaagtt agaaggcatc gcaggactct
    23101 ctgccaaaaa tcgcgaggct ttcgtctcca ctctccgtct caactacgga acggagatga
    23161 ttttggccaa actagccaac tacggcattc ccaacaaact agcctttcag attcaagact
    23221 tttacaagga agaaaccctt gatgtggttg aaaattatcc ctaccaactg gttgaggata
    23281 tcaagggttt gggctttacc attgctgacc aactagctga ggaactaggc atcgaaagtc
    23341 aggctcctga acgcttccgc gccggtctag ttcacagtct ttttcagacc tgtatggaaa
    23401 caggggacac ctatgttgaa gcacgggatt tgctggaaca aacccttact ctccttgagt
    23461 cttcccgtcc cgtggaacta gaccccagcc aagttgccca agagctctcc tacctgatcg
    23521 aagaagacaa ggttcagcag attgatacca agatctttga caacagcctc tttttcgctg
    23581 aggaaggtat ccgcagtcac ttgattcgta tccttgaaaa aggaaaacag aagagccaag
    23641 atttagaaac tattcaaaag catatcacta ctgtcgagca agaactgggg attgagtatg
    23701 ataacattca aaaacaagct atttgtgacg ctatccagaa caaggtcttt atcctgacag
    23761 gtgggcctgg tactggtaag acaactgtta tcaatggaat catcgctgtt tatgcccttt
    23821 tagaaggact tgacttcagg aagaaaaaca atctgcccat tcttcttgct gctccaactg
    23881 gtcgagctgc tcgtcgcatg aatgaattga caggtttgcc tagcgcgacc atacatcgcc
    23941 acttgggaat gacaggtgac gatgatacca gtcatctgga agattaccta gatgctgact
    24001 ttatcatcgt ggatgaattc tctatggtgg atacttggct ggccaatcaa ctcttctcca
    24061 atatctcttc taacagtaag atcctcatcg taggtgacag tgatcagcta ccttctgtca
    24121 gtcctggaca ggttctagcg gatctgcttc atattccttt gattcctcag actcgcttgg
    24181 aaaaaattta tcgacaaagc aaagaatcaa ccatcgtcac cctagctagt cagattcgac
    24241 agggcatctt gccagctgat ttcacccaaa aaaaagctga ccgttcctac tttgaaattg
    24301 ctagtggcca tattcctgcc accattgaaa aaatcttagg tgccgccctc agaaatggta
    24361 ttcctgcccg tgatatccaa gttctggctc ctatgtaccg agggacggca gggattgatg
    24421 ctatcaatca gctcatgcaa gacctgctca atcccccaca aaaagatcaa ctcagttttg
    24481 aagctcctca gtgccactat cgtaagagag acaaggtcat tcatttggtt aacgatgctg
    24541 aaatcaatgt ctttaatggg gatttaggag ctatcacaga cctgattcct ggtaaataca
    24601 ccgaatcgaa acaagacgag attgtcattg attttgatgg taatgaagtc tcttaccccc
    24661 gtaacgaatg gtacaagatt cgcttggcct atgccatgag tattcataag tctcagggaa
    24721 gtgagttccc agttgtcatc ctaccgatta ctagtgctag ccgacgtatg ctggagcgaa
    24781 atctcatcta cacagccatt acacgtgcca aaagcaaact catcttacta ggcgaattac
    24841 aagccttcga ctatgctacc caacacatcg gaactgcccg aaaaacctat ctgattgaac
    24901 gcttcagtga tctactggag aatgttgaag aaaagcaaca agctgtctct gaaacagtca
    24961 catcaagtgc ctctgaacaa tcctacatcc taaccgaaga aaactgggac cgcatcccag
    25021 ccatgattgg gattacagac acagacctca aagagatttt tggaaaatag tgcatcagaa
    25081 aacccaccaa ttcaggtggg ttttctgttt attaagatta ttttactgtt gctgtgcttg
    25141 taatcaattc aacagctttt ttgattgtga tatcatgttt caacatgtca gctgaaagca
    25201 agctttgtac ttgagcaact tccatgttgt agtctgctgc caattgctca acttcttttt
    25261 ggatttcttc ttctgaagca tcaaatcctt cagctttggc aactgcttcg ataacaaggt
    25321 tagtcttagt acgtgactca gcttctgctt ggtattggtt gtgaaggtct tcttgagtag
    25381 ttccagtgat ttggaagtac atgtcagggt tgatcccttg acgttgcaag ttcccaagga
    25441 attcatttac tgaacggtga acttcttcgt ggatcatttc ttctggaagt tctacgattt
    25501 cagcattttc tacagctgta tcaattgctg caccttcaac tgcatctttg taagcttctt
    25561 ctttagcagc agccaattct ttgcggtatt tttctttcaa gtcagcaagt gtttcaactt
    25621 cttcatcaat gtcttttgca agttcatcgt caagagctgg aacttcttta gcttttactt
    25681 catggatagt tgtcacgaat ttagcttctt tacctgcaag gtcttctgct tggtagtctt
    25741 ctgggaatgt tacgataaca tcaacggttt cgccagctga gtgacctacc aattggtctt
    25801 cgaaaccagg gatgaattga cctgaaccaa gtccaagtga gaagttttca ccttttccgc
    25861 cgtcaaattc aacaccgtcg atagaaccaa cgaagtcgat cacaacagtg tcgccgtttt
    25921 cagcagcagc ttccttgata accaattcag tcaggttgtt gcgttcgcgt tcgatacgct
    25981 cttcaacatc agcgtcagtt acttcttttt ctacatcaac tgatacttca aggtttttgt
    26041 agtcacccaa ttttacttca ggttttgtaa cgacttcagc agcgataacc cagtcttgac
    26101 ctttttccat tgaagttacg tcaatttttg gttgggcaac cacttcaaga ccagcttctt
    26161 ttacagctgc ttcataagcg tttggcaaaa gagcgttcat aacgtcttgg taaagtgatt
    26221 cttcaccaaa ttttttatcg aagataggac gtggaaggtg acctttacgg aaacctggaa
    26281 cattaagaga tttcttcact gacttgaaga cacggtccaa ttctggtttg atttggtctt
    26341 gagagatagt gaaagtcaag acaccacggt ttgtttcttt gttttcaaat gatacagaca
    26401 ttctgtcatt tctccttaaa attttttaaa tacagtctat tataacattt tctataaata
    26461 aaatctagac taatgcagcc aattatagac aacaagcctt tttcctaact aaaacactca
    26521 taaagtatag acaattttag actactttgg atatcttttg gttagtcatt ttccaaaact
    26581 aatttatcta atttttcagt tatatttgac ttcatcttct cagtaacatg agtataaaca
    26641 cttaaaatct ttgtttcatt agtataatct acacgatcca ttatcgctgt tataggtact
    26701 tccaattcag caagcaaact tatatgcgaa tatctaaaaa catgagcagt caatacaaaa
    26761 tcatctttct taatttcact tttcctaaaa ctgtttttca aaaagtaatt gaaacaagtt
    26821 atcattaagg gagaaccatt atttgtaaat atataatcac cttcttctaa aagaagatat
    26881 tcttttaaaa tttctaacac tctatcacta ataccaatta ctctatatga agctaaactt
    26941 ttcggactat ccaaaaatac ttcgttattg gacgattaca ttatagtaaa acgaaataag
    27001 aacaggacaa atcgatcagg acagtcaaat caatttctaa caatgtttta gaagtagagg
    27061 tgtactatta tagtttcaat ctactataca agacgaaatt gaagaaatct ttccattata
    27121 aaacgcatat tatcaaggtt tttaaacacc taataatatg cgttttttcc tattttaaag
    27181 acttttatcc acctattata caaagttaag tatccatcac agtttacaat tagccttcat
    27241 tacctaaccc attcaccatc cttcaagaga gtaaccttta agaaccgaat aaataaatga
    27301 caaatttcgt tcatttaatt taactgcact gttaatttga ctagctccat ctcctaaagt
    27361 atgcactcct actacgcggt gactagcatc ataaactgta gatccactag acccctctaa
    27421 agtatcaact tggtaatcca agaacatgcc atcatcactt aaaacttgtt ttttatctgt
    27481 atacatttga tgaattttaa aattatatga tggatagcct gtgatagtca cagttattcc
    27541 tgtcaaattt ttttgactag taggaagacc caaagtccct aattttgcac caatgggctc
    27601 ttctagaatt aataaagcca agtcatattc ccttgcatcc ttagaattta aatttctaaa
    27661 ttccttcaaa taacaaactt cctttacttt gatctttcca aatagttctt gacttggact
    27721 aacagccgga agaacataaa tatcatccgc ttctttccca tagtcatgtc tgtagtagtt
    27781 gtgagccgct gtaattaaca cattatcttt aataaaattc gcacttcccc tcccttctac
    27841 attcattcca tcggctactc cataatactt agataaaacc atagctgaag tcgaatacgg
    27901 atactgtaaa gtattatcaa ttttaatcaa atcatcatta ccgataatac ttctgattgc
    27961 ttttggtagt atgaaccata cgttggtgaa atctcagata atgaagaatc attagactct
    28021 ggaccttttt ctagtgtctc acttacctca tattcttcac ctgtactaga aataacactc
    28081 aaagcagata ctgtcgataa ctggctagcc aataaagtac tcgcaataat tgaaataccc
    28141 aattttttat aaacagtttt cttcattatt gtatcctcct aatgtaatta tagcgtacta
    28201 ttctaaattt cttaatctac tatagaatca agaaatctac caccttcttt aaataccctc
    28261 cattatcaca taaacaggta aacttttcaa ttaatgactg cgcttttcaa tcacgctaga
    28321 ggtacttgct tgcttctttg atactaagtt cagccattct ttccttgttt ttctcaataa
    28381 agcatgttac ccaagtggga ttcgttttgg agtagtctcg cagagtccag ccaatggctt
    28441 tattgataaa aaattctgtt tggttcaagt tatgaagtag aatcttttcc attaattgag
    28501 tattggtctt ctcttttctt aacaactggt ggtcaatagc gacacgtctc agccagatat
    28561 tatctgatag gctccatttt atactcaatg aaaatcaaag agcaaactag gaagctagcc
    28621 gcagattgct caaaacactg ttttgaggtt gcagatagag ctgacgtggt ttgaagagat
    28681 tttcgaagag tattaagatt atttcttcta gttcagagtg ttcatacacc aaactcccta
    28741 ctactcgatc taggatatct accgtgtccc accaggattt tgtcacgact aactgctcta
    28801 gcttaggcaa atcggtttcc tttagataag actgcattgc tttcaaatag ttagcagcca
    28861 catattggta ttctctagga tccttttccc agcaagtgtc tgcaaaatcc caatcgataa
    28921 tctttgtttt tttcgcttct ggaaaatatt tttatagagt ttatttcttt caggcaccgc
    28981 aatacctaga aaagaaaatt gatggcgcat ataggcttcc atggaccttg cttttttaga
    29041 gtcttttgct gcttctagct cctcaagtaa atctgctaaa ctcatctaaa actcctcttg
    29101 ccccaccaaa tggtgctgaa aggcatagac agtcgcctgg gtacgatcgc tgacttcaag
    29161 tttggcaaga atattggaca cgtgggtctt gaccgtcttg agagagataa agaggtcatc
    29221 tgcgatgcgc tgattttcgt agcccttggc gatgagttgg agaacatctc gctcacgcgc
    29281 agtcaattct tcatgaagtt ccatatgatt gcggtggtat tcaaccttct tgctaacctc
    29341 ttgctcaatg gccagctcgc cagcagctac cttactgacg gcatgaagca attcatctgc
    29401 actagaagtc ttgagcatat agcctttggc accagcatct aagactggca tgattttttc
    29461 attgtccaaa taagaggtca caatcaaaat cttggcttca ggccattctt taaggattgc
    29521 taaggtcgcg tcaatcccat tcatctcagg catgacaata tccatgacaa tgacatctgg
    29581 acgcagttcc aaggccaagt caatcccttg agacccgttg gacgcctcac ccacaacttc
    29641 tacatcgtct tggaggtcaa agtagctttt caagcccaat cggaccattt catggtcatc
    29701 tactagtaaa attttcatct ttactccttt atcattcctt atctaacagg ggaatacgga
    29761 tatcaactgc cagcccttgc ttgggagctg ttaataactg aaccgtccct gccatatctt
    29821 caacccgctc cttgatattt cgcagtccat aactcaagtc gtctaagctc cctaaccgga
    29881 aaccaatccc attgtccacc accttcagtt gcaattcaac atctgtctga tagaggtaga
    29941 catctaggca agatgcctgg gcatggcgga gcgtattgct aatcaactct tgcaggatac
    30001 ggaagatatg ctcctcgatt ttcttaggca atttcgtcat attctgcttg agactaaccc
    30061 taagatcact cttgtcctca agctctttta agagaatctg aatcccttct atcaagctct
    30121 tctgctccag ttcaactggt cgcaaatgca agagcaaaac ccgcaaatcc ttctgggcag
    30181 tttctaaaat agctgtgaca ctctgcaact ggatctgcat cttttctcta tccaatttca
    30241 aagcctgctg actgataccc gataaaatca tgtgggccgc aaacaactcc tgactgactg
    30301 tatcgtgcaa atcccgagca attcgcttcc gttctttctc gatgatttcc tcttcctgag
    30361 caaggctatg attttcagct ttttgaagag cttctgtcaa aaggttaagt ttacctgata
    30421 aggacttgaa actggcatcc aaatctggat ctgcaacctg aaccacttct tgccctgcca
    30481 ataaacgctt gagattagcc tgcatttttc ttagagaaag ctcttcgatc cctcgccaaa
    30541 acagggctaa gagacaggtt atggacatgc tgaaaaccaa caataaaaag acaaattttt
    30601 ctgttttttc gacatcgtgc aaaaagatag accagtcaaa atcaagtatt tccagcaagc
    30661 tgtgggagaa aaaaaagaca aataggaagg aggtgagagc aataatgaca taggcttgtt
    30721 ttttcatcct ctaaccacct ccacatcacc aatcatagtg gtcaagaaaa tcttgacact
    30781 cttgttactc ttgagatagt cttttgtttc ttgatgatag tgttcattgc ggagggctcg
    30841 cttgggctgg ttgaaaaaag tcaaatcccc atagagacag ttaacgctga gactgacttc
    30901 cacatctaca ggtacgatga ttttggtcgt tcctaccatc tttctgagga taatgacatt
    30961 gtcatgattg gttaagatga ccctctccag atgaatagtg tccttgccca tgaagcgaaa
    31021 gagattgata tcatcgaatt ggcaagtctg gtagcttgaa aaatgatgaa gatttccaaa
    31081 ccaacgattt ttctccttct taaccgtcac gacctcttca aaaaccaaat tggtctgctc
    31141 tttttcctgg ttcatcatcg ggtaaagaag aaagaggcta tagataaccg caacaaaaat
    31201 agctagaatc acaaaaggat tgagcataac gatgaaaaag aagagaatgg ttgccgctac
    31261 taaaagaaga ttatttccct ctttaccagt gtagtagcga atcaaaagca aaaagaggaa
    31321 tagtatcagc agaaaacgcg aaaaatgctc tgataccatc aaaatcagag ctcctgtcag
    31381 aagacaggct tcgataaata aaaagatttt aaattttctc ataggttcat cctctccctt
    31441 ctattttatc acaattcaaa aaagtcacct cagtctgagg atggaaaaaa aggcgctggt
    31501 tacgcctttt tcatctgatc ctttgcttct tttaattttc cataaagaag ataatctact
    31561 ttttgtagat ctgctatggt ggcacagtta agggaacaca taatcaagcg tagatctgct
    31621 ttccagcctt ggacaatgcc aatcacttct tcaactgtgt aggtttcaac caattccaga
    31681 acggttcgtg acaatcccac agccttagca ccaaaaacca agcacttaat catatccagc
    31741 ggattccgaa cccctccact aaccaagagt tcgaccttat ctttccattc ttgggcattg
    31801 agaagggcct gcatggtaga ctgaccccat tgattgaggt aatcacgctg gccactacga
    31861 cggttttcga tataggcaaa gctggtgcca ccacgacccg ataggtccac tgtacgaaca
    31921 ccgaattcat aggctctttc gattgtcttg gcatccattc caaagcccac ttccttgagg
    31981 acaataggaa cgggaatttg cttgatataa tctgctaaat gcgattgcca gcttctaaac
    32041 ttcctttctc cctcgggcat gagtaattcc tgcatgacat tgacatgcac ttgcaataga
    32101 acaggattca tctcttctac agtctgaagt cctaactcga caggcttgtc caatccaata
    32161 ttggttccaa ggaggagatt gggatgacta gacttgacag aaaaagaatc atccgttgga
    32221 tttttgaggg ctgcgctata agaacccgtt acaaataaaa taccacagga ttccgccacc
    32281 tgagccagct tttgattgat ttctcttccc ttattacttc caccagtcat ggcattgata
    32341 taaaaaggaa agtcccactt tcgaccagca aactctgtcg aaagatcgat ttcatccaga
    32401 ttgtaaagag gcaaggaaga atgaatcagc tccacctcat caaagctatt ataggaactt
    32461 ttctgctcaa gggcatagag gatatgctcg tccttacgat ttgtcgtcat gtcctatcct
    32521 ttcttgatat aagagctcaa tccccagatc ggcccaacga ttttttaagg tttcggttga
    32581 ttgcgcatca aaactcaggg cgatgccaca gtcaccacca ccagcaccac tactcttggc
    32641 aacggcctgc aaatcttgac tggcttcttt caactgtcta agcaaaggcg tgtaaatatc
    32701 tgtactcaag ccttctaaaa gcttgctggc tacttctact tgctcgataa ctttttcggc
    32761 tttcccctgc tccaaggctt cgaccaaaga aaccaccgtt tcttttgagg aacttaaaaa
    32821 attttgattg atattttgct tgatttgctg gaccatgtga ctcgatacag ccacttcctt
    32881 ggtccatccc actaagaaat cacattctaa agttggtttc acttgtgaga taaaaaatcc
    32941 ccaatcacgc tccagaactg tcgccaagtt ttcttcttct aaccaagcag ccgccttctg
    33001 gcgatcaaat gactggtaga gaaccaaatc ctctgccaca atacaggcaa ggtcgcccat
    33061 ggaaccattg tctcctcgct tgagcaagac agcgctagtc agcttgaaca agagattctg
    33121 atcaaccgaa agattataga gagccagtaa agccttgaca accaagacaa cgacgctgcc
    33181 actagaacct agaccaaact ttttcccttc tcgttccatt ttgccacaga tttttaggga
    33241 aaaaggtctt aaattctgac cgcgaacagc gaggaagtct cccatcaaag caatcgtttc
    33301 ttgaatcaag ctgtagtcag gattgggcct taagtccact gcgaaatcaa acatatctga
    33361 atagatacgg tagctgtcag aaaaagcaat ctcagccctc atatagatgg gaatatcctt
    33421 tatcaaagct aactgccctg gctctaaaat agcatattca cctgcccaat agagttttcc
    33481 gcaagtttta acagcaatca tcttgactca aatcctttgt ttttgacaca atcaagcgat
    33541 aacgctgacc gaaaatttct gacaaatgct ccaagtcttt ctcctgacag aagaccttaa
    33601 cattgggacc agcatccatg gtaaagtagc aggcctctcc tttctcacga agctggcgaa
    33661 caaaggccat agcctcataa gaggcatccg tcagataaga aaaggctgga ctagcagtct
    33721 ttgtcgtagc atgcatagcc agagcatttt tctccgttaa ttctccaatc ttggcaaaat
    33781 cattttcctt gagataaatc agcatatcct gatagtcctt ctcagactga cgaacccagt
    33841 cgtcaaaagt cgtcgaggtt tccacacaaa gtttcatccc gtcacggcta gagattggtt
    33901 ttttcttgtc ctctagcacc aacataatca tagctagttt caagtctgtc tctacagggt
    33961 aaatttctcc actatcctta tcccaggctc ctagtggtcc ataaaaactc cgagaagaag
    34021 agcctgaggc aaatttggct tcctgtgcca actgacttct atccaatcca agcttgaaat
    34081 aagcattaca agccttgacc agggcggaca aaccactaga acttgaggac aggcccgctg
    34141 ccgtaggcat attgttttga gtatcgatac ggacaaagcc ctcaccagct ggacgataac
    34201 ggtcaataat cttactcatc ttggcatgct cgacctcatt ttgtagctga ccattgatgt
    34261 aaaattcgtc agctgttaca ttggctggta aaggcgacaa ggtcgtctct gtatacatat
    34321 tttccaaagt tagagaaata ctgctagtag caggcaccat ctctttttct tttttctttc
    34381 cccaatattt gataatagca atatttgcgt aggaacgtac tgttacaggc tctctatcca
    34441 tgtctgaaca gctcctttct cttctaatct ttctgctagt tcttgtgcgt gcgtcaaatt
    34501 ggttaccaag gctatgatac aacctcctag cccaccaccg ctcatcttgg cacccagagc
    34561 accatagcta agagccgttt caaccaaaaa gtctgcctca gggctactga ctccaatttc
    34621 ttttagatgt aaatgcgctt gactgaggat ttgtcccagt ccttcagcat ctttttgtga
    34681 aatcgcaact tctgcttgct gggttaattc tcccaaggca tgcaaaaacg gtagggcatc
    34741 cttgccctta ttttgaacca cttggatggc ttcacgagta tgaccataaa cacccgtatc
    34801 ggcaatcacc aaataggcgg ataaatccat ctcaagttct gtaaatccta cgttcttgat
    34861 aaagcgaata ggttggtcac tgagacaggt cttagcatcc aaaccactag gattcatatg
    34921 ggcaatcatc tcagctcgat tgaccaagat ttctagtaca tcatgaggca gatcagcctg
    34981 atagtagtca aataccgcac gaatggccgc tatgctgata gccgctgacg aacccatccc
    35041 ccgtttctca gggatagccg agtcaatctc acaacgaacg caggcttctg tgatatccaa
    35101 atactccagt gaggcataaa ccgccatgga caaggtatcc tcctcataaa ggcgccaagg
    35161 actctctgca gaaactacct tacaggtcac ctccacctcc aaaagaggca gggaaatggc
    35221 aggataaccg taaacgaccg catgttcccc tattaaaatt atcttactat gtgcctgacc
    35281 gacaccaact ttttttgtca ttttttcctt ttactagacg aaaaaacgtc ttatttttca
    35341 tacaagtatt aattctttcc tatctatttt attatatttt cacaaaaaaa gcgattgttt
    35401 ccattcacaa tcgcttcttt cattattgaa cccattcgcc attataattg acaaaatagc
    35461 catctacggt cgtattcact gccaaggcac ctgagctata agcgtagtac catctgccat
    35521 tgacctggaa ccaacctgtc gtcatagaac gacgaaagaa actccatacc attaagtaaa
    35581 gaggaaagtc gtgagggagc atgcgccatt gacaacctgt tttagtgacg tacaaagtct
    35641 cattaacaag tactcgtttc ggccatttat aggtgcggtg tttggagaaa tagggttcaa
    35701 tcttagccca ttcttgatcg tttaaatcag tatcatatac tttgcgtgtc ataactctag
    35761 tttaacattt tttgtgaata caggttctaa ataatcgacc acgaaaattt cttaagtgga
    35821 aaacgcctta tgaagtatgc tacgggaaag ttatgcactt aatttgacaa ttcaagatgt
    35881 aaaaatatat actatagta
//
LOCUS       DAVKAO010000013        35672 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKAO010000013 DAVKAO010000000
VERSION     DAVKAO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.013"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(288..1921)
                     /locus_tag="VWX16_000686"
                     /pseudo
     CDS             complement(288..1921)
                     /locus_tag="VWX16_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1902..2651)
                     /locus_tag="VWX16_000687"
     CDS             complement(1902..2651)
                     /locus_tag="VWX16_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158076.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIVSSY
                     DLSSSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            2831..4285
                     /locus_tag="VWX16_000688"
     CDS             2831..4285
                     /locus_tag="VWX16_000688"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9158077.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAEDAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHMEAFQVIEVDPVDRTVKLGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKSVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4375..6993)
                     /gene="alaS"
                     /locus_tag="VWX16_000689"
     CDS             complement(4375..6993)
                     /gene="alaS"
                     /locus_tag="VWX16_000689"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9158078.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESAFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELALIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(7015..7500)
                     /locus_tag="VWX16_000690"
     CDS             complement(7015..7500)
                     /locus_tag="VWX16_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9158079.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEILESQS
                     H"
     gene            complement(7775..8014)
                     /locus_tag="VWX16_000691"
     CDS             complement(7775..8014)
                     /locus_tag="VWX16_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158080.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8275..9345)
                     /locus_tag="VWX16_000692"
     CDS             complement(8275..9345)
                     /locus_tag="VWX16_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9158081.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKELQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKKLLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9342..10115)
                     /locus_tag="VWX16_000693"
     CDS             complement(9342..10115)
                     /locus_tag="VWX16_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158082.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(10112..10918)
                     /locus_tag="VWX16_000694"
     CDS             complement(10112..10918)
                     /locus_tag="VWX16_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158083.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10899..12056)
                     /locus_tag="VWX16_000695"
     CDS             complement(10899..12056)
                     /locus_tag="VWX16_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158084.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12115..13065)
                     /gene="murB"
                     /locus_tag="VWX16_000696"
     CDS             complement(12115..13065)
                     /gene="murB"
                     /locus_tag="VWX16_000696"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9158085.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(13177..13934)
                     /locus_tag="VWX16_000697"
                     /pseudo
     CDS             complement(13177..13934)
                     /locus_tag="VWX16_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
     gene            complement(13944..14675)
                     /gene="budA"
                     /locus_tag="VWX16_000698"
     CDS             complement(13944..14675)
                     /gene="budA"
                     /locus_tag="VWX16_000698"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9158086.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14817..16088
                     /locus_tag="VWX16_000699"
     CDS             14817..16088
                     /locus_tag="VWX16_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9158087.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKLSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16108..16923)
                     /locus_tag="VWX16_000700"
     CDS             complement(16108..16923)
                     /locus_tag="VWX16_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9158088.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTNLKSANIAAQKGT
                     VPESMVKEQLPKVQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(17080..17733)
                     /gene="phoU"
                     /locus_tag="VWX16_000701"
     CDS             complement(17080..17733)
                     /gene="phoU"
                     /locus_tag="VWX16_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9158089.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17745..18503)
                     /gene="pstB"
                     /locus_tag="VWX16_000702"
     CDS             complement(17745..18503)
                     /gene="pstB"
                     /locus_tag="VWX16_000702"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9158090.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            complement(18516..19319)
                     /gene="pstB"
                     /locus_tag="VWX16_000703"
     CDS             complement(18516..19319)
                     /gene="pstB"
                     /locus_tag="VWX16_000703"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9158091.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLSQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19330..20214)
                     /gene="pstA"
                     /locus_tag="VWX16_000704"
     CDS             complement(19330..20214)
                     /gene="pstA"
                     /locus_tag="VWX16_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9158092.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20204..21121)
                     /gene="pstC"
                     /locus_tag="VWX16_000705"
     CDS             complement(20204..21121)
                     /gene="pstC"
                     /locus_tag="VWX16_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9158093.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(21121..21999)
                     /gene="pstS"
                     /locus_tag="VWX16_000706"
     CDS             complement(21121..21999)
                     /gene="pstS"
                     /locus_tag="VWX16_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9158094.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22573..23877)
                     /locus_tag="VWX16_000707"
     CDS             complement(22573..23877)
                     /locus_tag="VWX16_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9158095.1"
                     /translation="MKFPEGFVEKYKEILGEEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKKPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLQETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23878..24651)
                     /locus_tag="VWX16_000708"
     CDS             complement(23878..24651)
                     /locus_tag="VWX16_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9158096.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24641..24919)
                     /locus_tag="VWX16_000709"
     CDS             complement(24641..24919)
                     /locus_tag="VWX16_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9158097.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24919..25320)
                     /locus_tag="VWX16_000710"
     CDS             complement(24919..25320)
                     /locus_tag="VWX16_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9158098.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25530..26147)
                     /locus_tag="VWX16_000711"
     CDS             complement(25530..26147)
                     /locus_tag="VWX16_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9158099.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(26137..26910)
                     /locus_tag="VWX16_000712"
     CDS             complement(26137..26910)
                     /locus_tag="VWX16_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9158100.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26921..27658)
                     /locus_tag="VWX16_000713"
     CDS             complement(26921..27658)
                     /locus_tag="VWX16_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9158101.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(27663..28793)
                     /locus_tag="VWX16_000714"
     CDS             complement(27663..28793)
                     /locus_tag="VWX16_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9158102.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28877..29266)
                     /locus_tag="VWX16_000715"
     CDS             complement(28877..29266)
                     /locus_tag="VWX16_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9158103.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29282..29665)
                     /locus_tag="VWX16_000716"
     CDS             complement(29282..29665)
                     /locus_tag="VWX16_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9158104.1"
                     /translation="MLEVAYILVALAVIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29666..30454)
                     /locus_tag="VWX16_000717"
     CDS             complement(29666..30454)
                     /locus_tag="VWX16_000717"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9158105.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30447..31382)
                     /locus_tag="VWX16_000718"
     CDS             complement(30447..31382)
                     /locus_tag="VWX16_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9158106.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31512..31688)
                     /locus_tag="VWX16_000719"
     CDS             complement(31512..31688)
                     /locus_tag="VWX16_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9158107.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31828..32535)
                     /locus_tag="VWX16_000720"
     CDS             complement(31828..32535)
                     /locus_tag="VWX16_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9158108.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32686..33714
                     /gene="queA"
                     /locus_tag="VWX16_000721"
     CDS             32686..33714
                     /gene="queA"
                     /locus_tag="VWX16_000721"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9158109.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34375..35502)
                     /locus_tag="VWX16_000722"
     CDS             complement(34375..35502)
                     /locus_tag="VWX16_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9158110.1"
                     /translation="MNTSFAHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRFEKDETKYRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVIVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNTSGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT        10662 a         8115 c         6738 g        10157 t
ORIGIN      
        1 aataattata atttcaccta tctgtatcat tctatttcga actctaaata tatgttctat
       61 caaaaatact tggatcacac acattatagg aattaacgtt tttgaaattg aaaaatatcc
      121 caataaataa actataaaca acaaaaatag aactatgtta tatttcttat tcaaaacatt
      181 cctccctata tatttttgat taccaatctt aatcatttac aactacattc taacaaacta
      241 taaaagcgtt tgtcgaattg aatttatcaa gcaagcgacc aaccagctca tcttttttct
      301 atttctgcca atatgcgtga caggtaataa tgatagccaa aaatagcaag agcaagcagg
      361 aggataagag ctcctactcc caagctgatg gcaaggatag gggagagaga ctgaaccaag
      421 aatatactcc caattacaag ggccatcagg attgcactat aaataaacaa taaaactatg
      481 gcgactatgc catttgaacg attcaccagg tccgtaatgc tactccaatt ggttgacaga
      541 tttttcacat ccttaaaata atggtgacaa gaaaggatga cactggcaag gatccatgcc
      601 acaataaggt aaatcatcga aaggatgggc aagcctagat atagagaaag accaagtaaa
      661 gtcagaactg gtaaaaagga ctgaacagca aagataatcc aaaatttcac tttcacataa
      721 cgagcaaagt caaagggtaa actcttcaga aaatcaacat tttccctctc caaggacaag
      781 gcaattgaag gcaggctggt gatattgtta ttgacaactg ctataaagag agctataaaa
      841 aaaagggtaa ccagtatgga ggatgaatgt ctggaactat ctgagaatct cggattttgg
      901 aaatcagacc gatcatcatg agataaggaa ggaaagcact tgtaaaaagc actgtaacca
      961 cgccagtccc ctgtcccaag agggtgaggt ggtagcgtaa aaccatgcgg aaaaatccct
     1021 ttttagtggt tgaaattctc tccttgctgc gacgttcttt tttgaccttc tcctcactat
     1081 taagcaggat cacgtcataa aaacgaggaa ggaccttctt tttggtcaga taaagcagga
     1141 agagagttag tcctatccaa gcgagcagac ccaatatggc ttctattgaa aaaggctcca
     1201 ctgctatttt gtaaaagata tgaagaggat aaaggagaaa tggaatgtct ctaactttgt
     1261 caacaatact tccaaaagtc gactgaagaa agaagataaa tattaaaggt atgagaactc
     1321 ctatcccaat catcacattc gaaaaaatag actgatactt tctgaagacc ctagtctgag
     1381 ccaagaaatg tactgccact accgtcacta aagtaacaga gacaaataat aaggtcaagg
     1441 acagtagcat caaaggcaaa cccagccaaa gagaaggagc tagactaata tagagggcta
     1501 gaaaataagc taggattggt acaattccag ttagagctgg caagaggaca gacagtcctt
     1561 tagcaattat aatctctgat tctttaaaag catagggact ataggaggcc aggtccttac
     1621 tctcataaaa gacattgtaa aatgcagtta aagaagttga aaaggcaatc actagtaaaa
     1681 tagcaaccac gaaactaaaa taaacaggca tttcctcaaa aggaaaatga atggctatag
     1741 tactaaaaaa gatgagcatc aagagactgg aaaaaatgta agaacttagg actctagcgg
     1801 aaacatttac tttttttcca gtatttttag cttgcttctt tcgtaggtta gccagattag
     1861 cttctttaga tgaatagagg atattgatat caactaattt tttaatgacc ttgagacgca
     1921 tctgaaacct cctcttttct accagcaagg ctaaggtaga tactttccaa agactggtct
     1981 gggttgtctt ttctcaagtc ctctacctta ccacaataaa tcaaatgccc ctttttcaaa
     2041 atagcaatcc gatcacagac ttgctcagct acctctagca catgagttga aaacaaaact
     2101 gtcttacctt tttgcgcatg ctccttcatc atttgcttca aatcaaaggc agcctgggga
     2161 tccaaaccag tcaagggttc gtccaagacc caaatatcag gatcagacaa gagtgccccg
     2221 atgacaaaaa ctttctgacg cattccatga gaaagggttt caataacctg ataacgattt
     2281 tcagcaaaat caaaaacgtt caataaccta gctaaactag cttccaagtc agagctgctc
     2341 aaatcatagg atgaaacaat caattcccaa aattcattgg ccgttaaacg taaaaataag
     2401 tcaggcgaat ctgctacgta gccaatcttt cgtttgatcg ccaagcgatt ttccgataac
     2461 tcctgaccat ctaccaaaat acgaccactg ctgggtgaaa tgatactgac tagggatttt
     2521 atggtggtcg attttccagc cccattatga ccaatcaagc ccataatctc tccattttca
     2581 atctgcaaat tgagattact caaggcctct ttatcaccat acaacttact aacattttga
     2641 aattcaatca tgggatgact cctatcttta tctatattaa atactattat actagcactt
     2701 tgatacaaaa aaatcaacac tttattttaa gtagatacaa tagtttcctt ctattcttac
     2761 gcaaacgttt gcgctaacca atactttatg atagaataaa gaacaagatt gacaagtaag
     2821 aggaaaaatc atgcaaaatc aaacgctcat gcaatacttt gaatggtatc tgccccacga
     2881 cggtcagcac tggacgcgtc tggctgaaga tgctccacac ctagctcatc tggggattag
     2941 ccacgtctgg atgccaccag cttttaaggc aaccaatgaa aaagatgtcg gctatggtgt
     3001 ctatgactta tttgacctag gagagtttaa ccaaaaaggg actgtccgca ccaagtatgg
     3061 tttcaaagaa gactatcttc aagccattca agccctaaaa gcacagggaa ttcaacctat
     3121 ggccgatgtg gttctcaatc acaaggctgc agccgatcac atggaagcct ttcaggttat
     3181 cgaagtggat cctgtagacc gtacggttaa acttggagaa cccttcacca tcaatggctg
     3241 gactagcttt accttcgatg gtcgccaaga tacctacaat gacttccact ggcattggta
     3301 ccacttcacc ggtacagact acgatgcaaa acgccgtaag tctgggattt atctcatcca
     3361 aggggacaac aagggctggg ctaacgagga attggtcgat aacgaaaacg gaaactacga
     3421 ctacctcatg tatgccgacc tagactttaa acatcctgaa gtcatccaaa acatctatga
     3481 ctgggctgat tggttcatgg aaacgactgg tgtagctggt ttccgtttgg atgccgttaa
     3541 gcatattgac tctttcttta tgcgcaactt catccgcgat atgaaggaaa aatacggtga
     3601 cgatttctat gtttttggtg aattttggaa cccagacaag gaagccaatc tggactatct
     3661 cgaaaaaacg gaagaacact ttgaccttgt cgatgttcgt ctccaccaga atctctttga
     3721 agccagtcaa gctggcgcaa actatgacct tcgtggcatt ttcacagata gcctggttga
     3781 actcaagcct gacaagtctg tgacttttgt cgacaaccac gatacccaac gaggacaagc
     3841 ccttgagtct accgttgaag aatggttcaa gccagcagcc tatgccctca ttttgttacg
     3901 ccaagacggc cttccatgtg tcttttacgg agactactat gggatttcag gccagtatgc
     3961 tcaacaagat ttcaaagaaa tccttgaccg cctcctagcc atccgaaaag atttggccta
     4021 tggagaacaa aatgactact ttgaccatgc taactgtatc ggttgggtac gttcaggtgc
     4081 tgaaaatcaa tccccaatcg cagtcttgat ttcaaatgac caagaaaaca gcaagtcaat
     4141 gtttgtcggt caagaatgga ctaatcaaac ctttgtagat ttacttggta accaccaagg
     4201 tcaagttaca attgatgagg aaggttatgg acaattccct gtctcagcta gatccgtaag
     4261 tgtctgggca gtcaatacca tctaatagct cataataacc aagctaggtc caagcggatt
     4321 tggctttttt gtattcacaa aaagacctac ccaaatggat agatctttac ttgattacaa
     4381 tttacctgct actgcatcca acaattcttg gatcttagct tggttgcttc ctcctgccat
     4441 ggccatgtct ggtttaccac caccacgtcc atcgatgatt agtgctaatt ctttgacaag
     4501 gtttcctgca tgaaggtctt ttgtcttgct tgctacaagg acattgactt tgtcaccgat
     4561 agcggcaact aggacaagaa gatcagagta gtctttttgt ttccagttat ctgcaaaagt
     4621 acgaagggca ccggcatcgg atacagacac ttgactagca atgtaacgat gaccgttgac
     4681 ttccttaaca tctttgaaga tatcgcctgc ggctgcagct gcggcttttt ctttcaactc
     4741 agcattttct ttttgaagtt gacgaagttg ttcttgaagt ccttctacct tgtgaggtac
     4801 ttccttgact tgaggtgctt tcaaggttgc tgcgacagct ttaagagcat cctcttgttc
     4861 acgataggct tcaaaggctt ccttaccagt cactgccaag atacggcgag ttcctgaacc
     4921 gattccttct tctttgacaa ttttgaagag accaatctca gaagtgttgc caacatgagt
     4981 accaccacaa agttcaatag agtagtcacc gatagtcacg acacgaactt ccttgccgta
     5041 tttctcacca aagagggcca tagctcccat ttctttagca gtgtcaatat ccgtttcaac
     5101 tgtcttaact tcaagagctt cccagatttt ttcgttgact tgctgttcaa tcgcacgcaa
     5161 ttcttcagca gttacagctt ggaagtgggt aaagtcaaag cgaaggaatt caacttcgtt
     5221 aagagatcct gcctgtgttg cgtggtttcc aaggatattg tgaagggcag cgtgaagcaa
     5281 atgagtcgca gtgtggtttt tcatgacacg gtgacggcga ttgctatcaa ttgccaaggt
     5341 atattcttgg ttcaaggcaa gcggtgcaag gacttcaact gtatgaaggg cttgaccatt
     5401 tggggctttc tgaacattgg tcacagtagc cacaacctta cctgactcat ccaagatttg
     5461 tccgtagtca gctacctgtc cacccatttc agcataaaat gacgtttccg caaagataag
     5521 agaggcagtt ccttctgaaa cagcttctac ttctgcattg tccgccacga tagctaccaa
     5581 tttagaagac aattggctag cattgtagtt gaaggcactt tctacagtga tgttttgaag
     5641 agtttcattt tgcataccca ttgagccacc cttgacagct gacgcacgcg cgcgttcttg
     5701 ttgttctttc atggctgctt caaaaccttc acggtctaca gtcataccag cttcttcagc
     5761 gatttcttca gtcaattcaa ctgggaaccc ataagtatca tagagtttga agacatctga
     5821 accagcgata acagattgac ctttttcttt caagtctgct acaatgcctt gggcaaagtg
     5881 ttgacctgag tgaagggtac gggcaaatga ttcttcttcg ctcttaacga ttttctcaat
     5941 aaagtcacgt ttctcaagca cttctgggta gtagctttcc atgatttttc caacagttgg
     6001 aacgagtttg taaaggaaag gctcgttgat acccaatttt tgaccatgca tagaagcacg
     6061 acggagaaga cgacgaagga cataaccacg accctcattt cctggaaggg caccatcacc
     6121 gatggcaaat gaaagtgaac ggatgtggtc agcgatgacc ttgaagctca tgttgtcgcc
     6181 atcttggtca taaaccttac cagacaattt ctcgacttca cggataatcg gcatgaagag
     6241 gtccgtttca aagttggtct tagccccttg gataacggcc accaaacgct ccaaaccagc
     6301 gcccgtatca atgttcttat gtggcaattc cttgtattcg ctacgaggaa cagcagggtc
     6361 tgcgttaaat tgtgacaaaa cgatgttcca gatttcaata taacggtcgt tttcaatatc
     6421 ttctgcaagc aggcgaagac cgatattttc tgggtcaaag gcttccccac ggtcaaagaa
     6481 gatttctgta tctggtccag aaggtcccgc accgatttcc cagaagttgt cctcaattgg
     6541 aatcaagtga cttggatcca ctcccacttc aatccagcgg ttgtaagaat ctttatcgtc
     6601 tggatagtag gtcatgtaaa gtttttcagc agggaaatca aaccattcag ggcttgtcaa
     6661 aagctcataa gcccaagtga tagcttcgtc acggaagtaa tccccgatag agaagttccc
     6721 caacatttca aacatggtat ggtgacgcgc agtcttccct acgttttcga tgtcgttggt
     6781 acggatagcc ttttgggcat tggtaatacg tggattttca gggataatgg tcccgtcaaa
     6841 gtatttctta agggttgcta ccccagagtt gatccacaaa agagttgggt catttacagg
     6901 aaccaaactt actgatggtt ctactgagtg acctttggtc gcccagaaat caagccacat
     6961 ttggcgtact tgtgcactag atagttgttt catattgtct ccttattcac ttgtttaatg
     7021 tgattggctt tccagtattt ccacatagtc aatcgcgaca cagagggaaa tgactaggtc
     7081 tgcataagcg tcttcaagaa ccgttacggt ataggtagag gtcagatgga agagttcctt
     7141 cttaatttcc gcaatcaact gatcgcgatc atccagcaat ttgaaattca aatcccagat
     7201 attgccctcg atacgaagac ctagattatc aaactcatac ttatctcgcc aaaaggtcaa
     7261 cttcttacga atgacaaaac tcgagccatc ccgaagctga atctcaaaac gaggaagcaa
     7321 ggtcaagatt tctttactga tctgactgac ttgttcacca gccgcatcat agatggtaaa
     7381 agttttggga atcttaaaaa atgatccctc cacctgatag gcaatttctc ccctgtcatc
     7441 cttgatagcg aagcgttcgc ctccaagacg aaacttttgt ttgacaagaa atgttttcat
     7501 caacacctcc aaaaatcaaa agacaagctc atatcacgaa gggcgaaaaa ccgcggtacc
     7561 accttcattc aatgaacttg tcattctctt gttcttatgc aattgtatga ttgagtagca
     7621 tgacttccta gcttagatgg ctcgcagcac cgccatttct ctggactaag acaagtgaaa
     7681 atcaattctc aactttctta ttataacgtt tttttaagct tgcgtcaact ggaaatgatc
     7741 tccgttgaat tagaccaatt ccctacatct ctgattactt tttcaggata tattttttct
     7801 tactgccgtt tttcttttta tcccaaattt tcatattact aaacacagct actagaatat
     7861 ttccaaatat aaaggtgcct atcacccaat atatggactc agttgttagg tattgtcgat
     7921 ccaagccatc ctttaaatgg aatagtataa cagtttggtt aacaatcata aaggttggcc
     7981 agaaactttt tttgaaaaaa gtagacattt tcattatttg ttgccgcttt ctgtaaggtt
     8041 aatactcaat aaaaatcaaa aagcaaacta ggaagctagc ctcaagctgt acttgagtac
     8101 ggcaaggcaa cgctgacgtg gtttgaagag tataggctaa gtatactact aggcaagcaa
     8161 ataagcaaat aagcaaataa acaactagaa tagaaaaaga tagggctcta aaaactgact
     8221 tctattcctt aaaaacgaac cagcttgact gattcgtctt cttacgttta tctcctactt
     8281 ccgatacatt ttaaactgta ggaagaggtc gctatatttc cctgtccatt tatggtcaaa
     8341 tttctcataa acttctaggt gtttcatggt ttcaacatcg ggatagaagg ccttatcttc
     8401 ctttgtttcc tctgggagca atttcttcgc tggtaggttt ggtgttgaat agccgacata
     8461 ctccgcattt tggagagcat tttcaggttt caacataaag ttgataaagg cataggctga
     8521 gttttggttt ttaactgttt tgggaatgac catattgtca aaccaaagat tgctggcctc
     8581 tgtcggtacc acataacgta gattttcatt tttttctaac atttggctgg cttcaccaga
     8641 gaaggtcacg ccgattgcaa cattattctg aatcatatag cccttcatct cgtccgcaac
     8701 gatagccttg atatttggag tcagtttgta gagcttatcc actgtctctt ccaactgctg
     8761 caattccttg gagttgaggc tgtagccgag ggaattgagc cccagaccca gcacctcacg
     8821 cgccccatca aagagcatga tagaattctt atactccggc ttccaaaggt catcccaatg
     8881 ctcaggcgct tcatctacca tggtttcgtt gtagacaatt cctaaggttc cccagaagta
     8941 agggatggag aatttattac ctgggtcaaa ggactggttg agaaactctg gtccgatatt
     9001 ttcgattcct tcaatttttg aataatcaag cggaaccaag aggtcttcgt ccttcatctt
     9061 gttaatcatg tattcacttg gaatggcaat atcgtaggtc gttccaccct gctttatctt
     9121 agtgtacatg gcttcgttgg agtcaaaagt ctcgtactga acttgaattc ctgtttcttc
     9181 tgtaaactga gtcaagagtt caggatcgat atagtctccc cagttataga taaccaattt
     9241 ttgactatct cgactattga ttttactatc taaatgagtc gcaattcccc acaagacaag
     9301 gataatcgct gcaattcctg ctaaaaatga atagattttt ttcatgcttg ctcctccttc
     9361 tcacgagaga taaagtaata acctacaact aggataatac taaagagaaa gactagagca
     9421 gacagggcat tgatttctaa ggaaatccct ttccgtgcac gagagtaaat ctcgactgac
     9481 agggttgaaa agccatttcc tgttacaaag aaggtcacgg caaaatcatc cagcgaatag
     9541 gtgaaggcca tgaagtaacc tgcaatgata gacggagtca ggtaaggaag catgatttcc
     9601 ttaaacatct gaaattgact agctcccaag tcataggccg catgaatcat gtcgccattc
     9661 atttccttga gtcgaggcaa aaccatcaag accacgatag gaatggagaa ggccacgtga
     9721 ctagatagaa cggtcaaaaa gccaagtgaa aacttgagtt gggtaaagag aatcaagaag
     9781 ctagcaccaa tcataacgtc aggcgcaacc atgaggatat tattgagtga tagaaaggct
     9841 tcttggtatt tcttacgaga ctggtagatg taaatggcac caaaagtccc gataatggtc
     9901 gctatcaagg ctgataggaa ggccaagaaa aaggtctgag ccaaaatcag catgagtctc
     9961 ccatctccaa acatggtttc aaagtgagtc cagctaaaac ctgtaaagct attcatatca
    10021 tcaccagcat taaaggcata gccaatcaag taaaagatag gcaggtagag gaccagaaag
    10081 accagtccca gataaaggtt ggcaaatttt ttcatcgttc tctcctttcc ttagtcaccc
    10141 acatggtgat gaacatggtc aggatgagaa tcacaccgat ggttgaaccc ataccatagt
    10201 tgtcattggt tagaaaattc tgctcaatag ccgtccccaa ggtgataacg cggttcccac
    10261 caatcaaacg ggtcagcatg aagagactca aacttgggat aaagaccgac tgaaccccac
    10321 ttcgcacacc gttcatagat agagggaaga tgacatgacg gaaggtctcc cacttagttg
    10381 caccaaggtc atagctggca ttgatgagat tattatccat atcgtctaag acattgaaaa
    10441 tcggcaaaat cataaagggg agctcgatgt agcttgcgac aaagataaag gaaaaatcgg
    10501 taaaaagcaa ctgttgtgaa ccaattccga taaattccaa gaattggtta atagagccat
    10561 tttgaccaaa aatcccgata aaagcatagg ctttaaggag caaattgatc caggtaggaa
    10621 ggataatcag catgagccag agttgacggt gcttgagacg ggtcaaaaag agggctgttg
    10681 gatagctgat aagcaaggtc acaaaggtca caatccctgc ataaagcact gagttgaaac
    10741 tcattttgag gtaggtcaag ttttgtgacg caaagtaaga tttataattt tctaaactaa
    10801 actggccttc aatgttgaaa aaggattgtc cgaaaatcaa gaccaagggt gccaatacaa
    10861 agagggcaat ccaaagcatg tagggcacta caaagagttt agagcttgtt ttcttcatct
    10921 ctttcctcct cgatcgcatt gatcagacct gcttcttgct cttcgatttc cacgtattct
    10981 tcgatacgag catcgaactc ttcttcggtt tcattgagac gcatgatgtg gatgtcttct
    11041 ggttcaaagt ccagaccgat ttcctcaccc acgatggcct tacgggttga gtggatcatc
    11101 cattcatttc caagttcgtc ataggcgata atttcataat gaactccacg gaagagctgg
    11161 gtgtcgacct taacttggag tttgccttct tcaggaaggg taatgcgcaa gtcctctgga
    11221 cgaataacga cctcaacagg ttcatttggc ttcatcccac catcaaccgc ttcaaagcgt
    11281 ttgccgttaa attcgaccaa gtagtcctca atcatggtac ctggcaagat gtttgactcc
    11341 ccgataaaag tggcaacaaa gtggttgatt ggctcatcgt agatgtccac aggggttcca
    11401 gactggacaa tctcgccatc attcataacg aaaatccagt cactcatggc aagagcttct
    11461 tcctgatcgt gagtgacaaa gacaaaggta atgcccaatc gttgttgtaa ttcacgcaat
    11521 tcgtactgca tgtctgttct caatttcaag tccagcgctg ataaaggctc gtccaacaag
    11581 accacacggg gttggttgat gatagcacgg gcgatggcca cacgctgacg ttgtcctcca
    11641 gaaagtttgc ggatggaacg tttttcataa ccttccaact gaaccatctt gagaacttcc
    11701 gctacacgct gctcaatttc tttcttatca attttacgca agcgaagtgg aaaggcaaca
    11761 ttttcaaaca cattcatatg tgggaacaag gcataggatt ggaagacggt atgtacgtcg
    11821 cgcttgttgg ttggaatatc attgatacga acaccgtcta gcatgatatc tcctgtcgtc
    11881 gcatccagta aacctgcaat aatgtttagg atagttgatt tccccgaacc agatgcacct
    11941 agaagggtgt agaatttccc ttcttccaac tcaaagttga tgtctttgag aaccttggtg
    12001 ttgctgtctt caaaaacttt agagacgttt ttgaattcga taattggttt tttcaattgg
    12061 cataaattcc ttctttttca tagattaacc gatcggggct ctgtcaggtc cccactacct
    12121 cttgcaggga gtaaaaccac ctgcatacat cttcgctacc gataggcttt cacccaagat
    12181 ccggacttct ctttcaagcg taatacctga gtgttccttg actttttcga taaccgattg
    12241 gatcaagtcc tcgtagtctt tggccgttcc atctgcgaca ttgatcataa atcctgcatg
    12301 cttttctgac acttctacgc caccgatacg atagcctttc aagccagctt ctgaaattaa
    12361 ctgacctgca aaatgcccga ctggacgctt aaagaccgag ccacaagatg ggtattccaa
    12421 aggttgcttg agttcacgta ggtgcgtcaa gcggtccatt tcctgcttga taacctgatg
    12481 ggttcctgga gctagggcaa atttaactga caagacaact gcaccagact cctgaatagc
    12541 tgaatggcgg taaccaaaag ccaagtcttt agcagacagg gtttcgattt ctccatcctt
    12601 ggtcaagacc ttacaagact gcaagatgtg agcaatctcg ccaccatagg cacccgcatt
    12661 cataaagaca gcaccgccaa cgcttcctgg aataccacaa gcaaactcaa agccagttaa
    12721 actatgacgg agggcaatgc gagttgtttc aatcaagtta gccccagctt ctgcttcaat
    12781 ggtatagcca tcaacagaaa cgttattgag cttgtcacac aagatgacaa atccacgaat
    12841 cccaccatca cgaacgatga tattgcttgc attgccaaga accatccaag ggatattttc
    12901 ttggttggca aatttcacaa cgcgagccaa ctcaaaacga tttcgtggaa agaccaaata
    12961 atcagcctct ccacctactt ttgtataact atagctatgc aagggttcct taaaacggat
    13021 atcaattcct tctaagattt caagcatttt ttctcttaca gacatgtcac tcttcctttt
    13081 acaaaattca ttccattata ccatttttag agacatttga cgaccataaa aataccttgt
    13141 ttggattttg cataagaaaa agaggttccc ccctttttat gattttttac aaaagatttc
    13201 cttggttcca taggcgacca gaacgagctc cagtgctaga atcacttcaa ccaagactgg
    13261 atttgtcaac cagcctactt ggaaaagaga tggtgccaga tcaaagaagg catgcaagcc
    13321 ataggctgct aggagataaa tccatttctt ctggcgaaca gcttggtaaa cccaaactgt
    13381 caaaagtaat tggaaaccaa gcgccaagat tcgctcaaaa ccaagcaaat aaatctgcca
    13441 gaccgaaagt gactgaatgg tttttaacat attttcagac agtaattgca taacctgtgc
    13501 tgtggattct gagtttgaac tgccgaaaga acgatgtaaa gattgagtaa actagtaagg
    13561 cctagaaaaa tcaactccaa gccaccatgc cctaggccat aggccaaggc atctgccttt
    13621 tccaaacttc tctttttctc caaccatttg aagaaaacaa gacgagccgt ttcctcaaaa
    13681 aatgctgcca tggctaggcc atagataata tagataagcg gatgatcttg caagagggca
    13741 atactaccgt ctttttgagg gtgcaaaatc aagatatgca ccagtttttc caaaatctgc
    13801 gaagagacaa agaaagcgac tgcacccaag cccaagacag ctagattaat ctgatatttc
    13861 tttttggcaa accaaatgct tcctatcaga aaagctagca acagcaacat ggtaataatg
    13921 atatgaatgg tcattttctt ctcctattct gccttttcaa tatctttttt catctcgtca
    13981 acattgaact tagcaaacaa gtattgacgg tcttggactg ggaaacgttg gtccaactgg
    14041 tcaactgctc ccacctcgat aatgccttcc ttgatgacaa aatccatgac atgtccaccg
    14101 aaggtcaaat catctgatat gaagtgcaga tggtagcctg ccacactgac cccatggaaa
    14161 atctcaggcg tccagaaacc aacaatagtc cccgcaacat tgtcacgact atattccggt
    14221 tgatgggttg cgacatcagc aaacttggta tcgggtgttg acttaggaat catacgcaca
    14281 tgcatatgta aaaattcccc ccgaatcttg atagagcgga aaagattttc cccatcataa
    14341 taagactcaa ttcgttcttt caattccttg tctgtcatct caaagcgctg gcgaaaaatg
    14401 acctctgcct gatgcggtac cactgcagcg taaggaataa gggcatctgg tgacacttcc
    14461 acaatttctg gctggtctcc tgaccctttg gcctgataag ccttgccatc caagacaatc
    14521 aattccccat caatggaatc caaggttccc aaaccaaggt caccatgttc tagcaattct
    14581 cctactgtca tagtaccacc ataaaggcca gccatcaagg ctccaagagt attgtattga
    14641 aataatttca ctggttcctg cacgttctta tccattcact ttcttgtctg ttattctata
    14701 aatcttactc ctaagtatac cacatttgcc cctagatgtg aacgagagaa acgctctaga
    14761 cattgccaag aaggaaaaaa aagggtacaa tgtaacaaaa tcaagggagg tctggaatga
    14821 agaaacaaag caagtacaaa gaggtcgttt cctatctgaa aaatggtatc gagtctgaac
    14881 gatttccgac gggtagtcgc ctgccttcta tccgtcaact gagccttgac tttcactgca
    14941 gcaaggacac cattcaacga gccctgctgg aattacggca cgaacaatac ctctatgcca
    15001 agcctcagag tggctactat gtattagaac aagggcaaca tcaagaccta gaaatcgagg
    15061 ttaccgacga acatgccagt gcctatgacg atttccgact ctgtgtcaat gaaaccttga
    15121 ttggacgaga aaactacctc ttcaactact atgacaatca agaaggatta gaagacctaa
    15181 gacagtccat tcacaaactc ctatttgagc aagctctcta ctgcaaggct aaccaactag
    15241 tactgacttc tggaacccaa caagccttgt ttatcctctc tcaaatatcc tttcctagac
    15301 aagccaagga aatcttggtg gaacagccaa cctaccatcg gatgaatcgc ctcttgattg
    15361 cacaggggct ggactatcaa acgattgaac gaggcattga tgggattgac ttggaggagc
    15421 tggaaggcca cttcaaaaca ggaaaaatta aatttttcta caccattcct agatttcact
    15481 atcctctggg acattcctat tctgagcaag acaaactatc tattcttaac ttagctgcca
    15541 agtatgatgt ctatatcgta gaggacgatt atctgggtga tttggactcc aagaagggcc
    15601 aaaccttcca ctatcttgat acagaggagc atgtcattta tatcaagtcc ttctcgacca
    15661 gcctttttcc tgcccttcgt attacagcac tcattcttcc aaatgctatc aaagaagcat
    15721 ttgtggccta caaaaatatc ctagactacg acagcaacct cattatgcaa aaggccctgt
    15781 cactctatat tgacagtcaa ttgtttgaaa aaaatcgttt ggctcgcttg accaatcatg
    15841 aatcttacca aaaacaaatc gaggaaagga taactaaaac accttgtccc cttcctcatt
    15901 atcccctaca cgatggttta ttgctagacc tgagacagta tcctaaaatc gccagtctca
    15961 aacacagtca actgggcttg gacttctttg aagaggccta tttaagcacc tgtccttatc
    16021 aatttgccaa ggtgtcccta gacaatctgg aaaatgtttt aaactattta aaagcagaat
    16081 tggaatgaca tccaactctg ctttttctta ttcttcaact tcagttagac ttgctgcttt
    16141 ttcaagatag ctttggtaag ttccttcatc cttaagtttt tggatgacct tgtctaccac
    16201 ttctttcaaa tcatcactat tttttctaag agcaacggca ttggcgtcgc cgtccttcat
    16261 cttcaagctg acagttgcga cagctaagcc agcgttttta gcagcataac taagtgcaac
    16321 aggctcatcc atatgaacag catctatttt tccagcctgc aattcattga ctgcttcacc
    16381 catattagtt agggaagtta attgaacttt tggcaattgt tccttgacca ttgattctgg
    16441 aacagtccct ttttgggctg caatattagc actttttagg ttagttaaat ccttgtattt
    16501 ttccacatca gccttacgaa ccaagaaact aatcttgttt tcatagtatg ggattgaaaa
    16561 atcaaagact tcttttctct cgtcagtagc actaattcct gcaactgcta ggtcagcctt
    16621 accagtttga agactggtca aaacattatc aaaactcatg cttgagattt ccaacttaac
    16681 cccaagttca tcagcgatag cctgagccat gtcgatgtct gcaccgacta cctggttctt
    16741 tccatcaacc aatgattgaa attcaaaggg tgcatagtca ggactggttg ccacaactaa
    16801 tttccccttt tgcttaatgg cctcaacagc tgactgagaa ccattagaac ctgactggca
    16861 agctactaag aagaaacttg caagaaaatt acataaaaca aatatccatt tttttgattt
    16921 cataaataaa caacctctct gttaattttg tataattata acgatatcca agttacttgt
    16981 caagtgtttt ttaaattttt atctcaaaaa tattttttcg ttcaaaaaaa ggagccatca
    17041 gttgatttca agctcctttt tatacagaat taaactattt tatagttcga caatcttacc
    17101 tgtttcaaag tagacaaccc attcacagat atttttagca tagtcaccga tacgctccaa
    17161 gtaggaaata acttggaaat aatcacgacc cgtaacaatg gcttctggat ttttcttaat
    17221 ctcttcagtc gcaaggtcac ggatagtttc aaaatagtgg ttaatttgct catccatgga
    17281 ggccacccgg tatgcgtcgt caacagaacc attaagataa agatcaagtg ctgcttcaac
    17341 aacgctttta acttcacgtc ccattttttt aatttcttcc tctacagctg gaatgcgctc
    17401 ttcccccttc atacggatgg ttgcctgagc aatggctaca gcgtgatccc ccatacgctc
    17461 cacatctgat acagccttaa ggacagtcaa gactgtacgc aaatcttgag agactggttg
    17521 ttggagtgcg atcatttcaa atgatttctt ttccagtttc acttcgtatt catttacttc
    17581 tgcatcatct tcgatgacct cttttgccag gtcacggtca tgcgtgacaa aagcacgtac
    17641 cgtacgattg atttgtgaga gcacttcttg tcccatagcg tagaactggt tatgtaattt
    17701 ctctaaatct tcttcaaatt gagatcgtaa catctttcat ctccttatcc aaattttcct
    17761 gtaatatagt cttccgtttc cttgtgttgg ggattaagga acatctgctt ggtatcatta
    17821 aattcaatca aatctccatc taggaaaaat cctgtcttat cagagatacg tgaagcttgc
    17881 tgcatggaac gggttaccag aagcatggtg tacttgtctt ttagaccata caaggtttcc
    17941 tcaattttac cagctgaaat cggatccaaa gccgaagttg gctcatccaa gaggatgatt
    18001 ttaggactag ttgccaagac acgggccacg cagacacgct gctgttgacc acctgacaat
    18061 ccaatagctg aatcatatag acgatccttg acctcatccc agatagaggc accttgcaag
    18121 gctttttcta cggcttcatc cagaacctgc ttatccttaa ttccattgat acgaagcccg
    18181 tagacaacat tctcatagat agtcataggg aaaggattag gttgttggaa aaccattccg
    18241 atttccttac gtaattcaac cgtatctgta cgcggactgt agatgttgtg accattgtac
    18301 accacggatc cagttgtggt cacctctgga ttgagatctc ccatgcggtt gagagacttg
    18361 aggagggttg acttccctga tccagatgga ccaatcaagg ctgtaatttc cttaggttgg
    18421 aaagataggg aaacactatt caaagccttc tttttattat aataaacgga caggtctgat
    18481 acctgtaaaa tcgcatctgt catacggttt cctttctaac caaagtgacc agatacatag
    18541 tcattggtgg actgtagctt ggcattttgg aaaatagttg cagtcttgtc atactcaatc
    18601 aaatcaccca agtaaaagaa gcctgtatag tcacttgcac gagcagcctg ctgcatatta
    18661 tgcgttacaa tgatgatggt aaagtttttc ttgagctcaa acatggtctc ttctagttgc
    18721 atggtcgcaa tcggatccaa ggctgaggct ggctcatcca ttaagaggat atctggctta
    18781 acagagatgg cacgagcgat acagagacgt tgttgctgac cacctgataa ggtcaaggct
    18841 gacttgtgga gatcgtcttt aacctgatcc caaagggcag cctgactaag ggaggtttct
    18901 acgatttcat ctaggacttg cttatcctta actccagcac gttcatgcgc aaaggtaata
    18961 ttacggtaaa ttgacttagc aaatggattg gggcgttgaa aaaccattcc aatgtgttta
    19021 cgcatttcat aaacgttgat ttctggacgg ttgacatcaa ttccacgata gagaatctgc
    19081 ccagttactt tagcaatatc aatggtatca ttcatgcgat tgagactgcg taagtaggta
    19141 gatttccccg atcccgacgg accaatcaaa gctgtaattt tatttctttc aaattgcata
    19201 tcaatcccct taatggattc atttttacca tagtaaacat ggacatcctt agtagaaagg
    19261 gctacttttt cttcaggaaa ggtaaggata tgcttctcat cccagttata tgttgacatg
    19321 gcttctcctt taggcagcgg ttaatttctt gtgtagatag cttccgaact tacgagctcc
    19381 aaagttaaaa atcaggataa agatcaggag cacagcggca gaacctgctg atacaatggt
    19441 tccatctgga atagtgcctt cactattgac tttccagata tggacagcca aggtttctgc
    19501 ttgacggaag atagagatgg ggctagtcac actgaggata ttccagttag accagtcaag
    19561 agctggcgcc gattgccctg ctgtatagat cagagctgca gcttcgccaa agatacgacc
    19621 agatgccaaa acgacacccg ttacaatacc tggaagcgct tctggaataa caacatgaac
    19681 cactgtctcc cagcgagaaa tcccaagagc cagaccagcc tcacgttggg tatggtgaac
    19741 gtgtttcaaa ctatcctcta cattacgcgt catctgaggc aagttaaaga ctgtcaaggc
    19801 caaggcacct gaaatgattg aaaatccata ctcaaactgg actacaaaga tcaagtaacc
    19861 aaagagaccc accaccactg atggtaaaga ggacaagatt tcaatacaag tccgcacaaa
    19921 gttggtaaca ggaccttttt tagcatattc agccaagtaa atcccagctc ccatagaaag
    19981 aggtacagaa ataatcaagg taatgaccaa taggaaaaag gaattgtaaa gctgaatgcc
    20041 aatcccacca cctgcttgaa aagcagaaga ccttccagtc aagaaagacc aagagatatg
    20101 gggcaagccc cgaaccaaga tatagagaat caaggaagcc aagattgtca caatgatgct
    20161 agcaatcgta tagaggacag ctgttgcaag tttatctaat ttcttagcgc gcataatttt
    20221 tctttcctct ttctttcgta atcaatttaa tcacactgtt aaaaactaaa ctcatcaaga
    20281 gcagtaccaa ggccagtgac cagagaacat tattatttac agttcccatg acagtgttcc
    20341 caattcccat agttaatata gaagttaaag ttgcagctgg tgtggtcaag gaagttggga
    20401 taacagctga gtttccgaca accatctgga tagctagagc ctcaccaaag gcacgcgcca
    20461 tcccaaagac cactgcagtg aaaataccag aacgggccgc cttcaagatc acacgccaga
    20521 tagtctgcca gcgagtggct cccatagcga aactggcttc acgataataa cgaggaaccg
    20581 cacgcaagct atccgttgtc ataaaggtta cggtcggcaa aatcatgaca aagaggacgg
    20641 aaatccctga caaaatccca aaaccagtcc caccaaagac actgcgaaca aagggaacga
    20701 cgacttgcaa gccaataaat ccgtacacta ctgaaggaat cccaaccagg agttcaatag
    20761 ctggttgcaa aatcttcgcc ccttttggtg atacttcggt cataaaaact gctgcaccaa
    20821 tagcaaaggg tgttgcgata agggctgaga gaatggtaac gataaaggaa cccaaaatca
    20881 taggaagggc accaaattct ttactagaag gattccaagt tcctcccaaa agaaagtcaa
    20941 agatattcac accattgaca aagaaggtcg acaagccttt ttgcgctacg aaaaccaaaa
    21001 tcatggccac aaggatgact atcaaagaaa gacaggcaaa ggtcaaacct tttcctaatt
    21061 tctccagacg agaattcttt gatggaagca acattttctt agctaattct tcttgattca
    21121 ttattgtctc ccttccaaca ctgtcacagt tccggcagca tctttttcaa ccttcatttc
    21181 cttaatcgga atatacttca atcctttgac aatcccttct tgggtctcat ccgagagaac
    21241 aaaattgaga aattctgcag ccaactcatt gggctgcccc aatgtataca tatgctcata
    21301 agaccacaag ggccaattat tgctacttat attttctgga cttaagtcat agccattcaa
    21361 cttcatgctt ttgaccgaat catctatata ggtaagagat aaataagaga tagctcctgg
    21421 actttttgat acgattgatt ttaccgctcc atttgaatcc tgctcctgac tttgcatggc
    21481 agactgacct tccataatga cagtatcaaa ggtagcacga gagccagagc cggctgcccg
    21541 attgataaca gagatgggta agtccttacc accaacctct ttccaattgg ttacctcacc
    21601 tatgaagatt tgacgaagtt gctctgtcgt taggttatca acatcaacct ccttattgac
    21661 aatcagagcc aagccagcta ccgcgacctt gtggtcaaca agagcagaag catcaattcc
    21721 gtctttttcc tcagcaaata catctgagtt tcctatatca actgccccag actgaacctg
    21781 ggacaagcct gtaccagaac ctcccccttg gacattgacc gtttttccaa catggatcgt
    21841 gccaaattca tctgccgcta cttcaaccaa gggttgcaag gcagttgagc caacagccgt
    21901 tatggattct ccacgatcaa tccagctagc acagcctact aaacaagccg tcagccaaaa
    21961 agcgataaga gacagagcaa gcttttttct ttttttcact gtttttctcc tcgaaaataa
    22021 ttatgaatac tgtgaatttt ttaagtagtt ctttatgagt tgacgcatga attcttacca
    22081 aatttctgcg caattgatta tttatataat ataggctata ttactctttc ctaacctcct
    22141 tttttcatat gtggataaaa tctcttgtct atcccttccc ccattgtcac ccattatagt
    22201 catttcgtgt ctctttttcc cctttttaat gcaagggaaa ttactcttct tagatgataa
    22261 tccaaaagct agaaaggtat ctcaaacctc tctactctcc cagactagtt tacaactaaa
    22321 aggaaaagat tctattttat gagaaatcta gtttacaagc ggtaagaacg ctaataacta
    22381 aacttcttgt actctttgaa aatctcttca aagcagtgtt ttgagctatc tatggctagc
    22441 ttcctagttt gctctttgat tttcatcgag tagtaaaact acatgtgatg gcaatcaaga
    22501 tatcaagaat catcctacta aaaaaatcca tactttcact ataacataga ataagatatt
    22561 tgactagcat tttcatttga atctgaggcc ttttggaaaa taatttttca aaacatttcc
    22621 agtaaccttt gcaaagccca agccattgcc tttaaccaaa acttggtacc aaccatttgg
    22681 cagactttct gccagctgaa cggtttctcc agccgcatac ttgacaaacg cttcttggcc
    22741 aatttcaacc gactgttcga cctgactcgg tttcaaggct aaaccaagag cgaaactggg
    22801 ctcaaagcgt ttcttcttaa aagtacccag atgcagtcca ttgcgagcaa tcttgagctt
    22861 ccctaaatct ggcaaaagtt ctggcaagag ataaagctgg tctccaaaaa tctgcaagat
    22921 acccggtaga ttgaccttca aatggttttg ggcaaattcc tgccacaagg caacttgttc
    22981 acggctgagg ttactcttac ttgccttaaa tttaggagct ggattgttac ccttaaactg
    23041 tagatgggca acaaactgac cctctccctt aaactgatga gggtacatcc gagccgtttc
    23101 ttgcaggtca ataccagcta ccattccatt gatatgctct actggcaaca agtcaaaatc
    23161 atactcttcc agcaaccaat tgacaatctc ttcgttttcc tcgggtgccc aggtacaggt
    23221 cgaataaacc agacgaccac cttcagctaa catggtcact gcatcctcca gaatttctct
    23281 ttgcaagcta gcacattgac tcggataatc taagctccaa tagtccatag catcaggttt
    23341 cttacgaaac attccttcac cagagcaagg ggcatcaaga acgattaagt caaaatagcc
    23401 tttaaagacc ttgaccaagc ggtcggcaga ttcattggtc accacgacat ttgtcgctcc
    23461 aaaacgctcc atgttttcaa ccaaaatctt agcccgtttg cttgaaattt cattggaaac
    23521 aagtagcccc tcccctgcta gataggctgc cagttgagtt gatttgcccc ccggtgcagc
    23581 agccaagtcc aagaccttca taccaggact gggttgggct acttgagcca ccatttgagc
    23641 agcaggttct tgcgaataaa ctaaacctgt agcatgctca ggcgatttcc ctgaaacctt
    23701 cccatagtgg ccccaagggg tttgagtaat ggcatcagaa aaggaaagtt gctcttcttt
    23761 taagggattg acccgaaagg ccgaaaccgc ttcctcctca aaagaggcaa gaaaatctct
    23821 tgcctcttct cctagtattt ctttatattt ttcaacaaat ccttctggaa atttcattta
    23881 agttcttttc ctttcgtaaa tataggactg aatttcctcc tgcatctcaa gaggcaccat
    23941 catgaccggc tgtctggttt gaaaatcagg agcttcacca aaaagggtca caacccgata
    24001 gcccagactt tcccctaaaa tactagctgc ggcataatcc catggttgca gataggtgag
    24061 ataggtcaac aaatgccctg acaaaatctt ggcaaaacta atggccgcac ttccatagac
    24121 acgaacacca agaaccgctc ggctcaaatc agccagcccc cattcattgg tttccagcat
    24181 accactattc cctgcaatga gaaaatctcc aagtggttta gttttaaaag gagctagggg
    24241 cctatcattt agacaaactg gaaattcccc accaccgtgg taacaatccc ctttgaccac
    24301 atcataaatc agaccaaact gtccctgacc attttcaaaa taagccatca taacagcaaa
    24361 atcttcctgc tgggctacaa aattattggt accatcaatg ggatcaatga cccaaacctt
    24421 gccctcttga accgaggctc gcagacaacc ttcttcagca caaatcttat cctcaggata
    24481 acgggacaaa atctcaccaa ccaagagttc ctgaacttct ttgtccagtc tggtcaccaa
    24541 atctgttgga gaggacttgg tttcaacacg caagtcttcc tgcatatggt caagaatgta
    24601 ctggcctgct ttcttaacaa gctctttagc aaattcaaat ttactttcca agagaaatct
    24661 ttccttcccc tttttctttg gcagcctgaa ctgctcggta aagtgaatag ccactaaccg
    24721 tttcaaattc tcgtcccaaa cgtttctctt cgctcttgct tggcacgacc gctttgaatc
    24781 ccttatagga gtccagtaac tttttagcct ctactttgcc ttcataggca gcttcaacat
    24841 cattaaaaaa agaaagcact gaagcaagtt cttcagtgct ccacgacaaa tctagtgggt
    24901 aactatactg tttgttcatt aactaatacc agctctcatt cttgcttctt ttagttcttg
    24961 cttacgataa ctacgaggga gaaaagcacg aatctcatct tcattaaaac cgatttgcat
    25021 acgcttggca tcaataataa ttggacgacg caaaagacta ggatactgct caatcaaatg
    25081 aagcaattcc gataccgaaa tactctctac atcaatattc aatttttgaa aaatttttga
    25141 acgagttgaa atgatgtcat cagtaccatt ttcggtcaag gaaaggatgt gttgcaattc
    25201 ttttcttgtt aaaggactgg tcataatatt gtgttccaca aagggaacct tatgtttttc
    25261 taaccaggcc ttagccttac gacatgatgt acagctcggt gatagaaata gtgtaatcat
    25321 gcttttctct tcttatctat actttgctac ttctattata caaaaaaata aagcgcttga
    25381 ctagggattt ttagaaaaaa agcctatttt ttcaagaaaa ataggctttt tgcgaacgat
    25441 tgacacaatt ggatttggtt aattcactct taacgatggt tttaaacgat atatattttt
    25501 atatatgtaa attaaaaaca tctttccttt cacttcctac gacttttcag atacagatag
    25561 ccaaagaagt tttcatagag ggcaaaaaag aggaggaagg catgaagaaa gaaggtctct
    25621 ggcaaaatca taataacagg atccttggct ggatcaaaaa gccaggtatc atctcccaca
    25681 aagagaattt gatggaaaag agtaaagaat tggtcaaaac caatcaaaac tcccccaagt
    25741 ccaatcatca caggtaagac tactagagcc aggagacttt ttcgataaag agacaaaaag
    25801 tcctttttca caatcctatt gacaaagaca tagaaacttg gcagtgtcac tagagctact
    25861 agctgaacca aatgaaagag attcttgacc actgcgaaat ggtgcagacc agctgctgac
    25921 gaacgaaaat caggcatctg taagacctga ctaaaaggat tggtcagata attcatcaag
    25981 atatgaaaat tgtattgaat ggtttctggt tttagataga ctcgattcgt taagtttagc
    26041 cactgaatct ccataggata gaaaatccaa gccagataaa tggtcagaag gattgagagg
    26101 gagaggagaa agagcataga gccccaaaag gtcaatttag ttttcatcaa aatcccactc
    26161 cgcaaggcta gaaaccacat gtgtcggtgc gattggcagg ccagccactt cttctgcctt
    26221 agtaaaacct gtcgtcacca agagcgttgg aatgccattg tcaatcccag cccggatatc
    26281 agtcaaataa ttgtccccaa ccatgattaa ctcttcacgt tccaaaccta agtgctcaac
    26341 cgccttgtcc ataatgatgg catttggttt tccgatataa accggcttca ctcgtgtcgc
    26401 tacttcaagc agcgtaatca gtgagccagc acctggcaaa agaccacgtt ccgtcgggat
    26461 gttgaggtca ggattggttc cgataaaatg ggcacccttt tgaatagcaa gagttgctgt
    26521 ggcaaatttt tcatagtcga cttgccaatc cagaccaact accacgtagg cagggttttc
    26581 cttgtcttcc acataaccag ccgccttgat ggcttccttg agtcctgctt ctccgacgac
    26641 atagacagtc ttttcaagcc ccaaatcatt catatagtcg atggttgcca aagtcgctgt
    26701 gtagacagtc gatagaggcg tatcgatatt aaaattctga gccaacatct ccttaacact
    26761 ctctggagtg cgggttgtat tgttggttac aaagagatag ggaatgtccc gcttttgcaa
    26821 ttcatgaaca aaagtctctc cagcagggat tcggtctttc cccttataaa tggttccgtc
    26881 taaatcaatt aaatagcctt tatatttcat ctatttctcc ctaaaccttt tttatttctt
    26941 gccaagtaat gattgcttgg gcattgataa ccccatcact tgtaatttca tgcttgcttt
    27001 ccagtccagt ccgttcaaca gccgatgtaa tcaccccacc tggtcgaact tccttgacat
    27061 acttgaggtt gattttcttg ggaatatagt gggtcaaaaa atccgctccc atgacctcaa
    27121 aaatccagtc caagtattta ctgttattga catgaccatt catatccaag tcgtaaaaac
    27181 gaacatggta atccttgctg atcggctctt ccaaggattc atacttcggt ccacggataa
    27241 gttttttatc aaaatcagac tggtaaggag ccacaatctc aggttcaaca gcatggactt
    27301 ttcgactgtc gcggtccatg agaacaaagg tcgccatcat atggatgagc tcctgccccg
    27361 cttcattata aatagtaaag cgacggtagc aaaaaagtcg attgtagctc aaggcttccg
    27421 tttcgatggt aatttcttcc gcaaaacgag gcaaacgaac cacctcaata tcatattctg
    27481 cgataatcca gaccagatta tagtcttcca aaatggcctt atcactaact cccagttcaa
    27541 tcgattgcat ccctgaaact tgcagtgaca gcaaaatcac atctggaagt ttgatatgac
    27601 cgttcatatc agccatatca aaaggaattt tcattttcat ttgataagtt aagcccatga
    27661 tcctactcca aaataaatcg ttctgctaca gtatctccca aaaagagacc tctctttgtc
    27721 atgcgaacgt ggtcaccctc aatctgcatg aggccttgtt gaaccaaatc tctgacaatt
    27781 tctccataaa gtccagcaaa agactgtcca aatttttcct caaatcgcgc catggaaacc
    27841 ccggatttct tgcggagtcc caagaacatt tcttcttcca tttgctcctt ttgactcagg
    27901 tgatcttctg taatacaagc attgccttcc tcaaccgcac tgagataatg acgaatggga
    27961 ccatgatttt tatagcgtac tccattgaca taaccagatg cccctgcacc aataccatag
    28021 tattcagcat tgtcccagta catgagatta tgacgacttt caaagccggg tttggagaaa
    28081 ttagaaatct cataatgctc aaaacccgct cgctccagct ctgcaatgat gtactcaaac
    28141 atctccgctt ctagttcctc cttaggcaga ggcaatttcc cacgtcgcat ccggttcata
    28201 aagaccgtat ggttttctaa aatcaaacta tacaaactca tgtggggaat atccaatcca
    28261 atggctttag ccacattttc ctttacttgc tccatggtct gaccaggcag agcataaatc
    28321 aaatcaatgg agatgttgtc aaaaccagcc agtttcaggc gatcgatatt ttcataaata
    28381 tccttctcca aatgactgcg cccaatcttt ttcaacatct tatcatcaaa ggtctggaca
    28441 cctagcgaaa cacgattgac agccgaattt ttcaaaacag ctatcttatc cgcatccaaa
    28501 tcgcctggat tggcttcaat ggtcaactct tccaagacag acaaatccaa gtttttagtc
    28561 aagccattca gtaacacctc cagttgcgga gccgacaggg ctgtcggtgt tccaccaccg
    28621 atataaaggg ttgacaactt ttcaatatca taagaacgaa actcttccag cagatgctct
    28681 aaatagctgt cgactggctg atttttgatg aagacctttg aaaaatcaca ataataacaa
    28741 atctgggtac aaaatgggat gtgcacatag gctgacgttg gttttttctg catagtaatt
    28801 attataccac aaagactaga ttccagataa aaatcaccat ccccagatac atagtccgtc
    28861 cggagatggt gatggtttat tcttctgtta tatcaatcac aatctcttct gagtcatcaa
    28921 gagcttcggc tttttcttgc cattgttcct tgagattatt taattgattt tttgatgctt
    28981 ctgtcgcttg aaaagcatag gatttagctt gagcaagtat actgtccaca gtgatttcac
    29041 ctgactcaac ctgttctttt gttttcagaa caaaatctgt agcctgctcc ttaacttctg
    29101 tcagtttttc acagacttgc tccttggcat actccggatc ttctctcaaa tcatctagaa
    29161 aatcttgagc ctgactgcaa acttgtttgc ccttatcact tgttaaaaac aaggcaagag
    29221 ctgcacctga aacggttcct aaaaggattg aggataattt acccataagg attctccttt
    29281 tttatttttt gaaaaattta cttgcaagac gaagagctga cagacttgca ccagtcttga
    29341 gtgtttttga accagctgat gaagctttct tgctcaagac acgcgcatgg tcattgaggt
    29401 ctgaaacaga tagagataaa tctgcaacag cactgaagag tggatcaatc gtagccacct
    29461 tgacattgat atcatctgcc aagacattga ccttagccaa caactcattg gtgtgatgca
    29521 aggtcacatc cacatctgaa gtcaaggttt taatcgtctt ttctgtttca tcgatgacac
    29581 gaccaagctt ttgtacagta atgatcagat agaccaaaaa gacaatcaca gctagggcaa
    29641 caagaatata tgcaacttct aacatttagt tttcctcctc tgtaatatag taaggggcct
    29701 tctttcgatt ttgataaata acgatcatta taccgagacc gataaagaca actgacagcc
    29761 attgggacac tcgaaggccg aagaacatga gactatctgt tcgcatacct tcgatgacca
    29821 tacgaccgaa accataccaa atcaagtaaa aggccgtgat atgacctcgt ctgagactct
    29881 tccatttccg tctaaaaatc agaatcaagg caaagccaag cagattccat agagactcat
    29941 aaaggaaagt cggttgacgg tagctcccct caatatacat ctggtcacgg ataaagccag
    30001 gtagataatc cagattatcc actgttgcac cataagcttc ttggttaaag aaattacccc
    30061 aacgccccaa actttgagca atcataacgc taggcgccgc aatatctaga aaatcccaag
    30121 tattgatgag tttacggtca gcaaagatat agagcacaag agccccagtt atcaaaccac
    30181 cgtaaatggc caaaccacca ttccaaatgg caaaaatctc tcctaaattc tgactatagt
    30241 aatcaaatcg gaaaataaca tagtagagac gagctcctaa aatagccaag ggaaaggcta
    30301 ctaagataaa atctaaaata tcgtctggta tgatcttctt tctaggtgct tctttcatgg
    30361 tcaaataaac cgcaagaatc aagcctgtca caatacataa ggcataccaa cgaatggcta
    30421 ggggtcctag ttgaatagca attggatcaa gcattttgca cctcatttcg agctatgaga
    30481 cttgtcagtc gttcgtcgaa caaacgggta gcatcaaagc ccatttcctt ggcacgataa
    30541 ttcatggcag ctgcctcaat cacaacagag atattacgac ctgttttaac tggaatacga
    30601 atacgaggaa tggctacgcc agaaacttca agttcctctg cattgtttcc aagacgatca
    30661 aaggtcttat gcgtatcgta attttccaaa tagacagcaa gctgaacctg tgaagaatcc
    30721 ttgacagcac tcgcaccgta gagactcata acatcgataa taccaacccc acgaatttca
    30781 atcaagtgtt tcaaaatttc agctggttca ccccagagag taatctcatc cttggcaaag
    30841 atatcgacac ggtcatcggc taccaaacgg tgaccacgtt tgacaagctc aagacctgtc
    30901 tcgctcttac caattccact atctccctga atcaagacgc ccatcccata aatatccatc
    30961 aagacaccgt gcacactggt acgttctgcc aaacgagaat ccagatagct agataactct
    31021 ccagataaac gactggtagc tgcacggctg gttaaaatag caatcttaca ttctctagca
    31081 gcctttaaca tctcctctgg aaccaccaaa ccacgggcaa caatgactgc tggtgtctca
    31141 ggtagaaaca tttttttcaa aacttcataa cggctgttgg aaggcatgct gatcagataa
    31201 gaccactcct tcatccccaa aagttgaatc cgctctggtg tatagtagtc aaaatagcct
    31261 gtcatttcaa gaccaggtcg cgtaatatcc gctgtattga tttccttttc aagcaattct
    31321 ggttcaccat agacaatatc tagtctaagc ttttcaatca cttcttttac taaaaccgac
    31381 atcatttcct ccttcaaatg aattcttctc tctattatat caaattgtag gaggaagttt
    31441 cagatttttg caggctttgg agtatttatt taaaagaaaa aggactagat ttctctagcc
    31501 tttcatttca attagaattt tttacgttta cgagctactt ctgatttacg tttacgtttt
    31561 acagaaggtt tttcatagaa ttcacgtttg cgtgtttctt gaagagtacc agctttagta
    31621 accgcacgtt tgaaacggcg aagtgcgtcg tcaagagatt cattcttacg tactactgtt
    31681 ttagacattt ttttcactcc cttcaagttc aatatctatt tcattctaca cgtaaaagga
    31741 ataattgtca agcaaaaatg cggtttaatt ttgatttttt cttccattta tacaagagtt
    31801 gaaagcgttc aatagaacat tgctttttta tttttcgagt aagctaagcg cttcagcatc
    31861 tgcgatgatg gttacatcag ggtgattttg gaggctactt gcaggtaggt tctcagtcac
    31921 tgggccagat actgttccgg caatggcttc tgctttcgac tcaccataag caaaaagaat
    31981 aatagacttg gcatccaaaa tgtttttaat ccccattgaa atagcttggg ttgggacgtc
    32041 ttcaatcttg gcaaagaagc gagcattggc ttcgatagta gactggtcaa gttctactag
    32101 atgcgtttga ctgtcaaatg gagtaccagg ctcattaaag ccgatatgcc cattgcgacc
    32161 gattcccaag atttggaggt caactggatg gtcagccaaa atttggttgt agcgttcaac
    32221 ttcagcttca gcattgtcct taacaccacg aggcaagaaa ctttctttaa atggtttttg
    32281 gttgaacaag ttttcttgca tgaagtaacg ataagactgt ggattgtccc catcaaggcc
    32341 cacatactca tcaaggttga cactggttag atttgaaaaa tcaaggtcac tctcaacaat
    32401 ttccttgtaa aattcaagtg ggctacttcc tgtcgcaagt cctagagttt gagctccatt
    32461 agccaatttt tccttcaaaa tctcaaaagc aacttttcca ccttgaactt ggttttcaac
    32521 tttaataact ttcatctttt catcctccat ttctgtctat atttattata tggtatagac
    32581 caatttttgt caagtgaaaa cgatttaatt tctaaaaaaa gagcgtccaa acttgaaacg
    32641 tgttataatg gataggatta gaacttagaa agaatgaaca gatatatgaa tacagctgat
    32701 tttgatttcc acttgccaga ggaattgatt gcccaaacgc cccttgaaaa acgtgatgcc
    32761 tccaaactcc tcatcgtcaa ccgtgagaca ggagaaatgc aagataaaca cttccactct
    32821 attattaata tgctggaacc tggtgatgcc cttgtcatga acgacacccg agttctccct
    32881 gcccgcctct atggtcaaaa agtggagaca ggaggtcatg tggaacttct cctccttaag
    32941 aacactagtg gagacgagtg ggaagttctg gctaaacctg ccaaacgcct caaggtcggt
    33001 actcgtatca gctttggtga tggccgcctc agcgctgtcg ttacagaaga attgacccac
    33061 gggggacgca ttgtccgctt tgaataccaa ggaattttcc tagaagtctt ggaaagtctg
    33121 ggagaaatgc ctctgccacc ttatatccac gaaaaattag atgaccgtga acgttatcaa
    33181 accgtctacg ccaaggaaag cggctctgct gcagcaccga ctgctggtct tcacttcacc
    33241 aaagaactgc tggcagaaat ccaagctaag ggtgttcatc tagtctatct gactctccat
    33301 gtcggactcg gaacctttag acctgtttct gtggataatc tggacgaaca cgaaatgcac
    33361 tcagagttct atcaactttc tgaggaagct gctgccaccc ttcgctctgt caaaaaaaat
    33421 ggtggtcgtg tcatcgctgt cggaaccact tctatccgca ccttggaaac tattggttcc
    33481 aagtttgatg ggcaaatcca agcagattct ggttggacca atatctttat caaacctggg
    33541 tatgagtgga aggtcgtgga tgccttctca accaacttcc acctgccaaa atcaactctg
    33601 gtcatgttgg tttctgcctt tgcaggccgt gaattagtct tagatgccta ccaccattcc
    33661 atccaagaac actaccgctt cttcagtttt ggtgacgcta tgtttattta ttgagaaaga
    33721 atttctctaa atcttctaat accaataaat cgctaagata ttatttcaaa gaacatctac
    33781 aattgaaact ctagctagct gtagaagagg cctagtacat tgaaattaaa atgcttcccc
    33841 ctagcttcga aaatattgcc atagattgcg ttgactctcc aaattgattc atctatattt
    33901 tatttcagct tcctatactt tcttcgctgt ttgtaaatca aaatgcaaga cacatgagta
    33961 gcaccatatt tgttactctt atctgtcctc tcaagagact attatgagtt atttcagaat
    34021 cattcactac tttgaccctg actctcctta gtctcaaaat caaagactta tactcttcaa
    34081 aaatctcttc aaaccgcgtc aacgtcacct tggattatat atgtgactga ctttgtcagt
    34141 tctatctaca acctcaaagc agtactttga gcaacctgcg actagttttc tagtttgctc
    34201 tttgattttc attgagtatt aaacaaaaag tgaacaaatc tgaattctaa tgtacagaag
    34261 actaggcttg ttcacttttt tatagtcgct ataagatgac cttatctata gctttttata
    34321 tataattata tattcagaca tactattatc aattttgtcg cagggaggaa tctgttaacg
    34381 cacccattca ccattatcat tgactctata gccatctata cttgtattga ccgctaactc
    34441 acccgatgta tttacataat accatttacc accaacttgg aaccattgat tgactttcat
    34501 agaaccgttg ctgttgaggt agtaccatga accattaact tgtacccaac ctgttgccat
    34561 ggaaccatca gtattataaa aataccacat accattttct tgtttccagc ctgttgttgg
    34621 agcaactgct ttagctggtt ctactgctac atctgttcct tggttagatg taacagatac
    34681 aggatacgaa ggaatagatg attgctcagg aacaacaact ttttcaggtt ctctcgtccc
    34741 tctccttata cgtcttttta ccatctcttt agtaatttga cgagaagtag tttcttcaat
    34801 tgttccatca cgttcatcta cagcatagat tgtagtaaga gtaattttac caatttctcc
    34861 tacttcttga cctttatcaa gagttgggtc atcgagatat tctgtttcga ttggaatttc
    34921 ttggacaata acttggggct tggttccttt tttaacaact cttgtttgag agtcttcttt
    34981 ttgacttaaa gtactctcag ttacttgtcc actctttcca tctacatcat aagttatcgt
    35041 tgtaactgtt ttcccattct ttcctagagt aatctcttgc tcctgtcctg cagaaaggtc
    35101 attgtctgct tcatatttag tagcacatgg aacaagaact tcttcaacct tgcttttagc
    35161 tggaactttg ataactgtat ccgtggcttc ttttctatca acagtaacct tttcggtaac
    35221 atgaccagtc tctggattaa catcgtaggt ccttgtcgtg gttacatagc catcctctcc
    35281 gtcaattgta acaggatttt cactacggta ttttgtttca tctttttcaa aacgaattcg
    35341 cgtacttgaa attttcttgg ttactacctt aggtttagtc gctactttta caataatatc
    35401 cccattgtca gcgtcatcat actctattcc ctcttcttta tctctagtat catctctgac
    35461 atattgaatc ccatcagcag catgagcaaa acttgtattc agattcctcc taaaaataaa
    35521 gttagcccga ttaccgcaga accaaaaatc tttccgagtt tacgtattgc atagcgctta
    35581 ttagtattag attttgccat tacatcctac ttctagtata gcatcttttc tatcaaacgt
    35641 taaacaatat actttatata taaaatagac tt
//
LOCUS       DAVKAO010000014        34452 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKAO010000014 DAVKAO010000000
VERSION     DAVKAO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.014"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            597..1049
                     /locus_tag="VWX16_000723"
     CDS             597..1049
                     /locus_tag="VWX16_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9158111.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1068..2405
                     /locus_tag="VWX16_000724"
     CDS             1068..2405
                     /locus_tag="VWX16_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9158112.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2484..3368
                     /locus_tag="VWX16_000725"
     CDS             2484..3368
                     /locus_tag="VWX16_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9158113.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3368..4201
                     /locus_tag="VWX16_000726"
     CDS             3368..4201
                     /locus_tag="VWX16_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9158114.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4219..6312
                     /gene="nanB"
                     /locus_tag="VWX16_000727"
     CDS             4219..6312
                     /gene="nanB"
                     /locus_tag="VWX16_000727"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9158115.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIGEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6325..7428
                     /locus_tag="VWX16_000728"
     CDS             6325..7428
                     /locus_tag="VWX16_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9158116.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7602..8300
                     /locus_tag="VWX16_000729"
     CDS             7602..8300
                     /locus_tag="VWX16_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9158117.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8319..9836
                     /locus_tag="VWX16_000730"
     CDS             8319..9836
                     /locus_tag="VWX16_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9158118.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9961..11289
                     /locus_tag="VWX16_000731"
     CDS             9961..11289
                     /locus_tag="VWX16_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9158119.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11541..12407
                     /locus_tag="VWX16_000732"
     CDS             11541..12407
                     /locus_tag="VWX16_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9158120.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12423..13262
                     /locus_tag="VWX16_000733"
     CDS             12423..13262
                     /locus_tag="VWX16_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9158121.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13535..13987
                     /locus_tag="VWX16_000734"
     CDS             13535..13987
                     /locus_tag="VWX16_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9158122.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            14084..14428
                     /locus_tag="VWX16_000735"
     CDS             14084..14428
                     /locus_tag="VWX16_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158123.1"
                     /translation="MVSSKHAARASFLDGQGITVDEMSWIIRGIVTGLIGGHIKLGAY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGRILQIAVNVADVYGNFAYNIAAAWDA
                     HDKIPNNGRINF"
     gene            14713..15357
                     /locus_tag="VWX16_000736"
     CDS             14713..15357
                     /locus_tag="VWX16_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9158124.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRYFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15526..16443
                     /locus_tag="VWX16_000737"
     CDS             15526..16443
                     /locus_tag="VWX16_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9158125.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16461..17345
                     /locus_tag="VWX16_000738"
     CDS             16461..17345
                     /locus_tag="VWX16_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9158126.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASMTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17361..18212)
                     /locus_tag="VWX16_000739"
     CDS             complement(17361..18212)
                     /locus_tag="VWX16_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9158127.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18412..20469
                     /gene="pbp2b"
                     /locus_tag="VWX16_000740"
     CDS             18412..20469
                     /gene="pbp2b"
                     /locus_tag="VWX16_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9158128.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVKALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPYDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20480..21076
                     /gene="recR"
                     /locus_tag="VWX16_000741"
     CDS             20480..21076
                     /gene="recR"
                     /locus_tag="VWX16_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9158129.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21252..22295
                     /locus_tag="VWX16_000742"
     CDS             21252..22295
                     /locus_tag="VWX16_000742"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9158130.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISRSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22379..23752
                     /locus_tag="VWX16_000743"
     CDS             22379..23752
                     /locus_tag="VWX16_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9158131.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLVP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            23739..24350
                     /locus_tag="VWX16_000744"
     CDS             23739..24350
                     /locus_tag="VWX16_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9158132.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQMEQYLD"
     gene            24441..25145
                     /locus_tag="VWX16_000745"
     CDS             24441..25145
                     /locus_tag="VWX16_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9158133.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTIYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGIFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25364..26737
                     /gene="ftsA"
                     /locus_tag="VWX16_000746"
     CDS             25364..26737
                     /gene="ftsA"
                     /locus_tag="VWX16_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9158134.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRTGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26754..28013
                     /gene="ftsZ"
                     /locus_tag="VWX16_000747"
     CDS             26754..28013
                     /gene="ftsZ"
                     /locus_tag="VWX16_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9158135.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28018..28689
                     /locus_tag="VWX16_000748"
     CDS             28018..28689
                     /locus_tag="VWX16_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9158136.1"
                     /translation="MNVKENTELVFREVAEASLSANRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELMEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28699..29238
                     /locus_tag="VWX16_000749"
     CDS             28699..29238
                     /locus_tag="VWX16_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9158137.1"
                     /translation="MSLKDRFDRFIDYFTENEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29238..29501
                     /locus_tag="VWX16_000750"
     CDS             29238..29501
                     /locus_tag="VWX16_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9158138.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSIWVAIVLVRFLGENLVRFLAMIG"
     gene            29498..30283
                     /locus_tag="VWX16_000751"
     CDS             29498..30283
                     /locus_tag="VWX16_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9158139.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30292..31026
                     /gene="divIVA"
                     /locus_tag="VWX16_000752"
     CDS             30292..31026
                     /gene="divIVA"
                     /locus_tag="VWX16_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9158140.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVHDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DLLRPTATYLQTSDEAFKEVVSKVLGEPIPAPIEEEPIDMTRQFSQAEMAELQARIEV
                     ADKELAEFEAQIKQEVEAPTCSESSG"
     gene            31426..34218
                     /locus_tag="VWX16_000753"
     CDS             31426..34218
                     /locus_tag="VWX16_000753"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9158141.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10618 a         6089 c         7489 g        10256 t
ORIGIN      
        1 tactaaaaaa aaaaagtata ctctaattga aaacggtaac aaaactaatt tagagaatga
       61 aatatagagt atttctctct taaaagtttt tggtgaaacg agatgtagaa aggagattta
      121 gccaaagagt ctattagtgc tagaataata gattagaatt attttagaaa aacgaagtga
      181 gcagcttata aattcaagtc cccaaataga ttcatactag tatcttttgc aaaaaataaa
      241 gggcgacttc cttcatgaat atcaatttca tctataagga aggtagctaa ttgaactaac
      301 ttatttattc tgtttgtcgc tagaaaaatc agacctcctt gtgaagattg aggagatact
      361 gaatgaaaat caaagaagaa actagcaagc tagtagcaga ttgcccaaaa caccgctttg
      421 aggttgtaga taagactgac ctatataatc caaggtgaag cgactgtggt ttgaagagat
      481 tttcaaagag tataggctag agagtagtgt ttttatgtcc ttctagtaga aaatgctaga
      541 cagaagaatg gggaacttgg ataggaaaaa tagattgaga aaggaggtta gaagagatga
      601 ttattacaaa aattagccgt ttaggaactt atgtgggagt aaatccacat tttgcaacat
      661 taatagattt tctagaaaaa acaggactag aaaatttaac agaaggttcg attgctatcg
      721 atggtaatcg attgtttggg aattgcttta cttatctagc agatggtcaa gcaggggctt
      781 tctttgaaac acaccaaaaa tatttggata ttcatttagt tttggaaaac gaagaagcca
      841 tggctgttac atcgccggaa aatgtaagcg ttacccaaga atatgatgaa gagaaagata
      901 ttgaattata cacagggaaa gtggaacagt tggttcattt gagagctggc gaatgcctca
      961 tcacttttcc agaagattta catcaaccca aggttcgtat aaatgatgaa cctgtgaaaa
     1021 aagttgtctt taaagttgcg atttcttaat gtagaaagag aagaacgatg aaaaaaatga
     1081 gaaagttttt atgtctagct ggaattgcgc tagcggctgt tgccttggta gcttgttcag
     1141 gaaaaaaaga agctacaact agtactgaac caccaacaga attatctggt gagattacaa
     1201 tgtggcactc ctttactcaa ggaccccgtt tagaaagtat tcaaaaatca gcagatgctt
     1261 tcatgcaaaa gcatccaaaa acgaaaatca agattgaaac attttcttgg aatgacttct
     1321 atactaaatg gactacaggt ttagcaaatg gaaatgtgcc agatatcagt acagctcttc
     1381 ctaaccaagt aatggaaatg gtcaactcag atgctttggt tccgctaaat gattctatca
     1441 agcgtattgg acaagataaa tttaacgaaa ctgccttaaa tgaagcaaaa atcggagatg
     1501 attactactc tgttcctctt tattcacatg cacaagtcat gtgggttaga acagatttgt
     1561 taaaagaaca taatattgag gttcctaaaa cttgggatca actctatgaa gcttctaaaa
     1621 aattgaaaga agctggagtt tatggcttgt ctgttccgtt tggaacaaat gacttaatgg
     1681 caacacgttt cttgaacttc tacgtacgta gtggtggagg aagcctctta acaaaagatc
     1741 ttaaagcaga cttgacaagc caacttgctc aagatggtat taaatactgg gttaaattgt
     1801 ataaagaaat ctcacctcaa gattctttga actttaatgt ccttcaacaa gctaccttgt
     1861 tctatcaagg aaaaacagca tttgacttta actctggctt ccatatcgga ggaattaatg
     1921 ccaacagtcc tcaattgatt gattcgattg atgcttatcc tattccaaaa atcaaagagt
     1981 ctgataaaga ccaaggaatt gaaacctcaa acattccaat ggttgtttgg aaaaattcaa
     2041 aacatccaga agttgctaaa gcattcttag aagcacttta taatgaagaa gactacgtta
     2101 aattccttga ttcaactcca gtaggtatgt tgccaactat taaggggatt agcgattctg
     2161 cagcctataa agaaaatgaa actcgtaaga agtttaaaca tgctgaagaa gtaattactg
     2221 aagctgttaa aaaaggtact gctattggtt atgaaaatgg accaagtgta caagctggta
     2281 tgttgactaa ccaacacatt attgaacaaa tgttccaaga tatcattaca aatggaacag
     2341 atcctatgaa agcagcaaaa gaagcagaaa aacaattaaa cgatttattt gaggctgttc
     2401 agtagatgta aaagactaga aaataggtgg gctagtgagc tgaaaagctc tagcccaatc
     2461 ttgtaaaaga agggagaagg agaatggtta aagaacgtaa tttaactcgc tggatatttg
     2521 ttttgccagc tatgattatc gtaggattac tctttgttta tccgtttttc tcgagtattt
     2581 tttatagctt taccaataag catttgatta tgcctaatta taaatttgtt ggtttggcta
     2641 actataaagc tgtgctatca gatcccaact tctttaatgc gttctttaat tcaattaagt
     2701 ggaccgtttt ctcattagtt ggtcaagttt tagtagggtt tgtattggct ttagctcttc
     2761 acagagtacg ccacttcaag aaattatata ggaccttatt gattgttcct tgggcatttc
     2821 ctacaatcgt tattgccttc tcttggcagt ggattctaaa cggggtttat ggctacttac
     2881 ctaatctaat cgtaaaatta ggtttaatgg aacatacacc tgcatttttg acagatagta
     2941 catgggcatt cctatgtttg gtgtttatca acatttggtt tggagcacca atgattatgg
     3001 ttaatgtgct ttcagctttg caaacagtac cagaagaaca atttgaggct gctaagatag
     3061 atggtgcttc aagttggcag gtgttcaagt ttatcgtctt tccacatatt aaagtggttg
     3121 taggacttct agttgttttg agaactgtat ggatctttaa taactttgac attatctacc
     3181 tcattactgg tggtggacca gccaatgcta caacgacgct tccaattttt gcttacaacc
     3241 tgggttgggg aactaaattg ttgggtcgtg cttcagcagt tacagtattg ctctttatct
     3301 tcttggtggc gatttgcttt atctactttg ctatcatcag taagtgggaa aaggagggta
     3361 gaaaataatg aagaagaaat ccagtattta tttagatatt ctctcacatg tacttttagt
     3421 tggtgcgacc atcgttgcaa ttttcccatt ggtatggatt atcatatctt ctgtcaaagg
     3481 gaaaggggaa ttaactcagt atccaacacg attttggcct gaacagttta cattagatta
     3541 tttcactcat gttatcaacg atttgcactt cattgataac attcgaaaca gtttaatcat
     3601 tgccttggct acaaccctca ttgcgattat tatttctgct atggcagcct atggtattgt
     3661 tcgattcttt cctaaattgg gagcaatcat gtcgagacta ctcgtcatta cctacatttt
     3721 cccaccaatt ttgttagcaa ttccctattc aattgccatt gctaaagttg ggttaacaaa
     3781 tagtttattt ggcttgatga tggtttatct atcttttagt gttccatatg cagtttggct
     3841 cttagttgga tttttccaaa cagttccaat tggaattgaa gaagcggcta gaattgatgg
     3901 tgcaaataaa tttgttacgt tttataaggt tgtgctaccg attgtagcac caggtattgt
     3961 agcaacagct atttatacat ttatcaatgc ttggaatgaa ttcctgtatg ccttgatttt
     4021 gattaacaat acaggaaaga tgacagtagc agtagccctt cgttcactta atggttcaga
     4081 aatactagac tggggagata tgatggcagc gtctgttatt gtagttcttc catcaattat
     4141 tttcttctct atcatccaaa ataagattgc aagtggatta tcagaaggat ctgtgaagta
     4201 gacgaaagaa ggaaaaaaat gaataaaaga ggtctttatt caaaactagg aatttctgtt
     4261 gtaggcatta gtcttttaat gggagtcccc actttgattc atgcgaatga attaaactat
     4321 ggtcaactgt ccatatctcc tatttttcaa ggaggttcat atcaactgaa caataagagt
     4381 atagatatca gctctttgtt attagataaa ttgtctggag agagtcagac agtagtaatg
     4441 aaatttaaag cagataaacc aaactctctt caagctttgt ttggcctatc taatagtaaa
     4501 gcaggcttta aaaataatta cttttcaatt ttcatgagag attctggtga gataggtgta
     4561 gaaataagag acgcccaaaa gggaataaat tatttatttt ctagaccagc ttcattatgg
     4621 ggaaagcata aaggacaggc agttgaaaat acactagtat ttgtatctga ttctaaagat
     4681 aaaacataca caatgtatgt taatggaata gaagtgttct ctgaaacagt tgatacattt
     4741 ttgccaattt caaatataaa tggtatagat aaggcaacac taggagctgt taatcgtgaa
     4801 ggtaaggaac attacctcgc aaaaggaagt attggtgaaa tcagtctatt taacaaagca
     4861 attagtgatc aggaagtttc aaatattccc ttgtcaaatc catttcagtt aattttccaa
     4921 tcaggagatt ctactcaagc taactatttt agaataccga cactatatac attaagtagt
     4981 ggaagagttc tatcaagtat tgatgcacgt tatggtggga ctcatgattc taaaagtaag
     5041 attaatattg ccacttctta tagtgatgat aatgggaaaa cgtggagtga gccaattttt
     5101 gctatgaagt ttaatgacta tgaggagcag ttagtttact ggccacgaga taataaatta
     5161 aagaatagtc aaattagtgg aagtgcttca ttcatagatt catccattgt tgaagataaa
     5221 aaatctggga aaacgatatt actagctgat gttatgcctg cgggtattgg aaataataat
     5281 gcaaataaag ccgactcagg ttttaaagaa ataaatggtc attattattt aaaactaaag
     5341 aagaatggag ataacgattt ccgttataca gttagagaaa atggtgtcgt ttatgatgaa
     5401 acaactaata aacctacaaa ttatactata aatgataagt atgaagtttt ggagggagga
     5461 aagtctttaa cagtcgaaca atattcggtt gattttgata gtggctcttt aagagaaagg
     5521 cataatggaa aacaggttcc tatgaatgtt ttctacaaag attcgttatt taaagtgact
     5581 cctactaatt atatagcaat gacaactagt cagaatagag gagagagttg ggaacaattt
     5641 aagttgttgc ctccgttctt aggagaaaaa cataatggaa cttacttgtg tcctggacaa
     5701 ggtttagcat taaaatcaag taacagattg atttttgcaa catatactag tggagaacta
     5761 acctatctca tttctgatga tagtggtcaa acatggaaga aatcctcagc ttcaattccg
     5821 tttaaaaatg caacagcaga agcacaaatg gttgaactga gagatggtgt gattagaaca
     5881 ttctttagaa ccactacagg taagatagct tatatgacta gtagagattc tggagaaaca
     5941 tggtcggaag tttcgtatat tgatggaatc caacaaactt catatggcac acaagtatct
     6001 gcaattaaat actctcaatt aattgatgga aaagaagcag tcattttgag tacaccaaat
     6061 tctagaagtg gccgtaaggg aggtcaatta gttgtcggtt tagtcaataa agaagatgat
     6121 agtattgatt ggaaatacca ctatgatatt gatttgcctt cgtatggtta tgcctattct
     6181 gcgattacag aattgccaaa tcatcacata ggtgtactgt ttgaaaaata tgattcgtgg
     6241 tcgagaaatg aattgcattt aagcaatgta gttcagtata tagatttgga aattaatgat
     6301 ttaacaaaat aaaggagaaa aaacatggtt aaatacggtg ttgttggagc agggtatttt
     6361 ggagctgaat tggctcgcta tatgcaaaag aatgatggag cagagattac tcttctctat
     6421 gatccagata atgcagaggc gattgcagaa gaattgggag caaaagtagc aagttcctta
     6481 gatgagttgg tttctagcga tgaagtagat tgtgttatcg tcgcaactcc aaataatctt
     6541 cataaggaac cggttattaa ggctgcacag catggtaaaa atgttttctg tgaaaaacca
     6601 attgcgcttt cttatcaaga ttgtcgcgag atggtagatg cgtgtaaaga aaacaatgta
     6661 acctttatgg caggacatat tatgaatttc tttaatggtg ttcatcatgc aaaagaactc
     6721 attaatcaag gagttatcgg agacgttcta tattgtcata cagctcgtaa tggttgggaa
     6781 gaacaacaac cgtcagtatc atggaaaaaa attcgtgaaa aatcaggtgg tcacttgtat
     6841 caccacatcc atgaattgga ttgcgttcaa ttccttatgg ggggcatgcc tgaaactgta
     6901 accatgacag gtggaaatgt ggcccatgaa ggtgaacatt tcggtgatga agatgatatg
     6961 atttttgtca atatggaatt ttctaataag cgttttgcct tgttagaatg gggttcagct
     7021 tatcgttggg gtgaacatta tgtcttaatc caaggaagca aaggtgccat ccgcttagac
     7081 ttattcaact gtaaaggaac tcttaagcta gatgggcaag aaagctattt cttgattcac
     7141 gaatcgcaag aagaagatga tgatcggact cgtatctatc atagtacaga gatggatgga
     7201 gcaattgctt atggtaaacc aggtaaacgt actccattat ggctatcatc tgtcattgat
     7261 aaagaaatgc gctatctgca tgagattatg gaaggagctc cagtatcaga agaatttgca
     7321 aaacttttga caggtgaagc tgccctagaa gcaattgcta ctgcagatgc ttgtacccag
     7381 tctatgtttg aagatcgcaa agtaaaattg tcagaaattg taaaataaat tttggtattc
     7441 tcctatttat aggtcgactt gctcctctga aagtactttt agaggagctg tttgactttg
     7501 ctagtttttg aaactgaaat ctattatact acaaactatt gaaagcgttt taattttaag
     7561 gtataataat ctcatagaaa taaagaaaag gaggaaagag gatgccacag attagcaaag
     7621 aagccttgat tgagcaaatc aaagatggaa tcatcgtttc ttgtcaggct cttcctcatg
     7681 aaccgcttta tacagaagcg ggaggggtga ttcccttgct ggtcaaagcg gctgagcaag
     7741 gtggagcagt cggtatccga gcaaacagtg ttcgcgatat caaggaaatt aaggaagtca
     7801 ctaaacttcc aatcattggg attatcaaac gtgattatcc acctcaggaa cccttcatca
     7861 cggctactat gaaagaagtt gatgaattgg cagaactgga catcgaggtg attgctctgg
     7921 attgtaccaa gcgtgaacgc tacgatggtt tggaaattca agagttcatt cgtcaggtta
     7981 aggagaaata tcctaatcag cttttgatgg ctgatactag tatcttcgaa gaagggctag
     8041 cagctgtaga agcaggaatt gactttgtcg gaacaacctt atcaggctac acatcctaca
     8101 gtccaaaagt agacggtcca gattttgaat tgattaagaa actctgtgat gctggtgtag
     8161 atgtcattgc agaaggaaaa attcatacac cagaacaagc caaacaaatc cttgaatatg
     8221 gagtgcgagg catcgttgtt ggtggcgcca ttactagacc aaaagagatt acagaacgct
     8281 tcgttgctag tcttaaataa gatgtgaggg ggagttttat gtttaaagtt ttacaaaaag
     8341 ttggaaaagc ttttatgtta cctatagcta tacttcctgc agcaggtcta cttttgggga
     8401 ttggtggtgc actttcaaac ccaaccacga tagcaactta tccaatacta gacaatagta
     8461 tttttcaatc gatattccaa gtaatgagct ctgcaggaga ggttgtattc agtaatttgt
     8521 cactacttct ctgtgtggga ttatgtattg gcttagcgaa acgagataaa ggaaccgctg
     8581 cgttagcagg agtaactggt tacttagtta tgactgcaac gatcaaagct ttggtaaaac
     8641 tttttatggc agaaggatct gcaattgata ctggagttat tggagcatta gctgtcggaa
     8701 tagttgccgt atatttgcac aaccgatata acaatattca attaccttcc gctttaggat
     8761 tctttggagg ttcacgcttc gttcctattg ttacatcgtt ctcttctatc ttgattggct
     8821 ttgtcttctt tgttatttgg ccacctttcc aacaacttct tgtttctaca ggtggatata
     8881 tttctcaggc gggtccaatt ggaacttttc tatatggatt tttaatgaga ctttctggag
     8941 cagtaggctt acatcatata atttacccta tgttttggta tactgaactt ggtggtgttg
     9001 aaactattgc aggacaaaca gtggttggag ctcaaaaaat attttttgct caattagccg
     9061 atccggccca ttctggatta tttacagaag gaacaaggtt ttttgcaggt cgtttctcaa
     9121 caatgatgtt cggtttaccg gctgcctgtt tagcgatgta ccatagtgtt cctaaaaatc
     9181 gtcgtaaaaa atacgcgggt ttgttttttg gagttgcttt aacatctttt attaccggta
     9241 ttacagaacc aattgaattt atgtttctat tcgtcagtcc ggttctatat gttgttcacg
     9301 cattccttga tggtgttagc ttctttattg cagacgtctt aaatatttca ataggaaaca
     9361 cattttcagg aggtgtaatc gatttcactt tatttggaat tttgcagggg aacgctaaga
     9421 cgaattgggt tcttcagatt ccaattggac ttatttggag tgttttgtat tatattattt
     9481 ttagatggtt cattactcaa ttcaacgttc taacgccagg acgaggagaa gaagtagatt
     9541 ctaaagaaat ttctgaatcc gcagattcaa cttcaaatac tgcagattat ttaaaacagg
     9601 atagcctaca aattatcaga gccttgggtg gatcaaataa tatagaagat gtagatgctt
     9661 gtgtgacacg tttacgtgta gctgtaaaag aagttaatca agttgataaa gcacttttaa
     9721 aacaaattgg tgcagttgat gtcttagaag tgaagggtgg cattcaagca atctatggag
     9781 caaaagcaat cttatataaa aatagtatta atgaaatttt aggtgtagat gattaagtac
     9841 ttactgactt aataaaaaac agaggagagt gatggatgag taggatgaaa tgaaatcgca
     9901 tacaagaaat aaagaactca ttatccaagt tggatacgct tattacatag gagaatacaa
     9961 atgaaattta gaaaattagc ttgtacagta cttgcgggtg ctgcggttct tggtcttgct
    10021 gcttgtggca attctggcgg aagtaaagat gctgccaaat caggtggtga cggtgccaaa
    10081 acagaaatca cttggtgggc attcccagta tttacccaag aaaaaactgg tgacggtgtt
    10141 ggaacttatg aaaaatcaat catcgaagcg tttgaaaaag caaacccaga tataaaagtg
    10201 aaattggaaa ccatcgactt caagtcaggt cctgaaaaaa tcacaacagc catcgaagca
    10261 ggaacagctc cagacgtact ctttgatgca ccaggacgta tcatccaata cggtaaaaac
    10321 ggtaaattgg ctgagttgaa tgacctcttc acagatgaat ttgttaaaga tgtcaacaat
    10381 gaaaacatcg tacaagcaag taaagctgga gacaaggctt atatgtatcc gattagttct
    10441 gccccattct acatggcaat gaacaagaaa atgttagaag atgctggagt agcaaacctt
    10501 gtaaaagaag gttggacaac tgatgatttt gaaaaagtat tgaaagcact taaagacaag
    10561 ggttacacac caggttcatt gttcagttct ggtcaagggg gagaccaagg aacacgtgcc
    10621 tttatctcta acctttatag cggttctgta acagatgaaa aagttagcaa atatacaact
    10681 gatgatccta aattcgtcaa aggtcttgaa aaagcaacta gctggattaa agacaatttg
    10741 atcaataatg gttcacaatt tgacggtggg gcagatatcc aaaactttgc caacggtcaa
    10801 acatcttaca caatcctttg ggcaccagct caaaatggta tccaagctaa acttttagaa
    10861 gcaagtaagg tagaagtggt agaagtacca ttcccatcag acgaaggtaa gccagctctt
    10921 gaatatcttg taaacgggtt tgcagtattc aacaataaag acgacaagaa agtcgctgca
    10981 tctaagaaat tcatccagtt tatcgcagat gacaaagagt ggggacctaa agacgtagtt
    11041 cgtacaggtg ctttcccagt ccgtacttca tttggaaaac tttatgaaga caaacgcatg
    11101 gaaacaatca gcggctggac tcaatactac tcaccatact acaacactat tgatggattt
    11161 gctgaaatga gaacactttg gttcccaatg ttgcaatctg tatcaaatgg tgacgaaaaa
    11221 ccagcagatg ctttgaaagc cttcactgaa aaagcgaacg aaacaatcaa aaaagctatg
    11281 aaacaatagt ccttagttat tctataaaaa gtagtttttt aaagaaccta agagtgtata
    11341 cccccttttc cctctacaca gatagtgtaa gaaaaggggg cttttgttta aaatgtaaga
    11401 aactgtcacg aaattaaaat gaagttctta cataagcgaa ccataaaaaa tttcattttg
    11461 attttaaaac agttcaagaa agtcaaaaaa ttattctatt tgaaagagag gtgccgactg
    11521 tgaaagtcaa taaaatccgt atgcgggaaa cagtgatttc ctatgctttc ctagcaccag
    11581 tattattctt ctttgtcatc tttgtgttgg ctccgatggt gatgggcttc attacaagtt
    11641 tctttaacta ctcaatgact aaatttgagt ttgtaggctt ggataactat atccgtatgt
    11701 ttaaagatcc tgtctttaca aaatctctga ttaacacagt tattttggtt attggatctg
    11761 taccagttgt tgttctattc tcactctttg tagcatctca gacctatcat caaaatgtca
    11821 ttgccagatc cttctaccgt ttcgtcttct tccttcctgt tgtaacgggt agtgttgccg
    11881 tgacagttgt ttggaaatgg atttatgacc cactatcagg gattctaaac tttgtcctta
    11941 agtcaagcca catcatcagc caaaacattt cttggttggg agataaaaac tgggcattga
    12001 tggcgattat gattattctc ttgaccactt cagttggtca gcccatcatc ctttatatcg
    12061 ctgccatggg gaatattgac aattcactgg ttgaagcggc gcgtgttgat ggtgcaactg
    12121 agtttcaagt tttttggaag attaaatggc caagccttct tccaacaact ctttatattg
    12181 caatcatcac aacaattaac tcattccagt gtttcgcctt gattcagctt ttgacatctg
    12241 gtggtccaaa ctactcaaca agtaccttga tgtactacct ttacgaaaaa gccttccaat
    12301 tgacagaata cggctatgcc aacacaattg gtgtcttctt ggcagtcatg attgctatcg
    12361 taagctttgt tcaatttaaa gtacttggaa acgacgtaga atactaaaga aaggagacag
    12421 ctatgcaatc tacagaaaaa aaaccattaa cagcctttac tgttatttca acaatcattt
    12481 tgctcttgtt gactgtgctg ttcatctttc cattctactg gattttgaca ggggcattca
    12541 aatcacaacc tgatacaatt gttattcctc ctcagtggtt ccctaaaatg ccaaccatgg
    12601 aaaacttcca acaactcatg gtgcagaacc ctgccttgca atggatgtgg aactcagtat
    12661 ttatctcatt ggtaaccatg ttcttagttt gtgcaacctc atctctagca ggttatgtat
    12721 tggctaaaaa acgtttctat ggtcaacgca ttctatttgc tatctttatc gctgctatgg
    12781 cgcttccaaa acaagttgtc cttgtaccat tggtacgtat cgtcaacttc atgggaatcc
    12841 acgatactct ctgggcagtt atcttgcctt tgattggatg gccattcggt gtcttcctca
    12901 tgaaacagtt cagtgaaaat atccctacag agttgcttga atcagctaaa atcgacggtt
    12961 gtggtgagat tcgtaccttc tggagtgtag ccttcccgat tgtgaaacca gggtttgcag
    13021 cccttgcaat ctttaccttc atcaatactt ggaatgacta cttcatgcag ttggtaatgt
    13081 tgacttcacg taacaatttg accatctcac ttggggttgc gaccatgcag gctgaaatgg
    13141 caaccaacta tggtttgatt atggcaggag ctgcccttgc tgctgttcca atcgtcacag
    13201 tcttcctagt cttccaaaaa tccttcacac agggtattac tatgggagcg gtcaaaggat
    13261 aatactctgc gaaaatcgaa tgcaaactac gtcagcttca ccttgccata cttaagtatt
    13321 gcctgtggtt agcttcctag tttgttcttc aattttcatt gagtatagga aaatcaatct
    13381 atcaagatac agaagtatat tttatagatt tagagaatat agaagttata agtgtctaca
    13441 aaatggaggg tatgcagtta ctttatgaag ttttgtcaga cacttataaa cttaagaatg
    13501 gttttagtta actatcagaa acgaaggaaa gagtatgatt tttgacgatt tgaaaaacat
    13561 caccttttac aaagggattc atcccaattt agacaaggct atcgactatc tctaccaaca
    13621 tcgtaaagat tcattcgaat taggaaagta tgagattgat ggagataaag tctttctagt
    13681 tgttcaggaa aatgtcctca atcaagttga gaataatcaa tttgaacacc ataagaacta
    13741 tgcagatttg catttgctga tagaagggca tgaatattcg agctacggtt cacgtatcaa
    13801 agacgaggca gtagcattcg acgaagcgag tgacattggc tttgttcatt gtcatgaaca
    13861 ctacccactc ttgttgggtt atcacaattt tgcgattttc ttcccaggtg agccacatca
    13921 gccaactggt tatgcaggca tggaagaaaa ggttcggaaa tatctcttta aaattttgat
    13981 tgattaaaaa taggatgaat tgtttttttg taaagctttg acaatactct accatgaaat
    14041 tgatctttgt gaggtagaga aatgagaata aaatatttaa aaattggtat cttctaagca
    14101 tgctgcaaga gctagtttct tagatggaca gggaattaca gttgatgaga tgtcctggat
    14161 aattaggggc attgtgactg gattgattgg cggacacata aaattaggtg cttatgcagc
    14221 taagtatggt attagtatag cacgatcgat cttaagtagg gcagctgcaa ctgcagcagc
    14281 aagagtagga ttactgacca agatttctgg aaggatttta caaatagctg tgaatgtagc
    14341 tgatgtatat ggtaattttg cctacaatat tgctgcagct tgggatgcac atgataaaat
    14401 ccctaacaat ggtcgtataa acttttaaaa tgcgagaatg aaagcacttt gtatttttta
    14461 ttgtatatgt tagcttggac agtgcttgca atgataatcc gtggaggaat aaatggattt
    14521 gatgaacaca cttggcgtaa tattttagct agtgcgttgt cctcgctata tatacattat
    14581 atatccatag ataaaaatag aaaaagtcca aaagaaaaaa gaaaaaatag atttgttcat
    14641 ggtagggact tatgaaagct ttactgacaa aaaagaaaac agtttacaaa gaaaaatgat
    14701 ggaggagcaa acatggcaca aaaaggagta agccttatca aggcagcatt tgatacagat
    14761 aactttctca tgcgttttag tgagaaggtc ttggacatcg tgacagccaa tcttcttttt
    14821 gtcgtctctt gtttacccat cgtgacgatt ggagtggcta aaatcagcct ctacgagacc
    14881 atgttcgaag ttaagaagag cagacgggtg cctgttttta aaatctatct aagatatttc
    14941 aagcaaaatc tgaaactagg tcttcagctg ggtttaatgg agttaggaat tgtgtttctt
    15001 accctttcag atctctatct tttctggggt caaacagctc tgcccttcca attgctgaaa
    15061 gccatttgtt taggtattct gatttttctt actatcgtga tgctggctag ttaccctatc
    15121 gcggcacgtt atgacctatc ttggaaagaa attcttcaaa aaggattgat gttggctagt
    15181 tttaactttc cttggttctt cctcatgtta gccattcttg tcctcattgt gatggttctt
    15241 tatctgtccg ccttcagtct actcttaggt ggctcagtct tcctactttt tgggtttgga
    15301 ctattggtct ttatccagac tggattgatg gagaaaattt tcgcaaaata ccaataggag
    15361 ctttatttct gaaactactt tcaaaggctc caaacgctat tctataagcg agaaactaaa
    15421 atctaagtgt ctataagata ttgaaagcct tttcggtaaa gtgtatacta aagaagtaaa
    15481 gaaaaatctg ctttagcaga aaataaaaat aataggagaa aatctatgtc agatttgaaa
    15541 aaatacgaag gtgtcattcc agccttctac gcatgttatg atgatcaagg agaagtaagc
    15601 ccagaacgta cgcgtgcctt ggttcaatac ttcattgata aaggtgttca aggtctttat
    15661 gtcaatggtt cttctggtga atgtatctac caaagcgttg aagatcgcaa gttgattttg
    15721 gaagaagtca tggcggtagc caaaggtaaa ttgaccatta ttgcccatgt tgcttgcaat
    15781 aatactaaag atagtatgga acttgctcgc catgctgaaa gcttgggagt agatgctatt
    15841 gcaacgattc caccaattta tttccgcttg ccagaatact cagttgccaa atactggaac
    15901 gatatcagtt ctgcagctcc aaacacagac tacgtgattt acaacattcc tcaattggca
    15961 ggggttgctt tgactccaag cctttacaca gaaatgttga aaaatcctcg tgttatcggt
    16021 gtgaagaact cttctatgcc agttcaagat atccaaacct ttgtcagcct tggtggagaa
    16081 gaccatatcg tctttaatgg tcctgatgag cagttcctag gaggacgcct catgggggct
    16141 agggctggta tcggtggtac ttatggtgct atgccagaac tcttcttgaa actcaatcag
    16201 ttgattgcgg ataaggacct agaaacagcg cgtgaattgc agtatgctat caacgcaatc
    16261 attggtaaac tcacttctgc tcatggaaat atgtacggtg tcatcaaaga agtcttgaaa
    16321 atcaatgaag gcttgaatat tggatctgtt cgttcaccat tgacaccagt gactgaagaa
    16381 gatcgtccag ttgtagaagc ggctgctgcc ttgattcgtg aaaccaagga gcgcttcctc
    16441 taatctaaaa ggaggtattt atgacatatt acgttgcaat tgatatcggt ggaaccaaca
    16501 tcaagtatgg tttggttgat caagaggggc aacttcttga atcgcatgaa atgccaactg
    16561 aggcgcataa gggtggacct catatcttac aaaagaccaa agatatcgta gctagttatt
    16621 tagaaaaagg cccagtagca ggtgttgcca tatcttctgc tgggatggtg gatccggata
    16681 agggtgagat tttctatgct gggccgcaaa tccctaacta cgcaggcacc cagttcaaaa
    16741 aggaaatcga agaaagcttt actattcctt gtgagattga aaatgatgtc aactgtgcag
    16801 gtcttgctga ggcagtatct ggttcaggca agggagcaag tatgacactt tgcttgacca
    16861 ttggaaccgg tatcggtggt tgtttgatta tggataggaa agtcttccat ggttttagta
    16921 attcagcctg tgaagtcggg tatatgcata tgcaggatgg agcttttcag gatctcgctt
    16981 ctacaacagc tttagtgaaa tatgtagctg aagcccatgg agaagatgtt gatcagtgga
    17041 atggccgacg tatctttaag gaagctactg aaggaaacaa aatctgcatg gaaggcattg
    17101 accgtatggt tgactatcta ggaaaaggtc tggcaaatat ttgctacgtt gccaatccag
    17161 aagtggttat tcttggtggt ggtatcatgg ggcaagaggc tatcctcaaa cctaagatcc
    17221 gcacagcctt gaaagaggct ttggtaccaa gtttagcaga aaaaacacga ttagaatttg
    17281 cccatcacca aaatacagca gggatgttgg gtgcatatta tcattttaag acaaaacaat
    17341 cctagtttgg ctcagccaaa ctaggatttt cttacacgtt tttgtctacg atagccgttg
    17401 agttttttat tttcccagta gctattaaag attttttcct tgctttcgcg attgatttcc
    17461 aaaaagtagg cataaatcaa atcgataaag aagagcatag gaagttgagc ggatattcgt
    17521 tggatatagg agggttggct gtgggtggct acaagaacag tctctgtata ggtctggcta
    17581 tctttattgg gaacacttgt aaagagtaca gtctttgccc ccatctcctt ggcatctaat
    17641 agactatcta aaatagaagg agttgagcct gaaagtgaga agcccagtac tagacaattt
    17701 tcatccatga tgctggttgt ccaggcaaag ccgtcttggt ctgtcaaagc ttcgcagacc
    17761 acacctagtc gcataaaacg taatttcatt tcacgggcga cgaggccaga actccctgtt
    17821 ccaaagaagt agatacgctc agcatcttcg attagctggg caattcgctc tagttggatt
    17881 tcgtcaatca agtcttgtgt ttgttccctc atattgctat aacttctgag gactcgtttg
    17941 gtcagtggac tgtgcttgga gacttggttg gcttgatttt ctgcctgatg ttggtattgg
    18001 aaaataaatt ctcggtagcc agtaaagcca cactttttag caaagcgggt caaagcagct
    18061 tgagaaatat gtaatttttg ggtgacttgt tgagaagata aatcatctgt aatcgtttca
    18121 gcttgcaaaa aatagcgagc gatttcttgc tctaggtctg tcatttcttc aaaatgtgaa
    18181 tcaatgatag ttgcgatatc tggtttgtcc atagggaaag ctcctttaca tgagtcatac
    18241 tggaagacta gatcagggaa tagtcacact tcattataac acataatata aggatagata
    18301 aataaaaacg catctctgtt ttaaaaacga aaaaatcgaa aaagcttctc tcttttccat
    18361 aattttctac tcaaattgtg gtacaattaa gagtaagatt ttaagttaga aatgagactg
    18421 atttgtatga gaaaatttaa cagccattcg attccgattc ggcttaattt attgttttca
    18481 atcgtcattt tactctttat gaccattatt ggtcgtttgt tgtatatgca ggttttgaac
    18541 aaggattttt acgaaaaaaa gctagcctca gctagtcaga ccaagattac aagcagttca
    18601 gcccgtgggg aaatttatga tgctagtgga aaacctttgg tagaaaatac gttaaagcag
    18661 gttgtttcct ttacgcgtag caataaaatg acggctacag acttaaaaga aacagctaaa
    18721 aagttactga cttatgtgag catcagttct ccaaatttga cagaacgcca gctggcggat
    18781 tactatttgg ctgatcctga aatctataaa aaaatagtga aagctctccc aagtgagaaa
    18841 cgcttggatt cagatggcaa tcgtctatcc gaatcagaac tgtataacaa tgcggtcgat
    18901 agtgtacaaa cgagtcaact aaattataca gaggatgaaa agaaagaaat ctatcttttt
    18961 agtcagttaa atgctgttgg aaactttgcg acaggaacca ttgcgacaga tcctctaaat
    19021 gattctcagg tggctgttat tgcctctatt tcaaaggaga tgcctggcat tagtatttct
    19081 acttcttggg atagaaaggt tttggaaact tccctttctt ctatagttgg gagtgtatcc
    19141 agtgaaaaag ctggtctccc agcggaagaa gcagaagcct atcttaaaaa aggctattct
    19201 ctaaatgatc gtgtaggaac ctcctatttg gaaaagcaat atgaagagac cttacaagga
    19261 aaacgctcgg taaaagaaat ccatctggat aaatatggaa atatggaaag cgtggataca
    19321 attgaggaag gtagtaaggg aaacaatatc aaactgacca ttgatttggc cttccaagat
    19381 agcgtggatg ctttactgaa aagttatttc aattccgagc tagaaaatgg tggagccaag
    19441 tattctgaag gtgtctatgc agtcgccctt aacccaaaaa caggtgcggt tttgtctatg
    19501 tcagggatta aacatgactt gaaaacggga gagttgacgc ctgattcctt gggaacggta
    19561 accaatgtct ttgttccagg ttcggttgtc aaggcggcga ccatcagctc aggttgggaa
    19621 aatggagtct tgtcaggaaa ccagaccttg acagaccagt ccattgtctt ccaaggttca
    19681 gctcccatca attcttggta tactcaggct tacggttcat tccctatcac agcggtccaa
    19741 gctctggagt attcatcaaa tacctatatg gtccaaacag ccttaggtct tatggggcaa
    19801 acctatcaac ccaatatgtt tgtcggcacc agcaatctag agtctgctat ggagaaactg
    19861 cgttcaacct ttggcgaata tggcttgggt actgcgacag gaattgacct accagatgaa
    19921 tctactggat ttgttcccaa agagtatagc tttgctaatt acattactaa tgcctttggg
    19981 cagtttgata actatacgcc gatgcagttg gctcagtatg tagcaactat tgcaaataat
    20041 ggtgttcgtg tggctcctcg tattgttgaa ggcatttatg gtaataatga taagggagga
    20101 ctgggtgact tgattcagca actgcaaccg acagagatga ataaggtcaa tatatccgac
    20161 tccgatatga gcatcttgca ccaaggtttt tatcaggttg cccatggtac tagtggattg
    20221 acaactggac gtgccttttc aaatggtgcc ttggtatcca ttagcggaaa aacaggtaca
    20281 gccgaaagct atgtggcaga tggtcagcaa gcaaccaata ccaatgcggt ggcctatgcc
    20341 ccatatgata atccccaaat cgctgtcgca gtggtctttc ctcataatac caatctaaca
    20401 aatggtgtag gaccttccat tgcgcgtgac attatcaatc tgtatcaaaa ataccatcca
    20461 atgaactaga aaggaaatta tgctttatcc aacacctatt gccaagttga ttgacagtta
    20521 ttctaagtta ccaggtatcg ggattaagac ggctacgcgt ctggcctttt atacgattgg
    20581 gatgtctgct gatgatgtca atgaatttgc aaaaaatctc ctttctgcta agagagaatt
    20641 gacatattgt tctatttgtg gacgtttgac agacgacgat ccttgttcta tctgtactga
    20701 tccgactcgt gaccagacaa caattttagt tcttgaggat agtagagatg tggcagccat
    20761 ggaaaatatc caagaatacc atggactcta tcatgtcctt catggcctca tttctcctat
    20821 gaatggtatc agtccggacg atatcaatct caagagcctt atgactcgtc ttatggatag
    20881 tgaggtttca gaagtgattg tggcgactaa tgctacagcg gatggtgaag cgacttccat
    20941 gtatctttca cgtttgctca agccggctgg tatcaaggtt acgcgtctag cacgaggtct
    21001 cgctgtggga gcggacattg agtatgcgga cgaagtgaca ctcttacgag cgattgaaaa
    21061 tcggacagag ttgtaagtgt aggcaaattt acgaactccc ttcatttata aaaaatcaaa
    21121 gaggctgaaa atcgttccta tcggcctctt ttcgtatagt gtgatgagta ggatcaggtt
    21181 caagttttaa aaaaccaagc aaatatgata tactaaagag cgagtattct agtagaatta
    21241 ggacaaataa tatgaaacaa acgattattc ttttatatgg tggacggagt gcggaacgcg
    21301 aagtctctgt cctttcagct gagagtgtca tgcgtgcggt caattacgac cgtttcacag
    21361 tcaagacttt ctttatcagt cagtcaggtg actttatcaa aacgcaggaa tttagtcatg
    21421 ctccggggca agaagaccgt ctcatgacca atgaaaccat tgattgggat aagaaagttg
    21481 caccaagtgc tatctacgaa gaaggtgcag tggtctttcc agtccttcac gggccaatgg
    21541 gagaagatgg ctctgttcaa ggattcttgg aagttttgaa aatgccttac gttggttgca
    21601 acattttgtc atcaagtctt gccatggata aaatcacgac taagcgtgtt ctggaatctg
    21661 ctggtattgc ccaagttcct tatgtggcta tcgttgaagg cgatgatgtg actgctaaaa
    21721 tcgctgaagt ggaagaaaaa ttggcttatc cagtcttcac taagccgtca aacatggggt
    21781 ctagtgtcgg tatttctagg tctgaaaacc aagaagaact ccgtcaagcc ttaaaacttg
    21841 ccttccgata tgacagccgt gtcttggttg agcaaggagt gaatgcccgt gaaattgagg
    21901 ttggcctctt gggtaactac gatgtcaaga gcacgctacc tggagaagtt gtcaaggacg
    21961 ttgcctttta tgactacgat gccaagtata ttgataacaa ggttactatg gatattcctg
    22021 ccaaaatcag tgatgatgtg gtggctgtca tgcgtcaaaa tgcagaaaca gccttccgtg
    22081 ccattggtgg ccttggtcta tctcgttgcg atttcttcta tacagataag ggagagattt
    22141 ttctcaacga gctcaatacc atgccaggtt ttacccagtg gtctatgtac ccactacttt
    22201 gggacaatat ggggatcagc tacccagaac taatcgagcg tttggttgac cttgccaagg
    22261 aaagttttga caagcgcgaa gcgcatttga tataaaatga aagagagggt agaagccaga
    22321 accatcactg caaggtgact agagttctcg gacttcagcc ctttttaaag gagtagaaat
    22381 gaaattaaca atccatgaaa ttgcccaagt tgttggagcc aaaaatgata tcagtatctt
    22441 tgaggacacc cagttagaaa aagctgagtt tgatagtcgt ttgattggaa ctggagattt
    22501 atttgtgcca cttaaaggtg cgcgtgatgg ccatgacttt attgaaacag cctttgaaaa
    22561 tggtgcagca gtaaccttgt ctgagaaaga ggtctcaaat catccttaca ttctagtaga
    22621 tgatgttttg acagcctttc aatccctagc atcctactat cttgaaaaaa cgactgttga
    22681 tgtctttgct gttacaggtt caaatggcaa gacaacgact aaggatatgt tggcgcattt
    22741 actgtcaacg acctacaaga cctacaaaac acaaggcaac tacaataacg agattggcct
    22801 tccctacaca gttctacaca tgcctgaagg aacagaaaag ttggttttgg agatggggca
    22861 ggaccacttg ggcgatattc atctcttgtc tgaattggct cgtccaaaaa cagccatcgt
    22921 gaccttggtt ggagaagccc atttggcctt tttcaaagac cgttcagaga ttgctaaggg
    22981 aaaaatgcaa attgcagacg gaatggcttc aggttccttg cttttagtgc cggctgaccc
    23041 tatcgtagag gactatttgc caactgataa aaaggtggtc cgttttgggc aaggggcaga
    23101 gttggaaatc acagacttgg ttgagcgtaa ggacagtctg acctttaagg ctaatttctt
    23161 ggaacaagcc cttgatttgc cagtgactgg taagtacaat gctaccaatg ctatgattgc
    23221 atcctatgtt gccctacaag aaggagtttc agaggagcaa attcatcagg ccttccaagg
    23281 tcttgaattg acgcgtaatc gtactgagtg gaagaaagca gccaatggag cagatatcct
    23341 gtcagacgta tataatgcca atccaactgc tatgaagctg attttagaga ctttctctgc
    23401 cattccagcc aatgaaggtg gcaagaaaat tgcagtgttg gcggatatga aggagcttgg
    23461 tgaccagtct attcaactcc ataaccagat gattttgagc ctttctccag atgtgcttga
    23521 taccgttatt ttctatggag aagacattgc tgaattagcc caattggcca gtcaaatgtt
    23581 cccaattggc cacgtttact acttcaagaa aacagaagac caagaccaat ttgaagacct
    23641 agtcaagcag gtcaaggaaa gcctcagtgc caatgatcaa atcttgctca agggctctaa
    23701 ctctatgaac ctagccatgt tggtagaaag tttagaaaat gaaaccaagt gattttgtta
    23761 agtatctgca aagaatgatt gcccttacag atactggctt aacctttaca aaagatcctt
    23821 ttgaccgtga gcgctacgag gacttgcgaa gtctgttatc tgaaatgttg aatcagggat
    23881 cagacctgga tgcagaagaa gtagcagaag tcttgaaacc aacttcagct tatgcgactc
    23941 ccttaatgga cgtccgtgct tggattgttg aggatgagaa gatttgtctg gttaggggac
    24001 aaggagagga tagttgggct ttgccaggtg gctttggtga agtcggctat tctccaactg
    24061 aaaatattct caaggaaatt gaagaagaaa ccggttttaa agccaaagtt gaaagactgc
    24121 tagctgtttt tgataccaat cgtttccaac tacagagcaa acaatatgca aagtttgttt
    24181 ttgaatgtaa gcttctcgat ggacaattcc aagaaaatca agaaatcgct gaccttcaat
    24241 tttttgcaat tgaccaactg ccgaatttat ctgaaaaacg cattaccaag gagcaaatcg
    24301 agattctttg gcaggtttat caaggtcaga tggagcaata tcttgactaa gaagatgatt
    24361 atcgtatttc taaattcatt tttcttaagt agcatggtat aataaaatgc aggaaaattt
    24421 tgaattatga ggaagactag atgaatttat gggatatttt ctttacgact caggcaaccg
    24481 agccgcccaa atttgacctt ttttggtatg ttagcctatt tacgctctta gccttaacca
    24541 tttatacagc ccatcgctat cgtgaaaaga aggtttacca acgatttttc caaatcttgc
    24601 agactgttca gttaatcctt ctttatggtt ggtactgggt caatcatatg ccactgtcag
    24661 aaagcctacc cttttaccat tgccgtatgg ctatgtttgt ggtactcttg cttcctggtc
    24721 agtccaaata taaacaatac tttgcattat tgggaatatt tgggacatta gcagcctttg
    24781 tttatccagt gccagatgct tacccttttc cacatatcac cattttatcc tttatctttg
    24841 gtcatttagc actcttgggg aactctctag tttatctatt gagacagtat aatgcgcgat
    24901 tgctggatgt gaagggaatt tttctcatga cctttgctct aaatgccttg atttttgtgg
    24961 tcaatttggt gacaggcggc gattacggat ttttgacaaa accgccattg gttggggatc
    25021 acggtctagt agctaattat ttacttgttt caattgtgct ggtagctact atcagtttga
    25081 ctaagaaaat cttagaattc tttttagctc aagaagcaga aaaaatgatt gcaaaggaag
    25141 cttaacacag agctttcttt tttgctctta gagagttttt acaagcagct tataaaataa
    25201 gaatttctga atagacaaac tcaaaaaatg gctgggaaat ttaggaaaaa aacaagcacg
    25261 attaaatttt ttgtgttata atattttgtg aatagctatg cctatgttta gctatggaat
    25321 aatacgaagt gcgaaacttg gaagatagag aggaagcgat gtaatggcta gagaaggctt
    25381 ttttacaggt ctagatattg gaacaagctc tgtcaaggtg cttgtggccg agcagagaac
    25441 tggtgaatta aatgtaattg gcgtgagtaa tgccaaaagt aaaggtgtaa aggatggaat
    25501 tattgttgat attgatgcag cagcaactgc tatcaagtca gccatttccc aagcggaaga
    25561 aaaggcaggc atttcgatta aatcagtaaa tgtcggcttg cctggtaatc ttttgcaggt
    25621 agaaccaact caggggatga ttccagtaac atctgatact aaggaaatta cggatcaaga
    25681 tgttgaaaat gttgtcaaat cagctttgac aaagagtatg acacctgacc gtgaagtcat
    25741 tacctttatt cctgaagaat ttattgtgga tggtttccaa gggattcgtg acccacgtgg
    25801 catgatgggg gttcgccttg aaatgcgtgg tttgctttat acaggacctc gtactatctt
    25861 gcacaatttg cgtaagacgg ttgagcgtgc aggtgttcag gttgaaaatg ttatcatttc
    25921 accactagca atggttcagt ctgttttgaa cgaaggggaa cgtgaatttg gtgctacagt
    25981 gattgatatg ggggcaggtc aaacgactgt cgctacaatc cgtaatcaag aactccagtt
    26041 cacacatatt ctccaagaag gtggagatta tgtaactaaa gatatctcca aggttttgaa
    26101 aacctctcgc aaattagcgg aaggcttgaa actgaattac ggggaagcct atccgcctct
    26161 tgcaagcaaa gaaaccttcc aagtagaggt tattggagaa gtagaagcag tcgaagtgac
    26221 ggaagcctac ttgtcagaaa ttatttctgc acgaatcaag cacatccttg aacaaatcaa
    26281 gcaagaatta gatagaaggc gtctattgga cctccctggt ggtattgtct taatcggtgg
    26341 gaatgccatt ttaccaggta tggttgagct tgctcaggaa gtctttggcg tccgtgtcaa
    26401 gctttatgtt ccaaatcaag ttggtatccg taatccagcc tttgcgcatg tgattagttt
    26461 atcagaattt gcgggtcaat taacagaagt taatcttttg gctcagggag cgataaaagg
    26521 tgagaatgac ttaagtcatc agccaattag ttttggtggg atgctgcaaa aaacagctca
    26581 gtttgtacaa tcaacgcctg ttcaaccagc tcctgctcca gaagtagagc cggtggcgcc
    26641 tacagaacca atggcggatt tccaacaagc ttcacaaaat aaaccgaaat tagcagatcg
    26701 tttccgtggc ttgatcggaa gcatgtttga cgaataaaga ggaaaaataa attatgacat
    26761 tttcatttga tacagctgct gctcaagggg cagtgattaa agtaattggt gtcggtggag
    26821 gtggtggcaa tgccatcaac cgtatggtcg acgaaggtgt tacaggcgta gaatttatcg
    26881 cagcaaacac agatgtacaa gcattgagta gtacaaaagc tgagactgtt attcagttgg
    26941 gacctaaatt gactcgtggt ttgggtgcag gaggtcaacc tgaggttggt cgtaaagccg
    27001 ctgaagaaag cgaagaaaca ctgacggaag ctattagtgg tgccgatatg gtcttcatca
    27061 ctgctggtat gggaggaggc tctggaactg gagctgctcc tgttattgct cgtatcgcca
    27121 aagatttagg tgcgcttaca gttggtgttg taacacgtcc ctttggtttt gaaggaagta
    27181 agcgtggaca atttgctgta gaaggaatca atcaacttcg tgagcatgta gacactctat
    27241 tgattatctc aaacaacaat ttgcttgaaa ttgttgataa gaaaacaccg cttttggagg
    27301 ctcttagcga agcggataac gttcttcgtc aaggtgttca agggattacc gatttgatta
    27361 ccaatccagg attgattaac cttgactttg ccgatgtgaa aacggtaatg gcaaacaaag
    27421 ggaatgctct tatgggtatt ggtatcggta gtggagaaga acgtgtggta gaagcggcac
    27481 gtaaggcaat ctattcacca cttcttgaaa caactattga cggtgctgag gatgttatcg
    27541 tcaacgttac tggtggtctt gacttaacct tgattgaggc agaagaggct tcacaaattg
    27601 tgaaccaggc agcaggtcaa ggagtgaaca tctggctcgg tacttcaatt gatgaaagta
    27661 tgcgtgatga aattcgtgta acagttgtcg caacgggtgt tcgtcaagac cgcgtagaaa
    27721 aggttgtggc tccacaagct agatctgcta ctaactaccg tgagacagtg aaaccagctc
    27781 attcacatgg ctttgatcgt cattttgata tggcagaaac agctgaattg ccaaaacaaa
    27841 atccacgtcg tttggaacca actcaggcat ctgcttttgg tgattgggat cttcgccgtg
    27901 aatcgattgt tcgtacaaca gattcagtcg tttctccagt cgagcgcttt gaagccccaa
    27961 tttcacaaga tgaagatgaa ttggatacac ctccattttt caaaaatcgt taagtaaatg
    28021 aatgtaaaag aaaatacaga acttgttttt cgagaagttg cagaggctag tctgagtgct
    28081 aatcgagaga gtggttcggt ctctgtcatt gcagttacca agtatgtaga tgtaccgaca
    28141 gcggaagcct tgcttccgct aggtgttcat catatcggtg aaaatcgtgt agataagttt
    28201 ctggaaaaat atgaagcttt aaaagatcga gatgtgactt ggcatttgat tggtaccttg
    28261 caaagacgta aggtgaaaga tgtcattcaa tacgttgatt atttccatgc attggactca
    28321 gtaaagctag caggggaaat tcaaaaaaga agtgaccgag tcatcaagtg tttccttcaa
    28381 gtaaatattt ctaaagaaga aagcaaacac ggtttttcga gagaggaact gatggaaatc
    28441 ttgccagagt tagccagact agataagatt gaatatgttg gtttaatgac gatggcacct
    28501 tttgaggcta gcagtgagca gttgaaagag attttcaagg cgacccaaga tttacaaaga
    28561 gaaattcaag agaaacaaat tccaaatatg cctatgaccg agttaagtat gggaatgagt
    28621 cgtgattata aagaagcgat tcaattcggt tccacttttg ttcgtatagg tacatcattt
    28681 tttaagtagg agagaaccat gtctttaaaa gatagattcg atagatttat agattatttt
    28741 acggagaatg aggattcaag tctcccttat gaaaaaagag atgagcctgt gtttactcca
    28801 gtaaattctt cacaggaacc ggctctccca atgaatcaac cttcacagtc ggctggtaca
    28861 aaagagaaca atatcaccag acttcatgca agacaacagg aattggcaaa tcagagtcag
    28921 cgtgcaacgg ataaggtcat tatagatgtt cgttatccta gaaaatatga ggatgcaaca
    28981 gaaattgttg atttattggc aggaaacgaa agtatcttga ttgattttca gtatatgaca
    29041 gaggtgcagg ctcgtcgttg tttggactat ttggatggag cttgtcatgt tttagctgga
    29101 aatttgaaaa aggtagcttc taccatgtat ttgttgacac cagtgaatgt tattgtaaat
    29161 gttgaagata tccgtttacc agatgaagat caacagggtg agttcggttt tgatatgaag
    29221 cgaaatagag tacgataatg atttttttaa ttcgtatgat ttataatgca gtggatattt
    29281 actccctgat tttggtagcc ttcgctgtca tgtcttggtt tccaggtgcc tacgaatcca
    29341 gtttaggtcg ttggattgta gcgttggtga aaccagtgct tgctcccttg caacgcctgc
    29401 ctttacagat agcgggtctt gatttatcta tttgggtcgc gattgttttg gttcgatttt
    29461 taggagaaaa cctagtgcgt tttctggcga tgataggatg aataaaggga tttatcagca
    29521 tttctccata gaagatcgtc catttcttga caagggaatg gaatggataa agaaggtaga
    29581 agatagctat gctccttttt taactccttt tatcaatcct catcaggaga agctattaaa
    29641 gattttggcc aaaacctatg gtcttgcttg tagcagtagt ggggaattcg tctcgagtga
    29701 gtatgttcga gttttattat acccagatta tttccaacca gagttttcag attttgaaat
    29761 atctctccag gaaattgtgt attccaataa atttgaacat ttaacgcatg ctaagatttt
    29821 agggacagtc atcaatcaat tagggattga acggaaactt tttggagata tcctagtaga
    29881 tgaagaacgg gcgcagatta tgattaatca gcagtttctt cttctctttc aagatggact
    29941 aaagaaaatt ggtcgtatac ctgtttcgct ggaggaacgt cctttcaccg agaaaataga
    30001 taagctagaa cagtatcgag aactggattt atctgtgtct agttttcgat tagatgttct
    30061 tttatcaaat gttttgaaac tatctaggaa tcaagcaaac cagttgattg aaaagaaact
    30121 tgtccaagta aattatcatg tggtagacaa atcagattac actgttcaag ttggagactt
    30181 gattagtgtg agaaaatttg gtcgcttgag attacttcaa gataagggac aaacgaaaaa
    30241 agagaagaaa aaaataaccg tccagttatt attaagtaag tgaggaatag aatgccaatt
    30301 acatcattag aaataaagga caagactttt ggaactcgat tcagaggttt tgatccagaa
    30361 gaagtcgatg aatttttaga tattgtggtt catgattacg aagatcttgt gcgtgcgaat
    30421 catgataaaa atttgcgtat taagagttta gaagagcgtt tgtcttactt tgatgaaata
    30481 aaagattcat tgagccagtc tgtattgatt gctcaggata cagctgagag agtgaaacag
    30541 gcggcgcatg aacgttcaaa caatatcatt catcaagcag agcaagatgc gcaacgcttg
    30601 ttggaagaag ctaaatataa ggcaaacgag attcttcgtc aagcaactga taatgctaag
    30661 aaagtcgctg ttgaaacaga agaattgaag aacaagagcc gtgtcttcca ccaacgtctc
    30721 aaatctacaa ttgagagtca gttggctatt gttgaatctt cagattggga agatcttctc
    30781 cgtccaacag ctacttatct tcaaaccagt gatgaagcct ttaaagaagt ggttagcaaa
    30841 gtacttggag aaccgattcc agctccaatt gaagaagaac caattgatat gacacgtcag
    30901 ttctctcaag cagaaatggc agagttacaa gctcgtattg aggtagccga taaagaattg
    30961 gctgaatttg aagctcagat taaacaggaa gtggaagctc caacttgtag tgagtcctca
    31021 ggttgaagaa gagcctctgc tcatccagtt ggcccaatgt atgaagaacc agaagtagct
    31081 ccagtgcatc cgacaggtcc aacaccagct acagaaactg ttgattcaat accgggattt
    31141 gaagcaccgc aagaatctgt tacaatttta taagaaatat tctgagaaca atatcttatc
    31201 cttatatttc cagcgagcag gaaatggtgt gagtcctgca ttccctatcg ataagattat
    31261 cctctcaaac tatcaagtct gaatctagta agatttgacg ttccccacgt tacgggataa
    31321 gagagagaaa gactaaatct ttttccgaat aaaggtggta ccacgatttt cgtccttttt
    31381 ggaagtcgtg gtttttaatt tgttattatt tataaaggag ataccatgaa actcaaagac
    31441 acccttaatc ttgggaaaac tgaattccca atgcgtgcag gccttcctac caaagagcca
    31501 gtttggcaaa aggaatggga agatgcaaaa ctttatcaac gtcgtcaaga attgaaccaa
    31561 ggaaaacctc atttcacctt gcatgatggc cctccatacg ctaacggaaa tatccacgtt
    31621 ggacatgcta tgaacaagat ttcaaaagat atcattgttc gttctaagtc tatgtcagga
    31681 ttttacgcgc catttattcc tggttgggat actcatggtc tgccaatcga gcaagtcttg
    31741 tcaaaacaag gtgtcaaacg taaagaaatg gacttggttg agtacttgaa actttgccgt
    31801 gagtacgctc tttctcaagt agataaacaa cgtgaagatt ttaaacgttt gggtgtttct
    31861 ggtgactggg aaaatccata tgtgaccttg actcctgact atgaagcagc tcaaattcgt
    31921 gtatttggtg agatggctaa taagggttat atctaccgtg gtgccaagcc agtttactgg
    31981 tcatggtcat ctgagtcagc ccttgctgaa gcagagattg aataccatga cttggtttca
    32041 acttcccttt actatgccaa caaggtaaaa gatggcaaag gagttctaga tacagatact
    32101 tatatcgttg tctggacaac gactccattt accatcacag cttctcgtgg tttgacggtt
    32161 ggtgcagata ttgattacgt tttggttcaa cctgctggtg aagctcgtaa gtttgtcgtt
    32221 gctgctgaat tattgactag cttgtctgag aaatttggct gggctgatgt tcaagttttg
    32281 gaaacttacc gtggccaaga acttaaccac atcgtaacag aacacccatg ggatacagct
    32341 gtagaagagt tggtaattct tggtgaccac gttacgactg actctggtac aggtattgtc
    32401 catacagccc ctggttttgg tgaggacgac tacaatgttg gtattgctaa taatcttgaa
    32461 gtcgcagtga ctgttgatga acgtggtatc atgatgaaga atgctggtcc tgagtttgaa
    32521 ggtcaattct atgaaaaggt agttccaact gttattgaaa aacttggtaa cctccttctt
    32581 gcccaagaag aaatctctca ctcatatcca tttgactggc gtactaagaa accaatcatc
    32641 tggcgtgcag ttccacaatg gtttgcctca gtttctaaat tccgtcaaga aatcttggac
    32701 gaaattgaaa aagtgaaatt ccactcagaa tggggtaaag tccgtcttta caatatgatc
    32761 cgtgaccgtg gtgactgggt tatctctcgt caacgtgctt ggggtgttcc acttccaatc
    32821 ttctatgcag aagacggtac agctatcatg gtagctgaaa cgattgaaca cgtagctcaa
    32881 ctttttgaag aacatggttc aagcatttgg tgggaacgtg atgccaaaga tctcttgcca
    32941 gaaggattta ctcatccagg ttcaccaaac ggcgagttca aaaaagaaac tgatatcatg
    33001 gacgtttggt ttgactcagg ttcatcatgg aatggagtgg tggtaaaccg tcctgaattg
    33061 acttacccag ccgaccttta cctagaaggt tctgaccaat accgtggttg gtttaactca
    33121 tcacttatca catctgttgc caaccatggc gtagcacctt acaaacaaat cttgtcacaa
    33181 ggttttgccc ttgatggtaa aggtgagaag atgtctaaat ctcttggaaa taccattgct
    33241 ccaagcgatg ttgaaaaaca attcggtgct gaaatcttgc gtctctgggt aacaagtgtt
    33301 gactcaagca atgacgtgcg tatctctatg gatattttga gccaagtttc tgaaacttac
    33361 cgtaagattc gtaacactct tcgtttcttg attgccaata catctgactt taacccagct
    33421 caagatacag tcgcttacga tgagcttcgt tcagttgata agtacatgac gattcgcttt
    33481 aaccagcttg tcaagaccat tcgtgatgcc tatgcagact ttgaattctt gacgatctac
    33541 aaggccttgg tgaactttat caacgttgac ttgtcagcct tctaccttga ttttgccaaa
    33601 gatgttgttt acattgaagg tgccaaatca ctggaacgcc gtcaaatgca gactgtcttc
    33661 tatgacattc ttgtcaaaat caccaaactc ttgacaccaa tccttcctca cactgcggaa
    33721 gaaatttggt catatcttga gtttgaaaca gaagacttcg tccaattgtc agaattacca
    33781 gaggctcaaa cttttgctaa tcaagaagaa atcttggata catgggcagc cttcatggac
    33841 ttccgtggac aagctcaaaa agccttggaa gaagctcgta atgcaaaagt aatcggtaaa
    33901 tcacttgaag cacacttgac agtttatcca aacgaagttg tgaaaactct actcgaagca
    33961 gtaaacagca atgtggctca acttttgatc gtgtcagact tgaccatcgc agaaggacca
    34021 gctccagaag ctgcccttag cttcgaagat gtagccttca cagttgaacg cgctgcaggt
    34081 gaagtatgtg accgttgccg tcgtattgac ccaacaacag cagaacgtag ctaccaggca
    34141 gttatctgtg accactgtgc aagcatcgta gaagaaaact ttgcggaagc agtcgcagaa
    34201 ggatttgaag agaaataaga ttgaaaagtc taggcaaaat tcaatttgag aagaaaagac
    34261 aactaatttt atagtctatt aaacgcattg tatcacgttt ttgaacacct gatatgatgc
    34321 gttttttatt tattttaaaa atttgcgagg tatgactttt tatactcaac aagaatcaaa
    34381 gagaaactta gcaagctaac agtagtaaga taaaatagga atttgatatt agggataaga
    34441 ttggtaaata gt
//
LOCUS       DAVKAO010000015        34022 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKAO010000015 DAVKAO010000000
VERSION     DAVKAO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34022
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.015"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..264
                     /locus_tag="VWX16_000754"
     CDS             <1..264
                     /locus_tag="VWX16_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158142.1"
                     /translation="KLGITLMFVRIQMLISIGLLVLTWTSWFKNLFPIFENNQVARLF
                     VFVVLLLGLVLSLLDIRRIKKIYKRTDKVYQQFVQLEKNSLSL"
     gene            344..1690
                     /gene="celB"
                     /locus_tag="VWX16_000755"
     CDS             344..1690
                     /gene="celB"
                     /locus_tag="VWX16_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU9158143.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            2070..2246
                     /locus_tag="VWX16_000756"
     CDS             2070..2246
                     /locus_tag="VWX16_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158144.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFRSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            2647..4860
                     /locus_tag="VWX16_000757"
     CDS             2647..4860
                     /locus_tag="VWX16_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9158145.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDSLWLLNHYHYQDSCKNAEGGLTLSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDVSNI
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(5082..5558)
                     /locus_tag="VWX16_000758"
     CDS             complement(5082..5558)
                     /locus_tag="VWX16_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9158146.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            5781..8984
                     /locus_tag="VWX16_000759"
                     /pseudo
     CDS             5781..8984
                     /locus_tag="VWX16_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
     gene            9365..9741
                     /gene="tnpA"
                     /locus_tag="VWX16_000760"
                     /pseudo
     CDS             9365..9741
                     /gene="tnpA"
                     /locus_tag="VWX16_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10129..10758)
                     /locus_tag="VWX16_000761"
     CDS             complement(10129..10758)
                     /locus_tag="VWX16_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9158147.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDKSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPSSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(10768..11769)
                     /locus_tag="VWX16_000762"
     CDS             complement(10768..11769)
                     /locus_tag="VWX16_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9158148.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIQPNMLDMDSLFQGISHFHFSGITVAIGQEVRTILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKTIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(11800..12441)
                     /locus_tag="VWX16_000763"
     CDS             complement(11800..12441)
                     /locus_tag="VWX16_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9158149.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(12460..13275)
                     /locus_tag="VWX16_000764"
     CDS             complement(12460..13275)
                     /locus_tag="VWX16_000764"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9158150.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            13547..13981
                     /locus_tag="VWX16_000765"
     CDS             13547..13981
                     /locus_tag="VWX16_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9158151.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            13993..15183
                     /locus_tag="VWX16_000766"
     CDS             13993..15183
                     /locus_tag="VWX16_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9158152.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELAHHDLGFLYTPSCMAEYKINEDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGMTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            15193..15684
                     /locus_tag="VWX16_000767"
     CDS             15193..15684
                     /locus_tag="VWX16_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9158153.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKVALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            15699..16478
                     /locus_tag="VWX16_000768"
     CDS             15699..16478
                     /locus_tag="VWX16_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9158154.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            16465..17283
                     /locus_tag="VWX16_000769"
     CDS             16465..17283
                     /locus_tag="VWX16_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9158155.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            17283..17576
                     /locus_tag="VWX16_000770"
     CDS             17283..17576
                     /locus_tag="VWX16_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9158156.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            17598..19499
                     /locus_tag="VWX16_000771"
     CDS             17598..19499
                     /locus_tag="VWX16_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9158157.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            19559..20560
                     /locus_tag="VWX16_000772"
     CDS             19559..20560
                     /locus_tag="VWX16_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9158158.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQSGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(20670..21005)
                     /locus_tag="VWX16_000773"
     CDS             complement(20670..21005)
                     /locus_tag="VWX16_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158159.1"
                     /translation="MKSLARLLNIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(21016..21258)
                     /locus_tag="VWX16_000774"
     CDS             complement(21016..21258)
                     /locus_tag="VWX16_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158160.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(21269..21811)
                     /locus_tag="VWX16_000775"
     CDS             complement(21269..21811)
                     /locus_tag="VWX16_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9158161.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(21827..22021)
                     /locus_tag="VWX16_000776"
     CDS             complement(21827..22021)
                     /locus_tag="VWX16_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9158162.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            22187..23137
                     /gene="rsmH"
                     /locus_tag="VWX16_000777"
     CDS             22187..23137
                     /gene="rsmH"
                     /locus_tag="VWX16_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9158163.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            23149..23466
                     /gene="ftsL"
                     /locus_tag="VWX16_000778"
     CDS             23149..23466
                     /gene="ftsL"
                     /locus_tag="VWX16_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9158164.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            23470..25722
                     /gene="pbpX"
                     /locus_tag="VWX16_000779"
     CDS             23470..25722
                     /gene="pbpX"
                     /locus_tag="VWX16_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9158165.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            25724..26704
                     /locus_tag="VWX16_000780"
     CDS             25724..26704
                     /locus_tag="VWX16_000780"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9158166.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLFTSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLSFFIFNHKP
                     AKIFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            27313..29418
                     /locus_tag="VWX16_000781"
     CDS             27313..29418
                     /locus_tag="VWX16_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9158167.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRTTGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTCGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQVAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(29714..30196)
                     /locus_tag="VWX16_000782"
     CDS             complement(29714..30196)
                     /locus_tag="VWX16_000782"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9158168.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(30291..31775)
                     /locus_tag="VWX16_000783"
     CDS             complement(30291..31775)
                     /locus_tag="VWX16_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9158169.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYTGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKISRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            31927..33534
                     /locus_tag="VWX16_000784"
     CDS             31927..33534
                     /locus_tag="VWX16_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9158170.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDELIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQVTFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPMQEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            33693..33866
                     /locus_tag="VWX16_000785"
     CDS             33693..33866
                     /locus_tag="VWX16_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU9158171.1"
                     /translation="MNIKKRVLGAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(33859..>34022)
                     /locus_tag="VWX16_000786"
     CDS             complement(33859..>34022)
                     /locus_tag="VWX16_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9158172.1"
                     /translation="EEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCCNTFYE
                     ALLSCSCFN"
BASE COUNT        10434 a         5953 c         7524 g        10111 t
ORIGIN      
        1 aagttgggaa taacactaat gtttgttaga atacaaatgc ttatttcaat aggattactt
       61 gttttaactt ggacaagttg gtttaagaat ctttttccga tttttgaaaa taaccaagta
      121 gcacgtttat ttgtttttgt agttcttcta ttaggtttag ttctgagttt gttagacatt
      181 agaagaatta aaaaaattta taaacgaaca gataaggttt atcaacaatt tgtccaattg
      241 gaaaagaact cacttagttt ataatcctga tctagtataa tttgtatttg aaactagtct
      301 ttagaaagta aaaattatta tgttttatta aggaggtcta tttatggatc aacaaaacgg
      361 gttgtttggt tttcttgaaa accatgttat gggaccaatg ggcaaacttg ctcagtttaa
      421 agtagtacgt gctatcacgg ctgcaggtat ggctgctgta ccatttacta ttgtaggatc
      481 aatgtttttg gtattcagta ttttgccaca agctttctca ttttggccaa ttgtggcaga
      541 tattttctct gcttcatttg ataaattcac atcactttac atggttgcaa actatgcgac
      601 tatgggttct ctatctcttt atttcgttct atcacttgca tatgaattga caaaaattta
      661 tgcagaggaa gaagaactca atatgaatcc tcttaatggt gccttgcttg ccttgatggc
      721 ttttgtcatg acagtaccgc aaatcatttt tgatggtgga atgatgaaga ctgtgacaag
      781 tctaaaagaa ggtgcagtaa ttgcagatgg atgggcaatg ggaaatggag tcgcacgttt
      841 tgggacaaca gggattttta ccgcaatcat tatggcaatt gtgactgttc ttatttatcg
      901 tatgtgtgtt aaacataatt gggttattaa aatgcctgaa gctgttccag aaggagtttc
      961 tcgtggattt accgctttgg ttccgggatt tgttgttgca tttgttgtta tctttatcaa
     1021 cggtcttctt gtagcaatgg gaacagatat ttttaaagtc attgcaattc catttggttt
     1081 tgtatccaat ctgactaatt cgtggattgg tttaatgatt atttatctat tgactcaact
     1141 actttggatt gtaggtatcc acggtgcgaa cattgttttt gcatttgtta gtccaattgc
     1201 tcttgctaac atggctgaaa atgctgctgg cgggcacttc gctgttgcag gtgaattttc
     1261 taatatgttt gtaattgcag gtggttctgg tgcaacttta ggactatgtt tatatattgc
     1321 ttttgcctct aaatctgaac agcttaaagc aataggacga gcatctgtag ttccggcctt
     1381 atttaatatt aatgaaccat taatttttgg attacctatt atctataatc cagccttagc
     1441 tataccattt attttagcac caatggttac tgctactatt tattacgtag cgaattctct
     1501 aaactttatt aagccaatta tcgcacaggt tccatggcca actccagtag ggattggagc
     1561 tttcttaggg acagcagatc ttcgagctgt attagttgct ctagtatgtg catttgcagc
     1621 attcctagtc tatcttccat tcatccgtgt atatgatcaa aaattggtga aagaagagca
     1681 aggtatctaa taagtaaaaa agtatactcg atgatagaat ccatatagtt taaataatga
     1741 gcgttaacat tagcagtgaa gaggtctttt taagatattc ttctctgctt tttcatattt
     1801 tttcgctatg tagggtatga agcaaaatag aaagtagttc gaatagttga taaacaggga
     1861 attgctgtta tttaaaaaaa acaataaagt aaagtatgtt atcgtagaat acgatacaat
     1921 agtaaaaaag caaaaagaat gcgttttata tttgggtttg aggtttataa aataggataa
     1981 gcatgctgaa aacttagata tgacaaacaa aataaaattt ttaaaagcta catagaaaca
     2041 tggtataatg tatctaaagg aggtagcata tgtccttaga tatagataaa gaaaagatga
     2101 caatcatggg aatagctttt gaaaaccgat ccgtttttag gagtgtttgg tatgctttaa
     2161 gtacaaatat gattgaagga tggcgaccta cggttagtga tgttgaaaaa ttacgagatg
     2221 aagctcttgc tctggggatg acttaatgaa gcgcaactgt tatgagtctt atgactatgt
     2281 tgatccggat aatttatata ccgatccggg ttcttcagtt ctaagaaata agcaggaagg
     2341 aaaaaagcac gcgagcttga atatcgtatg attgctagta agagtttgaa attatttatc
     2401 aatcctatct tagttaatag tttaaaagta gtttgtttca caactgcata agcttagaaa
     2461 agttgaaaag tgataactct ttaataaact cttggtttgt taaaagatat tttgtccact
     2521 acattttttc aattgactat tatttgtcat agatataaca gatagaacat tgcattcaga
     2581 ataatgaaaa acgcatcaaa aatggtgcgt tctgttttta tgctaccata aaaggaggaa
     2641 aacaaattga aaatttttaa gggagagttt tatcgaatct ctgtattaac agacaagcta
     2701 gtaaggttag aatactctcg aactggaaat tttgaggata gaacgacaca acttatctat
     2761 aatagagatt ttggccaagt ttcgttagat tatatcgaga catcaaatgt actagatatt
     2821 atgacggact attttcatct gcactttaat aaaggagaat ttaacgccga aaatttattt
     2881 atagaattaa aaggaaattt tgccgtatat ggtagtcgct ggtattttgg tgaatctatt
     2941 gaaacgttaa aaggaacagc tcggactctg gataaggcag atggagcaat ctcgttagaa
     3001 gatggaatta ttagccgaaa tggtatagcc ttattggatg attctcaagg atttatttgg
     3061 gatgaacaat ctggttatat tgagagagaa aatcaaattg acctgtattt ctttgcctat
     3121 gggcatgatt atagaggagc aatcagagac ttttaccatt tgactggttc aacacccttg
     3181 ttgccaagat atgctttagg caattggtgg agtagatatt ggccttatac gtcggatgaa
     3241 tacttggatt taatagacag atttgaaaca gagaaaattc cattatctat cggtgtgtta
     3301 gatatggatt ggcatataac tgacattcca gctcgttttg gaagtggctg gacaggatat
     3361 agttggaata gaaacttaat accaaatcca gaacagttat tgcaacaact tcatgataga
     3421 aagttaaaac tctccttaaa tgtccatcct gctgatggga tacgggctta tgaagaagct
     3481 tatcctcaaa tcgcaaaacg gttggggtta aatgtagaac tagaagaacc tgctattttt
     3541 gattttttta atccctcttt tagggaagcc tactttaaag atgttcatta tgagctagaa
     3601 aagcagggag tagatttttg gtggattgac tggcaacaag ggacacaagg tatgctagat
     3661 tcactttggc ttttaaacca ttatcactat caggatagtt gtaaaaatgc agaaggtggt
     3721 ttgactttat caagatatgc aggtcctggt agtcaccgct accctgttgg tttttcaggg
     3781 gatactatta ttagttggaa ttccttaaga tttcaaccct attttacagc gacagcatct
     3841 aatatcggtt atagttggtg gagtcatgat atcggtggac atatgttggg ggattatgac
     3901 gaagagctac aaactagatg gctacagttt ggtgttttta gtccgattac tcgattacat
     3961 agttctagaa gtccttttaa tagtaaagaa ccttggtttt tttcagaaac aacatctaag
     4021 attatgaaga aataccttcg tttgagacat cagatgattc cctatctata taccatgaat
     4081 gtacagacac atgaggaagg tgccccatta atcagtccaa tctattattt ctatccagag
     4141 aatgatgaga gctataatgt tccaaaccaa tacttttttg gaacagaact gatggtggct
     4201 cccattgtag aaaagatgga tttgacattc caatctgcaa aagtagatgt atggttccct
     4261 gaaggtgaat ggtatgactt cttttcagag aaaaaataca caggtggtgt gaagttaagt
     4321 gtttataggg acgtttcgaa tattcctgtg tttgcaaaaa gtggtgcaat cattcccttg
     4381 gttggttctg agataggtat gggtgttgat ttacctgagg ttgtagattg gtatgtattc
     4441 ccaggcaaac aacattcttt tgaaatgctt gaagatcaaa atggtcaaag atataaaaca
     4501 agattatcaa tcgattggga aatgggaatg gtagagttag cattacaagg agattctagt
     4561 atcgttccaa gcaatagaaa acatagaatt cattttaaag gaacgaatgt gtctataatt
     4621 gaattgccaa ataagaatga tacagctaaa tttgaatgga aagataataa aaggacatct
     4681 ttaaatgacg aagtttttag actactaaag acagcttctc ttccatatga attaaaagat
     4741 agattgttaa atcaattcat caatgcgaaa aactctcatg acttaatgaa tatcttgcat
     4801 catcaggata aggaattgag agggcgtttg ttggaaatga tatttactag ccaaaactaa
     4861 ttgatataat tagaaagtca tttgtttagt gtatatgaag attctgtatg gtattcgtta
     4921 tcactggtca cattgaaaat gtgaaataag cacctacaac atagacttta caacacttca
     4981 tgtttcatat catggggtgt ttttctattt aagtcaaagc caatcaaaaa atctcactat
     5041 tcatcaggtt aaaggaaacc taatcatagt gagattgtga attatagtag agtttgtatc
     5101 gcctcttcaa tttgttttgg gtctgttttt gaagaaaagc gttcaaagac ttgcccatca
     5161 cgaccaatga gaaacttagc gaaattccat tcgattcgtt ttcctagtgg gccagattta
     5221 tggtctttta accagacata gagagggtct gcttccttac cgttgacctt aatcttggca
     5281 aaacgtggga aggtggtttg aaaatgtagg ctacagaagg cgttgatttc ctctgcgctg
     5341 ccgggtgctt gtcccataaa ctgattgcaa gggaaatcca atatttcgaa gccctgatct
     5401 tgatagcgtt catagagttc ttgaagtccc tggtactggg gcgttaaacc acatccagta
     5461 gcagtgttga caatcaagag aactttgcca cgatagctat ccaagggagt tgcttggttg
     5521 ttttggttca aaacggaaaa atcataaagt gaagtcatga gggcctcttt tctttttcta
     5581 cattgtaaca gtttttactt ttttcgtcga gtcggatgga agatattgtt gaagaataag
     5641 ccttcaatat gatagaaatc gtttacaaat aactttaaaa atggtatgat ttaattaaac
     5701 cggtttactt tgttgctata aatctctaat ttgagattta gttcctattg attttacaat
     5761 atgtttattg gagtgtatac atgcaaacaa aaacaaagaa gctcattgtg agtttgtctt
     5821 cacttatttt atcaggattt ttattaaacc attatatgac agttggagcg gaagaaacga
     5881 ctacgaatac cattcagcaa agccagaagg aagttcagta tcagcaaagg gatacaaaaa
     5941 atttagttga aaatggtgat tttggtcaga cggaggacgg aagcagtccg tggacaggaa
     6001 gcaaagctca ggggtggtca gcttgggtag accagaagaa tagttctgca gatgcctcaa
     6061 ctcgagtcat tgaggctaag ggtggggcta tcactatctc aagccatgag aaattaaggg
     6121 cagcggttca ccgtatggtt cctattgaag ctaagaaata gtataaactg cgtttcaaga
     6181 ttaaaacaga taataaagtc gggattgcca aggttcgtat cattgaggaa agtggtaagg
     6241 acaagcgatt gtggaattct gcaacgacgt caggaacaaa ggactggcag accattgaag
     6301 cagactatag cccgacttta gatgttgata aaatcaagct ggagttattc tatgaaacag
     6361 gaactgggac tgtttccttt aaggatattg agctggtaga ggtagcagac cagccttctg
     6421 aggattctca aacagataaa cagcttgagg aaaagattga tttaccaatt ggaaaaaaac
     6481 atgttttttc tcttgcggac tatacttata aggtagaaag tcctgacgtt gcttcagtca
     6541 aaaatggaat tttagaacct cttaaggaag ggacaaccaa tgtcattgtc agtaaagatg
     6601 gcaaggaagt gaaaaagatt cctttgaaga ttctagcctc tgttaaggat gcatacacag
     6661 accgtttgga tgactggaat ggcatcatcg ctgggaatca atactatgat tctaaaaatg
     6721 aacagatggc caaattaaac caggaattgg aaggaaaggt agctgatagc ctatctagta
     6781 tttcaagtca ggcggaccgc acctatttgt gggaaaaatt ttcaaattat aaaatgtctg
     6841 caaatctgac tgccacttat cggaaattgg aggagatggc caagcaagtg accaatcctt
     6901 cttctcgtta ttatcaagat gaaactgtcg ttcgaacagt cagggattcc atggaatgga
     6961 tgcataaaca tgtctacaat agtgaaaaga gcattgttgg gaactggtgg gattatgaaa
     7021 tcggtacacc tcgtgccatc aacaatacct tgtctctgat gaaagaatac ttctctgatg
     7081 aggaaattaa aaaatataca gatgtgattg aaaaatttgt accagatccc gaacatttcc
     7141 gaaagacgac tgataaccca ttcaaggctc taggtggaaa cttagttgat atgggaaggg
     7201 taaaagtaat agctggttta ctgcgtaagg atgatcaaga aatttcttct accattcgct
     7261 cgattgagca agtgttcaag ttggtagagc aaggtgaagg tttttatcaa gatggatcct
     7321 atatcgacca caccaatgtt gcctatacgg gtgcttatgg gaatgttttg attgatggcc
     7381 tgtctcaact gttgccagtc attcaaaaga ccaagaatcc aatcgataaa gataaaatgc
     7441 aaaccatgta ccactggatt gataaatcgt ttgctccttt gctggtgaat ggagagctga
     7501 tggatatgag tcgtggacgc tcgatcagtc gtgcaaatag cgaggggcac gtggccgcag
     7561 tagaagtact aagagggatt caccgaatag cggatatgtc tgaaggagaa accaaacaac
     7621 gtttgcagag tcttgtgaag accattgttc aatcggatag ttattatgat gtctttaaga
     7681 atttgaagac ttataaggat atcagtttga tgcaatcctt gttaagtgat gcaggagtcg
     7741 caagtgttcc aagaacaagt tacctatctg cctttaacaa gatggataaa acagccatgt
     7801 acaatgcaga gaaagggttt ggatttggct tgtcactctt ttccagtcgt accttgaatt
     7861 acgaacacat gaacaaggaa aataaacgtg gttggtatac gagtgatggg atgttctatc
     7921 tttacaatgg cgatttgagt cactatagcg atggctactg gccaacagtt aatccatata
     7981 agatgcctgg tacaacagag acggatgcta agagagcgga tagcgataca ggtaaagttt
     8041 taccgtctgc tttcgttgga acgagcaaac tagatgatgc caatgcgaca gcaaccatgg
     8101 atttcaccaa ctggaatcaa acattgactg ctcataagag ctggtttatg ctaaaggata
     8161 agattgcctt tttaggaagc aatatccaaa acacttcaac agatactgct gcaactacaa
     8221 tcgaccagag aaaactggaa tcaagtaatc catataaagt ctatgtcaat gataaagaag
     8281 cctcccttac agaacaagaa aaggattatc ctgaaaccca aagtgtcttt ttagaatcgt
     8341 ccgattcgaa aaagaatatt ggttactttt tctttaagaa gagttcaatc agtatgagta
     8401 aggctttgca aaagggagcc tggaaggata tcaatgaagg acagtcagac aaggaagttg
     8461 aaaatgaatt tcttacgatt agtcaggctc ataagcaaaa tggagattct tatggctata
     8521 tgctcattcc taacgtgggt cgtgccacct tcaatcaaat gataaaagag ttagaaagta
     8581 gcctcatcga aaataacgaa acccttcagt ctgtttatga tgccaaacaa ggagtttagg
     8641 gcattgtgaa atatgatgat tctgtctcta ctatttccaa ccaattccaa gttttgaaac
     8701 gtggagtcta taccattcga aaagaagggg atgaatataa gattgcctac tataatcctg
     8761 aaacccagga atcagctcca gatcaggaag tctttaaaaa gctagagcaa gcagctcagc
     8821 cacaagtaca gaattcaaaa gaaaaggaaa aatctgaaga ggaaaagaac cattcggatc
     8881 aaaagaatct ccctcagaca ggagaaggtc agtcaatctt ggcaagtcta gggttcttgc
     8941 tacttggggc gttttattta ttccgtagag gaaagaacaa ctaatttgtt catcatctag
     9001 atgaattaca tgaaactgtg gaaaatagaa aaaatccgaa gaaggccaaa tatcttcgga
     9061 tttttctttt aaaaagggat aagttgatac ttatcctttt ttacttcatt atgtgactat
     9121 agcgttttga aactagaata ggacgtcata actgctaaaa gatttctata aattcatttg
     9181 attttcctaa tcaatttgtt cgtatcctat ttcactccac tatattagtc cgtctcgctc
     9241 cgtgaggtgc gagacaaaac aaccacccgc tatgcgggtg cgcgtcgaag attataccaa
     9301 aaaaactcca aacgcgatac aataaaggtg ttcaatccaa ttgtaaagcg aaaggagaaa
     9361 aaatatggca caaaagactc atagtttatc atacacaaag tggatgtgtt ctatcatatt
     9421 gtgttcaccc ctaagtatag acgaaaagtc atctataatc aatatagaag tagtttaggc
     9481 gaaatatttc atcgcttgtg tagttataaa ggtgttgaaa tgatcgaggg tcacttaatg
     9541 ccagaccatg tacacatgtt agtcagtatt ccactaaggc taagtgtttc aagttttatg
     9601 ggttatttaa aaggcaaaag tgcactcatg atgtttgaca aacacgccaa tctcaagtac
     9661 aagtttggga atcggcattt ttgggcggaa ggttattatg taagtacagt agggcttaat
     9721 aaagccacaa ttaagaaata tagtcaagat taaagaaatt atccagtgga tgatttcttc
     9781 acgagtatga aaatttgaga acgagtaaag catgatatat agtaaaatga aataagaata
     9841 ggacaaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa gcagaggtgt
     9901 actattccag tttcaatcta ctatagcact agataaattg agtgtaaaag aatatgagga
     9961 tccctttagg gatagtggta agtaatacca aagtctctta aagaggcaag tgacgagtca
    10021 agagcaataa ggcttgaaca acgtgaaagc cagcgtcttt aggcgctggc tgatgatttg
    10081 ggcttatagc tctgagataa accacccgtt agacaggtgg ttatgatttt atctgagtgt
    10141 aacatactgt tgggcaatct cgctgatgcg gtcaaagttg ccttgggaag cgagtttatt
    10201 gagttcgcca ccaattccaa cggcgtctgc accagcagcg aaccattgag ggatgttgtt
    10261 tagaccgact cctccggtta ccattacgga aacttgtggg atcggtgcct tgactgcaga
    10321 gatatatgct ggactgagag tactacttgg gaagagtttg atgatttcac taccggcttc
    10381 aagtgcagtc gtgatctctg tgagggtaat acagcctgga atgtacggtg tgctgtagag
    10441 attgcacatt ttcgcagttt cagcatggaa agatggagaa acaacgtaat ttgctccggc
    10501 tagaatggca tctctagcag ttacggcatc aagcacagta cctgcaccga tacaaacact
    10561 cttatcgtcc tgatacaagt ctacaagttc cttgatgatt tgtcctgcat actgattggt
    10621 ataggcgatt tcaatagctt tgataccgcc cttgatacaa gcaatcgagg cttgcagtcc
    10681 ttcttccttt gtatttcccc gaatgacagc gacaattttc tgttttttta gttcaataat
    10741 cgtatctgat ttggtcatgt aattctccta acgaatgata tcttgtgcat ttgccagtaa
    10801 attttcaata ctagttgcgg aagtggagag atggtctcct ggaagagtgc atttgagagt
    10861 tgcgctcgca actgcaaagt caatggtagt ttttagggag gaatgatgga gtagttggta
    10921 aagggcacca gatataaagg catccccgct accaattcgt tgatagactg cagtttttag
    10981 ttggacagac tcttcaaata gttcttctag agcataggct tgatagacat ttttgtcttg
    11041 ctcatcacta gagcggaggg tatggaaaat ggtcttgaaa ccataggctt cttttaaaag
    11101 tcgcatgcga ttttccacct cttctaggct agcactgtct cttggaaaca tctctagatt
    11161 ttggtcatca atcatgagag gatcaatacc gaagcaatag tcagtaaaac gtgcaaactt
    11221 agaaaattca tacttggctt ctaggactga aatcatcttt gttctcagat tgagatccat
    11281 tgaaacgaca attcctcggc gcttggcttc ttccaagagt aggagaagga tcgtacggac
    11341 ctcttgaccg atagctacgg tgattccact aaaatgaaaa tggctaatcc cctgaaagag
    11401 agaatccata tctagcatgt ttggctgaat ctggctgata ctcgtatgct tacgatcgta
    11461 gaaaacttca ctttgacgac aaccaaagcc gttctccaaa tagtagaggc cgattcgatc
    11521 gccaagccga cagattgaac tggtatcgat ttggtgctgt ttcaagaatg tgagaaaacg
    11581 atctccaatc tcgttggcag ggagtgcggt aaagactttc gttgagatac ccagggcttg
    11641 caaattacaa gcgatgttaa tttctgatcc gccaaaataa gtcgaactgg caacatgatc
    11701 gccgatactg gtggcatcta atggtgagat tcgaattaat ggttcgccaa acagtaatat
    11761 tttcgtcatc cattagttcc tttctggttt agaatagctt tacttggcta atacttcttt
    11821 caaataagca gcgacttcat catcttggca gttttcaaag aagtattctt ggaagtattt
    11881 gccagagatg gtgtctttga ggaagtcttg gtcgattgtt ttaaggatgg tcatcaaatc
    11941 attgtgggtg atttgtttca cctcgtttaa gatacgagcg ttgcgttgtt cagggattac
    12001 acgttctctt gggtagccac cgcccatttc ttcagcaaat aagcgttcaa acatcaattt
    12061 gagggtcagt tctgcccccc agccaaatcc tttggcataa gggatagaca aggcgttacc
    12121 accattgatt tgagaataaa ggtaagcgtc agttgggtcc actgctagac cacagacaac
    12181 accagggaag ctgtttaaag caagcatagc ccctacaccc gtaccacagc cggtaacaac
    12241 aaagtcaact gcctttgtat ttaaaaggat ggcagccatt agtccgttct gcacataagt
    12301 taattgactt tctccttctt ctccacgcat accatagtta aataattggt agcctttttt
    12361 atctgtcgct tcttttagac tatcgtaaat aatgtgattc ttgctagctt gactattttc
    12421 attgattaat gcgattttca taagatcttc tttctatttt tactcaggtt gttttccgat
    12481 gtaggctaag ataccgccat ctacatatag gatgtggcca ttgacaaaat tgctggcatc
    12541 actagcgaga aagacagcag ggcccatcaa atcttcagga tttccccaac gtgcagcagg
    12601 tgtttttgca atgatgaact ggtcaaatgg gtggcgagaa ccatcttctt gcaattcacg
    12661 aagaggtgct gtttgaggag tggcaatata acccggtcca attccgttac attggatatt
    12721 ggctccaccg tattcagacg caatgttgcg ggtcaacatt ttcaagcccc ctttagcagc
    12781 agcataagcg ctaactgttt cacgtcccag ttcgctcatc atcgaacaaa tattgataat
    12841 ctttccatgc cctttcttta tcatagaagg aataactgcc tttgaaacga taaatggtgc
    12901 gtttaagtca atatcgatga ccttacggaa atcagcggcg ctcatttcgc acattggaac
    12961 tcggcggata ataccagcgt tattaacgag gatgtcaatg acaccaacct cttgttcgat
    13021 ttgcttgacc atggcttgga taccgtcctc gtctgtcacg tcacagacat atccatgggc
    13081 ttcgatgcca acttcacgat aagcttcaat ccctttattg accagatctt gattgatatc
    13141 gttaaagaca atagtagcgc cggcttcagc gtaggatttg gcaatagcaa atccaattcc
    13201 ataagaagcg ccggtgatga gagcaatttt tccttttaaa gaaaactgct caattgagaa
    13261 tgatgtattt gtcatttgat acttcctttc aaatttgtta agacttctct ataaccaaat
    13321 tatattctag taaaccggtt ttgtcaataa ataaatattt ttatttataa aaagatttgt
    13381 tttcctaaaa aaacggaaga ttttgaaatt tctgtctatt aaaatggtta aaaagataat
    13441 gaaagcgcta atattttttt aaaaaaacta ttgacaagat aaaaataaag gattacaatg
    13501 gggaatataa agtaaaccgg taaacctaaa caagaaagga gaaaagatga aaattgtact
    13561 tgtagggcat ggacattttg ctacagggat ttatagttct ttacaattga ttgcaggcaa
    13621 tcaagaaaat gtggaggcga ttgactttgt ggaaggaatg tcagcagatg aactcaagca
    13681 aaaaatctta cttgcaattt caaatgaaga agaagtttta atcctaagtg atctcttggg
    13741 aggatcgcca ttcaaggttt cttctaccat aatgggagaa aatccagcca aaacaatgaa
    13801 tgttctctcg ggtttgaact tagccatgtt aatggaagca gtctttgcta gaatggctca
    13861 tagctttgat gaagttgtta ataaatcagt agtggcggcc cagggcggag tcgtaaatgg
    13921 taaagaattg ttttcaacgg atgcagagga agaggaagaa gatttcgaat cgggtattta
    13981 aagggtaaaa gaatgataaa aaaggttacg attgaaaaaa taaaatcgcc tgagcgcttc
    14041 ttagaagtac cacttctgac gaaagaagaa gtcggccagg caatcgataa ggttattcgg
    14101 cagttagaac tcaaccttga ctatttcaag gaagatttcc cgacaccagc tacctttgat
    14161 aatgtctatc caatcatgga taacacggaa tggaccaatg gtttctggac aggagaactg
    14221 tggttggctt atgaatacag tcaacaggat gcatttaaaa acatcgctca taaaaatgtt
    14281 ctttctttcc tggatcgtgt caataagaga gtagaattgg ctcaccatga tctcggcttc
    14341 ttgtacacac cgtcttgtat ggctgaatat aagataaatg aagatggaga ggctagagaa
    14401 gcagccttga aagctgcaga taagttgatt gaacgctatc aagaaaaagg tggttttatt
    14461 caagcttggg gagacttggg caagaaagag cattaccgtt tgattatcga ttgcttgctc
    14521 aatatccaac tcttattctt tgcttatcaa gaaacaggcg atcaaaaata ctacgatatt
    14581 gcagaaagcc atttctatgc ttcagctaat aatgtaatcc gtgatgacgc ttcgtccttc
    14641 cacaccttct attttgatcc tgagacaggt caacccttta aaggtgtaac gagacaaggg
    14701 tatagtgatg attcatgctg ggcacgtggt cagtcatggg gagtctatgg tattcctttg
    14761 acttatcgtc acttaaaaga cgagtcctgc tttgacttgt ttaagggtat gaccaattat
    14821 ttcttgaatc gtctgccaaa agatcatgtg tcctattggg atttgatttt taatgatggt
    14881 agtgatcaat cacgagattc ttcagcaaca gctatcgccg tctgtgggat tcatgaaatg
    14941 ctaaaacatc tcccagaggt ggatgctgac aaagatattt ataaacatgc tatgcatgcc
    15001 atgcttcgtt ccttgatcga acattatgca aatgatcaat ttacccctgg tgggacaagt
    15061 ctcctccacg gtgtgtactc atggcattca ggtaaaggag tggatgaagg caatatctgg
    15121 ggtgactact attacctaga agcccttatc cgtttctaca aagactggaa cctatattgg
    15181 tagaggagaa atatgacaat gccaaatatt attatgaccc gtatcgatga acggttgatt
    15241 catggacaag gacaactttg ggtaaaatac ctaggttgta atacggtcat tgttgccaat
    15301 gacgaagtaa gcacggacaa gatgcaacaa actctgatga aaacagttgt gccagactca
    15361 gttgccatgc gtttcttccc tttgcaaaag gtgattgata tcattcacaa ggctaatcct
    15421 gctcaaacga tctttatcgt tgtaaaggat gtgaaggatg ctttaacctt ggtagaaggt
    15481 ggtgtcccta tcaaagaaat caatattggg aacattcaca atgctactgg taaagagcaa
    15541 gtgacacgct ccatcttcct gggtgaagag gacaaggtgg ccctcaagga attgagccaa
    15601 actcatcaag taacatttaa tacgaaaaca actccaacag gaaatgatgg agctgttcaa
    15661 gtcaacatta tggactatat ttaaaagagg agatcgttat gtcgattaat gtatttcaag
    15721 cgattttaat tggattatgg acagctttct gttttagtgg aatgctgtta ggaatttaca
    15781 ccaatagatg tattgttctg tcatttggtg tcggaattat tctaggtgat ctgcctactg
    15841 ctcttgcaat gggagctatt ggtgaattgg cttatatggg attcggtgtt ggtgctggag
    15901 gtactgttcc accaaaccca atcggacctg gtatctttgg taccttgatg gctatcacta
    15961 gtgctggtaa agtcagtcca gaagcggctc ttgccctctc tactccgatt gctgtggcga
    16021 ttcaattctt acaaactttc gcctacactg tacgtgctgg tgcgcctgaa acagcgatga
    16081 agcacttgaa aaaccataat ttgaagaaat ttaagttcac tctaaatgca acaatttggt
    16141 tgtttgcctt tattggattt accttgggtt gcttgggtgc cctttcaatg gataccttgt
    16201 tgaaactcgt agactacatt ccaccggtat tgcttacagg tttgacagtt gctggtaaaa
    16261 tgctcccagc tatcggtttt gcgatgatct tgtcagtgat ggctaagaaa gagttgattc
    16321 cctttgtctt gttgggatat gtttgtgcag cttatctaaa catcccaaca attggtattg
    16381 caattgtagg tactatcttt gctttgattg aattttataa caagccaaaa acagcggatc
    16441 atgtggtaga ggaggaagca cacgatgact ggatctaaca aattaacaaa acgtgattat
    16501 cttaaaacgt ctttgcgggc attcttttgt caaaatggat ttaactatag taactatcaa
    16561 gggttgggat atgccaatgt gatgtatcct gctttgaaaa aacactatgg agaggatcag
    16621 gaaggtttct accaagcctt ggaagaaaac tgtgaattct ataataccaa cccacacttc
    16681 ctgcctttta ttaccagctt gcatcttgta atgttggaaa atggccgtcc ggcaaaagaa
    16741 acacgtagta tcaagatggc cttgatggga ccattggcag gtattgggga ttctctttct
    16801 caattctgtt tagctccatt gttctcaacc atcgcagctt cgtttgctca agaaggcttg
    16861 gttgtcggcc caatcttgtt cttccttgcg atgaatacga ttttaacagc gattaaattg
    16921 tcaactggtt tgtatggata caaactagga acaactgtga ttgataaact aagcgaacag
    16981 atggcaacga tttctcgtat tgccaatatt atcggtgtaa ccgtaattgc tggtttggca
    17041 gcgacatctg ttaagattat ggtgccgatt acctttgctg caggggaagt taaagcagac
    17101 gctaaacaaa gtatcgtaag tattcaggga atgcttgata aggttgctcc agctcttcta
    17161 ccagccctat ttacactttt agtttactac ttgatcaaag aaaagaaatg gacaacatat
    17221 aaactcgtta ttttaacagt tatcatcgga attatcggaa gctggcttaa gattatagct
    17281 taatggatac aacattgttt tatggaatag tgattgtctt ggcagtgagt ccccttttac
    17341 tgtcaagctt tcattctatt cgtcagcaaa agttgcttcg caaacagatg gagcaacgac
    17401 aagagtattt agcttcttta acatctggtg atgaagtgtt gttattgtct ggaattcatg
    17461 gaaaaatcat ttctatcaaa gatgacttgg tctccttgca gattgcaaaa ggtgtggtca
    17521 tctatgtaga aaaggaaagt gtaatgggaa agacaaaaga actgcttttt aagtagttct
    17581 ttttctggta atagataatg aaagaagaaa gaagacaatt ttttgaaaga atcgatggaa
    17641 accaatgtcg tgattatatc ttgtcccact gttcaaaaga ctatgagaaa gtcaagagtt
    17701 cccttgaacg cttgatggac aatcgtttta tgtttgatag tccttgggat atggagcctt
    17761 gttcaaaaat ccatcaaatc cagccgatgg tatgggatca agtatttgaa gatgatccag
    17821 aatgggctta tatgctcaat cgacaagaat atctcttgca gtttatgata gggtatgtgg
    17881 tagaaggaga taaggactat attcaaaagt gcaagttctt tctatttgat tggattgagc
    17941 aggtgagaga attttctcct caatccttga tgactagaac cttggatacg ggtattcgtt
    18001 cctttacttg gttgaaacta ctcttgctcc tcttgaaatt tgacttgcta gaggagaaag
    18061 aactagagaa aattttggtc agtctagaaa agcagattga ctttatgaaa agctactatc
    18121 gcgccaagta cacccttagt aactggggga ttttacaaac aattccgatg cttgctatct
    18181 atcatttctt tgcagataag atggacctag aagaagctta ccattttgct tcagaggagt
    18241 tgaaacagca aattgagaca cagattttag gagatggaag ccagtttgaa cagtcgattc
    18301 tctatcatgt agaggtttat aaagccttgc tggatttgtg tctcttgctt ccagacttgc
    18361 aagatagtta ccaagagttg ctggaaaaga tggcgaccta tattcaaatg atgacaggct
    18421 tagatggacg aactttggct tttggtgata gcgattctac agaaacgaca gaaattttga
    18481 gcctgtctgc tgtggttttg aacaaggaag accttcttaa cggtctggat gttaaagtcg
    18541 atttgcttag cctcttgttc ctggggcgag aaaaggtcaa gcgactgcag gaatttgaaa
    18601 agagagcttg gcagcctaag tccatgatct ttgaagactc tggacatgtc tgcattaagg
    18661 atgaacatcg ttatctattt ttcaaaaatg gtccgctagg aagtgcccat agccatagtg
    18721 acgagaatag tttttgctta cagtatcaag gccaacctat tttcatagat gctgggcgtt
    18781 attcttatcg ggagatatat gaacgttatc tcttaaagag tgcttggagt cattcgacct
    18841 gcattgtaga tgggaaagct ccggaaagaa tcacgggatc ctgggaatat gaatactatc
    18901 ctcactccct gttttgtcac cataaagaaa gggagggaat gcattatatt gaaggggctt
    18961 attggtcagc agaacctgat ttgccttatc ttcacaagag aaaaatcctc atgttggtag
    19021 aggatgtctg gctcttggta gatgacatca ggtgtcaagg tcagcatgag gcgttgactc
    19081 agtttatcct tgacaaggat gtgacctatc aagatgggaa aatcaatcag ttgagactat
    19141 ggagtgaagt tgattttgat ttggaagata ccatcatttc tcctaaatac aatgagcttg
    19201 aaagaagtag caaactcacc aagcgccaat tctttgagaa tcagatgctg gattatacca
    19261 tcattgcgca tgagagtttt gaaatcatcc gtcattctgt ctaccagaca gatgatcgtg
    19321 aagtggaaaa tgctctggct tttgaagtga aaaatgacga aacagacaag ctgattctgt
    19381 tattaagcga ggatattggt gtaggtgaaa aattgtgcct cgttgacgga acaaaaatgc
    19441 gtggaaaatg tctagtatat gataaaataa atgagagaat gattcgcttg cagtgctaga
    19501 aataggcatt ttgaatagtg aatatgttat aataagtatt agtaggaggt gttttagatt
    19561 ggagaagaaa ctgaccataa aagacattgc ggaaatggct cagacctcga aaacaaccgt
    19621 gtcattttac ctaaacggga aatatgaaaa aatgtcccaa gagacacgtg aaaagattga
    19681 aaaagttatt catgaaacaa attacaaacc gagcattgtt gcgcgtagct taaactccaa
    19741 acgaacaaaa ttaatcggtg ttttgatcgg tgatattacc aacagtttct caaaccaaat
    19801 tgttaaggga attgaggata tcgccagcca gagtggctac caggtaatga taggaaatag
    19861 taattacagc caagagagtg aggaccggta tattgaaagc atgcttctct tgggagtaga
    19921 cggctttatt attcagccga cctctaattt ccgaaaatat tctcgtatta tcgatgagaa
    19981 aaagaagaaa atggtctttt ttgatagtca gctctatgaa caccggacta gctgggttaa
    20041 aaccaataac tatgatgccg tttatgacat gacccagtcc tgtatcgaaa aaggttatga
    20101 gcattttctc ttgattacag cggatacgag tcgtttgagt actcggattg agcgggcaag
    20161 tggttttgtg gatgctttaa cagatgctaa tatgcgtcac gccagtctaa ccattgaaga
    20221 taagcatacg aatttggaac aaattaaaga atttttacaa aaagaaatcg atcccgatga
    20281 aaaaactctg gtatttatcc ctaactgttg ggccctacct ctagtcttta ccgttatcaa
    20341 agagttgaat tataacttgc cgcaagttgg gttgattggt tttgacaata cggagtggac
    20401 ttgcttttct tctccaagtg tttcgacgct ggttcagccc tcctttgagg aaggacaaca
    20461 ggctacaaag attttgattg accagattga aggccgcaat caagaagaaa ggcaacaagt
    20521 cttggattgt agtgtgaatt ggaaagagtc gactttctaa aatgaaggaa aatgacttgc
    20581 aatctctgtt aagaaataaa ataatcccac ctagcttgtt ctaggtggga ttatttgcct
    20641 atgaaatgag aaattatggg agcaagctcc taaatcaact gtttttgatc tacttcttta
    20701 actacttgat aaaagttata gaagtaggcc aaacttgaaa tgatggttac gactaggaat
    20761 attgaaaatt tccattggac agggttggtt aaaagttgtg gaaaggatat gaggagaaag
    20821 aagagggctg cgttgaggac aggtatccgt tttgattgta ttttctcaag tcctttattg
    20881 agcgcaggaa gaaagaggag taggagtagt aaaactgtat gagaaacagc tcctgaaata
    20941 agggcgaaga aaaggaaaat actgataaaa acatgaatgt tcagtagtct agctagtgat
    21001 ttcataaggc acctcctaat cctggtcttt tttagctctt gcaatacgaa gtgagtcgac
    21061 aatatgtatc atcactccga aaaagaaagc tcccagtata gttttaaaaa tatgttttgt
    21121 atttagaaga gaactgataa aatttggatt ttcacttgtt agggtatcaa tgagtggaat
    21181 tataaaaaat atcactgttc cataaatcga acctgctttc agaccaggat aacgtaactg
    21241 tttcttttct tttttcatga gtttcctcct aatcctcatc ttgatttttc ttagtttttg
    21301 caatgcgacg ggagatgagg aactgtatgc tcgctccgaa gaaaatagaa ccgagaatac
    21361 ttgatacacc atttcttata gtgagaagag aatgaaaata gtcctgacct tcatctatga
    21421 gtatactgag aagaggagtt ataaaaaaca tccatagacc aaagaacaaa cctgctttaa
    21481 gacctgggta gtgtagttgc ttgctttctt tctcattcag catatctggt tcaatgactg
    21541 tgatgcctgt ttttttcatt tggtaggtga catagccagc agcgatgagg gcaattacta
    21601 aaatcagagg aggatagatt agagccactt cttgagggta tttataggcc agaaggagtg
    21661 gaataagatt tccgaaaatc atcagataaa agaggacgat aaagacttgg ttcccaatac
    21721 tatcggcctc acgccgtttg tattcgtcaa ggggaccaga aataccgtat gtgcgtttga
    21781 tcagtttttc agtgaaggtt tcttttttca tgagtttgct ccttttttaa aaatcttcct
    21841 cccaaaagag actgttgagg tcagtttgga ggctgcgggc gagattgaga cagagttcca
    21901 aggttggatt gtacttgtcg ttttcaatca tattgatagt ctgtctcgag acaccgatat
    21961 ccttggcgag ttcgagctgg gaaataccca attccttgcg aaattctttc acacgattca
    22021 tctggtctcc tttctgattt atgtcgtata tatttgacta tattatagtc ttttaaacat
    22081 aaagtgtcaa gtatttttga catatttttt gaagaaatag tagtctcctt gtcctatttg
    22141 tctgacaagt gcaagctggt cggatttgtg gtaaaataga taagatatga caaaagaatt
    22201 tcatcatgta acggtcttac tccacgaaac gattgatatg cttgacgtaa aacctgacgg
    22261 tatctacgtt gatgcgactt tgggtggagc aggccatagc gagtatttat taagtaaatt
    22321 aagtgaaaaa ggccatctct atgcctttga ccaggatcag aatgccattg acaatgcgca
    22381 aaaacgcttg gcaccttaca ttgagaaggg aatggtgacc tttatcaagg ataacttccg
    22441 tcatttacag gcacgtttgc gcgaagctgg tgttcaggaa attgatggaa tttgttatga
    22501 cttgggagtg tctagtcctc aattggacca gcgtgagcgt ggtttttctt ataaaaagga
    22561 tgcgccactg gacatgcgga tgaatcagga tgctagtctg acagcctatg aagtggttaa
    22621 tcattatgac tatcatgatt tggttcgtat tttcttcaaa tacggtgagg ataaattctc
    22681 taaacagatt gcgcgtaaga ttgagcaagc gcgtgaagtg aagccgattg agacaacgac
    22741 tgagttagca gagattatca agttggtcaa acctgccaag gaactcaaga agaagggtca
    22801 tcctgctaag cagattttcc aggctattcg aattgaagtc aatgatgaac tgggagcggc
    22861 agatgagtcc atccagcagg ctatggatat gttggctctg gatggtagaa tttcagtgat
    22921 tacctttcat tccttagaag accgcttgac caagcaattg ttcaaggaag cttcaacagt
    22981 tgaagttcca aaaggcttgc ctttcatccc agatgatctc aagcccaaga tggaattggt
    23041 gtcccgtaag ccaatcttgc caagtgcgga agagttagaa gccaataacc gctcgcactc
    23101 agccaagttg cgcgtggcca gaaaaattca caagtaagag ggaaaaagat ggcagaaaaa
    23161 atggaaaaaa caggtcaaat actacagatg caacttaaac ggttttcgcg tgtggaaaaa
    23221 gctttttact tttccattgc tgtaaccact cttattgtag ccattagtat tatttttatg
    23281 cagaccaagc tcttgcaagt gcagaatgat ttgacaaaaa tcaatgcgca gatagaggaa
    23341 aagaaaaccg aattggacga tgccaagcaa gaggtcaatg aactattacg tgcagaacgt
    23401 ttgaaagaaa ttgccaattc acacgatttg caattaaaca atgaaaatat tagaatagcg
    23461 gagtaagata tgaagtggac aaaaagagta atccgttatg cgaccaaaaa tcggaaatcg
    23521 ccggctgaaa acagacgcag agttggaaaa agtctgagtt tattatctgt ctttgttttt
    23581 gccatttttt tagtcaattt tgcggtcatt attgggacag gcactcgctt tggaacagat
    23641 ttagcgaagg aagctaagaa ggttcatcaa accacccgta cagttcctgc caaacgtggg
    23701 actatttatg accgaaatgg agtcccgatt gctgaggatg caacctccta taatgtctat
    23761 gcggtcattg atgagaacta taagtcagca acgggtaaga ttctttacgt agaaaaaaca
    23821 caatttaaca aggttgcaga ggtctttcat aagtatctgg acatggaaga atcctatgta
    23881 agagagcaac tctcgcaacc taatctcaag caagtttcct ttggagcaaa gggaaatggg
    23941 attacctatg ccaatatgat gtctatcaaa aaagaattgg aagctgcaga ggtcaagggg
    24001 attgatttta caaccagtcc caatcgtagt tacccaaatg gacaatttgc ttctagtttt
    24061 atcggtctag ctcagctcca tgaaaatgaa gatggaagca agagcttgct gggaacctct
    24121 ggaatggaga gttccttgaa cagtattctt gcagggacag acggcattat tacctatgaa
    24181 aaggatcgtc tgggtaatat tgtacccgga acagaacaag tttcccaacg aacgatggat
    24241 ggtaaggatg tttatacaac catttccagc cccctccagt cctttatgga aacccagatg
    24301 gatgcttttc aagagaaggt aaaaggaaag tacatgacag cgactttggt cagtgctaaa
    24361 acaggggaaa ttctggcaac aacgcaacga ccgacctttg atgcagatac aaaagaaggc
    24421 attacagagg actttgtttg gcgtgatatc ctttaccaaa gtaactatga gccaggttcc
    24481 actatgaaag tgatgatgtt ggctgctgct attgataata atacctttcc aggaggagaa
    24541 gtctttaata gtagtgagtt aaaaattgca gatgccacga ttcgagattg ggacgttaat
    24601 gaaggattga ctggtggcag aatgatgact ttttctcaag gttttgcaca ctcaagtaac
    24661 gttgggatga ccctccttga gcaaaagatg ggagatgcta cctggcttga ttatcttaat
    24721 cgttttaagt ttggagttcc gacccgtttc ggtttgacgg atgagtatgc tggtcagctt
    24781 cctgcggata atattgtcaa cattgcgcaa agctcatttg gacaagggat ttcagtgacc
    24841 cagacacaaa tgattcgtgc ctttacagct attgctaatg acggtgtcat gctggagcct
    24901 aaatttatta gtgccattta tgatccaaat gatcaaactg ctcggaaatc tcaaaaagaa
    24961 attgtgggaa atcctgtttc taaagatgca gctagtctaa ctcggactaa catggttttg
    25021 gtagggacgg atccggttta tggaaccatg tataaccaca gcacaggcaa gccaactgta
    25081 actgttcctg ggcaaaatgt agccctcaag tctggtacgg ctcagattgc tgacgagaaa
    25141 aatggtggtt atctagtcgg gttaaccgac tatattttct cggctgtatc gatgagtccg
    25201 gctgaaaatc ctgattttat cttgtatgtg acggtccaac aacctgaaca ttattcaggt
    25261 attcagttgg gagaatttgc caatcctatc ttggagcggg cttcagctat gaaagactct
    25321 ctcaatcttc aaacaacagc taaagctttg gagcaagtaa gtcaacaaag tccttatcct
    25381 atgcctagtg tcaaggatat ttcacctggt gatttagcag aagaattgcg tcgcaatctt
    25441 gtacaaccca tcgttgtggg aacaggaacg aagattaaaa acagttctgc tgaagaaggg
    25501 aagaatcttg ccccgaatca gcaagtcctt atcttatctg ataaagcaga ggaggttcca
    25561 gatatgtatg gttggacaaa ggagactgct gagacccttg ctaagtggct caatatagaa
    25621 cttgaatttc aaggctcggg ctctactgtg cagaagcaag atgttcgtgc taacacagct
    25681 atcaaggaca ttaaaaaaat tacattaact ttaggagact aatatgttta tttccatcag
    25741 tgctggaatt gtgacatttt tactaacttt agtaggaatt ccggccttta tccaatttta
    25801 tagaaaggcg caaattacag gccagcagat gcatgaggat gtcaaacagc atcaggcaaa
    25861 agctgggact cctacaatgg gaggtttggt tttcttgttt acttctgttt tggttgcttt
    25921 ctttttcgcc ctatttagta gccaattcag caataatgtg ggaatgattt tgttcatctt
    25981 ggtcttgtat ggcttggtcg gatttttaga tgactttctc aaggtctttc gtaaaatcaa
    26041 tgaggggctt aatcctaagc aaaaattagc tcttcagctt ctaggtggag ttatcttcta
    26101 tcttttctat gagcgcggtg gcgatatgct ttctgtcttt ggttatcaag tgcatctagg
    26161 gattttctat attgtcttcg ctcttttctg gctagtcggt ttttcaaacg cagtaaactt
    26221 gacagacggt gttgacggtt tagctagtat ttccgttgtg attagtttgt ctgcctatgg
    26281 agttattgcc tatgtgcaag gtcagatgga tattcttcta gtgattctgg ccatgattgg
    26341 tggtttgctc agtttcttca tctttaacca taagcctgct aagatcttta tgggtgatgt
    26401 gggaagtttg gctttaggtg gaatgctggc agctatctct atggctctcc accaagaatg
    26461 gactctcttg attatcggaa ttgtgtatgt ttttgaaaca acttctgtta tgatgcaagt
    26521 cagttatttc aaactgacag gtggtaaacg tattttccgt atgacgcctg tacatcacca
    26581 ttttgagctt gggggattgt ctggtaaagg aaatccttgg agcgagtgga aggttgactt
    26641 cttcttttgg ggagttgggc ttctagcaag tctcctgacc ctagcaattt tatatttgat
    26701 gtaagaatgg caccctgatg ttttagggtg tttttgtgtt taaatacaca atgaaaatca
    26761 aagaacaaac tagaaagcta actttaggct gctcaaaaca tagtagattg aaactaaaat
    26821 agtacacctc tacttctaaa atattgttag aaatcgattt gactgtcctg aacgatttat
    26881 cctgttctta tttcatttta ctatacagtt ttgagatggt tgatagaact gacgaagtca
    26941 gctcaaaaca cggttttgag gttgtagata aggagaagct gatgtggttt gaagaggttt
    27001 tcgaagagtg taaaaaaata ttggtcagtt aaagtcaaaa ctattgacct tttctgacta
    27061 atgtagtata ttatgaacgt aaggtaaatg aaagccttac tagaacactt atttaaagta
    27121 aaatagaaag aggtgagcct atgtttcatc tagaaatctt cagaagtaaa gatagtctac
    27181 tcctgcttga aaaagaaaaa ccagaaatag tacgtagagt agcggtttag ctagtctaaa
    27241 ttttttaaaa caaaggtcaa agatagtcaa tatcagtaat cataactaag taaacaaaaa
    27301 gaggtaaaga atatgaataa caactttaat aattttaata acatggatga tttatttaac
    27361 caattgatgg gtggtatgcg aggatacagt tctgaaaatc gccgttactt aattaatgga
    27421 cgcgaagtca cacctgagga atttgctcac tatcgtacga ctggtcaatt accaggaaat
    27481 gcagaaactg atgtgcaaat gccacaacag gcatcaggta tgaaacaaga cggtgtcctt
    27541 gcaaaactag gtcgaaactt gacagcagaa gcgcgtgagg gcaagttgga tcctgttatc
    27601 ggacgaaaca aggaaattca agaaacatct gaaatcctct cacgccgcac caagaacaat
    27661 cctgttttgg tcggagatgc aggtgttggt aagacagcag ttgtcgaagg tctagcgcaa
    27721 gccattgtga acggagatgt tcctgctgct atcaagaaca aggaaattat ttctattgat
    27781 atctcaggtc ttgaggctgg tactcaatac cgtggtagct ttgaagaaaa tgtccaaaac
    27841 ttagtcaatg aagtgaaaga agcagggaat attatcctct tctttgatga aattcaccaa
    27901 attcttggtg ctggtagcac ttgtggagac agtggttcta aagggcttgc ggatattctc
    27961 aagccagctc tctctcgtgg agaattgaca gtgattgggg caacaactca agacgaatac
    28021 cgtaacacca tcttgaagaa tgctgctctt gctcgtcgtt tcaacgaagt gaaggtcaat
    28081 gctccttcag cagagaatac ttttaaaatt cttcaaggca ttcgtgacct ctatcaacaa
    28141 caccacaatg tcatcttgcc agacgaagtc ttgaaagcag cggtggatta ttctgttcaa
    28201 tacattcctc aacgtagctt gccagataag gctattgacc ttgtcgatgt aacggctgct
    28261 cacttggcgg ctcaacatcc agtaacagat gtgcatgctg ttgaacgaga aatcgaaacg
    28321 gaaaaagaca agcaagaaaa agcagttgaa gcagaagatt ttgaagcagc tctaaactat
    28381 aaaacacgca ttgcagaatt ggaaaggaaa atcgaaaacc acacagaaga tatgaaagtg
    28441 actgcaagtg tcaacgatgt ggctgaatct gtggaacgaa tgacaggtat cccagtatcg
    28501 caaatgggag cttcagatat cgaacgtttg aaagatatgg ctcatcgctt gcaagacaag
    28561 gtgattggtc aagataaggc cgtagaagct gtagctcgtg ctatccgtcg taaccgtgct
    28621 ggttttgatg aaggaaatcg cccaatcggc agcttcctct ttgtagggtc tactggggtt
    28681 ggtaagacgg agcttgctaa gcaattggca ctcgatatgt ttggaaccca ggatgcgatt
    28741 atccgtttgg atatgtctga atacagtgac cgcacagctg tttctaagct aattggtaca
    28801 acagcaggct atgtgggtta tgatgacaat agcaatacct taacagaacg tgttcgtcgc
    28861 aatccatact ctatcattct cttggatgaa attgaaaagg ctgatcctca agttattacc
    28921 cttctcctcc aagttctaga tgatggtcgt ttgacagatg gtcaaggaaa tacagtaaac
    28981 ttcaagaaca ctgtcattat tgcgacctca aatgctggat ttggctatga agccaacttg
    29041 acagaagatg cggataaacc agaattgatg gaccgtttga aacccttctt ccgtccagaa
    29101 ttcctcaacc gctttaatgc agtcatcgag ttttcacact tgactaagga agacctttct
    29161 aagattgtag atttgatgtt ggctgaagtt aaccaaacct tggctaagaa agacattgac
    29221 ttggtagtca gtcaagtggc taaagattat atcacagaag aaggttacga cgaagtcatg
    29281 ggggttcgtc ctctccgtcg cgtggttgaa caagaaattc gtgataaggt gacagacttc
    29341 cacttagatc atttagatgc taaacatctg gaagcagata tggaagatgg cgttttggtt
    29401 attcgtgaga aagcctaaga tagaattttg aggataaaaa agaaggagcc agctgaaaaa
    29461 aactggttcc ttttgaggta cgacgggcat gtcgtatagt agaagtgtac tattctagtt
    29521 tcaatatact atagtagctc agaagtcgat acttaaacgt gctatatcaa aaccagtcct
    29581 tgaaaaacgc ggactggttt cgtgtttgga ttattacctt gaacgacatg cgttaaaagt
    29641 tagttgaacc gccgtatgcc gaatggtacg tacggtggtg tgagaggggc tagagattat
    29701 cccctactcg attttaaatc acatgacgtt caaaggcatc atctgaaatc ccttgttcca
    29761 agatgagttt tgcccattct ttagcagaga agaggctgtg gtccttgtag tttccgcaag
    29821 attcgatggt tgtccctggg acatcttccc aagtagtagt ttcagcgatt tccttgagcg
    29881 aatccttgat aacagctgcg attttagcac tggtgtgacg tccccacata atcatgtgga
    29941 agcctgtgcg gcaaccaaat ggtgaacagt caatcatgcc gtcaatgcgg gtacggatga
    30001 gtttggctaa gaggtgctcg atagtgtgaa ggccggcagt agggatagag tcttcgtttg
    30061 gttgcaccaa gcgaatatca taattggaga tgatgtctcc tttgggtcct gtttcttccc
    30121 caatcaagcg aacatagggt gctttgacaa tggtgtggtc aagttcaaaa ctttcgacaa
    30181 taacttcttt tgacatggta aatcctttca gttttcttct ctcattatat cataaaggtt
    30241 gctcctgaga cagagagaaa acctctccga ggctggagag gttgaaatct ttacttacga
    30301 tataagcggt cgtattggta gtatgggtca aaggttacgt tgatacccag tttacgaagg
    30361 acattcttgt cttcatcagt caagatgatg gttgagtggg cttcgcttcc tttgaggttg
    30421 ccgagttctt ccatagcgcg ggcagcatca ggattttctg tagctgtgat agcaagtgca
    30481 atcaggattt catttgaatg aaggcgtgga ttgcggctac cgagatgatc gattttaaga
    30541 ccttggattg gcttaacaac ttcaggctcg attagtttta cttctttagc gatgtcagct
    30601 gattttttga tggcgttgat caaggcagcg gctgtaggac caaagagttc tgagttctta
    30661 ccagtgacga tttccccatt tggcaattca aaggctaggg ctggtccacc agtttcttct
    30721 gctttttggc gcgcaacgac agcaacctta cggtctgcag gtgtgatacc gaggtcgttc
    30781 atgagcaact caattttctt gacggcagct tcgccaactt tttcagcttt gaagtcaaga
    30841 actgtttgat agtaacggcg gatgatttct tgtttagaag cttcgacagc agcctcgtca
    30901 tctgtaatag cgaaaccaac catgttgaca cccatatctg tcggtgaagc gtatggtgat
    30961 tttccgagaa tacgttccaa catgcgcttg agcactggga agatttcgat atcacggttg
    31021 tagttgacag tggtttctcc ataggtttga agatggaagg ggtcaatcat gttgacatca
    31081 tcaaggtcag ctgtggcagc ttcataagcc aagttaactg gatgatgaag gggaagattc
    31141 caaacaggga aggtttcaaa tttagcgtag ccagacttga tgccattgat ttggtcgtgg
    31201 tacatattgg acatacacgt tgccaatttt ccagaaccgg gtccaggagc ggttacgaca
    31261 atcaagttgc gactgatttt gatgtagtcg tttttgccca tgccttctgg ggaaatgatg
    31321 tgatccatat ccgtcggata tcctttgatt ggataatgaa gataagaatc aattccgttt
    31381 ttctcaagtt ggttgcggaa ggcatctgca gcgggttgac cagtgtattg tgtaatgaca
    31441 acggaaccaa caaaaatccc taattcattg aatttgtcaa tcaaacgaag aacttcttgg
    31501 tcataagaaa tgcctaagtc gccacgtgct ttggaatgtt caatgttgct agcattaatg
    31561 gcaatcacaa cctcaacctg ctctttcaat tcttgcaaga gcttgatttt gttgtcaggt
    31621 tcataaccag gaaggacacg agcagcgtgg aaatcttcta acattttacc gccaaactct
    31681 aagtagagct tgccgtcaaa ttggttaatg cgctccaaaa tatggtcgcg ttgtaaattc
    31741 aaatattgtt cagaactaaa agcttgtttt ttcatttttt acctctggac tctattataa
    31801 taaaaaattg gaagttagga aactacggag ctaaaaaaga aattaaaaag attaagcaaa
    31861 cgcttgcaca aaattttaaa aagtgctatc atatactata gattattaaa ataatgaggt
    31921 aagaagatgc aagaaaaatg gtggcataat gccgtagtct atcaagtcta tccaaagagt
    31981 tttatggata gtaatggaga tggagttggt gatttgccag gtattaccag taagttggac
    32041 tatctagcta agctaggaat cacagcgatt tggctttctc ccgtttatga cagccctatg
    32101 gatgataatg gctacgatat tgctgattat caagcgattg cggctatttt tggaaccatg
    32161 gaggacatgg atgaactgat tgcagaagct aagaagcgtg atatccgtat catcatggac
    32221 ttggtggtca atcatacctc ggatgagcat gcctggtttg tagaggcctg tgaaaatcct
    32281 aatagccctg agcgagacta ctatatctgg cgcgatgaac ccaatgacct agattctatc
    32341 tttagtgggt ctgcttggga atacgatgaa aagtcaggtc aatactatct ccactttttc
    32401 agcaagaaac agccggatct caactgggaa aatgaaaaac ttcgccagaa aatttatgag
    32461 atgatgaact tctggattga taagggtatt ggtggtttcc gtatggatgt tattgacatg
    32521 attggcaaaa ttcctgacga gaaggtagtc aataatggtc ctatgctcca tccctatctc
    32581 aaggaaatga atcaggtgac ctttggagat aaggatctct tgacagtagg ggagacttgg
    32641 ggagcaacgc cagagattgc caaactctac tctgatccaa aggggcaaga attgtctatg
    32701 gtcttccagt ttgaacatat cggtcttcag tatcaggaag gtcagcctaa atggcactat
    32761 caaaaagagc tgaatatcgc taagttaaaa gaaatcttca acaaatggca gacagagtta
    32821 ggagttgagg acggctggaa ttccctcttc tggaacaacc atgacctccc tcgtattgtc
    32881 tcaatctggg gaaatgacca agaataccgc gaaaaatctg ccaaagcctt tgcaatcttg
    32941 cttcatctta tgagaggaac tccttatatc taccaaggtg aggagattgg gatgaccaac
    33001 tatccgtttg aaacactgga tcaagtagaa gatattgaat ctctcaacta tgcgcgtgag
    33061 gctcttgaaa aaggtgttcc gatgcaagaa atcatggaca gtatccgtgt tattggacgt
    33121 gacaatgccc gtacccctat gcaatgggac gagagcaaaa acgctggttt ctcaacaggt
    33181 caaccttggt tggcagttaa tccaaattac gagatgatca acgtccaaga agcgctggca
    33241 aatccagatt ctattttcta tacctatcag aaactggtcc aaattcgcaa ggagaatagt
    33301 tggctaattc gagctgactt tgaattgctt gatacggctg ataaggtctt tgcttatata
    33361 cgtaaggatg gcgaccgtcg cttcctagtt gtggctaact tgtccaatga agagcaagac
    33421 ttgacagtag aaggaaaagt caaatctgtc ttgattgaaa acaccctagc tcaagaagtc
    33481 tttgaaaaac aaatcttagt tccatgggat gctttctgtg tggaattact ataaatattt
    33541 tttgcagaaa aatttaaaaa tgaaatcgta taaaaacaag ggaggactgt ataaaagaca
    33601 gaaatccttt gttttttata accaaagttt ataaaccttc attcttgaaa ttcaattaac
    33661 tttacaaatt cccactatta aggagaaaga agatgaacat aaagaagcgt gtccttggtg
    33721 caggtctgac ttttgcatct gctttgcttt tagctgcttg cggccaatca ggttcagata
    33781 caaaaactta ctcatcaacc tttagtggaa atccaactac atttaactat ctattagact
    33841 attacgctga taatatagtc aattgaaaca agagcaggac aaaagagcct cgtaaaaggt
    33901 attgcaacaa cttggtaata cctttttgag gtgctttttg atatgagccc atgttttctc
    33961 aataggattg tactcaggtg agtagggagg aagaggtaaa agtttatgcc caaactcttc
    34021 gc
//
LOCUS       DAVKAO010000016        33681 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKAO010000016 DAVKAO010000000
VERSION     DAVKAO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33681
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.016"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..234
                     /locus_tag="VWX16_000787"
     CDS             <1..234
                     /locus_tag="VWX16_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9158173.1"
                     /translation="NSKPQADDKKPSTPNNLSKDKQSSNQASTNENKKQGPATNKPKK
                     SLPSTGSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(454..1434)
                     /gene="dusB"
                     /locus_tag="VWX16_000788"
     CDS             complement(454..1434)
                     /gene="dusB"
                     /locus_tag="VWX16_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9158174.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1421..2293)
                     /gene="hslO"
                     /locus_tag="VWX16_000789"
     CDS             complement(1421..2293)
                     /gene="hslO"
                     /locus_tag="VWX16_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9158175.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2458..3885
                     /locus_tag="VWX16_000790"
     CDS             2458..3885
                     /locus_tag="VWX16_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9158176.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4044..5552
                     /gene="glpK"
                     /locus_tag="VWX16_000791"
     CDS             4044..5552
                     /gene="glpK"
                     /locus_tag="VWX16_000791"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9158177.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5594..7420
                     /gene="glpO"
                     /locus_tag="VWX16_000792"
     CDS             5594..7420
                     /gene="glpO"
                     /locus_tag="VWX16_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9158178.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7490..8194
                     /locus_tag="VWX16_000793"
     CDS             7490..8194
                     /locus_tag="VWX16_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9158179.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8385..8588
                     /locus_tag="VWX16_000794"
     CDS             8385..8588
                     /locus_tag="VWX16_000794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158180.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8675..9283)
                     /locus_tag="VWX16_000795"
     CDS             complement(8675..9283)
                     /locus_tag="VWX16_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158181.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSDYTGYVKTVTTYVNF"
     gene            10314..11591
                     /gene="ltrA"
                     /locus_tag="VWX16_000796"
     CDS             10314..11591
                     /gene="ltrA"
                     /locus_tag="VWX16_000796"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU9158182.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKFLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGIIINGQRHKTLVGTPQGGNLSPLLSNVMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSASRFIEKRLGLKVNMTKAKITRPGELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     RIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            12049..12760
                     /locus_tag="VWX16_000797"
                     /pseudo
     CDS             12049..12760
                     /locus_tag="VWX16_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12955..13205
                     /locus_tag="VWX16_000798"
                     /pseudo
     CDS             12955..13205
                     /locus_tag="VWX16_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
     gene            13519..13650
                     /locus_tag="VWX16_000799"
     CDS             13519..13650
                     /locus_tag="VWX16_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9158183.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            13671..15221
                     /gene="dltA"
                     /locus_tag="VWX16_000800"
     CDS             13671..15221
                     /gene="dltA"
                     /locus_tag="VWX16_000800"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9158184.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            15218..16462
                     /gene="dltB"
                     /locus_tag="VWX16_000801"
     CDS             15218..16462
                     /gene="dltB"
                     /locus_tag="VWX16_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9158185.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            16476..16715
                     /gene="dltC"
                     /locus_tag="VWX16_000802"
     CDS             16476..16715
                     /gene="dltC"
                     /locus_tag="VWX16_000802"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9158186.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            16708..17976
                     /gene="dltD"
                     /locus_tag="VWX16_000803"
     CDS             16708..17976
                     /gene="dltD"
                     /locus_tag="VWX16_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9158187.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMRHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            18131..18571
                     /gene="adcR"
                     /locus_tag="VWX16_000804"
     CDS             18131..18571
                     /gene="adcR"
                     /locus_tag="VWX16_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9158188.1"
                     /translation="MRQLAKDIDAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            18571..19275
                     /locus_tag="VWX16_000805"
     CDS             18571..19275
                     /locus_tag="VWX16_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9158189.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            19268..20074
                     /locus_tag="VWX16_000806"
     CDS             19268..20074
                     /locus_tag="VWX16_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9158190.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTISTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            20084..21589
                     /gene="adcA"
                     /locus_tag="VWX16_000807"
     CDS             20084..21589
                     /gene="adcA"
                     /locus_tag="VWX16_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9158191.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQSLDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(22781..23536)
                     /locus_tag="VWX16_000808"
     CDS             complement(22781..23536)
                     /locus_tag="VWX16_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9158192.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGE"
     gene            23712..25115
                     /locus_tag="VWX16_000809"
     CDS             23712..25115
                     /locus_tag="VWX16_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9158193.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDLSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLLSQGKINEDTELK
                     DIMTNIADTKYYYPQLS"
     gene            25144..26436
                     /locus_tag="VWX16_000810"
     CDS             25144..26436
                     /locus_tag="VWX16_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9158194.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVHTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
     gene            26523..27452
                     /locus_tag="VWX16_000811"
     CDS             26523..27452
                     /locus_tag="VWX16_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9158195.1"
                     /translation="MAQKIMSLQNRKNQKRRFIFLFLLPTLICFFLFYFYSVVTIFLT
                     SFAKWDYTNLNTPEFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAV
                     SVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFK
                     DSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYI
                     TIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYAR
                     ANTIGVIQILGGMLIIVCINILFRERKRLKGEK"
     gene            27455..28294
                     /locus_tag="VWX16_000812"
     CDS             27455..28294
                     /locus_tag="VWX16_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9158196.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            28291..29610
                     /locus_tag="VWX16_000813"
     CDS             28291..29610
                     /locus_tag="VWX16_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9158197.1"
                     /translation="MKHTLETINSRIQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEVYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            29616..31487
                     /locus_tag="VWX16_000814"
     CDS             29616..31487
                     /locus_tag="VWX16_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEU9158198.1"
                     /translation="MKIENKNVRRNFFWGEGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIKLSSTHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKYLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKKMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTAVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDSQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            31509..>33681
                     /locus_tag="VWX16_000815"
     CDS             31509..>33681
                     /locus_tag="VWX16_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9158199.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYKKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLGTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSRFDEFFYGDELMNAGIKVSLSNLALCVPEY"
BASE COUNT        10315 a         5840 c         7216 g        10310 t
ORIGIN      
        1 aatagcaagc cacaagcaga tgataagaag ccatcaactc caaataattt aagcaaggac
       61 aagcaatctt ctaaccaagc ttcaacaaac gaaaacaaga agcaaggtcc agcaacaaat
      121 aaaccgaaga agtcattgcc atcaactgga tctatttcaa atctagcact tgaaattgca
      181 ggtcttctta ccttggcggg ggcaaccatt cttgctaaga aaagaatgaa atagtgattg
      241 attcattcaa aaatgtttgt aaaaagtatt cggcttagaa agaaaagagt ctgggataaa
      301 aagattttga ttttaggaat tctttattat aaatttttaa aatcgataga ttagacaaaa
      361 aagcgaacaa gacagaattc tgagtgtcag ataactgatt ttgtttgctt tttatattta
      421 atattatact tttgtcctag gctctttttt gcattatact ttttctaatt gcaagagggc
      481 ttcaatctct gctagggtgc tagcttgcga aatggctcca cggagtttgg cagcgccaga
      541 tgttccacgg agatagtgag gagcgaggcc gcggaattca cgaactgcga cgttttctcc
      601 tttgaggtta atcaatcgtt tcaagtgttc gtaggcgatc ttcatcttgt cttcaaaggt
      661 caaatcaggt aggatttctc ctgtttcaaa gtaatggttg atttggttga agaggtaagg
      721 atttcccatg gcagctcggc caatcatgac tgcgtcagca ccaacttctt cgatgcgttg
      781 cttggcttct tggacagtac ggatatcacc gttggcgatg aatggaatct tggttagagc
      841 ttgggcaacc ttgtaaaggg tctcaaggtc tgcgtggcca gtatacattt gttcacgggt
      901 acggccatgc atggcgaggg cagaaacacc tgcagcctca gcagcgaggg cattttctac
      961 tgccagagat gggtccgccc agccggtacg cattttgaca gtaagtggga tatcaaggac
     1021 agactggacc ttgttgatga tagagtagat cttgtcagga tccttgagcc acatagctcc
     1081 agcttcgttc ttcacgattt tgttgacagg gcagcccatg ttgatatcga cgatatcggt
     1141 cttggtgttt tcttggatga attctgctgc gcgtgctagg ctgtcttcat cgctaccaaa
     1201 aagttggata gagacagggt tttcgccctc atcgatatga agcatatgca gggttttttc
     1261 gttgttgtat tggattccct tgtcagagac catttccatt acaacgagtc cagctccgag
     1321 ctcttttgcg atggtacgaa aggctgagtt ggtcacgcca gccataggcg ctaaaacggt
     1381 acgattggga atctcaatat tgccaatcat aaaaggtgta ttaagatttg tcacgaatga
     1441 gttcctccag gtccttttca tcaaagttgt aagtagtttg gcagaattga caagtgattt
     1501 ctgccccgtg gtcttcctct ttcatttcct gtaagtctga gcttggaagg ctggcaagag
     1561 cgttcataaa gcgttcatgg ctacagtcac attggaaacg gatttcttct tcagaaagac
     1621 gcttgtaggc ttcgtccccg tagatagcct tgaggagggc ttcgatatgg tcgtcgcttt
     1681 cgagaagagt agagatagct ggcatttctt ggatgcgttt ttcaaagcga gcaatctctt
     1741 ctttcttggc tcctggcaag acttgaacta ggaaaccacc tgcaaccttg accttgtctt
     1801 cctcgtccaa aaggacattg aggccgaccg ctgaaggcgt ttgttggctt tcagtaaggt
     1861 aaaaggcaag gtcttcaccg atttctccag agatgagggg agttatagag ttgtaaggat
     1921 ttccagtacc gtagtctgtg ataacgagga attgaccatt tccaacaaaa ggtccgacta
     1981 ggacttcacc agtcgcagtc tttttgatgt caacaccagg attttgaaca tagcctttga
     2041 cgttcccctt ggtatcagcg acggtgataa tagcacctag agagctagag cccaacacct
     2101 taactgtaag tttggtattt cctttttcat tggctgcgag aatctggctg gcgataagag
     2161 ttcgaccaag cgctacagtt gagctagctt gggtttgatg tttttcttga gcagtgcgga
     2221 cggtttccgt gctatcaagg acaaaagcac gaaaggctcc gctttctgat atagttttaa
     2281 taattttatc catagctact attttagcat aaaaatgccc aaagggggag ccgtgtgttt
     2341 actgattttc aggataatgg accaggaaat cagcatgaaa ataaaaagag aaacagatta
     2401 ttttagcatt tgtaagattt atgctatgct taaggtagaa aatgaaaggg gtaacaaatg
     2461 tatttaggag atttgatgga gaaagccgag tgtggtcaat tttcaatcct ttcctttcta
     2521 ttacaagagt ctcagacgac cgtcaaggct gtaatggaag aaacaggatt ttcaaaagca
     2581 accctaacca aatatgtcac cctgctcaat gacaaggctt tggatagtgg cttagagctg
     2641 gctattcact cagaagatga aaatctgcgt ctgtctatcg gtgcagctac caaggggaga
     2701 gatattcgga gcttgttttt ggagagtgct gttaaatacc agattttggt ttatcttctc
     2761 taccaccaac agtttttagc ccatcagctg gctcaagaat tggtgattag cgaggctacg
     2821 cttggtcgtc acttggctgg tttaaatcag attttgtcag aatttgattt atccatccaa
     2881 aatggacgtt ggcgaggtcc agagcatcag attcgctatt tctatttctg tcttttccga
     2941 aaggtctggt cgagtcagga atgggaaggt cacatgcaga aaccagagag aaaacaggag
     3001 attgccaatt tagaggaaat ctgcggtgca agtttgtctg tggggcagaa attggacttg
     3061 gttctctggg ctcacatcag tcaacaacgt cttcgggtca atgcttgtca gtttcaagtc
     3121 atagaagaga aaatgcgagg gtattttgac aatattttct accttcgttt gctgagaaag
     3181 gttccgtcct tttttgctgg gcaacatatt ccactaggag ttgaggatgg tgagatgatg
     3241 atattcttct cttttctcct atctcatcgc attcttcctc ttcatactat ggagtatatt
     3301 cttggttttg gagggcagtt ggcagattta ctgacgcaat tgattcaaga aatgaagaag
     3361 gaggaactat tgggggatta tacagaggac catgtcacct atgaactcag tcagctttgt
     3421 gctcaagtct atctctataa gggctatatt ttacaggatc gctacaagta ccagttagag
     3481 aatcgtcatc catatttact gatggaacat gattttaaag agacagcaga ggagattttt
     3541 catgctctac ctgcttttca acaggggaca gatttagata agaagattct ctgggaatgg
     3601 ctccagttaa tcgaatatat ggctgaaaac ggtggccagc atatgcggat tggtctggat
     3661 ttgacatctg gttttcttgt cttttcaagg atggcagcca ttttgaaacg gtatttggaa
     3721 tacaatcgtt ttattaccat tgaagcttat gaccctagtc ggcattatga tttgctggtt
     3781 accaataacc cgattcataa gaaggaacag acaccagtct attatttaaa aaacgacttg
     3841 gatatggaag atttggcagg gattcgtcag ttattattca cttaaaaggc ttggttaatc
     3901 taggtctttt ttgtgaaatt cacacaatct cctcacattt ttttaaaaat taaaaaaagt
     3961 tgataaacaa gaaagcgctt tattttgtat actagtaagt gtaaagagga aacacctcaa
     4021 gatctttatc aggaggacag tacatgtcac aagaaaaata catcatggcc attgaccagg
     4081 gaactacaag ttctcgtgcc atcattttca acaaaaaagg ggaaaaggtt agctcgagtc
     4141 aaaaagagtt tacccagatt ttccctcagg caggttgggt tgagcacaat gccaatgaaa
     4201 tttggaactc tgttcagtca gttattgcgg gtgctttcat cgaaagtggt gtcaagccaa
     4261 atcaaatcga ggcaatcggg attaccaacc aacgtgaaac aacggttgtc tgggataaga
     4321 aaacaggact tcctatctac aatgctatcg tttggcagtc acgccagaca gcacctttgg
     4381 ctgagcaact aaaaagccaa ggttatgtgg aaaaattcca tgaaaagact ggtttgatta
     4441 ttgatgctta cttctctgct accaaggttc gttggatttt ggatcatgta gaaggtgctc
     4501 aagagcgagc agaaaaaggg gaattgctct ttggtactat cgatacttgg ttggtttgga
     4561 aattgactga cggtgcggct cacgtgactg actactcaaa tgcagctcgt accatgcttt
     4621 ataacattaa agaactcaaa tgggatgatg agattttgga aatccttaac attccgaagg
     4681 ctatacttcc agaagttcgt tctaactccg aaatctacgg caagacagct ccattccatt
     4741 tctacggtgg agaggtgcca atctcaggta tggctgggga ccaacaagca gccctctttg
     4801 gacagttggc ttttgagcca ggtatggtta agaatactta tggaacaggc tctttcatca
     4861 tcatgaatac tggggaagag atgcagttgt ctgaaaacaa cctcttgaca accattggtt
     4921 acggaatcaa cggtaaggtt tattatgcct tggaaggttc tatcttcatc gcaggaagtg
     4981 ctattcagtg gcttcgtgac ggtcttcgca tggttgaaaa ttcaccagaa tctgaaaaat
     5041 acgctcgtga ttctcacaac aacgatgaag tttatgtcgt tccagccttt acaggtctag
     5101 gcgctccata ctggaaccaa aatgctcgtg gttccgtctt tggtttgact cgtggaacaa
     5161 gcaaagaaga ctttatcaag gcgactttgc aatctattgc ttatcaagtg cgtgatatca
     5221 tcgacaccat gcaagtggat actcagaccg ccattcaagt actgaaggtg gatggtggtg
     5281 cagccatgaa caacttcctc atgcagttcc aggcggatat tttaggcatt gacattgcac
     5341 gtgctaaaaa cctggaaaca acagctctag gagcggcctt cctagcaggt ttgtcagtag
     5401 ggtactggaa agacttggac gagttgaaac tcttgaacga gacaggagaa ctctttgagc
     5461 catctatgaa cgaatctcgc aaggaacaac tctacaaggg ctggaagaag gctgtgaaag
     5521 caactcaagt ctttgcggaa gtagacgact aatactggca gaataaagcg atttatttag
     5581 aaagtgtgta aatatggaat tttcaaagaa aacacgtgaa ttgtcaatta aaaaaatgca
     5641 ggaacgcacc ctggacctct tgattatcgg tggaggaatc acaggagctg gtgtagcctt
     5701 gcaggcggca gctagcggtc ttgagactgg tttgattgaa atgcaagact ttgcagaagg
     5761 aacatctagt cgttcaacaa aattggttca cggaggtctt cgctacctca aacaatttga
     5821 cgtagaagtg gtctcagata cggtttctga acgtgcagtg gttcaacaaa tcgctccaca
     5881 cattccaaaa ccagatccaa tgctcttacc agtttacgat gaagatggag caacctttag
     5941 cctcttccgt cttaaagtag ccatggactt gtacgacctt ttagcaggtg tgagcaacac
     6001 accaactgcg aacaaggttt tgagcaagga tcaagtcttg gaacgccagc caaacttgaa
     6061 gaaggaaggc ttggtaggag gtggagtgta tcttgacttc cgtaacaacg atgcgcgtct
     6121 cgtgattgaa aacatcaaac gtgccaacca agacggtgcc ctcattgcca accacgtgaa
     6181 ggcagaaggc ttcctctttg acgaaagtgg caagattaca ggtgttgtag ctcgtgatct
     6241 cttgacagac caagtatttg aaatcaaggc ccgtctggtt attaatacaa caggtccttg
     6301 gagtgataaa gtacgtaatt tgtctaataa gggaacgcaa ttctcacaaa tgcgcccaac
     6361 taagggagtt cacttggtag tggattcaag caaaatcaag gtttcacagc cagtttactt
     6421 tgacacaggt ttgggtgacg gtcgtatggt ctttgttctc ccacgtgaaa acaagactta
     6481 ctttggtaca actgatacag actacacagg tgatttggag catccaaaag taactcaaga
     6541 agatgtagat tatctacttg gcattgtcaa caaccgcttt ccagaatcca acatcaccat
     6601 tgatgatatc gaaagcagct gggcaggtct tcgtccattg attgcaggga acagtgcctc
     6661 tgactataat ggtggaaata acggtaccat cagtgatgaa agctttgaca acttgattgc
     6721 gactgttgaa tcttatctct ccaaagaaaa aacacgtgaa gatgttgagt ctgctgtcag
     6781 caagcttgaa agtagcacat ctgagaaaca tttggatcca tctgcagttt ctcgtgggtc
     6841 tagcttggac cgtgatgaca atggtctttt gactcttgct ggcggtaaaa tcacagacta
     6901 ccgtaagatg gctgaaggag ctatggagcg cgtggttgac atcctcaaag cagaatttga
     6961 ccgtagcttt aaattgatca attctaaaac ttaccctgtt tcaggtggag aattgaaccc
     7021 agcaaatgtg gattcagaaa tcgaagcctt tgcgcaactt ggagtatcac gtggtttgga
     7081 tagcaaggaa gctcactatc tggcaaatct ttacggttca aatgcaccga aagtctttgc
     7141 acttgctcac agcttggaac aagcgccagg actcagcttg gcagatactt tgtcccttca
     7201 ctatgcaatg cgcaatgagc tagctcttag cccagttgac ttccttcttc gtcgtaccaa
     7261 ccatatgctc tttatgcgtg atagcttgga tagcatcgtt gagccagttt tggatgaaat
     7321 gggacgattc tatgactgga cagaagaaga aaaagcaact taccgtgctg atgtcgaagc
     7381 agctctcgct aacaacgatt tagcagaatt aaaaaattaa gaaaaaataa aagaggtgga
     7441 gggcagcatt ccttgtcgct cgtcccttct ttttaatgga gacagaaaga tgatgaatga
     7501 attatttgga gaatttctag ggactttaat cctaattctt ctaggaaatg gtgttgttgc
     7561 aggtgtggtt cttcctaaaa ccaagagcaa tagctcaggt tggattgtga ttactatggg
     7621 ttgggggatt gcagttgcgg ttgcagtctt tgtatctggc aagctcagtc cagctcattt
     7681 aaacccagct gtgaccatcg gtgtggcctt aaaaggtggt ttgccttggg cttccgtttt
     7741 gccttatatc ttagcccagt tcgcaggggc catgctgggt cagattttgg tttggttgca
     7801 attcaaacct cactatgagg cagaagaaaa tgcaggcaat atcctggcaa ccttcagtac
     7861 tggaccagcc atcaaggata ctgtatcaaa cttgattagc gaaatccttg gaacctttgt
     7921 tttggtgttg acaatctttg ctttgggtct ttacgatttt caggcaggta tcggaacctt
     7981 tgcagtggga actttgattg tcggtatcgg tctatcacta ggtgggacaa caggttatgc
     8041 cttgaaccca gctcgtgacc ttggacctcg tatcatgcac agcatcttgc caattccaaa
     8101 caagggagac ggagactggt cttacgcttg gattcctgtt gtaggccctg ttatcggagc
     8161 agccttggcc gtgcttgtat tctcactttt ctaatctaga aaacaattat gttgatagag
     8221 cttgggcaag agcccaattt cagcaaaaaa tgaagtaaat cttctcataa taaaacgcat
     8281 catatcaagc acgaaaattc cacgaggtca actacagtca gaaagctgaa caacaagcca
     8341 aaacgcccaa aaaaggcggc aaaaagcaag cacctgcaag caacgtgccg aaatggtcaa
     8401 atcctgatta tgtcaacgaa ttagacccaa aaatcgttga tatgctagta gaatttcaca
     8461 agtcacaagg cactttggaa actcccgagg cgcaagcaga aatcgcccaa aaacgtgaag
     8521 aaatcgagca aaggagagct gagcttgagg ataaaaaaca agagcttttg aaccgcttga
     8581 acaaatagag tttcgcaagt attatgctta caaattactt gagcaattaa ctaaaatata
     8641 aaccatgcct ttatatctag gcagggttta tattttagaa attcacgtag gttgttacgg
     8701 tttttacata cccagtatag tctgagtttc tatagtattc agtgataaac ttccattttc
     8761 tttgagcaac atggatataa gtacttgtta tgtagtatgg atatgggctt tgtgaatcca
     8821 agtaagactg ataagcttgt ataccaaaat atgctccacc aattattgca ccccatggac
     8881 cccccaataa agcacctatc ctaccaatca tataactgat tccagcacca gtcatgaagt
     8941 tagcgaatgt gttagcttgt ttattcccat gtattgtgtt gacgtaattc caaacattag
     9001 gatcgtatga tctaaaagat atatttaggt caatttcatt cttttgataa gccatataaa
     9061 atgccccatt gatatagacg ccgtcagcac gtcgttcaat agtgtctaca cttccatctg
     9121 gattgacaac ctcaagaact tcatcgctta aaatatttac ttgcgtatct ccgaaccgca
     9181 ctgatgagcc attctcaaac tgagcctcac cagatacaac tttagagttt gccgataagc
     9241 tatcatcagc aaaaacaaca agcgacgggg aaatgctaga catacagaaa acagacataa
     9301 ctagcaaaca catgcattta agcatcttag acataacgga aactcctttg tatttttgat
     9361 ttttttcaac ttttattata caataaaacc aaataaaaag aaagcggtaa caatatgctt
     9421 aatgcgaaaa ttttttatat atttttatgt ttgatcgtta tcgaaactac aggcttgttg
     9481 ttgttgaaaa gaggtctcga aatgggttat ttagacacag aagctattat cctcgcagtt
     9541 ttttcatttg ctttttacaa cctatgttca ttcgcttggg tctgctctac aataaaaaac
     9601 aataaaaaat aaatagacgt attttcaaaa aaaaatgcat atttatctta gcagaacgac
     9661 gatttaaatc gtcgtttttt tgtagtacga cgggcatgtc gtatatctga ggtgtaagtc
     9721 ctcggtgggc acctgctacc ggtgaaccca atagcgattc tcaagcctga ctatcgtgag
     9781 gtagcaggga gaggaaggga tagcgaaatc gtggctctac gaacaggaac gtgatagtaa
     9841 ggcgtatata gcggataagg gggcctcaaa ctctaaagtc caaaaaggta gtcgtaacct
     9901 atatgcgtaa atcacgagag taattgaatt cggactaagg tttgtgtgaa aaagataaat
     9961 ctttctagcg tctaaagact ctgcgtcaga tttcctattt tcactgtaac cttttaacgt
    10021 cctcatatct tgtataaacg aggaaagatg tacgacttat cccgtgaggt ctcatgagcg
    10081 ctgaaagcgt agtaacaacg aatcatgaga agtcagccga acccatagta gtgaggaaac
    10141 ttctgtaatg gaagtggagc gaaggggtga acaactaatt gaagtaatcc tacttcactt
    10201 gtgtctgtaa aacgagaggt ctgatagaac tggagtctgt cacgtattga ctagatgaag
    10261 gctcaccaat ataagatgtc cctcaggcac caaaacaaga aaggaatacg cacatgtcaa
    10321 aattgctaga taagatatta tcacgcgaaa atatgctgga agcctacaat caagtaaaat
    10381 ccaataaagg ctcagctggg attgatggaa tgactatcga agagatggat aattatctca
    10441 gacaaaactg gcgcttgact aaggaactga taaaacagag aaaatataag cctcaaccag
    10501 ttcttagagt tgagatacct aaaccagacg gaggcatccg tcaactagga attccaacag
    10561 ttatggatag aatgattcaa caggccattg tccaagttat tagtcccatt tgtgaacccc
    10621 atttctcaga tacgagttat ggcttcagac caaataggtc atgtgaaaaa gccatcatga
    10681 agttcttaga gtacttaaat gacggctatg agtggatagt ggatatagac ctagagaaat
    10741 ttttcgatac ggttcctcaa gatagattga tgtccttggt acataacatt atcgaagacg
    10801 gagatacgga atccctgatt cgtaagtatc ttcattcagg tattatcatt aatggtcagc
    10861 gtcataaaac gctagttggc acaccacagg gaggaaattt atctcctctc ttatccaatg
    10921 tcatgcttaa tgaattggac aaggaattag aaaagagggg acttcgattt gtgcgctacg
    10981 cagatgattg tgtgattacg gtcggaagcg aggcagccgc taagcgtgtg atgtattcag
    11041 ccagtcgttt tattgagaag cgactaggtt tgaaagtaaa tatgaccaag gctaagatta
    11101 ccagaccagg agagctgaaa tacttaggtt ttgggttctg gaaatcatca gatggttgga
    11161 aaagtcgtcc ccatcaagat agtgttcgga gatttaagct taaattgaag aaactaacac
    11221 agaggaaatg gagtatagac ttaacaagac gtattgagca actgaatttg tctattcgag
    11281 gatggataaa ctatttctcg ctgggaaata tgaagcgcat agtcgctagc atagatgagc
    11341 gcttgcgtac tcgcctacga gtgattatct ggaagcaatg gaagaagaaa tcgagacgac
    11401 tatggggatt gcttaagtta ggagttccta aatggatagc agataaggta tctggctggg
    11461 gcgaccatta tcaattagta gctcagaagt cggtacttaa acgtgctata tcaaaaccag
    11521 tccttgaaaa acgtggactg gtttcgtgtt tggattatta ccttgaacga catgcgttaa
    11581 aagttagttg aaccgccgta tgccgaacgg catgtacggt ggtgtgagag gggctagaga
    11641 ttatccccta ctcgattaac tcccctgaaa tttattttaa ttatgcaaat ttcacgtatt
    11701 tttgatgctg agacgacgat ctgggaactt ttcagatatt tttttgacta tctaaaatct
    11761 atcattagaa aagcttagag cgccaaagga tttgagcgtt tttctgattt ttaagacttt
    11821 tccccagtct ctttttcgat tgaagatgta attattctac taactaacta acttcttagt
    11881 actagccaac aacgataatc ataattcctc ctaaaattag gaataataaa ggcaatagtt
    11941 tttgtttttt catgtaaaaa acctcacttt tgttttctgc tattttatgc taaaatatta
    12001 aaaatcaaat ttaattccaa agtttgtaac taaagggggg agcgctacat gtctaattca
    12061 tttgtcaagt tgttagtctc tcaattattt gcaaatttag cagatatttt ctttagagta
    12121 acaatcattg ctaacatata cattatttca aaatcagtaa ttgccacatc actagttcct
    12181 atcttaatag gaatatcctc ttttgttgcg agtcttttag ttccgttggt tactaaaagg
    12241 ttagcgctaa atagggtttt atctttatct caatttggaa agactatatt attggcgata
    12301 ctggtaggaa tgtttaccgt aatgcaatcc gtagcgcctt tggtgaccta tctatttgtt
    12361 gttgcaattt ccatactaga tggttttgca gcacccgttt cctatgctat tgtgccacgc
    12421 tatgcgaccg atttgggtaa ggctaattca gccttatcaa tgactggtga agctgctcaa
    12481 ttgataggtt ggggattagg tggactcttg tttgcaacaa ttggtctgtt acctaccacg
    12541 tttatcattt tagtcttgta tatcatttct agctttctga tgttatttct tcctaacgct
    12601 gaagtggagg tgttagagtc agaaactaat cttgaaattt tgctcaaagg ttggaagtta
    12661 gttgctagaa atcctagatt aagacttttt gtatcagcaa atttattgga aattttttca
    12721 aatacgattt gggtttcttc cattatactt gtttttgtaa cggagttatt aaataaaacg
    12781 gaaagttact ggggatattc taatacagca tactctattg gtattataat tagtggctta
    12841 attgctttta ggctatctga aaagttcctt gctgctaaat gggaaagtat tctttttcct
    12901 cttgtagcta tggcaatagt gacactgact attctatatt ttccaaacgc acagatgttt
    12961 ttattatttt cagctttagt tggtatgtta tcgcaactaa aagaagttcc tgaaagtgta
    13021 tttcttcagg aaacagtaga ggaaaatcat ttagttaatg tctattccgt tcttgaagtg
    13081 atttctacat tagcattttc agtatttgtt ttgctaatga gctatattac tgattttggc
    13141 tatcagccat ttgtctgatg atagaagcta ttttaattta tatcagacga gattatttta
    13201 aatgagagag tggttaaaaa gtctttaaaa atcagaaaag cgcatagtat caggtgttga
    13261 aaaaacttga tattgtgtgt tttattatgg gaagatttac ttcattgtct cctgaaatgg
    13321 agttttgaag cagaatcact ctatttgaaa atatacatag aaaaaccgca taatccttaa
    13381 aataagctga aatctattgg aaacatacgg taaacaattg aaaagatact atataaagtg
    13441 gtacaatggt agaagaataa gctagtaaga atcactctta ttttagcgaa aaattacaga
    13501 aatgaatggt ttttcttgat gaaacagaga aaagaattgt acctctttct tggtaggaca
    13561 gccttgtatt ttcttatctt tctagggctg ctttacttct ttagctatct tggtcagggt
    13621 caaggaagct ttatctataa tgaattttaa cttgaaggag aatccctatt gtgtcaaata
    13681 aaccaatagc agatatgatt gaaaccattg agcattttgc tcagacacag cctagctatc
    13741 ctgtctataa tgttttgggg caggaacaca cttatggcga tttaaaggct gattcggata
    13801 gtttggctgc agtcattgac caactaggct tgcctgagaa gtctcctgtg gttgtttttg
    13861 gtggccaaga atatgaaatg ttggcaacct ttgtagcgct gactaagtca ggtcatgcct
    13921 acattccaat tgatagccat tcggccttgg agcgagtttc agctatttta gaagtagcag
    13981 agccaagctt gattattgcc atttcagcct ttcccttgga gcaggtttct acaccaatga
    14041 taaatctagc tcaggttcaa gaagcctttg cccaagggaa caactatgaa atcacgcatc
    14101 cagtcaaggg agatgataat tactacatta tctttacttc tggtacgact ggtaagccta
    14161 agggagtgca gatttcacat gataatctcc tcagctttac aaactggatg attacggata
    14221 aggaatttgc gacaccgagt cgtccgcaaa tgctggcaca accaccttat tcttttgact
    14281 tgtctgtcat gtattgggca ccgaccttgg cacttggtgg tacgcttttc actcttcctt
    14341 cagtcatcac tcaggacttt aagcaactct ttgcggctat cttttcattg ccaatcgcta
    14401 tctggacatc aacaccatcc tttgcagata tggccatgtt gtctgaatac ttcaacagtg
    14461 agaaaatgcc tggaatcacg catttctact ttgatggtga agaattgacg gtcaaaacag
    14521 ctcaaaaact gcgcgagcgt ttcccaaatg cccgtatcat caatgcttac ggcccaacag
    14581 aagcgacagt agctctgtca gcagttgccg tgacagacga gatgttagcg actctcaaac
    14641 gcctaccaat cggctatacc aaggctgatt ctccaacctt tatcattgac gaggaaggaa
    14701 ataaactgcc aaatggcgaa cagggagaaa tcattgtttc tgggccagct gtttcaaaag
    14761 gttatatgaa caatcctgaa aaaacagcag aagccttttt tgagtttgaa ggtctgccag
    14821 cctatcacac aggcgatgtg ggaaccatga cagatgaggg cttgcttctc tacggcggac
    14881 gcatggactt ccagattaag tttaacggtt accgcattga gttagaagat gtctctcaaa
    14941 acctcaacaa gtctcgcttt atcgaatctg ctgtcgcagt accgcgctat aacaaggacc
    15001 acaaggtaca aaatctattg gcttatgtca tcttaaaaga cggtgttcgt gagcagtttg
    15061 agcgagatat cgatattacc aaggccatca aggaagacct gacagacatc atgatgtcct
    15121 atatgatgcc atctaaattc ctttaccgag acagtttgcc actaactcca aatggaaaga
    15181 ttgacatcaa aggattgatt aacgaggtga ataagagatg atggagtttt ttcaacagct
    15241 tcctcattta gagccatatg gcaatcctca gtattttgtt tatgtgattg ctgcaacctt
    15301 gcccatcttt ataggtctct ttttcaagaa acgctttgcc tggtatgaag tgttggtaag
    15361 tctcttcttt attgtcacca tgttggtggg tggaaagacc aatcaactag ctgccttggg
    15421 tatttacctt tgctgggaaa tattgctcct gcttttctac aagcattatc gaaaaagcaa
    15481 ggatggcaag tgggtcttct acttagttag ttttctgtcc ctacttccga ttatctttgt
    15541 caaggtgcaa ccagctatca atggaacgca gtctttgctt gggttcttgg gaatttctta
    15601 cctgaccttt cgttcggttg ggattatcat cgagctgaga gatggagtga ttaaggattt
    15661 taccctctgg gaattcctcc gtttccttct cttcatgcca actttctcga gtggtccaat
    15721 cgatcgtttt aagcgattta atgaaaatta tcaggccatt cctgagcgag atgagttgat
    15781 ggatatgctg gatgaatctg tccgctatat catgcggggc tttttgtata agtttatcct
    15841 agctcatgtt ttaggagaga ccttactacc tcctctgaag aatttagcct tgcagtcagg
    15901 tggcttcttt aatctctatg ccttggcagt tatgtatact tttggtctgg agctcttctt
    15961 tgactttgca ggttattcta tgtttgcttt ggccatctca aacttgatgg gaatccgtag
    16021 ccctatcaac tttaacaagc cctttttatc aagggattta aaggagtttt ggaatcgctg
    16081 gcatatgagt ctgtccttct ggttccgtga ctttgtcttt atgcgaatgg tgatggtgtt
    16141 aaccagaaag aaagtcttta aaaatcgtaa tgtaacctca agcatggcct acattgtaaa
    16201 tatgctgatt atgggatttt ggcatggtgt gacctggtac tatatcgcct atggactctt
    16261 tcatggacta ggcttggtca tcaatgatgc ctgggttcgc aagaaaaaaa cgctcaataa
    16321 ggaacggaaa aaagcaggga aggctgccct acctgagaat cgctggattc agttgcttgg
    16381 catggttgtc actttccatg ttgtcatgtt gtcattctta atcttttctg gattcttgaa
    16441 taatctatgg tttaaaaaat aaaaggaaat aaaatatgga tatcaaatca gaagttatcg
    16501 aaattattga tgagttgttt atggaagatg tttctgacat gatggatgaa gatctttttg
    16561 atgcaggtgt cttggatagt atgggaacgg ttgagttgat tgtggagatt gagaaccgtt
    16621 ttgacattcg tgtccctgta acagagtttg gtcgtgacga ctggaataca gctaataaaa
    16681 ttatagctgg tattgtggag ctacaaaatg cttaaacgct tatggatgat cttcggaccg
    16741 gtcttgatag ctggtttgtt ggtttttctg ctcattttct tttatcctac tgagatgcgt
    16801 cataatctag gagctgaaaa gcgttcagcg gtggctacta ctatcgatag ttttaaggag
    16861 cgaagtcaaa aagtcagagc actatctgat ccaaatgtgc gttttgttcc cttctttggc
    16921 tctagtgaat ggcttcgttt tgacggtgct catcctgcgg tattagctga gaaatacaat
    16981 cgttcctacc gtccttatct tttaggacag gggggagctg catcgcttaa ccaatatttt
    17041 ggaatgcaac agatgttacc acagctggag aataaacaag ttgtgtatgt tatctcacct
    17101 cagtggttca gtaaaaatgg ctatgatcca gcagccttcc agcagtattt taatggagac
    17161 cagttgacta gttttctgaa acatcaatct ggggatcagg ctagtcaata tgcagcgact
    17221 cgcttactgc aacagttccc aaacgtagct atgaaggacc tggttcagaa gttggcaagt
    17281 aaagaagaat tgtcgacagc agacaatgaa atgattgaat tattggctcg ttttaatgaa
    17341 cgccaagcct ccttttttgg tcagttttcg gttagaggct atgttaacta cgataagcat
    17401 gtagctaagt atttaaaaat cttgccagac cagttttctt atcaagcaat agaagatgtt
    17461 gtcaaagcag atgctgaaaa aaatacttcc aataatgaga tgggaatgga aaattatttc
    17521 tataatgagc agatcaagaa ggatttgaag aaattaaagg attctcagaa aagctttacc
    17581 tatctcaagt cgccagagta taatgacttg cagttggttt taacacagtt ttctaaatct
    17641 aaggtaaacc cgatttttat cattccacct gttaataaaa aatggatgga ctatgctggt
    17701 ctacgagagg atatgtacca acaaacggtg cagaagattc gctaccagtt agaaagtcaa
    17761 ggttttacca atatagcaga tttttctaag gacggtgggg agcctttctt tatgaaggac
    17821 accattcacc ttggttggtt gggttggttg gcttttgaca aggcagttga tcctttccta
    17881 tccaatccca caccagctcc gacttaccat ctgaatgagc gctttttcag caaagattgg
    17941 gcgacttatg atggagatgt caaagaattt caatagataa tataatatag aagagttcaa
    18001 gaatgaaact agttttcacg ttttttcttg actctttttt tgcttgtgat ataattaact
    18061 ggtaaacaaa atgtcaaaga atagtatttt tctcttaaaa aagagaagtc taaaaggaaa
    18121 ggagtcagat atgagacagc tagcaaagga tatcgatgct tttttgaatg aggtgatttt
    18181 gcaggcggaa aatcagcatg aaatcctaat aggtcattgc actagcgagg tggccctgac
    18241 caatactcag gagcatatcc ttatgctctt gtcagaggaa tctttaacaa attcagaatt
    18301 ggcccgtcgt ctcaatgtca gtcaggcggc agttaccaag gccattaagt ctttggtcaa
    18361 ggaagggatg ttggaaacat ctaaagattc taaagatgcg cgtgtgattt tttatcagtt
    18421 gactgacttg gctcgtccaa tcgctgagga gcaccaccat caccatgagc atacactttt
    18481 aacctatgaa caagtggcga ctcagtttac tccaaatgaa caaaaagtga ttcagcggtt
    18541 tttgactgct ttagtaggag aaatcaaata atgagatata ttacggtaga ggatttgtcc
    18601 ttctattatg ataaggagcc tgttcttgaa catatcaatt atagtgttga tagtggggaa
    18661 tttgttacct tgactgggga aaatggagcg gctaagacga cgctcatcaa ggccagtctt
    18721 ggaattctgc aaccacgcat tggaaaggtg gctatttcaa agacaaatac gcaaggtaag
    18781 aaattgagaa tagcctatct tcctcaacaa attgccagtt ttaatgctgg ttttccaagt
    18841 acggtctatg aatttgtcaa gtcgggtcgc tatccgagaa aaggctggtt ccgtcgtttg
    18901 aatgctcatg atgaggagca tatcaaggct agtctggact cagttggcat gtgggaacat
    18961 cgagacaaac gcttggggtc tctatctggg ggacaaaagc agcgagcggt aattgcgcgt
    19021 atgtttgctt ctgaccctga tgtgtttatc ctagacgagc cgacaacggg gatggatgca
    19081 ggaagtaaaa atgaatttta cgaactcatg caccacagcg cccatcatca tggcaaggct
    19141 gttttgatga ttacccatga ccctgaagaa gttaaggatt atgcggatcg caatattcat
    19201 ctagtccgta accaagactc gccatggcgt tgtttcaatg ttcatgagaa tggccaggag
    19261 gtgggccatg cttagtttat tatcttacga ctttatacaa cgcgcctttt tggcggttat
    19321 tgctatgagt cttttctcac cggtattggg aaccttcctc atcttgcgtc gtcagagttt
    19381 gatgagtgat acccttagcc acgtctcact ttcaggtgta gcctttggtc tggttttggg
    19441 gatttctcca actatttcta ctattgccat tgtcttgatt gcggcggtct ttctggagta
    19501 tctccgtacg gtttacaaga gctttatgga aatcgggaca gctatcctca tgtcaacagg
    19561 tctggctgtt tctctgattg tcatgagcaa gggtaaaagc tcgagttcaa tgagtttgga
    19621 ccaatatctc tttggttcga tcgtgactat cagtgaagaa caggtcattt ccctctttgt
    19681 cattgcggcg gttgttttga ttttgacctt tctctttctt cgtcctatgt atatcttaac
    19741 ttttgacgaa gatacggcct ttgtggatgg cttgccagtt cgtaccatgt ccattctttt
    19801 taacatggtg acaggggtgg ctattgccct tatgattcct gcagcaggag ctcttctggt
    19861 atcgaccatt atggtcttgc cagctagtat tgccctgcgt ctggggaaaa actttaaatc
    19921 ggttatgctg cttgccagtg cgattggctt tttgggaatg gtagcaggac tttacatttc
    19981 ctactatgca gaaacacctg caagtgcaag tattaccatt atttttgtaa ctgtctttat
    20041 actaatcagt ttagtaagac gttttatcaa ataggagacg aacgtgaaaa aaattagctt
    20101 attactagcc agtctatgtg ccttgttttt agtggcttgt tccaatcaaa aacaggcaga
    20161 tggtaaactc aatatcgtga caacctttta ccctgtctat gaatttacca agcaagtcgc
    20221 aggagatacg gctaatgtag aactcctaat cggtgctggg acagaacctc atgaatacga
    20281 accatctgcc aaggcagttg ccaaaatcca agatgcagat accttcgttt atgaaaatga
    20341 aaacatggaa acatgggtac ctaaattgct agataccttg gataagaaaa aagtgaaaac
    20401 catcaaggcg acaggcgata tgttgctctt gccaggtggc gaggaagaag agggagacca
    20461 tgaccatgga gaagaaggtc atcaccatga gtttgacccc catgtttggt tatcaccagt
    20521 tcgtgccatt aaactagtag agcacatccg cgacagcttg tcagcagatt atcctgataa
    20581 aaaagagacc tttgagaaga atgcagctgc ctatatcgaa aaattgcaat ccttggataa
    20641 ggcttacgca gaaggcttgt ctcaagcaaa acaaaagagc tttgtgactc aacacgcagc
    20701 ctttaactat cttgccttgg actatggact caaacaagtc gcaatctcag gcctttctcc
    20761 agatgcagag ccatcagctg ctcgcttggc agaattgaca gagtatgtca agaaaaataa
    20821 aatcgcctat atctattttg aagaaaatgc ctcacaagcc cttgctaaca cactttcaaa
    20881 agaagcaggt gtcaaaactg atgtcctcaa tcctttagaa agtctgacag aagaggacac
    20941 caaggctgga gaaaactaca tttccgtgat ggagaaaaac ctcaaggctt tgaaacaaac
    21001 aacagaccaa gaaggcccag caatcgaacc tgaaaaggca gaggatacca agacagtcca
    21061 aaatggttac ttcgaggatg cagctgtcaa ggaccgcacc ttgagtgact atgcaggtaa
    21121 ctggcaatca gtttatcctt tccttgaaga cggcacgttt gaccaagtct ttgactacaa
    21181 ggctaagttg actggtaaga tgacccaggc tgagtacaag gcttactata caaaaggcta
    21241 tcagacagat gtgactaaga ttaacattac tgataatact atggaatttg ttcaaggtgg
    21301 acaaagcaag aaatacactt acaagtatgt cggtaagaaa attttgactt acaagaaagg
    21361 caatcgtggc gtgcgtttcc tctttgaagc cacagatgct gacgctggac aattcaagta
    21421 tgttcagttt agtgaccaca atatcgcccc agttaaggca gaacatttcc atatcttctt
    21481 tggaggcaca agccaagaag ccctctttga agaaatggac aactggccaa cctactaccc
    21541 agataaccta tctggccaag aaatcgctca agaaatgttg gcgcattgat gagaaatcga
    21601 ctagttcata agagctagtc ggttttttga tactagatgg ctaacttcat tatagacctt
    21661 tcagaactaa gggaacctga aaaaataaat aaaactcttg aaatatctgg gcttatatgc
    21721 tagaatgtag ttagtgtata aggttcatta gaacaccaaa gcccctaact agtgccatag
    21781 ttaagggctt ttttgacgta tcttagcgat ttaacgggtg tttataggct agtcagtcgg
    21841 tctacttctt accatctagc catttgcaga cgaaatacaa gataattcca gctataacat
    21901 ctgctataat agtaagagca agctctggta taaggctcat tagtttcacc tcctctcacg
    21961 aacccatagg aacgtaatcg gtaaccgatg acaaaagtat tataccacac taaatttaga
    22021 tcatcaacct cacttaatgc ttgaactatt gtagattata tgctaaaatg tagttaattg
    22081 ataaggttca ttagaacacc aaagccccta actatggccg tagttaaggg cttttttgac
    22141 gtatcttagc gatttaacgg gtgtttatag gctagtcact cggtctactc cttactatct
    22201 agccatctgc agacgaaata caagataatt ccagctataa catccgctat aatagtaaga
    22261 gcgagctcta tgataaggct cattagtttc acctcctctc acgaacccat aggaacgtaa
    22321 tcggtaaccg atgacaaaaa tagtatacca caatacattt agatcatcaa ggtcacttaa
    22381 ttcttgaaat atagggtcta ggcgacaaag tctttaaaat cagaaaaacg cataatatca
    22441 ggtgtgcaaa aacttgatac tatgcgtttt attgtgggaa ggtttactcc attttctcct
    22501 gaaattgagt ttttgtccag cctctgtttt tagggttgct gagaaaataa tgtcatgtgg
    22561 tgaatatttg taaatcagtc agcagacaga acgaatactc ttcgaaaatc tcttcacatc
    22621 atgtcagctt cgtctttccg tgtccatggg ggacttgact tcatcagttc tatctacaac
    22681 ctcaaaacag tgtttcgagc tgacttgatc aattttcaaa tctgtacttt gagcaagctg
    22741 agactagctt cctatttgat tttcattgaa tatcagaaac tcattctcca tcaaataatt
    22801 cgactgcgtc taataatttt tggtctggca cggtgtcaga aataaaggtt gtgtatttgg
    22861 agaggggatt aattttaaaa aatccagtct tgtaaaattt agaactatca atcagtaaga
    22921 tggtttcatg ggctttgtca ataatattct tttttgaaat agcttggctg agagaagctt
    22981 cataaacata ttggtcatca atacctcttg ctgaacaaaa tgctaaatcg atattaaaat
    23041 gatctaataa agaattttcc ttatcatagt tgaccacgga acaggattga tgtttgacct
    23101 cgccagatgt gataaagatt ttggagctat ctttaacagt ttcagatagg gtttgtgcag
    23161 tatgtaaacc atttgtaaaa ataatcaaat tatcaagttc agaaagatag ggacaaagtt
    23221 cgtagacagt cgtactggaa tctagataga tacacatacc agaccgaata aagtctttag
    23281 cgagactagc gattagtctt ttttgcctag tactttctcc ttcccgtatt tgatgagaaa
    23341 gttcaattgt gttcatagag gacagggtca cgtatccgtg ctttcttttg ataagacctt
    23401 gattttctaa gaaaattaaa tcacgacgta aggtacttgt gctggagaaa gtgatttctg
    23461 ccagctcttt tacggcaatt ctttttttct ttttgatgat ttcaatcaat tcaagtacac
    23521 gttcatcttt tatcataagc tcctcctaat ttatcatttc aactatatta tagcacaaat
    23581 tggaggaatt tgaattattt ttatgaatat tgggttaaca tttgaacatt atccaagtaa
    23641 gcgttcacat attgaaaaaa taaaatgtgg ggattataat aaacttaatc aaggacgaag
    23701 agagaagaaa aatggaagcg gttttagcaa tagatttagg tgcgacttct ggaagagcaa
    23761 tcgttggtta cctttctgaa aataaactag taatggaaga aataaatcgc ttttctaatc
    23821 tacctattag agtaaaaggg catttatctt gggatattga ctttctacta gctaaaattc
    23881 ttgaaagtat ccgcttggct aatactagtt acaagatttt atctatcggt attgacacat
    23941 ggggagttga ttttggactg attgataatg aaggtaagct gttatcacaa cctgttcatt
    24001 atcgtgatga aagaacaaag ggagtgttaa aggaaatatc tgaaatgact gaattagaaa
    24061 aactgtattc agagacagga aatcagatta tggagataaa taccttgttt caactcttta
    24121 aggcacgtca agaatctcct gactctttct ataagaccaa taagattctt ttaatgccag
    24181 atttgtttaa ttatctcttg acaggtaagt ttgctacaga aaaaagcatt gcttcaacaa
    24241 ctcaattatt tgatcctagg agtcaaaatt ggaatcagaa tatcttaaaa ctatttgaat
    24301 tggatttatc tttacttcct gaaattgttt cagagggaaa tgttcttgga aggataaaag
    24361 aggagtatgg tttaggcgat attcctgttg tgaatgtttg tagtcatgat acagcaagtg
    24421 cgattgtctc agtacctaag acagaaggta gtttatttat ttcatcaggt acttggtctt
    24481 tggttggagt ggaacttact tcaccgattc ttactaccga atccttcagt tatggattta
    24541 caaatgaagt cggtaaagat ggagtgatta catttctgaa gaattgtaca gggttgtgga
    24601 tcatagagga actaagacgt tcatttgaac gaagagggaa agcctattct tttgatgata
    24661 ttaggacaat ggtggagaaa gaaaaagaaa atcttcctct gattgatact gaatcaactg
    24721 aatttgcaac agaatctgat atgcacaaga ctttgacaga atatctagct tatcatcatg
    24781 aaactagaga gtggacagat ggacaactat ttaagattgt ttatgaaagc ctagctgaaa
    24841 cgtataggaa agcgatagag ttactagaag aactaactca taaggtttat aagaggatat
    24901 atgtgattgg aggaggtgct agagccagtt actttaacca aatgattgct gatagaactg
    24961 gtaaagaggt tcttacaggt tcgactgagg gtacagctgt ggggaatatt gttgtgcagc
    25021 tattgagtca aggtaaaata aacgaggata cagagttaaa ggatattatg acaaatattg
    25081 ctgatacaaa atattattat cctcagctat cataaacaaa aataaaaaaa cggaggtttt
    25141 gtcatgaaca aaaaaagtct tttgaaatgc gctgttatcg ggttagtagc tacctttggt
    25201 ttagcagctt gtggaacatc taaggatgca agcggtggaa gttcttctgg taaagaagta
    25261 ttagaatttt atcatgggta tcatcatagt gaagatgaat ggcctgtagc gaagactatg
    25321 cgagatttat atgataaatt tgcagaagaa cataaagata gtggtgtaga atttaaacca
    25381 actcctgtaa atggtgattt gaaggatata atgaataaca aggttgctag tggtgaattt
    25441 cctgatgtga ttgacctagc aggtaatgca gtatcattag ctgcgattga acaaaaactt
    25501 gttttagatt tgaaaccata tatcgattca aataagcttg aaaaaaatgt tggtttgaac
    25561 tataagcaaa accaaaaaga tggaaaaatt tatacagttc acgaacagtt atttacaatg
    25621 ggtttgtggt acaacaagga catatttgca aaagcgggtg ctaagacacc tgatcagtgg
    25681 aatacttggg atgatttcac tcaggcaatg gcaagcatta gaaaacaaga tggagtgtat
    25741 gcatttggtg ctggagaacc atctattcgc ttgtttaata cagtattagg aaccacagaa
    25801 aatggacgta aattattaga taaaccatta acaaaagaag gaattgaatc taaagaattt
    25861 gcagatgctt tgaaaatggt tatgaaagaa attcaagcta atggttctaa gaatgctggt
    25921 ggcgatgcca atgcttattc caaagatttc caagaaggta aatctgcagt attctttaat
    25981 ggtgtgtggg cttctggtga gatgtctaag aacccaagtc ttgcacctgg aatctatcct
    26041 gcaggtgtag caattagttc ttctggaggt ggtatcacta tctcaagtaa gatgtctgaa
    26101 gctaaacaaa aactagctct tgagtttttg aaatatatga ctagcgatga tgtacaaaaa
    26161 gtaatttttg aaaaagtagg agcaaatcct tctaatgaaa atgtaaatgt aaaagaactt
    26221 tcagaaaaga gttcagaagc tactactaaa attttaggac aagcaatcac tcaagttaag
    26281 aatgcaaaag cagttgttcc aactgtaagt gatgtttggg gtggcgatgt acacacagct
    26341 attatcaatg cattgacaga aagtgctgct gaaaatgtag atgttgatca aaaagtaaaa
    26401 tctactcaag atgtgttgaa atcattgatt ggttaattgt tttagggagt gaaaacgaag
    26461 ctcagttggc actgccaact gagcttgatt ttacttatat agttgaaaaa aggagtttgt
    26521 ttatggcaca aaaaattatg agcctacaaa ataggaagaa tcaaaaaaga cgatttatct
    26581 ttttatttct tttgccaacg ttgatttgtt tctttctatt ttatttttat tctgtagtaa
    26641 caatcttttt aacttcattt gctaaatggg attatacaaa tttaaacacc cctgagtttc
    26701 ttggatttga taaattattt gaaaattata ggtatgtttt taaagaatac ccatttttta
    26761 cagaagctct aattaattct gttcgttggg ctgtaattgg tgttattatt caagttcctt
    26821 tagctgtatc tgttgcaatt acattatcta agaaattaaa aggctggaag atatctagga
    26881 atctttatat tgtaccaagt atcatttcta gtgctgctat gggcttgatt tttcttcaaa
    26941 tctataatcc aaactatggt gttgttaacc aaattattca tctatttaat ccatcgttta
    27001 aagattcagt actgttgact ccaggattaa atatagtagc tatgactggc gcttatatct
    27061 tctttgcagg agcatcaacc attatgattc ttgggcaaat ttttgctatt ccagaagaag
    27121 ttcaagaagc tgctatttta gacaatatta ctggttggag gaaagagtgg tatattacga
    27181 ttccgatgat taaggggaca attaaaactg tttcaattat ggcagcaact tcaggatttt
    27241 tgctctataa cgaagtattc tttttgacaa atggtgctgc aggaacaaaa agtatcagtt
    27301 ttgttattcg agaattagca gtggctagct cacgaactca gtatgctcgt gcaaatacaa
    27361 ttggagttat acaaatctta ggtggaatgt tgattatcgt ttgtattaat attttattca
    27421 gagaaagaaa aagactgaaa ggtgagaaat gattatgaat acacatataa atggtattag
    27481 taaaaaaggc aaagttctta tatatggtta tatgctcctt accattttaa tttctatttt
    27541 ccctattgcg tggatttttt tatcatcatt aaaagcagat cctatgaaaa atccaggtat
    27601 tagtttaccg actgacttta ctcttgaagg ttatataaat gtttttacaa aacttcatgt
    27661 ttttacttac ttttggaata gctttaaagt agtgtctata tcagttatta ttagcattgt
    27721 tatgatctcc atgtcttcat atgttattgc taggatggaa tttagaggga agaagttggt
    27781 gacttctatg ctgtattcta ctctatttat tccagcaaca gctatgactt ttccagttta
    27841 tagattagta aatgagttgg ggatttataa taccccagtt gctctaattc tggtctattc
    27901 ttgtagtgga attgctatga gtttctttat tataaagaac tattttgaaa ttattccaaa
    27961 agaattggag gaggcagcag agattgatgg tgcgacatat gctcaaactt tttggaaagt
    28021 gatgttgcca attgctagac caggaatttt aacagcagca gtacttgcct ttattaataa
    28081 ctggaatgaa tattattggg catctatgtt ggtgattgat aaaaatgaat taacagtacc
    28141 agcattacta ggtcaattta ctacaagttt taatactaat tataatggtc tattttcagc
    28201 tattgttgta atcgtattac caccaatcat tctattcgca tttacaagta aatacttcat
    28261 tgaagcttta ggaggaggag cagtaaaagg atgaaacata cacttgaaac tatcaatagt
    28321 agaattcagt ggtttagaga agcaagattt gggatgttca ttcattgggg gttatattct
    28381 attccaggaa aaggggaatg gattcgtagt catcagaaat tatctattga agattatgaa
    28441 ccatattttc gagcttttga tcctaaggaa tataacccta gagaatgggc taaacaagca
    28501 aaagctgcag ggatgaagta tatggtttta acggctaaac atcatgatgg gttttgtttg
    28561 tttgattcaa aatttacaga ttataaagca acaaatacac ctgcaggtag agatttagtt
    28621 aaagaatttg tagatgctgt tcgagctgaa ggtttaaaag taggcttata ttttagtttg
    28681 attgactggc accatcctga tttccctaaa tatgctgatc taaatcatcc aatgagagga
    28741 aatgaagtct atcgagatga gaaaattaac tttgacagtt atttggagta tttgcacaat
    28801 caagttaaag aaattgtaac tggttatggt cagattgaca tattatggtt tgattattct
    28861 tatgaagaca tggtgggtga aaaatgggga gcttcaaaat taatagacat ggttcgtcat
    28921 tatcagccaa acgttatcgt agataataga ttagaaacat caggtgaagg attcggaagt
    28981 atcgttacag atgaaattac ctcttacgct ggagattttg ttagtccaga gcaaattgtt
    29041 cctcacgaag gtattcgaaa ctttaaaggt gaacctgttc cttgggaact ttgtctaact
    29101 atgaataaca attgggctta taatcctaca gattatctat ataagtctag tcaaactttg
    29161 ataagaaagt tagtggaatg tgttagtaaa aacggaaata tgattcttaa tgtaggccca
    29221 gatgctttgg gaagaattaa tgattcaagt aagaaaatat tagataattt tcatcgatgg
    29281 atgtctcgaa atggtgaggc aatttatgga tgtagtggag atgaaaattt accaaaacca
    29341 gattggggat actatactcg taatggaaat acggtttatg ctcatgtatt tgaacaacct
    29401 ataggtcctt tagctcttct tggtattagt aaagaaaacg taaaaagaat gagctttctt
    29461 catgatggaa gtgaggtgaa aatttcagaa agctggacga ccaatgccta caaaggaatt
    29521 tgttttgcac agtttggaga agttccacat tttacctatc cattaccaga cttgattgat
    29581 agcgtaatca aaattgagtt gagagagtag tttatatgaa aattgaaaat aaaaatgtaa
    29641 gacgtaattt cttttgggga gagggtcgat tttatactac tgatatcgta aacaaacggg
    29701 caggagtaat gataaaaaat gtttcgaaag aggaatttac cattacactg gagaatggta
    29761 ttaaactttc ctccactcat ttttcagcaa tcgttaggga agagggagat acgcgaattc
    29821 aggtttcttt tgtttgtcct tccattcgtt tacgtttaat ctttgaatcg agggatgatg
    29881 ttctttcgaa gcaattagtt ttagaatcat ctactgaggt tattaaatct gtagaggtag
    29941 agagttttga gtttgaaaca gaagacaata ttttctatcc gaaaagacaa gattgtatta
    30001 aggaaatggc gaatttttcc ggttattatg tagaattggg acaacctgtt tatgcgaatt
    30061 ctttattctt aggaatggaa tttccaatgt ctgaaaacaa ggtagatggt agacactatg
    30121 tatcaagata ttacttggga actgttgtaa atcaagaaaa gagtttgtgg tcttgtatta
    30181 ttgggggagc atgttcttat aaaaaagaag agattcaaga ggcatttttt gaatatgttg
    30241 aaggaatagc tcaacctagt tatttccgta aacagtataa ttcctggtat gaccatatga
    30301 ccgatattac agaggaaggt attttaaaaa gtttttctga gattcgagat ggatttgaaa
    30361 atcatggagt tcatttagat gcttatgttg ttgatgatgg ttggacaaac tatcaatcag
    30421 tttgggaatt caatcataaa ttcccaaatg gtttgagaaa tattaaatat cttgtaaatg
    30481 gatttggttc aagcctagga ttgtggattg gtccccgagg tggttataat gggacagaaa
    30541 tcattatgag tgattggtta gaagcacatc cagagttaaa tattggatct aaaaatttga
    30601 tttcaaatga tgtaaacgtg gctgatttta actatctcaa tcaaatgaag aaaaagatgt
    30661 tggaatatca aaaagaattc gatatcagct attggaaaat tgatggttgg ttacttcaac
    30721 ctgacaaacc tgataagagt ggaccgcacg gtatgtatac catgacagcg gtttatgagt
    30781 tcttaattca actgttgata gatctaagaa aggagagagg aggaaaagat tgttggttaa
    30841 acttgacttc ttatgtaaat cctagtccat ggtttttaca gtgggtcaat agtttatgga
    30901 ttcaaatatc tcaagatgta ggctttacag agaatgcagg taatgatatc aatcgtatga
    30961 ttacttaccg agatagtcag tatcaagaat ttttggaaaa acgtgagata cagttaccta
    31021 tgtggtcgct ttataatcat gaaccaatct atgctgtcag tgcaaatacc tggtacatgg
    31081 atcatcaaat gtttgcatca ataccagatt ttgaagctta tctattattt atttcaacaa
    31141 gagggaatgc tttttgggag tttcactatt cttttgatat gtttgatgaa gagcgctgga
    31201 aagccaatgc tcgtgccgtc aaatggatag aggagaatta tcaaacacta aaatatagta
    31261 aaaaaatagg aggaagtcct gaaaaatttg aaatttatgg ttataagtgt cacaatcaga
    31321 agacttctac tgaaattctc tctctgagaa atcctgctca gattaaacaa aaaataaaaa
    31381 tagagaatct ttcgatagag aattttacca gagtaattgg agattttact attcaagaag
    31441 atgagataga attggcaccg tactctatcg tgatactgaa gaagtaaaga aaaaattgtg
    31501 aggtagagat gacgatttat attaataagg acgagaccgt ttttcatttg gcaatgaaag
    31561 atagtagtta tatttttaga attttagaaa atggggaact tcaacatcta cattttggga
    31621 aaaggattca tgtcaaggaa aattataacc aattgatggc ctataaaaaa agaggatttg
    31681 aagtatcttt ttctgaagaa tttgaggata ttcaacagtc tatgatacaa aatgaatatt
    31741 cttcatatgg gaaaggagat tttcggcatc cagcctttca agttcaagga atgaatggta
    31801 gtaggataac gacactaaaa tatcaaggtt ttgaacttga aaaagggaaa aatcgtctta
    31861 actctctacc ttcaacattt gatgatattg gtcagtgtgc ggaaacatta acgattattt
    31921 taacagattc catattagat ttaactgtta gactaaatta cacaattttt ccggaataca
    31981 atgtcttagt tagaaatacg gaatttttaa ataatagcaa taataagttg actcttttga
    32041 aagcaatgag cttacagcta gatctacctg atagtcaata tgactttatt caattttctg
    32101 gagcatggct gagggaacgt cagttatata gaacttcgct tagaccaggt attcaagcaa
    32161 tagatagctt gagatactca tcaagtcctc agcaaaatcc tttctttatg ctatcaagga
    32221 gggaaactac agagcatagt ggtgaggttt atggttttaa ctttatctat tctggaaatt
    32281 ttcaaaatat gattgaagtt gaccattttg acaccgctag agtaacggta ggaataaatc
    32341 cagtagaatt tcgtttttta ttaaatcctg ccgaaagttt tgtgacacca gaagcaattg
    32401 tgatctattc tgatcaaggg atgaatcaga tgagccaaca actatcagat ttttatcgac
    32461 atcatttagt taatcctaat ttttctcaag ctagtcgtcc tataatactc aatagttggg
    32521 aaacatttta ttttgacttg ggtacagaaa aaattttaga tttagcaaag gctgctaaag
    32581 atttagggat agaattattt gtactggatg atggttggtt tggtcatagg aaagatgaca
    32641 aaagttctct gggggattgg gtaacagata gaagtcgcct tcctgaaggt attggatttc
    32701 ttgcagatga aattcacaaa ataggtttac aatttggttt gtggtttgag cctgaaatga
    32761 tttctattga tagtgatttg tacaagaatc atgccgattg gactatccat ttgttagaca
    32821 gagagaagtc agtaggaaga aatcaatatg tgttggattt gacgagacag gaagttgttg
    32881 attacctttt tgattctatt tctaaaatca taatcaaaac aaatctggat tatatcaaat
    32941 gggatatgaa tcgtcatata acagatattt atagtattga acttgattct gaacagcaga
    33001 tggaatttgg tcatcgatat atcttaggtc tttatcagtt attagatcgt ttaataacta
    33061 agttcccttc agttctattt gaatcttgtt cttcaggtgg tggacgtttt gatttaggac
    33121 ttatgtatta tgcaccgcaa gcgtggacga gtgatgatac ggacccgata gaaagattga
    33181 aaattcagca tggaacttct tatggatatt ctccatcaat gatgacagcc catgtttcta
    33241 tttctccaaa tgaacaaagt ggaagacaaa cgagtttgga cactaggaca aatgtagctt
    33301 attttagttc tttcggttat gaattagatg ttacgagatt gtcggtagaa gaaaaagaac
    33361 aagtgagaga acaaattcag ttttataaaa aatatcgttc attgtttcaa tatggggatt
    33421 tctataggat aaacagtcct tttagttgtg attctgctag ttggcaagtt gtttcaaaag
    33481 ataaatgcca atcgatttta ttgtatgctc aattgaatag taagttgaat ccaggttata
    33541 caagagttta ttttagtggt ttagataaag ataaatgcta ttccgtctct agatttgatg
    33601 agttctttta tggagatgaa ttaatgaatg ctggaataaa agtcagttta agtaatttag
    33661 cactttgtgt tccagaatat c
//
LOCUS       DAVKAO010000017        31350 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKAO010000017 DAVKAO010000000
VERSION     DAVKAO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.017"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            355..1518
                     /locus_tag="VWX16_000816"
     CDS             355..1518
                     /locus_tag="VWX16_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9158200.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1515..2192
                     /locus_tag="VWX16_000817"
     CDS             1515..2192
                     /locus_tag="VWX16_000817"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9158201.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2182..3036
                     /locus_tag="VWX16_000818"
     CDS             2182..3036
                     /locus_tag="VWX16_000818"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9158202.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQFSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3055..4122
                     /locus_tag="VWX16_000819"
     CDS             3055..4122
                     /locus_tag="VWX16_000819"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9158203.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4132..5298
                     /gene="aroC"
                     /locus_tag="VWX16_000820"
     CDS             4132..5298
                     /gene="aroC"
                     /locus_tag="VWX16_000820"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9158204.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMYVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5308..6411
                     /locus_tag="VWX16_000821"
     CDS             5308..6411
                     /locus_tag="VWX16_000821"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9158205.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            6422..6760
                     /locus_tag="VWX16_000822"
     CDS             6422..6760
                     /locus_tag="VWX16_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9158206.1"
                     /translation="MSNIYDSANDLSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQANMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6852..8135
                     /gene="aroA"
                     /locus_tag="VWX16_000823"
     CDS             6852..8135
                     /gene="aroA"
                     /locus_tag="VWX16_000823"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9158207.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITVQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8128..8604
                     /locus_tag="VWX16_000824"
     CDS             8128..8604
                     /locus_tag="VWX16_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9158208.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQRDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8601..9449
                     /gene="pheA"
                     /locus_tag="VWX16_000825"
     CDS             8601..9449
                     /gene="pheA"
                     /locus_tag="VWX16_000825"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9158209.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9446..10720
                     /gene="psr"
                     /locus_tag="VWX16_000826"
     CDS             9446..10720
                     /gene="psr"
                     /locus_tag="VWX16_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9158210.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10999..11844
                     /locus_tag="VWX16_000827"
     CDS             10999..11844
                     /locus_tag="VWX16_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9158211.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11834..12937
                     /locus_tag="VWX16_000828"
     CDS             11834..12937
                     /locus_tag="VWX16_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9158212.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFNVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12939..13925
                     /locus_tag="VWX16_000829"
     CDS             12939..13925
                     /locus_tag="VWX16_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9158213.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAKKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGYAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13941..15374
                     /locus_tag="VWX16_000830"
     CDS             13941..15374
                     /locus_tag="VWX16_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9158214.1"
                     /translation="MIDGKRLLFSLTIVSYTLTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15403..16437
                     /locus_tag="VWX16_000831"
     CDS             15403..16437
                     /locus_tag="VWX16_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9158215.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16637..17374
                     /gene="mecA"
                     /locus_tag="VWX16_000832"
     CDS             16637..17374
                     /gene="mecA"
                     /locus_tag="VWX16_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9158216.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17525..18811
                     /locus_tag="VWX16_000833"
     CDS             17525..18811
                     /locus_tag="VWX16_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9158217.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVAFYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18813..19682
                     /locus_tag="VWX16_000834"
     CDS             18813..19682
                     /locus_tag="VWX16_000834"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9158218.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19932..20870
                     /gene="msrB"
                     /locus_tag="VWX16_000835"
     CDS             19932..20870
                     /gene="msrB"
                     /locus_tag="VWX16_000835"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9158219.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20928..22652
                     /locus_tag="VWX16_000836"
     CDS             20928..22652
                     /locus_tag="VWX16_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158220.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLIPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVKLFKGTIRSNLTLGLNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22654..24402
                     /locus_tag="VWX16_000837"
     CDS             22654..24402
                     /locus_tag="VWX16_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158221.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24659..25918
                     /locus_tag="VWX16_000838"
     CDS             24659..25918
                     /locus_tag="VWX16_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9158222.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETIA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIELLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVVVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    25994..26129
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26168..26668
                     /locus_tag="VWX16_000839"
     CDS             26168..26668
                     /locus_tag="VWX16_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9158223.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26744..27112
                     /gene="rplL"
                     /locus_tag="VWX16_000840"
     CDS             26744..27112
                     /gene="rplL"
                     /locus_tag="VWX16_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9158224.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27299..27469
                     /locus_tag="VWX16_000841"
     CDS             27299..27469
                     /locus_tag="VWX16_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158225.1"
                     /translation="MQKEGQSSRQFMKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27469..27717
                     /locus_tag="VWX16_000842"
                     /pseudo
     CDS             27469..27717
                     /locus_tag="VWX16_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27731..28054
                     /locus_tag="VWX16_000843"
     CDS             27731..28054
                     /locus_tag="VWX16_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158226.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNLW
                     EVSYD"
     gene            28047..28427
                     /locus_tag="VWX16_000844"
     CDS             28047..28427
                     /locus_tag="VWX16_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158227.1"
                     /translation="MIDLYLSKNSRRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            28440..28673
                     /locus_tag="VWX16_000845"
     CDS             28440..28673
                     /locus_tag="VWX16_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9158228.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            28676..29263
                     /locus_tag="VWX16_000846"
     CDS             28676..29263
                     /locus_tag="VWX16_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9158229.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYCASLIS
                     FLWMYAQRKQAHKVNMKSRIKWIGIGLASLLIISLCFSLIHAQESTNQANLIGLQYQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            29465..29602
                     /locus_tag="VWX16_000847"
                     /pseudo
     CDS             29465..29602
                     /locus_tag="VWX16_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
     gene            29892..31295
                     /locus_tag="VWX16_000848"
     CDS             29892..31295
                     /locus_tag="VWX16_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU9158230.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQKLESALKQYNGP
                     YILSDKRWDEVPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYLYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAASGFRSWRETFPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
BASE COUNT         9436 a         5653 c         6969 g         9292 t
ORIGIN      
        1 caaaaggctt ctctataaaa tcaaattttc cagataccta ttgaagtaga tttatttctt
       61 catctttctt ctactacctt tgtcactaaa tttgacagac acatattgct gattcaacct
      121 ctctacctat aatctcacag ttaacctgag gctagtttca tagtttgcta tttgattttc
      181 gttgataaaa agaaatgctc ctccaagacg gaagagcatt ttttattgtg attcttacac
      241 atttacttcc tagttttgca ggaagtctag tataccattt tttaaaagaa gagtaaaaaa
      301 gcaagggtag tttctttttt gagaattgtc taaaagtttg atataatggt agttatgaat
      361 agaataagag tcagcaaaag ggttgaaaag aagcttgcta aggggctagt tttactagaa
      421 gccagtgatc ttgagaatgt caatcttaag gatcaggaag tagaggtgca gggtcaggaa
      481 ggaaactttc ttgggactgc ctacctttct cagcaaaaca agggcttggg ctggtttgtc
      541 agcaaagaca aggtggcctt caatcaaact ttctttgaaa cgttgtttag aaaagccaaa
      601 gaaaagagaa acgcctacta tcaagatgat ttgacaactg cctttcgtct ctttaatcaa
      661 gagggagatg gctttggggg tctgacagtg gacctttatg gcgactacgc cgtcttttct
      721 tggtataact cttatgttta tcagattcgt cagactatat cagaagcctt tagacaggtt
      781 ttccctgagg ttttaggagc ttatgagaaa atccgcttta agggttttga ctatgaatct
      841 gcccatgttt atggtcaaga agcacctgac tttttcactg ttttagaaaa tggtgtcctg
      901 tatcaagtct ttatgaatga tggcttgatg acaggaattt tcctagacca gcatgaggtt
      961 cgcggtagtt tagttgacgg cttggctatg ggtaaatcct tgctcaatat gttttcctac
     1021 acagcggctt tttcagtagc tgcggccatg ggaggagcta gccagacaac ttctgttgat
     1081 ctagccaaac gttcacgaga attgtctcaa gcgcattttc aggcaaatgg gctcagcaca
     1141 gacgagcatc gttttatagt catggatgtc tttgagtatt tcaaatatgc caaacgcaaa
     1201 gacttgacct acgatgtgat tgtcctagat ccgcctagct ttgctcggaa taaaaaacaa
     1261 actttctctg tggccaagga ttatcacaag ttgatttccc agagtcttga gattttaaat
     1321 ccgggaggga ttatcattgc cagtactaat gctgccaatg tttcccgtca gaaatttaca
     1381 gaacaaattg ataaaggctt tgcaggaaga agttaccaga ttttaaacaa atatggtctt
     1441 ccagcagatt ttgcctataa taaaaaagat gaaagtagta attacctcaa ggtgattagt
     1501 atgaaggtta gtaaatgaaa ttaatcgttt cagtaatgcc aagaagttta gaggaggctc
     1561 aggctctgga tgccacgagg tacctggatg ccgacatcat tgaatggcgt gccgactatc
     1621 tgcctaaaga agcgattttg caggtagctc cagccatttt tgaaaaattc gcaggccgtg
     1681 agttggtttt cacgctacga actcgctccg aaggtggaga aatcgacctt tctccagaag
     1741 agtatatcca tctaatcaag gaagttgcgc aattctatca accagactat attgattttg
     1801 agtactatag ctacaaggat gtttttgagg aaatgctgga cttcccaaat cttgttttga
     1861 gttaccataa tttccaagaa acacctgaga acatgatgga aatcttgtca gagttgacga
     1921 tcctaaatcc aaaacttgtt aaggttgcgg tgatggctca cacggagcag gatgtcttag
     1981 acttgatgaa ctatacacga ggctttaaaa ccctcaatcc tgaacaggaa tatgtgacca
     2041 tttctatggg taaggtgggc aaggtctctc gtatcactgc ggatgtgact ggttcgagtt
     2101 ggtcttttgc cagtctggat gaggttagtg cccctgggca gatttctcta gctagcatga
     2161 aaaaaatcag ggagattttg gatgaagctt gatggctata cacgtttagc tgcagttgtt
     2221 gccaatccta ttaagcattc tatttctccc ttcatccaca atagagcctt tgaggcgaca
     2281 gctaccaacg gtgcttatgt ggcttgggag attgaagcga gtgacttggt agaaacagtg
     2341 gccaatattc gtcgctacca gatgtttggc atcaatctgt ccatgcccta taaggagcag
     2401 gtgattcctt atttggataa gctgagcgat gaagcgcgct tgattggtgc ggttaatacg
     2461 gttgtcaatg agaatggcaa tttaattgga tataatacag atggcaaggg attttttaag
     2521 tgcttgcctt cttttacaat ttcaggtaaa aagatgaccc tgctgggtgc aggtggtgcg
     2581 gctaaatcaa tcttggcaca ggctattttg gatggcgtca gtcagatttc ggtctttgtt
     2641 cgttccgttt ctatggaaaa aacaagacct tacctagaca agttacagga gcagacaggc
     2701 tttaaagtgg atttgtgtgc tttagaatat gtttctgaac tgcaagcaag gattgccgag
     2761 tcggatttgc tagttaatgc caccagtgtg ggcatggatg gccaattctc tcctgttcct
     2821 gaaaacatag tcttaccaga aactctctta gttgcagata tcatatacca accctttgaa
     2881 acaccgtttt tgaaatgggc tagaagtcag ggcaatccag cagtcaatgg tctgggaatg
     2941 ttgctctatc aagctgcaga agcttttcaa ctgtggacag gcaaggaaat gccgacagaa
     3001 gagatttggc agtctttaac agaaaaatac caataaagaa agggagactc tcctatgaaa
     3061 atcagaatcg atattcctca tcatccttat gatattcaga ttgaaaaagg ttgtatggcc
     3121 caggctggtc agtggttgcg agaactctgg caaccgcaaa aggtagtcat tgtgacagat
     3181 aaccatgtag cctctctcta tgcagagaag gtcaagctca gcctagaaga tgctggtttt
     3241 caggtagctg tttttgattt cttagaaggt gaagaaagaa agaatttaac tactgttcag
     3301 aaagtctatg aatttctagt caagcaaggt ctgactcgta gcgatggaat cgttgctctt
     3361 ggtggtggcg ttgttgggga cctggctgga tttgtagcct ctacctatat gcggggtatt
     3421 cactttgttc agattccgac tagtttgaca gctcaggttg attcttctat cggtggaaag
     3481 acaggtgtta acacaccatt tgctaaaaat atggtgggga cctttgccca accagatggg
     3541 gttttgattg atccacttgt tcttgaaacc ctcggaaaaa gagagttgat tgaagggatg
     3601 ggtgaagtta tcaagtatgg cttgattgag gatccagaac tgtgggctct cttgacggga
     3661 ctgaatggtt ctgttgagag tattttggaa catgcagaga ccttgattga acattcttgt
     3721 caggtgaagc gcaagatggt ggttgaagat gagttggaca atggtattcg tctttacctc
     3781 aattttggcc acactattgg ccatgccatc gaagcgactg ccggttatgg caaggtcatg
     3841 catggagagg ctgttgccat gggaatggta cagatttcca agattgctga ggaaaaaggc
     3901 ctcatgccag ctggcattac ccaatctatc acagagatgt gtcagaaatt cggcttgcct
     3961 gttgactatg aaaattggga agttgacaag ctttatcagg ctcttactca tgacaagaaa
     4021 gcgcgtggca acaccttgaa attggtcttg gtgccagagc ttggttcagc gaccattcat
     4081 ccagtttctc tggaagagat gaaagactac ttggtaaaat aaggagagtg tatgagatat
     4141 ttaactgcag gagaatcaca cggcccccgt ctaacagcta ttattgaggg aattccagct
     4201 ggacttccat tgacagctga ggatatcaat gaggacctta gacgccgtca gggtggctac
     4261 ggtcgtggtg gtcgtatgaa gattgagaat gaccaggttg tctttacttc gggcgttcgc
     4321 cacgggaaga cgacaggggc gcctattact atgtatgtca tcaataagga ccaccagaaa
     4381 tggctggaca tcatgtctgc ggaggacatt gaagaccgcc ttaaaagcaa gcggaaaatt
     4441 actcatcctc gcccaggtca tgccgatttg gttgggggga ttaagtaccg ttttgatgat
     4501 ttgcgaaatt ctttggagcg ttcatcagct cgtgaaacca ccatgcgggt ggcagttggt
     4561 gcagtagcca aacgtctctt ggctgagctg gatatggaga ttgccaacca tgtcgtggtc
     4621 tttggtggca aggaaatcga tgttcctgaa aatctgacag tcgctgaaat taagcaaaga
     4681 gctgcccagt ctgaagtttc tattgtcaac caagaacgag aacaggaaat caaggactat
     4741 attgaccaaa tcaaacgtga tggtgatacc atcggtgggg ttgtggagac agtcgtcgga
     4801 ggcgttccag ttggtcttgg ttcctatgtc caatgggata gaaaattgga tgcaagattg
     4861 gctcaagctg ttgtctctat caatgccttt aaaggggtgg aatttggtct tggctttgag
     4921 gctggttatc gtaaaggcag ccaagttatg gatgaaattc tctggtctaa agaagacggt
     4981 tatactcgcc gtaccaataa tctaggtggt tttgaaggtg gtatgactaa tgggcaaccc
     5041 atcgttgttc gtggggtcat gaaacccatt cctactcttt ataaacctct tatgagtgtg
     5101 gatatcgaaa cccacgaacc ttacaaggca accgtggaga gaagtgatcc gactgctctt
     5161 ccagctgcag gaatggtcat ggaagcagtt gtagcaacgg ttctggcgca agaaatcctc
     5221 gaaaaattct catcagataa tcttgaggaa ctaaaagaag cggtagccaa acaccgagac
     5281 tatacaaaga actattaagg agttcctatg gcaaaaacaa tctatatcgc aggtcttggg
     5341 ttgattggag cctctatggc acttggtatc aaacgcgatc atccagatta tgaaatttta
     5401 ggttataatc gtagtcaagc ttcgagagat atcgccttga aagaaggcat gattgaccgt
     5461 gcaacggatg attttgctag ttttgctcct ttggcagatg tcattatcct cagcttgcca
     5521 atcaaacaaa ctattgcttt cattaaggag ttggccaatt tggatttgcg agaaggcgtt
     5581 attatttcag atgctggttc gaccaagtca accattgtgg atgcggcgga gcagtatttg
     5641 gctggcaagt ctgttcgctt tgtcggggcc catcccatgg ctggtagtca caagacaggg
     5701 gctgcttcgg cagatgtcaa tctttttgaa aatgcctatt atatctttac accttcaagc
     5761 ctgacaagtc aggacacgct taaggaaatg aaggatctgc tttcaggtct tcatgctcgt
     5821 tttatcgaga ttgatgccaa ggagcatgat cgtgtcactt ctcagattag ccattttcct
     5881 catattttgg cttctagtct catggagcag actgcggtct atgctcaaga gcatgagatg
     5941 gcaaggcgct ttgcggcagg tggttttcga gatatgaccc gaattgcgga aagcgagcca
     6001 ggaatgtgga cctccattct cttgtccaat agcgagacca ttctggatag aattgaggat
     6061 ttcaaggaac gtttggaagc gattggtcag gccattagta agggagatga agagcaaatt
     6121 tggaactttt ttaaccaagc gcgtgaacaa cgccagacca tggaaatcca taagcgtggt
     6181 ggtgtggata gctcttacga cctctatgtt gacgttcctg atgaagaaga tgtcatcctg
     6241 cggattttgg aactgctacg tgggacttcc ttggttaaca tccacatcaa cgaggaaaat
     6301 cgtgaggata ttcacggaat cctacaaatt tcatttaaaa atgctcaaga cttggaaaga
     6361 gccgagcatc tcataacaaa aaataccgac tatacagtcg tcattaaata aggagaagaa
     6421 catgtcaaat atttacgata gtgcaaacga cctcagtcgc ggtctacgcg gattaccaga
     6481 atacaaggct gttaaagcag ctaaagatgc gattgcagca gatgctgagg caagcaaaat
     6541 ctttacagaa tatcttgcct ttcaagagga aattcaaaaa ctagcacaga caggtcaaat
     6601 gccagatgcg tccttccaag ctaatatgga aggctttggt aaacagattc aaggaaatag
     6661 cctcttgtca gaattcttta ccaagcaaca acaattggcc atttaccttt ctgacattga
     6721 aaaaattgtt ttcgaaccag tttcagaatt gctgaaataa gaaaagaact atcctaaagt
     6781 caggaatttt taaggaattt ctggcttttt atgataaaat aggtaaaagt attgcaagta
     6841 tgaggtccag tatgaaacta aaaacaaaca ttcgccattt acatggtagt atccgcgtcc
     6901 caggtgacaa gtctatcagc caccgttcca ttatctttgg aagtttggct gagggtgaga
     6961 ccaaggttta tgatattctg cgaggtgaag acgttctttc gaccatgcag gtttttcgtg
     7021 accttggtgt tgaaattgag gataaagatg gggttattac cgttcaaggt gtaggcatgg
     7081 ctggcttaaa agcgccgcaa aatgccctta atatgggaaa ttctggcacc tcgattcgcc
     7141 tgatttcagg tgtccttgct ggtgcagatt tcgaagtaga gatgtttgga gatgatagtc
     7201 tttccaaacg tcctatggac cgtgtgaccc ttccactgaa aaaaatgggc gtcagcatct
     7261 cagggcaaac tgaacgagac ttgcctcccc ttcgcttaaa agggacgaaa aacctaagac
     7321 ctattcatta tgagttgcca attgcctctg cccaagtcaa gtcagccttg atgtttgcag
     7381 ccttacaagc taagggggag tcagttatta tcgaaaaaga gtacacccgt aatcatactg
     7441 aagatatgtt gcaacaattt ggtggtcatt taagtgtgga tggtaagaaa atcacagtcc
     7501 aagggccaca aaaattgaca ggacagaagg tggtcgtacc aggagatatt tccagtgcag
     7561 ccttttggtt agtcgcaggt ttgattgctc caaattctcg tctagtgctg cagaatgtgg
     7621 ggataaacga aactcgcacc ggtattattg atgtcattcg tgccatgggt ggaaaattgg
     7681 aaataactga aatcgatcca gtcgctaaat ctgcaacctt gattgttgag tcttctgact
     7741 tgaaaggaac agagatttgt ggcgctttga ttccacgttt gattgatgaa ttgcctatta
     7801 ttgccctact tgcgacccaa gcccaaggtg taacagttat caaggatgct gaggagctca
     7861 aggtcaagga aacagaccgt attcaggttg tggcagacgc cttaaatagt atgggagcag
     7921 atattactcc tacggcagat gggatgatta tcaaaggaaa atcagctctt cacggtgcta
     7981 gagtcaatac gtttggtgac caccgtatcg gcatgatgac agctatcgca gccctattgg
     8041 ttgcagatgg agaggtggag cttgaccgtg cagaagccat caataccagc tatcctagtt
     8101 tctttgatga tttggagagc ttgattcatg gctaaggtgt tattagggtt tatgggggct
     8161 ggaaaatcga ctattgcaag aggcttggac cctaattacc ttgatatgga tgctctgatt
     8221 gagaagcgcc taggtatgtc cattgcgaat tttttcgctg aaaagggaga agcagccttt
     8281 cgtcaggtag aatcagaagt cctagctgat ttattacaaa gagaccaagt cgtgtcaact
     8341 ggaggaggag tggttatttc tcagagaaat cgtgacttac tcaagactaa tacagataac
     8401 atctacctga aagcagattt tgaaaccctc tacctacgta tcgcagctga taaggacaat
     8461 cagcgaccgc tttttctaaa taatagcaag gaagaactag tagctatttt tcaagaaaga
     8521 caggcttggt atgaggaagt ggctagtcgg gttttggatg tgaccaagct aagcccagag
     8581 gaaattatag aggaactaag atgaaaattg cttatctagg tcccaaagga tcattttcac
     8641 accacgttgt gcagacagct tttcctcatg aggaattgca ggcttttgcc aacattacag
     8701 atgtcatcaa ggcttatgag caaggattag tggactattc tgtggtgcca gttgaaaatt
     8761 ctattgaggg tagtgttcat gaaaccttgg actatctttt tcatcaggct cacatccaag
     8821 cagtagcaga aatcgttcag cctattcatc agcagttgat ggtggttcca ggccatacta
     8881 agattgaaaa gattttttca catccacagg ccttggctca aggaaagaaa ttcatcgatg
     8941 aacaatatcc agaggctcaa atcgaggtaa cagctagtac agcttatgcg gcccgtttta
     9001 tttccgaaca tccagatcag ccctttgcag caattgcacc tagaagttct gctgaagaat
     9061 atggattgga actgattgcc gaggatattc aggaaatgga agccaatttc acacgtttct
     9121 ggcttctagg agctgaaaag cctagtattc ccttgcaagc acaaactgaa aagatgagtt
     9181 tggccttgac attacctgac aaccttccag gtgcacttta taaggccctg tcgacctttg
     9241 cttggcgagg aattgacttg acaaaaattg aaagtcgtcc actcaagaca gcactgggtg
     9301 aatacttttt cattatcgat gtggattata ccgataagga cttggtccac tttgcccaaa
     9361 aagaattaga agcgattgga atccagtata aaattctggg tgcctatcct atttatccaa
     9421 tatcagacca tggaaaggag agaagatgag taaagaaaat cccttaagtc atcatgagca
     9481 gttgcgttat gattatttgc taaaaaatat tcactatctc aatgagagag aaaaaaatga
     9541 gtttgtctat ttgcaagaaa agctaactct tgctagggga aatagtagct ctagcttgga
     9601 acaagaaaga gaagagcagg ttgacttacc aagctatgcg aaccggagtc gctcacaatc
     9661 caaatcacaa gcactctctt tccctccaaa aaagaaaaga cggaagctcc gtcttaaacg
     9721 aatttttatg gtgatttttt cacttttggt ctgtgtggct ttggccatgg tattcatgtt
     9781 tttgcgtggt taccaagatg ctagcgcaaa gaaaactgct gatgcccggg cagctcaagt
     9841 agaagtcttt aatggtcagg acactagaga tggagttaat attttaatca tgggtactga
     9901 tggtcgaatc ggccagaaca gtgttgagac acgaactgac tctattatgg tattaaatgt
     9961 cgggggctca gataagaaaa tgaagctagt cagtttcatg cgtgataatt tggtctatat
    10021 agatggttat agtcaagtga ttaacggtag aaaacagaca gataacaagt taaacgtagc
    10081 ctacgagtta ggagaacaag aggggcaaaa aggggcagaa atggttcgcc aagtcttgaa
    10141 agataatttt gacttggaca ttaagtacta cgccttggtt gatttccagg cctttgccac
    10201 agcgattgac acgcttttcc ctgatggggt gacgattgat gctcaatttt caacattgaa
    10261 tgggcgtccc ctaacagaag ctacagtcgg agatgattta tacgctaccg agactgagtc
    10321 tccaacccaa accattaaag tcggaaaaca gcagatgaat ggctcgaccc tgctcaatta
    10381 tgctcgtttc cgtgatgatg atgaggcgga ttacggccgt acaaaaagac agcaacaagt
    10441 tttaacagca attttagagc aaattaaaga tcccactaaa cttttcactg gttctgaagc
    10501 tcttggaaag gtttttgcta tgacctcaac caacgtaccc tatactttcc tcctaacaaa
    10561 cggtttatct gttttggatg gagcaaaaaa tggtattgaa aaattgacga ttccagaact
    10621 gggtgactgg gtagatgcct atgatgttta tggaggcttg ggcttgctgg ttgatcaaaa
    10681 caaatatcaa accaagctcg ctcaaatggg cttgagataa gataatactc aatgaaaatc
    10741 aaagtgcaaa ttaggaagct agccgcaggc tgctaaaaac acagttttga ggttgtagat
    10801 aagactgacg aagtcagtta cctataccta cggcaaggtg aagctgacgt tgtttgaaga
    10861 gattttcgaa gagtataaga cagaaaaatc aaagtttgat agctatttgg atagcagtca
    10921 ggctttgatt ttttacagtc tgcaatagat tttcacccct tgtttgtggt ataatgaaac
    10981 gatacgatag aaagaataat gaataatatg actgatttaa aagcaattca ggctcgtagt
    11041 ctggagatgg ctgaatattt tgtggccttt tgtaaagaac atgatttgct ctgttatctc
    11101 tgtggcggag gcgctattgg tgcccttcga aacaagggat ttattccttg ggacgacgac
    11161 ctagactttt ttatgcctcg taaagattat gagaaattag cagaattatg gcctcgttat
    11221 gcagatgaac gttatttctt gtcaaagagt cacaagaatt ttgttgatcg taatcttttt
    11281 attaccattc gtgacaagga aaccacctgt atcaagcctt atcagcagga tttggatttg
    11341 ccacatggtc tggccttgga tgttttgcct ttggattatt atccgaaaaa tccagctgag
    11401 cggaaaaaac aggttcgttg ggccttgatt tattcactct tttgtgcgca aactattcca
    11461 gaaaagcatg gtgctctcat gaaatgggga agtcgcattt tactgggttt gactccaaaa
    11521 tctctccgtt atcgcatctg gaaaaaagct gagaaagaaa tgactaagta tgatttggct
    11581 gattgtgatg gcattacaga attatgctca ggtcctggct acatgagaaa caagtaccca
    11641 atcacatctt ttgaagacaa tcttttcttg ccatttgaag gaacagagat gcctattcca
    11701 atcggctatg atgtctatct cagaactgct tttggggatt atatgacgcc tccaccagca
    11761 gacaagcagg taccgcatca tgatgctgtc atcgctgata tggataagtc ttatacagaa
    11821 tacaagggag aatatggtgg ctaagaaaaa aatcttattt tttatgtggt ctttttctct
    11881 tggaggtggt gcagagaaga ttctatcaac cattgtttca aatctggatc cagaaaagta
    11941 tgatattgat attcttgaaa tggagcactt tgacaaggga tatgaatctg ttccaaagca
    12001 tgtacgcatt ttaaaatccc ttcaagatta tcgccaaacc agatggttac gagctttttt
    12061 gtggagaatg agaatttatt ttccaagact gactcgtcgt ttgcttgtaa aagatgatta
    12121 tgatgttgaa gtttctttta ccattatgaa tccaccactg ttgttctcta aaagaagaga
    12181 agtcaagaag atatcttgga ttcatggaag tattgaagaa cttcttaagg atagctctaa
    12241 aagagaatca catagaagcc agttggatgc tgcgaataca attgtaggga tttcaaaaaa
    12301 gaccagcaat tctatcaagg aagtttatcc agattatgct tctaaattac agacaatcta
    12361 caatggatat gattttcaga ctattctaga aaaatctcaa gagaagatcg atatcgagat
    12421 tgctcctcaa agtatctgta ctatcggacg gattgaggaa aataagggtt ctgaccgtgt
    12481 agtggaagtg atacgattat tacaccaaga gggaaaaaac tatcatctct attttatcgg
    12541 ggctggtgat atggaagagg aactgaaaaa acgagtcaaa gagtatgaga ttgaggacta
    12601 tgtacatttc cttggttatc aaaaaaatcc ttatcagtat ttatctcaga cgaaagttct
    12661 cttgtctatg tctaaacaag aaggctttcc tggagtgtat gtggaggcct tgagtctggg
    12721 actccctttt atctctacgg acgttggagg ggctgaggaa ttatcccaag aaggacgatt
    12781 tggacaaatc attgagagca atcaagaggc agctcaggcg attactaatt acatgacttc
    12841 tgcctcaaac tttaatgtcg atgaggctag ccaattcatt caacaattta caattacaaa
    12901 acaaatcgaa caagtagaaa aactattaga ggagtagcat ggaaactgca ttaattagtg
    12961 tgattgtgcc agtctataat gtggcgcagt acctagaaaa atcgatagct tccattcaga
    13021 agcagaccta tcaaaatctg gaaattattc ttgttgatga tggtgcaaca gatgaaagtg
    13081 gtcgcttgtg tgattcaatc gctgaacaag atgacagggt gtcagtgctt cataaaaaga
    13141 acgaaggatt gtcgcaagca cgaaatgatg ggatgaagca ggctcacggg gattatctga
    13201 tttttattga ctcagatgat tatatccatc cagaaatgat tcagagctta tatgagcaat
    13261 tagttcaaga agatgcggat gtttcaagct gtggtgtcat gaatgtctat gctaatgatg
    13321 aaagcccaca gtcagccaat caggatgact attttgtctg tgattctcaa acatttctaa
    13381 aggaatacct cataggtgaa aaaatacctg ggacgatttg caataagcta atcaagagac
    13441 aaattgcaac tgccctatcc tttcctaagg ggttgattta cgaagatgcc tattaccatt
    13501 ttgatttaat caagttggcc aagaagtatg tggttaatac taaaccctat tattactatt
    13561 tccatagagg ggatagtatt acgaccaaac cctatgcaaa gaaggattta gcctatattg
    13621 atatctacca aaagttttat aatgaagttg tgaaaaacta tcctgacttg aaagaggtcg
    13681 cttttttcag attggcctat gctcacttct ttattctgga taagatgttg ctagatgatc
    13741 agtataaaca gtttgaagcc tattctcaga ttcatcgttt tttaaaaggc tatgcttttg
    13801 ctatttctag gaatccaatt ttccgtaagg ggagaagaat tagtgctttg gccctattca
    13861 taaatatttc cttataccga ttcttattac tgaaaaatat tgaaaaatct aaaaaattac
    13921 attagaacgg gggtgaccaa gtgattgatg ggaaacgatt attatttagt ttgaccatag
    13981 tcagttatac cttgacgcta gtaagtggaa ttgtgtatct gtttaataat aataatgtta
    14041 gcttactttc tactttattg ttcttgttgg ttagtagctt aattgcttgt tggaatgata
    14101 tcaagtatta cttaatccat tttattttct atttaaccat ttttgtattt ctggtatcaa
    14161 gaccgaccat tgattatttt agggatggtg ctttggatac ctatcatcca atagcctatc
    14221 gttttgcctt tatagttgtc atgatttcga ttctgggctt gaccacagga ggcgtcctgg
    14281 ctcgttactt catagctagg aagaaaataa aagtagcaaa tataggaaat tctctaaaag
    14341 aggtttatat caagcggtta cgctttgtat cactaggagt ttttcttcta acctatcctt
    14401 tctatttcat tcggttattt gaacggctct tgtatcgttt gcagacttcc tactatgcct
    14461 actatgcaaa ttttgaaagt aaactgcctt attttaccta cattttgtct acctttacgg
    14521 tctatgcaat gtgtatgtat ctggcaacca agccaaagaa attgcaggcc acagcagtgc
    14581 ttgtctcctt tattgcagct aatactattc atttggcaat tgggacacga aatcccttta
    14641 ttttaagtat tttatttgct tttgtttatt actttatgcg ggagcaaact gaaaaaggaa
    14701 aatggattgg gtttaaagaa aagttagcga tttttgtagg ttctcctatt ctcatgttag
    14761 cgatgggagt acttaattat gtacgggata atgtccaagt ttcccataca ggtttctggg
    14821 atatcttact tgactttatc tataaacaag gaactagttt tggtgttctg gctcgaggtt
    14881 ttctatttaa cagtagcctc ccttaccgag atttccgtaa ttttactttt ggtcctgttc
    14941 ttgattattt tgcaaggggg agtttgggag ccattttcgg aggaaaagcc tttgaacata
    15001 caaccaatag tgtggaacta gctattgata gtaatagtta tgcccacaat ctatcctatc
    15061 ttgtcttgaa caaggaatac ttgaaagggc atggtatcgg aagtagttat atcatggagt
    15121 tgtataccga ctatggtatg attggagtct ttctgcttag tttcttactc ggcgtattat
    15181 ttatagccat gctgcaagta gcctatcgct caaggacaat cctatttgct ttatccctac
    15241 tcatcttgaa taatctattc tttatgccaa gaagcagctt ttcagaaagt ttcttcaatt
    15301 tatttacaat gcaattctgg ggaattgttc ttgtgattat atttgtagca aaaatgctta
    15361 caaaggaaaa ctagtatcta ctaaacaaag gagaaaaaaa tcatgtttga acattattca
    15421 gtagctgatt tgtttgcaaa tctttataaa aaacgaaaag caaatatttt agctctcatc
    15481 gctttatttg ctctcattgc tgtaccattt acaattaaag cagttaggaa taaaaacact
    15541 gtcaaagata caacaagtta ttcaacttat cttatctata aaatcactcc tccagaagat
    15601 tcggccaaaa cgattttgaa tcatcaaatt ggtggttata gtgattttta tgggaaattg
    15661 attgatggta atttgaatgg agcttatctt ttcaatgatg tagaacccag tgagttgaaa
    15721 aaaattgcca gtgaattaga cacgacggaa acaaccttga aaaattcaac gaatgactat
    15781 tggtggaaaa aattgaccgt ctactatatg attgacgatg caggggttgg tgtgaaaatt
    15841 ttgacatcaa gtaaagatgc caatgacttg ttagagaaaa aaattgatgg gttgattgag
    15901 aaatttaaac atgcttatgc aaatgtgaaa attgaaaaac tggaaaccat caactctaaa
    15961 gaattgaacg caaatggtga aacagcgctt ggcttaaatg tgaaaaatct gattcttcgt
    16021 ttagttgtta ttggagtggt ttgtgtgatt ttggttgtga tgggaaatgt gttagtttat
    16081 ctctttaatc caacaatcaa tagagtaggt gatttttctc agtatcaaat tgattttgta
    16141 acagagatta caacaattgc taacctagca gatgttttgt catacaaaaa tactggacag
    16201 gaattgacca tcgttagctc aaataaagct atcctagata aattgaaaca gagtcaagaa
    16261 gctttaaaag gaatgcattt tgtagatttg caggatgtat catctctttt ggaaagagat
    16321 acagtccttc ttgttgaaga gtacggagtg actcgttata agaaatttga gcaaagtctt
    16381 caaattctca gaaacttaaa tcgttctatc ctcggtgtag caacctttaa attataagta
    16441 ttctgatgta taaggtcttc aaaatcggct aagagccgat tttgaaggct ttttctatct
    16501 gtctgcagaa attttctctt ggagtctcct atagaaagac cctagggaaa atctatccgt
    16561 atagaggagc cttcttttga taaaatcgta aaaatctagt ataatagata gaactgaaag
    16621 tatgaggtta ctagcaatga aaatgaaaca aattagtgat acaactttaa aaatcacgat
    16681 gtctttagag gatttgatgg atcgtggaat ggagattgct gactttctcg ttcctcaaga
    16741 aaaaacagaa gagttctttt atgctatctt ggatgagcta gagatgcctg atagctttct
    16801 ggatacaggc atgttgagct tccgtgtgac tccaaaacct gataaggtag atgtctttgt
    16861 gaccaagtca aagattgacc aaaatctaga ttttgaagac ttatcggatt tgccagatat
    16921 ggaagaattg gctcaaatgt ctccagatga atttatcaaa accctggaaa aaagcatcgc
    16981 agacaaaacc aaggatgata tcgaagccat tcaatctctt gagcaagttg aagccaagga
    17041 agaagagcaa gagcaggctg aacaagaagc tgagagtaag aaagaacctt acatctacta
    17101 catcctttct tttgctaagt tggctgactt ggtagctttt gccaagacag tgacttttga
    17161 aatggaaact tctgaactct acaaaatgaa cgagcgctat tatttgacca ttttagtgga
    17221 tattgaaaat catccaagcc catatccagc ttggctgttg gcccgtatgc gcgagtttgc
    17281 agacgatagt gatatcagtc gctcagtctt acaagagtat ggtcaagtct tgatgagtca
    17341 cgatgcagtg ctcaatctgc aaaaaatcgg ctaatccttt ctggaaagct attttcagat
    17401 tttaagaaga gcgaatcgtc tgattcgttt tttcttttta tactgaaata gtgatttact
    17461 ataataggaa ttttcacaaa attctgttat aatggctata ttagaaaatt tcgaggagat
    17521 aaagatgaca gttaaaattg ctttactagg atttggtacc gttgcaagtg gtgtgccttt
    17581 cctcctaaag gaaaatggag gaaaaatcaa tcaatcagca cattcagata tcaaagttgc
    17641 taaggtattg gtcaaggatg aagatgaaaa aaatcgcttg cttgcagcag ggaatgactt
    17701 taactttgta accaatgtgg atgatatttt atcagaccag gatattacta tcgtagtgga
    17761 attgatgggg cgtattgagc ctgctaaaac ctttatcact cgtgccttgg aagctggaaa
    17821 acacgttgtt actgctaaca aggacctttt agctgtccat ggcgcagaat tgctagaaat
    17881 cgctcaagct aacaaggtag cattttacta cgaagcagca gttgctggtg ggattccaat
    17941 tcttcgtact ttagcaaatt ccttggcttc tgataaaatt acgcgcgtgc ttggagtagt
    18001 caacggaact tccaacttca tggtgaccaa gatggtggaa gaaggctggt cttacgatga
    18061 tgctcttgcg gaagcacaac gtctaggatt tgcagaaagc gatccgacga atgacgtaga
    18121 tgggattgat gcagcctaca agatggttat tttgagccaa tttgcctttg gcatgaagat
    18181 tgcctttgat gatgtagccc acaagggaat acgcaatatc acaccagaag acgtagctgt
    18241 agctcaagag cttggttacg tagtgaaatt ggttggttct attgaggaaa cttcttcagg
    18301 tattgctgca gaagtgactc caaccttcct acctaaagcg cacccacttg ctagtgtgaa
    18361 tggcgtaatg aacgctgtct ttgtagaatc tatcggtatt ggtgagtcta tgtactacgg
    18421 accaggtgcg ggtcaaaaac caactgcaac aagtgttgta gctgatattg tccgtatcgt
    18481 tcgtcgtttg aatgatggta ccattggcaa agacttcaac gaatatagcc gtgacttggt
    18541 cttggcaaat cctgaagatg tcaaagcaaa ctactatttc tcaatcttgg ctccagactc
    18601 aaaaggtcag gtcttgaagt tggctgaaat cttcaatgct caagatattt cctttaagca
    18661 aatccttcaa gatggcaaag agggtgacaa ggcgcgtgtc gttatcatca cacacaagat
    18721 taataaagcc cagcttgaaa atgtctcagc tgaattgaag aaggtttcag aattcgacct
    18781 cttgaatacc ttcaaggtgc taggagaata agatgaagat tattgtacct gcaaccagtg
    18841 ccaatatcgg gccaggtttt gactcggtcg gtgtagctgt aaccaagtat cttcaaattg
    18901 aggtctgcga agaacgagat gagtggctga ttgaacacca gattggcaaa tggattccac
    18961 atgatgagcg taatctcttg ctcaaaatcg ctttgcaaat tgtaccagac ttgcaaccaa
    19021 gacgcttgaa aatgaccagt gatgtccctt tggcgcgcgg tttgggttct tccagctcgg
    19081 ttatcgttgc tgggattgaa ctagccaacc aactgggtca actcaactta tcagaccatg
    19141 aaaaattgca gttagcgacc aagattgaag ggcatcctga caatgtggct ccagccattt
    19201 atggtaatct cgttattgca agttctgttg aagggcaagt ctctgctatc gtagcagact
    19261 ttccagagtg tgattttcta gcttacattc caaactatga attacgtact cgcgacagcc
    19321 gtagtgtctt gcctaaaaaa ttgtcttata aggaagctgt tgctgcaagt tctatcgcca
    19381 atgtagcggt tgctgccttg ttggcaggag acatggtgac cgctgggcaa gcaatcgagg
    19441 gagatctctt ccatgagcgc tatcgtcagg acttggtaag agaatttgcg atgattaagc
    19501 aagtgaccaa agaaaatggg gcctatgcaa cctacctttc tggtgctggg ccgacagtta
    19561 tggttctggc ttctcatgac aagatgccaa caattaaggc agaattggaa aagcaacctt
    19621 tcaaaggaaa actgcatgac ttgagagttg atacccaagg tgtccgtgta gaagcaaaat
    19681 aaagaataga agataggatg gggaaactct tgaccagagg gtttcatatc ctttttgtga
    19741 aaagaagttt atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggctgct
    19801 caaaacagtg ttttgaggtt gtggatagaa ctgacgaagt cagtaacata tatacggtaa
    19861 ggtgacgctg acgtggtttg aagagatttt cgaagagtat tagttaaaaa cgtgataaag
    19921 gagaaataaa gatggcagaa atttatctag caggtggttg tttttggggc ctagaggaat
    19981 atttttcacg catttctgga gtgctagaaa ccagtgttgg ctacgctaat ggtcaagtcg
    20041 aaacgaccaa ttaccagttg atcaaggaaa cagaccatgc agaaacggtc caagtgattt
    20101 acgatgagaa ggaagtgtca ctcagagaga ttttacttta ttatttccga gttatcgatc
    20161 ctctatctat caatcaacaa gggaatgacc gtggtcgcca atatcgaact gggatttatt
    20221 atcaggatga agcagatttg ccagctatct acacagtggt acaggagcag gaacgcatgc
    20281 tgggtcgaaa gattgcagta gaagtggagc aattacgcca ctacattctg gctgaagact
    20341 accaccaaga ttatctcagg aagaatcctt caggttactg tcatatcgat gtgaccgatg
    20401 ctgataagcc attgattgat gcagcaaact atgaaaagcc tagtcaagag gtgttgaagg
    20461 ccagtctatc tgaagagtct tatcgtgtca cacaagaagc tgctacagag gctccattta
    20521 ccaatgccta tgaccaaacc tttgaagagg ggatttatgt agatattacg acaggtgagc
    20581 cactcttttt tgccaaggat aagtttgctt caggttgtgg ttggccaagt tttagccgtc
    20641 cgatttccaa agagttgatt cattattaca aggatctgag ccatggaatg gagcgaattg
    20701 aagttcgttc tcgttcaggc agtgctcact tgggtcatgt tttcacagat ggaccgcggg
    20761 agttaggcgg cctccgttac tgtatcaatt ctgcttcttt acgctttgtg gccaaggatg
    20821 agatggaaaa agcaggatat ggctatctat tgccttactt aaacaaataa aacagagagt
    20881 ggggcttccc actttcttca tttctagaat atgaatagaa gggatttatg aaacacctat
    20941 tatcttactt caaaccctac atcaaggaat caattttagc ccccttgttc aagctgttag
    21001 aagctgtttt tgagctcttg gttcccatgg tgattgctgg gattgttgac caatctttac
    21061 ctcagggaga tcaaggtcat ctctggatgc agattggcct gctccttatc tttgcagtaa
    21121 ttggcgtttt agtggccttg atagctcaat tttactcagc aaaggcagca gtaggttttg
    21181 ctaaggaatt gacaaacgat ctttatcgtc atattctttc cttgcccaag gacagcagag
    21241 accgtctgac aacttctagt ttggttactc gcttgacttc ggatacctac cagattcaga
    21301 ctggtatcaa tcaattcctg cgtctctttt tacgagcgcc cattatcgtt tttggtgcca
    21361 tttttatggc ttatcgaatc tcagctgagt tgactttctg gttcttagtc atggttgcca
    21421 ttttgaccat tgtcattgta gggttatctc gattggtcaa tcctctctac agtagtctca
    21481 gaaagaaaac ggaccaactg gttcaggaaa cgcgccagca attgcaaggg atgcgggtta
    21541 ttcgtgcttt tggtcaagaa aaacgagagt tacagatttt tcaaaccctt aaccaagttt
    21601 atgctagatt acaagaaaag acaggtttct tgtctagttt attaatacct ctgacctatc
    21661 tgattgtcaa tggaactctt ctcgttatta tctggcaagg ctatatttca attcaaggag
    21721 gagtactcag tcaaggtgct ctcattgctc ttatcaatta cctcttacag attttggtgg
    21781 aattggtcaa gctagccatg ctgatcaatt ccctcaacca gtcctatatc tcagccaaga
    21841 gaatcgagga ggtctttgcg gaatctcccg aaaacatcca ttcagaatta gaacaaaagc
    21901 aagttaccag tggtcgggtt ttacaagtcc aagaattgac ttttacctat cctgatgcgg
    21961 cccagccttc tctgagagac atttcctttg atatgactca aggacaaatc cttggtatca
    22021 ttggggggac tggttctggt aaatcaagct tggtgcaact cttacttgga ctttatccag
    22081 tagacaaggg gaacattgac ctttatcaaa atggacgtag tcctcttaat ttggagcagt
    22141 ggcggtcttg gattgcctat gtacctcaaa aggtcaaact ctttaaggga actattcgtt
    22201 ccaacttgac tttaggttta aatcaagaag tatctgacca gaaactctgg caggccttgg
    22261 agattgcgca agctaaggat tttgtcagtg aaaaggaagg acttttggat gccctagttg
    22321 aggcaggggg gcgaaatttc tcaggtggac aaaaacaaag gttgtctatc gcccgagcag
    22381 tcttgcgcca agctccgttt atcatcctag atgatgcaac ctcggcactg gataccatta
    22441 cagagtccaa gctcttgaaa gctattagag aaaattttcc aaacacgagc ttaattttga
    22501 tctctcaacg aacctcaact ttacagatgg cggaccagat tctcctcttg gaaaaaggtg
    22561 agttgctagc tgttggcaag cacgatgact tgatgaaatc cagccaagtc tatcgtgaaa
    22621 tcaatgcatc ccaacatgga aaggaggact agagtgaaac gacaaactgt aaaccagacg
    22681 ctcaaacgtt tagccgtaga tttagcaaac catcccttcc tccttttcct agcctttcta
    22741 ggaactattg cccaagttgg cttatcaatt tacctaccta ttctgattgg gcaggtcatt
    22801 gaccaagtcc tagtggctgg ttcatcacca gttttttggc agatttttct ccagatgctc
    22861 ttggtggtaa taggaaatac tctggtacaa tgggccaatc ctctcctcta taatcgtcta
    22921 atcttctctt ataccagaga tttgcgagag cgaatcatcc ataagctcca tcgtttaccg
    22981 attgcttttg tagataggca aggtagtgga gagatggtta gtcgtgtaac cacggacatc
    23041 gaacagttgg cagctggctt gaccatgatt tttaaccaat ttttcattgg tgttttgatg
    23101 attctggtta gtattctagc catgctccaa attcatctcc tcatgactct cttagtcttg
    23161 ctgttgacgc cactgtccat ggtgatttca cgctttattg ccaagagatc ctatcatctc
    23221 ttccagaagc aaacagagac gaggggaatt cagactcagt tgattgaaga atcgcttagc
    23281 cagcagacta taatccagtc cttcaatgct caaacagaat ttatccaaag attgcgtgag
    23341 gctcatgaca actactcagg ctattctcag tcagccatct tttattcttc aacggtcaat
    23401 ccttcgactc gctttgtaaa tgcactcatt tatgcccttt tagctggagt aggagcttat
    23461 cgtatcatga tgggttcaac cttgaccgtc ggtcgtttag tgactttttt gaactatgtt
    23521 cagcaataca ccaagccctt taatgatatt tcttcagtgc tagctgagtt gcaaagtgct
    23581 ctggcttgcg tagagcgtat ctatggagtc ttagatagcc ctgaagtggc tgaaacaggt
    23641 aaggaagtct tgaccagtga ccaagttaag ggagctattt cctttaaaca tgtctctttt
    23701 ggctaccatc ctgaaaaaat tttgattaag gacttgtcta tcgatattcc agctggtagt
    23761 aagatagcca tcgttggtcc gacaggtgct ggaaaatcaa ctcttatcaa tctccttatg
    23821 cgtttttatc ccattagctc gggagatatc ttgctggatg ggcaatccat ttatgattat
    23881 acacgagcat cattgagaca gcagtttggt atggtgcttc aagaaacctg gctcacacaa
    23941 gggaccattc atgataatat tgcctttggc aatcctgaag ccagtcgaga gcaagtaatt
    24001 gctgctgcca aagcagctaa tgcagacttt ttcatccaac agtttccaca gggatacgat
    24061 accaagttgg aaaatgctgg agaatctctc tctgtcggcc aagctcagct cttgaccata
    24121 gcccgagtct ttctggctat tccaaagatt cttatcttag acgaggcaac ttcttccatt
    24181 gatacacgga cagaagtgct ggtacaggat gcctttgcaa aactcatgaa gggccgcaca
    24241 agcttcatca ttgctcaccg tttgtcaacc attcaggatg cggatttaat tcttgtctta
    24301 gtagatggtg atattgttga atatggtaac catcaagaac tcatggatag aaagggtaag
    24361 tattaccaaa tgcaaaaagc tgcggctttt agttctgaat aagccattct cttttgaaag
    24421 tttatggacg aaaaaagttg ccttcgagtg acttttttgt tacaatagct agaaaaattg
    24481 ttcactgtaa tactcaatga aaatcaaaga gcaaactagg aagctagccg taggttgctc
    24541 aaaacagtgt tttgaggttg cagatagaac tgacgaagtc agtaaccaca cctacggcaa
    24601 agtgaatttg aagtggtttg aagagagtac aacttgtctt ttagaaaagg agcctataat
    24661 gaaagtcttt cagcatgtaa atatcgtgac ttgtgatcaa gatttccatg tttatcttga
    24721 tggaatctta gcagtcaagg attctcaaat cgtctatgtc ggtcaagata agccagcgtt
    24781 tttagaacaa gctgagcaga ttatagacta tcagggagct tggattatgc ctggtttggt
    24841 caattgtcac acccattctg caatgacagg tctgagaggg atccgagatg acagcaatct
    24901 ccatgaatgg ctcaatgact atatctggcc agcagaatct gagtttactc ccgacatgac
    24961 taccaatgcg gtcaaagaag ccctaacaga gatgctccag tcaggaacaa caacctttaa
    25021 cgatatgtat aatcccaatg gtgtggatat ccagcaaatt tatcaggtgg tgaaaacttc
    25081 caagatgcgt tgttattttt ctccgactct cttttcttca gagacagaaa caattgctga
    25141 gactataagc agaactcgat ccatcataga cgaaatctta aaatataaaa atccaaattt
    25201 caaggttatg gtagcacctc attctccgta tagctgcagt agagacttgc tggaagcgag
    25261 tttggaaatg gcaaaagagc taaatattcc gctccatgtc catgtggcgg agaccaagga
    25321 ggagtcagga attatcctca aacggtacgg caaacgcccc cttgcttttc ttgaagaact
    25381 gggttattta gatcatccgt ccgtatttgc tcacggggtc gaattaaacg agagagaaat
    25441 tgaactcttg gcatcttctc aagtggctat cgcccacaat cctatcagta acctcaaact
    25501 ggcatcagga attgctccaa ttatccagct ccaaaaagcg ggagtagtag tcggaattgc
    25561 gactgactcg gttgcttcca ataacaatct agatatgttt gaggaaggaa ggactgcagc
    25621 tcttcttcag aagatgaaaa gtggggatgc cagccagttt ccaatcgaaa cagctctcaa
    25681 ggtactgaca atcgaagggg ctaaggtcct tggaatggaa aatcagatag gaagtctgga
    25741 agtcggcaag caagcagatt ttctggtcat tcaaccacaa gggaaaattc atctccaacc
    25801 tcaggaaaat atgctgtctc acctggttta tgcagttaaa tctagtgatg tagatgatgt
    25861 ttatatcgcc ggagaacagg ttgttaagca aggtcaagtc ctgacagtag aactttaaaa
    25921 gaaaaatcac gaaaaatttt aaaaaaagtt tgcaaaaatc ttgcattctt tttttgacta
    25981 tgctatactt atatacggtt taaaaaaact gcctaagaca gtaggggagc tcgactcata
    26041 aatatcctac cgaggacaaa acgtatcatg taaaaagaag cgtattgtac tttcgtgtct
    26101 aggtttgggc gcgtttttct ttttgaaaaa ttccccaagc aaaataatta cggaggtgaa
    26161 cacactaatg agtgaagcaa ttattgctaa aaaagcggaa ctagttgacg tagtagctga
    26221 aaaaatgaaa gctgctgcat ctatcgtcgt tgtagacgct cgtggtttga cagttgagca
    26281 agatacagtt cttcgtcgtg agcttcgtgg aagcgaagtt gagtataaag ttattaaaaa
    26341 ctcaatcttg cgtcgtgcag ctgaaaaagc tggtcttgaa gatcttgcat ctgtatttgt
    26401 tggaccatct gcagtagcat tttctaatga agatgttatc gcaccagcga aaatcttgaa
    26461 cgacttttct aaaaacgctg aagcacttga aattaaaggt ggtgcaatcg aaggcgctgt
    26521 cgcatctaaa gaagagattc ttgcacttgc aactcttcca aaccgcgaag gacttctttc
    26581 tatgctcctt tctgtacttc aagcgccagt gcgcaacgtt gctcttgcag tcaaagcggt
    26641 tgcagaaagc aaagaagacg cggcttaatc ttaagctaca cagcgtagcc tagctacgaa
    26701 aaaaactatt ataaaattta aaacttattt ggaggaaata acaatggcat tgaacattga
    26761 aaacattatt gctgaaatta aagaagcttc aatccttgaa ttgaacgacc ttgtaaaagc
    26821 tatcgaagaa gaatttggtg taactgcagc tgctcctgta gctgttgctg cagctgatgc
    26881 agctgatgct ggtgctgcta aagattcatt cgacgttgaa ttgacatctg caggcgacaa
    26941 aaaagttggc gttatcaaag ttgtacgtga aatcactggt cttggtctta aagaagctaa
    27001 agaacttgtt gacggtgcac cagcacttgt taaagaaggc gttgcaactg cagaagctga
    27061 agaaatcaaa gctaaattgg aagaagctgg agcttcagtt actcttaaat aatagagtat
    27121 cgcaatttga ttgcggaatc cctatttacc agtctttgag agcgataagc gattgataga
    27181 aactgataaa ttgatttggt ttcctgccaa aaattctttg gcaggatttt tgttttaata
    27241 aactttcggg ggtagacact agattgtcta cccctttttt tgtgtttcgg aggtaattat
    27301 gcaaaaggaa ggacagtcat cacgtcaatt catgaagagt catctcatga ctatcatggg
    27361 agttgatatt gtaaaagcaa ctcagttagt tgatgaaatg gaacaagtag gtttaatccg
    27421 atttgatgaa tttggaaatg ttggaatttt agtcttggag ggacaataat gaagcgtatt
    27481 accgctaatc aatatcagac atcagagcgt tattataagc tccccaaact tttatttgag
    27541 agtgaacggt ataagaatat gaaaccggag gttaaggtgg tttactcagt tttaaaagat
    27601 cggttggagt tgtctttgag caaaggttgg attgatgagg atgggactat ttatttgatt
    27661 tattccaatt caaatttgat ggcactttta ggctgttcaa agtcaaaatt actctccatg
    27721 tgagtttgaa gtgacatttt tagatgatta ccataaaaaa cataactacc cactatttta
    27781 cgaatcctat cttcaaaacg ttatggaatt ccttgaaagt caagacataa agaatggggt
    27841 tgatgccttt gtagatgatc atcaaaatct cgtttttgtt ttatatggac aaggctatcg
    27901 agccgaggga aaagagggaa tacttacaac ccaagtaact gtaaaagctt atgatgaaga
    27961 caagaaaccg attaacttcg caaatttatt agattcctta atcgtgtcag aatatcaaat
    28021 ggaaccgaat ctttgggagg tctcctatga ttgatctcta tctaagtaaa aatagccgaa
    28081 gaaatcaact tcttttagac ttcttccaaa actatggcat cgaggtatct tgtcattcag
    28141 tttctgaaat gacaaaggac aaattaattg agatgatgag ctattcttca gattgttttg
    28201 agtttttatc tccaaattta ttacgtttta agaatcgaga taacctaaaa ttaacagatt
    28261 tcatcgaaat gatattaaaa aatcctgaac taaccatcag acttcctctt gcggtttcaa
    28321 ataagcgagt ttatccaagt ctgaatttgg aagaggctag agctctattg ccaagagata
    28381 cgaaacaatt gatttacatg gcgcaaacac attatttatc taactaaagg agaagcaata
    28441 tggctgaacg attttgggag aacttgtcca ttattttggc tgaaagaaat atcagctgga
    28501 ttgagctaac cagaaaaatg tttgcgggag agtttcacta tccaagtgaa ttgaatcgtt
    28561 tgtatcaaaa gattcgtcac tacaagatgg aacaacgaat gcctcaaagt ccatgggtag
    28621 aaaggattgt gcaggtatta gatttagatt atgaagattt atttcggagg taactatgaa
    28681 aagaataatt ccagtttata tattccaaca agtaaatgtc ctattggtat ctctatactt
    28741 actgaaattt ctttgtatcg gtgagttaac tatactacag attctatatt gtgcgtcact
    28801 catttctttt ttatggatgt atgcccaacg taaacaagcg cataaggtca atatgaaatc
    28861 aaggataaaa tggattggta ttggattagc cagtctactg attataagtc tatgttttag
    28921 tctaatccat gcgcaagaaa gcacgaatca agcaaactta attgggcttc aatatcaagt
    28981 tccttggttt tcatttttat tgttcttaat caatgcgagt atggttgaag aatttctgta
    29041 tagagaaatt ttatggaact tggttagaaa attagatatt cgagttgctt tgacaagtgt
    29101 tttatttgcc ttagcacatc atccaggaac cattatagct tggtgtttgt atgtatcgct
    29161 ggggatgttt ttagggcttg tacgctacaa attggactta tggggcagta tggggctaca
    29221 tttggtgtgg aacctactag tttatagttt gctgcttttt taaaataggt aacctgattt
    29281 ttaggtaact tctaaaacta acttccagtt ttgggagaga gatggaagtt actttgaaaa
    29341 gttacggaaa cattattttc tatgtgacaa cattgtatca ttgcttgcat tttactgctg
    29401 gagagagtat aatgaatgtg gataaagcac ttaatcccaa aaatttaaat aggaggagtt
    29461 agctatggca gaagaagtat taaatcttta attagtatct gttcaagttg acgaaacaga
    29521 tgaagttgat ggaatgcgat ttagtacatt cagtacaaac cgttgcggta actggagtgc
    29581 ttttagttgg gaaaattgtt aattcattta aattgtaaac tttagagatt ctctggagtt
    29641 atggatgtcg ttccaatatg tgcacgttgg aatgttagtg cttatatggg gggaatccaa
    29701 aggattctcc ctttttattt taataagtct gtctagctat agatatatta agtttgatag
    29761 aatttcttgg agattttgtt atggattata atttcaattt ggagcatcct ttctttttca
    29821 ctaataatga ttatagtaca gatactagca taaagtacta agtaagtctg ccttttaatt
    29881 ggcacgaagt aatgaataat gatgagtggg tttatcaata ccctattgga aaatttgttg
    29941 aaagacaagg gtggaaaata cacattagtt cagagtataa tagttcacac gaattgttac
    30001 aagatgtagc aaaaatttgc catgaaatgc gaattccatt taaacatctt agtacagaag
    30061 acaaatttat catgcgaaac gggaaattgg ttagtcgagg attttctggt aaatttatta
    30121 catgttatcc aaatcaaaat gaattagagt cggttttgca aaagttagaa agtgccttaa
    30181 agcaatataa tgggccatat attttaagcg ataaacgttg ggatgaagta ccaatatatc
    30241 tgaggtatgg tgtgtttagg ccgtcaaggg atgatgaaaa gaaggttgct attgatgaat
    30301 taattgttgg cgatgaagtt gttaaagatg aacgtttacc agtatttaaa attccaaaag
    30361 gtatagtgcc ccctgacttt ttgaataaat ggcttgacaa gaaagataaa aaacagggag
    30421 actttccctt tattatagat aatgcaatta gattttcaaa cagtggtggg atatataatg
    30481 ctagactaaa agaagatggg aaaaaaataa ttttaaaaga ggctagaccg tatacaggtc
    30541 taggatttga tggtacttat tcttcagaga gattagcttc ggagtgcaaa gcactaaaaa
    30601 ttctaaatga atggagcgag atgcctaaaa tatacttgta cggtaagatc tgggagcaca
    30661 ccttcttagg aattgaacat atgaagggcg ttccactaaa tagatgggtt accaataatt
    30721 ttcctttata tgaagttgtt gataagacca aagattatct attgagagtt tcaaagatag
    30781 tagaaaaact catcgattta acgaataagt ttcattctga aaatgtatat catcaagatt
    30841 tacatttagg aaatatcttg gttaaggatg aagatgagat tagtataata gactgggaac
    30901 aggctgtctt ttcgaatgat gaaaaagttg tacataaagt tgcagcctct ggttttagat
    30961 catggcgaga aacatttccc agtgagatag attggtatgg tattagacaa atagctcatt
    31021 atttatacat gccactagta actacatcag atttgacata caactatgta agtcaaacca
    31081 ggatagaagg aaaaaaacta tttgagtctt tgggttatac aagagaacat atagattatg
    31141 tagaaagttt attgtcatat ctagattcta aatgtccaca gattgagaat atttccagga
    31201 aaaaagtttt aaaacccatg catgagatta gaactatcga gagtgagcaa gatatacaag
    31261 attttattat aaaagccttt cttttagtga actgataggt attttcttag caaacgataa
    31321 agttttaggg ccgatacaat caattgccta
//
LOCUS       DAVKAO010000018        30234 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKAO010000018 DAVKAO010000000
VERSION     DAVKAO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.018"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(324..2291)
                     /gene="tkt"
                     /locus_tag="VWX16_000849"
     CDS             complement(324..2291)
                     /gene="tkt"
                     /locus_tag="VWX16_000849"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9158231.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYKDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAVLPTFLGGSADLAHSNMTYIKADGLQD
                     KYNPLNRNIQFGVREFVMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQELP
                     VTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQATWHHALTSTTT
                     PTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELVL
                     QGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVIG
                     IDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            complement(2328..3023)
                     /locus_tag="VWX16_000850"
     CDS             complement(2328..3023)
                     /locus_tag="VWX16_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9158232.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKATIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            complement(3034..4119)
                     /locus_tag="VWX16_000851"
     CDS             complement(3034..4119)
                     /locus_tag="VWX16_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEU9158233.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIVGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILRKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            complement(4145..4456)
                     /locus_tag="VWX16_000852"
     CDS             complement(4145..4456)
                     /locus_tag="VWX16_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU9158234.1"
                     /translation="MKIVGVAACTMGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            complement(4484..4933)
                     /locus_tag="VWX16_000853"
     CDS             complement(4484..4933)
                     /locus_tag="VWX16_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9158235.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            complement(4911..5339)
                     /locus_tag="VWX16_000854"
     CDS             complement(4911..5339)
                     /locus_tag="VWX16_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9158236.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKPPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            complement(5332..6795)
                     /locus_tag="VWX16_000855"
     CDS             complement(5332..6795)
                     /locus_tag="VWX16_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9158237.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKG
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFRMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFSELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSAFLTEGDKQRLQAKIQEINYE"
     gene            complement(7324..9660)
                     /locus_tag="VWX16_000856"
     CDS             complement(7324..9660)
                     /locus_tag="VWX16_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9158238.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVEILARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLGY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            9793..10542
                     /locus_tag="VWX16_000857"
     CDS             9793..10542
                     /locus_tag="VWX16_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9158239.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLINRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(10834..11448)
                     /locus_tag="VWX16_000858"
     CDS             complement(10834..11448)
                     /locus_tag="VWX16_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9158240.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(12262..12531)
                     /gene="rpsO"
                     /locus_tag="VWX16_000859"
     CDS             complement(12262..12531)
                     /gene="rpsO"
                     /locus_tag="VWX16_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9158241.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(12672..13301)
                     /locus_tag="VWX16_000860"
     CDS             complement(12672..13301)
                     /locus_tag="VWX16_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9158242.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     FYIDETKTTRLLDTSDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            13540..13914
                     /locus_tag="VWX16_000861"
     CDS             13540..13914
                     /locus_tag="VWX16_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158243.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPLVILIISLLYSYRFRFSLWWLVGNGLLFYFTIITFGEFILIYLLIYETVALVGM
                     DSGISIKHILQKMKNKKLSQNP"
     gene            14171..14494
                     /locus_tag="VWX16_000862"
     CDS             14171..14494
                     /locus_tag="VWX16_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9158244.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            14552..16495
                     /gene="thrS"
                     /locus_tag="VWX16_000863"
     CDS             14552..16495
                     /gene="thrS"
                     /locus_tag="VWX16_000863"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9158245.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTEYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVV
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(16544..17518)
                     /locus_tag="VWX16_000864"
     CDS             complement(16544..17518)
                     /locus_tag="VWX16_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9158246.1"
                     /translation="MLDWKQFFLAYLRSRSRLFVYLLSLAFLVLLFQFLFASLGIYFL
                     YFFLLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLFKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(17511..18188)
                     /locus_tag="VWX16_000865"
     CDS             complement(17511..18188)
                     /locus_tag="VWX16_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9158247.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            18344..19417
                     /locus_tag="VWX16_000866"
     CDS             18344..19417
                     /locus_tag="VWX16_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9158248.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(19508..19810)
                     /locus_tag="VWX16_000867"
     CDS             complement(19508..19810)
                     /locus_tag="VWX16_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158249.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPNYLSMLFDFY"
     gene            complement(19878..20315)
                     /locus_tag="VWX16_000868"
     CDS             complement(19878..20315)
                     /locus_tag="VWX16_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9158250.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(20391..21116)
                     /locus_tag="VWX16_000869"
     CDS             complement(20391..21116)
                     /locus_tag="VWX16_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9158251.1"
                     /translation="MPGTGRRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPS
                     QRYLLRQCQYVLEEISLEEIEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTG
                     KQEEFDRLVTHPPCDIAVYGHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRA
                     QYMILEFDDKGLVDMDFRRVDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEF
                     LRELAQKEGCDRELDDWLKSGND"
     gene            21344..21994
                     /locus_tag="VWX16_000870"
     CDS             21344..21994
                     /locus_tag="VWX16_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9158252.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            22173..22850
                     /locus_tag="VWX16_000871"
     CDS             22173..22850
                     /locus_tag="VWX16_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9158253.1"
                     /translation="MVVGIAIASGYLNSRLYKFVDWGSWTALVLFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFSNIGKYIFYILNAIGALFVWNDEEVRYIE
                     GNSNKEY"
     gene            22952..23365
                     /locus_tag="VWX16_000872"
     CDS             22952..23365
                     /locus_tag="VWX16_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158254.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFECLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLGENYPDFYDKHISLWECL"
     gene            23384..23798
                     /locus_tag="VWX16_000873"
                     /pseudo
     CDS             23384..23798
                     /locus_tag="VWX16_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23837..24280)
                     /locus_tag="VWX16_000874"
     CDS             complement(23837..24280)
                     /locus_tag="VWX16_000874"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9158255.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(24310..26532)
                     /locus_tag="VWX16_000875"
     CDS             complement(24310..26532)
                     /locus_tag="VWX16_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9158256.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            26943..27185
                     /locus_tag="VWX16_000876"
     CDS             26943..27185
                     /locus_tag="VWX16_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158257.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYKKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            27525..28154
                     /locus_tag="VWX16_000877"
     CDS             27525..28154
                     /locus_tag="VWX16_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9158258.1"
                     /translation="MKIYKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(28277..30169)
                     /gene="pepO"
                     /locus_tag="VWX16_000878"
     CDS             complement(28277..30169)
                     /gene="pepO"
                     /locus_tag="VWX16_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9158259.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVATVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         9005 a         6870 c         5542 g         8817 t
ORIGIN      
        1 agttcatcat ttcaacatcc tatactatga gttttttaat tttagagacc ttctctctag
       61 ctttcaaaaa acttacaata tcctaagtca atctgtggat gctgtgttta ttgattaaag
      121 aaactatcta gatcagtagc aaagtgatta gcgtacttcg caaccgtttc agggtatact
      181 ctattgtgta tactatctac tataactttc gtagataaat tattacattc tcattatagt
      241 ttttaaataa ccaatcatct gattagtgtc tatcactaca tctgctagtc tgacaacagc
      301 tgtatcttca ttgtaattgg tttctatagg gacttaactt gagcaacgat attctctacc
      361 gtaaatccat aattatcaat cacagtctga gctggggcag acgcaccgaa gatgtcaata
      421 ccgatgaccg cgccatccaa accaacatac ttgtaccaac tttgggtcgc tgccatttca
      481 atggccacac gacgacgagt cttagatggt aaaatgtctt ccttgtaggt agcatcttga
      541 gcatcaaata gttcggttga gggcatagat accacacgta ctttaccacc ttgtaaaacc
      601 aattccttag cagctttgat agctagattg acctcagatc ctgtagcaat gataatagta
      661 tcaaatcccg gggtatcata cacgacgtag gctcctttag cgaccttacc aaagtctgtc
      721 ccttcttcaa ctaccaagtt ttgacgggtt aagacaatga cagttggagt ggtggtactg
      781 gtcaaggcat gatgccaagt cgcttgagtt tcacgggcat ccgctggacg gataacagtc
      841 aagtttggca ttgagcgtaa acctgccaaa tgttcaactg gttcatgagt tggaccatct
      901 tcaccaacgg caattgaatc atgggtaaag acataagtta caggcaactc ctgaatggct
      961 gatagccgaa tagcagcttt gacgtagtca gagaaaacaa agaaggttcc gccataaact
     1021 cgtaaaccac catgaagagc cattccattg aggattgttc ccatgacaaa ttcacgtacc
     1081 ccaaactgaa tattgcggtt taatggatta tatttatctt gtaagccatc tgccttgatg
     1141 taggtcatgt tggagtgagc taagtctgcc gatccaccta agaaggttgg taaaactgct
     1201 gctgtattaa tagcatcttg ggacgaatta cgagttgctt gagagaagcc attctcatag
     1261 acagggaagt ctttttcagt aatggttaca ggggattttc cagctacaat agcgtcaatc
     1321 tcactagcaa cttcgggata agcaaccttg taatcagaca ccaaactagc ccaagcatcg
     1381 tatgcctcct gaccacgatc cgctacattt gtcttgaaat cagaatatac ttcctctggt
     1441 acttcaaatg gatcgtaatc ccatcccaaa aacttacgag ttgctcctgt ttcttctgct
     1501 cctagtggtg caccatgaac agcatttgta ccacttttat tgggtgagcc gtaaccaatt
     1561 accgttttca cttcaatcaa actcggctta ccagaaaact tggccgtctc aattgctgta
     1621 gaaattgctg ctaaatctgt tccatcttct accagaactg tatgccaacc ataagcatcg
     1681 taacgggcgc gaacattttc agagaaagta tctttggtct caccatccaa gcagatatcg
     1741 ttggagtcgt agaggacgat aagcttatct aaagcttgat gacctgcata agaagccgcc
     1801 tccgcagaca ctccttccat gaagtcaccg tctccagcga taacataagt ataatggtca
     1861 aaaataggaa aaccatcttt gttgtactta gcagctaaaa aacgctctgc ttgggcgaaa
     1921 ccaacggcag tagaaatccc ctgaccaagc ggaccagatg tcgcatccac accagacgta
     1981 tgcgtcactt caggatgacc aggtgtctta gatccccatt gccggaaatt tttaatctcg
     2041 tccatggata catccttata ccctgttaaa tgcaagagag catacagtag cattgatcca
     2101 tgacccgcag acaagataaa gcgatctcgg ttaatccagt ttggctgctc aggtgtaatt
     2161 ctaaggtgct ttgtaaatag gctataagcc attggcgcag cacccatgac gattcccggg
     2221 tgaccagaat tagatttgtt aatagcatct acgccaagaa aacgaatggc gttaactgat
     2281 tgtagtgaca tatattcctc ctaactaata aatttcttct caaaccatta tttgattggc
     2341 attgtttttc cggtcatttc ttcgtaatct ctagaacaga tatcccaggc tttggcaata
     2401 tcgtcatcca aaccaaataa tccactgcga cctataacat aaatatctgg tcctgccaca
     2461 tcaatttgtt tgaaagtctt acgactcgaa gaaccatcca tctcaatgat gtagtgataa
     2521 ccattctgaa ctctaagctg acggagttct tggattttat acaaggtaga ctccaaaaag
     2581 cgttgtcctg caaaacctgg atctacagtc ataatagttg ctttgtcaag taaatcaatg
     2641 tagggaaaga ttgtagaaac aggtgtttca ggattaagga caacaccagc ctttagacct
     2701 gcatcatgaa ttttatcaat caaacgaaaa gcaagaccat tcagaacttc agcatgaata
     2761 caaatatact cacattgtaa atcgagaact tgatctaccc aaaaggttgg gtctgtgacc
     2821 atcagatgaa ctgataaagg tgtgtcacta attttttgaa cttcttgaat gaaccaagga
     2881 gacaaggtaa tattgggaac aaaatggcca tccataatat cgatatgata agatgctact
     2941 ttatcattca aaaaagtaat ctgctctttg aatttgtcca aatccatggt catcaaagat
     3001 ggtgaaaatt caattcttga catcgttacc ctcttacaaa atttcaatat ctgacaaatc
     3061 aatctcttct tcaacagtca taactggttc tgtatgtttt attggttttc tcaaaatcgc
     3121 gtagacaagt cctgtgacta caatgttggc taacaaagca caaataccag ctactggacg
     3181 agtcatggtt ggtaacataa gcactccacc aaatggcacg gcagaatcag cacccattgt
     3241 caaagaaaca gcaccaccaa cagcaccacc gatacctgtt gcaatgagac ctggaaccaa
     3301 gttattcata acaatcggaa ttacaccttc aacaatattg acaatcccca taggaacagc
     3361 cgatttcaat gtttcgattt cctcttgagt atagatattt tttttgagta atttcgcgat
     3421 aaaataggcc aatccaaatc caactggtgt agcagtattc accaattgca aagcagtcaa
     3481 tggttctttc acaccttcag cctgtaaagt caacacaaac gcatagactg ttttattaag
     3541 tgggccacca aagtcaacag cactgagaac tccaataact gcccccatca aaccatttga
     3601 agcacttccc aagctttgta ataagctcgt caaccagttg gtaaaggctg cgataggcgc
     3661 tccaataata taaatcataa tcaaactact taccaaagag gctacaaaag gaataatcaa
     3721 ggttggcatt aaacctttaa tccagtttgg tactttgacc tttttaataa tcgcttgaac
     3781 caagaaacca actatataac ctcccaagat accaccgata aaccctgaac caacagaatt
     3841 ggcaattaga ccaacaacaa aacctggtgc aatccctggc ttaccagcaa tcgagtaaga
     3901 caaacctgta gcaataacaa ctggcaagag accaagggct ttaccaccca tagttgctaa
     3961 agcatcccag atagtgaatt ttcctgctac aagagcgtca ggaacaccac cccccattgc
     4021 taaaccaatg gcaactaaga atcctgcacc acaaacaatt ggaatcatat aggaaatggc
     4081 tgtcaataag tgacctttta agtttaagtg tttcaacata tattttctcc ttaaatattt
     4141 tcagttattt cgtaacaatc tcaacagctt tagcaatcag tttattggga gatttgactg
     4201 ccacttctgt tggaaccttg ataatctttt taccctcaaa gcgttccata ccagaaatct
     4261 taacatcaac tgctaaaata actacatccg ctgcatcaat ctgctcttga ctcaattcat
     4321 tttctacccc tattgtcccc tgagtctcaa catgaatcac atgtccagct acctttgcgg
     4381 cattctctaa tttttcctgt gcaatataag tgtgggcaat tcccatagta caagctgcaa
     4441 caccaacaat tttcatacgg ataccctcca aaattttttc ttattaacaa aaagctgcaa
     4501 tcacatcatc agatgtctga gcccgaacta atttggcaac aacttcgtca ttaccaagtt
     4561 ttcgagcaaa gagtgataag gtcttcaaat gctccctagc agcttctgta tcatcaccaa
     4621 ctgcaaagag tacaattact ttgacccctt tcccatcaat ggtctcccaa ggaatctcat
     4681 tgtgatttat agctatgact acccccgcct tctccacagc agaactcttg ctatggggaa
     4741 tagcaatata attcccaata ccggtctgtc cttctgcctc tctctgataa agaccttcga
     4801 taaattggtc tctatcagac acataacccg tctcaaccaa tagttgagct aatgcctcaa
     4861 aaacctcttc tttgctctgc atctgtaaat ccgtctggat cagactcaca ttaagaatat
     4921 ctttgatttc catatattat ctcccgtaat tcttcttttg ttaactgttt taattgattt
     4981 atgaatgatt catctgctag tcttctcatc aatgttttaa tacatgactt gtcctgtgat
     5041 actgcaatgg ccaaaccgat aataaggtca acacactgga tatccttcga ccattctctg
     5101 ataggtggtt ttaatctagt aatcactaag acatgatgtt gaaagtttcc ttcacaatgt
     5161 ggtagaagaa cacctttagc aacctctata cttccctgtc tctcacggta atatagaagc
     5221 tcttctattt tttctgtatc ttcagaaaca agaaggctga tttgatttgc taattctttg
     5281 taggcttctt gacgattttg aacagatata tccataagga caagcgaaag attattcata
     5341 gtttatctcc tgaatttttg cttgaagacg ttgtttatca ccctcggtta gaaaagcact
     5401 aactaggaca aacgggacac ttgctggttc ctgcaaagct accgtcgtca caatgaaatc
     5461 taaatctgga tatagattta tcagctcatc taattgatga taagcaacta catcaataat
     5521 atccaattca gaaaattgct tttctaatcg tgctcgtaaa agctctgaag ttccgacacc
     5581 tgatgtacac atcactactg tttttagagg acgtgctcgc ttttcttgaa accgtgcaaa
     5641 atataagact agaaaaccaa tctcgtccag actcaaggac gcaaaaccaa atactagact
     5701 cacttcttta gaaatagttg ttaactcttt aaccagatta ggataggtta aaagaatttg
     5761 tgataaaaga ctattcttaa tctgtactct attatctaat ctcctcagta agggactgat
     5821 atgactcgcc aagtcaacaa agtcaggatc tgtcgtttca atctctctat tgtccataca
     5881 catacggcta aaatagtaat gagtgacatc taaaactcgc tgagaaaaag gaagcttccc
     5941 ggaagaaaat ggtttttgca atctcgaaga tacaacgtat tgataaagat agtcaatctc
     6001 aactgcatca accttcatcc taaaatattg ttcaatttct tgaataattt tttgacagac
     6061 actgtaaatt ttctcatcaa caatagtagg ttttgaaggt gctacaacat gaatactagt
     6121 actacgacga ttcctactga taaaaatata caggtgagag aaaatattta tattatatgg
     6181 atagggaatg tttaccccca attctctctc aagtgtaatc agtaatccca aacaaaagtg
     6241 agcaagttct ccgtcaagag gctgaacctt attctgtgta atttgctcta aatccaactg
     6301 attaaacatt ggcagtagat ttagaatggc tgaacgaatt tgagcctcat ccccatcaat
     6361 aaaaagcttt cgttgtctca tttttaaatc taacccataa attgctagac tctcttgtaa
     6421 tatctgacga tcttttagta ctactgactc gcttacgtag aattcctctc ctaaatcata
     6481 aatagaatgt ggcttaggag cagtcaacaa gagacgttct agtaattttt cacgccttac
     6541 ttcaggatca aaagcctctg taaaattccc atcaacgtcc acgagggaac ttcttggatt
     6601 taatttgaaa cctctgcctt tttcagataa aatgaatgct tctaccggac aatctttgtt
     6661 gattcccttg acaaggcgat aaaccgtttt atcagagcaa tttaattttt ctcccaatac
     6721 tttagcagat atataatcct tactatctcg caaaaggagc aacaacgttt ccatttgttc
     6781 ttgttttcta gacacgatac acctccttta tgattccatt atatcgcttt caaaaaataa
     6841 tatacttcgc attttgtcct ttgcaatgga catttttgtt tttatcaaaa atatgatatc
     6901 aaatagaaca gaacctcact atgccaaaat cattttacct tttcttcata acatctattt
     6961 tagttgaaat atttaagttg cttttcgtat ttgataagaa ataaaccaaa cgattttcta
     7021 ataaataaca ctatgttcca tttaacaata gatagcatct tcattttcaa actaccaact
     7081 tatccgacca tttgttctag caatagaaga gagttaaaat tacttcaatc tgtcgctttc
     7141 tctttcttac atcaaacttc aaatagtcta actacagata tttgaatcta ttacctcaat
     7201 ccaagcaggc ttgagaatca ctcttgggtt caccgataga tgatcggcac gtaggccttg
     7261 cacatcagat ctacgataga tccatcgtat aaaaaactgc aatccacaga ttgcagtctt
     7321 tctttatttt ctagcttgat agcgactagt caggatgaaa atcactgtaa agaccaacat
     7381 catgacacca taaacaggca acgtttgttc tgtcaaggtt gaaatttcaa gcagtttttg
     7441 gattcttggg aagagagctg tggctaggaa gcctcctact gaccagacaa tcaagaggac
     7501 tcgccataga gtgaatggca tgcaggctct aaatacggat aagaaaccaa ttgaccccaa
     7561 gagataatag aggagagttg agatttctaa ctcagaccaa ccttgactcg ttccaaagat
     7621 tttcacaaaa agaacgctaa acacgaccat gagggcactt ggtagggcac gaagcatgga
     7681 tcttctgagg aagtttggct cgacaggttt gatatttcgc tcaaaagtca aaacgaatgg
     7741 tgggaaacct tccacaaact ggtcaatcat ggtaatctgg atcggaatga aggggaaaat
     7801 caaaatccac tctgaccgac ctagtaaagc actggcaata cagatgactg ctaacaggaa
     7861 ggaatagatg gtctttatca agaaaatcgg ggcgatgtgg gcaatgttat tgaccacgcg
     7921 acgaccctcg aagagaatct caggaacatc attaaagtct gagtttaaga gaaccagatt
     7981 ggcaatctga cgggttgctg gatccccctc cgccatcacg atagaacaat ccgcctcacg
     8041 aagggccaag atatcattaa ccccgtcccc tgtcatagcc gttgtatgac ccgctttttt
     8101 caacgtttgg atgatgagtt tcttttgatg aggggaaaca cgtccgaaaa tagctgtctc
     8161 ctccgccatg gccatcaatt cctcatcggt gatttttgag caatctacat agttgtgata
     8221 gtccgcaaaa ccagccttct gggcaatgct ggacaccgta actggattgt caccagagat
     8281 aatcttgagt cccacctcct gagaacggag atagcccagc gtctctgctg ctccctctcg
     8341 aatggggtcc aagatttcca gcaaggctag agcctgaata tcagatggtt tctgtggttt
     8401 gtgatggtct aatttctcct gactgagagc taagaccaag acacgtgatc ctctctccaa
     8461 ggcctccctg gcttctggga cttcagaatc cagcaacatc tcaggtgccc ctaagaaaac
     8521 tgtccccaag ccttctaact ccatagcccc ccacttgcgg tcgctcgaga agggaagatt
     8581 ggaaatcata ggataagcaa catctcccac aaaacgctgg cgaatggctt gggcagttgg
     8641 attcttatcc tcactatggg ccatgtagct agtcaagatg ctggcaatag cctcttcacc
     8701 atacgtttcc gtcaacggaa gaacagcctc cacctgcatc tttccttggg tgatggtacc
     8761 cgtcttgtcc agacagagca tatccacgcg cgccaagatc tcaacagagt acatctcctg
     8821 caccaagacc tttttcaaac ctagcttgat aactgcagtc aagagcgaag taatggtcaa
     8881 aagggcaatt cccttaggca acattcccaa aagagctgtc gacgagttta caacagatga
     8941 cttgagaggc aggcctttta aaagcaaggc ttccagcaag agagccagac caaagggaat
     9001 gataatcttc ccagtaaaac cagccaactt gtccagcgat ttcatgatac gggagttgat
     9061 gggtttaacg gtcttagcct caagcatgag tttggcagca tagttgtctg caccgacatg
     9121 gtgaacttga gataaaactg acccactggc taggaaactt cctgacaaaa gtaagccgtc
     9181 aacttccttt tgcaccaaat cactttctcc cgttaacatg gcttcattga cttccgcaaa
     9241 gccttccaaa accaaggcat cactaggaat ctgctctcct gcagacaaac gaatgacatc
     9301 tcctagcact aattcttcag gattaagagc aacttcctga ccatcacgga tggttttgac
     9361 cttttccttg gtcatgagat tgagcttgtc caccatgtgt ttggctcgta gctcggtcac
     9421 aatcccagaa aaagcgttaa agcagataac agcaaagaag accagattgc tccaagcctg
     9481 cacaaaggca agggccaaag caatggcaaa gttcaaagcg ttaaaaaggg taaagacatt
     9541 tcgtttaacg atttgccaag tgctggtact ggctgatgcg gtaaagtcat tgaccaaacc
     9601 ctcagcctgt ctttccttga cttctctttg ggttaatccc ataatcttat ttttatccac
     9661 aaattctatt atatcatttt ttttaacaga attctaattt catccaaaat atgcatctag
     9721 tcaagggaaa agtttgccag ccctcctttg ataaggtata ataataacaa gaaaacaatc
     9781 gaaggagatc gcatgtttta tacttatttg cgtggattag ttgtattgct cttatggtcc
     9841 atcaatggca atgctcacta tcataatact gataaaattc ctaatcaaga tgaaaattat
     9901 attttagttg cgcctcaccg tacctggtgg gatcctgttt atatggcctt tgcgaccaag
     9961 ccaaaacagt tcatctttat ggcaaaaaaa gaactcttta ccaaccgtat ctttggttgg
    10021 tggattcgta tgtgtggcgc ctttcccatc gaccgtgaaa atcccagcgc ctcagccatc
    10081 aaatatccta tcaacgttct caaaaaaagt gaccgctctc tcatcatgtt tccaagtggt
    10141 agccgccact caaacgatgt caaggggggc gcagcactga ttgccaaaat ggccaaggtc
    10201 cgtatcatgc cggttaccta caccggtccc atgactttga agggcttgat taaccgtgaa
    10261 cgtgtcgata tgaactttgg aaatccaatc gatatctcag atatcaagaa aatgaatgat
    10321 gaaggcattg aaacagtcgc caatcgtatt caaacagaat tccaacgtct ggacgaagaa
    10381 acgaaacaat ggcacaatga taaaaaacca aatccactct ggtggtttat ccgcatccct
    10441 gccctcatcc ttgctattat cctcgctatc ctaaccatca tctttagctt tatcgcaagc
    10501 ttcatctgga acccagataa gaaaagagaa gaacttgcat agaagaaatg aaccttggcc
    10561 aaacagctaa ggttttcatt tgtatagtag attggaacta gaatagtaca cctctacttc
    10621 taaaacattt ttagaaatcg atttgactgt cctgatcaat ttgtcctatt cttatttcat
    10681 tttactatat ttataagaat ctcctaatca aatcaaaagc cactctacaa tagagtaact
    10741 ttaagattgt ccatttatac tcaatgaaaa agccctctga aaatcagaga gctgatttga
    10801 gctcgggcta aaatcctagt gaaacaaaaa aatctacacg atcagaaaag tctctatcgt
    10861 gccattttca tacatgatgt atagtccaag tagaatgaat actaagggca caatgattcg
    10921 ctcgtatttt tcaattgtct cgaatattaa cggaatagag gataacaccc gactaatctc
    10981 gcaaaagata attatgccga ttacaaacac aagcaaggcc acgagggtct gtgaccaatc
    11041 taacgaagca aaataaggta tatagatacc taaattatct ccgccagacg caattgtcag
    11101 caatgtaact gtccaaaaca gttgatttgc cttgctttgt tctaatcttt caataatttc
    11161 ttcctcttct tcttccgcat cttctccaac aattgcaaag cgaatcccta aatagatagg
    11221 gattaaacca agcaatccaa ccatccattc ttcaggcacg aaattaacga cataagcagc
    11281 aactaaactc gcccctacaa gtaagcctgt gcctagatat tgccccgcat aaatatgcca
    11341 tttctgttta ttctgtgata gctgtgcaaa taaaataatt aaaataatta aataatcgat
    11401 actggtggaa atataaacac caatagcaga tatgattgtc tgtcccataa aaaacctcct
    11461 gtattagtca gtaataaatc agcagatttt aggagagcac agacacatta atttagctat
    11521 cgctagtggg taatgtcgaa cgtaggaaag cattttactc ctcctcaaaa cgtagttaca
    11581 aagtaatttc taactggaat tcagtgacta cttccatgta aagtataaca tactatactt
    11641 tactttgtaa agagtgggct cttctagggg gcttgcatac agctactcgg cttgaaaagc
    11701 cgagtaactg cgcaccttta tggtgtaatt agctgatacc atttgaggtt tttgtagtct
    11761 ccaaaattgt taccgataaa acctcaataa aaaagccctc tgaagtcaga gagctgattt
    11821 tagcccgggc taaaatccta atgaaaaaga tgaaactcct tgtgctcatc gaacactgtg
    11881 tcatttccct attttcatac ggatttttga cgcccttagc atcatgattc ttgctagata
    11941 aatcgtttat agactaggcg acgaggcgaa gatcgtacga aactttcagt gaaacaaaaa
    12001 aaatctcccc gaagggagaa aagctagttt attttacctt acagtgctag gaaccgtaac
    12061 cgaagattat acttgagtat agcgaggtaa ggtgacaacg caaaaaagct acccaattta
    12121 gagtagcttt attatcaaga tatgctcaat tgaacacggc ctaagcactt ggcaaaaaag
    12181 ataaaatctc ctagaaactg aagtttcttc gtcaattttc ctatttttgc tttgtgcttt
    12241 tgacagcctt tgtatcttga attagcgacg aagtcctaga gagttgatca actcacggta
    12301 acggttaacg tcgtttttac gcaagtatgc aagcaagtta cgacggcgac cgattttttt
    12361 catcaatcca cggtaagtag cgtggtcttt tttgtgttgt ttgatgtgtt cattaaggtg
    12421 gttgatttcc caagtaagga cagcaacttg aacctctact gaacctgtat caccttcgtg
    12481 acgtgcatat tgtgcgatga tttcattttt tttctctttt gagattgcca tgatgtttct
    12541 cctttttatt tggcttcatc cgagtgacag gttggcatgc ctgtaaccaa gaagaagtta
    12601 tttgtcttta cgacaatccc tattttacca agatttaaat tttctgtcaa acattaatac
    12661 tctcatagtt attatttttc aagtaaagag atacgttctt ttatatgatg acctttttca
    12721 agttcatcta ccaatccaat agcataatct gcataactaa tctggcttat tcctttttca
    12781 ttagtagtaa aaatttctcc tgctagaatg tagtttcctg ttttttcacc atcgggaata
    12841 aaatctacag caggactgac aaaagtccag ttgagattat tttttgtacg aagaagatcc
    12901 aattcatccg cttgagcttt tgctagtggc ttaaattctt caggaaaatc agacgtatct
    12961 aaaagtcgtg tcgtttttgt ttcatctata taaaagcttc ctgatccccc tacaactaga
    13021 aaacgtgtct gtgttccagc aagaagctga ttaaatagtt cgattgattt gctgtggagc
    13081 ggtagcgtat ctggtgtata agcaccaaac gctgaaataa cagcatcaaa tccagtaaga
    13141 tcatcttttg tcaactcaaa taaatctttt ttaataatag actcagcttg acttttgttt
    13201 tcagaacgaa caatagccgt tacttcatgt cctcgtttga ctgcttcttc aacaattgcc
    13261 ttccccgctt gtccatttgc tgcaataact gctagtttca ttttttatac ctctcttgtt
    13321 gtaattattt tagttacaac aattgtgaca ctcttaataa tcaatgtcaa tagtcttgct
    13381 taattattat caaaatattt ctaccaagaa aactaaccat gattctagtg aaaagaaaaa
    13441 tcttctttgt caacaaattt actttcttgt tttaaacata ctataataat catagcaaga
    13501 gatctaagtt gtctgttttt ttaaaacgag gtgattatca tgcgtagatt ctattcccat
    13561 ctcccctact atctggtcat attattcttt tattggccac tttatgagtt gttcttgcta
    13621 gttgtttctg acccccttac actcaaggga ctctatataa acaatcttct cttctttaca
    13681 cctctggtaa tcttgattat atcgttactc tatagctacc gtttccgttt ctcactttgg
    13741 tggttagttg gtaacggact gctcttttac tttactatca taacctttgg tgagtttata
    13801 ctaatttact tgctaatcta tgaaacagtt gctctggtgg gcatggattc tggtattagc
    13861 atcaagcata ttctacaaaa aatgaaaaac aaaaaacttt cacaaaatcc ttgaaaaatc
    13921 tcacaatcat gctataataa tccatagaga caagtcactt agtccctttc tactagagag
    13981 tgcgtggttg ctggaaacgc ataggaagtc taaactgata ctactcttga gttttttatg
    14041 aaaacataaa acggtggcca cgttagagcc aatcagaggt gtccctctct tttgaggtac
    14101 ataaatgaag gtggaaccac gttgcgacgt cctttcgagg atgtcgcatt tttttattag
    14161 gatactaatt atggagttgc aagaattagt ggagcgcagt tgggcaatcc gacaagctta
    14221 tcacgaactg gaagttaagc atcatgattc caagtggacg gtagaagaag acctcttggc
    14281 tttatctaat gatattggaa atttccaacg actggtgatg acaaagcaag gacgctacta
    14341 tgatgaaaca ccctacacac tggaacaaaa actttcagaa aatatctggt ggctattaga
    14401 actttctcaa cgtttggata tagacattct gacggaaatg gaaaacttcc tctctgataa
    14461 agaaaagcaa ttgaacgtta ggacttggaa gtagtctgct gataaaaaat caatgcttag
    14521 aaactatgaa ataataaaaa aggagaacat catgattaac attactttcc cagatggcgc
    14581 tgttcgtgaa ttcgaatctg gcgtaacaac ttttgaaatt gcccaatcta tcagcaattc
    14641 cctagctaaa aaagccttgg ctggtaaatt caacggcaaa ctcatcgaca ctactcgcgc
    14701 tatcactgaa gatggaagca tcgaaattgt gacacctgat cacgaagatg cccttccaat
    14761 cttgcgtcac tcagcagctc acttgttcgc ccaagcagct cgtcgtcttt tcccagacat
    14821 tcacttggga gttggtccag ccatcgaaga tggtttctac tacgatactg acaacacagc
    14881 tggtcaaatc tctaacgaag accttcctcg tatcgaagaa gaaatgcaaa aaatcgtcaa
    14941 agaaaacttc ccatctattc gtgaagaagt gactaaagac gaggcacgtg aaatcttcaa
    15001 aaatgaccct tacaagttgg aattgattga agaacactca gaagacgaag gcggtttgac
    15061 tatctatcgt cagggtgaat atgtagacct ctgccgtgga cctcacgttc catcaacagg
    15121 tcgtatccaa atcttccacc ttctccatgt agctggtgcg tactggcgtg gaaacagcga
    15181 caacgctatg atgcaacgta tctacggtac agcttggttt gacaagaaag acttgaaaaa
    15241 ctaccttcaa atgcgtgaag aagctaagga acgtgaccac cgtaaacttg gtaaagagct
    15301 tgacctcttt atgatttcac aagaagtggg acaaggtttg ccattctggt tgccaaatgg
    15361 tgcgactatc cgtcgtgaat tggaacgcta catcgtaaac aaagagttgg cttctggcta
    15421 ccaacacgtc tacactccac cacttgcttc tgttgagctt tacaagactt ctggtcactg
    15481 ggatcattac caagaagaca tgttcccaac catggacatg ggtgacgggg aagaatttgt
    15541 ccttcgtcca atgaactgtc cgcaccacat ccaagttttc aagcaccatg ttcactctta
    15601 ccgtgaattg ccaatccgta tcgctgaaat cggtatgatg caccgttacg aaaaatctgg
    15661 tgccctcacc ggccttcaac gtgtacgtga aatgtcactc aacgacggtc acctattcgt
    15721 tactccagaa caaatccaag aagaattcca acgtgccctt cagttgatta tcgatgttta
    15781 tgaagacttc aacttgactg aataccgctt ccgcctctct cttcgtgacc ctcaagatac
    15841 tcataagtac tttgataacg atgagatgtg ggaaaatgcc caaaccatgc ttcgtgcagc
    15901 tcttgatgaa atgggcgtgg actactttga agccgaaggt gaagcagcct tctacggacc
    15961 aaaattggat atccagatta aaactgccct tggaaaagaa gaaacccttt ctactatcca
    16021 acttgatttc ttgttgccag aacgcttcga cctcaaatac atcggagctg atggcgaaga
    16081 tcaccgtcca gtcatgatcc accgtggggt tatctcaact atggaacgct tcacagctat
    16141 cttgattgag aactacaagg gtgccttccc aacatggctg gcaccacacc aagtaaccct
    16201 catcccagta tctaacgaaa aacacgtgga ctacgcttgg gaagtagtca agaaactccg
    16261 tgaccgcggt gtccgtgcag acgtagatga gcgcaatgaa aaaatgcagt tcaagatccg
    16321 tgcttcacaa accagcaaga ttccttacca attaattgtt ggagacaaag aaatggaaga
    16381 cgaaacagtc aacgttcgtc gctacggcca aaaagaaaca caaactgtct cagttgataa
    16441 ttttgttcaa gctatcctag ctgatatcgc caacaaatca cgcgttgaga aataagagtc
    16501 tagcataaaa gcctccagat tggaggcttt ttctcatcta tttttactca aggactaagt
    16561 tcacttgagc aaactgaatc cgcactgtcg ttccttttcc gacctcagac tcgatacgaa
    16621 tctggtgccc cagttcttca gaaattttct taaatagata aaggccaagt ccagaggact
    16681 gctgggtcaa acggccattg tatcctgaaa agccacgttc aaatactcgg aggacatcac
    16741 tattttttat cccgattccc gtatctttga tacaaagctc ttggtcatcc atataaatct
    16801 ccagaccacc ttccttggtg tacttgagac tgtttgagat gatttgctca ataaccacta
    16861 gcagccactt tttatccgtc acgatttctt tatcaaggtc atgtagattg acatttaagc
    16921 ctttttgaat aaagaaaaga gcatatttac gaattatttc cttgaccaag tcctcaattt
    16981 gaacctgctt taagaccaaa tcatcatgga aactttctaa acgcaggtac tgtaaaacta
    17041 ggttggtata ggagtcgatt ttgaaaattt cctgttctag ctgctgcttc agttggcggt
    17101 cgaccacttc tgcaactaag agttgactgg ctgcaatggg ggtctttatc tgatggaccc
    17161 acaaggtata gtaatccagc aaatccgtca gttttctttc tgcttttgac ctctgctgat
    17221 agagttccat ctcacgcgct tctaattttt ctgctaaagc tatttccaaa ggagacttgg
    17281 cttccctctc tccatagaga agttcctggc gatagacctg catttccacc aatatgtccc
    17341 aagtgaaaaa taagatggtt acaaagcaac acaagaggaa aaagtagagg aagtaaattc
    17401 ctagactggc aaataaaaac tgaaagagta agaccagaaa tgccaaagaa agcagataga
    17461 caaaaagacg actacgggag cgcagatagg ctagaaaaaa ttgtttccaa tcaagcatgc
    17521 ttcaatccgt accctattcc tttcttggtc tcgataaatc ctaccaatcc ctgctcctcc
    17581 aactttttac gcaaacgagc cacattgaca gagagggtat tatcatcaat gaaaaagtca
    17641 ctgttccaca gttctcgcat caggtcgtca cgtgctacga tgttgcctgc atgctcaaac
    17701 aacacgcgta aaatctggaa ttcattcttg gtcaaattca agacttgccc ttgataatgt
    17761 aaatccatgg atttggtatt gaggataaca ccagcatatt ccagcaaact ctcatcacgc
    17821 ccaaactcat aggaacgacg caacaagccc tgaaccttgg ctaaaagaac ctgctggtca
    17881 aaaggcttgg tcacaaagtc atccgctccc atattgattg ccatgacaat atccatagcc
    17941 tggtctctcg aagaaagaaa catgataggt accttggaaa tcttgcggat ttcctgacac
    18001 cagtgataac cattaaacaa gggcaaacca atatccatga ggaccagatg aggttccgac
    18061 tgaacaaata gactcaaaac ttccataaag tcttctacca ggaccacttc aaatccccat
    18121 tcagagagca ttttcccaac ctgttgacga atgacctgat catcttccac taataaaatc
    18181 ttgtgcatgc gcttctcctt ttccattatt ataacagatt tttccatgcc agatggtctg
    18241 aaactgaatt tgaaatagcc tgtttttagc cagtacaaac aggcaatgct actagctaat
    18301 ttgagggaaa tttgctaaga taaataaaaa gaaaggagct cttatggcca atatttttga
    18361 ctatctgaaa gatgtcgcat atgattctta ttacgacctt cccttgaatg agttagacat
    18421 tctaacctta atagaaatca cctacctctc ctttgataat ctggtctcca cacttcctca
    18481 acgtctttta gatctagcac ctcaggttcc aagagagccc actatgctta ctagcaaaaa
    18541 tcgccttcaa ttattagatg aattggctca acacaagcgc ttcaaaaatt gcaaactctc
    18601 ccattttatc aacgacatcg accctgaact gcaaaagcaa tttgcggcta tgacttatcg
    18661 tgtcagcctc gatacctatc tgattgtctt tcgtgggaca gatgacagta tcattggctg
    18721 gaaggaagat ttccacctga cctatatgaa ggaaattcct gctcaaaagc acgcccttcg
    18781 ctatttaaag aacttttttg ctcatcatcc taagcaaaag gttattctgg ccgggcattc
    18841 caagggagga aatctcgcta tctatgctgc tagccaaatt gagcaaagtt tgcaaaatca
    18901 gatcacagca gtttatacat ttgatgcacc tggtctccat caagaattga cacagactgc
    18961 gggttatcaa aggataatgg atagaagcaa gatattcatt ccacaaggtt ccattatcgg
    19021 tatgatgctg gaaattcctg ctcaccaaat catcgttcag agtactgccc tgggtggcat
    19081 cgcccagcac gataccttta gttggcagat tgaggacaag cacttcgtcc aactggataa
    19141 gaccaacagt gatagccagc aagtagacac aacctttaaa gaatgggtgg ccacagtccc
    19201 tgacgaagaa cttcagctct acttcgacct cttctttggc actattcttg aggctggtat
    19261 tagctctatc aatgacttgg cttccttaaa ggcgcttgaa tacattcatc atctctttgt
    19321 ccaagctcaa tccctcactc cagaagaaag agaaaccttg ggtcgcctta cccagttatt
    19381 gattgatact cgttaccagg catggaaaaa tagataatac tcttgaaaat taaatgtata
    19441 caaaacaaaa gacctagaat acatactttc atgtgcattc taagtctttt taaatagaat
    19501 ctaatagtca ataaaaatca aatagcattg agagataatt gggcttggaa cgtccctctc
    19561 gcttcaacaa aatgacccca ttatagatta aaaagatgcc acttagaaaa agcaaaaaag
    19621 gaagtaagac aaaggcaaat acataaaaag ctaactgaac attctcgtat ccatttttat
    19681 aaaaaaggta ggatagataa aaataacttg aaatgaggga taataaaaat aatactggat
    19741 tccacaaact tctattatcc ttccaaaatg acactataaa ggctaataca attcctataa
    19801 cgagatacat ttcttactcc tttaatagct acattttatc ataattatcc aaagaaaaaa
    19861 gagggcattt atccctctta atccttcatc tgactctctg catcggccac gactttttct
    19921 agactggttt gaccaagttc tgcctccata gtcaactgaa ttctctccaa tttttgatcc
    19981 aaaacatcat gaatatgagc tcctacaggg caatttggat tcggattgtc atggaaactg
    20041 aagagttgac ctgtcttacc aagacattcg accgcctgat aaacatctaa aagactaata
    20101 tccttaaggt ccttgacaat ctctgttccg cccgttccac gcgctactga aatcagctct
    20161 gccttcttca actgggacaa gatctttctg ataatgacag gattgacccc gacactagca
    20221 gccagaaaat cactggtcac cttgctttcc ttcccctcga gggcaatgat tatcagcata
    20281 tgagtcgcaa tggtaaatct acttggaatt tgcatcctct tctccttttt acgaggctac
    20341 cctgcctcta ctcttctttt tctattatta tacccttttt agttgtaatg tcaatcgtta
    20401 ccacttttca accagtcgtc taactcccga tcgcagccct ctttctgagc caattctctc
    20461 aaaaattcct gatgatgagt atggtggatc ccattgacca gactttcata gtaaacctca
    20521 aaataaggaa gtctcaggtc tttagccagc tgcaattcag ctgccacatc gtagtctacc
    20581 cgtcggaagt ccatatctac caggcccttg tcatcaaact ccaaaatcat atactgggcc
    20641 cgcaagtcct tccgtagctg agcgtccaaa aagaaaggtt ggccaatcga acccggattg
    20701 acaatcaatt gcccaccagt cccgtaacga agcaactgct ggtgaatatg tccataaaca
    20761 gcaatatcac agggaggatg agtcaccaag cggtcaaact cctcttgttt gccagtatga
    20821 atcaactctc gcccccagtt cttatcaggc agatgatggc taattcccac cgtcaaatcc
    20881 ccaaactgac gatgaatttg aagaggttga ttgtggagca cttcaatttc ttctagggaa
    20941 atttcctcta aaacatactg gcactggcgc aagagatagc gttgactggg gcgagtacta
    21001 tccaattcct tacggacacc atgccaaaga ctgtcttccc agtttcccaa aactctagcc
    21061 gtaatcggta gttgatccaa caagtccaaa atccttctac gccctgtccc tggcatgaga
    21121 atatctccca aaaccagtat tcatccactc ctatctgccg agcatctgcc aaaacagcct
    21181 ccaaggcggt ggtatttcca tgaatatctg aaagaagagc tattttcgtc atatccatct
    21241 cctcgttttt tctcttgcaa taagtataac ataaaaagtc acagctagag aaatctagct
    21301 ttttttgata tactagataa agatattaaa caagaggaaa cgaatgaccc caaacaaaga
    21361 agactatcta aaatgtattt atgaaattgg catagacctg cataagatta ccaacaagga
    21421 aattgcggct cgcatgcaag tctctccccc tgccgtaact gaaatgatca aacgaatgaa
    21481 aagtgaaaat ctcatcctaa aggacaagga atgtggctat ctactgactg acctcggtct
    21541 caaactggtc tctgagctct atcgtaagca ccgcttgatt gaagtttttc tagttcatca
    21601 tttagactat acaagtgacc agattcacga ggaagctgag gtcttggaac acactgtctc
    21661 tgacctgttc gtggaaagac tagataaact gctaggtttc cctaaaacct gcccccacgg
    21721 aggaactatt cctgccaagg gagaactcct cgttgaaatc aataacctcc cactagctga
    21781 tatcaaggaa gctggcgcct accgcctgac tcgggtgcac gatagttttg acattctcca
    21841 ttatctggac aagcactcac ttcacatcgg tgaccagctc caagtcaagc agtttgatgg
    21901 cttcagcaat accttcacta tcctcagtaa cgacgaggat ttacaagtga atatggacat
    21961 tgcaaaacaa ctctatgtcg agaaaatcaa ctaatctctc aagtccccta ccaaccctga
    22021 aagttttatt ttcaggattt tatttctatc atttgatttt actttcattt ggtggtagag
    22081 tatttgaaaa aaaaagaaag gtagtttaac ctatgaaaaa atcactaaaa atttttgcta
    22141 catctaaata gtttgacctc ttcggggttg ctttggtcgt tgggattgcg attgcatctg
    22201 gttacctcaa ctcacgcctc tataaatttg tagactgggg atcatggaca gctcttgtac
    22261 tctttggatt gatttccgta accaacgttg ggatttccat gttgtccact cgtttcacgg
    22321 ggaaattaag caaatgggga aattactttg gtattgttaa taccattttg tccggtgcta
    22381 ttgattatat ccttggaaat aaggcggcca tcattaccta tcccgtcacc ttcctcattt
    22441 atacctttgc tataaagaag tgggaagctt cgcaagaagg cagacccaac caaatgagcc
    22501 aaaaacaggt aaaattggcg gccatcatca tttccatcat cgccttcctc tttgcctttg
    22561 tgaccaacta tatcggatat gagggcaaga tgaatctcct tgcctacgta acaactattg
    22621 cctttgcact gtccctcatt gccaatgctt tgaatgcatt gaaactaaca actcagtggg
    22681 gcttttggtt gatttacaat ttcgttcagc tgacaaaagc tggtattcaa ggaaacttct
    22741 ccaatatcgg taaatacatc ttttatatcc tcaatgcaat cggagcttta tttgtctgga
    22801 atgatgaaga agttagatac atagaaggaa actcaaataa agaatactag ccaacttaag
    22861 agaatctccc ttctgctatt tttatcattt tagttgtaac tcccattctt ttggagtaga
    22921 atgaagttag ataaaagagg aaggtcttgt catgaaaaaa ctctatagaa tccattttat
    22981 agcaattgca gttattgatt tgctactatt tgcatttttt atcactagac tcgagacatc
    23041 ttttgagtgc ctcttgctct ctggtttaat tttcttcctt gctcaaggac tcctgctatt
    23101 tctcttggtc gtccgactca aacatcaatt cgctgagatt tatcctcaaa tcaataaaaa
    23161 gattcgcttc tactatttag gggttctcac cattgatttt ctattttttg ttctcttagc
    23221 cttcattagt tctcagcgtt tttcatctct tatgccaatt atcactgctt gccattctac
    23281 tttttattat atgacagctg actacctagg agaaaactat ccagactttt acgacaaaca
    23341 catctcttta tgggagtgtc tctaaggaaa aggaggtttt agcatgaaaa aaatcatctt
    23401 catcaaaacc attcaactcc ttgtcattga tggaatcatg ctggcatttt tgacatttaa
    23461 aaggggggct tacttgggac tggattttga tttatagtgg ttggctcatt ttctttcatc
    23521 ctgtgctatt gacctatctt tcaaaccaac tttgtgacca ctttagtcaa ctctattccc
    23581 agattagacc gagattctgg cgttttgctt tacaaattct cctatgggat agcctgatga
    23641 ttctctcctt ggtgtcttta agtgatattc cacttttcct tcagggaact ctcctcatcc
    23701 taggacatct catcccttcc tatcgcatct gccaaagcct gaaaagagac ttcccccaag
    23761 catatcaaga accgatttct ttttggagta ttttatgatc gatgagaaag accaagccga
    23821 ctgggcttgg tctttcttat ctctttttag tatctaggat aatggtaaca ggtccgttat
    23881 taaccagctc aacctgcata tctgctccaa agatacctgt ctgaacgggc acttcttgcg
    23941 ctaatttttg attgaaagca tcatagaagt ctgatgccat atcaggttta gctgcccctg
    24001 taaaggctgg acgattgcct ttcttagtat ccgcaaagag ggtaaactga gaaatagaga
    24061 ggatttctcc ttcaatatct ttgacagaca ggttcatctt gccttctgcg tctgaaaaaa
    24121 tccgcatatt gaccagtttt ctcacagcat agtccaaatc ttcctcttgg tcctctggtc
    24181 caacaccaac tagcaataaa agtccctgat tgatttttcc ctgaatctgg ccttctatac
    24241 tcacttgggc ttttttaacc cgttggataa tgattttcat aatagccttt ctagtaagag
    24301 ctaggacaac tagccgttgg tccgtttgac agagtaaact tctggcacac tcttaatttt
    24361 atcgacaacc gtggtcagtg tagagaggtt ggcaataccg aaggacacat ggatattagc
    24421 aaacttcata tccttggttg gttgggcatt gaccgttgaa atattcttgg ttgtatttga
    24481 aagaacttgc agtacatcgt tcaacagtcc tgtacggttg agaccgtaga tatcgatatg
    24541 ggccatatac tccttatttg agctagagta ctggtcttcc cattccacat caaggagacg
    24601 ttgctcgtag ttttcttggg cacgcaggtt catacagtcc acacggtgaa tagccacacc
    24661 acgacccttg gtaatgtagc caacaatatc gtcaccaggc acggggttac aacacttagc
    24721 aatccgcact aggagaccag aagcaccttc aataaccact cccccctcat gcttgacctt
    24781 gagagtttct ttattttcaa ccttgacctc gccacctttg acaagctcct ctgcctcagc
    24841 cttggccttg gcacgctctt cctcacggcg ttccttttca gtcagacggt taaagacggt
    24901 aatcgcaccg atttccccaa aaccaatggc cgcaaagagg gagtcttctg tcttgtaact
    24961 ggtcttttgc agaacttgat ccatgtggcg cttgtccata aatttatttg ccacatagcc
    25021 attttcttgg aactgagcca tcagcatctc acgacccttg ttgacagaca attccttatc
    25081 ttggttttta aagaactggc gaatcttatt gcgcgccttg ctagtcttga ccatattgag
    25141 ccagtcacgg ctaggtccaa aggagttcgg gttggcgata atttcaacct gatcccctgt
    25201 ctttaacttg gttgtcagtg gaaccatgcg gccattgacc ttggcaccag ttgctttttc
    25261 accgaccttg gtatggattt cgtaggcaaa atcaatcggt cctgaatctt tgggaaggga
    25321 acggacagct ccatctgggg taaaaacgta aatctcctca gccagatagt tttccttaac
    25381 agagtccaca aattccttag catcatcagc ctggtcttgg agctccatca tctccttgat
    25441 ccagttcatt ccaatagctg attccttgct gttaacttgc ccctttatac ctttcttata
    25501 agcccagtga gccgcaaccc cgtactcagc cacctcgtgc atttccttgg ttcgaatctg
    25561 gaattcaatc ggcccttttg gtccataaac agtcgtatgg atagactgat aaccattggc
    25621 cttgcggttg gcaatatagt ctttgaagcg acctggcatc ggtttccaaa attcatgcac
    25681 gtaaccaagc atggcataaa catcactttg ggtatctaaa atacaacgaa tagcaatcag
    25741 atcatagatt tcctcaaacc gttttctctt gtcctgcatt ttgcggaaaa ttgagtaaat
    25801 atgcttggga cgaccataaa tcttcccttt caagtgacgt tctgtcgtat actcctctaa
    25861 ttttgtgact acctcatcca ccaaggcctc acgctccctg cgcttttcct tcatcatatg
    25921 ggtaatcttg taaaactccg ttggattgag ataacggaaa gacaagtctt ctaattccca
    25981 tttgacactg gaaatcccca aacgatgggc aagtggggca tagatttcca tggtttcttt
    26041 ggaaatacgc tcctgcttgt cttttcgaag atgtttcagg gtccgcatat tgtgcaagcg
    26101 gtcagacagt ttgaccaaaa taacgcggat gtcctcagac atggccatga gcatcttgcg
    26161 atgattttcc gctaattgct cctcgatcga tttgtactcg accttgccaa gcttggtaac
    26221 tccgtcaaca atcacccgta catcaggacc aaactctctt tccaaatcgt ccaaggtcgc
    26281 atctgtatct tccaccacat catgcaagaa tccacaagct actgttacag catccagctt
    26341 tagcttagct aaaatacctg ccacttggat agggtgaatg atataaggct cgcctgattt
    26401 gcgatattga ccactgtggc attcaacagc atagaccaag gccttatgga caaaatgaac
    26461 atcctcttcc gttaaatatt ctttggttaa agcgacaact tcttcgcctg ttaaattcac
    26521 ttctttcggc atctctactc tccaattctt cctaccattt tatcactttt ttaagaatat
    26581 gaaaactaga ttggaacaga ataagaaaaa aataattcaa aattgcttga taattctgaa
    26641 ttattggccc ataatatact acgaagttag attttaaact taggtgatag aaggagagat
    26701 agaagaacgg aacccatatt gtaacccaaa gactttctga tttccccaat tccattgaag
    26761 atacgaaaga taaacggtgg aactcgtatc acatacactg gtaccttgac tggattttgg
    26821 aattaatact caatgaaaat caaagagcaa actaggaaac tagccgcagg ttgctcaaag
    26881 cactgctttg aggttgtaga taagactgac aaagtcagtc acatatatac ggcaaggcga
    26941 cgttgacgcg gtttgaagag atttttgaag aatataaaaa tcctcaagat actttcttct
    27001 atcctttagt ttataaggag aatacctata aaaaaactgc tatttctatc tttgctctcc
    27061 taatgttagg agtttgctgc ctgttcctat tcagccagca aagctataaa aaactcgttc
    27121 aatactatgc taacgaccag aacctgccca gtaggataac ttatagtgaa tatagcgaca
    27181 aatgagaagc caactacggt agcactctaa acatcacgtc tatcaaacaa gctaatgacg
    27241 gagtttatgc aacctatgaa gggcaattga cacctttcca atattgataa attgataacc
    27301 agcctgtctt catctagtca tgctggtttt taagttcatt ttaaatcctt acctattctc
    27361 cctaactgtg ctatacttaa tttatactca atgaaaatca aagagcaaac tagaaagcta
    27421 gccgcaggct gttcaaagca ctgctttgag gttgcagata aagttgacgc ggtttgaaga
    27481 gattttcgaa gagtattagt atattctttg agattggagc tagtatgaaa atctataaaa
    27541 ccgtgaatcc tgttgcctat gaaaatacct attatctaga aggcgaaaag cacctcatcg
    27601 tcgtcgatcc tggtagtcat tgggaagcca ttcgtcagac aatcgagaag atcaacaaac
    27661 cgatctgtgc tattctcttg acccacgccc attatgacca tatcatgagt ctggacttgg
    27721 ttcgcgagac gtttggcaat cctcctgtct atatcgcaga gagcgaagcc agctggctct
    27781 acactcctgt cgataatctc tccggtctcc ctcgccacga tgatatggca gatgtggtca
    27841 caaaacctgc agaacacacc tttgtctttc acgaagaata ccaactagag gaatttcgtt
    27901 ttaaggttct accgacccca gggcactcta tcggtggtgt ttccctagtc tttcctgatg
    27961 ctcacctagt cttgacggga gatgctctat tccgcgaaac tatcggacgg accgaccttc
    28021 cgactggtag catggagcaa ctccttcata gtatccagac ccaactcttc accctaccaa
    28081 actacgatgt ctatccagga catggtccag ctactactat cgctcacgaa aaggccttca
    28141 atcccttttt ttagcaagat gatgacaatc gaaatttaag taaactatcc agcaaatctt
    28201 tctattataa aaggcatcct atcaaggttt tcacacgtga ttggatgcct tttttctgat
    28261 gactagattt tttgtattac caaataatca cgcgctcctc tggtgaacgc cacattccgt
    28321 ctccttcttt gacatcatag gttgtaaaga aatcgtcgaa gtttggtact tgcacattga
    28381 cacggagttt ggctggtgcg tgcacatcga cgctagccaa aagtttcata aattctggac
    28441 gacctttcat gcgccagatg cgaccgaagt tgtagaagaa ctcttctgct gagaagtctg
    28501 cttctctctt agctgcttca agcgctgctg cgattcctcc caagtcagcc acgttttctg
    28561 atacagtcaa tttaccgtta atggttgctc cataagaatc ctgtccatca aattggtcaa
    28621 tgactttttg tgttttctcc ttgaaggcag catagtcgct ctctgtccac caatccttga
    28681 ggctaccatt ttcgtcaaag gaagccccgt tagtatcaaa ggcgtgggaa atttcatggg
    28741 caatcactgc cccaatacca ccgtagttag cagaagatga ctgatgcaag tcatagaaag
    28801 gcgcctgtaa aatggccgct ggaaagacaa tcaggttctt ctgaggattg tagtaggcat
    28861 tgaccatatg agcaggcatg ccccattcct tatagtctac aggctggttc cacttactcc
    28921 aactgtgctt gatttccaca cgcgcaaagg ctagagcatt ctcaaaaaga ctggcagttt
    28981 cattcactac cttatccttg taacgtgcag gcaattcttc tggatagcca atataaggtt
    29041 tgatcacatt gagcttcacg atagcctgtt tacaggtttc tggagtgagc cagtcattct
    29101 taagcagacg ctccttatag acatcaatca tggttgccac ttttttctcc acatccgcct
    29161 tggcttctgg agagaacttc tcacgggcgt accaaagccc cagggcttgc ttgaaaggtt
    29221 cctgtgctag atgataagct gctttgacct tatcttttgc ctctggaact ccagaaaggg
    29281 cacggctgta ggcaccagac aaaacacgga tatcctctgt taaatagctg gttgaaagat
    29341 tgacaacact caaaatcaag gttgctttaa ggagagacca ggcttcctca ctgtagaatt
    29401 gctctgctgc ttgccagaaa cgttcctcgt ctacaataac cttgtctggt aattgcccaa
    29461 taactgcttt gaagaagtca tccaaaggta gggcaggcgc gaatttcttg aaatcttcgt
    29521 aagaatatgg atgatagagt ttagcatatt ctgaactttc ttcattagag agcaccactg
    29581 tcgcaactcg gcggtccaat tcaagtcttt tttctagcaa gtcttcaatt tcttcatcag
    29641 agaaatcata agccttgagg agatttgcgc tgctttcttt ccaaagagtc aagagctctt
    29701 cgcgctgagg atgttcttct gcatagtagg tcgtatctgg caagattgtg cttggagcgc
    29761 tagcccatag aacattgatt ctagcatcca taaagtctgg cgatacacca aaaggaagga
    29821 agtttggttt tcctgcaagc tcaaactctg ctagtttagc tgtaaaatcc gcaaaagttt
    29881 ccaattcttg gaattcttta aggagtggta agacaggtgt gataccgtca gcttctctct
    29941 tgtcaaaatc acgaactagg cggtggtatt tgacaaagtt ttccaagata gcatcctcag
    30001 gcacttcttc acctgctaac cacttgtctg ttgtcgccag catcaggtct tcaatttcct
    30061 ggtctaaatc aacaaaacct ccagtttgag acttatctgc tgggatttca gctgtctgtt
    30121 gccattctcc attgatagca tcataaaaat catcttgata acgtgtcatc ttgttctcgc
    30181 tttcatttgt atttgcattt atcttaacaa aaatcactag ggaatgcaat taat
//
LOCUS       DAVKAO010000019        29586 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKAO010000019 DAVKAO010000000
VERSION     DAVKAO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29586
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.019"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(56..1066)
                     /locus_tag="VWX16_000879"
     CDS             complement(56..1066)
                     /locus_tag="VWX16_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9158260.1"
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VWX16_000880"
     CDS             1215..2384
                     /locus_tag="VWX16_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9158261.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VWX16_000881"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VWX16_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9158262.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VWX16_000882"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VWX16_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9158263.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETEVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VWX16_000883"
     CDS             4146..4379
                     /locus_tag="VWX16_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9158264.1"
                     /translation="MKEKEIFDSIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            4425..4716
                     /locus_tag="VWX16_000884"
                     /pseudo
     CDS             4425..4716
                     /locus_tag="VWX16_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4919..5149
                     /gene="blpU"
                     /locus_tag="VWX16_000885"
                     /pseudo
     CDS             4919..5149
                     /gene="blpU"
                     /locus_tag="VWX16_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
     gene            5864..8017
                     /locus_tag="VWX16_000886"
     CDS             5864..8017
                     /locus_tag="VWX16_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9158265.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGEMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELVEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            8030..9379
                     /locus_tag="VWX16_000887"
     CDS             8030..9379
                     /locus_tag="VWX16_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9158266.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            9549..10256
                     /locus_tag="VWX16_000888"
     CDS             9549..10256
                     /locus_tag="VWX16_000888"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9158267.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            10458..14183
                     /locus_tag="VWX16_000889"
     CDS             10458..14183
                     /locus_tag="VWX16_000889"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9158268.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISEIRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDGKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            14276..15718
                     /locus_tag="VWX16_000890"
     CDS             14276..15718
                     /locus_tag="VWX16_000890"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9158269.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            15755..16777
                     /locus_tag="VWX16_000891"
     CDS             15755..16777
                     /locus_tag="VWX16_000891"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9158270.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGAIGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            16774..17319
                     /gene="purN"
                     /locus_tag="VWX16_000892"
     CDS             16774..17319
                     /gene="purN"
                     /locus_tag="VWX16_000892"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9158271.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            17403..17912
                     /locus_tag="VWX16_000893"
     CDS             17403..17912
                     /locus_tag="VWX16_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9158272.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            17937..19484
                     /gene="purH"
                     /locus_tag="VWX16_000894"
     CDS             17937..19484
                     /gene="purH"
                     /locus_tag="VWX16_000894"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9158273.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAANKYGLTMVFT
                     GVRHFRH"
     gene            19606..20868
                     /gene="purD"
                     /locus_tag="VWX16_000895"
     CDS             19606..20868
                     /gene="purD"
                     /locus_tag="VWX16_000895"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9158274.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRTAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTVATVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKASK"
     gene            21271..21759
                     /gene="purE"
                     /locus_tag="VWX16_000896"
     CDS             21271..21759
                     /gene="purE"
                     /locus_tag="VWX16_000896"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9158275.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRILSVEDKSIADALANFAEEQGKITEESSNE
                     LI"
     gene            21746..22846
                     /gene="purK"
                     /locus_tag="VWX16_000897"
     CDS             21746..22846
                     /gene="purK"
                     /locus_tag="VWX16_000897"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9158276.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEVD
                     LEAAYALADSADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTI
                     VPARISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSI
                     EACDFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYG
                     KIEAKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            22856..23083
                     /locus_tag="VWX16_000898"
     CDS             22856..23083
                     /locus_tag="VWX16_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9158277.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            23146..24444
                     /gene="purB"
                     /locus_tag="VWX16_000899"
     CDS             23146..24444
                     /gene="purB"
                     /locus_tag="VWX16_000899"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9158278.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(24499..28425)
                     /gene="strH"
                     /locus_tag="VWX16_000900"
     CDS             complement(24499..28425)
                     /gene="strH"
                     /locus_tag="VWX16_000900"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9158279.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPATEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKKIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDIL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEILSQPAPAEESK
                     ALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(28741..29457)
                     /locus_tag="VWX16_000901"
     CDS             complement(28741..29457)
                     /locus_tag="VWX16_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9158280.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT         8229 a         5772 c         7116 g         8469 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtattaga
       61 aaatgccgat aagggtctgc ataccaaggc tggtgaggat gatggcaatc cagcagacgg
      121 ctccgagaac aatggatttt ccactggatt tgaccatagc gaccagatta gttttgagac
      181 cgatggcact catggccatg ataatgagga atttagagag ttgtttgaga ggggtaaaga
      241 aactactaga cacaccgaga gaggtcagaa gggtggttag gagcgatgca aggatgaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaagcc ttgcttattt ttttgctcgc
      361 gactttgcca gtaggagaga aagagagtga tggggatgat agctagggtg cgcgtgagtt
      421 tgacaatggt tgcggattcg agggtattgg tctggtagag actgtcccaa gcgctagctg
      481 tggcggttac agaggaagta tcattgaccg cagttcctgc aaagagggcg aagccgtcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggagatggct tgggctactt ccttttcctt agcatggata acaggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaatcaag gtagccagtt
      721 ttgtatccag ggcaaagaag cgctggaaga ggtaggcaac aatcaaggct attgaaatgg
      781 tggaaaggat gacagggagt gaagattgcc caactgcgaa gacttgagag atattgagac
      841 caaaaccaag taagataacg gcatactgga gcaatttctt ggaactaaag gtcaatccaa
      901 catccagttg tttataagac gagagaaagg gatgtaggag catgcccata aaaatcgcaa
      961 aaacgggtgc gccaataaca gggaagaatc ctcctaagta ccaagatagg atggaaatga
     1021 gaaggcagac caagatgcct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 agaatctgtt accattatag acctgtaaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcgggaggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggcta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcgg
     1441 ccagtgattt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaatcttgg
     1501 tcactatcgg agcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtgct cttgccagct cctgcttatc cgggttatga accgatcgtc aatctagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacgggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat ttttgctcct gcgaccttca
     1981 cagcccagtt aatcaagagt caccagtact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaacgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgtctct gtccagagca ttatcatgag gatgagagac gttgccatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaactata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat cgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactctacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attcggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgctttgt tggctgctgg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggacttatgt caacattgcc gactgtagat
     3541 gggaaaggtt ttgacatgct tgacctcggt gctaatgcag aaaatacagc ccagcacctc
     3601 catcaatacg ctgtcctagg ttctttctat gccaagaatg ttcgtggcat tgcgcaacca
     3661 cgtgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt caaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgccagatt
     4081 cgtaccatgc tagaaacaga agtagttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgaa agaaaaagaa atttttgaca gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgacttggat gctgactcag
     4261 ttgatttgat ggagtttatc ttgacgctgg aggatgaatt tagtatcgaa atcagcgatg
     4321 aggaaattga ccaactccaa agtgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aatgagaaat atcggacaag
     4441 ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat cctcaagcac
     4501 aaacttccac gtaaatccag caaactcaag ccgctaacag ttgaagataa agcctgtaat
     4561 catgcgctat ctaaggagag aagcaaggtt gagaatatct ttgccaaagt aaaaacgttt
     4621 aaaatgtttt caacaaccta tcgaaatcat cgtaaacgct tcggattacg aatgaatttg
     4681 attgctggta ttatcaatca tgaactagga ttctagtttt gcaggaagtc taatagtaaa
     4741 aaagtgatta gaaaacatct tttttaaaaa tagagatgat tttgaaacaa aaaagctaat
     4801 tcaagacgtt tcgatgccaa ttcaagattt ggatgaaaaa aattaataga tactgttata
     4861 ctaaacttgt caagtttgta acaagacaaa tattaaaaat aaaaaagagg tattcgttat
     4921 gaatacaaaa aagatgtcac aatttgaaat tatggatact gagatgcttg cttgagttga
     4981 aggtggcgga tgcaattggg gagattttgc caaagcaggt gttggaggag gagtagcacg
     5041 aggtcttcag ctaggaatta aaacaagaac atggcaaggt gcagcaactg gtgctgcggg
     5101 aggagctata cttggaggtg tggcctatga agcgacatgt tggtggtaat tatggatttt
     5161 aaaagtttta ttattggttt agtagttggt atatttggtc cttatatgga tgatttaatt
     5221 agaaaaaaat ttttaaagtc ttcggagaag aaaacagaaa aatctgttaa aaaataatca
     5281 aaactataaa tgatgaatct gaatcaaaat tattttgcgc atgtaaagag gagtcttata
     5341 gtaacgagtc aaaaaaggag taactatgaa tcgtaattta gaacggtgtt atctattctg
     5401 actagggata gatcatacca taggtagctt agaaatagca gagacattag aaattgaagt
     5461 aataaatagg atgtcgtaag tgttactatc aatgatttat ttgtttcaag cttgcctagg
     5521 gtgtcagtaa aaaatcattt tcctttcaat agcatatttt tagtgggcag gactcttgtt
     5581 ctgcctattt ttttatccaa aaagtgcagt tgggagggag ataggctcat ttgggaagga
     5641 agtccagttt ttgtttagtg attggggtaa gatagttgtt atcagatgag tttatactct
     5701 tcgaaaatca aattcaaacc acgtcaacgt cgccttgccg tatatatgtg actgacttcg
     5761 tcagtcctat ctacaacctc aaaacagtgt tttgagcagc ctgcggctag tttcctagtt
     5821 tgctctttga ttttcattga gtattaggga aaaggagatg aatatgaaat ttgggaaacg
     5881 tcactatcgt ccgcaagtgg atcagatgga ctgcggtgta gcttcattag ccatggtttt
     5941 tggctactat ggtagttatt attttttggc tcacttgcga gaattggcta agacgaccat
     6001 ggatgggacg acggctttgg gcttggtcaa ggtggcagag gagattggtt ttgagacgcg
     6061 agccattaag gcggatatga cgctttttga cttgccggat ttgacttttc cttttgttgc
     6121 ccatgtgctt aaggaaggga aattgctcca ctactatgtg gtgactgggc aggataagga
     6181 tagcattcat attgccgatc cagatcccgg ggtgaagttg actaaactgc cacgtgagcg
     6241 ttttgaggaa gaatggacag gagtgactct ttttatggca cctagtccag actataagcc
     6301 ttataaggaa caaaaaaatg gtctgctctc ttttatccct atattagtga agcagcgtgg
     6361 cttgattgcc aatatcgttt tggcaacact cttggtaacc gtgattaaca ttgtgggttc
     6421 ttattatctg cagtctatca ttgataccta tgtgccagat cagatgcgtt cgacactagg
     6481 gattatttct attgggctag tcatcgtcta catcctccag caaatcttgt cttacgctca
     6541 ggagtatctc ttgcttgttt tggggcaacg cttgtcgatt gacgtgattt tgtcctatat
     6601 caagcatgtt tttcacctcc ctatgtcctt tttcgcgaca cgcaggacag gggaaattgt
     6661 gtctcgtttc acggatgcta acagtattat cgatgcgctg gcttcgacca ttctttcgat
     6721 tttcctagat gtgtcaacag ttgtcattat ttcccttgtt ttattttcac aaaataccaa
     6781 tctctttttc atgactttat tggcgcttcc tatctacaca gtgattatct ttgcctttat
     6841 gaagccgttt gaaaagatga atcgggacac catggaagcc aatgcggttc tgtcttcttc
     6901 tatcattgag gacatcaacg gtattgagac tatcaagtcc ttgaccagtg aaagtcagcg
     6961 ttaccaaaaa attgacaagg aatttgtgga ttatctgaag aaatccttta cctatagtcg
     7021 agcagagagt cagcaaaagg ctctgaaaaa ggttgcccat ctcttactta atgtcggcat
     7081 tctctggatg ggggctgttc tggtcatgga tggcaagatg agtttggggc agttgattac
     7141 ctataatacc ttgctggttt actttaccaa tcctttggaa aatatcatca atctgcaaac
     7201 caagcttcag acagcgcagg ttgccaataa ccgtctaaat gaagtgtatc tagtagcttc
     7261 tgagtttgag gagaagaaaa cagttgagga tttgagcttg atgaagggag agatgacttt
     7321 caagcaggtt cattacaagt atggctatgg tcgagacgtc ttgtcggata tcaatttaac
     7381 cgttccccaa gggtctaagg tggcttttgt ggggatttca gggtcaggta agacgacttt
     7441 ggccaagatg atggttaatt tttacgaccc aagtcaaggg gagattagtc tgggtggtgt
     7501 caatctcaat cagattgata aaaaagccct acgccagtac atcaactatc tgcctcaaca
     7561 gccctatgtc tttaacggaa cgattttgga gaatcttctt ttgggagcca aggaggggac
     7621 gacacaggaa gatatcttac gggcggtcga attggtagag attcgagagg atatcgagcg
     7681 catgccactg aattaccaga cagaattgac ttcggatggg gcagggattt caggtggtca
     7741 acgtcagaga atcgctttgg cgcgtgctct cttgacagat gcgccggtct tgattttgga
     7801 tgaggcgact agcagtttgg atattttgac agagaagcgg attgtcgata atctcatggc
     7861 tttggacaag accttgattt tcattgctca ccgcttgact attgctgagc ggacagagaa
     7921 ggtggttgtc ttggatcagg gcaagattgt cgaagaagga aagcatgctg atttgcttgc
     7981 acagggtggc ttttacgccc atttggtcaa tagctagaaa gaggagagga tgaaaccaga
     8041 atttttagaa agtgcggagt tttataatcg tcgttaccat aatttttcca gtagtgtgat
     8101 tgtacccatg gcccttctgc tcgtgttttt acttggcttt gcaactgttg cagaaaagga
     8161 gatgagtttg tccactagag ctactgtcga acctagtcgt atccttgcaa atatccagtc
     8221 aactagcaac aatcgtattc ttgtcaatca tttggaagaa aataagctgg ttaagaaggg
     8281 ggatcttttg gttcaatacc aagaaggggc agagggtgtc caagcggagt cctatgccag
     8341 tcagttggac atgctaaagg atcaaaaaaa gcaattggag tatctgcaaa agagcctgca
     8401 agaaggggag aaccactttc cagaggagga taagtttggc taccaagcca cctttcgcga
     8461 ctacatcagt caagcaggca gtcttagggc tagtacatcg caacaaaatg agaccatcgc
     8521 gtcccagaat gcagcagcta gccaaaccca agccgaaatc ggcaacctca tcagtcaaac
     8581 agaggctaaa attcgcgatt accagacagc taagtcagct atggaaacag gtgcttcctt
     8641 ggccagtcag aatctagcct actctcttta ccagtcctac aagtctcagg gcgaggaaaa
     8701 tccccaaact aaggttcagg cagttgcaca ggttgaagca cagatttctc agttagaatc
     8761 tagtcttgct acttaccgtg tccagtatgc aggttcaggt acccagcaag cctatgcgtc
     8821 agggttaagc agtcaattgg aatcccttaa atcccaacat ttggcaaagg ttggtcagga
     8881 attgaccctt ctagcccaga aaatcttgga ggcagagtca ggtaagaagg tacagggaaa
     8941 tcttttagac aaggggaaaa ttacggcgag tgaggatggg gtgcttcatc ttaatcctga
     9001 gaccagtgat tctagcatgg ttgcagaagg tgccctacta gcccaacttt atccatcttt
     9061 ggaaagagaa gggaaagcca aactcacagc ctatctaagt tcaaaagatg tagcaagaat
     9121 caaggtcggt gattctgttc gctatactac gactcatgat gccgggaatc aacttttcct
     9181 agattcaact attacaagta ttgatgcgac agctactaag actgagaaag ggaatttctt
     9241 taaaatcgag gcggagacta atctaacttc ggagcaggct gaaaaactta ggtacggggt
     9301 ggaaggccgc ttgcagatga ttacgggcaa gaaaagttat ctacgttatt atttggatca
     9361 atttttgaac aaagagtaat gttcgtgttt ttagagttaa ataattttta aactgtgaga
     9421 aagattcttc ttgcagtttt ttctttacaa tttttgaaaa acatctacta tttattcggt
     9481 taaattcttg tgttttttgg ttttttgtgg taaaatgtgc tcaagtaata cgaaaggcga
     9541 actttaaaat gtcaaaacaa ttgatctatt cgggaaaagc taaagatatc tatacaactg
     9601 aggatgaaaa tcttattatt tcaacttaca aggaccaggc gactgctttc aacggtgtca
     9661 agaaggagca gattgcaggt aagggagtct tgaataatca gatctcatct tttatttttg
     9721 agaaattaaa tgcggctggt gtggcgactc actttgtgga gaaactttca gacacggaac
     9781 aactcaataa aaaggttaag attattcctt tggaagtcgt gctccgcaac tatactgctg
     9841 gttccttttc aaaacgtttt ggtgtggatg agggaatcgc cttggagact ccgattgtcg
     9901 aattttacta caaaaatgat gatttggatg atccatttat caatgatgag catgtgaaat
     9961 tcctacagat tgcgggtgac cagcagattg cctacttgaa ggaagaaacg cgtcgtatca
    10021 atgaactatt gaaagtctgg tttgctgaga ttgggcttaa attgattgac tttaagctag
    10081 agttcggttt tgacaaggat ggcaagatta tcttggcaga cgaattttca ccagataact
    10141 gccgcttgtg ggacgctgat ggcaaccaca tggataagga tgttttccgt agaggattgg
    10201 gagaactaac cgacgtttat gagattgttt gggaaaagtt gcaggaattg aaataatctg
    10261 tttgcaacgg aaaaccttcg tctctcaact aaaaggactc aggctgaaaa ggtcccccag
    10321 accttttcac tctgtagaga actaggtgaa ctaacagatg tttacgaaat tgtctgggaa
    10381 aagttgcagg gtttaaaata acaacctcaa ggctgtttgg gaatattgca agagctgaaa
    10441 taaaggaata agaattgatg gataaacgta tttttgttga aaaaaaggct gattttcagg
    10501 tcaagtcaga gagtttggtt agagagctcc agcacaactt gggactgtca agcttgaaaa
    10561 gtattcgtat tgtgcaagta tatgatgtat ttgacttggc tgaggacttg tttgcacctg
    10621 cagagaagca cattttctct gagcagggaa ccgaccatgt tttagatgaa gtatctgtgc
    10681 aggcggatct tgctaactat gctttctttg ccattgaaag tctgccaggg cagtttgacc
    10741 agcgtgcagc ttcgtcacag gaagccttgc ttttgttggg aagttcgagt gacgtgacag
    10801 tcaacacagc ccaactttac ttggtgaata aagatattga tgcgactgag ttggaagctg
    10861 tcaaaaacta cctgctcaat ccagttgatt ctcgtttcaa ggatatcacg acagggattg
    10921 ccaagcagga gttttcagag tcagacaaga ccattccaaa attgactttc tttgaaagct
    10981 atacagcaga agactttgct cgctacaagg ccgagcaagg gatggccatg gaagtggatg
    11041 atttgctctt tatccaagac tactttaagt caatcgggcg cgtgccaact gagactgaac
    11101 tcaaggtttt ggacacttac tggtctgacc actgccgtca tacgactttt gagacagagt
    11161 tgaaaaacat cgacttttca gcttctaaat ttcaaaagca attgcagtca acctatgaca
    11221 agtatattgc catgcgcgag gaattaggtc ggtctgaaaa accacaaacc ttgatggata
    11281 tggcgactat tttcggtcgt tatgagcgtg ctaatggacg attggatgat atggaagtct
    11341 ctgacgaaat caatgcctgc tcagttgaaa ttgaagtgga cgttgatggt gtcaaggaac
    11401 cttggctcct catgtttaaa aatgaaaccc acaaccatcc aacagaaatt gagccatttg
    11461 gtggagcggc tacctgtatc ggtggagcta ttcgtgatcc gttgtcaggc cgttcctatg
    11521 tttaccaagc catgcgtatt tcaggtgctg gtgatattac agcaccgatt tcggaaattc
    11581 gtgctgggaa attgccacaa caagtcattt ctaaaacagc agctcatggt tattcttcat
    11641 atggtaacca gattgggctt gccacaacct acgttcgtga atacttccac ccaggctttg
    11701 tagctaaacg tatggaactt ggtgccgttg ttggtgcggc tcccaagggc aatgttgtcc
    11761 gtgaaaaacc tgaagcaggt gatgtgatca tccttctcgg aggcaaaaca ggtcgtgatg
    11821 gtgtcggtgg tgcgacgggc tcttctaagg ttcaaacagt tgagtctgta gagactgctg
    11881 gtgctgaggt tcaaaaagga aatgccatcg aagaacgcaa gattcagcgc ctcttccgta
    11941 atggcaatgt cactcgtctg atcaagaagt ccaatgactt tggggcaggc ggcgtctgtg
    12001 tggctattgg tgaattggca gacggtcttg aaatcgacct caacaaggtg cctcttaaat
    12061 accagggctt gaatggtaca gaaattgcca tctctgaatc acaagaacgg atggcggtcg
    12121 tggttcgtcc tgaagatgtg gatgccttcg ttgccgaatg taacaaagaa aatattgatg
    12181 ctgttgtggt ggcgacagta actgaaaaac caaatcttgt catgcactgg aatggtgaga
    12241 caatcgttga cttggagcgt cgtttccttg acaccaatgg tgtgcgcgtg gttgtcgatg
    12301 ccaaagttgt ggacaaggat ggcaaactcc cagaagagcg tcaaacatct gctgaaacac
    12361 tggaatcaga tacccttacg gttctatctg acctcaacca tgcaagtcaa aaaggattac
    12421 agactgtctt tgactgctct gttggacgct caacggttaa tcacccactt ggtggtcgtt
    12481 accaactcac accaactgag gcatctgtgc agaaattgcc agttcaacac ggtgtgactc
    12541 atactgcgtc ggtcattgct caaggtttca acccatatgt agctgaatgg tctccatacc
    12601 acggtgctgc ttacgcggtt atcgaagcaa ctgctcgttt ggtggctgct ggtgccaact
    12661 ggtctaaggc tcgtttctct taccaagaat atttcgagcg gatggacaag caagcagagc
    12721 gttttggtca gccagtagcc gcccttctag gctctattga agctcagatt caacttggct
    12781 tgccatctat cggtggtaag gactccatgt ctggtacctt tgaagaattg accgttccgc
    12841 caaccttggt tgcctttggg gtgacgacgg cagatagccg taaggtgctc tctccagaat
    12901 ttaaagctgt tggggaaaat atctactaca tcccaggtca agccctctct gcagagattg
    12961 attttgactt gattaagaaa aattttgctc agtttgaagc aagccaagct gaccataaag
    13021 tgacatctgc atcagctgtc aaatacggtg gtgtagttga aagtttggct cttgctacct
    13081 ttggaaacta tattggtgca gaggtgacct tgcctgaact taaaacagct ttgacagctc
    13141 aattaggcgg ctttgtcttc acatctcctg aagaaattgc tggagtagag aagattggac
    13201 aaacgaaagc agactttaca ctgactgtca acggtgtgaa gctagatgga cacaagcttg
    13261 acagtgcatt tcaagggaca ttggaagaag tttacccaac agaatttacc caagcgaaag
    13321 aactagaaga agtaccagct gtggcatcag atgttgtgat taaagccaaa gaaaaggttg
    13381 aaaaacctgt ggtttacatc ccagtctttc caggaaccaa ctcagaatat gattcagcta
    13441 aggccttcga aaaagaaggt gcagaggtca atttggtgcc attcgtgacc ttgaatgaag
    13501 aagctattgt caagtcagtt gaaactatgg ttgacaatat cgacaagact aatattctct
    13561 tctttgccgg tggattctcg gctgcggatg aaccagatgg ttcagctaag tttatcgtca
    13621 atatcctgct taatgaaaaa gtgcgtgtgg ctattgatag ctttatcgcc cgtggtggct
    13681 tgattatcgg tatttgtaat ggattccaag ccttagtcaa atcgggtctc ctaccatacg
    13741 gaaactttga agatgctaac agtactagtc caaccctctt ctacaatgat gccaaccaac
    13801 acgtggccaa gatggtggaa actcgcattg ccaataccaa ctcaccatgg ttggctggtg
    13861 tgcaagtggg cgatatccac gctattcctg tttcgcacgg tgaagggaag tttgtcgtga
    13921 cggctgagga atttgcagag ctccgtgaca atggacaaat tttcagccaa tacgttgact
    13981 ttaacggtaa accaagtatg gattctaagt acaatccgaa tggttctgtc catgccatcg
    14041 aaggaattac cagcaagaat ggtcaaatca tcggtaagat gggccactca gaacgttatg
    14101 aggatggtct tttccaaaat atcccaggca ataaagacca acacctgttc gcatcagcgg
    14161 ttaaacattt cactggaaaa taagacttac agattttcta atagatagta tcagtaatgt
    14221 aaaagtcatg taaatctagc tcttgatgat tacaaatgaa aattaggtat aaaaaatgac
    14281 atacgaagta aaatctctta atgaagaatg tggtgttttc ggtatttggg gacatccaga
    14341 tgctgctaag ttgacctatt ttggactcca cagtcttcaa caccgtggtc aggagggggc
    14401 aggaatcctc tccaacgatc aaggacaact gaagcgccat cgtgacatgg ggcttttatc
    14461 agaagttttc agaaatccag ctaatttgga taaattgaca ggagctggtg cgattgggca
    14521 tgtgcgttat gcgactgctg gcgaagcttc tgtagataac atccagccct tcctcttccg
    14581 ttttcacgat atgcagtttg gcttggctca taatggaaat ctgaccaatg cagcctctct
    14641 caagaaagaa ctggaacaaa gaggagcaat tttcagcgcg acttcggact ctgaaatctt
    14701 ggctcacctc attcgtcgca gtcataatcc tagcctgatg ggcaaaatca aggaagcgct
    14761 cagccttgtc aaaggtggtt ttgcctatat cttgctgttt gaggacaagt tgattgcggc
    14821 tcttgaccct aatggctttc gtccgctttc tatcgggaaa atggccaacg gagcggtggt
    14881 tgtttcctct gaaacctgtg cttttgaggt cattggtgcc gagtggattc gtgatttgaa
    14941 gccaggtgag attgtgatca ttgatgacga gggcattcag tatgacagct atacagatga
    15001 tacccagttg gcgatttgtt ctatggagta tatctacttt gcccgccctg attctaatat
    15061 ccacggtgtc aatgtccata cggcacgtaa gagaatggga gcgcaattgg cgcgagaatt
    15121 taagcatgag gcagatattg tagttggtgt gcccaattct tccctaagcg cggctatggg
    15181 atttgcggaa gaatcaggct taccaaatga aatgggtctg atcaaaaacc aatacaccca
    15241 gcgaactttt atccaaccga ctcaagaatt gcgggagcaa ggagtgcgga tgaaactgtc
    15301 tgctgtttcg ggtgttgtca aaggcaaacg tgtggtcatg gtggatgatt ccattgtacg
    15361 tggaacaacc tctcgtcgta tcgttcagct cttgaaagaa gcgggtgcga ctgaggttca
    15421 cgttgccatt ggaagtcctg cactagcgta tccatgtttc tacgggattg atatccagac
    15481 ccgtcaggag ctgattgcag ccaatcatac ggtcgaagaa actcgccaaa tcattggtgc
    15541 ggacagtctg acttatcttt caattgatag cttgattgag tcgattggta tcgaaacaga
    15601 tgcgccgaac ggtggtctct gtgtcgctta ctttgacggt gactacccaa cgcctctcta
    15661 tgactacgaa gaagactatc gtagaagttt ggaagaaaag accagttttt acaagtaggc
    15721 gacagattct ccattaaaga aaaggaaaaa acaaatgaca aataaaaatg catatgccca
    15781 gtcgggtgtg gatgttgaag cgggttatga agttgttgaa cggattaaaa agcacgtggc
    15841 ccgtacggag cgtgcaggtg tcatgggagc tattggtggc tttggtggta tgtttgacct
    15901 ttccaagact ggggttaaag aacccgtctt gatttcaggg actgacggtg tcggaaccaa
    15961 gctcatgttg gctatcaagt acgacaagca cgataccatc gggcaggact gtgtggccat
    16021 gtgtgtcaac gacatcattg ctgcaggtgc ggaacccctc tattttctcg actacgtagc
    16081 gacagggaag aatgaaccag ctaagctaga acaagtggtt gctggtgtgg cagaaggttg
    16141 tgtgcaggct ggtgctgccc tcatcggtgg ggaaacggct gaaatgccgg gcatgtacgg
    16201 cgaagacgac tatgacttgg ctggttttgc ggtcggtgtg gctgaaaaat ctcaaatcat
    16261 tgacggttca aaggtggtag agggagatgt tcttctcgga cttgcttcaa gtgggattca
    16321 ctcaaatggt tactctttgg ttcgtcgtgt ctttgcggat tacacaggtg aggaagtcct
    16381 accagaattg gaaggcaaga aacttaagga agttctactt gagccgactc gtatctatgt
    16441 caaggctgtc ttgccgctca tcaaagaaga gttggtcaac ggcattgccc acatcacagg
    16501 tggtggcttt atcgaaaatg tccctcgtat gtttgcagat gacctagctg ctgaaattga
    16561 tgaaagcaaa gttccagtgc ttccaatttt caaagccctt gaaaaatatg gtcagattaa
    16621 acacgaagaa atgtttgaaa tcttcaatat gggtgtggga cttatgttgg cggtcagccc
    16681 tgaaaatgta gagcgtgtaa aagaattgtt ggatgaagca gtctatgaaa ttggtcgcat
    16741 cgtcaagaaa gaaaacgaaa gtgtcattat caaatgaaaa aaatagcggt ttttgcctct
    16801 ggtaatggct caaattttca ggtgattgcc gaagaatttc cagtggagtt tgtcttttca
    16861 gaccatcgtg atgcctatgt gcttgagcgt gcaaagcagc tcggcgttct gtcctatgct
    16921 tttgaactca aggagtttga gagcaagaca gactacgaag cagcccttgt cgaactcttg
    16981 gaagaacacc agattgactt ggtttgccta gcaggctaca tgaaaatcgt tggaccaacc
    17041 ttattgtcgg cttatgaagg tcggattgtc aacattcatc cagcctactt gccagaattt
    17101 ccaggagctc atgggattga ggatgcttgg aatgctggcg tgggtcagtc tggtgtgacc
    17161 attcactggg tggattcggg tgtggataca ggccaggtca tcaaacaggt tcgtgtgcca
    17221 cgactagctg atgataccat tgacagattt gaagctcgca tccatgaagc agagtacagg
    17281 ttgtatccgg aagtagtgaa ggctctattt acagattgac tttttgatga ttcatatgat
    17341 atctttgatt ttaaattgga gtcagtgttt gttgaagacg gcttcaaacg gaggtatttg
    17401 taatgttaga atctaaaaaa acaactcgat atgtatttta tgtctatctg atgttattaa
    17461 cttggggaat cttatttaag tttgaaacaa atcctgaatt tatagcattt ttcttagctc
    17521 caaggtatat caattggatt ccattttcag aaccactaat agtcgatgga aaaattgttt
    17581 ttgctgaaat gttatttaat ctgatttcct ttattccatt aggtgtttgt ttccctttga
    17641 taaaaactaa tttatctagt ttaagaatag tcgggacagg tttcttgatt agtttattgt
    17701 ttgagtgctt acagtatatt ttagcaatag gtataacaga tataacggat ttgactttaa
    17761 atacgctagg tgtctgtgta ggcttactga tttatcaaat ttttataaga gtgttcaaat
    17821 cacagactag aaaatggatc aatatcttag gtatgcttag ccttggtttt gcttatcttg
    17881 ttttactgtt actgcattta attggtgttt aactaatgat taaaaaggag aatataatga
    17941 ctaaacgcgt cttaatcagc gtctcagaca aagcgggcat tgttgaattt gcccaagaac
    18001 tcaaaaaact tggttgggag attatctcaa caggtggaac taaggttgcc cttgataatg
    18061 ctggggtgga taccattgct atcgatgatg tgactggttt cccagaaatg atggacggtc
    18121 gtgtgaagac cctccaccca aatatccacg gagggcttct cgctcgtcgt gacttggata
    18181 gccacttgga agcggctaag gacaacaaga ttgagctcat tgaccttgtg gtggtcaacc
    18241 tttacccatt taaggaaact atccttaaac cagatgtgac ttatgctgat gcagttgaaa
    18301 atatcgatat tggtgggccg tctatgcttc gttcagcagc gaaaaatcat gccagtgtta
    18361 cagttgtggt agatcctgct gactacgctg tggttttgga cgaattggca gcaaacggcg
    18421 aaacctctta tgaaactcgc caacgtttag cagccaaagt atttcgtcac acagcggctt
    18481 atgacgcctt gattgcagaa tacttcacag ctcaagtggg tgaaagcaag cctgaaaaac
    18541 tcactttgac ttatgacctc aagcaaccaa tgcgttacgg tgagaatcct caacaagacg
    18601 cggactttta ccagaaagct ttgcctacag attactctat tgcttcagcc aaacagctca
    18661 acgggaaaga attgtcattt aataatatcc gtgatgcaga tgctgctatc cgtatcatcc
    18721 gtgacttcaa agatagtcca accgttgtgg ctctcaaaca catgaatcca tgtggaattg
    18781 gtcaagctga tgacatcgag actgcttggg actacgctta tgagtctgac ccagtgtcta
    18841 tctttggtgg aattgtcgtc ctcaaccgtg aggtggatgc tgcgacagct gagaagatgc
    18901 acggcgtttt cctcgaaatc atcattgcac caagctatac ggatgaagcg ctagccattt
    18961 tgatcaataa aaagaaaaac ttgcgtatcc ttgccttgcc atttaatgct caagaggcta
    19021 gcgaagtgga agcagaatac acaggtgtag tcggtggact tctcgtgcaa aatcaagacg
    19081 tggtcaagga aagcccagct gactggcaag tggtgaccaa acgtcagcca accgagacag
    19141 aagcgactgc tcttgagttc gcttggaagg ctatcaagta cgtcaaatca aatggtatta
    19201 tcgtgaccaa cgaccacatg acacttggtg ttggtccagg tcaaaccaac cgtgtggctt
    19261 ctgttcgcct tgccattgac caagccaaag atcgtctgga cggggcggtc cttgcttcag
    19321 atgccttctt cccatttgcg gataacgtgg aagaaatcgc caaagcagga attaaggcca
    19381 tcatccagcc cggtggctct gtccgtgacc aagaatccat cgaagcagcg aataaatacg
    19441 gcttgactat ggtctttaca ggtgtgagac attttagaca ttaagaagat aaaagggaag
    19501 aaaacagttt ctttcctttt ttggcttaaa atgctaactg aaacaagatt aaaacgaact
    19561 tttttgatat aatgttggta aataattcgc aaaagaggtt gaggaatgaa actgcttgtt
    19621 gtcggttctg gtggtcgtga gcatgcgatt gctaaaaagt tacttgaatc aaaagacgtg
    19681 gaaaaagtct ttgtagctcc tgggaatgat gggatgactc tggatggttt ggaattggta
    19741 aatatctcta tttccgaaca ttctaaattg attgacttcg caaagaccaa tgatgttgct
    19801 tggaccttta tcggtccaga tgatgccctt gctgctggta tcgtggatga ttttaaccaa
    19861 gctgggctta aggcctttgg tccgactagg actgcagcgg agctggagtg gtccaaggat
    19921 tttgccaagg aaatcatggt caaatatggt gtttcgacag cagcctatgg cacattttca
    19981 gatttcgagg aagcaaaggc ctatatcgaa aagcatggtg cgcctatcgt agtcaaggcg
    20041 gatggcttgg cgcttgggaa gggtgtcgtc gttgctgaga cggttgagca agcggtcgaa
    20101 gccgctcatg agatgctttt ggacaataaa tttggtgact caggtgcgcg cgtggttatt
    20161 gaggaattcc ttgaaggaga ggaattttca ctctttgcct ttgttaatgg tgataagttc
    20221 tacatcatgc caacggctca ggaccacaaa cgtgcctacg atggcgacaa agggcctaac
    20281 acgggtggta tgggtgccta tgcgccagtt ccacacttac cacagagtgt agttgataca
    20341 gcggttgaca ccattgtcaa gccagttcta gaagggatga ttaaagaagg tcgcccttat
    20401 ctgggagttc tttacgcagg gcttatcctg acagctgatg gaccgaaagt cattgagttc
    20461 aacgctcggt tcggagatcc agaaactcag attatcttgc ctcgcttgac ctctgacttt
    20521 gctcaaaata tcacagatat cctggatagc aaggagccaa atatcatgtg gacggacaag
    20581 ggtgtgactc tgggtgtggt tgtcgcatcc aagggctacc cgctagacta tgaaaggggc
    20641 gttgagttgc cagccaagac agaaggcgat gtcatcacct actatgcagg ggctaagttt
    20701 gcggaaaata gcagagcact gctctcaaac ggcggacgag tttatatgct ggttaccaca
    20761 gtagccaccg tcaaagaagc ccaagccagc atctaccaag aactatccca acaaaaaata
    20821 gaaggactct tctaccgaac agatatcgga agcaaggcaa gtaagtaaag atataagaat
    20881 aacgcgacgc agtcgccaaa cacgataatg gtcgtcgtgg tgaaaagacc agaacagtga
    20941 atgttctggt cagggggaaa ctttgagacc ttaggctcaa agtttaggaa tgaaaccgaa
    21001 ggtttgcttc cgcctccatc acctaagacc attatcaaaa agaaaaataa aaattcacaa
    21061 aatacgttaa tggtcgtatg gtttgcgagc gttagcgagc taatatagaa taatcaccgc
    21121 cgttgtgaaa gaacgattgg atgataatcc aatcgttcag ggaaattgga agaccttggg
    21181 tttccaattt aggcatgaga cacctttggt ggctgctgcc gtccctcaca agctaaggtg
    21241 attgttgaaa aagaggaaaa aggagaagaa atgaaaccag taatttccat catcatgggc
    21301 tcaaaatccg actgggcaac catgcaaaaa acagcagaag tcctagaccg cttcggtgta
    21361 gcctacgaaa agaaagttgt ttccgcacac cgtacaccag acctcatgtt caaacatgca
    21421 gaagaagccc gtagtcgtgg catcaagatc atcatcgcag gtgctggtgg cgcagcgcat
    21481 ttgccaggca tggtagctgc caaaacaacc cttccagtca ttggtgtgcc agtcaagtct
    21541 cgtgctctta gtggagtgga ttcactctat tctatcgttc agatgccggg tggggtgcct
    21601 gttgcgacca tggctatcgg tgaagctgga gcgactaacg cagctctctt tgccctccgt
    21661 atcctctctg tagaagataa gtccattgcg gatgcacttg ccaactttgc tgaagaacaa
    21721 ggaaaaatca cagaggagtc gtcaaatgag ctcatctaaa acaatcggaa ttatcggtgg
    21781 cggtcaactg ggtcagatga tggccatttc tgctatctac atgggccaca aggttatcgc
    21841 gctggatcct gcggcggatt gcccggcctc tcgcgtggcg gaaatcattg tggcacctta
    21901 taacgatgtg gacgccctcc gtcagttggc agaccgttgc gatgtcctca cttacgagtt
    21961 tgaaaatgtc gacgctgacg gtttggatgc cgttatcaag gatggacaac tccctcaagg
    22021 aacagatctg ctccgcattt cgcaaaatcg tatttttgaa aaggactttt tgtcaaacaa
    22081 ggctcaagtc actgtggcac cctacaaggt cgtgacttct agcctagact tggcagatat
    22141 cgacttgtcg aaaaactatg tcctcaagac tgcgactggt ggctacgatg gtcatggaca
    22201 aaaggttatt cgttcagaag tagacttgga agcagcctat gcgctagcag actcagcaga
    22261 ctcagcagac tgcgtcttgg aagaatttgt caactttgac cttgagattt ctgtcatcgt
    22321 gtcaggaaat ggcaaggagg tgacgttttt cccagttcag gaaaatatcc accgcaacaa
    22381 tatcctgtct aagaccatcg taccagcccg catttctgaa agtctagtag acaaggctaa
    22441 agctatggca gtgcgaatcg cagaacaact caacttgtct ggaactctct gtgtggaaat
    22501 gtttgcgact gatgatgacg ttattgtcaa tgaaatcgcc ccacgaccac ataactctgg
    22561 gcactattct attgaagcct gtgatttctc tcagtttgac acccatattc tcggtgttct
    22621 cggagcacca ttaccagtca tcaaactcca tgcgccagcc gttatgctta atgtcctcgg
    22681 tcagcatgtc gaggctgctg agcaatatgt gacagaaaat tcaagcgccc acctccacat
    22741 gtatggtaaa atagaagcga agcacaaccg caagatggga catgtgacct tgtttagtga
    22801 tgtaccggat agtgtggatg agcttgggga agggattgat ttttaggaca agtctatgat
    22861 acaaattatc gttaatacat ttattgaaaa ggataagact ggagcagttg ttgaagtgtt
    22921 gtatgccagt gctgaccaag ataaggtaca agctaaatat gaagaactag ctgcacaata
    22981 ccccgaaaat tatttagcta tctatgatgt accgctggat acggatttga atacactaga
    23041 tcattacccg tctgtgttta ttgaaaaaga ggagtttgag tagaattctt ggtttaccta
    23101 gatagcttat tcccaacagc ttaagaagaa aggaaaaatt aacacatgat caaccgttac
    23161 tctcgccctg agatggcgaa tatttggagt gaagaaaata aataccgtgc ttggcttgag
    23221 gtggaaatcc tctctgacga ggcatgggct gagttggggg aaatccctaa ggaagatgtg
    23281 gctttgattc gcaagaaggc ggactttgac atcgaccgta ttttggaaat tgagcaggag
    23341 acgcgccacg atgtggtggc tttcacgcgt gcggtttctg agactcttgg tgaagagcgc
    23401 aagtgggttc actatgggtt aacttctacc gacgtggtgg atactgctta tggttacctc
    23461 tacaagcagg ccaacgacat catccgtcgt gaccttgaaa acttcactaa tatcatcgct
    23521 gacaaggcca aggagcacaa gttcaccatc atgatggggc gtactcatgg tgtgcacgct
    23581 gagccgacaa cctttggtct taaattagca acttggtaca gcgaaatgaa acgcaatatc
    23641 gagcgcttcg agcatgcggc tgctggtgta gaagctggta agatttctgg tgcggttggg
    23701 aactttgcca atatcccacc atttgtagag gagtatgtct gcgataaact tggcatccgt
    23761 gcccaagaaa tctctacaca agtccttcct cgtgaccttc acgctgagta ctttgcggtt
    23821 cttgccagca ttgcgacttc aatcgaacgt atggcgactg agattcgtgg tctacaaaaa
    23881 tctgagcaac gcgaagtaga agagttcttt gctaaagggc aaaaagggtc ttcagcaatg
    23941 cctcacaaac gcaacccaat cggttctgaa aatatgactg gtctggcgcg tgtcattcgt
    24001 ggtcacatga ttacggctta tgaaaacgtc gctctctggc acgaacgcga tatttctcac
    24061 tcatcagctg agcgtatcat cacaccagat acgaccattt tgattgacta catgctcaac
    24121 cgttttggaa atatcgtcaa gaacttgaca gtcttcccag aaaatatgat ccgaaacatg
    24181 aactcgactt ttggtcttat ctttagccaa cgggctatgt tgacattgat tgaaaaaggc
    24241 atgacccgtg agcaagccta tgacttggtg caaccaaaaa cagcctactc ttgggacaac
    24301 caagtagact ttaaaccact tcttgaggca gattcagaag taacatcacg tctcacacaa
    24361 gaagaaatcg atgaaatctt caacccagtt tattacacca aacgagtgga tgatatcttt
    24421 gaacgtcttg gactaggtga ttaattaaaa aataaacagc gagcttcaat ctcgctgttt
    24481 attttttatc gaaaagactt agtcttcttt tcttttagtg agtccatagg ctgctagtgt
    24541 ggccatgagt cctgcgacta ctagtcctgc agaatcgtga gttcctgttt caggaagttt
    24601 tttctctatt accacaggag ctggatcttg aggaagagct ttgctttcct cagcaggagc
    24661 tggttggctt aggatttcta gttttggttt ttcttcagca atagcggctt gtccgttttc
    24721 atcgcctaca tgtgttacca tagttccgac ttcgactatt tgagtaacgg cttcctgtgc
    24781 tacgacacta tttacaagtg ttttcacttc cttaccatcg gcagaagtgc tcacagagta
    24841 gaagttgcta cgatgtccat tgacaccctt agtaatgact tgtgtttttc ctttgagtaa
    24901 gagtggattt tcacgagtca ctgtggtaaa tggaatttct tcttcttgga tatccagtct
    24961 aggttttacc tcagtagttg gtgcaagacc actttcatca cccttgtgag ttacaggagc
    25021 gccaacttca accacttggt ttataacttc tttggttacc tggctatcaa ggactgtttc
    25081 tgttgttttt ccattttcag tgagtacaga gatgtaatga gttcgttcac ctttgactcc
    25141 tgctgtgata atattttcct gaccagctgg gaggttagga ttttctttct tgataacttc
    25201 aaatggaatt tcttcagttc ttgtgatgag ttctggtctg gtttcaacat tggcagccac
    25261 ttcattttca tccaggcttc ctgaatgagt tgcagctggt ttgaggcctt gaagagcggc
    25321 ttttaggttg gctacaagta tgtcaagctc agcttgttta ttacggttga ggttgtaatt
    25381 tagagctgtt ttagctgcgt caagggcctc aagactttct ttactatatc cttctaagtt
    25441 tgtaggaatt ttagctaatt cttcgcggag agcattataa ttagcacgga agtagtcttt
    25501 gttgtggtct gcaaaggcag tcatgagttc aaagatttcc tcttccttgt actcagcgct
    25561 tggtctatct gcccagattg aaagcatact tccgactgtt ggaagatcta cttcaggata
    25621 tttggtagaa gctagttgat tgaatggtgt ttttccagta ttctcaatag ctttcttgag
    25681 gaaaccacca ccatcttctg gtttttgacc aagaatgtag taccagtcac cgttggtatt
    25741 caagaattta tagcctttgc ttgctaggta ttgaggtgat gcgaggttat atccccacca
    25801 gcctttagac cagtaagaaa tcaagacatc tttgtcaaac tgaacatcgt ccttgtcttc
    25861 atagtagaag ccatcgttga aggccattgg ttgaagccct ctttctttgg ccatagctgc
    25921 gagggtgttg gcatattcgg caaatttgcc atagagttga taccacttga ggtagtacca
    25981 gccttgggca ctagtcgcat cgttggcgta ttcgtcagta ccaaagttga aaatctttgt
    26041 tttacctgca aagaagtcca tgtatttacc gatgagggct tttacaaagt tcatcgcttc
    26101 ttcgtttttc aagtccatag ttgtttttga aactttatca aagtgggctt gaggattttt
    26161 aatacctaat ttttccatgg caaccagcat agcatccatg tgacctggac tgttaatagc
    26221 tgggatgaga ccgatgtcct tagatttagc gtattcaatt agctctgtca cttctgcctg
    26281 tgttagtgca gtaccgtttg gatcgtcgta gtaagcttta gttccttcga taatagcttt
    26341 tttaacgtca tcactagcat aggtttttcc gttggcagta atggtcatat catcgagtag
    26401 aaagcgaagt ccgtcatttc ctagaaggag atggacatca gaatatccga gctcactggc
    26461 cttgtctacg atgcgtttga gctggttcag agtaaagtat ttgcgtccag catcgattga
    26521 gattaccttg tttttggcaa gtttttcaac ctcacgttta gcttcttctt ctttttgagc
    26581 ttcaggcgtg agggtcaagt tgttgacagt ttcttgaagt ttagcaatgg cttgatcaat
    26641 cgtatcttgt tgggcacggc taaggttgct atcgagagag cgaatagctt tttcagcttc
    26701 ttttacggcc gtgacgcttt ctgcagtata acggttcagg tcttttggta cctcgttaag
    26761 tgcttgctct gcagattcat aatcagctgc gaagtattca gcgttggcat ttgcaaaatg
    26821 acgcatgagt ttgaagaggc gtgatggtga ataacgtgca gatggagtgt cagcccaagc
    26881 agctaccata ccaccgatga ttgggatatc agctccttct gtttttggta cagaagtgat
    26941 tggtgtgttt ttaataccat tgagcccctg atcgagattg taccagcctt ggccatcagc
    27001 gtttcgtcca agaacgtagt accaagcatc attggtatta aggatttggt gacctttttc
    27061 agctagtagt ttagaagaag cgacatcgta gcctccccaa ccaccagtcc acatagaaac
    27121 gatgatgtct ttgtcaaaac taccaaagct tgtgtcgcta ttgtagtaga taccgtcgtt
    27181 aaaggccatt ggtttgagac cgtgcgattt tacaatacga gcgaggtcat tggcgtaggc
    27241 aataaatttt tcatagcctt ttacagggta gccttcgttt ggatagtatt tatcagcttg
    27301 aagcacactc caacctttag catctgtcgc atcattggca tattcatcaa gtccgatgtt
    27361 gaagatttca gtctttttcg cgaaataagc agcatacttg tcgataaggg cttttgtaaa
    27421 agcgacagct tgttcgttgt caagatcgac agtacgggct gattccttcc caaaatagct
    27481 aaagttaggg ttttggattc ccaattcttt catggcattg agaatcgcat ccatgtgacc
    27541 aggactattt actgtcggaa tgagaccgat acctttatct ttggcatagt taatcagatc
    27601 tgtcatttga ctttctgtta agtgattgcc gtttggatcg ttgtaataat catttgtacc
    27661 tttttcaatg gcgcgtttga catcgtcact ggcataggtc ttgccgttag ctgtgatgct
    27721 catatcgtcc aacatgaaac ggagtccatc atttccgact aataggtgta aatcagtgta
    27781 gccataatgt ttcgctttat cgatgatttt cttgagctgt tctggtgaga aatatttacg
    27841 tccagcatca atagaaacaa ttttcttttt cgctagtttt tcatttacag ttgcagcacg
    27901 ttcctttcct gcctctgttg ccggtttgtc agcctctgct ttcgcttcat cttttttagc
    27961 tggtttatcc ttgtcagtct tgtctgtatt tgactcttta gaatcaacct ctttcgcttc
    28021 ttccttttta gggctagctt cttctgcctt tttattagca gtttcttttt cagcagaagt
    28081 tggagttacc acttctgctt tatcactagg agttgaacta acttcctctt gtggtttttc
    28141 ttctgttttt ggaagactag ctaccttatc agtagctgga gtttctgttt ctacagtttt
    28201 tggagcttct ggttgaagct ctgctttagg tgtttcctca gtccgatttt cggatgattg
    28261 aggggaatca gaaaccgtat ggatggtcgg ttggttttct gtagtagtag gagtaactcc
    28321 atcggctgca acagtctgtg cttggaaggc aaatccaatt agaacagaag ctgctcctac
    28381 agcgtattta cgaatagaaa aacgctgttg tttttcatgt ttcattgcaa aacctcctga
    28441 ttgcattgtt atattgatag cgattatata aatcaacgcc cttattttat ttcttatatt
    28501 aacgagagtc aagaggagat gacaaaaaac tataataagt ataaaaaaat ataaaattta
    28561 aacttaagat ttcagattgg tcggaaaaaa tgcatatata tatctaatat aatttttggt
    28621 tctatttcta taaaatattc cacaaattat agaatcttcc aaaaataggt aagcgctacc
    28681 tttttggtgt ggtataataa gcatagaaaa agcccgagcg attaggctca ggctttcttc
    28741 ttagtgatca cggtcacatg agatgaattt aatcttgtag taatcagatc gtttgtaagt
    28801 ttcactgtat tctaaaactt ggccagttga ttcgagtttg gtgattttag tttgtaggac
    28861 agtagggaat tgttcatcga ctccgaggac tgaagccgca tgttctggag ttggaaagac
    28921 tatttcgttg atttcttcaa agtgttcatc attcatgtga atgtggtagt ctaacttgaa
    28981 acgattatag atagaactat agtattcaag gtttggataa tttgcgttga tatattgttc
    29041 tgggatgtag gatgtatggt agatataaac gacaccgttt gattcgcgga tacgttcaat
    29101 cttgtagtag aattgatcgc cacgtagacc caatttttcc aagtaaacaa gcttgtttcc
    29161 gcgttcaatt gaaagaacag ttaccttatc atctttagca ttgaagagtt caatatctga
    29221 aaactctaca agcttgtgtt tgcgtgcacg tgaaacgaag gttccttttc cttgttggcg
    29281 gacaatatag ccatctttgg caaggtcgtt taaggcgcga acaactgtga tagagctgac
    29341 atcgtacatt gaaatgagtt ctgcttcagt gtaaaattta tctccactgc taaactgccc
    29401 agagatgatt ttatttttta attcgtcttt tatgtattga tatttgggaa ttgccatatt
    29461 tttacctcta ttttccttct ccatagaaaa ttttaccata aaagcgaaaa aaatagtagt
    29521 cttttgaata aaaaattaga ataatagttt gaaaaattaa gcgatatagg gattgctcat
    29581 atttta
//
LOCUS       DAVKAO010000020        28029 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKAO010000020 DAVKAO010000000
VERSION     DAVKAO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28029
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.020"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..407
                     /locus_tag="VWX16_000902"
     CDS             <1..407
                     /locus_tag="VWX16_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9158281.1"
                     /translation="EVDRTPAENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQ
                     LGELYQWFTDTYAHLSLQELKAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKT
                     ATWEVYKFIHVNTVAPSGTFRTKIRKWKKIVL"
     gene            516..1163
                     /locus_tag="VWX16_000903"
     CDS             516..1163
                     /locus_tag="VWX16_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9158282.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(1152..1956)
                     /locus_tag="VWX16_000904"
     CDS             complement(join(1152..1586,1588..1956))
                     /locus_tag="VWX16_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9158283.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANHSGKKKFHAMKAQAIVTSQGRIVSLDIAVNY
                     SHDMKLFKMSCRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            1951..2205
                     /locus_tag="VWX16_000905"
     CDS             1951..2205
                     /locus_tag="VWX16_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9158284.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            2450..3784
                     /gene="trmFO"
                     /locus_tag="VWX16_000906"
     CDS             2450..3784
                     /gene="trmFO"
                     /locus_tag="VWX16_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9158285.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            3868..4605
                     /locus_tag="VWX16_000907"
     CDS             3868..4605
                     /locus_tag="VWX16_000907"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9158286.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            4614..5171
                     /gene="frr"
                     /locus_tag="VWX16_000908"
     CDS             4614..5171
                     /gene="frr"
                     /locus_tag="VWX16_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9158287.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            5231..6085
                     /gene="cvfB"
                     /locus_tag="VWX16_000909"
     CDS             5231..6085
                     /gene="cvfB"
                     /locus_tag="VWX16_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9158288.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPVLKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            6094..6309
                     /locus_tag="VWX16_000910"
     CDS             6094..6309
                     /locus_tag="VWX16_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9158289.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            6395..7363
                     /locus_tag="VWX16_000911"
     CDS             6395..7363
                     /locus_tag="VWX16_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9158290.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(7341..7520)
                     /locus_tag="VWX16_000912"
     CDS             complement(7341..7520)
                     /locus_tag="VWX16_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158291.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            7556..8056
                     /locus_tag="VWX16_000913"
     CDS             7556..8056
                     /locus_tag="VWX16_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9158292.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGFDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            8059..8318
                     /locus_tag="VWX16_000914"
                     /pseudo
     CDS             8059..8318
                     /locus_tag="VWX16_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(8685..9011)
                     /locus_tag="VWX16_000915"
     CDS             complement(8685..9011)
                     /locus_tag="VWX16_000915"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158293.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(8957..9370)
                     /locus_tag="VWX16_000916"
     CDS             complement(8957..9370)
                     /locus_tag="VWX16_000916"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9158294.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(9371..9796)
                     /locus_tag="VWX16_000917"
     CDS             complement(9371..9796)
                     /locus_tag="VWX16_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9158295.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            9973..10542
                     /locus_tag="VWX16_000918"
     CDS             9973..10542
                     /locus_tag="VWX16_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9158296.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            10610..11260
                     /locus_tag="VWX16_000919"
     CDS             10610..11260
                     /locus_tag="VWX16_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU9158297.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTNGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            11244..13484
                     /locus_tag="VWX16_000920"
     CDS             11244..13484
                     /locus_tag="VWX16_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9158298.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSTSYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFFTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGSKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            13663..13851
                     /locus_tag="VWX16_000921"
     CDS             13663..13851
                     /locus_tag="VWX16_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158299.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            13883..14470
                     /locus_tag="VWX16_000922"
     CDS             13883..14470
                     /locus_tag="VWX16_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158300.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            14474..15649
                     /locus_tag="VWX16_000923"
     CDS             14474..15649
                     /locus_tag="VWX16_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158301.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSVS
                     LERELETRLVDRERVINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAMSS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPS
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQKIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    15823..15954
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            15970..16500
                     /gene="infC"
                     /locus_tag="VWX16_000924"
     CDS             15970..16500
                     /gene="infC"
                     /locus_tag="VWX16_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9158302.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            16533..16733
                     /gene="rpmI"
                     /locus_tag="VWX16_000925"
     CDS             16533..16733
                     /gene="rpmI"
                     /locus_tag="VWX16_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9158303.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            16785..17144
                     /gene="rplT"
                     /locus_tag="VWX16_000926"
     CDS             16785..17144
                     /gene="rplT"
                     /locus_tag="VWX16_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9158304.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            17202..17582
                     /locus_tag="VWX16_000927"
     CDS             17202..17582
                     /locus_tag="VWX16_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9158305.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSTKGYEVTEPNGL
                     PGTAPNYYFVKDPDGYKVEVIREK"
     gene            17832..18632
                     /locus_tag="VWX16_000928"
     CDS             17832..18632
                     /locus_tag="VWX16_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9158306.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            18643..19581
                     /locus_tag="VWX16_000929"
     CDS             18643..19581
                     /locus_tag="VWX16_000929"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9158307.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNIKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            19623..21656
                     /locus_tag="VWX16_000930"
     CDS             19623..21656
                     /locus_tag="VWX16_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9158308.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLESD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(21717..23372)
                     /gene="pavA"
                     /locus_tag="VWX16_000931"
     CDS             complement(21717..23372)
                     /gene="pavA"
                     /locus_tag="VWX16_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9158309.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            23477..23974
                     /gene="ybeY"
                     /locus_tag="VWX16_000932"
     CDS             23477..23974
                     /gene="ybeY"
                     /locus_tag="VWX16_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9158310.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            23955..24350
                     /locus_tag="VWX16_000933"
     CDS             23955..24350
                     /locus_tag="VWX16_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9158311.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            24367..25266
                     /gene="era"
                     /locus_tag="VWX16_000934"
     CDS             24367..25266
                     /gene="era"
                     /locus_tag="VWX16_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9158312.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            25315..26139
                     /gene="mutM"
                     /locus_tag="VWX16_000935"
     CDS             25315..26139
                     /gene="mutM"
                     /locus_tag="VWX16_000935"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9158313.1"
                     /translation="MPELPEVETVCRSLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFCLTDKVLISHLRMEGKYFYYPDQVPERKHAHVFFRFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            26139..26744
                     /locus_tag="VWX16_000936"
     CDS             26139..26744
                     /locus_tag="VWX16_000936"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9158314.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            26731..27846
                     /locus_tag="VWX16_000937"
     CDS             26731..27846
                     /locus_tag="VWX16_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEU9158315.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIKE
                     DFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVRDLGQTE
                     NLLFVSGLIVSSMGFSSMMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANASSPL
                     QLGLYRFLFGLGTGALIPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGSAV
                     AGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI"
     gene            27846..27995
                     /gene="rpmG"
                     /locus_tag="VWX16_000938"
     CDS             27846..27995
                     /gene="rpmG"
                     /locus_tag="VWX16_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9158316.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
BASE COUNT         8321 a         5169 c         6333 g         8206 t
ORIGIN      
        1 atgaagttga cagaactcca gcagaaaacc ttgcttatca ggttggttgg accaacttgg
       61 ttcttaaatg ggaagaagat gaaagaaagg ggcttcaagt aaaaacacca tcggataaat
      121 ttaaatggaa tcaacttggt gaattatatc agtggttcac agatacctac gctcatttat
      181 ctctgcaaga gttgaaagca aaattaaatg aaaatattaa ttctatctct gcaatgattg
      241 attcgttgag tgaggaagaa ttatttgaac cgcatatgag aaagtgggct gatgaagcga
      301 ctaaaacagc gacttgggaa gtgtataagt ttattcatgt aaatacggtt gcaccttctg
      361 gaactttcag aactaaaatc agaaaatgga agaagatagt attataaatt atatttttaa
      421 ctttaaaaaa tttcataaaa atggttacca aaggcgatag aagaaaaact atcgtctttt
      481 tctttgcaaa tttttaagaa gggaggtgat cttgcatgga ctttgaatat ttttataaca
      541 gagaagcgga aagatttaac ttcttaaaag taccggagat attagttgat agagaagaat
      601 ttcggggctt atcagcagaa gcaattatcc tttattccat acttcttaaa cagacaggaa
      661 tgtcatttaa gaataactgg atagacaagg aaggcagagt atttatctat tttactgtcg
      721 aagaaattat gaaaagaaga aatatctcaa agccaactgc cataaaaaca ttagatgagc
      781 ttgatgtaaa aaaaggaata ggactgatcg aaagagtaag gcttggactt ggtaagccga
      841 acatcattta tgttaaagac tttatgagta tatttcaggt aaaagaaaat gacttacaga
      901 agtcaaaaaa cttaacttca gaagtaaaag attttaacct cagaagtaaa gaaaatgaac
      961 ttcaagaggt taagaacctt gactctaact atatagagaa taataagagt aagtatagta
     1021 agagagaata tagttttggt gaaaacggac ttggaacatt tcaaaatgtg tttttagctg
     1081 ctgaagatat atcggattta caaatcataa tgaactcaca gcttgagaat tacattagac
     1141 ttcctgcaaa actagaatcc tagttcatga ttgataatac cagcaatcaa attcattcgt
     1201 aatccgaagc gtttacgatg atttcgatag gttgttgaaa acattttaaa cgtttttact
     1261 ttggcaaaga tgttctcaac cttgcttctc tccttagata gcgcatggtt acaggcttta
     1321 tcttcagctg ttagcggctt gagtttgctg gatttacgtg gagtttgtgc ttgaggatat
     1381 atcttcatga gcccttgata accactgtcg gccaagattt taccagcttg tccgatattt
     1441 ctgcaactca ttttgaacaa cttcatatca tgactatagt tcacagcgat atccaaagaa
     1501 acaattctcc cttgacttgt gacaatcgct tgagccttca tagcgtgaaa tttcttttta
     1561 ccagaatgat tcgctaattc tttttttagg gcgattgatt tgtacttccg tcgcatcaat
     1621 cattaccgtg tcctcagaac tgagaggagt tcttgaaatc gtaacaccac tttgaacaag
     1681 agttacttca acccattggc tccgacggat taagttgctt tcgtgaatac caaaatcagc
     1741 cgcaatttgt tcataagttc gatattctcg cacatattga agagtggcca taagaaggtc
     1801 ttctaggctt aatttaggtt ttcgtccacc ttttgcgtgt ttaagttgat aagctgtttt
     1861 taatacagct aatatctctt caaaagtcgt gcgctgaaca ccaacaagac gcttaaatcg
     1921 tgcatcagtt agttgtttac ttgcttcatc attcatagaa ctactatacc atattttgtt
     1981 tcgcaggaag tctattggaa agtaagaaat attgaagctg aggctattag aagaaattgt
     2041 gagcgtggtg ctattttttc aggtaaaata aaatatcacg aagattcaca gtttaaagga
     2101 gatcactatg ttgaatgtta tgctgtttta gataatacgg ttatagcaag agatagaata
     2161 acagtcccta tcgatccgtt atgtggaaaa gattttatag agtagcatat aattgattct
     2221 taactggaat actcactatc tctttacatc aagaaaatga ctaaacaggg aagtttgcct
     2281 tcttcccttt ttttgttata ctagtagaag aaaaaataga aagatttgtg ggagtgaaaa
     2341 agccctgtgg actttttcag cctgagccaa gaaactcgaa agcttgtaag tctgattggc
     2401 tttttcaatg tgaatcttaa cttcacactc ccaaagaggt attagtgtcg tgtctcaatc
     2461 ttatatcaat gttatcggtg ctggtttggc aggttctgaa gcagcttacc aaatcgcaga
     2521 gcgtggtatt ccagttaaac tatatgaaat gcgtggtgtc aagtctacac cccagcataa
     2581 aacagacaat tttgctgagt tggtttgttc caattctttg cgtggggatg ctttgacaaa
     2641 tgcagttggt cttctcaagg aagaaatgcg tcgcttgggt tctgttatct tggaatctgc
     2701 tgaggctaca cgtgttcctg ccggtggagc ccttgcggtg gatcgtgatg gtttctctca
     2761 aatggtgacc gaaaaagtgg tcaaccaccc cttgattgaa gtggtgcgtg atgaaattac
     2821 agaattgcca acagatgtta ttacagttgt ggcgactggt cccttgacta gcgatgccct
     2881 ggctgaaaag attcatgccc ttaataatgg cgatggcttc tatttctacg atgcagcagc
     2941 gcctattatc gatgtcaaca ctatcgatat gagtaaggtc tatctcaaat ctcgttatga
     3001 taagggagaa gcagcctatc tcaatgctcc aatgaccaag caagagttta tggatttcca
     3061 tgaagccttg gtcaatgcgg aagaagcacc gctcaattct tttgaaaaag aaaagtactt
     3121 tgaaggatgt atgccaatcg aagttatggc caaacgtggc attaaaacta tgctttatgg
     3181 ccctatgaag ccagtcggtc ttgagtatcc ggacgactac acaggacctc gtgatggaga
     3241 atttaaaaca ccttatgcgg ttgtgcaact tcgtcaggat aatgcagctg gtagcctata
     3301 caatattgtt ggtttccaga cccacctcaa atggggagaa caaaagcgtg tcttccaaat
     3361 gattccgggt cttgaaaatg cggagtttgt ccgttatggt gtcatgcatc gcaattctta
     3421 catggattca ccaaatcttc ttgagcagac ttaccgttcc aagaaacaac caaatctctt
     3481 ctttgctggt cagatgacgg gggtggaagg ctatgttgag tcggcggctt cgggcttagt
     3541 tgcgggaatt aacgcagctc gtctcttcaa ggaagaaagc gaggttattt tccccgagac
     3601 gacagcgatt ggaagcttag ctcattacat tacccatgcc gacagcaaac atttccaacc
     3661 aatgaatgtc aattttggga tcatcaagga gttggaaggc gagcgtatcc gtgataagaa
     3721 ggctcgttat gaaaaaattg cagagcgtgc ccttgccgac ttagaggaat ttttgactgt
     3781 ctaatttttt tgaaagaatt gttcatgata ctataaaaat cttagaaatt gtgataaaat
     3841 aggtaggatg aaagaaggag agtgaaaatg gcgaatccca agtataaacg tattttaatc
     3901 aagttatcag gtgaagccct tgccggtgaa cgtggcgtag ggattgatat ccaaacagtt
     3961 caaacaatcg caaaagagat tcaagaagtt catagcttag gtatcgaaat tgcccttgtt
     4021 attggtggag gaaatctctg gcgtggagaa cctgcagcag aagcaggtat ggaccgtgtt
     4081 caggcagatt acactggaat gcttgggact gttatgaatg ctcttgtgat ggcagattca
     4141 ttgcaacaag ttggggttga tacgcgtgta caaacagcta ttgctatgca acaagtggca
     4201 gagccttatg tccgtggacg tgcccttcgt caccttgaaa aaggccgtat cgttatcttt
     4261 ggtgctggaa ttggttcacc atacttctcg acagatacaa cagcggccct tcgtgcagct
     4321 gaaatcgaag cagatgccat cctcatggct aaaaatggcg tcgatggtgt gtacaatgcc
     4381 gatcctaaga aggacaagac agccgttaag tttgaagaat tgacccaccg tgatgttatc
     4441 aacaaaggtc ttcgtatcat ggactcaaca gcctcaaccc tctcaatgga caacgacatt
     4501 gacttggttg tcttcaacat gaaccaatca ggcaacatca aacgtgtcgt atttggtgaa
     4561 aatatcggaa caacagtttc aaataatatc gaagaaaagg aataagaaag aatatggcta
     4621 acgtaattat tgaaaaagct aaagagagaa tgacccagtc tcaccaatca cttgctcgtg
     4681 aatttggtgg tatccgtgct ggtcgtgcca atgcaagctt gcttgaccgt gtacatgtag
     4741 aatactatgg agtcgaaact cctcttaacc aaatcgcttc aattacgatt ccagaagcgc
     4801 gtgttttgtt ggtaacacca tttgacaagt cttcattgaa agacatcgaa cgtgccttga
     4861 acgcttctga tcttggtatc acaccggcta atgacggttc tgtgattcgc ttggttatcc
     4921 cagctcttac agaagaaact cgtcgtgacc ttgctaaaga agtgaagaag gtcggcgaaa
     4981 atgctaaagt ggctgtccgc aatatccgtc gcgatgctat ggacgaagct aagaaacaag
     5041 aaaaagcaaa agaaatcact gaagacgaat tgaagactct tgaaaaagat attcaaaaag
     5101 taacagacga tgctgttaaa cacatcgacg acatgactgc taacaaagag aaagaacttt
     5161 tggaagtcta aaaataaaca gaaaaactca gttggcattg ctggctgagt tttattcgaa
     5221 agaaggaaat atgaatacaa atcttgcaag ttttatcgtt ggactgatca tcgatgaaaa
     5281 tgaccgtttt tactttgtgc aaaaggatgg tcaaacctat gctcttgcta aggaagaggg
     5341 ccaacataca gtaggggata cggtcaaagg ttttgcatac acggatatga agcaaaaact
     5401 ccgcctgaca accttagaag tgactgccac tcaggaccaa tttggttggg gacgtgtcac
     5461 agaggttcgt aaggacttgg gtgtctttgt ggatacaggc cttcctgaca aggaaatcgt
     5521 tgtgtcactc gatattctcc ctgtgctcaa ggaactctgg cctaagaagg gcgaccaact
     5581 ctacatccgt cttgaagtgg ataagaaaga ccgtatctgg ggcctcttgg cttatcaaga
     5641 agacttccaa cgtctcgctc gtcctgccta caacaacatg cagaaccaaa actggccagc
     5701 cattgtttac cgtctcaagc tgtcaggaac ttttgtttac ctaccagaaa ataatatgct
     5761 tggttttatt catcctagcg agcgttacgc agagccacgt ttggggcaag tattagatgc
     5821 gcgcgttatt ggtttccgtg aagtggaccg cactttgaac ctctccctca aaccacgctc
     5881 ctttgaaatg ttggaaaacg atgctcagat gattttgact tatttggaaa gcaatggcgg
     5941 tttcatgacc ttaaatgaca agtcatctcc agacgacatc aaggcaacct ttggcatttc
     6001 taaaggtcag ttcaagaaag ctttaggtgg tcttatgaag gctggtaaaa tcaagcagga
     6061 ccagtttggt acagagttga tttagggagg cttatgagaa aatcatttta cacttggctc
     6121 atgaccgagc gcaatcctaa aagtaacagt cccaaagcaa ttttggcaga cctcgctttt
     6181 gaagaggcag cttttccaaa acatacagat gattttgatg aggtcagtcg ctttttggag
     6241 gagcatgcca gtttctcttt taacctagga gattttgaca gcatttggca ggaatatcta
     6301 gaacactagc atttattcat tgggtttggg ctagtaattt ctccatccct ctgctataat
     6361 aaaaagaaat aaaaggatta gagaggttct ttatttgaag gaacattcaa tagacattca
     6421 actgagtcat ccagatgacc tgtttcatct ttttggttcc aatgagcgcc atcttcgttt
     6481 gatggaagaa gagctggatg tggtgattca tgctcgtacg gagattgtcc aggttttggg
     6541 agaagagtct gcttgtgagg aagcccgtca ggttattcaa gctctcatgg tcttggtgaa
     6601 ccgtgggatg accgttggta cgccagatgt ggtaactgca attagcatgg tcaaaaacga
     6661 tgaaattgat aagtttgtcg ccctttacga agaagaaatt atcaaagata atactgggaa
     6721 accgatccgc gtcaaaacct taggtcaaaa actttatgtg gacagtgtca aacagcatga
     6781 tgtgaccttt ggaattgggc ctgcagggac ggggaagacc tttcttgcag ttactttggc
     6841 agtgactgcc cttaaacgtg ggcaagtcaa gcggattatc ttgacacgtc cagcggtgga
     6901 agcaggagag agtctaggtt ttcttccggg tgatcttaag gagaaggtag atccttacct
     6961 tagacctgtt tatgatgcct tgtatcagat tctggggaaa gaccaaacaa ctcgactcat
     7021 ggagcgtgaa attatcgaga ttgcgcccct tgcctatatg cgtggacgga ccttggatga
     7081 tgcctttgtc attctcgatg aggcgcaaaa tacgaccatc atgcagatga agatgttctt
     7141 gactcgttta ggttttcatt ctaagatgat tgtcaatgga gatattagtc agattgacct
     7201 gccacgtaat gtcaagtccg gtttgattga tgctcaagag aaactcaaga acatccatca
     7261 gattgacttt gttcattttt cagccaagga tgtggttcgc catcctgttg tcgctcagat
     7321 tatccgagcc tatgaatatt ctactgaagt tgcacacgac tgattttgag gaagttcgcc
     7381 tgcaaaagaa tatacttttg agagaggaaa atccagttgt ataggctaaa ggttttatcc
     7441 aaaggtttga gacaacgatt aggcacgatg gaaagaactt ttatgtggct gatgacgatc
     7501 agtgcatctt cctgtgtcat aatcacaggg cacaagaaag taggaatttg aaaagatgat
     7561 tgaccaacta tctaagtatt acagttgtag gatactaact gaaaaggata ttccaagtat
     7621 tttatcttta tatgaaagta atcctctgta ttttcagcat tgtccaccag agccaaattt
     7681 tgcaactgta aaagaggaca tgctttgtct acctgaaggt aaagctaagg ctgataagtt
     7741 ttttgttgga ttttggaatg gatttgacct tgtggctgtt atggattttg tctatgcata
     7801 tcctgatgag gagactgttt ttattggttt gtttatggtt gatcaagcct atcagaggaa
     7861 agggattggt agtcatattg tgacagaagc actagcttat tttgctaaga actttcgaaa
     7921 ggcacgtttg gcttatgtta agggaaatcc gcaatctcag catttttggg aaaagcaggg
     7981 ctttaaatca attggatgcg aggttaagca agaactctat acggttgtta tcgttgaaca
     8041 gagcctagaa gattagaaat ggcatcaagt aagaactatt tggaatttgt tttggaacaa
     8101 ttatcaggat tagatgatgt gacttaccgt tccatgatgg gggagtatat tctttacttc
     8161 cgcggcaaga ttattggcgg catttatgac gatcgctttt agttaaaccc gtgcaagcag
     8221 tcttagataa gattgaccaa tcttcttttg agtttccata caaaggtgcc aaagaaatga
     8281 tttgagtgga agaacttgat aataagatgt ttctataaga cctgatttta gctatgtata
     8341 accaactgcc aacgcccaaa cctaaaaaga aaaagcaagg gtgaacgaag taaaaaagaa
     8401 gtctgctaag gccctgtctt tgcacgggta aaattttata tataaaaaga agctgggact
     8461 aaagagctca gcttcctttg gtttatataa ttgtcattac aagacgaagt ggttgggcga
     8521 aactctgttg actttattca atttagagtt tcttatgcac aattgagtct ggaacgaaag
     8581 tctccagttg caaagtatac agtacaataa accaacgatg taatagctga tgacacaaag
     8641 cacagtgggt aggacttgcg aagtcaccct tttcttttca aaatttatac taaatcattg
     8701 atatcagtgt agtcacgatt aagtccttga gcaactggta ggctagtcaa gtaaccttga
     8761 taagtggtca caccttgacg caagccttca tcttcagaga ttgcttgtgc gaatcctttg
     8821 ccagccaaag cttcgatata aggaagagtg acattggtta gggcgatggt tgaagtgcgg
     8881 gcaaccgcac cagggatatt ggcaacggca tagtggagaa caccgtgttt ttcatagacg
     8941 ggttcatcgt gcgttgtcac acggtcagct gtttcgataa cgccaccttg gtcaacagca
     9001 acgtcaacga tacagagcct ggacgcattt gtttgaccat ctcatctgtc accaattccg
     9061 gtgcttttgc accagggatg agaatggctc caatcaccac atcagcatct ctcatacttg
     9121 cttcaatgtt gaatgaatta gatataagag tttgaatttg acttccaaag acttcttcta
     9181 gaactgagag acgcttggca ctaatatcta aaatagtcac ttgagcacca agaccaaggg
     9241 cgatgcgggc agcatgtgta ccgacgacac catcaccgat gatagttact tttccttttg
     9301 gaacacctgg tacaccacca ggtagaacac cagagccacc agcttgctta gtaaggaagt
     9361 gagctccgat ttaaacagcc atacgacctg caacctcact cataggaacg aggagcggta
     9421 gttgtccttg attgtcacga acagtttcat aggcaattcc tgttgttttt gctgctaaca
     9481 tagcatctgc taattctgga gcagcggcca tgtgcaagta ggtgaagaga agaagatcgt
     9541 cgcgcaagta accgtattca gaacttaaag gttcttttac tttcacaacc aactctgctg
     9601 cccaagcttc accagcagta gcgacaatct cagctccttg cttttgatag tcagcatcag
     9661 taaagccaga accgagacca gcatttgttt cgataaggac acgatgacca cgactaacta
     9721 agctatgaac acctgcaggt gtgagggcga cacggttttc gttattttta atttcttttg
     9781 ggattccgat taacattgag ataacctacc tttcaattga cggtcttgtt ttggttgtca
     9841 cattccagtt cataaatcaa aaatgtgacg gtttcattgt atatgaaacc gcttcaaaaa
     9901 tcaagaaaaa cttgtcatcc aaatttttta tgctagacta gtgaaaatca agctttgatt
     9961 ggttggaaaa gtatggaatc aatttttgta aaacttgccc agtatccgtc tatagaaacg
    10021 gagcgtttat tgcttagacc tgtaactttg gatgatgcgg aagcaatgtt tgactatgcc
    10081 tcggacaagg gtaatacacg ttacactttt ccaaccaatc aaagcttgga agaaaccaag
    10141 aataacattg ctcagttcta tttggctaat cccttgggac gttggggaat agaactaaaa
    10201 agcaatggtc agtttattgg aaccattgac ttgcacaaga ttgattctgt tcttaagaag
    10261 gcagctattg gctacattat caataaaaag tattggaatc aaggattaac gacagaagcc
    10321 aatcgtgctg tgattgagct agcttttgag aagataggga tgaataagtt gactgccctt
    10381 cacgataagg acaatcctgc gtcaggaaag gtcatggaga aatcaggcat gcgtttttcc
    10441 catgcagaac catatgcttg tatggaccag catgaaaaag atcgaatcgt tacaagagtt
    10501 cattatgtct tgaccaagga agactatttt gcaaataaat aagcagttga aaagaaattt
    10561 ttcgactgtt ttttcttcct cttacgaata atctaagaga ggagaaaata tggaagcaat
    10621 tatcgagaaa atcaaagagt ataaaatcat cgtcatctgt actggtctgg gcttgcttgt
    10681 aggcggattt ttcctgctaa aaccagctcc acaaacacct gtcaaagaga cgaatttgca
    10741 ggctgaagtc gcagctgttt ccaaggattt ggtatccgaa aaggaagtga acaaggaaga
    10801 aaaggaagaa ccccttgaac aagatctaat cacagtagat gtcaaaggtg ctgtcaaatc
    10861 gccagggatt tatgacttgc ctgtaggtag tcgagtcaat gatgctgttc agaaggctgg
    10921 tggcttgaca gagcaagcag acagcaagtc gctcaatcta gctcagaaag ttagtgatga
    10981 ggctctggtt tacgttccta ctaatggaga agaagcagtt agccaacaga ctggtttggg
    11041 gacagcttct tcaataagca aggaaaagaa ggtcaatctc aacaaggcta gtctggaaga
    11101 actcaagcag gtcaagggac tgggaggaaa acgagctcag gacattatcg accatcgtga
    11161 ggcaaatggc aagttcaaat cagtagacga gctcaagaag gtctctggca ttggtgccaa
    11221 gactatagaa aagttaaaag actatgttac agtggattaa gaatttctct attcccctaa
    11281 tttacctgag ttttctgtta ctttggcttt actacgccat tttttcaaca tcctatcttg
    11341 ctttgttggg atttgttttt ctgctagtct gtctcttttt ccaatttcct tggaaatctg
    11401 ctagcaaagt tctagtgatt tgtggaatct ttggattttg gtttgttttt caaaattggc
    11461 aacagagtca agcgagtcaa aatctggcgg attctgttga aagggtacgg attttgcctg
    11521 atactattaa ggttaatggt gatagtctat cctttcgtgg caagtctaac ggtcgtgctt
    11581 tccaagtcta ttataaactc cagtccgagg aggagaaaga agcctttcaa gctttaaccg
    11641 acctgcatga gataggacta gaagggaagc tttcggagcc agaagggcag agaaattttg
    11701 gtggctttaa ttaccaagcc tatctgaaga ctcagggaat ttaccagact ctcaatatca
    11761 aaacaatcca gtcacttcaa aagattggca gttgggatat aggagaaaac ttgtccagtt
    11821 tacgtcgaaa ggctgtggtt tggattaaga cgcactttcc agaccctatg cgcaattaca
    11881 tgacaggact cttgctggga catctggaca ccgactttga ggagatgaat gagctttatt
    11941 ccagtctagg aattatccac ctctttgccc tatctggcat gcaggtaggt tttttcatga
    12001 atggatttaa gaaacttctc ttgcgattgg gcttgaccca agaaaagttg aaatggctga
    12061 cttatccctt ttcccttatc tatgcgggac taactggatt ttcagcatcg gttattcgca
    12121 gtctcttgca aaagctactg gctcaacatg gggttaaggg cttggataat tttgccttga
    12181 cggtgcttgt cctctttatt gtcatgccaa actttttctt tacagcagga ggagtcttgt
    12241 cctgcgctta tgcttttatc ctgaccatga ccagcaaaga aggggagggg ctcaaggctg
    12301 ttgctagtga aagtctagtc atctccttgg gcatattgcc cattctatcc ttctattttg
    12361 cggaatttca accttggtct atccttttga cctttgtctt ttcctttcta tttgacttga
    12421 ccttcttacc gctcttgtct attttatttg tcctttcctt tctctatcca gtcattcagc
    12481 tgaactttat ctttgaatgg ttagagggca ttattcgctt ggtctcgcag gtggcaagga
    12541 gaccgcttgt ctttggtcaa cccaacgcat ggcttttaat cttattgtta atttccttgg
    12601 ctttggtcta tgatttgagg aaaaacatta aaggattaac agtattgagt ttattgatta
    12661 caggtctctt tttccttacc aagtatccac tggaaaatga aatcaccatg ctggatgtgg
    12721 ggcaaggaga aagtattttc ctacgggatg taactgggaa aaccattctc atagatgtag
    12781 gtggtaaggc agaatcttat aagaaaatcg aaaaatggca agaaaagatg acgaccagca
    12841 atgcacagag aagcttgata ccctatctta aaagtcgagg agtagctaag attgaccagc
    12901 tgattttgac aaatacggac aaggaacatg tcggagattt gttagaggtg accaaggctt
    12961 tccatgtagg ggagatttta gtatcaaaag gcagtttgaa gcagaagcaa tttgtggtag
    13021 aactacaggc gactcaaaca aaggtgcgta gtatgacagt aggggagaac ttgcccattt
    13081 ttggaagtca gttagaagtt ctatctccaa ggaaaatggg agatggagga cacgatgata
    13141 ccctagttct gtatgggaaa ttcttggata agcaatttct cttcacggga aatttggagg
    13201 agaaaggaga gaaggacttg ctgaagcact atccagactt gaaagtaaat gttttgaaag
    13261 ctagccaaca tggctctaaa aaatcatcaa gtccagcctt tctagaaaaa ctcaaaccag
    13321 agcttactct tatctcagtt ggaaagagca atcgaatgaa actcccccat caggaaacat
    13381 tgacacgact ggaaggtatc aatagcaaag tttatcgaac tgaccagcaa ggagctatac
    13441 gttttaaggg gttggatagt tggaaaatcg aaagtgttcg ataggaagga taaatgttgt
    13501 agattagtga aataaactaa aaatttgttg cataataatg ataaaaatgg tataatgaaa
    13561 acgtattcaa tattgaggat ataaaatcat taaaaatcag caaaagttgt tttattagtt
    13621 agtttataat ctattggtat tcttcagtcc agtgtatctg ctgtgacagc cactaaaagt
    13681 tacaagtatg attggaatac ggttttggaa tatagtacca actatcacga ccatcagtat
    13741 gcttggattc cggaatggtc tcgttattac agctattctg agtataaagt tggcggaggc
    13801 tggaactacg ctcgttatga ggtcataaac tactatactg gaggttatta atccttaaag
    13861 agtgagaaaa ggagggctgg atatgttaaa tcttactcat gttaccttaa aaacgcgaca
    13921 agtcatcttg caagatgcgg attttacctt taaaaagggt aggatttatg gccttcttgc
    13981 tatcaatggc tcgggaaaga cgacactatt ccgagctatg agcaagttgc ttccccttag
    14041 tagtggacac atcgcagttc ctccttcttt gttttattat gagagcgttg aatggctgga
    14101 tggaaactta agtgggatgg actaccttcg tctcataaaa aacatctgga agtcagacct
    14161 aaacttgaga gatgaaatcg cctactggga aatggctgac tatatcagtc ttcccatccg
    14221 caagtattcc ttagggatga agcaacgctt ggtgattgct atgtattttc tcagtcaggc
    14281 caaatgctgg ctcatggatg agattacaaa tggcttagac gagtattata gacagaagtt
    14341 ttttgatagg ctagcacaaa tcgatagaca agaacagctg gttcttttaa gttcccacta
    14401 taaggaagag ttggttgata tctgcgatag agtagtaacc attcatcagg ggcagataga
    14461 agaggtttag tttatgaaag atgttagtct atttttattg aaaaaagttt tcaaaagtcg
    14521 tttaaactgg attatcttac ttttatttgc atctgtactc ggtgttacct tttatttaaa
    14581 tagtcagact gcaaactcag tcagcttgga gagagagttg gaaactcgtc ttgtagaccg
    14641 tgagagagtc atcaatgaaa atgaagagaa actctcccaa atgtctgata ccagctcgga
    14701 ggaataccag tttgctaaaa ataatttaga cgtgcaaaaa aatcttttga cgcgaaagac
    14761 agaaattctg actttattaa aagaagggcg ctggaaagag gcctactatc tgcagtggca
    14821 agatgaagag aagaattatg aatttgtatc aaatgacccg actgctagct ctggcttaaa
    14881 aatgggggtt gaccgcgaac ggaagattta ccaagccctg tatcccttga acataaaagc
    14941 acatactttg gagtttccga cccacgggat tgatcagatt gtctggattt tagaggttat
    15001 catcccaagc ttgtttgtgg ttgcgattat ttttatgcta acacaactat ttgcagaaag
    15061 atatcaaaat catctggaca cagctcactt atatcctgtt tcaaaagtga catttgcaat
    15121 gtcttctctt ggagttggag tgggctatgt aactgtgctg tttatcggaa tctgtggctt
    15181 ttcttttcta gtgggaagtc tgataagtgg ttttggacag ttagattatc cctacccgat
    15241 ttatagctta gtgaatcaag aggtaactat tggaaagatg caggatgtat tatttcctag
    15301 cttgctctta gctttcttag cctttatcgt cattgtggaa gttgtgtatt tgattgctta
    15361 ctttttcaag caaaaaatgc ctgtcctctt tctttcactc attgggattg ttggcttatt
    15421 gtttggcatc caaaccattc agcctcttca aaagattgca catctgattc cctttactta
    15481 cttgcgttca gtggagattt tatctggaag attacctaag cagattgata atgtcgatct
    15541 aaattggagc atgggaatgg tcttacttcc ttgcctgatt atctttttgc tattgggaat
    15601 tctatttatt gaaagatggg gaagttcaca gaaaaaaaga attttttaat agattctagc
    15661 tttcctatag gtagggaaaa taagtaaaaa ctaacataga gagggaatca acttgattct
    15721 ctctttttga ttcgaaaacc aaaccaaaat acaaacacaa acttttcaaa aaataacttt
    15781 ttatcttgac aagagctaga aaacttggta tcatataaaa gttgagaaaa gcagaagtga
    15841 gagcttctcg ccttgtgaca ttaagttgcc tggccctacg gatgaaaagt ttcgaagaaa
    15901 cgctatcata acgtgcgggc ttgtatattt acgagtccgc tattgttttt ctctaataaa
    15961 acaaaagagg tgaaaaccat agcaaagcaa gacttattca tcaatgatga gattcgtgta
    16021 cgtgaagttc gcttgattgg tcttgaagga gaacagctag gcatcaagcc actcagtgaa
    16081 gcgcaagctt tggctgataa cgctaatgtt gacctagtat tgattcaacc ccaagccaaa
    16141 ccgcctgttg caaaaattat ggactacggt aagttcaaat ttgagtacca gaagaagcaa
    16201 aaagaacaac gtaaaaaaca aagcgttgtt actgtgaaag aagttcgtct aagtccaact
    16261 attgacaagg gtgactttga tacaaaactt cgtaatgcac gtaagttcct tgaaaaagga
    16321 aataaagtta aggtatctat tcgctttaag ggtcgtatga ttacccataa agagattggt
    16381 gcaaaagttt tagccgagtt tgctgaagca actcaagata ttgccatcat cgaacaacgt
    16441 gctaaaatgg atggacgtca aatgttcatg caattggcgc cagcgactga caaaaaataa
    16501 ttgtcagaaa gttaaataaa ggagaaaaaa tcatgccaaa acaaaaaaca caccgcgcat
    16561 cagctaaacg tttcaaacgt acaggttctg gtggacttaa acgtttccgt gcttacactt
    16621 ctcaccgttt ccacggaaaa actaagaaac aacgtcgtca tcttcgtaaa gcatctatgg
    16681 tgcattcagg agattacaaa cgtatcaaag caatgcttac tcgcttgaaa taatagcttg
    16741 actgtaagta aacaattcta ggaaatattt ggaggaaata aaatatggca cgtgttaaag
    16801 gtggcgttgt atcacgcaaa cgtcgtaaac gtattcttaa attagcaaaa ggttactatg
    16861 gagctaaaca catcttgttc cgtactgcaa aagaacaagt aatgaactct tactactatg
    16921 cataccgtga ccgtcgtcag aaaaaacgtg acttccgcaa attgtggatt actcgtatca
    16981 atgcggcagc tcgtatgaac ggactttcat actcacaatt gatgcatggt ttgaaattgg
    17041 ctgagatcga agttaaccgt aaaatgcttg ctgacttggc tgttaacgat gcagtagctt
    17101 tcacagctct tgcagatgca gctaaagcaa aacttggtaa ataacaactg atgagactgg
    17161 aacaggattg tccagtcttt ttaaaaataa aggagaaaga tatggcttca aaaatgctac
    17221 acacttgctt acgagtagaa aatcttgaaa aatcaattgc attttatcaa gatgcttttg
    17281 gttttaaaga attgcgtcgc agagattttc cagaccatgc cttcacgatt gtctatctag
    17341 gtcttgaggg tgatgactat gagttggagt tgacttataa ctacgatcac ggtccgtatg
    17401 tggttggtga tgggtttgcc catatcgccc tcagtacacc tgatcttgag gcacttcatc
    17461 aagagcacag tacaaaaggc tatgaagtga ctgagccaaa tggtctacca ggaactgcac
    17521 caaactatta ctttgtcaaa gaccctgatg gatataaggt cgaagtcatt cgtgaaaaat
    17581 aatttcgtgc aagttaagta gaatattcgt tttctacttg acttttttta gctgaaagag
    17641 tatactaata aaaattcaac ctttaagggg agtccagaga gactcacaag gtgtcagata
    17701 aaagaatagt gcaattttct agaggagacc ttttgagtgt gctctcttgt gttgtacgat
    17761 tttaactgag gccttgcact agcaaggtct tttctttatc tggtcccctt aaaatttaag
    17821 gaggaaaagt tatgaatccc acatgtaaga agcgtttggg tgtcattcgg ttggaaacca
    17881 tgaaggtggt tgcacaagag gaaatcgcgc cagcaatctt tgaattagtc ctagaaggag
    17941 aaatggttga agccatgcga gcaggccaat ttcttcatct gcgtgtaccg gacgatgccc
    18001 atctcttacg tcgtcctatt tcaatttcgt ctattgacaa ggccaacaag cagtgtcacc
    18061 tcatttatcg gattgaggga gctgggactg caattttttc aaccttaagt cagggagaca
    18121 ctcttgatgt gatggggcct cagggaaatg gttttgactt gtctgacctt gatgagcaga
    18181 atcaggttct ccttgttggt ggtgggattg gtgttccacc cttgcttgag gtggccaagg
    18241 aattgcatga acgtggagtg aaagtagtga cagtcctcgg ttttgctaat aaggatgctg
    18301 ttattttgaa aacggaattg gctcagtatg gtcaggtctt tgtaacgaca gatgatggtt
    18361 cttatggcat caagggaaat gtttccgttg ttatcaatga tttagatagt cagtttgatg
    18421 ctgtttactc gtgtggggct ccaggaatga tgaagtatat caatcaaacc tttgatgatc
    18481 acccaagagc ctatttatct ctggaatctc gtatggcttg tgggatggga gcttgctatg
    18541 cctgtgttct aaaagtacca gaaagcgaga cggtcagcca acgcgtctgt gaagatggtc
    18601 ctgttttccg cacaggaaca gttgtattat aaggagaaaa ttatgactac aaatcgatta
    18661 caagtgtctc tacctggttt ggatttgaaa aatccgatta ttccagcatc aggctgtttt
    18721 ggctttggac aagagtatgc caagtactat gatttagacc ttttaggttc tattatgatc
    18781 aaggcgacaa cccttgaacc acgttttggg aatccaactc caagagtggc agagacgcct
    18841 gctggtatgc tcaatgcaat tggcttgcaa aatcctggtt tagaggttgt tttggctgaa
    18901 aagctacctt ggctggaaag agaatatcca aatcttccta ttattgccaa tgtagctggt
    18961 ttttcaaaac aagagtatgc agctgtttct catgggattt ccaaggcaac taatataaaa
    19021 gctatcgagc tcaatatttc ttgtcccaat gttgaccact gtaatcatgg acttttgatt
    19081 ggtcaagatc cagatttggc ttatgatgtg gtgaaagcag ctgtggaagc ctcagaagtg
    19141 ccagtttatg tcaaattaac cccgagtgtg accgatatcg ttactgtcgc aaaagctgca
    19201 gaagatgcgg gagcaagtgg cttgactatg atcaatactc tggttggaat gcgctttgac
    19261 ctcaaaacca gaaaaccaat cttggccaat ggaacaggtg gaatgtcagg tccagcagtc
    19321 tttccagtag ccctcaaact catccgccaa gtagcccaaa caacagacct gcctatcatt
    19381 ggaatggggg gagtggattc ggctgaagct gccctagaaa tgtatctggc tggggcatct
    19441 gctatcggag ttggaacagc taactttacc aatccttatg cctgccctga catcatcgaa
    19501 aatttaccaa aagtcatgga taaatacggt attagcagtc tggaagaact ccgtcaggaa
    19561 gtaaaagagt ctctgaggta aactgcaatc aatctgttct tgatttttta ttagtttgta
    19621 atatgaattt aggagaattt tggtacaata aaataaataa gaacagagga agaaggttaa
    19681 tgaagaaagt aagatttatt tttttagctc tgctattttt cttagctagt ccagagggtg
    19741 caatggctag tgatggtact tggcaaggaa aacagtatct gaaagaagat ggcagtcaag
    19801 cagcgaatga gtgggttttt gatactcatt atcaatcttg gttctatata aaagcagatg
    19861 ctaactatgc tgaaaatgaa tggctaaagc aaggtgacga ctatttttac ctcaaatctg
    19921 gtggctatat ggccaaatca gaatgggtag aagacaaggg agccttttat tatcttgacc
    19981 aagatggaaa gatgaaacga aatgcttggg taggaacttc ctatgttggt gcaacaggtg
    20041 ccaaagtaat agaagactgg gtctatgatt ctcaatacga tgcttggttt tatatcaaag
    20101 cagatggaca gcacgcagag aaagaatggc tccaaattaa agggaaggac tattatttca
    20161 aatccggtgg ttatctactg acaagtcagt ggattaatca agcttatgtg aatgctagtg
    20221 gtgccaaagt acagcaaggt tggctttttg acaaacaata ccaatcttgg ttttacatca
    20281 aagaaaatgg aaactatgct gataaagaat ggattttcga gaatggtcac tattattatc
    20341 taaaatctgg tggctacatg gcagccaatg aatggatttg ggataaggaa tcttggtttt
    20401 atctcaaatt tgatgggaaa atagctgaaa aagaatgggt ctacgattct catagtcaag
    20461 cttggtacta cttcaaatcc ggtggctaca tggcagccaa tgaatggatt tgggataagg
    20521 aatcttggtt ttacctcaag tttgatggga aaatagctga aaaagaatgg gtctacgatt
    20581 ctcatagtca agcctggtac tacttcaaat ccggtggtta catgacagcc aatgaatgga
    20641 tttgggataa ggaatcttgg ttttacctca agtctgatgg caaaatggct gaaaaagaat
    20701 gggtctacga ttctcatagt caagcttggt actacttcaa atctggtggc tacatggcga
    20761 aaaatgagac agtagatggt tatcagcttg aaagcgatgg taaatggctt ggaggaaaag
    20821 ctacaaatga aaatgctgct tactatcaag tagtgcctgt tacagccaat gtttatgatt
    20881 cagatggtga aaagctttcc tatatatcgc aaggtagtgt cgtatggcta gataaggata
    20941 gaaaaagtga tgacaagcgc ttggctatta ctatttctgg tttgtcaggc tatatgaaaa
    21001 cagaagattt acaagcgcta gatgctagta aggactttat cccttattat gagagtgatg
    21061 gccaccgttt ttatcactat gtggctcaga atgctagtat cccagtagct tctcatcttt
    21121 ctgatatgga agtaggcaag aaatattatt cggcagatgg cctgcatttt gatggtttta
    21181 agcttgagaa tcccttcctt ttcaaagatt taacagaggc tacaaactac agtgctgaag
    21241 aattggataa ggtatttagt ttgctaaaca ttaacaatag ccttttggag aacaagggcg
    21301 ctacttttaa ggaagccgaa gaacattacc atatcaatgc cctttatctc cttgcccata
    21361 gtgccctaga aagtaactgg ggaagaagta aaattgccaa agataagaat aatttctttg
    21421 gcattacagc ctatgatacg accccttacc tttctgccaa gacatttgat gatgtggata
    21481 agggaatttt aggtgcaacc aagtggatta aggaaaatta tatcgatagg ggaagaactt
    21541 tccttggaaa caaggcttct ggtatgaatg tggaatatgc ttcagaccct tattggggcg
    21601 aaaaaattgc tagtgtgatg atgaaaatca atgagaagct aggtggcaaa gattagtact
    21661 ataagtgaat atgatttgag tgaatagtaa gttaaaaatc ctgatttcaa gtaaaatcag
    21721 gattttttca tggatgcaat ttttttggag tctggtgtga cgcggagggt cttttgtcct
    21781 gtgtaagtga caaagccggg ttttccacca gttggtttat tgagtttttt gacttcaatc
    21841 atatctacct gcaccagatt cgacaggcgc ccttgagaga agtaggcagc taactctgct
    21901 gcgtctgtct tgactgcatc agatgggtca agatttcctg agatgacaac atggcttcca
    21961 ggaatgtcct tagcatggaa ccaaagttcc tccttgcggg ccattttaaa ggtcaattcc
    22021 tcattttgaa gattgtttcg tccgacatag atgatggttt tgccatcgct tgctagatat
    22081 tgttctagtt ttttgcgttt ctggattttc tcccgttgtc ttctgcggat aaaacctgtt
    22141 tgaatcaatt cttcacggat ttcagcgatt tcttccagtc cagcttggtt gaggacggtt
    22201 tctacacttt ccagatagag aatagtggct ttggtttctt caatcaaatc agtcaagtat
    22261 ttgacagctt ctttgagttt ctgataccgt ttaaaatagc gttgggcatt ctggttggga
    22321 gtcagagcct tatcaagcgc aatcatgata ggttggttgg tatagtagtt gtctaggata
    22381 acctggtctt ggtcgttagg cacttggtgg aggaaggttg tcagcaattc tcctttttga
    22441 cgaaattctt cagcgttgtc tgtcgccagt aactcttttt cctgtttttt gagtttgtgt
    22501 cggtttttct gaagttcatt ttcaacacga cgaatcagtt cactggcctg ctgtttgacg
    22561 cggtcgcgct cagccttatc cttatagtag gtgtccaaca aatcagaaag atttgcaaaa
    22621 ggctctccca cctgatttgc aaaaggaact ggactgaagg aagtctcagt caagcatggc
    22681 ttggtttcct gattgaaaaa atttcggaaa gcggaaagtt tttcactaac cagtatcctt
    22741 tccaattcat ttgccgtatc gcgtcccaga ccttgaaaga ggctttgaag attttttgct
    22801 gttagttctt gggtttgcag gatttcaaag agcttttcat ccttgatagt aaaaggattg
    22861 agagattctg tacttggcgg agcgatatag gtcgatcctg gaagtaaggt gcggtagcta
    22921 ttttgtgaaa agccgacgtg tttgataact tcgaggattt tatgactgct tttatcgacc
    22981 agtagaatat tactgtgttt ccccataatt tcgataatca aggtagcctg gatatggtct
    23041 ccaatctcgt ttttattgga aactgtaatt tccacaatac ggtcattttc cacttgctca
    23101 atcgactcaa tcagggcccc ctgcaaatac tttctcaaaa ccatgataaa ggtagaaggt
    23161 tgggctggat tttcaaaagt cgtttgggtc agctgaatgc gtccaaaaac tggatgggca
    23221 gaaaggagca ggcgatggct ttggcgattg ctgcggattt gcaagaccaa ctcttgttca
    23281 aaaggctgat tgattttctg gatgcgacca ttcactaatt cgcttcgcaa ttcctcaact
    23341 atgtggtgta aaaaaaatcc gtcaaatgac atcgttctct ccttgtgatt gtattccata
    23401 gtattatatc aaaaaggtag aataaaatca tggaaatgtg gtataataaa gccaagtaaa
    23461 gagaaacgag aagcacatgt atattgaaat ggtagatgaa actggtcaag tttcaaaaga
    23521 aatgttgcaa caaacccaag aaattttgga atttgcagcc caaaaattag gaaaagaaga
    23581 caaggagatg gcagtcactt ttgtgaccaa tgagcgtagt catgaactta atctggagta
    23641 ccgtgacacc gaccgtccga cagatgtcat cagccttgag tataaaccag aattggaaat
    23701 tgcctttgac gaagaggatt tgcttgaaaa tccagaattg gcagagatga tgtctgagtt
    23761 tgatgcctat attggggaat tgttcatctc tatcgataag gctcatgagc aggccgaaga
    23821 atatggtcac agctttgagc gtgagatggg cttcttggca gtacacggct ttttacatat
    23881 taacggctat gatcactata ctccggaaga agaagcggag atgttcggtt tacaagaaga
    23941 aattttgaca gcctatggac tcacaagaca ataaacgaaa atggaaaaat cgtgacctga
    24001 tatccagttt agaatttgcc atcacaggga tttttactgc tatcaaggaa gaacgcaata
    24061 tgcgaaaaca tgcagtgacg gctctagtgg tcatccttgc aggttttggt tttcaggtgt
    24121 cacgaatcga atggctcttt ctcctattga gcattttctt ggtggtagcc tttgaaatta
    24181 tcaattctgc tatcgaaaat gtggtggatt tggccagtca ctatcacttt tccatgctgg
    24241 ctaaaaatgc caaggatatg gcggctggcg cggtattagt ggtttctctt tttgcagcct
    24301 taacaggcgc attgattttt ctcccacgaa tctgggattt attattttaa acagtaagag
    24361 gaaattatga cttttaaatc aggctttgta gccattttag gacgtcccaa tgttgggaag
    24421 tcaacctttt taaatcacgt tatggggcaa aagattgcca tcatgagtga caaggcgcag
    24481 acaacgcgca ataaaatcat gggaatttac acgactgata aggagcaaat tgtctttatc
    24541 gacacaccag ggattcacaa acctaaaaca gctctcggag atttcatggt tgagtctgcc
    24601 tacagtaccc ttcgcgaagt ggacactgtt cttttcatgg tgcctgctga tgaagcgcgt
    24661 ggtaaggggg acgatatgat tatcgagcgt ctcaaggctg ccaaggttcc tgtgattttg
    24721 gtggtgaata aaatcgataa ggtccatcca gaccagctct tgtctcagat tgatgacttc
    24781 cgtaatcaaa tggactttaa ggaaattgtt ccaatctcag cccttcaggg aaataacgtg
    24841 tctcgtctag tggatatttt gagtgaaaat ctggatgaag gtttccaata tttcccgtct
    24901 gatcaaatca cagaccatcc agaacgtttc ttagtttcag aaatggttcg cgagaaagtc
    24961 ttgcacctaa ctcgtgaaga gattccgcat tctgtagcag tagttgttga ctctatgaaa
    25021 cgagacgaag agacagacaa ggttcacatc cgtgcaacca tcatggtcga gcgcgatagc
    25081 caaaaaggga ttatcatcgg taaaggtggc gctatgctta agaaaatcgg tagcatggcc
    25141 cgtcgtgata tcgaactcat gctaggagac aaggtcttcc tagaaacctg ggtcaaggtc
    25201 aagaaaaact ggcgcgataa aaagctagat ttggctgact ttggctataa tgaaagagaa
    25261 tactaagtag aggtaggctc atgcctgctt cttgttttta cagaaggagg acttatgcct
    25321 gaattacctg aggttgaaac cgtttgtcgt agcttagaaa aattgattat aggaaagaag
    25381 atttcgagta tagaaattcg ctaccccaag atgattaaga cggatttgga agagtttcaa
    25441 agggaattgc ctagtcagat tatcgagtca atgggacgtc gtggaaaata tttgcttttc
    25501 tgcctgacag acaaggtctt gatttcccat ttgcggatgg agggcaagta tttttattat
    25561 ccagaccaag tgcctgaacg caagcatgcc catgttttct tccggtttga agatgggggc
    25621 acgcttgttt atgaggatgt acgcaagttt ggaaccatgg aactcttggt gcctgacctt
    25681 ttagacgcct actttatttc taaaaaatta ggtcctgaac caagcgaaca agactttgat
    25741 ttacaggtct ttcaagctgc ccttgccaag tccaaaaagc ctatcaaatc ccatctccta
    25801 gaccagacct tggtagctgg acttggcaat atctatgtgg atgaggttct ctggcgagct
    25861 caggttcatc cagctagacc ttcccagact ttgacagcag aagaagcgac tgccattcat
    25921 gaccagacca ttgctgtttt gggccaggct gttgaaaaag gcggttccac cattcggacc
    25981 tataccaatg cctttgggga agatggaagc atgcaggatt ttcatcaggt ctatgataag
    26041 actggtcaag aatgtgtacg ctgtggtacc atcattgaga aaattcaact aggcggacga
    26101 ggcacccact tttgtccaaa ctgtcaaagg agggactgat gagaaaaatc atcggaatca
    26161 ctgggggaat tgcctctggt aagtcaactg tgacaaattt tctaagacag caaggctttc
    26221 aagtagtgga tgccgacgca gtcgtccacc aactacagaa acctggtggt cgtctgtttg
    26281 aggctctagt acagcacttt gggcaagaaa tcattcttga aaacggagaa ctcaatcgcc
    26341 ctctcctagc tagtctcatc ttttcaaatc ctgaagagca aaaatggtct aagcaaattc
    26401 aaggggagat tatccgtgag gaactggcta ctttgagaga acagttggct cagacagaag
    26461 agattttctt catggatatt cccctacttt ttgagcagga ctacagcgat tggtttgctg
    26521 agacttggtt ggtctatgtg gaccgagatg cccaagtaga acgcttaatg aaaagggacc
    26581 agttgtccaa ggatgaagct gagtctcgtc tggcagccca gtggccttta gaaaaaaaga
    26641 aagatttggc cagccaggtt cttgataata atggcaatca gaaccagctt cttaatcaag
    26701 tgcatatcct tcttgaggga ggtaggcaag atgacagaga ttaactggaa ggataatctg
    26761 cgcattgcct ggtttggtaa ttttctgaca ggagccagta tttctttggt tgtacccttt
    26821 atgcccatct tcgtggaaaa tctaggtgta gggagtgagc aagtcgcttt ttatgcaggc
    26881 ttagcaattt ctgtctctgc tatttccgcg gcgctctttt ctcctatttg gggtattctt
    26941 gctgacaaat acggccgaaa acccatgatg attcgggcag gtcttgctat gactatcact
    27001 atgggaggct tggcctttgt cccaaatatc tattggttaa tctttcttcg tttactaaac
    27061 ggtgtatttg caggttttgt tcctaatgca acggcactga tagccagtca ggttccaaag
    27121 gagaaatcag gctctgcctt aggtactttg tctacaggcg tagttgcagg tactctaact
    27181 ggtcccttta ttggtggctt tatcaaggaa gattttcaac cagtagccaa ggaaaaggct
    27241 attccaacaa aggaattatt tacctcggtt aaatatccct atcttttgct caatctcttt
    27301 ttaaccagtt ttgtcatcca attttcagct caatcgattg gccctatttt ggctctttat
    27361 gtacgcgact tagggcagac agagaatctt ctttttgtct ctggtttgat tgtgtccagt
    27421 atgggctttt ccagcatgat gagtgcagga gtcatgggca agctaggtga caaggtgggc
    27481 aatcatcgtc tcttggttgt cgcccagttt tattcagtca tcatctatct cctctgtgcc
    27541 aatgcctcta gcccccttca actaggactc tatcgtttcc tctttggatt gggaaccggt
    27601 gccttgattc ccggggttaa tgccctactc agcaaaatga ctcccaaagc cggcatttcg
    27661 agggtctttg ccttcaatca ggtattcttt tatctgggag gtgttgttgg tcccatggca
    27721 ggttctgcag tagcaggtca atttggctac catgctgtct tttatgcgac gagcctttgt
    27781 gttgccttta gttgtctctt taacctgatt caatttcgaa cattattaaa agtaaaggaa
    27841 atctagtgcg agtaaaaatt aatctcaaat gctcctcttg tggcagtatc aattacctaa
    27901 ccagtaaaaa ttcaaaaacc catccagaca agattgaggt tttaaagtat tgtcccaagg
    27961 aaagaaaagt aaccctacat cttgaatcta agtagatttt atggtaaaat aatagggatt
    28021 ttaaggagt
//
LOCUS       DAVKAO010000021        26896 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKAO010000021 DAVKAO010000000
VERSION     DAVKAO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.021"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..394)
                     /locus_tag="VWX16_000939"
     CDS             complement(<1..394)
                     /locus_tag="VWX16_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9158317.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADK"
     gene            418..834
                     /locus_tag="VWX16_000940"
                     /pseudo
     CDS             418..834
                     /locus_tag="VWX16_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            1012..2196
                     /locus_tag="VWX16_000941"
     CDS             1012..2196
                     /locus_tag="VWX16_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU9158318.1"
                     /translation="MKIAIAGSGYVGLSLAVLLAQHHEVKVIDVIKDKVESINNRKSP
                     IKDEAIEKYLVEKELNLEASLDPAHVYKDVEYAIIATPTNYDVDLNQFDTSSVEAAIK
                     TCMEYNDTCTIVIKSTIPEGYTKEVREKFNTDRIIFSPEFLRESKALYDNLYPSRIVV
                     GTDLDDSELTKRAWQFADLLKGGAIKEEVPILVVAFNEAEVAKLFSNTYLATRVAYFN
                     EIDTYSEVKGLNPKTIIDIVCYDPRIGSYYNNPSFGYGGYCLPKDTKQLKASFRDVPE
                     NLITAVVQSNKTRKDYIAGAILAKQPSVVGIYRLIMKSDSDNFRSSAVKGVMERLDNY
                     GKEIVIYEPTIECDTFMGYRVIKSLDEFKNISDIVVANRMHDDLRDIQEKLYTRDLFG
                     RE"
     gene            2212..3465
                     /locus_tag="VWX16_000942"
     CDS             2212..3465
                     /locus_tag="VWX16_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9158319.1"
                     /translation="MYTFILMLLDFFQNHDFHFFMLFFVFILIRWAVIYFHAVRYKSY
                     SCSVSDEKLFSSVIIPVVDEPLNLFESVLNRISRHKPSEIIVVINGPKNERLVKLCHD
                     FNEKLENNMTPIQCYYTPVPGKRNAIRVGLEHVDSQSDITVLVDSDTVWTPRTLSELL
                     KPFVCDKKIGGVTTRQKILDPERNLVTMFANLLEEIRAEGTMKAMSVTGKVGCLPGRT
                     IAFRTEILRECIHEFMNETFMGFHKEVSDDRSLTNLTLKKGYKTVMQDTSVVYTDAPT
                     SWKKFIRQQLRWAEGSQYNNLKMTPWMIRNAPLMFFIYFTDMILPMLLISFGVNIFLL
                     KILNITTIVYTASWWEIILYVLLGMIFSFGGRNFKAMSRMKWYYVFLIPVFIIVLSII
                     MCPIRLLGLMRCSDDLGWGTRNLTE"
     gene            3763..4683
                     /gene="galU"
                     /locus_tag="VWX16_000943"
     CDS             3763..4683
                     /gene="galU"
                     /locus_tag="VWX16_000943"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9158320.1"
                     /translation="MKKVKKAVIPAAGLGTRFLPATKALAKEMLPIVDRPTIHFVIEE
                     ALRSGIEDILVVTGKSKRSIEDYFDSTFELEYSLRKQGKMELLKSVNESTDIKVHFVR
                     QSSPRGLGDAVLQAKSFVGDDPFVVMLGDDLMDITDSTAVPLTRQLMDDYNATQASTI
                     AVMPVRYEDVSSYGVISPRLESSNGLYSVDAFVEKPKPEEAPSNLAIIGRYLLTPEIF
                     SILETQKPGAGNEIQLTDAIDTLNKTQSVFAREFVGKRYDVGDKFNFMKTSIDYALQH
                     PQIKESLKNYVIALGKQLEKLDDCSSSGHL"
     gene            4680..5897
                     /locus_tag="VWX16_000944"
     CDS             4680..5897
                     /locus_tag="VWX16_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9158321.1"
                     /translation="MNCIESYQKWLNVPDLPAYLKDELLSMDDKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLVESKGETAKKAGVAIAYDSRHFSPEFAFE
                     SAQVLAAHGIKSYVFESLRPTPELSFAVRHLGAFAGIMVTASHNPAPFNGYKVYGSDG
                     GQMLPADADALTDYIRAIDNPFAVALADLEEAKSTGLIEVIGETLDSAYLEEVKSVNI
                     NQDLIDQYGRDMKIVYTPLHGTGEMLARRALAQAGFESVQVVEAQAKPAPDFSTVASP
                     NPESQAAFALAEELGRQVDADVLVATDPDADRLGVEIRQADGSYWNLSGNQIGALIAK
                     YILEAHKQAGTLPKNAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEEG"
     gene            complement(5824..6381)
                     /locus_tag="VWX16_000945"
     CDS             complement(5824..6381)
                     /locus_tag="VWX16_000945"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9158322.1"
                     /translation="MIKCLFPNAQIVLDRFHIIQHLSREMMTTRIVIMKNFDKQSLPY
                     RAMKNHWRIFQKDSRKLSDKAFYSRTFRQTLTPREIVNKTLAFSDELHYYDDLYQLLL
                     FHLQEKNADHFFDLLTEAFPQLNQAFRTALSTLVRYRKYVTNTIQMPYSNSKLEAINK
                     LISLLQTQTCRYGYVFLQILEFSQR"
     gene            6381..7469
                     /locus_tag="VWX16_000946"
                     /pseudo
     CDS             6381..7469
                     /locus_tag="VWX16_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
     gene            7770..13073
                     /locus_tag="VWX16_000947"
     CDS             7770..13073
                     /locus_tag="VWX16_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9158323.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQERVKTEDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HYPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EVGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKILTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTSLVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTTTDWPDNNKSKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(13239..15398)
                     /locus_tag="VWX16_000948"
     CDS             complement(13239..15398)
                     /locus_tag="VWX16_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU9158324.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(15395..15991)
                     /gene="recU"
                     /locus_tag="VWX16_000949"
     CDS             complement(15395..15991)
                     /gene="recU"
                     /locus_tag="VWX16_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9158325.1"
                     /translation="MVNYPHKVSPQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLLAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            16057..16584
                     /locus_tag="VWX16_000950"
     CDS             16057..16584
                     /locus_tag="VWX16_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9158326.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETYGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            16654..16983
                     /gene="gpsB"
                     /locus_tag="VWX16_000951"
     CDS             16654..16983
                     /gene="gpsB"
                     /locus_tag="VWX16_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9158327.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            17004..17384
                     /gene="rnpB"
                     /locus_tag="VWX16_000952"
     ncRNA           17004..17384
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VWX16_000952"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            17469..18626
                     /locus_tag="VWX16_000953"
     CDS             17469..18626
                     /locus_tag="VWX16_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9158328.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            18639..20033
                     /gene="mapZ"
                     /locus_tag="VWX16_000954"
     CDS             18639..20033
                     /gene="mapZ"
                     /locus_tag="VWX16_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9158329.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSLSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            20109..21533
                     /gene="gndA"
                     /locus_tag="VWX16_000955"
     CDS             20109..21533
                     /gene="gndA"
                     /locus_tag="VWX16_000955"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9158330.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            21545..22234
                     /locus_tag="VWX16_000956"
     CDS             21545..22234
                     /locus_tag="VWX16_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9158331.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            22333..23355
                     /gene="cbpC"
                     /locus_tag="VWX16_000957"
     CDS             22333..23355
                     /gene="cbpC"
                     /locus_tag="VWX16_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9158332.1"
                     /translation="MKLLKKMMQVALAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYSLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDAENGDMKIGWTKVNGKWYYLNSSGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            23374..24495
                     /locus_tag="VWX16_000958"
     CDS             23374..24495
                     /locus_tag="VWX16_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9158333.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGMRLEGFPNS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDLTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGNGDM
                     KTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(24613..25845)
                     /locus_tag="VWX16_000959"
     CDS             complement(24613..25845)
                     /locus_tag="VWX16_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9158334.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(25888..26850)
                     /locus_tag="VWX16_000960"
     CDS             complement(25888..26850)
                     /locus_tag="VWX16_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9158335.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKG
                     ISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLM
                     KKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
BASE COUNT         8403 a         4638 c         6003 g         7852 t
ORIGIN      
        1 gtttgtccgc tttttgacag agggtaatcg ctagaggaca gcaaacggcc atagtagtga
       61 aaaatccagc acctaaagca gacaaaaggg ttgccatcag gtataaaatc atgtagaggg
      121 cgttagggtg ggtgcgtgtg cggtagagaa tgtgttgagc caaaacatca agagtaccgt
      181 tagttgttgc aacgttataa aagagagaga cgctaaaaat ggtaaaaaag agtgaggttg
      241 gccaaaaatg aagaagttct ttgggactta atcccatgag agtggttgcg atgaggtaag
      301 aaaaagcaat agccagcagg ccaatattga ttttggtgcg gtaaccaatt ccaatggcta
      361 gagcaatggc gctaatcatt attaaatgaa tcattgaatt gtcctttcag ttagaatata
      421 gaaagaggat agattgaagt tcgagaatac tgggtgtctt ctgatgttaa gtggttgtgt
      481 caaaaccatc ccaaatggca taaattgcgt ggaattggaa tgactcgtaa tactattgat
      541 agagatggta ttacaagtca agacgttcgc tattttatct ttaactttaa gcttgatgtg
      601 atgacctttt gccatagtgt tcgaggtcat tggtcagcag aaagtatgca ttggttattg
      661 gatgtggttt atcgtgaaga ccatcatcag accctggata aacgagctgc ttttaacctt
      721 aatcttatcc gaaaaatgtg tttatatttt ctaaaagtga tggtattttc taaaaaagac
      781 ctcagctatc gatgcaaaca acggtatatc tctgtccatt tggaagatta tttggaaaca
      841 gaggttagga aagtaattag tttaacggga tatcttttca aagctgatac taaggcacaa
      901 aaaaagtttg atattcccct tgacaataga taaaattatt atataattaa actattgctt
      961 tttaaataaa gtgagaatat taataataca gagaaagagg actgtagtaa aatgaaaatt
     1021 gccattgcag gaagtggtta tgtaggactg tctttagcgg tgctactagc tcagcatcat
     1081 gaagttaagg tcattgatgt tataaaggat aaggtagagt cgataaacaa tagaaaatct
     1141 ccaattaagg atgaggcgat tgagaaatac ttagttgaaa aagagttgaa tcttgaagcc
     1201 tccttagatc ctgcacacgt ttataaagac gtggagtatg ctattattgc tactccgact
     1261 aattatgatg tagacttaaa tcagtttgat acatcttcag ttgaagctgc tatcaagact
     1321 tgtatggaat ataatgatac ttgtacaatc gtaatcaaaa gtactattcc tgaagggtat
     1381 actaaagaag tgagggaaaa gtttaataca gatcgtatta ttttttctcc agagtttcta
     1441 cgtgaatcca aagctttata tgataatttg tatccatcta gaattgttgt aggaactgat
     1501 ttggatgatt ctgagttaac aaaaagagca tggcagtttg cagatctact taaaggtgga
     1561 gctattaagg aagaggttcc gatactggtt gttgctttta atgaagcaga ggttgcaaaa
     1621 ttgtttagta acacttactt ggcaactcgc gtagcttatt ttaatgagat agatacatat
     1681 agcgaggtaa aagggcttaa tcccaagaca attattgata ttgtttgtta tgatcctaga
     1741 attggatcat actataataa ccctagcttt ggttacggag ggtattgctt accaaaagac
     1801 acaaagcaat tgaaagcaag ttttagggat gttcctgaaa atctgattac agctgtggtg
     1861 caatctaata aaacaagaaa agattatata gctggagcta ttctagctaa acaacctagt
     1921 gttgtaggta tttatagatt aattatgaaa tctgattctg ataattttcg ttctagtgct
     1981 gttaagggag ttatggaacg tttggacaat tatggtaaag aaattgttat ttacgaacct
     2041 actattgagt gtgatacttt tatgggatac agagtaatta aatctttaga tgaatttaag
     2101 aatatttctg acattgttgt agcgaatcgt atgcacgatg atttaaggga tatacaagaa
     2161 aaactctata cacgcgattt atttggcaga gaataagggg caataatttt tatgtataca
     2221 tttattttaa tgttattgga tttttttcag aatcatgatt ttcatttctt tatgttgttt
     2281 tttgtcttta ttcttattcg ttgggcggtt atatattttc atgctgtcag atataagtcc
     2341 tacagttgta gtgtaagtga tgagaagtta tttagttctg taattatccc tgtcgtggat
     2401 gaaccactta atctttttga aagtgtactg aatagaattt ccagacataa accatccgaa
     2461 attattgtgg ttattaacgg cccaaaaaac gagagacttg taaaactttg tcatgatttt
     2521 aatgaaaaat tagaaaataa tatgactcca attcaatgtt attacactcc tgttcctggc
     2581 aagagaaatg ctatccgcgt tgggctggag catgtggatt cgcagagtga tattacagtt
     2641 ctagtagata gtgatacagt atggacgcct agaaccttga gtgagttgct gaagcctttt
     2701 gtttgcgata aaaaaatagg tggggtaacg acaagacaaa aaattcttga ccctgagcgt
     2761 aatctcgtga caatgtttgc taacttgtta gaggaaatta gggcagaagg aactatgaaa
     2821 gcaatgagtg tgactggtaa agtagggtgc ttacctggtc gaacaattgc ttttagaaca
     2881 gagattctca gagagtgtat acatgagttt atgaatgaaa ctttcatggg atttcataag
     2941 gaagtttctg atgatagaag tcttacaaat ttgactttaa aaaaaggcta taaaactgtt
     3001 atgcaggata cttctgttgt gtatacagat gctcctacaa gttggaaaaa gttcattaga
     3061 cagcaactaa ggtgggcaga aggttctcag tataacaatc taaagatgac tccttggatg
     3121 attagaaatg cccctcttat gttttttatt tattttacag atatgatttt acctatgcta
     3181 cttattagct ttggtgtgaa tatattcctg ttgaaaatat taaatataac tacaattgtt
     3241 tatacagctt catggtggga aattatttta tatgttcttt tgggaatgat ttttagcttt
     3301 ggaggaagaa actttaaagc tatgtctaga atgaagtggt attatgtatt tcttattcct
     3361 gtttttataa tcgttttgag tataattatg tgccctatta ggctattagg acttatgaga
     3421 tgttctgatg atttagggtg gggaactagg aatttaacag agtgagataa atagtagtgc
     3481 gtatatagag tatttactca gagtattaat tgatttttga aaaggaaaag tgttttttaa
     3541 tgttaagaaa gaacttgaaa tatcagatta tgacacgagc tggaacaatt ttagctatct
     3601 tgtttttcat tatattaggg attattgttg aagttttgtt ttaaggctca ttgtcatctg
     3661 tagtggctca cttcagacgt aagggtcata ttttaatgtg aaaagagtgt taaaagatta
     3721 atcacttata ttattttaat agaaatagtg taaggaattg ttatgaaaaa agtaaaaaaa
     3781 gctgttattc ctgctgcagg gctgggcaca cgatttttgc ctgccactaa agctttggca
     3841 aaagaaatgc ttccaattgt agaccgcccc acaattcatt ttgtcattga agaagcttta
     3901 cgttcgggta ttgaagatat tctagtagtt actggaaaga gtaaacgttc tattgaagat
     3961 tattttgatt caacttttga attggaatat agtcttagaa aacaaggaaa gatggaactt
     4021 cttaagtcag ttaatgaatc gactgatatt aaagtacatt tcgttcgtca aagttcacca
     4081 cgtggtcttg gtgacgctgt tctccaagcg aagtcttttg ttggtgacga tccctttgtt
     4141 gtaatgcttg gtgatgacct tatggatatc accgactcaa ctgctgtacc tttaacaaga
     4201 caattgatgg atgattacaa cgcaacacag gcttcaacta tcgcagtaat gcctgttaga
     4261 tatgaagatg tttcttctta tggtgtgatt tctcctagat tggaaagtag taatggcctc
     4321 tatagtgttg atgcttttgt agagaaacca aaaccagaag aagcgcctag caatttagct
     4381 attattggac gttatctact tactcctgag attttttcta tattagaaac ccaaaagcca
     4441 ggagcaggta atgaaattca attgacagat gctattgata cattgaataa gacacagagt
     4501 gtttttgcgc gtgaatttgt gggcaaacgt tatgatgttg gtgataagtt taattttatg
     4561 aaaacatcaa ttgattatgc tcttcaacat cctcagatta aagagagttt aaaaaattac
     4621 gttattgcac ttggtaagca attggagaag ctagatgact gttcgtcaag tggacaccta
     4681 tgaattgtat agaaagttat caaaaatggc taaatgtccc tgatcttcca gcttatttaa
     4741 aagatgaatt gctcagcatg gatgacaaaa caaaagaaga cgccttttac acaaaccttg
     4801 aattcggaac agctggtatg cgtggttata ttggtgctgg gacaaaccgt attaatatct
     4861 atgtggtgcg tcaagcaaca gaaggccttg ccaaattagt tgaatcaaaa ggcgaaacgg
     4921 ccaaaaaagc tggggttgct attgcctatg actcgcgaca tttttcacca gaattcgctt
     4981 ttgaatctgc ccaagtatta gcggcccatg gcattaaatc ttatgttttt gaaagcctac
     5041 gccctactcc tgagctgtct tttgctgttc gtcatctcgg agcatttgct ggtattatgg
     5101 taaccgccag tcataaccct gctcctttta atggttataa agtttacggt tctgatggtg
     5161 ggcaaatgct tccagctgat gctgatgctt taactgacta tattcgtgcg attgataacc
     5221 catttgctgt agcccttgct gacttagaag aagctaaatc aactggtctt attgaagtaa
     5281 ttggtgaaac tctcgattct gcctaccttg aagaggttaa aagcgttaat atcaatcaag
     5341 atttgattga ccaatacggt cgcgatatga aaattgtcta cacacctctt catggtactg
     5401 gagaaatgtt agcacgtcga gctttagcac aagctggttt cgaatctgtt caagttgtcg
     5461 aagctcaagc aaaaccagcc ccagacttct caacagttgc atcaccaaac cctgaaagtc
     5521 aagccgcctt tgccttagct gaagaactag ggcgtcaagt cgatgctgat gtattagtgg
     5581 cgactgaccc tgatgctgac cgtctcggtg ttgaaattcg tcaagctgat ggcagttatt
     5641 ggaacctttc tggtaaccaa atcggtgctc ttatcgccaa atacatttta gaagctcaca
     5701 aacaagctgg gacactccca aagaatgctg cattggcaaa atcaatagta tcaactgaat
     5761 tagtcaccaa aattgcggaa agctatggtg caaccatgtt taacgtgtta acaggtttca
     5821 aattcatcgc tgagaaaatt caagaatttg aagaaaaaca taaccatacc tacatgtttg
     5881 ggtttgaaga aggctgatga gtttattgat tgcctccagc ttggagttag aatagggcat
     5941 ctggatggta tttgtcacgt attttctgta gcgaaccagc gtgctaagag cagttctaaa
     6001 ggcttgattg agttggggaa aagcctcagt taacaagtca aagaaatggt cggcattctt
     6061 ttcttgcagg tggaaaagca ggagctggta aagatcgtca taatagtgga gctcatctga
     6121 gaaagctaag gttttattga cgatttctcg aggtgtcagt gtctgtctaa aagttcttga
     6181 gtagaaggcc ttatcagaca gttttcggct atctttttgg aaaattcgcc agtgattttt
     6241 catggcgcga tagggaagtg attgcttgtc aaaattcttc atgatgacaa ttctggttgt
     6301 catcatctca cggcttaggt gctggatgat atgaaatcta tcaaggacga tttgtgcatt
     6361 tggaaatagg catttaatca caagactcta ttacgtatct agttggtaca aatattgacc
     6421 gtcagtccta taaatataca tctaagacca gtgaagaaca aaaaacatct acgaaaaagg
     6481 ctctcttaaa caaggatttc cgtcaggcta ttgcatttgg atttgaccgt acagcctatg
     6541 cctctcagtt gaatggacaa actggagcaa gcaaaatctt acgtaatctc tttgttccac
     6601 caacatttgt tcaagcagat ggtaaaaact ttggcgatat ggtcaaagag aaattggtca
     6661 cttatgggga tgaatggaag gatgttaatc ttgcagattc tcaggatggt ctttacaatc
     6721 cagaaaaagc caaggctgaa tttgctaaag ctaaattagc cttacaagca gaaggagtcc
     6781 aattcccaat tcatttagat atgccagttg accagacagc aactacaaaa gttcagcgcg
     6841 tccaatctat gaaacaatcc ttggaagcaa ctttaggaac tgataatgtc attattgata
     6901 tccaacaact acaaaaagac gaagtaaacg atattacata ttttgctgaa aacgctgctg
     6961 gcgaagactg ggatttatca gataatgtcg gttggggtcc agactttgcc gatccatcaa
     7021 cctaccttga tatcatcaaa ccatctgtag gagaaagtac taaaacatat ttagggtttg
     7081 actcagggga agataatgta gctgctaaaa aagtaggtct atatgactac gaaaaattgg
     7141 ttactgaggc tggtgatgag gctacagatg ttgctaaacg ctatgataaa tacgctgcag
     7201 cccaagcttg gttgacagat agtgctttga ttattccaac tacatctcgt acagggcgtc
     7261 caatcttgtc taagatggta ccatttacaa taccatttgc attgtcagga aataaaggta
     7321 caagtgaacc aatcttatat aaatacttgg aacttcaaga caaggcagtc actgtagatg
     7381 aataccaaaa agctcaggaa aaatggatga aagaaaaaga agagtctaat aaaaaggctc
     7441 aagaagatct cgcaaaacat gtgaaataac tgttgcaaaa tataagaaag gatttagtat
     7501 ttcccttgaa tgctgaatcc ttttttacat ttgtaaagaa agattctaaa tgtacggacc
     7561 cccaaaagtt ggagtctctt tttgtcagaa tagagaaaat ttttgttaat tttacttgtt
     7621 tcctattgct ttctcagtta ttatttgtta tattaaaagc ataattattt tttatttatc
     7681 agggttaagc attgcacttt cagaggaagg agtatttttt aaaaagaaaa tgtaaacgct
     7741 tgctcaaaaa tgaaaggatt tagaagttta tgaataaagg attatttgaa aaacgttgta
     7801 aatatagtat tcggaaattt tcattaggtg ttgcttctgt tatgattgga gctgcattct
     7861 ttgggacaag tccggttctt gcagatagcg tgcagtctgg ttccacggcg aacttaccag
     7921 ctgatttagc tactgctctt gcaacagcaa aagagaatga tgggcgtgat tttgaagcgc
     7981 ctaaggtggg agaagaccaa ggttctccag aagttacaga tggacctaag acagaagaag
     8041 aactattagc acttgaaaaa gaaaaaccgg ctgaagaaaa accaaaagag gataaacctg
     8101 cagctgctaa acctgaaacg cctaagacgg taacccctga atggcaaacg gtagagaaaa
     8161 aagaacaaca gggaacagtc actatccgag aagaaaaagg tgtccgctac aaccaactat
     8221 cctcaactgc tcaaaatgat aacgcaggta aaccagctct gtttgaaaag aagggcttga
     8281 ccgttgatgc caatggaaat gcaactgttg atttaacctt caaagaagat tctgaaaagg
     8341 gcaaatcacg ctttggtgtc ttcttgaaat ttaaagatac caataataat gtttttgtcg
     8401 gttatgacaa ggatggctgg ttctgggagt ataaatctcc aacaactagc acttggtata
     8461 gaggtagtcg tgttgctgct cctgaaacag gatcaacaaa ccgtctctct atcactctca
     8521 agtcagacgg tcagctaaat gccagcaata acgatgtcaa tctctttgac acagtgactc
     8581 taccagctgc ggtcaatgac catcttaaaa atgagaagaa gattcttctc aaggcgggct
     8641 cttatgacga tgagcgaaca gttgttagcg ttaaaacgga taaccaagag agggtaaaaa
     8701 cagaggatac ccctgcccag aaagaaacag gtccggttgt tgacgatagc aaggtgactt
     8761 atgatacgat ccagtctaag gtacttaagg cagtaattga ccaagccttc cctcgtgtca
     8821 aggaatacac cttgaatgga catactttgc caggacaggt gcaacaattc aaccaagtct
     8881 ttatcaataa ccaccgaatc acccctgaag tcacttataa aaaaatcaat gagacaacag
     8941 cagagtactt gatgaagctt cgcgatgatg ctcacttaat caatgcggaa atgacagtac
     9001 gcttgcaagt tgtggacaat caattgcact ttgatgtgac caagattgtc aaccacaatc
     9061 aagtcactcc aggtcaaaag attgatgacg aaagaaaact actttcttct attagtttcc
     9121 tcggcaatgc tttagtctct gtttctagtg atcaaactgg tgctaagttt gatggggcaa
     9181 ccatgtcaaa caatacgcat gtcagcggag atgatcatat cgatgtaacc aatccaatga
     9241 aggatttggc taagggttac atgtatggat ttgtttctac agataagctt gctgctggtg
     9301 tttggagtaa ctctcaaaat agctatggtg gtggttcgaa tgactggact cgtttgacag
     9361 cttataaaga aacagtcgga aatgccaact atgtaggaat ccacagctct gaatggcaat
     9421 gggaaaaagc ttataagggc attgttttcc cagaatacac gaaggaactt ccaagtgcta
     9481 aggttgttat cactgaagat gccaatgcag acaagaaagt tgattggcaa gatggtgcca
     9541 ttgcttatcg tagcattatg aacaaccctc aaggttggga aaaagttaag gatatcacag
     9601 cttaccgtat cgcgatgaac tttggttctc aagcacaaaa cccattcctt atgaccttgg
     9661 atggtatcaa gaaaatcaat ctccacacag atggtcttgg gcaaggtgtt ctccttaaag
     9721 gatatggtag cgaaggccat gactctggtc acttgaacta tgctgatatt ggtaagcgta
     9781 tcggtggtgt cgaagacttc aagaccctaa ttgagaaggc taagaaatat ggagctcatc
     9841 taggtatcca cgttaacgct tcagaaactt atcctgagtc taaatacttc aatgaaaaaa
     9901 ttctccgtaa gaatccagat ggaagctata gctatggttg gaactggcta gatcaaggta
     9961 tcaacattga tgctgcctat gacctagctc atggtcgttt ggcacgttgg gaagatttga
    10021 agaaaaaact tggtgacggt ctcgacttta tctatgtgga cgtttggggt aatggtcaat
    10081 caggtgataa cggtgcctgg gctacccacg ttcttgccaa agaaattaac aaacaaggct
    10141 ggcgctttgc gatcgagtgg ggccatggtg gtgagtacga ctctaccttc catcactggg
    10201 cagctgactt gacctacggt ggctacacca ataaaggtat caacagtgcc atcacccgct
    10261 ttatccgtaa ccaccaaaaa gatgcttggg ttggggacta cagaagttat ggtggtgcag
    10321 ccaactatcc actgctaggt ggctacagca tgaaagactt tgaaggctgg caaggacgga
    10381 gcgactacaa tggctacgtg actaacctct ttgcccatga tgtcatgacc aagtatttcc
    10441 aacacttcac tgtaagtaaa tgggaaaatg gtacaccggt gactatgacc gataacggta
    10501 gcacctataa atggactcca gaaatgcgag tggaattggt agatgctgac aataataaag
    10561 tagttgtaac tcgtaagtca aatgatgtca atagcccaca atatcgcgaa cgtacagtaa
    10621 ctctcaacgg acgtgtcatc caagatggtt cagcttactt gactccttgg aactgggatg
    10681 caaatggtaa gaaactttct actgagaagg aaaagatgta ctacttcaat acgcaggccg
    10741 gtgcaacaac ttggaccctt ccaagcgatt gggcaaagag caaggtttac ctttacaagc
    10801 taactgacca aggtaagaca gaagagcaag aactaactgt aaaagatggt aaaattaccc
    10861 tagatcttct agcaaatcaa ccatacgttc tctatcgttc aaaacaaacc aatcctgaaa
    10921 tgtcatggag tgaaggcatg cacatctatg accaaggatt taacagtggt accttgaaac
    10981 attggaccat ttcaggcgat gcttctaagg cagaaattgt caagtctcaa ggggcaaacg
    11041 atatgcttcg tattcaagga aacaaagaaa aagttagtct cactcagaaa ttaactggct
    11101 tgaaaccaaa taccaagtat gccgtttatg tcggtgtcga taaccgtagt aatgccaagg
    11161 cgagcatcac tgtaaatact ggtgaaaaag aagtgactac ttataccaat aagtctctcg
    11221 cgctcaacta tgttaaggct tacgcccaca atacacgtcg tgacaatgct acagttaacg
    11281 atacaagtta cttccaaaac atgtacgcct tctttacaac aggatcggac gtctcaaatg
    11341 ttactctgac attgagtcgt gaagctggtg atcaagcaac ttactttgat gaaattcgta
    11401 cctttgaaaa caattcaagc atgtatggag acaagcatga tacaggtaaa ggcaccttca
    11461 agcaagactt tgaaaatgtt gctcagggta tcttcccatt tgtagtgggt ggtgttgaag
    11521 gtgttgaaga taaccgcact cacttgtctg aaaaacacta tccatataca caacgtggtt
    11581 ggaatggtaa gaaagtcgat gatgttatcg aaggaaattg gtcactcaag acaaatggac
    11641 tagtgagccg tcgtaacttg gtttaccaaa ctattccgca aaacttccgt tttgaagcag
    11701 gtaagaccta ccgtgtaacc tttgaatacg aagtaggttc agacaataca tatgcttttg
    11761 tagtcggtaa gggagaattc cagtcaggtc gtcgtggcac tcaagcaagc aacttggaaa
    11821 tgcatgaatt gccaaatact tggacagatt ctaagaaagc caagaaggca accttccttg
    11881 tgacaggtgc agaaacaggg gatacttggg taggtatcta ctcaactggc aatgcaagta
    11941 atactcgtgg tgattctggt ggaaatgcca acttccgtgg ttataacgac ttcatgatgg
    12001 ataatcttca aatcgaagaa attaccctaa caggtaagat tttgacagaa aatgctctga
    12061 agaactactt gccaacggtt gccatgacta actacaccaa agagtctatg gatgctttga
    12121 aagaggcggt ctttaacctc agtcaggccg atgatgatat cagtgtggaa gaagcgcgtg
    12181 cagagattgc caagattgaa gccttgaaga atgctttggt tcagaagaaa acgtctttgg
    12241 tagcagatga ctttgcaagt cttacagctc ctgctcaggc tcaagaaggt cttgcaaatg
    12301 cctttgatgg aaacttatct agtttatggc atacatcatg gggcggagga gatgtaggca
    12361 agcctgcaac catggtcttg aaagaaccaa ctgaaatcac tggacttcgt tatgttccac
    12421 gtggatcagg ttcaaatggt aacttgcgtg atgtgaaact tgttgtgaca gatgagtctg
    12481 gcaaggagca tacctttact acaactgatt ggccagataa caataagtca aaagatattg
    12541 actttggtaa gacaatcaag gctaagaaaa ttgtccttac tggtaccaag acatacggag
    12601 atggtggaga taaataccaa tctgcagcgg aacttatctt tactcgtcca caggtagcag
    12661 aaacacctct tgacttgtca ggctatgaag cagctttggc taaggctcag aaattaacag
    12721 acaaagacaa tcaagaggaa gtagctagcg ttcaggcaag catgaaatat gcgacggata
    12781 accatctctt gacggaaaga atggtggaat actttgcaga ttatctcaac caattaaaag
    12841 attctgctac gaaaccagat gctccaactg tagagaaacc tgagtttaaa cttagctctt
    12901 tagcttccga tcaaggtaag acgccagatt ataagcaaga aatagataga ccagaaacac
    12961 ctgaacaaat cttgccagca acaggtgaga gtcaatctga cacagccctc ttcctagcag
    13021 gtgttagcct agccctatct gctctctttg tagtaaaaac gaagaaagac tagtatttag
    13081 taaaacctct taacaagatt acggaagcag tctctatctt ttccaatgag gtttatagta
    13141 cagaaaaagt ctgagaagat gtcttctcag acttttgtta agcacataaa tacaatagtg
    13201 ctatgacaaa atcacccaga aaatctgggt gataaatgtt atggttgtgc tggttgagga
    13261 ttctgatttt gttgatcagg ggttgtattt gattgttgcg tattattgtt aggattggta
    13321 gtcgtactat tatttgtgct tggagtggtt gagctagact gtgaagttga actatctgat
    13381 gatgagcttg aactttcagt tgatgggggt tgttgtggag caggtgagtt ccacgtagaa
    13441 cgagcaccat ttttaaatac gaattctcca tttctgtaga gcccctctgg tatattccaa
    13501 tcctctggat tgcttccttc agacaggtag gtcatcatag agcggtaaac tttggcagcg
    13561 accgtaaggc cattgcctac aagtggtgtc agacggttag aatagcctgt ccatacagcc
    13621 attgaatatt tacgcgtata gccagcaaat agttcgtcag gtgctacaaa ttgagaggtc
    13681 ttgatgtggt tttcaatttc ctcgtctgta tagttagagg ttcctgtttt accagcctga
    13741 gggagccaag caagataggc atttcgtcca gttccataac tcaagactgt tttcatcatg
    13801 tcggtcatca tataggctgt cgtttccttc atggcacgag ttccgacatt agagaactct
    13861 ttttcactcc catcactaaa gacgacttta tggatataca ttggtttata gtaagttcca
    13921 ccatttgcaa aggcagcgta agcagcagcc atcttttcac tacttgctcc atattttttg
    13981 tctgattcgg ttgtgttact tgaaatggca tttgagtagt gaatacttgg gtagtcgatt
    14041 cctagaccat ttaggaaagt cttggcgcgg ttgagtccga ccttgtttag agtttccacg
    14101 gctgggacgt ttcgcgattg ttgcagggcg tattgcaagg tgatgttgcc aaagtagccc
    14161 ctatcccagt tataaacagg agtatttgtc ccagggtagt tatagggctc atcgtgaacg
    14221 atagtagcag ttgaatcgta gacaccgtac tccaaggcag gagcatagtc tgtgatcggt
    14281 ttcatagttg atccccagtc gcggtttgtt tctactgctt ggttaattcc gaaggaaaca
    14341 ttacttgact gatggcgtgc tcctagctgg gcaatgactt taccgttaga aacatcaaca
    14401 atggtagaag cgacttgcaa ttcatcgtct ggataggcaa cgtattcgtc tgtattgtaa
    14461 atatcccaca gatgtttttg agcttcttgg tctacatttg tgtagacatc catcccagtt
    14521 gtgagcaggt tatagcctgt ttcttcttca acttgattga tgacttcctt gaggtaatta
    14581 tccatgtaag cagggtaatt acttgctgat ttgagacttt gtagtccatc agtaattggt
    14641 gtattgactg ctttctcata ctgttcagca gagatgtagc cttgattttt catttcagat
    14701 aagaccaagt ttcggcggtc ttgggctgct tctggatgtg aataggggtc atattggttt
    14761 ggtgcctgag gcattccagc cagcaaggct aactgaggta aacttaaatt attgaggtct
    14821 ttaccatagt agttttgagc tgctgtctgc attccatagt tcccattaga catgtagacc
    14881 ttatttatat agtaggtcaa gatttcttgc ttggttgctt tttgttctaa ctgaatcgct
    14941 aaccaagctt cctgagcctt acgagaaata gtctggtcgg aagtcgaagt tgaaaagtaa
    15001 gtcaacttaa tcaactgttg agtgagagtt gatccacctt ggagggaatt gctttgcaga
    15061 ttgcgcaaga aagctcccag gatacggatg gtatcaatcc ccctgtggtc gaagaagcga
    15121 tggtcttcga tagaaacgat tgccttaacc aaatctgtgg gaatatcatt agcttgggca
    15181 ttgacgcggc gttcagaacc caagtcagca atgagttgat ttttattgtc gtagatttta
    15241 ctagaagttg ttgcaactag tttactctcg gataggctag gagccttgct aacgtagtag
    15301 aaaaaaactc ctccgcctaa gacaatggct gcgataacca agcttaagaa gctaatgctc
    15361 agatacttga ttaggcgcag aatcgttggt ttgttcatct tgttttacca cctaataaat
    15421 gttctttgat aacattgaga taaggaattt gagggaaggc accagccttg atttcatatc
    15481 catattctcg aatatattca agtggcattg atttttgtcc cttatcttga tgatagaagc
    15541 gaatcaaatc gaatgccagc aataagtagg tttcttgctg agaagaaaag tgaagaagga
    15601 caaagcagat tccttgttgg gcaaggactt gttccatatg ctgaatctga tgtggatgaa
    15661 aatttttcat cggaatcgca cgtttttgtt ttgtttcctt gacttcaaag tcgatgtaat
    15721 atccattata aacgccagaa tagtccgtcg ttgaagcttg tcgaaaatag gcttcaacaa
    15781 tcttggcacg acttcgttgt ggatagtcca cttgtacgat ttgaatagga gttggtttct
    15841 tatgtataac agccaagccc tgagacaaat agtagtcgtt ggtagcattg atcatctttt
    15901 caaaagacat tcctcgattt gcgaaatttt tgggttgaga aagagatgtt tgtctttttt
    15961 gtggtgaaac tttatgtgga tagttgacca taattctcct tattggtaca ataacatcac
    16021 tttattatac cataatttgc acagaaaggg ttaaaaatgg ctacagcttt ggttttaggc
    16081 tattctgctt ttgatttggg tttattttct gataaggatc ctcgtcttaa attgattaaa
    16141 aaagcgatac gtaaagattt agaggctatg gcagcagatg gggtgtcttg gcttgtgttt
    16201 acagggagtt tgggttttga atattgggtt ttagaggttg ctcaggagat gaaaaccgaa
    16261 tacggtttcc agttggccac catttttgct tttgaaacct atggggaaaa ttggaatgaa
    16321 ggcaatcaga tgaaactgag tcgctttaag caggtagact ttgtcaaata tgcctatcct
    16381 cgctatgaac acaaggggca gttaagagat taccagcagt ttttgctgga aaacacgact
    16441 agttcctatc ttttttatga tgaagaaaat gaaacaaaac tagcttattt ttaccaaaag
    16501 atgaaaaatc aagaggacta ttttataaag agattaacat ttgatcaatt aaatgaactt
    16561 gctgaaaatt tttccgaaaa ttgaagcttt gaccttgatt tttgtttgcc tttttttata
    16621 taataatact agcaagcgag aatggagaga gacatggcaa gtattatttt ttcagcgaaa
    16681 gatatttttg aacaagagtt tggacgtgaa gtccgtggct ataataaagt agaagttgac
    16741 gagtttttag acgatgtcat caaggactat gaaacctatg ctgccttggt caagtcactt
    16801 cgtcaggaaa ttgcggattt gaaggaagaa ttaactcgta aaccgaaacc ttcaccagtt
    16861 caagcagaac cccttgaagc ggcaattaca agttctatga cgaattttga tattttgaaa
    16921 cgcctgaata gattggaaaa agaagttttt ggtaaacaaa ttttagataa ctcagatttt
    16981 taagtagtta tttgagatgt gcaatttttg gataatcgcg tgaggagaat tgcttctcat
    17041 gaggaaagtc catgctagca caggctgtga tgcctgtagt gtttgtgcta ggcgaaacca
    17101 taagcctagg gacgagaaat cgttacggca gttgaaatgg ctaagtcctt ggataagcca
    17161 gagtaggctt gaaagtgcca cagtgacgga gtctttctgg aaacagagag agtggaacgc
    17221 ggtaaacccc tcaagctagc aacccaaatt ttggtcgggg catggagtac gcggaaacga
    17281 acgtagtatt ctgactgcta tcagctagag ctgttagtgg tagacagatg attatcgaag
    17341 gaagtggtcc tagtcacttc tggaacaaaa catggcttat agaaaattgc atataggttg
    17401 gggctgagaa attttctcaa cctcattttt taaagtggac atatagaaag gtcttgcaag
    17461 actgtaacat gaaaaaagaa tttaatttaa ttgcaactgt ggcagcaggg cttgaggctg
    17521 tcgttggtcg tgaagtgcga gagttgggct acgattgtca ggttgaaaat ggacgtgttc
    17581 gttttcaagg agacgtgaga gctattatcg aaaccaacct ttggcttcgg gcagcagatc
    17641 gtatcaaaat tatcgtagga acgttcccag ctaagacttt tgaagagcta tttcagggag
    17701 ttttcgcttt agattgggaa aattatttac cacttggagc tcggttcccg atttcaaaag
    17761 ctaaatgtgt taagtccaaa cttcacaatg agcccagtgt tcaggctatt tctaagaaag
    17821 ctgttgtcaa gaaattgcag aaacactatg ctcgcccaga aggggttcct ctgatggaga
    17881 atggtccaga gtttaagatt gaggtctcta ttctcaaaga tgtggcaact gtcatgattg
    17941 atacgaccgg gtctagcctc tttaaacgtg gttatcgtac cgaaaaaggt ggcgctccta
    18001 tcaaggaaaa tatggcagca gccattttac aactttctaa ctggtatcca gacaagcctt
    18061 tgattgatcc gacctgtggt tcggggactt tctgtattga ggcagttatg attgctagaa
    18121 agatggcgcc aggtcttcgt cgctcttttg catttgagga atggaactgg atcagcgatc
    18181 gcttgattca agaagtgcgc acagaagcgg ctaaaaaagt agaacgtaag cttgagttgg
    18241 atatcatggg ctgtgatatt gatgctcgca tggtggaaat tgctaaggcc aatgctcagg
    18301 cagctggtgt tgcaggagat attactttta agcagatgcg cgtgcaggat ttacgttccg
    18361 ataaaatcaa tggagtaatc atttccaatc cgccttatgg tgaacgtttg tcagatgatg
    18421 caggggtgac caagctctat gctgagatgg ggcaagtatt tgcaccgctg aaaacttgga
    18481 gcaaatttat cctgactagt gatgaagctt ttgaaagcaa gtatggtagc caagcagata
    18541 agaagcgtaa gttatacaac ggaaccttga aagtggatct atatcaatat tttggtcagc
    18601 gtgtcaaacg gcaagaggta aaatagaaag ggatactcat gagtaaaaaa agacgaaatc
    18661 gtcataaaaa agaaggtcaa gaaccgcaat ttgattttga tgaagcaaaa gagctaacag
    18721 ttggtcaagc tattcgtaaa aatgaagaag tggaagcagg agtcttgcct gaggattcca
    18781 ttttggacaa gtatgttaag caacacagag atgaaattga ggcggataag tttgcgactc
    18841 gtcaatacaa aaaagaggag ttcgttgaaa ctcagagtct ggatgattta attcaagaga
    18901 tgcgtgaggc tgtagagaag tcagaagctt cttcggagga agttccatct tctgaagaca
    18961 tcttactacc cttgcctctg gacgatgagg agcaaggctt ggatcctcta ttgctagatg
    19021 atgaaaatcc aacagaaatg actgaagaag tggaagagga gcaaaacctt tctcgtctgg
    19081 atcaagagga ctcagaaaag aaaagtaaaa aaggctttat tttgaccgtt ttggcgcttg
    19141 tatcagtaat tatttgtgtc agtgcttatt atgtctaccg tcaagtggct cgttcgacca
    19201 aggaaattga aacttctcaa tcaactacag ccaatcaatc ggatgtggat gattttaata
    19261 cactttatga cgccttttac acagatagca ataaaacggc tttgaaaaat agccagtttg
    19321 ataaactgag tcaactcaaa accttgcttg ataaactgga aggtagtcgt gaacatacgc
    19381 ttgccaaatc taaatatgat agtctagcaa cgcaaatcaa ggctattcaa gatgtcaatg
    19441 cacaatttga aaaatcagct attgtggatg gtgtgttgga taccaatgcc aaagccaaat
    19501 cggatgctaa atttacagat attaaaactg gaaatacgga gcttgataaa gtgctagata
    19561 aggctatcag tcttggtaag agccagcaaa caagtacttc tagctcaagt ttaagtcaaa
    19621 ctagcagctc aagttcaagt caagcaagtt caaatacgac tagtgagcca aaaccaagta
    19681 gttcaaatga gactagaagt agtcgcagtg aagtcaatat gggtctctcg agtgcagggg
    19741 ttgctgttca aagaagtgcc agtcgtgttg cctataatca gtctgctatt gatgatagta
    19801 ataactctgc ctgggatttt gcggatggtg tcttggaaca aattctagcg acttcacgtt
    19861 cacgtggcta tatcactgga gaccaatata tccttgaacg tgtcaatatc gttaacggca
    19921 atggttatta taacctctat aagccagatg gaacctatct ctttaccctt aactgtaaga
    19981 caggctactt tgtcggaaat ggcgctggtc atgcggatga cttggactac taagcagtcg
    20041 ttacaaaatt ctttcttttc aaaagtaaaa atgataaaat aaaacaaatg aaacaagagg
    20101 agtgtcaaat gacaaaagct aactttggtg tcgtaggtat ggccgtaatg ggtcgtaacc
    20161 ttgcccttaa tattgaatca cgtggttaca cagttgctat ctacaaccgt agtaaagaaa
    20221 aaacggaaga tgtgattgct tgccatcctg aaaagaactt tgtaccaagc tatgacgttg
    20281 aaagttttgt aaactcaatc gaaaaacctc gtcgtatcat gctgatggtt caagctggac
    20341 ctggtacaga tgctactatc caagcccttc ttccacacct tgacaagggt gatatcttga
    20401 ttgacggtgg aaatactttc tacaaagata ccatccgtcg taatgaagaa ttggcaaact
    20461 caggtatcaa ctttatcggt actggagttt ctggtggtga aaaaggtgcc cttgaaggtc
    20521 cttctatcat gcctggtgga caaaaagagg cctacgaatt ggttgcggat gttcttgaag
    20581 aaatctcagc taaagcacca gaagatggca agccatgtgt gacttacatc ggtcctgatg
    20641 gagctggtca ctatgtgaaa atggttcaca atggtattga gtacggtgat atgcaattga
    20701 tcgcagaaag ctatgacttg atgcaacact tgctaggcct ttctgcagag gatatggctg
    20761 aaatctttac tgagtggaac aagggtgaat tagacagcta cttgatcgaa atcacagctg
    20821 atatcttgag ccgtaaagac gatgaaggcc aagatggacc aatcgtagac tacatccttg
    20881 atgctgcagg taacaaggga actggtaaat ggacgagcca atcatctctt gaccttggtg
    20941 taccattgtc actgattact gagtcagtgt ttgcacgcta catttcaact tacaaagaag
    21001 aacgtgtaca tgctagcaag gtgcttccaa aaccagctgc cttcaacttt gaaggagaca
    21061 aggctgaatt gattgaaaaa atccgtcaag ccctttactt ctcaaaaatc atttcatacg
    21121 cacaaggatt tgctcaattg cgtgtagcct ctaaagaaaa caactggaac ttgccatttg
    21181 cagatatcgc atctatctgg cgtgatggct gtatcatccg ttctcgtttc ttgcaaaaga
    21241 ttacagatgc ttacaaccgc gatgcagatc ttgccaacct tcttttggac gagtacttct
    21301 tggatgttac tgctaagtac caacaagcag tacgtgatat cgtagctctt gcggttcaag
    21361 caggtgtgcc agtgccaact ttctcagcag ctattactta ctttgatagc taccgttcag
    21421 ctgaccttcc agctaacttg atccaagcac aacgtgacta ctttggtgct cacacttacc
    21481 aacgtaaaga caaagaagga accttccact actcttggta tgacgaaaaa taagtaggtc
    21541 agccatgggg aaacggattt tattacttga gaaagaacga aatctagctc attttttaag
    21601 tttggaactc cagaaagagc agtatcgggt tgatctggta gaggaggggc aaaaagccct
    21661 ctccatggct cttcagacag actatgattt gattttattg aatgttaatc tgggagatat
    21721 gatggctcag gattttgcag aaaaattgag ccgaactaaa cctgcctcag tcatcatgat
    21781 tttagatcat tgggaagact tgcaagaaga gctggaagtt gttcagcgtt ttgcagtttc
    21841 atacatctat aagccagtcc ttatcgaaaa tctggtagcg cgtatttcgg cgatcttccg
    21901 aggtcgggac ttcattgatc aacactgcag tctgatgaaa gttccaagga cctaccgcaa
    21961 tcttaggata gatgttgaac atcacacggt ttatcgtggt gaagagatga ttgctctgac
    22021 acgccgtgag tatgaccttt tggcgacact tatgggaagc aagaaagtat tgactcgtga
    22081 gcaattgttg gaaagtgttt ggaagtatga aagtgcgacc gagacaaata tcgtagatgt
    22141 ctatatccgc tatctacgga gcaagcttga tgttaaaggc caaaaaagct acattaaaac
    22201 tgtgcgtggt gttggatata ccatgcaaga atagaaaagc agttgcagtt gtgtaactgc
    22261 ttttttgagg agtttttata tattgacata caatctagtt tttactacaa tcgttttagg
    22321 aggatatata aaatgaagct tttgaaaaaa atgatgcaag tcgcattagc agtctttttc
    22381 tttggtttgc tagctacaaa tacggtattt gcgaatacca caggtggccg atttgttgat
    22441 aaggataata gaaaatatta tgtaaaagat gatcataaag caatctattg gcataaaata
    22501 gacggtaaaa cttactattt tggtgatatt ggagagatgg ttgtcggttg gcaatactta
    22561 gaaattcctg gaacaggtta tcgtgataat ttattcgata accaaccagt taatgaaatt
    22621 ggccttcagg agaagtggta ctattttgga caagatggtg ctttgctaga acaaacagat
    22681 aaacaagtac tagaggcaaa aacgtctgaa aatacaggaa aagtatacgg tgaacaatat
    22741 tctctatctg ctgaaaagag aacttattat tttgataata attatgctgt aaagacaggc
    22801 tggatttatg aagagggtca ttggtattat ttaaataagc taggaaattt tggcgatgat
    22861 tcttacaatc cactaccaat tggtgaagtt gctaagggtt ggactcaaga ttttcatgtt
    22921 actattgaca ttgatagaag caaacctgct ccatggtact acctagatgc ttcaggtaag
    22981 atgcttacag attggcaaaa agtaaacgga aaatggtatt attttggctc ctctggttct
    23041 atggcaacag gttggaaata tgtacgaggc aaatggtatt acttagataa taaaaatggt
    23101 gatatgaaaa caggatggca ataccttggt aacaagtggt actacctccg ttcatcagga
    23161 gctatggtaa ctggctggta tcaagatggt tcaacttggt actatttaga tgctgaaaat
    23221 ggtgatatga aaataggttg gacaaaagta aatggaaaat ggtattatct caattcctct
    23281 ggagcaatgg ttacaggtag tcaaactatc gatggtaaag tttataactt cgcttcatct
    23341 ggtgagtgga tttaatgttg gaggatatat aaaatgaagc ttttgaaaaa aactatgcaa
    23401 gctggactaa cagtaatttt ctttggtttg ctagctacga atacagtatt tgcagataac
    23461 tctgaaggat ggcagtttgt ccaagaaaac ggtagaacct attacaaaaa gggggacctc
    23521 aaagaaacct actggcgagt gattgatggt aagtactatt attttgattc tctatctgga
    23581 gagatggttg tcggctggca atatatcccg tttccatcta aaggtagtac aattggtcct
    23641 tacccaaatg gtatgagatt agaaggtttt ccaaattcag agtggtacta cttcgataaa
    23701 aatggagtgc tacaagagtt tgttggttgg aaaacattag agattaaaac taaagacagt
    23761 gttggaagaa agtacgggga aaaacgtgaa gattcagaag ataaagaaga gaaacgttat
    23821 tatacgaact attactttaa tcaaaatcat tctttagaga caggttggct ttatgatcag
    23881 tctaactggt attatctagc taagacggaa attaatggag aaaactacct tggtggtgaa
    23941 agacgtgcgg ggtggataaa cgatgatttg acttggtact atttagatcc aacaactggt
    24001 attatgcaaa caggttggca atatctaggt aataagtggt actacctccg ttcctcagga
    24061 gcaatggcca ctggctggta tcaggaaggt accacttggt attatttaga ccagccaaat
    24121 ggcgatatga aaacaggttg gcaaaacctt gggaacaaat ggtactatct ccgttcctca
    24181 ggagcaatgg caactggttg gtatcaggaa ggtaccactt ggtattattt agaccagcca
    24241 aatggcgata tgaaaacagg ttggcaaaac cttgggaaca aatggtacta tctccgttcc
    24301 tcaggagcaa tggcaactgg ttggtatcaa gatggttcaa cttggtacta cctaaatgca
    24361 ggtaatggag atatgaagac aggttggttc caggtcaatg gcaactggta ctatgcttat
    24421 agctcaggtg ctttggcagt gaatacgacc gtagatggct attctgtcaa ctataatggc
    24481 gaatgggttc ggtaatgaca gggcgcttat aattatatta gaacactcgc ttaatcaaag
    24541 aagtgacttt gttgtacttg acctgacgat tggggagatt aaaagcatgt gaaatctaca
    24601 catgcttttt cgttagttat gagtgttgaa cttgactaac cattgacaaa agataagagc
    24661 aattaaaata agatacaaaa atatcaatgc taactgcacc gaaacgcccg atgctatgga
    24721 tagggctaat agggagagca gggacggggc gagaatgtcc actgtgctca gagcagagcc
    24781 caccgtcccc atcttgtcac tggggatatg ggcaaagaca gctgcttgta ggcgagggct
    24841 gagaatgccg ataatcgtag aactgatgaa actggtcatg aaaattacga tataggcttg
    24901 acaaaggata ctagttatta gagtcaatag ggaaatctca cagaaaacca agaggtgcgt
    24961 gaaacggata tttttaaata gattactgct ggtcatattg ccaaggatag ctccacaaga
    25021 catgatgatg gagagcaggg cgaggagttg cccaatgctc agttgtgcta gtgtctgttg
    25081 gatgaagcgg agggcaatca gccgagggga cacatccaag atggcaatca tactggtcgt
    25141 cagaaacagt agtatgacgg tatcttttaa cctcaggagc acagccagcg actgtttaag
    25201 atgttgaaaa tatttcttaa aactcagtgc tgtatgtgac atttcaattc ttttttcaac
    25261 ctcatacaag gtatgtcgaa taacatacag gcccaaaaag gcaatgacaa aagtcagagt
    25321 gttgataagg gaaatagttt gaatacttat aacattgata aggaatgcgc cagccagatt
    25381 ggcgacaata cggacaaccc tcatcagaga ctgcctgaac cccatggcat catgcaggtc
    25441 gtccttaatt acactgatgt agagggcgtt catcatgtag gcagacaggt agctcaaggt
    25501 gtcagagatg aggttgatga tgacaatcat aatgactgaa actaatgaaa agggcgacaa
    25561 gacgagaaat actgtcaaaa tagcgtagag caggattttg attttggcaa tccagagtgc
    25621 gtgtttgact ctattttctt gaaaatctcc tagaatcccg agaaagagtt gcgatagtag
    25681 gggaaccatc tctgaaatgg aaatcagtaa aatcgctagc ggagcgaaat tgtactgagc
    25741 cacataattg ataatggcga ggtagaaaat actgtcgccg aatgatgaaa aacagctgtt
    25801 tagcagcaac tgtctatata tgttgtttgt ccaaaataat ttcatacttt tctccttatg
    25861 tctgcacatc gaatgggaat ttattattta atagacacca atatacccca tgcttcctat
    25921 tccgaagtca aacaaatctg ctttaccact atctccttga aacatcagta aaccgtgatc
    25981 atctcgttca gaacatgcta aaattacctg ttggattttt ttcatcagta atttgttttg
    26041 attgtagaca gtggtttgta gtaagctagt tattccgtgg caatatcctg tcatcatttt
    26101 tagatgatgg ttaaaaacaa attcttgata tttactaata atatctttgt ttccgtcaca
    26161 atcgtacata caaagatata agattattcc ggaaattcct ttgcaccaag aaagttgtaa
    26221 atttcctaaa ttttcagtat tagaagtaac tttttctaat aattttttta attcagtatg
    26281 gaatgtatgg agatcattat agaacataga aggttctaag actttagaat aggcaaataa
    26341 agcatatgca attccacaat atccatgagc aaagctatag tctaaaatgc tttctttggc
    26401 tgtatctctt tgtaggaaat aatgataaat ttcccctata ctattgtgga tgaaaatttt
    26461 aagagtattg tcattggtaa attgatagta ttttattaat gataatagga caccagattt
    26521 tcccaatgca aagtctattt cctctagagt atcataattc tctattaatt ttttagcggt
    26581 gacgtttgct aattgtagat agtattgatt tttactgata gaaaataaat gtaggagtaa
    26641 ccatagatag cctgcatcac caaatagcag tgagtattga tattcatatg tatgtgtcgt
    26701 tctaattgtc actgccttct tgatgataga gagtatttta gaatccagtt ctggggggta
    26761 caatttattt agaggaaaca gtagtccagc tattccgtgt tggaatgata aattacttac
    26821 gaaatttcca aattctgttg aagaagtgat ccttccttca gaatttacag ttttatctag
    26881 taaaaattgt agaaca
//
LOCUS       DAVKAO010000022        24944 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKAO010000022 DAVKAO010000000
VERSION     DAVKAO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.022"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..186)
                     /gene="blpI"
                     /locus_tag="VWX16_000961"
     CDS             complement(<1..186)
                     /gene="blpI"
                     /locus_tag="VWX16_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9158336.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIM"
     gene            468..1055
                     /locus_tag="VWX16_000962"
     CDS             468..1055
                     /locus_tag="VWX16_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9158337.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1000..1725
                     /locus_tag="VWX16_000963"
     CDS             1000..1725
                     /locus_tag="VWX16_000963"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin ABC transporter"
                     /protein_id="HEU9158338.1"
                     /translation="MVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYILQQ
                     VMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSIIDA
                     LASTILSLFLDVSILILVEGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNHDV
                     MQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQTSL
                     KQGNKISSEYPYPMVWRSISHVE"
     gene            1715..2627
                     /locus_tag="VWX16_000964"
                     /pseudo
     CDS             1715..2627
                     /locus_tag="VWX16_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            2638..3999
                     /locus_tag="VWX16_000965"
     CDS             2638..3999
                     /locus_tag="VWX16_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9158339.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVISSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQASLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTSNPHQSILNRY
                     LVASQGQTQGTAEESFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKDIVHLNSEFEGKNRI
                     PTGTEIAQIFPIITDTREVLITYYVASDYLPLLDKGQTVRLKLEKIGNHGITIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            4056..4211
                     /gene="blpC"
                     /locus_tag="VWX16_000966"
     CDS             4056..4211
                     /gene="blpC"
                     /locus_tag="VWX16_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9158340.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGEWWEELLHETILSKFKITKA
                     LELPIQL"
     gene            complement(4253..5593)
                     /locus_tag="VWX16_000967"
     CDS             complement(4253..5593)
                     /locus_tag="VWX16_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9158341.1"
                     /translation="MNIAWILLYALVINGLEIVIFFKVDGIGLTFDRIFKAFLLKFLL
                     GIIFTTFQFLAVSKYLSYFIEPLFGIGLSFLLLRGLPKKILIFYGLFPMILVELFYRG
                     VSYFVLPFLGQGIVDGDGNPIFLLIMIFVCFIVLVFLKWLDYDFTRLRREFLDTGFQK
                     SLTKINWAMGAYYLVMQSLSYLEYEQGIQSTTVRHLILVFYLLFFMGGIKKLDTYLKE
                     KLQEELNQEQTLRYRDMERYSRHIEELYKEIRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLRDSSQKLQDNKYDLGRLVNIRDRALKSLLAGKFIKAREKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASAEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTNLNTTCQNQVFRQVLTVIHAE"
     gene            complement(5607..6344)
                     /locus_tag="VWX16_000968"
     CDS             complement(5607..6344)
                     /locus_tag="VWX16_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9158342.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIREQDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPSLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(6349..6687)
                     /locus_tag="VWX16_000969"
     CDS             complement(6349..6687)
                     /locus_tag="VWX16_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9158343.1"
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVIEKASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6963..7292
                     /locus_tag="VWX16_000970"
     CDS             6963..7292
                     /locus_tag="VWX16_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9158344.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDPVKTKLIL
                     NENQRAN"
     gene            complement(7460..8509)
                     /locus_tag="VWX16_000971"
     CDS             complement(7460..8509)
                     /locus_tag="VWX16_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158345.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWIFVQTLFLLLFAPLFLVMGYGLPVFLVYVLLLGVGKYFLFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKVVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSLLAQVFIEQSWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLISFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8506..9237)
                     /locus_tag="VWX16_000972"
     CDS             complement(8506..9237)
                     /locus_tag="VWX16_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158346.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9305..9715
                     /locus_tag="VWX16_000973"
     CDS             9305..9715
                     /locus_tag="VWX16_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9158347.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9725..10012
                     /locus_tag="VWX16_000974"
     CDS             9725..10012
                     /locus_tag="VWX16_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158348.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10174..10368
                     /locus_tag="VWX16_000975"
     CDS             10174..10368
                     /locus_tag="VWX16_000975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158349.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSNHTHD
                     KPTLTWFEEIFEEYHSPFHN"
     gene            complement(10690..11826)
                     /gene="dnaJ"
                     /locus_tag="VWX16_000976"
     CDS             complement(10690..11826)
                     /gene="dnaJ"
                     /locus_tag="VWX16_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9158350.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYDLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12031..12384)
                     /locus_tag="VWX16_000977"
     CDS             complement(12031..12384)
                     /locus_tag="VWX16_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158351.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDYTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(12397..14220)
                     /gene="dnaK"
                     /locus_tag="VWX16_000978"
     CDS             complement(12397..14220)
                     /gene="dnaK"
                     /locus_tag="VWX16_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9158352.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14700..15224)
                     /gene="grpE"
                     /locus_tag="VWX16_000979"
     CDS             complement(14700..15224)
                     /gene="grpE"
                     /locus_tag="VWX16_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9158353.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDDHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15251..16285)
                     /gene="hrcA"
                     /locus_tag="VWX16_000980"
     CDS             complement(15251..16285)
                     /gene="hrcA"
                     /locus_tag="VWX16_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9158354.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREEQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16449..16958)
                     /locus_tag="VWX16_000981"
     CDS             complement(16449..16958)
                     /locus_tag="VWX16_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9158355.1"
                     /translation="MNSRVEFRIFTIVDLEKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            17566..19899
                     /locus_tag="VWX16_000982"
     CDS             17566..19899
                     /locus_tag="VWX16_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9158356.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            19912..21402
                     /locus_tag="VWX16_000983"
     CDS             19912..21402
                     /locus_tag="VWX16_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9158357.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIIKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVHGNTLEKNVREYTDD
                     EKFDIIMMNPPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDG
                     FLFGEGVKTRLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYR
                     LDMPDGYKNFSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLD
                     QCGFPKEEEEILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21402..22971
                     /locus_tag="VWX16_000984"
                     /pseudo
     CDS             21402..22971
                     /locus_tag="VWX16_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            23028..23825
                     /gene="psrA"
                     /locus_tag="VWX16_000985"
     CDS             23028..23825
                     /gene="psrA"
                     /locus_tag="VWX16_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9158358.1"
                     /translation="MKDFELHLKKAGLAENTVRLYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            23836..24444
                     /locus_tag="VWX16_000986"
     CDS             23836..24444
                     /locus_tag="VWX16_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9158359.1"
                     /translation="MVKLTTCYFCDYGSYYGNKDETTSYPIYEIPKAWRYIKFASLVN
                     FRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTL
                     LMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAI
                     KGKTLNSTSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(24392..>24944)
                     /locus_tag="VWX16_000987"
     CDS             complement(24392..>24944)
                     /locus_tag="VWX16_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9158360.1"
                     /translation="NIPMNWVVIKIKDIFSINTGLSYKKGDLSINKGVRIIRGGNIKP
                     LEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGF
                     IFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAP
                     FEEQELITQKVEKLFEKVNQLWK"
BASE COUNT         8061 a         4877 c         4558 g         7448 t
ORIGIN      
        1 cataattcct gaacctacac aaataagtgt tgctgctccc ccagttatcg gaccagtcgc
       61 agcagctaat agtactgctc caatacaacc accgattgca gatcctaaat tgcctcttcc
      121 tccactaact atttcgagtt cttcattatc cataacagaa aattgttcca tcatttttgt
      181 attcatgaca aatactcctt ttttcttttt ttatttttgt cttgttgtaa ctttgataag
      241 tttagtatat catcgttttt taaaattttt catccagatc ttgaattgtc atcgaaacgt
      301 cttgaattag cttttttatt tcaagccacc tctaaatgtt taaaaaaaat aatttctaat
      361 cactttttta ccattcagga agttttaatg actattcaag atttcataaa atatgaactt
      421 agttttatga cataatagac ctatccacta tatgaaagga attgccaatg acttcttata
      481 aacgtacatt tgttcctcaa atagatgcga gagactgtgg tgtcgctgcc ttagcctcga
      541 ttgctaaatt ctatggttca gatttttctc tagctcactt gagagaactt gcaaagacca
      601 ataaagaagg gacgactgct cttggcattg taaaagccgc tgatgaaatg ggctttgaaa
      661 caagacctgt tcaagcagat aaaacgctct ttgacatgag tgatgtcccc tatccattta
      721 tcgttcacgt taacaaagaa ggaaaactcc aacattacta tgttgtctat caaacaaaga
      781 aagactatct gattattggt gatcctgacc cttctgtaaa aatcactaaa atgtcaaaag
      841 aacgcttttt ctatgaatgg actggagtag ctatttttct agctaccaaa cccagctatc
      901 aaccccataa agataaaaag aatggtctac taagcaagct tccttcctct gattttcaaa
      961 caaaaatctc tcattgctta cattgttctc tcaagcttat tggtcactat tatcaatata
     1021 ggtggttctt actatctcca aggaatcttg gatgaataca ttccaaatca gatgaaatca
     1081 actttaggaa tcatctcagt tggtctggtt atcacctata tcctccaaca agtcatgagc
     1141 ttctccagag attatctcct aaccgttctg agtcagagat taagtattga tgtgatttta
     1201 tcctatattc gccatatttt tgaacttccc atgtctttct ttgcgacacg tcgtacagga
     1261 gaaatcattt cacggttcac agatgctaac tctattatag atgccttggc ttctaccatt
     1321 ctttctcttt ttctggatgt ttctattctg attcttgtag agggagtctt gctggcacaa
     1381 aaccctaatc tcttccttct ttctcttatt tccattccta tatacatgtt catcatcttt
     1441 tcttttatga agcctttcga aaaaatgaac catgatgtca tgcaaagtaa ttctatggtt
     1501 agctctgcca ttatcgaaga tatcaacggg attgaaacta taaagtcgct cacgagtgaa
     1561 gaaaatcgct atcaaaatat agacagcgaa tttgtagatt atttggaaaa atcctttaag
     1621 ctcagtaaat attctatttt acaaacgagt ttaaagcagg ggaacaaaat tagttctgaa
     1681 tatccttatc ctatggtttg gcgctcaatt agtcatgtcg agtaaaattt ctatcggtca
     1741 gctgattacc tttaacacac ttctttctta ctttacaact cctatggaaa atattatcaa
     1801 cctccaaacc aaactccaat ctgcgaaggt cgctaataac cgtttgaacg aagtctatct
     1861 agtcgaatct gaatttcaag ttcaagaaaa ccctgttcat tcacattttt tgatgggcga
     1921 tattgaattt gatgaccttt cttataagta tggttttgga cgagatacct taacagatat
     1981 taatctcacg attaaacaag gagataaggt tagcctagtt ggagttagtg gttctggtaa
     2041 aacaacttta gccaaaatga ttgtcaattt ctttgaaccc tacaaagggc atatttccat
     2101 caatcatcag gatattaaaa acattgataa aaaaagtctt gcgccgtcat attaattacc
     2161 taccccaaca agcctatatc tttaatggct ctattttgga aaacttaacc ttgggcggta
     2221 atcatatgat tagtcaagaa gatattctaa aagcttgtga agtagctgaa atccgtcaag
     2281 acattgaaag aatgcctatg ggctatcaaa ctcagctctc tgatggagct ggtctatcag
     2341 gaggacagaa gcaacgaatc gctctcgctc gtgctctttt aactaaatct cctgttttaa
     2401 tactagatga agctactagc ggtcttgatg tcttgactga gaaaaaagtt atagataatc
     2461 ttatgtccct aactgataaa accattctct ttgtagccca tcgtctcagt atagccgaac
     2521 gaaccaaccg tgtcattgtt cttgaccagg ggaaaatcat tgaagttggt atccaccaag
     2581 agttaatgca agcgcaaggt ttctaccatc atctattcaa taaataagga gaatgtcatg
     2641 aatcctaatc tttttagaag cgtcgagttt tatcagagac gttaccataa ctatgcgaca
     2701 gtgttaatta tacctctttc attactattt actttcatct tgattttctc ccttgttgcc
     2761 acaaaagaaa ttactgtcac ttcccaagga gaaatcgccc ctacaagtgt catttcatct
     2821 attcagtcaa ccagtgataa tcctatcctt gctaatcatt tagtggcaaa tcaagtagtt
     2881 gaaaaagggg acttactcat caaatactct gaaacaatgg aagaaagtca gaaaactgcc
     2941 ttagcaactc aattacaaag acttgagaag caaaaagaag gacttggaat tttgaaacaa
     3001 agcttagaaa aagcgactga tcttttttct ggcgaggatg aatttggcta ccataatacc
     3061 tttatgaatt ttactaaaca atcccatgat attgaactgg gtatcacaaa gactaacact
     3121 gaggtttcaa atcaagctag tcttgccaat agcagttcat cagctattga acaagaaatt
     3181 acaaaagttc aacaacaaat tggagaatat caagagctga gagatgctat cataaataac
     3241 agagcacgct taccaactag caatccacac cagtcaattt tgaatcgtta tcttgtagcc
     3301 tcacaaggac aaacacaagg aacggcagag gagtcatttt tatctcaaat taatcaaagt
     3361 attgcaggtc ttgaatcatc tatcgcaagc ctcaaaattc agcaagctgg tatcggaagt
     3421 gtagcaactt atgataatag tttagcaacc aaaattgaag tactccgcac tcaattttta
     3481 caaacagctt ctcagcaaca actaaccgtg gagaaccaat taacagaatt aaaagtacaa
     3541 ctagatcaag cgacacagcg tttagaaaac aataccttaa ccgctccaag taaagatatc
     3601 gttcatctga acagcgaatt tgaaggtaaa aatagaattc caactggtac agaaattgct
     3661 caaatattcc ctatcatcac agatacaaga gaagtactaa tcacttacta cgtagcttct
     3721 gactatctac ctctactaga taaaggacaa actgtaagat taaaactgga gaagattgga
     3781 aatcacggca ttaccatcat cggccaactt cagacaattg atcaaactcc taccagaaca
     3841 gagcaaggca atctctttaa attaaccgct cttgcaaaac tatctaatga ggatagtaaa
     3901 ctcatccaat atggcttaca aggtcgcgtc actagtgtaa ctgcaaagaa aacatatttt
     3961 gattatttca aagataaaat tttaacccat tctgattaat tttcagataa cactctataa
     4021 ctatttatta tcttatcaaa aaggagaatc ataacatgga taagaaacaa aacctaactt
     4081 catttcaaga actaacaact accgaactca accaaattac aggtggagaa tggtgggaag
     4141 aactcttaca tgaaacaatt ttaagtaaat ttaaaatcac aaaagcactt gaactaccta
     4201 ttcagctata aaaacaagac cgagaaacaa gaactctcgg tcttgttttt tatcattctg
     4261 catgtatcac agtaagtacc tgacgaaaga cttgattttg gcaggtagta tttagattgg
     4321 tattgggatg actttccaca attttcataa cggtatagag accaactcct ctctcctccc
     4381 ctttagaact tgctccaaag gagaagattt cagaaatatc gatgccctct tctttgatgg
     4441 agttttcaat gataaaagtc tcctgtgctc cattttttaa aaaggcgatt gaaacatgag
     4501 gttgactggc ctctgcacta gcttcaatag cattgtcaca aaggatagac acaatggtta
     4561 gaaaatcaag taggctcatc ccctcaacct gaatctcctc aggaacttca acattaaaga
     4621 caatgttctt ttctctagct tttataaatt ttccagctag gagactcttg agggcacggt
     4681 cacgaatatt caccaatctg cccaggtcat atttattgtc ctgcaatttc tgactggaat
     4741 cccttaagac cgagtcgtag atctctttta tctgctccat atcctcctct tcaatgccca
     4801 aacgtaagct ggttaagagg ttagtgtagt catggcgaaa actccgaatt tccttgtaaa
     4861 gttcctctat atgccgacta tagcgttcca tatctctgta gcgcaaggtc tgctcttggt
     4921 tcagttcctc ctgaagtttt tccttcaaat aggtatccaa tttcttgata ccccccataa
     4981 aaaagagtag gtaaaacact aggatgagat ggcgaacagt cgttgattga ataccttgtt
     5041 catattcaag gtaagataga ctttgcatca ctagataata agcccccatt gcccagttaa
     5101 tcttagtaag agacttttga aaacctgtat ctagaaactc ccttctcaat ctagtgaaat
     5161 catagtctaa ccatttcaaa aagactaaaa ctatgaagca aacgaatatc ataatcaata
     5221 aaaagatagg attgccatcc ccatctacaa ttccttgccc caaaaatgga agcacaaaat
     5281 aggaaacacc tctgtaaaag agctctacta atatcattgg gaagagacca taaaaaataa
     5341 ggattttttt aggaagccct cttaacaata agaaagatag acctataccg aacaaaggtt
     5401 ctataaaata ggacaaatat ttacttacag ccaaaaattg aaaagtcgta aaaatgatcc
     5461 ctagaagaaa tttcagaaga aaggccttaa aaattctatc gaaagtgaga ccaattccat
     5521 ctactttaaa gaaaatgaca atttctagtc cattaataac aagtgcatac aacaatatcc
     5581 aagcaatgtt cataaactct cctagctcag tgtaagttat taatagcctc agacacttcc
     5641 ctgaccttat aacgcgcgat cagacagctt ccaccattgg gaaagaaaag gagtttttct
     5701 ttcttatcca aatgcaccac atttgcagga ttgatgagaa aagagcgatg gcactgcaag
     5761 agactgggtt cctgcttaaa aacctcctct aaactcgccg taaattctag cctgtccgtc
     5821 ttggtataga gaataacacg atggggtctt ggggatgttt cgagatagta aacctctttg
     5881 aaaggatatt ggaattgggc aaattttgat ttaaagtaaa agcaatcttc cgccagactt
     5941 ttactatctt gactattggc atagaggagg gctgtctcga tacgagattc aaactcctct
     6001 gccgaaaggg ccttatcaat gtagtccaaa gctgacactt ggtagcgaaa ggacagaggc
     6061 ataaactccg agtgagtcgt cacaaagacg attagggcat aagggtcttg ttcccgaatc
     6121 tttctagcta cttccagtcc cttcatctct tcatttcgaa tctcaatatc caaaaagaat
     6181 agctgatggg cccccttctc atgtacctct gccagcagtt ggtccggctt gccaaatacc
     6241 tcaaaagagc taggaatgat atgatgtgct ttcaaaagtt tctcaatcgt cgtttcaatt
     6301 ctagtctgtt gggaaaaatc atcttctaaa acaaatattc tcatcttctt actctccctc
     6361 tttcaaccat ttttgacgta tttctctata gcgacgtctg gcatacttga cagcatattg
     6421 accttcttct ccgagaaaaa ggattctttc ttgaaaatca atcgatttta atttatcaag
     6481 attaaccaaa caatttcgat gacatctcat caaagtttcc ccacattggt tctcaagatt
     6541 acttaaattt tgaagcatat taaaactagc tttttctata acaatctgta cggtatgggc
     6601 tttagttgga tgtgtttgga tatagtagat atcgtcgatg tttactttat agactgtttt
     6661 ctgtgtctga ataatcatat gtttcattgg attctcctcc taagaaaaga ataacagaaa
     6721 aggtctagcc caagcacacc caatcctaaa cagtctttga aaactcctaa aaggattatt
     6781 ttctacaaca tcatgatgag cttacaacta ttttatcata aaattcactc agcgcaattc
     6841 aagacatttc aatgacaatt aaagatttga ataaaaaaaa agaattaaaa gtgataaact
     6901 aacagtatca aaacacagtt gccagaagta agactggcac ccagattaaa ggaggcattc
     6961 ccatgacaga tacagacccc atcaaaagag ctcatacttt gattactgac ttaaacaaag
     7021 cctaccaagc atgcaaacag gcaagcgctg acgacgtccg ctttcaggag caattaaact
     7081 ctattcttgg ttttctagcc aaggctgaaa cagtggataa tcgattcttg attgaactag
     7141 aaaaatttta ccagacttcc agtcttctca tgggactcag cgctcttgat ccagatgctc
     7201 caactcgcgc cgcttggcgg gcctatgacc gcttccactt tgacccagtc aagaccaagt
     7261 taatactcaa tgagaatcaa agagcaaact aggaagctag ccgcaagctg tacttgagta
     7321 cggtaaggcg acgctgacgt ggtttgaaga gattttcgaa gagtataagt ctctacggac
     7381 caaccattat cctgtagtaa caaaaaagaa gctgagacaa actgaactca acttcttttt
     7441 tagtgtcgta tcagcaatgt tagtcctgca tctgacgttt tacctgataa ggcaaataca
     7501 agactagtaa aaccaaacca gctcctagta aggctagaag ggctagtttt tcttggaagg
     7561 taatcaaccc aacaactaat tccacaagta aaacaaaact gatcaatcct cgaactacct
     7621 cctgtaagcc tttttccttt tgccccttgt ccagcggaaa gagttgggtc aaatactggt
     7681 agtcaaaggc atgatagagg gccagcaact ggaaaagcaa gaggtagtta aagagaacta
     7741 ccactgctgt cgcaatccaa gattgctcga taaagacctg cgccagcaaa gaaagcaaga
     7801 gcagacggag actaagggca aaaaggtctc catttcgcaa ataagaacgc agatagagat
     7861 tttgccaaat cttcccagac accttctgaa caacctttag aataaagtcc agataggcac
     7921 gacgcttgac gctgtttgaa attcccttga cctgcgtaaa gagggcaaag aaacgaagca
     7981 agacttgctt acgcttgctt tcttgggaaa tgacatagtc ccaatccagt ccagtttcag
     8041 tgaaaaattt gctggccttt tgacgaaaga ggaaatattt tcctaccccc aataaaagca
     8101 catagaccag aaaaacaggc aagccataac ccatgactaa aaacaagggc gcaaataaca
     8161 gcaagaagag ggtctgaaca aaaatccaaa agactaggga aatgccggtt tgacgcttga
     8221 gatggagctt gatttcctct tctccaacta agagaaagag cttgtctgga gcctccatat
     8281 aggtggcaat tcctccccaa agtaaaagta aaacagacgt aattcctaca aacaaaagga
     8341 taggccaatg attttcagga aaatgttgta agagttgact gtactggtag gctagaaagc
     8401 ccaacaggac aagcaggaac aagacaaagt ggtcattgag cacatagcgc agataacaaa
     8461 gacactcctt acgaaaggcc tgctttctct ttaaaaacaa gtctttcata gatcctcctc
     8521 tttggtcaga gccaagtaaa tatcattcaa actagcctca ggcatatcaa aggcttcacg
     8581 tagttgcagg agattgcctt tggcacgcac ctctcccttg tgaagaatga caaaggcatc
     8641 acacatcttt tccgccgaat ccagcacgtg ggtactcatg agaatagact tgcccttttg
     8701 cttctccact tccaaaagct gaatcaaatc agaaatagcc agcggatcaa gaccaaggaa
     8761 aggctcatcc acgatgaaaa gacttggatc caccacaaaa gcacagataa tcatgacctt
     8821 ctgcttcatc ccttttgaaa aatgaacagg gaaccagtct aatttctgtt ccaaacggaa
     8881 catttttaac aagggctcta ctcgttcgaa agccactttt tgctcaatac cgtaagccat
     8941 agcaaccgtt tcgatatgct ctctgagggt caattcctca tacagactag gcgtctcagg
     9001 aatgtagcca atctgcttgc ggtagctagt cgcatctcct tgcagagtca ggccattgat
     9061 attgatggag ccactataag gtgccaacag accgataatc tcattgatcg tcgttgattt
     9121 cccagcacca ttgagaccaa tcaaaccgac caactgccca ctttcaacag taaaggacac
     9181 atctttcaaa acaggaacat gaacatagcc acctgtcagg tttttaattt ctaacatatt
     9241 ttctccaaat ctggtataat gtagctatat tatatcaaaa ttcagtacag tagaggtaga
     9301 ttttatgtca gattgcattt tttgtaaaat catcgcaggg gaaattcctg cttcaaaagt
     9361 atatgaagat gagcaggtcc ttgcctttct tgatatctct caagtaacac taggacacac
     9421 cttggtcgtg ccaaaagaac actatcgcaa tcttttggag atggatgcta cgagcgccag
     9481 ccaactcttt gcccaagtac caaaagtagc tcaaaaagtc atgaaagtca ctaaggctgc
     9541 tggtatgaat atcatttcca actgtgaaga agtcgctggt caaacagttt ttcatactca
     9601 cgttcacctt gtgcctcgct acagtgctga cgatgacctc aagattgatt ttatcgccca
     9661 cgaaccagac tttgacaaac ttgctcaagt cgctgaaacc atcaaaaacg cttaaggagt
     9721 tgctatgaaa ttatccaacc tactgctatt tgcaggagct gcagccggaa gttatctggt
     9781 tacaaaaaat cgccaaacca tcacagatga agtcttgaat accactgacc gcgttcaagc
     9841 tatcaaggac gatgtggata ttatccaaaa cagcctgcaa atcattaacc agcaaaaaga
     9901 acttatcaag gaataccaag aagacttgac ttacaagttt aaggtcttgg aaaaggatat
     9961 ccagactaga ctagctgtga taaaagaaat gcagggaact gaagataagt aaaaagagcc
    10021 cgacggctct tttttaaaac gtaaacaagt tatagtgaat tgaatttaga atagcacatc
    10081 atagtttcta aagcattgtt agagattact ttaaaatcct cttatcaatt tgttcatatt
    10141 ctatttcaat ctactatata tatcccatca actatgcatc ataatttagg taactcatac
    10201 tcaataaaaa tcaaaaagca aactagaaag ctaggcacag actgctcaaa acaccgtttt
    10261 aaggttgtgg atagaactga cgaagtcagt aaccataccc atgacaagcc gacgctgacg
    10321 tggtttgaag aaatttttga agagtatcat tcaccatttc acaactaaat cgaagacttt
    10381 ctgccgtaat gaaaacacct gaaacagctt ggtttcagct gtccggaaac tttaagactt
    10441 aggttcaaag tttaggtatg gaacttcgaa ggaggtcgct accgtccgtc attacttagg
    10501 aaagtcttaa aaaatctata aagtaaaaag agcccgacag ctctttttgt ttatactcaa
    10561 tgaaaatcaa aaagcaaact aggaaactag ccacgggctg ctcaaaacac tgttttgagg
    10621 ttgtagatac gactcacata tatacggcaa ggcaacgctg acgtggtttg aagagatttt
    10681 cgaagagtat tattctccat caaaggcatc ttttatatgg tcaaagaagc cttttttctt
    10741 tggatttact ttcaagtcac cagcagtcgc gaattctttc aaggctactt tttggcggtc
    10801 gttcaagcct gtcggtgtta cgacattaac agtaacgtat tggtcaccaa ctgcaccgcc
    10861 acgaaggctc ggtgccccct tactacgcag gcggaatttc ttaccagtct gagttccctc
    10921 tggaataacc aattcaacat caccgtgaac agttggaata tctactgtat caccaagagc
    10981 cgcttggaca aagttgaggt tgagatcgta gaagatagtc gttccttcac gttcaaactt
    11041 atcgctagct tctacagaaa ctactacata caagtcacca taaggtccac cgttaaagcc
    11101 tgcttcacct tgaccagcga ggcgaatttg ttgacctgtt tccacaccag cagggatttt
    11161 cacatgtacg ctatgagctt gtttctcatg acctgttcca tgacaggttg tacatggata
    11221 tttgatttct tttcctcgac cgtgacagac atcacaggtt acttggcgac gcatcatacc
    11281 aagaggagtc tgcgtatcga cgttaatgac accagcgcca tgacagcgtc cacaagtgac
    11341 tggacttgtc cctggcttag caccagagcc attacatgta cgacagccag cttcacgatg
    11401 atacttaact tccttctcag ttccgaagat agcttcttca aatgtcaaat tgacacgata
    11461 ctggagatca tctccttggc gaggagcgtt tggattgcgc gaagaaccgc ctccgccgaa
    11521 gaaacttgag aaaatatcct caaaaccacc gaagccacct gccccattga aaccaccgaa
    11581 accaccagct ccaccaaaac caccattggc gcctgcagca ccatactggt cataggcagc
    11641 acgtttttgg tcgtcactca aagtctcata ggcttcttga acttccttgt acttgtcctc
    11701 agcaccaggc tccttgttga tatctgggtg atattttttg gaaagcttac gataagcctt
    11761 tttgatctcg tctgccgaag cattttttga cacccccaga cgatcataaa attcagtatt
    11821 gttcatacaa gataccaaga ccgtaaaatt cgactgaaaa ataggaaatc tgacgctgga
    11881 gcgacgctcc tagacagatt tctctttttt ccgagaattt aggtcgggtt caattccttt
    11941 cttttatatt gatgagataa tactccagcc actcggccct atcccttttt accgaaaaac
    12001 agaggctggg ttgcaacctc tgtggttaga ttattcattt gaaataaaaa tggttgtatt
    12061 atctctagta gtaatcttca aagacctttt agctaaatca aactcaaatt tattatagtg
    12121 tgggtcttgt gttttatata ggtgtaactc ctgacatata tgatccgaaa agtaaatcat
    12181 aacatcttta aacacagaag tgtagtcata tttactctta ccaacagcta cttctgtaaa
    12241 ttctttataa atgacatttc catttctttc cacagtcacc agcatttctg ttggattata
    12301 atccaaatta aataattctt caaaaaactt tatcttttca tgataatctg caatgtcaga
    12361 aagattattt ttattaacac tcataacact agccccttat ttttcagtaa actctccgtc
    12421 tacgacgtca tcgcctgcgt ttcctgttgc ttgtgcgcct tctgctcctt cttgagcttg
    12481 ttgcgctgct gcggcttgtt cgtagagttt cacagcaagg ccttgagctt tttcgttcaa
    12541 cgcttcaagt ttcgctttca tgtcgtccaa gttgttgtct tcttgagctt tcttaaggtc
    12601 atcaagggca gcttgggcag cgtcacgttc tgcgtcgaag cctttacctt cagtttcctt
    12661 gattgtcttt tcagtcgcaa agattgcttg gtctacttca ttacgaaggt ctacttcttc
    12721 tttacgtttc ttatcagctt cagcgtttgc ttcggcatct ttcatcatgc ggtcgatttc
    12781 ttcgtcagtc aaacctgagt tcgattggat gacaatagtt tgttcttttt gagttccaag
    12841 gtctttggcc ttaacagaca cgataccgtt cttgtcgatg tcaaatgtta cttcgatttg
    12901 aggaattcca cgaggtgcag ctgggatatc agtcaattgg aagcgtccaa gagtcttgtt
    12961 atctgctgcc attgggcgtt caccttgaag aacgtggata tcaacggctg gttggttgtc
    13021 tgctgctgtt gagaagactt gtgatttaga tgttgggatt gtagtgttgc gatcgataag
    13081 ttttgtaaat actccaccca ttgtttcgat accaagtgac aatggcgtta catcaagaag
    13141 gacaacgtcc ttgacatcac cagtaatcac accaccttgg atagccgcac ccatagcaac
    13201 tacttcatca gggtttactg atttgtttgg ttctttacca gtttcagctt taacagcttc
    13261 aacaacggca gggatacgag ttgaaccacc aacaaggata acttcgtcga tttctgacaa
    13321 gctcaaacct gcatctgaaa gggcttgacg aactggaact tttgtacgtt caacaaggtc
    13381 acgagtcaaa tcgtcaaatt tcgcacgagt caaagtcatt tccaagtgaa gaggtccagc
    13441 ctcacctgca gtgataaatg gcaagctgat ttgtgttgaa gttacaccag aaaggtcttt
    13501 cttcgctttt tcagccgcat ctttcaaacg ttgcattgcc atcttgtcag tagacaagtc
    13561 gataccgttt tctttcttga attctgctac caagtggtca atgatttttt ggtcaaagtc
    13621 gtcaccacca agtttgttgt cccctgcagt tgacaatacg tcgaagacac cgtcacccaa
    13681 ttcaaggata gagacgtcga atgtaccacc accaaggtca aataccaaga ttttttcttc
    13741 tttgtcagtc ttgtccaaac cataagcaag agctgctgca gttggttcgt taacaatacg
    13801 ttctacttca agaccagcaa ttttaccagc gtcttttgtt gcttgacgtt gagcgtcgtt
    13861 gaagtaagcc ggaactgtga taacagcttt ggttactttc tcaccaaggt agtcttcagc
    13921 gtagcctttc aagtattgaa ggatcatagc tgagatttct tgtggagtgt attcttttcc
    13981 atttgcagaa actttttcag aagttcccat cttagatttg atagagataa ctgtatctgg
    14041 gtttgtgact gcttgacgtt ttgcagcatc accaacgata atttctccgt ttttgaatga
    14101 gactacagat ggagttgtgc ggtttccttc tgggtttgcg atgattttgc tttcagttcc
    14161 ttcaagaact gcaactgctg agtttgttgt acctaagtca ataccgataa ttttagacat
    14221 gtgtttttct ccttgttagt taattttcta tttttctttt tgatactctt cttaagtggc
    14281 gacgccgtca gagcttgact ttgtcaatat ctttgactaa actttgagcc taaggtctca
    14341 aaatttgcgc aaatagcgcc acagcgaaga gtatcgtctt tggttcgctt cgctcactat
    14401 tacaaagcca aacatatttt atctttcttc atagtttatt gtcgtttcgg acaagttttc
    14461 tattctgtaa attgcgacac gaggagtcga aatcgatttt atttcgacga cgagttagta
    14521 aggaagctag acaaacgcca tagcgattgc cgttttctga cgagttgctt tagctaccgt
    14581 cagaattgcc gaatcgaaca cgccctaagc tctgagtaaa aaagatagat tttcttgtgt
    14641 tcatcgaaca cttcgtcaat ctcctatttt tactgcgagc tttttacggg ctttgtatct
    14701 tagttataca ccactaccat tgctgggcgt aggatgcggt catggagttt gtagcctttt
    14761 tgaaagactt gggcgatggt atctactggg tggtcatcgt ctgctgggag agtttggatg
    14821 gccatatggt agttatggtc aaattcgcca tctgctgcga tttcttcaat tccttcttct
    14881 ttcaaagcgt gaatcaagct ttcttgcacc atccccaagc ccttcttcac atcatctgtc
    14941 aaaccttcaa ctgcaagtgc acgctcaagg ttgtcaagag atggtaagat tgcttttgcc
    15001 aagtcctggc tacgataacg ttgcaagttt tgacgttctt cattggcacg gcgttggata
    15061 ttttgcattt ctgcatgagc gcgaagatat ttgttttcga actcatctgc acgttcattt
    15121 gccaagtcca actcagactt ttcaggagtt gtttcttcag ctgtttccac aacttcctct
    15181 tcttgaactt cttctacttc ttcatttttt atatcttggg ccatgttcgc ctcctttaat
    15241 gatttcaatc ttaatgtact tcataatgat tactgctgag gtagcggtaa aaatctgtca
    15301 acttcatagt caaaacacgg ttgaccacat tgacttgatt gattagctgt tggtaatcca
    15361 gattaactgg accgataatg gctagaattc caactccccg ataaggaatg aggaacttac
    15421 tactaatcac cgctaggtca gctaaacagg actcttgacc gtctgcaaca cgaacatttt
    15481 gcatctgttc ctcacgcaac ccctcacgaa tctccaaggc caccttttgc ggttggtcaa
    15541 agaactgata ggctgctaga ttggcaaaat tcaagagatg gaccttgccc gccatcacaa
    15601 tgttttcgtt gaacatttcc ttaaagatgt gttcaaagag atcgatgaca ttatccgttg
    15661 ttgtaaagta acgctggata atctgcggaa tctccgtccg aatcttgtag tgaatatcta
    15721 aaacggtgtg accgaggaaa cgttcctgaa tgatgctctt cagtttcagc aaatcctcct
    15781 gcaagaagtt ccttggaatc agaaactgac tagtaaccgt tcgcgactcg tctagggtaa
    15841 ataccgccaa ggctgtatgt tgccccaaaa caacgatatc aaaggctgtc aaacgttgcc
    15901 tgctcggctc aacatccagt gccactaccg tacagccact caggtctgtt agtaagttag
    15961 cagcctcttg cagaatatcc tccaatttga agaattcctg atcaaaggct ttgacaatct
    16021 catatacctc attttcagcc agccggtcaa aatccagtga gtgtttcaca tagtactgaa
    16081 aaccagcaac acttggcatc cgaccgcttg aagtatgagc cttctcaagc aacccttgtt
    16141 tttctagttc cgccatgtca ttacgaatgg ttgcactgct agagttaata gactcttgca
    16201 aggcttttga tccgacaggt tcgtgcgttt tggtaaagat gtcaataatc agatttaaaa
    16261 tatcctgctg acgctctgta accatctcat cactcctctc tgtctgatca attagcactc
    16321 aatgcatcca agtgctaact tatgattcta ttatacaccc ttagaagaga atgtcaagac
    16381 aaaaactcaa aaaattagca ctcttttatc aagagtgcta aaaatcaatc tgaagaccta
    16441 gaaccttgct attcatctac tcggtatttc ctttttagtc tgaaataacc atgaattaga
    16501 taagaaatca gaaaagcata taaaacaaaa ataagaaaga aagacataga aagatcctct
    16561 ctaaacaaac tcgtacaaac aacaagacca cctaagaaag tagctgtaca ggaacgaagt
    16621 ctagatcgca taacttccca tttgagcccc ttactcatat agacttgatc ctctggaagt
    16681 aaatcagaag acgatttacg aagaatcgaa caagaacctg ctcctatcaa ttcccaacct
    16741 ctatctctaa aatcttgcag ttcatgctta tattttttaa gaaatctaga atcatagata
    16801 cggtagatga catcgtctgg ttgacattgg tcaaaataga acaaaccaaa acgactagtt
    16861 ctatacctcc aacctttcaa atgcatctca tgtaaatatt cttcttcctt ttccaaatca
    16921 acaatggtga aaatccgaaa ttctactctg ctattcattg tcttacccca aaattagaaa
    16981 acatgcctgg cgttatttat tagataattc tttccacttt tgactcaatc tccaaaaaat
    17041 ataagaaatc tgaatcgcaa aaactatcaa taaaacccaa tctattatga aaatcaaaaa
    17101 cactttccaa ctgaaagaac tacctccagt gacaaacttt gagaaaaacg gtagtagagc
    17161 taaaaagaga agtaaaatag gaagcatccg cattgttaaa atccgtttgg cataaaaaaa
    17221 tctttattta aacaaaaata ttatagcaaa atttacgcca gtttttgaac ggctgatgta
    17281 gatattttat actttcaaaa tgtttaaatg tgattattta tttttgaaaa atagctcacc
    17341 agcccgactg aaagtgctta tagaatgata ataagtcgcc tgccgaaaac agcggaaaat
    17401 agcggtgtta tgcggagata atctgacgcg atgcgaaagt atattgcata cttattttca
    17461 acaatttagc agagtatttt tataagtgtg atataataga agtataattt gttctgatag
    17521 tttattttat ggagaagtag atttttagaa tgcggagggt tcaatatggt tgagtttata
    17581 aagtctaaga aagaaatgag tgaggaggat attaaagcaa atttcatcac tcctgctatt
    17641 gtatccaaag gatggaaaaa tggtgagcat atcgcttacg aagaatactt cactgatggt
    17701 cgaattgagg ttagaggaga taaggctcgt cgtaaagaag gaaaaaaatc agactattca
    17761 ctgtattacc aatttggaac tcgaattgca attgttgagg caaaggataa taaacacagc
    17821 gttcgagcag gattacaaca agctattgaa tatggagaga ttttagatgt tccatttgtt
    17881 tattcttcaa atggtgatgg ctttattgaa cacgaccgta tcacgagaga agaacgtgag
    17941 ctggagttag acgaattccc tactcgtgaa gaattatttt ctcgtatgac gaaggaaaaa
    18001 ggattgacgt acgaaattac agaagctatc tcaactccat actatacaga cgccttctca
    18061 atgaaaacgc cacgctatta tcagcaaata gctatcaacc gtactattga aacagttgcc
    18121 agaggacaaa aacgagtaat gtttgtgatg gcaacaggaa cggggaaaac gttcatggct
    18181 tttcaaatta ttcatcgcct tcgaaaagct ggtttggcta aacgagtttt attcttagcg
    18241 gatagaaaca tcttagtaga ccaaacgatg gctgaagact ttaagccatt cgaaaaggta
    18301 atgacgaaaa ttacaccaaa acttttgact gctcctgaaa aattaaattc ttttgaaatt
    18361 tatctagggc tttatcagca actaactggt gaagatggaa ctgaaacaca ttatcaaaaa
    18421 tttgacaaag acttctttga tttaatcgta attgatgaag cgcaccgtgg ttcagctaag
    18481 gaaaacagta actggcgtaa gataattgat tatttcagtt ctgcgacaca gattgggatg
    18541 accgctactc ctaaagaaac caagaatgct tccaatacgg aatactttgg tgagccaatc
    18601 tatacttata gtttaaaaca gggaatcgag gatggttttt tggctccata tcgtgttatg
    18661 agggttaatt tagatgtgga tgtggatggt tatcgtccag aaactggaaa agttgatact
    18721 aacggacaat taatagaaga taggtactac ggcaggaaag attttgataa aaccattgtc
    18781 attgatgata gaacgcaaag agttgccaag tttgtttctg attatatgaa gcaaagcaat
    18841 gcacgatttg ataaaacaat tgttttttgt gttgatattg accatgccga gcgaatgcgt
    18901 gctgcacttg taaaagagaa tctagactta gtccaagaag actatcgtta tgtcatgcaa
    18961 gtaactggtg acaacgctga aggaaaagct caactggata actttatgga tgtcaattct
    19021 aattttcccg ctattgtaac aacgtctaaa ttattaacga caggagttaa tgctaaaaca
    19081 tgtcgtttga ttgttttaga ctctaatatc caatccatga ctgaatttaa acaaattatt
    19141 ggtcgtggca cacgtcttta tcctcaaaag gggaaagaat tttttacgat tattgatttt
    19201 cgaaatgtta ccaatttgtt tgctgaccct gattttgatg gtgatcctgt gaaggtgctg
    19261 gaaacaggtg cgaaaacagt cagtggttct acgcccggtt tcgtagatga ggaaggtgac
    19321 ccagtagaaa aatatatcgt tacagacaag caggttacca ttcttaattc tactgttcaa
    19381 gtattagatg aaaacgggaa actgattacc gaaagcctga ccgactacac tcggaagaat
    19441 atcttaggta gctacgccac tttgaacgat tttatcacag tttggcatac ggcggataaa
    19501 aagaagctta tcttagacga actttataaa aaaggagttt atctagatgc tattcgagag
    19561 tcggagggaa tatcagaaca agaaatcgat gattttgatt tactcctaaa acttgcctat
    19621 ggtcaaaaag aattaaccaa aacggaacgt atcaataaac tcaaacaaag cggatattta
    19681 tataaatata gtgaggaagc gcgtgctgtt ttggaaattt tactgaacaa atacatggat
    19741 aaaggtattg gagaactcga aagcattgaa acattaaaac ttccagaatt tcagatatat
    19801 ggtggaacct tcaaaatcat caatacttat tttggagata aaaaacgata tttacaagca
    19861 attaaagaat tggagcaaga gctatttaca gtagcttaat gaaaggaaag tatgtcaatt
    19921 acatcatttg taaaaagaat tcaagatatc actcgaaacg atgctggtgt taatggtgat
    19981 gctcaacgta ttgagcaaat gtcttggtta ttattcttaa aaatttatga tagccgtgaa
    20041 atggtttggg aattagaaga agacgagtat gagtcaatta tcccagagga attaaaatgg
    20101 cgaaattggg ctcatgctca aaatggggaa cgggtattga caggcgatga attacttgat
    20161 tttgtcaata acaagttatt caaagagttg aaagagcttg aaataacttc aaatatgcct
    20221 attcgaaaaa cgattattaa atcagctttt gaagatgcga acaactatat gaaaaatggc
    20281 gtcttgttac gccaagtcat caatgttatt gatgaagttg atttcaatag ccctgaagat
    20341 cgtcattcgt ttaatgatat ttacgaaaaa attcttaaag atattcaaaa tgctgggaac
    20401 tcaggagaat tttatacgcc acgtgcagcg actgatttta ttgccgaagt tcttgaccca
    20461 aaacttggag aatcaatggc agaccttgct tgcggaacag gaggcttctt gacttcgact
    20521 ctgaaccgtt taagtagtca acgtaaaact agtgaagata ccaaaaaata taatacagct
    20581 gtttttggta ttgaaaagaa agcatttcct catcttttag cagttacaaa tctgtttctt
    20641 cacgaaattg atgaccctaa aattgttcat ggaaatactt tggagaaaaa tgttcatgga
    20701 aatactttgg agaaaaatgt tcgtgaatat acggatgatg aaaaatttga cattattatg
    20761 atgaatccac cttttggagg gtcagaatta gaaacaataa aaaataactt tccagcagaa
    20821 ttacggagtt ctgaaacagc tgatttattt atggctgtca ttatgtatcg tttgaaagaa
    20881 aatggtcgtg ttggagttat tttacctgat ggttttctat ttggtgaagg tgtaaaaact
    20941 cgcttgaaac aaaaactggt agatgagttc aacttgcata cgattattag gttgcctcat
    21001 agtgtctttg caccgtatac aggaatccat acgaacattc ttttctttga taaaacaaag
    21061 aaaacagaag aaacttggtt ttatcgttta gatatgccag atggttataa aaatttctcg
    21121 aaaactaagc cgatgaagtc agaacacttc aatcctgttc gtgactggtg ggaaaatcgt
    21181 gaagagattc tggaaggtaa gttctacaaa tctaaatcat ttacacctag tgaattggct
    21241 gagttgaatt ataatttaga ccagtgtggc tttccaaaag aggaagagga aatcttaaat
    21301 ccctttgagt tgattcagaa ttatcaagcg gaaagagcaa ctttaaatca taagattgat
    21361 aatgtattag ctgatatttt gcagttgttg gaggacaaat aatgacacca gaacaactta
    21421 aagcaagtat tctccaaaga gcgatggaag ggaaattagt gccgcaaaat cccaatgacg
    21481 aacctgcaag tgaattatta aagagaatta aagctgaaaa agaaaaactt atcagtgaag
    21541 gaaaaatcaa acgagataaa aaggaaactg agatatttcg tggtgatgat gggaaacatt
    21601 atgggaagtt tgctgatgga agcactcaag aaattgatgt tccttatgat attcctgata
    21661 cttgggagtg ggtgaggttt tctacattgg ttgaaattgt cagaggtggc tctccacgac
    21721 caatcaaaga ttatcttact tctgaagtag atggaataaa ttggataaaa ataggtgata
    21781 ctgaaaaggg tgaaaagtat ataaataatg ttaaagaaaa aatcaaaaaa tcagggctta
    21841 acaaaactag atttgtaaaa aaaggtacat ttttgttaac taattctatg agttttggta
    21901 gaccttatat tttgaatgtt gatggtgcaa tacacgatgg atggttggct atttcgaact
    21961 atgaaaactc attaaataaa gattatctat tctatattct ttcatcaaat gtagtttatt
    22021 ctcaatttct atctctaagt tagtggagct gttgtgaaaa acttgaatag tgataaagtt
    22081 gcttctattc ttatccctct ccctccacta tccgaacaac aacgaatagt agaagcaatc
    22141 gaatcagctt tagaaaaagt agatgaatat gctgaaagtt ataatagact agaacagcta
    22201 gataaagaat ttccagataa actaaaaaaa tctattcttc aatatgctat gcaaggaaaa
    22261 ttagttgaac aagacccaaa tgatgaatca gtcgaagttt tacttgaaaa aatacgagca
    22321 gaaaaacaaa aactctttga agaaggcaag attaaaaaga aagatttgga catttctatt
    22381 gtttcccaag gagatgataa ctcttattat gaggaagtac cttgtgaaat accagaaagt
    22441 tgggagtggg tgaggttaaa cgatataacc tcctacattc aaagagggaa gtctccaaaa
    22501 tattcaaata ttcctatata ccctgtgatt gctcagaaat gtaatcaatg gtctggcttt
    22561 tctattgact tagcaagatt tattgatccc gaaacagttc atagttacca gaaagaaaga
    22621 ttactaagag atggtgattt aatgtggaat tcaacaggtc taggtacgtt aggacgatta
    22681 gctatttacc atgaaaataa aaatccttat ggctgggctg ttgcagatag ccatgttaca
    22741 gttataagag tcttatcggg agttattaat tgccatttca tctataattt tctttcctct
    22801 cctattgttc agtcagttat tgaagaaaaa gcgtcgggta gtacaaaaca aaaagaatta
    22861 ttaacgaaaa ctataaaaga atatttaatc cccctcccac cccttcctga acaatccaga
    22921 atcgttgata aaatcgaaca atttttcgcc catattgacg cactaattta gtataatagt
    22981 tttacgtgcg gtggaatttc tatcaaacat aagataggag ttttacgttg aaagattttg
    23041 aattacattt gaagaaagct ggtttagctg aaaatactgt gcgtttgtac ctttatggtg
    23101 ttcgattttt cttggagaat tacgaactca aaatggaaga tttatttgaa tacaaaggat
    23161 atttattaga caattttaaa cctaaaactg tcaatttgcg actacaagga gtaaataaat
    23221 atctggcttt tattggtcat gatgatttga aattaaaatt tgtcaaagta cagcaaaaac
    23281 cgtttttaga agatgtcatt agccatgctg attacctttt tcttaaacgt agtttgaaaa
    23341 aagatggtat tttaaaatgg cattttgtcg tttggttttt aggtgcaact ggggctcgtg
    23401 taagtgaact tattaaacta aaagtagagc atgtagaaat cggttatttt gacatttact
    23461 caaaaggtgg taaaattcgt agactgtaca ttcctaaaaa attaagaaat agttgtctta
    23521 gttggttaga atcagaaaat cgccgaagtg gttatttatt tttgaataaa tttaacgaac
    23581 caattacagc aagaggagtt gctcaacagt taaaaaatta tgctgataaa tacaaaatga
    23641 atcctaaagt aatttaccct cattctttta ggcatttatt tgctaagaat tttttagcga
    23701 agtataatga tattgccttg cttgcagatt tgatgggaca cgaaagtata gaaactactc
    23761 gaatttatct aaggaaaaca gctactgaac aacaaaatat tgtagataaa attgttaatt
    23821 ggtaaaaaat accaggtggt caaactgact acctgctatt tttgtgatta tggctcttat
    23881 tatgggaaca aagacgaaac aacctcttat cctatatatg aaatcccaaa agcatggaga
    23941 tatattaaat ttgctagcct agttaacttc agaataggaa aaactcctcc acgcagtgaa
    24001 gctacttttt ggggaactga aataccctgg gtatctatat cagatatgcc tatatctggt
    24061 tatgtaacta atacgagaga atctatttct aaattagcat taaaatcaaa gaaaatagac
    24121 atttcaccta aaggaacttt attaatgagt tttaaattat ctattggaaa agttgccata
    24181 ttggatattc ctgccactca taatgaagcc attatatcga tattccccta tgctaataaa
    24241 gaaaatatta tcagagatta cttaatgata tttttgccac ttatctctac tttaggtgat
    24301 tcaaaagatg ctatcaaagg gaaaacatta aatagtacca gtatctccga attattaatc
    24361 cctatttcta atcatgaaga gatgaaaaga atcatttcca aagttgattt actttttcaa
    24421 aaagtttctc aactttttga gtaataagtt cctgttcctc aaaaggagct aacggaatta
    24481 atagctcgct tagtgtagtt ttaggaatat tatataaagc ttgacctgat agtttagtta
    24541 ttgctttcaa ttgtttataa aataacggag aggacaagtt aaataataga aattttgaaa
    24601 taatctctga actttcgaat ggtgttaatt ggaaaataaa tccaccagcc acaacaccat
    24661 catagtcttt atcgattctt gcaaactttc caatatgttc taaagaggtt gatacaggtg
    24721 ttattagctg attatgtttt aaataaactt gctcagagga gatgaattgt gtgtcaatgt
    24781 agtaatcatt atccaacaga gaaaattcta aaggcttaat attaccacca cgtataattc
    24841 taacaccttt attaatgctt aaatcgccct tcttgtaaga aagacctgta tttattgaaa
    24901 aaatatcttt tatttttata acaacccaat tcataggtat attc
//
LOCUS       DAVKAO010000023        24480 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKAO010000023 DAVKAO010000000
VERSION     DAVKAO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.023"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(98..2356)
                     /locus_tag="VWX16_000988"
     CDS             complement(98..2356)
                     /locus_tag="VWX16_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9158361.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPNEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VWX16_000989"
     CDS             complement(2585..2815)
                     /locus_tag="VWX16_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9158362.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3005..3688
                     /locus_tag="VWX16_000990"
     CDS             3005..3688
                     /locus_tag="VWX16_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9158363.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQCS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3688..4422
                     /locus_tag="VWX16_000991"
     CDS             3688..4422
                     /locus_tag="VWX16_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9158364.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNFFPNMTVIENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4576..5433
                     /locus_tag="VWX16_000992"
     CDS             4576..5433
                     /locus_tag="VWX16_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9158365.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5575..6447
                     /locus_tag="VWX16_000993"
     CDS             5575..6447
                     /locus_tag="VWX16_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9158366.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQDRVSKDKLV"
     gene            6729..7412
                     /gene="rpiA"
                     /locus_tag="VWX16_000994"
     CDS             6729..7412
                     /gene="rpiA"
                     /locus_tag="VWX16_000994"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9158367.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7426..8637
                     /locus_tag="VWX16_000995"
     CDS             7426..8637
                     /locus_tag="VWX16_000995"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9158368.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8639..9181
                     /locus_tag="VWX16_000996"
     CDS             8639..9181
                     /locus_tag="VWX16_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9158369.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9197..10006
                     /locus_tag="VWX16_000997"
     CDS             9197..10006
                     /locus_tag="VWX16_000997"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9158370.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRTYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10503..11525
                     /locus_tag="VWX16_000998"
     CDS             10503..11525
                     /locus_tag="VWX16_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEU9158371.1"
                     /translation="MKESENDAPKLETPLREEPRLAPQTLLEASEVLENKREESKVGI
                     TEPAQDSPILAPVEETKEEAVTEKPTNTRSLTAEDLVKISKGELHLENDLIDESFYGE
                     KALDLEGDDYQDGIKNKDGKDYLGYNSHPLLADSDGDGLADGEDDNKKEWYVTDRDSL
                     LFMELAYRDDDYIEKILDHKNLFPSLYLDRQEHKLMHNELAPFWKMKKAYYTDSGLDA
                     FLFETKSDLPYLKDGTVHMLAIRGTRVNDAKDLSADFVLLGGNKLAQADDIRKVVGEL
                     AKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDFYNHVLRKVTTFSAPKVITSRTVW
                     DAKNGF"
     gene            11535..12110
                     /locus_tag="VWX16_000999"
     CDS             11535..12110
                     /locus_tag="VWX16_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9158372.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            12135..12647
                     /locus_tag="VWX16_001000"
     CDS             12135..12647
                     /locus_tag="VWX16_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9158373.1"
                     /translation="MSAQITINHKKARYFRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLIIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            13301..14011
                     /gene="deoD"
                     /locus_tag="VWX16_001001"
     CDS             13301..14011
                     /gene="deoD"
                     /locus_tag="VWX16_001001"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9158374.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(14034..14558)
                     /locus_tag="VWX16_001002"
     CDS             complement(14034..14558)
                     /locus_tag="VWX16_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9158375.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14611..14847)
                     /locus_tag="VWX16_001003"
     CDS             complement(14611..14847)
                     /locus_tag="VWX16_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9158376.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14915..15835)
                     /locus_tag="VWX16_001004"
     CDS             complement(14915..15835)
                     /locus_tag="VWX16_001004"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9158377.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            16025..16429
                     /locus_tag="VWX16_001005"
     CDS             16025..16429
                     /locus_tag="VWX16_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158378.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFICSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16645..17226
                     /locus_tag="VWX16_001006"
     CDS             16645..17226
                     /locus_tag="VWX16_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9158379.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            17223..18500
                     /locus_tag="VWX16_001007"
     CDS             17223..18500
                     /locus_tag="VWX16_001007"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9158380.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            18518..19180
                     /gene="deoC"
                     /locus_tag="VWX16_001008"
     CDS             18518..19180
                     /gene="deoC"
                     /locus_tag="VWX16_001008"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9158381.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            19167..19556
                     /locus_tag="VWX16_001009"
     CDS             19167..19556
                     /locus_tag="VWX16_001009"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9158382.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19647..20699
                     /locus_tag="VWX16_001010"
     CDS             19647..20699
                     /locus_tag="VWX16_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9158383.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20843..22378
                     /locus_tag="VWX16_001011"
     CDS             20843..22378
                     /locus_tag="VWX16_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158384.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            22371..23429
                     /locus_tag="VWX16_001012"
     CDS             22371..23429
                     /locus_tag="VWX16_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158385.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            23432..24388
                     /locus_tag="VWX16_001013"
     CDS             23432..24388
                     /locus_tag="VWX16_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158386.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         7131 a         4408 c         5578 g         7363 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctccttttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgtttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgg atcataacct aggtcaacca
      301 acttttcctt gaccttgtca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata tggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggagcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggcccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttgaccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatcggtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt cgtccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatgtc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc gacaccaggt tcaccgataa ggacaggatt attcttggtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cgcgcccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggag gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggct attgttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccattg agattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc cagcttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg aggccaactt gactagaaaa
     2881 aactaagatt tctcgctaat tctgctagtt taaagatttt taaataaatt ggataaaaaa
     2941 tttaaatttt aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt
     3001 gactatgaat ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt
     3061 gacgattctt atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc
     3121 ttttgggcaa tgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat
     3181 tttccgtggg acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa
     3241 tgctccgact attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat
     3301 tttgattatc tctatgaata gtggtgctta tgtttcggag actgttcgtg ccggaatcaa
     3361 tgcggttcca aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ctaaaaatgc
     3421 gatgcgttat gtgattttgc cacaagcagt caaaaatatc ttgccagcat tggggaacga
     3481 atttatcacc attatcaagg acagctccct cttatcagct attggggtca tggagttgtg
     3541 gaatggggct acaacagttt ctacaacaac ctatctacct ttaacaccac ttttatttgc
     3601 agcattttac tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa
     3661 acatatggga caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta
     3721 cataaatctt ttggaaagaa tgaagtattg aagggcatca acctcgagat taaaagagga
     3781 gaagttgtcg ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg
     3841 aatttgttgg aggaagcaac caaggggaag gttatctttg agggagtcga tattacggac
     3901 aagaagaatg acctgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac
     3961 ttctttccta atatgactgt gattgaaaat attaccttgt cacctatcaa gaccaaaggt
     4021 gaaagcaggg aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca
     4081 gataaggcag acgcttatcc acagagtctg tcgggtgggc agcaacaacg gattgccatc
     4141 gcgcgtgggt tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg
     4201 gatcctgaaa tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg
     4261 actatggtta tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc
     4321 tttatggcag acggtgtggt tgttgaagat gggacacctg agcagatttt tgaacaaacc
     4381 caagaacaac ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa
     4441 gacaagatta gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa
     4501 cctaggaaag aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa
     4561 gaaaagagaa gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag
     4621 gggcagttgg ctgaaaagac tgcaaaatta aaggaagaaa cagctctagt gcctggtttg
     4681 gtagtgattt tggttgggga taatccagcc agccaagtct acgttcgcaa caaggagagg
     4741 tcagctcttg cggctggttc ccgtagcgaa gtagtgcgag ttccagagac cattactcaa
     4801 gaggaattgt tagacctgat tgctaaatat aatcaggatc cagcttggca tgggattttg
     4861 gtccagttgc cattaccaaa acatatcgat gaagaggcgg ttttattagc cattgaccca
     4921 gaaaaggatg tggatggttt ccatccccta aacatgggac gtctttggtc tggacatcca
     4981 gtcatgattc cctcgacacc tgcaggaatt atggaaatgt ttcatgaata tgggattgac
     5041 ttggaaggta aaaatgcggt cgtaatcggt cgttcaaata tcgttggaaa acctatggct
     5101 cagcttcttt tggctaaaaa tgcgacagta accttggccc actcacgaac tcataatctt
     5161 gccaaggtgg ctgctaaagc agatattctt gtagtcgcaa tcggccgcgc caagtttgtg
     5221 actgctgact ttgtcaaacc aggtgcggtt gtcattgacg ttgggatgaa ccgagatgaa
     5281 aatgggaagc tctgtggaga tgttgattat gaggcagttg caccacttgc tagccatatc
     5341 acgccagtcc ctggaggtgt cggtcctatg accattacca tgctgatgga gcaaacttat
     5401 caagcagcac ttcggacatt ggatagaaaa taagagaaac attcgattaa tagagtgtat
     5461 tttctatagc tatatctaaa atggcagaaa tgaatattaa attttagata taagattaca
     5521 aaaggaggtc tgcgtctcct ttttgttgta taataaaagt gagagggaaa aaggatgaaa
     5581 gtgattaatc aaaccttact agaaaaagtt attattgaac gttctcgttc aagtcataaa
     5641 ggagattatg gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc
     5701 atggctgctt tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg
     5761 gaaaatatcc cggctctgca cagccattta cctgagccta tggccttttc tcttcaagac
     5821 cagcaattgt taaaagagca attggagaag gcagaagtta tcttgctggg gcctggttta
     5881 cgagacgatg cttctggaga aaatctagta aaacaggtct ttgttaattt aagccaaaat
     5941 cagattttga ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt
     6001 ccctcaagcc agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt
     6061 acgattgaaa agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga
     6121 acaattttgg tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat
     6181 taccagttac aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct
     6241 gggatgatcg caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca
     6301 gtagcgaccc atcttcattc agccatagcc caagaactat ctcaagaaaa ttatgtggtc
     6361 ttaccgacgg aaatcagcag gtatcttccc aaaataatga aaattatttg tcaacaagat
     6421 agggtaagca aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat
     6481 ttatgattct tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt
     6541 tgtttttgta actggaaaga gatgaacaaa tcaaatacat aaaagataaa ctcattttta
     6601 ttcgtttggt aaaaataaaa tgcatatttt taaagaaaga ttgatgaaaa atgtagattt
     6661 gatgttttta cttgaataaa aagcggtttc atagtaaaat aagaactgag aatgatggag
     6721 gtaaatcggt ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg
     6781 atggaatggt cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg
     6841 gtcgtcgtat caaggaagaa ggattgcaga ttacagctgt aacaacttct agtgtgacca
     6901 ctaaacaggc agaaggcctt aatatcccgc tcaagtctat tgaccaagta gactttgtcg
     6961 atgtgacggt tgatggcgcg gatgaagtgg atagtcagtt taatggaatc aaaggcggtg
     7021 gtggtgccct tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg
     7081 tggatgaaag caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc
     7141 agtatggtgc agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc
     7201 gtgaaaaaga cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct
     7261 tggatgtcat tgaaaatcca attgcttttg gacaagaatt ggaccatgtc gttggtgttg
     7321 tggagcatgg tttattcaac caaatggtgg ataaggtaat cgttgctgga cgagatggag
     7381 ttcagatttc aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat
     7441 cgtattcatt tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac
     7501 tttgtcaatg caggggttcc agatggagct tctgacacac tgggacacat ttcaaaaaca
     7561 gttggtttga atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact
     7621 cctcttaaga ctgtagcagc tgaaagcaat ccaactggat atgcaacaaa attagaggaa
     7681 gtatctcttg gtaaggatac tatgactgga cactgggaaa tcatgggact caacattact
     7741 gagcctttcg atactttctg gaacggattc ccagaagaaa tcctgacaaa aatcgaagaa
     7801 ttctcaggac gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc
     7861 gatgattttg gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac
     7921 cctgttttgc agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc
     7981 tgtgaatacg ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct
     8041 cgcccttatg taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct
     8101 gtatctccat tttccccaac tgttttggat aaattgaatg aggctggtat cgatacttat
     8161 gctgtgggta aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac
     8221 aacaagtcaa atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt
     8281 gaaaaaggat tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt
     8341 aatgctcacg gttaccgtga ttgcttgcat gagtttgatg aacgcttacc tgaaattatc
     8401 gcagctatga gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg
     8461 tatgcaggaa cggatcacac tcgggaatat attccattgt tggcctatag ccctgccttt
     8521 aaaggaaatg gtctcattcc agtaggacat tttgcagata tttcagcgac tgttgccgat
     8581 aactttggtg tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataagat
     8641 gacgcgctat gctttgctgg tgagaggtat caatgttggt ggtaagaata aggtcgtcat
     8701 ggcggagctt cgtcaagaat tgacaaactt gggactggaa aaggttgaga gctacatcaa
     8761 tagtggcaat attttcttta cttcgataga ttccaaagcc caattggttg aaaagctaga
     8821 gactttcttt gcagtccatt atccatttat tcagagcttt tctttactga gtctagagga
     8881 ctttgaggcg gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga
     8941 ttttctcttt tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt
     9001 agagctgaaa gatgaagtgc tttattttgg aaaacttggg attttctggg ggaaattttc
     9061 tgaagaatcc tattctaaga ctgcctatca gaagtacttg ctgaaggtgc ctttctaccg
     9121 ccacattact attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaaata
     9181 ataaaggaga cacacaatga catttttaaa caaaatccat gaaactgcta ctttcctgaa
     9241 agaaaaggga attgcagccc ctgagttcgg tctaatcctt gggtcaggac ttggagaatt
     9301 ggcagaagaa atcgaaaatc cagttgtagt agactatgct gagattccaa actggggccg
     9361 ttcaacagta gtcggtcatg ctggtaaatt ggtatatggt gaactggcag gtcgcaaggt
     9421 cttggctctt caaggtcgtt tccatttcta tgaagggaat cctctggaag tggtgacttt
     9481 cccagttcgt gtgatgaaag ttcttggatg tgaaggtgtt attgtaacca atgcagctgg
     9541 cggtatcgga tttggtcctg gtaccttgat ggctatctca gaccatatca acatgacggg
     9601 gcaaaatcca ttgatgggtg aaaacttgga tgactttggc ccacgtttcc cagatatgtc
     9661 taggacctac acaccagaat accgtgccac tgcccatgaa gtagctaaaa aacttaatat
     9721 caagcttgat gaaggtgtct atatcggagt tactggtccg acttatgaaa caccagcaga
     9781 aattcgttcc tataagacac tgggagcaga tgcagttggt atgtctacgg ttcctgaagt
     9841 tatcgtggca gcccactctg gcttgaaagt tctgggaatt tcatgtatca ctaactttgc
     9901 ggctggtttc caagaagaac tcaatcacga agaagttgta gaagtgactg aacgtgttaa
     9961 aggtgatttc aaaggcttgc ttaaagcgat tcttgctgaa ttgtaagaaa aaagatttaa
    10021 aagggggagt gcctctgtct tttcaggatt gactgcctat ccggattaaa gaagaaacag
    10081 aggaatacta tgagcttctt cctgctctta taactgaaag aagtggaaga ataggtatgt
    10141 ctgatctgat agccagcatt gtgaaagaca agattctagg atactagcat tagcttccta
    10201 gccaagtaga ctagtatgat aaggagagat gagaatgaat tgactttctg aatttcttag
    10261 tcttatcata tatagcacaa tgagatttcg cttgagtctg cttgtaaata aacgaaaaga
    10321 aagataagaa ataatgaaaa ttggtcaacg aattatgcgc tttggcataa aaaattaagt
    10381 atcggagttg tatctgttgt agtcggcttt gatttctagc tccagctgga atttcagcca
    10441 atgaagtaaa gcaagatgta acatctgaag tggtaatagg tgtgctagat tctaaggagg
    10501 aattgaaaga gtcagaaaat gatgctccaa aactagaaac tcctcttaga gaggagccaa
    10561 gactggctcc tcaaacgctt ctggaagcaa gtgaagttct tgaaaacaaa agggaagagt
    10621 caaaagtagg gataacagag ccagctcaag actcacctat tcttgctcct gtagaagaaa
    10681 ctaaagaaga agctgtgaca gaaaaaccaa caaatactcg ttctctaact gcagaagatt
    10741 tggtgaagat ttccaaaggg gaattgcatt tagaaaatga tttgattgat gaatctttct
    10801 atggtgaaaa agctcttgat ttggaagggg atgattacca ggatggcatc aaaaacaaag
    10861 atggtaagga ttatctagga tataacagtc atcccttgct agcagacagt gatggggatg
    10921 gtttggcaga tggggaagat gataataaga aagaatggta tgtcacagac cgtgattctc
    10981 ttctctttat ggagttagct tatcgagacg atgattatat tgagaaaatt ttagatcata
    11041 agaatctttt ccctagtctc tatcttgacc gtcaagaaca caaactcatg cacaatgaat
    11101 tggctccttt ctggaagatg aaaaaagcct actatacaga tagtggcttg gatgctttct
    11161 tatttgagac caagagcgac cttccttatc tcaaagatgg aacggtgcac atgttggcta
    11221 ttcgtggaac gcgagttaat gacgccaagg acttgagtgc agattttgtt ttattaggtg
    11281 gaaataaact agctcaagcg gatgatatcc gcaaggttgt tggggaatta gccaaggata
    11341 taagtattac taagttgtat atgacaggtc attctcttgg aggctaccta gctcagattg
    11401 cagcggttga agattaccaa aaatatcctg atttttataa ccatgtattg aggaaagtga
    11461 caactttcag tgctcctaaa gtcattactt ccagaactgt ttgggatgct aagaatggtt
    11521 tctgagatgt tggtttggaa agtcgtaaat tagctgttag tggaaaaatt aagcattatg
    11581 tggttgataa tgacaatgtt gtgactccct tgattcataa taatcgtgat attgttacat
    11641 ttacaggtaa ttcacgcttt aaacaccgtt ctcgtggcta ttttgaaagt ccaatgaatg
    11701 atattcctaa ctttaatatt ggtaaacaag ctaccttgga taaacatggt tatcgtgatc
    11761 cgaaattgga taaagtgcga ttctttaaga aacaggctct acctcaatct tctagtcaac
    11821 caagcgctga accaatggaa aatattgcct taggaaaaca ggttactcaa agttcgacag
    11881 ctttcggagg agatgctaga agagctgtgg atggcaaagt cgatggtaac tatggtcaca
    11941 attctgtcac tcatacaaac ttccaatcta agccttggtg gcaagtagat ttggctaaag
    12001 aagaaaccat tcgccaaatc aatatttaca accgaacaga cactgcccag gatagattgg
    12061 caaactttga tgtcattctt ttagacagtt ctggtaaaga aattgagtga aaacgtataa
    12121 tatctcctaa agatgtgtca gcacaaatta cgattaacca taaaaaagcg cgctattttc
    12181 ggattgagct agaaggctat aatgccctca gtcttgcaga agttgaagtt ttccgcttta
    12241 tagctacgaa tgctgaaacg gcgacacaag tttctaagcc agttcaacca atcagtcaga
    12301 ctcctgtgaa agataaaaca ttgataattc aacacagtgg agcttacatt gcccgctact
    12361 ccataacttg ggaagaagtt ccagtagata aagatggaaa ccaagttgtt cgtagtcatt
    12421 cttgggaagg aaacggtcgc aaccagactg caggttttgt cctcaacctc ccaatcaaag
    12481 aaaatatgag aaatctgcga gttaagattg agaaaaagac gggcctacta tggaatagat
    12541 ggcaaacaat ctatgaaaac agaccaattt tagctcaacc ccaccgtaaa attacccatt
    12601 ggggtacgac attgaattcc aaggtgagtg acgatgatgt cttgtaatct gatggtagaa
    12661 tgacagttag tttgtctagt ttataagaaa gtactacctg agcttgaata gaactcaggt
    12721 agctctctat gaaagaacaa aattaatact caatgaaaat caaagagcaa actaggaaac
    12781 tagccgcagg ttgctcaaag cactgctttg aggttgtaga taagactgac gaagtcagtc
    12841 acatatataa tccaaggcga cgttgacgtg gtttgaagag attttagaag agtataaaca
    12901 gaaaggtaga gcgcgtgttc taatttgaac acgagtagaa aacttttcta aaagcaaaaa
    12961 cgaaaggatg ggtaaactgt attcgctgaa ctgaatacgg gcgactctcc tctaaatcaa
    13021 aattaagaaa ggaattgacc ccaccctaaa agtagtggga aaaagatagt tggtctagcg
    13081 agcatcgctc actgcgccca actcctattt tcccttcgct ttttgatggg tttggtatct
    13141 ttctcaatat aaaatataaa ataaagaaag atagagcgtg tgttttgatt tgaacacgag
    13201 cggaaaactc ggaaaataga taatctgact gaaaaatcag gatttctcgt caggttccta
    13261 attttcagtc gttttcttct cgctctttgt atcataaatt atgtctatcc atattgctgc
    13321 tcagcagggt gaaattgctg ataaaattct tcttcctggg gatcctcttc gtgctaagtt
    13381 tattgcggag aatttccttg atgatgctgt ttgttttaac gaagtgcgta acatgtttgg
    13441 ttacactggt acttacaagg gtcactgtgt atctgtcatg ggaactggga tgggaatgcc
    13501 atctatttcg atttatgcgc gtgagttaat cgtagactac ggtgtgaaga aattgattcg
    13561 tgtgggaact gcaggttctt tgaatgaaga ggttcatgtt cgtgaattag ttttggcgca
    13621 ggcggctgca accaactcaa acatcgttcg taatgactgg ccacagtacg attttccaca
    13681 aattgctagc tttgatttgc ttgataaagc ttaccatatc gccaaaaaac ttggtatgac
    13741 tactcacgtt gggaacgttt tgtcatctga tgtcttttac tcaaattact ttgaaaagaa
    13801 tatcgagctt ggtaaatggg gagtcaaggc tgtggaaatg gaagcagcag ctctttacta
    13861 tcttgctgcc caataccatg ttgatgcgct agctatcatg accatctctg atagcttggt
    13921 caatccagac gaagacacaa ctgcagaaga acgtcaaaat accttcactg atatgatgaa
    13981 ggttggtttg gaaaccttga ttgcagaata attatagcca aaaaggaagc cctttatgaa
    14041 ttgtaggact tccttttctt atccagaaat tgatctagct ctctctgatt tcgaagaata
    14101 gtgactttat gtgaatattc ttggcaaagt ttttggtaat tttctttttg agttttgcta
    14161 cgcccatccc aaagaatcca tctgataaac tcccactcaa agcgttcagg gcaatctgcc
    14221 gccatacttt ctctgacttt tccacggtat ttaagataac gcttaaaggc tctaaagaga
    14281 caggtcaatg gcgaaaaatt gagaaagatg atttggtcag cttcttgcat tcgttcttgg
    14341 tagtagcacc aagaataatt accatcgatg acccaagctt tatgcttggt gagaaagttt
    14401 tttatctcgg ttaacatcca ttcgcagtca ctgtcttgcc aaccaggttg aaattggagt
    14461 gtgtccatgt gcagttttgg aatggagtag tagttagata acttttctgc tagagttgac
    14521 ttaccagaac cagaatatcc gataattgcg attttcattt tctacctttt cctatttgga
    14581 gacaaaaaaa cagcctctat ggactgtttc ttatttagca agtttagctg aaagacgagc
    14641 tttatcgcgg cttgctttgt ttttgtgaat caaaccttta gtttctgctt tatcgatagc
    14701 tgagctagca gcacggaaaa gttcttcaga tgggtttgct tcgaaagctt ttatagcagt
    14761 acgcatagct gatttttgag ctgagttctt ttcgttttgt ttaacgttca attcagcgcg
    14821 tttgatagct gatttaatgt ttgccaatgt tcttacctcc atatttacta actataccat
    14881 tatatatgaa aacttacgtt ttgacaaggg aaaattactt ttttaagtaa atttcatcga
    14941 tttcatggtt cttgctttta tgaagaatca cttctgcacg atttctggtt ggttcaatat
    15001 aattttgcag atttgtgaga ttgatactgg tccagacctg atgggcaaag gactccactt
    15061 ccccaatcgg catctgagta aaacgataat agtagctatc agggtcgttt tgggctaggc
    15121 tcagcatttt caagaaacgg tccagatacc aactttcaat atcatccact ccagcatcta
    15181 catagatgga aaagtcaaag aagtcagtga tatagagacg atcgttttgt ggattttgaa
    15241 agacattaat tccctcaaca attacaaaat cagcagcttt gacactttgt ttcttttcgg
    15301 gtacgatgtc gtaaacttca tgagaataga caggaatatc tacatcttgt ccatttttga
    15361 tgcggtccaa gaagttgaga agagcttcca tatcatagct ttcaggaaat cctttacgat
    15421 ttaaaatccc ctgctcaatc aaggtttgat tgggatagag aaaaccatca gttgtaacca
    15481 actcaaccgt agcatctgta aacgtacggg acagtaggat ttgaagtagg cgactggttg
    15541 tggattttcc aacggcaaca ctcccagaaa ccccaataat aaaaggttga gatttacttt
    15601 cacgttggag gaaaattcct tttgaaaagg ctaaatcttc cttagttcgc ttgtaaatct
    15661 gaatcagatg agccaagggg agatagatat ctgtaacgtc ttggagactg atttggtcat
    15721 taaaactctt gatagattcc aattcttctt ctgtcaaagg aggtgttgtc tttcgatgta
    15781 aagattgcca agtctggcgg ctgatttttt caaaatgtaa aaattcgttg gtcatttgtt
    15841 ttcccctaga tattttgtct atcataccat aaaaagctaa aaaaataaag cggtttcttg
    15901 aatgtggtaa gtcaatggta tatagtataa gtgatcaatt tattgatgaa ataggagata
    15961 gattctactg atttataagc ttataaaggg ggctttttag ttcacaactt ataggaggtg
    16021 tactatgaaa atcttaaaac gttacatatt ggaactctgt tttattttaa gttttgcttt
    16081 accttttata aaaggagcca atgcagataa tggtagatgc tttgtggaaa cctattacgg
    16141 ttttactttt ttgatggaac atgctattgt aacagctgtc tttatctgtt cgttcttaat
    16201 tgctttctta ctaaaaaaac gatgggcgaa atggattgct gctggtagtt attgcttttt
    16261 agtcctatgg attgcgacag agggttattt cttccgcatg tcactagaag atttgatacg
    16321 actttggaca agtttggaaa tcctgacaca aacgtatcag ttgggctttt atctaaacat
    16381 tttgttagga attttgttga taataaagta ttttaaggtt aagcaataga aataataatc
    16441 tgcctgatta tagatgataa actttttgtt tttatctgga ctgtgatatt tatagttcta
    16501 aatggtttat tatttataag tgttgaaaga gattgaagaa aatggcttcg tgactcgctt
    16561 tcttcggtct ttttcgtttt attatcaatt tgtaaacgat ttacaatttg agtcaaatta
    16621 tggtaaaata gtgtcatgag taaaatgtat tatgcagaaa atcctgacgc tgctcacgac
    16681 attcatgagt tgagagtaga gttgttggga caaaaaatga cctttttaac ggatgcgggt
    16741 gtttttagca agaaaatagt tgactttgga agtcaactat tgctcaagtg tcttgaggtc
    16801 aaccaaggag aaactgtcct tgatgtaggc tgtggttacg gacctttggg tttgtcctta
    16861 gctaaggctt atggagttca ggctactatg gttgatatca acactcgtgc cttggattta
    16921 gctcggagaa atgctgaaaa aaataatgca aaagcgacga ttttccagtc caacatctat
    16981 gaacaagttg aaggacattt tgatcatgtc atttccaatc ctcctatccg tgcgggcaag
    17041 caagtcgttc atgaaatcat tgagaaaagt aaagatttct tggaaactgg tggggatttg
    17101 acaatcgtca tccagaaaaa acaaggggct ccaagtgcta agtccaagat ggaggatgtg
    17161 tttggtaatt gtgaaatcct taaaaaagac aaaggatact atatccttag aagtgtgaaa
    17221 gaatgagagc agttgattta atccaaaaaa aacgagacgg tcaagaactg acttcaagtg
    17281 aaattgaatg gctgatagaa ggctatgtat caggaactgt tcctgattat cagatgtctg
    17341 cctttgctat ggctgtttat tttaaaggaa tgacgactcg agaaatctct gatttaacga
    17401 tgaatatggt taagactggg caagagtttg acctgtcagc cattgatggt gtgaaagttg
    17461 acaagcattc tactggtggt gtcggcgata aggtaacctt gattttagct cctcttgttg
    17521 ctagcttcgg tgtgcctgta gcaaaaatga gtggtcgtgg tcttggccat acaggcggaa
    17581 caattgataa attggagtcc attaggggct atcaagtaga acgtagtcaa gaggatttta
    17641 ttcgtcaggt tcaggatatt ggagtatctg tcattgggca atcagaccag ctggtcaaag
    17701 cagataagct tctctatgcc cttcgtgatg tgaccgcaac tgtcgacacg attcctttga
    17761 ttgcgagttc ggtgatgagc aagaaaatcg cggcaggggc ggatgctatt ttgctagacg
    17821 tgactgtcgg tgagggtgcc ttcatgaaga cggttgatga ggcgcgtgag ttggctcaaa
    17881 ctatggtaga tcttggtaag gttgttggac ggaagacagt agcagtgatt acggatatga
    17941 gccagcctct gggacgagcg attggaaatc gtctggaaat ccttgaagca ttggagattt
    18001 tgcaaggtca aggacgtcag gatattaccc actttatttg tgaattggct caaattatgc
    18061 ttggtttggc aaatgttaac aagactgttg aagaagttcg ccaacatctt gagaatggtc
    18121 aagcactggc taagtttgag gaaatggtgc aagcccaagg tggagacttg gaagacctct
    18181 atcgtcctgt aaatgtagct catgtggtgg aaatccctgc tcaggaaacg ggtgtcattt
    18241 cagctcttcc agctatggac tttggtcttt atgccatgag gttgggtgct ggtcgtgcag
    18301 ttaagtctga tgccttggac tatgaaacag gaattgtttt tgaaaagaag attggagact
    18361 ccgttcaaaa gggtgaaatt gttgcaaaag tatacacaaa tgaaaaaatt ccacctcaac
    18421 tagttacaga tttccaaaaa tgtgttaaaa taagtgatga agtgaaaaaa atccgtgaaa
    18481 ttgtagaaat catctcctaa atggagggaa aaccgaaatg aaattaaata aatatataga
    18541 tcatacgctt ttaaaacaag atgcaaagaa aaaacaaatt gatagtttgt tgtctgaggc
    18601 tagagagtat gactttgcca gtgtttgcgt taatccgacc tgggttgaac atgctaaaaa
    18661 aggacttgaa ggcacagatg ttaaggtttg cacagtagta ggtttccctt tgggagcaac
    18721 aacttcagcc gtgaaagcat ttgagacaaa agaagctatc caaaatggtg cagatgagat
    18781 tgatatggtg atcaatgttg gagctctcaa atcaggtaat ttagccttgg ttgagtcaga
    18841 tattcgcgca gtagtggaag caagtggtga taagttagtg aaagtcatta ttgaagcttg
    18901 ccttctgaca gaccaagaaa aaattgttgt ttgccaattg gcccaaaaag ctggggctga
    18961 ctttgttaaa acatctactg gtttttcaac tggtggtgct acgatagcag atgttacatt
    19021 aatgcgtgaa acagttggat ctgatatggg agtcaaggct gctggtggag ctcgttctta
    19081 tgcagatgct cttgcctttg tcgaagcagg tgcgacccgt atcggaacgt cagctggggt
    19141 agctatttta aaaggagaat tggcagatgg cgactactga gttgattgaa ctggcaattg
    19201 aaaccagcaa acatgcctat gtcccctatt ctcactttcc tatcggagca gttttagtag
    19261 ccaaagacgg aaatgtttac acgggagtga atatcgaaaa tgctagctat cctttgacca
    19321 attgcggtga gagaacagct atttttaagg ctatatctga aggccaaaga gaattttcag
    19381 aattgattgt ctatggtcaa actgaaaaac cgatttcacc atgtggtgct tgtcgccaag
    19441 taatggtcga attttttgaa caagatttaa aagtgacctt agtcgcaaaa gataaatcga
    19501 cggtcgagat gacggtcggg gagttacttc catattcttt tacagactta aattagtctg
    19561 agtcgctctt tgagtggcaa gggtctttgt gaccaatcaa tccatacttg caacatcgtt
    19621 gcacatctta tttaggaggt tcagtaatga acaagaaaca atggctaggt cttggcctag
    19681 ttgcagtggc agcagttgga cttgctgcat gtggtaaccg ctcttctcgt aacgcagctt
    19741 catcttctga tgtgaagaca aaagcagcaa tcgtcactga tactggtggt gttgatgaca
    19801 aatcattcaa ccaatcagct tgggaaggtt tgcaggcttg gggtaaagaa cacaatcttt
    19861 caaaagataa cggtttcact tacttccaat caacaagtga agctgactac gctaacaact
    19921 tgcaacaagc ggctggaagt tacaacctaa tctttggtgt tggttttgcc cttcacaatg
    19981 cagttgaaga agcagctaaa gaacacactg acttgaacta tgtcttgatt gatgatgtga
    20041 ttaaagacca aaagaatgtt gcgagcgtaa ctttcgctga taatgagtca ggttaccttg
    20101 caggtgtggc tgcagcaaaa acaactaaga caaaacaagt tggttttgta ggtggtatcg
    20161 aatctgaagt tatctctcgt tttgaagcag gattcaaggc tggtgttgcg tcagtagacc
    20221 catctatcaa agtccaagtt gactacgctg gttcatttgg tgatgcggct aaaggtaaaa
    20281 caattgcagc cgcacaatac gcagccggtg cagatattgt ttaccaagta gctggtggta
    20341 caggtgcagg tgtctttgca gaggcaaaat ctctcaacga aagccgtcct gaaaatgaaa
    20401 aagtttgggt tatcggtgtt gaccgtgacc aagaagcaga aggtaaatac acttctaaag
    20461 atggcaaaga atcaaacttt gttcttgtat ctactttgaa acaagttggt acaactgtaa
    20521 aagatatttc taacaaggca gaaaaaggag aattccctgg cggtcaagtg atcgtttact
    20581 cattgaagga taaaggggtt gacttggcag taacaaacct ttcagaagaa ggtaaaaaag
    20641 ctgtcgaaga tgcaaaagct aaaatccttg atggaagcgt aaaagttcct gaaaaataat
    20701 ggatggaagt catttcttag gaagcggcca agggccgctt ttttaagagt tgagacaaag
    20761 tcattttgtc tcagtaaagc ttgctactag acaaactagc aagttttatt gaaataaaat
    20821 aagactctga aaggaagagc acatggcaca cgaaaatgtc attgagatgc gtgatattac
    20881 caaggtgttt ggtggatttg ttgctaacga caaaatcaac ttgcacctac gaaaaggtga
    20941 aattcatgca cttttaggag aaaatggggc tggtaagtcc acgctaatga acatgttagc
    21001 agggcttctt gaaccaacta gtggtgaaat cgcggtcaac ggtcaagttg tcaatctcga
    21061 ctccccatct aaagcagcta gcttgggaat cgggatggtt caccagcact ttatgttggt
    21121 tgaagccttc acagtggctg aaaacatcat tttaggtagt gaattgacta aaaatggtgt
    21181 gctagatatc gctggagcta gcaaagaaat caaggctctt tctgaacgtt atggcttagc
    21241 tgttgaccct tctgccaagg tagcagatat ctcagttgga gcccaacaac gtgtagaaat
    21301 tttaaaaaca ctttatcggg gggctgatat ccttatcttt gacgaaccaa cggctgtttt
    21361 gactccatca gaaattgatg agttgatggc tattatgaaa aatcttgtca aagaaggaaa
    21421 atcaattatc ttgattaccc acaaattgga tgaaattcga gcagtttctg accgtgttac
    21481 agttatccgt cgtgggaaat caattgaaac cgttgaaatt gcaggggcta ccaatgctga
    21541 tttggcggaa atgatggtag gacgttctgt ttcctttaaa acagagaagc aagcctctaa
    21601 accaaaagaa gtggttttgt ctatcaaaga tttggtggtc aatgaaaacc gtggtgttcc
    21661 agctgttaaa aatctgtcct tggatgttcg tgctggagag attgttggta ttgcggggat
    21721 tgatggaaat ggtcagtctg aactgattca agccattaca ggtcttcgta aggttgaatc
    21781 tggtagcatt gagctaaaag gagattcaat tgtaggcttg cacccacgtc agattacaga
    21841 actaagtgtt gggcacgttc cagaagaccg tcaccgtgat ggcttgattt tggaaatgat
    21901 gatatctgaa aatattgccc ttcaaaccta ctataaagaa ccacatagta aaaatggaat
    21961 tttgaattat tcaaatatta cttcttatgc taaaaagctg atggaagagt ttgatgttcg
    22021 cgctgccagt gaattagttc ctgcagctgc actctcagga ggaaatcaac aaaaagcaat
    22081 tattgctcgt gaaattgatc gagatcctga tctccttatc gttagccagc caactcgtgg
    22141 gttggatgtc ggtgccattg agtatatcca caaacgcttg attgaagagc gtgataatgg
    22201 caaggctgtc cttgttgtca gctttgaatt ggatgagatt ttaaacgtct cagaccgtat
    22261 tgccgttatc cacgatggta agattcaagg tattgtatca ccagaaacaa ccaataaaca
    22321 agaacttggt gtcttgatgg ctggtggaaa cttgggaaag gagaagagtg atgtctaaaa
    22381 aattacaaca aatttcggtt cccttgattt ctgtatttct aggaatttta ctcggagcca
    22441 ttgtcatgtg gatcttcggt tatgatgcta tttggggcta cgaagaattg ttctatacag
    22501 cctttggcag tctgcgtggg attggagaaa tcttccgtgc tatgggtcct ctggtcttga
    22561 ttggtcttgg ttttgcggtt gccagtcgag ctggtttctt taacgtcgga cttcctggtc
    22621 aggctttggc aggttggatt ctcagtggtt ggtttgcgct gtcgcatcca gatatgcccc
    22681 gtcccttgat gattctagca accatcgtga ttgccttgat tgctggtggg attgtcggag
    22741 cgattccagg tattcttagg gcctatctag ggacgtcaga ggttattgta accatcatga
    22801 tgaactacat tgtcttgtat gtagggaatg cctttatcca tgctttccct aaagacttca
    22861 tgcaaagtac agattcgacc attcgtgttg gggctaatgc aacctatcag acaccttggt
    22921 tggctgagtt gactggtaac tcacggatga atattggtat tttctttgcc atcattgccg
    22981 ttgcagttat ttggttcatg ctcaagaaaa caactcttgg ttttgaaatc cgtgcagttg
    23041 gtcttaatcc acatgcttca gaatatgctg gtatttctgc caagcggact attatcctat
    23101 ctatgattat ttcaggtgcc ttggcaggtc ttggtggagc tgttgaaggt ttgggaacct
    23161 tccagaacgt ctatgttcaa ggttcgtcat tagctatcgg atttaacgga atggcggtta
    23221 gtttgcttgc ggccaactca ccaattggta tactctttgc agccttccta tttggcgttc
    23281 tccaagttgg ggctcctggt atgaatgcgg cgcaggtacc atctgagctt gtcagcattg
    23341 taacagcgtc tattatcttc tttgtcagtg ttcattacct tatcgaacgc tttgtcaaac
    23401 cgaaaaaaca agttaaagga ggtaagtaaa gatgtctatt ataaccttgc tccccctctt
    23461 ggtgtcttct atgctgattt actcagcacc tctcatcttt acaagtatcg gtggtgtttt
    23521 ctctgaacgt ggtggtgtgg taaacgttgg ccttgaagga attatggtta tgggtgcctt
    23581 ttctggagtt gtctttaacc ttgaatttgc agaacaattt ggagcagcaa ctccatggct
    23641 atccttgctt gtagcaggat tggttggtag tgttttctct atcatccacg cagcagcgac
    23701 ggttcatttc cgtgcagacc atgttgtcag cggtacggta ttgaacttga tggcgcctgc
    23761 cttggctgtt ttcttagtta aagttcttta taacaaagga caaaccgaca acctaagtca
    23821 aacttttgga cgctttgatt tcccagtctt ggcaaatatc ccagtgattg gtgatatctt
    23881 cttcaagtca actagtctac ttggttatct ggcgattgcc ttctcattcc ttgcttggtt
    23941 tattctcttt aagactcaat ttggtcttcg tctccgctct gtcggtgaac atcctcaagc
    24001 agcggatacc ttgggaatca acgtctacaa gatgagatat ttagggatta ttatttcagg
    24061 ttttctaggt ggaattggcg gagcgattta tgcgcaatca atctcagtta acttttcagt
    24121 gacaactatt gttggacctg gatttatcgc ccttgctgcg atgatctttg ggaaatggaa
    24181 tccaatcggt gctatgcttt ctagtctctt ctttggactt tcacaaagtt tggctgttat
    24241 cggttctcaa ttgccgttcc tacaaggagt gccagcggtt tatcttcaaa ttgcacctta
    24301 tgttttgaca attcttgtct tagcagcctt ctttggaaaa gcagtcgcac caaaagcaga
    24361 tggtatcaac tacatcaaat caaaataagc atacaaaaaa acgtcagtcc aatgactgaa
    24421 ctgcccccca aaagttagac agaaaaaatc taacttttgg ggtgttttta ttatgaaact
//
LOCUS       DAVKAO010000024        24314 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKAO010000024 DAVKAO010000000
VERSION     DAVKAO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.024"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(85..720)
                     /locus_tag="VWX16_001014"
     CDS             complement(85..720)
                     /locus_tag="VWX16_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9158387.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(722..1549)
                     /locus_tag="VWX16_001015"
     CDS             complement(722..1549)
                     /locus_tag="VWX16_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9158388.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINTLFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1571..2636)
                     /locus_tag="VWX16_001016"
                     /pseudo
     CDS             complement(1571..2636)
                     /locus_tag="VWX16_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            3149..3760
                     /locus_tag="VWX16_001017"
     CDS             3149..3760
                     /locus_tag="VWX16_001017"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9158389.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3805..4533
                     /gene="rlmB"
                     /locus_tag="VWX16_001018"
     CDS             3805..4533
                     /gene="rlmB"
                     /locus_tag="VWX16_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9158390.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4571..5482)
                     /gene="trxB"
                     /locus_tag="VWX16_001019"
     CDS             complement(4571..5482)
                     /gene="trxB"
                     /locus_tag="VWX16_001019"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9158391.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQVFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5548..5772)
                     /locus_tag="VWX16_001020"
     CDS             complement(5548..5772)
                     /locus_tag="VWX16_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9158392.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5850..6593)
                     /locus_tag="VWX16_001021"
     CDS             complement(5850..6593)
                     /locus_tag="VWX16_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9158393.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMPFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFTSYKRTYI"
     gene            complement(6593..7393)
                     /locus_tag="VWX16_001022"
     CDS             complement(6593..7393)
                     /locus_tag="VWX16_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9158394.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7551..7907)
                     /locus_tag="VWX16_001023"
     CDS             complement(7551..7907)
                     /locus_tag="VWX16_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9158395.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7901..8431)
                     /locus_tag="VWX16_001024"
     CDS             complement(7901..8431)
                     /locus_tag="VWX16_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9158396.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGESQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(8431..8925)
                     /locus_tag="VWX16_001025"
     CDS             complement(8431..8925)
                     /locus_tag="VWX16_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9158397.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            9060..9506
                     /locus_tag="VWX16_001026"
     CDS             9060..9506
                     /locus_tag="VWX16_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9158398.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            9503..10150
                     /locus_tag="VWX16_001027"
     CDS             9503..10150
                     /locus_tag="VWX16_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9158399.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWILMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIVYYM"
     gene            complement(10171..10752)
                     /gene="pdxT"
                     /locus_tag="VWX16_001028"
     CDS             complement(10171..10752)
                     /gene="pdxT"
                     /locus_tag="VWX16_001028"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9158400.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESYLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(10753..11628)
                     /gene="pdxS"
                     /locus_tag="VWX16_001029"
     CDS             complement(10753..11628)
                     /gene="pdxS"
                     /locus_tag="VWX16_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9158401.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(11780..13159)
                     /locus_tag="VWX16_001030"
     CDS             complement(11780..13159)
                     /locus_tag="VWX16_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9158402.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLTIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            13453..14376
                     /locus_tag="VWX16_001031"
     CDS             13453..14376
                     /locus_tag="VWX16_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9158403.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVNGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            14449..15054
                     /locus_tag="VWX16_001032"
     CDS             14449..15054
                     /locus_tag="VWX16_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9158404.1"
                     /translation="MLLLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            15073..16317
                     /locus_tag="VWX16_001033"
     CDS             15073..16317
                     /locus_tag="VWX16_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9158405.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGINAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(16540..16797)
                     /locus_tag="VWX16_001034"
     CDS             complement(16540..16797)
                     /locus_tag="VWX16_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9158406.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(16839..18875)
                     /locus_tag="VWX16_001035"
     CDS             complement(16839..18875)
                     /locus_tag="VWX16_001035"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9158407.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKKIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(19135..20052)
                     /gene="glyQ"
                     /locus_tag="VWX16_001036"
     CDS             complement(19135..20052)
                     /gene="glyQ"
                     /locus_tag="VWX16_001036"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9158408.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISNQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(20247..21008)
                     /locus_tag="VWX16_001037"
     CDS             complement(20247..21008)
                     /locus_tag="VWX16_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9158409.1"
                     /translation="MENLYLVKDDSPLAAFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSNFNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWLSFLIVDKLVENLG
                     SVQAVFDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(21281..22123)
                     /locus_tag="VWX16_001038"
     CDS             complement(21281..22123)
                     /locus_tag="VWX16_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9158410.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(22237..23628)
                     /locus_tag="VWX16_001039"
     CDS             complement(22237..23628)
                     /locus_tag="VWX16_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9158411.1"
                     /translation="MDKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKKHFRKGQAEVIAYYPIQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(23863..24243)
                     /locus_tag="VWX16_001040"
     CDS             complement(23863..24243)
                     /locus_tag="VWX16_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9158412.1"
                     /translation="MTCQFIRDMLDLPAKNVTILEGSDIHVLPSMPYSAQDFYTSIDD
                     VLAELDNGTQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKHVAP
                     VYAIAIVDSNYFSDDLAFHSFIVK"
BASE COUNT         7100 a         5490 c         4339 g         7385 t
ORIGIN      
        1 taaattcctc cgtcactttt tattttaaag gattttagaa ttttttacaa gatattatgc
       61 tcaaatttaa cattatttag ctatttagta aagatagtct ttcaaggctt ttgacaaagc
      121 ctgataacca gcaatactga ggtgcagtcc atcagttgta tattcttttt tgagttggcc
      181 tgcttggtct gtcaaacaat caaatactgg cacaaattcc acctgcatat aggcagatgc
      241 aagctcttga taggcttgat tccagttctg aattttttca ttcgagcgga tatagactgc
      301 ctgctggtac tcctctccct cattgacagg caaaatggaa agcaatttaa tctctgtcaa
      361 tggataatcg cgagcaacgg attgaatgat agcttcgaga ttattgagag cctcattcac
      421 aggaacatcc tttccgatat catttgtccc aatcagaaga aaaattttat ctactgctcc
      481 accatataga tgagcatcaa ggttctctag taacagtcct gtctgatagc cacgaattcc
      541 tcgattgaca atcgtctttg aagtcccaaa tagctcctgt agaggataat actcgacaat
      601 ggaatcccca ataaaaagaa tgtttggttc tacaacagaa atgtgattta ggtgacgata
      661 cttggtttga attttttctt gttcctttag gagccaattt tctaataact gtactgccac
      721 ttcattctcc tttttctaac cagttttcca agacttgata aactcctcct tggctgttgg
      781 ctggtgctag agcagtcgcc acagctttgg ctttctcatc agcattttcc atcgcatagg
      841 caattccagc catttcaagc atttcaacat cattttcact atcaccaaaa gccatgattt
      901 cttgggattt caagtcccag cgcttgagta attcctccaa gccccatgct ttatgaatcc
      961 cagcttggag gatatcaatg caaccatagc cactggatac agctcggaca cggccatcaa
     1021 agagagtatt gatttcttcc aaaaccgaac tcaaacgttc ctcaccaaca accatgctca
     1081 tcttgagcac accaccaaag aggtcagatg ttaattcatc cacaaattgc atccgttggt
     1141 agaatttttc aatcatttct ggagtcataa aactttcaag atctgtaaaa atcgtacctt
     1201 ccttgacaaa atcacccttc atccccgtta caacaaactg gtcctgacac gctcgaccct
     1261 tgaaatgagc caaagccttg ttgacaatgg cgtcatccca tgtctgagcc tgaatcaatt
     1321 cattgttttc aaaaatacga gcgccattag caacaaccag aaccactcga tccaccaagg
     1381 gactcagtag ttgtctcatg cggtgaattt cattgcccgt cgcaatgaca aaacgaatgc
     1441 ccctttgatc caactgatct aaaatctttt ccaatcgtgg gagatcaagc tgacctctag
     1501 catccagcaa ggtcccatcc atatctgtcg caattacctt aatcatcatt tactttcctc
     1561 tggattcaaa ctaataccac ttcaatccca catgttcatt catttcttta taggctgtat
     1621 caattcgttc cttagtcgct tcatctaact ggtcacgatg actgccatcc tctataaaat
     1681 cttccaaaat ataagtttgg taatgataca agggatatcc ttctggtgaa aaggttccct
     1741 ttggtgttcg attgacccaa gtaggatgat ctttagctgt tccgatagtt gtttttccat
     1801 cattcttctt gatggtgaca tccatgagaa caccacgttc agtccactta gcattctctt
     1861 catctcccat agattcaatt cgttgattgg aaatgaagtt ccccattgaa tagataatga
     1921 gtttcttatc tccatctttt tcaaccgttt cagatggctc aacaacgtga ggatgccctc
     1981 caaagataat atccgctccc caatcgatca tcttgtgata aagagctttt tgttcttcag
     2041 ttggttccaa tcgatactca acacccatct gaggcatgat aatggtgata tctgcttcct
     2101 tctctgcccg ttcaacttca gccttcatct tatcttcgtt taaatctaaa agataacgat
     2161 tatagtcttc ctgagaaata tactgctcaa ttccattgaa accataggaa taggctgaca
     2221 atgcaacctt gataccattc acttccttaa tgaccagcgg aacctgatca cgtggttcgt
     2281 gcgtataaac tccgattgga gtgattccag ctttctcaat aatatcggcc gttgaaataa
     2341 ctccctcaat ttgcgaatcc aaaatatgat tatgagctaa atccagcaca tgataacctg
     2401 cctccttaat agcatccata acttcagcag gagcattaaa gagaggataa cccgctaaat
     2461 aatgatcctt attaatggtt tcttcaaaat caccaatagc taaatctgct tgcttagtcc
     2521 acttgcttga gccaaggagt cacatactca aaattttcat gaaagtcata ggtaccgtct
     2581 tcttttttag ctgaaaagaa aagtccatcg tggtagagca aatccccgtg ggccataatt
     2641 ctggcagttt tttcctcttc ctactcctga gacttttgct tagtcccctc ttgagaaata
     2701 gtatctcgtt tttgactagt caagggattc cttggaagct ttcaaacaac aagaccaagc
     2761 ccattgataa accaactgct agcaagagta tcgccacaaa tcccttattg ctccacttgc
     2821 gataactcct aaaaagttta ccaagccctt cataaaacga aaagctaaac caccctgatt
     2881 tcgattttgt cttctttgta tcttcgttct ccctactttc ttatgcaagc cttttctttt
     2941 tattatatca cagataagta tttctttcac aattgaattg aacttcccat ctattttcta
     3001 taaatcctaa atgccataat gctttcaatt cttgtcattt tgtgatatca tgtagaggaa
     3061 atgaactaat ccacagtggc ttattccaag tataccactt gggctttggc agtagctaac
     3121 tgcactaaac ataatataag gagagaaaat gtctgcaata gaacgtatta caaaagctgc
     3181 tcacttaatt gatatgaacg atattatccg tgaagggaat cctactctac gcacggttgc
     3241 tgaggaagtc actttccccc tatctgacca ggaaatcatc ctaggcgaaa agatgatgca
     3301 attccttaaa cattcccaag atcctgtcat ggctgaaaaa atgggactcc gcggtggtgt
     3361 tggactggct gctccccagt tagatatctc aaaacgcatt atcgctgttt tggtacctaa
     3421 tattgttgaa gaaggcgaaa ctccacagga agcctacgat ttggaagcca ttatgtacaa
     3481 tccaaaaatc gtctctcact ctgttcaaga tgctgctctt ggcgaaggag aaggttgcct
     3541 gtctgttgac cgtaacgtgc ctggctatgt tgttcgccat gcccgcgtta ctgttgacta
     3601 ctttgacaaa gatggagaaa aacaccgtat caaactcaaa ggctacaact ccattgttgt
     3661 tcagcatgaa attgaccaca ttaacggtat catgttttac gatcgcatca atgaaaaaga
     3721 cccatttgca gttaaagatg gtttactgat tcttgaataa agaaaatccc gttgcaagac
     3781 ggggttttgt gttataatag aggcatgaaa acaaatgata ttgtctatgg tgtccacgcc
     3841 gttaccgaag ccctccttgc aaatacagga aacaaactct acctccaaga agatctccga
     3901 ggtaagaatg ttgagaaagt caaggaacta gctacagaaa agaaggtgtc catttcttgg
     3961 acatcaaaaa aatctctctc tgagattact gaaggtgctg ttcatcaagg ttttgttcta
     4021 cgagtgtctg aatttgccta tagcgagcta gattacatcc ttgcaaaaac acgccaagaa
     4081 gaaaatccac ttctattgat tctagatggt ctaaccgatc cccataatct gggttctatc
     4141 ttgcgaacag ccgatgcgac caatgtttca ggtgtcatca ttcccaagca ccgtgctgtc
     4201 ggagtaactc ctgtcgttgc caaaacagcc acaggtgcta ttgaacacgt tccaattgcc
     4261 cgagtgacca acctcagtca aaccttagat aaactcaagg atgaaggttt ctggaccttt
     4321 ggaacggata tgaacggtac tccttgccac aagtggaata caaaagggaa aatcgccctc
     4381 atcattggaa atgaaggaaa aggtatctct agcaacatca aaaaacaagt cgatgagatg
     4441 attaccattc cgatgaatgg acatgttcaa agccttaatg ccagtgttgc tgcggccatt
     4501 ctcatgtacg aagttttccg aaatagacta taaaaagttt ccagtcatct gattggaaac
     4561 ttttttataa ttaactatgt tctgtaatga atttataggc ttcttgacca gcgatagctc
     4621 catctccaac cgctgttgtt acttggcgaa ggtctttcaa gcgaacatct ccaactgcaa
     4681 agataccgtc gactgcagtt ttcatgtggt tatctgtcac aatccatcct gcctgatctt
     4741 ggatattcaa ttctttaaca aaatcgctaa gagggtccaa tccaacatag ataaagacac
     4801 caccgaagac ttgttctgtc acttgacctg ttttcacatt ttcaaatacg actgattcta
     4861 ctcggttttc acccttgatt tcccttacta cagaatccca gataaagctg attttttcat
     4921 tcgcaaaggc gcgatcttgt aaaacctttt gggcacgaag ttggtcacga cggtgaacaa
     4981 tggtaacagt cttagcaaaa cgagtcaaga agagggcttc ttcaacagct gaatctccac
     5041 caccaactac caataaatct tggtcacgga agaaagcacc atcacacaca gcacagtaag
     5101 aaacaccacg actgttcagt tcttcttctc caggtactcc caaaggacgg tgtttagaac
     5161 cagttgctac gataactgta cgtgtttcat atgtttggtc atcagtcatc actttcttaa
     5221 aatcaccatg gtcttcgaca ttttcaacat aaccataaat gtgctcaaca ccaagatttt
     5281 caagtggttc aaacatcttt tcagccaatt caggtccact aatattagcg tatcctgggt
     5341 aattttcgat atcagatgta ttattcatct gaccacctgg cagaccacct tcaatcaaag
     5401 ctacttttag attgcttcga gcagcataca aggccgcagt catccctgca ggtccagcac
     5461 cgataataat agtatcgtac atatagattc cttctttctt gttgtaacta tctttattct
     5521 aactctttct tgtcaatcac gcaaggatta tgccttgaaa acaagaatta aactcataac
     5581 cgcaaaggat aatactggga caaccaagac tccaatcata atcatatcat agagatgggc
     5641 ttggcgagcc ttggctgtct tatcaactcc cgacatggct ctcagaccaa tccaaatccc
     5701 taaaaaaatc agaacaagga tggtggtcaa gatcaaactc tcgaaatata aagaaaatag
     5761 ttgcagtagc atgatttctc tcatttctat cttttttaaa gagtaaactc agctagtcca
     5821 actaactgag ttttccttta tctattatat caaatataag tccgtttgta actagtgaag
     5881 aattcttttg tccgctcttc tttaggggtg tggataatct catccggagt tccagactcg
     5941 atgattttcc ccttatctaa gaagagaatt ttatccgcaa cttgggctac aaagggcatg
     6001 tcatgactga ccaaaatcat ggtctgacct gacttagcag catctgcaat agacttttct
     6061 acttcaccga ccaattctgg gtcaagggct gaagttggtt cgtctaagag caaaacatct
     6121 ggtttcatag caagcgcacg cgctagggca acccgttgct tctgtccacc tgataaatgg
     6181 cgaggataat ggttttcacg gtccgaaagc ccaaccttag ccaactcttc cttggcaatc
     6241 ttagtcgctt cttggtcaga taatttcttg acaacaacca agccttcttt cacattatca
     6301 agtgctgttc ggcgttcaaa taaattaaac tgttggaaaa ccatagacaa cttacggcgt
     6361 agggcaagga tttcttcttg agtgatttta gaaaaatcaa ctgaaaaacc atcaatctga
     6421 atagagccac tgtcaggtgt ttcaagataa ttgagactgc gaagaaaggt tgattttcca
     6481 gctcctgaag aaccaatcaa ggctacaact tccccttttt gaatatccaa gttcagatga
     6541 tccaagacag tctgtcctga aaaggatttg cttaaattcg aaatcttaat cattaacgaa
     6601 ggtctccttt cacatctgtt tgcactgtat caggtgcaga aatagccatt tttctctcga
     6661 tgaaacgacc gaggctttca attccgatat tgactaccca ataaacaagg gcaacagaga
     6721 tgaagcgttc aaaatagcga taatcagctc cacctagaat ctgagcttgg gcaaagactt
     6781 ccacaacacc cgcactaaaa gctagagatg ttcccttggt caaaccgatg agggaattaa
     6841 tcaaggttgg agtagctacc accgctgcat taggaataat cactcgtcga taaacttgcg
     6901 ctcgggtcat acccagactg cgcgccgcct caatctcacc aggattaact gagagaatgg
     6961 ctgcacgaat ggtttcacta gcataagctg cctcattaaa ggcaaaagcg acaatcgcaa
     7021 aagctgcagc tggaatcgca ttgatattga gaccagttcc ccattgctga ttgagggctt
     7081 tcaaagccaa agggattccg tagtaggtca acatgagttg caccaaaatc ggtgtccctt
     7141 ttaagaaact aacaaagaag gcctgcaagg gatataaaat cttgacacga ttgatcttca
     7201 caatggcaaa aagaagcgcc aaaaccaagc caaaaagggc accgccaatt gtcaacataa
     7261 ttgttgttgg aagttgttgg acaattctag ggattccatc aaagaccgaa cgtaggctaa
     7321 acagcttgcc atccggaatc aattgcatca agttttggta ccaatctgat gctaaaatcg
     7381 ttgtaacatt cataaaaaca tcctcttctg ataatgattc attctaccat gcttctgccg
     7441 tcaatgaaat tatttgctac ggtcagatac agactttgtc tacctactag tttatcatac
     7501 tttgaaacag ggaagttata tattttatct atcaaagaga tagataaaaa ctatttcagt
     7561 cccagttctt cataagattt tcgataaccg atttgcttaa cagttccatt ttctactaaa
     7621 atgggccgtt ttaacaacat accgtcactt gctagcaact cagccgcttc ttggtttgac
     7681 aaacttccta ccttatcttt tagccctaat tcacggtatt tgataccact ggtgttgaaa
     7741 aattgcttca attcaaatcc tgaggtttct agccaattca aaatgacttc ttggctaggt
     7801 gtttcttcca cgatatggac ggctttatag tccacaccta attgatttaa ttcttgtttt
     7861 gcttttttac aagttgaaca tttggggtat tcgataaatt ctaacatgtg cttctccttc
     7921 tattgtttct atctttaaaa tctactcctt catgctccaa gagccaagct ttcttttcca
     7981 ctcctgcagc ataacctgtc agacgcttgc ctgctcccaa cacacgatga caaggtacta
     8041 ggatagacca aggattgcgt cccactgctc caccaattgc ttgagcagaa gccacttgca
     8101 ggtcttgagc aatttgtcca taggtcactg tctgaccata aggaattccc tgtaaatagg
     8161 accaaactct cttttcaaaa ttcgttccaa ttggcgccaa gagcaagttg gataaatcct
     8221 gagactcgcc tttaaagtaa tcatctaagc aagcaataac tgggtctaaa ataggatgac
     8281 taacaacttc ttctatcgtt tcatctccta gtcccctctc aaaatgcttc tgctcctgaa
     8341 cccaaattcc atacaaataa tggtcatcag ctacaagtga tagaatacca attggtgagg
     8401 agtagagtat ttttacatac ttcttttgca ttatttcttt aatttttgat aggccaagcg
     8461 ttcgccatct actggcacct taccaacttg tttaaaacca agtttttcaa aaatatgttg
     8521 cataaccttg ttttcagcat gcgtatctga gcgaaaatca agataatcaa aaccttcaat
     8581 caagccctct aagaaggttt gagcaactcc ttttccctgc acatctgctg ccacagcaat
     8641 acggtgaaag actagatact ctgactctcc agcttgccag tttccctcat aaatagcttc
     8701 ataggcctcc tctggactct tggtcacagc agcataggct agcagttctc cctcttccaa
     8761 ggctacgtag gcttgccctg agatgatatc atcgataata acatctgcat ttggatagcc
     8821 attttgccac tggtcactac cagcatctgc taaacatttt ttggcatcct ccatcacctg
     8881 catgatggca tctacttcat ttggaaaagc taaacgaatt tccatctttt ttcctcattt
     8941 ctgactagtt tctcccatca tagcataatt taagtatttc tacaagcagt taaaacttga
     9001 ctttgtgttt ttgttatttt ataatctagt tatcaaaact atataaaaag gagttgaaaa
     9061 tgaaaactac cttttcctac ccaaaatggg cagaaattcc aaacattgac ctctatctgg
     9121 accaggtttt gctctatgtc aatcaggtct gcgcccctat ctctcctaat aaagacaagg
     9181 gcctaacagc atctatggtc aacaattatg tgaaaaatgg ttacctgaca aagcctgaca
     9241 aaaaaaaata ccaacgccaa cagattgccc gtttgattgc tatcacaacc ctcaagtctg
     9301 tattttctat tcaagaaata gcccagacac ttaatactct acaaactcaa gcaagttcag
     9361 accaactcta cgatgctttt gtggactata tgaaccaagg aattgaccca gctaacccta
     9421 ttatccaaac cagctgccaa accgttaaac tctatcatca aactctagac ttaatcgatc
     9481 atactcaaga ggaggtaatc caatgaacac tagtcttaaa ctcagcaaac aactcagttt
     9541 tggagaggag attgctaata gcgtgaccca tgctgtgggt gcagtcatca tgcttatctt
     9601 gctgcctatt tcatccatct atagttatga agcacacgga tttttatcct ctatcggcgt
     9661 ttccattttc gtcatcagtc tctttctcat gttcctatca tccaccattt atcactctat
     9721 ggcctatggt tcgacccaca aatatgtttt gcgaatcatt gaccattcta tgatttacgt
     9781 tgccattgcc ggctcataca cgcccgttgt cttgaccttg atgaataact ggtttggcta
     9841 tctgattatt gtcatccaat ggggaacgac catctttggt attctctata aaatctttgc
     9901 taaaaaggtc aatgagaaat ttagccttgc tctttacctg attatgggct ggttggttct
     9961 ggctatcatt cctgccatta tcagtcaaac gacacccgtt ttctggattc tcatggtaac
    10021 tggcggactc tgttatacag ttggagctgg attttatgcc aagaaaaaac cttatttcca
    10081 catgatttgg catctcttta tcctagctgc gtccgtactc caatacatcg ctattgttta
    10141 ttacatgtaa aaaagttgag aaattcaatc tcaacttttt tctttacaca tattgataaa
    10201 gtactggtgc aagcgcacat catcagtcaa ttctggatga aaagaactta ccaacatatt
    10261 tttttcttgg gctgcaacaa tttgattgtt cactgttgct agaatttcta caccctcacc
    10321 aacactactg ataatcggac cacggataaa ggtcattgga atcttgccaa ctcccttaca
    10381 ttctgcttcc gtgtagaaac ttcctaattg gcgcccataa gcattacgct cgaccaccat
    10441 atccatagtt cctagataac tctctttctg agaagtgatt tccttagcca gcaaaattaa
    10501 gcccgcacag gtcccaaaca ctggtaagcc agatagaatg gcttctcgga tgggaagtag
    10561 catgttctgg tcacgtaaga gcttgcccat ggttgtagac tcaccaccag gcaaaatcaa
    10621 acccgacaag tcactctgat cttgctgaaa atcatctaga tttctgagtt ctacactctc
    10681 gacacctaat tgatctagca cttttgcatg ttctgcaaag gccccttgca aggccaatat
    10741 tccgattttc atctattttc ctcgttcagc catgagaatt tggatttcat tttcattaat
    10801 accaaccatg gcttctccta aatcttcaga gatttgagct aggatttgag gattacggaa
    10861 gttagtcaca gccttaacaa tggcactcgc tcgtttaaca ggatctcctg acttgaaaat
    10921 acctgaaccg acaaagaccc cctctgcccc taattgcatc attaacgcag catctgctgg
    10981 cgttgcaaca cctccagcag cgaaatttac aactggcaat tttccatgtt catgaacata
    11041 ttggaccaat tctacaggga cttgcaaatc cttggcagca acataaagct cgtcctcacg
    11101 taagttttga atgcggcgaa tttcctgatt catcatacgc atatgacgaa cagcttggac
    11161 gatatcccct gtccctggtt ctcctttggt acgaatcatg gaagcacctt cagcgatacg
    11221 acgcaaggct tcacccaaat ccttagcacc acagacaaaa ggaacttgga attctttctt
    11281 gtccacatgg aaacggtcat cagctggaga tagaacttca ctctcgtcga tataatcaat
    11341 ttcaatagcc tctaaaatct gagcttcaac aaaatgcccg attctgacct tagccattac
    11401 tggaatacta accgcttctt ggatttcctt aatcatcttt gggtcgctca tgcgggaaac
    11461 tcctccagct gcacgaatat cagccggaat tcgttccaag gccatcacag ctgccgcacc
    11521 agcagcttct gcgatacgag cctgttcagg attctgcaca tccataataa caccaccctt
    11581 gagcatctgt gccaagtttt tatttagttc ataacgattt tcagtcattt gttttatcct
    11641 catcatttgt attggtagct accttttcat tttaagtcag aacagaatga agcacaagat
    11701 aaaaaaacta taattgtatg gggacagatt ggctaaaaac tatctgacct taacttaagg
    11761 ccagatagct cattcatttt tatttttcag ccgtaagggc agccattgtg atgtagttgt
    11821 atggtttatt gaagtgtggc aagaagaaga ggtctgtcaa tgccaattta tcaattgtca
    11881 catgctcttg gatagtaagt gagaacatgt ggattcccat gctaattgca atatcatgtg
    11941 aaaccatttg ggcaccaaga atttcacggc tatctttgtc aaagacaatc ttaattgcta
    12001 cttcatggtt gtcatgtttc atgaattctg gtttttgaag atcgttaaag cctgtttcag
    12061 ttgcgttgta accagcagct ttcgcttttt caagagtcaa accagttgaa accatgtgaa
    12121 gaccgtagat tgagatacca tttgaacctt gaacaccgat tccttccaat tcatgtccac
    12181 aagcgttgta ggcaccaacg ataccagtgc gaacagcatt tgaagcaaga gcgatatagc
    12241 ttgtatcttt acgagcattg tcataaacag tcgcacagtc accaacagcg taaacgcctg
    12301 ggatagatgt ttcttgtttc ttgtctacaa ggaaggcacc gttgcggaag agttcgatct
    12361 taccatcagc aagggctgtg tttggacgga aaccaactgc aaggataacc atatccacgt
    12421 caaagctttc tttgtcagta atcaagcgtt caactttacc atcaccttcg attgctttaa
    12481 cagtttgacc tagagccaag cggatgttgt gatcttccaa gttcttcgcc atcatttgtg
    12541 tgaagtcttt gtcatagtaa ccgttcaaga cagtatcaac gatatcaaca aggacaactt
    12601 cttttccaag acgttcaaag gcttcagcaa gttcaacacc gatgtaacca ccaccaacaa
    12661 cggcgatacg gtcgaggtgt tggctcttgt cagaaagttt attgataact tcttcagcat
    12721 tttggtacaa tttcacgaat tgtacgtttt caagagttgc tttaaattcg cggtttcctt
    12781 taacaatttc aacaccttcg attggtggca agattggtgt agaacctgta gcgaaaatca
    12841 atttttcgta tgattctttg tgctcttttc cttcaacttc cgctgtaact actttgttat
    12901 catagtcgat tgaaagaaca ggtgagttca tgtaaacttt agcaccttta gcttccaatt
    12961 tttctttatc agaatagaac aagccttcag caccgtcaat ttgttcacca atccaaagag
    13021 ccattccaca tcctaggaaa gagatgttag agttttggtc aaatacaaca atttcgttct
    13081 catttccaaa attatccaac atggtattga tacatgctgt accagcgtgg ttagcaccga
    13141 ctacaacgat tttactcata aaaatttcct gccttcattc taaatttacc ttactagtat
    13201 accatttacc gttagcgttt acaagggggg tactattatt tcagcatttt ttatcaaaaa
    13261 taattcccca tttatcctgt tttcaagcct catgaatttt atgagcatat ttcttgactt
    13321 tttccaaatt ttgtttgtga aaattctaac tttatggtat tctaataaca tgaaaataaa
    13381 atgtaagggc ttcaatttag ataattattt tatattacaa cttattttct ataagagaaa
    13441 ggagttggta gcttgactct tcgttcacat tctgaacggc taatggggac tactatcact
    13501 atttcattag tggatgagca agccgatatc tttctccaaa aatcctttga cttgctcaaa
    13561 gagcttgaat accgcttcaa cgccaatagt caagaatctg agttgatgga aatcaattat
    13621 caagcaggaa tatcccccgt cacggttcat ccagacctgt ttgagctgat ttcacttggg
    13681 ttagagcata gcctagcgct ctctagccac ctcaatatca gcattggtcc cttaattcaa
    13741 acctggcgta tcggtttttc agatgccaag gtcgcccagc ctcaagaaat tgaatcggtg
    13801 ctgcctctaa tcaatcctca tggtatcgag ttagattctt ctacttctac tgtgttttta
    13861 aaacagaaag gaatgaagat cgatctaggt tgtttagcca aaggatacag tgcggataag
    13921 gttgcccaat ttcttaggaa agagggggtg acttctgcct tgatcaatct gggagggaat
    13981 atcctgacca ttggaaaaaa tcaggcaaga ggggataacc catggcaaat cgggattcaa
    14041 gacccagcca atcctagggg aaatcattta atgaccatcc ctgttgtcaa taaatctgtc
    14101 gtgacttcag gcatttatga acgtcacctg accgtcaatg gacaagatta ccatcacatt
    14161 tttgacagtc aaacaggata tcctgttgaa acggaactag cgagtctaac aatcatctct
    14221 gataaatcag tcgatggcga aatctggacg actcgactct ttggagaaag acctgcttct
    14281 atcctctggc aagtcgaaag tttggagggc atcgaagtca tcctcatcga taaagaaggc
    14341 cacctaagct gttcttcagg aattcctact ctatagagac aactgtttca atggaatttt
    14401 aaaattgtat tatgtaaaaa gagaaaggaa atctcatgtt aaaacttatt gctattgctt
    14461 attgctattg ttggaacaaa ttcaaaacgt tctacaaacc gtcaattgct tcaatacatg
    14521 caaaaacact ttgctgacaa agctgaaatt gaacttgttg aaatcaaggc cattcctgtc
    14581 ttcaacaaac cagctgacaa gcaagtacct gctgaaatat tggaaattgc tgctaaaatc
    14641 gaagaggcag atggcgttat tatcggtact cctgagtatg atcactctat tccagctgtt
    14701 ttgatgagcg ctcttgcttg gttgtcttat ggtatttacc cacttttgaa caaaccaatc
    14761 atgattacag gtgcttctta cggtacgctt ggttcatctc gtgcccaatt gcaacttcgt
    14821 caaatcttga atgctcctga aatcaaggca aatgttcttc cagatgaatt cttgctctca
    14881 cactctcttc aagcatttaa cccaagtggc gacttggttg accttgatgt tatcaagaaa
    14941 ttggatgcca tctttgatga cttccgtatc tttgtaaaaa tcacagaaaa attacgtaat
    15001 gcacaagaat tacttcgcaa agatgctgaa gactttgact gggaaaattt gtaagatagg
    15061 agaccaaaaa gaatgaaatt tgttggactt gttggatcaa actacgatca atcatataac
    15121 cgtaagctct tggaatttat ccgccgctac ttcaaactca aatttgaatt agaagttctt
    15181 gaaattgacg aagttccaat gtttaaccaa gatgaaaaat gggatgaaag tttccaatta
    15241 cgttatttat ataacaaaat tactcgtgca gatggtgtta tcattgctac tcctgagcac
    15301 aaccacacta tctcagcttc actcaaatct gtacttgaat ggctttcata cgaagttcat
    15361 ccatttgaaa acaagcctgt tatgattgtg ggtgcatcat actatgacca aggaacatca
    15421 cgtgctcaag ttcaccttcg taaaatcctt gatgctccag gtattaatgc ctacacgctt
    15481 ccaggtaacg aattccttct tggtaaagct aaggaagctt ttgataacaa cggaaatatc
    15541 accaacgaag gcactgttaa attccttgaa acttgcttag ataactttgt taaatacgta
    15601 ggagtcgttt cgaaattgaa aaaaccaaaa ccaatcgaat cagaagactt ggattgtgga
    15661 aaaccaatcg ctacaaccat cacagaagtt gaccctgacg atccagaatg ggtagaaaaa
    15721 gttgcagcaa tcactggtgc tgtttctggt gatacctatg tcaaattgga ccacggtatc
    15781 ctaacagtta accaaattga tatgttcttg aaagctatgc catttgaatt gacatacgct
    15841 gatgacaaca accaattcct ctactacaac aacgctcacc aagatccaga cactatgttc
    15901 gctaaacgtg taccacctca atcaggtagc cgtatgtcga ctgttcatgg ttctcttcca
    15961 ccagcacgta tgaagaacgt agagtgggtt atcggaacac ttcgcaacgg aaaccaagaa
    16021 tacgtccgta cgatcgttcc aggttctcct gcaggtgtta tcaacactca caactaccaa
    16081 gcaatgtact atcctgatgg atcatacgct ggtatcaatg aaatcgtctt taacttccaa
    16141 ccatggcttg actggtacct aaaagagact ggtcaacgtt tggtgggtgg tagcggacca
    16201 tttgctcctg ctgccggagg tcatggagac gccgatgcta cttctggcgc ttctgattca
    16261 ggggatgctg gaggccacgg tggtgacgca gacgctacag ccggtgcaag ttactaatga
    16321 tagtttttag gaaccattat ggttcctttt ttagcgaaaa aagactagca aacacaaact
    16381 gctagtctct ttgttatcgt ttcatctatc cttgttaaaa gaaagaatac ttatactcaa
    16441 tgaaaatcaa agagcaaact aggaagctag ccgcaggctg tacttaagta cggcaaggtg
    16501 aagctgacgt ggtttgaatt tgattttcga agagtattat tatgaatttg ggtcatcaag
    16561 actacggcca tgtaatcctt tttcacgttg tacttggcgt agtttttctg gtgtaacatc
    16621 gtttccttct tcgtccacaa ttttgattcc ttcaatgtgg tgacgaacag cgcggcgata
    16681 accttcgatg tactcctcac gtagtttggc ttgttccact ttttcttctg gtgttaaacc
    16741 ttctgttttt ttctttttag caagctcatt gatacgagca atttttttcg gatccatttc
    16801 ttctcctttg taataagata aagtccgttt atcaaatttt atttagtgtt aatttggtta
    16861 aaacaagcaa acttagctgc tttcttggtt agttgtgaca agattgccaa acgattttga
    16921 cggacagcct gatcttcagc cattaccata gtattttcaa agaaagcatc aatgactggg
    16981 ctaagcgcaa agagttgttt caattgctga cttgcaggtc ctgataaaac gagtgtttct
    17041 actgcttctg ccaaagcttt ttcttggtca ttctcaaata gtgctgaatc aactgtagca
    17101 accccttctg ccttctcagc caggttaaag gcacgagaaa gtgattcaac agatggttta
    17161 aaatcttctt ccttgcttac ttctacgaga gcacttgctg cttccaacat atctgccaca
    17221 acaaagtttg aacctgcaag aactgcttcc ttgatatctt ttggagtaga gcccatcatc
    17281 ttatcaacac gagccttgat aaagtccata acctctgctt tattttcata agtcaaactg
    17341 tcaaatttca atgcataaag gctatcaatc agctcatcca tagcaatgtg ccaaccaaag
    17401 gcatccaaga tacgaaccac accttgagtt gcacgacgaa gggcataagg gtcattagaa
    17461 cctgatggaa tcaatcctac tgagaagaaa ctcaaaatcg tatccaattt gtctgcaatg
    17521 gctagaactg caccgacctt gctctctgga agttctcctt cagctgatgt aggcatgtag
    17581 tgttcacgaa tagcagctgc caccgctgga gtttcaccag caagaagggt gtatttttca
    17641 cccataattc cttggagttc gtcaaattca ccaaccatac ctgtcaacaa gtcaaacttg
    17701 taaatggctg ctgcacgagc aaggtcaact gtttcatcca ctgacaaacc agctttttct
    17761 gccaaaagta cagtgatttg acccgtacga atcatgtgtt cacgaaggga accaatcttc
    17821 tcatggaagg tgacattgtt taatttttca acaagatctg aaatcaccaa tttttggtct
    17881 tcacgccaga agaattctcc gtcttccaag cgggctacca agactttttc atttcctttg
    17941 atgacatttt tcaaacgctc tgcgtttccg ttacgaacag aaatgaagtt tggcaagagt
    18001 tttccatctt gatcacgaac aacaaagtaa cgctggtgtt ccttcataga agttaccaag
    18061 acttcttctg gaacttcaag gtatttagca tcaaaacttc ccatgaaggc agttgggtat
    18121 tcaaccaaat tcaagacttc attcagcaaa tccgcatcaa tttcgatacg tacaccatgt
    18181 tttgcctcaa ttttcttgat ttggtcaaca atcatttgct cacgttcaca tggatctgcg
    18241 attacaaact gcttacgaag gtcttcttca tagctcaatg ctgactgaat tttggtttct
    18301 ttccccaaga aacgatggcc acggctcaca cgacctccct tgatatcaag gaaatccaag
    18361 tcaaactctt gctcatccaa gagaacagtt aaagtgtgaa cagggcggat gtattcaaag
    18421 ctgtttccag cccagtgcat gctgacaggg aaagtcaatg acttcaagac atctacaaca
    18481 ccaggaacaa tggcttcaac tgcttgacca acttcttcct tagtgacata gacatattct
    18541 tcacccttga tttcacggaa ttcgatatct tcaacagtca agccttttcc acggacaaat
    18601 ccttgagctg ctttggtgaa gtttccatca ctatccaagg caattttctt tgctggtccc
    18661 ttgaaatctt ctgtcaaatc agactgtttg tctgataaac cagtcacacg aacagccaaa
    18721 cgacgtggtg ttgagaaggt ttgaatggct tcaaaagaca ggcggttttc cttgaggaag
    18781 gctgccattt tttcgcctag ttgtttttca cttggtgtga caacataggc tggtaattct
    18841 tcaagaccga gttctactaa taagtttttt gtcatgtttt ttcctttaca ttttcttcaa
    18901 tcttttttga tatgcacctt aggtactggg gacgtcgcta actaactctg tgagtctgta
    18961 tggaaatcca atgctaaatt tatcaagatt tccaacaatc tcatcaaagt ggatttagct
    19021 gactgatttt tgaacctaag gtttcaaaaa tccccacata acagtacggc ggtgcatatc
    19081 cctctagcaa gtcttcgact tgcctctaac gattttagag tacagtatta ctcactattc
    19141 tgcgtcttct gctaaaagtt taactcgtgt ttcttcatcc aaaagtgggt aacctaggcg
    19201 tttgcgttct gcaacaaagg ttttggctac aacacgggcc aagttacgga tacgagcgat
    19261 atagcctgca cgctctgtta cagatacggc accacgcgcg tcaagcagat taaaggtatg
    19321 tgaacatttg agaacatagt cataggcagg gtgtaccaag ccttcttcta atgcacgacc
    19381 agcttctttt tcaaacttat caaagttttc aagcaacatt tcttggttcg aaatttcaaa
    19441 tgaatatttt gagtgctcat actcaggctg gataaagatt tctccgtatt ttacaccatc
    19501 agcccactcg atatcataga cagagtctac ttcttgaatg taagaagcca agcgctccaa
    19561 accataggta acttccgcag tcacagggcc agttgccaat ccaccgactt gttggaaata
    19621 agtgaactga gtgatttcca ttccgtcaag ccaaacttcc caaccaagac cagctgaacc
    19681 agttgatggg ttttcccagt tgtcctcaac aaaacgaata tcgtgctcca aaggattgat
    19741 tcccaatttt tccaaagact caaggtaaag ttcttggata tttgatggag aaggcttcat
    19801 gaccacctgg aattggtggt gttggtagag acggttaggg ttttccccat aacgaccgtc
    19861 agcaggacga cgtgatggct ctacataagc tgcattccat ggctcaggtc cgatagcacg
    19921 aaggaaagtg taaggactca ttgttcccgc acctttttca ttatcataag cctgcataag
    19981 catacaacct tggtcattcc aaaattgttg caaagtcaaa ataatttctt gaaatgttaa
    20041 tttcttagac attgaatact ccttaataaa aaataatttc ttgcgacacg agtctgcctc
    20101 gtcgattata cgaggcaaga cgagttagta ctgcgtctag ctcaggcact ctagtgctga
    20161 gcgtggagca gcccgactgt aaggaggtct ctgccactga tgcagtgaaa tgttaatttc
    20221 ttagacattg tttactcctt tagtttctat atttcttttt caattaattt ctgctgaaca
    20281 aaccaatcta acaagggaaa agttttttct gtatttgccc atagatgata agaatcaaag
    20341 accgcttgta cactacctaa attttctacc aacttatcta ctatcaaaaa actgagccaa
    20401 tattcataaa aaatacttgc atagttctta tcataagtcg aagaaccaaa atcattcaat
    20461 gaatgccaac catacttctt ctgaaaaagt tctactagag attgattaca aattttttcc
    20521 gcttgaaatt cctcttctgt caagaaatac ttgcgactaa tatattcaac catcccctct
    20581 tcgaaccaga tataagagtc ataaccatca aaatcatcta aaaaatgctc cgaccaatga
    20641 gctaactcat gtcctacaat ctgtaagagg aaattttcag ataaagaatg atagtgactt
    20701 tctattgctt gagtttgctg agaacactcg tagtccatca attgatacaa atacaactct
    20761 ttccaaacag ctaaatcagg cgtcataacc attcgtctat tatttgtata ggctggaaca
    20821 gcgctttccc taataatctg tgtagcagca ttaaaatttg accaaataac ggcttgcggt
    20881 aaatcacaga ctgcaagttc attctttaaa aaagattgat aatctttcaa cttttcagta
    20941 tttcttacta caaaatcacg aaatgcagct agtggactat cgtcttttac aagataaagg
    21001 ttttccacgt ctctcctttc tttcacacaa tagactgaat aagcaaaaga accacatctg
    21061 acaatcctag gttgatttaa cctaggggcg tcagaaacgc ggttccaccc taatttatta
    21121 cttcattgtt tttgaaaata ctacagaaag cgccagctca ttattttgcc ctacttggct
    21181 cccactgtcc caagctcgct tgaagaacgc cataagactc tctttcctgc tgactattat
    21241 atcaaaaatt agaataatgt acaattcttt ttatgatttt ctagaaatcc acatcatcaa
    21301 ctcgtggagc acccgattga acagcaatcg tttttaaggt ttctctctcc tcatgactca
    21361 gttcaattcc aaaacaatca agattagctt gaatacgaga ggttgtgaca gattttggaa
    21421 gtggtaaaaa tccttctgcc aagctccagg ccaaggctat ctgagcaacc gattttccgt
    21481 gatttgctgc tatttcttgg acttgcttgc tatcaaacag ttctccttgt ccaaaaggcc
    21541 cccaagcttc caataaaatt cccttttcac gacagtaagc tacgacttga tcttgataca
    21601 cacctggcgc caagcgaact tgattgaccg caggaacgat agttgcagtt tcaagcaagg
    21661 catccaaatg atggggaaga aaattgctaa cgccgatagc acggattttc ccttcttgat
    21721 agaggtcttc catcgctctc caaacttccg cattgcgagt tttccatgcg tcattttctc
    21781 tgagcggttt tgggttcggc caatgaatca aatacaaatc caaataatcc aagcccagtt
    21841 tttctataga tttttccaaa gcttgacgag tttgctcata ggtttgctga ctattccaaa
    21901 gcttggtagt tacgaacatt tcttcacgtg gaactccgct atctttgatt gcttgaccaa
    21961 cactttcttc attctgataa atcgccgccg tatcaatatg acgataacca gccttcaagg
    22021 cttctaacac tgcacgatag gcttcttctc catccttagc cttaaaagtt ccaaatccca
    22081 atactggaat ttctactcca ttatttaatt gataggtatt catatgcttc tccttctatt
    22141 ttattcatca attatagcat aaaacatttt ttaagtagta ctatcttctg taaaaaataa
    22201 gtctgtcaaa tatctaacag acctattttt tcttgcttat tcatcacgac tatagtacag
    22261 ctcatgagct tttaggcgag tattgagcat ctctggtatg gtcacaaagg tataaccttg
    22321 atttttcaaa tactcaatga cccttggcaa ggcattgact gtcggactgt gaatatcatg
    22381 catcaaaacg atagagccgt tagctacttg atgctgaatt tctgtcaaaa tagatgcttc
    22441 atttttactc ttccagtcca gactatccac atcccacatg ataaagctca aatccaagct
    22501 attgcgaata tcatctgtaa tagcaccata aggtggacgc atgagtttag aactagaacc
    22561 cagcacttta gttagcacat cctcagtatc agtaatctgc tttttagctt catcaagaga
    22621 gagttgcgag agaatcggat ggctccaact atggtttcca acaacatgac cttcagattt
    22681 tatcctcttc accaagtcct cattcccaga aacatttttc ccaagcacaa agaaagtcgc
    22741 tttaatatca tatttagcta gggtctctaa tacctgcggg gtcgttgctg gatttggacc
    22801 atcatcaaag gttagagcga caactttttg atgtttctta tcaaagtaag attggtacaa
    22861 ggccgcatct ttttcgagta agtacgaaga ttggataaca tcaaagaaag cagatactgg
    22921 caaggctatc tcttctaaat tttcaaccac aggacttgga taaaggataa tctgactatc
    22981 cttgtaatca aaattccatg cagacaagtc ttggtcagag aagtttttta caatctgctc
    23041 actctggtct tgctctattt ttttatcctc tatgaaggag gtcaactctt ttatcagctg
    23101 ttccttagcc ttactagcat ctgaaaacag ttggtcaagt gtaaaaggtt gcccatcttc
    23161 tgttaagtga acctttccta gactggtctt ttcagtctct tcaatcttaa aagcaactaa
    23221 atcatagatt tgtttggtca cattacgatt aacgactccc tttaaacctg actcttcttg
    23281 ctctgtatag tagaaaacaa gattgtcctt actttctagc ttgtccttaa catcttgatt
    23341 tatcagctcc ctaacagagg aaatcacttt ctccccttgg atagggtaat aggcaatcac
    23401 ttcggcttgc cccttacgaa aatgcttctt ctgatttccc tcactcaatt gatcatcttt
    23461 ctcttttttg agcgattcaa tcttttgctc aaaacttttt tgctggtaga tcttaaaagc
    23521 aataaatccg cctaataggc aaatagaaat tgctaaaata ccaatcaaag ccaataatac
    23581 atgtctcgtt ttcccgtgtc tgccacgtcc cagtctactt ttatccatat ttatatcata
    23641 acaaatttga gaaataattt caatgataca atttggcaat ccacaattcc tctagcaagc
    23701 caaagcatct tacatcaact ccaacatccg tatctgtaga gattatgtag ttttctatct
    23761 atggaatttc tagtggattg aaactagaat agtagaccta tacttctaaa acattgttag
    23821 aaatcgattt gactgtcctg ttcgatttgt cctgttttta tttcatttta ctataaaact
    23881 atgaaaagct aggtcatctg agaagtaatt actatcgaca attgcgatag cgtatactgg
    23941 tgcgacgtgt ttgtagctct ggtgggtatc accttcacgt tgacggattt tttcaaggtt
    24001 ttgattaacc tgactgcaca gataagccca taggcgattg atgaaaaaat tttgatgatg
    24061 aacctgaatt tcgatgataa cttgggttcc attatctaac tccgccaaga cgtcgtctat
    24121 actagtatag aaatcctgcg ctgagtaggg catggaaggc aagacatgaa tatcgcttcc
    24181 ctccaaaatg gtcacatttt ttgctggcaa gtccagcata tcgcgaataa attgacaagt
    24241 cattggtcgg gctaatgccc ggatgtctta gaatcatcct tttcctcctt ctatagtgaa
    24301 ttgaaataag atgt
//
LOCUS       DAVKAO010000025        23733 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKAO010000025 DAVKAO010000000
VERSION     DAVKAO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23733
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.025"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(60..509)
                     /locus_tag="VWX16_001041"
     CDS             complement(60..509)
                     /locus_tag="VWX16_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9158413.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(511..1269)
                     /locus_tag="VWX16_001042"
     CDS             complement(511..1269)
                     /locus_tag="VWX16_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9158414.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1384..3363)
                     /gene="pknB"
                     /locus_tag="VWX16_001043"
     CDS             complement(1384..3363)
                     /gene="pknB"
                     /locus_tag="VWX16_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9158415.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3360..4100)
                     /locus_tag="VWX16_001044"
     CDS             complement(3360..4100)
                     /locus_tag="VWX16_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9158416.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(4115..5428)
                     /gene="rsmB"
                     /locus_tag="VWX16_001045"
     CDS             complement(4115..5428)
                     /gene="rsmB"
                     /locus_tag="VWX16_001045"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9158417.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREDWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5421..6356)
                     /locus_tag="VWX16_001046"
     CDS             complement(5421..6356)
                     /locus_tag="VWX16_001046"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9158418.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6369..8765)
                     /locus_tag="VWX16_001047"
     CDS             complement(6369..8765)
                     /locus_tag="VWX16_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9158419.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPMEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGIEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRARYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8831..9145)
                     /locus_tag="VWX16_001048"
     CDS             complement(8831..9145)
                     /locus_tag="VWX16_001048"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9158420.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9170..9796)
                     /gene="gmk"
                     /locus_tag="VWX16_001049"
     CDS             complement(9170..9796)
                     /gene="gmk"
                     /locus_tag="VWX16_001049"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9158421.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQERLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(9926..11539)
                     /locus_tag="VWX16_001050"
     CDS             complement(9926..11539)
                     /locus_tag="VWX16_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9158422.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11821..12051)
                     /locus_tag="VWX16_001051"
     CDS             complement(11821..12051)
                     /locus_tag="VWX16_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9158423.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDKFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMDKIRAGH"
     gene            complement(12289..13386)
                     /locus_tag="VWX16_001052"
     CDS             complement(12289..13386)
                     /locus_tag="VWX16_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9158424.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVNILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLTDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKVDQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13396..14142)
                     /locus_tag="VWX16_001053"
     CDS             complement(13396..14142)
                     /locus_tag="VWX16_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9158425.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEIHEERTYELLTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14245..14598)
                     /gene="rsfS"
                     /locus_tag="VWX16_001054"
     CDS             complement(14245..14598)
                     /gene="rsfS"
                     /locus_tag="VWX16_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9158426.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(14611..15111)
                     /locus_tag="VWX16_001055"
     CDS             complement(14611..15111)
                     /locus_tag="VWX16_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9158427.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEEHFADVL
                     DYKHIF"
     gene            complement(15112..15705)
                     /gene="yqeK"
                     /locus_tag="VWX16_001056"
     CDS             complement(15112..15705)
                     /gene="yqeK"
                     /locus_tag="VWX16_001056"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9158428.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(15705..16334)
                     /locus_tag="VWX16_001057"
     CDS             complement(15705..16334)
                     /locus_tag="VWX16_001057"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9158429.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GPSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16386..16697)
                     /gene="yhbY"
                     /locus_tag="VWX16_001058"
     CDS             complement(16386..16697)
                     /gene="yhbY"
                     /locus_tag="VWX16_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9158430.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(16912..18018)
                     /gene="yqeH"
                     /locus_tag="VWX16_001059"
     CDS             complement(16912..18018)
                     /gene="yqeH"
                     /locus_tag="VWX16_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9158431.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(18021..18611)
                     /locus_tag="VWX16_001060"
     CDS             complement(18021..18611)
                     /locus_tag="VWX16_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9158432.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(18563..19471)
                     /locus_tag="VWX16_001061"
     CDS             complement(18563..19471)
                     /locus_tag="VWX16_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9158433.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(19528..20088)
                     /locus_tag="VWX16_001062"
     CDS             complement(19528..20088)
                     /locus_tag="VWX16_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9158434.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20227..21433
                     /gene="sstT"
                     /locus_tag="VWX16_001063"
                     /pseudo
     CDS             20227..21433
                     /gene="sstT"
                     /locus_tag="VWX16_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21492..22445
                     /locus_tag="VWX16_001064"
     CDS             21492..22445
                     /locus_tag="VWX16_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9158435.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            22588..22968
                     /locus_tag="VWX16_001065"
     CDS             22588..22968
                     /locus_tag="VWX16_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9158436.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            23067..23288
                     /locus_tag="VWX16_001066"
     CDS             23067..23288
                     /locus_tag="VWX16_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9158437.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            23305..23619
                     /gene="trxA"
                     /locus_tag="VWX16_001067"
     CDS             23305..23619
                     /gene="trxA"
                     /locus_tag="VWX16_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9158438.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
BASE COUNT         6444 a         5542 c         4685 g         7062 t
ORIGIN      
        1 gaaagcgttg ttaatttatt tatctcattt ttcagaaaat tcttttattt ctgtaaagtc
       61 tacgatactc gatgtgtttt tatataatga tacagtctga gaatcactgt tccgctagcc
      121 attccaatag agattaccaa agccaacatg acaaccaagg tcgcacttgc cagtgcttta
      181 ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg agagataacc tggaaccagc
      241 ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct tataaagaat acttaaaatc
      301 tggctgacac aagaaccaat aatggctgca atgaaggtag ctacaatgac attggtcggt
      361 tccttgagca agagatagat tagccagaca gtcatgccca aaatccctcc aggtaagagc
      421 atagaccgtt gcacattaag tacgattaaa aaagtgataa tggcaagaaa acttgctact
      481 gcttgtaata aaaaggttgt tagtgtcata ttagttcatc aataccaagg cgacagaagt
      541 tcctgcccct aaagcgaggg taatgagcag ggattcaaac atcttactca taccagagtt
      601 tatgtggttg gtcataatat cacggaccgc attggtcaag gcaatacctg gtacaaacgg
      661 catgaccgca ccagctataa tcaaatctgc cgttgaagga aaacctgtgt agcgagccca
      721 aaactgggca attatcccaa agacaaaagc tccagcaaag gctgtcacaa agggaattcg
      781 gataaatttt tccacataga gggaaaaggc aaaaccaaat aaggtcgcca ctcctgcccc
      841 aagtgcgtcg tagatatttc cgctaaacat aactgaaaag aaaggagcac taaaggtcgc
      901 agccagagtt acctgcaact tagtataggg aaggggttga gcttgcaagg ccgtcaattg
      961 cttaaaggct gtttctaagt caatctgccc cccaactagc tgacgagaaa tctggttcac
     1021 atcgcagact ttttcgatgt tataagaaga ggaggtcacg cgcttcatgc gcgaaatatt
     1081 ggtattttca atagagaaaa agatagcggc aggcatggca aggacattgc aatccacaat
     1141 cccctgcgaa tgcgcgattc gaatcatggt atcttctaca cgatggattt ctgagccact
     1201 tttaaggaga atagtccccg ctagcataat cacatcaatg acggcattta attcttttga
     1261 ttcttccatg ctttcctcct tttatcaact ccctctattc tatcacaaat ctggactcaa
     1321 aaaaaatctt tgccatgaaa tcatgacaaa gattgattac tcattttgat tatccatctg
     1381 cttttaagga gtagctgaag ttgttttagg tttgtagatt gaaatcttga ctctagtctt
     1441 atttaggtct accttttcac ctgctctagg actttgttca acaaccatgc cttctacact
     1501 acctgcaggc gctgtcgtca cttctacaac ttctatatta gcttccttaa tcccaacaat
     1561 ttgaatcaaa ttgttcttag taaactccaa gctggaacca atgtaactcg gcatggcaac
     1621 acttgtaact tttttagcta ctgtcaagac aatttgagta ggtttactca catcataagt
     1681 cgttccggca cctggacttt gtttcataat cgttcctggt tcgctttcgc tggactcttc
     1741 ttcctctatc ttaatcaaat tctcaggaac cttcttctgc ttgagttctg agattacttc
     1801 tgtagagttc cgtccaatat agttccctaa ttgaatcgtc gtagcttttt tagctactgt
     1861 caaaacaatt tgagttgcct tgctcaagtc ataggtcgta ccttctggta gactttgctt
     1921 caggaccgtt ccagcctcac tctcattgga ctcttcttcc tcaattttaa tcaaattatc
     1981 tggaactttt ttctctttta attccgcgat aacatcagaa gatttccggc cgacataatt
     2041 actaatttgg aaggattgtt tgcctgatga gacaaccaga ttgattttcg ttccttcttt
     2101 tcgaccagtt ccagcgccag gatctgtacg gataatccgc ccttcttcca ccttttcact
     2161 agcctctgtc ttctcctcac caatctcaaa attggctttt ttgagcgttg ccttggcctc
     2221 tgcaactgtc tgacctgcca catctggaat ggcaatggtt gcaggagttc tggatagtat
     2281 ccaaataaga gaagctgcca ccaatacaag gctggccaac aaaatcaggt aacgcatctt
     2341 aaatctatgt tttttcggtg cttgtggttg gtaagtttcc tctgtcacag cctggcttgg
     2401 gtttttgatt gatttgtgtt ctgtctgcgc ttgaacctta ggaatagatg tcaaggtact
     2461 ctgagaaacc ttcggcaagg tcttggtatc tgccttgctc gtttcatcaa agattaactt
     2521 actttcattt ctacgattgt aggacaagct actagacaag tccacataca tctctgaaac
     2581 cgagcggtag cgattggtca actttttagc agttgccttg ataataacat tttctaaagc
     2641 ctgaggtaca gatggatttt ctgcaataac ggacggcagg ggtttctgga aatgctggag
     2701 ggcaatggtc accgcgctat ccccgtcata agggatatgg cctgtcaaca tctcatagaa
     2761 aataatcccc atggcataga tatcactctg cacagtcgcc ttagaaccac gcgcctgctc
     2821 tggtgacaag taatgaactg agcccaacat cgagttagtc tgggtcagac ttgtctctgc
     2881 aaaggctaca gcaatcccaa agtctgtgac cttggcagtc ccatctggtg tcaaaaggat
     2941 attttgaggt ttcaagtccc tgtgaacaat tcctcgagta tgggccaagc gcatagccaa
     3001 gagaatttgt cccatgatac ggacggcttc ttcattagaa agaggataat gttccttgat
     3061 atagcgtttg aggtctagtc cagcaacata ctccattgca agatactgtt gaccgtcttc
     3121 ctcaccaata tctgttatcc gaacgatatg aggatggtct agatctgcca tagctctcgc
     3181 ttcacgctga aaacgagcta cagctatcgg gtccgtctgg tagttggtcc tcagaacctt
     3241 cactgccact tcttccccat ctaagattaa gtctttggct aggtagacat ccgccatgcc
     3301 tcctcgacca atctgtttga caatccgata gcgtccggca aaaatcttgc cgatttggat
     3361 cattcttcat cctcctcgtt catagaaaca agggcaaccg taatgttgtc taaacctcct
     3421 gcattgttag caaaacgaac aagtgtctcc gttttatctg ctaaaggaat atcactggtt
     3481 acaatatcac gaatctcact gcctgaaatc atgttggtca agccgtcact attgagcaag
     3541 agatagtcac ctgactcaag gataactgtc ccaaaatcag gctgaatttc atctttttgc
     3601 ccaatagact gggtgataat atttttttgt ggatgagctt ctgcctcttc tggtgtcaat
     3661 tgaccagcct tgagcaattc attaaccaaa gaatgatcgc tcgtcaactg atggtattct
     3721 tctccacgaa tcaagccgat acgcgaatca ccaatatgag catagatagc ctgattatca
     3781 ataatagcaa ggacttccaa agtagttccc atgcctctgt aagcttcatc ctgaccaagc
     3841 tggtgaatct tttgattttc aatttctagg taatgggcga accattcacg cacttcattg
     3901 actgtatcga tctgggtatc aacccaagct acacccaggt ctgtcaccgc catttcacta
     3961 gcgatattcc ctgcgcgatg acctcccatc ccatcagcta aaataatcat ggtacgtcca
     4021 gctctattga catagtggtt gacatagtct tggttatttg ttcgtttctg accaacatct
     4081 gttaataatg aaatttccat gtgtcagttc cttcctaatc cgatatcttg cgaaattgac
     4141 tgatgaagaa tccatcactt ccatacaatt caggtgtaat gaggatacag ccgtctttca
     4201 tgatatcctt acattcatgt tctagtttta cctgctcgaa ctcgggatga ctctctaaaa
     4261 aggccttaac gacttgaaaa ttctcctctg agacgatagt gcaggtgcta taagttatta
     4321 taccaccttt gcctagtatt tgacaaacac tacctaatat ttctaactga atttcctgca
     4381 aggacgcgaa atctgccgtt tctttattgt atttgatatc tggttttcgg cgcaaaagac
     4441 cgattcctga acaaggagca tccaccaaaa tcttatcaaa ggaatcctgg tcaaaaaact
     4501 catgcacctt tctggcatcc aatttttgag tttgaacccg atcttcaact cccagacgtt
     4561 gggcattttc ttgaattaaa tccaacttgt ggtcgtacaa gtccagagca gtaacctgac
     4621 ctgtcgtaag ataagaggct atatgggctg ttttcccacc tggagccgca caggcatcaa
     4681 gcactcgctc atcaccttgt aaatcaagcg tcggagcaac cagctgactg gactcgtctt
     4741 ggatggtaat ggctccatcc gcaaacaaat tatgccctgc aaaatgcccc tgctccttaa
     4801 ccagaccagt ggttgctaaa agggaattat tcgcctccaa caaggcttgg atttcctctt
     4861 ttcgacttag gtctgttaca cgaatactgg ctttgtttcg cactaacagg ctttcaaaga
     4921 tggcttttgc tctctcctct ccgtattctt ccttgagttt ggcaactagc caaactggga
     4981 gagaataggc aatggagtca cgcttgtttt ttcgcttgat gctagcaata tctggccagt
     5041 cttcacgcaa gatacggcga aggacagcgt tgaccaattt ttcactgcct tttttacgga
     5101 gtttggccaa ttccactgct tcattgacca cagcatgatc tggaatcttg tccaaatagc
     5161 ggagttggta ggcactcatg agaagaagga catagagcca gctgtctaac tggtctctgt
     5221 cttcgataaa gtgggatagg taccattcca gagtcagttt acgggctacc gttccataga
     5281 ctagctcggt cactaagccc ttgtctgctg cagaaagctg actccccttg aggtgtttat
     5341 ttaaggcgat atttgaatat gcttggttca caaaaacatc ctctagcact gctagagcta
     5401 aacttctagc cgtttctact ttagtcacca aatcgttctc ctacagtcaa tgtacgtcca
     5461 actccgttga ggaaggaagc aatgtccatc ttaggcttac cagctggctg cacttgtttg
     5521 agggatagag ccccttcagc cgttgcgaca atcaattctt tcttgccgat agagagaatt
     5581 tcacctggat ttccctgacc ttctactggt agggcttcat aaatcttaaa gcggtcgccc
     5641 ttaaggaaag tatgggcaac aggccagggg ttcattccac gaatttggtt aaagagttga
     5701 cgattggttt tgttccagtc cagtttttct tcctctggct ttatatttgg agagaaggta
     5761 acctgactcg tatcctgcgg ttcaggtttg atatcaccag caatataggc aggcagagtg
     5821 tccaaaagca aatcacgacc aactagcgcc aatttttcaa acaaggtgcc aacattgtcc
     5881 tcatctgtga tcggaatgct gcgacgagaa atcatatctc ctgcatccat ttccttaacc
     5941 atttccatga tggtcacacc agcttcctca tccccttgaa tcaaggcata atggataggc
     6001 gcaccaccac ggtgtctagg aaggagggag gcatgaacgt tgacagcaaa gtccatgcta
     6061 tcaaggagtt tgcttgggag aaactgccca aaagcagcag tcacaattcc atctgctcct
     6121 agcttcataa gatcttccat ctctggactt ccagataatt tttcaggttg gtagatagat
     6181 agtcctgctt ccttggcagc ctgcttgact ggggtttctt ggataacttt tttacgacca
     6241 acagcacggt ctggctgggt cacaacggct agaatttcgt aacggtcatc tgtcaaaagt
     6301 ccttttaaga ctgttgctga aaagtcgggg gtccccataa agattagttt tgtcatatct
     6361 tctccttctt ataaaaattg ctgcggctca tggtcaatgc tgagacggag ctcactattt
     6421 tcccgttctt gagtcaaggc taaaacctgg ttgagggtcg gccccagctc atcctctaaa
     6481 cggtatttaa ttaaaatctg gtaatgatag aggttgtggg tacgggcaat cggttttggc
     6541 gttggcccca gaatgggact ggtctctgac aagcctgacc gcaaaatgtt catgacttca
     6601 taggcacgtt tgaaaacctc ttcttctttc ttgtgagaaa gggtaatacc aatcgtgaaa
     6661 tagtaaggtg gatagccgag ttgtcgtctg attcccattt cataggcata aaagccttcg
     6721 taatcttgat ccttggcaaa tcgaatagca tagtgctgcg gattgtagga ctgtatcaag
     6781 acttgacctg ccttttcagc acgacctgct cgacctgcca cctgagtcaa gagctggaag
     6841 gttctctcag aagaacggaa atcaggcaga ttcaaggccg tatccgcatt tagaactccg
     6901 actagggtaa cattgggaaa atccaaaccc tttgcaatca tctgagtacc aagtaaaata
     6961 tccgcttccc ctcgcccaaa ctggtcaagc aaggcttggt gactgccttt ctttcgagtc
     7021 gtatccacat ccatcctcag aatgcgagct tggggaaaga gttctgctag ctcatcataa
     7081 gccttctgag ttcccgtccc atagtaacga atactgcggc tcttacagtt aggacagacc
     7141 tgaggaatat ccttcgagaa accacaataa tggcagttca tagtcttggt atccatatgc
     7201 aaggtcagag aaatatcgca gttgggacaa gtatccaccg tcccacactc ccgacacatg
     7261 acaaagctag aataaccacg gcgattgagc atgagaacca cctgctcttt tttaaccaga
     7321 cggtcttgga tagcctctag caaaggaggc gtaaagtttg acgtctcatt ttgtccgata
     7381 tagtctcgaa agtcaatcac ttgaacctca gggattgtag ccaaaggatt ggcacgttgg
     7441 gttagacgta agtgttgata gacgcctttg ccagcacgtg cccggctctc taagctcggc
     7501 gttgcagatc caagtaccag agttgcttga ttataccgag cccgtaaaat agctacctct
     7561 ctggcatggt aacggggatt gctgtcctgc ttataagccg cttcatgctc ttcatcaata
     7621 atcatgacac ccagattttt cagaggagca aagatagcag atctggcacc aacaacaact
     7681 tgggcatcgc cacgctccac cttgcgccat tcatcatact tttcaccatt ggataatcct
     7741 gagtgaagaa tggctacctt gtccccaaaa cgtgctataa aacgctcggt catctgagga
     7801 gtcaaggaaa tctcaggtac cagcaaaata gctgtcttgc ccttatccag ggcaccttgg
     7861 ataatctgca agtaaacctc ggtcttccca cttcctgtaa tcccttgaag tagaaaggga
     7921 ggttgagaac tgccaataga actcacaacc gcatcacgcg cctgtctttg ttctggattt
     7981 aactccaaag gtctacttgc ttcaattcct tcaaaataag cagccgagcg tcgaacttcc
     8041 ttttggacta tggtaacagc accttgatcc acaaagaagt tgacttgctc tcgcgagtag
     8101 gactctaaca agctagccaa ggaagcgctc tctggatgag acagcagata atctctcagt
     8161 tccaactttt tcttggcacg tgtagaaatc tcaatacctt ctaattgagc atggtcaacc
     8221 tcataccaag actgggtctt gaccttcttt tgatcgactg cctgatattc cagaccaagc
     8281 aggccttttc tagtcaaacg catcatttca gcttgcttgg caaggtctag tgaagaaaag
     8341 gctagcgaat cttctgaacc aaacaggcgc actcgttctt cctgactcaa gccttccata
     8401 ggatagagaa tcttgtcata gctagaattc agaaaccctg gaagcatggc cttgaggata
     8461 gagattttgt aggagaagac agatttgcgt aactcctcag ccagccagag ttgttctggc
     8521 gtgagaacag gagaaaaatc cagcacctct gcaatatctt ttaaatcttg ctccatctct
     8581 tctccatctg attgggactc caaaccaaga acaatccctt gaatcaggcg attaccctta
     8641 ccaaaaggca catgaacccg catcccaact tccagcattc cctcaaattc ctccggaatc
     8701 ctgtaactat agggctggtc cgtctgcatc aagggcacat ctacgataat cttagctagg
     8761 gccatcttct cacctcctcc ttgtcagtac attcttgcaa tagaaaaaat aagattgagt
     8821 ccccccaacc ttaaattttt tcaccatctt ctttttcttt agcaatttgc tctttgattt
     8881 tcttttcttc ttcttctttg cggcgttttt cttcttcgat acggcgacgc actgcttcac
     8941 gttttccttc tggatctggg tgaattgtaa cgtttcctga ttcgatttct tctaaagcgc
     9001 gaagagttga tttttcagac ttgaaacctt gagttgctgg ggcacctgct tccaattcgt
     9061 gggcacgttt tgcttccaag attacgagtg aatattttga aggaaccttg tcgagcaagg
     9121 tatcaataga gggttttaac atcatttgct tgtacctatt ttctaaattt tatcgggtag
     9181 ttggagattt tggtaacatc tcctgatagt gaccaatgac acgatccaca cagaagtgtt
     9241 ctgcttcaat cacacatttg acacgttcag cagctagggg tacctgatcg ttgacaatcg
     9301 cataatcata ctcacgcatg agggcaattt cttccttggc cttttcgatt cgttgggcaa
     9361 tcacttctgc actatctgtt ccacgaccta ccaagcgttc ttgcaattca tccaaatctg
     9421 gtggtgtcag gaagataaag acagcatctg gaaccttttt cttgacctga agagcaccct
     9481 gaacttcaat ttcaaggaaa acatcgattc ccttgtccaa ggtttcattg acataggtca
     9541 gaggagttcc atagtagttg ccgacatatt ctgcgtattc caacatctgt ccttgacgaa
     9601 tcagctcttc aaattcttca cgagtacgga agaaatagtc aacaccgtcc acttctccag
     9661 gacgttgtgc gcgtgtcgtc atcgatacag aatattgaaa ttggttttca gaactctcaa
     9721 aaatctctct tctaaccgtt ccttttccaa cccctgaagg accagaaaaa acgattagta
     9781 agcctcggtc tgccattgtg tctcctttta gtcaatctgt gaaataacat ttctctagaa
     9841 taatggcaaa aagccagatt atcctttaca gtctttctat ctagtgtaac aaaaaagcag
     9901 taatttttca actgctcttt cttatttatt tagcataatc tactgcacga agctcgcgaa
     9961 tcacggttac cttgatattt cctggataat cgagattgtt ttcaattttc ttacgaactt
    10021 tgtgagccaa gattgtgact ttgtcgtcct tgatttttcc tggattgacc atgatacgaa
    10081 tttcacgtcc tgcttgaagg gcaaagctag tttgcactcc ttcaaagccg ttagcaattt
    10141 cttccaaatc atggagacgc ttgatgtagc tttcaagaga ctcactacga gcacctggac
    10201 gggctgcgct caaggcatct gctgcagcga cgataactgc tatcacgctc tcagcttcaa
    10261 catctccgtg gtgactagca atcgtattca ccacaactgg gggttccttg tacttacggg
    10321 ccaattccat accgatttca acgtggctac cttcaacctc atggtcaatg gctttcccga
    10381 tatcgtgaag gaatccagca cgacgggcaa gagccgcatt ttcaccaagt tcgctcgcca
    10441 tgataccagc caacttagca acctcaatcg aatggcgcaa aacattttgt ccatatgaag
    10501 tacggaactg caaacgtccc ataatcttca tcaagtctgg atgaaggttt ggcgcaccaa
    10561 tttcataggc agcagcctca ccgtattcac gaatcttatt gtcaatctct tgacggtttt
    10621 tctcaaccaa ctcttcgata cgagctggat gtatacgacc atctttgagc aacatttcca
    10681 tagtcatacg ggcaatctca cgacgaatcg gatcaaatcc tgacaaggtc accacttctg
    10741 gtgtatcgtc gataatcaca tcgacccctg tcaaactttc aaaggtacga atgttacgac
    10801 cttcacgacc aataatgcgt cccttcatag tatcgtctgg cagatgaact gttgagtttg
    10861 ttgactccgc tacatattca ccagcgatac gttgcatagc ttgaaccaag atgtccttgg
    10921 ccattttgtc agaacgttcc ttgacctctt gctcagcttc gcgaatgcga ctggcaatct
    10981 ccctggtcaa gttttcctct gtctgagcca agataatatc tcgtgcttct gcctgagaca
    11041 gagcaccaat acgctctagt tctgcttctt tttgtctttc gacttcctct aattgctctt
    11101 cacgcgcatc aaggtttttc gctctatcag aaatactttg ttctttttgt tcaagtgttt
    11161 gttctttact cgtcaaattg tcgtccttac ggtcaaggct agtagctctc tctgtcaaac
    11221 gactttcgat ttgtttgagt tcttgacgtt ctgatttgaa ttcagcgtcc acttcttcac
    11281 ggtattttct ggcttcttct ttggcctcca atagtgcttc ttttttaaga gacttgcttt
    11341 cacgtttggc ttcattaaca agtaaatccg cttcacgctc agcttgtcca cgtaaattag
    11401 ttgcttcttg ttcagcattt aaaagcatca actctgcagc ttcctgagat gatttcatct
    11461 tagctgagat gctgacatat ccaatgacta aaccaatgat gacggcaaaa acagcaatcg
    11521 caagcgacat gatttccatg tttttacctc attttattgt tattccgaat gacatacatt
    11581 cttttacatt ctaccataaa aaagtgattt tcacaaacct aaaatagaat atgttttggg
    11641 gaaattggag tttaaatgcc tataatttat tggcacaatc tctatttctt agaaatcgtg
    11701 gtttagtaaa gatttgagaa aaagcctact tttcagtaga cttcctaata atcgttagaa
    11761 tgcaagaaat ttgtttctaa caacagcaag atttcactca ggtattctct gtaaattaga
    11821 ctagtgtcct gcacgaattt tatccattcc tcgcaaaacc ttgtctgata actccttatt
    11881 ttgggcgatt ctcagggttt cctgaatact gtcccattcg ctctttgaaa gaactacaat
    11941 gtcctcatct ggatttttat tgaccaccgt caaaggctca aatttatcat ttaccttctt
    12001 catgtagtcc tttaaatgat ttcggaatgt tgagtaaagg actgcttcca taaccacacc
    12061 tcgttttagc tcttttccac tattatacac gaaaagaaag aaattgtcag gaacttgtac
    12121 aagattttct tttctatcta tttatactca atgaaaatca aagagcaaac taggaaacta
    12181 gccgcaggct gtacttgagt acggcaaggc gacgttgacg cgatttgaat ttgattttcg
    12241 cagagtatta ttcgtaaaaa atctcaaaaa gcctaccttt cggtagactt agtttgtttc
    12301 tattctaatc ggcactcttc caaaattttg ctctgctata cttggctttc ctagttggta
    12361 aatctggtca accttttgag tcatagcatc ccaaggttct ttgccaattc ggctgactag
    12421 attgacctgt cctttcagag acttgagatg ttgcctgcct ttttcagtaa atccaaggac
    12481 atgaatggct tctggcaagt cactttctct agcctgcatc aaaatatagg tcaataggcg
    12541 tctgacacgc gccttggtgt aacgtttggt agtcactaac tcgaccaatt cttccacaga
    12601 ctgggctgtt ttaatagctt ctttaatgcg cacagccatt tcttgattga cctgatagat
    12661 ggtggttagg tcgggatttg acaagatttg atagcggagc aagggaaaat agtcttccca
    12721 gatcacctta ctggcctgct caaatagggc aacagaaggc ataaaacgtt ctaagaaatc
    12781 ttggtccttc tgatgttgac ggagggctgt cgccgaggca aagtccacat ctttattcac
    12841 agaatggtaa cctgccccct gacgctgaat cggatgcagc ttgatattcc gtcctgcaac
    12901 cgccttggca taggccagag caagaacatg attgggggta ttacctgaaa aatcaagacc
    12961 agcaaattcc ttccacatag cttgggtttt ctggggatag gaaagggaat cagtcagatt
    13021 ttccacaaat ttctccatct cagcaccttt ctctgtgtat aagtcagcga ttttctgata
    13081 atctcgaact tcctctgtcc caaaaactag actatcaatg cccaagcgat ccaagatatt
    13141 cacagctcct tgaccaaaga aatcagctgc ctgaacactg actaaaaagg gcaattccac
    13201 taccaagtct gcgccatttt ccagcgccat ctgggccctt gtccacttat ccacaatagc
    13261 aggctctcca cgctgcatga aattccctga catggcaaca attttcagtc cctctgcctg
    13321 atccagcagg tatttatggc cattatgaaa aggattgaac tccgcgataa tacctgtgat
    13381 ggtcatgcta atctcctact tctgcgccac aaaaaaccaa cgggtgctag tttctgttgg
    13441 ctccttatcc tcaaagtctg catagagttt gaaggactta aatccagcct gttccagcaa
    13501 gatatcatag gtcaagagct cataggtcct ctcctcatgt atttcatcgt ggcgactaaa
    13561 ggaaccgtca gcctccttga taaagaaagt cagctcatgc acgatggagt gaggagcttc
    13621 gtcctcatat gtatcccaaa gcatggcaaa atcttccgca ttttcatggt aggaatagcc
    13681 agggaatact tcatctgtct ggtaggtcga gtgcacgtca aagatgaaaa ctccctcttc
    13741 attcagcgca ttgtacacgt ccttaaagac gtcccctact tccacctcat cctgcatata
    13801 gcagatagag tccgaataac acgtgacaaa atcgtatttc cctgccttgg acaaatttag
    13861 catattacct tctataaaag caatcttttg cttggctgaa gttgctctct tctccgcaat
    13921 cttcaacata tccgcactca agtcaagtcc agtcacatca aaaccagcct gagagaagcg
    13981 cactgattga attcctgtcc cacaagccaa ttccaagagt ttctttctct ccttggtctt
    14041 aggcaaatga cgcagagaaa aattcgtcca tttgtcgtat aaactatcgt ccatgaccgc
    14101 atcatagacc gccgcgaagg tttcataagt cgccataatc atgatactaa gagctccatg
    14161 gtaaacacca ctagcccttt acctttctat caatttttct aaaataagca aagaaaccca
    14221 gttgactaca actgagttca tcttctaagc aagagcttct gaaatatcta ctgaattcgc
    14281 ctcatgccat agcttctcta ggttataatg ggcacgcatt tcttctgaaa agatatgcac
    14341 gacaacagca ccgaggtcca gtaagaccca gcctccagct gcatcgcctt cgacatggct
    14401 acctttaaag cccgcttgag ctactttttc acggatatta gctgcgatag cgtccaactg
    14461 acggctattc attgagctag tgatgacaaa gtagtccgtc acactagtca aatcttgtac
    14521 atcaagtgcg aggatatcct ccgcacgttt ctcatcagcc gctttcacga ctagttctag
    14581 taattctttt tcgttcattt aatcctcttt ttagaaaata tgtttgtaat ctaggacatc
    14641 tgcaaagtgc tcctcccaaa tgtcctcata aaattcatta atcgtttctg ctggaatgtc
    14701 atccccatca aaggttgtga cagcaccttc tggaacaata agctgatagc catattcaaa
    14761 ggcaaccttg acagaggtat ccacacaata ttctgtctgc ataccacata aaactaattt
    14821 ttcaatcccc tgtttatcca agtattcttt taagccagtt tctttgaaaa tactgttata
    14881 cttcttctga aagacctttt cagcaggttt tcgatttaaa agcgcagata actgccaatc
    14941 ttctgatgtt tgagcttcag agttctcaat atgttgaaca tagataattt caatattctt
    15001 gcttctagcc tggttttgta aataagaaat ttttcccaat agactttttg tctggaaccc
    15061 agtttctact aaaacattct gaacatcaat gattataaaa gccgtcttca tttagttctc
    15121 tttcaaatag tgcacaaagg cgttataggt ttcaagggtt tggggataga tggggaatcc
    15181 ctgatgagct agatactcca cggtacgagc tgtttcgtag gccaccgcct tattgagcga
    15241 tagacttgca atctcacgcg ccacatctac tccaggaaag gctcgattgt gctcgatata
    15301 gtctgcgacg tagatgacct tatctaggtc tgtcatctga ccagcaccga ctgtatggac
    15361 ttcaatggct cgcaggattt ctgaatcatg caaatccaaa tcttcctgaa ttttgtaaat
    15421 tccgaccata ccatgccaga cattattacc ccagtttttg aggtcgaaat ctagctgata
    15481 acggtcaatc aagtctagaa attcctgatc tgacaacttt ttagcatagt catgaagaag
    15541 acctgctaga cttgctttct caacatctac accaaagcgc tgagctaatt ccatagcggc
    15601 acgctccaca cctagacaat gggttagacg tttctcaggt agaagctctg ccattttttc
    15661 caacaaggcc tcacgggagc agttgatata gtcttgatag gacatcagta gagcccctcc
    15721 ttctcgatgt agtctagcac tggctgaggt aagagaaagt tgggtttccg accttgggca
    15781 atgaagtccc gcaccatgct ggacgagata tccatgagcg gtacgtccac ccagataact
    15841 ggataggaag gccctacctt gtagcgtgga cgctgaaccc ccacaaactg aaccatgtca
    15901 accagttcat caattcggta ccacttaggc agatagtcaa ccatgtcggc accgatgata
    15961 aagtagtaat ccgtatctgg attcttctct gtcaaaatct tcatggtgtc gtaggtgtag
    16021 gaaataccct tgcgctccaa ctcaatggtt tcaatgacta ggccgtcaat tccctcaatt
    16081 gccaactcaa gcatcttgag acgatggtgt tcagggatgg tttccttttt atcaacgtga
    16141 ggaggttggt attcaggcat gagcagaact tgatccagtc ccaactgttg ccgtacttga
    16201 tccgcaacaa tgagatgggc attgtgaaca gggttaaaat tcccccctaa aatcccaact
    16261 tgtttgcgtt ttttctcctt gatttctggc tccaactcta ccttggtaaa gggagtcaat
    16321 agttcgattg tcataggcta gtccccttta tttctctata aaaacagtta tttggagttt
    16381 cgatcttaga tttctttgac tttcttagaa atcttgcgat tttctttctt gctagattgt
    16441 ttaaacaaaa tcaagatgcg tcctattttt tggactgtat ctacaccgat ttcttcttcc
    16501 aaaatttcag ctacttcgtg gatgttttca tctgtgtttt gtaagagagt aaccttgatt
    16561 aattcacgcg catcaagagc ttgacggacg ctggttttga tttggtcgtt gagtccattt
    16621 ttcccgattt ggatgatagg tttgagggtg tgtgcctggc tgttgaggaa ggcacgttgt
    16681 tttgatgtta atgacataat ttctttaaaa gagtttcttt ttatactttt ctgaagtggt
    16741 gacggacgtc agcaaagtcc ttcggacttt catgactaaa atttgagcct aaggtctcaa
    16801 attttccgca gttggtacaa tcacttgtac caacttacac cacagcgaaa agtattctct
    16861 atggggctcg ccttgctcgc ccaaattcag acaacccttt cctttctgtg cttaaataat
    16921 tgcttttcgt gtgacgacgg cgacgccttc tggtgcccag acggcgactt ttgctgtgcc
    16981 tgttacacga atccatccta ggcctgagat gactaggtct gtcttatctt taatggtaaa
    17041 gacatgctgg actagccttg ggaaatcttc tttttccttg ctatttggtg gtgtcagaag
    17101 agttcccagg tgcttatcgt agaaagcact agctccttca agcttgctac gatggagttt
    17161 gagttcatta tcaaagaaag cagtaaatcc ttgcttttct cctgctataa agtcaaagcg
    17221 tcccaaacca cctaaaaata gggtttgctc aggattaagc tgataggtct taggcttgat
    17281 ttccttttta ggactgacat acttgaggtt tttggccgtc aagtagtgag ccatctggtg
    17341 acggtggata attcccggcg tatcgtaaat ataagatccg tcgtcaagcg gaatctctat
    17401 tttgtccaag gttgtccctg ggaagcgtga agtagtgatg acattctgat cacccgtgat
    17461 ttcttggata atagcattga ttagagttga ttttccaacg ttggtcacac cgaccacata
    17521 gacatcgcgg cccttacggt agtgttcaat cttgtcaatg acttccttaa tggcatgttt
    17581 attttgtgct gaagttagga ccacatcgac tggacgaaga ccttcttcat gggcacgttt
    17641 catgagccac tggctaatct taccagactt aactgactta ggaaggatat cttttttatt
    17701 tcctaccaag aggacatcat tgcccgagac gaaacgtggt aaacctggga tgacagatcc
    17761 attaaaatca aagatatcaa tgacattgac cactaaagca tcactgtctc ccacctcgtg
    17821 caagagcttg aggaaatcat cgttcgtcaa ctggacatct gtgatttcat tgtagtggcg
    17881 gagacggaaa cagcgttggc aatagacttc gccagtctcc aaaccttttt caagtgccga
    17941 ctggggggta aaaccaagac cagctttatc tgtcgtctga atggttgctc cacaaccaat
    18001 acagagaatt tcttccatag ttaaattcct tttttatatg taatcggtcc gtacttttca
    18061 gtgatttttc tcataacacg acgctcacga gttcggttaa tctgcgtttt gattgagtca
    18121 tgttggacca agggtttgac taaaattgac cgaatccctg cacggtgggc tgctcgtata
    18181 tctgtcatga gctggtcacc aaccatgacc acttcctttt tgtcatagtg gaattccttc
    18241 atagcacggt caataccaaa tgtgaagggc ttcaaggccc agtaaacgta atcaatccca
    18301 aatttctcaa ctgctcgttg aacgcgtttt ttggtgttat ttgacactac gataatgcca
    18361 atacccgcgt cccgaaggtc atgtagccat tgcttcatct ctggcgttcc atcagggttg
    18421 ttccaagcaa tgagggtatt atccaaatcg accaaaacag ccttgattcc ctgcgcctgc
    18481 aggctgggga ctgtcagatc atagactgct tccacagcaa aatctggtat ataattttca
    18541 atcgccattc tggctccttt tcttaacttt ttttcgcaat tttccttagt aacaaggata
    18601 aaacaatcca caaacctgca ctagccaaac tgatatagag ccaagcatgg ggctcatctg
    18661 ttaaaggtaa gggaacattc attccgaaaa agcctgtcac gactgccaaa acagctagca
    18721 agactgaaat gatagtcaag gttgtcaaat tgtcattcag attattgttt agaatattgt
    18781 tgtaagaggc tgaaagctgc tgtaaaatct gagagattag gtctgtcatg gataccagct
    18841 gatgagcctc aatcatggca tcatcaaact gttctctctc aatctcatca aaactacgat
    18901 acaaggcatg accttgaata tgctctaaca aaatccgatt ttgtttggca gctgccgtca
    18961 gataaaccat accagtctcc aaatcagaca gggcaaagag gtttttcttg gtagttcgct
    19021 ggcgcaagag gtcattgacc tcatccctac tcttgtccat ctgctcaatg acaggatagt
    19081 aggcattgct gatgatttcc agactggcaa agagaaactt ataaatcgaa agcgtgtcat
    19141 ggctctccag ataacgagtc atctgttcaa tgacataggc gttcttggta ttactaatgg
    19201 taatcaggcg acgatgctcg acaataaagg tcatgggaaa agtctcatag taggccttgt
    19261 ccttttttac gtctaggaca ttatagataa aggtaaccgt cccactttca cggtggtagt
    19321 ccatgtgggc gcgctcgttt ctatccagtg cgtattcaat ggtttccttg tccaaaccgt
    19381 aaatttcaga aaggtcttct agtttattca actttcctag gtctaggtct atccaggtac
    19441 aaccattgcc caactgcttt tctaaaacca tcttttctcc ttattaaact ccctctattg
    19501 taccacaaat tacttaaaat aacaggtcta gtctgtgcgt gagaaattgc taacaactgt
    19561 gatatttcgg ttagggacaa agtgaagggc ttgctcctcg ctacgaagtt gtgtcgtacg
    19621 aattcccaca ctgacaacct tacccgatac agtaatcggt ccatttgtca gaacgacctc
    19681 atctcccaca tccagttgac gttcaaagag gatgaaaaag ccattgatga catcagacag
    19741 aaagccttgg gctcccatac caatcgctac cccagcaata ccagctccag ccagcaaact
    19801 agaaactggc aaacctaaaa tcgacaaaat gcagtagagt aaaaagaaat atagcgtata
    19861 attaaacaca ttttctagta aacgtgagat ggttttttgg cgtccgacat catgacgaga
    19921 catttttaga gaaggtttga caattctctg caccatggta tgaagcattt ttttagcaat
    19981 ataaaataca atcaaaagca gtaaaagaga aatgaccttg gttagaatat tctcgataat
    20041 ggttgtcaca tctagctttt caatataagc ctgaataaat ttttgcatat aaaactcctt
    20101 tatttcatta taccatattt tcatcagtct cattctccat aaatattcaa aaaatagaca
    20161 gaaaattctt gattttgtct atcatttata ctataatcat aatcactaca caaaaaggag
    20221 attattatga aaagaatcat tcatgcttgg aataaggcaa gccttgtcaa gcgtatcttg
    20281 attggtatgc tttgtgggga atcctaggac tgacccttcc taatctctca gggattgggc
    20341 tactcgggga tttattcgtt ggaggtctaa aagctgttgc tcctatccta gtctttgccc
    20401 tcgttgccaa tgccctttcc caacatcaaa agggacaaga tagcaatatg aaaactgttg
    20461 ttttcttgta tatcctatga tagggacttt cgccgctgct cttgtagctg tactagcaag
    20521 tttcatcgtc cctattgaaa ttaccctaaa tagtgccaat actgaaattg caccaccaga
    20581 tgggattggg caggttctca gcaacctctt gctcaaactg gttgacaacc cagtcaacgc
    20641 cctgcttact gctaactata ttagaatctt atcttgggca gtcatttttg gaatcgctat
    20701 gagagaagcc agtaaaaata gtaaagaatt gctaaaaact atcgctgacg tgacttctaa
    20761 aattgtcgaa tggatcatca atctggctcc atttggaatc cttggtcttg tttttaaaac
    20821 catttctgac aagggagtcg gaagccttgc caactacggt attttattgg ttctattagt
    20881 aacgactatg ctttttgttg cccctgtggt caaccctttg attgccttct tctttatgag
    20941 acgcaatcct taccctctag tttggaactg cctccgtgtc agcggtgtga cagccttttt
    21001 cactcgtagt tctacgacta acattcctgt caacatgaaa ctctgccatg accttggact
    21061 caacccagat acctattctg tttctatccc actcggttct actatcaata tggctggagt
    21121 agcgattacc attaaccttt tgacccttgt tacagttaac actcttggaa ttcctgttga
    21181 ctttgccaca gcctttgtcc tcagtgtggt agcagctatc tcagcctgtg gtgcttcagg
    21241 tattgccgga ggttccctcc ttcttatccc agttgcttgt agccttttcg gtatttctaa
    21301 cgatattgcc atacaaattg ttggggttgg ttttgtgatt ggtgtcatcc aagactcatg
    21361 tgaaacagcc cttaactctt ctacagatgt cctctttacc gccgttgccg aatacgcagc
    21421 aacccgtaaa aaagcaagcc tgcttatgtc ttgtctttta cgcttttatt ctaacttatt
    21481 aggaaattct tatgtctatt agccaacgta cgaccaagct catcttagct acctgtcttg
    21541 cctgcctgct tgcttatttt ctcaatcttt catcagcagt ttcggctgga attatcgctc
    21601 tcttgagcct atctgatacg cgtagaagta ctttaaaact ggctcgcaat cgtctttttt
    21661 ctatgcttct agctctggct atcggtgttc tagcttttca cttgagcgga tttcatatct
    21721 ggagtctcgg cctctatctg gccttctacg ttcctttagc ctacaagatg ggctgggaaa
    21781 ttggcatcac accaagcact gttttggtta gccatctctt ggttcaagag tcaacctctc
    21841 cagaccttct agtcaatgaa ttccttctct ttgctattgg tacaggattt gccttgcttg
    21901 ttaatctcta tatgccttca cgagaagagg aaatccagca ctaccacacg ctggtggaag
    21961 aaaagttaaa agatatcctc cagcgcttca aatactattt atccagagga gacggacgca
    22021 accgagcaca gctggtagca gaattagaca cgcttttgaa agaagccctc agactggtct
    22081 atttggatca ctctgaccac ctctttcacc agaccgacta ccatatccac tactttgaga
    22141 tgagacaacg acaaagtcgt atcctgagaa acatggccca acagattaac acttgtcacc
    22201 ttgccgccag tgaaagcctg atcttagcgc aactcttttc aaaaattgca ggtcaactga
    22261 gccagaccaa tcctgcttct gatttgctag atgaaattga acgttatctg gaagtcttcc
    22321 ggaaccgcag tctgcccaag acaagagaag agtttgaaac ccgcgccact cttcttcaac
    22381 tcctacgtga agccaaaacc ttcatccaag taaaagttga tttttaccaa aagtatggac
    22441 agtaaaaaag aaaaccacta aaaaacagag cttgccagca ttcttgaaaa gtagtataat
    22501 tattgcatgc gcaatcatct tgtgacgcag agactgcccg ttaatggact ttcttctatt
    22561 tatgactcta aaaagaaagg agacactatg acctatttgg aaaaatggtt tgacttcaat
    22621 cggcgtcaga aagaaattga aagtctcttg gaagagacca ttgcccaaca gagcgagcaa
    22681 agtctgacct tgaaagagtt ctacctgctc tactatctgg acttggctga agaaaaatct
    22741 ctacgccaga ttgacctgcc agataagctt catctgagcc cgagcgcagt ttctaggatg
    22801 gtggctcgct tggaagctaa aaattgcggt ctactcagtc gcatgtgttg ccatcaagat
    22861 agacgctcta gttttatctg cctgacgaat gatggacaaa agacactagc ctcgctacaa
    22921 aagactgtcg aagaaagctt ggaagcaggt ttggatttct tgatttaaga tgattttaga
    22981 aaaagttgcg tgcgcaatca tttttcttga cattctcttt tataaggagt aaaataaagt
    23041 tagcattaaa caaaggagtt ttaaatatga ttgaaattac ctatatagat gccagcaaga
    23101 acgaaagaac tgtgactttc gagtcttatg aagactttga acgttcgcaa caagcttgcc
    23161 ttatcggcgt cgcagactac taccctgtcc aaaaattaac ttacaagggt cataatttgg
    23221 actaccatgg gacttatgga gatatcttct tctatctcat gaaacaagat ttaagccaat
    23281 ataactaaaa aaaggagaaa tacaatggca aaagcaatta cagatgcaac attcgaacaa
    23341 gaaacaaaag atggtttggt cttagtagac ttctgggcaa cttggtgtgg tccatgtcgt
    23401 atgcaaggtc caatcttgga caaattgtct gaagaacttt cagaagatgt cttgaaaatc
    23461 gttaaaatgg acgttgatga aaatccaaac acagctcgtg cttttggaat catgtctatt
    23521 ccaactcttc tcttcaaaaa agacggccaa gttgtcaaac aagttgcagg tgttcacaca
    23581 gcagaacaaa tcaaggccat cattgctgaa ttgagctaat caaacgagag accaagtact
    23641 ttctttggtc tcttttttct tgccctttgc catttttcaa aaaatatgct agactatagt
    23701 agattgaaac tagaatagta cacctctact tct
//
LOCUS       DAVKAO010000026        23432 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKAO010000026 DAVKAO010000000
VERSION     DAVKAO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.026"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            211..351
                     /locus_tag="VWX16_001068"
     CDS             211..351
                     /locus_tag="VWX16_001068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158439.1"
                     /translation="MDRTDEVSSKHCFEVVDRTDEVSSKHRFEVADRTDEVSNIYTAR
                     QS"
     gene            complement(705..1685)
                     /locus_tag="VWX16_001069"
     CDS             complement(705..1685)
                     /locus_tag="VWX16_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9158440.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(2001..4016)
                     /gene="recG"
                     /locus_tag="VWX16_001070"
     CDS             complement(2001..4016)
                     /gene="recG"
                     /locus_tag="VWX16_001070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9158441.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKTEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(4035..5138)
                     /locus_tag="VWX16_001071"
     CDS             complement(4035..5138)
                     /locus_tag="VWX16_001071"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9158442.1"
                     /translation="MKASPHRPTKVLIHLGAIRQNIQQMGAHIPQGTLKWAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLNLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(5128..5496)
                     /locus_tag="VWX16_001072"
     CDS             complement(5128..5496)
                     /locus_tag="VWX16_001072"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9158443.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5535..6566)
                     /locus_tag="VWX16_001073"
     CDS             complement(5535..6566)
                     /locus_tag="VWX16_001073"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9158444.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEVFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6568..7599)
                     /locus_tag="VWX16_001074"
     CDS             complement(6568..7599)
                     /locus_tag="VWX16_001074"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9158445.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7680..10193)
                     /gene="secA"
                     /locus_tag="VWX16_001075"
     CDS             complement(7680..10193)
                     /gene="secA"
                     /locus_tag="VWX16_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9158446.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMLEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(10339..10626)
                     /locus_tag="VWX16_001076"
     CDS             complement(10339..10626)
                     /locus_tag="VWX16_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158447.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10637..11287)
                     /locus_tag="VWX16_001077"
     CDS             complement(10637..11287)
                     /locus_tag="VWX16_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9158448.1"
                     /translation="MKLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(11291..11932)
                     /locus_tag="VWX16_001078"
     CDS             complement(11291..11932)
                     /locus_tag="VWX16_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158449.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEVNLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11925..12716)
                     /locus_tag="VWX16_001079"
     CDS             complement(11925..12716)
                     /locus_tag="VWX16_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158450.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIVTYFFGTFSPLGWNSLFSDGSSLQR
                     LLDGEITSYLFFTCVLLIGIFINAVYFFKIVDYLGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12901..13236)
                     /locus_tag="VWX16_001080"
     CDS             complement(12901..13236)
                     /locus_tag="VWX16_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158451.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     SLFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(13376..13711)
                     /locus_tag="VWX16_001081"
     CDS             complement(13376..13711)
                     /locus_tag="VWX16_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158452.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13802..15112)
                     /gene="der"
                     /locus_tag="VWX16_001082"
     CDS             complement(13802..15112)
                     /gene="der"
                     /locus_tag="VWX16_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9158453.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRR"
     gene            complement(15126..15839)
                     /locus_tag="VWX16_001083"
     CDS             complement(15126..15839)
                     /locus_tag="VWX16_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9158454.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15836..16732)
                     /gene="dnaI"
                     /locus_tag="VWX16_001084"
     CDS             complement(15836..16732)
                     /gene="dnaI"
                     /locus_tag="VWX16_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9158455.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKFSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKGKNGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16733..17902)
                     /locus_tag="VWX16_001085"
     CDS             complement(16733..17902)
                     /locus_tag="VWX16_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9158456.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17903..18376)
                     /gene="nrdR"
                     /locus_tag="VWX16_001086"
     CDS             complement(17903..18376)
                     /gene="nrdR"
                     /locus_tag="VWX16_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9158457.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            18516..18881
                     /locus_tag="VWX16_001087"
     CDS             18516..18881
                     /locus_tag="VWX16_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9158458.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18886..19581
                     /locus_tag="VWX16_001088"
     CDS             18886..19581
                     /locus_tag="VWX16_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158459.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19598..20365
                     /locus_tag="VWX16_001089"
     CDS             19598..20365
                     /locus_tag="VWX16_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158460.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20718..21917)
                     /locus_tag="VWX16_001090"
     CDS             complement(20718..21917)
                     /locus_tag="VWX16_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158461.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMTKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21914..22807)
                     /locus_tag="VWX16_001091"
     CDS             complement(21914..22807)
                     /locus_tag="VWX16_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158462.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22923..23003
                     /locus_tag="VWX16_001092"
                     /pseudo
     CDS             22923..23003
                     /locus_tag="VWX16_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         6927 a         5224 c         4359 g         6922 t
ORIGIN      
        1 tttcaaaaaa ttcttttata taataatgtc cttgaaagta gtttcagctg aacatttata
       61 gtccataaaa agaaaacaga aactcatctg tttttatact caatgaaaat caaagagcaa
      121 actaagaagc tagccgcatg atgctcaaaa cactgttttg aggttgtaga taagactgac
      181 aaagtcagct caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagctcaaaa
      241 cactgttttg aggttgtgga tagaactgac gaagtcagct caaaacaccg ttttgaggtg
      301 gcagatagaa ctgacgaagt cagtaacata tatacggcaa ggcaaagctg acgttgtttg
      361 aagagatttt tgaaaagtgt taacacctac agacaagcct gacgatagca agaactaccc
      421 tactcgatag gtatcagctt ttgctttctg aaaaaaatta ttttaagcaa tttgacaaat
      481 ctagcaacaa aaaattctat aaatataata gattgaaact agaatagtac acatctactt
      541 ctaaaacatt gttagaaatc gatttgactg tcctgatcga tttgtcctgt tcttgtttca
      601 ttttactata tttccatgat aaaacgcata gtatcaagtt tttttaatcc cctgatacta
      661 tgcgtgtttg taatttttaa gattttgtgc ttagagtcga ctccttattt tagatattta
      721 aaaggaatct cacttccaca gagccagttg tagacttggt cattgacaaa tacattcatg
      781 gcttcgtgag catactcagg catgatgcga taggttttat cgcaggtcag acgattataa
      841 atcgcaaact gggtaatggg atagcaaaca tcgtcgtcca agcccgtaat catcttaacc
      901 tcaccttgga tacgatgggc aagatttttg acatcgatat aggcaagggt cgccatgatt
      961 tcctcctctg tttcatggaa ggggtcgtga aacttgaaat aacggaaaag ttcgtcgtaa
     1021 gcctcgctag tattaccaat ctcaatcacc cgtctgaagt ctgacaagaa gggataaatg
     1081 gcaactgttt tctgaattcg aggattgagc gctgctgcaa ctagagctag agcccctcct
     1141 tgtgaggcac catagctaga aagacgcttc tcatcaacct gagacagact agcaacaatt
     1201 tcgaccaact ggtaaatatc cagataaaca tccttataaa agaggtggtc ccgaccttcc
     1261 acagcaccac ggataatatg ccccttcacg gtatttccta aaggagaacg caagccgtct
     1321 tgtgagtaac ctgactgacc ccgcacatcc atggaaacaa caccgtaacc agctacggtg
     1381 aagcccagca tgtcggccca gtcccagcca cgtcccatat aaccatgaaa atggaagatt
     1441 agtggaacct tctcctcact ctttggaaga acaatgcgtg catagacctt tccttccttg
     1501 cttccttcaa atgttaactc atagcacttg acttgaggaa tgtggaaatc tctttcctcc
     1561 aagtggtagg atggaagcgt ggaaacattt ttcacttccc catcccagaa agcatcaaag
     1621 tcttccggaa cctcatccct tcctctatag gtcttaattt cttctagcaa agctggattt
     1681 ttcatagcat gctcctttta ttttgtaatc gttaccacaa ctgtagcata tctatgtaat
     1741 caatgcaaga aagaaggata aatagaaagt gaatttagat tctttacttt taggatgatg
     1801 ggctaaaagt agttttagaa acgaaaaaga gcatttcgct ctctcttctg tttatactag
     1861 ttttttgctt ccttcttttg gaaagtggtt tggtaattta ctttaatcgt tactgtcata
     1921 tgagagtcct cgtttctttt tgatgacttt agtataagag ccaaacctaa aaactagctt
     1981 aagttttcct tagagaaagc ttaatccaga tgttctttct tttccagatg aagggcaatc
     2041 atgcgccact ctggatcttc ttgccaagct tctatagaac taatgtagct agcaaccttt
     2101 cttgcttctt ctaaaatcgg aaaatcttcg ataatatcag ccacttggaa ctctggaagt
     2161 cctgactgtc tggttccaaa aatctcacca gaaccacgca ttttcaaatc ttcctccgca
     2221 aggacaaatc cattggtcgt ttctgtcatg atacgcatgc ggtctttccc agaatccgtc
     2281 ttgggattgg caacgagaac agcgtaggac tgcttgtccc cccgaccgac acgacctcta
     2341 agctggtgaa gttgactgag accgaagcga tcggcatcca tgataatcat gacagtcgca
     2401 ttgggaacgt tgaccccaac ctcaataacc gtcgtcgaaa ccagaatatc cgtctttctc
     2461 tccttgaaat cctgcatgat ctggtctttt tcgtcactct tcatcctacc atgtagaaga
     2521 gccacctctg tcttgcctgc aaaatgagtc gtcaactcct ctgataaggc aatggcattt
     2581 ttcaaatcta gagcttctga ttcttcaatc aaaggagaga tgacatagac ttgggaacct
     2641 ttttgaattt ccccctctaa ccaagtcaag acctgaggta gttgctcatg tttgatccag
     2701 cgcgtcacaa taggcttccg acctgctggc atctggtcga taatggaaac atccatatct
     2761 ccaaaggctg tgatggcaag cgtccgtgga atgggagtcg ccgtcatcat gaggacatct
     2821 ggattgtcgc ctttttcccg taaaatacgc ctttgcccta caccaaaacg gtgctgctca
     2881 tcgataataa tcaaaccaag acgagcatac tccaccccat cttgtataag agcgtgagtt
     2941 cctataatca aatcagcctc acccttggca atggtctcca agacttctct cttttctgca
     3001 gctttcaagg aacctgtcaa gagagccagt ttcaaattgg gaaaaaggtt ctgtaaactc
     3061 tcaaagtgtt gctctgcgag gatttctgtt ggtaccatta gggcagcctg ataacctgct
     3121 gtcactgccg caaacatggc caagccagcg actaccgttt ttccgctccc cacatctcct
     3181 tgtaggagac gattcatgtg gtggtcggac ttcatatcag ttaaaatttc ctgcaaactc
     3241 ttttcctgag cttgggtcag ggcaaaagga agacttgctt taactgctgt cactttttcc
     3301 tgagaccaat ccagaaccag accacttccc tgaactctat tttcagactt gagcgtctgc
     3361 agctgcattt ggaaataaaa gagttcctca aacttgatac ggcgaagagc ctgcttgtat
     3421 tctgccaaat cctttggaaa atgcatagct cggactgcct gacaacggga catgagtttg
     3481 tatttgtcta gtaaagactg gggcagattt tcttctatca agaggtccag tccctgatca
     3541 aaagccgtct tgatgacctt gaccagactg gcctgactga ttccctgagc cagacgatag
     3601 acaggctgga ggtcatcttc tacctgagcc agaaccttca tcccagtcag actagcctta
     3661 gcgcggtccc attttccaaa gacagcaagg gttgctccca actctatttt atcagccaga
     3721 tagggctggt taaagaaatt caccgcaaaa acgacctctc cctgcttgag actaaaacgc
     3781 aggcgattgc gcttgaaacc ataatactgg acactagcag gagtcactac ctgaccagaa
     3841 agaactgcct tctcaccgtc ttctagttcc agcacctgct tggttttgaa gtcttcataa
     3901 cggaaaggaa agtagagcaa gagatcttgc aagttttcaa ttcctagttt ggcgtatttt
     3961 tctgctgact ttggtcccac accaggcaag acatgcaagg gttgatgtag attcatgctc
     4021 cactcctttc ttttctaata atattctctc ggaatacggt cgctgaggag gcaaaccacc
     4081 tcatagttaa tggttacgcg gtaggtcgct acctgagttg cagtgatttc cttatcccca
     4141 ttggagccaa tcaaggttac cttggttcct agcggataaa gcttaggcaa tcgaatagtg
     4201 atttggtcca tcgaaaccct gccgacaatt gggcaagctt ggccatctac caagacagag
     4261 aaattttgca tgtctcttgt ccatccatct gcatacccga ttggcacggt cgcgatgact
     4321 tgctcgctat ccgcttgata agttgctcca tagcccatgc aagctccagc tggaactgtc
     4381 ttgacatgaa ccagagcaga ctccaaggtc aaggccggta tcaaatcata aggcaaattc
     4441 aagaccgctc cacttggatt gaggccatac atggcatctc ccatacgaac cgcattgaaa
     4501 atagtctcta catgccaaag agtcgttgca gaattgctag catgaaccag ctctggaact
     4561 tccttcatac tagctaaaat agttttaaac cgttctaact gggcattaaa atagtcatct
     4621 gattcctcat cagcagtagc aaagtgggta aagattcctt caacacgaac accgtgttgt
     4681 tggagcaaat cttgagcctg ctcaacctca ctggcctctc taaaaccaat ccgtcccatc
     4741 cctgaatcaa tcttgaggtg gactgtcaat ccagttaggt ccacttcctt atctaagagt
     4801 gcttgaatcc actccagtcc agccactgtc aaggtgaagt catattcttt agctagagca
     4861 acagcttcga tttcagaaac tcctaaaatg aggattggct tgctgagtcc agcttgtctg
     4921 agttcaatgg cttcatcgat attggaaacg caaaagccat caacatcatc ttgaattgcc
     4981 ttggcaacgg caacagctcc atggccataa gcattggcct tgaccacagc ccacttgagc
     5041 gttccttgag ggatatgagc ccccatttgc tgaatatttt gtcgaatagc tcccagatga
     5101 atcagaacct tggttggtct atgtggacta gctttcatga ttttcctcca aaatgacact
     5161 ggctgtcaca aactgatcgg tgtggctgat agacagccaa atctttcctg aaaatggtgc
     5221 ctgactaaaa taaggcgccc cacgttcatt gttcaatact tccaaatcct gaaaaccgag
     5281 cttgctaatg cccgttccca tagccttgga aaaggcctcc ttagccgacc agcgaccagc
     5341 taaatattct atttgcctgc gtcctttgag actggtgaag cgctccattt cctgagcggt
     5401 cagtacacgc ttagcaaatc cttcatgtcg tgtaactgcg ctttctatcg aagccaattc
     5461 ttcgatgtca attccgtgtc caactatcat tctcatcaaa aggagttgag attccccaac
     5521 tccatccttt tcacttattt tgtcaaggta acataaatct cttctaccaa ggcctctgta
     5581 ttttcccaac ctaggcaagg gtcagtaata gagcaaccaa agacctctgg ttggttttga
     5641 cgaccatctg ctaggtaaga ttcaatcata aatcctcgaa ccgtcttttt aattttctca
     5701 ttccaatcac gattctgcaa ggtctggcga acaattcgaa tctgctccat atattgcttg
     5761 cctgagttat catggttggt gtcaatgagg ataaaaggat tttcaagtcc catggtttca
     5821 tagcgttcaa tggcttggag taggttttca tagtagtaat tcggcatata attgccatac
     5881 tcgttgactg ctccacggag gataacatgg gccaaaggat tacctgatgt ctcaacttcc
     5941 tgcccatgat aaaggaaggt ttgcttgttt tgagcagcat agatggcgtt aaacataaca
     6001 cccaaatttc ctgaggttgg atttttcatc cctactggtg catcaatccc agaagccaca
     6061 aagcggtgct cttggtcttc cacagaacga gctccaacgg catggtagct gaccaagtca
     6121 tccaccaaga tcagatttga cggataaagc atctcatctg ccgttgtcaa accagtctct
     6181 gtaatcacgc ggtagtgcaa ctggcgcaca gcctgcaagc cgttaatcag gcttggagcc
     6241 ttagaagtat ctggctggtg aaccaaccct ttatagccgt ctccattggt acgaggctta
     6301 gcagtataca cgcgcatgac catgaaaatc ttatccgcta ccttcttttg caaggcggat
     6361 aaacggcgag catattccaa gaccgcctct tcattatcag aagagcaagg accaatcacc
     6421 aagagaatac ggtcatcttc ccctgaaata atatctgcca attccctgtc acggctttcc
     6481 ttgagtctca aggcttctgc agacaattgg gtttcagcct tgatgacttc catatcgatt
     6541 tcttgacctt tttcaataaa tgccatctta ttctcctaac gttttgtaaa tttctctgac
     6601 aagagcttct gtgttatccc aacccaggca agggtctgtg atagacttac caaatacttc
     6661 tggctcattt tgtcgaccat cttccagata agactcaatc ataaaaccac gaacgaactg
     6721 cttaattttt tcattccaag cacggttaat caaggtctgg cggacaattc ggatctgttc
     6781 aatatactgc ttaccagaat tgtcatgatt ggtatcaatg atgataaaag gattttccaa
     6841 gcccattttc tcatactggg caatggtatc aattaaattg tcataatagt agttgggaat
     6901 attttttcca tactcattaa gagcaccacg aagaatagcg tgtgaaagcg ggttcccagt
     6961 tgtttctact tcttttccta agaaaaggaa actttgtttg ttttgagcag cataaatccc
     7021 attaaacatg acattgagat ttccagaggt tggattttta aaaccagtag aaaatcctgc
     7081 cccacttgcc acaaagcggt gttgctggtc ttcaactgaa cgggcaccaa ctgccatgta
     7141 agaaatcaaa tcatctacaa gcggaaggtt ttcaggataa agcatttcat cagcagttgt
     7201 catccctgtt tctgtgatga cacgatagtg aagatggcga acggctttga ttccattgat
     7261 aagactaggc gcttctgtcg cgttaggctg gtgaatcaag cccttatagc catctccgtt
     7321 ggtacggggt ttggcagtat aaacacgcat aaccataaag atacgatctg ccacttcttc
     7381 ttgtaggact gccaaacgct tagcgtattc aaggacagct tcttcgttgt cagatgagca
     7441 tggcccgatt accaagagaa ttcgctggtc ttctccacgt ataatggctt ctagctcttg
     7501 atctcgctgt gatttcctct ccaaagcctg accttctaat tttgacaagg cacgaacttc
     7561 ttctatattt attttagagc tttttgctgt aaataccata actcatctcc ttataaagca
     7621 aacggatacc cagtaaaaat ttacttttca caagatatcc gcctctaaac tatctcattt
     7681 tattgtcttt taccgtgaca gtttttaaat ttcttaccag aaccacatgg gcaaagttca
     7741 ttgcgtccaa tctggctcaa atccaaatct tctagcatac ttgcttggtg agcagcgata
     7801 ttgcgagtcg ctgttgtact gatatgacgt tctgcctgtg gtctttcttg ttcatgaatt
     7861 tgtgctttca tcatcaagcg tgtcacatca aactcaatcg aaccaatcat atcattaaac
     7921 atacggaaac cttctgcctg atactcaaca acagggttgt tctgagcata gccacgaagt
     7981 ccaaccgcgt tacgcaattg atcaagggca tcgatatgat ctgtccactt gttatccacc
     8041 actcgtagaa tcaaaacttt ttggaattct ttaactgctt cttcatcgcg tagttttgaa
     8101 acctgactat cgtaaacctt caaggcacgt tggaaaagct cttccttgat ggccttatca
     8161 gacaagcctg acaagtcttc catcgtaata gaatcttcag gaagcaagtt gtacttagca
     8221 aagttcaaaa ttgcctctag tttttcatct tgtttggcac gcgcatgacc atcaacgaca
     8281 cgttcaatcg tgcgcttaat catagactga atttcaggtg ccaagtcacg atctgcagtg
     8341 atgacatcgt aacgttgagc atagataatc tcacgttgtt cacgcatgac atcatcgtat
     8401 tgaaggactt gtttacgggt atcgtagtta tttccttcga cacgtttctg agctgcttca
     8461 acctgacgcg tcaacatgcg agactcaatg gcctcttcag acatgttcaa gcgttcaaag
     8521 attcccttca agcgttcaga accaaaacgt ttcatcaaat catcttcaag agataggtag
     8581 aattgtgact cacctggatc tccttgacga cctgaacgtc cacgaagctg gttatcgata
     8641 cgacgacttt catgacgttc tgtaccaata acacaaagtc ctccaagttc acgaacacct
     8701 tcaccaagct tgatgtcggt accacgaccc gccatgttgg ttgcgatggt aacggcacca
     8761 cgttgaccag cattcatgat gatttgggct tctctatagt ggtttttggc attcaagact
     8821 tcgtgaggaa caccagctgc aaccaatttc ttagaaatgt agtcactagt ttcaaccgct
     8881 actgtaccaa ccaagacagg ttgacccttt tggtaacgag ccttaacgtc ttcgacaacc
     8941 gctttaaact tagattcgat acttgcataa agaaggtctg agtggtcaat acgttgaaca
     9001 ggacggtttg ttgggattgg aataacacga atgttgtaga tttcacgaaa ttcttcttcc
     9061 tcagtcttac ctgtacccgt cataccagac aattttttgt acatacggaa aaggttttgg
     9121 tacgtgattg aggcagatgt cttggtttca tcctggattg gcacaccttc tttggcttca
     9181 atagcttggt gcaatccatc agaataacga cgaccttcca tggtacgacc tgtaaattgg
     9241 tcgacaatca agatttcttg ctcttcgctc accacatagt caatatcgag aagcatgatg
     9301 tagttggcac gaagggcgtt atcgataaag tgggtcaaag ccacgttttc gatgtcatag
     9361 aggttttcaa gtttgaagta gctttcagcc ctgtcaatcc ctgaatcaga caaaccaata
     9421 gtcttagact gcacatcgat gatgtagtca tctttgttca aagattttac atagtggtct
     9481 gccatgtgat acaactgact ggtttcaacc gcattggcac ctgatacaat caaaggtgta
     9541 cgagcctcgt caatcaagat agagtcaacc tcatcgacca aggcatagtt aagcggacgt
     9601 tgtaccatgt tttcagcgcg aacgaccata ttgtcacgaa ggtagtcaaa tccgatttct
     9661 gagttagttg agtaagtaat atcacactca taggcttctt ttttctccat tggagatttg
     9721 gtagccaagt taatccctac tgacaaacca agccaagagt acaattcacc catctcagtc
     9781 gcgtcacgtt ctgacaggta ttcattaacc gtaactacgt gaaccccttt acctgaaagg
     9841 gcattgaggt ataccggcat ggtcgcagtc aaggttttcc cttcccctgt acgcatctct
     9901 ggcacgtcac catggtgaag aacaatcccc cccatgacct gaaccttata agggaagaga
     9961 cctaggacac gtttggcacc ttcacggaca accgcaaatg cttcgtaaag caatgaatcc
    10021 agtgattctc cattttgata acgttcctta aattcaactg tttttgcttt tagttggtcg
    10081 tcagtcaaag cagccatttg gtcttcgtat ttgaaaacct tgtcagccat cttttccaga
    10141 cgacggattt ctcctttatc attttcgata attgttttta aaatattagc catgtttttc
    10201 cttactttaa attccgaata ttttagaatg ttcctttaat tttagcacaa ttactgatta
    10261 ttttcaagga agaatcccca ttttgaaaag gaaaaagagg ttagttttca agctaacctc
    10321 tccgactttt atgactgttt aattgccaaa aattggcaaa atggcaaatg tgataaataa
    10381 gttaatccaa agagaaagac agcaaatcag tccaaaaaca aagagactga ctttcttgga
    10441 cagcaaggcc atcaaaatgg acagaattcc aaaaactagc aagactttct gtatgatgaa
    10501 gatgtcaatc cttaccccca gaatcgctct atcccaaggg ataaagaaga aagacagcca
    10561 gatcagcaga actaaaccaa tataaacctt ggaatagcgt gtaataaatg attttagatg
    10621 tgccataagc catctcctag gattgtatat cctttacata ttgtatatgt ccattttcga
    10681 caactaatac atctgtacaa aggtctgaaa tatcttccga tatgtgcgac gtcaggataa
    10741 ccagaccatt ttcttttttc aggtaagata aaatgacctg tttggttaac ctcacactct
    10801 tctcatccaa agcattcata ggttcatcga gaaagagtat agaaggagag gaaataaagg
    10861 cttgaatcaa ggccattttt tgctttgttc ccaaggataa atgccggtat tcaacgtctt
    10921 caaattcctg taaatcatag tattgaatcc aataggcaat tcttttttcc gtaactttag
    10981 atgaaaaata ccttaacagt tccaaatttt ctctcaggga taaatgagat aaaaactcga
    11041 ctttttcaat taaaattcct gcatcctgaa tataatgatt ttttatcccg taaactttac
    11101 catcttgaag aacttttcct ttgtcaagct taatataacc agcaagtacc tttaaaagaa
    11161 ccgtcttgcc agatccatta tcacctttaa gtccataaat ttttcctgat tgaaacacca
    11221 aattcaactg atttaaaata ggtcttttcc cataaatttt ctgtccattt ataatttcaa
    11281 gtttcatcgt ttatcctttc ttaaatttct cttccaagcg actaacaatc aacataacta
    11341 ataagcttaa gagtagtaaa tacactgtta caatatgatc catcatccat tcttgtccca
    11401 caaatgctaa aaaatagaga aaaaaggaaa aaccagcgcc cgcactaatc agtgctatca
    11461 gataagacag tagtaagtac aacatcatta actgagcaag taaaaagaaa agtgcgactg
    11521 ttttgaaatg aattcctagc ggaagtaaac caaggaacca gacaagtaaa tcttgagtaa
    11581 aaaatttaga aatgacaacc aatcgtttcc ttgtataatc gaaaaaggag cttgattgat
    11641 agcgaatcaa gtgatggtaa tggtctgcca aatcactttc aagtaaaatg acagagagcc
    11701 atcctaccat gaaaagcgaa aggtttaaca agagtttggg gatttctaaa ttcacttcag
    11761 aatagtcatt ataggaaaaa attgaaatca attctctctt gaaaaagata acagcatcct
    11821 gcttttgaac cgctgttgac aatagatggt ttatcaaaat catcatcaga actatacgaa
    11881 gtagtaaaac aatctttctc tttagcaaaa tattgttaaa attcttaaac attatcttca
    11941 tatttttcaa gcaccatgta agggacaata taaaggatat aaggagtaaa gagtttcatc
    12001 aaacttacat ccccatagct agcttgcatc aaactcgtca taggaaccat atagtaaggc
    12061 aaggcactat agagcagcat agaaccaagc caaagaaaca tcaaataggt gattgccgaa
    12121 cgagtcacat ttcctaaata atcaactatt ttaaaaaaat agactgcatt gatgaaaata
    12181 ccgattagta ggacacaagt aaagaacaaa tagcttgtta tctctccatc taggagcctt
    12241 tgtaaactac tcccatcaga aaatagagaa ttccatccaa gaggagaaaa agtcccaaag
    12301 aaataggtta caattgcaat tatcagcaca gtcactaaat atatcaaaca agggatactt
    12361 gcaacttgca aagtcaactt tctttttaac ccttgataac tcacttctct tccaatactc
    12421 aatcgtaaaa ctttattttt cagctcaata tattgaaaac ctagcaaaac aatgatgatt
    12481 ggaataataa atgattgata aaaacctaga ttgaactgga gaatacgaaa atctaatgct
    12541 gattttaata agtcattttt aattgcttgt ccatcgaatt tagctagatc gggcgcatga
    12601 atatcaaaaa agaaataagt catcaaaaca tcacgataaa ccaaagcaag aaaaagcaaa
    12661 gtaagcaaga cataaacaaa tttatgcctt gccaaagtta agagaggaat cttcataagc
    12721 cacctcctac aaatagaaag taatataata ctcaatgaaa atcaaagagc aaactaggaa
    12781 actagccgca agttgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag
    12841 ttacatatat aatccaaggc gacgttgacg tggtttgaag agattttcga agagtataaa
    12901 tcaatgcctt ccgcctttag acttccctag ttcccgtctc aagtcaagca tttttttgaa
    12961 acagatatag tctaaccagt cactagcaaa agcgataagt agtaataata gccaaatacc
    13021 ccataacttg atatctgtca aatttctcaa gacgatattg aaaaacagaa ctgaaaaaac
    13081 tgtccaagca aggctaaaaa gagaatagag ggtgatgtaa atccagtaaa aatagtaaaa
    13141 aattgggaaa aatttacttt ctctattatc cttgtcaatc cagctgaaaa agtaaaaaca
    13201 gggtaataag attatcattt taaacaagtg ttccatcaca agcctccttt tttaaattgt
    13261 tttctttagt ttcgttctat taaatgttat taccaatata aaccttggaa tagcgtgtaa
    13321 taaatgattt tagaggtacc ataagccacc tcctacaaat agaaaccgat ataaatcaat
    13381 gccttccacc cttagacttc cctagttcct gtctcaagcg aaacatttct ttgaaacagg
    13441 aataagttaa ccaattcata ccaatagcta gcagaataaa aagaaaccaa atgccccata
    13501 acttgatatc tgtcacattt ctcaagacgg tattgaaaaa cagaactgaa acaactgtcc
    13561 aagcaaggct aaaaagagaa tagaagggga tgtaaaacca gtaaaaataa taaaaaattg
    13621 gaaaaaactt actatttctg ttggccttct caatccagtt atcaaaataa aagtacggtg
    13681 ctaaaagtaa gaatttaaac aaatgttcca tcaccgacat caccctcctt cttttgatag
    13741 cgttttctat cattttatta tatcaaaaaa atccggaact gtcattccag attctacttt
    13801 tttatctgcg ttttcttgcg atgagatgaa tcggtgttcc ctcaaaaaca aaggccttgc
    13861 ggatttgatt ttccaagaaa cgcaggtaag aaaagtgcat gagttcttct tcattgacaa
    13921 agatgacaaa ggttggtggt ttggttgcca cttgggtcgc atagaaaatc ttgagacgtt
    13981 ttcctttgtc tgtcggtgtt gggttgatgg caatggcatc catgatgaca tcgttcaaga
    14041 cagctgatgg aatacgtgta ttttgacttt cgctgatttg cttaatcatc tcaggaagtt
    14101 tgtggagacg ttgcttggtt aaagctgata caaagataat cggtgcgtaa ggcaggtatt
    14161 ggaactgctc acggatatct tcttcccagt ttttcatagt gtggttatct ttttcaagcg
    14221 tatcccactt gttgaccacg ataatcatcc ctttaccagc ttcatgagca aatccagcga
    14281 tacgtttgtc gtattcacga atcccctctt ccgcattgat gaccattaag accacatctg
    14341 aacggtcaat agcacgcatg gcacgcatga cagagtactt ctcagtattt tcataaacct
    14401 taccagactt acgcatacca gccgtatcaa tcatggtaaa ctcttgacca tctgtatctg
    14461 taaagtgggt atcaatagcg tcacgagttg ttccagcaac aggactggca atgacacggt
    14521 cttctcccaa gatagcatta atcaagcttg attttccaac gttaggacga ccaatcaagc
    14581 taaacttaat gacatctgga ttttcttctt catattcatt tggaaggttt tccacaatcg
    14641 catctagtac atcccctgta ccgataccat ggacagatga gataggcagt ggttcaccca
    14701 aaccgagggc atagaaatca tagatatcat ttcgcatctc agggttgtcc accttgttga
    14761 ctgcgaggat aactggtttg tgggtcttat aaagcttacg agctacgtat tcgtctgcat
    14821 cagtaattcc ttccttacca gacacgacaa aaacgataac atctgcttct tccatggcaa
    14881 tttctgcctg gtgcttgatt tgttccatga aaggagcatc gacatcatca attcctcctg
    14941 tatcaatcat gctaaaagaa cgattgagcc actcacccgt tgcataaata cggtcacgtg
    15001 tcactccttc gacatcttct acaatggaga ttcgctcacc agcgatccga ttaaataggg
    15061 ttgatttccc aacattggga cgtcctacaa tggcaatagt tggtagggcc ataatttctc
    15121 actttctaca ataatttctt ctgttcaaga ttttttctag ttgagcttgg ttcagcttga
    15181 ccaaactgtt ctgctagacg ctgactccag cttgtggtcg cacgcgcccc agcatagtca
    15241 gcctgaacac ggtcataagc ttggattgcc tcagttgact gttcttggta ttcttcctca
    15301 aagacaacat tctctagtgg cagtctcggt ttcatatcat gatgttgatt tggcacaccc
    15361 agtgccatcc caaagacaga ataggtgtag tcaggtaggt taaagagctc tgccacttct
    15421 tcagacttgt atcgaaccaa accgataatc acaccaccat agcccaagct ttcagctgcc
    15481 aacaaggcgt tttgtccagc aagagctgca tcgaccgaac taatcaagag accttccaca
    15541 ccttggggtt ggaaggtgtc ggtatgaagt cgggctccct tttctgctcg gttcaaatct
    15601 ccgacaaaga gaaggaaaac agcagactgg cgaatggctt cttgaggtac caattcatac
    15661 aaggcatctt tcttctcttg acttcgtacc acaatcacag agtaggattg gaaattcttc
    15721 caagatgatg ccatctgggc tgctgtcaaa atctcattta agtctacttg gggaatttct
    15781 tgctctttaa acctgcgcac tgaagtatga gccttcatca atttaatggt ttctgtcatc
    15841 gacggtttac tccttctaaa cgagtctcct cagccaaata acggatgcgt tccatgacac
    15901 gtctggcttc ccaggtttcg tcatttccat ttttcccttt agcgaaatgc ttctccaaat
    15961 cttcaaagtt gaagttagag gtgaaaaagg tcggtaaatt ttcctgcatc cgatattgga
    16021 gaatgacctg caggatttca tcacgcaccc aagcggtgga ttgctcggca ccaatatcat
    16081 ctaaaatcag gacttcagaa aacttaatct catccaccaa ggtcttaaca ttgccatcac
    16141 tgatagcatt tttgacatca atgacaaagc taggatagtg gaggagagtt gatgaaacac
    16201 cacgtttttc tgataaatca tgagctaagg ccgccaccat gaaactttta cccacaccaa
    16261 agtctccata taagtaaaga ccttttcgaa tagctggata ttgctccacg aaggctagta
    16321 gcttttcaaa aactggtaag cgccccaaat catccaagtc aacttgagcc aaactagctt
    16381 tcttgagact ggctggtaga ttgattaact tgagacggtt cttaatagcc gcttcttttt
    16441 cagccgcgat tagctcagga gtttcttcat atgaaacatc tgcatagcca tgattcttaa
    16501 ccaaaatcgg cttgtagcct ttggcaatat aatccgtatc cccacggaga aacttgtcac
    16561 gctcggtgat gtactgatta aacttggaga tactgcgatt taattcctct ggagtcaagg
    16621 attcttgctg gataaaagcc gcaacatcag ggtccttcat gattttctgg accaaatctt
    16681 gataataaaa acggctgggt tgacgtttga gtacgtctcc gacactttcc atctaatctc
    16741 ctcctttttc taatcgagct aatagttctt gcttcttacg ttctagttcc agacgagttt
    16801 cctcgctggt ttcattctta tattcaggat tactccattt aggaacattg gtttttactg
    16861 gggcagtctg attctgtttt tgtgtttttg ctttctgtcc tcgctcacgg atgcgcaaga
    16921 ctgccttttc tgccgaatga atcttttgat aggcatagtc attggctacc ttcatggcat
    16981 atttctcatt gatatttgcc gaatcgacct tattaaaggt caataaaaga atgatattga
    17041 tgacttcgtc cagcaagccc aagccagcca tctgttgcaa gagttctctt tctgtttggg
    17101 taatggttcc cttgcgtgtt tgcttgattt ctgccaagaa ctgcagggca gttttacttt
    17161 tggcttcttt gataatggtt gtttccttaa gactaaagtc agaggaaact ggtttttgag
    17221 caattttttc acgcatgcgt ttggttgaaa taacctgaga aacagctgtt gacttggcca
    17281 attgataggt ttcaaaccaa gtccatttct tctcctcggc aatagcaaag aggtttagga
    17341 catcggactg ctcatccgca aaacgaagtc catctcgagc catcagctgg cgaaaatgtt
    17401 ccaagtcaaa atcattggcc actttcttct tgagaccaag gtcttcttga ctgcctagtt
    17461 ctgccaattc tggaaagact tgattgagtg agacaggtat ttcttcacca tcagcacttt
    17521 caactttcaa atcctccaca gcagagtcgc caatcttttt ctctaagagt ctgcgataaa
    17581 caggatgctc caagaagtct tgactagata gaggagcatg gagggctagc tgataaacat
    17641 cccccttttg atagagggtc aagagattaa aagcagataa gattttcaat gattttatca
    17701 gtctatccat cccaaagttg agatgattga ggatacttga aaaaagatat tcctttctac
    17761 cattatccca aaaactgatt gtataaagat aaaggctcag tgcctcctga ccgataatcg
    17821 ggaggtagca ctgtaccaga gatgaggtat cttgcgacac ccgattattc tttagataag
    17881 aaaaacggtc aattggcttc atttatcttt cctttttctt tttagaggac tgggtgattt
    17941 gttggagcaa gctctctaac tcactgacat ccttaaaact acgatagaca ctagcaaaac
    18001 gtacataggt aatctcgtcc aattcagcca actcctccat gacgagtgaa ccaatgtcct
    18061 cactttgaat ttcattttca tttcgaccac ggagtttctg ttcgatacga ttgactacca
    18121 tgttgatttc atcacttgac acaggacgtt tctgggctga gcggataatc ccattaaaga
    18181 ttttatctct ggagaattgt tcccgtgtgc catctttttt aacaaccact aaggttcttt
    18241 cttctactcg ttcgtaggtt gtaaaacggt gttggcactc gtcgcactct cgtcttctac
    18301 gaatggtgtt cccttcttct gcttggcgac tatcgataac acttgacttg gtagccccac
    18361 attttggaca acgcatcctt tccctcctta tcgttttctt ttcattatac cattttttaa
    18421 gcgattccca aaacaattct tctttttgct tgacaagttt tttgttttgt tgtattattt
    18481 aattaagaca aaaggataag agaaaggaga ctaagatgtc ctggacattt gacaacaaaa
    18541 aacccatcta tttacagatt atggagaaaa tcaagcttca gattgtttcc catacactgg
    18601 aacccaatca acaacttcca accgtgaggg agctagctag cgaggctggt gtcaatccca
    18661 ataccatcca aagagcctta tcagaccttg aacgagaagg atttgtctac agcaagcgaa
    18721 caactggacg atttgtgact aaggataagg agctaatcgc tcagtcacgc aaacaattat
    18781 cagaagaaga attggaacac ttcgtttcct ccatgaccca ttttggctat gaaaaagaag
    18841 aactaccagg cgtagtcagt gattatatta aaggagttta agcctatgtc attactagca
    18901 tttgaaaatg tatccaaatc atatggagca acaccagccc ttgaaaatgt ttctcttgac
    18961 attccagctg gaaaaattgt cggccttctt gggccaaacg gctcaggaaa aacaaccctg
    19021 attaaactaa ttaatggcct cttacaacca gatcaaggac gtgtcctcat caacgacatg
    19081 gacccaagcc cagcaaccaa ggccgttgta gcttatttgc ctgatacgac ctatctcaat
    19141 gagcaaatga aggtcaaaga agccctaacc tacttcaaga ccttctataa agatttcaat
    19201 cttgaacgcg cccatcatct acttgcagac ctgggcattg atgaaaatag tcgtctcaag
    19261 aaactatcaa aaggaaacaa agaaaaggtt caactgattt tggttatgag ccgtgatgct
    19321 cgtctctatg ttttggacga acccattggt ggggtggatc cagcagcccg tgcttatatc
    19381 ctcaatacca ttatcaacaa ctactcacca acttctaccg ttttgatttc tacccacttg
    19441 atttctgata tcgagccaat cttggatgaa attgtcttcc taaaagacgg aaaagtcgtc
    19501 cgtcaaggaa atgtagatga tattcgctac gagtcaggtg aatccattga ccaactcttc
    19561 cgtcaggaat ttaaggccta agcaaaggag attatttatg ttttggaatt tagttcgcta
    19621 cgaatttaaa aatgttaaca agtggtattt agccctctac gcagccgtgc tagtcctttc
    19681 tgccctcatc ggaatacaga cacaaggctt taaaaatcta ccttaccaag aaagtcaggc
    19741 tactatgcta ctttttctag ctacagtctt tggtggcttg atgcttacac ttgcgatttc
    19801 aaccattttc ttgattatta aacgcttcaa aggtagtgtc tacgaccgac aaggttatct
    19861 gactttgacc ttgccagttt ctgaacacca tatcatcaca gccaaactaa tcggtgcctt
    19921 tatctggtca ttgattagca ctgctgtatt ggctctaagt gctgttatta ttctggcttt
    19981 aacagctcca gaatggattc ctctttctta tgtgattaca tttgtagaaa cacatctccc
    20041 tcagatcttt cttacaggta tatccttcct actaaatacc atttcaggaa tcctctgcat
    20101 ctacctggct atttccattg gacagctttt caatgaatac cgtacagcac tcgctgttgc
    20161 agcctacatt ggtatccaaa tcgtcattgg atttattgaa cttttcttca atcttagttc
    20221 taatttctat gtcaattcac tggtaggact caatgaccat ttctatatgg gagcaggtat
    20281 agccattgtt aaagaactca tattcatagc tatcttttat ctcggaacct actacatctt
    20341 gagaaataag gttaatttgc tttaaataat ttttacctag atatgtaaca tactcataga
    20401 acaaaagaga ccaggcaaaa agtctttaaa attagaaaac gcatagtatc aagtgttaaa
    20461 aatccttgat attatgcgtt ttttgtgagc tgacttattt cctttcgcta tatcgcaaaa
    20521 tgaaataaga acggaacgat gggattttgg aattcaaatc aatttataag aatgttttag
    20581 aagtaatatt atcctattcc agattcagtt cactatacaa ttgagttttc aagcaacctg
    20641 tttacataat gtgtacataa ttaggttcgt gattccaccc ttttcacctt taaaaacctc
    20701 gctttcgcaa ggctcttcta tttataagat aaggcacgtt taaaggtttt ccaaatccct
    20761 aaatcatccg tttgaagaac gagactagca tacatgcgtc cgataaatcc tgttgctacc
    20821 accgcaaaaa tcactgtaat agcaagtgaa atccatgctt ccgctccctc cgcatagcca
    20881 ttaatcgttc gaaacggcat aaagaaggtc gaaataaagg gaatataaga accaatcttc
    20941 aagaggagat tgtcaccagc tgcacctaga gctgtcactc caaaaaaacc acccataatc
    21001 aaaatcatca aaggcgacaa ggctttccct gagtcctcag gacgagaaac catagatcct
    21061 aggaaggctg ccaagactac gtacatgaaa agactgatca aaataaagag caaggtattc
    21121 agtgagatag catctcccaa gtgatccaaa ataccagact gagccaagaa tggcaaatct
    21181 ttaaagagca aaacggcagc cagaccacct acaacataga tcccaatatg cgttaaaatc
    21241 actagaaaca gagccatcat ccgcgcatag aaatagtgac ttgcccttat gctagaaaag
    21301 acgacttcca taattttggt gcctttttca ctggcaactt cctgagctgt tacacccgca
    21361 taggtaatca gaatcatata aagaaagaat cctaaggcac ctgctgcaat tgtttgaata
    21421 aactttttat tttccttggc ttcatcaatc ttttctgtga attgaattgt ctgcgctaag
    21481 cgtttttcct gctcttgaga caaggaagca gttgaacgat taagctgatt ttgcagttca
    21541 ttgagtgtac ctgtaacctc aaatttaatt ccattttcaa gcgatgtttc gccatgataa
    21601 actgccttta gaacactatc ttcttgatca atggtcaaat aaccttttaa tttttcttct
    21661 ttaattgctt ctttggcact tgcttcatct ttatagtcga agttaacacc atttacattc
    21721 ttcagtcctt ctgctacaga tggcactgtt gtcactactg ccactttatt atttttagtc
    21781 atagaagaac cttggagatg cccaattcct acagagattc ctaaaaagag gaacggcgaa
    21841 atcaccataa agaagaaact ccatgactcg acatgtcgaa gataggtttc cttgattaca
    21901 acccacatat ttctcatact tccactcctg attctagttt aaagatttca tcgatagttg
    21961 gcgcttgttg gtcaaatgtt gcgatatatt gaccttgagt caagattggg aagagttccc
    22021 ttccagcgct ctcatcctcc aaaatcaatt tccaactgcc ttgtttggtc aagctcacct
    22081 gtttgacatg aggaagattt tccaattctt ccttgcttcg ttcacttgaa acaaagagac
    22141 gcgttttccc gtattgattg cggacatcct gaactggtcc gtgcaagacc acacggccat
    22201 ctcggatcat cagaatatcg tcacaaagtt cctcaacatt ggtcatgaca tggtcagaaa
    22261 agataatggt tgctccgcgc tctttttcct gaaaaatgac ttgtttgagc aattctgtat
    22321 taactgggtc caatccacta aaaggctcat ccaagataat caggtctggt tcatgaatca
    22381 gagtaataat gagctgaatc ttctgctgat ttccttttga cagactcttg attttatctg
    22441 tcagctttcc tttcacttcc aacctcttca tccattgagg gagtttttct ttgacttctt
    22501 tggcatccat gccttttaga gtcgccaagt agcgaacttg ttcaagaact gtcaatttag
    22561 gcatgagact gcgttcttca ggcagataac caatccgagc ataggtctcc tgacgaatat
    22621 cctgaccatc cagaccgatt tctccctgat attctaggaa tttcaaaata ctatggaaaa
    22681 tcgttgtttt tccagcacca ttttttccga ctagtcccaa aatacgacct ggtcgcgctt
    22741 gaaagtcaat accaaacaaa acttgcttgg agccaaaact tttctctaga cttcttactt
    22801 ctagcatctt tcacctccga aatttcttgc actcattata ctcctttttg atagccttta
    22861 caatgttttt gtccattttt agaagactat tgctgtgtaa aatatggcct ggagcacttt
    22921 tatactcaat gaaaagcaaa gagcaaacta ggaagctagc cgtaggctgc tcaaagtaca
    22981 gctttgaggt tgcagataaa actgacgaag tcagctcaaa acactgtttt gaggttgtgg
    23041 atagaactga cgaagtcagt aactatatct acggcaaggc gaagctgacg tggtttgaag
    23101 agattttcga agagtattag tgataaatcc attatacagt agcaaactta atttatacct
    23161 tccgctcctc aactgtctat ttttaatcct gaattgttat ttgagtaact cctttttcct
    23221 cataaagttt tcttcctcta aaatttctgg aaaaaagcga atagtttcag acaacatttt
    23281 tataagaaac aagttcatct gtcatttcaa gaaggagtaa tcctttatct actaatggac
    23341 ggaacagaat tcaaccgctt gtccgatatg ttttctaagg attatataat aaaatgaaat
    23401 aaaaacagga caaatagatc gggacagtca aa
//
LOCUS       DAVKAO010000027        22932 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKAO010000027 DAVKAO010000000
VERSION     DAVKAO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.027"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            67..641
                     /locus_tag="VWX16_001093"
                     /pseudo
     CDS             67..641
                     /locus_tag="VWX16_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            638..856
                     /locus_tag="VWX16_001094"
     CDS             638..856
                     /locus_tag="VWX16_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158463.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(913..1197)
                     /locus_tag="VWX16_001095"
     CDS             complement(913..1197)
                     /locus_tag="VWX16_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9158464.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(1251..2186)
                     /locus_tag="VWX16_001096"
     CDS             complement(1251..2186)
                     /locus_tag="VWX16_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9158465.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            2290..2733
                     /locus_tag="VWX16_001097"
     CDS             2290..2733
                     /locus_tag="VWX16_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9158466.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2849..3115
                     /locus_tag="VWX16_001098"
     CDS             2849..3115
                     /locus_tag="VWX16_001098"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9158467.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            3112..3486
                     /gene="crcB"
                     /locus_tag="VWX16_001099"
     CDS             3112..3486
                     /gene="crcB"
                     /locus_tag="VWX16_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9158468.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDAVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            3480..3809
                     /gene="crcB"
                     /locus_tag="VWX16_001100"
     CDS             3480..3809
                     /gene="crcB"
                     /locus_tag="VWX16_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9158469.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            3928..4275
                     /gene="rplS"
                     /locus_tag="VWX16_001101"
     CDS             3928..4275
                     /gene="rplS"
                     /locus_tag="VWX16_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9158470.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            4317..4388
                     /locus_tag="VWX16_001102"
     tRNA            4317..4388
                     /locus_tag="VWX16_001102"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4350..4352,aa:Arg,seq:cct)
     gene            complement(4443..5249)
                     /gene="yidA"
                     /locus_tag="VWX16_001103"
     CDS             complement(4443..5249)
                     /gene="yidA"
                     /locus_tag="VWX16_001103"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9158471.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETDTGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYTTKTNDESGVAHAIRTWVL"
     gene            complement(5262..6566)
                     /locus_tag="VWX16_001104"
     CDS             complement(5262..6566)
                     /locus_tag="VWX16_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9158472.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQCNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            6638..7015
                     /locus_tag="VWX16_001105"
     CDS             6638..7015
                     /locus_tag="VWX16_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9158473.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDRQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            7105..7437
                     /locus_tag="VWX16_001106"
     CDS             7105..7437
                     /locus_tag="VWX16_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9158474.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILDRYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            7449..9020
                     /gene="ffh"
                     /locus_tag="VWX16_001107"
     CDS             7449..9020
                     /gene="ffh"
                     /locus_tag="VWX16_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9158475.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKVLAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTSEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(9215..10498)
                     /locus_tag="VWX16_001108"
     CDS             complement(9215..10498)
                     /locus_tag="VWX16_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9158476.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(10752..11465)
                     /gene="rsmG"
                     /locus_tag="VWX16_001109"
     CDS             complement(10752..11465)
                     /gene="rsmG"
                     /locus_tag="VWX16_001109"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9158477.1"
                     /translation="MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            11559..12119
                     /locus_tag="VWX16_001110"
     CDS             11559..12119
                     /locus_tag="VWX16_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9158478.1"
                     /translation="MTWIILGVIALIVVFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            12121..13020
                     /gene="htpX"
                     /locus_tag="VWX16_001111"
     CDS             12121..13020
                     /gene="htpX"
                     /locus_tag="VWX16_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9158479.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRIFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINAPKKG
                     GGVQKLFYTHPPISERIERLKQM"
     gene            complement(13169..14728)
                     /locus_tag="VWX16_001112"
     CDS             complement(13169..14728)
                     /locus_tag="VWX16_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9158480.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIICRMTE
                     HGLKLVNLEDL"
     gene            complement(14967..15284)
                     /locus_tag="VWX16_001113"
     CDS             complement(14967..15284)
                     /locus_tag="VWX16_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9158481.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYRPV
                     FEF"
     gene            15454..15996
                     /locus_tag="VWX16_001114"
     CDS             15454..15996
                     /locus_tag="VWX16_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9158482.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            15996..16625
                     /gene="nth"
                     /locus_tag="VWX16_001115"
     CDS             15996..16625
                     /gene="nth"
                     /locus_tag="VWX16_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9158483.1"
                     /translation="MILSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVVFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            16836..17357
                     /gene="pyrR"
                     /locus_tag="VWX16_001116"
     CDS             16836..17357
                     /gene="pyrR"
                     /locus_tag="VWX16_001116"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9158484.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            17376..18299
                     /locus_tag="VWX16_001117"
     CDS             17376..18299
                     /locus_tag="VWX16_001117"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9158485.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQIPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFSGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            18349..19428
                     /gene="carA"
                     /locus_tag="VWX16_001118"
     CDS             18349..19428
                     /gene="carA"
                     /locus_tag="VWX16_001118"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9158486.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            19741..22917
                     /gene="carB"
                     /locus_tag="VWX16_001119"
     CDS             19741..22917
                     /gene="carB"
                     /locus_tag="VWX16_001119"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9158487.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELSVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAVVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         6650 a         4378 c         5143 g         6761 t
ORIGIN      
        1 gacagtcaaa tcgatttcta acaatgtttt agaagtagag gtgtactatt ctagtttcaa
       61 tctactatat ttcgtctgat gggcaaatct tataaagaga ttatagatct tttagactgt
      121 aatcaaacaa cgatttggcg aaatgtaaaa aaatatgagg agttcggact cgactctctc
      181 cttcaagaaa cacgtggtgg tcgtaaccat gcatatatga cagttgagga agagaaagcc
      241 tttcttgccc gtcatttgaa ggctacagag gcaggagaat ttgttacaat tgatgcctta
      301 tttcaggctt ataaaaagga gtgtggatga acgccttttt agaagagctt tcacaagctt
      361 atccttttat tcgttatgga caatgctata tggcataaat caagtacctt aaatattccg
      421 actaatattg attttacctt tattcctcca tacacaccag agatgaaccc attgaacaag
      481 tgtggaaaga gattcgtaaa cgtggattta agaataaagc ctttcgaact ttggaagata
      541 tcatgaatca acttcaagat gtcatacaag gactggagaa ggaggtgata aagtccatcg
      601 ttaatcggag atggactaga atgctttgtg aaagcagatg agtataagtc ctcgttttga
      661 aaccttagag caggccattg cctccaaaga tttggaaaaa gtccgagaag cttttaagaa
      721 aatgaattcc acttggacca tcaatgaaag tgtggttcgt gacaatagta cagctcatta
      781 tggacgtgtt gaaacagcta tttccttcct gcctagtagc atggaaaccg aacctacaga
      841 tgagtctgga acatgaaaac agcagttgat ttcaagaaac aaaaaagaac agccgaaact
      901 gttctcttat cattatttga gaccgtattt tttgttgaaa cgatccacgc gtccatctgc
      961 ttgagtgaac ttttgacgtc cagtgtagaa tgggtgtgag tctgatgaaa tttccacacg
     1021 gatcaatggg taagtttcgc cttcgaactc aactgtttcg ttagagcgtt ttgttgaacc
     1081 gctaaggaat tggtaaccag tagttgtgtc catgaagaca actgggcgat attctggatg
     1141 gatatctttt ttcattttgc aatatttcct ttctgccatg gtctctttgc gagccataga
     1201 ttgttaccat actagtctat cagattctga aaagtttggc aagtatttta tcagttttta
     1261 agcaagtttt ttaacttttg gtagatgatt tcgttttctt ctaggctata ggaattagca
     1321 ccacttgcta gagggtggcc tccaccatca tgctccttgg cgatttcatt gataggatgg
     1381 attttactgc gtaagcgaac tcggtagtgg ccatcagcct gttcgacaaa aattccccag
     1441 agactcactc tgtcaatgcg tccaggtgca ccgacaatgg ctgcagtttc agcatcggtt
     1501 acattgtatt gtttcaagac ttcttgactc aggataacgc gagcagcacc attttcatcc
     1561 acttccagat ggtcgtagat gtagccttgc agtttagcaa ttttgtagct catagtgtcc
     1621 attttgcgag tgagagccgc aaagtcaaag ttatgttctc tcaaataagc agccaggcga
     1681 agagtccgtg cagtggtaga agggtagagg aagcgacctg tatcaccgac aattcctgca
     1741 aagagcaact cagcatcgcg atctgccaag gctagttggg ttgtttgggc aaatagggta
     1801 atcatctcgc tagcgctact tgaactagta tcgacccaag acaggtcacc gtatacatca
     1861 tcatttggat ggtggtcaat cttaatgaga aaatcacctt gactatagcg cttatcatcg
     1921 atacgagcag tattagctgt atcacagacg atgacaagtg cgccttggta ggctctatct
     1981 tcaacaagat ccatctcagc catccaagta agagttggtt catcaaaacc gacggctttg
     2041 atggtttttt ctgggaaatg atgttccagc aaggctttca atcccacctg acttcccaag
     2101 gcatcagggt ctggtttcat atgacgatga atgataatgg tgtcgtattc tttaattttt
     2161 tctaaaattt gttggcaaat ctccataaat aacctcaatt ctttctcatt tcattgtagc
     2221 acaagaattt ttatttttaa aatgaaaaag atgtgatata atggagaaag ataaattgag
     2281 gaggacgata tggcattagc aaaaattgta tttgccagta tgaccggtaa taccgaagaa
     2341 attgcagata ttgtagcaga caaattacgt gacttgggct tggatgttga tgttgatgaa
     2401 tgtacaactg ttgacgcttc agacttcttg gaagcagaca tcgctatcgt tgcgacctat
     2461 acttatggtg atggtgaatt gccagatgag atgatggact tctacgaaga cctagcagat
     2521 ctcaacttga atggcaaaat ctacggagtg gtcggctcag gagatacctt ctacgatgaa
     2581 ttctgtaagg ctgttgatga ctttgaccgt gtctttgtgt caacaggagc agaaaaaggt
     2641 tcagagtgtg ttaaagttga tctttctgcc gaggaagaag atattgaacg cttggaacaa
     2701 ttcgcagaag aattggctgc taaagtagga taagcataaa agtgcgctga tgtaatcaat
     2761 cagtgcattt ttcttatcga gagttgggat tttactagcc taattgatgg ctttttgata
     2821 caataattca aataaaggag gagactgtat ggatttagat attattcgtc aagaaatcga
     2881 tcaaatcgac gaccaaattg tcaaactgtt agaagaacga atgcatttag ttgagggggt
     2941 ggttgcttat aagaaagctt caggtaaacc gattttagat actaagagag aagaagttat
     3001 ttttgaaaag gttaggagtc gtgtagagga caagcgctat caggagacta ttgtagcgac
     3061 tttttcggac atactcaaac gttcgcgtga ttatcaggat caaaatatca aatgaaaaaa
     3121 gaacaatttt atccgctagg aatttttcta gctgctatgt tgggcggact tgtccgatat
     3181 ctagtttcca cctggttacc agccagtcca gactttcctt ggggcactct ctttgtcaac
     3241 tatctgggaa ttttctgctt gatttttctt gtcaagggct atctggtcta taaggggact
     3301 agtaagggct tgattttagc actggggacg ggtttttgcg gaggtttaac aaccttttct
     3361 agtctcatgc ttgatgctgt gaaattactg gatacagggc gttatcttag tttgatactg
     3421 tatttgcttt tgagtatcgg tggaggcctg cttttagctt actatttggg gaggaagaaa
     3481 tggtaatcgt ctatcttgca atcgcctgtg gcttgggagc actcgtacgg tatttctttt
     3541 cccgctacaa tcaagcttct aaattgccac ttggaacgct catagctaat cttttaggtt
     3601 gttttttaat tggagtattc tacaatcatg tagagtctaa ggaagtatat gctattctag
     3661 caacaggatt ttgtggcggt ttaacaactt tttcgacctt gaatgacgaa cttcaaagac
     3721 tgctaagtga taagaaggtc ttttattctt acctgacttt gacttacata ggtggtttgg
     3781 ttgcgatttt tttaggaatt ttgctataaa tttctttact tttttgtgaa aatttggtag
     3841 actgtatctt gtgtgtgatg gacgcacaaa aatacagttt atccgctgag gaagtttcct
     3901 caagattgac aaagatagga gaataaaatg aatccattaa tccaaagctt gactgaaggt
     3961 caacttcgta cagatatccc atcattccgt cctggtgaca ctgttcgtgt acacgcgaaa
     4021 gttgtcgaag gtaaccgtga acgtatccag atttttgaag gtgttgttat cgcacgtaaa
     4081 ggtgctggaa tctcagaaaa ctacacagtt cgtaaaatct ctaacggtgt aggtgttgag
     4141 cgtatcttcc caatccacac tccacgtgtt gaaaaaatcg aagttgttcg ttacggtaaa
     4201 gtacgtcgtg cgaaattgta ctacttgcgt gctcttcaag gtaaagcagc tcgtatcaaa
     4261 gaaatccgtc gttaattcga ctaaaaaact ctgcttttca gcagagtttt tatctagctc
     4321 ccttagttca atggatataa caactccctc ctaaggagta gttgcaggtt cgattcctgc
     4381 agggggcagt aattagacat caaaaaccgc tcagctgagc ggtttttatc tattctttcc
     4441 ttttacagta cccatgtacg aatagcatgg gcaacgccgg attcgtcatt tgttttggtg
     4501 gtgtatttgg cgattttttt gatttctgga tttccgtttt ccatgactac agggttgcca
     4561 acgacttcca gcatggcacg gtcattttct tcgtctccga tagccatggt ttcatctttg
     4621 gttaatccta gtttttcagc taagtgagta atagctgaac ccttgtctac attcttttta
     4681 aggagttcga ggtagaaagg agcagatttg ttgatagagt agtgctcgta aaattcggct
     4741 ggtatttttt caatcgcagc atcgaggatt tctggttcat cgatgaacat acacttgaca
     4801 atttccttgc cagccatttc ttcaggagtg cggtagaaga taggcatgct gacgagggtt
     4861 gattcgtgca cagtgtatgt tccgatattg cgattggcgg tatagatacc gtccttagta
     4921 atagcatgca tgtgaacacc aagcttgcga ctgagaaatt ccatatccag ataatcctca
     4981 taggtcaagg attcgctgat aatctcatgg ccagtatcag tctcttggac aagggcacca
     5041 ttgaaggtca ccacatagtc accttggtct ctcaactgca agtcgtccag aagtttggca
     5101 acacctgcga tggggcggcc agttgcaatc acgactttga caccagcttc tttggcatct
     5161 tggatggcag aaaagacttc aggagtgatt tccttttggc tgttgactag ggttccgtca
     5221 atatcgacgg caattagttt aatactcata aaattcctct tctagttttt atttggggta
     5281 aaatgatcgt tctcaatcaa gtgtaaaaat tgctgggtaa tgcttgcaaa gatgctgttt
     5341 tggtccaaca tttcttttgg aaaataaaag cgattatctc cgtggcgact gccagcaagg
     5401 gattggacga taggagacag gctagagagt tcggccagtt ctccattttt ttgtaaaatc
     5461 tcaatctgtg tccgtgggtt ttcagattcg ggacgataga tatcataagg gaggtcaaag
     5521 ttcttatgaa tggcagtgta gtagtcggga tcaaagccga tatcctcaac caattttctc
     5581 atgctagtaa gttgatcttg gtcctcttgt gaaaaggtaa tggatttaaa gaccttgcgg
     5641 ttgacaaagc gatgcgataa atctgcaaga atcttgtcag gactggtcat ccaaagctgg
     5701 aagtaggtat tcatcacgcc atcatccaga gccagatagt cagtcaaggt cacatttttt
     5761 tcgaagaaag gcaggaggtg tggagaagtt cgggcaaaga aatccttgtc ctcaggatag
     5821 agttccttgg cgcgtttgag aagattctgt aggagaactt ccatggcgcg tgttgcgggg
     5881 tggaaataaa cctgcatgta catctggtag cgactgagga cgtagtcttc gatggcgtgc
     5941 atgccattgc actgaaaggc gataccattt tcgataggac gaatgactcg gaggattcga
     6001 gtcaggtcaa attccccata ggatgctcct gtaaaatagg agtcacgcaa gagatagtcc
     6061 atgcggtccg catcaatctg gctagaaatg agctgcacga cctgcttatt aggataggta
     6121 tggtcaatga cactggccac cttttctggg aaatcaggtg ccacttgtag caggacttgg
     6181 tgaatctctg tctcaggatt ttgaataatc tcctgagtaa tggcttcatg gtctgtatca
     6241 aagagatttt caaaagtatg ggagtaggcg ccatgcccaa ggtcgtgtag gagagcagcg
     6301 gtcatggtca agagagactc ggcaggattc cattcctcag gatatttttc ttcgaaaatc
     6361 tctgtgatgc gtcgtgcaat ttcatagact cctagacagt gagagaagcg actgtgttct
     6421 ccaccgtgga aggtataact ggaagttccc agttgtttga tccggcgcaa acgctgaaat
     6481 tcttttgtat taatcaagtc atagatgatt tgattattga catggatgta gttgtgaact
     6541 gggtcacgga atactttttc gttcatatga ccattatagc aaaatcctgc tctttttggt
     6601 aaaatagtag gacaagagac aagaaagagg acttgatgtg aagattcgga tgcgaaatac
     6661 gattcagttt gatgagcagt tggaagtgat tgaccagctt tatgatgtgg aagtgcatga
     6721 aaagggagat tatagctatt tactctttta taatgaggaa aaggaaaaag tagttattaa
     6781 atttcatggt caagaactgg tgatgagccg attttctaat cccaagacca ttatgcgctt
     6841 tttaaaggat agtgatagtt tggcctatat tcctacccct atgggcatgc aggaatttat
     6901 cattcaaacg agccgttatc aagttgatag gcaaaagatt gagctagctt atcaactaca
     6961 aaatcaagag gggcatccct ttgccagcta tcaattggaa attacttggg gctaggatct
     7021 cgtttttctc aaatctactt tctatcttca aggattggct ccctagtgtg gtagattagg
     7081 ctattttttg gtataataaa agttatggaa atcgaaaaaa ccaatcgtat gaatgcgctc
     7141 tttgaatttt atgcggcgct tttgacagat aagcaaatga attatataga gctttactac
     7201 gctgatgatt acagtcttgc tgagatagct gaggagtttg gtgttagtcg tcaggctgtc
     7261 tatgacaata tcaagcgaac agaaaagatt ctggaagatt atgagatgaa attgcacatg
     7321 tactcggact acattgtccg tagtcagatt tttgaccaaa tcttggatcg ctatcccaag
     7381 gatgattttc tgcaggagca gatagaaatt ttaacaagca ttgataatag agaataagag
     7441 gaagaaaaat ggcatttgaa agtttaacag aacgtttgca gaacgtcttt aaaaatctac
     7501 gtaaaaaagg aaaaatctct gaatctgatg tccaagaggc aaccaaagaa attcgcttgg
     7561 ccttgctcga ggccgacgtt gccttgcctg ttgtaaagga ctttatcaag aaagttcgtg
     7621 agcgtgcagt cgggcatgag gtcattgata cacttaatcc tgcgcaacag attattaaaa
     7681 tcgttgatga ggaactgaca gccgttttag gttctgatac ggcagaaatt atcaagtcac
     7741 ctaagattcc aaccatcatc atgatggttg gtttacaagg ggctggtaaa acaacctttg
     7801 ctggtaaatt ggccaacaaa ctcaagaaag aagaaaatgc tcgtcctttg atgattgcgg
     7861 cggatattta tcgtccagct gccattgacc agcttaagac cttgggacaa cagattgatg
     7921 tgcctgtctt tgcacttgga acagaagtac cagctgttga gattgtacgt caaggtttgg
     7981 agcaagccca aactaatcat aacgactatg tcttgattga tactgcgggt cgtttgcaga
     8041 ttgatgagct cctcatgaat gagcttcgtg atgtgaaagt attggctcaa ccaaatgaaa
     8101 tcttgcttgt cgttgatgct atgattggtc aggaagcagc caatgttgcg cgtgagttta
     8161 atgctcagtt ggaagtgact ggggtcatcc ttaccaagat tgatggtgat actcgtggtg
     8221 gtgctgctct gtctgttcgt cacatcactg gaaaaccaat caagttcact ggtacaggtg
     8281 aaaaaattac agatatcgaa accttccacc cagaccgtat gtctagccgt atccttggca
     8341 tgggggatat gctcactttg attgagaaag cttctcagga atacgatgaa caaaaagccc
     8401 ttgaaatggc tgagaagatg cgcgaaaaca cctttgattt taatgatttc atcgatcaat
     8461 tagatcaggt gcaaaatatg gggccgatgg aagacttgct caagatgatt ccaggtatgg
     8521 ccaacaatcc agcacttcaa aacatgaagg tggatgaacg ccagattgct cgtaaacgtg
     8581 ccattgtgtc ttcgatgaca tctgaagaac gtgaaaaccc agatttgtta aatccaagcc
     8641 gtcgccgtcg tattgctgct ggttctggaa atacattcgt cgaagtcaat aaattcatca
     8701 aggactttaa ccaggctaaa cagctcatgc agggtgttat gtctggggat atgaataaaa
     8761 tgatgaagca aatggggatt aatccaaata accttcctaa aaatatgcca aatatgggag
     8821 gaatggatat gtctgccctt gaaggaatga tgggacaagg cggtatgcct gacttatcag
     8881 ctctcggagg agcaggaatg ccagatatga gccagatgtt tggtggcggt ttgaaaggta
     8941 aaattggtga atttgccatg aaacagtcca tgaaacgtat ggctaacaaa atgaagaaag
     9001 cgaagaagaa acgcaagtaa gacaagagat tgatgcaaat ctcatttgat gagaaaataa
     9061 agtaaaaatt tctatgataa aacgtacagt atcaaggttt tagaatatct gatattatac
     9121 gttttttgat tttaataatt ttgttaagac acttttatta tggtatacga aaaatccact
     9181 cagctgtctg agtggattag gtgtatttag actcttagtc tttattttcg tatggcaaga
     9241 tcaagttcaa gatgattcct gtcatggctg aaagggcagt acctgaaagt gtaactggac
     9301 caagtttaag gatagctcct ccaagtccaa gaaccaacat agcacttgcg atgatgaggt
     9361 ttcgcatttg agcgaaatca acacgttctt taatcaagac tttcaaacca ttgctggcga
     9421 taaccccata gagaaggatt gacataccac caagtacagc gtttggaata gttgaaatca
     9481 aggcagtgaa tttaccaagg aagctgaggg caatcgcgat gaaggcagcg ttacggataa
     9541 ctgagacaga agcgatacga gtcataccga taacccctgt attttctccg taagttgtat
     9601 tggctggtcc accaaggaag gcagaaacag aagttgcgat accgtcacca ataagagtac
     9661 ggtgaagacc tggttctttt aagaattgac ggccacagat ttgacccaaa acagtatggt
     9721 ctccgatatg ttcagaaatt gttacgatag cgattggcaa gatagcgatg gcttctggac
     9781 caaagtaaag attgtactct ttaaaggcac cacctgtgct aaatggcaag tagaaaccag
     9841 gaatttcgaa ccagttggct ttaagaactg gtgtaaagtc aaccaagcca agagttagtg
     9901 cgaaaaggta accaccgata atggcaaaga ggaatggaat gattcgtagg aagccttttc
     9961 cttttgtatt gataaaggca gcaattagga aagtaacaac ggctaccaga gcatttttcc
    10021 aatttccgtc tgctacaaga cctgcattgg taacagctga acctgcaagt ccaagaccga
    10081 taacgatgat cataggaccg ataatgattg gtggcaagag tttatcaatc cattttgttc
    10141 ctacaaatcg gatgctggta gcaacaagga catagaccaa accagtcaag ataacccctg
    10201 tttgggcagc agatacatcc ccccccattt ctttcatagc cagtgacata gctgtgataa
    10261 aggcaaatga agaacctaga taaactggaa ctttaaaacc agtagcaatc atgtagatga
    10321 gtgttccaac acctgaagca aagagggcaa cagatacagg cattcccaaa atcaatggta
    10381 ccaagatggt cgcaccaaac atggcgaaaa cgtgttggaa gctaaggaga attccttttc
    10441 catccgaagg acgttggtca acgtctagta acaaatcaac agttgattct tgtttcatat
    10501 aaacctcact tttgggcatc agaaaagagc ccattatttt acagcaatag gccctcagag
    10561 taagacttct ctaaaatcta gactttaaag agtttcaaat atttttgcgt cttcgtcacc
    10621 tcacgggatg acattaaaaa cctttgctta ggatagtata gcagaaaaaa atgattttgt
    10681 aaatcatttt ttcccgagcc tagaaataaa agagcgaggc tgattttgta aacatttgtt
    10741 tactaaaaaa tttaaagtgg gcgtttattt ggcataccag ccttacgtgg atatttattt
    10801 ggtgtttctt ttttcttttc taccactgtg atatagcgcg gatctctatt cggtagggcg
    10861 tagctgagat tgtcttcgac cttactaaaa aggagattga gggcattctt agcttctaat
    10921 aattcctcag gcgcattgct ggccttgagt gccaatagtt tgccaccaac cttaaggtag
    10981 ggaatagtca attcagatag gacctgcata cgggcaaccg cacgagctgt tacaaaatca
    11041 tattgagcac ggaagttctt gtcttgggca aaatcttcgg cacgtccgtg gtagaaatga
    11101 actccgttca aatccagttc ttgagccaag agttgtagga agttgatgcg cttattgagt
    11161 gaatcaataa tggtcacatc taactccgga tagagaattt tcattggtag actaggaaat
    11221 cctgccccag ccccgatatc aagaagtttg atagtttcat tgggaatcaa accttgaaga
    11281 atgggtgcaa tcgaatcgta aaaatgtttg agataaactt cttccttgtc cgtaatcgcc
    11341 gtcaaattaa tcttctcatt ccactcgacc aagagctcaa aataacgttc aaattgttct
    11401 ttttgctggt tcgaaagtgg aagattttgc tcggcaagca agttgtaaaa tgtttctggt
    11461 ttcatagtta tttccttctt tagtatcatc ttattttacc acaatcatta aaaatttgat
    11521 atactggtac taatctaaaa gtagaaagga cttatatcat gacttggatt attcttggag
    11581 ttatcgctct tattgttgtt tttgtgattg ttagctataa cggtttggtt aaaaatcgta
    11641 tgcaaaccaa ggaggcttgg agtcagattg atgttcagtt gaaacgtcga aatgacctct
    11701 tgccaaactt gattgagact gtaaaaggtt atgccaaata tgaaggttct acccttgaaa
    11761 aggtggcaga actacgtaac caagtggcgg cagcgacttc accagcagaa gctatgaaag
    11821 ccagtgatgc cctcactcgt caggtttcag gtatttttgc agttgcagaa agctatccag
    11881 atttgaaagc tagtgctaac tttgttaaat tgcaagagga gttgacaaat acagaaaata
    11941 aaatttctta ctctcgtcaa ctctataaca gtgttgtcag caactacaat gtaaaattag
    12001 aaactttccc gagcaatatt atcgctggaa tgtttggatt taaagcggca gatttccttc
    12061 aaacacctga agaggaaaag tcggttccta aagttgattt tagcggttta ggtgactaag
    12121 atgttgtttg atcaaattgc aagcaataaa cgaaaaacct ggattttgtt gctggtattt
    12181 ttcctactct tagctcttgt tggttatgcg gttggttatc tctttataag atctggactt
    12241 ggtggtttgg ttatcgcact gattatcggc tttatctacg ctttgtctat gatttttcaa
    12301 tcgacagaga ttgtcatgtc catgaatgga gcgcgtgagg tggatgagca aacggcacca
    12361 gacctctacc atgtagtgga agatatggct ctggtcgctc agattcctat gccccgtatt
    12421 ttcatcattg atgatccagc cttaaatgcc tttgcgacag gttctaatcc tcaaaatgcg
    12481 gctgttgctg cgacttcagg tctactagct atcatgaatc gtgaagaact agaagctgtt
    12541 atgggacatg aagtcagtca tattcgtaat tatgatatcc gtatttcgac tattgcagtt
    12601 gcccttgcta gtgctatcac catgctttct agtatggcag gtcgtatgat gtggtggggt
    12661 ggagcaggtc gcagacgaag tgatgatgac cgagatggaa atggtcttga aatcattatg
    12721 ctagtggttt ccctactagc tattgtactg gcacctctcg ctgcaacctt ggttcagctc
    12781 gctatttctc gtcagaggga atttctggca gatgcatcta gtgtcgagct gactcgcaat
    12841 ccccagggaa tgattaatgc cctagataag ttggacaata gcaaacctat gagtcgccac
    12901 gtcgatgatg ctagcagtgc cctttatatc aatgctccca agaaaggtgg gggggtccaa
    12961 aaactctttt atacccaccc acctatctca gaacggattg aacgtttaaa acagatgtaa
    13021 aatgaaggct ggaaaaaagt ctttaaaatc tgaaaaatgc ataatatcag gtgtgaaaac
    13081 ttgatattat gcgttttact atgggaagat ttacttcttt ttctcctaaa attgtgtttt
    13141 tgccccacct atctgctatg ttgcaaattt ataaatcttc taaattaact agcttcaaac
    13201 cgtgttctgt catgcgacag atgatcgagc agacttcttt taagaaacga cggtcatgcg
    13261 aaacagtgat gagaccgcct ggataggttg caaagagttt tctgatttgg ggttgagaag
    13321 tgggagaaaa gtttcgtgtg ggttcatcca gcaggagaaa gtttggtttg cgcaggacta
    13381 gatccaaaag caggagtttt ccctgttgtc cgccagataa ggagcgaatt tgatgctgca
    13441 tttctggata actgaaattg agactggcta ggtgagattg aattttctga agttcctctt
    13501 tttccccagt tttgctgata taagctattg gtgataaatc caattgtagt tttttgtggt
    13561 aatcttgtgg cataaaacca agggaaatct ctcttttatc attcagaagt tgctttaatt
    13621 tggctaacag agttgatttc ccaacaccat ttggcccgat aataccgatt ttttcttgcc
    13681 cacggacagt tagttgtagt ttttgaacca aaactcggtc gtcaatggac aaattttctt
    13741 tttccagttg gactaagact ttagaagctg gtaatggttg gatatctgaa aagaaaagtt
    13801 ggatttgttc ctcttcaagt ggtttttgag tcatggactg agctgccttt tcgtagcgtt
    13861 tttcttgtga gaggacagtt ttcatctttt tagccaatag gcgaccggca gtactatctt
    13921 tggtagctcg aagcgcagtt tctacatttt gcttaactct ccgatgtttt tccatggttt
    13981 tatcgtaggc tctttggttg ttagcagctt gctgactttg tttggcaaaa ttagcctttc
    14041 tctgctcact atagctatca tagtctaaat gctctactag cgtttccgct tctttacggt
    14101 gtttgaccag tcgcaagtga acaatagtgt ctgccgtttc agaaagaaag tcttcatcat
    14161 gggaaatgaa aataacggtt tgcctggtct tttgaatctg gccttttagc caatcaactg
    14221 tctcaaggtc taggtcattt gaaggttcat ctaaaaatag aatctcaaag ggtttggcta
    14281 actcatggat aagctgaatt ttcaaagctt ctccccctga tagattgcca atctcttggt
    14341 cacttgcgaa acgattgcta tcaaaatgca attcctccgc caaacgatag aggatactgt
    14401 agtctaaatc aatagaatct aaaaagaagt agtcgtgtaa agttttcttt ttcagctcct
    14461 cgggaagttt ttgaggaatg taggccagtg actgatagtc agattggatg tttcccttga
    14521 tagtgaaatc agacaaagct tcccccatta aaattttaag taaggttgat ttgccatttc
    14581 cttcttcacc aataatagca accttttccc cgtcttggat ggtcatgctt aggtcagata
    14641 caagatctcg taaatctttg ttttgtgtga tggttagatg attgattttt atcatatgat
    14701 ttccctttct agaatgttca tagtgcataa caaaaagctc tactttacct agtagagccc
    14761 aaagtctatg tcaaaaaaac tctattaaca tgccagaaaa agagcataaa agagcacaca
    14821 aaaagagaca aaaacaagat ctactaaata aaggttcttt atttgataga cttagttgtt
    14881 catgtctccc ttacctccga gtattagtat ctttatatta tacctttaag gaaattttgt
    14941 caacagaaaa ctggaaattc tatgctctaa aattcaaaaa caggtctata gagacttagt
    15001 acgtgtttga catgctcgta atggaattta tcatagtttc gccaagcagt gcgtgcttga
    15061 tcgtttagtt ttaggctacc cagtccaatc agaagactgg tgggttggta aaatttctca
    15121 agatcaatta agatactgtt gttcaactgt tccgtttttt tgagctcttg catagtagtg
    15181 tttagcagct cttgtagtcg aatatcatct gccgtaccct gtttacagtt ttggtagctt
    15241 ttatttaact tggataggag ttgaatgagt ttcttgtctt ccatgtgagc atcctccttg
    15301 atctgatgta ctctttccct atagtatagc actaaaaaac aaaactgtca tagtaaatat
    15361 gttaaagttt tgaattttaa ttacaaagtt tggtgtgctg atttgtagcc ttttcatggt
    15421 ataatcaaga gagtaaatct ttatggagga agtatgaaat taaatattca agaaattcgt
    15481 aagcagtctg aaggtttgaa ctttgaacaa acgttagatt tagttgatga cctgcgtgca
    15541 cgtaatcaag aaattttaga tgtaaaagat atccttgcag ttgggaaagt acaatatgaa
    15601 gaccgtatgt atttcttaga ttatcaacta tcttatacca ttgttcttgc ttcgagtcgc
    15661 agtatggagc cagttgagtt agttgaatct tatccagtca cggaagtttt catggaaggc
    15721 gcaactaacc agctagatca agaagtttta gatgatgact tggtcttgcc catcgaaaat
    15781 ggggagcttg accttgctga gagtgtatca gacaatatcc tgctaaacat tcctatcaag
    15841 gtcttgacgg ctgaagaaga agctggtcaa ggatttatct caggaaatga ctggcaaatc
    15901 atgacagagg aagaatacca agctcaaaaa gcagtaaaga aagaagaaaa cagtcctttt
    15961 gctggcttac aaggactatt tgacggagat gaataatgat cttgtcaaaa aaacgagcac
    16021 gaaaggtgct agaagaaatc attgccctct tcccagatgc caagcctagt cttgatttta
    16081 ccaatcattt tgaactcctg gttgcggtca tgttatcagc ccagacgaca gatgcagcgg
    16141 taaataaggc cacaccaggt ctctttgttg tctttccaac accccaagct atgtctgtag
    16201 cgacagagag tgagattgct tcacatattt ctcgcttggg attgtatcgg aataaagcta
    16261 aattccttaa aaaatgtgcc caacagttac tagacgattt tgatggtcaa gtccctcaga
    16321 cacgtgaaga attggagagt ttggcaggtg ttggtcgcaa gacagccaat gttgtcatga
    16381 gtgtaggatt tgggattcca gcctttgcag tggatactca tgtggagcgt atttgcaaac
    16441 accacgatat tgtcaaaaaa tcagcgacgc cacttgaggt ggaaaagcgg gtcatggata
    16501 tcttgccgcc tgagcagtgg ttagctgccc atcaggccat gatttatttt ggaagagcca
    16561 tttgccatcc aaaaaatcca gagtgtgacc agtacccaca attatatgat tttagcaatt
    16621 tgtaagatgc tacattgaaa ctttttgctt gattttaagc atgctttgtg ctatagtaaa
    16681 tgataactaa atattccttt aaacctgtcc cgtgaggcag gcaaggagcg cgataaagta
    16741 gaagggtgaa gtctatactg tgtgcagtag ggctcgcttg gctataaatt tcaagtctcc
    16801 ttgtttaagg agacttttct ttttggaggt ttgtcatgaa aacaaaagaa gttgtagacg
    16861 aattgaccgt caaacgagcg attacgcgta ttacttatga gattatcgaa cgcaacaaag
    16921 atttgaataa gatcgtcttg gctggtatta aaactcgtgg tgtctttatt gcccaccgaa
    16981 tccaagaacg tttgaagcag ttagaaaatc tttcagttcc tgttgtggaa ttggatacta
    17041 aacctttccg tgatgatgtt aaaagtggag aagatacttc tttggtttct gtcgatgtga
    17101 cagaccgcga agttatcttg gtggatgatg tgctctatac aggtcgtacc atccgtgctg
    17161 ctattgataa tattgtcggt catggtcgtc ctgcgcgtgt gagtttagca gttctagtcg
    17221 atcgtggaca tagagaattg ccaatccgtc cagattacgt tggaaaaaat atcccaacca
    17281 gtcgttctga agaaatcatc gtagagatga cagaacttga tgaccaagac agagttctga
    17341 ttactgaaga agcttagaaa gttaaaggag tagccatgtc agaaaatcaa caagcattga
    17401 accatgtggt gtccatggaa gacctcactg ttgatcaagt aatgaaattg atcaagcgag
    17461 gaattgagtt taaaaatgga gcccagattc cctatgaaga ccatccgatt gtttccaatc
    17521 tcttctttga ggattctaca cggacacata agtcctttga agtcgcagag attaaacttg
    17581 gattggaacg acttgacttt gatgtgaaga ctagttcggt taataagggt gagacacttt
    17641 atgacaccat tttgactctg tctgctttag gagtggatgt ctgtgtgatt cgccatccag
    17701 aggtcgacta ctatagagaa ttgattgcta gtccaacgat tacgacttcc atcatcaatg
    17761 gtggagatgg ttctggccaa caccctagcc agagcttgct tgatttgatg accatttatg
    17821 aggaatttgg ccactttgag ggtcttaaag ttgctattgc aggtgacttg gaccactcac
    17881 gcgttgccaa atccaatatg cagattttga aacgcttggg agctgaactc tttttctctg
    17941 gacctgagga atggagaagt caagagtttg cagactatgg acagtttgta accattgatg
    18001 aaatcattga tcaggttgat gtcatgatgt ttctccgtgt gcaacacgaa cgccatgata
    18061 gtggagctgt attttcaaaa gaagactacc atgcccaaca tggcttgact caagaacgtt
    18121 acgatcgttt gaaagaaaca gcaatcctca tgcacccagc tccaatcaat cgtgatgtag
    18181 aaatagcaga ccacttggtt gaagcaccaa aatcacggat tgtccaacaa atgaccaatg
    18241 gtgtctttgt tcgaatggca atcttagaat ccgtactagc gagtagaaac gccaactaaa
    18301 acacaagacg ttaaaagacg ccagtgagcg tccacgagag gtgaaaatat gacaaaaaga
    18361 attctagtat tagaagatgg cacagttttt gaaggcaagg ccttcggagc agatattgat
    18421 gtaacaggcg aaatcgtctt taatacaggg atgaccggct accaagaatc cattacagac
    18481 cagtcttata atggacaaat cttgaccttt acttatcctt tggtaggaaa ttatggtatt
    18541 aatcgtgatg attatgaatc cattattcca acttgtaagg gagtcgttgt ttttgaagaa
    18601 gcgcgtagag ctagcaactg gcgcaaccaa atgaccttgg atgaattttt gaaagccaag
    18661 aaaattccag ggatttcagg aattgatacg cgtgctctta ccaagattat ccgtaagcat
    18721 ggtactatgc gtgcaacctt gacccatgtt ggggacagta tggaccatgt gacggaccag
    18781 ctccaagcaa cagttttacc gacagacaat atcaaacagg tttctactaa aacgtcttat
    18841 ccagctccag gagttggttt gagcgtggtg ctagtggact ttggtctcaa gcactcaatc
    18901 ctacgtgaac tttctaaacg caactgtaac gtgacagttg ttccttattc gacaacggct
    18961 gaagaaattc tccatctcaa tcctgacgga gttatgttgt caaatggtcc aggtaaccca
    19021 gaagacgttc cacaagcact cgacatgatt cgcggtgtac aaggaaaaat tccaatcttt
    19081 ggtatttgta tgggacacca actctttgca atggcaaacg gggctaagac ctataagatg
    19141 aagtttggcc accgtggatt caaccatgcg gtacgtgaaa tcgcaacagg acgagtagac
    19201 ttcaccagcc agaaccatgg ttatgcagtc agccgcgagg atttgccaga gcacttgatt
    19261 attacccacg aagaaatcaa tgacaagtca gtggaaggtg tgcgccacag ataccaacct
    19321 gctttctctg ttcaatacca cccagatgca gctcctgggc cacacgacgc tagctactta
    19381 tttgacgagt ttatcgagat gatggaagtt tttaaacaat caaactaaga acgagtaaaa
    19441 gctcgtcgga gaatttatgt aactgaacac ggacggaaag ctcagaattt agagaaacca
    19501 acttggattg tcaatccttc cttgatttcc taaaaatcaa tcgctttcta ttcgtccttt
    19561 gtatcttatt taatgtcgcc ctgagaggct acgaatagaa aggagaaggg tgctccgtat
    19621 ttgctgaact gaatacgggc tacggacggt gctaaaaaga tagttattcc tagaactgac
    19681 agttcttcgt cataactcct attttagctt tgtccgcttg acgcccttaa tatcttatat
    19741 atgcctaaac gtactgatat tcaaaaaatt atggtgattg gttctggtcc gattattatt
    19801 ggtcaggctg ctgagtttga ctacgctggg acccaggctt gcttgtcgtt gaaagaggaa
    19861 ggttatgagg ttgttttggt taactcaaat cctgcaacca tcatgacgga caaggaaatt
    19921 gctgataagg tttacattga accgattaca cttgagtttg tgacacgtat tcttcgtaag
    19981 gaacgtccag atgccttgct accaacactc ggtggtcaga caggtctcaa catggccatg
    20041 gaattgtcta aaaatggtat cttagatgag cttagcgttg aacttctggg tactaaattg
    20101 tctgccattg accaagcgga ggaccgtgac ctctttaaac aattgatgga agagcttgag
    20161 caacccattc cagaatctga aattgttaac acagtagaag aagcagttgc ctttgcagcg
    20221 acaattggct acccagtcat cgttcgtcca gcctttaccc ttggtggtac tggtggtggt
    20281 atgtgtgcca acgagaaaga attgcgtgaa atcactgaaa atggtttgaa attgtcacct
    20341 gttacccaat gtttgattga gcgttcgatt gccggtttca aggaaattga atacgaagtg
    20401 atgcgtgact cagctgacaa tgccttggtt gtttgtaaca tggaaaactt tgacccagtt
    20461 gggattcaca caggggattc cattgtattt gcccctgcgc aaaccatgtc agactatgaa
    20521 aaccaaatgc ttcgtgatgc gagcttgagc attattcgtg ccctcaagat tgaaggtgga
    20581 tgtaatgttc agctagctct tgatcctaac agcttcaagt attatgttat cgaagtaaac
    20641 cctcgtgtat cacgttcgtc agcccttgct tctaaagcga caggatatcc gattgctaag
    20701 ttggctgcca agattgcagt aggtttgacc ttggatgagg ttataaaccc agttacaggt
    20761 tcaacctatg ccatgtttga accagccctt gactacgtag ttgctaagat tccacgtttc
    20821 ccatttgaca agtttgaaaa gggtgaacgc cgtctcggta cacaaatgaa ggcgactgga
    20881 gaagtcatgg caatcggtcg taacatcgag gaatctcttc tcaaggcctg ccgctccctt
    20941 gaaattgggg tgcaccacaa tgaaatacct gaacttgcag tagtttctga tgatgccttg
    21001 attgaaaagg ttgtcaaagc ccaagatgac cgtctcttct acgtatcaga agcaattcgt
    21061 cgtggctaca cacctgaaga aattgcagag cttacaaaaa ttgatatctt ctatcttgat
    21121 aaactcttgc atatctttga aattgagcaa gaattgggtg ctcatccaca agatctagaa
    21181 gttttgaaaa cagccaaact taatggtttt tcagaccgta agattgctga actctgggga
    21241 acgactgatg ataaagttcg ccaacttcgc ttggaaaaca agattgttcc agtttataag
    21301 atggtagaca catgtgcggc agagttcgac tccgaaacgc catatttcta ttcaacctat
    21361 ggttgggaaa atgaatctat cagatctgat aaagaatctg tacttgtcct aggttcaggt
    21421 ccaatccgta tcggtcaagg ggttgagttt gactacgcaa ccgttcactc tgttaaggct
    21481 atccaggcag ctggttatga agctatcatc atgaactcaa acccagagac cgtttcaaca
    21541 gacttctctg tatcagataa gctttacttt gagccattga cattcgaaga tgttatgaac
    21601 gtcattgact tggaacaacc aaagggagtt atcgttcagt tcggtggtca aacagccatc
    21661 aaccttgcgg agccattggc aaaagcaggt gtgaccatcc ttggtacaca ggttgctgac
    21721 ctagaccgtg ccgaagaccg tgacctcttc gagcaagctc tcaaagaatt ggatattcca
    21781 cagccaccag gacaaacggc taccaatgaa gaagaagcag ctcttgcagc tcgtaagatt
    21841 ggcttcccag ttcttgtccg cccatcttat gtacttggtg gacgtgccat ggaaatcgtt
    21901 gaaaacgaag aagatcttcg ttcttacatg cgaactgctg ttaaggctag tccagaccac
    21961 ccagttcttg tcgactctta tatcgttggg caagagtgcg aagttgatgc catttcagat
    22021 ggtaaaaatg tactcattcc tggtatcatg gagcatatcg aacgtgctgg tgtccactca
    22081 ggtgactcaa tggctgttta cccaccacaa accttgtcgc aaaaggttca ggagaccatc
    22141 gcagactaca ccaaacgctt agcaatcggt cttcactgcc ttggaatgat gaacatccag
    22201 tttgttatca aggatgaaaa agtctacgtt attgaggtca atccacgtgc cagccgtacc
    22261 gttccattcc tatctaaggt aaccaatatt cctatggctc aagtagcgac caagctcatt
    22321 cttggtcaaa gtttatcaga acttggctac caaaatggac tttaccctga aagcacccgt
    22381 gttcatatca aggcacctgt cttctccttt accaaactag ctaaggtaga cagcttgctc
    22441 ggtcctgaaa tgaagtcaac aggtgaagtt atgggttctg atgctacttt ggaaaaagct
    22501 ctctataaag cctttgaagc ttcttatcta cacttgccaa cttttggtaa tgtcgtcttt
    22561 accatcgctg atgatgccaa agaagaagcc ttgaacttgg ctcgtcgttt ccaaaacatt
    22621 ggttatggaa tccttgcgac agaagggaca gcagccttct ttgctagtca tggcttgcaa
    22681 gcccaacctg ttggtaagat tggtgatgac gataaggata tcccaagttt tgtacgcaaa
    22741 ggaagaattc aagctatcat taacacagta ggaacaaaac gaactgctga cgaagatggt
    22801 gagcaaattc gtcgttcagc cattgaacac ggggtgcccc tcttcacagc gctagataca
    22861 gctaatgcca tgcttaaggt acttgaaagt cgtagctttg ttacagaagc gatttaaata
    22921 gaaaaataag at
//
LOCUS       DAVKAO010000028        22010 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKAO010000028 DAVKAO010000000
VERSION     DAVKAO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.028"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(420..1109)
                     /gene="rplA"
                     /locus_tag="VWX16_001120"
     CDS             complement(420..1109)
                     /gene="rplA"
                     /locus_tag="VWX16_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9158488.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1317..1742)
                     /gene="rplK"
                     /locus_tag="VWX16_001121"
     CDS             complement(1317..1742)
                     /gene="rplK"
                     /locus_tag="VWX16_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9158489.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            1874..2011
                     /locus_tag="VWX16_001122"
     CDS             1874..2011
                     /locus_tag="VWX16_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158490.1"
                     /translation="MKIKEQTRKLATGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     K"
     gene            complement(2051..2767)
                     /locus_tag="VWX16_001123"
     CDS             complement(2051..2767)
                     /locus_tag="VWX16_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9158491.1"
                     /translation="MKKRYLVVTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(2848..3632)
                     /locus_tag="VWX16_001124"
                     /pseudo
     CDS             complement(2848..3632)
                     /locus_tag="VWX16_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(3652..4081)
                     /locus_tag="VWX16_001125"
                     /pseudo
     CDS             complement(3652..4081)
                     /locus_tag="VWX16_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
     gene            complement(4083..5120)
                     /locus_tag="VWX16_001126"
     CDS             complement(4083..5120)
                     /locus_tag="VWX16_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9158492.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDGQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFGFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            complement(5218..6543)
                     /gene="brnQ"
                     /locus_tag="VWX16_001127"
     CDS             complement(5218..6543)
                     /gene="brnQ"
                     /locus_tag="VWX16_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9158493.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6714..6806)
                     /locus_tag="VWX16_001128"
                     /pseudo
     CDS             complement(6714..6806)
                     /locus_tag="VWX16_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(6849..7001)
                     /locus_tag="VWX16_001129"
     CDS             complement(6849..7001)
                     /locus_tag="VWX16_001129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9158494.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(7154..7738)
                     /locus_tag="VWX16_001130"
     CDS             complement(7154..7738)
                     /locus_tag="VWX16_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9158495.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIYSQ"
     gene            complement(7792..9192)
                     /gene="pepV"
                     /locus_tag="VWX16_001131"
     CDS             complement(7792..9192)
                     /gene="pepV"
                     /locus_tag="VWX16_001131"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9158496.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFDFAGPAKEYLDITGKILLNDHEGESLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9205..9810)
                     /locus_tag="VWX16_001132"
     CDS             complement(9205..9810)
                     /locus_tag="VWX16_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9158497.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            9981..10781
                     /locus_tag="VWX16_001133"
     CDS             9981..10781
                     /locus_tag="VWX16_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9158498.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKISVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(10963..11796)
                     /locus_tag="VWX16_001134"
     CDS             complement(10963..11796)
                     /locus_tag="VWX16_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9158499.1"
                     /translation="MNHKIAILSDIHGNVTALEAVIADAKNQGVSEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEVLDGEYGLEYPKEIQSMRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLHFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQGHNLELLASLIEKHGYVEDVKDYFDF
                     L"
     gene            complement(11786..13630)
                     /gene="uvrC"
                     /locus_tag="VWX16_001135"
     CDS             complement(11786..13630)
                     /gene="uvrC"
                     /locus_tag="VWX16_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9158500.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVIFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13670..14371)
                     /locus_tag="VWX16_001136"
     CDS             complement(13670..14371)
                     /locus_tag="VWX16_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9158501.1"
                     /translation="MRWLFRLIGAFFFFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETNE"
     gene            complement(14450..15682)
                     /gene="murN"
                     /locus_tag="VWX16_001137"
     CDS             complement(14450..15682)
                     /gene="murN"
                     /locus_tag="VWX16_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9158502.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15687..16907)
                     /gene="murM"
                     /locus_tag="VWX16_001138"
     CDS             complement(15687..16907)
                     /gene="murM"
                     /locus_tag="VWX16_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9158503.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17019..17798)
                     /locus_tag="VWX16_001139"
     CDS             complement(17019..17798)
                     /locus_tag="VWX16_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9158504.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17867..19528)
                     /locus_tag="VWX16_001140"
     CDS             complement(17867..19528)
                     /locus_tag="VWX16_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9158505.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19703..21925)
                     /gene="recJ"
                     /locus_tag="VWX16_001141"
     CDS             complement(19703..21925)
                     /gene="recJ"
                     /locus_tag="VWX16_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9158506.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNTVLPEGVPVLDFPGELPNLAASEAVVVKNIPESITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
BASE COUNT         6235 a         5084 c         4061 g         6630 t
ORIGIN      
        1 agtagattga aactagaata gtacacctct acttctaaaa cattgttaga aatcgatttg
       61 actgtcctga acgattcgtc ctattcttat ttcattttac tataattgat agtggtcgcc
      121 ccagccagat accttatccg ctatataggc cttattatca cgttcctgtt cgtagagcca
      181 cgatttcgct atcccttcct ctgacgattc ttatttccag cgtcagactt aaaacggtct
      241 atccccagac cgttttaatc cgctacctca cgatagtcag acttgggaat cgctattggg
      301 ttcaccagta gtaggtgccc accgaggact cacacctcag atatacgaca tgcccgtcgt
      361 acatcaaaaa aagttgagac atagcgtctt caactttttt acattacttt aactactgat
      421 taaagtgagt ttacgtcaac tttgatacca acaccttgag tagttgtgat agtcaagttt
      481 gttacgtaag ttcctttagc tgtagctggt tttgcttttt ggattgtttc gttgaaagct
      541 ttaaagtttt caaccaattt ttcagcttca aatgatactt taccgatgat tgcttgaacg
      601 ttacctgcac ggtcagcacg gtaagtgatt ttaccacctt tagactcttc aactgcttta
      661 gcaacatcca ttgttacagt accagtttta gggtttggca tcaagttacg tggtccaagg
      721 acacgtccaa gacgtccaac aagagccatc atatcaggtg tagcgataac tacgtcgaag
      781 tccaaccaac cgtcgttgat tttagcaaca aggtcatctt caccaacaaa gtctgcacca
      841 gcagcttttg cttcttcagc ttttgcacca cgtgcgaaaa caagaacacg tgaagtttta
      901 ccagtaccgt ttggcaatac cattgctcca cggatttgtt ggtcagcttt tttaacgtcg
      961 atgttcaagt tgtaagcaac ttctacagtt gcgtcaaatt ttgcaaagtt agtttctttt
     1021 gcaagagcta cagcttcttc tacgctgtat gcttttgtgc tgtcgatttt ctcaagagca
     1081 gcacgaagtt gtttgctttt tttagccatt ttctattctc cttgtaagtg gttcaatcga
     1141 ttttcatctc ccacgtcact tctcgaaatg atgaagtctt gcgggttttc agtctactat
     1201 ttttctcctc ctacggcctt aagctccctt gctgtaccca agtacaagcc tcagtcactt
     1261 gcctagtatg aaaagcaact aatagactgc tgtgagattt tctactgtgt tattgattag
     1321 tcaacaacag tgaatcccat agaacgagca gtaccttcga tcatacgcat tgcagactct
     1381 acgtttgctg cgttcaaatc tggcatctta gtttctgcaa tttcttgtac ttgtgcacga
     1441 gtaactgtag caactttagt tttattaggt gtacctgatc ctttttcaac acctgcagct
     1501 tttttcaaaa gaacagcagc tggtggtgtt ttcgtaacga aagtaaatga tttatcttcg
     1561 taaactgaga taacaactgg aatgatcata ccagcttggt cagctgtacg agcgttgaac
     1621 tcttttgtga atcccatgat gttgatacca gcttgaccaa gagcaggtcc aaccggtgga
     1681 gctggtgtag ctttaccagc agggatttgc aattttacaa gtttttcgac ttttttagcc
     1741 atttttaaat cctcctttgt ggttttggcg gtaattagag atttttacct cccacaagta
     1801 tgtttttcac atacccatcc attatacact atttatctgg aattgcaaga gaaaagttta
     1861 ttttttatac tcaatgaaaa tcaaagagca aactaggaag ctagccacag gctgctcaaa
     1921 gtacagcttt gaggttgcag ataaaactga cgaagtcagc tcaaaacact gttttgaggt
     1981 tgtgaataga accgacgcag tcagtaagta aggcgacgct gacgtggttt gaagagattt
     2041 tcgaagagta ttaatcgacg tagtctccgc cttcaaagcc atagtattct tccaaactga
     2101 gaccacttgc agcaatttct ttagcttctt ttcctacata acgcaggtgc cattcctcag
     2161 ccatatagcc tgtttccttt tccttgcctt tgagataacg gacaacaaag ccataatcag
     2221 ctgcatgatc caagagccat tgggctgctt tttcttctgt caccaaatca ccatcagtcc
     2281 caatcacatc aaaggccaag cctgtctggt gttcgctata gccaggacgg gcagagtaac
     2341 ggtcagctgc tgcctttcca tcttggttga cataatcttg atagagcttg gtctgagttt
     2401 cataacttct aaaaccactg taatgatcac taatagggaa acctgcctct tgcatcgctt
     2461 tgatgagttt gaccaactca gccttggctg ttggattttc cccgggatta tagtctttcg
     2521 acaaaggata atgtttattg gctacgatga tttcgtcgta ttttccttgg atgctgtagt
     2581 aatcaccttt attgaccact tctgcttttt tctgggcagc cccttgagac ttactgccga
     2641 ctgtcccatc agctttggct gtctgttctg tcttagctgt gccatcttca ttttttgctt
     2701 tttcttgtga acaagctgct agactcaagg ctagcaaggc tgtcacgaca aggtatcttt
     2761 tcttcatttc tactccttta tttctaatag tttatctact tctgatgata aacgtttgac
     2821 taatctttta atagacttcc tgcaaaacta gaatcctagt tcatgattga taataccagc
     2881 aatcaaattc attcgtaatc cgaagcgttt acgatgattt cgataggttg ttgaaaacat
     2941 tttaaacgtt tttactttgg caaggatgtt ctcaaccttg cttctatcct tagatagcgc
     3001 atggttatag gctttatctt cagctattag cggcttgagt ttgctggatt tacgtggagt
     3061 ttgtgcttga ggatatatct tcatgagccc ttgataatca ctgtcagcca agattttacc
     3121 agcttgtccg atatttctgc aactcatttt gaacaacttc atatcatggc aatagaaaca
     3181 attctccctt gacttgtgac aatcgcttga gccttcatag cgtgaaattt ctttttacca
     3241 gaatgattcg ctaattgttt tttagggcga ttgatttgta cttccgtcgc atcaatcatt
     3301 accgtgtcct cagaactgag aggagttctt gaaatcgtaa caccactttg aacaagagtt
     3361 acttcaaccc atcggctccg acggattaag ttgctttcgt ggataccaaa atcagccgca
     3421 atttcttcat aagttcgata ttctcgcaca tattgaagag tggccataag aaggtcttct
     3481 aggcttaatt taggttttcg tccacctttt gcgtgtttaa gttgataagc tgtttttaat
     3541 acagctaaca tctcttcaaa agtcgtgcgt tgaacaccaa caagacgctt aaatcgtgca
     3601 tcggttagtt gtttacttgc ttcatcattc atagaactac tataccatat tttatttcgc
     3661 aggaagtctg atctcatccg gttttgcttg acgggtttct gcggttattt cttcaaaagg
     3721 gacccaccag actggcccac agcccttgag accgtggcca ttcatatcac ctcttcgtct
     3781 agggaaaagg tgccagtgga gatgggcatc gccatttcct agaagttcaa tattcatttt
     3841 ctcagcagta aaggccttgg caactgcctc ttggactaaa ctcatttctt ctagaaaatg
     3901 gagtcttgtg tccttttcta aaatggtgca attccgtgac atgttccttg gctaaaaaga
     3961 gactatagct tgcaaaatac tgatggtctc caatcacaag atagcctgtt tccaactctt
     4021 tgacaaagta gggattttct cccttcttga tgagctcaat tctctggcaa ataaggcaca
     4081 tatcaatcta ctaaattgct tctaagatag gcatcgacca ttcgttctgt tgcgaccacc
     4141 gagtcaatat gagtgcgctc ataagaatga ctagactcga tacccgcacc aagaagggca
     4201 tgcttgacct ctgcccctgc agacatagcc gctgaagcat ccgaaccata aaatggatag
     4261 atgtccagct taaaaggaat atcttgctct ttagccaagg caaccaagtg ttgacggaag
     4321 tcatagtgat aaggacctga agcatccttg acacagatag atactgtgta ctcatctgtc
     4381 tgctggtcat ctcccatagc tcccatatcc acagccagat attctactac ctgagcagga
     4441 atgttagaat tagcaccatg tcctacttct tcaaaaactg aaaaaccaaa atgagttgtt
     4501 actggcaatt caatcttctc tttcttataa atacgaagca ggttgagcaa aatcgctgcg
     4561 ctgaccttat catccaaatg acgagacttg ataaaacctg tatcagtcac aacagttcgt
     4621 ggatcgaagc tgataaaatc accgacctca atcccaagag cacgagtttc tttttcattg
     4681 cttactttta catccaaacg cacttccata ttgtcctgcg tgcgttctgc agttcctgca
     4741 tccttgtaga catggcaaga agtttggtgg atgaggatgg ttcctgatac cttttgacct
     4801 gtgctagcca catgaacggt acagttttca ccttcaatca tgttccaagg aaaaccaccg
     4861 atacggtcca atttaagacg accatctggt ttgacagcac gaacaatagc acccagcgtg
     4921 tccacatggg cagtcacata gcgatgttgc ccatcatttt gacctttgat ggtcacattg
     4981 acaccaccct tggctgtgcg aactggctgg taaccaaaac cttctagagt cttgactaaa
     5041 taattcgaaa tttcacgagt gaagcccgtt ggcgatgcga tggctgtcag ttctttgata
     5101 tattctactg tttgattcat ttcttcacct ctttcgctac ctattataac acatttatca
     5161 tcggataaaa aaagaaaatc actcctgtag acttggctac aaaagtgatt taagtgatta
     5221 ttccatttca aaaacatcgc tttcttgctt gtttggtaga accaatgaga gcaagattcc
     5281 aacaatggct ggcaccaacc atgggagaga tgccttggca aaaggaagaa cgctaacaag
     5341 atttgcaaga aactcaacct taaacgagct tcctagtacg cttgcaatgg caataactgt
     5401 aaccacagca attgtcaact gcatacctgg ttttgaaagg gcaacaaatt tgttgacaat
     5461 gacaatcata acgatagcaa tcgtgattgg gtacaagata accagtactg gaattgagta
     5521 cttgataatc gcatcaagac ccaaattggc aatagcaaat ccaatcaagg taaaggctgt
     5581 cgcataaacc ttgtagctga tttgtgggaa gcgctcatta aagaactcag ctgttgacac
     5641 aatcaaacca acagtcgttg tgaagcaggt tacggtaacc atagctgcaa ggaagagttg
     5701 agctgttgag ccaaagattt cttgagtggc ttgtgacaag atgtaaacac ctggtgttcc
     5761 acccttcatc gcttcagctg gtactgggaa atgatttcca agaaaaccta aaccgatgta
     5821 aagagcgctg aaggcaaggg caacaacgat accaacaacc caaatagttg aaatgtattc
     5881 tttcttactt gaaaatccaa gttgtttcaa ggtttgaact gcgattacgc taaaggccac
     5941 tgaggcaagg gcgtccaagg tattgtaacc ttctaggaaa cctgtaccaa aggcagaagc
     6001 ttgataagca gctgaagcag cttgaggact tgttccacca tatttgatag ctcccagaac
     6061 gaccaagata acaatcaaaa ttgcaaagac tggcgttaaa atacgtccaa tgcggtctaa
     6121 gatttttgat ggattaagcg aaatcaaata ggctgccgca aaatacagaa ccgtaaatac
     6181 aatcaagcca agtcctttat ttgcatccga caaaaggggg ctaatcccta cttcgtaagc
     6241 tgttgtagca gtacgtgggg tagcaaagaa tggaccgatt gacaagtaaa gaactgagag
     6301 gtaaagagtc gcaaaccaag gcgctatctt cgttgaaatc tcgtagatat atcctttagg
     6361 atttagcgtt ccaataataa gggtcaagac ggcgatacca acgcctgaaa agacaaaacc
     6421 tgcgatggca ggaagaaaat gttctccaga tagagcacct agagaaggcg gaaaaatcaa
     6481 gttccccgca ccaaaaaata ttccaaacag gagcaaacct gttagggtac cttttttagc
     6541 cataaaaatc tccttcatat ttataaaata ctgacctagt ataccacgat taggagtctg
     6601 aaaaaagcat cacgaggcaa atattaaatg ttttcaattt tcttaaaaat aagaattatc
     6661 taaaaataaa actctcctac ccagtccaca ccaagtagga gaaatgcaaa attttaaagt
     6721 tcttcaaagg ctaacatgcc accctggaca ttgataacat tataaccttg ttctaacagg
     6781 aattagcaag cacgcgccga tctcattcca gatttgcaaa taatataatg caagcgatct
     6841 ttgtccaatt aatcataact atcagccaat tgactaagcg gtaggttgtg ggcaccttct
     6901 aaatgaagag ctgcaaactc atccacttct ctcacatcca ctagagaaag ctggtcgttt
     6961 tggtaaagct ggtaaaatgc gtcaaaggct atttctttca ttctttttct tctaatactc
     7021 ttcgaaaatc tcttcaaacc acgtcagcgt cgccttaccg tgcgtatgtt accgacttcg
     7081 tcagttttat ctgcaacctc aaagctgtac tttgagcagc ctacgactag cttcctagtt
     7141 tgctctttga ttttcattga ctataaatgg ttttaattct ttttttcaaa tctggcacta
     7201 cttctgcctc aaaccaagga tttttggcca tccagatttg atttcgtggt gatgggtgaa
     7261 ctagcggaaa ataggctggc agatagtctt tatagtgttt caccctctcc gttaccttcc
     7321 cactgatttt ctcctgtaaa tagtaggctt gggcatattg cccaatcaag agggttaact
     7381 gaatatcagg caattcctgt aagagctgcg gatgccattt ttctgcaaaa cctgtacgag
     7441 gcggaagatc acccgacttg ccatgtcctg gaaagtagaa atccataggc aaaacagcaa
     7501 aataacctga attgtaaaag gtatcttcat ccacacctag ccagtcccgc aagcggtcac
     7561 cacttttatc tttccagtaa aggcctgctt cttgagtttt aagtcccgga gcctgaccga
     7621 tgatattgat gcgagcagtt tttggcgctg caaagagagg ctcaatgcca cgctctgtat
     7681 agctggcatt ctgtgaatcc gccatgatag cctgtttgat tctttcgatt tgagacatct
     7741 actttccctc caaaaagaag tccaagcata agatctcaga cttctatcgt tttatttgat
     7801 caattcgtaa atagcttcgg cataaattgc tgctgctcgg aagagatcat ccaaggcaat
     7861 aaattcattg gcttggtgca tggtatcaat tgagtctggg aacatagcac cataggcaac
     7921 tccgcgctct aacaagcgac caaaggttcc accaccgatg acttgttcat gacctttaag
     7981 gcctgtttgt ttttcataga cattcaacaa ggtttgcaca agtggatctt ccattggcac
     8041 atagtgaggc gtgtgaccgt gttcagacag gctaacagaa acaactggca agttttcaag
     8101 gattgacttg atttgttctg gacttgttcc ttttggatag cggatgttga gggcaatggt
     8161 attatcagca cttgtttcat cgaagcggaa gacgcctgca ttcatagaaa gggcacccat
     8221 cttttcatcc acatgagcaa tcttgagact ttcaccctca tggtcgttca agagaatttt
     8281 accagtgatg tcaaggtatt ctttggctgg accagcaaag tcaaactggc taaggaagag
     8341 ggctaggtaa gtcgcaccat tgacacctga agcaggcata gcaccgtggg ctgatttacc
     8401 aatgatcgtc accttgtatt tgccagcttc ttcttggagt tctcctctaa gtttgtgttc
     8461 tgcaacaaag gcatctagtt tagcttgcaa gtcagccaaa tcacctgaaa cgactgctgt
     8521 tgctgattct ggtaccatat tttcacgtaa accacctgta aagctgtgaa gacgggcagc
     8581 acctgtattt tctcctgcaa agtggaggta ttccgtgata tttccttttt caccattgat
     8641 gatagggaat tcagcgtctg gagagaaacc gaaatctggt ttggcaagtc ctacgtgctc
     8701 aaagtagtag tccatatctg cccagcctga ttcttcgtct gttccaacga tgaagcgaac
     8761 tttcttagaa gttggaagac ccaattcttt gatgattttc aaaccatagt aacaagctgt
     8821 tgtaggaccc ttatcgtccg aagccccgcg cgcataaagg cgaccatctt tgatagttgg
     8881 tgtgtaagga tctgtatccc aaccgctacc agctggaacc acgtccatat gggcaaagat
     8941 tccgagaact tcttctccat caccaaactc aaaatgtcct gcatagttat caacattttt
     9001 agttggataa ccatcacggt ctgcgatttc aaggaatttt tccaaggctt ttactggacc
     9061 aggtccaaat ggatgttggg catcagcctt gctatcatca cgttctgagt tgatttccaa
     9121 aaggctaaac aagtcagcca agaggtcttc tttgcgtttt tctacttctg ctgtaaaatc
     9181 aattgctatc attttttctt cttcctatct tttttcgatg atttcatcta ctggcaagcg
     9241 gtagcttggt tccaatttgt cgtctgtgta acccactgtg atcaagagtt ctgggcggaa
     9301 acggtcttcg atttccaaaa cttcattaac ttttgatttg tcaaaaccaa ggataatatt
     9361 ggaaccaatt ccttggtctg taagagcgag aaccaagttc atagcaacca aacctgcatt
     9421 gagtgctagg tagtcgctga cttgttgttc attgtagcgt gcaaattcag ctggcaaatt
     9481 tttcatgaag tattgaagtt gttcttctga gaaattgtta gcaccaccaa ctcgggcaat
     9541 cttacgagcg cgtttggcaa ggtctgtatc tgtaaatagg gcaatggtta caggtgctga
     9601 tgatacctgt tcaaaattgg aaccataagc taactttgcc agttcagcat ttttctcacg
     9661 taccaccaca aatttccaag gctggctgtt gtgggcgctt ggcgccaagg ttgcaatttc
     9721 gatagccgta cgcacatctt tgggatcaac aggcttatca gtaaaatgct tggtcgcatg
     9781 acgtttttta tttaattcaa gaaatttcat aatcgatttc cttttctaat tctactgctt
     9841 ccattctacc ataatttgaa agagcttgca gggatttttc atgattaagt ttttctatca
     9901 cagacataaa agatatatat tggtttatca ggaaagtttc tgataaacta gcaagtactt
     9961 tacatttgaa tggagacagt atgaaaaaat ttagcctatt actagctatc ctaccatttt
    10021 tggttgcctg tgggaatcaa gctacaccca aagagactag cgctcaaaag acaatcgtcc
    10081 ttgctacagc tggcgacgtg ccaccatttg actacgaaga taaaggcaat ctgacaggct
    10141 tcgatatcga agttctaaag gcagtagatg aaaaactcag cgactacgag attcaatttc
    10201 aaagaaccgc ctgggaaagt atcttcccag gacttgattc tggtcactat caggctgcgg
    10261 ccaataattt gagttacaca aaagagcgtg ctgaaaaata cctttactcg cttccgattt
    10321 ccaacaatcc cctcgtcctt gtcagcaaca agaaaaatcc tttgacttct cttgaccaga
    10381 tcgctggtaa aacaacacaa gaggataccg gaacttctaa cgctcaattc atcaataact
    10441 ggaatcagaa acacactgat aatcccgcta caattgattt ttctggtgag gatattggta
    10501 aacgaatcct agaccttgct aacggagagt ttgatttcct cgtttttgac aagatatccg
    10561 ttcaaaagat tatcaaggac cgtggtttag acctctcagt cgttgattta ccttctgcag
    10621 atagccccag caattatatc attttctcaa gcgaccaaaa agagtttaaa gagcaatttg
    10681 ataaagcgct caaagaactc tatcaagacg gaacccttga aaaactcagc aatacctatc
    10741 taggtggttc ttacctccca gatcaatctc agttacaata atactcttcg aaaatctctt
    10801 caaaccacgt cagctttatc tgcaacctca aagctgtgct ttgagcaacc tacggctagc
    10861 ttcctagttt gctctttgat tttcattgag tataagatat actaaaagcg attggaccag
    10921 ccaatcgctt ttttagtttg cattggttat tttaggaaac tcttacaaaa aatcaaaata
    10981 atctttcaca tcctctacat acccatgctt ttctattaag cttgctaaaa gttccagatt
    11041 gtgtccctga tagttatctt cacgacgtag ttcttcatac atttcgataa agggaagccc
    11101 cttggacttg gccaattcta actcagcctc gtaatcataa gcaactttac gaaattggat
    11161 attgaccaat tccccgtctt caacttctat cacggcatac tgggcacggt gattttttaa
    11221 cgcctcccaa ttaaaatagg gcatgccaat cgaccctgga ttgatgattt gttgcccttg
    11281 acttccataa cgaagcaact gcttgtgaac atgaccataa actgccacgt ccgtttccgc
    11341 atctagcagt tggtcaaatt tctctgtatc attctcaact agcaagtcac caccatagtt
    11401 tttgtcaggt aaattatgag agatagaaaa gtgcaatccg tcaatttctt tcttttccag
    11461 caaaggcaag cttcgtagcc agacaatcgt tgcaggatcc attcgctcca tcaaaaactg
    11521 ggtcatgcgc attgactgga tttctttcgg atattccaaa ccatattcgc catccaagac
    11581 ctcaaggaca cgatcatccc aattgcctcg aacacttgct gtgataggaa ggtcctttag
    11641 cagggcgact aagtcatttg cgcctggacc aggaagaaaa atatctccca gaagccagta
    11701 ttcactgact ccttgatttt tagcatctgc aatcactgct tctagcgccg tcacattgcc
    11761 atgaatatct gataaaattg cgattttatg gttcattgtg gtttccttcc gtttggtaat
    11821 ctactctttc ttctgctact tgaggcaagg cttctccctg cgggttcaac tttctttgca
    11881 cagcctctgc aacgactcta ggtaccccaa cttcgacaat ctcatccaca ctggcttcct
    11941 tgattttggt caaagacttg aaatgcttca taagattctg cttgcgttta ggtcccagac
    12001 cgtcaatccc atccaattga gatgagaaag aatttttgga gcgcagttgg cggtggaaag
    12061 tgatagcaaa gcggtgcacc tcatcttgga tgcgttggag gaggaaaaat tcctgagaat
    12121 tgcgagacaa gtccaccacc tcaagcggat ctccaaagag caattcatgg gtttggtgct
    12181 tatcattctt ttgcagccca gcaattggaa tatccaagcc cagttcctct tggataacct
    12241 gcttagcgat attgacttga ccttgccccc catcaatcac aatcaaatct ggaggagtca
    12301 aagcctcacg ctgtactcga ccatagcgtc tgcgaatgac ctctctcatg ctggcatagt
    12361 cgtctggtcc aacaaccgtt tttatcttgt acttacggta atccttctta ctcggtttac
    12421 cgttgacaaa gatcaccata gccgaaacag ggctagttcc catgatatta gagttatcga
    12481 aggactcgat acgtactggg gtcgggattt ggagcaaacg ccctagattt tcaatagctc
    12541 cttgagtctt ttcgacagat ttttctagca ggttaaattt ctgctccaga ctaacacgtg
    12601 cattttttat ggctagattg accagttgtt ttttctctcc acgttgaggc ttaagaatct
    12661 tggaatccac caaggccttg acagcctctt cgtcaatatc ctgcggaatc agtacctcat
    12721 tgggaactag atgagatttt tcttgataga attgtcctac ataggtcaaa aaatcctcat
    12781 ctggatcatt gaagtagggg aagagattga catcgcgctc gataagctta ccctgacgga
    12841 caaagaaaac ctgcacacac atccagccct tatccacata gtagccaaag acatcgcgat
    12901 tttgcaaatc tttcgccatg acccgttgct tggttcgaag cgttccaata gcctgaatca
    12961 ggtcacggta ttccgccgca cgttcaaact ccatactttg tgctgctact gccattttac
    13021 tcttgagatc atcgatgatt ttgttatcct gccctttcag aaaatcagaa acctcctggg
    13081 ccatagactt gaaataagtc tcatccttct tacagatggt gtgggccatg cattgaccga
    13141 tatggtaata aaaacagacc ttagagggcg ggttggtaca cttacgaaaa gggaatatcc
    13201 gatccagcaa ccgcttgatt tcattggctg cccccacatc gggatagggt ccaaaataaa
    13261 gacctccgtc ctttttgacc tgacgagtga taatcaagcg tggatagcgc tcattggtga
    13321 ttttgatgaa aggataggac ttgtcatcct tgagcatgat attgtacttg ggcttgtttt
    13381 ccttgatcag gttgatttct aggagaagtg cctcaatatt agactccgta acaataaatt
    13441 caaaatccac aatttcagac accagagcct ctgtcttggt atcatgactt ccacgaaaat
    13501 aggaccgtac tcgattacgc agatttttag cctttcctac atagataatg gtgccatttt
    13561 tatccttatg aatgtagcaa ccagggctgg tcggcaagag ctctagtttt gatttaatca
    13621 agttgttcat agttccatta tagcaaaaaa agtagggaaa gatagtaccc tactcattag
    13681 tctcatatat ttttcggagg tttgcaacat cctcttcctg gataccataa aaggaacctg
    13741 ctggttgcat gacagacttc tcatctgtat ggtccaagcc aatcgcatga cctaactcat
    13801 gttctgccgt atggacaagg cgttcatagg aatagccata gtatggattg gacaaataat
    13861 aatgattcaa ccgcaccgtt acggacaaga attgccctgt taagagatta gtttgacttt
    13921 ccgcctctcc tgccacagga gtgcctccgt cgttcatctc cgtagccgta atatccgcct
    13981 tactagactc agtcacgagt tcaaagttaa aagcaccagt ttgattccag ttctggattg
    14041 cttctaaata agcctcttga aagcgtgaat ccatctgcgg atccaggtaa atacgagcag
    14101 aggcctgtgg ccaccttcct tcagaatgct ggtgactatc tgtctggctt aacttaggca
    14161 gttgccctgt tttctcccat tggtcaatac tttgttgacc aatttttact gaccgttctg
    14221 cttgctttag cgctccttga aaatccccgt tcaaatacca gagaagtccg aaagcaagca
    14281 cacataagag cacaactatc caaaccagac gccaaaacaa acgccacaca aaaaaaaaga
    14341 aagcccctat caaacgaaaa agccagcgca tgtctctcca cctttctagc aaataaaaat
    14401 tattttacta aaagaagcta aaagaaatct aaatactgac tttttcatct taacgtccta
    14461 ctattttttt gagtaactgg atagctttgt attttaaagg cgatggatgg taacggaaag
    14521 tacccgcttt gcgtacaata tagccattaa aattctgttt aaaacgcaaa acaccatcac
    14581 ttccatcaaa aatcccttga atgcctagga aattgtattt aggtattcca cgttttatgc
    14641 tttccaacat aacatatttt tgaagcagtg caggggcata gaacttatta aactcagtgt
    14701 aggaaccact aaagagataa gtcgtttcct gaggcatata aacaaataaa ctcccagcta
    14761 aaacaatatc ttcttctcca tatttttcaa tcaagtctcg cgcttctgct tttcgaactt
    14821 caaacgtttc aaattgacta gaatattctc tcagttgatt ttgttttttc tcagaatgag
    14881 gatttttact caaatcaagt cgcaacttgt ccaagttttc ttctagttta ctttgttcac
    14941 cttgcaattt gctcatatac tccgaaaaat tcaagcttgc tatgagaaac tccgcttgtt
    15001 ctccaaaagt atcataaaaa tgctcataat attctaaact tttatcacta tattctctac
    15061 gttcagaggt ttcttttgtt atattcttaa aaatcgatag ttcttcacgt tttaactttt
    15121 tcaaccgaat gccaaaggtt tcagcctttt tcaccaaggg tttacccttt ttgctaaaac
    15181 ttttaagcaa actcttttca gttaattcag ttaaatcttt atagtataac caatctggtt
    15241 ctccacctgg gtaacctgtt gttaagccat caaattgata acctaaatca gtcaaacctt
    15301 gaataatact ttttttctca gcatctattg gattaccttg gctatcaaaa gtttgataag
    15361 tttcataagg ttttacaagc aactctaata caccattttg cttggcatat tcttttaact
    15421 ctgcataaaa aactggaaga gcatcttgtt gggtataaat cggccccgaa ttgagttcca
    15481 tatgcagacc acccagcatg ggcaagctat aaaccagagc tgcaacttga atttctcctt
    15541 cttgtttcaa agcaagataa acaattcgag cccctctttt ttctagcaaa tcccccatct
    15601 ggacagattg cataaaggaa cgagaagaaa cctgatcaga ataagtctga aactcttctt
    15661 tcgtgagtgt tgttagtgcc atatacttac tttctatgtt tttttcttaa tgttttacgg
    15721 aaatcaagag caagtcttaa cagaggatag agaggatgag tgggcattgt aaattcaccc
    15781 aagtattctt caatcgttgg attaaatttt tccttaaaat gataaagtcc accattgaga
    15841 gagttttcaa caccacctaa attttgccag accatacctc gctcaaaggc atagcgagcc
    15901 gtttcatacc atgttaaaat tggtgcattg taacgtttaa aatcatcatc cataccagca
    15961 tatatattga cagaggtagt accaaattcc aaactcaaag tagccgctaa aggaactctc
    16021 gcttgaccta catctatata ttcctgcaag aaggtcaatt cctctaacaa acgttctttt
    16081 tccttcttct gcgcttctac ttttgaaatt cgagtcgact cagtaaacgt ctcttccaag
    16141 gctctatttt tcgctaactg ttcttctaat tcttgcgaac gtttagaaac atccaaggtg
    16201 gccaaggtga tataggcctt gtccttaaaa ttatctaaca attttttata ataggcttca
    16261 ttcctcaaat gaatctcttt tcgcttctca gtttttttca tcaactccga aaatgaatct
    16321 aatagttcca gtccaccata ttgaatctca agccctttgt ttcgtgctgt tcgaatagcc
    16381 tgttttgttg acttggaaag tttatcttct tcaaaatttt ccttgtatat tttcgcctga
    16441 atacgaggtt gaatggtgtc tcccatttcc tccgtttttc ctgaccacct tactcccatt
    16501 tgttgcaaac tatcaataat agccagattt tcaggaaatt ctgtcttttc ctgattgatt
    16561 aaactttgag ataggcaaat acttgggtca aaagtcacaa aaaccgctct cttactgcga
    16621 gcataggact taatagactg aatggcaaaa ttcaagagtt ctttatcccc ataatccaat
    16681 ataggtcctc ttgggatgta aaacatttta tagcctagcg gaagagttct aatcaaaata
    16741 ctagctgtcg ccagtaattt ttcttccctg taaacaccaa acttctcatg ttgccaatta
    16801 gacttaactt cctcccaagc actactttgt aatacattgg ctaattcatg ttctttgaca
    16861 aactgatcat attctaatgt gggaatgcca atttgataac ggtacatttc ctactctctt
    16921 tcctccagta ttttattact attctactac tttcgtttga aaagtctagt gaaaacatat
    16981 tttattttag ttctactccc cctatgctga agttcaaact aagtcagtct ctcttctaat
    17041 ttgaaatcaa ttggtagggt tgttaaaaaa acttccaatt gtttttccca gtagtaccac
    17101 tcgtgagttc cagcactatg gctataggtc acatcaaaac ctagtttttt gagatttttc
    17161 actgcgagat tattggcttc gtacaagaaa tcctgttcgc cacaccacgc ccaaagtttg
    17221 gtctttttat ccgatttttt agccagactt tcaagagaat agggactagt tgtccagtct
    17281 ctaatctctc caaaaacacc tctccagtag gctggacttc ccagattttg actttcagga
    17341 gaaaagtttt gaaagctgag ggcacctgaa aaactagctg catgagaaaa acgatttgtc
    17401 gtaagagcca gtttgaagca gccgtagcct cccatagaaa gaccagcgat aaaggtcttt
    17461 tcacgcttgc tagtcatatt agggaagaag cgtttcagaa cctgtggcaa ttcctctgct
    17521 agagccgtgt agtagtcaaa accatactgg gtgtcggtgt accaaccatt gctggtattg
    17581 ggcataacaa cgatgagatt agttcctcga agcaagcgtt ctacattggt ccgcttaagc
    17641 caactattat gatttccaga catcccgtgc aaaaggtaca agacgggaat atcttcacac
    17701 tctggttctt ccactcgatt ggcatcaggg tagaggacat tcacccccca ctccatatcc
    17761 aatacttgtg agtaatactc gattttcatt actgccatga ttttctcctt ctatttttct
    17821 ataagaaaaa gccgaaactc gactttctct ctgtttattt caacgattat tttgcttcca
    17881 tgactactgg taaaatagct ggacgacgct tggtttggtc aaagagatac ttggtcaaat
    17941 tatctcgaac cttcccttta agatctgccc agtcaaagtc atctccttga agataatctt
    18001 ctaccgtttg gttaatcaat tctgaacttt cacggagaat atcgcgactc ttcttgagat
    18061 aaacaaatcc gcgcgtatga acacgagccc tagccacaat tttcttctca cgacggttga
    18121 ctgtaatagc cacgatgaaa attccatcct ctgacaagac cttacggtca cgaagaacaa
    18181 catttccaac atcaccaatg gcattcccat caattaagat atctcctgct gaaaccgatc
    18241 cagctggaac aaagtctcca ttctcgtaag ccatggtcgt ccccttttta ggaatgaaga
    18301 tgcgttctgg caacatccca actgccatgg cagccttagc gtgagcatcc aactcacgat
    18361 actccccttg gacagggaag aggtacttag gttgcaaaag attgatcatc agctgcaaat
    18421 cacgcacatt tccgtgccct gatacatgta aactttgggt aatcaatttg acaaccccac
    18481 ctgcctgata aatcatattt tccacacgcg caacaaaggc ttctttagca atagacggag
    18541 ccgtagcaat atagaccagg tccccatcct tgatttctac ataacgatgg cgaccaatcg
    18601 acatcttacg aagtccattg ataggttcac ccatacgacc tgtctcaaga ataatcaact
    18661 catggtcttc aaagcgagac atatctttag gcttaatcaa gagaatttcg ttggctaaag
    18721 acaacttctt aagacgaatc gctgtgcgga cgatattttc aatatcaaat cctgtcaaga
    18781 cgatacgtcg acctgtttta tccgcagcgt caaaaatctg ctgaatacga gaaagattac
    18841 tggaaacagc tgcaacgatg atacgacctt cccagtcagc aatagtttgg gtaatttcat
    18901 ccctaacttc actttcacta gccacctgaa tattgctgtc tgcattggcc gaatcactga
    18961 ggagagccag aacgccgtca cgaccaatct ctgccaaacg agcaaaatca gttgcataag
    19021 attcactagc cgtttggtca aatttgaagt cacctgtata aacgatgctt ccttccgatg
    19081 tcttcaagac aattcccaga ctctctggaa cggagtaagt cgtagggaag aaggaaacca
    19141 ctgtcccacc aaaatcaatc tccgtattct catcaatgac atggaaatca ttaaatttct
    19201 taacggcatc atttcctttg acaaagagct ttgccaactc aatggtcaac tcagacccaa
    19261 atacaggaac tttagcctct gccaagagat aaggtagagc accaatggca tccgcatgcc
    19321 cgtgggtcaa gaaaacccca gcaatacggt cgctattttc aaaaaggtaa tccatgtttg
    19381 gaatcaccac atcgacccct aattgttcat tttcaggata ttttaaccct acattcaaaa
    19441 caaaaatgga ctctccaatt tcagcaatgt acatattttt tccattctca cgcacaccac
    19501 caagtgttgt taaactgata ttactcattt ttcctccaaa atcttagttt atcttgatta
    19561 tagggcaact ctaccctctt attctaaaag ttctaatact ttcagccgaa tcttttccta
    19621 ccattatacc atttttttga tgattttcaa aatgaaggtc tgttttcaaa aaagagttga
    19681 tttcccaact ctttcctaac ttctactctt tttccatcaa aaaatcataa atttcttgca
    19741 ccgtacccag cgccatcatt tcttggtctt taacggtttg tttgagattt tggtaaattt
    19801 gactttctcc tatctcccgc tttggcgcct ctttattgac tgtcatcaca ccatctttta
    19861 tcgtcacaaa gcctagttct tcaaatactt gaatcatctt gaccagcaag atttgttgaa
    19921 tattaagata tgcagccaaa tctttcagct tgtagcgaat atcaaactct gggaactggt
    19981 aaatagtctt gtacaatttg gcaaactgat ctctagtccc ataacctgtc agataatagg
    20041 ccttgtcaat atcatttttg aaatagacag cagagaaatg ctgttcctga aaaatggtct
    20101 tcagctgagt aatactctct ggaatgtttt ttacgacaac agcttcacta gccgcaagat
    20161 ttggcagttc tccaggaaaa tccaagactg gaacaccttc tggcaagact gtattttttc
    20221 cacgaatgtt aaaaagttga acaccttcca ctcgcgcatc caccatcatc aactggaggg
    20281 cagtttggcc attccattgg ttgacagaca atttaaccgc taactctaga ttcttggttt
    20341 gagaaaactc tgtcgcccat ctgccttgac caaaggctac cacttcaaaa ctcgcctcac
    20401 ccttggaaat tttcagcttt agatgggcat tacctgcccc catagtacga gcactttcga
    20461 cctgaaaatt cttgatataa aaaataggtt tctgattatc cattccaaaa ggagctaaac
    20521 gttcaaaact tttgaccgtt tccaagctaa gtgcctccaa atccaactct tcatctaggt
    20581 ttaacttatt ctttccacca gcatctgcac ctttttcacg aacataatct tccaaaacct
    20641 gagataaatc tgagagttgc tcaacttcca gcgtcatacc cgctgcacct gcatgacctc
    20701 caaaggcgat gaagaggtct cgatggggat ccagagcttc aaaaatatcg accgcttcca
    20761 cactacgagc actgcccttg gcacgaccgt cttctatatt aagaacaatg actgtctgtc
    20821 ccaattcttc caataaacga ccagccacga ttcctagaac cccaggattc cagccttcct
    20881 tggccaagac ctgaaccttc ttctcaggat ccaccatggt cttggcttct tcatagatag
    20941 actgaacgat ttccttgcgc tcttcgtttt tctggtgaat cataagggca atctcatgcg
    21001 cttcctcatc atcaaatcca gtcaacaaat caatggcagg attgggatca tccaagcggc
    21061 ccaaggcatt caaacgagga gcaatctgga aaccaaccgt ttcttctgtt acttcgttgg
    21121 cagcaatccc agccatgtcc agcatttctt gcagaccaat gcgctgggta tgacccaaca
    21181 tttccagacc atattgaact aagatacgat tttcatccgt cagactcacc atatctgcaa
    21241 tagttccaat agcgaccaaa tcaagcaatt ccacttgcac ttcttctaac agggcacaag
    21301 ccaacttgaa agcaactcca caaccagcca aatatttaaa aggataatcc gcatctggat
    21361 gttcaggatg gacaatagca taagcatctg gcagggtttc aggcatggaa tgatggtctg
    21421 tcacaatgac atctactccc atagactgag ccaatgcaat agcctcatga ccagcaaccc
    21481 cattgtccac cgtcacaatc aaggaaatcc cttcttgctc gataaagtat ttataaacac
    21541 tagcattagg gccatagcca tcggtaaaac gatttggcag gtaaactcgg cactcagcac
    21601 caagttgttc caaactttcc ttcacaatag aagccgaagt catgccatcc gcatcgtagt
    21661 ctccataaac gagaatattt tccctttctt caatagcctg acgaatccgc tccactgcct
    21721 tgtccatatc atggagcaga taagcatcat gtaagtcctc caaggaaggt tctaaaaact
    21781 tcttcagact ttcttggtcc tgaatccctc tctcaaacaa taaccgagcc acctcaggac
    21841 ccagtccagc cttcttggct atctttgtaa aatccgcatc ttctacctgc agggcaaact
    21901 gccattcata agtaggtgtt atcaaaaaga catccactct ttctaagaat tctattgctt
    21961 cattataaca tgatttagac gttttctcta tccagattgc ttttttataa
//
LOCUS       DAVKAO010000029        21125 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKAO010000029 DAVKAO010000000
VERSION     DAVKAO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21125
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.029"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            111..3212
                     /locus_tag="VWX16_001142"
     CDS             111..3212
                     /locus_tag="VWX16_001142"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9158507.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDISLKLPRFNPARPAVEELRERAELGFVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKSEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3295..4302
                     /gene="pfkA"
                     /locus_tag="VWX16_001143"
     CDS             3295..4302
                     /gene="pfkA"
                     /locus_tag="VWX16_001143"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9158508.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTVMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4361..5866
                     /gene="pyk"
                     /locus_tag="VWX16_001144"
     CDS             4361..5866
                     /gene="pyk"
                     /locus_tag="VWX16_001144"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9158509.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6130..6378)
                     /locus_tag="VWX16_001145"
     CDS             complement(6130..6378)
                     /locus_tag="VWX16_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9158510.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRSNLDK"
     gene            complement(6545..6770)
                     /locus_tag="VWX16_001146"
                     /pseudo
     CDS             complement(6545..6770)
                     /locus_tag="VWX16_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7618..8490
                     /locus_tag="VWX16_001147"
     CDS             7618..8490
                     /locus_tag="VWX16_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9158511.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8636..8980)
                     /locus_tag="VWX16_001148"
                     /pseudo
     CDS             complement(8636..8980)
                     /locus_tag="VWX16_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9123..9435
                     /locus_tag="VWX16_001149"
                     /pseudo
     CDS             9123..9435
                     /locus_tag="VWX16_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9439..10155
                     /locus_tag="VWX16_001150"
     CDS             9439..10155
                     /locus_tag="VWX16_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9158512.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10285..11100)
                     /locus_tag="VWX16_001151"
     CDS             complement(10285..11100)
                     /locus_tag="VWX16_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9158513.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11097..12002)
                     /locus_tag="VWX16_001152"
     CDS             complement(11097..12002)
                     /locus_tag="VWX16_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9158514.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIISGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMFFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            complement(12248..12433)
                     /locus_tag="VWX16_001153"
     CDS             complement(12248..12433)
                     /locus_tag="VWX16_001153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158515.1"
                     /translation="MDKTDEVSSKHCFEVVDKTDEVSSKHCFEVVDRTDEVSSKHCFE
                     VVDRTDEVSNRTTVRRS"
     gene            complement(12422..12505)
                     /locus_tag="VWX16_001154"
                     /pseudo
     CDS             complement(12422..12505)
                     /locus_tag="VWX16_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            12549..14678
                     /locus_tag="VWX16_001155"
     CDS             12549..14678
                     /locus_tag="VWX16_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9158516.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14665..15114
                     /locus_tag="VWX16_001156"
     CDS             14665..15114
                     /locus_tag="VWX16_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9158517.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTRRYRCGLCRGKLLLINQPED"
     gene            15173..15445
                     /locus_tag="VWX16_001157"
     CDS             15173..15445
                     /locus_tag="VWX16_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9158518.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15493..15657)
                     /locus_tag="VWX16_001158"
     CDS             complement(15493..15657)
                     /locus_tag="VWX16_001158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158519.1"
                     /translation="MDEAGEILLILFKNQSANYINFTLPTKLQPAAGFLVCTLIFIEY
                     KSKKALNQAV"
     gene            complement(15804..15995)
                     /locus_tag="VWX16_001159"
     CDS             complement(15804..15995)
                     /locus_tag="VWX16_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158520.1"
                     /translation="MKHKEHILIGFLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16425..17183
                     /locus_tag="VWX16_001160"
     CDS             16425..17183
                     /locus_tag="VWX16_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158521.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17185..19173
                     /locus_tag="VWX16_001161"
     CDS             17185..19173
                     /locus_tag="VWX16_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158522.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TEKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVFRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASAQMSVLFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19215..19859)
                     /locus_tag="VWX16_001162"
     CDS             complement(19215..19859)
                     /locus_tag="VWX16_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9158523.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRGLAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(19831..20880)
                     /locus_tag="VWX16_001163"
     CDS             complement(19831..20880)
                     /locus_tag="VWX16_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9158524.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKKGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHRGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLVPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIHR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLNYKSPREDFLMANLNLKFSVRNKSRNRFK
                     FCRSFKYPARRCSWC"
BASE COUNT         6426 a         3866 c         4449 g         6384 t
ORIGIN      
        1 tacaaatgaa tagaaaataa attagtaagc tgatgaaatt tttctcaaga gaagccattt
       61 ataggtgaaa atggtataat atagtgagaa ggatagagga gaagtgtaaa ttgatcgcac
      121 aactagatac aaaaacagtc tatagtttta tggaaagcgt catttcgatc gaaaagtatg
      181 tgagagcagc taaagaatac ggctacactc atttggctat gatggatatt gacaatcttt
      241 atggcgcttt cgactttcta gagattacaa aaaaatacgg cattcatcct ttgctagggc
      301 ttgaaatgac agtgtttgta gatgatcagg aagtgaattt gcgcttttta gctctatcta
      361 gtgtgggcta tcagcagttg atgaagcttt cgacagccaa gatgcagggg gagaaaactt
      421 ggtcagtcct gtcccagtac ctggaggata tcgcggtcat tgtgccttat tttgatagag
      481 ttgagtcgtt agaactaggc tgtgattact atataggggt ttatccagaa acactagcaa
      541 gcgaatttca tcatcctatc ttacctcttt atcgggtcaa cgcttttgaa agcagggata
      601 gagaagttct tcaagtttta acagcgatta aagaaaatct accgctcaga gaagttccct
      661 tgcgttcgag acaagatgtc tttatatcag caagttcttt agagaaacta ttccaagagc
      721 gttttccgca agctttggac aatttagaaa agcttatttc aggcatttct tacgacttgg
      781 atattagtct gaaactgcct cgttttaatc cagctagacc agcagtagag gagttgagag
      841 agcgtgctga actggggttt gttcagaagg ggttgactag taaagaatat caagatagac
      901 tagaccaaga attgtctgtt attcatgata tgggctttga tgattatttc ttggttgttt
      961 gggatttgtt gcgttttgga cgatcgaatg gctattatat gggaatggga aggggttctg
     1021 cagtaggcag tttggtttct tatgccttag acatcacggg gattgaccca gtagagaaaa
     1081 atctgatttt tgaacgcttt cttaatcgtg aacgctatac catgcctgat attgatattg
     1141 atatcccaga tatttatcgt ccagatttta tcagatatgt tggtaataaa tatggtagta
     1201 aacatgcggc acaaatcgtt actttttcaa cctttggagc caagcaagct cttcgagatg
     1261 tcttgaaacg ctttggtgtg ccagagtatg aattatctgc aattactaag aaaatcagtt
     1321 ttcgtgacaa tcttaagtcg gcctatgagg gcaatctcca gtttcgtcag caaatcaata
     1381 gtaagttaga ataccaaaaa gcttttgaga ttgcttgcaa gatagagggc tatccaaggc
     1441 aaacctctgt ccatgcggct ggtgttgtaa ttagtgacca agatttaacc aactacattc
     1501 ctctaaagta tggtgatgaa attccactga ctcagtatga tgctcatgga gttgaggcta
     1561 gcggactttt gaagatggac tttctgggac tacgaaattt gacctttgtc cagaagatgc
     1621 aagagttgct tgctgaaaca gaaggtattc accttaaaat tgaagaaata gatttggaag
     1681 acaaagaaac gttagattta tttgcctctg gaaatacaaa aggtatcttt caatttgagc
     1741 aacctggtgc cattcgcttg ctcaaacgtg ttcaaccagt ctgttttgaa gatgtcgtag
     1801 caactacttc tctaaatcga ccaggtgcaa gtgactacat caataatttt gtggcaagga
     1861 agcatgggca ggaagaagtg actgttctag attcagctct ggaggatatt ttggctccaa
     1921 cttatggcat tatgctctat caggagcagg ttatgcaggt tgctcagcga tttgctggat
     1981 ttagtcttgg gaaggccgat attttgcgtc gagctatggg taaaaaggat gcctctgcta
     2041 tgcatgagat gagggcttcc tttattcaag gctccataga agcaggccat actgcggaaa
     2101 aatcagagca ggtttttgat gttatggaga agtttgcagg ttatggattt aatagatctc
     2161 atgcctacgc ctactcagcc ttggccttcc agttggctta tttcaaaacg cattatccag
     2221 ccatttttta tcaggtcatg ttaaattatt ccaacagtga ttacttaata gatgcacttg
     2281 aagcaggttt tgaagtagcc tctctatcca tcaatactat tccctatcac gataaaattg
     2341 ccaacaagtc tatctatata ggtttgaaat cgattaaggg gctcagcaag gacttggcgc
     2401 tttggattat tgaacataga ccttattcta acattgaaga ttttatagct aaattacctg
     2461 agaattatct gaaacttcct ctgctagaac ctttggtaaa agttggtctt ttcgattcat
     2521 ttgaaaaaaa tcgtcaaaaa gtatttaata acttagctaa tctatttgaa tttgtgaaag
     2581 agttgggaag tttgtttgga gatgctattt atagttggca ggaatcggaa gattggacgg
     2641 aacaagaaaa attttatatg gaacaagagc ttttagggat aggtgtcagc aaacatccac
     2701 tacaagctat tgcaagtaag gctatttacc cgattacccc aatcggaaat ttgtcagaaa
     2761 atagctatgc tattattttg gttgaagttc agaaaataaa agtgattcgt accaaaaagg
     2821 gtgaaaatat ggccttctta caggcagatg atagtaagaa aaaattggat gtcactctct
     2881 tttcagactt atatcgtcag gttggacagg aaataaaaga gggagccttc tactatgtaa
     2941 aaggaaaaat acaatcacgt gatggccgtt tgcaaatgat tgcacaagaa ataagagaag
     3001 cagttgctga acgcttttgg atacaggtga aaaatcatga atcggatcaa gaaatttcac
     3061 gtattttaga acaatttaaa ggcccaatcc cagtcatcat ccggtatgaa gaggaacaga
     3121 aaaccatcgt ttctccccat cattttgtag ctaaatccaa tgaattagag gagaaattga
     3181 atgaaatcgt tatgaaaacg atttatcgct aaaaatacgg aaaatagaag aattttaaaa
     3241 tcaaatgtga tataatcaag aagaatgtta aaagaaaaag gagcataaac caatatgaaa
     3301 cgtattgctg ttttgactag tggcggagac gcacctggta tgaacgctgc tatccgtgca
     3361 gttgttcgtc aagcaatttc agaaggaatg gaagtgtttg gtatctatga cggatatgct
     3421 ggtatggttg ccggtgaaat tcatccccta gatgcagctt cagtaggaga catcatttct
     3481 cgtggtggta cttttcttca ctcagctcgt tacccagagt tcgctcaact tgaagggcaa
     3541 cttaaaggga ttgagcaatt gaaaaaacac ggaattgaag gtgtagttgt tatcggtggt
     3601 gacggatctt accacggcgc tatgcgtttg actgaacatg gcttcccagc tattggtctt
     3661 ccaggtacaa tcgataacga tatcgttggt actgacttta caatcggttt tgacacagcg
     3721 gttactactg tcatggacgc tatcgataag attcgtgata catcatcaag tcaccgtcgt
     3781 acttttgtaa tcgaagttat gggacgtaac gctggtgata tcgctctttg ggctggtatt
     3841 gcaactggtg ctgatgaaat catcatccct gaagcaggct tcaagatgga agatatcgta
     3901 gcaagcatca aagctggtta tgaatgtggt aaaaaacaca atatcatcgt cttagctgaa
     3961 ggtgtgatgt cagcggctga atttggtcaa aaacttaaag aagctggaga tacaagcgac
     4021 cttcgtgtaa cagaacttgg acatattcaa cgtggtggtt ctccaactgc gcgtgaccgt
     4081 gttttggcgt cacgtatggg tgcacatgct gttaaacttc ttaaagaagg tatcggtggt
     4141 gttgcggttg gtattcgtaa cgaaaaaatg gttgaaaatc caattcttgg tactgcagaa
     4201 gaaggggcat tgtttagcct tactgcagaa ggtaagattg tggttaacaa cccgcacaaa
     4261 gccgatattg agctatctag cttgaataag agcttgtcat aatttacaat ttagttaata
     4321 agaccaaaaa ggtcatatat ataaaggagt cacaaaaatc atgaacaaac gtgtaaaaat
     4381 cgttgcaact ttgggtcctg cggtagaaat ccgtggtggt aaaaaattcg gtgaggacgg
     4441 atactggggt gaaaaacttg atgttgaagc ttcagctaaa aacattgcta aattgattga
     4501 agctggtgct aacacattcc gattcaactt ctcacacggc gaccaccaag aacaaggtga
     4561 gcgtatggca actgttaaac ttgcggaaaa aattgcaggt aaaaaagttg gtttccttct
     4621 tgatacaaaa ggacctgaaa tccgtacaga attgttcgaa ggtgaagcta aagaatattc
     4681 atacaaaact ggtgaaaaaa ttcgtgttgc aactaaacaa ggaatcaaat caactcgtga
     4741 agtgattgcg ttgaacgttg ctggtgctct tgatatctat gatgatgttg aagttggtcg
     4801 tcaagttttg gttgacgatg gtaaacttgg tcttcgtgtg gttgctaaag atgatgcaac
     4861 tcgtgaattt gaagttgaag ttgaaaacga tggtatcatc gctaaacaaa aaggtgtgaa
     4921 catccctaac actaaaattc ctttcccagc tcttgctgaa cgcgataacg acgatatccg
     4981 tttcggtctt gaacaaggta tcaacttcat cgcaatttca ttcgtacgta ctgcaaaaga
     5041 tgtgaacgaa gttcgtgcaa tctgtgaaga aactggaaac ggacatgttc aattgttcgc
     5101 taaaatcgaa aaccaacaag gtatcgataa cttagacgaa atcatcgaag cagctgatgg
     5161 tattatgatt gctcgtggtg atatgggtat cgaagtaccg ttcgaaatgg ttccagttta
     5221 tcaaaaaatg attatcaaga aagtcaatgc tgcaggtaaa gttgttatca ctgcaacaaa
     5281 catgcttgaa acaatgactg aaaaaccacg tgcaactcgt tcagaagtat cagacgtatt
     5341 caacgctgtt atcgacggaa ctgacgctac aatgctgtca ggcgagtctg caaacggtaa
     5401 atacccactc gagtcagtaa ctacaatggc tacaatcgac aagaacgctc aagctcttct
     5461 taatgaatac ggacgtcttg attcagattc atttgagcgt aactctaaga cagaagtaat
     5521 ggcttctgct gttaaagatg ctactagctc aatggatatc aaattggttg taactcttac
     5581 taagacaggt catactgcac gtttgatttc taaataccgt ccaaatgctg acatcttagc
     5641 attgacattt gacgaattga cagaacgtgg cttgatgttg aactggggtg ttatcccaat
     5701 gttgacagat gctccatctt caactgacga tatgttcgaa atcgctgaac gtaaagcggt
     5761 agaagcaggt ctcgttgagt caggcgatga tatcgttatc gttgctggtg tgccagtagg
     5821 agaagctgtt cgcacaaaca caatgcgtat ccgcacagta cgttaagaaa aatataaaaa
     5881 cctatcatat ccagctttct agcctgtatg atgggctctt tttttgtcca gggggcaaaa
     5941 ctttcataat ggattgatac tcttcgaaaa tcaaattcaa accacgtcag cgtcgcctta
     6001 ccgtactcaa gtacagcttg cggctagctt cctagtttgc tctttgattt tcattgagta
     6061 tgaaataaga tatgtacaaa ttgattagaa agtcaaatga atttctacaa atgttttagc
     6121 aatcgcaatc tacttgtcta gattcgatct gatatatttt cgatttaatg atatggtatt
     6181 taaaacctcc aaagtagctt gctccattct tttacttacg tgagtgtaga tgttatttac
     6241 tgttttagcg tttttgtgtt ccactctaac cattatagca ttcttctcag ctagtgtact
     6301 aaggagtgtg tgcctgaaaa tatgggaact aaggggctgg tttatcggtt tctctagttt
     6361 agtatttgcc ttttgcaaag tgatcttaaa agcctttctc taaatttaca tatcactatt
     6421 gtttaacaaa atctaatcta ttttaggtca cttattcttt ttttgaaatg tagaatgaac
     6481 tttttcaaag tttttcgaat cttttaaaat ctgtttgttt tatatcgcca ttctcccccc
     6541 ttttttaatt ctccctatat agcctgacaa ctttcccgat ggtacgaata tggttgcttt
     6601 cgtctaggtg gatgtcgggg tattcgggat tgagtttttt tgaggtagcc ttggcggagt
     6661 ttcttgacat agttagcgcc gtctacttgg aagatgccga tggtattata gtcaatctgt
     6721 ggggtattct tgataaatag gtagtcgctg tttcttatct ttgcctccat ggacttgctg
     6781 acgacataag cgattgggtc gtagtcgtct gggataatgg aaactccata actaaatcgt
     6841 cgtcctgcat cgagcggcta cctgcagaga taaactacct aacacgagag taagtagtct
     6901 gtctgtagtc gtccagtctg atgattttta cgatacttcg tttttctgct tatacagttg
     6961 cctctaggca taggttagga ctttaccttg tctgggtggt gcccgttggt cgtagataga
     7021 ttggatatcg ctaggagaat ccttttgaac tggaggaaag agggcatcaa tcaagctact
     7081 gaatacttta actaagtcaa atatagtatt tttcttagta gacctaaccc ttttttcata
     7141 atttctaatg gtgtttttac ttatacctat cttagtaccc aattcttatt gagtccaacc
     7201 attactagtc tatattgttt tatagttgat tgagtttgga atagtacgct gtagctgcta
     7261 aaacatttct aggaattaat ttgcctttcc taatagagtt gttcatatct tatttcaatc
     7321 tattatattt ttcgcctcta acaatcgcaa tctcttcttt atctacgaac gaaatcgctt
     7381 tctatttttg taagtaaagc atcacacgaa atccacgaaa atgaaaacct ttgttgtgtt
     7441 ttcgtaaaaa atttgttgac agagcacgaa acgcctacta taattaaatc aagtttaagg
     7501 aaataacaaa aaaacaaatc ggagggaaag acgatgaaca cattaaatga gaaagtaatc
     7561 aatatctgta aagcagtagt taaagaaact ttaatccaag acatttagga gaaagaaatg
     7621 aagaaatcaa gaaaattagc cacattagga atctgttcag ccttgttttt aggcttggct
     7681 gcttgtcaac aacaacatgc tacttctgag ggtacgaatc aaaggcaaag cagttcagcg
     7741 aaagttccat ggaaagcttc atacaccaac ctaaacaacc aggtaagtac agaagaggtc
     7801 aaatctctct tatcagctca cttggatcca aatagtgttg atgcattttt taatctcgtt
     7861 aatgactata ataccattgt cggctcaact ggcttatcag gagatttcac ttcctttact
     7921 cacaccgaat acgatgttga gaaaatcagt catctctgga atcaaaagaa gggcgatttc
     7981 gttgggacca actgccgtat caatagttat tgtcttttga aaaattcagt caccattcca
     8041 aagcttgaaa agaatgacca gttgcttttc ctagataatg atgcgattga taaaggaaag
     8101 gtctttgatt cacaagataa ggaagagttt gatattctat tttcgagagt tccaactgag
     8161 gcaactacag atgtcaaggt tcacgctgaa aagatggaaa cattcttctc acaatttcaa
     8221 ttcaatgaaa aagctcgaat gctgtctgta gtcttgcacg acaatctgga tggcgagtat
     8281 ctgtttgtag gccacgttgg ggtcttagta cctgctgatg acggtttctt atttgtagag
     8341 aaattgactt tcgaagagcc ctaccaagcg attaaatttg ctagtaagga agattgctac
     8401 aagtatttgg gcaccaagta tgcggattat acaggcgagg gactggctaa gccttttatc
     8461 atggataatg ataagtgggt taaactttaa tagtgttatg atatgacagt aatcttagga
     8521 tttttatttc tatttttgat aagttggtgg gttatttcaa tcgttaatag ccagaaacca
     8581 cgcaaaaatg aaacatttat tggctatgtc caaagatacg atattgatgg gaatggctat
     8641 caatcattac cgtgtcctca gaactgagag gagttcttga aatcgtaaaa ccactttgaa
     8701 caagagttac ttcaacccat tggctccgac ggattaagtt gctttcgtga ataccaaaat
     8761 cagccgcaat ttgttcataa gtgcggtatt ctcgcacata ttgaagagtg gccataagaa
     8821 ggtcttctag gcttaattta ggttttcgtc caccttttgc gtgtttaagt tgataagctg
     8881 tttttaatac agctaatatc tcttcaaaag tcgtgcgctg aacaccaaca agacgtttaa
     8941 atcgtgcatc agttagttgt ttacttgctt cataattcat agaactacta taccatattt
     9001 tgtttcgcag gaagtctaat gctaaaatct tatcagtgag aagatttctt aaagtttatt
     9061 ctttgtagtt tcatctgtat tagtacataa tttaacttag agaaaatagg aggactaatc
     9121 gaatggaatt aaaagatttt acagaaaaag aacaggaaat gattaagaaa aggcttacaa
     9181 tgtctaatat tagtgacaaa gaaactactg agaagattct ggcgctagtc ccacaagact
     9241 tgattaagcg aatcccgttt tttgtcagaa aacatgctac aacacgtacg attaaacgca
     9301 tttcaattga ataccctgaa ctctacgctg tagctcaaac aagtggtgag attccagaaa
     9361 aaaaaacgcg aagaattgcg tcagattatc acaactatct ttgaacaaaa gatgaataag
     9421 catagtatta agtagagaat gaaaaaatat tttattggcg gtttgggaag caatgcctat
     9481 catagcaagg attttcttca agaactagat tcgcaggtct attttctaaa tccatatgaa
     9541 aagcatcttc gagatgaaac agaattgaaa tcatggttta aaaatgagat tgtagaggaa
     9601 gaatctatct gtctgatagg tcattctatt ggaggcgatt tagctcgtta tttcgcatcg
     9661 gaatttgaag aagtaaagaa actgattctt ttggatggtg gctatctaga tttagataag
     9721 attttacctt tggatacaga gttagaggaa actaaaaatt atatcaaatc tcaaattatt
     9781 tcggacttag atgttcttac ttctaaagaa aaatctgaag caaagcattg gtcagaaaat
     9841 atggagaaag ctgtaagaca gtcctatcac tggaatgttg agtataatag atatgagttg
     9901 gctataaatt atgaaaatat agaagcgata ctccgcctac ggagaaaaat acaagctttt
     9961 aagagagaag tgggagatac cttgtttatt agtcctcgct atcctaatga agctacatgg
    10021 agagaggaag ccctaaaaga attgccagac tattttgata ctattttttt agaaaacttt
    10081 ggccatgagc tttatactca agcacctaaa gaaatcgcta gtctaatgaa tgagtggctc
    10141 gcttattttc tatgagctac ttgtattgct ggaaatggta taagatgaaa atctacaagt
    10201 cactaatttt catataacca aaaaacgaac aggctctttt tctgttattc agaagagaac
    10261 ttgttcgttt ttatcttgat tttcttaaaa agctctatat acatagggat tttctggttt
    10321 atcgactttg gtaaagctgt cgatttccaa ggttggaagg ttttgtttga gttctttatg
    10381 gtagtgattg accagttttc ctttggctga aatccaagtg ttgttttcat actgccgggt
    10441 attgcccttg gtcagcaatc catagacacc agaatctgcg atgcagtgaa taatgccgaa
    10501 gcggaacaga aattggctat tggcatgact ggggtcatta tagacaaagc ctgtgaactg
    10561 gattgtctta ccctcaaatt catctggata gtcgtagata gcctccatga cctccatata
    10621 gttttcatta gtgatctgaa tactatcttg ggataagtat ttatccgccg ccgttcgcat
    10681 ttccttttca taggctgatt ttgaaaaata agaactggta tctggtttca aatattggct
    10741 tgtcgtccct tcgcttgtct gaatggctag atccgttcct tccgataggg ggaaatgata
    10801 acctttagca gaaacggtct gagaatccaa gctaacagtt gggaaagtta agccgatgac
    10861 aagtggaata gccagaagag aaatactcgt catcttggct aatcggctgt tcagatgact
    10921 gtgggttttg acttgcttca tccagatata caattgaacg atagccaaga taaaagaaag
    10981 caccatggag atataggcca gataggaata gtgcatgttg atgtactggt ttagtttgcc
    11041 cgacagatgg aggtaaatag tcagttcaaa atagccagct aaaactaaaa atcgaatcat
    11101 aggatgactc caatcaagag agaatagact aagacgacaa gagttacaat tgttatgaag
    11161 tgactgataa atcgtgcttt caagtaattt ttcatcatga gaatattttt gatatccagc
    11221 attggaccaa tgacgagaaa ggccagaact ggtgccaaac cgaaactcga gagaagagaa
    11281 gcacctataa aggcatccgc ctcactacag agcgaaagaa gaaaggctaa aatcatcaag
    11341 agcaggatgg caaaaagagg ggtcgcactg atagaggtca gaatccgagt cggaacgtag
    11401 acctgtatta tagaagcaaa gaggcagcca aataccaaat aacgccccgt atcaaaaaat
    11461 tcatcaatgg cctgcacaaa gacttgaaaa actttttttg cagaactcaa gtaagaaaaa
    11521 tcatgctcat gacaagccag acgattttct ttctgaatcg gttcttgcca gaaaaatcct
    11581 agaaaaattc ctagtattac agccacaaga atggaaccca gagctcgtaa tagggcgaca
    11641 tggaaggagt tgccaaaggc agaataggtc gcaaaaagaa caatgggatt gataacaggt
    11701 gctgtcacaa gaaaaggaac ggccgtgtaa cttggaacct ttttttccag aaaacgattg
    11761 atgatgggga cgattccaca ttcacaagaa gggaaaaaca taccgacgaa ggtcccaaaa
    11821 aagattctcc cccaacgatt tcgagggaga aaatgataaa ccttgtcagg tgtgatataa
    11881 acttcaatca gccctgagat aatgcttcct atcagaacga agggaagggc ttcaatgata
    11941 atggagagaa aaatggctcc agtttgaagt acactagatg ggagttgttg aaaagcagtc
    12001 atctagtttt tcttttctgc tttgactttt ttctctttgt catcagggaa agtaacttgt
    12061 ttcaaatcag ggagtttagc aaactcttca aacatcttgt ccaagtcgtc tcgttttgta
    12121 aattttacag ccataggtgt acctcgattc taaattctac ctctattata ctattatttt
    12181 gcggaaaagg gagtttttta atttgttttc gatagttgat actcttcgaa aatctcttca
    12241 aaccacgtca gcttcgcctt accgtagtac ggttactgac ttcgtcagtt ctatccacaa
    12301 cctcaaaaca gtgttttgag ctgacttcgt cagttctatc cacaacctca aaacagtgtt
    12361 ttgagctgac ttcgtcagtc ttatccacaa cctcaaaaca gtgttttgag ctgacttcgt
    12421 cagtcttatc cacaacctca aaacagtatt ttgagcagcc tacggctagc ttcctagttt
    12481 gctttttgat ttttattgag tatgaaaagg attggagaaa gccttccagc ttgtggtata
    12541 atagatttat ggataaaaaa tatgaaaaaa tctctcagga tttgggagtg acgttaaagc
    12601 aaattgatac cgttctaagt ttgacagctg aaggggcgac tattcccttt atcgcgcgtt
    12661 atcgcaagga catgactggt agtctggatg aggtggcgat taaggctatt attgatttgg
    12721 ataaaagtct gacaaatctc aatgaccgta aggaagctgt cttagctaag attcaagaac
    12781 aaggtaagtt gaccaaggaa ttggaagaag ctatcttagt tgccgaaaaa ttagcagacg
    12841 ttgaagaact ctatcttcct tataaggaaa agcgtcgtac caaggcaacc attgcccgtg
    12901 aagctggact ctttcctctt gctcgcttga ttttgcagaa tatagttgac ttagagaaag
    12961 aagctgaaaa gttcgtctgt gaaggatttg cgactggcaa ggaagccttg accggtgcag
    13021 ttgatatttt ggtcgaagcc ttatcggaag atgtgacctt gcgttctatg acttatcagg
    13081 aagtgctgag acactctaaa ctcacttctc aagccaagga tgaaagtctt gatgaaaagc
    13141 aggtttttca gatttattat gatttttcag agacagttgg aactatgcaa ggctatcgta
    13201 ccttggctct caatcgtggg gagaaacttg gtgtcttgaa gatcggtttt gaacatgcga
    13261 cggaccgtat tcttgccttc tttgctactc gtttcaaggt gaaaaatgct tatattgatg
    13321 aagttgttca gcaatccgtt aagaaaaagg tcttgcctgc tattgagcgt cgtattcgga
    13381 cagaattaac tgagaaagct gaagagggag ctatccaact tttttctgac aatctgcgca
    13441 atctcctctt ggttgctcca ctgaaagggc gcgtggttct tgggtttgac ccagcctttc
    13501 gtacaggtgc caagctagct gtcgtggatg caacaggaaa aatgctgaca actcaggtta
    13561 tttatcctgt taaaccagca tcagctcgtc aaatcgaaga agccaagaaa gatttagcag
    13621 atttaattgg tcaatacggt gtagagatta ttgccattgg aaatggaacg gccagtcgtg
    13681 aaagtgaagc ttttgtagcg gaagttctga aagatttccc tgaagtcagc tatgttatcg
    13741 ttaatgaaag tggtgcttct gtctattctg ccagcgaact tgctcgtcag gagtttccag
    13801 acttgaccgt tgaaaaacgc tctgccattt ctatcgcccg tcgtttgcaa gatcctcttg
    13861 cggaattggt caaaatcgat cctaagtcaa ttggtgtcgg tcaataccaa cacgatgtta
    13921 gtcagaagaa actatctgag agtctggact ttgttgtcga tacagtggtt aaccaagttg
    13981 gtgtcaatgt caatacagct agcccagctc ttctttcaca cgtagctgga ctcaataaaa
    14041 ccatttctga aaatatcgtc aaataccgtg aggaagaggg aaaaatcact tcacgctccc
    14101 aaatcaagaa agttcctcgt ttgggggcca aggcttttga acaggctgct ggtttcctcc
    14161 gtatccctga aagtagcaat atccttgata atacaggagt tcacccagaa aattacgctg
    14221 ccgttaagga actcttcaaa tgcttggaca ttaaggatct gaatgaagag gcccaaagca
    14281 aactcaagtc gctttcagtc aaggaaatgg cgcaagagtt agaccttggt ccagaaactc
    14341 ttaaagatat cattgcagat cttctcaaac caggtcgaga tttccgtgat tcctttgatg
    14401 ctccagttct ccgtcaagat gtcctagata tcaaggactt agtggttgga caaaagctag
    14461 aaggagtggt gcgtaacgtt gttgatttcg gtgcttttgt tgatatcgga attcatgagg
    14521 acggtctcat ccatatttcc catatgagtc gcaagtttat caaacaccct agtcaagtgg
    14581 tggcagttgg agatttggtg agcgtttggg ttaagaaaat tgatactgaa cgtgaaaaag
    14641 ttaatctgtc tctcctagct ccaaatgaaa ctgactgatt acgtcaagca ggtttcacta
    14701 gaagacttcg gcagaccttt tatccatcat gtccagtgga ataggcgtct acgttcgaca
    14761 ggtgggcgat ttttccccaa agatgggcat ttggatttta atcccaaggt ttatcaggaa
    14821 ctggggttgg acgtttttag gaaaattgtc cgacatgaac tctgtcatta tcacctctat
    14881 tttcaaggga aaggctatca acataaggat cgggatttta aggaactgtt gaaagcagtg
    14941 gatggattac ggtttgtacc atccttgccc aatagtaact ccaagccaat caagctctat
    15001 cgttgccaat cctgccagca aacttatcag cgcaagcgaa gaattgatac cagacgctat
    15061 cgctgtggac tttgtcgagg taaattgcta ctaataaatc agcctgagga ctgatgaaag
    15121 ccgagcctac ccgtgatata ctatgtctaa cctagcagaa agaggaaata tcatgaacag
    15181 taaattttat aaaatgagac gaaatcgcat gatttcagga gttttggcag gtctgtcaga
    15241 caagtggaac tttgatgtta cccttgtacg ttttctgttt gccattttta cagtcgcaaa
    15301 ctttggactt ggggtgatta tctatatcat cctagcttct atcatgccaa ccaaggaaga
    15361 aatcgaagca gaaatgtacg gaacaggccc acgtaaacgc aaagaagctc aagccattga
    15421 cgataatgaa ggctggtttt ggtgaggttt tacaaaagat ttggaatacg caaaaagctt
    15481 taaatcaata gtctaaactg cttgatttaa agctttttta cttttatact caataaaaat
    15541 caaagtgcaa actaggaagc cagccgcagg ctgtaattta gtcggcaatg tgaagttgat
    15601 atagtttgca ctttgatttt tgaatagtat taataatatt tcgccagctt catccacaaa
    15661 ctatagtgga ttggaactag aataggacac ctatacttat aaaaaattgt tagaaatcga
    15721 tttgactgtc ctgatcgatc tgtcctgttc tgttcttatt tcattttact ataaaagcag
    15781 taatttaaac gcagtttttt aatctatttt tgctgctgag acaatacatt tttggaaaaa
    15841 tggtggatgc tgataacaac tgataaccag caaataacat aagcagtgcc ggtaaattct
    15901 gtaccgataa agtgtgtgtg ttcaactaag agctgaccga tgaatggaga gagcagatag
    15961 agaaatccga tgagtatatg ttctttgtgt ttcattaaag cgcaccttct ttccttatag
    16021 gttgaacgac ttgcaagtgt taaattgaat ggtatgttat ctaggtaacc ttactaaaat
    16081 gttagttccc gcttcctcct tttcaaagta agccttttca cacttatatt ttacaacttt
    16141 aaaaatagga aatcaaatca ttttctaagt aattttgctt atatttcccc caatcttaca
    16201 aaaatgtaag attaaaatct cttttgtaag gttaggttat ggcaagcggt ctttttttga
    16261 tgtaaactag actcataaag aaaaaggagt aagaacatga aaaaattatt tcaagaaaaa
    16321 ttttcaaaaa aaccaagtca caaagaaatc gagcgtgttc agctgggatg cgctatgatg
    16381 caagcgacat ttcatttgat gggatattaa gaaaggagat tctcatgaca cttttagatg
    16441 taaaacacgt tcaaaaaatt tataaaacac gttttcaggg caaccaagta gaagccctca
    16501 aggatattca ctttaccgta gaaaagggtg actatgttgc catcatgggt gagtctggtt
    16561 ctggtaaatc aactcttctc aatattctag ctatgctgga taaaccaagt cgtggtcagg
    16621 tttacttgaa tggaactgac accgcaacca ttaaaaattc acaggcctct agcttccgtc
    16681 gtgaaaagtt gggctttgtc ttccaagact ttaacttgct agatactcta tctgtaaagg
    16741 acaatatctt gcttccgctt gtcttgtcaa gaagacctat tacagagatg atgaagaaat
    16801 tggtggtgac agctgagaat ctgggaataa accaattaca agagaagtac ccttacgaga
    16861 tttctggtgg tcagaaacag cgtgtagcag tagcccgcgc catcatcaca gaacctgaaa
    16921 ttctccttgc ggacgaaccg acaggagctc ttgactccaa gtcatctgca gccttacttg
    16981 atgtctttga tgaaatcaat gagcgcggcc aaaccattct catggtaacc cactcaacag
    17041 cagcagctag cagagctaaa cgtgtactct ttatcaaaga cggcattctt tacaatcaaa
    17101 tctaccgtgg agagaagaca gaacgtcaga tgttccaaga aatctctgat accttgactg
    17161 tcatggcaag cgaggtgaat tagtatgttt cgattaacca ataagttagc ggtatcgaac
    17221 ttgattaaaa accgcaaact ctactatcct tttgcgctgg ctgttctctt ggcagtcact
    17281 gtcacctatc tcttttactc tctaaccttc aatcctaaga ttgcggaaat ccgtggagga
    17341 acaaccattc aggctacact tggatttggt atgtttgtcg tcacccttgc gtcagccatt
    17401 atcgttctct atgccaatag ttttgtcatg aagaaccgtt ccaaggaact aggaatttat
    17461 ggcatgttgg gcttggagaa gcgtcatctt atcagtatga cctttaagga gttagtggta
    17521 tttgggattc taactgttgg agcgggtatc ggtattggag ccttgtttga caagttaatt
    17581 ttcgctttcc tgctcaaact aatgaaattg aaggttgagc tggttgctac cttccagacg
    17641 aaagttgtca ttacagtgct tgttgtcttc ggtttgattt tcctaggcct catgttcctg
    17701 aatgcccttc gaatcgcccg tatgaatgcc ctccagctct ctcgtgagaa agctagtgga
    17761 gagaaaaaag gtcgcttcct tcctctccaa accattcttg gttccataag tttaggaatt
    17821 ggctattatc ttgcccttac ggaaaaagat cctcttacag ccttaacaac cttcttcata
    17881 gctgttttac tggttatctt tgggacttat ctcttgttta atgcagggat taccgttttc
    17941 ctccaaatct taaagaaaaa taagaaatac tattaccaac caaataacct catatctgtt
    18001 tctaacttga ttttccgtat gaagaaaaat gcagttggac tagccaccat cgctatcttg
    18061 tcaacaatgg ttttggtaac catgtcagca gcgacaagca ttttcaattc ctcagaaagc
    18121 tttaaaaaag ttctaaatcc tcatgatttt ggggtttcag ggcaaaatgt tgaaaaagaa
    18181 gacttggaca aactcttgag ccagtttgca agtgacaaag gttataagat taaagaaaaa
    18241 gaagtatttc gttacactta ctttgctgtt gcgaatcaag aaggaaccaa gttaactatt
    18301 tttgaaaaag gacaaaaccg tgtccaacca aaaacagttt tcatggtatt tgaccaaaaa
    18361 gattatgaaa atatgactgg tcaaaaactg tctctatcag gaaatgaggt cggactcttt
    18421 gcaaagaatg agggagttaa agaacagaaa gctctaactc taaatgatca tcaattttct
    18481 gtaaaagaag aatttactaa agattttatt gtcaaccatg ttccaaatca gtttaatatt
    18541 ttgactgctg attacaatta ccttgttgtt cctgatttac aagccttttt ggatcaattc
    18601 ccagattcgg ctatctataa tcagttttac ggtggtatga atgtagatgc cagcgaagaa
    18661 gaacaactca aggtcgctga ggagtatgaa aactacctca atcaatttaa tgctcaatta
    18721 gacacagaag gtagctatgt ttatggtagc aatctagcag atgctagtgc tcaaatgagt
    18781 gtcctctttg gcggtgtctt ctttatcggt attttcctat ccattatctt tatggtcgga
    18841 actgttctgg tcatctacta caaacaaatt tctgaaggct acgaagaccg tgaacgcttt
    18901 attatcttgc agaaagtcgg tttggaccaa aagcaaatca agcaaaccat caacaaacag
    18961 gttttaactg ttttcttcct tcctttgctc tttgccttca tacatctcgc ctttgcctac
    19021 catatgctta gcctgatttt aaaagtgatt ggtgtactgg atacgactat gatgttgatt
    19081 gtgaccttgt ctatctgcgc tatcttcctc atcgcctatg tgctgatttt catgattact
    19141 tcaagaagtt atcgcaagat tgtgcaaatg taaaaaagat acctcgactt caaaatcgag
    19201 gtatttcttg tattctaaat gctgaaaagt tgttcgagca ggaaggtaac tcccatggtc
    19261 aagagaccaa tagcaaggtt ccgaatcata gctgttctag tcggatcctt tccaagtctg
    19321 gcactagtgt aaccagtgag aagaagggcc acaccgacaa taaggacagt agcagggatg
    19381 cgatattcac ttgggaatat ggtcactgac agcattggag gcaaacttct aaggaaaaag
    19441 gcaacgaagc tagaaatggc agcgtgccaa ggattggtaa attcttcata ctcaatccca
    19501 tatttttcct ctaccagagc cttgagtgga tttttaagaa agatcttatt ggtcaagagt
    19561 tgggcagaag ttttgcattc tccattttgg atataagcag catagaggga ttttttggct
    19621 agtcccctat cttggtctaa caagagtttt tctcgcgaaa cggcagcttc ctcggtatct
    19681 tttggagttg aaacggatac atattctcca ccagccattg aaaaggcacc agctaagata
    19741 gccgcaaatc ctgataaaaa gataatccag atattggtcg tggcactggc aaccccgata
    19801 accacaccag caatggaaat aattccatcg ttagcaccaa gaacacctgc gcgcaggata
    19861 tttaaacgac ctgcaaaatt tgaatcgatt tcgtgatttg tttctgacgc taaatttcaa
    19921 gttcaagtta gccatcaaga agtcttctct gggtgacttg tagttcaagc attttttagg
    19981 atagttgtta atccactttt cgatgaatgc gacttctttg ggagtcattt tcttggttcc
    20041 cttaggtaac catctatgaa tgagcctgtt gtgattctca ttagttcccc tttcccaaga
    20101 ggcatagggg tgcgcataat agatgtgctc ctcagaaaat acatcaaaca agcgattgaa
    20161 ttccgttcca ttatctgccg tgatggaaag aatcttgtgt tgttttaaga tgagttttag
    20221 agcctgattg accacctcag cacttttatt tggaaccaat cggatgatct gatgtctact
    20281 ctttcgatcc gtcaagacaa gcaagcagta gtttttcgct ctcgtaagta gaaccgtatc
    20341 aatctcataa tgcccattct ccaagcgaag attgatagct tcaggccgct gttcgatgga
    20401 ttgaccagca ggtttaaagt tggtgctagc ctgtttctta agcgcttttc cttttctagg
    20461 gtaaagcaga tcctgcttgc ttaaccccaa ttttccacga tgaatccaat agtaaatggt
    20521 tgaaattccc acgttaaccc ctttagccat aaccatcatt tcaggcgaaa atttttggtt
    20581 atgatagtgg agaatctttt cctttagttc cttggtcaag cttgatttct tgaccgagcg
    20641 cttgcgattg ttttcataag actgttgagc atagtcggca gaataaacct ctttgaagcg
    20701 cccttttcca agacattgtc ggactgtccc acgcttgatt tcagtgtgga tagtttgagg
    20761 aacttttcca agcagagagg caatttctct atttgatttc cctttttttt tccatctttc
    20821 gattaagcga cggctatcga ttgtcaaatg tttggctttt gtagtataat tgtcttgcat
    20881 ctctgcgcct ttcttgtgtt tgtggttgaa caacaagtat aacacagagg tgttttctta
    20941 tgcctacaag agctatcagc tagttgaacc atctaatttt taggagggat gggtggctaa
    21001 cttcattata gaactttcat ttacgaacat atagtaaaat gaaacaagaa cagaacaaat
    21061 cgatcaggac agtaaaatct atttctaaca atgttttaga agcagaggtg tactattcta
    21121 gtttc
//
LOCUS       DAVKAO010000030        19990 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKAO010000030 DAVKAO010000000
VERSION     DAVKAO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.030"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(102..1733)
                     /gene="rlmD"
                     /locus_tag="VWX16_001164"
     CDS             complement(102..1733)
                     /gene="rlmD"
                     /locus_tag="VWX16_001164"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9158525.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(1807..2781)
                     /locus_tag="VWX16_001165"
     CDS             complement(1807..2781)
                     /locus_tag="VWX16_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9158526.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(2855..3469)
                     /gene="pmp23"
                     /locus_tag="VWX16_001166"
     CDS             complement(2855..3469)
                     /gene="pmp23"
                     /locus_tag="VWX16_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9158527.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3469..4443)
                     /locus_tag="VWX16_001167"
     CDS             complement(3469..4443)
                     /locus_tag="VWX16_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9158528.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLAYKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4451..5707)
                     /locus_tag="VWX16_001168"
     CDS             complement(4451..5707)
                     /locus_tag="VWX16_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9158529.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(5769..6200)
                     /locus_tag="VWX16_001169"
     CDS             complement(5769..6200)
                     /locus_tag="VWX16_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9158530.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6202..6801)
                     /locus_tag="VWX16_001170"
     CDS             complement(6202..6801)
                     /locus_tag="VWX16_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9158531.1"
                     /translation="MDRIRQELENGGAVILPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(6788..7627)
                     /gene="prmC"
                     /locus_tag="VWX16_001171"
     CDS             complement(6788..7627)
                     /gene="prmC"
                     /locus_tag="VWX16_001171"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9158532.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALDLANENAKNQNLQ
                     IFFKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(7627..8706)
                     /gene="prfA"
                     /locus_tag="VWX16_001172"
     CDS             complement(7627..8706)
                     /gene="prfA"
                     /locus_tag="VWX16_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9158533.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(8703..9293)
                     /locus_tag="VWX16_001173"
     CDS             complement(8703..9293)
                     /locus_tag="VWX16_001173"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9158534.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPCCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9416..9598
                     /locus_tag="VWX16_001174"
     CDS             9416..9598
                     /locus_tag="VWX16_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9158535.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            9735..11108
                     /gene="mnmE"
                     /locus_tag="VWX16_001175"
     CDS             9735..11108
                     /gene="mnmE"
                     /locus_tag="VWX16_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9158536.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11155..12090)
                     /gene="dapA"
                     /locus_tag="VWX16_001176"
     CDS             complement(11155..12090)
                     /gene="dapA"
                     /locus_tag="VWX16_001176"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9158537.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDVKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12146..13222)
                     /locus_tag="VWX16_001177"
     CDS             complement(12146..13222)
                     /locus_tag="VWX16_001177"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9158538.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDSEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(13564..13636)
                     /locus_tag="VWX16_001178"
     tRNA            complement(13564..13636)
                     /locus_tag="VWX16_001178"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13601..13603),aa:Thr,seq:ggt)
     gene            complement(13736..14245)
                     /locus_tag="VWX16_001179"
     CDS             complement(13736..14245)
                     /locus_tag="VWX16_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9158539.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14243..14339)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14368..14799
                     /locus_tag="VWX16_001180"
     CDS             14368..14799
                     /locus_tag="VWX16_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9158540.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            14881..15258
                     /gene="mscL"
                     /locus_tag="VWX16_001181"
     CDS             14881..15258
                     /gene="mscL"
                     /locus_tag="VWX16_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9158541.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15300..16394)
                     /gene="hemH"
                     /locus_tag="VWX16_001182"
     CDS             complement(15300..16394)
                     /gene="hemH"
                     /locus_tag="VWX16_001182"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9158542.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGMRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENIKMPYLIKKMLEKAGKLPKE"
     gene            16548..17771
                     /gene="pepT"
                     /locus_tag="VWX16_001183"
     CDS             16548..17771
                     /gene="pepT"
                     /locus_tag="VWX16_001183"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9158543.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(17807..18139)
                     /locus_tag="VWX16_001184"
     CDS             complement(17807..18139)
                     /locus_tag="VWX16_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158544.1"
                     /translation="MDALLDQLSANSKDKTALLESTKAILVLNHQIQSKASASEETSP
                     ARNAEANGDNTSAENQPNATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTT
                     GNQEKPVE"
     gene            complement(18144..18437)
                     /locus_tag="VWX16_001185"
     CDS             complement(18144..18437)
                     /locus_tag="VWX16_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158545.1"
                     /translation="MSKANQLLERIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHFYLESNNGSDADF"
     gene            18402..18623
                     /locus_tag="VWX16_001186"
     CDS             18402..18623
                     /locus_tag="VWX16_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158546.1"
                     /translation="MDSFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(18760..18969)
                     /locus_tag="VWX16_001187"
                     /pseudo
     CDS             complement(18760..18969)
                     /locus_tag="VWX16_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
     gene            complement(19090..19232)
                     /locus_tag="VWX16_001188"
                     /pseudo
     CDS             complement(19090..19232)
                     /locus_tag="VWX16_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262445.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(19266..19406)
                     /locus_tag="VWX16_001189"
     CDS             complement(19266..19406)
                     /locus_tag="VWX16_001189"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9158547.1"
                     /translation="MLEITLKSPYQFAHILFQSTIVPHGGHYHFIPESDLSAGELAAA
                     KV"
     gene            complement(19450..19990)
                     /locus_tag="VWX16_001190"
                     /pseudo
     CDS             complement(19450..19990)
                     /locus_tag="VWX16_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5757 a         4207 c         3806 g         6220 t
ORIGIN      
        1 tattttctaa ttgatacctt gcaagaaagg aataatagct gttcctatac caagtaggaa
       61 tgtaataaat gcagaaagtc atatactgtc attcttttct attaaatcat tttgaagtgt
      121 ctcaaagcat ccatgatggc ttcttctttt tcaggtgtac actgtggaat aatttgttta
      181 tcctttttag acttgttgta atgttctcgt aattctattc cacatttctt ttttatctgt
      241 gcaatatata atgtcgaaac ttttaattca aatttattcc aaacatattc tttgatttga
      301 gcatatgttg ctttgctctc cgcacttgtc aaatccatct catccagctc aatttcaaca
      361 cttatgtgct tatcgacatc gagtttggac aaaagtgcta ccgtctcgac gtgatgcgtt
      421 tgaggaaata gatccaccgg ctggactttc ttcaattcat atcccaactc ttggtatagt
      481 ttaatatcac gcgccatggt tgcgacattg caggagatat aggcgatgcg atcggctcct
      541 gtttgggcgc ttgctttgat aaagctttct gtcaagccct tgcgtggagg atcaaccaag
      601 ataacggttg gttgaatccc atctttaagc caattcttca tagcattttc agctgtgtca
      661 cagacatagt gggcgtttga aatattgttc agctgagcat tttttttact attctcaacc
      721 gcttctggaa tcagttcaac accgtagact tctttgacat gcttggcgac tgataaacca
      781 atggttccaa taccagaata agcatcaata accacatcat ctttttttaa ctctgcaaag
      841 tcaatggctg tttgatagag tttctccgcc atttcagtat tgacttggta aaaggctggg
      901 ccagcgattt ggaagtcatt tcccaacatc tggtccgtaa tatagtcttg accataaaga
      961 gtgcgccact ccttaccaaa aatcgcattg gtattctggt cgttgatatt ttgcatgaca
     1021 gacacaatct ctgggaactg cttgataact tgttcaatca attggtcaac acgaaaaact
     1081 tttggacgag ttgtcaccaa aacgaccatg atttgtcctg aatagtgacc acgacgcacc
     1141 acaagattcc gaatcaatcc agactgttcc ttttcgtcat aaggttttaa atcaaaacga
     1201 cggagcaggt ctcgaagagc tactacgact tggtcaatga caggatcctg gataaagaaa
     1261 tcttcaaggg gcatgaggtt gtgcgaattc ttacggaaaa atcctgtttc caagacacca
     1321 ttcactcgac gaacgggcac ctgcgcctta ttgcgatact tgactggatg ttccatacca
     1381 agcgtttcag caacttctac atctgcaatt ccagcaatct tgtagagact gtccttgact
     1441 tgcttggttt taaacttgag ctgttctgga taagaaaggt gtcctaaatc cgcgattcct
     1501 gaacgcaggt aagccaaatc tagatcttga ttacggtgtg gtgactggac aaggtatttt
     1561 tcaacttttc caaagccaat ctttttattg accttgagga cacgcatgag aattttttca
     1621 ctcggtaaag cattctctac aaaaaagacc aaaccatcta ccttggcaac tcctgcccct
     1681 tcatgggtca aatcaacaat ttcaacttct acaatatcat ttttctttaa cattcttttc
     1741 tctttctatt ggccgacaaa aaagacggca acgttacggt taccatccat tataccatga
     1801 aatttcttaa gaaccaaaac tcttgaagaa gttgacaagg gcttgccaga gggagcttaa
     1861 gaagttacca ccgtcctcta gcgccttatc cgcatcaaag ttaaggttga tatttttaaa
     1921 actgtcgcca gcttgtgata cgatgctttg tttaaggtca tttaaggttt tagtgaaatc
     1981 tgcattgctg aggatatcac tctttgagag attcaaggca aaattgatga tgatattgat
     2041 ctggtttcct gttatgacct gatcaagttt gtaatttttt aaggtatctt caacaatctt
     2101 gcggatatct tcttctgtca gatttccctt gctttcttta gctttggcga gtcctgactt
     2161 gatatcagct agggcaacgt ttaatttatt agcatcatag cctgatttgt ccttgttttc
     2221 agcattgata tctgacaaag cctttagctc ttcttgagcc aaatctttat tggcttgcgg
     2281 caccttggct ccattagctt ctagcgaata gtagatccca gccaaagcac tctcacctgt
     2341 aactggaatg ggtgctgcta cagtgatttt ggcgtgttcc acacccaaag ttacggctgc
     2401 atttcggtac atatcctgag ttaccttagt gatgttttca ggtgtttcaa tcttgacctc
     2461 aagtggcgat ttgtcaccta gcttttgaat cttggctgat gaatacaact gtaagctaga
     2521 gtcattggcc acattcatga ttttagaata aacatcaggt gtcatggtct tgagttcttt
     2581 ggtatctgtt gaggcattgt agcccagttt tttaagagtt tgattttttt ggtcttcaga
     2641 tagggaagaa cctaggacat attcaggttg gacataggtt tcatcgataa ctttttgaac
     2701 atctgttgct gcatggacgc tattcatagc tgttactgcc cacaagatcg cagcgctagt
     2761 cagaaagagt ttctttctca tagggaattt cctcctttac ttctttagag taatatatct
     2821 atcttaaaga aaacttataa caaaaacacc tggtctagcc agatgttgaa aagagagtga
     2881 aacatttgat gatgtaaagg ttaagtcgta cctgtctaga ataataatag tttcctccat
     2941 ttacatagag ttcagcaccg tgaaaaatgg aaatggggtg aatataacta taagtctttc
     3001 cagtcctatt accaagcaag ggggcaacag tctcacgaga gtactgtttg gctagagcca
     3061 aggtattttc cttgccattt tgggcgataa aatcgatata ggcaggtcca aaattatagg
     3121 cttgaacagc tgtccagata tctacaccct tcttctgcgc cagatagaga ttgcctgtca
     3181 gagtttgaat gccttgccga atgctagagg cattatcatt gatggtgttg gtggaaccac
     3241 ttgcagactc actagactgc ataacatcgc cttcttttcc ttttgtttca gtataaatca
     3301 tagcaagcac aagctcttcg tttgctgggg tgtctttttc actcaatatt tctcgcacca
     3361 tgggttgata ggtcatgact tgtttgacat cttgatgaac gcggtaagct ttatagccag
     3421 caaaaaggaa gactgctagt acaagcactc ttcgaattcg tttaaacatt atttactttg
     3481 gatatcctcg atatttttga ttaagataga gtaggttcca ttttcgtttt ggataaactc
     3541 aacagactcg gcgtcttgat agacgttatt gggaacgatg agctcaattc catttgataa
     3601 ggagagtttt tggttttcaa atttctttaa ttggcgactg gcatcaattt catcaaattg
     3661 aacaggttct ggtacggctt ctttgacttg gtcaataaag ctcaaacgag ccgtcagatt
     3721 gttgtcaaaa aggtcattag ccaatttctc aggtgacaat tcattgcttt cttctaggtt
     3781 gttgaaaata gctgatttga ccttggattg aaattgaaaa tcatctgtgt taaaagattc
     3841 agcaattctc tgggctgttt tttccagttc cttgatagat tttttaggag aaatcttagg
     3901 agcgacagca agaagattat ctgaaaaata gttcaaaaaa gtcccgttgt acttgattcg
     3961 tttttcaatc aggtgatact tgcgactctg aagattgacc accaaggcct cgtcagcacc
     4021 cgttccaaat ccaggcaggt tattctgagt cagcttgatt ggattatcaa cttctcctcc
     4081 gaggtgggtc aaggtctccc gcagggcaat tcgcaagaaa gcgaaatgtt ctacaccttc
     4141 tttagaaaat tgaacaaaaa tcaagtcatt ggtcttgaga ttttcagaaa tgctaaactc
     4201 ctctttccag agattagcca gcgttactga tgtctccaac aaatcgtctg taatatgatt
     4261 gaagaaggga ttttcttctt cgaaaatccc agtcttggct tcatctgaat acacatgttc
     4321 aattttttta cgtaggtatt cttcgatttt tggagtaata ttgagaaact tatatgctaa
     4381 gaacagctcg gtatcatccg gactgaactg gtgaataatg gctttcttaa tataaatgtc
     4441 cataaaagtt ttagtcctcg tataatggga aggcatctgt caattctttg actgcacttc
     4501 tcacttcttc taatacagcc tcattttctg aattcttaag ggttttaatg atgagttcag
     4561 ccactttgcg actttcttct tcaccaaatc cacgtgcagt aatagctgct gctccgatac
     4621 gaatcccact tgtcttgaat ggcgacaagg tttcgtaagg gattgaattt ttatttaggg
     4681 taatattgac ttcatccagc aagttttgag caacttttcc gttttctaca accttagtca
     4741 catcaacaag gaagagatgg ttttcagttc cacctgaaat aatacggaaa tcagggtctt
     4801 gcaagaagac atctgccata gccttgctgt tcttaattac attggcagca tattccttga
     4861 aggctggatc caaaacttct ttgaaggaaa ctgccttagc cgccacaaca tgctctaaag
     4921 gaccgccctg aatacctggg aaaatagctg aattgatttt tttagcaagt tcttcgtcat
     4981 tggtcaaaat caaaccacca cgaggtccac gaagggtttt gtgggtcgtt gttgttgtga
     5041 tatgagcgta tggaactggg cttggatgaa ggccagccgc aaccaaacca gcgatatggg
     5101 ccatgtccac catgagcttc gcaccgacag catctgcgat ttcacggaat tttgaaaaat
     5161 cgataatttg agaatatgct gaagcaccag ctacaatcag ttttggtttt acttcttggg
     5221 cttgtttcaa gatagcatca aagtctaaga gttccgtttc aggatcaaca ctataagaaa
     5281 caaagttgta ggtttgacca gagaagctaa caggagcccc atgagtcaaa tgaccacctg
     5341 atgccaaatc cattcccata accgtatcac ctggctcaat caaggacatg taagccgcac
     5401 agttagcttg gcttcctgaa tgtggttgga cattggcaaa tttagcaccg aaaatttctt
     5461 ttgcgcgttc aatagcaaga gtctctacaa cgtctactac atcagttcca ccataataac
     5521 ggcgtcctgg gtaaccctcg gcatatttat ttgtcaagat agacccttga gctgccataa
     5581 cagccttgga aactacgttt tccgaagcaa ttaactcgat attattttgt tggcgttctt
     5641 cttctttggc aatagcattc cagagatcag catcatatgc tttaaaatca tctttgtcaa
     5701 aaatcatagg tcttctcctt tattgtgtga ctagtccatt agtttgattt tacaataaga
     5761 aaatcaaact aaaagatgcg aataaaccgt ttctgcattt tatcacaagt atagccaact
     5821 ttttcataaa atgcatgagc acccagacga tgattggcag aatttaagcg gataaaccca
     5881 taaccacatc ttttggcttc ttgttccaac ccttgtagta aacttttacc gataccttga
     5941 ccttgcgctt gaggtgaaac tgctaaagct aagatattaa atcctgcttt ggaatagagt
     6001 gattcgtaaa cttcagcgtg gacatatcca agtaagacat gattagctgc atcctcatag
     6061 ccaagtagga aatgatggga atcctgagac agtctagcta gttggctagc cgtttcctct
     6121 ggactaaaag tataacccaa agcctcttgg ttgatgtcac atatcgcttt cacatctgtt
     6181 tcttgcaaat ctcttagcat ttcaagcctc ctcaaaagaa atctctggca accgagcaag
     6241 aatatcttct cgtttaattg cgccttggcg taagattttc accttgtctc cagacaaatc
     6301 cacaatagtt gaatcctgtc cagttagaaa agcatcgtct tccagaccca gaacctcttg
     6361 gtcaaaatcc ttcagaattt gttcaaaggt tacaccactt gcctgacctg agatattggc
     6421 agacggccca atcaagggac ctgtctctcg aattaaatcc agtgtgatag ggtgactggg
     6481 catccgaaat ccaatagttg caaggtcaga atttacccaa tagggaactc ggtcattggc
     6541 ttcgagaata atggtcaagg gacctggcaa aaaggtctct acaagttttt gtagataagc
     6601 tggctgattc ttagaaaagt gcaagatatc ctctaaagag gcaacattga gattgagcgc
     6661 cttgtctcta ggacgacgtt tgagctggta aacatggtca actgcttttt cgtctaaggc
     6721 cttggcaaaa agaccataaa ccgtctctgt aggcagaatg acagctccac cattttccaa
     6781 ctcttgtcta atcctgtcca tcatcaacca cgaccatcct atcttgacca aattggtcct
     6841 tgagtgttcg aacttttttt tcaggaagat gttttctaaa aagttcggga acactttgac
     6901 cttgcttgta tccaatttca aggtaaatct taccaccatc tttgagatag tcttttgcat
     6961 cttccgcaat tctacggtaa atagctaggc catcctcgtc tgcaaagaga gctagatgag
     7021 gctccgaata caagacattc aagcctacct ctgactcatc ttcacgagag atatagggtg
     7081 gattggaaac aattatatca tatttttcag aaatttctgt aaaacagtca gattttttaa
     7141 aaaatatttg aagattttga tttttagcat tttcgttagc taaatctaaa gcctcttgag
     7201 aaacatctgc tgctgtcact gaccaatctg gtctattttt tgctagagcg agagcaatag
     7261 ctccactacc tgttccaata tccagaactg aaagattcgt ctcaagattt tcagttagaa
     7321 taagctccac taactcctct gtttctggac gaggaatcaa aacccgttca tccaccttta
     7381 aatgcattcc ataaaaatct gcctgtccaa tgatgtactg agctggcttg tgagctgcta
     7441 gttgctggta aatatcttct acaaattgtt tttcttcctc tgttgtcacc tcctgctgga
     7501 gggcaaaaat aaagtctgta aaagatagat ttttcagact acgatagaca aaagagaggc
     7561 tttccgcttc ctctccttgt cttatcaact cttcttcaaa atttgaaaat aattgagcta
     7621 atttcattat ttgtttaatt cttctagttt ttgtgtttgg tcataaagca ccaaggcatc
     7681 cacaacttcg tccaatttac cagacaaaat cgtatctagt ttttggaggg tcaagccgat
     7741 acggtggtct gtgacacggt tttgtgggaa gttataagtt cggatccgtt ctgaacggtc
     7801 accagtaccg attgtcgact tacgctcagc gtcttgttca tcctgagcaa tctgagcaaa
     7861 gtggtcagcg acgcgtgcac ggataatctt catagccttc tcgcggttct tctgctgggt
     7921 acgttcttcc tgcatctcaa ccttgatatt ggttggcaag tgaacgatac gaacggcagt
     7981 cgcaacctta ttgacgttct gtccaccagc accagaggcg tgatagatgt cgacacgaag
     8041 gtcttttgga tcaatgtcgt attcaacctc ttcaacttct ggcataacaa gaactgtcgc
     8101 tgtcgaagta tgaacacggc cttggctttc tgtcacagga acacgttgca cacggtgggc
     8161 acctgattca tacttaagct tagagtatac agactgacct gaaaccatag caaccacttc
     8221 tttaaaacca ccgacaccat tcatagaggc ttccatgact tcaaagcgcc aaccttgggc
     8281 ttccgcatac ttttggtaca tagttagcaa atctccagcg aaaagtgccg cttcgtctcc
     8341 accagctgct ccacggattt caaggatgat attcttgtca tcgtttggat cctttggaag
     8401 gagcaaaatt ttcagttttt cttcatattc ttctttttca gccttggcat ctttgagttc
     8461 ttgcttggcc aattcttcca agtccgcatc tccgcctgat tccttaatca tctcttcggc
     8521 atcgacgata ttttgaagga cttgtttata ctcacggtag gctattacgg tgtcacgatt
     8581 tgaagcttct tcttttgaaa gctccataaa acgcttggtg tctgaaacga catcagggtc
     8641 actcagcaat tctcctagtt cttcataacg gtcttctaca gcttgtagtt gatcatagat
     8701 gttcattttt cttcattctc cttattgatt tcaggggcaa aataatgttt acggcaaacc
     8761 gagatatagg tttcattacc accaatctgg atctgttctc catcgtaaac gggcagtcca
     8821 tcctgtgttc gcaacaccat ggtcgccttt ttcttgcaat actgacagat ggtcttgatt
     8881 tcgtcaatct tgtccgctaa aagcaagaga tatttggaac cttcgaacaa ttcattgcga
     8941 aagtcatttt tcaagccaaa agccatgacg ggtatgtcta actcgtccac aacacgagct
     9001 aggtcgtaaa catggtggcg tttaagaaac tgggcttcat cgaccaaaac acagcaaggt
     9061 ttttctggta ggtctcggat atagccaaag atatccgttg tttcctcaat cgcaagggca
     9121 gggcgtttca tgccaattcg actcgacaca tagccaacac cgtcacgcgt atccagagcc
     9181 gaggtcataa tgacaacacc ttttccttgc tcctcgtagt tataggccac tttgagaatc
     9241 tcaatcgttt taccagagtt catggtccca taacgatagt acaactgtgc catgtttctt
     9301 gcttcacgtc catttctaaa tttttgctac attctagtat atcataattt tcttaagctt
     9361 taaacggcaa aatgtggtaa aatggaagaa atcaaaacta gtggaggaag ctattatgcc
     9421 atttgtacgc atcgatttat ttgaaggacg cacgctcgag caaaagaaag ctcttgctaa
     9481 ggaagtaacg gaagcagttg tccgcaacac tggagcccct caatctgctg tccatgtcat
     9541 catcaacgac atgccagaag gaacttactt cccacaaggg gaaatgcgta ctaaataagc
     9601 tagcttaagc agaattgctt aggctttttc aatctccaag tagcattcat tgaagaaata
     9661 tcctaaattt gttacaattt gaaaagaaac ttggagaatt tccaagaaaa gagctattaa
     9721 ttaaaggaaa cattatgatt acacgtgaat ttgataccat cgctgctatc tctactccac
     9781 taggtgaagg agctattggt attgtccgcc tgagcggaac agacagtttt gctattgcgc
     9841 aaaagatttt taaaggaaaa gacttgaaca aggttgccag ccacactctc aactacggtc
     9901 acattattga tcctctgact ggtaaagtca tggacgaggt tatggttggg gctatgaagt
     9961 ctccaaagac cttcactcgt gaggatatta tcgagattaa cacccacggt gggattgcgg
    10021 tgaccaatga aattctccag ctagctattc gtgaaggggc tcggttggca gaacctggtg
    10081 aatttaccaa acgtgctttt ttaaacggtc gcgtagactt gacacaggca gaggctgtga
    10141 tggatatcat ccgtgccaag actgacaagg ccatgaacat tgcggtcaaa caattagacg
    10201 gctccctttc tgacctcatt aacaataccc gtcaagaaat cctcaataca cttgcccaag
    10261 ttgaggtcaa tatcgactat cctgaatatg atgatgttga ggaagctact actgccgttg
    10321 tccgtgagaa gactatggag tttgagcaat tgctaaccaa gctccttagg acagcacgtc
    10381 gtggtaaaat ccttcgtgaa ggaatttcaa cggctatcat tggacgtccc aacgttggga
    10441 aatcaagcct tctcaacaac ctcttgcgtg aggacaaggc tatcgtaacc gatatcgctg
    10501 ggacaacacg agatgtcatc gaagagtacg tcaacatcaa tggtgttcct ctaaaattga
    10561 ttgacacagc tggtattcgt gaaacggatg atatcgttga acaaatcggt gttgagcgtt
    10621 cgaaaaaagc cctcaaggaa gccgacttgg ttctactagt gctaaatgcc agtgaaccac
    10681 tgactgcgca agacagacaa cttcttgaaa ttagccaaga taccaatcgc attattctac
    10741 ttaataaaac cgacctgcca gaaacgattg aaacttcgaa actacctgaa gacgttatcc
    10801 gtatttcagt ccttaaaaac caaaacatcg acaagattga agagcgaatc aacaacctct
    10861 tctttgaaaa tgctggcttg gtcgagcaag atgctactta cttgtcaaac gcccgtcaca
    10921 tttccctgat tgaaaaagca gttgaaagcc tacaagccgt taatcaaggt cttgagctgg
    10981 ggatgccagt tgatttgctt caagttgact tgactcgtac ttgggaaatc ctcggagaaa
    11041 tcactgggga tgctgctcca gatgaactca tcacccaact ctttagccaa ttctgtttag
    11101 gaaaataaga aaaatccatg atccttcatt cggtcatgga ttttattgtc tttattagta
    11161 atctggtctt aagacccctg ttacagttgc cttagttgct tcgtagtcgc catctacgac
    11221 aaccttgata atgcgtttga catcttcttc tggtgctgga acaagaggta gacgagtggg
    11281 tccagcttca aatcccatat agttaagaat tgccttaact ggagcaggac ttggataaga
    11341 gaagagagca ttaaccttag gaatgaattt acgctgaatt gctgcggctt tcttcatatc
    11401 gctttctgca atggcagtaa acatctcgtg catttcatcc ccatttgtat gagaggcaac
    11461 agaaataacc ccatccgccc caaggttcat ggcatggaaa gcatctccat cctcacctgt
    11521 ataaatcaag aactcttcag gcttgtgctc aatcaagtaa gccatattag ccaagctagt
    11581 acattctttg acaccgataa tatttggatg gtcagccaag cgaagcatgg tttctggagt
    11641 caattcgaca actacacgcc ctggaatgtt atagataata attggtaggt cagaagcatc
    11701 tgcaatagcc ttaaagtgct gatacatccc ttcttgagaa ggtttgttgt agtaaggaac
    11761 aatagcaagc ccagctgcga aaccaccaaa ttccgctact tctttgacaa actcaataga
    11821 gtcacgcgta tcattagtac ctacacccgc aatcaaagga acgcgtccat tgacaacctt
    11881 ttgtacagcc gcaaacaact ccaactcctc atcgtgggtc aaagttggac tctcagcagt
    11941 cgttcctgcg agaagaattc catccgtatg atgggccaat aaatgctcaa tcaaggctgg
    12001 aatagcatca aagttaatgg aaccatcctc atggaagggg gtaataaagg ctgtaatgat
    12061 tttacattct tttaaatctt gataagacac gaaaacacct ctctatttca aagaaggagt
    12121 tcatctgaac tcctaaacga tatgactatt ttaattcaaa tttcaattcg gctgttggac
    12181 gaaccaatcc acgttcatga agagtttcag caatctgaac tgagttccaa gcagcacctt
    12241 tgagaaggtt atctgaaaca acccacatgt gaattccttt ttctgaatcc aagtctttac
    12301 ggatacgacc aacaaaggta tcacgcgaac caactgcatt gatagcttga ggatagattt
    12361 gatgagctac atcatcttca agaacagcac ctgggaaggc tgcgatagct gcttttactt
    12421 cttcgattgg agccacttct tttgtttcga tataaacaga ctcagagtga gctgacaaga
    12481 ctggaatacg cacacatgtt gcagatactg caatgctatc atcttccata attttcttag
    12541 tttccttggt catcttcatc tcttcgtacg tgtaatcatt atcagtgaaa acatcaattt
    12601 gtggaagagc gttaaaggcg ataggataat gtttcttgtc accacctgaa ggcaagattt
    12661 ccgcatgcaa atcacgtggt ttcacaccat cattcaagac ttcacgaagt tcacgttgtg
    12721 tctcaagaat tgctcccata ccagcacctg aaacggcttg ataagttgaa acaatgatac
    12781 ggtccaagcc ccatttttgg cgaaccggct caagagccac catcatttgg attgttgaac
    12841 aattagggca ggcaatgatt ccgttgtgag catcaagtgc atgagcattg acctctggaa
    12901 caaccaaagg aacatctgga ttttgacgga aataagatgt attatctact actaccacgc
    12961 cagcttttac tgcgtatggt gcatacttag ctgatgtaga actacctgct gaaaagagag
    13021 caatatcaac tccttcaaaa gctgtttcag tcgtttcttc aattgtaata tcttgatctt
    13081 taaatttcaa tgacttacct gctgaacgtg cagaagcaag gtaacgaatt ttatcgattg
    13141 gaagtgttga ttcttccaac atttttatca tctgagcacc gacagcacct gtcgcgccga
    13201 ctacagcaac tgtatatccc ataaataacc tctttcggaa ttttctaaaa atttctataa
    13261 tagaagtatt atactacttt ttccatgaat tgcaaagctt tttcatcatt ttttggaaaa
    13321 taaaaatccc agtcgctaga ctagggacta agaggcatct tcatgtgatg agcaggttca
    13381 cacaactcat caaggtccgc tcctgcgtta tgacctcctt atgctcaata gtagtccgaa
    13441 gactacctat cgactcatcg catattctat attactagag atacttgatt ttgtcaataa
    13501 gaaatttttt cgatcgagta taacaaaaaa ctctcatatt caagagtttc cctatacata
    13561 tcatgctaat tgccgggatt gaaccggcga cctcatcctt accatggatg cgctctgcca
    13621 actgagctaa atcagcttac ttaagaagta tactatattc agaaaaactt gtcaagtttc
    13681 cttagaaatt ttccatagga aaaagccagg taagaactac ctagcttatc ttcttctatt
    13741 tacttaaatc aattcgacga ccaattttac caataataat cgccccaata atcaatgaag
    13801 caaattcacc gattcctgtt gtaaaccaag taaggaagaa tggtaattct gctacaatat
    13861 ttaactctgc agcgatagta aacatggaaa ttgaaaagag gattgaaaag aagaaatgat
    13921 cttttcgaat caatccgtta aagaggtaat ccttgctata ttttgcaaaa agccaaacac
    13981 ctaaactgag gaatactaag gttgaaccac caccaacaaa gacatctagc agtccgaagc
    14041 taaagaaatt agcaatcata caaccgatgg taactccgat gatgtactta ggattgtaaa
    14101 aagccataaa gttcatcatt tctgaaatac gaaactgata agcaccatag ctgatggcat
    14161 ttaggggcgg tgtgacagtc aaaacgacgt agatagcagc gacgattgca atatctgcaa
    14221 catcacgaat agttaatttt ttcatctttt ctcctttggc ggttatccgc gtgtaatatg
    14281 cttggtgaaa gaagctaagc accaagggtt gattcattca accttactag tatagcacaa
    14341 tagcctgctt tatgctatac ttaaagcatg aaaaaaccta ttcaaaaatt ttttaacaat
    14401 gaaatcttag cctatctctt ttttggcctt gcgacaactc tagtttcgat tctctcacga
    14461 ctagtcattt atcaactaag tcatcaggaa ctccttgcta cggccttggc aaatattatc
    14521 ggtatcttat ttgcctttat cacaaatgat acgattgtat ttaagcaaaa aaagaaaaat
    14581 agacttatcc gtttagtgaa attttctctt gctcgccttt ctacttttct gttagacttg
    14641 tttttaactt ttctctttgt aacccaattt cctcacatca tagggcaatt cgtaactgaa
    14701 aacattgata agataaatgc tattgaaaca ataattgcac aaatattcat tattatcatc
    14761 aattatatct tgagcaaggt ctatattttt agaaaataat gctccattta tgagcctttt
    14821 aattgccttc tataatgatt taggatacaa tgaaacacga aacattgaaa ggaaaacagt
    14881 atgttaaaaa atctaaaatc gttcttgctt cgaggaaatg ttattgacct tgctgtcggt
    14941 gttgtcattg cctctgcttt tggtgctatc gttacttcac ttgtaaacga cattatcact
    15001 cctcttattt taaatcccgc tttgaaagct gctaaagtcg aacgtatcgc tcaactttct
    15061 tggcatggag tcggctatgg taacttttta agtgctatta tcaattttat ctttgtgggt
    15121 accgccctct tctttattat caagggcatt gaaaaagcac agaaactgac tggcataaag
    15181 aaagaaaaaa ctgccgaaaa aaaaccaacc gaattggaag tccttcaaga aataaaagct
    15241 ctccttgaga aaaaataatc gattaaaagg atctagcgca aagctaaatc cttttttctt
    15301 tactctttgg gtaacttgcc tgctttttca agcatttttt tgataagata aggcatctta
    15361 atattctccc gccctttttt ctcaatcagt tttttcacaa attctggcat ttgtaaatct
    15421 tcagattcag ctaaaatgtt tttcgtctcg tctgaaggaa ctaactcatc aaaggtctct
    15481 tcttctggga gaaattcctt aaactcttga ttttcattga ctctaactgt attgaccaaa
    15541 gcatcaatca aacgagaatc tgtatttggc attggtggac gatggtagtt taccccaaat
    15601 tcctgacata aatcataaca ttccacatca ttgtcaaaca agacctcaat atgctcactg
    15661 atgaaactaa taggtacaaa aatataatgg tctggatgtt ctgtctgttc tctgagatac
    15721 tccaaaacat ctggtttaat ccatggaata ccaatatcac tctcactctg ccaagtgttg
    15781 gtatattgtt ctgaactcaa gcccagtttt tcagcgacta gcttgctatt ttcgaaaatt
    15841 tggtctatat aaggatctcc aaagtccaag gcaaaaatag gcacactatg ggctgaaaag
    15901 atgactttaa agctgtcttg ctttacttct tcttttaaaa tcttagcaat ttcatctgcc
    15961 caatagttta agagcgcttc ttcctgatac cagtccttaa tgaccaaaaa ttgaatttgt
    16021 ttgctttcta aaaatttttc atagcccatg acagagtaga aagaataatg gggctccaaa
    16081 atcaagcaaa tacactgttc aattccgtct gcttccattt gaccgatcac atctggaata
    16141 aagggactgg aaaacttatt agcaaagtag acactgtatt cattccccag cctagcttct
    16201 accaaggcaa cctcttgacg ggtaattttt tgcagaggcg tgcctccaat tcgtacataa
    16261 ttatcataga gagtctgaat ctcgtggtct tggggtctca tcccacgacg aatatttgtg
    16321 aaaaaatcag ctacaccttc aaaggtaatc tcttctggtg aaccgaaggt catcattaaa
    16381 attgcttttt tcatgactgc ttccttgtga tgtttttatc aagtttattt tatcattaat
    16441 tcatcgataa gtaaacgcta gcatagggac attctcaatc ttctgtcttt tgtttttctc
    16501 ttctttctat gatacaatgg aaaaaataaa ttcaaaagga gttttttatg acttatccaa
    16561 atctcttgga ccgcttctta acctacgtta aggtcaacac gcgctctgat gaacactcta
    16621 ctactactcc aagtacacag agtcaggttg acttcgcaac aaatgtccta attcctgaaa
    16681 tgaaacgtgt tggactgcaa aatgtttact atctaccgaa tggttttgct attggaacct
    16741 tgccagccaa cgatccgtct ttaacacgta agattggttt tatatcgcac atggatactg
    16801 ctgattttaa tgctgaagga gtcaatccac aggtaattga aaactacgat ggtggtgtga
    16861 ttgaactagg gaattctggt ttcaaactcg atccagccga cttcaagagt cttgaaaaat
    16921 atccaggaca aacgctcatc acaacagatg gaacaacctt gctaggtgct gatgacaagt
    16981 caggaattgc tgaaattatg acagccattg aatatctaac tgctcatcct gaaattaagc
    17041 actgtgagat tcgtgttggt tttggtccag atgaagaaat cggtgttggt gccaataaat
    17101 ttgatgcaga agattttgat gtggattttg cctacactgt tgatggtggt ccactaggtg
    17161 aacttcagta cgagactttc tcagccgctg gtgctgaatt gcatttccaa ggccgtaatg
    17221 tccaccctgg tactgccaaa gggcagatgg tcaatgccct tcagctagca attgattttc
    17281 ataatcaact tccagaaaat gaccgacctg agttaactga aggttaccaa ggtttttacc
    17341 atctaatgga tgtgacaggt agtgttgagg aggcgcgtgc aagctacatc attcgtgatt
    17401 ttgaaaaaga tgcctttgaa gcgcgtaaag catccatgca atctatcgct gataagatga
    17461 atgaagaact tgggagcaac cgtgtcactc tcaacttgac agaccagtac tacaatatga
    17521 aagaagtcat tgaaaaagat atgactccaa ttaccattgc taaagccgtt atggaagatc
    17581 taggtatcac gcctattatc gaaccaatcc ggggtggaac agacggctct aagatttcct
    17641 ttatgggaat cccaactccg aatatctttg caggtggcga aaatatgcac ggacgttttg
    17701 aatacgttag ccttcagact atggaacgtg cagttgatac catcattggc atcgtagctt
    17761 ataaaggcta aaaagacgag gtagctcagc tacttcgcct ttctttttat tctactggtt
    17821 tttcttgatt tccagtagtt gtagaagatt ctgttgtttt attttctgaa gttgattcag
    17881 caggtttaga atctcttgta ttgcttggtt tgttttcgtc gctagcagtt tcaatgttag
    17941 attctgcagt tgcgtttggt tggttctcag cactggtgtt atcaccattt gcttcagcat
    18001 ttcttgctgg acttgtttct tcacttgcgc tagcttttga ctggatttga tgattcaaaa
    18061 ctagaatagc ttttgtcgat tcaagtaaag ctgttttgtc tttactatta gcagaaagtt
    18121 gatctaataa tgcatccacc ttatcaaaag tccgcatcag atccattatt actttctaaa
    18181 taaaagtgaa gcgacatgag aatatcgtag agtttttgat agagtacaag tgtctgagga
    18241 tcttgctcag cattttcctt ttcttgttga agggcgctag cgatacgagt caagacatct
    18301 tttacctgac tgtttacttc atccaagtct gcatcagcct tgtttgtggc agcttttaga
    18361 ttttctactt cttctgccaa agattgtctg attccttctt catggattcg ttccaagagt
    18421 tgatttgcct tgctcaaaag actttctact tcttccttgc tatctgtcgc agattattgg
    18481 ttgctatcta ccatgtactc ctaaaacagg agagttataa tccaagatta caaggcctta
    18541 cagaaataag aaatccagat aagacaatgt tcgtccaaga cgctattcgc ttcgcacagc
    18601 agcacggatt caatatgctt taattttaaa gtttaggtgt caagacctct ttttagtgtg
    18661 cccaaaattt agagaagtaa tcaatcaact aacttttatt tttttcaaac tttcagtaaa
    18721 ctgacctaaa gctaactcaa tctgtctttg ttcgataggc ttgtctttgt agatgcttct
    18781 gctatcagct agaagttgat ctacttttgc caagactgcc ttctcatcaa aagttccagg
    18841 ttgatagttg gattgcaggg atggaatctt gtttttcaaa gccgcttcat atcccttagt
    18901 ttgaaccttg atgtagtgat tgtggtcgcc acgaggaatc acaaaacctt ctgaatcttc
    18961 acttataatt cgattggcat caaaaccatg accatcttct tcctcatggt ggacatgtag
    19021 tgacggatta cttaatacag aactagaaga acttcctacc ttttccgtgt tagagtgtga
    19081 tgggggattg ttaagagatg acttaggaat atagtgatag tgaccccatg tcttactata
    19141 taagcatcac ctgtatctct gacaatatca ttagggttaa agacataacc atcatctgct
    19201 gcagaaacac cattattcgg tgtcaccgac aaagattgac tgagagctgt agtattctct
    19261 gataattata cttttgcagc tgctaattca cctgccgaca agtcactctc aggaatgaaa
    19321 tgatagtgac caccatgtgg tactatagta gattgaaata gaatatgagc aaattgataa
    19381 ggggatttta aagtaatttc taacaatgat ttagaaacta tgatgtgcta ttctaaattc
    19441 aactcactat atataaccat catcggtagt ataacgtccc tgtaattttg ctacagatac
    19501 ttctgcacta gctcctttat cgtctttacc atgttcttgt ttttggcgat tgatttcatc
    19561 ttttattcgt acattttctg catgagcttg atctttaagg taaacataat actttccatc
    19621 taccttaata atatatcctc ccttaaccta actgacgata tcttgatctt tcggctgata
    19681 gttgggggct ttcattaata gctcttcact aaagagcgca tccaaaggaa ctttaccatt
    19741 atagcagtga taatgatcgc catgagaagt tacataacct tgatctgtaa tcttaataac
    19801 aatttgtttt gcttgaattc cttctttttg actaacctag tctggagtca aattttcagt
    19861 cttcttagtg tctttattac tgtttacata tgaaacacga tttttatctg tattggcctg
    19921 ttagctatgt tggttcagag cataaacaca cagacttaag gaaaggataa caacagatcc
    19981 agctgctata
//
LOCUS       DAVKAO010000031        19210 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKAO010000031 DAVKAO010000000
VERSION     DAVKAO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.031"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            71..295
                     /locus_tag="VWX16_001191"
     CDS             71..295
                     /locus_tag="VWX16_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9158548.1"
                     /translation="MYQRIRDLREDHDLTQKFVANLLSFSHTNYAKIKRGEVVLTADV
                     LVQLFKLYDVSTDYLLGLTDCPDRIKRKIK"
     gene            430..591
                     /locus_tag="VWX16_001192"
     CDS             430..591
                     /locus_tag="VWX16_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158549.1"
                     /translation="MTTNKDNDTSERSDCYRKRTAGETPMNDLTSHTHGGNYRIARYQ
                     EEKFWNKQL"
     gene            700..2472
                     /locus_tag="VWX16_001193"
                     /pseudo
     CDS             700..2472
                     /locus_tag="VWX16_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2577..3164
                     /locus_tag="VWX16_001194"
     CDS             2577..3164
                     /locus_tag="VWX16_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEU9158550.1"
                     /translation="MGGNPPIKKYSIVDKIVLSTKIKRIIIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADLIIIGGGNTEKYIATYVNQEFKNYIDHM
                     LNKGAKVIGFSAGALLLGEKVYVSPNDNSDHQIKIKDGLGLFSQFLISVYYDSWNDKA
                     NKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            3271..3675
                     /locus_tag="VWX16_001195"
                     /pseudo
     CDS             3271..3675
                     /locus_tag="VWX16_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            3869..4108
                     /locus_tag="VWX16_001196"
                     /pseudo
     CDS             3869..4108
                     /locus_tag="VWX16_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            4252..5481
                     /locus_tag="VWX16_001197"
     CDS             4252..5481
                     /locus_tag="VWX16_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9158551.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            5501..8197
                     /gene="ppc"
                     /locus_tag="VWX16_001198"
     CDS             5501..8197
                     /gene="ppc"
                     /locus_tag="VWX16_001198"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9158552.1"
                     /translation="MSLQKLENYSNKSVVKEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWHNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    8280..8497
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8610..9614
                     /locus_tag="VWX16_001199"
     CDS             8610..9614
                     /locus_tag="VWX16_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9158553.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            9683..10549
                     /locus_tag="VWX16_001200"
     CDS             9683..10549
                     /locus_tag="VWX16_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158554.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            10546..11304
                     /locus_tag="VWX16_001201"
     CDS             10546..11304
                     /locus_tag="VWX16_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158555.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLASYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            11394..13154
                     /gene="dnaG"
                     /locus_tag="VWX16_001202"
     CDS             11394..13154
                     /gene="dnaG"
                     /locus_tag="VWX16_001202"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9158556.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYQAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFTFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            13157..14266
                     /gene="rpoD"
                     /locus_tag="VWX16_001203"
     CDS             13157..14266
                     /gene="rpoD"
                     /locus_tag="VWX16_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9158557.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            14280..14609
                     /locus_tag="VWX16_001204"
     CDS             14280..14609
                     /locus_tag="VWX16_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9158558.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            14724..15767
                     /gene="cpoA"
                     /locus_tag="VWX16_001205"
     CDS             14724..15767
                     /gene="cpoA"
                     /locus_tag="VWX16_001205"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9158559.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVVAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            15783..17108
                     /locus_tag="VWX16_001206"
     CDS             15783..17108
                     /locus_tag="VWX16_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9158560.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            17139..17315
                     /locus_tag="VWX16_001207"
     CDS             17139..17315
                     /locus_tag="VWX16_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9158561.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            17374..17508
                     /locus_tag="VWX16_001208"
     CDS             17374..17508
                     /locus_tag="VWX16_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9158562.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            17572..18882
                     /gene="obgE"
                     /locus_tag="VWX16_001209"
     CDS             17572..18882
                     /gene="obgE"
                     /locus_tag="VWX16_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9158563.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            18892..19050
                     /locus_tag="VWX16_001210"
     CDS             18892..19050
                     /locus_tag="VWX16_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158564.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         6141 a         3205 c         4233 g         5631 t
ORIGIN      
        1 actgtcatat tatgacaatt tcccagcttt tttctgaaag tgacatatac ttttcttagg
       61 agaaagctag atgtaccaac ggataagaga tttgagggaa gatcatgatc tcactcaaaa
      121 gtttgttgca aatttacttt ccttttctca tacaaactat gcaaaaatta agagaggaga
      181 ggttgtctta acggcagatg ttcttgtgca gctttttaaa ctctatgacg ttagtacgga
      241 ttacttatta ggactaacag attgtccaga taggataaag cgtaaaataa aatagtctct
      301 ccttgatttg ttcattgaca attgaataaa ccgaggtggg acttttttat ttgtcgagca
      361 atttagtgag atacgccgtg ataggagcga aaattattag ctataaaata gagtggttct
      421 ctatctgcca tgaccacaaa taaggacaat gatacttctg aacgttcaga ctgctatcgt
      481 aaaaggacag cgggtgagac gcccatgaat gatctaacca gtcataccca cggaggaaat
      541 tatagaatag cacgttatca ggaggaaaaa ttttggaaca aacagttgta aaatatagtc
      601 tgcagttatc aatgcttaat tttttatatt ctaatttatt tgaacgcggt aatatagaat
      661 caaaaggagg aatttcaatt tgaaaaaaat aatgttgaaa taataaaagc tgattcttta
      721 gttcgacgta gaggtgtcaa tgtagaaaga catttaaaaa gagttgctgc ttattgccga
      781 gttagctctg atagcgaaga tcagaagaac agctatgaat ctcaggtgag acattataaa
      841 gattatatct ctcagcggtc ggattgggaa ctagcaggta tctatgcaga tgaggggatt
      901 tcaggaacac aagtaggaaa gagacaagat tttcaacgat taataaatga ttgtgtaaat
      961 ggagaaatag attacattgt cacaaaagca attgctagat ttgcaaggaa tactctagat
     1021 actttgaagt atgtcagaat gttaaatgat atgcaaattg gagtatattt tgaggaagaa
     1081 aacatagata tcttaacaat ggatggagaa ttgcttctga ctattctaag ctccgttgca
     1141 caacaagaag tagaaaatac ttctgcccat gtgaaaaaag gattaaaaat gaagatggaa
     1201 cgtggtgaat tggttggctt tcaagggtgc ctagggtatg attatgatgt agaaaccaag
     1261 cagatttcaa ttaataaaaa agaatccaaa attgttcgtt acatttttga aaggtatcta
     1321 gaaggtattg gaggaaaagt aattgcaaga gaacttgatg aacttggtta taaatcgcca
     1381 agaggattag aacattggaa tgatacaaca gttctaggaa tcataaagaa tgaaaaatat
     1441 aaaggtgata ttttaatagg aaaaactttt acagttgacc caataagcaa gagaaggttg
     1501 agtaattttg gagaagaaga taagtactat atcaaagata atcatgaacc tataatatct
     1561 aaataagatt ttgaaaaagc tcaggagata agactgcgtc gagcaggaaa taagaagact
     1621 gctgcaaatg taaatggcaa gcgtgaacgc tattcaaaaa tgtatgcttt tagtagcatg
     1681 ttagaatgtg gtttttgtgg ttcaatatta tctagaagaa gttgtcactg ccgttcagat
     1741 tatcgcaagg ttgtgtggca ctgtgttaca tcgataaaga aaggaaagaa attttgtaag
     1801 catagtaaag gattagaaaa gctcgctatt gagggggctt tcatggaagc ttacagacaa
     1861 ctctatcatt caaatgaaaa cttaatgaca gacttacttg aaacgattga atctgaattg
     1921 aatgacaata gccttaacaa ggaactaaaa aggattacaa acaaacttcg aacattacta
     1981 aaaaaagagg aaaatcttgt aaatctaagg ctcgaaggga aaattagcga taccatatac
     2041 gatgaaaaat acaatgaaat ttcttcagaa aaagagtttc tagcagaaga gaaggtaaat
     2101 attgaaacaa ccttaaaatc agaaatcgat gtaaagaaaa gactaactga gtttaagcac
     2161 ttgctatctt cgcaaaaaat gcttacggag tttgatcgtg cagtttttga aagtattgtt
     2221 gagaaaatta ttgtaggtgg agttaatagc aacggagaaa ttgatcctgc aatgttaacc
     2281 ataatattca aaacaggtga aactcaaaac aaggatggta aacaatttaa aagtaaacgt
     2341 aaaaatgcta aactagaaac agataaattg tgtcctcaaa acagtgacga ggataaaaaa
     2401 ttgtattctc aaggaacaga caacacacgt ggagtgtgta gtgttgctgg ttcgatctta
     2461 gtcagtcaat aaaatgatat gttttaaaaa aattataatt tcgaaacgtg aataaacttg
     2521 tttaaacttt atgtatcttt agaaagtgtg ataacttgaa agaacaaatt tttttgatgg
     2581 gtgggaatcc cccaataaag aaatatagta ttgttgataa aattgtatta tcaaccaaga
     2641 ttaaaagaat tatcattttt acagtttttc gagaaaattg ggaaccgtac atgaaaaagt
     2701 acacggaagt ttttcaaagt caatttccta atctaaacat tgattacttg ctcctggaca
     2761 ctgagcagat tgatcttgat agctatctag atgctgatct aattatcatc ggtggtggaa
     2821 atacggaaaa atatatagct acttatgtca atcaggagtt caaaaattat attgatcata
     2881 tgcttaacaa aggggcgaaa gttatagggt tttctgcagg agccctatta ttaggagaaa
     2941 aagtctacgt ctcacctaac gataattcag atcatcagat aaagataaaa gatggattag
     3001 gactctttag tcagttttta attagtgtct attatgattc atggaatgat aaagcaaata
     3061 aggatagagc tgaagaactc gttaatgttc ccataattcc actaaatgat cattcatgtc
     3121 ttgtattaga taaacttgga aacattattg agaaaattga ctagtttcca ttatctaaat
     3181 cttgatttag gtaactaaag tgtagacgaa ctggataggt tactagtgaa aactgcaaaa
     3241 agtgttgtga aatgaatcgg agagtgcttg atggatttta gtagtaagat agccataaat
     3301 acaggctggt cagatgataa aaaatattgt gtgacagatc aaaatcagca aaaatatttt
     3361 ttgcgtgttt ctgataagga gaagttagat tctaaaaaat ttgaatttga tatgatgggg
     3421 aaagtagctt ctcttggagt tcctatgtgt aaaccaatta gcattgaact ctgcgatgac
     3481 gaagtacatt ctttacacga atggatagac ggaagagatg caatagatag cattttaact
     3541 tattcagaaa accaacaata cacatatggt gtagaagcag gaaagatact tagaaagatt
     3601 catacaattc ctgctacaga agtttgtgaa gattgggaaa tcttttttaa tctaaaaatt
     3661 gatgataaaa tctccaacga aatgatatgg taagtccgaa acgctcagtg tgtaagcttg
     3721 ttgctaaaag cgtaaaaacc ttggaacgaa aggaataata gacttcctgc gaaacaaaaa
     3781 tacaatacaa taaaactatg gatgatgaag caagtaaaca attaagcgat agccgtttca
     3841 agagccttgt aggtgttcag cgcacgagat gttagctgtg ttaaaaacag cttatcaacg
     3901 caacgtaaac gcgcaaaagg tggacgaaaa accaaattaa gcctagacga tctcctcatg
     3961 gtaactattc aatacatgcg agaatagcgc acttatgaac aaattgcggc tgattttggc
     4021 attcacgaaa gcaacataat tagtcggagg tcaatggatt gaagcaactc ttattcaaaa
     4081 tggttttacg atttcaaatt ctgccttaat tctgtaaaaa cagtaaaatt cgaaggattg
     4141 taaggtgaga gtttttttct ttctgaaaaa atggtataat agcaatcaaa actagaaaat
     4201 aaaacggaat ttggaacaga tttgtctgta tcctagtaga gtggtgatac tatgaagatt
     4261 agtaagaggc acttattaaa ttattccatc ttgattccct acttgctttt atctattttg
     4321 ggcttgattg tggtctattc gaccaccagt gctattttaa ttgaagaagg taagagcgcc
     4381 ttgcagttgg ttcgaaacca aggaatcttt tggattgtta gtttgatact gattgcctta
     4441 atttataaat tgagactaga ttttttgaga aatgagcgac taatcatttt agttatatta
     4501 atagaaatgc ttttattgtt cttggctcgt tttattggta tttccgtaaa cggggcatac
     4561 ggttggattt cggttgcagg agtaactatt cagccagctg agtacttaaa aatcattatt
     4621 atttggtatt tagctcaccg attctccaaa cagcaagaag aaatagctac ttatgatttt
     4681 caagttttga ctcaaaatca atggcttccc cgtgctttta atgattggcg attcgttctc
     4741 ctagttctga ttggaagttt gggaattttc cctgatttag gaaatgcgac tattttagtc
     4801 ttggtttcct tgattatgta tacagttagt ggaatcgctt atcgctggtt ttcaaccatt
     4861 ctggcactcg tatctgccac ttctgtcttt gtcttgacca ctatcagcct aatcggtgtt
     4921 gagacctttt caaaaattcc agtatttggc tatgtagcca agcgctttag tgcctttttt
     4981 aatccttttg ccgatcgtgc tgatgcaggt caccagttag ctaattctta ttttgccatg
     5041 gtcaatggcg gttggtttgg tctaggtctt ggaaactcga ttgaaaaacg aggttatttg
     5101 ccagaagctc atacagactt tgtcttttct atcgtgattg aagaatttgg ctttgttggt
     5161 gccagtctta ttttagctct cttgtttttc atgattttgc ggattatctt ggtcggtatc
     5221 cgagcggaga atcctttcaa tgccatggtt gcactcggtg tcggagggat gatgttggtt
     5281 caggtatttg tcaatatcgg agggatttcg ggcttgattc catctacagg agtgactttc
     5341 cccttcttat cccagggtgg aaatagtctt ctagtcttat cagtggcagt agcctttgtc
     5401 ttaaatattg atgccagtga aaaacgcgct aagttgtacc gagaattgga aaatcaacca
     5461 atgaaccttc tgttgaagta ggataaagaa aggatagttt atgtctcttc aaaaattaga
     5521 aaattatagt aataaaagtg ttgtgaaaga agaagtcttg attctaacag aattactgga
     5581 agatattact aaaaatatgc ttgccccaga gacctttgaa aaaataatac agttgaaaga
     5641 attatcaacg caggaagatt atcaaggtct aaaccgtcta gtgactagct tatcaaatga
     5701 tgaaatggtc tatatttcac gctatttctc tatcttacct cttttgatta atatttcaga
     5761 ggatgtggat ttagcttatg aaatcaatca tcaaaataat attgatcagg actatttagg
     5821 taaattatct acaacgatta aattggtagc agaaaaggaa aatgccgttg agatcctaga
     5881 acacttgaat gttgtccctg tgttgacagc ccatccaaca caagtgcaac gcaaaagtat
     5941 gttggattta acaaatcata ttcatagtct tttgcgtaaa taccgtgatg ttaagttggg
     6001 gttaatcaat aaagataaat ggcacaatga tttgcgtcgt tacatcgaaa ttatcatgca
     6061 gacagacatg attcgtgaga aaaaattaaa agtgactaac gaaatcacga atgctatgga
     6121 atattacaac agctcctttt tgaaagctgt ccctcatttg acgacggagt ataagcgctt
     6181 agcgcaagcg catggtctga atttaaaaca ggctaaacca atcaccatgg gtatgtggat
     6241 aggtggtgac cgtgatggaa atccatttgt tacagcaaag accttgaagc agtctgcact
     6301 cactcagtgt gaagtcatca tgaactacta tgataaaaag atttaccaac tttatcgtga
     6361 attttctctt tcaactagca ttgtcaacgt cagcaagcaa gtcagagaaa tggctcgtca
     6421 atccaaggat aactcgattt accgcgaaaa agagctttac cgtcgtgcct tgtttgatat
     6481 tcaatcaaaa attcaggcaa ctaaaaccta tctgattgag gatgaagaag ttgggactcg
     6541 ttatgaaacc gccaatgatt tctacaagga tttgattgcc attcgagatt ctctactaga
     6601 aaataagggt gagtccttga tttcaggtga ttttgtggaa ttattgcagg cagtagagat
     6661 atttggtttt tacttagcgt caattgatat gcgacaagac tctagcgtct atgaagcctg
     6721 tgtggcagaa ctcttgaaat cagcaggaat tcattctcgt tatagcgagt tgagcgaaga
     6781 agaaaagtgt gaccttctct tgaaagaatt agaagaagat ccccgaattc tttctgcgac
     6841 tcacgcagaa aaatcagaat tattagcaaa agaattagct atttttaaga cggctcgtgt
     6901 tttgaaagat aagttgggag atgatgtcat ccgtcagacc atcatttcac atgcaaccag
     6961 cctttctgat atgctagaat tagctattct gttaaaagaa gtaggactgg tggatacgga
     7021 aagggcgcgt gttcagattg ttcccctttt tgaaacaatt gaagacttgg atcattcaga
     7081 ggaaacaatg agaaaatatc tttctcttag ccttgccaaa aaatggattg actcacgaaa
     7141 taactaccaa gaaatcatgc ttggctactc tgacagtaat aaagatggcg gttacttgtc
     7201 atcatgttgg accctctaca aggctcaaca acaattgact gctattggag atgaatttgg
     7261 cgttaaggtt accttcttcc atggtcgtgg tggtactgtc ggtcgtggtg gtgggccaac
     7321 ctatgaagcc attacatctc aaccgctcaa gtctatcaag gatcgtatcc gcttgacgga
     7381 gcagggtgaa gtaattggga ataaatacgg taacaaagac gccgcttact ataaccttga
     7441 aatgctagta tcggcagcta ttaaccgtat gattactcag aagaagagcg ataccaatac
     7501 cccaaatcgt tatgaagcca ttatggatca agtagtggac cgtagttacg atatctaccg
     7561 tgatttggtc tttggtaatg agcatttcta tgattatttc ttcgagtcaa gtccaatcaa
     7621 ggctatttca agttttaata ttggttctcg tccagccgct cgtaagacta ttactgaaat
     7681 cggtggattg cgtgccatcc cttgggtatt ctcatggtca cagagtcgtg ttatgttccc
     7741 tggatggtac ggggttggtt caagcttcaa ggaatttatc aataaaaatc cagagaatat
     7801 tgctatctta cgagatatgt accaaaattg gcctttcttc caatcgcttc tttcaaatgt
     7861 tgatatggtt ttgtcaaaat caaatatgaa tattgctttt gaatatgcta aactttgtga
     7921 agacgagcaa gttaaggcca tctatgagac tattttaaat gaatggcaag ttactaagaa
     7981 cgttatcttg gctattgaag gacatgacga actcttagct gacaatccat atctaaaagc
     8041 tagtctggat taccgtatgc cttactttaa tattctcaac tatattcagt tagagttgat
     8101 taaacgccaa cgtcgtggag aattgtccag tgatcaagaa cgattgattc atatcaccat
     8161 caacggaatt gcgacaggat tgcgtaattc aggttgataa ttttcaagag tgaatgctaa
     8221 aagtgaatat caaaaaaatt ctaatagact attgacaagt agtttaaaaa tgatataatt
     8281 taaccattca gaaaagtaat catacaaact ttttagagag tctgtggtag ctgaaaacag
     8341 ataagtggca atgatgaaaa ttgggctgaa tgctatttag aatttgaaat tataaaaatt
     8401 cggtaagcac accttacagt gcatctcgtt attgcgagac tgagcgatag ggaaattccc
     8461 tataattgag gtggtaccgc gcatcgacgt cctcacacaa gttttttgtg tgaggatttt
     8521 tttgatggag gttagtatgg aaagaaaaca atggcgtcgc ttgtttagat aagtgaaata
     8581 tgttaaagga aataaaaagg agaaacagaa tgaaaaataa acgtttaatt ggaattattg
     8641 ctgcattagc agtcttagta gcaggaagct tgatttattc ttcaatgaat aaatcagaag
     8701 ttcagaataa taaggatgag aagaaaataa ccaagattgg tgtgcttcaa tttgtgagcc
     8761 atccatccct tgatttgatt tataaaggga tccaagatgg acttgcagaa gaaggatata
     8821 aagatgatca agttaaaatt gattttatga actcagaagg tgaccaaagt aaggttgcga
     8881 caatgagtaa acaattggtt gcaaatggga atgaccttgt ggttggtatc gcaacaccag
     8941 cagcccaagg gttggctagt gcaacaaaag acctaccggt tatcatggcc gctattacag
     9001 acccaattgg tgctaacttg gttaaagatt tgaaaaaacc aggtggcaac gttacagggg
     9061 tatctgacca caatccagct caacaacaag ttgaactcat caaggctctg acaccgaatg
     9121 tgaaaacaat cggagctctt tactcaagta gcgaagacaa ttcaaaaaca caggtcgaag
     9181 aatttaaggc ttatgctgaa aaagcaggtc tgacagtgga aacatttgca gttccttcaa
     9241 caaatgaaat tgcctcaact gtcactgtta tgactagcaa ggtagatgct atttgggttc
     9301 caattgataa caccattgca tcaggatttc caacggttgt ctctagcaat caaagttcta
     9361 agaaaccaat ttatcccagt gcgacagcta tggtagaagt aggtggtttg gcatcagttg
     9421 taattgacca acatgacctt ggtgtggcaa caggtaaaat gattgtgcaa gtcttgaaag
     9481 gtgcaaaacc agccgatacc ccagtcaatg tcttttcaac tggtaagtca gtcatcaata
     9541 aaaaaatagc acaagaacta ggtattacta ttcctgagtc tgttctcaaa gaagcaggac
     9601 aagtcatcga ataaataagg tagggaggag gagactcttc ccatttttta aaaagatgaa
     9661 tatagaaagg attaaataaa atatgattgt ttccattatt tctcaaggat ttgtctgggc
     9721 tattctaggt ctgggaatct ttatgacatt taggatttta aactttccag atatgacgac
     9781 agaaggttcc ttccctcttg ggggagctgt tgctgtcact ttgataacca aaggcgtgaa
     9841 cccattttta gcgacacttg ttgctgtagg agcaggttgt ttggctggaa tggcagcagg
     9901 ccttctttat acaaaaggga agatcccaac cttgctctca ggaattttgg tgatgacttc
     9961 ttgtcactca atcatgctct tgattatggg acgtgcgaat ttaggcctgc ttggaaccaa
    10021 gcaaattcag gatgttttgc cttttgattc ggatttgaat caactcttga caggtctcat
    10081 ctttgtgagt attgttattg ctctcatgct ctttttcttg gacactaaac tcggacaagc
    10141 ctatattgct acaggggata atcctgatat ggctagaagt ttcgggattc atactggacg
    10201 catggagctc atgggcttgg tcttatcaaa tggtgtgatt gcccttgcag gtgccctcat
    10261 tgctcagcaa gaaggctatg ccgatgtgtc tcgagggatc ggggttatcg ttgtggggct
    10321 tgcaagtttg attattggag aagttatttt caagagtttg agcttggcag agcgtttggt
    10381 tactatcgtt gtaggttcta tcgcttatca atttttagtg tgggcagtta tcgcacttgg
    10441 ctttaataca agttaccttc gtttatacag tgccttgatt ttagcagtct gcctcatgat
    10501 tccaacattt aagcaaacaa tcttgaaagg agccaagtta agcaaatgac agcaattgta
    10561 gaattaaaaa atgcaaccaa aatcgttaaa aatggctttg atgaagaaaa gattatttta
    10621 aatgatgttt ccttagaaat ttttgaacgg gactttatca cgattttggg cggaaatggt
    10681 gctggaaaat caactctctt taacactata gcagggacct tatcactaac tagtggaact
    10741 atccgtattt taggtgaaga tctcactaag ttttcacccg agaagcgtgc caagtacctg
    10801 tctcgtgtct tccaagatcc aaagatgggg acagctcccc gtatgacggt cgctgaaaat
    10861 cttttaatcg ccaagtttcg tggtgaaaag cgtggattgt taccaagacg cttggctagc
    10921 tataaggatg aatttcaggc aaccattgaa aaagtaggaa atggtcttga gaaacacttg
    10981 aatacaccga ttgagttctt atcaggtgga caaagacagg ctttgagtct cttgatggca
    11041 accttgaagc gacctgaatt actcctgtta gatgagcata ctgctgccct ggatccaaag
    11101 actagtgttg ctttgatgga attgacagat gaatttgtta agaaagatca gctaacagcc
    11161 cttatgatta ctcatcatat ggaagatgct ctcaaatacg gcaatcgctt gattgtcatg
    11221 aaagaaggac ggattatcca agatttaaac caagaagaaa aagcaaaaat gaaaatctct
    11281 gattattatc aactctttga ataaagtagg gaaaatgagt gaaatggttt actttttttc
    11341 tgaaataaag tatactatat aaagtaaact atgataacat ggaggtattg tgtatggttg
    11401 acaaacaagt cattgaagaa atcaaaaaca atgccaacat tgtggaagtc ataggagatg
    11461 tgatttcttt acaaaaggca ggacggaact atctagggct ctgtcctttt catggtgaaa
    11521 aaacaccttc tttcaacgtt gtagaggaca agcagtttta ccactgtttt ggttgtggtc
    11581 gctcaggtga tgtctttaaa ttcatcgagg agtaccaagg ggttaccttt atggaggctg
    11641 tccaaatctt aggtcagcgt gtcgggattg aggttgaaaa accgctttat agtgaacaga
    11701 agccagcctc gcctcaccaa gctctttatg atatgcacga agatgcggct aaattttacc
    11761 atgctattct catgacaacg actatgggcg aagaggccag aaattacctt tatcagcggg
    11821 gtttgacaga tgaagtgctt aaacattttt ggattggttt agcacctcca gaacgaaact
    11881 atctctatca acgtttgtct gatcagtatc gtgaagaaga tttactggat tcaggcctgt
    11941 tttatctctc ggatgccaat caatttgtag acacctttca caatcgcatt atgtttcccc
    12001 tgacaaatga ccaaggaaag gtcattgcct tctcaggtcg tatctggcaa aaaacagatt
    12061 cacaaacttc taagtataaa aacagccgat cgactgcaat ttttaacaaa agttacgaat
    12121 tatatcatat ggatagggca aaaaaatctt ctggaaaaac tagtgagatt tacctgatgg
    12181 aaggattcat ggatgttatt gcagcctatc aggctggaat cgaaaatgct gtggcgtcga
    12241 tgggaacggc cttgagtcga gagcatgttg agcatctgaa aaggttaacc aagaaattgg
    12301 ttcttgttta cgatggagat aaggctgggc aagccgcgat attgaaagca ttggatgaaa
    12361 ttggtgatat gcctgtgcaa atcgttagca tgcctgataa cttggatcct gatgagtatc
    12421 tacaaaaaaa tggtccagaa gacttggcct atctattaac gaaaactcgt attagtccga
    12481 ttgagttcta cattcatcag tacaaacctg aaaacagtga aaatctgcag gctcagattg
    12541 agtttattga aaaaatagct cccttgattg ttcaagaaaa gtccatcgct gctcaaaaca
    12601 gctatattca tattttagct gacagtctgg cgtcctttga ttatgcccag attgagcaga
    12661 ttgttaatga gagtcgtcag gtgcaaaggc agaatcgcat ggaaggaatt tccagaccga
    12721 cgccaatcac catgcctatc accaagcagt tatcggctat tatgagggca gaagcccatc
    12781 tactctatcg gatgatggaa tcccctcttg ttttgaacga ttatcgtttg cgagaagact
    12841 ttacatttgc tacacctgaa tttcaggtct tatatgactt gcttggccag tatggaaatc
    12901 ttcctccaga agttttagca gagcagacag aggaagttga aagagcttgg taccaagttt
    12961 tagctcagga tttgcctgct gagatatcgc cgcaggaact tagtgaagta gagatgactc
    13021 gaaacaaggc tctcttgaat caggacaata tgagaatcaa aaagaaggtg caggaagcta
    13081 gccatgtagg agatacagat acagccctag aagaattgga acgtttaatt tcccaaaaga
    13141 gaagaatgga gtaataatgg caacaaaaca aaaagaagta acaacatttg acgtacaggt
    13201 agcagaattt atccgtaatc ataagcaaaa agggacagca acagatgatg aaatcaatgc
    13261 aagtctggtt attcctttta ccttggacgc tgatgggatt gaagatctct tgcaacggat
    13321 tcaggatgca ggcatttcta tcacagataa cgaaggaaat ccaagtgcgc gtgttcttag
    13381 caatgaagaa gaaccagaac tcagcgatga ggacttgatt gggtcaactt ctgctaaggt
    13441 caatgaccct gtccgtatgt acttgaaaga aataggggtc gttcctctct tgaccaatga
    13501 agaggagaaa gagttggcac tggctgttga agctggcgat atcgaagcca aacaacgtct
    13561 tgcggaagcc aatcttcgtt tggttgtttc tattgccaaa cgctatgtcg gtcgtggcat
    13621 gcagttcctt gacttgattc aagaaggaaa tatgggcttg atgaaggcgg ttgacaagtt
    13681 tgactattct aaagggttca agttttcaac ttatgcaact tggtggattc gtcaggctat
    13741 cactcgtgct attgcggatc aagctcgtac catccgtatc ccagttcaca tggttgaaac
    13801 tatcaataaa ttggttcgtg aacagcggaa tctccttcaa gaattggggc aagatccgac
    13861 accagaacag attgctgaac gaatggatat gacacctgat aaggttcgtg aaatcttgaa
    13921 gattgcccaa gaaccagtat ctcttgaaac tcctatcggt gaagaggacg atagccacct
    13981 tggagacttt atcgaagatg aagtgattga aaatccagtg gattatacga ctcgtatcgt
    14041 cttgcgtgag caattggatg aaatcttaga tactcttaca gaccgtgaag aaaatgttct
    14101 gcgtctacgt tttggactag atgatggaaa aatgcgcaca cttgaagatg tggggaaagt
    14161 ctttaacgta actcgtgagc gtatccgtca gattgaagca aaggctttga gaaaactacg
    14221 ccaaccaagc cgcagcaaac cgcttcgtga ttttattgaa gactaagagt gaggaaaata
    14281 tggcttatac agaagagcaa attgaaaaca tcaaaacacg gattttaaca gccttggaag
    14341 aagtcatcga ccctgagttg ggaatcgata ttgtcaatct tggtttgatc tatgagattc
    14401 gttttgacgg tgacacaggg caaacagaga ttgacatgac tttgacaact atgggttgtc
    14461 ccttagcaga ccttttgaca gaccagattt atgatgctat gatagaggtt ccagaagtaa
    14521 cggatactga ggttaaatta gtctggtatc cagcctggac agttgaaaaa atgagtcgct
    14581 atgcccgcat tgccctaggc attaagtaaa catataaaag catgtgagag actgttggaa
    14641 aagcgaggaa atttcccctc ttttcctcta gtctctcctt tcttttgctg attttattca
    14701 aagaaaatga tataatagta gttatggaga aaaagaaatt acgcatcaat atgttgagtt
    14761 caagtgagaa agtagcagga cagggagttt caggtgctta ccgtgaatta gttcgtcttc
    14821 ttcaccgtgc tgccaaggac caattgattg ttacagaaaa tcttccaatc gaggcagatg
    14881 tgactcactt tcatacgatt gattttccct attatttatc aaccttccaa aagaaacgct
    14941 cagggagaaa gattggctat gtgcatttct tgccagctac acttgaagga agtttgaaaa
    15001 ttccattttt cttaaaggga attgtgaaac gctatgtatt ttctttttac aaccggatgg
    15061 agcacttggt tgtggtcaat cctatgttta ttgaggattt ggtagtagct ggtattccac
    15121 gtgaaaaagt gacctatatt cctaactttg tcaacaagga aaaatggcat cctctaccac
    15181 aagaagaggt agtcagactg cgcacagatc ttggtcttag tgacaatcag tttatcgtag
    15241 taggtgctgg gcaagttcag aaacgtaaag ggattgatga ctttatccgt ctggctgagg
    15301 aattgcctca gattaccttt atctgggctg gtggcttctc ttttggtggt atgacagatg
    15361 gttatgaaca ctataagaaa attatggaaa atccccctaa aaatttgatt tttccaggca
    15421 ttgtatcgcc agagcggatg cgcgaattgt atgctctagc ggatcttttc ttgttgccta
    15481 gttacaatga gctctttcct atgactattt tagaagctgc gagttgtgag gctcctatta
    15541 tgttgcgtga tttagatctc tataaggtga ttttggaggg aaattatcgg gcgacagcgg
    15601 gtagagaaga gatgaaagag gctattttgg aatatcaagc aaatcctgct gtcttaaaag
    15661 atctcaaaga aaaggctaag aatatttcca gagagtattc tgaagagcat ctgttacaaa
    15721 tctggttgga cttttatgag aaacaagccg ctttagggag aaagtaaaaa gtgaggtaat
    15781 ctatgcgaat tggtttattt acagatacct attttcctca ggtttctggt gttgcgacca
    15841 gtattcgaac cttgaaaaca gaacttgaaa agcagggaca tgctgttttt atctttacga
    15901 cgacagataa ggatgtcaat cgctacgaag attggcaaat tatccgcatt ccaagtgttc
    15961 ctttctttgc ttttaaggat cgtcgctttg cctaccgagg ttttagcaag gcacttgaaa
    16021 ttgctaaaca gtatcagcta gatattatcc atactcagac agaattttct cttggcctgt
    16081 tggggatttg gattgcgcgt gaattgaaaa ttccagtcat ccatacctat cacacccagt
    16141 atgaagacta tgtccattat attgctaagg ggatgttgat ccggccgagt atggtcaagt
    16201 atctggttag aggtttcctg catgatgtgg atggggttat ttgccctagt gagattgtcc
    16261 gtgacttgct atctgattat aaggtcaagg ttgaaaaacg ggtcattcct actgggattg
    16321 aattagccaa gtttgagcgt ccggaaatca agcaggaaaa tttgaaagaa ctgcgtagta
    16381 aactagggat tcaagatggt gaaaagacgt tgcttagtct ttcgagaatc tcctatgaaa
    16441 aaaatattca agcagtttta gcagcctttg ctgatgttct gaaagaggaa gacaaggtta
    16501 aactggtagt agctggggat ggcccttatc tgaatgacct caaagagcaa gcccagaacc
    16561 tagagattca agactcagtc atctttacag ggatgattgc tcctagtgag acggctcttt
    16621 actataaagc ggcggatttc ttcatttcgg catcgacaag cgaaacgcaa ggtttgacct
    16681 acttggaaag cttagccagt ggaacacctg tcattgctca cggaaatcct tatttgaaca
    16741 acctcatcag tgataaaatg tttggaacct tgtactatgg agaacatgat ttggctggtg
    16801 ctattttgga agccctgatt gcaacaccag acatgaacga gcatacctta tcagagaaat
    16861 tgtatgagat ttcagctgag aactttggga aacgagtgca tgagttttat ctggatgcca
    16921 ttatttcaaa taacttccag aaagatttgg ctaaagatga tacggtcagt cagcgtatct
    16981 ttaagacagt tttgtatctt cagcaacagg tggttgctgt acctgtaaaa ggatctagac
    17041 gcatgttgaa ggcttcaaaa acacagttga tcagtatgag agactattgg aaagaccatg
    17101 aagaatagaa agaggaacag ctatgaaaaa aacaattaat gagaagcggt cgtgataaaa
    17161 agattgcggg tgtttgtgct ggggtggccc attatctgga tatggatccg actatcgttc
    17221 aagtcatttg gggtgttctt acttgctgtt acggagctgg aattgtagct tacattattt
    17281 tatggattat cgcaccagta gcaactgact attgaaaact agctcaattc atgctaagat
    17341 aatagaaaat agaatgtaac gaaggagaga aaaatggcat ttggagataa tggaaatcgt
    17401 aaaaaaacta tgtttgagaa aataaccttg tttatcgtga ttatcatgct agtagcaagt
    17461 ttattgggaa tttttgcaac tgcaattggt gccctcagta atctataaaa tagattcaag
    17521 aaaatttagt gactgggatt tcccagccct tttttaaagt gagaagaaat aatgagtatg
    17581 tttttagata cagctaagat taaggtcaag gctggtaatg gtggcgatgg tatggttgcc
    17641 tttcgtcgtg aaaaatatgt ccctaatgga ggcccttggg gtggtgatgg tggtcgtgga
    17701 ggcaatgtgg tcttcgttgt agacgaagga ctacgtacct tgatggattt ccgctacaat
    17761 cgtcatttca aggctgattc tggtgaaaaa gggatgacca aagggatgca tggtcgtggt
    17821 gctgaggacc ttagagttcg agtaccacaa ggtacgactg ttcgtgatgc ggagactggc
    17881 aaggttttaa cagatttgat tgaacatggg caagaattta tcgttgccca cggtggtcgt
    17941 ggtggacgtg gaaatattcg tttcgcgaca ccaaaaaatc ctgcaccgga aatctctgaa
    18001 aatggagaac caggtcagga acgtgagtta caattggaac taaaaatctt ggcagatgtc
    18061 ggtttagtag gattcccatc tgtagggaag tcaacacttt taagtgttat tacctcagct
    18121 aagcctaaaa ttggtgccta ccactttacc actattgtac caaatttagg tatggttcgc
    18181 acccaatcag gtgaatcctt tgcagtagcc gacttgccag gtttgattga aggggctagt
    18241 caaggtgttg gtttgggaac tcagttcctc cgtcacatcg agcgtacacg tgttatcctt
    18301 cacatcattg atatgtcagc tagcgagggc cgtgatccat atgaggacta cctagctatc
    18361 aataaagagc tggagtctta caatcttcgc ctcatggagc gtccacagat tattgtagct
    18421 aataagatgg acatgcctga gagtcaggaa aatcttgaag actttaagaa aaaattggct
    18481 gaaaattatg atgaatttga agagttacca gctatcttcc caatttctgg attgaccaag
    18541 caaggtctgg caacactttt agatgctaca gctgaattgt tagacaagac accagaattt
    18601 ttgctctacg acgagtccga tatggaagaa gaagcttact atggatttga cgaagaagaa
    18661 aaagcctttg aaattagtcg tgatgacgat gcgacatggg tactttctgg tgaaaaactc
    18721 atgaaactct ttaatatgac caactttgat cgtgatgaat ctgtcatgaa atttgcccgt
    18781 cagcttcgtg gtatgggggt tgatgaagcc cttcgtgcgc gtggagctaa agatggggat
    18841 ttggtccgca ttggtaaatt tgagtttgaa tttgtagact aggagactgg tatgggagat
    18901 aaaccgatat ctttccgaga tgcggatggt aattttgttt ccgccgcaga cgtttggaat
    18961 gaaaagaaat tggaagaact atttaatcgt ctcaatccaa atcgtgcctt gagattggca
    19021 cgaactaaaa aggaaaatcc atctcagtaa agaagctaaa aaatcccgtg cctcatcaga
    19081 cacgggattt tgtggtacga caggcatgta tagcaaactg aatctggaat agcacagcat
    19141 atcttctaaa atatagtaaa atgaaatgag aacaggacaa atcgatcagg acagtaaaat
    19201 cgatttctaa
//
LOCUS       DAVKAO010000032        18692 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKAO010000032 DAVKAO010000000
VERSION     DAVKAO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.032"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            963..2222
                     /locus_tag="VWX16_001211"
     CDS             963..2222
                     /locus_tag="VWX16_001211"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9158565.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2238..2789
                     /locus_tag="VWX16_001212"
     CDS             2238..2789
                     /locus_tag="VWX16_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9158566.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2782..4059
                     /locus_tag="VWX16_001213"
     CDS             2782..4059
                     /locus_tag="VWX16_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9158567.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            4075..4935
                     /locus_tag="VWX16_001214"
     CDS             4075..4935
                     /locus_tag="VWX16_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9158568.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5316..6120
                     /locus_tag="VWX16_001215"
     CDS             join(5316..5684,5686..6120)
                     /locus_tag="VWX16_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9158569.1"
                     /translation="MNDEASKQLTDTRFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEATVMIDATEVQINRPKKQLANYSGKKKFHAMKAQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKFKSLTVEDKTYNHA
                     LSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            6311..8602
                     /gene="pcrA"
                     /locus_tag="VWX16_001216"
     CDS             6311..8602
                     /gene="pcrA"
                     /locus_tag="VWX16_001216"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9158570.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            8645..9325
                     /gene="radC"
                     /locus_tag="VWX16_001217"
     CDS             8645..9325
                     /gene="radC"
                     /locus_tag="VWX16_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9158571.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9322..10011)
                     /locus_tag="VWX16_001218"
     CDS             complement(9322..10011)
                     /locus_tag="VWX16_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9158572.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(10029..10670)
                     /locus_tag="VWX16_001219"
     CDS             complement(10029..10670)
                     /locus_tag="VWX16_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9158573.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(10753..10968)
                     /locus_tag="VWX16_001220"
     CDS             complement(10753..10968)
                     /locus_tag="VWX16_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9158574.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(10976..11323)
                     /locus_tag="VWX16_001221"
     CDS             complement(10976..11323)
                     /locus_tag="VWX16_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9158575.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(11328..12443)
                     /locus_tag="VWX16_001222"
     CDS             complement(11328..12443)
                     /locus_tag="VWX16_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9158576.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(12453..13412)
                     /locus_tag="VWX16_001223"
     CDS             complement(12453..13412)
                     /locus_tag="VWX16_001223"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9158577.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(13525..14094)
                     /locus_tag="VWX16_001224"
     CDS             complement(13525..14094)
                     /locus_tag="VWX16_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9158578.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            14222..14893
                     /locus_tag="VWX16_001225"
     CDS             14222..14893
                     /locus_tag="VWX16_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9158579.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            14877..15695
                     /locus_tag="VWX16_001226"
     CDS             14877..15695
                     /locus_tag="VWX16_001226"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9158580.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            15692..16588
                     /locus_tag="VWX16_001227"
     CDS             15692..16588
                     /locus_tag="VWX16_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9158581.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            16632..17606
                     /gene="pta"
                     /locus_tag="VWX16_001228"
     CDS             16632..17606
                     /gene="pta"
                     /locus_tag="VWX16_001228"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9158582.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(17922..18692)
                     /locus_tag="VWX16_001229"
                     /pseudo
     CDS             complement(17922..18692)
                     /locus_tag="VWX16_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5548 a         3395 c         4081 g         5668 t
ORIGIN      
        1 tgtactattc tagtttcaat caactatatt gttataaatt gatttgaatt tcaaaattaa
       61 attgtttgat tcttatttca atttgttata gtatatctga tgtcaaagtt ctcggcgagt
      121 caaatagcga ttcccaagcc tgactatcgt gaggtagcgg attaaaatgg tctggggata
      181 gaccgtttta agtctgacgc tggaaataag aattgtcaga agaagggata gcgaaatcgt
      241 ggctctacga acaggaacgt gataataagg cgtatatagc ggataagagg gcatcaaact
      301 ctaaagtcca aaaaggtagt cgtaacctat atgcgtaaat cacgagagta attgaattcg
      361 tactaagatt ttctattttc actgtaacct tttaacgccc ttatatcttg tatacacgag
      421 gaaagatgta cgacttatcc cgtgaggtct atcactataa agagaaaacg acagatagaa
      481 gtgatcctga gtcacggtta tctgtctgat aggacggtat gtataaaacg cttctgtgaa
      541 ctgagagaag ggggagaagt tcttgctaaa atttagttga acagccgtat tccgatactt
      601 agataagaga tctagtctta gctcctactc agttttaggg gataaaaaag gggcaatagc
      661 gattcgagaa agattatact cttcgaaaat ctcttcaaat cacgtcaata tcgccttgtc
      721 gtatgtgtag gatactgact acgtcagttc catctacaac ctcaaaacag tgttttgagc
      781 aacctgcggc tagtttccta gtttgatctt tgattttcat tgagtattag taattcagtt
      841 actaactcgt caactctgat ttatccaata aaattgaaaa ggatggaaaa aaggataaat
      901 ttatgatata ctttattttg aagaccttat tagaaatctt gaaagagtat tgaaaactta
      961 gaatgagaaa aattgttatc aatggtggat taccactgca aggtgaaatc actattagtg
     1021 gtgctaaaaa tagtgtcgtt gccttaattc cagctattat cttggctgat gatgtggtga
     1081 ctttggattg cgttccagat atttcggatg tagccagtct tgtcgaaatc atggaattga
     1141 tgggagctac tgttaagcgt tatgacgatg tattggagat tgacccaaga ggtgttcaaa
     1201 atattccaat gccttatggt aaaattaaca gtcttcgtgc atcttactat ttttatggga
     1261 gcctcttagg ccgttttggt gaagcgacag ttggtctacc gggaggatgt gatcttggtc
     1321 ctcgtccgat tgacttacac cttaaggcgt ttgaagctat gggtgccact gctagctacg
     1381 agggagataa catgaagtta tctgctaaag atacaggact tcatggtgca agtatttaca
     1441 tggatacggt tagtgtggga gcaacgatta atacgatgat tgctgcggtt aaagcaaatg
     1501 gtcgtactat tattgaaaat gcagcccgtg aacctgagat tattgatgta gctactctct
     1561 tgaataatat gggcgcccat atccgtgggg caggaactaa tatcatcatt attgatggtg
     1621 ttgaaagatt acatgggaca cgtcatcagg tgattccaga ccgcattgaa gctggaacat
     1681 atatatcttt agctgctgca gttggtaaag gaattcgtat aaataatgtt ctttacgaac
     1741 acctggaagg gtttattgct aagttggaag aaatgggagt gagaatgact gtatctgaag
     1801 acagcatttt tgtcgaggaa cagtctaatt tgaaagcaat caatattaag acagctcctt
     1861 acccaggctt tgcaactgat ttgcaacaac cgcttacccc tcttttacta agagcgaatg
     1921 gtcgtggtac aattgtcgat acgatttacg aaaaacgtgt aaatcatgtt tttgaactag
     1981 caaagatgga tgcggatatt tcgacaacaa atggtcatat tttgtacacg ggtggacgtg
     2041 atttacgtgg ggccagtgtt aaagcgaccg acttaagagc tggggctgca ctagtcattg
     2101 ctgggcttat ggctgaaggt aaaactgaaa ttaccaatat cgagtttatc ttacgtggtt
     2161 attctgatat tatcgaaaaa ttacgtaatt taggagcgga tattagactt gttgaggatt
     2221 aaaccgtaga ggtgtttatg aatatttgga ccaaattagc aatgttttct ttttttgaaa
     2281 cggatcgctt gtatttgcgt cctttctttt ttagtgatag tcaggacttc cgcgagatag
     2341 cttcaaatcc agaaaatctt caatttattt tcccaacgca ggcaagtctg gaagaaagtc
     2401 aatatgcact ggccaattac tttatgaagt cccctttggg agtgtgggca atttgtgacc
     2461 agaaaaatca acaaatgatt ggttctatta aatttgagaa gttagatgaa atcaaaaaag
     2521 aagctgagct tggctatttt ttgagaaaag atgcttggtc gcaaggattt atgacagagg
     2581 ttgttagaaa aatttgtcag ctttcttttg aggaatttgg cttaaaacaa ttatctatca
     2641 ttacccacct tgaaaatgaa gctagccaaa gagttgctct taagtctgga tttagtttgt
     2701 tccgtcagtt taagggaagt gatcgttaca caagaaaaat gcgggattat cttgaatttc
     2761 ggtatgtaaa aggagagttc aatgagtaag catcaggaaa ttctaagcta tttggaggaa
     2821 ttaccagtag gtaaaagggt cagtgttcgt agcatttcga atcatctagg agttagtgat
     2881 ggaacagcct atcgagctat taaagaagct gaaaaccgtg gaattgtgga gacccgtcct
     2941 agaagtggaa caattcgtgt taaatcccag aaagttgcta tagagagatt aacgtttgct
     3001 gaaattgcag aagtgacctc ttctgaggtt ctggctgggc aagaaggttt agagagagag
     3061 tttagtaagt tttcaattgg tgccatgact gaacaaaata tcttgtctta ccttcatgat
     3121 ggggggctct tgattgtcgg agaccgaacc cgtattcagt tgctagcctt ggaaaatgaa
     3181 aatgcagttc tggttacagg gggatttcag gttcatgatg atgtgcttaa actggccaat
     3241 caaaaaggga ttcctgttct aagaagtaag catgatacct ttaccgtcgc gaccatgatc
     3301 aataaagcct tgtcaaatgt ccaaatcaag actgatattc tgacagttga gaaactttat
     3361 cgccctagtc atgagtatgg ttttctgaga gagacagata cagttaaaga ttatttggac
     3421 ttggttcgta agaatcgtag cagccgtttc cctgttatca atcaacatca ggtcgttgtt
     3481 ggcgttgtaa ccatgagaga cgctggtgat aaatcaccaa gcacgacaat tgataaggtt
     3541 atgtctcgta gtctattttt ggttggatta tcgacaaata ttgccaatgt gagtcaacgg
     3601 atgatcgcag aagactttga aatggtacca gttgttcgaa gcaatcaaac tttgcttggc
     3661 gttgtgacgc gacgagatgt catggagaag atgagccgtt cccaagtttc ggctctacca
     3721 actttttctg agcagattgg acaaaagctc tcttatcacc atgatgaagt agtcattaca
     3781 gtggaaccct ttatgctaga aaaaaatgga gttttggcta atggtgtatt ggcagaaatt
     3841 ctgacccaca tgacccaaga tttagttgtt aatagtggtc gcaatctcat tatcgagcag
     3901 atgctgatct actttttgca ggctgttcag atagatgata tattgcgcat tcaggcacgg
     3961 attattcatc atacgagacg gtcagctata attgattacg atatttatca tggtcaccag
     4021 attgtttcaa aagcaaatgt gactgttaaa attaattaga aactaggaga aaagatgata
     4081 acattaaaat cagctcgtga aatcgaagct atggacaagg ctggtgattt tctagcaagt
     4141 attcatatag gcttacgtga tttgattaaa ccaggcgtag atatgtggga agttgaagaa
     4201 tatgtccgcc gtcgttgtaa agaagaaaat ttccttccac ttcagattgg ggttgacggt
     4261 gccatgatgg actatcctta tgctacctgt tgctctctta acgatgaagt ggctcacgct
     4321 ttccctcgtc attatatctt gaaagatggt gatttgctca aagttgatat ggttttggga
     4381 ggtcccattg ctaaatctga cctgaatgtc tcaaaattaa acttcaacaa tgttgaacaa
     4441 atgaaaaaat acactcagag ctattctggt ggtttagcag actcatgttg ggcttatgct
     4501 gttggtacac cgtccgaaga agtcaaaaac ttgatggatg taaccaaaga agctatgtac
     4561 aagggtattg agcaagctgt tgttggaaat cgtatcggtg atatcggtgc ggctattcaa
     4621 gaatacgctg aaagtcgtgg ttacggtgta gtgcgtgatt tggttggtca tggtgttggc
     4681 ccaactatgc acgaagaacc aatggttcct aactatggta ttgcaggtcg tggactccgt
     4741 cttcgtgaag gaatggtctt aaccattgaa ccaatgatca atacaggcga ttgggaaatt
     4801 gatacagata tgaaaactgg ttgggcgcat aagaccattg acggtggatt gtcatgtcag
     4861 tatgaacacc aatttgtcat tacgaaagat ggacctgtta tcttgactag ccaaggtgaa
     4921 gaaggaactt attaataaaa agtgaaaaga ctactggaag tttattttga taaaaaatcc
     4981 agtagatctt ttcataataa aacgcattgt atcaagtgtt aggggctgat atcatgcgtt
     5041 tttctgcttt taagattttt tccaactctg tttgtaagcg catcataaca aagggtctag
     5101 gattcagggc tctcctccta tatactatta gtaaagtaaa actaagggag gatattttag
     5161 tgtcgcagtt tattgttcct gtagagattc cacaatattg tcgttttgat tctaaaaaga
     5221 gaaatggaat tctgtttaac gttcgtattg ccaatcttaa atttaccttt tttagattag
     5281 acttcctgcg aaacaaaata tggtatagta gttctatgaa tgatgaagca agtaaacaat
     5341 taactgatac acgatttaag cgtcttgttg gtgttcagcg taccactttt gaagagatgt
     5401 tagctgtatt aaaaacagct tatcaactta aacacgcaaa aggtggacga aaacctaaat
     5461 taagcctaga agaccttctt atggccactc ttcaatatgt gcgagaatac cgcacttatg
     5521 aagaaattgc ggctgatttt ggtattcacg aaagcaactt actccgtcgg agccaatggg
     5581 ttgaagtaac tcttgttcaa agtggtgtta cgatttcaag aactcctctc agttctgagg
     5641 ccacggtaat gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa acaattagcg
     5701 aattattctg gtaaaaagaa atttcacgct atgaaggctc aagcgattgt cacaagtcaa
     5761 gggagaattg tttctttgga tatcactgtg aactattgtc atgatatgaa gttgttcaaa
     5821 atgagtcgca gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg
     5881 ctcatgaaga tatatcctca agcacaaact ccacgtaaat ccagcaaatt caagtcgcta
     5941 acagttgaag ataaaaccta taaccatgcg ctatctaagg agagaagcaa ggttgagaac
     6001 atctttgcca aagtaaaaac gtttaaaata ttttcaacaa cctatcgaaa tcatcgtaaa
     6061 cgcttcggat tacgaatgaa tttgagtgct ggtattatca atcatgaact aggattctag
     6121 ttttgcagga agtctattga ggtattgagc tagtttatga aaaaattggg tgaaaagtcg
     6181 agtgttttag aaacccacag tgtagtattc tagtttcaat ccactatatt ttgctactcc
     6241 ccgtaaagtt tctattttcc ctgatttctg atataataga aatattgact tcaagagtaa
     6301 ggaagagaag atgaacgcat tattaaatgg aatgaatgac cgtcaggctg aggcggtgca
     6361 aacgacagaa ggtcccttgc taatcatggc aggggctggt tctggaaaga ctcgtgtttt
     6421 gacccaccgt atcgcttatt tgattgatga aaagctggtc aatccttgga atatcttggc
     6481 cattaccttt accaacaagg ctgcgcgtga gatgaaagag cgtgcttata gcctcaatcc
     6541 agcgactcag gactgtctga ttgcgacctt ccactccatg tgtgtgcgta ttttgcgtcg
     6601 cgatgcggac catattggct acaatcgtaa ttttacaatt gtggatcctg gtgaacagcg
     6661 aacgctcatg aaacgtattc tcaaacagtt gaacttggac cctaaaaaat ggaatgaacg
     6721 aactattttg gggaccattt ccaatgctaa gaatgatttg attgatgatg ttgcttatgc
     6781 tgcccaagct ggcgatatgt atacgcaaat tgtggcccag tgttatacag cctatcaaaa
     6841 agaacttcgt cagtctgaat ccgttgactt tgatgatttg attatgctga ccttgcgtct
     6901 ctttgatcaa aatcctgatg ttttgaccta ctaccagcaa aaattccaat acatccacgt
     6961 tgatgagtac caagatacca accacgctca gtaccaattg gtcaaactct tggcttcccg
     7021 ttttaaaaat atctgtgtgg ttggggatgc ggaccagtct atctacggtt ggcgtggtgc
     7081 tgatatgcag aatatcttgg actttgaaaa ggattacccc aaagccaagg ttgttttgtt
     7141 ggaggaaaat taccgctcaa ccaaaaccat tctccaagcg gccaacgagg ttattaaaaa
     7201 taataaaaat cgccgtccta aaaatctctg gactcaaaac gctgatgggg agcaaatcgt
     7261 ttactatcgt gccgatgatg agctggatga ggctgtattt gtagccagaa ccatcgatga
     7321 acttagtcgc agtcaaaact tccttcataa ggattttgca gttctctatc ggactaatgc
     7381 ccagtcccgt acaattgagg aagccctgct caagtctaac attccttata ccatggttgg
     7441 cggaaccaaa ttctacagcc gtaaggaaat tcgcgatatt attgcttatc tcaaccttat
     7501 tgctaatttg agtgacaata ttagttttga gcgtattatc aacgagccta aacgtggaat
     7561 tggtctaggt acagttgaga aaatccgtga ttttgcaaat ttgcaaaata tgtctatgct
     7621 ggatgcttct gctaatatta tgttgtctgg tatcaagggt aaggcagccc aatctatctg
     7681 ggattttgcc aatatgatgc ttgatttgcg ggagcagcta gaccacttaa gcattacaga
     7741 gttggttgag tccgtcctag aaaaaacagg ttatgtcgat attcttaacg cccaagcgac
     7801 tctagaaagc aaggcacggg ttgaaaatat cgaagagttt ctttctgtta cgaagaactt
     7861 tgatgacacc acggatgtga cagaagagga aactggtctg gacaaactga gtcgtttctt
     7921 aaatgacttg gctttgattg ccgacacaga ttcaggtagt caggagacat cagaagtgac
     7981 cttgatgacc ctgcatgctg ccaaaggtct cgaatttcca gttgtctttt tgattgggat
     8041 ggaagaaaat gtctttccac ttagtcgtgc gactgaagat tcagatgaat tagaagaaga
     8101 gcgccgtcta gcctatgtag gtatcacgcg tgcagagaaa attctctatc tgaccaatgc
     8161 caactcacgc ttgctttttg gtcgtaccaa ttataaccgt ccgactcgtt ttattaacga
     8221 aatcagttca gacttgcttg agtatcaagg tctggctcgt cctgcaaata caagctttaa
     8281 ggcatcatat agcagtggta gtatttcctt tggtcaaggt atgagtttgg ctcaggctct
     8341 tcaagaccgt aaacgcggtg ctgccccaaa atcaatccag tcaagcggtc ttccatttgg
     8401 tcaatttaca gctggcgcaa aaccagcatc tagcgaggca aattggtcca ttggtgatat
     8461 tgctctccac aagaaatggg gagagggaac cgttctggaa gtttcaggta gcggtgctag
     8521 gcaggaattg aaaatcaatt tcccagaagt aggtttgaaa aaacttttag ccagtgtggc
     8581 tccaattgag aaaaaaatct aattttccat ccttctcacg aataataaag tgaggaggat
     8641 ttttatgtac agtatttcat tccaagaaga ttcactatta ccaagagaaa ggctggccaa
     8701 ggaaggagtt gaagcgctta gtaaccaaga gttgctagct attttactca ggacaggaac
     8761 acgtcaagct agcgtttttg aaattgccca aaaagtcttg aacaatcttt caagcctaac
     8821 ggatttgaaa aaaatgaccc tgcaggaatt gcagagtttg tctggtattg ggcgtgttaa
     8881 ggccatagaa ttacaagcta tgattgaact ggggcatcgt attcacaaac acgagactct
     8941 tgaaatggaa agtattctca gcagtcaaaa gttggccaag aagatgcagc aggaattagg
     9001 ggataaaaaa caagagcacc tggtggcact ctatctcaat actcaaaatc aaatcatcca
     9061 tcagcagacc atttttatcg ggtctgtaac tcgtagtatc gctgaaccgc gagagattct
     9121 tcactatgca atcaagcata tggcgacttc tcttatcttg gtccacaatc atccttcagg
     9181 agcggtagcg cctagccaaa atgatgatca tgtcactaaa cttgttaaag aagcctgcga
     9241 attgatgggg attgttctct tggaccattt gattgtctct cattctaatt actttagtta
     9301 tcgtgaaaag acagatttaa tctaaagttc attaacgaca tagtcaaaga gttttttatc
     9361 tttgggacga ttttcaaaaa gaagttctgg atgccattgg acaccgagaa aggcgacatc
     9421 atccgtactc atgacagcct caatgatacc atctttagga tcatgagcca caacttttaa
     9481 atttggtgct aagtccttga tgctctggtg gtggaaggag ttgatatgag agatttctcc
     9541 atagatttct tggagaacgg tatctggttc tgttaccaag cgttgagttg tgtactcaac
     9601 agaagaatcc tgccaatggt cttcgatatc ttggtacaaa gttccaccca tggcaacgtt
     9661 aaagagttgg gtaccacggc agacagagaa aatgggcttt ttctgtttaa tagcttcctt
     9721 gatgagggcc agttcgaaga tatctctttg aaggtgatag tcatcactat caatggtttt
     9781 gggttcgcca taaaattttg gatcgacatt ttgcccacct gtcaagatga gcttgtcaat
     9841 caaactgata tagtggcagg ccatttcttg atcaccaatc ggtaggatga tgggaatccc
     9901 tccagcatct ttaacgcctt caacaaagcc ttttgctgcg tagctcatca tgatgtcatc
     9961 atctggatga gttttttcgt ttcctgtaat cccaataact ggttttttca taaaatgatt
    10021 ttcgctttct aatcctcttt tcgcatgaag tagaggaggg tttggagttc acttgtcaaa
    10081 tcgacatact gaacgaccac gtcttttggt aaatgcagat ggactggtga aaaactgaga
    10141 attcctttca caccagcatc aaccaagaga ttagcaacct cttgtgactt gacgctggga
    10201 acagttagga tagcagtctt cacatcagca tccttgattt tatccttgat ctgagaaatc
    10261 ccgtaaatgg gaatcccgtc aggagtttgg gtaccgactt caggatggtc gtctaggtca
    10321 aaggccatga taatcttcat cttgttacgt tcgtggaagc ggtagtggag aagggcatgg
    10381 cccatatttc caataccaac cagcatgaca ttggtaatag agttgtcatt gagcaaatcg
    10441 gcaaaaaatg tcattagttt tttgacatca tagccaaaac cacgacgacc aagttcacca
    10501 aaataggaaa aatcacgacg tacggtcgct gaatcaatac cgatagcctc tgcaatttgc
    10561 ttagagttgg cacgttcaat cttttctgca tgaaatctct taaaaattcg atagtagaga
    10621 gagagtcttt ttgctgtagc ttttggaata gcaaactgtt tatctttcac aaaatcacaa
    10681 cctttctatt cttctatttt atagaaacat tgtgaaaaaa tcaacaaaaa taagaaaaaa
    10741 ctaagaaaaa tcttagtttt gatgtaaaaa atctgcatga gatagaaaac ggtagaggtc
    10801 tccgaccagc ccctgataaa cttttttgcc cctaaaagtc agagaagtca cataaagtgt
    10861 atctggtaag gttacacatc ctgacaaagt caacatgaga gcctcatgat cctcatactt
    10921 gagagtacgc tctacatgat agcagtcctt ataggtcagt tcaaacattt tggctctatc
    10981 tttccgattt tgtaaagaca ccacgttcta ccaagctatc catgaggaag tagaattttt
    11041 cctgatgaat atggtggtct tctgatttga aaatatcaac tagacgaagg ccaaacttgt
    11101 cagtgatatt gattttagcc cctgtaagtt ccttgttaat gatgattttg agttggaagc
    11161 cttcaccgct gtttggcact ttttccaaaa ggcgagtcag ttcatagtta ccaaccttag
    11221 tttcaaaaaa ggtgttatct ttgagggtga attttttaac agaagggcta agagtgtaat
    11281 cgtaacgaca attttttaac tgaatgattt tttcaaatgc catatggcta acctccgata
    11341 atttctttta aggtttttgc gagggtttgt aggtcttcaa cggtattttg tggcgacaaa
    11401 ctgatgcgaa gggattcctt caagcgttct gaatttgcgc catacatggc tttaagaaca
    11461 tggctggatt ggacaacgcc tgcagtacag gctgagccag tagagattga aattccagct
    11521 aaatctagcc gaaggagtaa gaggtcattt ttctgaccag gaaatccaat attgagaaca
    11581 taagggagat gatgttttcc tctattcaag taatactgaa tgccctccag ctctgccaga
    11641 aaggcagttt ctagattttg tacatgttga aaatgttctt cttgtttttc taggtcttct
    11701 tttagggctg caaccatgcc tacgatggca ggcagatttt cagttcctgc acgttttttc
    11761 tgttcctgat ctccgccatg tagataggaa tcaaagtcca tgctagatgc gtagagaaaa
    11821 ccgattccct taggaccatg gaatttgtgg gcagaagcag tgagaaaatc aatgcccaat
    11881 tcttctgaat gaattgggat tttaccaata gcctgaactg catcaacatg ataggcagca
    11941 gggtgttgct tgagtatttg gccaatttca gcgatgggaa gtaggtttcc tgtctcatta
    12001 ttggcaaaca tagtagaaac caaaatcgta tcgtcacgta aagccttttg aatttgctgg
    12061 gctgtgattt cttgattttc tggctggata atggttgctt caaacccaaa gtgttgaacc
    12121 aagtaatcaa ttgtttcaag gacagcatgg tgctcgatgg cagttgtgat gatatgtttt
    12181 ccttgttctt ggtgacgaag acagtagcca atgatggtag tattattgcc ttcagtccca
    12241 ccagaagtga aaaagatatg ttgaggtttt gtccttagta actgggctag ttcctgacgg
    12301 gcttctcgca agagtttgcc agcttgacga ccatgaccat gaatactaga aggatttccg
    12361 tgggtttctt gcataacctt ggtcatagct gaaatagcaa ctgctgacat aggagtcgtt
    12421 gcagcattgt ccaaataaat caaagaatca ccttatttct ttttattgta ggcaaagagt
    12481 gggctgactg gttttctttc gtgaatacgg acgatagcat caccaattaa ctcactagca
    12541 gtgatgtagc atacattttt aggagttttt tcttttgttg ctactgaatc agtcacaaga
    12601 atttctttaa tattagtatt gtcaagaagc tcagcagctc cctcgacgaa gagaccgtgg
    12661 ctagaaacag cataaatttc tgtagctcct tcacgttcaa cgattttaga agcttcagag
    12721 aaggtacgtc ctgtatttaa aatatcatca atcaagatag ctttcttacc ttcaacatca
    12781 ccaataatat aaccttcgtt acgagttgca tcgtcttgag ggtagtcgat aatggcgata
    12841 ggagcatcaa gatattcagc caggctacgc gcacgtttga cacctgaatt tttagggcta
    12901 acgacaacaa catctgaacc aagcaatcct ttatcgcagt aatgttttgc gaatagggga
    12961 acagtgaaaa gattatccac tggaatatca aaaaaacctt gaacctgaac ggcatgtaaa
    13021 tcaagagtca ggatacgatc aactccagcc ttaaccagca tattggcaac tagttttgct
    13081 gtaagtggct cacgaggaca agcaatgcgg tcttgacgtg catagccaaa atatggaagg
    13141 acaacgttga tactgtgggc acttgcacgc acacaagcat cgaccatgat taacagttcc
    13201 attaggtggt tgttgacagg gaaacttgtt gattggatga tgtaaacatc ataaccacgg
    13261 acactttctt cgatatttac ttggatttct ccgtctgaaa attgacgtga tgatagtttt
    13321 ccaagtggga caccaacagc ttgggcaatt ttctgtgcaa tctcttggtt agagttaagt
    13381 gcgaaaagtt tcatgttttt tctatctgac attatagacc gtcctctgta aactttataa
    13441 atcctagtta tatttacctt acatatatga actagggttt gtgtattttt atcttttcta
    13501 ttttaccaaa aaatggagat tatttcagct atttttcata cttttgacaa atcgaaccaa
    13561 ttttgaagga gctttttggt aggaaatctg atttttctct aaaaattgtc ggaaatcctg
    13621 tttgccttgc tcatgatttt ccacttcaag ctccaattcg taatctgtca tatcaaagta
    13681 tcggctctga tccagtgcca tgagaccaat agctgttttc atttcatagc gaagcgttgt
    13741 tagacaacca agaacctgcc agttcttact ttggatacca tgtttcgcca attcatccag
    13801 tactagtcct tgaggaagtt cttccttact cagatagttc tcagcatctt ttagttgcaa
    13861 tttttggttg tattccatgt ttccaacact ctgcgggact ttgagtgtca actcagccca
    13921 gtcttcaaag gttcgaatgc gcatagcgac tttcttttct cgcagttcaa aatcaggcgt
    13981 gtctatgtag taatttgttt gaagaacagg agtgacacct gtgaactggt cttttagacg
    14041 attgtattca tcttttttca atagtgtttt caattcaatt tctaaatgtt tcatttttct
    14101 tacctttttt tatcgttgaa agcggattta tggtataata agcattgtat ttattgtata
    14161 tgaatctgga gaaaaaatca aagatatttt tgacggataa tatgagaaca agggagaata
    14221 tatgacctta gaatgggaag aatttctaga tccttacatt caagctgttg gtgagttaaa
    14281 gattaaactt cgtggtattc gtaagcaata tcgtaagcaa aataagcatt ctccaattga
    14341 gtttgtgacc ggtcgagtca agccaattga gagcatcaaa gaaaaaatgg ctcgtcgtgg
    14401 cattacttat gcgaccttgg aacacgattt gcaggatatt gctggcttac gtgtgatggt
    14461 tcagtttgta gatgacgtca aggaagtagt ggatattttg cacaagcgtc aggatatgcg
    14521 aatcatacag gagcgagatt acattactca tagaaaagca tcaggctatc gttcctatca
    14581 tgtggtagta gaatatacgg ttgataccat caatggagct aagactattt tggcagaaat
    14641 tcaaattcgt actttggcca tgaatttctg ggcaacgata gaacattctc tcaactacaa
    14701 gtaccaaggg gatttcccag atgagattaa gaagcgactg gaaattacag ctagaatcgc
    14761 ccatcagttg gatgaagaaa tgggtgaaat tcgtgatgat atccaagaag cccaggcact
    14821 ttttgatcct ttgagtagaa aattaaatga cggtgtagga aacagtgacg atacagatga
    14881 agaatacagg taaacgaatt gatctgatag ccaatagaaa accgcagagt caaagggttt
    14941 tgtatgaatt gcgagatcgt ttgaagagaa atcagtttat actcaatgat accaatccgg
    15001 atattgtcat ttccattggc ggggatggta tgctcttgtc ggcctttcat aagtacgaaa
    15061 atcagcttga caaggtccgc tttatcggtc ttcatactgg acatttgggc ttctatacag
    15121 attatcgtga ttttgagttg gacaagctag tgactaattt gcagctagat actggggcaa
    15181 gggtttctta ccctgttctg aatgtgaagg tctttcttga aaatggtgaa gttaagattt
    15241 tcagagcact caacgaagcc agcatccgca ggtctgatcg aaccatggtg gcagatattg
    15301 taataaatgg tgttcccttt gaacgttttc gtggagacgg gctaacagtt tcgacaccga
    15361 ctggtagtac tgcctataac aagtctcttg gcggtgctgt tttacaccct accattgaag
    15421 ctttgcaatt aacggaaatt gccagcctta ataatcgtgt ttatcgaaca ctgggctctt
    15481 ccattattgt gcctaagaag gataagattg aacttattcc aacaagaaac gattatcata
    15541 ctatttcggt tgacaatagc gtttattctt tccgtaatat tgagcgtatt gagtatcaaa
    15601 tcgaccatca taagattcac tttgtcgcga ctcctagcca taccagtttc tggaaccgtg
    15661 ttaaggatgc ctttatcggt gaggtggatg aatgaggttt gaatttatcg cagatgaaca
    15721 tgtcaaggtt aagacctttt taaaaaagca cgaggtttct aagggattgc tggctaagat
    15781 taagtttcga ggtggagcta ttctggtcaa taatcaaccg caaaatgcaa cgtatctatt
    15841 ggacgttgga gactacgtta ccatttacat tcccgctgag gaaggctttg aaaccttgga
    15901 ggctattgag catccattag atattctcta tgaggatgac cactttctag tcttgaataa
    15961 accctatgga gtggcttcta ttcctagtgt caatcactct aataccattg ccaattttat
    16021 caagggttac tatgtcaagc aaaattatga aaatcagcag gttcacattg ttaccagact
    16081 agatagggac acttctggct tgatgctctt tgccaagcac ggttatgccc atgcacgatt
    16141 agacaagcag ttgcagaaga aatctatcga gaaacgctac tttgctttgg ttaagggaga
    16201 tggacatttg gagccagaag gggaaattat tgctccgatt gcgcgtgatg aagattccat
    16261 tattaccaga cgagtggcta aaggcggaaa gtatgcccat acttcataca agattgtagc
    16321 ttcttatgga aatattcact tggtctatat tcacctgcac actggtcgaa cccatcaaat
    16381 ccgagtccat ttttctcata tcggttttcc tttgctggga gatgatttgt atggtggtag
    16441 tctggaagat ggtattcaac gtcaggctct gcattgccat tacctatcct tttatcatcc
    16501 atttttagag caagacttgc agttagaaag tcccttgccg gatgatttca gtaaccttat
    16561 tacccagtta tcaactaata ctctataaaa actgtctcag agtataatta ttatcttaaa
    16621 ggagaaaact catggaagtt tttgaaagtc tcaaagccaa ccttgttggt aaaaatgctc
    16681 gtatcgttct ccctgaaggg gaagagcctc gtattcttca agcaacaaaa cgcttagtaa
    16741 aagaaacaga agtgattcct gttttgcttg gaaatcctga aaaaattaaa atttatcttg
    16801 aaattgaagg aatcatggat ggttatgagg tcatcgaccc tcaacattat cctcaatttg
    16861 aagaaatggt ttctgccttg gtggagcgtc gcaagggcaa aatgactgaa gaagatgtac
    16921 gcaaggtttt ggttgaagat gtcaactact ttggtgtgat gttggtttac ttgggcttgg
    16981 ttgatggaat ggtgtcagga gcgattcact caacagcttc aacagttcgt ccagctctac
    17041 aaatcatcaa aactcgtcca aatgtaactc gtacttcagg agccttcctc atggttcgtg
    17101 gtacggaacg ttacctattt ggagactgtg ccattaacat caatccagat gcagaagcct
    17161 tggctgaaat tgccatcaac tcagcaatca cagctaagat gtttggcatc gaacctaaaa
    17221 ttgccatgtt gagctattct actaaaggct cagggtttgg tgaaagcgtt gataaggtcg
    17281 ttgaagcaac taaaattgct cacgacttgc gtcctgacct tgaaatcgat ggtgagttgc
    17341 aatttgatgc agcctttgtt cctgaaactg cagctctgaa agctcctgga agtacggtag
    17401 ctggtcaagc aaatgtcttc atcttcccag gtatcgaggc aggaaatatc ggttacaaga
    17461 tggctgaacg cctgggtggc tttgcggctg taggacctgt tttgcaaggt ttaaacaagc
    17521 cagttaatga tctttctcgt ggatgtaatg cagatgatgt ttacaagttg accctcatca
    17581 cagcagctca agcagttcat caatagtgaa aactataaag cgatatacta tgctatactg
    17641 tagttatgaa actatgtacg aaaagcactg ccattaattc ctgagaacta aattactgat
    17701 tggtgtcaaa aaggaaaact tccaagcgat gatatcctgt ctatacacga cctatagaaa
    17761 tctgtaatat acatgtccgt aaaacgataa attccctttt tgattttaaa tgagtatgaa
    17821 aagagaattt tttcatgaga aaacgcataa tattaagtgt cttgacacct gctattatgc
    17881 gttttctggc tctttgtcaa ctgtagtggg ttgaagaaaa gctaagctcg agaaaggaca
    17941 aattttgtcc tttctttttt gatgttcaga gcgataaaaa tccgtttttt gaagttttca
    18001 aagtttcgaa aaccaaaggc attgtgcttg ataagtttga tgagattatt ggtggcttcc
    18061 agtttggcgt tggaataagg taattgaagg gcgttgacga ttttctcttt atctttgagg
    18121 aaggttttaa acagagtctg aaaaagaggt ggaaaagcaa gagctgatag agattatagt
    18181 ggtgtttcaa gtcttcggaa tagctcaaaa gtttatctag aatttcttta ttagtcaagt
    18241 gcatacgaaa agtagggcga taaaatcgtt tatcactcag tttctgacta tcttgttgaa
    18301 tgagctttca gtatcgcttg atagccttgt attcatggga tttcggatga tggcttgtgt
    18361 tctgctctca agaacagtta tgatattgag cttatcaaag tcctgagcaa taaagctcat
    18421 ctctatctcc cgattgaaac agtcactccc cagactgttt caacgtccta ggacataatc
    18481 tcaggaagac gcgaaaaatc atgctcaaag tgaaaatcat tgatcttgcg aatgacagtt
    18541 gaagttgaaa tagacaactg atgatcaatg tcggtcatag aagtcttttc aattagcttc
    18601 tgatcaatct tttggttgat gatacaagga atttgatgat tcttcttgac gatagaagtc
    18661 tcagcgacca tcattttaga acagtgatag ca
//
LOCUS       DAVKAO010000033        18527 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKAO010000033 DAVKAO010000000
VERSION     DAVKAO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.033"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            30..1166
                     /gene="dnaN"
                     /locus_tag="VWX16_001230"
     CDS             30..1166
                     /gene="dnaN"
                     /locus_tag="VWX16_001230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9158583.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IEAVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1231..1425
                     /locus_tag="VWX16_001231"
     CDS             1231..1425
                     /locus_tag="VWX16_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9158584.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1509..2624
                     /gene="ychF"
                     /locus_tag="VWX16_001232"
     CDS             1509..2624
                     /gene="ychF"
                     /locus_tag="VWX16_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9158585.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2695..3264
                     /locus_tag="VWX16_001233"
     CDS             2695..3264
                     /locus_tag="VWX16_001233"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9158586.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3265..6774
                     /gene="mfd"
                     /locus_tag="VWX16_001234"
     CDS             3265..6774
                     /gene="mfd"
                     /locus_tag="VWX16_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9158587.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTKVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6832..7098
                     /locus_tag="VWX16_001235"
     CDS             6832..7098
                     /locus_tag="VWX16_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9158588.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7091..7459
                     /locus_tag="VWX16_001236"
     CDS             7091..7459
                     /locus_tag="VWX16_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9158589.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7579..8847
                     /locus_tag="VWX16_001237"
     CDS             7579..8847
                     /locus_tag="VWX16_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9158590.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8844..10121
                     /gene="tilS"
                     /locus_tag="VWX16_001238"
     CDS             8844..10121
                     /gene="tilS"
                     /locus_tag="VWX16_001238"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9158591.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10125..10667
                     /gene="hpt"
                     /locus_tag="VWX16_001239"
     CDS             10125..10667
                     /gene="hpt"
                     /locus_tag="VWX16_001239"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9158592.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10683..12641
                     /gene="ftsH"
                     /locus_tag="VWX16_001240"
     CDS             10683..12641
                     /gene="ftsH"
                     /locus_tag="VWX16_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9158593.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            12763..13242
                     /locus_tag="VWX16_001241"
     CDS             12763..13242
                     /locus_tag="VWX16_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9158594.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLADDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGELSHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            13336..13407
                     /locus_tag="VWX16_001242"
     tRNA            13336..13407
                     /locus_tag="VWX16_001242"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13369..13371,aa:Glu,seq:ttc)
     gene            13660..15205
                     /locus_tag="VWX16_001243"
     rRNA            13660..15205
                     /locus_tag="VWX16_001243"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            15257..15329
                     /locus_tag="VWX16_001244"
     tRNA            15257..15329
                     /locus_tag="VWX16_001244"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15290..15292,aa:Ala,seq:tgc)
     gene            15454..18355
                     /locus_tag="VWX16_001245"
     rRNA            15454..18355
                     /locus_tag="VWX16_001245"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            18432..>18527
                     /gene="rrf"
                     /locus_tag="VWX16_001246"
     rRNA            18432..>18527
                     /gene="rrf"
                     /locus_tag="VWX16_001246"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6090 a         3046 c         4248 g         5143 t
ORIGIN      
        1 taatactaaa aataaataaa ggagaatcca tgattcattt ttcaattaat aaaaatttat
       61 ttctacaagc attaaatact actaagagag ctattagttc taaaaatgcc attcctattt
      121 tatcaacagt aaaaattgac gtgaccaatg aaggtattac tttaattggt tcaaatggtc
      181 aaatttcaat tgaaaatttt atttctcaaa aaaatgaaga tgctggtttg ttaattactt
      241 ctttaggttc gatccttctt gaagcttctt tctttatcaa tgtagtatct agtttacctg
      301 atgtaactct tgattttaaa gaaattgaac aaaatcaaat tgttttaacc agtggcaaat
      361 cagaaattac cctaaaagga aaagatagcg aacaatatcc acgaatccaa gaaatttcag
      421 caagcactcc tttaatactt gaaacaaaat tactcaagaa aattattaat gaaacagcct
      481 ttgctgcaag tacacaagag agtcgtccga ttttaacagg tgtccacttc gtattgagtc
      541 aacacaaaga gttaaaaaca gttgcaacag actctcatcg cctaagccag aaaaaattga
      601 ctcttgaaaa aaatagtgat gattttgatg tcgtaattcc tagccgttct ctacgcgaat
      661 tttcagcggt atttacagat gatatcgaag ctgtagagat tttctttgcc aataaccaaa
      721 tcctctttag aagcgaaaat attagcttct atactcgtct cctagaagga aactatcctg
      781 atacagatcg cttgattcca acagacttta acactactat tacttttaat gtggtaaact
      841 tacgccagtc aatggagcgt gcccgtcttt tatcaagtgc gactcaaaat ggtactgtga
      901 aacttgaaat taaggatggg gttgttagcg cccatgttca ctctccagaa gttggtaaag
      961 taaacgaaga aatcgatact gatcaggtta ctggtgaaga tttgaccatt agtttcaacc
     1021 caacttactt gattgattct cttaaagctt taaatagcga aaaggtgacc attagcttta
     1081 tctcagctgt tcgtccattt actcttgtgc cagcagatac tgacgaagac ttcatgcagc
     1141 tcattacacc agttcgtaca aattaagtga aagaggttga gcctggctcg cctcttttat
     1201 gatataatcg aaaaagaaaa ggagagtagt atgtatcaag ttggaaattt tgttgagatg
     1261 aaaaaatcac acgcttgtac aatcaagtcg actggtaaaa aggctaatcg ttgggaaatt
     1321 acacgtgtag gagcagatat caaaataaaa tgcagtaatt gtgagcatgt tgtcatgatg
     1381 gggcgatatg attttgagcg aaaaatgaat aaaattattg actgagaacc cttagttaga
     1441 gggttagcac tttatccctt tttgtgttat aatattaggg attgaaatga aaacggagaa
     1501 tgagaaatat ggctttgaca gcaggtatcg ttggtttgcc aaacgttggt aaatcaacac
     1561 tatttaatgc aattacaaaa gcaggagcag aggcagcaaa ctacccattt gcgactattg
     1621 atccaaatgt tggaatggtg gaagttccag atgaacgcct acaaaaacta actgaaatga
     1681 taactcctaa aaagacagtt cccacaacat ttgaatttac agatattgca gggattgtaa
     1741 aaggagcttc aaaaggagaa gggctaggga ataaattctt ggccaatatt cgtgaagtag
     1801 atgcgattgt tcacgtagtt cgtgcttttg atgatgaaaa tgtaatgcgc gagcaaggac
     1861 gtgaagacgc ctttgtagat ccacttgcag atattgatac aattaatctg gaattaattc
     1921 ttgctgactt agaatcagtg aacaaacgat atgcgcgtgt agaaaagatg gcacgtacgc
     1981 aaaaagataa agaatcagta gcagaattca atgttcttca aaagattaaa ccagtcctag
     2041 aagacgggaa atcagctcgt accattgaat ttacagatga ggaacaaaag gttgtcaaag
     2101 gtcttttcct tttgacgact aaaccagttc tttatgtagc taatgtggac gaggatgtgg
     2161 tttcagaacc tgactctatc gactatgtca aacaaattcg tgaatttgca gcgacagaaa
     2221 atgctgaagt agtcgttatt tctgcgcgtg ctgaggaaga aatttctgaa ttggatgatg
     2281 aagataaaaa agagtttctt gaagccattg gtttgacaga atcaggtgta gataagttga
     2341 cgcgtgcagc ttaccacttg cttggattgg gaacttactt cacagctggt gaaaaagaag
     2401 ttcgcgcttg gactttcaaa cgtggtatga aggctcctca agcagctggt attatccact
     2461 cagactttga aaaaggcttt attcgtgcag taaccatgtc atatgaagat ctagtgaaat
     2521 acggatctga aaaggccgta aaagaagctg gacgcttgcg tgaagaagga aaagaatata
     2581 tcgttcaaga tggcgatatc atggaattcc gctttaatgt ctaaaaatta ataaatggtg
     2641 tcaattaggt tggaaaaaaa ttccaaccct tttggctttt gaaaggaaaa ataaatgacc
     2701 aaattacttg taggcttggg aaatccaggg gataaatatt ttgaaacaaa acacaatgtt
     2761 ggttttatgt tgattgatca actagcgaag aaacagaatg tcacttttac acacgataag
     2821 atatttcaag ctgacctagc atcctttttc ctaaatggag aaaaaattta tctggttaaa
     2881 ccaacgacct ttatgaatga aagtggaaaa gcagttcatg ctttattaac ttactatggt
     2941 ttggatattg acgatttact tatcatttac gatgatcttg acatggaagt tgggaaaatt
     3001 cgtttaagag caaaaggctc agcaggtggt cataatggta tcaagtctat tattcaacat
     3061 ataggaactc aggtctttaa ccgtgttaag attggaattg gaagacctaa aaatggtatg
     3121 tcagttgttc atcatgtttt gagtaagttt gacagggatg attatatcgg tattttacag
     3181 tctgttgaca aagttgacga ttctgtaaac tactatttac aagagaaaaa ttttgagaaa
     3241 acaatgcaga ggtataacgg ataaatggtg accttattag atttattctc agaaaatgat
     3301 cagattaaaa aatggcatca aaatttaaca gataagaaaa gacaactaat acttggttta
     3361 tcaacatcta ctaaggcgct tgcaattgca agcagtttag aaaaagaaga taggattgtg
     3421 ttattgacgt caacttatgg agaagcagaa ggacttgtta gtgatcttat atctatcttg
     3481 ggtgaggaac tcgtctatcc atttttggta gatgatgctc ctatggtgga gtttttgatg
     3541 tcttcacagg aaaaaattat ttcacgggtt gaagccttgc gttttttgac tgattcatct
     3601 aagaaaggga ttttagtttg taatatcgca gcaagtcgat tgattttacc gtctcccaat
     3661 gcattcaaag atagtattgt aaaaatctca gttggtgaag aatatgatca acacgcgttt
     3721 atccatcagt taaaggaaaa tggctatcga aaagttacta aagtacaaac tcagggcgaa
     3781 tttagtcttc gaggagatat tttagatatt tttgaaatat cccagttaga accttgtcga
     3841 attgagtttt ttggtgatga aattgatggt atcaggtcat ttgaagtaga aacacaatta
     3901 tcgaaagaaa ataagacaga actcactatc tttccagcta gtgatatgct tttgagagaa
     3961 aaggattatc aacgaggaca gtcagcttta gaaaaacaaa tttcaaaaac tttatcacct
     4021 attttgaaat catacctaga agaaattctt tcaagttttc accaaaaaca aagtcatgca
     4081 gactctcgga agtttttatc tttgtgctat gataagacat ggactgtctt tgattatatt
     4141 gaaaaagata ctccaatatt ctttgatgat tatcaaaaat tgatgaatca gtatgaagtc
     4201 tttgaaagag acttagcgca gtactttaca gaagaattac agaatagtaa agcattttct
     4261 gatatgcagt atttttctga tattgaacaa atctataaaa aacaaagtcc agtgaccttt
     4321 ttctctaatc ttcaaaaggg tttaggaaat ctcaaatttg acaaaattta tcaattcaat
     4381 caatatccta tgcaggaatt tttcaatcag ttttcttttc taaaagaaga aattgaacga
     4441 tataaaaaaa tggattacac cattattctg cagtctagca attcaatggg aagtaaaaca
     4501 ttggaggata tgttagagga atatcagatt aaattggatt ctagagataa gacaagtatc
     4561 tgtaaagaat ctgtaaactt aatagagggt aatctcagac atggttttca ttttgtagat
     4621 gaaaagattt tattgataac tgaacatgag atttttcaaa agaaattaaa gcgtcgtttt
     4681 cgaagacaac atgtttcaaa tgcagagaga ttaaaagatt acaatgaact tgaaaaaggg
     4741 gactatgttg tccatcatat ccatgggatt ggtcaatatc taggaattga aaccattgaa
     4801 atcaagggaa ttcatcgcga ttatgtcagt gtccaatacc aaaatggtga tcaaatttct
     4861 atccccgtgg aacagattca tctactgtcc aaatatattt caagtgatgg taaagctcca
     4921 aaactcaata aattaaatga cggtcatttt aaaaaggcca agcaaaaggt taagaaccag
     4981 gtagaggata tagctgatga tttaatcaaa ctctactctg aacgtagtca gttgaagggt
     5041 tttgctttct cagctgatga tgatgatcaa gatgcctttg atgatgcttt cccttatgtt
     5101 gaaacggatg atcaacttcg tagtattgag gaaatcaaga gggatatgca ggcttctcag
     5161 ccaatggatc gacttttagt tggggatgtt ggttttggaa agactgaagt tgctatgcgt
     5221 gcagccttta aagcagtcaa tgatcacaaa caggttgtca ttctagttcc gacgacggtt
     5281 ttagcgcaac agcactatac gaattttaag gaacgattcc aaaattttgc agttaatgtt
     5341 gatgtgttga gtcgctttag aagtaaaaaa gagcagactg caacacttga aaaattgaaa
     5401 aacggtcaag tcgatatttt gattggaaca catcgtgttt tgtcaaaaga tgttgtgttt
     5461 gctgatttgg gcttgatgat tattgatgag gaacagcgat ttggtgtcaa gcataaggaa
     5521 actttgaaag aactgaagaa acaagtggat gtcctaacct tgaccgctac gccaatccct
     5581 cgtaccctcc atatgtctat gctgggaatc agagatttgt ctgttattga aactccgccg
     5641 actaatcgct atcctgttca gacctatgtt ttggaaaaga atgatagtgt cattcgtgat
     5701 gctgtcttgc gtgaaatgga gcgtggaggt caagtttact atctttacaa caaagttgat
     5761 acaattgttc agaaggtttc agaattacag gagttgattc cggaggcttc gattggatat
     5821 gttcatggtc gaatgagtga agtccagttg gaaaatactc tattagactt tattgaggga
     5881 caatacgata tcttggtgac gactactatt attgagacag gggtggacat tccaaatgct
     5941 aatactttat ttattgaaaa tgcggaccat atgggcttgt caaccttata tcagttaaga
     6001 ggaagagtcg gtcgtagtaa tcgtattgct tatgcttatc tcatgtatcg tccagaaaaa
     6061 tcaatcagtg aagtctctga aaagagatta gaagcgatta aaggatttac agaattgggc
     6121 tctggcttta agattgcaat gcgagatctt tcgattcgtg gagcaggaaa tcttttagga
     6181 aaatcccagt ctggtttcat tgattctgtt ggttttgaat tgtattcgca gttattagag
     6241 gaagctattg ctaaacgaaa cggtaatgct aacgctaaca caagaaccaa agggaatgct
     6301 gagttgattt tgcaaattga tgcctatctt cctgatactt atatttctga tcaacgacat
     6361 aagattgaaa tttacaagaa aattcgtcaa attgacaacc gtgtcaatta tgaagagtta
     6421 caagaggagt tgatagaccg ttttggagaa tacccagatg tagtagccta tcttttagag
     6481 attggtttgg ttaaatcata cttggacaag gtctttgttc aacgtgtgga aagaaaagat
     6541 aataaaatta caattcaatt tgaaaaagtc actcaacgac tgtttttagc tcaagattat
     6601 tttaaagctt tatccgtaac gaacttaaaa gcaggcatcg ctgagaataa gggattaatg
     6661 gagcttgtat ttgatgtcca aaataagaaa gattatgaaa ttttagaagg tttgctgatt
     6721 tttggagaaa gtttattaga gataaaagag tctaaggaag aaaattccat ttgatatttt
     6781 tcttctataa aatagataaa aatggtacaa taataaattg aggtaataag gatgagatta
     6841 gataaatatt taaaagtatc gcgaattatc aagcgtcgta cagtcgcaaa ggaagtagca
     6901 gataaaggta gaatcaaggt taatggaatc ttggccaaaa gttcaacgga cttgaaagtt
     6961 aatgaccaag ttgaaattcg ctttggcaat aagttgctgc ttgtaaaagt actagagatg
     7021 aaagatagta caaaaaaaga agatgcagca ggaatgtatg aaattatcag tgaaacacgg
     7081 gtagaagaaa atgtctaaaa atattgtaca attgaataat tcttttattc aaaatgaata
     7141 ccaacgtcgt cgctacctga tgaaagaacg acaaaaacgg aatcgtttta tgggaggggt
     7201 attgattttg attatgctat tatttatctt gccaactttt aatttagcgc agagttatca
     7261 gcaattactc caaagacgtc agcaattagc agacttgcaa actcagtatc aaactttgag
     7321 tgatgaaaag gataaggaga cagcatttgc taccaagttg aaagatgaag attatgctgc
     7381 taaatataca cgagcgaagt actattattc taagtcgagg gaaaaagttt atacgattcc
     7441 tgacttgctt caaaggtgat aaaatggaaa atttattaga cgtaatagag caatttttga
     7501 gtttgtcaga tgaaaagctg gaagaattgg ctgataaaaa tcaattattg cgtttacaag
     7561 aagaaaagga aaggaagaat gcgtaaattc ttaattattt tgttgctacc aagttttttg
     7621 accatttcaa aagtcgttag cacagaaaaa gaagtagtct atacttcgaa agaaatttat
     7681 tacctttcac aatctgactt tggtatttat tttagagaaa aattaagttc tcccatggtt
     7741 tatggagagg ttcctgttta tgcgaatgaa gatttagtag tggaatctgg gaaattgact
     7801 cccaaaacaa gttttcaaat aaccgagtgg cgcttaaata aacaaggaat tccagtattt
     7861 aagctatcaa atcatcaatt tatagctgcg gacaaacgat ttttatatga tcaatcagag
     7921 gtaactccaa caataaaaaa agtatggtta gaatctgact ttaaactgta caatagtcct
     7981 tatgatttaa aagaagtgaa atcatcctta tcagcttatt cgcaagtatc aatcgacaag
     8041 accatgtttg tagaaggaag agaatttcta catattgatc aggctggatg ggtagctaaa
     8101 gaatcaactt ctgaagaaga taatcggatg agtaaagttc aagaaatgtt atctgaaaaa
     8161 tatcagaaag attctttctc tatttatgtt aagcaactga ctactggaaa agaagctggt
     8221 atcaatcaag atgaaaagat gtatgcagcc agcgttttga aactctctta tctctattat
     8281 acgcaagaaa aaataaatga gggtctttat cagttagata cgactgtaaa atacgtatct
     8341 gcagtcaatg attttccagg ttcttataaa ccagagggaa gtggtagtct tcctaaaaaa
     8401 gaagataata aagaatattc tttaaaggat ttaattacga aagtatcaaa agaatctgat
     8461 aatgtagctc ataatctatt gggatattac atttcaaacc aatctgatgc cacattcaaa
     8521 tccaagatgt ctgccattat gggagatgat tgggatccaa aagaaaaatt gatttcttct
     8581 aagatggccg ggaagtttat ggaagctatt tataatcaaa atggatttgt gctagagtct
     8641 ttgactaaaa cagattttga tagtcagcga attgccaaag gtgtttctgt taaagtagct
     8701 cataaaattg gagatgcgga tgaatttaag catgatacgg gtgttgtcta tgcagattct
     8761 ccatttattc tttctatttt cactaagaat tctgattatg atacgatttc taagatagcc
     8821 aaggatgttt atgaggttct aaaatgaggg aaccagattt tttaaatcat tttctcaaga
     8881 agggatattt caaaaagcat gctaaggcgg ttctagctct ttctggtgga ttagattcta
     8941 tgtttctatt taaggtattg tctacttatc aaaaagagtt agagattgaa ttgattctgg
     9001 ctcatgtgaa tcataagcag agaattgaat cagattggga agaaaaggaa ttaaggaagt
     9061 tggctgctga agcagagctt cctatttata tcagcaattt ttcaggagaa ttttcagaag
     9121 cgcgtgcacg aaattttcgt tatgattttt ttcaagaggt tatgaaaaag acaggtgcga
     9181 cagctttagt cactgcccac catgctgatg atcaggtgga aacgattttg atgcgcttga
     9241 ttcgaggaac tcgcttgcgc tatctatcag gaattaagga gaagcaagta gtcggagaga
     9301 tagaaatcat tcgtcccttc ttgcattttc agaaaaaaga ctttccatca atttttcact
     9361 ttgaagatac atcaaatcag gagaatcatt attttcgaaa tcgtattcga aactcttact
     9421 taccagaatt ggaaaaagaa aatcctcgat ttagggatgc aatcttaggc attggcaatg
     9481 aaattttaga ttatgatttg gcaatagctg aattatctaa caatattaat gtggaagatt
     9541 tacagcagtt attttcttac tctgagtcta cacaaagagt tttacttcaa acttatctga
     9601 atcgttttcc agatttgaat cttacaaaag ctcagtttgc tgaagttcag cagattttaa
     9661 aatctaaaag ccagtatcgt catccgatta aaaatggcta tgaattgata aaagagtacc
     9721 aacagtttca gatttgtaaa atcagtccgc aggctgatga aaaggaagat gaacttgtgt
     9781 tacactatca aaatcaggta gcttatcaag gatatttatt ttcctttgga cttccattag
     9841 aaggtgaatt aattcaacaa atacctgttt cacgtgaaac atccatacac attcgtcatc
     9901 gaaaaacagg agatgttttg attcaaaatg ggcatagaaa aaaactcaga cgtttattta
     9961 ttgatttgaa aatccctatg gaaaagagaa actctgctct tattattgag caatttggtg
    10021 aaattgtctc aattttggga attgcgacca ataatttgag taaaaaaacg aaaaatgata
    10081 taatgaacac tgtactttat atagaaaaaa tagataggta aaaaatgtta gaaaacgata
    10141 ttaaaaaagt cctcgtttca cacgatgaaa ttacagaagc agctaaaaaa ctaggtgctc
    10201 aattaactaa agactatgca ggaaaaaatc caatcttagt tgggatttta aaaggatcta
    10261 ttccttttat ggctgaattg gtcaaacata ttgatacaca tattgaaatg gacttcatga
    10321 tggtttctag ctaccatggt ggaacagcaa gtagtggtgt tatcaatatt aaacaagatg
    10381 tgactcaaga tatcaaagga agacatgttc tatttgtaga agatatcatt gatacaggtc
    10441 aaactttgaa gaatttgcga gatatgttta aagaaagaga agcagcttct gttaaaattg
    10501 caaccttgtt ggataaacca gaaggacgtg ttgtagaaat tgaggcagac tatacctgct
    10561 ttactatccc aaatgagttt gtagtaggtt atggtttaga ctacaaagaa aattatcgta
    10621 atcttcctta tattggagta ttgaaagagg aagtgtattc aaattagaaa gaataatctt
    10681 taatgaaaaa acaaaataat ggtttaatta aaaatccttt tctatggtta ttatttatct
    10741 ttttccttgt gacaggattc cagtatttct attctgggaa taactcagga ggaagtcagc
    10801 aaatcaacta tactgagttg gtacaagaaa ttaccgatgg taatgtaaaa gaattaactt
    10861 accaaccaaa tggtagtgtt atcgaagttt ctggtgtcta taaaaatcct aaaacaagta
    10921 aagaaggaac aggtattcag tttttcacgc catctgttac taaggtagag aaatttacca
    10981 gcactattct tcctgcagat actaccgtat cagaattgca aaaacttgct actgaccata
    11041 aagcagaagt aactgttaag catgaaagtt caagtggtat atggattaat ctactcgtat
    11101 ccattgtgcc atttggaatt ctattcttct tcctattctc tatgatggga aatatgggag
    11161 gaggcaatgg ccgtaatcca atgagttttg gacgtagtaa ggctaaagca gcaaataaag
    11221 aagatattaa agtaagattt tcagatgttg ctggagctga ggaagaaaaa caagaactag
    11281 ttgaagttgt tgagttctta aaagatccaa aacgattcac aaaacttgga gcccgtattc
    11341 cagcaggtgt tcttttggag ggacctccgg ggacaggtaa aactttgctt gctaaggcag
    11401 tcgctggaga agcaggtgtt ccattcttta gtatctcagg ttctgacttt gtagaaatgt
    11461 ttgtcggagt tggagctagt cgtgttcgct ctctttttga ggatgccaaa aaagcagcac
    11521 cagctatcat ctttatcgat gaaattgatg ctgttggacg tcaacgtgga gtcggtctcg
    11581 gcggaggtaa tgacgaacgt gaacaaacct tgaaccaact tttgattgag atggatggtt
    11641 ttgagggaaa tgaagggatt atcgtcatcg ctgcgacaaa ccgttcagat gtacttgatc
    11701 ctgccctttt gcgtccagga cgttttgata gaaaagtatt ggttggccgt cctgatgtta
    11761 aaggtcgtga agcaatcttg aaagttcacg ctaagaacaa gcctttagca gaagatgttg
    11821 atttgaaatt agtggctcaa caaactccag gctttgttgg tgctgattta gagaatgtct
    11881 tgaatgaagc agctttagtt gctgctcgtc gcaataaatc gataattgat gcttcagata
    11941 ttgatgaagc agaagataga gttattgctg gaccttctaa gaaagataag acagtttcac
    12001 aaaaagaacg agaattggtt gcttaccatg aggcaggaca taccattgtt ggtctagtct
    12061 tgtcgaatgc tcgcgttgtc cataaggtta caattgtacc acgcggccgt gcaggcggat
    12121 acatgattgc acttcctaaa gaggatcaaa tgcttctatc taaagaagat atgaaagagc
    12181 aattggctgg cttaatgggt ggacgtgtag ctgaagaaat tatctttaat gtccaaacta
    12241 caggagcttc aaacgacttt gaacaagcga cacaaatggc gcgtgcaatg gttacagagt
    12301 acggtatgag tgaaaaactt ggcccagtac aatatgaagg aaaccatgct atgtttggtg
    12361 cacagagtcc tcaaaaatca atttcagaac aaacagctta tgaaattgat gaagaggttc
    12421 gttcattatt aaatgaggca cgaaataaag ctgctgaaat tattcagtca aatcgtgaaa
    12481 ctcacaagtt aattgcagaa gcattattga aatacgaaac attggatagt acacaaatta
    12541 aagctcttta cgaaacagga aagatgcctg aagcagtaga agaggaatct catgcactat
    12601 cctatgatga agtaaagtca aaaatgaatg acgaaaaata accctgagag aggctggagc
    12661 ctctcttttt tgtgcagttt aggagctaaa gggaacagaa tggataaaat ggaacaaatg
    12721 tgttttctaa tctgttagac tgtatctaga aaggggaaaa ttatgattaa agaattgtat
    12781 gaagaagtcc aagggactgt gtataagtgt agaaatgaat attaccttca tttatgggaa
    12841 ttgtcggatt gggaccaaga aggcatgctc tgcttacatg aattgattag tagagaagaa
    12901 ggactggcag acgatattcc acgtttaagg aaatatttca aaaccaagtt tcgaaatcga
    12961 attttagact atatccgtaa gcaggaaagt cagaagcgta gatacgataa agaaccctat
    13021 gaagaagtgg gtgagctcag tcatcgtata agtgagggag gtctctggct agatgattat
    13081 tatctctttc atgaaacact aagagattat agaaacaaac aaagtaaaga gaaacaagaa
    13141 gaactagaac gcgtcttaag caatgaacga tttcgagggc gtcaaagagt attaagagac
    13201 ttacgcattg tgtttaagga gtttactatc cgtacccact agtaagtcat gcaaaaaaaa
    13261 tgaaaaaaat tagaaaaagt agttgacaaa gtttgaaaag actgtataat agtaagagtt
    13321 gaaaataaca actcaggtcc gttggtcaag gggttaagac accgcctttt cacggcggta
    13381 acacgggttc gaatcccgta cggactatgg tatgttgcgt caggaccact tgatgaaaaa
    13441 aagtttaaaa aaacttaaaa atcttcaaaa aagtgttgac aagcgaaagc agttgtgata
    13501 tactaatata gttgtcgctt gagagaagca agtgacaaag acctttgaaa actgaacaag
    13561 acgaaccaat gtgcagggcg ctacaacata agttgtagta ctgaacaatg aaaaaaacaa
    13621 taaatctgtc agtgacagaa atgagtaaga actcaaactt ttaatgagag tttgatcctg
    13681 gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag tagaacgctg aaggaggagc
    13741 ttgcttctct ggatgagttg cgaacgggtg agtaacgcgt aggtaacctg cctggtagcg
    13801 ggggataact attggaaacg atagctaata ccgcataaga gtagatgttg catgacattt
    13861 gcttaaaagg tgcacttgca tcactaccag atggacctgc gttgtattag ctagttggtg
    13921 gggtaacggc tcaccaaggc gacgatacat agccgacctg agagggtgat cggccacact
    13981 gggactgaga cacggcccag actcctacgg gaggcagcag tagggaatct tcggcaatgg
    14041 acggaagtct gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaagctct
    14101 gttgtaagag aagaacgagt gtgagagtgg aaagttcaca ctgtgacggt atcttaccag
    14161 aaagggacgg ctaactacgt gccagcagcc gcggtaatac gtaggtcccg agcgttgtcc
    14221 ggatttattg ggcgtaaagc gagcgcaggc ggttagataa gtctgaagtt aaaggctgtg
    14281 gcttaaccat agtaggcttt ggaaactgtt taacttgagt gcaagagggg agagtggaat
    14341 tccatgtgta gcggtgaaat gcgtagatat atggaggaac accggtggcg aaagcggctc
    14401 tctggcttgt aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc
    14461 tggtagtcca cgctgtaaac gatgagtgct aggtgttaga ccctttccgg ggtttagtgc
    14521 cgtagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag
    14581 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag
    14641 aaccttacca ggtcttgaca tccctctgac cgctctagag atagagtttt ccttcgggac
    14701 agaggtgaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc
    14761 ccgcaacgag cgcaacccct attgttagtt gccatcattt agttgggcac tctagcgaga
    14821 ctgccggtaa taaaccggag gaaggtgggg atgacgtcaa atcatcatgc cccttatgac
    14881 ctgggctaca cacgtgctac aatggctggt acaacgagtc gcaagccggt gacggcaagc
    14941 taatctctta aagccagtct cagttcggat tgtaggctgc aactcgccta catgaagtcg
    15001 gaatcgctag taatcgcgga tcagcacgcc gcggtgaata cgttcccggg ccttgtacac
    15061 accgcccgtc acaccacgag agtttgtaac acccgaagtc ggtgaggtaa ccgtaaggag
    15121 ccagccgcct aaggtgggat agatgattgg ggtgaagtcg taacaaggta gccgtatcgg
    15181 aaggtgcggc tggatcacct cctttctaag gataaggaac tgcgcattgg tcttgtttag
    15241 tcttgagagg tcttgtgggg ccttagctca gctgggagag cgcctgcttt gcacgcagga
    15301 ggtcagcggt tcgatcccgc taggctccat tggtgagaga tcaccaagta atgcacattg
    15361 aaaattgaat atctatatca aatagtaaca agaaaataaa ccgaaaacgc tgtagtatta
    15421 ataagagttt atgactgaaa ggtcagaaaa taaggttaag ttaataaggg cgcacggtgg
    15481 atgccttggc actaggagcc gacgaaggac gtgacaaacg acgatatgcc ttgggtagct
    15541 gtaagtaagc gatgatccag ggatttccga atgggggaac ccaacaggta gtacctgtta
    15601 cccacatctg ttaaggatgt gaggaggaag acgcagtgaa ctgaaacatc taagtagctg
    15661 caggaagaga aagcaaaagc gattgcctta gtagcggcga gcgaaacggc agaagggcaa
    15721 accgaagagt ttactcttcg gggttgtagg actgcaatgt ggactcaaag attatagaag
    15781 aatgatttgg gaagatcagc caaagagagt aatagcctcg tatttaaaat agtctttgta
    15841 cctagcagta tcctgagtac ggcgggacac gtgaaatccc gtcggaatct gggaggacca
    15901 tctcccaacc ctaaatactc cctagtgacc gatagtgaac cagtaccgtg agggaaaggt
    15961 gaaaagcacc ccgggagggg agtgaaatag aacctgaaac cgtgtgccta caacaagttc
    16021 gagcccgtta atgggtgaga gcgtgccttt tgtagaatga accggcgagt tacgttatga
    16081 tgcgaggtta agttgaagag acggagccgt agggaaaccg agtctgaata gggcgcctta
    16141 gtatcatgac gtagacccga aaccatgtga cctacccatg agcaggttga aggtgcggta
    16201 agacgcactg gaggaccgaa ccagggcacg ttgaaaagtg cttggatgac ttgtgggtag
    16261 cggagaaatt ccaaacgaac ttggagatag ctggttctct ccgaaatagc tttagggcta
    16321 gcgtcgacat tagagattct tggaggtaga gcactgtttg ggtgaggggt ccatcccgga
    16381 ttaccaatct cagataaact ccgaatgcca atgaattatg gtcggcagtc agactgcgag
    16441 tgctaagatc cgtagtcgaa agggaaacag cccagaccac cagctaaggt cccaaaataa
    16501 ttgttaagtg gaaaaggatg tggggttgca cagacaacta ggatgttagc ttagaagcag
    16561 ctattcattc aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
    16621 ccggggctaa aacaatttac cgaagctgtg gataccttta taggtatggt aggagagcgt
    16681 tctatgtgtg atgaaggtat accgtgagga gtgctggaac gcatagaagt gagaatgccg
    16741 gtatgagtag cgaaagacag gtgagaatcc tgtccaccgt aagactaagg tttccagggg
    16801 aaggctcgtc cgccctgggt tagtcgggac ctaaggagag accgaaaggt gtatccgatg
    16861 gacaacaggt tgatattcct gtactagagt atgtagtgat ggagggacgc agtaggctaa
    16921 ctaaagcaga cgattggaag agtctgtcta agcagtgagg tgtgaattga gtcaaatgct
    16981 taattctata acattgagct gtgatgggga gcgaagttta gtagcgaagt tagtgacgtc
    17041 acactgccaa gaaaagcttc tagcgtttaa acatactcta cccgtaccgc aaaccgacac
    17101 aggtagtcga ggcgagtagc ctcaggtgag cgagagaact ctcgttaagg aactcggcaa
    17161 aatgaccccg taacttcggg agaaggggtg ctgacttaaa gtcagccgca gtgaataggc
    17221 ccaagcaact gtttatcaaa aacacagctc tctgctaaat cgtaagatga tgtatagggg
    17281 gtgacgcctg cccggtgctg gaaggttaag aggagtgctt agcgtaagcg aaggtatgaa
    17341 ttgaagcccc agtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg
    17401 tcgggtaagt tccgacccgc acgaaaggcg taatgatttg ggcactgtct caacgagaga
    17461 ctcggtgaaa ttttagtacc tgtgaagatg caggttaccc gcgacaggac ggaaagaccc
    17521 catggagctt tactgcagtt tgatattgag tgtctgtacc acatgtacag gataggtagg
    17581 agtctaagag atcgggacgc cagtttcgaa ggagacgctg ttgggatact acccttgtgt
    17641 tatggccact ctaacccaga taggtgatcc ctatcggaga cagtgtctga cgggcagttt
    17701 gactggggcg gtcgcctcct aaaaggtaac ggaggcgccc aaaggttccc tcagaatggt
    17761 tggaaatcat tcgcagagtg taaaggtata agggagcttg actgcgagag ctacaactcg
    17821 agcagggacg aaagtcgggc ttagtgatcc ggtggttccg tatggaaggg ccatcgctca
    17881 acggataaaa gctaccctgg ggataacagg cttatctccc ccaagagttc acatcgacgg
    17941 ggaggtttgg cacctcgatg tcggctcgtc gcatcctggg gctgtagtcg gtcccaaggg
    18001 ttgggctgtt cgcccattaa agcggcacgc gagctgggtt cagaacgtcg tgagacagtt
    18061 cggtccctat ccgtcgcggg cgtaggaaat ttgagaggat ctgctcctag tacgagagga
    18121 ccagagtgga cttaccgctg gtgtaccagt tgtcttgcca aaggcatcgc tgggtagcta
    18181 tgtagggaag ggataaacgc tgaaagcatc taagtgtgaa acccacctca agatgagatt
    18241 tcccatgatt atatatcagt aagagccctg agagatgatc aggtagatag gttagaagtg
    18301 gaagtgtggc gacacatgta gcggactaat actaatagct cgaggactta tccaaagtaa
    18361 ctgagaatat gaaagcgaac ggttttcttg aattgaatag atattcaatt ttgagtaggt
    18421 attactcaga gttaagtgac gatagcctag gagatacacc tgtacccatg ccgaacacag
    18481 aagttaagcc ctagaacgcc ggaagtagtt gggggttgcc ccctgtg
//
LOCUS       DAVKAO010000034        18051 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKAO010000034 DAVKAO010000000
VERSION     DAVKAO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.034"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            314..1360
                     /gene="pheS"
                     /locus_tag="VWX16_001247"
     CDS             314..1360
                     /gene="pheS"
                     /locus_tag="VWX16_001247"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9158595.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKIISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1360..1869
                     /locus_tag="VWX16_001248"
     CDS             1360..1869
                     /locus_tag="VWX16_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9158596.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMQEEEIAAFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1946..4351
                     /locus_tag="VWX16_001249"
     CDS             1946..4351
                     /locus_tag="VWX16_001249"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9158597.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVELPAAGLSK
                     IVVGEVLSCEAVPETHLHVCQINVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARDGEKLVTLDGEERDLAETDLVITVADKPVALAGVMGGQATEISEKS
                     SRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVIQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4419..5420)
                     /locus_tag="VWX16_001250"
     CDS             complement(4419..5420)
                     /locus_tag="VWX16_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9158598.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMASILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5568..5971
                     /locus_tag="VWX16_001251"
                     /pseudo
     CDS             5568..5971
                     /locus_tag="VWX16_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6097..6363
                     /locus_tag="VWX16_001252"
     CDS             6097..6363
                     /locus_tag="VWX16_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9158599.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            6624..8873
                     /gene="metE"
                     /locus_tag="VWX16_001253"
     CDS             6624..8873
                     /gene="metE"
                     /locus_tag="VWX16_001253"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9158600.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            8937..9803
                     /gene="metF"
                     /locus_tag="VWX16_001254"
     CDS             8937..9803
                     /gene="metF"
                     /locus_tag="VWX16_001254"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9158601.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10488..12701
                     /gene="pnp"
                     /locus_tag="VWX16_001255"
     CDS             10488..12701
                     /gene="pnp"
                     /locus_tag="VWX16_001255"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9158602.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEITAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12717..13334
                     /gene="cysE"
                     /locus_tag="VWX16_001256"
     CDS             12717..13334
                     /gene="cysE"
                     /locus_tag="VWX16_001256"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9158603.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13346..14230
                     /locus_tag="VWX16_001257"
     CDS             13346..14230
                     /locus_tag="VWX16_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9158604.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERGTASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14312..15655
                     /locus_tag="VWX16_001258"
     CDS             14312..15655
                     /locus_tag="VWX16_001258"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9158605.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15648..16034
                     /locus_tag="VWX16_001259"
     CDS             15648..16034
                     /locus_tag="VWX16_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9158606.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16038..16922
                     /locus_tag="VWX16_001260"
     CDS             16038..16922
                     /locus_tag="VWX16_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9158607.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17281..17631
                     /locus_tag="VWX16_001261"
     CDS             17281..17631
                     /locus_tag="VWX16_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158608.1"
                     /translation="MKLKLNNVDNQPATEVPSSGITVKLNAKDNAGNWTSASNKKEVT
                     VKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHPAGAPTFAKGEGEHAND
                     IVATYSDGTTYYYR"
     gene            17800..17970
                     /locus_tag="VWX16_001262"
                     /pseudo
     CDS             17800..17970
                     /locus_tag="VWX16_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         5438 a         3476 c         4090 g         5047 t
ORIGIN      
        1 gctataaagt tagataagaa taagctaaca agcagaagta agataggtct tgcaagaatg
       61 gtaaaaatct gataaactaa gtagtaattg aataggaatc tgaaagacta gtagcccaag
      121 ggaagtatat cagggaggag agccgtgact gcaagctctc tatatgaaag ctgggtgaat
      181 tcacttgcgc atggatttag aaatgaaggt ctgacttgtc agatgaaaac ggatggtacc
      241 gcgtgtcaac gctccgagtg gagtttttgg catgtggttt tctttttatc tacgagagac
      301 tgatggagga aatatgtcaa ctattgaaga acaattaaaa gcgcttcgcg aagaaacgct
      361 gactagcttg aagcagatta ctgctggaaa tgaaaaagag atgcaagatt tgcgtgtctc
      421 tgtccttggt aaaaagggtt cgcttactga aatcctcaaa gggatgaaag atgtttctgc
      481 tgagatgcgt ccaatcatcg ggaaacacgt caatgaagct cgtgatgtct tgacagctgc
      541 ttttgaagaa acagctaagc tcttggaaga aaagaaagtc gcggctcaac tggctagcga
      601 gagtatcgat gtgacgcttc caggtcgtcc agttgcgact ggtcaccgtc acgttttgac
      661 acaaaccagt gaagaaatcg aagatatctt catcggtatg ggttatcaag tcgtggatgg
      721 ttttgaagtg gagcaagact actataactt tgaacgtatg aaccttccaa aagaccaccc
      781 agctcgtgat atgcaggata ctttctatat cactgaagaa atcttgctcc gtacccacac
      841 gtctccagtt caggcacgtg ctatggatgc ccatgatttt tctaaaggtc ctttgaagat
      901 aatctcgcca gggcgtgtct tccgtcgcga tacggacgat gcgacccaca gtcaccaatt
      961 ccaccaaatc gaaggcttgg tagttgggaa aaatatctct atggctgatc ttcaaggaac
     1021 gcttcagttg attgtccaaa aaatgtttgg tgaagagcgt caaattcgtt tgcgtccatc
     1081 ttacttccca ttcacagagc catctgttga ggtggatgtt tcttgcttca aatgtggtgg
     1141 agaaggctgt aacgtatgta agaaaacagg ttggatcgaa attatggggg ccggtatggt
     1201 tcacccacgt gtccttgaaa tgagtggtat cgatgcgact gtatactctg gctttgcctt
     1261 tggtcttgga caagagcgtg tagctatgct ccgttatgga atcaacgata tccgtggatt
     1321 ctaccaagga gatgtccgct tctcagaaca gtttaaataa tgattagaaa agtagaaatg
     1381 gcagatgttg aggtgttggc taaaattgcc aaacaaacct ttcgtgaaac ctttgcgtat
     1441 gataatacgg aagagcagtt acaggaatac tttgaagagg cttatagtct gaaaactttg
     1501 tcaactgagt tgggaaatcc tgactctgaa acctatttca ttatgcagga ggaggagata
     1561 gctgcttttc tcaaagtcaa ctggggaagt gctcaaactg agagagaatt agaggacgct
     1621 tttgaaattc aacgcctcta tgtgctacaa aaattccaag gatttggact aggtaagcaa
     1681 ctgtttgaat ttgctcttga acttgctaca aaaaatagtt tttcttgggc ttggctaggt
     1741 gtttgggagc ataatacaaa agctcaagcg ttttataacc gatatggttt tgaaaaattt
     1801 agccaacatc attttatggt tggtcaaaaa gtagatacgg attggttact gagaaagaaa
     1861 ttaaggtaag aagaggattc ttctattgaa atgataggaa aggaaaagaa ctagagtgac
     1921 aactgtcatt ctggagaact aaattatgct tgtatcttat aaatggttaa aagaattggt
     1981 ggacattgat gtgccatcac aagagttggc tgaaaaaatg tcaactacag ggatcgaggt
     2041 agagggtgtc gaattaccag ctgctggtct ctcaaaaatt gtcgtcggtg aggtcttgtc
     2101 ttgcgaagct gtgccagaga ctcacctcca tgtttgtcag attaacgttg gcgaagaaga
     2161 agagcgtcag atcgtttgtg gtgccccaaa tgtgcgtgct gggatcaagg tcatggtggc
     2221 tcttccagga gctcgtatcg ctgataacta caaaatcaaa aaaggaaaaa tccgtggttt
     2281 ggagtcactt ggaatgatct gttcacttgg tgaattggga atttctgact cagttgtgcc
     2341 taaggaattc gcagatggca tccaaatctt gcctgaagat gccgtgccag gtgaggaagt
     2401 cttttcttac ctagacttgg atgatgaaat catcgaactt tccatcacac caaaccgtgc
     2461 agatgccctt tctatgtgtg gagtggctca cgaagtggca gccatctatg acaaggcagt
     2521 caactttaaa aaatttactc taacagaaac taatgaagct gcggcagatg ccctttctgt
     2581 cagcattgag acagacaagg cgccttacta tgcagctcgt atcttggaca atgtgactat
     2641 cgcaccaagt ccacaatggt tgcaaaacct tctcatgaac gaaggcatcc gtcccatcaa
     2701 taacgttgta gacgtgacaa actacatcct gctctacttt ggtcaaccta tgcatgcttt
     2761 tgacttggac acatttgaag ggactgacat ccgtgtgcgt gaagcgcgtg atggtgaaaa
     2821 attagtgacc ctggacggtg aagaacgaga cttggctgag acagacctcg tgattacagt
     2881 tgctgacaaa ccagtagccc ttgccggtgt tatgggtggt caggctacag aaatttctga
     2941 aaaatctagt cgtgttatcc ttgaagctgc tgtttttaat ggcaaatcta tccgtaagac
     3001 aagtggtcgc ctgaaccttc gttctgagtc atcttctcgc tttgaaaaag gaattaatgt
     3061 ggcaacagtt aatgaagccc ttgatgcggc agctagcatg attgcagagc ttgcaggcgc
     3121 gacggtgcgt aagggtatcg tttcagcggg tgagcttgat acctctgatg tggaagtttc
     3181 ttcaaccctt gctgatgtta accgtgtcct cggaactgag ctgtcttatg ctgatgtaga
     3241 agacgtcttc cgtcgtcttg gctttggtct ttctggaaat gcagacagct ttacagtcag
     3301 cgtaccacgt cgtcgttggg atatcacaat cgaagctgat ctctttgaag aaatcgctcg
     3361 tatctatgga tatgaccgct tgccaaccag ccttccaaaa gacgatggta cagctggtga
     3421 attgactgtg atacaaaaac tccgccgtca agttcgtacc attgctgaag gagcaggttt
     3481 gacagaaatc atcacctatg ctctgacaac tcctgaaaaa gcagttgagt tcacagctca
     3541 accaagtaac cttactgaac tcatgtggcc aatgactgtg gatcgttcag tcctccgtca
     3601 aaatatgatt tcagggatcc ttgataccgt tgcctacaac gtggctcgta agaataaaaa
     3661 cttggccctt tatgagattg gaaaagtctt tgaacaaaca ggtaatccaa aagaagaact
     3721 tccaaatgag atcaacagct ttgcctttgc cttgacaggc ttggttgctg aaaaagattt
     3781 ccaaacagca gcagttccag ttgatttctt ctatgctaag ggaatccttg aagccctatt
     3841 tactcgtttg ggactccaag taacctatac agcaacatct gaaatcgcta gccttcatcc
     3901 aggtcgtaca gccgtgattt cactcggtga ccaagttctt ggtttccttg gccaagtgca
     3961 tccagtcact gccaaggctt acgatattcc agaaacgtat gtagctgagc ttaacctttc
     4021 agccatcgaa ggggcgctcc aacctgctgt tccatttgtg gaaatcacca aattcccagc
     4081 agtcagccgt gacgttgccc ttctcctcaa ggcagaagtg actcaccaag aagttgtaga
     4141 tgctatccaa gctgccggcg tgaaacgttt gacagatatc aaactctttg acgtcttctc
     4201 aggtgaaaaa ctgggacttg gtatgaagtc aatggcttat agcttgacct tccaaaatcc
     4261 agaagacagc ttaacggacg aagaagtcgc acgctacatg gaaaaaatcc aagcgtctct
     4321 cgaagaaaaa gtcaatgcag aagtgcgtta aacgatagta ggatacaaat tccccctcta
     4381 gtttatgaac actagagggg tgattttata taaaataatt aaaatgtgat ttctgtaaaa
     4441 atacatctat gatcagagtt ttgaaaactt acaatgtcta aatctttaac atagtagtgg
     4501 tttttaggca ataaaatatg atctattgtt gcattaaaaa gttttggact ttggctattc
     4561 caagttcctc tttcaaaagg tggcagtaca tttaatgcgt ccctatgaga gcttattttt
     4621 gcaagtgctc catgacgcat agttgcatta aaatcacctg caataatagc ctttggatat
     4681 tttgaagcca attgattatg aatgatgttt aagtcttgct tccagatttc cattaaacct
     4741 ggcagaggag gagcagtatg caaggcaatg atatctggta tattttgttt tctcgaatgt
     4801 aatacaattg tcccgaaccg tgttgtatga aaagttttag cttctgtata gaaaccataa
     4861 cttttcttga caatcacagt cacaggagct attccactat tggtaggtgg agaagtgaaa
     4921 atatcatagt tggccataga aagtccaact tgatgaaaca atagtttgat tctctggttt
     4981 tcttgctcac ctctgatatt ggtagctagt tcagggaata tagccatatc ggcgtcaaaa
     5041 tggctaaaaa ttcgctcaat atgttgtgct tctatttgat tagcgacgtt ccaagttact
     5101 aattttaagg ttttagtctt atttgatgca gaagaaaggg ttttatctgt tccgaataat
     5161 agtaatatga gcgatagtaa gagcaaaatg ccagagtaaa accgtatttg tttatttttc
     5221 ttgtaaaaag atagggaaaa aaatacgaaa cttaaaatag aagccatagt tgcagtagct
     5281 aaggggaagg cataaatctg tgccaagata ggttgggtag atagccaaat attaagatta
     5341 gaagatggaa aaaaagaata agctatgata gggataacaa agagaatgac cgaaagggca
     5401 gagagaaatg ttttaatcat tgtgtgagta cctcaatttg caatggttgc tcggagctaa
     5461 tgtgacttta cttgattgat attctttttc ttgtcttcct tatagattaa gtacaaccca
     5521 agtaaaaaaa gtccaattta agattgaaat gattaaaagg cacagaaatg caagacaatt
     5581 atactacaaa aggcaaacat ttgacaatcg atagccgtcg cttaatcgaa cgatggaaaa
     5641 aagaaggaaa atcaaataga gaaattgcct ctctacttgg aaaagctcct caaactatcc
     5701 acactgaaat aaagcgtggg acagtccgac aatgtcttgg aaaagggcgc ttcaaagaga
     5761 tttattctgc cgactacgct caacagtctt aagaaactaa aggaaaagat tctccactat
     5821 acataaccaa aaattttcgc ctgaaatgat ggttatggct aaaggggtta acgtgggaat
     5881 ttcaaccatt tactattgga ttcatcgtgg aaaattgggg ttaagcaagc aggatctgct
     5941 ttatcctaga aaaggaaaat cgcttaagaa ataggctagc accaacttta aacccactgg
     6001 tcaatctatc gaatcataat tcgctcattt ttttaaaaat acatagacgt aaaaaaaaga
     6061 gggttaagtg atgaagaata gtgctattgg gagtaattgg aaggatgtcc atctgagctc
     6121 tttagcaagg aggaaatcct tgaaagtgat atgcgagtgg ctatcatgag cgagttgatt
     6181 gaggctaggt atgaacaagg aatcagtcag aaaaagctgg aagaagtcag tggaataagc
     6241 cagcctgtca tagctaggat ggagacagga aagactagtc ctcagttgga tacagtctta
     6301 aaagtcctag ccagtttagg aaagacacta gcaatcgtcc cacttgaaca gggaaaaagt
     6361 tgactagaat cagttatagt cttttctaat aacaggtcat agtcacttat aagaattact
     6421 aataacatgt tttagtattc taattggaat atagctgtaa agtaagaatt acaaaggatt
     6481 tgagacgttt gcttcaagtc tttttctttt gtccgaaaca gatatatagt ttcaaactat
     6541 atcaaaccct aattttttac aattatacag acgctttctt ttctgttaag atagtttcaa
     6601 caacaaattt tggaggacac atcatgtcaa ctacaatcat cggtttccct cgtttgggcg
     6661 aattccgtga attaaaattt acaactgaaa aatactttag aaaagaaatc tcagaagaag
     6721 aacttttagc agcagcaaaa gacttgcgtg ctaaacactg gaacattgtc aaagaaaaag
     6781 gcatcactga aatcccatca aatgactttt ctcactatga taacttccta gatgcggctt
     6841 tccttttcaa cgtagtgcct gcctcagttc aaaacttgga cttgtctgac cttgagcgct
     6901 acttcgcttt gggacgtggt taccagggag aaaaagggga cgttcgcgcc cttccaatga
     6961 agaaatggtt caacactaac taccactaca tcgttcctaa atttgaaaaa gacactcaag
     7021 taaaattggc tggtcacaag attttcgatg agttccaaga agccaaagaa cttggattga
     7081 acactcgtcc tgttcttgta ggtcccttca ctttccttca attgtcagac tttgaagaag
     7141 gcgtgaaagc agacgacttc gtagatagct tagtggctgc ttaccaagaa gtttttgcta
     7201 aattggctga acttggtgcg actcgcatcc aattggatga agcggctctt gtaaaagact
     7261 tgacagctga agaaaaagct ctcttcttga acctttacaa caaactcttg gctgacaaaa
     7321 aaggtcttga agtcttgctt caaacttatt tcggtgatgt tcgtgacgta tacgctgacc
     7381 ttgtaaactt accagtagat gctatcggtc ttgactttgt tgaaggtaag aaaactcttg
     7441 aactcgttaa aggtggcttc ccagctgaca agactctcta tgtaggtatt gtcaatggta
     7501 aaaacatctg gcgcaacaac tacgaaaaga gcttggctgt tcttgagcaa atcccagctg
     7561 aaaacatcgt tttgacaagc tcttgctcac ttcttcatgt gccatttaca actgctaatg
     7621 aagaatttga accagcactc ttgaaccact ttgcctttgc agttgaaaaa ttggatgaaa
     7681 tccgtgactt ggatgctatc cgcaatggtc aaggttcaga agctcttgca gccaacaaag
     7741 aactctttgc gactgagcgt gttggtgaaa atgctgaact tcgtgcgcgt atcgctggct
     7801 tgacagacgc agactacact cgtttgccag cctttgcaga acgtgaagct atccaagaag
     7861 aagctttcaa acttccagct cttccaacaa caacgattgg ttcattccct caaacaaaag
     7921 aagttcgtgc caaacgtttg gcttaccgta aaggtgaatt gtctcaaaaa gagtacgatg
     7981 ctttccttgc tgaaacgatt gacgaatgga tcaagtggca agaagatatt gactttgatg
     8041 tccttgttca cggtgaattt gagcgtaatg acatggttga gtacttcggt caaaacttgt
     8101 caggatacct cttctctaaa aatggatggg tacaatcata cggtatgcgt ggggtaaaac
     8161 caccaatcat ctggggtgat gtcactcgtc ttaaccctat cactgttaaa tggtctagct
     8221 atgcacaaag tcgtacaaac aaacctgtta aaggtatgtt gactggacct gttaccatcc
     8281 ttaactggtc attcccacgt gaagacatct ctatcaagga ttcaactctc caaatcgccc
     8341 ttgctatcaa ggatgaagta cttgaccttg aagctgctgg tgtgaaaatc atccaaatcg
     8401 acgaggctgc tcttcgtgaa aaattgccac tccgccgtag cgactggtac gaagactacc
     8461 ttgactgggc tattcctgcc ttccgcttgg tacactcaac agtagcgcca gacacacaaa
     8521 tccacactca catgtgttac tcagaattta cagatatcat cccagctatc gataacatgg
     8581 atgcagacgt tatttccttt gaagctagcc gttcaaacct tgaaatcttg gacgaactca
     8641 aagcgaaaaa cttccaaaca gaagtgggac ctggggttta cgatatccac tcacctcgtg
     8701 taccaaatga aggcgaaatc gacaacacaa tcgaagccat ccttgctaaa gtgccaagca
     8761 agaaagtttg gatcaaccct gactgtggtt tgaaaacacg tggtatccca gaaacaaaag
     8821 aaagcttgat ccgccttgtt gaagcagcta aagctgcgcg tgagaaattg taagattgga
     8881 tttctctcca tacagtggtt gttcaaaaag aaatcaacta gaaaaggtta ttacatatgt
     8941 cacgccaaac accgtcactc tcatttgaag tgttccctcc aaacccagcc gtgggtaatg
     9001 ataacattat ttcagccttg caggatatgc aggagttggc tccccatttt atcagtgtaa
     9061 ctgccagcaa taataaattt aatatcaagg aaacgacggt tcgtttggct gactttatcc
     9121 aaaatgattt ggcgattccg accattgccc acttgccagc tatttatcta actaaggaca
     9181 aggttgctga aactattgct gacttggaca aagttggggt gcagaaaatc ttggctcttc
     9241 gtggggatat tattccagac gtggaaccac aaaaggattt ccgctatgca accgacttga
     9301 ttgagtttat caaggaacaa acccctcact ttgatattat tggagcttgc tatccagaag
     9361 ggcatccaga ttcgccaaat cagatttcag atattcaaaa tcttaagaag aaagtggatg
     9421 caggctgttc gagcctcgta actcagctct tctttgacaa tgagcgcttc tatgattttc
     9481 aggacaagtg tattttggct gggattgatg ttcccattca tgcaggaatt atgccaattc
     9541 tgaatcgaaa tcaggctctc cgactcttga agacttgtga gaatatccat cttccacgca
     9601 aatttaaagc catcttagac aagtatgagc atgaccctga gtcgctcaga gcagcaggac
     9661 ttgcctatgc agtggaccaa atcgtggact tggtaactca ggatgttgcc ggtgtgcatc
     9721 tctatacgat gaataatgct gatacagcaa aatacatcca tcaagcaacc catgccttgt
     9781 ttaatcacca gtctctagga taataaaagc aaaccattct tctcaggtga ggggaatggt
     9841 tcctttttaa tggcaaagac ctcacttttt aagaaaaata tgataaaata gattctgtac
     9901 gtacttgata caaagatgaa ggtattaaat aatacaatgc atttaaactg taagatttaa
     9961 cgacacgagt gagtcaaatc gatgttattt gacgaaagag ttagtaaagc atttagctaa
    10021 tcgcccgata gcaattagcg tgaaaggttt agatacttta atattaaacc tttctaatga
    10081 tgtatcttga aaactgaact tgcctacaac tctgtggaaa aagataaatc tccctaggag
    10141 cagtcgctcc gtcgttcgat ttcctatctt cctttgagtt gcttgacggc ttggtatctt
    10201 gatgacttga attttggtct catttcttag gaaaaaagat aagcttccta gcactcgtcg
    10261 agtgcgacgt caccttccta tttttctaca gaaatgttcg gcaagccgaa ccgtccaaaa
    10321 tatcttgtgt aattgaacac ggccgaaaag ctgtgtaaaa aagataaact gtcttgtctt
    10381 catcgaagac ttcgtcagtt tcctattttt actttgcttt tgacgtcctt ggtatcttga
    10441 tctttgtagg caaggcgtat aatttcatca atccaaaggg gattaaaatg gcaaaacaag
    10501 tgtttcaaac gacttttgcg ggtcgtgagt taattgtaga gactggtcag gttgctaagc
    10561 aagcaaatgg atctgttgtc gtacgttacg gtgagtcaac tgtcttgact gctgccgtta
    10621 tgtctaagaa aatggcaact ggggatttct tcccactcca agtcaactac gaagaaaaaa
    10681 tgtatgcggc tgggaagttt cctggtggct ttatgaaacg tgaaggacgt ccttcaacag
    10741 atgcgacctt gacagcgcgt ttgattgacc gtccgattcg tcctatgttt gcggaaggtt
    10801 tccgtaatga agtccaagtc atcaatacag tgctttctta tgatgaaaat gcatctgcac
    10861 caatggctgc tatgtttggt tcatctttgg cactgtctat ttcagatatt ccatttgacg
    10921 gaccaattgc tggggtacaa gtgggatatg tagatggcca aatcatcatc aacccaagtc
    10981 aagaacaagc agagcaatct cttcttgaat tgacagtagc tggaaccaag cacgctatca
    11041 acatggtaga gtctggtgcc aaagaattgt cagaagaaat catgttggaa gcgcttctta
    11101 aagggcacga agctgtcaaa gaattgattg ccttccaaga agaaatcgtt gctgctgtcg
    11161 gtaaagaaaa agcagaagtg gaattgcttc acgtggatgc tgaattgcaa gctgaaatca
    11221 ctgcagccta caacagtgac ctccaaaagg cagttcaagt agaagagaaa ttggcccgtg
    11281 aagctgcaac tcaagcagtg aaagaccaag tgactgccgt ttacgaagaa aaatatgcgg
    11341 accacgaaga atttgaccgt attatgcgtg atgtggctga aatcttggaa caaatggaac
    11401 acgcagaagt gcgacgttta attacagaag acaaggtgcg tcctgatggt cgtaaggtcg
    11461 atgaaatccg tcctttggat gcggttgttg acttccttcc tcgtgtacat ggttcaggtc
    11521 tctttactcg tgggcaaact caagctcttt cagtcttgac cttggctccg atgggagaaa
    11581 ctcaaatcat tgatggtttg gatccagagt acaagaaacg ctttatgcac cactataact
    11641 tccctcaata ttctgtaggg gaaacaggtc gttacggtgc gccaggtcgt cgtgaaatcg
    11701 gtcacggtgc ccttggtgag cgtgctcttg ctcaagtctt gccaagcttg gaagaattcc
    11761 catacgctat ccgtctagta gcagaagttt tggaatcaaa cggttcttca tctcaagctt
    11821 ctatctgtgc gggaactctt gcccttatgg ctggtggtgt gccaatcaag gcgccagtag
    11881 ctggtattgc tatgggactt atctcagatg gaaataacta cacagtattg acagatatcc
    11941 aaggtttgga agatcacttt ggagatatgg acttcaaggt tgcaggtact cgtgatggga
    12001 ttacagccct tcaaatggat atcaagattc aagggattac tgcagaaatc ttgacggagg
    12061 ctcttgctca agccaagaaa gcgcgttttg aaatccttga tgtcattgaa gcaaccattc
    12121 cagaagttcg tccagaattg gctccaactg ctccgaaaat tgatacgatc aagattgatg
    12181 tggacaagat taagattgtc atcggtaagg gtggagaaac catcgacaag attatcgctg
    12241 aaacaggtgt taagattgat atagacgaag aaggaaatgt gtctatctac tctagtgacc
    12301 aagatgctat taaccgtgcc aaagaaatta ttgctggttt ggttcgtgaa gccaaagtgg
    12361 atgaagttta ccgtgctaaa gtcgttcgta tcgagaaatt tggtgccttt gttaacctct
    12421 ttgataagac agatgccctt gttcatatct ctgagatggc ttggactcgt accaatcgtg
    12481 tagaggattt ggtagaaatc ggggatgaag ttgatgttaa ggttatcaag attgatgaaa
    12541 aaggccgtat cgatgcctct atgaaggctc ttctacctcg tccgccaaaa cctgagcatg
    12601 atgaaaaagg tgaaaagtct gagcgccctc accgcccacg tcatcacaag gatcacaaac
    12661 ctaagaaaga atttacagaa acaccaaaag attcagagta agaaaaggag aaatgtatgg
    12721 ggtggtggcg cgaaaccatt gatatcgtaa aagaaaatga tccagcggcc cgcaccactt
    12781 tggaggtttt gctgacttat ccaggtgtca aggccttggc ggcccaccgt ctctcgcatt
    12841 ttctctggaa gtacgacttc aaactcttag ctcgtatgca cagtcagttt tggcgctttt
    12901 ggactcagat tgagattcat ccaggagccc agattgattc aggtgtcttt attgaccatg
    12961 gttctggtct ggtgattgga gagacagcga ttgttgaaaa aggcgttctt ctctatcacg
    13021 gagtgactct cgggggaaca gggaaagact gtggcaaacg ccatccgact gtacgaaagg
    13081 gagccctcat atcagcccat gcccaagtta tcgggcctgt ggaaattggt gaaaatgcca
    13141 aggtcggtgc tgcagcagtt gtcgtagcag atgtacctag tgatgtgacg gttgtcggta
    13201 ttccggccaa gattgtccgt cttcatggta agaaagatga gcctgttatt cacgaagtcg
    13261 aagaaaaacg ggagtattat gtcaataaac tcgagcaggc taaagatgcc agtcacagat
    13321 cgtctggttt gtagaggata cctatatgat tcaagcaaga aacaagttaa gccaagaaga
    13381 gctatctgag gcgaaaaaag taattaactg ttgccaaaac tatgacggta cttatcgcga
    13441 tccctatctc tctaacatgc ttaattttga tccaaacatg cccgcctttt tcctttatta
    13501 tgaaaaaggc gaacttgttg gtttattaac tgtctatgca gatgaccaag atgtagaagt
    13561 gacgatactg gttcatccag gtcatcgccg tcaggggatt gcgcgtgcat tgtttactag
    13621 ttttgagaga ggaacagctt ctttccccat tcggtcagtc acttttcaga cagaacgtat
    13681 ttttttagag aatcatcctg actttgccag taactgggga ctaatagagg atgaagagac
    13741 agaaacttgg ttaggtaagg atagaagacc ttatccgtta gcaaatgttt cccatcttga
    13801 agttttgtta gcagatagtt cgtatcagga tcaaattagt caattaaaat ttcaggcatt
    13861 ttcagaggaa catgaatcga gagaagttgt ggatagatat gtcgctgaag ctctgaagga
    13921 tccagaaagt cgcttatata ttttattaaa agacggtcag gttattggga cttgtacggt
    13981 tgatttatcg actaatacga attacttcta cggtttagca atattggaac ctgaacgtgg
    14041 aaaaggctat ggaagctact tagcaaaatc cctcgtcaac caactaattg agcaaaatga
    14101 caaggaattt cagattgcag tggaagatag caatgtaggt gccaaacgtt tgtatgaaaa
    14161 aattggcttt gtcaaacaga ctcaggtggt ttatctgaat gagaaaggag caagggattc
    14221 cgaagtgtag agatattcgg actgaaattt acttgaactt ttagtgatga aactaattgt
    14281 tcttggattt cagctttcct gattatgatt tatgattaaa atctatgaca ccatgtctcg
    14341 tgatttgcga gaatttgtcc cgattgagga cggcaagatc aagatgtatg tttgtgggcc
    14401 aacggtgtac aactatatcc acgtgggaaa cgcccgttcg acggtagctt ttgatacgat
    14461 tcgtcgctat tttgagtacc gtgggtataa ggttgcctat atttccaatt ttacagatgt
    14521 ggatgataag attatcaacc gtgccaggga agaaggcatc acgcctcagg aggttgcgga
    14581 taagtacatc gctgcctttc gtgaggatgt gacggccttg ggcgtgaaac ctgcgactcg
    14641 ccatccgcgt gtagtggagt ttatggcaga catcatccgt tttgtggaag acttgatcga
    14701 gaaaggcttt gcctatgaga gtcaagggga tgtctatttc cgtgtagaaa aatcccacaa
    14761 ctatgctaaa ttggctaata aaaccttgga agatttggag ctaggtgctt caggtcgtac
    14821 cgatgaagaa acggctcgta aggaaaatcc tgtagacttt gctctatgga aatcttccaa
    14881 accaggtgag atttcttggg atagtccttg gggacctgga cgtccgggtt ggcatattga
    14941 gtgttcagtc atgtcgacag agattttagg tgataccatt gatatccacg gtggtggagc
    15001 tgacctagag tttccacacc acaccaatga aattgcccaa tctgaagcta aaacaggcaa
    15061 ggcttttgct aactactgga tgcacaatgg ctttgtcaat atcgacaatg tcaaaatgtc
    15121 taagtccttg ggtaacttca ttacggtaca cgatgccctt aaaactcttg atgggcaagt
    15181 acttcgtttc ttctttgcga cccagcacta ccgtaagcct atcaacttca cggaaaaggc
    15241 agttcgcgat gccgagacca atctcaagta tctgaagaat acttacgaac aaccatttac
    15301 aggaaacgtg gatgcccaag agttacaaaa ctttaaagat aagtttgtag cagctatgga
    15361 tgaagatttt aatgctgcca acggtatcac agttgtcttt gaaatggcca aatggatcaa
    15421 ctcagggaac tatgatgcaa gtgtcaagca agctcttgca gatatgttag aaatttttgg
    15481 aattgtcttt gttgaggaag ttttggatgc agagattgaa gacttgattc aaaaacgcca
    15541 agaggcgcgt gccaatcgag actttgagac agcagaccaa atccgtgacc aattggttac
    15601 tcaaggaatt aagctccttg ataccaagga tggagtgagg tggacacgtg attgatgtca
    15661 atctcattaa cgggattgcg ctagcctttg agggggatgc ggtgtattct atgtatattc
    15721 gccgtcacct catcctcaaa ggtatgacca aacccaataa actccatcaa gaagcaacca
    15781 agtatgtgtc agccaaggct caggctcgcc tgattgctct catgttggag gagcaggtcc
    15841 taacggaaaa agaagaagaa atctacaaac gtggccgcaa taccaatagc cacacaaagg
    15901 ctaaaaatgc agatgtcgtg acttatcgta tgtccacggg atttgaagcg gttatgggct
    15961 atctccatat gactgagaat ctggaacgtc ttgagagttt ggtttcatgg tgcatccaaa
    16021 aagtggaggg ctagaacatg agggaaaaag aactacaaga ctggtttcct gaggctcgga
    16081 tttcagacca accagtagag aaagagggct atctcacgct ccctttagct tctcagcagt
    16141 ggattttgct ggaggaagct gggctcagcg agcgtgaaaa gcagttggtt gcccttttga
    16201 cccagcagga gcaggctcgt tcgctaaacc cttggtattc ctatctggtt gaaggcaagg
    16261 gacaggcacc gcaagttttt aaaaagattc agttggttta ttgccatctt tcttattttc
    16321 agcaggaaaa tctggcttct tggctagata tgatgcggac tctttttccg aattgtcaga
    16381 cagtgctaca ggtcggatct caggattatg ttttcgtgct tcaacaagac aaatacactt
    16441 ctgtaagaga tattttaagt gatacgattg aagcggttga gtatgacttt ggacttcgtc
    16501 tttctatcat gttgggtcag gtttggtctc agacgggaca tcaagcccta tcagacttaa
    16561 tcaaagctga gcgggatttg ttcaagacat ggtggcgtca gggtcaccaa ggtgttcata
    16621 ctttttctca gctctatctt tggagtatgg gagaaagact cgtggacttg aagccaatca
    16681 aggaatgtct acaccagatg attttggatc aagatcagat tcaggaaatc attctctctc
    16741 tttgggaaaa tagtgctgtt ctcactaaaa cagcccagca actctatctg caccgcaatt
    16801 ctctccaata caagattgat aaatgggaag agttgacagg gcttcagttg aaagagttga
    16861 ccgacctgac cttgtgttat caattgattt taggttcttt gtcaactata gttggtttgt
    16921 aaagaagtta atatttggag aagaggatgg ccatcttctc catttttatg tgcagagtta
    16981 tagtggcttg atgctgggaa agtacactgt gactgctaaa acattcctag aagttgcttt
    17041 gatttcccta atctatttat gtgaatttta tgttatttta ctataacagt tgttgctaag
    17101 ctaaataata gtggggaagt ttatttagac gtaagcattg aagataatca gggaggtagt
    17161 gggtataatt tcagttctgt ttcaagtagc tcacaaacag ctaaatatga aggaactggt
    17221 tataataaca attcatcatt atatataacg attgataaaa cgtctgatgc aacagctctt
    17281 ttgaaattaa agttgaataa tgttgataat caacctgcta ctgaagttcc tagttcagga
    17341 attactgtaa aattaaatgc taaagataat gctggaaact ggacaagtgc ttcgaataaa
    17401 aaagaagtaa cagtaaaaat tgtttctgct aaaccgacat atccagacaa aattttagtg
    17461 aaaaatcctg ataatataaa agatacagaa aaaaatgcca ttattgaaaa attgaaagag
    17521 gcaaataaaa atcatccagc aggagctcca acctttgcta aaggtgaagg agagcatgca
    17581 aatgatattg tagcaactta ttcagatggt acaacttatt attaccgtta aatgatgtga
    17641 caaaatatgc gaggtagtgg ctgtaccact cacttattca cctccccgtg atttgtagta
    17701 gtgataggct ttctcactat tattataaaa caaaataaag atcacaacac tttttcattc
    17761 tgtgttgtgc cttgagtgaa acgaaaggaa tgaattataa atatgaaaag tatagtcact
    17821 aacatagatg agcgcttgcg tactcgccta cgagtgatta tctggaagca atggaagaag
    17881 aaatcgagac gattatgggg attgcttaag ttaggggttc ctaaatggat agcagataag
    17941 gtatctggct ggggcgacca ctatcaatta tattaaaatg aaataagaat aggacgaatc
    18001 gatcagggaa gtcaaatcga tttctaacaa tattttagaa gtagaggtat a
//
LOCUS       DAVKAO010000035        17718 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKAO010000035 DAVKAO010000000
VERSION     DAVKAO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.035"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..114
                     /locus_tag="VWX16_001263"
     CDS             <1..114
                     /locus_tag="VWX16_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9158609.1"
                     /translation="DSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQAKER"
     gene            complement(166..753)
                     /locus_tag="VWX16_001264"
     CDS             complement(166..753)
                     /locus_tag="VWX16_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9158610.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            995..1666
                     /gene="sdaAB"
                     /locus_tag="VWX16_001265"
     CDS             995..1666
                     /gene="sdaAB"
                     /locus_tag="VWX16_001265"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9158611.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            1675..2547
                     /gene="sdaAA"
                     /locus_tag="VWX16_001266"
     CDS             1675..2547
                     /gene="sdaAA"
                     /locus_tag="VWX16_001266"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9158612.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            2569..3201
                     /locus_tag="VWX16_001267"
     CDS             2569..3201
                     /locus_tag="VWX16_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9158613.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKTKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            3251..3526
                     /locus_tag="VWX16_001268"
     CDS             3251..3526
                     /locus_tag="VWX16_001268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158614.1"
                     /translation="MKIKEQTRKLAAGCSKHCFKVVNGTDEVSSKHCFEVVDGTDEVS
                     SKHCFEVVDRTDEVSNHIYGKATLTWFEEIFEEYKSLHNKTHITKVV"
     gene            complement(3551..5401)
                     /locus_tag="VWX16_001269"
     CDS             complement(3551..5401)
                     /locus_tag="VWX16_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9158615.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(5443..6600)
                     /locus_tag="VWX16_001270"
     CDS             complement(5443..6600)
                     /locus_tag="VWX16_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9158616.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIIGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            6658..7824
                     /locus_tag="VWX16_001271"
     CDS             6658..7824
                     /locus_tag="VWX16_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9158617.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTFFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHHYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            7963..8289
                     /locus_tag="VWX16_001272"
     CDS             7963..8289
                     /locus_tag="VWX16_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9158618.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            8276..8869
                     /locus_tag="VWX16_001273"
     CDS             8276..8869
                     /locus_tag="VWX16_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9158619.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            8862..9773
                     /locus_tag="VWX16_001274"
     CDS             8862..9773
                     /locus_tag="VWX16_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9158620.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPIY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            9836..10900
                     /locus_tag="VWX16_001275"
     CDS             9836..10900
                     /locus_tag="VWX16_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158621.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTKIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPYQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKFETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(11077..11937)
                     /locus_tag="VWX16_001276"
     CDS             complement(11077..11937)
                     /locus_tag="VWX16_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9158622.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDVPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEVDGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPLSFYHQLDANQIEVQPILD
                     KDFKALAQEE"
     gene            complement(12212..13198)
                     /locus_tag="VWX16_001277"
     CDS             complement(12212..13198)
                     /locus_tag="VWX16_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9158623.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(13470..14945)
                     /locus_tag="VWX16_001278"
     CDS             complement(13470..14945)
                     /locus_tag="VWX16_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9158624.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(15029..15952)
                     /locus_tag="VWX16_001279"
     CDS             complement(15029..15952)
                     /locus_tag="VWX16_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9158625.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(15966..16895)
                     /locus_tag="VWX16_001280"
     CDS             complement(15966..16895)
                     /locus_tag="VWX16_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9158626.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLSILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(17214..17435)
                     /locus_tag="VWX16_001281"
     CDS             complement(17214..17435)
                     /locus_tag="VWX16_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158627.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(17389..17649)
                     /locus_tag="VWX16_001282"
     CDS             complement(17389..17649)
                     /locus_tag="VWX16_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158628.1"
                     /translation="MTGVSNPTTDSAPLVLAEAKKSFADDSLIEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         5216 a         3830 c         3329 g         5343 t
ORIGIN      
        1 gattctaaac ttatccaaaa agggttctcc cttctggagc taaccgagga aacttctatg
       61 ctgtctcatc tcaaaaaaga agtagagatc tattaccaag cgaaggaaag ataaaaaagc
      121 cgagggattt cctcgactct ttacatatta tttgttcgtt agttcttaat accagccatt
      181 gttaagccag aagtttttag cagcagtcca tgaaccgtaa cgtcctgcaa cgtaggcatc
      241 tgctacacgt tcttggtttt cagctgagta gtcaccgttc aggtatgaat ctgttaattg
      301 gtaacgtccg atataacgtc catttgtagc tgtatagcta ccacctgatt ctttttgagc
      361 gatccattct ttggcttctg cttcagatcc gcttacagtt gaaacaactg tttcacttac
      421 tactggagtt tctgctactg gagctgaaac agtttcatct tgagcggctg gtgccgcata
      481 agtagctggc gctggtgttg caacaggcgc tacagggcca tcgataacca actcttgatc
      541 aacataaatc aaatgaatgt tatcaatgtg gttgttttct gccaattttt caactgttgt
      601 gttgtgagtt tcagcgattt ctgaaagtgt atcaccttct ttaacagtgt aagttgatga
      661 ttcttgagca gatacaaatg atggagcaaa tactgcaaac aaggcagcta ctcctgcaag
      721 agttgcttta atctttttag ttattgattt catattcgaa aattctcctt ctttctatag
      781 ttctatcata ccctttaaat attaccgttt cttgacactt ttatgtagaa atattacaaa
      841 attatttttt atttccctaa ataaggtgtt ttttgtcata aaagatacta tttttatgct
      901 atttgaggta ttagaaaggt tttcatccac aattaaagcc aagcccctca ttctttgata
      961 taatagagat aagaattttt ataaggggaa actgatgaaa tcacttcgtt ttcaatctgt
     1021 ctttgatatc atcggaccag ttatgattgg cccatctagt agccataccg ctggtgctgt
     1081 tcgtattggg aagattgtct cttccatttt tgatgatact ccgacagaag ttgaattcca
     1141 actttttaac tcatttgcca agacctatcg tggtcacgga acagacctag cccttgttgc
     1201 aggtattttg ggaatggata cagatgatcc tgaaattcca aatagtctgg aaattgccca
     1261 caagcgtggt atcaagattg tctggaccat tcagaaagac agcaatgccc ctcaccctaa
     1321 caccactaaa attactgtta aaaatgccca caagaccatc agcgtgactg gtatttctat
     1381 cggtggagga aatattcagg taaccgaact caatggtttt gccgtctctc tcaatatgaa
     1441 tacaccgact atcatcatcg ttcatcaaga tattccaggt atgattgccc ttgttacaga
     1501 ggcgctttcc cgctatggta tcaatatcgc ccagatgaat gtcactcgtg aaaaagctgg
     1561 tgaaaaagcc attatgatta tcgaagttga cagtcgcaac tgtgatgagg ctatcgaaga
     1621 aattcgaaaa atccctcatc tccacaatgt caatttcttt aaataggagg aagcatgttt
     1681 tattctatca aagaattggt cgagcaagca gatctggact ttcaaggaaa tgtcgcagaa
     1741 ctcatgatta caacagagtt tgaattgacc ggtcgcgaac gtgaagaagt cttccttctc
     1801 atggaacgca atctggaagt tatgaaagcc tctgtccaac ttggcctcaa tgaaaataaa
     1861 tctcgtagtg gcctgacagg tggagatgct gccaaattgg atcactacat cgaaaacgga
     1921 aaaactctgt cagattatac gattctctct gctgcccgaa atgctatcgc agtcaatgaa
     1981 cacaatgcta aaatgggctt ggtctgcgct actcctaccg caggaagtgc cggttgtctc
     2041 ccttccgttc tcactgctgc tattgaaaaa ttagacctca gccacgagca acaattggat
     2101 ttcctctttg ctgctggtgc ctttggacta gtcatcgcaa acaatgcctc catctcaggt
     2161 gctgagggtg ggtgtcaagc tgaagttggt tcagcctctg ctatgagtgc tgccgccttg
     2221 actctggctg caggtggaac accttatcag gccagtcaag ctattgcctt tgtcattaaa
     2281 aatatgctag gcctcatctg tgaccctgtt gcaggtttgg tcgaagttcc ctgtgtcaaa
     2341 cgtaatgcca tgggagctag ctttgctttc atcgcagcag acatggcctt ggcaggtatc
     2401 gaatctaaaa tccctgtgga tgaagtgatc gatgccatgt accaagtagg agcaagcatg
     2461 ccaactgcct ttcgtgaaac agctgaaggt ggactcgcta ccacccctac tggtcgtcgc
     2521 ctccaaaaag aaattttcgg agaataagct tatcaaatag gagaaaccat gacctctatc
     2581 acagcgattt ttttcgattt ggatggaacc ctcgttgata gttctatcgg gattcacaat
     2641 gcctttacct atacctttaa agagctgggg gtgcctagcc ctgatgccaa aactattcgt
     2701 ggttttatgg gaccacctct cgaaagtagt tttgcgacct gcctgtccaa agaccaaatt
     2761 tctgaagccg tgcagatata tcgttcttac tataagacaa aaggcatcta tgaagctcaa
     2821 ctctttcctc agattataga cttgcttgag gagttatcga gcagttatcc actctacatc
     2881 accacgacaa agaatacatc tactgctcaa gacatggcaa aaaacttgga aatccatcat
     2941 ttctttgatg gcatttatgg ttctagccct gaagcacccc ataaggcaga tgtcattcac
     3001 caagccttgc agacacatca actagcacca gaacaagcca tcatcatcgg agataccaag
     3061 tttgatatgc tgggagctcg agaaacaggg attcagaaat tggccatcac ttggggattt
     3121 ggagagcaag cagatctact aaactatcaa cctgattata tcgctcacaa acccttagag
     3181 gttttggcgt attttcaata gcatagactg ggtaacaatt ccatttcaga agaaaatgaa
     3241 gtaatactca atgaaaatca aagagcaaac taggaaacta gctgcgggct gctcaaaaca
     3301 ctgttttaag gttgtaaatg gaactgacga agtcagctct aaacactgtt ttgaggttgt
     3361 ggatggaact gacgaagtca gctctaaaca ctgttttgag gttgtggata gaactgacga
     3421 agtcagtaac catatctacg gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga
     3481 gtataagtct ctacataata aaacgcatat taccaaggtt gtttaacttg atactatgcg
     3541 tttttctttt ttattcaatg tgggttgttt gattagcaaa ggcgataata gcttgcttca
     3601 actcatctcc actgagagtg cggaactctc caatcttttg attgatggat tctaaaggca
     3661 tgacattaac cttaccaacg aaaatcttat ccataacttg attatcaaca agttcggttg
     3721 ataccaagag ttcttcgtag agtttttcac ctgggcggat tccaacttca acgattggaa
     3781 tttcactttc agtgtggcca cttagaagca ccatcttctt ggccaagtca taaatcttga
     3841 ctggtttgcc catatcaagg ataaagactt ccccatcttt ggcataagca ccagcatgga
     3901 taaccagacg gctagcttct ggaatggtca taaagtaacg ggtcatgcgg aagtctgtca
     3961 ccgttacagg cccaccttca gcaatctgac gttcaaagac tggaatgaca ctaccacggc
     4021 taccaagaac attcccaaaa cgaactgcac agtaggttga ttggctacgt tggttaaagc
     4081 cagtgacaat caactccgcc acgcgcttgg ttgctcccat aacatttggt ggattaactg
     4141 ccttatctgt cgaaatcata accatcttag acactttagc ttcatcaaca gccttagcaa
     4201 cattgtaagt tccacggata ttgtttttga aggcttcttt tggattgcgc tccatcatag
     4261 gaacatgctt gtgggctgcc gcatgataaa caatagcagg tttgtactgc tcaaagactt
     4321 gcaacaaacg atcatagtct tgaatgtccg caatcacagg tacataatca atcccttgga
     4381 acttacgaat caattcatga taaacaaggt agattgagtt ttccccatga ccgagcaaga
     4441 caatgcgttc aggattgaag cgactaactt gacgacagat ttcagaaccg attgaacctc
     4501 cagctcctgt gactaagatg gtcttacctg tcagttctgc acccagacgc gattcgtcaa
     4561 gacggatttc ctgacgaccc aaaaggtccg taatatcaat tttttggaag ccagtacctg
     4621 cttggtgaag gccctgaaca acagtttcaa ccttaggcat cttgtaacat ttgacaccca
     4681 gcttattaca catctgcaag atacgctcat attctgacgg atccagcgac ggaatcgcaa
     4741 cgatgacacg ctcgatttga tggcgtttgg ctaattcagg cagattgtca taagagccca
     4801 aaacaggaat accaccaagt ttttgaccct ttttcttaga atccttatcc aaaataccga
     4861 ccagttctaa ttcactggtt ggatgttggt aactatccat aaagagagcc ccaccatcac
     4921 cggcaccaat caagaaggtc cgacggtgtt ctccatcacc actacctttt ttgcgtctgg
     4981 agtagattaa ctgccaagta atccgtggca ataaaatcaa gaaggtactc aacaagataa
     5041 agagaatgat gaaacggatg gagaagagtg gcaagaaggc ataacagata ctatatgaca
     5101 agacactgct agcagtcaca ccaaaaaaga ttttcatgaa atccgtaatc ttgctgtaac
     5161 gactaatgct cgcgttcaat ccccaaaaac caatcatcaa ttgatagaac aggaaggcca
     5221 aactcgtata gataatgtag tcaacaggtg ctggattaat cagcccataa aataaaatat
     5281 aagatacaat gatggaaacc accatactga aaatatcaaa tattccccag aaaacctgct
     5341 tttgttgttt atttaaaatt tccaccagat caatcacata atctgttagt tttttattca
     5401 tagaaatcta ctcctcctta aaagagtttt actatattgt tattatagca tatttttgtt
     5461 tatttggctt attttatcca aataaattat tgttctcttg tactccttct tccatttaaa
     5521 tcctctaatt aaacctataa tcgtcccaaa gccataagca aagtgaatgg aaaataaaag
     5581 aaagggcatc acaattagaa atccattttt atgttttaat aaagtcagca aagtgagtag
     5641 tgacaaaagt agaaaatagg cacctaatag taaagttatg aatacgaatg tgatcggtaa
     5701 caatgctaga ctaaacacaa gacttaaaac aaataaacaa ggaacatagt gaaataatga
     5761 taaacactta aactgaacat gacttgtcaa gccaatccac aaaccattta aatacttttg
     5821 atgcagcatt ttcttgaatg ttggtcgaat atactgataa gatagaatac ttgggctata
     5881 gcggatttta taaccatatt ctcgaattct ataatgaata tcattatctt cagttcggcc
     5941 aagttgctca tttactaaac caaccttctg gaaaacctct cgtttataca ttccatgaaa
     6001 aatagaagaa acatatctat cctcagaact atttcgataa ttggcaatgc tactgccaaa
     6061 catattttcc tcaacaagat gcaaggtctc tgcccatttt ccttttcctt cgacaatcgt
     6121 cggtctaggc cccccacaga caaattcacc ttgttgaata atagccacat tgttcattac
     6181 aaaactctca gtaacttttg aatgagcatc aatttttaaa ataaggtccc ctacagaatg
     6241 tttaactccc aggttaaaac cactagcttg atttttctta ggattgttat acaatctaat
     6301 tgagttaaac tctgtatctt cctttataaa ttgctgaatg atagctgtgg tcccatctgt
     6361 ggacatagca tttataaata gaatttcaat attctcttta ggataagttt gattttttaa
     6421 gtcttcaatt agaccaggca gatatttttc ttcattgtat gcactgatta caatggatac
     6481 gagaagactt tcaacatttt gagtcactac tactctcctg aatttttcct tccaccattc
     6541 tatcataatt ttcaagactt tcccattttt atcttgaaag ccagaaacct tacttgacat
     6601 tatagtgaaa aagccttaaa ataagctgtt attaaaatca gctagaagag gtattatatg
     6661 aaaaagcaat cactcttttt tgttccaggt attatcctga ttggtgtttc cttgcggact
     6721 ccttttactg ttttacccat tattttggga aatatttcgc aaggtctgga ggtagaagtt
     6781 agttcgcttg gtgtcttgac cagcctgcct ctccttatgt ttaccttttt ttcaccattt
     6841 tctacccaac tggctcagaa aatcggcttg gagcatctct tcacctacag cctcttcttc
     6901 ttgaccatcg gctcacttat tcgactaatc aatctgcccc tgctctatct aggaaccttg
     6961 atggttgggg caagtgtcgc agtcatcaat gtcctgcttc ctagtcttat ccaagccaat
     7021 caaccaaaga aaattggttt tctgaccacc ttatatgtaa cgtctatggg gattgcaacg
     7081 gctctggctt cctatctagc tgtgcccatt acacaagcca gttcttggaa aggacttatc
     7141 cttctcctca cgttactctg tctagcaact tttttggtct ggctcccaaa tcaccactat
     7201 aatcatagac tagctccaca aaccaaacaa aaaagtcaaa taaaggtcat gcgtaataaa
     7261 caggtttggg caattattat cttttcaggt tttcaatcct tgatctttta caccgtcatg
     7321 acctggttac ctaccatgtc tatccatgca ggtctatcca gtcacgaagc tggcttgctg
     7381 acttctatct tatctctgat tagcattcct ttttcaatga ccatcccaag cctgacaacc
     7441 agtttatcta ctcgcaaccg tcagctcatg ctcactctgg tttcactagc tggtgtggtc
     7501 ggcatttcca tgctcttttt cccaatcaat aatttcattt actggcttgc catccatctc
     7561 ctcatcggaa ccgcaaccag cgccctcttc ccttatctca tggtcaactt ttcactcaag
     7621 acaagcgccc ctgaaaagac agcccaattg tccggcctat ctcaaacagg aggctatatc
     7681 ctagcagcct ttgggccaac cctctttggt tacagttttg acctgttcca ctcttgggta
     7741 ccatctgtag ctgccctctt gctcatcgat atcctgatga ctgtggcact ctttacagtg
     7801 gacagagcgg ataagatcct ttaaacttct agttttagct agaagttttt tatatataaa
     7861 aattttacac atttctcttg acagggcttt cttttagatg tacaatgtat gtgtagaaaa
     7921 attatatata aaaatcttac acattagaaa aggaggtttc ccatgtactt tccaacatcc
     7981 tctgccttga ttgaatttct catcttggcc gtactggagc agggtgattc ttatggttat
     8041 gagattagcc aaaccattaa gctgatcgct aatatcaaag aatccacact ctatcccatt
     8101 ctcaaaaaat tggaaggcaa tagctttctg acaacctatt ctagagagtt ccaaggtcgc
     8161 atgcgcaaat actactcctt gacaaacggt ggtatagagc agctcttgac cctaaaagat
     8221 gaatgggcac tctatacaga caccatcaat ggcatcatag aagggagtat ccgccatgac
     8281 aagaactgaa tacctgactc agctagaact ctatctcaag aaactacctg aagctgaccg
     8341 tatcgaagcc atggactatt tcagagagct ctttgacgat gctggagtcg aaggagaaga
     8401 agaactcatc gctagtttgg gaactcccaa agaagcggcc cacgaagttc tatccaatct
     8461 tctcgataaa aaaatcaatg aagcacccgc tcaaaaaaat aaccgacaaa ttttacatat
     8521 cgccttgtta gccctccttg cagcacctat cggcattcct ctgggaatcg ccatcctcgt
     8581 gaccctgttc gcaatccttg tagccgcttt gactgtcatt ctggctttct ttgcagtttc
     8641 catactgggt atcatcggcg gattcctatt tttagttgaa agtttcacta tcctcgccca
     8701 agccaaatca gcctttatct tgatttttgg ttctggttta ctggctatcg gtgcttcttc
     8761 gctagtttta cttggcattt cctatgtaac tcgcttcttc ggtctactca ttgttcgtct
     8821 ggtacaattt gttcttaaaa aaggaaagag aggtaatcag catgcgtaaa tggacaaaag
     8881 gatttctcat ctttggtgtg gtgactaccg ttatcggctt tatccttctt tttgtaggta
     8941 ttcaatctga cgggattaaa agcctacttt ccatgtccaa agaacctatc tatgatagcc
     9001 gtacggaaaa gctaaccttt ggcaaggaag tcgaaaacct agaaattact ctccaccaac
     9061 acgcgctcac catcacagac tctttcgatg atcaaatcca catttcttac catccatctc
     9121 tttctgctca ccatgatctt atcaccaatc agaacgatag aactctgagt ctcactgata
     9181 agaaactgtc tgaaactccg tttctctctt ctggaattgg tgggattctt catatcgcaa
     9241 gtagctactc tagtcgtttt gaagaagtta ttctccagct accaaaaggg agaactctaa
     9301 aagggatcaa catctcagcc aatcgcggac aaaccaccat cataaatgct agccttgaaa
     9361 atgcgaccct caatacaaac agctatatcc tccgaattga aggaagtcgt atcaaaaaca
     9421 gtaaactcac aacgcccaat atcgttaata tctttgatac agttcttaca gatagtcagc
     9481 tagagtcaac agagaatcac ttccacgctg aaaatatcca agtccatggc aaggttgaac
     9541 tgactgccaa agattatctc agaatcatcc tagaccagaa agaaagccaa cgaattaact
     9601 gggacatctc aagcaactat ggttctatct tccaattcac aagagaaaag cctgaatcaa
     9661 gaggtacgga attaagcaac ccttacaaaa ctgaaaaaac cgatgtcaag gatcaactca
     9721 ttgcgagatc tgatgataat attgatctaa tatccacacc aagcagacgt tgacaaaaac
     9781 gcttacatct gctactatgg aagcatattt atctaactaa aaaggaggtt ctaccatgaa
     9841 acaagaatgg tttgaaagta atgattttgt aaaaacaaca agcaagaaca agcctgaaga
     9901 gcaagctcaa gaggttgcag acaaggctga agaaacgata gccgatctcg atacaccaat
     9961 tgaaaaaaat actcagttag aggaggaagt ctctcaagct gaagtcgaat tggaaagcca
    10021 gcaagaagag aaaattgaag ctcctgaaga cagtgaagcg agaacaaaaa tagaagaaaa
    10081 gaaggcatct aattctactg aagaagagcc agacctttct aaagaaacag aaaaagtcac
    10141 tatagctgaa gagagccaag aagctcttcc tcagcaaaaa gcaactacga aagagccact
    10201 tcttatcagt aaatctttag aaagtcctta tatcccctac caagctccaa aatctaggga
    10261 taaatggaaa gagcaagtgc ttgatttttg gtcttggcta gtggaagcga tcaaatctcc
    10321 tacaagtaag tttgaaacaa gtatcacaca cagttacaca gcctttctct tgctcattct
    10381 gttttctgca tcttcctttt tctttagtat ctatcacatc aaacatgctt actatggaca
    10441 tatagcaagc attaacagtc gcttccctga gcagctagct cctttaactc ttttttctat
    10501 catctctatc ctagtagcga caacactctt cttcttttca ttcctcttgg gtagtttcgt
    10561 tgtgagacga tttatccacc aggaaaagga ctggacgcta gacaaggttc tccaacaata
    10621 tagtcaactc ttggcaattc caatcttcct cactgctatt gctagtttct ttgctttctt
    10681 tgatagccta cgatttacag ctctcttgtg tgtgattagc attggaatca ttctgcttgc
    10741 cagtctccat atcattacaa gaccaagtca agcaagtgaa accgactcct tctatcagtt
    10801 attcttgtct gtccttgtga acggagttat catcctcctc ttctttgtag ctgaagtggc
    10861 actgattgga gattaccttc gtatcttggc ctttctttaa acttcatact cttcgaaaat
    10921 ctcttcaaac cacgtcagct tcgccttacc gtactcaagt acagcctacg gctagcttcc
    10981 tagtttgctc tttgattttc attgagtagc aatcctgtct ttcatggcag gattttttct
    11041 atgtcaaaaa agcaagtcga ttgacctgct tctgctttac tcttcttgtg ctaatgcttt
    11101 aaaatctttg tctaatatgg gttgcacttc tatttgattg gcgtctagtt ggtggtaaaa
    11161 agataggggt aatacctcta agaattttgc atagtccaag cgggcaatgg cttcatagtc
    11221 ttctggttta gagccatctc ctgtgatttg aaccaagtca atctcccact gcacttcctt
    11281 atccaccaat tgctcaatat aggctgcagg aacaaacggt tctctcggta aaactgtctt
    11341 gactccttga gctagatggt aaacgccatg cacttttcct tctccatcct gatagaagga
    11401 aactctcgca aagtaggcat ctgtctcttg aaccgcacgc acacgcgctt caaactgagc
    11461 caagccatct acctctaaaa agcttttcaa gttctcacga ctaaagaaaa taggattaaa
    11521 aattccttgg caaatcgtaa aacgagctac agggtctgct agataggctt gaatacttgc
    11581 ttggaaataa gcttcaaaat caaaaccatc tagcccttca atatcagtcc ctgtataagc
    11641 aagcaaagca tctaaaaatg acttgataat ctgccagtct gtctttgtta gtaggtcagg
    11701 aacatcgaca cgataagcct tttgaccatc agcataactg accttaaaga gaaattgtga
    11761 ttgccccact atcgcacact cgatatattc gagacgatta agaggctgac ggagatagac
    11821 tacatcatag ctatgtgact ccaaaccgtc tagcaaggcc aagatcgact tggcattcaa
    11881 aatctcttgt tctcctaaaa tgctctgttt atttggaata aaaaatgttt tcgccataac
    11941 tggactcctt tgaaatcgtt tacatacagt ataccttatt tttctgaaag atacaaaaac
    12001 aaactttata ctcttcgaaa atctcttcaa accgcgtcag cttcgccttg ccgtagatat
    12061 atgtaactga cttcgtcagt cttatctaca acctcaaagc agtgctttga gcagcctgca
    12121 gctagcttcc tagtttgctc tttgattttc attgagtatt agatttttga gtacaaagca
    12181 tagaaaaaca gcccctaagg actgtttgat cttatacagt agctgcctga tccaagcttt
    12241 caccgatagc ggctaggcgc tcaatcactt cagcttgtgt caattcattt tttgaaacat
    12301 agcggttacg tgggtgaaca cggcactcgt gtgagcatcc acgaaggtac ttgtcttcat
    12361 tttcttctga tgtcaagata cgacggttac agaagggatt tccacagttg acataacgtt
    12421 cacatggtgt tccatcaaac cagtctttcc ctacgatagt tgggttgaca tggttgacat
    12481 caacggcaat acgctcgtca aagacgtaca ttttcccatc ccaaagctca ccttgaactt
    12541 ctgggtcttt accgtaagtt gcgattcctc cgtgcaattg gccgacatct ttgtagcctt
    12601 cacggaccat ccagcctgag aatttctcac agcgaacgcc acctgtacag taaaccacga
    12661 cacgcttgtc catgaatttt tctttgttgt cacggaccca ttgtggtaac tcacggaagt
    12721 tgcgaatatc tgggcgaata gccccacgga aatgtcctag gtcgtactca taatcgttac
    12781 gtgtgtcaag gacaacggta tctttatcaa gaagtgcttc tttgaactct tttggagaca
    12841 agtaagcacc tgttgtttca agtgggttga tgtcattgtc aaagtcgttg tcttccaaac
    12901 caaggtggac aatttctttc ttgtagcgaa caaacatctt cttgaaggct tgttcatttt
    12961 cttcgtcaat cttgaaccaa agttcttcca ttcctggaag gctgtgaacg tagtccatgt
    13021 atttttgagt tgtttcatag tcacctgaaa ctgttccgtt aattccctcg tcagcgacta
    13081 ggatacggcc tttaaggccg attgatttac agaaagccaa gtggtctgca gcaaattgct
    13141 ctgcattttc aattggagta taaaggtagt aaagtaagac acgaatatct tttgccataa
    13201 gatttgttct cttttctatt cttaaatttt ctaaattttt tatttatcta cactagtata
    13261 cctgcttgag aaaacgaatg caatttattt gactggaaat tgtagagggt tctcgaaatt
    13321 tcctgaatgg ttaaaataag gacaagagaa aacatggata tctatatcct tgtgccaaaa
    13381 aaaccactgc cctccccaga ccaacctgag gaaagcagtg attcttattt taggagttag
    13441 gaatgaatac acgaaatcaa tttagctgat tattttttgt ttttcaagaa ttcatcgtat
    13501 tgtttttgca tttcgttcaa tactttttcg taggcacctt cagatttcaa tttttccatc
    13561 aattctggaa tcgctttatc tgggtctaca gtaccagtgt tgatagctgt atcaaattgt
    13621 tgcattgtgt tagcaatagc tgagatttca gatttcacat tgtcagtatt gaagataaat
    13681 ccaagcgctg gagattcttt agcttctgcc aattctttct tagaattttc gatttgttgg
    13741 tctgtaacgt tttcgttgat gtaaaggatc cagttgttac cagtgttcca tccacccatg
    13801 tgagtgtttc ctttgtagcc atcaagaacg cgaacacggt tttctttacc ttcaattttt
    13861 tcccagttct tgccttctgg accgtaaaca agaccgttca agagttctgg gttcgtattc
    13921 aagaggttca agatttccat tgatttttct ttgttcttag agttgtttga gatgacaaag
    13981 ttagcaactt gtgttgtttg gtttttcttg atgaagttag taattggttt gatttggata
    14041 tctttgttgg caacacgtga aagcaagctg ttaccgtagt cagctggtcc tactgtttct
    14101 tcacgaacga accaagtatc ttgttgaagg tcaaatgaag tatcgcttgt tgcgacatct
    14161 tttggaatgt agccagcttc atagaatttg tgaagagtct tcaagtgttc tttgaaacga
    14221 ggcacttcgt aacggtttac aactttagta gtatcgcctt caaggtcaat aacgaatgga
    14281 agcccgtttg ctactgggta gtcaaagtta tcagatggga tgaaaacttt accaatagca
    14341 aatggtacta cgtctggagc tttttcttta atttgtttca agactggctc aagagtttcg
    14401 taagaagtaa cacctgaaat atcgatacca tatttggcaa ggagagttcc gttgaaggca
    14461 aagttttgag atgatgcaac gttggctgca actggtacag cgtaaatctt accatttaca
    14521 gtattaccct tgatgtaagc tgggtcaagt gccttgtaaa ggtctttacc ttcttttttg
    14581 tacaattctg tcaagtcagc ataagcacct ttttgagcat ttacaatata gttatctgca
    14641 aaggcgatat catagttttc accagatgat gtgataactg acattttctt accatagtca
    14701 ccccagccaa ggtattggat atccaattta gcaccaactt tttcttcgat gattttgttg
    14761 gcatttgcta acaattcatc caagttatct ggtttgtcac cgatttggta cattttgata
    14821 acaggtttgt cacctgaatc agcagctttt ttgctgttac ctgtcaaatt tccacaagca
    14881 gcaagacctg cagccagagc gactacacta gcagatgcaa aagcatattt tttccagttt
    14941 ttcatgataa aaactccttt ttttattttt aaacttataa acaatgtaat gatcttaact
    15001 tcacgcaagg gaagtttgga aacaagtgct attctttcac accaccgata gtcaaacctt
    15061 ttacaaagta gcgttggaag aatggataca aaatcgcgat tggaagggtt gctaccacaa
    15121 ccatggccat acgacctgtt tctttcggta gagcaactcc cagttgacca gtcaagccga
    15181 ccgctttggc aatgtagtcc atattttgtt ggatttgcat gagcaaatat tgcaatggat
    15241 acaagttgtc actcttgatg taaagaaggg cgttgaacca gtcattccag aaaccaagag
    15301 ctgtcaaaag cgtgatggtt gcgatacctg gtagtgacaa tggcaaacag atttggaaga
    15361 aaatccgggc ctcactggca ccatcgatac gagccgattc gagaatggct tctggaatgg
    15421 tcttcttgaa gaaggaacgc atcaagatga tgttaaaggg tgagagaagc attggcacaa
    15481 tcaaggccca aacagtgtca ccaagctgaa gtacacgggt caccatgata taacctggta
    15541 ccaaaccagc gttgaacaac atactgagaa ggacgaagat ggtaaaaaat ctgcgatact
    15601 taaaggttgt ccgtgaaata gcgtaggcat aggttgttgt gataaagaca tttgtcaatg
    15661 tcccaactac ggttacaaag acagagatga agagggcttg taggatttta tccttaaact
    15721 gtgccaaaaa ctcaaaaccg tctaagccaa attgggatgg gaagaagcta tagccgtatt
    15781 ggaggaggct tttctcgtct gtcactgaaa taatgataac gaatacaaat ggtaggatac
    15841 aagagagggc aatcaaaccc gaaatgatac tgaagaagat atctgctttc ttactgaagg
    15901 agtgaatgcc gacattatca attttttctt ttttaatttt cttttttgcc atattctcct
    15961 cctttctaga acaaagctga gtttggatcg actcgtcttg caagcaagtt tgataggata
    16021 accagaatca aaccaacaac ggattggtaa agaccggctg ctgcagccat accgatatct
    16081 gctgtctgag tcaaaccatt aaagacatat acgtccaaaa cgttggttac attgtaaagc
    16141 tgaccagcat tgtgtgggat ttgatagaag agaccgaagt ctgcgcggaa gatatttccg
    16201 actgcaagga tggacaatac agttacaagc ggagtcaact gaggaatggt tacgttgcga
    16261 atacgttgcc acttgctagc tccgtccact gtcgctgctt cgtagtaggt tggatcaatt
    16321 cccatgatcg tcgcatagta catgacactg ctatatccaa aacctttcca aatacctagg
    16381 aaaagtagga gatagggcca gatgcccagg tcagcgtaaa aattgacttc tttgagacca
    16441 agactttcca atagatgatt gaacacccct ttatcaatat ttaggaaggc atctgtaaag
    16501 aaactgatga taacccaaga caagaagtaa gggaacaaca tagaagtttg gaagatcttc
    16561 accattctct tagaacggag ctcgctgagg ataatggcaa tccctacaga tacaactaaa
    16621 cctagaaaga taaagccaag attgtagagg acagtatttc gtgtgataat aaaggcgtct
    16681 cttgaactaa ataagaatct gaaattatcg agtccgaccc atttactatt tatgatacta
    16741 tctatgaaac cattactggt catgtggtag tctttgaagg caaccacgtt cccaaatact
    16801 ggaatgtaaa agaatagaat caaccagagt gcccctggca aaaccatcaa gagaaagatc
    16861 cagttgtctc tcaatgtttt tgaaaacttt ttcataattt cctccctttt tattttgata
    16921 tccatctaaa aattcttttt tagacttttg ataacgatta cattattagt atactcctat
    16981 ttgcaggtta ggttaaactc ctaattatag aaaaaactcc acaaattatg tagcagattt
    17041 aaaactttat caccactatc aaacaaatgt cctaaatcaa ttgtttattt tatctctatt
    17101 agcccagtga tggcgtcact ctgttataag catccaacaa cggggtatac tgaaaaatct
    17161 ccagactagg gaactcagcg atagttccta atctggagat ttttaatatg ttattaggcg
    17221 tttgctttca acttagcaat aacctcttta agattatcaa tcaactctgc tgcagtatgc
    17281 tcagagcctt tttcatctgc caagaacaaa actgcttttt gaagttcttc ttgagagttt
    17341 tcaaggacat ccttatctac tgtttcaagg tttgagtctt taagaagttt acttaattcc
    17401 ttggctaatt tcttgagttt gatttgcaga ctcatcttct cctgctgttt ctttgcccgc
    17461 tgtttgtcct ccatccttag ttgctgactg gctttcctta atggactcta gggaagcaat
    17521 ggcatctttg actgtttgca agatatcacg taaaccttgc tctatcaaac tatcatctgc
    17581 aaaagatttc ttagcctctg ccaaaaccag aggtgctgaa tctgtggtag gattcgatac
    17641 acctgtcaat gatctcaaaa gattttctaa ggtttgagtc tgcttactaa tgctagacta
    17701 aaatcaaaaa gcattata
//
LOCUS       DAVKAO010000036        17316 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKAO010000036 DAVKAO010000000
VERSION     DAVKAO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.036"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(356..1112)
                     /locus_tag="VWX16_001283"
                     /pseudo
     CDS             complement(356..1112)
                     /locus_tag="VWX16_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(1250..2872)
                     /gene="groL"
                     /locus_tag="VWX16_001284"
     CDS             complement(1250..2872)
                     /gene="groL"
                     /locus_tag="VWX16_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9158629.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2888..3172)
                     /gene="groES"
                     /locus_tag="VWX16_001285"
     CDS             complement(2888..3172)
                     /gene="groES"
                     /locus_tag="VWX16_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9158630.1"
                     /translation="MLKPLGDRVLLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3328..3723)
                     /locus_tag="VWX16_001286"
     CDS             complement(3328..3723)
                     /locus_tag="VWX16_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9158631.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3801..4562)
                     /locus_tag="VWX16_001287"
     CDS             complement(3801..4562)
                     /locus_tag="VWX16_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9158632.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFLLDVTDMVMVKTVCSDILETIGQIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(4604..5230)
                     /locus_tag="VWX16_001288"
     CDS             complement(4604..5230)
                     /locus_tag="VWX16_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9158633.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(5246..5563)
                     /locus_tag="VWX16_001289"
     CDS             complement(5246..5563)
                     /locus_tag="VWX16_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9158634.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLTKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(5560..5859)
                     /locus_tag="VWX16_001290"
     CDS             complement(5560..5859)
                     /locus_tag="VWX16_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9158635.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(6144..6335)
                     /locus_tag="VWX16_001291"
     CDS             complement(6144..6335)
                     /locus_tag="VWX16_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9158636.1"
                     /translation="MSYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGAMIANDWVKDNGK"
     gene            complement(6496..7170)
                     /locus_tag="VWX16_001292"
     CDS             complement(6496..7170)
                     /locus_tag="VWX16_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158637.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(7176..7622)
                     /locus_tag="VWX16_001293"
     CDS             complement(7176..7622)
                     /locus_tag="VWX16_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9158638.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7736..8239)
                     /locus_tag="VWX16_001294"
     CDS             complement(7736..8239)
                     /locus_tag="VWX16_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9158639.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(8236..8598)
                     /locus_tag="VWX16_001295"
     CDS             complement(8236..8598)
                     /locus_tag="VWX16_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEU9158640.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICSVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8664..10523)
                     /locus_tag="VWX16_001296"
                     /pseudo
     CDS             complement(8664..10523)
                     /locus_tag="VWX16_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10562..11011)
                     /locus_tag="VWX16_001297"
     CDS             complement(10562..11011)
                     /locus_tag="VWX16_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9158641.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(11135..11851)
                     /locus_tag="VWX16_001298"
     CDS             complement(11135..11851)
                     /locus_tag="VWX16_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9158642.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12732..14147)
                     /gene="ply"
                     /locus_tag="VWX16_001299"
     CDS             complement(12732..14147)
                     /gene="ply"
                     /locus_tag="VWX16_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9158643.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(14158..14490)
                     /locus_tag="VWX16_001300"
     CDS             complement(14158..14490)
                     /locus_tag="VWX16_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158644.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14561..15169)
                     /locus_tag="VWX16_001301"
     CDS             complement(14561..15169)
                     /locus_tag="VWX16_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158645.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(15182..15640)
                     /locus_tag="VWX16_001302"
     CDS             complement(15182..15640)
                     /locus_tag="VWX16_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9158646.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15765..16161
                     /locus_tag="VWX16_001303"
                     /pseudo
     CDS             15765..16161
                     /locus_tag="VWX16_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16919..17179)
                     /locus_tag="VWX16_001304"
     CDS             complement(16919..17179)
                     /locus_tag="VWX16_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158647.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELELVKSTILQNDDISLDQNEIFEILKNFFDG"
BASE COUNT         5091 a         3905 c         3121 g         5199 t
ORIGIN      
        1 ccgttatagc agagttgtaa cacatttcta taaagaaatt tattcctacg actcagtttg
       61 gcatgcttaa cttcctaact caaaaacaaa gcatcgatta atttatccgt tcttcttttg
      121 aaacagattt gaactgataa ttgctcgttt taatttaaaa agctcttttg agcaaaactt
      181 cattttttta agagtccaac aatttctgtc tatcgctttt ctactagcaa aaaacctacc
      241 ttgaggcaag ttttcattta tatatctaat ttcgtaacag tatagtaaaa tgaaataagg
      301 acagtcaaat cgatttctaa caatgtttta gaagtaaagg tgtactattc tagtttcaat
      361 ctactataaa tttgatatga gaatagtctt tctcaacatc cttatccaac aagcatgctg
      421 tggcgtcctt cttaacttaa accaggtcaa tattctgggc ttgggctgca tagacgcagc
      481 cacaataaca ttgacgatag atatcatact cctcacacat ctctactgaa cgtttgtagc
      541 cttgattttt cttgaaatcg ttgggaagat agtggggcgt gtaaattttt tgcacatcga
      601 ttccgatgct attgatagtt tgagaattct tatgaggact gatggtcaag gctgaaccaa
      661 agtagtcaaa gcccaagtcc atagccactt gcgctgtttt atccagtcgg tagtcaaaac
      721 aaaccttgca acggtcgcca ccttcgggct cctcctctag ccccctaact agttttcggt
      781 attaattggg ttcgtaggga gcttctaggt actgaaccgt atttcctgtc cgctcattaa
      841 aatcactaac aaatttcttg gtgacataga cccgcttatg gtattctgcc ttgggatgga
      901 tattagaatt ggcaaaatag atggtcacat ctgcatactt ggtcaaatat tctagtgtat
      961 aggtactaca aggggcacag caaacatgca cgagaatggt tggccgctac tcattttttt
     1021 cccatgcttg taccattttc tgcatgacac ggtcataatt aatcttctga ttggggggtc
     1081 atcttgcaca gaatttcttc tacatcgatc atgttcttct cctttttcta gtcttatttt
     1141 atcatataag ttaggagagc tcaaatcaat ttagaagtga atattataaa aagaagggtt
     1201 agtcattccc tccttttgtg ttttttataa gttgttttct atagaaagct tacatcatcc
     1261 cgcccatcat acttggatcc attgctggag ctggggctac tggttctggt ttattggcta
     1321 cgactgcttc tgttgtcaaa atcaagctgg ctacagatgc tgcattttgt agggctgaac
     1381 gactcacttt aactggatca atgatacctt gatcaatcat gttaacccac tcgccagttg
     1441 ctgcgttgaa tcctatacca agctcagcat ttttcaaacg atcgataacg atagatcctt
     1501 caaatcctgc attgtgagca atttgacgaa cgggttcttc caaagcacgg agaacaatat
     1561 tacgtcctgt tgcttcatct cctgtcaatt ccaaggtagc aacagctgga atcacattgg
     1621 caagagctgt tccaccacct gcaacaatac cttcttcaac agctgcacga gtagcgttga
     1681 gggcatcttc aatgcggagt ttcatttctt tcaactcagt ttcagttgcg gctccgacct
     1741 taataaccgc tacaccacct gacaatttgg ccaagcgttc ttgcaatttt tcacggtcaa
     1801 attcagaagt tgtagtttcg atttgagact tgataaccgc aacacggtga gaaatcgctt
     1861 caggatttcc tgcaccttct acaataaccg tgctatcttt gtccacggtc actctcgctg
     1921 cttgaccaag agcttcaatt gtcgcatctt tcaactcaag accaaggtct tctgtgataa
     1981 ctgttccgcc tgttaagatg gcgatatctt caagcatggc tttgcgacgg tcaccaaaac
     2041 caggtgcctt gactgctact acgttgaagg ttccacgaat cttgttcaaa acaagagttg
     2101 gaagagcctc accatccaca tcatccgcaa taatcaagag tggacgattg ctttggagaa
     2161 tgctttccaa aagtggcaag atttcttgga tattggaaat tttcttgtct gtaatcaaaa
     2221 tgtacggatt ttcaaggtca gccaccattt tttcgctatc tgtcaccatg tactgtgaaa
     2281 ggtaaccacg gtcaaactgc attccttcta cgacttcaag ctctgtttcc ataccacgtg
     2341 actcttcgat ggtgatgaca ccgtctttgc caactttttc cattgcttca gagatgtact
     2401 caccaacttt ttcagaacga gaagatacgg ctgcaacttg agcgatagct tctttattgg
     2461 caacagggat ggcgttgttt ttcaaagctt caactgctgc ggcaactgct gtttcaatcc
     2521 cacgacgaat accgattgga tttgcacctg ctgtgacgtt tttgattcct tcacggacga
     2581 tagcttgggt caagactgtt gcagtcgtag ttccgtcacc tgcgatatca ttggttttag
     2641 aagctacttc tgatactaac ttagcaccca tattttcaaa atggtcttcc aattcgattt
     2701 ctttggcaat ggtcacaccg tcattggtaa tcaagggtga accgaatgac ttttcaagaa
     2761 cgacattgcg accttttggt cccaaggtta ctttaacagt gtctgcaaga atatcgacac
     2821 cacgaaccat ggctgaacgg gcatctgatg aaaatttaat ttcttttgac atacttactt
     2881 tctccttcta ttcctcaatg attgccaaaa tgttagcttc gcctacgatg atgtactttt
     2941 catcgccatc tttgacatca agacctgcgt gggcttcaac taagacacga tctccagttt
     3001 taacacttgg agcaaccaag tcaccgttca aggtacgaac accttgtcca gtagccacaa
     3061 cttgagctgt tttggttttt tcttgggctg agcctgcaag gacaaagccc ccaacggttt
     3121 gttctttttc ttctattttt aagagcacac ggtcccctaa tggtttcaac atctgatttc
     3181 ctccataatg agatagatat tattagcact ctttgaactg gagtgctaat tcataattct
     3241 attgtatcac ttggtcagaa atagtcaaga aaaaagtctg actttctcaa gataaaaagc
     3301 ctgagaccaa ctcagacttt ttaattctta aaatggcaat tcttcctctt ccaagaccaa
     3361 atctgccaaa tcttggcctg cattattttc acgcatagca cgttgggcac gactttccaa
     3421 gagttggaat cctgtcacaa ggacttcggt tacataattc atctgaccat ttttctcaaa
     3481 gcgacgggta cgcaattctc catcaacgga aatgagacta cctttggttg cgtagcttgc
     3541 caaagtttct gctagtctgc cccatagaac catattgaca aaatcagctt cacgttcacc
     3601 gttttggtct ttgtaacgac ggttgacagc gatagttgct cgcgctaccg acttgtcatt
     3661 gttggttttg tgcaattctg gtgtagacgt taaacgccca atcaagataa ctttattata
     3721 catattttct tcctcctact tatctattcg taggaaatca aaaaaagtta cagaaatttg
     3781 taacttttcg agaaaatttt ttatttttta tgaaccatga aacctgtcgc ctgttgattg
     3841 gccataatgg tcatatctgt aatctgaaca cgacgaggtt gactagtcac atagactact
     3901 gtatctgcaa tatcctgagc ttgcaaagct tctattcctt ggtaaacgga cgcagctcgt
     3961 tctttatcac catgaaaacg cactgtagaa aaatctgttt cgacaattcc aggctgaatg
     4021 gtcgtcacct tgatatccgt tgcgatggta tcaattcgca gtccatctga aaaggtctta
     4081 actgccgcct tggtggctga gtaaacagct gcaccagcat aggcataaat tcctgcggtt
     4141 gaccccatat tgataatatg accttgattg gcttttacca ttgctggcaa gaaacagcga
     4201 gtgactgcca tcaaaccttt gacattggta tccaacatgg tcagcatatc caactcttca
     4261 tagtcttgat agggagctaa gtcaagagcc agtcctgcgt tattgaccag gatgtcaatc
     4321 tgacctatcg tttctaaaat atcagagcag acagtcttta ccatgaccat atccgtgaca
     4381 tctaggagaa aagtccaaac tgtttgattt ggaaaagttt ctgcaaactc cgccttaaga
     4441 gcttctagtc tgtctatccg tcgtcctgtt agaacgacat cctcaccctg ctccagataa
     4501 gcacgcgcaa tcgcttcacc gattcctgat gtcgctcctg taatcacaac attttttgcc
     4561 atcttatttc cttctagctg gtctatcaga tattaacaac ttcttaggca gtccagtgtt
     4621 tagctgagtc aaatggtgtt ccgacaactt ggtcttctga taattcaagc accccacgtt
     4681 tttgtggagc atttggcaga tgcaattcac gaggactgca catcatacca aaactctttt
     4741 caccacgaag ttcacctggg aaaatgagat tcccttttgg catcatagct ccaggaagcg
     4801 cgacaatggt tttcaacccc acacgcgcat tgggagctcc tgcaacgatt tgtacagtct
     4861 tatcacttgc gactgcaact tggcagatgt tgaggtggtc actatctgga tgggctacca
     4921 tctcaacaat ttcaccgaca acaaacttag gttccttatc attaacaatt tcttctgtaa
     4981 aaccttccgc ctgcaactct tggttcaaac gagcgacttg ctcatctgtc aaaaagactt
     5041 gaccgcgctc tgcaatttca aataaacttg aaacttcgaa aatattccaa gccactgttt
     5101 ccccattatc tttgagaaaa acacgggcta ccttgccttt gcgctccaca tccagtttgg
     5161 catctccgct atttttcacg atgaccataa ggacatcacc gacatgttct ttattatatg
     5221 taaaaatcat tgtttccttt ttctcctatt tcagtcctgc taaaaagtca ttgatttgtt
     5281 gcttgctttt acggtcgcga ttgacaaaac gaccgatttc cttgtccttt tctagaacaa
     5341 caaggctagg aattccgtaa acatcccaga gtttggtcaa atccatatac tgatctcggt
     5401 ccattcgaat aaaggtgaac tctggattgg tctcctcaat ctctggtaag gcaggataaa
     5461 tataacgaca atcgccacac cagtctgcca caaaaaggaa gaccttcttg cccgcttttt
     5521 ccactaaaga tgctaattct tctaaacttg ctggctgtat cataagactt cctcctcata
     5581 gactaggtct tcattttcat agacaaaggt ataatgacgg ccatcctcaa aaatgacgcc
     5641 accaaccaag ctctccagac tgctttcgta aacttgaaca tagagggtcg caatttcccc
     5701 catgtcggaa aaatggtctc gcacaatctc tgtcaactct tcctgagtct tcatgagctt
     5761 acggtcatct gcaacttttt tcgtagcaag agcaaggctt ccgataccta gcagagccaa
     5821 gcctgccatc cacatttttt tagctttcat accattcatt ttaacacaaa aaaggcttca
     5881 ggacaaatga ggaagcagca gaaaagcaag taaaaagcct cttcctttaa ggaaaaggac
     5941 ttcttatact aagagcaaac tagaaagcta gccgcaggct gctcaaaaca cggttttgag
     6001 gttgtagata aggcgacgtt gacgtggttt gaagagattt tcaaagagta ttattcttat
     6061 tgccaggcac ctaagttgcc aacgtagtaa ccatcaggtg tgtaggtatt gcgaagcatc
     6121 ttacctgatg aagccagata atactacttg ccattgtctt tgacccaatc attcgcaatc
     6181 atggcaccag aagaacttac ataataccat tctcccttgt cataaaccca agtactgact
     6241 ttcatggttc ctgagcaatt aaaggcaaaa aaactgtcca atatcattcg ttttttaaaa
     6301 gcatttgaca ctacatcatc tacgctgtag gacatagata catcaaacgt aaaagccaaa
     6361 atatcgaaaa atatacaact tttaaaggca gcatttcatt ttttaaagtc taacaaccat
     6421 agaactgaaa tcagagcaag gcatcaattt cctactctta gtacgtctat tttgatggtt
     6481 gaagtgaaga tttttttatt taacgtagac aatttccaac ttcccaaaag ccacatctcc
     6541 acggataatc aaggttttgc tggttggggc tacaggagcg tcagccttag ctgcaccaaa
     6601 ggaggtttct actttcaaat ctacacgcca gtgttgtgga acatagacgg ttgcatttcc
     6661 gaaggccact tcaatattta aagttgcaaa atcacctagc atctctgcat tatcatagta
     6721 gattttagca tccccaaaag caacttccac ttggtcatct acgagatctt gatcttgctt
     6781 atagaaggtc ccgctaccaa aagcgacttc cttatccgtg acgactgttt ttttcccatt
     6841 gtaccaccat tttttttcat tccagaactt acttgaatgc gtcagataac caacaccaag
     6901 taccaccaag atgctagccc aaataagaga atgattggta actggtaaca agtcataagc
     6961 gtaatttgca atgatgagcg ccagtaaacc tgtaaaaact gccgaagtga gatgacgtct
     7021 aaggatggac tcaatggact tataagcaaa aaaaacaata cctagtaaag gccatatttt
     7081 accatccaaa gaaggaattc caaaattccc ttgcagcaag atccaagctg ctaaaaccaa
     7141 taaaacaata ccaaatgctt tctttttcat gttttttacc tcatgtttct tagattttct
     7201 tttaggaggg attggtaatg ccgtgagaca tgaacctcct tgtgcgtctg ataaaaggaa
     7261 atggtgcccg ttcctgaaaa ggacttgtcc actgagtaaa tctgacggat gtttgcgatc
     7321 gttgacttgg aaactcgatt aaaatagcga ggcaagatag actccagctc atagagcttg
     7381 aggcgaacct cataagcttc cttctggtta tgagcgtaga tcttgctccc ttctgtttca
     7441 aagaagagaa tttctgacag gtctagataa tattcacctg tccccttgta aaaagtcaac
     7501 ctcggagcct tggattcttg caagagccgt tgcaagtctg caatttcatc tgtcaaagtg
     7561 ggggtcttga tgacaatttc agtctcctct aaattgccgt caatctcaat tcgtaacttc
     7621 atcacatctc ctttctgact ctactatatc aaagctaagt taggaataca agggcaaaaa
     7681 agcaagtggt caatttgaag cagtaagtgg ttgtaggact agcttaactt acatgtcaga
     7741 ataggaagaa aaggaatccc cacaccaaac cacaagcata gccaaccaag acatccttgg
     7801 gataatgaac accccctaaa accctcacca accccagcaa agctgaaaag atcaagcata
     7861 ctaagccaat aggtagacta gcatgcagac aggccatgga gatgatagtt gccgaaaaga
     7921 catggcgact gggcatcgac cgtcccgaac tatccttatc aagaagggga cagatatccc
     7981 aagtttcata aggtctcggg tgattgattt tcttacgaaa cagggacaag gccacaaaac
     8041 ctgatgcagg aataagcaca taaaccccaa cttgctgacc aagtcctagt tgcaggtaag
     8101 tggtgactag caagatcaga tagaccaggg gcatggcgac tgtcatcaaa cgattgaaac
     8161 tgcgtaatag gaataaaaga attggacgac tagttagtct ggagctgata tttcgatacc
     8221 attcttgata atttttcatt tatgcttctc atacttttct agaacttgtt gagatgtttt
     8281 ccccttggca agccaatcca ctagcttgtc caagcgacgt atttcctgca tcagaggatc
     8341 atcaatttcc tcaatacgga cactgcaaat cttccctgta attgcaattc gttcagggac
     8401 ataatatggt gcttgtctaa aaaaatctcc gtaagtaaca tccctatcca gacaagcaag
     8461 aacatcactc acttcatacc ctgtcaacca actggtcact tggtgaactg actctgcctt
     8521 tccacccttt cgctcaactt ttgcaatcaa ggctaagtag acagcagcaa atttcatatt
     8581 gtataatttc tgactcatac ttctctcttt ctatgtgtac tagtataaat aagaaaaaga
     8641 aggccgtcaa gccttctttt gatttattct tctgcttcat cttctgtaaa ttgactattg
     8701 tacaagtcag cgtagaagcc accttgcgcc atcagttcct catagttgcc ttgctcgatg
     8761 atatttccat ctttcatgac caagatcaag tctgcatttc ggatggttga caagcggtgg
     8821 gcaatgacaa aggatgtgcg tccttccatc aaacggtcca tggctttttg gatcaattcc
     8881 tctgtccgtg tgtcaacaga agaagtcgcc tcatccaaaa tcaaaagcgg tgcatcctta
     8941 agaagggcac gagcaatagt caatagttgt ttttgtccta cagacaaggt cacggtgtca
     9001 tccaagatgg tatcatagcc atctggcaag gtcataataa agtggtgaat tcccacagcc
     9061 ttactagctt ccatcattcg ttcatcacta atccctattt gattatagat gagattgtct
     9121 cgaatagttc cttcaaagag ccaggtatcc tgcaagacca ttgaaaaggc atcatgcact
     9181 tctgaacgcg tcatagcctt ggtatccaca ccatcaatgt gaatacttcc cttatcaatc
     9241 tcatagaatt tcatcaaaag attgacaatg gttgtcttac cagtcccagt cggcccaaca
     9301 atggcaacct tttgaccagc atgagctgtc gcagagaagt catagtcttg aacattgaca
     9361 ccgtccacca gaatttctcc tgctgacacg tcgtagaaac gtggaatcag attgaccaga
     9421 gttgatttac cagaacctgt tgacccaata aaggccactg tttgaccagt ttctgcttta
     9481 aagctaacat gttcaataac tgcctccgaa tttgccgcat agcggaaggt cacatcctta
     9541 aactcgacct gacctttgaa gttttcatca gtcagctgca cttgaacagg gttttggata
     9601 gaagaatgca aatctaaaac ttgattaatc cgcttagcag agaccatagt tcggggaaga
     9661 acgatgaaga gtgctcccat gagaaggaag cccatgacaa cctacatagc ataagacatg
     9721 aaaacaatca tgtcactaaa gagaggcaga cgcgctatcg gagcagcgtc gttaatcaca
     9781 taggccccaa tccagtaaat cgccacactc aaaccacttg aaatccccat catgatagga
     9841 ttcaaaatag ccataagatg gttgacaaac aaattttaac gggtcaattc atcatttact
     9901 gctgcaaatt tttcattttg ataatcctct gcattgtagg cacgaacgac acgaatacct
     9961 gttaaactct cacgagtgat actgttcagt ttatctgtca gcccctgaat caaggactgt
    10021 tttggaaagg ctaacgtcat caaaacggtc gtcatcagga cgttgataat cactgccaca
    10081 agtacggccc agagccagta ttctgaatga cctaaaatct tcccaatagc ccagatagcc
    10141 ataattggac cacacgttac cacttgcaag cccatagtaa tcaacatttg aacttgagta
    10201 atgtcattgg tagtacgcgt caagaggcta ggaattgaaa atttcttaat ctctgtctgc
    10261 gagtaatcca aaactcggtt aaaaatatca cttctcagcc tactagtata agaagccgcc
    10321 actcgggatg caaaaaatcc aactgcaact acggacaaga aggcaagaaa ggacattccc
    10381 atcatcatgc ttgccggctg ccacaactca tctaaattag tttcttgact acctagcaaa
    10441 tccgtaattt tcgagatata ggtcggcact ttcaactcta gatagaccaa aaagcaagta
    10501 aagagaatgg ctagtaaaat catcccccat tcttttctac taattctttt ggctaatttc
    10561 tttattctct cctcctattc ccttgatatt ttgcctgtag ttgaccgaga accttctcaa
    10621 aaatcagtaa ttcatcttca tcaatgtctt ccatcaactg cttgtctatg cgttcaaaaa
    10681 aagccttaac ctgttgcatc tgagaacgtg ctttgtccgt cagacgaaca aacttagccc
    10741 gcttatcaac aggactcgcc tccaattcca ccaaaccatt ttgcactata cgcttaacca
    10801 gattactagc aacagacttg gtaatattga gttcctgctc gatatcttta atcaagacca
    10861 agtcttggtt tttctcgcga ttatccaaaa aatgcacaac ctgaccttgc ggcccaccca
    10921 taaattcaat gccgcaacgt ttggcttcct tttgcaccat caggtgaatt tgatgaccaa
    10981 aacgcttaaa gactaacatc ggtttatcca taatctcccc cttctaaata aaaatagttc
    11041 tctggagaat aattaaattt ctatgagaac tattttcttg attaaaaaaa tcccaagtga
    11101 ttttctcact taggatcatg ttctataggt taaattaaaa cccatctacg ttcgtataaa
    11161 tcttttggac gtcttcgtcg tcttcaagaa cgctgtaaag tttttcaaag gtttcaaggt
    11221 cttcgcctga caattccact tctgactgag gaatcatttc caattcagtc acttggaatt
    11281 cttcaatacc agactcacgg agggcaacga tagccttgtg aaggtcagtt ggcgctgtgt
    11341 aaactgtgat tgtaccttct tgtgcttcta cgtcatccac atccacatcc gcttcgagca
    11401 attgctcaaa gactgcgtcc gcatcttcac ctccaaatac aataacacct ttgttgtcaa
    11461 agaggtaaga aacagaacct gaagcgccca tgtttccgcc gtttttacca aaggctgcac
    11521 ggacattggc tgctgtacgg ttgacgttag aagtcaaagt atccacaatt agcatagagc
    11581 catttggccc aaaaccttcg taacgtcctt ctgtaaaggt ttcgtctgtg tttcctttgg
    11641 ctttatcaat cgctttatcg ataatgtgtt ttggcacttg ggcttgttta gcacggtcga
    11701 taacgaattt caaagctgag tttgattctg gatctggatc acctttttta gctgctacat
    11761 agatttctac accaaatttt gcatatacct tagagttagc tccatcttta gccgttttct
    11821 tggctacgat attggcccat ttacgtccca ttaggaatct ccttttttca cattttaatc
    11881 tttattatta taacacaagt ttttttgatt ttcactagag gaaatggatt ttattagcaa
    11941 atcaagctag gatagcactt tacctgctaa gatggtctta cctttctatc tttatcaaca
    12001 gacactcatc cacattcaaa aaacaaacta gaccattatc tgcaaataga aagtttcagc
    12061 caagtttgac aaagtcagct caaattactg tttgaagttt gtagatataa gcgacaaaaa
    12121 caatcatact gcaccttttg ttgacagtct actccagaca tatcatagtt caagtaaata
    12181 ctttgaaatc caacagttct tataggcgct attgtattct aagaaatcaa tagaagagtt
    12241 tctaagcaaa tctctaatac tcaataaaaa tcaaagagca aactagaaag ctagcctcag
    12301 gttgctcaaa acactgtttt gaggttgcgg atagggctga catggtttga agagattttc
    12361 gaagagtata atttacgtgt tcccaagatg gagaagttag actagtacac tggcacttct
    12421 aaaccattgc tagcaattga tttgttcata tttaatttca ttttttccat aaatgggtat
    12481 tagatataaa cagcaaaata tttccgatac gtgtcgttct tgaatttcca atcatctaaa
    12541 acaagtaaag gataatcaat cccctgtata tcaaggaatt ggctaccctt tttacttttt
    12601 tacacattct gtttgataga ttcattttaa catcacgagc atactccaat ggaaatcgct
    12661 aggcaagaga taaactttca gatatccgca gagagatcat cgcctctttt tgtcgcaagc
    12721 attctcctct cctagtcatt ttctacctta tcttctacct gcggatagag agttgttccc
    12781 caaatagaaa tcgtccgctt acgcactagt ggcaaatcgg ttttttcata aaccgtacgc
    12841 caccattccc aagcaagccc ggtacactct ctaattttga cagagagatt acgaacattc
    12901 ccttttaaag gaatactagt ggtaaagtga gccgttaaat cctgcccatt tctgtcccaa
    12961 gccttaggag tcaagacttc cttaccttga tgatcatagg ataattcatt ccaagtaata
    13021 taatattggg caacataggc accactatga tccagcagta aatctccgtt tctgtaagct
    13081 gtaaccttag tctcaacata gtctgtacta ttttgaaagg tcgcaactac attgtcacgt
    13141 aaaaaagaag ttgtatagga aatcggcaag cctggatgat ctgctgtaaa gcgactgcct
    13201 tcttgaatca agtcctctac catatccacc ttgcctgtta caactcgggc acccgaactt
    13261 gggtcgcccc ctaaaataac cgccttcact tctgtattgt ccaaaatctg cttccactct
    13321 gtctgaggag ctaccttgac tccttttatc aaagcttcaa aagcagcctc tacttcatca
    13381 ctcttactcg tggtttccaa cttgagatag acttggcgcc cataagcaac actcgaaata
    13441 tagaccaaag gacgctctgc agaaattcct ctctgtttta aatcctctac cgttacagta
    13501 tcttgaaaca catctcctgg atttttaaca gcgtctacgc tgactgtata ataaatctgc
    13561 ttaaaattaa caatctgaat ctgcttttcg cctgaatgga cagagttaaa atcaatatca
    13621 agagaattcc ctgtcttttc aaagtcagaa ccaaacttga ccttgagttg ttccatgctg
    13681 tgagccgtga ttttttcata ctgcattcta gctgggacat tattgacctg accataatct
    13741 tgatgccact tagccaacaa atcgtttacc gctccgcgaa cacttgaatt gctgggatct
    13801 tccacttgga gaaagctatc gctacttgcc aaaccaggca aatcaatact ataagtcatc
    13861 ggagcacgat cgaccgcaag aagagtggga ttattctcta acaaggtctc atccactacg
    13921 agaagtgctc caggatagag gcgactgtcg ttggtagctg ttacagaaat atcacttgta
    13981 tttgtcgaca agctccgctt ctttctttcg ataacaacaa actcatcggg tagctgatta
    14041 ccctctttga tgaaacgatt ttcaatactt tctccctgat gggtcaagag tttcttttta
    14101 tcgtaattca tagctagtat aaagtcattt actgctttat ttgccatctt ctacctccta
    14161 ataagttcct ggattgagtt gcataaactc agacttgttc agcgaaatca gccgtggttg
    14221 gactaagtga tccaaaattt cctcgtacaa ttcttctgag acattgcgtc gccgtctggc
    14281 tagataagaa gtcggaataa ccgtattatc caacataaat accttatcta agtcaatcaa
    14341 ggttggtctt gtaaaaggat tacgagctag atccggctct tctatcataa agttcttgac
    14401 caaacgtctg gtcaagagag ctggtttgaa ggtctgattt ttaaccaact ctttgttttt
    14461 agtcatgctg ttgtcaatac agatatacat atgattcttc acagccaaat cgctactaat
    14521 agtcggaaaa ggcaaataaa gagctacaac atctcctctc ttaatcaagc aagagcaccc
    14581 ccttttctcc taatgtaaca tagacaggat tgaccaagtc ttctgattga ctcagaattt
    14641 ccaaagtttg agtttggcgc gctgtcaatt tagtagcatc ttgtctcttc aatacaaaat
    14701 gcttgtcgcc aataaccttg acaatataat ccttctccaa agctgactgg taaatccaca
    14761 tcagatgttg tctgtcctga gaactcaaga gagaaggatt ttcaagcctc ccgatagtct
    14821 gataaaaatc aaaaacagga gctaactcct gccaatctga ttggctagtt gtcaaggcta
    14881 gaaaaagggc tttgcgagct gatacttctt ggttagcctt gagagttact ttcccctcca
    14941 agttttttag aaatcgggaa actccagaaa gcaaattttt ctctaactgc gagaaataaa
    15001 aacctttcgt tcccagacat aagtctttca tgtcgctttc tctagcaaat aagagctcaa
    15061 acatttgata gtaaaagaaa aatatctggc actgggtcgc gctcatcttt tccttatcgg
    15121 cttctttttt taaccagagc aagggcgaca ggtagctgga ttgagacatt tcctctacct
    15181 cctactcttt tttaactgga gcatctgcac tagctgccac ttcttttgac tggatacttt
    15241 cccactggtt aatctcctct gagataagac cttcgcatgt cttgacaaat agggcaaaag
    15301 ccttggtctt tcctgcatat ttctccgttt ggcattgata gaggaatttt tctttctcca
    15361 ggagttgcgc agttttttgg taagaaatcc aattttcctt tgcattatac aaattgataa
    15421 tcccctcaca cagcaagccg agactggata aggcaaccga aatcaaacgg tagcgatcac
    15481 ctggcatagg aatagcacaa aagacagcta tgaggaaacc tgccacgatt tctgttattt
    15541 ttaatacctt atagcgtcta cgatgttgaa cgcttttctt taaaaaatga gctatctgta
    15601 cgtctaatcg ctctgtcagg tacatttctt ctggcgtcat attcgtaact cctttcattt
    15661 actttgataa ccatagtata acatatttga atatcaaagt atagtaaaaa ctggactata
    15721 acttattaga cttcctgcga aacaaaatat ggtatagtag ttctatgaat tatgaagcaa
    15781 gtaaacaact aactgatgca cgatttaagc gtcttgttag tgttcagcgt accacttttg
    15841 aagagatgtt agctgtatta aaaacagctt atcaaaaaag tcggacaagc tggtaaaatc
    15901 ttggctgaca gtaattatca agggctcatg aagatatatc ctcaagcaca aactccacgt
    15961 aaatccagca aactcaagcc actaacagtt gaagataaag cctgtaacca tgcgctatcc
    16021 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgatttca
    16081 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggtatt
    16141 atcaatcatg aactaggatt ttggtttcgc aggaagtcta ttatatatgc acagacatat
    16201 gaaatctttt tgatacagcc ttctttttac atagagagac agcagttatg atatatttat
    16261 ccattcacaa catcttctgt accaatacta caatattggt acattttgat tacacaatcg
    16321 tttcaaaaaa tatgatggaa gcctgagtat tttatagtaa ttcccttcct atcaactaga
    16381 tcaaccagtt gagtgttgaa acaccctagc ctcagcaaag gactatgatc agcatttcct
    16441 agccaatagg acgctccaaa tcacctattc taagtccatc actagcttgg tcagtaagat
    16501 acagactgca aggcgagact gacgtggttg atgagatttt tgaagagtat tacaatcata
    16561 gattcaccag tagacttgac cgcagattta actatagtag attgaaacta gaatagtaca
    16621 cttctgcttc taaaacattg ttagaaatcg atttgactgt cctgatcaat ttgtcctgtt
    16681 ctcgtttcat tttactatac taaaaaacca cctgaaacaa atcaagtgac tagaggataa
    16741 aaacaaagac tgggcaaaaa gttttgaaaa gcaaaaaacg cataatatca ggtcttgaaa
    16801 aaccttgata ctatacgttt gattgtggga agattgactt cattttctcc tgaaattgaa
    16861 gttttttcta gtttccagat atagacgaat tcaatatagc catctcgcta caattatttt
    16921 aaccatcgaa aaaatttttc agaatttcaa aaatttcatt ttgatctaat gaaatgtcat
    16981 cattttgaag aatagttgac ttcacaagct ctaattctat cacaaagcgg tgcatgtctg
    17041 cttttaccgc actacttgat gttatagccg ataacttttc atctcctagc aaggaagctg
    17101 tcaaacgggt aaggtcattc aaatgctttt taatggactt acttaaacct tgagcacctg
    17161 tagcagagcg ttccctcata tccagccaag ctttagcctt aaaaataatt aatactcaat
    17221 gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc tcaaaacact gttttgaggt
    17281 tgtagatgga agctgacgtg gtttgaagag attttc
//
LOCUS       DAVKAO010000037        16534 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKAO010000037 DAVKAO010000000
VERSION     DAVKAO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.037"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(822..2072)
                     /gene="ilvA"
                     /locus_tag="VWX16_001305"
     CDS             complement(822..2072)
                     /gene="ilvA"
                     /locus_tag="VWX16_001305"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9158648.1"
                     /translation="MLSSKDIIQAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(2122..2478)
                     /locus_tag="VWX16_001306"
     CDS             complement(2122..2478)
                     /locus_tag="VWX16_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9158649.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(2716..2952)
                     /locus_tag="VWX16_001307"
     CDS             complement(2716..2952)
                     /locus_tag="VWX16_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158650.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(3059..4081)
                     /gene="ilvC"
                     /locus_tag="VWX16_001308"
     CDS             complement(3059..4081)
                     /gene="ilvC"
                     /locus_tag="VWX16_001308"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9158651.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(4147..4623)
                     /gene="ilvN"
                     /locus_tag="VWX16_001309"
     CDS             complement(4147..4623)
                     /gene="ilvN"
                     /locus_tag="VWX16_001309"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9158652.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(4616..6316)
                     /locus_tag="VWX16_001310"
     CDS             complement(4616..6316)
                     /locus_tag="VWX16_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9158653.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(6517..8184)
                     /locus_tag="VWX16_001311"
     CDS             complement(6517..8184)
                     /locus_tag="VWX16_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9158654.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(8187..8552)
                     /locus_tag="VWX16_001312"
     CDS             complement(8187..8552)
                     /locus_tag="VWX16_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9158655.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(8709..8897)
                     /locus_tag="VWX16_001313"
     CDS             complement(8709..8897)
                     /locus_tag="VWX16_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9158656.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(9013..9189)
                     /locus_tag="VWX16_001314"
     CDS             complement(9013..9189)
                     /locus_tag="VWX16_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9158657.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            complement(9210..9482)
                     /locus_tag="VWX16_001315"
     CDS             complement(9210..9482)
                     /locus_tag="VWX16_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEU9158658.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTLKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIDSSILKGYRLPFEGLSYAAFLGILFQSM"
     gene            complement(9737..11281)
                     /locus_tag="VWX16_001316"
     CDS             complement(9737..11281)
                     /locus_tag="VWX16_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9158659.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            11494..11796
                     /gene="gatC"
                     /locus_tag="VWX16_001317"
     CDS             11494..11796
                     /gene="gatC"
                     /locus_tag="VWX16_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9158660.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            11796..13262
                     /gene="gatA"
                     /locus_tag="VWX16_001318"
     CDS             11796..13262
                     /gene="gatA"
                     /locus_tag="VWX16_001318"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9158661.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            13262..14704
                     /gene="gatB"
                     /locus_tag="VWX16_001319"
     CDS             13262..14704
                     /gene="gatB"
                     /locus_tag="VWX16_001319"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9158662.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LDGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            14833..15393
                     /gene="efp"
                     /locus_tag="VWX16_001320"
     CDS             14833..15393
                     /gene="efp"
                     /locus_tag="VWX16_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9158663.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            15415..15804
                     /locus_tag="VWX16_001321"
     CDS             15415..15804
                     /locus_tag="VWX16_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9158664.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            15797..16219
                     /gene="nusB"
                     /locus_tag="VWX16_001322"
     CDS             15797..16219
                     /gene="nusB"
                     /locus_tag="VWX16_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9158665.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(16254..>16534)
                     /locus_tag="VWX16_001323"
     CDS             complement(16254..>16534)
                     /locus_tag="VWX16_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9158666.1"
                     /translation="EPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSN
                     AKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         4816 a         3758 c         3019 g         4941 t
ORIGIN      
        1 ctacttttgt caataaaaaa aacatatatt caacgaaaat aattagttca gaattttctt
       61 acttcctttt taaatccgat aatatatagg tttttctagc ctctacacca taaatatttg
      121 ctaataaaaa tactgtatta ttaccctctt aaggtgcgaa attattcaag ttcttaaaaa
      181 atgcttctct atttccgtaa gatagcttat ttcttcgatt taagaactga agatgatagt
      241 tctaaccttt agaaatccaa gccttcacta gctcaaaaac tcctatcttt cttcctcaat
      301 aaatcatctg agagataggt attccttatg tccttcaaat tcatcactcc tttggatatt
      361 gaggtgctag cctagaagca gacacccgat tatctaaacc atccttaata gagatgacaa
      421 gacagataag ctcagttaat tacttatata tctttttaat atggtatatt tgtcaacaaa
      481 aagaaatcaa atcatacctt atactcaatg aaaatcaaag agcaaactag gaagctagcc
      541 gcaagttgct caaaacactg ttttgaggtt gcagatagag ctaacgtggt ttgaagagat
      601 tttcgaagag tattaaagac agtgtgacta tgcttgtcaa tagataggaa atcaaaatga
      661 aggatggaaa tctgagccaa acatcataag tgctataatg aacagaagta agaattgcac
      721 gataagtttt ctaaaaccat aggaatcgtc ccctttcttc atcttcatta tagcacatat
      781 acatcagttg tacaaataat atgatatttt tttattagtc ctcagacaag catattataa
      841 agcgtttcat taccatttaa gttaatataa gctggatcaa aaccttccat tctacgaatc
      901 aaacctgcat aatcatgctt atctgctaaa gcgatcccaa ttaatactgg gcctgttccc
      961 ttgctagctc gtttgatata ctcaaaacgt gtgatatcat catttggccc caggatatca
     1021 tttacaaact cacgcaaagc tcctggacgt tgtgggaaat tgaccacaaa gtaatgtttg
     1081 ataccatcat aaatcaaggc acgctcttcc atttctggca tacggttgat atcattattt
     1141 cctccagaaa tgatacaaca aatggttttc cccttaatat attcagctaa aacctctaaa
     1201 gaggcgatac tagccgctcc agcaggttct gcgactatcc cttgcttaga gtaaaggtca
     1261 atcaaggttt cagaaatcaa tccctcatcg acacctacca aagttttaat atgttgacga
     1321 gttgcttcat aggtcaactg acctaccttt tgcacagcaa tcccatcagc aaatttatca
     1381 atttccttga gttttactgg acctccagcc tcaaaggcag ctttcatgga acgcgctcca
     1441 ttcgcctcta ctccgataac ctcaatctct ggacttgttt ccttgatata ggtagaaacc
     1501 ccggcaatga gaccgccacc accaacagga accaagacag catcaaaatc aatcgattct
     1561 tttcgagctt cttctaaaat ctcataagca actgttcctt gacctgcttg aacatgagca
     1621 tcatcaaaag gatcaataaa ggtacgattt tcagagactg taaattcttg agctgctttg
     1681 gctgaggcat caaaggtatc tccaactagt ttaatagtta caaaatcccc accaaaaaag
     1741 cgaacctgac caatcttttg ttgtggcgta gtaatgggca taaagatagt agcaggaatt
     1801 ttcatttcat tacaagtata ggctactccc tgcgcatgat ttcccgcaga agcgcagact
     1861 accccacgtt cacgttcttc cttgctgagc tgggaaatgg cataataggc accacgaatt
     1921 ttaaaggagc gaacacgctg ggcattttct tttttcaaat aaatcttagc accatacttc
     1981 tccgataaat aatgatcgta atccagtgga gtattcacaa ccacaccgtt caagaccttg
     2041 tgagcctgga ttatatcttt tgaacttaac atataaaacc tctttttcta taaaaaacag
     2101 tcactaatga ctgtttttta gttattcatc tccgaaaact tcagtaacac tttcatggta
     2161 tttatgagcc atacgaatcg aaaactcttt aggaagttca taacgatttg tggaaatcca
     2221 atcatataac cattctacca aactttcttc actatcatta cgatggaaga taaaatagtt
     2281 taaaatttct ttatattcta catcattcaa atccaacgtg tctacaaatt gactacgagg
     2341 aacaaaatta gaatctaatt ttcttagttg atctgtcaat tctcccaatt gtaaatccaa
     2401 ctgaaaaaat agtttttgca gttgcctatc agtttcatca catcctgtct tgatagaaaa
     2461 caaattggta atattcatat gatttcaacc cctaaataag tacatctaat atcccacgaa
     2521 tagctgaagc taaagttttc gcagtccagt catttaaacc cgattctttc aatccagatg
     2581 caataatttg ctctatttga tataaaccgc catctatcgt ctcatttatt ctgcgaagag
     2641 gaccttcaat ataggataaa gcttctccga atctaacagc tgatttaggt cctaatgatt
     2701 taccaactat atttgttaat gttttttatt tctcaaaatc catttcgcta tataacgagc
     2761 agatttagtc gcgccagctc tctcaaacgg agaatgaatt tcaagacctt cttctctagc
     2821 tatttgtgcc aatttgaaga attgttcaaa ggtcatatgc aacctaccat cttcaaaatt
     2881 agcatcatca taaatgttaa ttacagcatt ttcatcatac gaccaatctg ctttattttg
     2941 gtctgctagc actattaccg acggtgtaat tacattatac aactagaacc aataataacg
     3001 atgcaatttt tctcataatc tttttcataa aatcatctcc agcgctatat atttctaatt
     3061 agttatagat tttgaatgca tcatcgtcgt ttttaccaac gaatggcatt gctttacgca
     3121 attctgcacc aactttttca atttcaaggt tagctgcttg ttcacggtaa gcagtcaatt
     3181 ttggacgtcc agctttatag tcatttacaa agtcatttgc aaatttacca ttttggatgt
     3241 ctgccaagac agccttcata ttttctttaa cttgttcagt gattacacgt ggacctgata
     3301 catagtcacc gtattcagca gtgtttgaaa tagattgacg cattttcttg aatccacctt
     3361 cgtagatcaa gtcaacgatc aatttcattt cgtgaagaac ttcaaagtaa gccaattctg
     3421 gagcgtaacc tgcttctgtc aagacttcga aacctgcttc gataagggca gtcaaaccac
     3481 cacaaagtac agcttgttca ccaaacaaat cttcttcagt ttcttctttg taagttgttt
     3541 caagaagacc tacacgagcc gctccaacac ctttacacca gtccatagca atgtttttag
     3601 catttcctgt tgcatcttgg tatactgcat aaagagctgg aacaccaaat ccttcttcgt
     3661 aagtacgacg taccaagtgt cctggtcctt taggagcaca catgaagaca tctacatccg
     3721 caggaacttt gataaattca aagtggatgt tgaaaccatg ggcaaatcca actgcgtttc
     3781 cagcttccaa gtttggagcg atttctgctt cgtacaattc ttgttgaatt tcgtctggcg
     3841 ccaagatcat gataacatca gccaacttag tagcttctgc tactgtgtaa gtatcaaatc
     3901 catcttcttt tgctttatca aaagatttac ctggacgtac acctataata acgtcacgac
     3961 ctgaatcacg caagttttga gcatgcgcat gcccttgtga accataaccg ataacggcga
     4021 tttttttacc gtcaagtgct gctactttaa catctttttc atattccatt tgaactgtca
     4081 tagcttttct ctcttttcta ttatttattg ccttttaggc tggtttaata aatttaagtt
     4141 ggatttttaa tcgcgggtaa atccagttgc acccgttcga gcaatattgc gaataccgta
     4201 tgggcgaatg actctcaata gggcttcgct cttttctgca tttcccgtca tctgaatggt
     4261 aatcgagctt ggcgctacgt ctaccactgt tgcacggaaa ggttgaataa tcgctaaaat
     4321 ctcagctctc ttctcagctg gcgctgacat cttaaccaaa atcacctcgc gctccaaatg
     4381 aggcttgtct gtaatatctc gaatgcgaat cacatcaatc tgacgattga gctgtttgat
     4441 gatttgctcc acttcatcat gagaagcaac atcaataata atagtgatac gcgatacatt
     4501 cggatcttct gttgctccaa cagagatgct ttcaatatta acctgacgac gagataggac
     4561 acctgtaaag cgattgagga ctcctgaacg attttgtagt tttgctgtta acattctacg
     4621 catggaactg cacccccaac atctcatgat tactcttacc agccggtacc attggtaaca
     4681 cctgttcctt acgagaaata tctacctcaa ttagcatagg aacatcctca gtgatgactt
     4741 caaggtcttg agccaaggtc tcaggattgt caaacttata gtttttaata ccataagcct
     4801 gcgccatcaa ttggaaatca ggaagggtat caaagaccga ctctgatgtt ctgccttcat
     4861 agaaggattc ctgccactgg cgaaccattc cgagtgaatg attgttcagc ataaccacct
     4921 tgattggcac cttgtaaata ttcaaaatag ccaactcctg gttagtcatt tggaaaccac
     4981 catccccaac aaacaagact acttccttat ctgggttagc aattttagca ccgattgctg
     5041 ctggaattcc aaagcccatt gttcccaaac cacctgaagt cactaactga cgttcatttt
     5101 ggtagggata atactgagct gtccacattt ggtgttgacc aacgtctgtt accacaatgg
     5161 catctccatt cgtcaattca ccaattcgtt caataactgc ttgcggttga accacacgct
     5221 ctttcttgtc ataagaacga acacgattct tgtctttagt gactttctca atccactttt
     5281 cagtattgtt gtgaactgtt ggttctgcta gcaacatttg caaggccttc ttagcatctc
     5341 caactacagg aatgtctgca ctgataatct tgccaatctc agctgggtca atatcaatgt
     5401 gggcaacctt agcattctta gcgaaagtct taggattccc cgtcaaacgg tcatcgaaac
     5461 gagaaccaat actaatcata aagtccgctt ccgtcatggc aatatttgct gcgaatgacc
     5521 cgtgcatgcc tcccattcca agaaagagtg ggtgactcgt tgcaatcgtt ccttgtccca
     5581 aaagactggt taccactgga atttgatagc gttctgcaaa ttcatttagt tccgtagcag
     5641 cctcagcata actaattcca ccaccagcta acaagactgg ctttttagcc ttggacaatt
     5701 gcttcaagat tttcttgatt tgcatatcat tcggctcaag agtcggctga taacttggta
     5761 aattcacttc tggtgaataa atgaagtctg tttctaaagc agatatgtct tttggtaggt
     5821 caattacaac tggccctgga cggcctgtag ttgcgatatg gacagcttcc gtaatgatac
     5881 gcggaatatc agctgtctca cgaacttggt aattgtactt agtgattggc atggtaattc
     5941 ccacgatgtc tgcctcctga aaggcatcct tcccaatccc tgctcgcgcc acctgacctg
     6001 taaagaccaa aaggggaacg ctatcgctca tggcatccgc aatccctgta atggcatttg
     6061 ttgctcctgg tccactagta acgacggcaa cacccaactt tccagttgat ttggcataac
     6121 cttcagcttc atgcaaacaa ccttgctcat ggcgccctag aatgtggcga atgcctttaa
     6181 aattatatat cgcatcataa aaaggcaaaa ccgcaccacc aggataacca aagatggtat
     6241 caactcctaa atcacgaagt gtttccaaaa ctaggtccga ccccgtctta ggagattcta
     6301 aactgatttt ctccattgtt ccccgttctt ttctcttaaa aataacttgt tactatcata
     6361 ccattttttc aagatttttc aagagaaaag aagaaatttt ctgaattttc tattttaacg
     6421 tttatttatg aatgttattt ttgtttttat taaaaagata ccgatttcat ttactaaaaa
     6481 agaaaaaaga actgatttct cagtccttca ttaatcttat tccacactaa ataggtatgg
     6541 gtaaacaggt tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt cttctacgat
     6601 ttcttgagcg atttcattgg caagttcttc gcttccgtct tcaccgacat agaaggttac
     6661 gatttcactg tcttcatcca acatatgttt caaggtttca gtcaatgttt ggtgcatatc
     6721 agggtttgac acaagaattt ttccatccac catacctaga ttatcgtttt catggatttc
     6781 taagccatcg atcgttgtat cacgcacggc tgttgtgacg cttccgctaa cgacatcgct
     6841 aagagcagct gtcatacgct cttgattttc ttcaatggac ttgcttggat caaaggcaag
     6901 aagacttgtc ataccttgag gaagagtgcg agcctctacc actactgctg gttgctccaa
     6961 aacttctgcc gcagattgag ctgccatgaa gatgttcttg ttgtttggca agaagatgat
     7021 gttacgggca ttgacctgtt caacagcctt gataaagtct tctgttgaag ggttcatggt
     7081 ttgaccgcct tcgataacat aatccacgcc ttgagaacag aagatatctg ctagaccttt
     7141 accagccacc acagcaatca aagcatactc tttttcttca gccgacttga taacttgagt
     7201 agcttctttc tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt ttaccttgac
     7261 caagctacca tatttgagac cttcttgcat aacaagtcct ggatcttctg tatgaacatg
     7321 gactttgaca atttcatcat cgttaacaac gaggagagaa tccccaagct catccaagta
     7381 gttacggaat tcatcgtagt caaaatcttt agcataggtt ggaccttgct taagagctac
     7441 catgatttca gtacagtaac caaacgtgat gtcctcagtc gctacgtgac cagctacaga
     7501 cttatgatgc tctacattga tcatctcact catgttggca ggagtcgcta caaagtcctc
     7561 agatgcaata tattcgccag taagggctga aaggaaacct tcgtagatga agaccaatcc
     7621 ttgaccacct gagtccacaa cgccaacttc tttcaatact ggaagcatgt ctggtgtttt
     7681 agctagagct gttttagcac cttccaaggc tgcgcgcatg acttcaacag cgtcatctgt
     7741 ttgctcagct tttttcttag caccgatagc agctccacga gaaactgtta aaatcgttcc
     7801 ttcaacaggt ttcatcactg ccttataggc aacttccaca cctgattgga aggccagagc
     7861 caagtcttga cctgttaact cgtctttatc cttgatagct tgggaaaatc cacggaaaag
     7921 ctgagacgta atcactcctg agttcccacg cgcacccatc aaaagccctt tggcaagaat
     7981 gctcgctact tctccaactg tagaagctgg cttgtctgca acttctttag caccattttc
     8041 aatggtcatt cccatatttg tcccagtatc tccatctgga actggaaaga cgtttaatga
     8101 attgacatat tcagcttgct tattcaagcg agttgatgca gcctgcacca tttcttgaaa
     8161 taagctagta gtaatttttg acacggttat tctcctacaa ctttgatatt ttgaatgtag
     8221 acatttacag tctgagcagt aattccaagc tggttttcca agctaaaggc aacacgctct
     8281 tgaatgtttt ttgacacttc actaatcttt gttccgtagc ttaacacggt atatacatca
     8341 actgcaatac tgccatcttc ggctgccttt acgacgacac ctttagaata attttcctta
     8401 cctagcaggg cttggaaatt atctttgagg gcatttttac tagccatacc gaccacacca
     8461 aaaatctcag ttgctgctcc gcctacgacg gttgcaatca cttcatctgt tagttcgatt
     8521 tgaccatctt ttgtattaat ttttacagtc atccttttta cctcaactag ttgatactct
     8581 attttatcat atttcagccc aagtgtaaaa gcagaatgat gtatcagcgg atatttactc
     8641 tatttttcaa acgatgttat acccacaata aaagaaaaaa gaccctaagg tctcctttgc
     8701 ttttattatt aaacgcgttc aactttacct gatttcaaag cacgagctga agcccaaact
     8761 tttttaggtt taccatcgat aagaacagta actttttgaa ggtttggttt tacggcacgt
     8821 tttgtttggt tcatcgcgtg tgaacggttg tttcctgata cagtcttacg acctgtaaag
     8881 taacatactt tagccattgt gttttcctcc tattagatct aatatagcgg atgtgctagc
     8941 accacatacc gtactatgtt atcacacttt cttgtttttt gcaagggaat tggaagattt
     9001 tttatttgtg tcttaaatca ggtcttgcgt gacattttct gctctccaca tgccattgtt
     9061 gattaacaga acaccagaat taaaattatg tgtataaaaa tcatctctaa ctgcagctaa
     9121 gggtatagcc gtcaagtcca aatcccacag ctcatctatc gattttctta caacaatatc
     9181 taaatccaaa tacagtacac gagactcgct tacatacttt ggaataaaat acctaaaaaa
     9241 gccgcatatg aaagtccctc aaaggggaga cgataacctt tcagaatact actgtcaatc
     9301 tcactattca aagtctctag tcttttttcc atcaattgga accattctcg cggaaggtca
     9361 tcattaaaaa cataaaattt aagattataa tgatgaacac aaagagattt tagtgttgtt
     9421 tcaactttat ccatataagc attatctgca cctaagacaa tcgctttttt ctcttctttc
     9481 actctttatc ttatttcttt ttattcccat catatgtttc tacgtaacca ttattttcgc
     9541 ctattcgttc gtaaaaccat accagtggag attttagatg aagtcccatt acggtttaca
     9601 atttttacat tacgacacgg agttttacaa atcgatttca tttgccaaac gtagttagta
     9661 aggcagttag ctagttcgcc aaatagcgac tagcgtccaa caatttggaa ctttagttcc
     9721 aattgttggt actgagtcac atcttctcct ctaactctac gtctggatac ttgtccgcaa
     9781 accagcggag ggcaaagtca ttttcaaaga gaaagactgg ttggtcaaaa cggtctttgg
     9841 ctaagatatt gcgacttgac gacatccgtt catccaagtc ctcaggcttg atccaacgaa
     9901 cggtcttttt acccattggg ctcatgacca cttccgcatt gtattcgcct tccatacggt
     9961 gtttaaagac ttcaaactgg agttgaccaa cagctcccag catgtactca cctgtttggt
    10021 aattcttata aagctgaacg gctccttctt gcaccaattg ctcaatcccc ttgtggaagg
    10081 atttttgctt cataacattc ttagcagaaa ctttcatgaa aatttcagga gtaaaggttg
    10141 gcagtggttc aaattcaaac ttgttttttc caaccgtcaa ggtatcccca acctgataag
    10201 taccggtatc gtaaaccccg ataatatcac ctgctacggc attggtcaca ttctcacgac
    10261 tctccgccat aaactgggta acattagata gtttggcacc cttaccagta cgagggagat
    10321 tgacactcat gccacgctca aattcgcctg atacgatacg gacaaaggca atacggtcac
    10381 ggtgacgagg atccatgttg gcttggattt taaagacaaa gcctgagaaa tccttgtcat
    10441 aaggatccac aatttcaccg tctgttttct tgtgaccatg tggttctgga gcaaacttga
    10501 ggaagatttc aaggaaggtc tgcacaccaa agtttgtcag ggctgaaccg aaaaagacag
    10561 gcgtcaattc tccagccaga atagcttcct ctgaaaactc attcccagct tcatttaaaa
    10621 gctcaatgtc atccttgact tgctcgtaga aaggattgct accaaaaagt ttgtctccat
    10681 cttctaggct agcaaaacgc tcatcccctt tgtaaagctc taaacgttgg ttatagaggt
    10741 catacaagcc ctcaaaggct ttccccatcc cgataggcca gttcataggg tagctagcaa
    10801 tgcccaagat ttcttccaat tcttgcaaga gatccaaagg ctcacgaccg tcacgatcca
    10861 acttgttcat aaaggtaaag actggaatgc cacgatgttt cacaacctca aacaatttct
    10921 ttgtttgagc ctcgatcccc ttggcagagt ccacgaccat gaccgcagca tccaccgcca
    10981 tcaaggtacg ataggtatct tctgagaagt cctcgtgccc tggcgtgtct aagatattca
    11041 cgcgcttgcc gtcgtagtca aattgcataa cagatgaagt aacagaaatc ccacgttgct
    11101 tctcgatatc catccagtca gatttagcaa aagtccctgt tttcttccct tttaccgtac
    11161 cagcctcacg aatctcaccc ccaaagtaga gtaactgctc agtgatggtt gttttccccg
    11221 cgtccgggtg ggagataatg gcaaaggtac gacgtttctt aatttcttct tgaatattca
    11281 taagttctct ttctttgatt ctctattttt cttgtttcaa tagctgagaa tgatttttac
    11341 attggatttt accattcctt tcgacactcc attatatcgg attttagcat ttttttcaat
    11401 ttctatttct tttcacttcc ccctccctta tttataggaa aatatggtaa aatagaacag
    11461 actaaaaatc atcatttcac gaaaggatgc aagatgaaaa ttacgcaaga agaggtaaca
    11521 cacgttgcca atctttcaaa attaagattc tctgaaaaag aaactgctgc ctttgcgacc
    11581 accttgtcta agattgttga catggttgaa ttgctgggcg aagttgacac aactggtgtc
    11641 gcacctacta cgactatggc tgaccgcaag actgtactcc gccctgatgt ggccgaagaa
    11701 ggaacagacc gtgatcgctt gtttaaaaac gtacctgaac aagacaacta ctatatcaag
    11761 gtgccagcta tcctagacga tggaggagat gcctaatgac ttttaacaat aaaactattg
    11821 aagagttgca caatctcctt gtctctaagg aaatttctgc aacagaattg acccaagcaa
    11881 cacttgaaaa tatcaagtct cgtgaggaag ccctcaattc atttgtcacc atcgctgagg
    11941 agcaagctct tgttcaagct aaagccattg atgaagctgg aattgatgct gacaatgtcc
    12001 tttcaggaat tccacttgct gttaaggata acatctctac agacggtatt ctcacaactg
    12061 ctgcctcaaa aatgctctac aactatgagc caatctttga tgcgacagct gttgccaatg
    12121 caaaaaccaa aggcatgatt gtcgttggaa agaccaacat ggacgaattt gctatgggtg
    12181 gttcaggtga aacttcacac tacggagcaa ctaaaaacgc ttgggaccac agcaaggttc
    12241 ctggtgggtc atcaagtggt tctgccgcag ctgtagcctc aggacaagtt cgcttgtcac
    12301 ttggttctga tactggtggt tccatccgcc aacctgctgc cttcaacgga atcgttggtc
    12361 tcaaaccaac ctacggaaca gtttcacgtt tcggtctcat tgcctttggt agctcattag
    12421 accagattgg accttttgct cctactgtta aggaaaatgc cctcttgctc aacgctattg
    12481 ccagcgaaga tgctaaagac tctacttctg ctcctgtccg catcgccgac tttacttcaa
    12541 aaatcggcca agacatcaag ggtatgaaaa tcgctttgcc taaggaatac ctcggtgaag
    12601 gaattaaccc agaggttaag gaaaccattc taaatgccgc taaacacttt gaaaaattgg
    12661 gtgctattgt cgaagaagtc agccttcctc actctaaata cggagttgcc gtatactaca
    12721 tcatcgcttc atcagaagct tcatcaaact tgcaacgctt cgacggtatc cgttacggct
    12781 atcgcgcaga agatgcaacc aaccttgatg aaatctatgt aaacagccga agccaaggtt
    12841 ttggtgaaga agtgaagcgc cgtatcatgc tgggtacttt cagtctttca tcaggttact
    12901 acgatgccta ctataagaag gctggacagg tccgtacact tatcattcaa gatttcgaaa
    12961 aagtcttcgc ggattacgat ttgattttgg gtccaactgc tccaagtgtt gcctatgact
    13021 tggattctct caaccatgac ccagttgcca tgtacttagc cgacctattg accatacctg
    13081 taaacttggc aggactgcct ggaatttcga ttcctgctgg attctctcaa ggtctacctg
    13141 tcggactcca attgattggt cccaagtact ctgaggaaac catttaccaa gctgctgctg
    13201 cttttgaagc aacaacagac taccacaaac aacaacccgt gatttttgga ggtgacaact
    13261 aatgaacttt gaaacagtca tcggacttga agtccacgta gagctcaaca ccaattcaaa
    13321 aatcttctca cctacttctg cccactttgg aaatgaccaa aatgccaaca ctaacgtgat
    13381 tgactggtct ttcccaggag ttctaccagt tctcaataaa ggggttgttg atgccggtat
    13441 caaggctgct cttgccctca acatggacat ccacaaaaag atgcactttg accgcaagaa
    13501 ctacttctat cctgataacc ccaaagccta ccaaatttct caatttgatg aaccaatcgg
    13561 atataatggc tggattgaag tcaaactaga agacggtacg accaagaaaa tcggtatcga
    13621 acgtgcccac ctagaggaag acgctggtaa aaacacccat ggtacagatg gctactctta
    13681 tgttgacctc aaccgccaag gggttccctt gattgagatt gtatctgagg cagatatgcg
    13741 ttcgccagag gaggcctatg cttatctgac agccctcaag gaagttatcc agtacgctgg
    13801 catttctgac gttaagatgg aagaaggttc tatgcgtgtg gatgccaaca tctcccttcg
    13861 cccttatggt caagaaaaat tcggtaccaa gactgaattg aagaacctca actccttctc
    13921 aaacgttcgt aaaggtcttg aatacgaagt ccaacgccag gctgaaattc ttcgctcagg
    13981 tggtcaaatc cgccaagaaa cacgccgtta cgatgaagcg aataaagcaa ccattctcat
    14041 gcgtgtcaag gaaggggctg cagactaccg ctacttccca gaaccagacc tacccctctt
    14101 tgaaatttct gacgagtgga ttgaggaaat gcggactgag ttgccagagt ttccaaaaga
    14161 acgtcgtgcg cgttatgtat ctgaccttgg tttatcagac tacgatgcta gtcagttgac
    14221 tgctaataaa gtcacttctg atttctttga aaaagctgtt gctctagatg gtgatgccaa
    14281 acaagtctct aactggctcc aaggggaagt cgctcagttc ttgaatgctg aaggtaaaac
    14341 actggaacaa atcgaattga caccagaaaa cttggttgaa atgattgcca tcatcgaaga
    14401 cggtactatt tcatctaaga ttgccaagaa agtctttgtc catctagcta aaaatggcgg
    14461 tggcgcgcgt gaatacgtgg aaaaagcagg tatggttcaa atttcagatc cagctatctt
    14521 gatcccaatc atccaccaag tctttgccga taacgaagct gctgttgccg acttcaagtc
    14581 aggcaaacgt aacgccgaca aggcctttac aggattcctt atgaaggcaa ccaaaggcca
    14641 agccaaccca caagttgccc ttaaactact tgcacaggaa ttggcgaagt tgaaagaaaa
    14701 ctagacagaa caaaaccagc cctaaggttg gttttttctt ctctaccaac tcccaataac
    14761 tattttggct ttatttccag agtattttat ggtaaaatga agagtaataa tatttattaa
    14821 agaggtaaaa acatgattga agcaagtaaa ttaaaagctg gtatgacctt tgaaacagct
    14881 gacggcaaat tgattcgcgt tttggaagct agtcaccaca aaccaggtaa aggaaacacg
    14941 atcatgcgta tgaaattacg tgatgtccgt actggttcta catttgacac aagctaccgt
    15001 ccagaggaaa aatttgaaca agctattatc gagactgtcc cagctcaata cttgtacaaa
    15061 atggatgaca cagcatactt catgaataca gaaacttacg accagtacga aatccctgta
    15121 gtcaatgttg aaaacgaatt gctttacatc cttgaaaact ctgatgtgaa aatccaattc
    15181 tacggaactg aagtgatcgg tgtcaccgtt cctactactg ttgagttgac agttgctgaa
    15241 actcaaccat ctatcaaagg tgctactgtt acaggttctg gcaaaccagc aacgatggaa
    15301 actggacttg tcgtaaacgt tccagacttc atcgaagcag gacaaaaact cgttatcaac
    15361 actgcagaag gaacttacgt ttctcgtgcc taatctctag aaagaggtca ttctatggga
    15421 attgaagaac aacttggcga aatcgttatc gccccacgtg tacttgaaaa aatcattgct
    15481 attgctactg caaaggtaga gggtgttcac tctttttcaa acagatcagt gtctgatacc
    15541 ctttcaaaac tttcactcgg ccgtggcatt tatcttaaaa acgtggacga agaactcaca
    15601 gcagatatct atctctacct tgagtacgga gtaaaagttc ctaaggtagc ggttgctatc
    15661 cagaaagctg tcaaagatgc cgtccgtaat atggctgatg tagaactcgc tgctatcaat
    15721 attcacgttg caggtatcgt cccagataaa acaccaaaac cagaattgaa agatctattt
    15781 gacgaggact tcctcaatga ctagtccact attagaatct agacgccaac tccgtaaatg
    15841 cgcttttcaa gctctcatga gccttgagtt cggtacggat gtcgaaactg cttgtcgttt
    15901 cgcctatact catgatcgtg aagatacgga tgtacaactt ccagcctttt tgatagacct
    15961 cgtttctggt gttcaagcta aaaaggaaga actagataag caaatcactc agcatttaaa
    16021 agcaggttgg accattgaac gcttaacgct cgtggagaga aacctccttc gtttgggagt
    16081 ctttgaaatc acttcatttg acactcctca gctggttgct gttaatgaag ctatcgagct
    16141 tgcaaaggac ttctccgatc aaaaatctgc ccgttttatc aatggactgc tcagccagtt
    16201 tgtaacagaa gaacaataag gctctttgtc aactgtagtg ggttgaaaaa aagctaagct
    16261 cgagaaagga caaatttcgt cctttctttt ttgatgttca gagcgatgaa aatccgtttt
    16321 ttgaagtttt caaagttccg aaaaccaaag gcattgcgct tgataagttt gatgagatta
    16381 ttggtcgctt ccagtttggc attagaatag tgtagttgaa gggcgttgac aatcttttct
    16441 ttatctttga ggaaggtttt aaagacagtc tgaaaaatag gatgaacctg cttaagattg
    16501 tcctcaataa gtccgaaaaa tttctctggt tcct
//
LOCUS       DAVKAO010000038        16167 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKAO010000038 DAVKAO010000000
VERSION     DAVKAO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16167
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.038"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            121..2286
                     /locus_tag="VWX16_001324"
     CDS             121..2286
                     /locus_tag="VWX16_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9158667.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2286..3026
                     /locus_tag="VWX16_001325"
     CDS             2286..3026
                     /locus_tag="VWX16_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9158668.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3175..4662
                     /locus_tag="VWX16_001326"
     CDS             3175..4662
                     /locus_tag="VWX16_001326"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9158669.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDLEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(4706..5995)
                     /gene="ftsY"
                     /locus_tag="VWX16_001327"
     CDS             complement(4706..5995)
                     /gene="ftsY"
                     /locus_tag="VWX16_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9158670.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTIEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5999..6817)
                     /locus_tag="VWX16_001328"
     CDS             complement(5999..6817)
                     /locus_tag="VWX16_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9158671.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYADVARLYEKTE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6817..7611)
                     /locus_tag="VWX16_001329"
     CDS             complement(6817..7611)
                     /locus_tag="VWX16_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9158672.1"
                     /translation="MTIKLVATDMDGTFLDENGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            complement(7608..11147)
                     /gene="smc"
                     /locus_tag="VWX16_001330"
     CDS             complement(7608..11147)
                     /gene="smc"
                     /locus_tag="VWX16_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9158673.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDIFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQTQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEVALKTLQDEMARLTESLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDRYEEVHNRLDFLNSQRDDILSAKNLLLETITKMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11138..11836)
                     /locus_tag="VWX16_001331"
     CDS             complement(11138..11836)
                     /locus_tag="VWX16_001331"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9158674.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(12019..12090)
                     /locus_tag="VWX16_001332"
     tRNA            complement(12019..12090)
                     /locus_tag="VWX16_001332"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12055..12057),aa:Arg,seq:ccg)
     gene            complement(12154..13140)
                     /locus_tag="VWX16_001333"
     CDS             complement(12154..13140)
                     /locus_tag="VWX16_001333"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9158675.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13315..14631)
                     /locus_tag="VWX16_001334"
     CDS             complement(13315..14631)
                     /locus_tag="VWX16_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9158676.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLYINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14618..15826)
                     /locus_tag="VWX16_001335"
     CDS             complement(14618..15826)
                     /locus_tag="VWX16_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9158677.1"
                     /translation="MSENINEYKNILLQSKNLILRGAPGTGKTYLAKEIAKELTDGNE
                     DQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGGQDN
                     FDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYELYK
                     DKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISKIF
                     GELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDFAM
                     RRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPSYFL
                     KLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQAVADDN
                     EGDENDDADY"
BASE COUNT         4643 a         3594 c         2909 g         5021 t
ORIGIN      
        1 atagcaattc aaagaaagga aaaaatcaat gttatatttt ctgacacgaa aaatataaaa
       61 atttgccttt ttttactttt tctgatataa tgagagaata ttcggaaaag gagactaaaa
      121 atgaagaaaa aatttctagc atttttgcta attttattcc caattttctc attaggtatt
      181 gccaaagcag aaacgattaa gattgtttct gataccgcct atgcaccttt tgagtttaaa
      241 gattcagatc aaacttataa aggaattgat gttgacatta ttaacaaagt cgctgagatt
      301 aaaggctgga acattcagat gtcctatcct ggatttgacg cagcagtcaa tgcggttcaa
      361 gctgggcaag ccgacgctat catggcaggg atgacaaaga ctaaagaacg tgaaaaagtc
      421 ttcaccatgt ctgatactta ctatgataca aaagttgtca ttgctactac aaagtcacac
      481 aaaattagca agtacgacca attaactggc aaaaccgttg gtgttaaaaa cggaactgcc
      541 gctcaacgtt tccttgaaac aatcaaagat aaatacggct ttactattaa aacatttgac
      601 actggggatt taatgaacaa cagcttgagt gctggtgcca tcgatgccat gatggatgac
      661 aaacctgtta tcgaatatgc cattaaccaa ggtcaagacc tccatattga aatggatggt
      721 gaagctgtag gaagttttgc tttcggtgtg aaaaaaggaa gtaaatacga acacctggtt
      781 actgaattta accaagcctt ggctgaaatg aaaaaagatg gtagtcttga taaaattatc
      841 aagaaatgga ctgcttcatc atcttcagca gtgccaacta caaccactct cgcaggatta
      901 aaagctattc ctgttaaggc taaatatatc attgccagcg attcttcttt tgcacctttt
      961 gttttccaaa attcaagcaa ccaatacact ggtattgata tggaattgat taaggcaatc
     1021 gctaaagacc aaggttttga aattgaaatc accaaccctg gttttgatgc tgctatcagt
     1081 gctgtccaag ctggtcaagc cgatggtatc attgctggta tgtctgtcac agatgctcgt
     1141 aaggcaactt ttgacttctc agaatcatac tacactgcta ataccattct tggtgtcaaa
     1201 gaatcaagca atattgcttc ttatgaagat ctaaaaggaa agacagtcgg tgttaaaaac
     1261 ggaactgctt ctcaaacctt cctaacagaa aatcaaagca aatacggtta caaaatcaaa
     1321 acctttgctg atggttcttc aatgtatgac agtttaaaca ctggtgccat tgatgccgtt
     1381 atggatgatg aacctgttct caaatattct atcagccaag gtcaaaaatt gaaaactcca
     1441 atctctggaa ctccaatcgg tgaaacagcc tttgccgtta aaaaaggagc aaatccagaa
     1501 ctgattgaaa tgttcaacaa cggacttgca aaccttaaag caaacggtga attccaaaag
     1561 attcttgaca aatacctagc tagcgaatct tcaactgctt caacaagtac tgttgacgaa
     1621 acaacgctct ggggcttgct tcaaaacaac tacaaacaac tccttagcgg tcttggtatc
     1681 actcttgctc tagctcttat ctcatttgct attgccattg tcatcggaat tatcttcggt
     1741 atgtttagcg ttagcccata caaatctctt cgcgtcatct ctgagatttt cgttgacgtt
     1801 attcgtggta ttccattgat gattcttgca gccttcatct tctggggaat tccaaacttc
     1861 atcgagtcta tcacaggcca acaaagccca attaacgact ttgtagctgg aacaattgcc
     1921 ctctcactca atgcggctgc ttatatcgct gaaatcgttc gtggtggtat tcaggccgtt
     1981 ccagttggcc aaatggaagc cagccgaagc ttgggtatct cttatggaaa aaccatgcgt
     2041 aagattatct tgccacaagc aactaaattg atgttgccaa actttgtcaa ccaattcgtt
     2101 atcgctctta aagatacaac tatcgtatct gctatcggtt tggttgaact cttccaaact
     2161 ggtaagatta tcattgctcg taactaccaa agtttcaaga tgtatgcaat ccttgctatc
     2221 ttctatcttg taattatcac acttttgact agactagcga aacgcttaga aaagaggatt
     2281 cgttaatggc aaaattaaaa attgatgtaa atgatttaca caagcactat ggaaaaaatg
     2341 aagtcctaaa aggaattacg actaagttct atgaaggaga tgttgtttgt atcatcggtc
     2401 cttcaggttc tggtaagtca actttcctcc gtagcctcaa tcttttagaa gaagtcacta
     2461 gcggtcacat cactgtgaac ggctatgatt taactgaaaa aacaaccaat gttgaccacg
     2521 tccgtgaaaa tatcggcatg gtattccaac acttcaacct cttccctcat atgtctgtat
     2581 tggacaacat cacctttgct cctattgagc acaagttgat gactaaggaa gaagctgagg
     2641 aattgggaat ggagttgctt gaaaaggttg gactagcaga taaagctaat gccaatccag
     2701 atagcctatc aggtggtcaa aaacaacgtg tggccatcgc tcgtggccta gcaatgaatc
     2761 cagacatcat gctcttcgat gaaccaactt ctgcccttga ccctgagatg gttggagacg
     2821 tacttaacgt tatgaaggaa ttggctgagc aaggcatgac catgattatc gtaacccatg
     2881 agatgggatt tgctcgtcag gttgccaacc gcgttatctt tactgcagat ggcgagttcc
     2941 ttgaagacgg aacacctgac caaatctttg ataacccaca acaccctcgt ctgaaagagt
     3001 tcttagataa ggtcttaaac gtctaaactc aaactgtaag gatttccttg cagtttttct
     3061 acctcgtatt ggaatttttg atttttcgga aaattatgtt agaattaagt ttatgaaatg
     3121 aggtttcctc atacctagca agactaggaa taaaaataga aattaggtag ctagatgtca
     3181 tctaaggtta ttgttacaat tttcggtgcg agtggagacc tggctaaacg caagctctac
     3241 ccttcccttt ttagactata taaatccggc aatctttcca agcactttgc cgttattgga
     3301 actgcccgta gaccttggag taaggaatat tttgaatctg tagttgtcga gtccatcctt
     3361 gatttggcag atagtaccga gcaagcccaa gaatttgcta gccacttcta ctatcaaagc
     3421 catgatgtca atgatttgga acattatatt gctttgcgtc aattacaagc tgagcttaat
     3481 gaaaaatacc aagctgaaca caataagctc ttcttcttgt ctatggcacc tcagttcttt
     3541 ggaaccattg ccaaacacct caaatctgaa aacattgtcg atggcaaagg ttttgagcgc
     3601 ttgatcgttg aaaaaccatt tggtacagat tacgcaactg caagcaagtt gaatgacgaa
     3661 ctcctagcaa catttgacga agaacaaatt ttccgtattg accattatct tggtaaggaa
     3721 atgatccaaa gcatctttgc agttcgcttt gcaaacttga tttttgaaaa cgtttggaac
     3781 aaggatttta tcgacaatgt tcaaattacc tttgcggagc gcttgggtgt agaagaacgt
     3841 ggtggctact atgaccaatc cggtgccctc cgtgacatgg tccaaaacca cactctacaa
     3901 cttctttcgc tcctcgccat ggacaaacca gcaagcttca caaaagacga gattcgtgct
     3961 gaaaagatta aggtctttaa aaacctctat catccaactg atgaagaact caaagaacac
     4021 tttatccgtg gacaataccg ctctggtaag attgatggca tgaaatacat ctcttatcgt
     4081 agcgaaccaa atgtgaatcc agaatcaaca actgaaacct ttacatctgg tgccttcttt
     4141 gtagacagcg atcgattccg tggtgttcct ttctttttcc gtacaggtaa acgactgact
     4201 gaaaaaggaa ctcatgtcaa catcgtcttt aaacaaatgg attctatatt tggagaacca
     4261 cttgctccaa atattttgac catctatatt caaccaacag aaggcttctc tcttagccta
     4321 aatgggaagc aagtaggaga agaatttaac ttggctccta actcacttga ttatcgtaca
     4381 gacgcgactg caactggtgc ttctccagaa ccatacgaga aattgattta tgatgtccta
     4441 aataacaact caactaactt tagccactgg gatgaggttg gtgcgtcatg gaagttgatt
     4501 gaccgtattg aagagctctg ggccgaaaat ggtgccccac ttcatgacta taaagctgga
     4561 agcatgggac ctcaagccag ctttgaccta cttgaaaaat tcgatgccaa atggacttgg
     4621 caaccagata tcgcctatcg tcaagatggt cgtttcgaat aaaaaaatcc tgcaagtttg
     4681 taccttgcag gatttttgct tctgattaga ttaagccttc caagagacct ttcataaagt
     4741 tttctgagtt aaactctcca atatcatcga ttttttcacc aaaaccaatc aattttacag
     4801 gaatattgag ttcttcacga atggctagaa ccacacctcc tcgagcagtt ccatcaatct
     4861 tagtcaaaac aattcccgtt aaaggtgtga ttttcgaaaa ttctttggcc tgtactaggg
     4921 cattttgacc tgttgatgca tcaagtgcca agaaggtttc atgtggtgct tctggcacaa
     4981 cacgtttgat aatacgacca atcttttcca actcagccat aaggttatcc ttattttgca
     5041 gacgaccagc agtatcaatc atgagaatat cgataccttc agccacggca cgttccatac
     5101 catcaaagac cacgctggct ggatcagctt tttcaggtcc agttactact ggaacatcta
     5161 ctcgtcggcc ccattcagct agctgagcta ctgcacccgc acggaaggta tctgctgcaa
     5221 ccagcatgac cttcttacca gttcgtttgt agcggtgggc tagttttcca atagaagttg
     5281 ttttcccaac accattcaca ccaacaaaga gcataactgt caagttatct tggaagtgga
     5341 tgctttcatc gtagctacca tccttttcat aaagctcaac caatttctca atgatgacac
     5401 gacgaagtgc atcaggtttc ttggcatttt caagcttggc ttcgtaacgt agttcctccg
     5461 ttaagttaga agcgacttgg acaccaacat cactcataat cagcagttct tccagttcct
     5521 cgaaaaattc ttcgtcaaca gagcggaagt tagcaaagaa ggcgttcaag cgagcaccga
     5581 agcccgtacg agtcttcttg agactgcggt catatttttc ctgaacagtt tcttctgttt
     5641 gaggcctttc tggttcaaga acttcagaat tattttcttc tatagttcct tcgtgctcaa
     5701 gcttctcttc ctctggtaat tcttctgagt ttggtaattc ttctatttct tcttgagaaa
     5761 cccctacagc tggctctgaa tcctgacttt cttcaactgt gtcttggatt tcctcctctt
     5821 ggaacacagc ttgttcaaca atttcaacct ctgcttcttc ctgaggaact tcctcaactt
     5881 ctgtgaaggt aggatcaaca tcttcagaca aatcaagatt ttccagagct tcttttacaa
     5941 cttcttcgat tttaggttct tctttttttc cgaatagacg gtcaaacaat cccatatctt
     6001 agttctcctt tagcacatat tcttcgatag cccaggcgac agcttcctca tcgttggtca
     6061 tcggcgttac tacatttgcg gctgccttta cttcaggaac agcgttttgc atagcaacac
     6121 caagacctgc ccattcaatc atagaaaggt cattggcctc gtcaccacag gccataactt
     6181 ggctttggtt gattccaaga tggctgatta gttttgccaa acctgttgct ttatgaacat
     6241 tctttggtga ccactctagc aacatttcac gtgatttaaa gatttcatat tggtcaaaca
     6301 attctggaga aatcttctga atggctgcat ccaagggttc ttgagcaaag gcagtcacgc
     6361 atttgttgta ggtcatttga ctagacaggt cttcaaagtc aactggaaca aaagtcaaag
     6421 ctggattgaa tttggcataa agactttctt ggtccgattg gatttgataa actgttcctt
     6481 ctgagatggc atcaagtggc agtgataatt tctctgtttt ctcatacaag cgtgccacat
     6541 cagcatatga aaagactgtt ttatcaagga tttcacctgt atttttctga accaaaccac
     6601 cattaaaagt aatggtatac tcatcttcct gaccgtcagt ccctaactca tggagaaaga
     6661 agtccatggc ttttaaggga cgaccagttg tcaatacgac cttgatacca cgatcacgcg
     6721 cagctttcaa agttgccttg gtgcgatccg tcagcctttt atcagtagtc agcaaggtac
     6781 cgtccaagtc caatgcgatt aattttatat ctgccattat aagccctcca tataagctat
     6841 aaccgactgg tccttatggt gaccaatcac agtctttgct aattctaaaa tctctggtcg
     6901 tgcattttca ggagctacag gatgtcccac aacctgcatc atatgtaagt cattaagatt
     6961 gtctccaaaa gccatgacct gatccattgt gatgccaagt tttttagcta attcaacaat
     7021 agctacaccc ttatcgacat agtccagaac aatatcaata gattcaaagc cagttgtcat
     7081 agccttgaca ccagggacat tatcattgac ccaagcttca ccagcttcta gcgtttcttc
     7141 tgtgaagttg gttgtaaatt tgaaaatgtc atctgtgata tcttccaaac tcgctacttt
     7201 ttggatattt tcattataat gctgactcac tttcaaatag gtctcatcaa ccgtatctag
     7261 aacatatgaa cccttcttac ccgtcaagag cagtttattg atatctatat aaggtgacgt
     7321 tttcagcttt tcaaaagttg ccagataaaa gtcacgagac atagtcgctt catacaagtc
     7381 ctgaccttga tactctacca aactgccatt ttccgcgatg aaaataatgt catcacgaac
     7441 accagcaaat aatttttcca gagacagaaa tccccgaccc gaagccaccg caaagtaaat
     7501 ccctttttcc ttgtaggaaa ccaagagaga cttgaggcgg tccatatcaa agcgcccatt
     7561 ctcatctagg aaggttccgt ccatatccgt tgctactagt ttaattgtca tccttcaata
     7621 ctttctaaat cttttaactt aactgaaaca atctttgaaa cacccgattc ttgcatggtc
     7681 actccataga tggaatcggc cgctgccatg gttcccttac ggtgggttac gacgataaac
     7741 tggctgtcct tgtcaaagcg gttgaggtaa tccccaaaac gtttaacatt ggcttcatcc
     7801 agcgcagctt ccacctcatc caagatgaca aaaggaatgg tcttgacacg aataatggag
     7861 aaaagcaagg caagagccga tagggctttt tcaccaccac tcatgaggtt aagcgactgg
     7921 attttcttac ctggaggttg aacagaaatc tccacaccag ctgttaaaag gtcgccctca
     7981 gtcaatatca agtctgcctg acctccgcca aacatctgct tgaaggtcac tttaaaggac
     8041 tcacgaatag cttcaaaggt tgatttaaag cgttccttaa cctcatcatt catctttgta
     8101 atggtttcaa ggagcagatt tttcgctgac aaaatatcat ctcgctgact atttagaaag
     8161 tccagacggt tgtgaacttc ttcgtaccgg tcaatagctt ctatattgac aggacccagt
     8221 gagcgaatag ccttctctaa atccttaact tcttgctctg ccagattgag gttttccaat
     8281 tcatgcgctt tttctagagc ttcagtatag ctaatctggt actggtctgt taattgactt
     8341 tgtagatggc gcaagcgctc gctgaccttt tctttcttag cttcagcacg tgtttgcttg
     8401 cgaatccact cctcattctg ctggcgagcc tgatccaaat gactagcaat atcatccagc
     8461 tgaccttcta tatcatccaa ctcaaactgt ttgcgaatca aaccttgttg gaggttcgtt
     8521 ttctgagttt tggattcttc cgcctgttga ctgagcaatt ctgtatcaac cttctcaaga
     8581 ttgtcaacct tttcttgaag aaggcgctgg atttcctctt gttcaaaatc aagattgtcc
     8641 aattccttgc ctaagcgttc aatatcagca acttcataac gtttttgccc ttgcagttct
     8701 gtcttaagca aacgagcttg cgctagctct tcctgcaagt tttgatagcg ttcttggatg
     8761 gcatttttat tagacttaat ctcttcaatc tcagcttcca gattttgctt gtcactggcg
     8821 attgcagcaa gacgctcttg gcatttttcc ttatccgctt gccaatctcc ctcggaaaga
     8881 cgatctattt cctcttcttg gagtttccaa agagtttcca gttcttcaac ttgctgacta
     8941 gtttgctgat aagcgaggga caagccttgc tcctgaatac gtgcctgctc tccttgagat
     9001 ttaatagctt ctaatgactc ggtcaatctg gccatctcat cttgcaaggt cttcaaagcc
     9061 acttcttctg aacccaagct tgcttcttct tcagcaattt ctttttgtaa ttgctccagt
     9121 tctggcttga tgaagatgct gttgttctga cgattggctc cacctgcata ggaaccacct
     9181 gtacgaagct ctgtcccatc caatgtcacc atgcgaacct gataacgaac ttgtcgagca
     9241 gcttcacgcg catgttctac ggtatcaaag atagccgtcg tagctagcaa gttcttgaaa
     9301 atggcttcca gtctagtatc aaaagtcacc aactcatctg ccatcccaag gaaacctggg
     9361 cttacagcga tagcatcttg gttctgacta gaaatcgtac gcgccttaat agtggtcaaa
     9421 ggaagaaagg ttgcacgacc ggctctgttt cgtttgagga aatcaatagc tttggttgcc
     9481 gactcttcat cttctacgat gatatgctgg ctacttgccc ctaaggcaat ctctagggca
     9541 gtttgataat aaacatcaaa ggtcagatgc tcactgactg caccaataat cccacctagg
     9601 cgatcttttt cttggagaac actcttaaca cctgcataaa agttgctatg atttctcagg
     9661 atattttcca aactttgagc tctggcctgc ttgtttttga gactatccag acggtcaaag
     9721 agttgagttt gttgagcttg ataggaagtt ttctgctcct cttgctcctt ggcaatagct
     9781 tggtagtcag ccaataattt ctgaacctgc tccttggcag tttcaagctc ttccttttgc
     9841 tgactagcct tctctttagc tatagctaat tgctctttca gcttttctag ttgatctgct
     9901 tgtttttgag aaagctgacg actattttcc aactcattct caatacgggt caactggttt
     9961 gagacatccg cttcttcttg taaaagagct acaaagcgtt cacgtaagag ctcaatcatc
    10021 tgatcaggat cgtctgagaa agccagcaat tcagcttcta aacgattgag tttttgatta
    10081 ttttggacta gatttccctc taacagagct aaagagcttt ctttatcaga cttttctttg
    10141 ctgagtgaat ttctcttatc ctccaaagca gccaaacggg cttgtgcctc ctgttgattc
    10201 agggccactt gctcggactc cagtttcgat agggctaatt ttctttctaa atcactaatc
    10261 agactagtca agtccatcaa actgccttgg tctttggcca tttcagcctg taaatcttgg
    10321 cgttgctttt taagagtttg attttcttct tctaattttt cacgcttttg gtaataactc
    10381 atcaagagtt cttgaacctg agccaactct tcttctgtcg actctagttc tgccttattt
    10441 tccttgattt gagcaaccag aacgtctaaa taaatagcct tacgttgtcc ttccaagtct
    10501 aaaaacttac gggcattctc agcttgcttc tcaagaggct tgatttgatt atccaactcg
    10561 tagataatgt cctctaagcg gtccagatta tcctgagttt gttgcagttt actctcagtt
    10621 tcttttctac gagtcttgta ttttaaaact cctgcagctt cttcaaaaat agcccggcgt
    10681 tcctcaggct tagaattaaa aatctcctca accttgcctt gggaaatgat agagaaggaa
    10741 tctcgtccca atccagtatc caagaagatg tcatgaatat cacgcagacg gactttcttg
    10801 ccgtcaatct tgtattcgct atctccacta cgatagatat ggcgttccac cctgatttct
    10861 tgacctgcat ccttgataaa tccgtcatga ttatccagag tcacaactac agaagcataa
    10921 ttgagcggtt tgcgactttc ggttccagca aagatgacat ccggcatctt gcccccacgg
    10981 agactcttga cactagactc ccccaaagcc caacgcagac tttctgtaat attggacttt
    11041 ccagatccat tgggtccaac aactgccgtc acaccttggt caaaaacgac cttggtctta
    11101 tcagcaaaag acttgaaccc ctgaatttcg atttccttta aatacatgaa tccagcccct
    11161 tctcaacggc atttttggca gcttcctgct ctgctaattt cttagaacga ccttggcctt
    11221 gaccgatgct cttaccttca acaagaactt ctacatcaaa gaccttatcg tgagcaggcc
    11281 ctgtttcaga aatcacctga taacgaatag ccacatcacc attgacctga agcaactctt
    11341 ggagatgggt tttatagtct gtaatcatct caaactcgcc tgcttcaacc ttaggaatca
    11401 tgacttgata gataaattcc ttgaccttgg ccacatcctt atccaaaaga agggcaccaa
    11461 gaaaggcttc aaaggcatca ccaagaatgg tgtcacgatt gcgaccacca gatttttctt
    11521 cccctttacc caacttgata aactggtcaa actggcaatc acgcgcaaaa ccagctaaac
    11581 tctcctcacg gacaatcata gcacggagtt ttgataggtc accttcaggc tttttaggat
    11641 attttttata tagatattct gaaatcaata actgtagaac agcgtctcct aaaaattcca
    11701 agcgttcatt gtgtgaaatt tttaagaggc ggtgctcatt ggcataactc gtatgagtaa
    11761 aggcagtttc cagtaaattt ttgtctgcaa attcgattgc aaaatgattc tttagtacag
    11821 tttgtaattc tttcatacca acctctttct aactgataat agtccttttt attatatcaa
    11881 aaaaagcccc ctgagtcact ctaaaacggg actggaaagc atttgggaat tctttagaca
    11941 gagattctca gttttagcga caaatttggg tcaggataaa gaaaaaagcc ctattaaagg
    12001 ctttttagga tgtttacatc caccctgagg gaatcgaacc cccatctcaa gaaccggaat
    12061 cttacgtgat atccattaca ctaagggtgg aaacttgttt tattataaca gaaatttgct
    12121 ctaataacaa gttttttggt caaagacccc gtcttagtgg gaagcatccc cattccagat
    12181 ggagtttttc acgatcacat aatcaacgtg tttaaggtca gcaacctgac gtccacctgc
    12241 ataagaaata gcactttgaa ggtcttgttc catctcagtt aaagtgtctt gcagatgacc
    12301 tttagcagga agcaagatac gtttgccttc cacatttttg taagcacctt tttgatattg
    12361 tgaggctgaa ccataatatt ctttgaactg ttcaccatcg acttcaatcg ttttccctgg
    12421 actttcaatg tgtcctgcaa agagggaacc aatcatgatc atgctagcac cgaagcggat
    12481 agacttagca atatcaccgt gagtacgaat tcctccatca gcgataatcg gtttacgcgc
    12541 agccttggca caccagcgta gagcagccaa ctgccaacca cctgtaccaa aaccagtctt
    12601 aaccttggtg atacaaacct taccaggacc gattccgacc ttagtagcat ccgcaccagc
    12661 attttccaat tcacgcacag cttctggtgt tcccacattt ccagcaatga caaaggtatc
    12721 tggcaattct ttcttgatgt gttgaatcat agaaatcacg ctatccgcat gaccatgagc
    12781 aatatcaata gtgatatact caggagcatc agccttgagc tggctaacaa aatcatactc
    12841 ataatcctta acaccgacag agatagaagc aatgagccct tgatcgtgca ttcgtttaat
    12901 aaaaggaatg cgtcctgcct catcaaaacg gtgcataatg tagaagtaac cacctttagc
    12961 cagttgctct gctacatttt catccaaaat cgtctgcata ttcgctggca caacaggtag
    13021 tttaaaggtg tgatttccta aagtgacact tgtatccgct tctgcacggc ttttaatgac
    13081 acatttattt ggaatcaatt gaatatcttc gtaatcaaaa attggaaatt catttaacat
    13141 atcgatgtct cgtttctttt gtaatgacct acctatgctc ttgcatcact acgccttttc
    13201 cgacgtttcc ttaatttatt ataaaccaaa gtacagtttt tgtcaaatta ttttatgaat
    13261 taaaatatat cgttcgggtt ttacttgtgt taaaaataaa agaaagaggg gaaattattt
    13321 tctccccact tcttcatcaa taatcgcttt aaaattttct tctgaatttt ccatcatttt
    13381 acaaaatgta ctatagaatg aggcattctg agggattcga atagaccact tcttcaattg
    13441 agctccatag ccagctactt ttcctatttc actatttact gactgctcca tactaggaaa
    13501 aatcagtcca gccttctcag cttttaaaat ataagaatag gaaatcagct ggaataagtc
    13561 ctcacggttg attccttttt cagtcaattc cagtttttta tattttgcat ctagaacaat
    13621 ctttcgttct ctgtcataaa aatctggata tacttttcgt ttcccaacag aaaatactga
    13681 aattccatcc gtcttatcct tatttctggg atgtacaaaa ccttttggca acaaggtgta
    13741 aacatactct tcccaaagcc aggcaacatc aaagagaata ccatagattt tttgatcttg
    13801 ataccctaaa ccgtgctttt cttggtttag gatcatcaga caaagttctt gtaagtttct
    13861 gtactcgtga aagtatgcat gacgtatagg tttagattga tttccccgaa taatcttagc
    13921 acgatcagct agtttataag agggcgttac acgcacgatt tcagatacgt tttcacgact
    13981 agttgagaga ttatctagta ccccttgacc aatgcttttc tgattcttaa tgtattcaat
    14041 agtgtgacgg accaactgca tgaggggatt atcataggtg aactctctcg ttgcgtaggc
    14101 aatatttccc gtgaaaggaa gatttttctt gagatggttt cttacatcaa tcactccctt
    14161 aacatgactg tcgttatgag aaaatcgatg atattcctta taaagacctt ttcgaatagc
    14221 agcttgtaga tacttgggaa agagatacac caaaagttga taaagcctct cttcacgaga
    14281 caaagcaaca tctaaactag tgagattgat ataaagaacc ttgtttaaga gataatgcaa
    14341 aaaatggtca ttactctcat cagaaaatcg tgaggaaatc gttaatcttt cctgaccata
    14401 tccaagaaaa ccaatcacgt tccctgtctt gattttctga ttgactgttt caaaaatctt
    14461 ttggtcctta tccaaatcag gagtatgagt caaatcattt ggaaaaatga aaatacgttc
    14521 atcttgggat agactttcca aggttctgtc taaaagaaga ttgcttagtt taggatattc
    14581 ttcaacaaat ttttctttaa taatcttgtg ttgattatca gtaatccgca tcatcgtttt
    14641 catcgccttc attgtcatca gctactgcct gatctttttg ctcattattt gtcagttcaa
    14701 atgctttttt caaagtttcc aaagtttcaa cctcatcata agaacctcgc aagtagtctt
    14761 ctaggagagg cttaatataa tcagaccaga gtaattcata gtcaaaatct acatccttca
    14821 acttaagaaa ataacttggt ccaatatgat aatgactgtt taattcctga acattttcga
    14881 tagcagcgtt caagtttctt agacgaattt ttgcttcttc tgcatggata ttcagctctt
    14941 tatccaacat gccaacttga ccctcggcag taacttcaac aaaacgaaaa cgacgacgca
    15001 tagcaaaatc aaaggtatcc actgaacgat caatgtcatt catagttccg atgatgtaga
    15061 cattttcggg gatatagaac ttttcatcag tttcgtgtag atttgcatat tgggtagaaa
    15121 cacttccttt ttcaccacga tagccagggt cgatagagaa aaagagttcg ccaaaaatct
    15181 tagaaatctc cccacgattg atttcatcga tgatgaagac gaatttctta tcagtatcaa
    15241 tttctgttgg ggaaacatag tctttcaaac caaatctctt tctcaatgtt tctaggacag
    15301 tttttccacc acttttgtag tattcttgct tattataatt tttatcttta tacaactcgt
    15361 aaacatattt gctaggtagt gaccatcctg gaacaccact atcataattt actgacaaat
    15421 tttgtctact attaacagat aagtaagatg tttttgttat atattctttt tcttcagcaa
    15481 catttatata ttctaagtaa gaatcccaag cctcatcaaa attatcttgt cctccaatca
    15541 attgggtttc ttttgctttc tgacaaaaat ctttaaaaat accgtcctgt agcctaaact
    15601 caatagctcc atccccattt gatactggtc ttaaaccttc tacaaaatcc gtataatcat
    15661 aggatgggtg aaattgtaca aatccgattt ggtcttcgtt gccatccgtt aattctttgg
    15721 caatttcttt agcaagataa gtctttcctg tgccaggagc accacggagg atgaggtttt
    15781 tggattgtaa taagatattc ttatactcgt tgatattttc tgacatgttt atctcttcct
    15841 tttctgattc tctataaaaa ctagtgaaat tatcataaaa ctcttttaaa atatcgttag
    15901 gttcagcagt tgattccaac tctaagtcct tgacaaagta tttataatct aaatcttcat
    15961 ctcgattatt actatacctt tttactattt taaattctgt gagtttaaat ctctgaaaac
    16021 cacatcttat atttatccat gagttatcca tgtcactcag ccgataacca aagtaaggtg
    16081 atttaatata tccattacca ctctttggac caaaatgacc tgtgttaagc atttcaactt
    16141 tgtacagaat accctcaata gtcagag
//
LOCUS       DAVKAO010000039        15419 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKAO010000039 DAVKAO010000000
VERSION     DAVKAO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.039"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            183..1970
                     /locus_tag="VWX16_001336"
     CDS             183..1970
                     /locus_tag="VWX16_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9158678.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRNPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1967..2443
                     /locus_tag="VWX16_001337"
     CDS             1967..2443
                     /locus_tag="VWX16_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9158679.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2471..3376
                     /locus_tag="VWX16_001338"
     CDS             2471..3376
                     /locus_tag="VWX16_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9158680.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3363..4178
                     /locus_tag="VWX16_001339"
     CDS             3363..4178
                     /locus_tag="VWX16_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9158681.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFASLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4185..4589
                     /locus_tag="VWX16_001340"
     CDS             4185..4589
                     /locus_tag="VWX16_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9158682.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIGGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4883..6049
                     /locus_tag="VWX16_001341"
     CDS             4883..6049
                     /locus_tag="VWX16_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9158683.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVFSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6165..7202
                     /locus_tag="VWX16_001342"
     CDS             6165..7202
                     /locus_tag="VWX16_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9158684.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7408..8262
                     /locus_tag="VWX16_001343"
     CDS             7408..8262
                     /locus_tag="VWX16_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9158685.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIAQRAQEVDVPIKWMTSSQLEITDSDGI
                     VTCIRLAR"
     gene            8412..8669
                     /locus_tag="VWX16_001344"
     CDS             8412..8669
                     /locus_tag="VWX16_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9158686.1"
                     /translation="MTNSKYITCLKRSDGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8711..9028)
                     /locus_tag="VWX16_001345"
     CDS             complement(8711..9028)
                     /locus_tag="VWX16_001345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158687.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDRTDEVSNHIYGKVKLTWFEEI
                     FEKYKSPHNKTHITKVLMNCTPKVRQKKSNFLGSVHSTPDTMRFFNFKDFLQLLLTPI
                     KLF"
     gene            complement(9082..9378)
                     /locus_tag="VWX16_001346"
     CDS             complement(9082..9378)
                     /locus_tag="VWX16_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9158688.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNVWPGQVE"
     gene            complement(9368..9529)
                     /locus_tag="VWX16_001347"
     CDS             complement(9368..9529)
                     /locus_tag="VWX16_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9158689.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            9599..10363
                     /locus_tag="VWX16_001348"
     CDS             9599..10363
                     /locus_tag="VWX16_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9158690.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            10928..12307
                     /locus_tag="VWX16_001349"
     CDS             10928..12307
                     /locus_tag="VWX16_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9158691.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSFRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12321..12986
                     /locus_tag="VWX16_001350"
     CDS             12321..12986
                     /locus_tag="VWX16_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9158692.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13100..13195
                     /locus_tag="VWX16_001351"
                     /pseudo
     CDS             13100..13195
                     /locus_tag="VWX16_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            13386..13924
                     /locus_tag="VWX16_001352"
                     /pseudo
     CDS             13386..13924
                     /locus_tag="VWX16_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            13991..14401
                     /locus_tag="VWX16_001353"
     CDS             13991..14401
                     /locus_tag="VWX16_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9158693.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            14517..>15419
                     /gene="pavB"
                     /locus_tag="VWX16_001354"
     CDS             14517..>15419
                     /gene="pavB"
                     /locus_tag="VWX16_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9158694.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETV"
BASE COUNT         4295 a         2904 c         3538 g         4682 t
ORIGIN      
        1 attccgaatt taaagagcgt ttgaaatggg tctaaaaatg tttgaaacta attgaatgtg
       61 tgtataatag taaaaatgca gtaaatatcc cgacaatttc tattgacaat tcaaacagat
      121 tggtttataa ttaatataac aacaaatgaa agcgcaaact ttcgtggtcg gaaggtagtt
      181 ttatgacacg atttgagata cgagatgatt tctatctcga tggaaaatca tttaagattt
      241 tatctggtgc cattcattat tttagggttc ctccagagga ttggtatcat tcgctctata
      301 acttgaaggc tcttggtttt aatacggtag agacttatgt tgcttggaat ttacacgagc
      361 ctcgtgaagg tgagtttcat tttgaaggtg atctggattt agagaaattt ctccaaatag
      421 cgcaggattt gggtctctac gcaattgtgc gtccgtctcc atttatctgt gcggaatggg
      481 aattcggtgg cttaccagct tggctcttga ccaagaacat gcgaattcgc tcatccgacc
      541 cagcatatat cgaggcagtt ggtcgctact atgatcagtt attgccaaga ctggtgcctc
      601 gtttgttgga caatggtggc aatattctca tgatgcaggt tgaaaatgag tatggttctt
      661 acggagaaga taaggcttac ctgagagcga ttcgacagct aatggaagag tgtggcgtaa
      721 cctgtcccct ctttacatca gatggtccgt ggcgagctac tctgaaagct ggaaccttaa
      781 ttgaagagga cctctttgta acaggaaact ttggttctaa ggcaccttac aacttttcgc
      841 agatgcagga attctttgat gaacatggta agaaatggcc actcatgtgt atggagttct
      901 gggatggttg gttcaatcgc tggaaagaac cgattatcac acggaatcct aaggaattgg
      961 cagatgcagt tcgagaggtt ttggaacaag gctctatcaa tctttacatg ttccacggtg
     1021 gtacaaactt tggtttcatg aatggttgct cagctcgagg aactttggac ctgccacaag
     1081 ttacgtctta tgattacgat gcccttctgg atgaagaagg aaatccaact gctaaatatc
     1141 ttgcagtcaa gaagatgatg gcaacacatt tttcagagta tccgcagttg gaaccactct
     1201 acaaagagag tatggagttg gatgctattc cactagttga aaaagtttct ttgtttgaaa
     1261 ccttagatag cttgtcaagt cctgtagaaa gtctctatcc tcaaaagatg gaggagctgg
     1321 gacaaagtta tggctaccta ctttatcgaa cagaaacaaa ctgggatgca gaagaagaaa
     1381 gacttcgtat cattgatggt cgagataggg cccagctgta tgtcgatggt cagtgggtta
     1441 aaactcaata tcagacagag attggggaag atatttttta tcaaggtaaa aagaaagggc
     1501 tatctaggtt agatatcttg atagaaaata tggggcgtgt caactatggg cataagttct
     1561 tagcggatac gcaacgtaag ggaattcgga caggggtctg taaggatctg catttcttac
     1621 taaactggaa acactatcca ctcccactag acaatcctga gaaaattgat ttttcaaaag
     1681 gatggactca aggacaacca gccttttacg cttatgactt tacagtcgaa gagccaaaag
     1741 atacttacct agacttgtct gagtttggta agggggttgc ctttgtcaat gggcagaatc
     1801 taggacgttt ttggaacgtt ggcccaactc tctcacttta tatccctcat agctatctca
     1861 aggaaggtgc caaccgcatc attatctttg aaacagaagg tcaatataaa gaagagattc
     1921 atttaactcg taaacctaca ctaaaacata taaaggggga aaacttatga caattgtagg
     1981 atgccgtatt gatggacgtt tgatccacgg acaagtagcc aatctttggg ctggaaaact
     2041 aaatgtttca cgcattatgg ttgtagacga cgaagttgtc aacaacgata ttgaaaagag
     2101 tggtttgaaa cttgcgacac caccaggtgt gaaattgagt attttgccag ttgagaaagc
     2161 tgcagccaat attcttgctg gcaaatacga tagccaacgt ctctttatcg tggctcgtaa
     2221 accagaccgc ttccttggtt tggtagaagc aggtgtacca cttgaaaccc ttaatgttgg
     2281 gaatatgtct caaacaccag aaactcgttc tattacacgt tctatcaacg tagtagacaa
     2341 ggatgtggaa gacttccaca aactggcaga aaaaggtgtt aaacttactg ctcagatggt
     2401 tccaaatgat ccaatttcag actttttgag cttattaaaa taggaaaaaa atttttagga
     2461 ggtcattgtt atgatacaat ggtggcaaat tttacttctc actttgtact cagcttatca
     2521 aatctgtgat gagttgacga tcgtttcatc tgcaggttcc cctgtatttg ctggtttcat
     2581 tactggttta atcatgggag atgtgactac tggtttactt atcggtggta acttgcaact
     2641 gttcgttctt ggggttggta ccttcggtgg tgcttctcgt atcgacgcaa cttctggtgc
     2701 ggttcttgcg acagccttct ctgtttcaca aggaattgat gcaccgcttg ccattactac
     2761 aatcgctgta ccagtagcag ctctcttgac ttacttcgac gttcttggtc gtatgactac
     2821 taccttcttc gctcaccgtg tggatgctgc aatcgaacgc tttgactata aaggtattga
     2881 acgcaactac ttgcttggtg cgattccgtg ggctctatct cgtgcccttc cagtcttctt
     2941 tgcccttgct tttggtggtg cctttgtaca atcagtagta gacttcgttg aagcctacaa
     3001 atgggttgca gatggcttga cacttgcagg acgtatgctt ccaggtcttg gatttgcaat
     3061 cttgcttcgt taccttccag ttaaacgtaa ccttcactac cttgctatgg gatttggttt
     3121 gacagctatg ttgactgttc tttactcata tgtaacaggt cttggtggcg ctgttgctgg
     3181 tatcgtaggt actcttcctg ctgaagttgc tgaaaaaatt ggtttcgtga acaacttcaa
     3241 aggtttgtct atgattggta tttctatcgt aggtattttc cttgcagtgc ttcacttcaa
     3301 aaatagccaa aaagtagctg tagcagcacc ttctacacca tcagaaagtg gggaaatcga
     3361 agatgacgaa ttctaattac aaacttacaa aagaagattt taatcaaatc aacaaacgta
     3421 gcttgtttac tttccaatta ggttggaact acgaacgtat gcaagcttct ggttaccttt
     3481 acatgatctt gcctcagttg cgtaaaatgt atggtgatgg aactcctgaa ttgaaagaaa
     3541 tgatgaaagt tcatactcaa ttcttcaata cttcaccatt cttccatacc attatcgctg
     3601 gttttgacct tgccatggaa gaaaaagatg gtgtaggttc aaaagacgcc gttaacggta
     3661 tcaagacagg tttgatggga ccattcgctc ctcttgggga tacaatcttt gcttcacttg
     3721 tacctgctat catggggtca gtcgcagcaa ctatggctat cgctggccaa ccttggggga
     3781 tcttcctttg gattgcagtt gcagtagcgt atgacatctt ccgttggaaa cagttggaat
     3841 ttgcttacaa agaaggggtt aaccttatca acaacatgca aagtaccttg acagctttga
     3901 ttgacgctgc atctgtactt ggtgtcttca tgatgggtgc tcttgtagca acagtgatta
     3961 actttgaaat ttcttacaag ttgccaatcg gtgaaaagat gattgatttc caagacatct
     4021 tgaatcaaat cttcccacgt ttgcttccag caatctttac tgcctttatc ttctggttgc
     4081 ttggtaagaa aggtatgaac tctactaaag ctatcggtat tattatcgta cttgctttgg
     4141 ctctttctgc ccttggtcac tttgcacttg gaatgtaatt ccttatgact aaatcattaa
     4201 ttttggtgag ccatggtcgc ttctgtgagg agcttagagg tagcacagaa atgattatgg
     4261 gcccacaaga caacatttac acagtagctc ttcttccaga agatggccca gaagaattta
     4321 ctgctaaatt tgaagctgtt attggaggat tggatgattt cctagtcttt gcggatcttc
     4381 tcggtgggac accttgtaat gtggtgagtc gcttgatcat ggaaggtcgt gatattgacc
     4441 tttacgcagg gatgaatctt ccaatggtga ttgaatttat caatgcgagc cttacaggcg
     4501 cagatgcgga ctacaagagc cgtgctgcag aaagcattgt gaaagttaat gacctgttag
     4561 cgggcttcga tgatgacgaa gatgaataat actcttcgaa aatctcttca aactacgtca
     4621 acgtcgcctt gccgtaggta tatgttactg acttcgtcag tcttatccgg caacctcaaa
     4681 acggtgtttt gagctgactt cgtcagttct atctgcaacc tcaaagcagt gctttgagca
     4741 gcctgcggct agtttcctat tttagttgga actcgattca attcatgtga caacgtgaaa
     4801 atcgttagag cattttatat agaatataca tgggaatgga gcttactccc attcccatat
     4861 ttaatagaaa aagaggaact caatgctaca ttatacaaaa gaagacttgc tcgaattggg
     4921 tgcagaaatc actacgcgtg aaatctacca acagcctgat gtatggagag aagcttttga
     4981 attttatcaa gcaaaacgtg aagaaattgc agccttccta caagaaatcg ctgataaaca
     5041 tgactatatt aaggttatct tgacaggtgc tgggacttct gcttatgtgg gagatacctt
     5101 gctaccttac tttaaggaag tctatgacga acgcaaatgg aatttcaatg ctattgcgac
     5161 aacagatatc gttgccaatc cagcaaccta tttgaaaaaa gatgtggcaa ctgtccttgt
     5221 gtcttttgct cgtagtggga actcgcctga aagtgtggcg actgttgatt tggccaaatc
     5281 cttggtggat gagctttatc aagtgacgat tacttgtgca gcagatggta aattggctct
     5341 tcaagctcac ggtgatgacc gtaatctctt gctcttgcaa ccagctgtct ctaatgatgc
     5401 tggatttgcc atgacttcta gctttacgtc tatgatgttg acagctctct tggtctttga
     5461 tcctacagaa tttgctgtta agtctgaacg ttttgaagtt gtatttagtc ttgcccgtaa
     5521 agttttagac aaggcagaag atgtcaaaga gctcgttgat ttagacttta accgtgtcat
     5581 ctatctaggc gctggtcctt tctttggact tgctcatgaa gctcagctca agattttgga
     5641 attaactgct ggtcaagttg cgaccatgta tgaaagccca gttggcttcc gtcacggtcc
     5701 aaaatctctt atcaacgaca atacagttgt tttggtcttt ggtacaacga cggactacac
     5761 tcgtaagtac gacttggact tggttcgtga agttgctggt gaccagattg ctcgtcgtgt
     5821 tgtgcttttg agtgatcaag cttttggtct tgaaaatgtc aaagaagtgg cccttggttg
     5881 tggcggtgtc ttgaatgata tttaccgtgt cttcccttac atcgtttatg cccaactctt
     5941 tgccctattg acttcactca aggtagaaaa taaaccagat acaccgtctc ctacaggtac
     6001 agtaaaccgt gtagtacaag gtgtcataat tcacgaatat caaaagtaag acagtgttta
     6061 tgaattcttg acaagaggat ttgtaaatta tcagataaac catagattgt cagtacgctt
     6121 tctatggttt gtttgcttga gagaaatagt aaaaggagaa cagaatgaaa gcatacacag
     6181 agcgtgtatt tggaaatgtt gagggtgagg atgtcttggc ctatcgattt gagacagacg
     6241 gtggctacca acttgaggtt atgacttatg gtgcgactat cttgcgctat gtcgcacctg
     6301 acaaggctgg aaattttgcc aatgttatct tgggatttga tgactttgat agttatgtag
     6361 gcaatagtcc caagcatgga gcaagtgtag gtcctgtagc gggtcgtatt gcaggtgcga
     6421 cctttgagct caatggtaag acctatgacc ttgaggttaa taatgctagc aactgtaatc
     6481 acagtggttc aactggttgg gattccagct tgtttgaagt tgaagaagta agcgatcatg
     6541 gcttgactct ctacacagag cgtacagatg ggacaggagg gttccctgga aatctcaaga
     6601 tttggatcag ttatcacttg gaagaaactg gtgcctatga aatcagctac aaggtaacga
     6661 ccgatcagga tacgctggtc aatccaacca accacagcta tttcaacttg tctggtgatt
     6721 tcacgcagac gattgaccgt catgtcttcc aactaaacac agagggcatt tactcaatcg
     6781 ctcctgacgg tgttcctgcc aaaactccag aagccaaccg tgatgtggtc aaacacatct
     6841 acaatggtgc cttgttgaag gatatctttg cagaagaaga tgagcaaatc cagctggcat
     6901 caggtttgga tcatccattt gcccttcctg caggccatga caatgctgga ttcctttatg
     6961 accaaaattc aggtcgcttc ctgcttttca agacagaagc tccttgcttt gtggtctaca
     7021 cagcaaactt tgtggatgaa agtgtcatca taggaggtca gccaatgcta cagcacaatg
     7081 ggattgctct tgaagcgcaa gctttaccag atgccattca cagtgacctt aaaggccaag
     7141 tcattcttaa agctggtcaa accttcacca gtaagacacg ttatgaactt gttgtgaaat
     7201 aaaaaagctg gtcaaaaagt ctttaaatta aaataacgca tattgtcagg tgatgaaaag
     7261 tttgataata tgcgttttat tatgaaatga tttactttat cttatcctga aatggtgtta
     7321 tttttgagtt atctggcttg actttcttga tggaaattat ataatagttg taataacaat
     7381 aattacaacg agaaaagagg accatttatg acctatgaat acaagagtca catttatttg
     7441 gcagagacag ttttaaatgt aaaggatttg gccagtcaaa cagcctttta tcagcaagtt
     7501 attggtttag aaatcttatc tcaaactgag acagagtcca ttctaggcct tggtggaaaa
     7561 gtcttggtac agctgattca agcacaagag agcggagaag tgagggaaca ttatggtctt
     7621 taccatttgg ccattctctt gcccacacgc aaggctttgg ctgatgtctt gaaacatctg
     7681 acagatttgc aaattcctct tgtcggcggt gcagaccatg gttacagtga agctatttac
     7741 ttggaagatt tggagggaaa tggcattgaa ctctatcgag ataagccagt ttccacatgg
     7801 gatattcgag aagatggacg cattatcgga gtgacggagg ctcttgcggc gcaggacatt
     7861 tatgagttgg gggaaagagt agagcccttt atcctggcag aaggtacaag aatggggcat
     7921 attcatcttt ccgtcaagga tagtcgaaag tccagccagt tttatcaaaa ggtgttaggg
     7981 ctagagaata aattcagtgt gcctggcgct agttggattg cggctggaga ttaccatcat
     8041 catttagcag ttaacgaatg gggaggaaaa ggtctggctt cgcgtaagca aggcttacca
     8101 ggtttagcct actatgtcat cgaagttgca cataaagaag aactgttaac gattgcccaa
     8161 cgagcacaag aagttgacgt accaatcaaa tggatgacat cgagccaatt ggaaatcaca
     8221 gactcagatg gcatcgtgac ctgtattcgt ttagcaaggt aaaattagga atttctaata
     8281 agttataagt attgctagtt tgctttttga acggtctttg gtaacagaac tgcgtgagct
     8341 ggatgggtga gaattttgac aaagaagctg attagagata taatgaagaa aaatcgagca
     8401 aggaaaaaga aatgacaaat tcaaagtata ttacttgttt gaaacgttca gatggccagt
     8461 tgtgtgggat tcaaaagatg attgaaggag atcgtgactg tgctgatatt gtgacgcaac
     8521 tgacagcagt gagatctagt gtagagcgcg tgattgagat gataattacc gaaaatctta
     8581 ctgaatgtat caatcagcca ctagatgatt ctgaagctca aaaggaacgc ctagaaaagg
     8641 ctatccgata cttgattaaa cggaaataaa tgaatgtttt gagacgatat gtgcaaaggt
     8701 ctattaacta ttagaatagt ttaatgggag tcaaaagaag ttgtaaaaaa tctttaaaat
     8761 taaaaaaacg catagtatca ggtgttgaat gtactgaccc caaaaagtta gattttttct
     8821 gtctaacttt tggggtgcag ttcataagaa ccttggtaat atgcgtttta ttatggggag
     8881 atttatactt ttcgaaaatc tcttcaaacc aggtcagctt caccttgccg tagatatggt
     8941 tactgacttc gtcagttcta tccacaacct taaaacagtg ttttgagcag cctgcggcta
     9001 acttcctagt ttgcacttta attttcattg agtattactt catcttctca tgaaattgag
     9061 tttttgccca gctcctcttg attattctac ttgaccgggc caaacattca taccaccttc
     9121 tacgttggta acggtgaggc cttgggagct gagaaattgg caggtagatg cagagcggac
     9181 tcctccttga caaatgacat agtattcatg gtcaggtttg agctctttgt agccttgctc
     9241 taaggtactt aatggaagat ttttggcacc tggtgcatgt cctgcttgga attcatgtgc
     9301 ttcacggaca tcgataagtt ctagattttc attttggtat tttccataaa agtcagccat
     9361 gctgatacta gtttccatat tctactcctt ctgggttaac cattttgtat agtgaataag
     9421 cgccgtcaag gttttgggcg gtaaatcctg cttgcttgag gataggctct gcgatatagc
     9481 tgcgcaaacc actgtggcag ctgacgatgt aggcttgttt tttgtccagt tcatcagttt
     9541 cctccttgac actttgtcag tttttgatac aatagtacaa aattagagga ggcaggctat
     9601 gattcagaaa catgcgattc ctattttaga gtttgatgac aatcctcagg cggttatcat
     9661 gcccaatcac gaggggctgg acttgcagtt gccaaagaag tgtgtttatg catttttagg
     9721 tgaggagatt gaccgctatg caagggaagt aggggcggat tgtgtcggcg aattcgtttc
     9781 tgctaccaag acctatccag tctatgtcat caactacaag ggtgaggagg tctgtctggc
     9841 tcaggctcct gttggctccg ctccagcagc ccagtttatg gattggttga ttggctatgg
     9901 tgtggagcag attatctcta ctgggacctg tggtgtccta gctgatatag aggaaaatgc
     9961 ctttctagtc cctgttcgcg ctttgcgaga tgagggagcc agttaccact atgtggcacc
    10021 ttgtcgttat atggaaatgc agccagaggc tattgctgct attgaggaag ttttggaaga
    10081 cagagggatt ccttatgaag aagtcatgac ctggacgaca gacggttttt accgagaaac
    10141 ggctgaaaag gtggcttatc gtaaggaaga aggctgtgct gttgtggaga tggagtgttc
    10201 tgctcttgcg gcagtagctc aattgcgtgg ggttctctgg ggtgaattgt tgttcacagc
    10261 agattctcta gcggacttgg accagtacga cagtcgtgac tggggctcgg aagcttttaa
    10321 taaggcgcta gaactgagtt tagcaagtgt tcaccacctt tagttgtact ggcaaaggat
    10381 ttgttttatc ataaaatgtc tagctcatac ttttcaaaaa tatgtttaaa cgaggtcacc
    10441 ttcctcttgt cctaggcatg ttgaggttgg gaaaaatctt taaaatcaga aaaacgtatc
    10501 atatcaggtg atgaaaattt tgatactatg cgttttatgt tggtaagatt tagagtgaga
    10561 tgaaatgata ctcttcgaaa atctcttcaa accaggtcag cttcaccttg ccgtatagat
    10621 gttactgact tcgtcagttc tatttgcaac ctcaaaacag tgttttgagc aacctgtgac
    10681 tagctttcta atcgatgcct tggttctcat tgcctataat caaaaagaga aattttctcc
    10741 tgaaaagcat atagagtagc tggcgttaaa agctcctgtc ttgctttttt gacctatagt
    10801 cacatctatc aagtattgtt cttgcctaag ctatcaataa aaaggtggca ttttttaggc
    10861 ttggtgttag tagattttgc cttatcctat ctaagtcatt tcgagctttt tatggtacaa
    10921 tggaaacatg ttattcaaat tatctaagga aaaaatagag ctaggcttat ctcgtttatc
    10981 gccagcccgt cgtatttttt tgagttttgc cttggtcatt ttactaggct ctcttctttt
    11041 gagcttgccc tttgtccaag ttgaaagctc acgagcgact tattttgatc atcttttcac
    11101 tgctgtctct gcagtctgtg tgacaggtct ctcaaccctt ccagtagctc acacctataa
    11161 tatctggggc caaataatct gtttgctctt gattcagatc ggtggtctag ggctcatgac
    11221 ctttattggg gttttctata tccagagcaa gcaaaagctt agtcttcgta gccgtgcaac
    11281 tattcaggat agttttagtt atggagaaac tcgatctttt agaaagtttg tctattctat
    11341 ttttctcacg acctttttgg ttgagagctt gggagctatt ttgcttagtt ttcgccttat
    11401 tcctcaactt ggctggggac gtggtctttt tagttccatt tttctagcga tctcagcctt
    11461 ctgtaatgcc ggttttgata atttagggag cactagttta tttgcttttc agacagattt
    11521 actggtcaat ctggtgattg caggcttgat tattacaggc ggccttggtt ttatggtctg
    11581 gtttgatttg gctggtcatg taggaagaaa gaaaaaagga cgtctgcact ttcatacgaa
    11641 gcttgtacta ttattgacta taggtttgtt gttatttgga acggcaacta ctctctttct
    11701 tgagtggaac aatgctggaa cgattggcaa tctccctgtt gccgataagg ttttagttag
    11761 cttttttcaa acagtgacga tgcgaacagc tggcttttct acgatagatt atactcaggc
    11821 tcatcctgtg actcttttga tttatatctt acagatgttt ctaggtgggg cacctggagg
    11881 aacagctggg ggactcaaga ttacgacatt ttttgtcctc ttggtctttg cacgaagtga
    11941 gcttttaggc ttgcctcatg ccaatgttgc gagacgaacg attgcgccgc gaacggttca
    12001 aaaatccttt agtgtcttta ttatcttttt gatgagcttc ttgataggat tgattctgct
    12061 agggataaca gccaaaggca atcctccctt tatccacctc atatttgaaa ccatttcagc
    12121 tcttagtaca gttggtgtaa cggcaaatct gactcctgac cttgggaaat tggctctcag
    12181 tgttatcatg ccacttatgt ttatgggacg aattggtccc ttgaccttgt ttgttagctt
    12241 ggcagattac catccagaaa agaaagatat gattcactat atgaaagcag atattagtat
    12301 tggttaagaa aggaaagagc atgtcagatc gtacgattgg aattttgggc ttgggaattt
    12361 ttgggagcag tgtcctagct gccctagcca agcaggatat gaatattatc gctattgatg
    12421 accacgcaga gcgcatcaat cagtttgagc cagttttggc gcgtggagtg attggtgaca
    12481 tcacagatga agaattattg agatcagcag ggattgatac ctgcgatacc gttgtagtcg
    12541 cgacaggtga aaatctggag tcgagtgtgc ttgcggttat gcactgtaag agtttggggg
    12601 taccgactgt tattgctaag gtcaaaagtc agaccgctaa gaaagtgcta gaaaagattg
    12661 gagctgactc ggttatctcg ccagagtatg aaatggggca gtctctagca cagaccattc
    12721 ttttccataa tagtgttgat gtctttcagt tggataaaaa tgtgtctatc gtggagatga
    12781 aaattcctca gtcttgggca ggtcaaagtc tgagtaaatt agacctccgt ggcaaataca
    12841 atctgaatat tttgggtttc cgagagcagg aaaattcccc attggatgtt gaatttggac
    12901 cagatgacct cttgaaagca gatacctata ttttggcagt catcaacaac cagtatttgg
    12961 ataccctagt agcattgaat tcgtaaagag ggatgacccc tcttttttga tgcccaagat
    13021 ggcaaataga gacagaagcc ccttgtcttc tagtaaaagt tcttcaaagg ctggacttta
    13081 tggtaaaata gaaggaagtg acaagagaga gtaatactca atgaaaatca aagatcaaac
    13141 taggaaacta gctgcgggct gctcaaaaca ctgttttgag gttgtggata gaactgacga
    13201 agtcagctca aaacactgtt ttgaggttgt ggatagaact gacgaagtca gctcaaaaca
    13261 ctgttttgag gttgcagata gaactgacga agtcagtaac atctatacgg caaggcgacg
    13321 ttgacgcggt ttgaagagat tttcgaagag tataagaaaa aatcagtccc ctaaaggagc
    13381 agattatgaa gttattgtct atcgccattt ctagctataa tgcagcagcc tatcttcatt
    13441 actgtgtgga gtcgctagtg attggtggtg agcaagttgg gattttgatt atcaatgacg
    13501 ggtctcagga ccagactcag gaaatcgctg agtgtttagc tagcaagtat cctaatatcg
    13561 ttagagccat ctatcaggaa aataaaggcc atggcggtgt ggtcaatcgc ggcttggcag
    13621 aggcttctgg gcgctatttt aaagtagttg acagtgatga ctggtggatc ctcgtgccta
    13681 cttgaaaatt cttgaaacct tgcaggaact tgagagcaag ggtcaagaga tggatgtctt
    13741 tgtgaccaat tttgtctatg aaaaggaagg tcagtctcgt aagaagagta tgagttacga
    13801 gtcagtcttg cctgttcggc agatttttgg ctgggaccag gtcggaaatt tctccaaagg
    13861 ccagtatatc atgatgcact cgctgattta tcggacagat ttgttgcgtg ctagccagtt
    13921 ctaactgcct gaacatactt tttatgtcga taatctcttt gtctttacgc cccttcagca
    13981 ggtcaagacc atgtactatc tgcctgtcga tttctatcgt tacttgattg ggcgtgagga
    14041 ccagtctgtc aatgagcaag tgatgattaa gtgcattgac cagcaactca aggtcaatcg
    14101 actcttggta gaccagcttg atttgtccca agtgagtcat cccaaaatgc gagaatatct
    14161 gctgaatcat attgaaatca cgacggtgat ttccagtacc ctgctcaacc gatctgaaac
    14221 agcggagcat ctggcaaaaa aacgccaatt gtggacctat attcagcagg aaaatccaga
    14281 agtctttcag gctattcgta agaccatgtt gagccgtttg accaaacatt ctgtcttgcc
    14341 agatcgcaaa ctgtccaatg tcgtctatca aatcaccaaa tctgtttatg gatttaatta
    14401 atataagtgt tttataagag ggatttaaga aaaattttaa ctttttctta gtccttttta
    14461 atttcaggag attatactag agtcatcaaa taaagaaaga ctctaaggag aatcctatga
    14521 aattcaatcc aaatcaaaga tatactcgtt ggtctattcg ccgtctcagt gtcggtgttg
    14581 cctcagttgt tgtggctagt ggcttctttg tcctagttgg tcagccaagt tctgtacgtg
    14641 ccgatgtggt caatccgacc ccaggtcaag tcttacctga agagacatcg ggaacgaaag
    14701 agggtgactt atcagaaaaa ccaggagaca ccgttctcac tcaagcgaaa cctgagggcg
    14761 ttactggaaa tacgaattca cttccgacac ctacagaaag aactgaagtg agcgaggaaa
    14821 caaactcttc tagtctggat acactttttg aaaaagatga agaagctcaa aaaaatccag
    14881 agctaacaga tgtcttaaaa gaaactgtag atacagctga tgtggatggg acacaagcaa
    14941 gtccagcaga aactactcct gaacaagtaa aaggtggagt gaaagaaaat acaaaagaca
    15001 gcatcgatgt tcctgctgct tatcttgaaa aagctgaagg gaaaggtcct ttcactgccg
    15061 gtgtaaacca agtaattcct tatgaactat tcgctggtga tggtatgtta actcgtctat
    15121 tactaaaagc ttcggataat gctccttggt ctgacaatgg tactgctaaa aatcctgctt
    15181 tacctcctct tgaaggatta acaaaaggga aatacttcta tgaagtagac ttaaatggca
    15241 atactgttgg taaacaaggt caagctttaa ttgatcaact tcgagctaat ggtactcaaa
    15301 cttataaagc tactgttaaa gtttacggaa ataaagacgg taaagctgac ttgactaatc
    15361 tagttgctac taaaaatgta gacatcaaca tcaatggatt agttgctaaa gaaactgtc
//
LOCUS       DAVKAO010000040        14922 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKAO010000040 DAVKAO010000000
VERSION     DAVKAO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.040"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            4..828
                     /locus_tag="VWX16_001355"
     CDS             4..828
                     /locus_tag="VWX16_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9158695.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGYSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            850..1545
                     /locus_tag="VWX16_001356"
     CDS             850..1545
                     /locus_tag="VWX16_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9158696.1"
                     /translation="MHIEHLSHWSGHLNREMYLNRYGHGGIPVVVFASSGGSHNEYYD
                     FGMIDACASFIEEGLVQFFTLSSLDSESWLATWKNAHDQAEMHRAYERYVIEEAIPFI
                     KHKTGWFDGMMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFVGDYYNDDAI
                     YQNSPVDYIWNQNDGWFIDRYRQAEIVLCTGLGAWEQDGLPSFYKLKEAFDQKQIPAW
                     FAEWGHDVAHDWE"
     gene            1603..2010
                     /locus_tag="VWX16_001357"
     CDS             1603..2010
                     /locus_tag="VWX16_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoylphosphate synthase"
                     /protein_id="HEU9158697.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPINRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKQ"
     gene            2028..2747
                     /locus_tag="VWX16_001358"
     CDS             2028..2747
                     /locus_tag="VWX16_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9158698.1"
                     /translation="MIAKPDNGVGAAATFKLETEDDINHFKQEWDHSTLYFFEKFVTS
                     SEICTFDGLVNKDGKIVFSTTFDYAYTPLDLMIYKMDNSYYVLKDMVPKLRKYGEAIV
                     KEFGMKERFFHIEFFREGDDYITIEYNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGE
                     EFPASEFEPQYCLATSRRANAHYVYSEENLLAKYSQQFKVKKIMPEAFAELQGDYFYM
                     LTTPSRQEMDQMIADFGQRQE"
     gene            3255..4748
                     /locus_tag="VWX16_001359"
     CDS             3255..4748
                     /locus_tag="VWX16_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9158699.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            4761..5162
                     /locus_tag="VWX16_001360"
     CDS             4761..5162
                     /locus_tag="VWX16_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9158700.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            5222..5458
                     /locus_tag="VWX16_001361"
     CDS             5222..5458
                     /locus_tag="VWX16_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU9158701.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            5455..5868
                     /locus_tag="VWX16_001362"
     CDS             5455..5868
                     /locus_tag="VWX16_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU9158702.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            5986..6951
                     /locus_tag="VWX16_001363"
     CDS             5986..6951
                     /locus_tag="VWX16_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9158703.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            6985..8091
                     /locus_tag="VWX16_001364"
     CDS             6985..8091
                     /locus_tag="VWX16_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9158704.1"
                     /translation="MKKVKLGEVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSTEIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWRGRSAVLNQHIFKVVLDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNT
                     KNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLR
                     INSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLAL
                     QNEFADFVALVDKSQFACEIAIKVWRNSLKFSII"
     gene            8196..11472
                     /locus_tag="VWX16_001365"
                     /pseudo
     CDS             8196..11472
                     /locus_tag="VWX16_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            11743..11943
                     /locus_tag="VWX16_001366"
     CDS             11743..11943
                     /locus_tag="VWX16_001366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158705.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTRIEEIFEEYKSS"
     gene            complement(12037..12507)
                     /locus_tag="VWX16_001367"
     CDS             complement(12037..12507)
                     /locus_tag="VWX16_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9158706.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(12524..14797)
                     /locus_tag="VWX16_001368"
     CDS             complement(12524..14797)
                     /locus_tag="VWX16_001368"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9158707.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         4804 a         2488 c         3143 g         4487 t
ORIGIN      
        1 acaattaatc aatcctactt ttatctaaaa atgaaagaac acaaactcaa ggttccttat
       61 acaggtaagg agcgccgtgt acgtattctt cttcctaaag attatgagaa agatacagac
      121 cgttcctatc ctgttgtata ctttcatgac gggcaaaatg ttttttatag caaagagtct
      181 ttcattggat attcatggaa gattatccca gctatcaaac gaaatccgga tatcagtcgc
      241 atgattgtcg ttgctattga caatgatggt atggggcgaa tgaatgagta tgcggcttgg
      301 aagttccaag aatctcctat cccagagcag cagtttggtg gtaagggtgt ggagtatgct
      361 gagtttgtca tggaggtggt caagcctttt atcgatgaga cctatcgtac aaaagcagac
      421 tgccagcata cggctatgat tggttcctca ctaggaggca atattaccca gtttatcggt
      481 ttggaatacc aagaccaaat tggttgcttg ggcgtttttt catctgcaaa ctggctccac
      541 caagaagcct ttaaccgcta tatcgagtgc cagaaactat cgcctgacca gcgcatcttc
      601 atctatgtag gaacagaaga agcagatgat acagacaaga ccttgatgga tggcaatatc
      661 aaacaagcct atatcgactc gtcgctttgc tattaccatg atttgatagc agggggagta
      721 catctggata atcttgtgct aaaagttcag tctggtgcca tccatagtga aatcccttgg
      781 tcagaaaatc taccagattg tctgagattt tttgcagaaa aatggtaagt taagaaagga
      841 aaaaacgaaa tgcatattga acatcttagc cactggagtg gtcatcttaa ccgtgaaatg
      901 taccttaacc gttatggaca tggtgggatt ccagttgtgg tctttgcttc atcaggtggt
      961 agtcacaacg aatactatga ttttggcatg attgatgcct gtgcttcctt tatcgaggaa
     1021 ggccttgtcc agttctttac cctatctagt ttggatagtg agagttggtt ggctacttgg
     1081 aaaaatgctc atgaccaagc ggaaatgcac cgtgcctacg aacgttatgt gattgaggag
     1141 gccattcctt ttatcaagca caagacaggt tggtttgatg gcatgatgac gacaggttgc
     1201 tctatgggag cctatcatgc actcaatttc ttcctccagc atccagatgt ctttactaaa
     1261 gtgattgctc tcagtggtgt ttacgacgca cgtttctttg tcggtgatta ctacaacgat
     1321 gatgctattt accaaaactc gccagtagat tatatttgga accaaaacga tggctggttt
     1381 attgaccgtt accgtcaggc agagattgtg ctgtgtacgg ggcttggagc ctgggaacaa
     1441 gatggtttgc catcctttta caagctcaaa gaagcctttg accagaaaca aattccagcc
     1501 tggtttgctg aatggggaca tgatgtcgcc cacgactggg aatgatggcg taaacaaatg
     1561 ccttatttcc tcggtaatct ctatttataa aaggagttac ctatgaatta ccttgttatt
     1621 tctccctact atccacaaaa ctttcaacag tttaccatcg aactagctaa taaaggcatc
     1681 acagtcttgg gaattggtca agagtcttac gagcaattgg atgagccctt gcgcaatagc
     1741 ttgaccgagt attttcgtgt tgataatctt gagaacatag atgaagtcaa acgtgcagtg
     1801 gcttttctct tttataaaca tggtccaatt aaccgcatcg agtctcacaa tgaatactgg
     1861 cttgagctag acgcaacact cagagaacaa ttcaatgttt ttggtgccaa accagaggat
     1921 ctcaaaaaga cgaaatataa gtctgaaatg aagaaacttt tcaaaaaagc aggtgttcct
     1981 gtggtacctg gagctgttat caagcagtga aagaaatcgg tcttccaatg attgccaaac
     2041 ctgataatgg agtgggagca gccgcaacct ttaaacttga gacagaagac gatatcaatc
     2101 acttcaagca agaatgggac cattcaaccc tttatttctt tgaaaaattt gtcacttcca
     2161 gcgaaatctg tacctttgac gggctcgtga acaaggatgg aaagattgtt ttctcaacaa
     2221 cctttgacta cgcctataca ccgcttgatc tcatgattta taagatggac aattcctact
     2281 atgtgctcaa ggatatggtt cccaaactac gcaagtatgg ggaagcgatt gttaaggaat
     2341 ttggtatgaa agaacggttt ttccatattg agttcttccg tgagggggac gattatatta
     2401 ccattgagta caataaccgc cctgcaggtg gtttcaccat tgatgtttat aactttgctc
     2461 attccttgga cctctatcgt ggatatgcag ccattgtcgc aggagaagag ttcccagcgt
     2521 cagagtttga acctcagtat tgtttggcta catcacgtcg tgcaaatgct cactatgtct
     2581 attctgagga gaacttactt gccaaatata gccagcagtt caaggttaaa aaaatcatgc
     2641 cagaggcttt tgcggaactt caaggagatt acttttatat gctgacaact ccgagtcgac
     2701 aagaaatgga tcagatgatt gctgatttcg gacaacgtca agaataagaa ctatcggatt
     2761 aaggaaattc actcccttaa tccttttgtt ttgtctggta aaaaataaga gcatcctaac
     2821 aaggtagcta tcataaaact tgttcgataa ctatttgaag caggattagg tggctagaaa
     2881 ttgaatttta acatttcaat tgagtcatag tattgtgttt tcgtatcctt aaatcagcta
     2941 aaaggatcca tgacgacacc tatacgatat agttttcagg ataccaaaca agtctattaa
     3001 tactcaatga aaatcaaaga gcaaactagg aagctagccg caggttgctc aaaacactgt
     3061 tttgaggttg tggatagaac tgacagagtc agtatcatat acctacggca aggtgaagct
     3121 gacgtggttt gaagagattt tcgaagagta taaaatattc aggtgacgca tagatatagt
     3181 taattgaagc tttgtttgaa atctgataaa ataatgatat tactaagttt taaaaactaa
     3241 agaaaaggga agatatgatt acaggcgaat taaaaaataa aatcgatcag ctgtgggaaa
     3301 ttctttggac agaaggaaac gcaaatcctt taacaaatat tgaacagttg acttatctct
     3361 tatttatgaa agatttggat agtgtcgagc ttggacgtga aagtgatgct gaatttctag
     3421 ggattcctta tgagggagtt tttccaaaag ataaacctga ataccgttgg tcaactttta
     3481 aaaatatagg agatgctcag gaagtttatc gtttaatgac tcaggagatt tttccgttta
     3541 ttaaaaatct caagggggat acagatgata cagccttttc acgatatatg cgagaagcta
     3601 tttttcaaat aaataaacct gctacgcttc aaaaggcaat ttctatctta gatgtttttc
     3661 caactagggg attagatgta gattttgata atgacaaaca aagtattact gatatcggag
     3721 atatctatga atatctgtta tcaaaattgt cgaccgcagg taaaaatgga cagttccgta
     3781 cacctcgtca catcatcgat atgatggttg agttgatgca accgactatc aaagatatca
     3841 tctcagatcc cgctatgggt tctgctggct tcttagtatc tgctagccgt tacttaaagc
     3901 gtaagaaaga tgaatgggaa accaatacag ataatatcaa tcattttcat aatcagatgt
     3961 ttcatggaaa tgatacggat acgactatgt tgagacttgg ggcgatgaac atgatgctac
     4021 atggagtaga aaatccacaa atcagttacc ttgactcgct gtctcaagat aatgaagaag
     4081 ccgataaata tactttggtt ttagcaaatc ctccttttaa gggctcactt gactacaatt
     4141 caacctctaa tgaccttctt gcaaccgtaa aaaccaaaaa aacagaatta ctctttcttt
     4201 ctcttttctt gcgaacttta aaaccaggtg gacgagcagc agttatcgta cctgatggtg
     4261 tcctttttgg ttcgtctaaa gctcataaag gaattcgtca ggaaattgta gagaatcata
     4321 agcttgatgc tgtaatctca atgcctagtg gtgttttcaa gccttatgct ggagtttcaa
     4381 ctgccattct catctttaca aaaactggta atggtggtac tgacaaagtc tggttttacg
     4441 atatgaaagc ggatggttta agtttggatg ataagcgaca accgattagc gacaatgata
     4501 ttccagatat tatcgaacgc tttcatcatc ttgaaaaaga agcagaacgt cagagaacgg
     4561 atcaatcttt ctttgttcca gttgctgaga taaaggaaaa tgattatgat ttgtctatca
     4621 ataaatataa agagattgag tatgaaaaag ttgagtatga accaacagag gtcatcttaa
     4681 agaaaatcaa tgatttagaa aaagaaattc aagctggctt ggctgaatta gaaaaattac
     4741 tcaagtaggg aggtggctgt atgaaaaaag tgaagttggg ggaagtctta tctctaaaaa
     4801 aaggcaagaa agccactgta cttgctgaac aaacaactct aagccaacgt tatattcaaa
     4861 tagatgattt aagaaataat aataatttaa aattcactga aagtttaaat atgactgaag
     4921 cactcccaga tgatattctg atagcatggg atggagctaa tgcaggaaca gttggttatg
     4981 gattatcggg agctgttggt agtacaatta cggtcttaaa aaagaatgag cgatacaaag
     5041 aaaaaattat atcagattac ttgggagtct ttttggaaag taaatcacaa ttggcaatcc
     5101 aaaaatctct ggaagaactt gaaactttga agaaatctct gatgcaggag tattttggct
     5161 gatattctgc cattgtaatt acggtaatga tttgttataa tacttcaaag gaggaaatca
     5221 gatggtagta aaaacaagaa aacaaggaaa ttcaatcacc attacgattc caagtgaatt
     5281 taatattcca agtggtgtta aatacgaagc gaaattgtta ccaagtggtg agattatctt
     5341 tactcctgaa gaattggggc agcaggtttc ttatgtatct gatgatgcct ttgacttaaa
     5401 tttagataaa atatttgacg aatacgacga tgttttcaaa gctttggtgg aaaaatgaca
     5461 atctatctga cagaaaagca aattgaaaaa ataaatgctt tagcaattca acggtattct
     5521 ccaaatgaga aaattcaaac agttagttct tctgccttaa atatgattgt gaacttacca
     5581 gaacaatttg tctttgggaa gtctctttat ccaacaattt ttgataaagc aacgatacta
     5641 tttgtccaat tgataaagaa gcatgttttt gctaatgcta ataaaagaac tgctttcttc
     5701 gttttggtca aatttttaca attaaacggc tatcgttttt ctgtaacggt agaagaagca
     5761 gtaaaaatgt gtgtaaccat cgcagtagaa gctttaactg atgaaaaaat gacaagctac
     5821 tccaaatggg tttctgaaca ttctgttaga gaaaaggtca aaaagtaacc tagtatgctg
     5881 gatttgaatg agcacaagaa aataaatgaa cagacaatat tagaattctg taatgcagaa
     5941 actgatattg tctcttttta ttgatgaata agaaagtgag aaattatgga atcaaaagtt
     6001 acaattatca tgcaagaaat gttacctctt ttaaataatg aacaattact agcgttgaga
     6061 gagagtttag aacatcatct agtagacgga aaaaagcagc agaagtattc gaataataac
     6121 ctgttgcaac tatttattac cgccaagcag gtagagggct gtagctcaaa aacaattcgt
     6181 tattaccaga ggacgattga aaacttgttt aatgctatta aagagtctgt gacacaactc
     6241 acaacagatg atttaaggag ttatttagca aattaccagt ctgaaaagga ttgtagtaag
     6301 gcaaatttag acaatattag gcgtatattg tcttcttttt ttgcttggct tgagcaagag
     6361 gaatatatca ttaaaaatcc cattcgacgg ataaagaaaa ttaagactga gcaaaatgtg
     6421 aaggaaactt atactgatga acatttggaa attatgcgtg ataactgtga aaatttgaga
     6481 gatttggcaa taatagacct actagcatcg acaggtatgc gtgtagggga gcttgtacag
     6541 ttgaatcgtt cagatattga ttttgaaaac agagagtgtg ttgtctttgg taaaggaaag
     6601 aaggagagac cagtatattt tgacgctcgg acgaaaattc atttaagaaa ttatcttaac
     6661 gacagaaaag atagtcaccc tgctcttttt gtaacgctag ttggaaaagc ccagagactt
     6721 ggaattgctg gtgtagagat tcgcttaaga aagttaggag acaaactcgg catacaaaag
     6781 gttcacccac ataagttcag aagaacttta gcgactaagg caattgataa aggtatgcct
     6841 atcgaacaag tccaaaaact gctaggtcat agcaagattg acacaaccct ggcctatgcc
     6901 atggtcaatc aaaataatgt caagcattca caccaaaaat tcatctctta aaagcaaatc
     6961 ccgatattcc gaggaggtgg ttgcatgaag aaagtgaaat taggtgaagt ggctactttt
     7021 atcaatggct atgcttttaa acctcaagat tggtcatctg aagggaaaga gattatcaga
     7081 atacagaatc tgactaaaac ttcaacagag ataaattatt attcgggtac gattgataaa
     7141 aaatacattg ttgaagctgg tgatattctt atttcgtggt cgggtacatt aggggttttt
     7201 cagtggcgtg gaagatccgc agtgttaaat cagcacattt ttaaagtggt tttggataag
     7261 atagatatag ataaatcgta ttttaaatat gttgttgaaa aaggtttaca agatgctgta
     7321 aaacatactc atggttccac aatgaaacat ttaactaaaa aatattttga taatattata
     7381 gttccatata caaatttggg agagcaacaa agaattgcaa gtgagctgga tttgttaagc
     7441 aagctgattt taaggagaca agaacaactt gaagagctta atctacttgt aaaatcccga
     7501 tttaacgaga tgtttgggga aaataaaata tttgaaagca ttgataactt atttgatatt
     7561 atagatggtg ataggggcaa aaattatcct aaatcagatg agttgtttag tgaggagtac
     7621 tgtttatttt taaatacaaa gaatgttact aaaaacggat tttcattcga tacaaagcaa
     7681 tttatcacta aaacaaagga taaattactt cgaaaaggca aacttgagcg ttatgatata
     7741 gtcttgacaa caagaggtac tgttggaaat gtagcgtact acgatgaatt aataaaatat
     7801 aaacatttac gtataaattc aggtatggta atattacgtc ccaagacacc aaatctaaat
     7861 cagaaattta ttattcatgt tttaaggaat aataattata gtcgagtgat atcaggaagt
     7921 gctcagcctc agttaccaat tacaaaatta aaaaaaatac ttctccccct tcccccacta
     7981 gccctccaaa atgagttcgc agactttgta gccctggtcg acaaatcaca atttgcttgt
     8041 gagatagcta taaaagtgtg gagaaatagc ttgaaattta gtataatata gctaaactat
     8101 ttgtttaaag tgagaaaaaa atgggaaatt ttagctttct tttaaaaaat gacgaatatg
     8161 aatctttttc aaaaccttga attgaagctg agaatatgat tgctacatca actgtggcta
     8221 ctgcctttat ggcgcgtcgt gctttagagc aggctgtcca ttggatatat agtcacgatt
     8281 catatttaga agctccctat cgtgctactc tatcttcttt agtatgggat gatgatttta
     8341 gggatatcgt agattctgaa ctccacaagc agatagttct gttgattcgg tggggaaacc
     8401 atgctgctca tggtggtgaa attaaggaac gagaagcgat tttagctttg catcatttgt
     8461 atcagtttgt taattttatc gattattgtt acagcaatga gtttgtggag cgttattttg
     8521 atgagaagtg cttaccactt tcagcaaaca tcaaataccg agaaactcca caatctatga
     8581 taaagttaca agacagttta ccagaactgc ctgattttca tgaacagatg gctgctcagt
     8641 ccgtagaagt tcaagagact tatactgaaa aacgtgagac tgcagcgcaa cggcaagatg
     8701 tgcctttcca tattgatcaa ttgtctgagg cagagacaag aaagctcttt attgatatcg
     8761 atctccgttt agcaggatgg atatttgaag aaaactgtcg tgttgagata gccgttgatg
     8821 gtctcaagca cggttcagga attggttact gtgactatgt actttatggt aaaaatggga
     8881 aaattttagc gattgtagag gctaaaaaag cctctgtcaa tccagaagta ggggaagtac
     8941 aggtcaaaga atatgctgaa gctttggaga aacatatcgg ctatcagcca atttgcttta
     9001 ttacaaatgg gttgaagcac tatatacttg atggtccgaa ccgccgccag attgcaggct
     9061 tttactctca agaagaattg caattagtga tggatagacg tcatcttcaa aaaccgcttg
     9121 aggatatttc tagtaaaatt agggacgata tttccgggcg tcactaccaa aaacatgcca
     9181 ttgcaagcgt ttgtgaagct ttctctaatc atcgtagaca ggcacttttg gttatggcaa
     9241 ctggggcggg gaaaactcgt acagcagttt ctctagttga tatcttatca cgtcataact
     9301 gggtaaaaaa cgttctcttc ttagccgata gaacttcctt ggttaagcaa gcctatgatt
     9361 cgtttagaaa attactccca gatctttccg tttgtaactt cttagaagat aaagaaggag
     9421 ctcaatcaag tcgtatggtc ttttcaactt atccgaccat gattggagcg attagtggtc
     9481 aagaagaagt aaatcaacgc ccttttactg ttgggcattt tgaccttatc ataattgacg
     9541 aatctcaccg ttctatttat cagaaataca agtccatttt tgattatttt gatgcaagaa
     9601 ttgtaggctt aacagctact ccgcgtcaag atttagataa aaacacctat ggattcttta
     9661 atttggagaa tggggttcca acatatgcat atgatttgaa agaggctgtt aaagacggat
     9721 atttagtagc ctatcattct atcgaaatca aactgaaact acctacggat ggtctacatt
     9781 atgatgattt gtccgaagaa gaaaaggaac attttgatag caaatttgaa gacgatagct
     9841 gtgaaaaaga tattgatggg agtgtattta attcctttat tttcaataaa agtacagtag
     9901 aaattgtttt aaatgaactc atgacaagag gaattcagac agcctcgggt gatgaaattg
     9961 gtaaaactat tatttttgct aaaaatcatg atcatgcgga atatatcaga ggtattttta
    10021 acaaccgcta tcctgaaaaa gggagcgact atgctcaggt gattgattat agtattaagc
    10081 attatcagac cttgattgat gattttaaaa ttaaggagaa gtatcctcaa attgcgattt
    10141 ctgtcgatat gttagataca ggtattgatg taccagaggt tgttaattta gtcttcttca
    10201 agaaagtacg ctctaaaact aagttttggc agatgattgg tcgaggaacc cgtctatgta
    10261 aagatttatt tggacctgag caggataagg aaaacttctt ggtatttgat tatggggata
    10321 attttgatta ttttcgtgca gatccaagag atggagaagg gtcgtcacat tgtttcgttg
    10381 actcagcgtt tatttaatat caaagtggac ttgattcgag aacttcaggg actccaatac
    10441 caagaagatc agtttgcgag agcataccgt cagcagcttg tctcggaact tcaaggtcgt
    10501 atagagagct taaatgagtt ggacttcagg gttcgtatgg ttttagatac agtttatagc
    10561 tataggaaat tggaaagttg gcagaatcta actgctgtta caagtgaaac cattcaaaaa
    10621 aatctctctc cgcttttatt tgatgaagat aaagaagatg agatggcgag gagatttgat
    10681 ttgtggttgc ttcatattca gttggggcaa ctgacagcta aatcttccac tgttcatatt
    10741 tcccaagtga tgaagacggc tagagctctt tctgctattg gcaatatccc gcaggttttt
    10801 gagcaggctg aaattatcag gaaagtacag gagcctgaat tttggaaaga agttaacttg
    10861 tctgatttgg aaaaaattcg tcttgctatt cgagatttat tacagttttt ggataaaaca
    10921 gaccgtaaac cctactatgt taactttgaa gatcgtatac tctccactgt tcacgagacc
    10981 acagcatttt tgcaggtcaa cgatcttcgg tcttacaatg aaaaagttga gcattatttg
    11041 aaaactcatc tggatgagga gtccatttct aagctatacc ataataaaaa gttgacatct
    11101 gatgatatgc ttgcacttga aaaattgctc tgggaaaaat taggtagtaa agcagactac
    11161 caaagtcatt atgaaaataa ggcaattccg agattggttc gtgagattat tggcttagat
    11221 agagagtctg ccaatcgtat tttttctaaa tttttgtcgg atgagaatct taatgccagg
    11281 cagatttcat ttgtaaaatt gattgtagac tacattgtag aaaatggttt tttagagacg
    11341 aaagtgttaa cgcaagagcc gtttaaatct tatggttctg ttcaactact cttccaacac
    11401 caactaccag tacttcgtaa tattgttcaa atcattgaac ttatcaataa tcgagctgga
    11461 gaagcggctt aaattctaaa gtgattgcca tgctgagact catttaaaat taaaaagagt
    11521 agaaatttat gctatatatg agaagtttta ttaggaagaa tgtcatcgtt ttcctagaat
    11581 acagtatcag ttgttaagtg gttgataaat ttcaaagtag atacttgtac cacgatgttt
    11641 gttgatcgag ttattaacaa aagagctact ttgattttaa agaaatagaa aacaaaaagc
    11701 tgagcaagaa ttcaattgca ggagaaaatg aaataatact caatgaaaat caaagagcaa
    11761 actaggaaac tagctgcagg ctgctcaaaa cactgttttg aggttgtaga taagactgac
    11821 gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt cagtaaccat
    11881 acctacggca aggcgacgtt gacgcggatt gaagagattt tcgaagagta taaatcttcc
    11941 taggatagag caaaacgcat agtatcaagg gttttctgat gttaaagact ttctaccagg
    12001 ttttttaaaa gcataattgt tagttgtagt catttattat tcttcaaaga aaaatggtgg
    12061 ggcgaatttt ttcagttctt caaagcactt ttgagcagta tctgcatctt cacagatgat
    12121 aagacagaca tcattaccac aaagggtagc gatagcgtca gggaagctca aagtatcaat
    12181 gatagaacca aaggattgag ccagtccagg aagggttttt agtaggactt ggtgttgaac
    12241 tgggcgcatc cagacaaggg cgtcttccat gtagagttcg agacgttttt cccattttga
    12301 gatggaacca ttgttaagaa cataataagc gctatcttct tcgcggactt ttgataggtt
    12361 catatttttg atgtcgcgtg agagggttgc ctgggttact tgaatgtcgt tctcagcaag
    12421 aagggcttgc aactcagcct gtgtatgaat cttgtttttt gtgataagag cgcgtataag
    12481 ttggtggcgg tgttctgatt tattcataat aatgtaactc cttttagcaa ggtaaggtaa
    12541 gcatggactg agcgaggtcg acagtcaagt ggtagtctgt attgtcacgg atggtgattt
    12601 caaagtcagt agtatagagg actaaacgga gagtgtctcc ttcttttagc ttgtaaatag
    12661 ttggctgcag ttcaaattga acgtccatcc attcatctgc agtaatatcc tctactaaca
    12721 gtaaatcatt tctattttgt aaattaaggt aaccttttgt cacgactcgt tgtgcctctg
    12781 gtctaaatgg caattcacag agattttcca acatgtgata gcgaccgtta tcaatggttc
    12841 tagcacttaa aatagctgga taaggttgta ggtatttctt ttgcccaaat tctagcagtt
    12901 gggcagataa gagccccttg tttgtactgg atttgatacg aagattgagc tgagcgcgac
    12961 cgtttaggtg gagatcttta gtcacaggaa ggttaatagt aatctgattg gctttccctt
    13021 gatagagctc tgtattgaag gtttggtatg tcttaccata gcgctcaaaa tccttatctg
    13081 ggtactggtt ttgaatagct tgctcttctt gaccaagtga gaaggtttca cagttttctt
    13141 gcccaccgaa gttatcaagt gataaccaag tctgtggagc tgtattgtcc tgccagataa
    13201 cagtaggaag ttgaaagtct gtttcctgtc ctagtaattt cttggtcaat aaggcattta
    13261 tggactcacg gaagtcaatt gattgccaat tgttcatgta aacatgagca ccattatgga
    13321 aaaagagatg cttgtgtata tgagtaggaa gagcatggaa catctggtaa acatgaagtg
    13381 gtttgacatt ccaatcctga gaaccatgag taaagacaac ctctgccttt actttatggg
    13441 cattgagcag ataattgcgg tcatgccaaa actgattgta gtccccagtt tttcggtcta
    13501 gctgagcttt cactttttct aagtcagctt ggtgagcttc attgccacgg atatagtcgc
    13561 cagctaagag attacgagaa taggttaact cagcaaggga gtcaaagtcc tcacctggat
    13621 aaccacctgg gctagtcacc agaccgtttt cacggtagta gttgtaccat gatgaaattc
    13681 ctgcctcggc aatgataact tctaaaccat cgactcctgt agtcgcaaga ccattggaca
    13741 tggtacctag ataggaaagt cctgttgtag caacttttcc gtttgaccaa tcagccttga
    13801 cttgacgctg gcgcgtgtga tcagtaaagg cacggcaacg gccgttaagc caatcgatga
    13861 catttttata agcctcgatt tgctggtagt ctccattagt catgaaacct gtcgagtctt
    13921 tggtaccaac acctgagaca tagagattgg caaagcctct cggaaggaag tagtcgttta
    13981 gtgtatagct agagttgatg tgagttagct tttcctcagc ctctgctata agctcagctt
    14041 taccttgggg ttggacgaga tttagttgag gtttctctag ctcaatcttg tgaggaagct
    14101 taacctcaag ctcgccctcc atcttgtaga gagccttgtc actagccttg tcattggttc
    14161 cctgatgata agggctggct gtcatgatgg cagggatttt tccatcaaaa cgagggcgaa
    14221 taatgctaac ctttactagg tctgatagcc ctttttggtc agtatcgaca cgagactcaa
    14281 cgtaaacgac ttcacgaatg acatcctggt tagaaaaagt agccaaactc ttgccgttaa
    14341 agtagtggta gtcattatcc tccggaataa gaccatcact aacaagttgg tcgataagag
    14401 tatttccttt tttggtgcga gtattgagta actgatagag attttcaatc aagtcaccat
    14461 atataatggg aaatccagtt tctttacgaa aaacgtcact atcttcgaag tcaaccaaat
    14521 aagaaaagcc taaaagttga aaagcaacag tataaaaaat atctgctgtc agttcatctt
    14581 ctgattgaaa aaatgtcagc aggtctgttt ttttatcagc tgctaggata gaaagtgggt
    14641 agttggtgtc ttgataagtg aaaaagaaac gacgtaaaaa ggtttcaagt gagtctttgt
    14701 gattggctgt attttgtaaa tcaaagccac atttttttag ttcagataag acattttctt
    14761 ttggaaaatt gatataacta tattgattaa aacgcataga acctccatat agaatgacag
    14821 ttaaggttat tatatcaaaa aaaaagcaga aagggaattg ttaacttcaa aaggaaataa
    14881 tccaataaaa atgaataaag tactaaattc aatatagaga ac
//
LOCUS       DAVKAO010000041        14163 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKAO010000041 DAVKAO010000000
VERSION     DAVKAO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.041"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            376..633
                     /locus_tag="VWX16_001369"
     CDS             376..633
                     /locus_tag="VWX16_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEU9158708.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            complement(758..2197)
                     /locus_tag="VWX16_001370"
     CDS             complement(758..2197)
                     /locus_tag="VWX16_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9158709.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(2201..3550)
                     /gene="trkA"
                     /locus_tag="VWX16_001371"
     CDS             complement(2201..3550)
                     /gene="trkA"
                     /locus_tag="VWX16_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9158710.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            3739..4587
                     /locus_tag="VWX16_001372"
     CDS             3739..4587
                     /locus_tag="VWX16_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9158711.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            4602..5150
                     /locus_tag="VWX16_001373"
     CDS             4602..5150
                     /locus_tag="VWX16_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9158712.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINVPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            5157..5414
                     /locus_tag="VWX16_001374"
     CDS             5157..5414
                     /locus_tag="VWX16_001374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158713.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            5516..7198
                     /locus_tag="VWX16_001375"
     CDS             5516..7198
                     /locus_tag="VWX16_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158714.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            7183..8013
                     /locus_tag="VWX16_001376"
     CDS             7183..8013
                     /locus_tag="VWX16_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9158715.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            8206..8709
                     /locus_tag="VWX16_001377"
     CDS             8206..8709
                     /locus_tag="VWX16_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9158716.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      8763..8981
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9146..9709
                     /locus_tag="VWX16_001378"
     CDS             9146..9709
                     /locus_tag="VWX16_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9158717.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            9723..10349
                     /locus_tag="VWX16_001379"
     CDS             9723..10349
                     /locus_tag="VWX16_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9158718.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            10377..10766
                     /locus_tag="VWX16_001380"
     CDS             10377..10766
                     /locus_tag="VWX16_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9158719.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            10771..11220
                     /locus_tag="VWX16_001381"
     CDS             10771..11220
                     /locus_tag="VWX16_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU9158720.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKENGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKKIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            11348..11935
                     /locus_tag="VWX16_001382"
     CDS             11348..11935
                     /locus_tag="VWX16_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9158721.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            12325..13932
                     /locus_tag="VWX16_001383"
     CDS             12325..13932
                     /locus_tag="VWX16_001383"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9158722.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         4181 a         2607 c         3209 g         4166 t
ORIGIN      
        1 taaaaacaaa aatgagaatt tcatataaag tttattctag gagtatgtat cttgaataaa
       61 aatcgttatt taagttcagg gttatgatga atgtttttat aataataaaa tgcagctaga
      121 agaaaagtta ttttaatata aatcactcaa catagagaga aggcggtttc ctatatggaa
      181 gtcgtcttgt tttcatattg tctaaatacg agatatctac tacaggttgt agatattgca
      241 agctcaaatg atattatttt agaggaggat acaagtgaaa taatgcctgg tgagatagat
      301 gtaataaaaa gtaaggagtt gaaagtactg gttctttgtg cagggtctgg aacaagtgcg
      361 taattagcca atgcaattaa cgagggcgct caattagcag aagttagagt gattgcgaat
      421 tcaggagcgt acggagctca ttatgatatt atgggtgttt atgatttaat tattctggct
      481 ccacaagttc ggagttatta tagagagatg aaggtggatg cagaaagatt aggtattcag
      541 atagttgcta ccagaggaat ggaatatatt catttaacaa agaatccaag taaagcctta
      601 caatttgtat tggagcatta ccaagctgtg tagtaagttt ttccatcttt tatttgagta
      661 agaactttgc ttacagacag gcttggattt aaaaacgttc ccccttttta atataaaaat
      721 ccctctttca caattgtaaa aagagggatt ttgtatttta tctcttagac caagttcttt
      781 tcataaagag aaggaggatt gggtaaatct ccaagcgccc tgcaatcatt gcaaaggaga
      841 ggagaatttt tgagatggga ctaaagattg agaaactaga agtggttcct agaataggcc
      901 cgatattatt gaaacagcta aagacagcgc tggtcacgac cagaaaatca ttgctatcta
      961 ggctgacaat aaagataagc gctagcaaaa tcatagcata gatgacaaag tacttgagaa
     1021 tcttatgctg ggtatctttg tcaatcacca ttttattaac atggagggtc aaaacacggt
     1081 ggggcgatag gattgacaaa atttggtttt tggcaatttt tgaaaggatg aggcctcgaa
     1141 taatcttgag tccacctgca gttgatccag cagagccacc gattgccatg aggaaaagga
     1201 ggataaactg ggagaagagg ggccagttgg taatatctcc atatccaaaa ccagttgttg
     1261 taatgatgtt ggaaacctgg aagaaggtca tttcaaagct ctttgaaaac cctgggtaga
     1321 ggtagagagt attgaggcta atcaagcctg tagaaaccag cacgatgacc aagtaagccc
     1381 gaagctcttc atctccaaag aaggccttaa cccgacggag cataaggtag tagtagagat
     1441 tgaaatttac cccaaaaacc aaaactccta tactaaccag ataggtaatc agtgagctac
     1501 catagtgggc aattccatcg ttatagacgg taaatcctcc agttcccgct gtccccatag
     1561 caatgacaaa actatcgtag agaggcatac cggctagata atagatgatg acaaagaggg
     1621 aaaagagggc tatgtaaagg agatagagga tctgggcagt gttttttaat ttggatacaa
     1681 ccttaccaaa gacaggacca ggaacctcag ccttcattac ttctaggtga ctatttttgg
     1741 cattgtccat aatagcaagt gcaaaaacaa gcactcccat ccctccaatc aagtgggtaa
     1801 aacttcgcca gaagaggagg gaacggctga gaaccgaaat gtcgttcaaa atacttgctc
     1861 cagtagttgt aaacccagaa ctaatttcaa aaaaggcatc aataaggcta gggatttgcc
     1921 cagaaaagac aaaggggaga ccaccaaaga aagaccaaag gatccaacag agggcaacga
     1981 tcaagactcc ctccttggca taaatccgtt gattttttgg cttctgtaaa ctccctgaac
     2041 cgcctaacaa tacgagaatc cctatggtcg aaaagagggc tgtaaagact tggctcgatt
     2101 cacggtaata gacagcgata gccacaggaa ccaagaggag aacagcttca atcaaaagta
     2161 actttgaaag gaggtaacga atcatacttt tattcatttc ttacctcgca atcaagtcat
     2221 aaatcttggt gatgtttggc aacaaggttg ttactaggag cttgtctcca acttccaaca
     2281 tatcctcccc agttgggaaa atagtcttgc cctttcgaat aatggctgca ataagaaccc
     2341 cttttttcaa tttcagttga gaaagaggtt tggcagtcat tttattggct tccttgatat
     2401 ggaattgcag ggtttcgatt tggccattgg ctagatggtg catggcttga aggtctgaat
     2461 actgggcatt aactcgacca cgaataaagt gcataatcgt atctacagcg atgcttttag
     2521 gtgtgatgat acttgaaaaa tcaggcgcat tgataatctc gaggagactg gtacgattga
     2581 ccttagtaat atttttctgt acacctaccc tgtcaaggaa catagatgta atcagatttt
     2641 cctcatcgac tcctgttaga gtcgcaacgg catcatagtg ttgagcactt tcttccagca
     2701 ggatatcttt tgcggttcca tctccttgaa cgatgtagag atttgggaat ttctcgctaa
     2761 agaagctggc gatttcagga ttgatttcaa tgacttttgt atcgatacga ctatctttga
     2821 gaataccaag tagataatag gcaattctac ctgccccaac gatgagaagg ctcttcacgg
     2881 cgcgtgattt aaaataatta tggaagagta tcatatcgac acggttacca gtgacaaaga
     2941 ttctatcttt atcctgtaca gtcatgtcac cgcttggaat gataatttga tgatccctct
     3001 ctatcgcaca gacaatgaca ttaccaaatt ttttacgaaa atcagaaatg ggcatttggc
     3061 aaagaccgct ggtggacttg acgacaaatt ccatgaggct aacgcgtcca ccagcaaagc
     3121 gttcgacaga cagggcgttg gggaagtcaa tgatattcgc gatagcgcgg gcagccaaga
     3181 gctcaggatt aacgataaga gaaaaaccga gaatattctt ttccttgaaa taagagttag
     3241 aatattcagg gttccgcacc cgaacgatag tttctttagc tcccattttc ttggctagaa
     3301 ctgctgcaat catgttgact tcatcgtgct cagtcagggc gataaagata tcacaatctt
     3361 ggacgctggc ttgctcaaga atggcaaaat cggccccgtt accaaggata ccaatgatat
     3421 caaagcgact gacaatatga ttgagaacag cttcgtcttg ctcaatcagc aaaacatcat
     3481 gcttttctgc aaccaaggag cgacagaggg caaaaccaac ttttccccct ccgacaagga
     3541 taattttcat aataaaacct actttttcat gatgtaacta tcataccctt tttcaagaaa
     3601 aaatgcacct actagctaat aaccagagtt tttagtagga aatttgctat aagttaaaac
     3661 tataccctaa ccaattgaaa tagctattag cgactttctc tgaaatatgg tatgataaag
     3721 gatatacaag gagataaaat gaataataat ttactggtat tacaatcaga ctttggtctg
     3781 gttgatggtg cggtatcggc tatgattgga gtggctttag aagagtctcc aaccttaaaa
     3841 atccatcact tgacgcacga tatcacgcct tataatattt ttgaggggag ctatcgtctc
     3901 tttcagacgg tggattactg gcctgaggga acgacgtttg tatcggttgt cgatccaggt
     3961 gtcggttcga aacgtaagag tgtagttgcc aagactgcaa aaaatcaata cattgtcacg
     4021 ccagataatg ggacgctttc ctttatcaag aaacacgttg gcattgtagc cattcgtgag
     4081 atttctgagg tggccaatag gcgtcaaaac acagagcatt cttatacctt ccacggtcgt
     4141 gatgtctatg cctatactgg tgctaaactg gccagtggtc acattacttt tgaggaagta
     4201 gggccagagc tcagtgtgga acagattgta gagcttccag tcgtagcgac catcatagaa
     4261 gatcatctgg tgaagggagc cattgatatt ctggatgtgc gtttcggttc gctttggacc
     4321 tctatcacac gggaagaatt ttacaagttg gaaccagaat ttggtgatcg ttttgaagtg
     4381 accatctatc atgctgatat gctggtctat caaaatcagg ttgtctatgg caaatcattt
     4441 gcagatgtga gaattgggca acccattctt tacatcaact ctctctatcg tttaggtttg
     4501 gctatcaacc aaggttcctt tgccaaggcc tataatgtag gtgtcggttc atcttggacc
     4561 attgaaataa agaaaataga aggataaaat aggagaatag tatggaaatc aaatttacaa
     4621 ttaaacaagt tgttgctgtc ggaattggcg ctgccctctt tgtcgtcatc gggatgatca
     4681 acgttccaac ccctgttcca aatacaagca tccagcttca gtatgcggta caagcgctac
     4741 tttctattat ttttggaccg attatcggtt tgcttgttgg gttgattggt catgcaatca
     4801 aggactctct tgttggttat ggtctatggt ggacttggat tatcgctagc ggactctttg
     4861 gtttagttgt gggacttttt agaaagtacg ttcgcgtgat caatggtgtt tttgactgga
     4921 aagatattct tatttttaac ctcattcaac tacttgcaaa tgcccttgtt tggggtgtct
     4981 tggcaccact tggagatgtt gtgatttatc aagaagcggc agaaaaagta tttgcacaag
     5041 gaattgttgc gggaattgcc aatggtgtat ctgtagctat tgcaggaact cttctcttac
     5101 ttgcctatgc aggaacccaa actcgtgcag gaagtttgaa aaaggactaa tactcaatga
     5161 aaatcaaaga gcaaactagg aagttagccg caggttgctc aaaacagtgt tttgaaattg
     5221 tggatagaac tgacgaagtc agctcaaaac atggttttga ggttgtagat gaaactgacg
     5281 aagtcagtaa ccatacctac ggcaaggcga aactgacgtg gtttgaagag attttcgaag
     5341 agtataaaat gatggggaag gctgggcaac tagtcttttt cgatgtttat agattagtta
     5401 ggcaagtaag ctaggatagg aatctttttg gtgctaagat tttaagagaa gagactgcaa
     5461 gaagggcttg atttatgata aaatagaagt ctaagaagcg aatgaagaga gtaagatgaa
     5521 agaagctata attgagtgga aggatttctc tttccggtat gaaacacaac aagaaccgac
     5581 cttgcaaggg atagacttga ccatttacaa gggagagaaa gtcttaattg ttggaccatc
     5641 tggatcaggt aaatctacct tgggtcagtg tttgaatggg attattccca atatttacaa
     5701 gggtcagaca tatggagaat ttttgataaa gggtcaaaca gcctttgata tgagcatcta
     5761 tgataagtct catctggtta gcacagtttt gcaggataca gatgggcagt ttattggctt
     5821 gtctgtggca gaagatttgg cgtttgctct ggaaaatgat gtgacagccc tagatgagat
     5881 gaaaggtcgt gtttataaat gggctgaaaa gctggacctt cttcctttac tggatcagcg
     5941 tcctcaggat ttgtcaggtg gacaaaagca gcgagtcagt ctggctggtg tcttgattga
     6001 tgaaagtccg attctcttgt ttgatgagcc actcgccaat ctagatccca agtcaggtca
     6061 ggatattatc gaattgattg accagattca taaggaagag gggacgacga ctcttattat
     6121 cgagcaccgt ttggaggatg ttctgcatcg ccctgtggat cggattgtct tgataaacga
     6181 tggtcgtatc ctttttaatg ggagccctga ccagttgctt gcgactgatt tattgactca
     6241 aaatggaatt cgagaacccc tttatctaac gactctccgt caattaggtg tggacttagt
     6301 caaggaagaa cagttagcga atctggataa catgtctatc tcaaaaggtc aggttcagct
     6361 gcagaatgaa ctggtaaaag aaactccaga attgcagtca ctctttaaat tagaggacgt
     6421 gtctttctct tatgatgata gaccgatttt aaaatcccta catttagata ttaaaaaggg
     6481 tgaaaagatt gctattgtcg gaaaaaatgg agcagggaaa tcaactctag ccaaggctat
     6541 aagtagcttt attcagacgg aaggacgcta tctttgggaa aaacaggata taaaaggcga
     6601 ttctgttgca gagcgggcgg aacgagtagg ctatgtgcta caaaatccta atcaaatgat
     6661 ttcaaccaat atgatttttg atgaggtggc tctagggctc cgtttgcgag gtgtggatga
     6721 gaaggaaatt gaaacgagag tctatgaaac cttgaaaatc tgtggacttt atgaattccg
     6781 taattggcct atttctgccc tgtcatttgg tcagaaaaaa cgtgtcacca ttgcttcaat
     6841 tttggtctta ggagctgaaa ttattctcct agatgaaccg actgcaggtc aagatcagaa
     6901 gaactatact gagattatgg aatttctcga agagttacat cgaaaagggc ataccattgt
     6961 catgattacc catgatatgc aattgatgct ggattattca gaccgagtcc ttgtcatggt
     7021 ggatggagaa ttgattgccg atactgttcc agccagtctg ttgagcgatc ctgagctgtt
     7081 agtaaaagcc aatctaaaag aaacctccat ctttaacttg gctaagaaac tagatgtgga
     7141 tccactggat ttaacggcat tttacaaaga aaggagagag ggatgcaagc taaattaatc
     7201 ggttaccagc atagagatac tgtgattcat cgcttgtcag gagctgggaa acttctcttt
     7261 ttcattctgg tatcattggc ggccatgatt agctatgata ccagaatgct cttgctgatt
     7321 gccatctttt cagtctttct cctctattta tcagaaattc gctttaaaga tgtttccttt
     7381 gtagccgttt ttgcgacggt atttgccgtt ttaaacgttt tgatggtcta tctcttttct
     7441 cccgagtatg gggttggact ttatggagag agaagtgtga tttggcaggg aatcggtgtc
     7501 tacactctaa ccagccagga gctcttttat ctgctaaatt tggccattaa gtatctttgc
     7561 accattcctc tggctattat ctttttgatg acaacccatc ctagtcagtt tgcttccagt
     7621 ttaaatcaaa ttggtgtgcc ctataagatt gcttattctg tcagcctgac cttgcgctat
     7681 attccagatt tgcaggaaga attctttact atcaagatgt ctcaggaggc gcgtgggatg
     7741 gaattatcca agaaagcttc tcttatgcaa cgaatcaaag gcaatctgct cattattacg
     7801 cccttgattt ttagctcgct agaacgcatt gataccattg cgactgccat ggagcttcgc
     7861 cgctttggga aagaaaaaaa acgcacatgg tatagttatc aggccttgaa aaaaggagac
     7921 tatattacct tgctcttggc aaccttgttt ttagtagtta gtttactact tatcttgcag
     7981 aatcagggac gattttacaa cccttggaaa tagcttgaaa aaattgaaaa aatcaagtca
     8041 tttctattga caatgattct gaaagtgtta tactaagaaa gtagtttcgc tgatttactt
     8101 caaacctgtt gtgaggtaag ttaacgatgc cttaaccacg ctgtttgctg agcttgactc
     8161 cgggcagtgt ggctattttt ttgcaatggt gaaaggaagc aagtcatgac aaatcacatt
     8221 gtattatttg aacctcaaat tccacaaaat acgggcaata tcgcgcgtac ttgcgctgcg
     8281 accaattctc ccctccacat catcaagcct atgggctttc ctattgatga ccgcaagatg
     8341 aagcgggctg gattggatta ctgggataag ctagagattt atttttacga gagtttggaa
     8401 gatttcatat ctcagatgaa gggaaaactc tatctgattt cgaaattcgc ggaaaaagtc
     8461 tattctgagg tggatttatc gactgacgag gaccattatt ttctctttgg acgtgaagac
     8521 aagggcttac ctgaagattt tatgcgagaa catcctgaga aggctctccg tattcccatg
     8581 aatgatgaac atgtccgcag tctcaatgtg tctaataccg tctgcatgat tgtctatgag
     8641 gctcttcgcc agcagaactt tgcaggtctt gagctcgtcc atacctatga agtggataaa
     8701 ttgaaataaa aaatgaaaat gaacaaaatg cttgcgcttg caagcgtttt ttgttatgat
     8761 aaaagagtct tcagggcagg gtgaaattcc cgaccggcgg taaatttgac tagctatttt
     8821 agctttctcg tcgttgtttt gtctcgatat accttaagta ttatcttcga ctgcgacgcc
     8881 tagataaatc taaaatatct tagcaaaata agtccgcgag cgcaagctga tgtggtgaga
     8941 ttccacaacc gacagtatag tctggatggg agaagacgaa agaatagctt tgtctgtttt
     9001 gatagattta tagccggact gtaaccactt gctttgtttt tctttaatag agtaagaggg
     9061 aacttttggg atataaaaag tgagaataga tagaggaatc ctttccaact tcttctgatt
     9121 ttatagaaaa ttggaggaac ctgttatgac aaacacacgt cgactttcga ccattgcaat
     9181 tctatcagcc atctcatttg tgctgatgta ctttgacttt ccgcttttac cagcggcatc
     9241 tttcctcaag atcgaattta gtatcttgcc agtccttgtg ggtctggtgg tcatggattt
     9301 gcctgctgct ctaggagtgc tcttgcttcg ctcactcttg aaattgcttc ttaacagcca
     9361 aggagtgaat acttacattg gtttgccgat gaatatcgta gctttgggag tttttgtcat
     9421 cgtatttgct ttgatttgga aaaaggaacg gacaaccctt cgtttcctac taggctctct
     9481 agctggaact attggtttaa ccttggccat gttggttctc aactatgttt atgctgttcc
     9541 tttgtacgct aagtttgcta actttgatat tggaaaaatt ttgggacttt ccaactacct
     9601 aatgaccatg gtattacctt ttaacttgat tgaaggtgtt atcttttccg tttcattctg
     9661 gttgttgtat gttctcttga aaccaacctt aaaacattat gagagataaa caaacatttt
     9721 taatgaaggg cagttttgcc cttttacttt tcgttattct tggctacatg gtcaaatttt
     9781 accctgaaac gctggtcaat tttgaccaat cgattcagac tgccattcga ggagacttac
     9841 cagattattt gactattctt tttcgagccc tcacacgtct gattgatatc ccagtgatta
     9901 tcacttgggt tgtcattaca gcttttgtct tttatcgtaa gcgatggaag atagaaagtt
     9961 tcttcatgct gggaaatctg gctttggcag gtcttttaat cgtgaccttt aaaaatatct
    10021 accagcgccc acgaccagct atcttacatc ttgtggagga gaagggattt tccttcccaa
    10081 gtgggcattc tctggctgta accttgatgg taggcactct gattgtcatt ctcagtcaac
    10141 gaattaaaaa tccagtctgg agaaaaatcg tacaaatcgt ccttggcctc tacctagtca
    10201 gtgtgctggt atcaagagtc tatctgggag ttcattaccc atcagacgtc cttgccagtc
    10261 tctgtgtggg cttgggagtc ctgtttatcg agtttccctt ctatgacaag ctccgcttcc
    10321 aatggcgatt taaaggcaaa cagaagtgag tatcgaattc ccttgaggag aaagaaatga
    10381 aagtcaatat agcagatctt catccgactc aactatattt atcagaaaag aagttgcaag
    10441 atattcagat gctttaccag tcggcagaaa caaaccaagt cgatccaatc agtattcttg
    10501 cctttggaga ttgtttgctg attacagatg ggcatcacag ggcttatcag gctttattgg
    10561 caggtcggga tacgatttct gctgagtggg atagagatgg tggtgatgaa ctatatcatc
    10621 tctatgcgca agcttgcgag gaaagaaaga tttactctgt ttttgattta gaagatcgta
    10681 tcctagctca agatgggtat gaagcaaaat ggtataactg gtgtgatggt tttaatcaag
    10741 cagcaactct tttgttgaaa aggtagtgca ttgtgttttg ttccttatta taaggtcaat
    10801 cattgtgagg aagcttttgc ttggtatcag gatgtgaact tggtttacct cgtagatggg
    10861 gtgaagcttc cttacagtca agcgaccttg gaagccatgt attcctattt ggatcggcat
    10921 ggtgagcttt tttggattga agtcaaggag aatggagaat ggtttccaat tggggatatt
    10981 acactatctc aggataatct ccccattgtg attgggaatt ccgcttacca acatcgagga
    11041 cttggaaaaa agattctaag tgctttgatt gaattggctc gagtaaaagg atggaaagaa
    11101 ttgagagtca agaaaatcta cacctacaat catgcttcta ggaggtgttt caagtcgctt
    11161 ggatttgtgg aaaatggagc aacagaaaaa ggaaggagtt ttatattgga attagtctaa
    11221 tcctgccact ttctccccct taacctttga ctattccgca aaattatcgt aaaataaaga
    11281 gtaaatgata aaatgaggtc agagtctgtt cgctctgacg atagtagtat aaatgaggag
    11341 aaacgctttg gaattagaag tatttgctgg gcaagaaaaa agtgaactat ctatgattga
    11401 ggtagcgcgt gctatcttgg aacttcgtgg tcgcgatcat gagatgcatt ttagcgatct
    11461 tgtaaacgaa attcaaaact accttggaac atcaaacagc gatatccgcg aagctttgcc
    11521 tttgttctac acagagttga actttgacgg tagcttcatc tcacttgggg acaacaaatg
    11581 gggtcttcgt tcatggtatg gtgtggacga aatcgacgaa gaaatcatcg ctcttgaaga
    11641 aaatgacgac gatgaagtag caccaaaagc taagaaaaaa cgtgtcaatg cctttatgga
    11701 tggtgattca gatgccattg actacaatgc agatgatcca gaagacgaag atgcatacga
    11761 agcagatcca gctctttcat acgatgatga aaatccagat gatgaaaaaa atgaagtgga
    11821 agcttatgat gcagaaatca acgaaatcgc tccagatgac ttgggagaag atgtggatct
    11881 caacgaagac gacgacgagt tttcagatga cgacgctgaa accagcgagg aataagagtt
    11941 agctattgac aattatccta tttttaggta tcatattgtt tgggcacctc ttttagaggt
    12001 cggggctccc tagttcttag ggagctattt ttgttttttc aagaagttat cttcttgtat
    12061 tttatactca atgaaaatca aagagcaagc taggaaacta gccgtaggct gttcaaaaca
    12121 ctgttttgag gttgtagata agactgacaa agtcaggaac acatatctac ggcaaggcga
    12181 cgttgacgcg gtttgaagag attttcgaag agtattagtt gtgaatctgg tgcagttgtc
    12241 ccagattatt cttattagta gggtcttgtt ttctatatcc cctcgtagtt aacaagacct
    12301 tgagcatttt agaaagagga atctatgtct acgaaatata tttttgtaac tggtggtgtg
    12361 gtatcgtcta ttgggaaagg gattgtggca gcgagtctag gccgtctctt gaaaaatcgt
    12421 ggtctcaaag taaccattca aaagtttgac ccttatatca atattgatcc gggaaccatg
    12481 agtccttacc agcacgggga agtttttgtg acagatgacg gagctgagac agatttggac
    12541 ttgggtcact atgaacgttt catcgatatc aatctcaaca aatattccaa cgtgacaact
    12601 gggaaaattt acagtgaagt tcttcgtaaa gaacgccgtg gagaatacct tggggcaact
    12661 gttcaagtca ttcctcatat cacagatgct ttgaaagaaa aaatcaagcg tgccgctcta
    12721 acgaccgact ctgatgtcat tatcacagag gttggtggaa cagtaggaga tatcgagtcc
    12781 ttgccattcc tagaggctct tcgtcagatg aaggcagatg tgggtgcgga taatgtcatg
    12841 tatatccata caaccttgct tccttacctc aaggctgctg gtgaaatgaa aaccaaacca
    12901 acccaacact ctgtcaaaga attgcgtggc ttgggaatcc aaccaaatat gttggttatt
    12961 cgtacagaag agccagctgg tcaaggaatt aaaaataaac tggcccagtt ctgtgatgtg
    13021 gcaccagaag ccgttatcga atcgttggat gttgaacacc tttaccaaat tccactgaac
    13081 ttgcaggcac aagggatgga ccaaattgtt tgtgatcatt tgaaattaga cgcaccagca
    13141 gcggctatga cagaatggtc agccatggtg gataaggtca tgaacctcaa gaaacaagtt
    13201 aagatttccc ttgttggtaa gtatgtggag ttgcaagatg cctatatctc agtggtcgaa
    13261 gccttgaaac actctggcta tgtcaatgat gcagaagtta aaatcaattg ggtcaatgcc
    13321 aatgatgtga cagcagagaa tgtagcagaa ctcttgtctg atgcagacgg gatcatcgta
    13381 ccaggtggtt ttggtcaacg tggtacagaa gggaaaatcc aagccatccg ctatgcgcgt
    13441 gaaaatgatg ttccaatgtt gggagtctgc ttgggaatgc agttgacatg tatcgagttt
    13501 gctcgtcacg ttttaggtct tgaaggtgcc aattctgcag agcttgcacc agaaacaaaa
    13561 taccctatca ttgatatcat gcgtgatcag attgatattg aggatatggg tggaaccctt
    13621 cgtttgggac tttatccgtc taagttgaaa cgtggctcta aggctgctgc tgcttatcac
    13681 aatcaagaag tggtgcaacg ccgtcaccgt caccgttatg agtttaataa tgccttccgt
    13741 gagcagtttg aggcagcagg ttttgtcttt tcaggagttt ctccagacaa tcgtttggta
    13801 gaaatcgtgg aaattcctga aaataaattc tttgtagctt gtcagtatca ccctgaactg
    13861 tcaagccgtc caaaccgacc agaagaactc tacactgcct ttgttactgc agcagttgag
    13921 aacagcaatt agcaaaatca gaacctttga gaaaaatctc agaggttttt tgcatacgat
    13981 gatattgcag tatatctgag gtaggggtcc tctgtatgta cctgctaccg ttgaaatcaa
    14041 tagcgactcc ctcttgccct gtgctagtga atggatttat cagtatattg aaatgaaata
    14101 aaatttgaac aaattaattc ggaaagccaa atcaatttct agcaaagttt taggaactgg
    14161 att
//
LOCUS       DAVKAO010000042        13571 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKAO010000042 DAVKAO010000000
VERSION     DAVKAO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13571
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.042"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            244..531
                     /locus_tag="VWX16_001384"
     CDS             244..531
                     /locus_tag="VWX16_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158723.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIFDGKAVEVTLREPYSRDILQVVKSIKQRQKLIAYRYKEGKLLFVKKEV"
     gene            533..676
                     /locus_tag="VWX16_001385"
     CDS             533..676
                     /locus_tag="VWX16_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158724.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKGLLKILEWSRCQALEQDSAYK
                     IGV"
     gene            689..985
                     /locus_tag="VWX16_001386"
     CDS             689..985
                     /locus_tag="VWX16_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158725.1"
                     /translation="MLQSPYAIDTINLKKDFLEKPIDIEKFKAFLEKEEIPLAIAWQG
                     DSLHFYTKDRSILDNHLDHLLEKMVNDPEKLSDFSMDKSLDDTIDEAKSQITFK"
     gene            complement(1219..2265)
                     /locus_tag="VWX16_001387"
                     /pseudo
     CDS             complement(1219..2265)
                     /locus_tag="VWX16_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2521..3329
                     /locus_tag="VWX16_001388"
                     /pseudo
     CDS             2521..3329
                     /locus_tag="VWX16_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            3397..3645
                     /locus_tag="VWX16_001389"
     CDS             3397..3645
                     /locus_tag="VWX16_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158726.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            3776..4948
                     /locus_tag="VWX16_001390"
     CDS             3776..4948
                     /locus_tag="VWX16_001390"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9158727.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKNLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            4948..6222
                     /locus_tag="VWX16_001391"
     CDS             4948..6222
                     /locus_tag="VWX16_001391"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9158728.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(6257..7222)
                     /locus_tag="VWX16_001392"
     CDS             complement(6257..7222)
                     /locus_tag="VWX16_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9158729.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(7203..8657)
                     /locus_tag="VWX16_001393"
     CDS             complement(7203..8657)
                     /locus_tag="VWX16_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9158730.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDCFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNSQVTVDRSQAGEQYAQEFGITRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            8912..9038
                     /locus_tag="VWX16_001394"
                     /pseudo
     CDS             8912..9038
                     /locus_tag="VWX16_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915902.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator T protein"
     gene            9033..10970
                     /locus_tag="VWX16_001395"
     CDS             9033..10970
                     /locus_tag="VWX16_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEU9158731.1"
                     /translation="MDYSKVAAEVIEAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLTHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGNAVIEVKI"
     gene            11119..12006
                     /locus_tag="VWX16_001396"
     CDS             11119..12006
                     /locus_tag="VWX16_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9158732.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(12326..12943)
                     /locus_tag="VWX16_001397"
     CDS             complement(12326..12943)
                     /locus_tag="VWX16_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9158733.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(13358..>13571)
                     /locus_tag="VWX16_001398"
     CDS             complement(13358..>13571)
                     /locus_tag="VWX16_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158734.1"
                     /translation="IMHTTIGSNKNHNRFIRKWLPKPKGTKKTTTKEVAFIEKRINNY
                     PKKCLNYKSPREDFWMTNLNLKFSNN"
BASE COUNT         4048 a         2586 c         2906 g         4031 t
ORIGIN      
        1 agctctacag aaaaataaaa aatgtgataa aaatttcaac tactcttatc tataataaag
       61 ctacctgttc tttgaaaatt gaatccactc cgtcttgatt agcgtgcctg tgtaagtagg
      121 gactgagagt atttccctgt gaagggcaac tggcacaaga cgtgtgtgag aattttctaa
      181 cagacacgga gtttatcgtt tccagactca aacgatgcga caaactagat aaaggagtta
      241 atcatgaagg tagtaaactt gtatgatttg aaacaaatgg gaaataaagg tggttgtacc
      301 atccaattga ttcatcactt tccttttggt atgggcttag gacatctcaa aaaggactac
      361 attgaattta aacgtgttgg tatctttgat gggaaagcag tagaagttac tcttcgagaa
      421 ccttattcga gagatatact gcaggttgtc aagtcgatta agcaacgtca gaaattgata
      481 gcttatcgtt ataaagaagg aaagctgcta tttgttaaga aggaggtctg agatgtcctc
      541 tgaacaacag gaacgaatgg cagttcaata tgctgagcgt agtcttttat ttactgtaaa
      601 aggtctttta aagatactag aatggtctag atgtcaggct ttagaacagg attccgccta
      661 taagataggg gtgtagaaat tagaagaatt gctacaatct ccctatgcga ttgatacgat
      721 taatctgaaa aaagattttt tagaaaaacc aattgatata gagaaattta aagctttttt
      781 agaaaaagaa gagattcctt tagccatcgc ttggcaaggg gattctctgc atttctacac
      841 gaaagaccgt tcgattctag acaatcattt agaccatctg ttagaaaaaa tggtgaatga
      901 tcctgagaaa ttatctgatt tttccatgga taagtcatta gacgatacaa ttgatgaggc
      961 taaatcccaa attaccttta aataatagag tatcgcaatt tgattgcgga actcttgttt
     1021 aatgagagtt gagataatat aaaggcgata ataattggga gaataattat attccccgcc
     1081 aaaccccact aagtttttat cttggtgggg tttcttggta cgacttaccc ttattaaaat
     1141 tattatgtag tttttttgaa tttagcatca tcaagattct gattgttttg atattgactt
     1201 ttcatttatt tctaatactc atctgttttc aaaaagcatt ctagtccatc tccgattaac
     1261 gatggacttt atcacctcct tctccagtcc ttgtatgaca tcttgaagtt gattcatgac
     1321 atcttccaaa gttcgaaagg ccttattctt aaatccacgt ttacgaatct ctttccacac
     1381 ttgttcaatg ggttcatctc tggtgtgtat ggaggaataa aggtaaaacc aatattagtc
     1441 ggaatcttta aggtacttga tttatgccat atagcattgt ccataacgag taaaagataa
     1501 tcatctggat aagcttgtga aagctcttct aaaaaggagt tcatccactc agtattacat
     1561 ccaccagcta ttaagaaaaa tgattcgcct gtatgggcat caacagctcc ataacaatag
     1621 tgaaattctc gtatatagtg actatggaca tgtggaccta ctcctattgg agaccaacaa
     1681 gatcccagtt tactgattct accgaaacca gcctcatctt ggtacatcaa gcgaacctta
     1741 cgaaaacgtc tactggtttt aaatcgcttt cttgtcttct tgaattgaga ttttattttt
     1801 agacgcgaca atggtttgag cgtctgcttt cttaggatgt tctggacgtg gcataatatt
     1861 tcgccagcca tggcgcttca acagttgata gaaggcatca cgtgtgtagg aacgacctaa
     1921 ctccttttta taagcctgaa ataaggcatc aattgtaaca aattctcctg cctctgtagc
     1981 cttcaaatgg cgggcaagaa agactttctc ttcctcaact gtcatatatg catggttacg
     2041 accaccacgt gtttcttgaa ggagagagtc gagtccgaac tcctcatatt tttttacatt
     2101 ttgctaaatc gttgtttaat tacagtctaa aagctctata atctctttat aagatttgcc
     2161 catcagacga aatagaacga tttgaaggcg tttatgatat ttagctgtac gagagtcttt
     2221 taaaagtgtt ttgatggttt ggatttcttc tttagttgat ttcatatcac tattgtataa
     2281 tgctttttga ttttagtcta gtataaatag cgagaaatat ctatcctatc ttctagaatg
     2341 tcttccaaac gaggaaactc tcgtaaacaa agaggtttta gaggcctatt taccgtggac
     2401 taaagttgta caagaaaagt gcaaataaga aatctccaga ttaggaacta tccgtgagtt
     2461 ctctagtctg gagatttttc aatagacttc ctgcgaaaca aaatatggta taatagttct
     2521 atgaatgatg aagcaagtaa acaactaacc gatgcacgat ttaagcgtct tgttggtgtt
     2581 caacgcacga cttttgaaga gatgttagct gtattaaaaa cagcttatca acttaaacac
     2641 gcaaaaggtg gacgaaaacc taaattaagc ctagaagact ttcttatggc cactcttcaa
     2701 tatgtgcgag aataccgcac ttatgaagaa attgcggctg attttggtat ccacgaaagc
     2761 aacttactcc gtcggagcca atgggttgaa gtaactcttg ttcaaaggtg gtgttacgat
     2821 ttcaagaact cctctcagtt ctgaagacac ggtaatgatt gatgcgacgg aagtaaaaat
     2881 caatcgccct aaaaaaaaaa caattagcga atgattctgg taaaaagaaa ttttacgcta
     2941 tgaaggctca agcgattgtc acaagtcaag gaagaattat ttctttggat atcactgtga
     3001 actattgtca tgatatgaag ttgttcaaaa tgagtcgcgg aaatatcgga caagctggta
     3061 aaatcttggc tgacagtaat tatcaagggc tcatgaagat atatcctcaa gcacaaactc
     3121 cacgtaaatc cagcaaactc aagccgctaa tagctgaaga taaagcctat aaccatgcac
     3181 tatctaagga tagaagcaag gttgagaaca tccttgccaa agtaaaaacg tttaaaatgt
     3241 tttcaacaac ctatcgaaat catcgtaaac gctttggatt acgaatgaat ttgagtgctg
     3301 gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctattatt ttccttattg
     3361 tctgtaagtc tactgacctt gttgtttatc ccagtcatgg tttctagttc gggctcagag
     3421 tttcaaagtg gatggcaaga gcatcaattg attgctgaga aggttagtaa aacacttgac
     3481 aagacatttg ataaggatgt cagagaaatt ccgaccagtc agttttatca aaaatttgta
     3541 gatgagatgg gaaggattta ctcaggaaat ttgatcctcc aggagctgat aactgtgaat
     3601 ggagcttata aagctactta tatcggtgaa ctctctagca actaatcatt ctttaaaatg
     3661 atgaccaata agaggcttga tttggtgaat caagtctttt ttctctcttt tactgccatt
     3721 tgtgataaaa tagtatagaa cgatttttta catgaatgat aaaacagagg taaatatgac
     3781 aatcggtatt gataagattg gttttgcgac cagtcaatat gtcttgaaat tacaagactt
     3841 agcagaagcg aggggaattg accctgaaaa attaagtaaa ggactcttac tcaaggaatt
     3901 gagtattgcg cccctaactg aggacatcgt gaccttggcg gccagtgcta gtgactctat
     3961 tttaactgag caagaaagac aagaagttga catggtcatt gtggctaccg agtcaggaat
     4021 tgaccagagt aaggctgcgg ccgtctttgt gcatggcttg ctgggcatcc agccctttgc
     4081 tcgtagtttc gagattaaag aagcctgcta cggagcgact gctgccctcc attatgccaa
     4141 attgcatgtg gaaaattctc cggagtccaa ggtcttggtc attgccagtg atattgccaa
     4201 atacggtatt gaaactccag gagaaccaac tcaaggtgct ggaagtgtag ctatgttgat
     4261 tacacaaaat ccacgcatga tggcctttaa taatgacaat gtagctcaga cccgtgacat
     4321 catggatttc tggcgaccaa attactcgac aactccttat gtaaatggtg tctattctac
     4381 ccaacaatac ttggatagtt tgaaaacgac ttggcttgaa tatcaaaaac gctaccagct
     4441 tactttggat gattttgcgg ctgtttgttt ccacttgcct tatcctaaat tagcgctaaa
     4501 aggcttgaaa aaaatcatgg ataagaactt gcctcaagag aaaaaagacc tcttgcaaaa
     4561 gcattttgac cagtctattc tctacagtca aaaggtgggg aatatctaca caggttcact
     4621 tttccttgga cttttgtctc tcttggaaaa tacagatagc ttgaaagctg gggataaaat
     4681 cgccctttat agttacggaa gtggagctgt ggctgagttc ttcagtggtg aattggttga
     4741 aggatatgaa gcttatttgg ataaagaccg tttgaacaag ctcaaccaac gaactgccct
     4801 atccgttgca gactatgaaa aggtcttttt tgaggaagta aacttggatg aaacaaactc
     4861 tgcccagttt gctggctatg aaaatcaaga ttttgccttg gttgaaattc tcgaccacca
     4921 acgccgttat agcaaggttg aaaaataatg aagataagtt ggaatggatt ttctaaaaaa
     4981 tcataccaag agcgcctcga gctgttaaaa gctcaggcgc tccttagtcc tgagagacaa
     5041 gctagtctgg agaaggatga acagatgagc gtgactgtgg cagaccagct gagtgagaat
     5101 gtagtgggaa ctttttctct gccttattca ctggttccag aggtacttgt caacggtcag
     5161 gaatacaccg ttccctatgt gacagaagaa ccctctgtgg ttgcggcggc cagctatgcc
     5221 agcaaaatca tcaagcgtgc aggtggtttt actgcacaag tccatcagcg acagatgatt
     5281 gggcaggtag ccctttatca agttgctaat cctaaactag cgcaagagaa gattgccagc
     5341 aagaaagcgg agctcttgga gcttgccaat caagcctatc cttctatcgt taaacgtgga
     5401 ggtggggcgc gtgatctgca tgtcgagcag ataaaaggcg aaccagactt tctcgttgtt
     5461 tatattcatg tcgataccca ggaagccatg ggtgccaata tgctcaacac catgctggaa
     5521 gccttgaaac cagtcttaga agaactcagt cagggacaga gtctcatggg aatcctgtcc
     5581 aactacgcga ccgattctct ggtgactgca agctgtcgca tcgcctttcg ctacttgagc
     5641 cgccaaaagg atcaaggacg agagattgcg gagaaaattg cgttggctag tcagtttgcg
     5701 caggctgatc cttaccgagc tgctactcat aataaaggaa tttttaatgg tattgatgcg
     5761 attttgattg ccactggtaa tgactggcgt gccatcgaag ctggggccca tgcctttgcc
     5821 agtcgagatg gacgctatca aggtcttagc tgctggacgc tggaccttga aagagaagaa
     5881 ttggtcggtg agatgaccct gcccatgcct gtagcgacta agggtggctc tatcggcctc
     5941 aacccacgtg tagctctcag tcatgatcta ctaggaaatc cttctgccag agaattagcc
     6001 cagattatcg tgtccatcgg tcttgctcaa aattttgcag ccctcaaagc cttggtaagt
     6061 acgggcatcc agcaaggcca catgaaacta caggccaaat ccctagctct cctagctggg
     6121 gctagtgaat ctgaagttgc tcccctagta gagcgcctca tctcagataa aacctttaac
     6181 ctagagacag cccagcgcta tctcgaaaat ttaagatcat aaaaactcag acgaatcggt
     6241 ctgagttttc ttgtgtttaa atactttttc ctggtaatag cgtaactggt aagaagtaac
     6301 cagttgtggc gacttccttg ccttcaatct tttgcaagag aagatcaata gtgagacaag
     6361 caatctcttc caaaggttgc ttgatagtag ccaattgagg gtagtaattt tcgataaagt
     6421 aggtcccatc atagccgatg accttgagct cttttgggac agaaatgccc aattcttgag
     6481 cgattttaat gaccagaata gctgtcaaat catccgaagc aaaaatggca tctggttttt
     6541 cccgggtcaa gatattcttg atttccattt cttttctgac gggagaaaag tcactggaaa
     6601 cattgataat aggagctttt gggagtacgg atgcaaaacc agcgtggcgc agtccggttg
     6661 gcgaattgga attgtcattc cctgtaatca tgatgataga ctgggcacct gtcttgacca
     6721 aggtttgggc agcaagaacc ccaccagcat agttgtcaga ggagacaaca gggatgtctg
     6781 gcgataggtt tcggtcaaag gaaataatcg gcgctgtcac acgattgtag tcttcgattc
     6841 ctaggttgtg actaccagaa atgatgccgt ctacctgatt ggcttccaac atttcgatgt
     6901 attcgcgttc cttctcagaa tcatgttcac tgttgcagat gatggtcttg taaccatttt
     6961 tgaagagttg gtgttccaat ttatcaatca attctgcata gaaaacattg gaaatattgg
     7021 ggaaaatcaa gccgattaac ttagctgatt ttccttgcag actacgagcc aggttgttgg
     7081 gtttatagcc caattctcgc atggcttcat tgactttttg gatggttttc tcagatagat
     7141 accctttttt attgataacc cgagaaacgg tagtaggact gacgcctgca agtttggcga
     7201 catcagttag ttttgcgacc ataatctaat tcatagtaag ttccagttgg gtttccagat
     7261 ttgatcagga taccattttg gtccgcatgt gggaagacac gaccagaaaa tactttttct
     7321 cctttattga tgaaaatttc aaagacagag ttatcgatga agattgtagc agtagtagcc
     7381 tgattctcga tagggcaaga acgagttatc ccaaattctt gggcatactg ttctccagcc
     7441 tggctacgat ccactgtcac ttgactgttt acaaggtcaa agttgattga aagtccctta
     7501 ccttctttat cagcaagtaa gacaatctcg ctctggctat tagcttccaa gttgagttca
     7561 agttcgtaag tgttcttggt ttgggaacgg tttgagaagg cttcttcaga agcacgaagg
     7621 tccttaatag cagcgactgg atactggtag agcttgtcgt ctttgatagt gagttccttg
     7681 accaaagaga aggttccttg gtgatcaaaa cagtcagatg ggtaagaaac atctggcaaa
     7741 ccaagccagc taactgctag agcacgccca tcaggagcgt tgaaggcttg agttgcatag
     7801 gcttcgaaac cgtaatccat gttttgaagt tgagacacat ctaccatttt ggcattttta
     7861 gggtcaaagg aagccccaat cttatacata tttggaaaga tattatcgta gtctagaact
     7921 ttcttatcca atccttgtgg acagtagaga aggacaggct gttcctctac aaagaccaga
     7981 ttaggacatt ccatcatgta ggcagtacgg tcgttagcaa agtcaaggtc gccaactgct
     8041 tgccagtttg tgtagtcgtt attgacagcc ttgtagagac ggacgaagcc ttttttctcc
     8101 aagtcctgtc caccgacgac agcatagtat tgacctttaa agttaaaaat ttgtggatcg
     8161 cggaagtggt cagtagagtc tgctggctgg tcaatcaaga tcttgtcaat ctttgtaatc
     8221 ttaccctcct tgtccatcaa agcaccgatc tggtatgggt gacggatcca gttttcatcg
     8281 cgaacatttc ctgtataaaa taggaataag ttatcgccaa attgcatggc agaaccagag
     8341 taggcaccgt ggctatctaa tggagtatct ggtaaaactt tgattccagt ttctttaaag
     8401 tgaaccaaat catcactttc tagctgtgcc caagatttta aaccgtgggc tgcaccaaaa
     8461 ggaaaattct ggtaaaagag gatccacttg ccatcaaagt aagaaaagcc atttgggtcg
     8521 ttgagaagtc ctgtttttgg ctcaacatgg taatgagtat gccatggaga ttgtgccata
     8581 ttttccttta tatgttgaat ttcttcttgc gtccaatctt gataaagtct gtaacgacgc
     8641 tcagttgtcc attccattca ttcctccaaa aatcttatca cttttaatag taactgtttt
     8701 cgataaaaaa tgcaagcctt ttatgttaaa aaagataaaa aactgtcaaa cgattatcat
     8761 aaaataaata caggaaatca attaaatttt tacgaaaatg tgaaagataa tgaaataaaa
     8821 gactttttaa ccaaggtttc aaacttattt ttcaaggaat tcctgatgaa acagtcaaaa
     8881 acaatcaatc taaagcttgc ccactaagta gatagaatat tttttctgca aaacgcttga
     8941 catattttta aaacatgata aaataatagt cgtaagggcg aataaaatcg ctttcaaaca
     9001 agaacaaaat gttatataag gagatttttg ctatggatta tagtaaagtt gctgccgagg
     9061 tcattgaggc tgtcggtaag gataatcttg tagctgcagc tcactgtgcg acacgtcttc
     9121 gcttggtatt gaaagacgaa gcaaaagtga atcaagcagc tctggataat aatgcggatg
     9181 tcaaggggac ttttagcact aacggacaat atcaaattat cattggtcct ggtgatgtta
     9241 acttcgtata tgcggaaatt attaagaaaa ctggtttgaa agaagtttcg acagatgatt
     9301 tgaaagagat tgctaataaa gacaaaaagt tcaatccatt gatggacttg attaaacttt
     9361 tgtcagacat ttttgttcct attatccctg ccttagttgc aggtggtctc ttgatggctt
     9421 tacggaactt cttgacatca ccagatttgt ttggacctca atcaattgaa gatatgtatc
     9481 ctgctatcaa aggcttctca gctatgattc aattgatgtc tgctgctcca tttatgttcc
     9541 tccctgtttt ggttggtatt tcagcagcca agcgctttgg agcaaaccaa ttccttggtg
     9601 cggcaatcgg tatgattatg accactccag atttgggtgg taaagaagcc ttctgggaca
     9661 tcttaggttt ccatgttaca caaacaaact atgcttatca ggttattcca gtccttgttg
     9721 cagtctggtt gttagctaat ttagaaaaat tcttccacaa gaaattgcca tctgcagttg
     9781 acttcacttt tacaccgctt ttatcagtga tgattacagg attccttacc tttacagtta
     9841 tcggtcctgt tatgttggtt gtgtctgatg cgattacaaa tgcgattgta tggttgtaca
     9901 acacaacagg tgcctttggt atggggctat ttggtggaac ttactcatta atcgttatga
     9961 cgggtcttca ccaatcattc ccagcaatcg aaacacaatt gttgtctgca tacaataata
    10021 acggtactgg atttggagac tacatctttg ttgttgcttc aatggcaaac gtagcacaag
    10081 gtgcggctac tcttgcagtc tacttcttga caaaaaatgc taaaacaaaa ggattgtcca
    10141 gttcggctgc tgtttctgcc ttccttggta ttacagaacc tgctctcttt ggggtaaact
    10201 tgaaatacaa attcccattc ttctgcgccc ttgctggttc agcaattggt gcctttgtag
    10261 caggtttaac tcacgttatt gcagtttcat tgggagcggc tggatttatt ggattccttt
    10321 ctattaaggc tggttcaatc cctatgtata taattgcgga aattatgtcc tttgttgccg
    10381 cctttgcttt cacttatttc tacgggaaaa caaaagcagc tagtgtcttt gctgatgaag
    10441 ctgccacagc aaccgcagaa acagttacag aacctacagt agaagcacca gtagtggaag
    10501 agactgatac tcttcaaaat gaaacacttg taactcctat cgttggtgat gttgtcgctc
    10561 ttgctgatgt caatgaccca gtcttctcaa gtggagctat gggacaaggt atcgctgtga
    10621 aaccaagcca aggcgtggtc tatgcaccag ctgatgctga agtttcaatt gcctttccaa
    10681 cagggcacgc ttttggtttg aaaacaagaa atggtgctga agttttgatt cacgttggta
    10741 ttgacactgt atctatgaac ggtgacggtt ttgaagcaaa agttgctcaa ggtaataagg
    10801 tgaaagctgg cgatgttctt ggaacatttg actcaaacaa aatcgctgca gctggacttg
    10861 atgatacaac aatggttatc gttacaaata cagctgacta cgcttcagta gctccagtcg
    10921 caacaggttc agttgcgaag gggaatgctg tgatcgaagt gaaaatctaa tcaatcctct
    10981 ctaatgtgaa aacgaacaaa tgacatgttt gttcgttttt attagatagt aagatttaca
    11041 gaggaaaatc taaaaaatag agacatttag actttcgaag tatgctataa taaagaaaat
    11101 aaaaacaaga ggtttatcat gacaaaatta tatggaagct tggaagcggg cggtacaaag
    11161 tttgtctgtg ctgtcggtga tgaaaacttt aacgttgtag aaaaaacaca atttccaaca
    11221 acaactccaa tcgaaacaat cgataaaacc attgagttct tctcaaaatt cgataacctt
    11281 gctggtcttg cagttggttc atttgggccg attgatattg acaaaaactc aaaaacttat
    11341 ggctttatca cgacgactcc aaaaccaaac tgggcaaatg tggacttgct tggtgccctt
    11401 cgtcgcgccc taaacgtgcc aatgtacttc acaacagacg taaacagctc tgcttacggt
    11461 gaaatggttg cccgtaacaa tgctggtggt cgtatcgaaa acttggttta ctacacaatc
    11521 ggtacaggta tcggtgcagg cgtcatccaa cgtggtgagt ttatcggtgg tgtgggtcac
    11581 cctgaaatgg gtcattatta tgttgctaga cacccaatgg atattgaaaa agaatttaag
    11641 ggtgtttgtc ctttccataa gggatgtcta gaaggttatg cagctggtcc aagtttggaa
    11701 gctcgtacag gtgtacgtgg ggaaactatt gaactcaaca accctgtttg ggatgttcaa
    11761 gcctactata tcgctcaagc tgcggttaat gcgacagtga ctttccgccc agacgtgatt
    11821 gtctttggtg gaggggtcat ggctcaacaa catatgctgg accgtgtccg tgagaaattt
    11881 acatctcttc ttaatggtta cctaccagta ccagatgtgc gtgactatat cgtgacgcca
    11941 gcagtcgcag gaaatggttc tgccacactt gggaactttg ttcttgcaaa agaagtttca
    12001 aaataaagca caaaatccgt ttggtttccc aagcggattt tgtggttgaa aacaatattt
    12061 ttgagccttt ccttaagtga ttacggacgg tagcgacttt cttcgaaatt ccatacctaa
    12121 actttgagcc tagtgtctca aagtttccga atacctgaaa ccattagatt tcaggtgttt
    12181 ctatcacggc ggaaaggcta ggtaagtcct cgcctcacta tcgaaaaata tgcatgattt
    12241 ccataccaaa gtatatttag tatcaagcta tagttaatct acgctttaaa aatccacttg
    12301 ggaaaccaaa cggatttttt acgtgtcaaa gcatttgcca gaaaaagtca ataatatagg
    12361 tcagaccgta ggtataaacc acgagggtaa agggtttggt cagaatgatg atggtcatgt
    12421 agcgcttgaa actcatcttg gtcagggcag ccagcataca gagaaagtca gctgggctaa
    12481 tgggccaaat catcataaaa ataaagaagc ggtcaaaacg attgccctta tctagccagc
    12541 cgatgtactt gtcgtaggtg cgcttgctga cgacagactg gacaaaggca gctccgtata
    12601 ggcgcactag ataaaagata atggcacagc caatcacgat gccgatatag ttgtagatag
    12661 tcccgatgat gtgcccgtag ataaagaccc cagccaccga ggtcaaggcc cctggaatga
    12721 tagggacgac agtctgtaaa atctgtaaaa agataaagag aggtggaccc cagatgcctg
    12781 cctgctggat aaaggcagag agggtttcct tggattgtaa aatcccagcc tgataagccc
    12841 aaatacagaa aatcaaactc ccaaccccac cgacaatcga tgagatattg ataactcgct
    12901 gcagaagggg agaaacagct ttcatttgtt tatcctgtga catgtaaact cctcatagat
    12961 agtatatttc tactatacca tattttggag gagaaagggg gaatgagtct tttttgataa
    13021 ggaatagaaa gtaaatagcc cttcgagact tagaataggt ctgtcaacta aaggttatat
    13081 gcattctagg tttcgaataa tcgcaattac ataaatttaa aagcattcta agtttcgaat
    13141 agcttatctg agataatttt gaggcattcg cgctttcgaa tagtcatttt ccctaaagtt
    13201 tagatgtatt ctgaagttag aaagactaaa atctaaaagt agtaagagtc tctctctgat
    13261 ggacgtaatt tatggctagt atgaatcctt tgaattgtta gttaatttta atattaatca
    13321 gttctgaaat aattactaaa tatagttaga tagtgaatta attattgcta aatttcaagt
    13381 tcaagttagt catccagaag tcttctctgg gggacttgta gttcaagcat tttttaggat
    13441 agttgttaat ccgcttttcg atgaatgcga cttccttggt agtcgttttc ttagttccct
    13501 taggcttagg taaccattta cgaatgaacc tgttgtgatt tttattagat cctatagtgg
    13561 tgtgcataat a
//
LOCUS       DAVKAO010000043        13467 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKAO010000043 DAVKAO010000000
VERSION     DAVKAO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.043"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..244
                     /locus_tag="VWX16_001399"
     CDS             <1..244
                     /locus_tag="VWX16_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158735.1"
                     /translation="KIYLFALRNKPELFAESDKRTVAKYKKLAESISEVNLNVFFQNA
                     LDFVEIFNQEPCLINFFEFMNSMLQGDIPKLTINQE"
     gene            260..442
                     /locus_tag="VWX16_001400"
     CDS             260..442
                     /locus_tag="VWX16_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEU9158736.1"
                     /translation="MKDTISNKDLISMGYRPSTANAIIHQVRELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            444..1571
                     /locus_tag="VWX16_001401"
     CDS             444..1571
                     /locus_tag="VWX16_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9158737.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKKYLMRKKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGETVSRNFLGRLNSSIQASIQMAIADKVIIEDFTAYVELFSSK
                     SGQKVEEKYLHTESDYQKVLVYLKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDE
                     VDELNEQLKTYRRYNTAIHKFTPPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELN
                     IDNENNLIFQHFGYVYDVPDIATVNKAIKVMLKELQIFPLITTKGARHTYGSYLWHNN
                     IDLGVIAKILGHKDISMLIDVYGHTLEEKISEEFTAVKSLL"
     gene            complement(1654..1726)
                     /locus_tag="VWX16_001402"
     tRNA            complement(1654..1726)
                     /locus_tag="VWX16_001402"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1691..1693),aa:Lys,seq:ctt)
     gene            complement(1991..3262)
                     /locus_tag="VWX16_001403"
     CDS             complement(1991..3262)
                     /locus_tag="VWX16_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9158738.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            3532..3771
                     /locus_tag="VWX16_001404"
     CDS             3532..3771
                     /locus_tag="VWX16_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158739.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            3761..4009
                     /locus_tag="VWX16_001405"
     CDS             3761..4009
                     /locus_tag="VWX16_001405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158740.1"
                     /translation="MKNRIIDVFEVVNRILVITVENPDFEDLRVNQFVKIGDKKYRVR
                     SGPMIHSTPPQSVLDRDTFTIDYTDDELLDKEAVFTTH"
     gene            4146..4325
                     /locus_tag="VWX16_001406"
     CDS             4146..4325
                     /locus_tag="VWX16_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9158741.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGK
                     LNKYTERGIRKQAGL"
     gene            4362..4814
                     /locus_tag="VWX16_001407"
     CDS             4362..4814
                     /locus_tag="VWX16_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9158742.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGLTVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            5105..5575
                     /locus_tag="VWX16_001408"
     CDS             5105..5575
                     /locus_tag="VWX16_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9158743.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPKY"
     gene            5594..6682
                     /locus_tag="VWX16_001409"
     CDS             5594..6682
                     /locus_tag="VWX16_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9158744.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            6663..7091
                     /locus_tag="VWX16_001410"
     CDS             6663..7091
                     /locus_tag="VWX16_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9158745.1"
                     /translation="MELEISDFPGCKIALFCEDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECAAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            7229..8179
                     /locus_tag="VWX16_001411"
     CDS             7229..8179
                     /locus_tag="VWX16_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9158746.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPETVDVAAVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            8181..8924
                     /locus_tag="VWX16_001412"
     CDS             8181..8924
                     /locus_tag="VWX16_001412"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9158747.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGSRILRAETAPLYALSALSVLVELEK"
     gene            8935..10731
                     /gene="pepF"
                     /locus_tag="VWX16_001413"
     CDS             8935..10731
                     /gene="pepF"
                     /locus_tag="VWX16_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9158748.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            10978..11769
                     /locus_tag="VWX16_001414"
     CDS             10978..11769
                     /locus_tag="VWX16_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9158749.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEGVSSDGGLDKEIDKIGLPTLERSFRALIYATLLSDDANQ
                     QSVFYQELNAGFRNDLSNQGLHYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPE
                     FPKNRVHEVFDILGQLFKRISIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEAREDFYKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            11880..12548
                     /locus_tag="VWX16_001415"
     CDS             11880..12548
                     /locus_tag="VWX16_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9158750.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(12573..12740)
                     /locus_tag="VWX16_001416"
     CDS             complement(12573..12740)
                     /locus_tag="VWX16_001416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158751.1"
                     /translation="MFEVVDKTDEVSSKHCFEVADRTDEVSNHTYGKVKLTWFEEIFE
                     EYHTKKPCSSR"
     gene            complement(12802..13335)
                     /gene="queF"
                     /locus_tag="VWX16_001417"
     CDS             complement(12802..13335)
                     /gene="queF"
                     /locus_tag="VWX16_001417"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9158752.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT         3962 a         2390 c         3124 g         3991 t
ORIGIN      
        1 gaagatttat cttttcgctc ttcgtaataa acctgaatta tttgcagaat ctgataaacg
       61 aacggtagct aagtataaaa aacttgccga aagtatctct gaagttaatt tgaatgtctt
      121 ttttcaaaat gctttagatt ttgttgaaat ctttaatcaa gagccttgtc tgataaattt
      181 ttttgagttt atgaattcta tgttacaagg cgatattcca aagttaacaa taaaccaaga
      241 ataataagag gggaacagta tgaaagatac gatttctaat aaggatttga tttccatggg
      301 ctatagacca tcaacagcaa atgctattat ccatcaggtg agagaattac ttgtatcacg
      361 aggctataca ttttataatc gcaaacgttt gatggttgtt ccaaaaagtg ttgtgaaaga
      421 gttgttggga atggaattgt gaaatggctt atatcgagta taaacagcgt gggaagaaaa
      481 ggctttggtc gttttctata cgtgagagga gcaagagcct actccataaa agcggattta
      541 aaacaaaacg agaagctaaa atagaagcgg agaaagttct tcataagtta aatactggga
      601 gtgtcttaag ctctagtatg actttatcgg agctttataa tgaatggctt gatttaaaaa
      661 ttttacctag caatagaagt gtagttacta aaaaaaaata tcttatgaga aagaaggtca
      721 tcgaaaggtt atttggaaat aagcctgtat cacaaattaa gcctagcgag tatcaaaaaa
      781 ttatgaatga gtatggagag actgtatcga gaaatttctt aggaagattg aattctagta
      841 ttcaggcaag tatacagatg gctattgctg ataaggtgat aatagaggat tttactgctt
      901 atgttgagtt gttctcttct aagagtggac aaaaggttga ggaaaagtat ttacatactg
      961 agtcagatta tcaaaaagtt ttagtatatt tgaaaaataa gtttgattat cagaaatcta
     1021 ttgtaccgta tgtaatttat ttccttttta aaactggcat gcgtttctcc gaattgattg
     1081 ctctaacttg ggatgaagtt gacgagctta atgagcaact taaaacttat cgtcgttaca
     1141 acaccgctat tcataagttt actccaccca aaaacaatac ttcaataaga ttagttccta
     1201 ttacttcaga tatgctttcc ttgttaaaaa cattgaaaat attgcaatta aaaacaaata
     1261 aagaattaaa tattgataat gaaaataatt tgatttttca gcattttggc tatgtctacg
     1321 atgttccaga tattgcaaca gtcaataaag ctataaaagt aatgttgaag gaattgcaaa
     1381 tttttccatt gattactacc aaaggagcta gacacactta tggtagttat ttatggcata
     1441 ataatattga tttgggagtt atagcgaaaa ttttaggaca taaagatatc tccatgttga
     1501 ttgacgttta tggtcacacc ttggaggaaa aaattagtga ggagtttacg gctgtgaaaa
     1561 gtttgttgta aaaacacggc aaattgcacg acaattttta aaaggaaagc aaaaaaagcc
     1621 ctatattata aggcttttcc tgttgtattt atatgccccc tacagggatc gaacctgtga
     1681 cccacggatt aagagtccgc tgctctgcca gctgagctaa ggaggcaaag aaaaaagctg
     1741 tattggtgcc gaaacttcac ggtttgtatt gaacccgcgc aattaagcag gtgggcaact
     1801 cgctctaact gaagctgttt ccgtgtgaga cggcctacat gctgttagaa gacttttgtt
     1861 tccctaataa tacaaaaaat agtcggtcaa cacttaagtg tgaagtcgta caccacagcg
     1921 tttctatatt tatatgatac cactttttca aaaaaaatca agaggaaagt gcaatttttt
     1981 gaaaaggatt tcagattttt cgcaaatggt cgatagcttc ctttctttga gccaaagccc
     2041 gttcatattt accagtatct tctgcttgga aatagtgatg attacgaatt ttttctggca
     2101 gatagtcttg cttgacccaa tttccaggat agttgtgtgg atagagatag tcttgggcat
     2161 tccccagttc cttgcttcca ctgtagtgcc catcacgtag gtgtcgcgga ataggcaagt
     2221 gccctgatgt tttgaggtca gcaagtgcct tatccatagc tacataggct gagttggatt
     2281 ttggagaaag ggccaaatca atcacgacat tggcaatgag aatgcgggct tctgggaacc
     2341 caatcttctg ggcagcatcc agagcagtca cggtatgaat ctgggcctca ggattggcta
     2401 agccgatatc ttcataggcg ataacagtca agcgacgagc gagactaggc agatccccag
     2461 cctcaatcaa gcgggcagta tagtggagac tggcatccac atctgagcca cggatagact
     2521 tttgcagggc agatagaaca tcatagtgac cgtctccatc cttgtccata gtgatgtagc
     2581 ttctctgaag actattttcc atgatgtcta aggtgatgtg gcgaatgccc tcgtcattct
     2641 caggggtaga gagaacagcc aagtccagtg agttaaaggc agagcgaagg tctccgtttg
     2701 tagaggttgc gatgaaatcc agcgcatcct catctagttc tattggaaaa tcaaaaccac
     2761 gttcagggtt acttagagct atctgcaggg cctctttgac gtcttggtta gacagaggtt
     2821 ccaactcgaa aatttgaact cggctacgaa tggcaggagt gacagagaag aaaggatttt
     2881 cagtcgttgc tccaatcatg atgaccagtc cactttccaa gagaggcaag aggaagtctt
     2941 gcttggtctt atctagtcga tgaatttcgt ctagcaatag gacgagacca ccagaaaatt
     3001 tagcttcttc cgagatttct tgcagtcgct ttttactatc aactgtcgca ttgaaagttc
     3061 gaaaggcata cttggtcgtt ccagcgatgg cagaggcaat actggttttg ccgattccag
     3121 gagggccata tagaatcatg gaggacaggc ggttggcttc caccatgcgg cggatgattt
     3181 ttccaggtcc gaccagatgc tcctgaccga tgacctggtc gatggtttta gggcgcatgc
     3241 gaagcgcgag attgtctggc atagcagtcc tttctaacgt ggatttgagt agaatgactc
     3301 atctactata tcttactatg tccattctag cctattagtt gtgatatttc aatggttttg
     3361 taataatcag ggagtgagat agaatctttt ttactattta atttaatgag gagaggtatc
     3421 agcatgagag atttgatttt ccttaaagct caaaatagga tagttgagcc taaagaagtg
     3481 gaagtgctga cttgagaaat aatattgctg aatgtcattg tatgaactat aatgaaaaga
     3541 gaggtgataa gcaatggaaa tgatggacca agtcaggaaa ttttgatttt tacaaaacaa
     3601 attcgtcatt ggattcttag tgatcaagtt atttcaggga aaagaaaact tttcttccgg
     3661 gaagatactc caaaagagat tttagatttg tacgaaaaca ttaaatctaa acttgatttt
     3721 gcttaccaag aagtttattc taataatggg ttgaaaaaat atgaaaaata gaataattga
     3781 tgtttttgaa gtagtaaatc gcatccttgt aataactgtt gaaaatcctg atttcgaaga
     3841 cttgagagtt aatcagtttg taaaaattgg agataaaaag tatagagtac gcagtggtcc
     3901 tatgattcat tcaactccgc ctcaatctgt cttagatcga gatactttta caattgacta
     3961 tacagatgat gaattgctcg ataaagaagc agtttttact actcattaga aaaataggta
     4021 tgaataaatt ggcttccctt agaaccacat ctaggggctt ttatgtataa tagaataata
     4081 agtatcactc ctttacaata cgtgtaacac gtgttataat ggtgttgtca ggaggtaaaa
     4141 gcgctatgcc tatgacacaa aaagagatgg ttaaactcct tacggcccat ggttggataa
     4201 agactagagg cggtaaaggc tcccatatta aaatggaaaa gcaaggggaa agacctatca
     4261 caatccttca tggtaagctg aataagtaca ctgaaagagg gataagaaag caagctgggt
     4321 tgtaaaattc agcccctgct ttttgatata gaggtagtga aatgttagtt acgtatccag
     4381 ctttatttta ttatgacgac acagacggaa cagaagcgac ttattttgtc catttcccag
     4441 attttgaata ctcagctaca caaggagagg ggatttctga ggctttggct atggggtcgg
     4501 agtggctagg gctaactgtt gcagatttga tagagagtga tggagagtta cctcagccat
     4561 cagatatcaa tagcttgtct ttaattgata atgatccttt taaagatgat gaagatttcg
     4621 tgtcaaccta tgaccttgat aaatctttta tttctatggt atctgttgat gtatcagaat
     4681 acctaggaag tcaggaaccc attaaaaaga ctttgaccat accaaaatgg gcagataagt
     4741 tgggacgaga aatgggactt aacttttctc agactttgac agacgctatt gcagataaga
     4801 aagttcaagc ctaagattaa gcgtggtatc gtggtaatag tcaataaaaa agcacgtcca
     4861 cttgtgctag ttactcgcct attgatttta tagatttatt agccctttcg agggcttttt
     4921 atattgattt ttatgagaaa tgaagaaatc agacttttaa gaaaatcatt gataccaagg
     4981 gtttaaatga ggtaatatgg tataattagg acataaaata attttgtggt aagatggtag
     5041 tatctatttt agcatatttc cgagcaatcg gggcgattaa agagtcgcat agaaagagga
     5101 caaaatggca acatacggat ttttagatat tttagaggaa gagttggaca agaactttcc
     5161 ctttgacttt gagattagct gggataaacg caaccatgcg gtagaagtga gttttctatt
     5221 ggaagcgcaa aatgctgcag gtgtggagat ggtggatgaa gacggagagg tttcatcaga
     5281 tgacattctt tttgaggaag cggtcctttt ctacaatcct gccaagtcaa cagtcaatga
     5341 ggaagactat ttgactgtta ttccttacct gcctaaaaaa ggattttccc gtgaattttt
     5401 agcttatttt gcgcttttcc ttaaagatac tgctgaggtt gggctagatg tcctcatgga
     5461 ctttttggaa gacccagaag cagaggaatt tgtcatggaa tggaaccaag aagtctttga
     5521 agaaggaaaa gttggcttgg aagagggaga attttatcct tatccaaaat actaggagtt
     5581 ggttggaggt gttatgaaga aaattgggac ttatttggtt tatgtgctag ctgttgtctt
     5641 tattatgcta gcttttgctt gtggaacaat cgcatttgca gagttggggt attccgcagt
     5701 tttagccttt acttttggtt atgccttcgc tcttctaagc atgtatttaa tctttattct
     5761 tcatgagctg ggtcatgctt tttgtggcta cttgacaggc tatcggctgg tggcttttgg
     5821 attaggacat tttattttga ccaaaaagtc aggcaggttt catcttagta gaacagccat
     5881 tctgaaaaat gttggtgctc aatacattgg tttaaaagag gatgaaagcg atcaaagaat
     5941 catcctgatg ctttcaggag gcttgatggt tcatctcggc ttgatattat tggcgatatt
     6001 gtttggattt ttgacaagaa gctggtattt tgcagggact tggatttttc ttaatttatc
     6061 tttcttccta aataacattt tgccagtcga catcaccgat ggagcaaaaa tttgggaatt
     6121 actacaacac cctgaaaata ccaaatacgc ctacctggta ttgagacatt ctgctcaaac
     6181 tttgctggct cctcaagaat atgatttgaa agactttgtt caagcagttc ctgaggatgc
     6241 acgagggagt tttgcggatg gtgtattagg tatgcaaggt gaagtttcca ttttggaagg
     6301 gaaggaagaa gtagctaaac agcagtttca agcattactt gagaggacaa ctactccgat
     6361 gatgcagaca gttgcccaac tatccctgct tcatatcgcc ttgttggaag gagattttga
     6421 gcaagcggag caatatgcta gtattcgaca ggtcaaatcg tttctgtctc tcaaactgtc
     6481 caatcttcaa gtgatacaag cctggtatca attaaaggtg aagaaagatg tagatcgaac
     6541 tcgtaaggct ctcaagattg ctagacagaa aatggatagt agtcgaatgt tgagggacga
     6601 gaaacgctat tatgaaaact tgttagcaga gctggaaaag gaaatgttag aaggaactga
     6661 agatggaact agaaatttct gatttcccag gctgtaagat tgctttgttt tgtgaggata
     6721 agctcttgac tatcttacgt gatgataagg caagcattcc ctgggccaat atgtgggaac
     6781 tgccaggtgg gggacgtgaa ggtgatgaaa gtccttttga gtgcgcggcg cgtgaagttt
     6841 atgaagaact gggcattcat ctgactgaag attgtctgct ttggagtaag gtttatccaa
     6901 gtatgctttt tgcggataaa caatctgtat ttctagttgg tcagttgaca cagaaccagt
     6961 ttgacagtat cgtatttgga gatgaaggac agggctatca gctgatgaat gttgaggaat
     7021 ttcttagttc cagtcaagtt gtccctcagt tgcaagagag attaaaagat tatttaaaag
     7081 taagtgatta gaaaggatgg ttcggttaaa tttctaaact gaacccgccc taaacacggt
     7141 gccaaaaaga taaacttctc ttagacacaa acgtcttcag agagttacct attttggctt
     7201 tgtgttttac gggcttggta tcttaataat ggaaacatgg caagagttaa aagttacagt
     7261 gaagcgtgag ggagaggagt tggtttccaa tctcttgatt gagttaggtg cccaaggggt
     7321 agcaattgaa gacagcatgg actatgtggg aaatgtggac cgattcggtg aaattttccc
     7381 agaggtggag caacaggaag aaatcgtagt gaccgcctac taccctgaaa cggttgatgt
     7441 agcagcagtt gaagcggatt tgcaggctcg tctggcagaa ttgaccgact ttatggacct
     7501 aggagaagtc aagatgggca cgactgcctt ggctgaggaa gactgggctg acaactggaa
     7561 gaagtattat gaaccggcac gcatcaccca tgacctgacc atcgtaccgt catggacgga
     7621 ctatgaggca acagcaggag aaaaaattat caagctggat cctggcatgg cttttggtac
     7681 tggaacccac ccaactacca agatgagcct ttttgccttg gaacaggttc ttcgtggtgg
     7741 cgaaacggtg ctagatgtgg ggactggttc aggggttctc tctattgcca gctcgcttct
     7801 gggggctaaa gaaattttcg cctatgacct ggatgatgtg gcggttcgtg tggctcagga
     7861 aaatattgag ctcaaccctg gcatggaaaa tatccatgta gcggctggag atttgcttaa
     7921 gggtgtggaa attgaggcag atgtgattgt ggctaatatc ttggcggata tcctcattca
     7981 tctaatagac gatgcctatc gtttggttaa ggacgaaggc tacctgatca tgagtggcat
     8041 tatcaaggat aagtgggaca tggtgcgcga gtcggctgaa tcagctggat ttttcctcga
     8101 aactcacatg gttcaagggg aatggaatac ctgtgtcttt aagaaaacca aggatatctc
     8161 tggtgtgatt ggaggctagc atgcagcagt attttgtaaa aggcagtgct atctctcctg
     8221 tcactatcga ggacaaggaa accagtaagc atatgtttca ggttatgcgc ttgaaagaag
     8281 aggatgaggt taccttagtc tttgatgatg gcatcaagcg cttggcgcgc gtgctggata
     8341 tggaaaatcg tcagtttgag ttggtccaag aattaaatga caatgtggaa ctaccagtcc
     8401 aagtgaccat cgcatctgga tttcccaagg gagacaagct ggagttcatc gctcaaaaag
     8461 taaccgaact gggtgctagc caaatctggg cctttcctgc agactggtca gttgccaagt
     8521 gggatggcaa gaaattgggt aaaaaagttg aaaaactaga aaaaattgcc cttggagcag
     8581 ccgagcaaag caagcgtaat attgtaccaa gtattaaact tttcgagaaa aaagcagatt
     8641 ttctagctca gctggaccag tttgattcta tcatagtagc ctatgaagaa tcagctaaag
     8701 aaggagaagc cgctgcgctt ctgcaagcag tcactggtct tgaaacagga gccaaactgc
     8761 tctttatctt tggtccagaa ggtggtctgt cacctgcaga aatcgaaagt tttgaagcta
     8821 aaggagcagt tttggcaggc ctaggttctc gtattttgcg agcagaaaca gcaccgcttt
     8881 acgccttatc agcccttagt gttttagtag aattagagaa ataagaggaa gaaaatggaa
     8941 caaaaacacc gttcagaatt tccagagaag gaactctggg acttaacagc cctataccaa
     9001 gaccgtgagg atttcttgcg tgcaatcgag aaagctcgtg aagacatcaa ccagtttagc
     9061 cgtgattaca agggcaatct tcacactttt gaggatttcg agaaggcctt tgcggaattg
     9121 gaacagatct acattcagat gagccatatt ggcaactatg cttttatgcc tcagacgacg
     9181 gactatagca atgacgaatt tgccaatatt gcccaagctg ggatggaatt tgaaacagat
     9241 gccagcgtag ccttgacctt ctttgacgat gccttggtgg tagcagacga ggaagtcttg
     9301 gaccgtttgg gtgaattgcc tcacttgacg gcagctattc gtcaggccaa aatcaaaaaa
     9361 gcccactatc taggggctga tgtggagaag gccttgacca atctcggtga agttttctat
     9421 agtccgcagg acatttatac taagatgcga gctggggatt ttgaaatggc tgactttgaa
     9481 gcccatggca agacctataa aaacagcttt gtcacctatg agaatttcta ccaaaatcac
     9541 gaggatgctg aggttcgtga gaaatccttc cgttccttct cagagggact tcgtaagcac
     9601 caaaatacgg ctgcagcagc ctatctggct caggtcaagt ctgaaaaact cttggctgat
     9661 atgaagggat acgactctgt ctttgattat cttctagctg aacaagaagt ggaccgtgtc
     9721 atgtttgacc gccagattga cctcatcatg aaggactttg caccagtcgc tcagagatac
     9781 ctcaagcatg ttgccaaggt aaatggtctt gaaaagatga cctttgcaga ctggaaattg
     9841 gacttggaca gcgccctgaa tcctgaagtg actattgacg atgcctatga tttggtcatg
     9901 aagtcggtag aacctttggg gcaagaatat tgtcaggaag ttgctcgtta ccaagaagag
     9961 cgctgggtgg actttgctgc taacagtggc aaggattccg gtggttatgc ggcggaccca
    10021 tatcgcgtac acccttatgt actcatgagc tggacaggcc gtttgagcga tgtctatacc
    10081 ttgattcatg aaatcgggca ttctggtcaa ttcatctttt cagacaatca ccaaagctat
    10141 ttcaacgccc acatgtcgac ctactatgtt gaagcaccgt caaccttcaa tgaattgcta
    10201 ctcagtgatt acttagagaa ccaatctaat gacccacgtc aaaaacgctt cgctctggct
    10261 catcgcttga cagacaccta cttccataac tttatcaccc acctcttgga agcagccttc
    10321 cagcgtaagg tgtatacatt gattgaagaa ggagaaacct tcggagcaag caaactcaac
    10381 agcattatga aggaagtttt gacagatttt tggggcgatg ccattgaaat cgacgacgat
    10441 gcagctttga cttggatgcg ccaagctcac tactatatgg gcttgtatag ttacacttac
    10501 tctgctggtc ttgtcatctc gacagctggt taccttcatc ttaaaaactc agaaactgga
    10561 gctgaagact ggctcaatct cctcaaatca ggtggtagca agacaccact tgagtcagcc
    10621 atgattatcg gtgctgatat ttcaacagac aaaccactcc gtgataccat ccaattcttg
    10681 tctgacacag ttgaccagat tatcgcctac agtgctgagt tgggggagta aggggaatag
    10741 aaaaattttc tagaatgatg tttatgagct tgttaagcgt cattctattt gtcttagttg
    10801 tagctatttt caaaaatgtc gttgaaggtc gtgatgttaa atttgaactt actgtagaac
    10861 tggtattaat agttatttac gtcattttat atcagattgt atttaattga gaattcaata
    10921 ttgagtcatt taagactgcc tttttgaaca gttgctagaa atgtagaggt attgaccatg
    10981 tatcaagatt tactaagaaa aatagcagaa gaaaaaccaa attataacca ggaggaaatc
    11041 cagtggttgc ttgatcattt gggagatcct tctccagaaa ttcgtgatga ccttgttttt
    11101 acaagctttg ctagagggat tcaggaagag ctatttacac aggagcaatt tcatttcatt
    11161 gctgaagggg tttcatctga tggagggtta gataaagaga ttgataagat aggcctgcca
    11221 acacttgaac gttcttttag ggcgcttatt tatgcaactc tcttgtctga cgatgccaac
    11281 cagcaatcgg ttttttatca ggaattaaat gcaggatttc gtaatgacct ttcaaatcaa
    11341 ggcttgcact atctttcaaa agaaaaggat acaacaggtt tctcaagtca gtatggttgg
    11401 gttcattctt ttgcacatgg agccgattta ctgacagagg tggtttgtca tccagagttt
    11461 cctaaaaaca gagttcatga agtatttgat atacttggcc aactatttaa aagaatttcg
    11521 attcgcttta cagatgatga ggattggcgt ttagcaagag tgatctatga acctatttta
    11581 caagggaagt tggagcaaga acaagtagct tcttggataa aaactgttga ttttccgata
    11641 gaagcaaggg aggattttta caaattttcc aactttagat cctgtctggt ggaagtctat
    11701 gtccaacttg accagagaaa tagtttacaa gatgagttga aagaagctat ccagtctttt
    11761 caatactaga gattagctaa tagttctatg attttcaaaa aatttccaat caattttctt
    11821 gaaacccttt cctacttttg atagactagt atctagtttt tgaaaaaagg agaaagaaaa
    11881 tgaaaaaatt tgttgctgag ttaatcggta cgttcatgct tgtgttcgtt gggacaggag
    11941 ctgttgtttt tggaaatggt tttgatggcc ttggtcacct tggaatcgcc tttgcctttg
    12001 gtttggcaat cgtggtggca gcctactcaa tcggaactgt ttcaggtgct cacttgaacc
    12061 cggctgtttc gattgctatg tttgtaaaca aacgtttgtc atcttcagaa cttgtaaact
    12121 acatccttgg tcaggttgtt ggagctttca tcgcttctgg cgcagtcttc ttcctcttgg
    12181 gtaattcagg tatgtcaact gctagccttg gtgaaaatgc cttggcaaac ggtgtcactg
    12241 tctttggtgg tttcttgttt gaagtcatcg caactttctt gtttgtcttg gttatcatga
    12301 ctgtgacttc agaaagtaag ggcaatggcg cgattgctgg tttggtaatc ggtttgtcat
    12361 tgatggcgat gattcttgtc ggattgaaga ttactggact ttcagtaaac ccagctcgta
    12421 gcttggcacc agctgtcttg gtaggcggcg cagcccttca acaagtttgg attttcatcc
    12481 ttgcaccaat cgctggtgga gttcttgcag cccttgttgc aaaaaatttc cttggaacag
    12541 aagaataatt gaaactcaaa aagccttgct cctcatcttg aggaacaggg ctttttcgta
    12601 tgatactctt cgaaaatctc ttcaaaccac gtcagcttca ccttgccgta ggtatggtta
    12661 ctgacttcgt cagttctatc tgcaacctca aaacagtgtt ttgagctgac ttcgtcagtc
    12721 ttatctacaa cctcaaacac tgctttgagt aacctgcggc taacttccta gtttgctctt
    12781 tgattttcat tgagtatgac tttagcggtt gtcaattttc tctggataaa ggtcgtgttg
    12841 gaagaggcgt tgttctgcca agccctcata cttagttcct tgcttaccgt agttatagta
    12901 ggggtcgatt gaaatgccac cgcgcggagt gaattttccc cagacttcta aatagcgagg
    12961 gtctagcaag ttgaccaagt ctttcccgat ggtgttgata cagttttcgt ggaaatctcc
    13021 gtggtttcgg tagctaaata gatatagttt gagggatttt gactcgacac agagcttgtc
    13081 aggaatgtag gaaatatgaa tcgtcgcaaa gtctggctga gcagtgattt gtcccagcag
    13141 agacatatcg aggatatggt gacgaatgcc ctgttcctta gcgatttctc tagtaatttg
    13201 aatttcgagg tgatgacgtt ggccgtaggc aaaggtgaca gcttcgactg tttcatagtg
    13261 ttgcatggcc cagaaaaggc aggttgttga atcttgacca ccactaaaga cgaccaaggc
    13321 tgattgacgt ttcatagtac tccttccaaa atgggaaatg ttcagagcac gcaaaaagct
    13381 cccattaggg agctaaaaaa taccaaatcg aggttttttt agcgatggca tatcccaaac
    13441 atcgtaatat tctacttata tagtaaa
//
LOCUS       DAVKAO010000044        13143 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKAO010000044 DAVKAO010000000
VERSION     DAVKAO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13143
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.044"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            60..1319
                     /locus_tag="VWX16_001418"
                     /pseudo
     CDS             60..1319
                     /locus_tag="VWX16_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            1415..2689
                     /locus_tag="VWX16_001419"
     CDS             1415..2689
                     /locus_tag="VWX16_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9158753.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIENFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKEHDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKVQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            2699..3577
                     /gene="truB"
                     /locus_tag="VWX16_001420"
     CDS             2699..3577
                     /gene="truB"
                     /locus_tag="VWX16_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9158754.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAADLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            3818..4120
                     /locus_tag="VWX16_001421"
     CDS             3818..4120
                     /locus_tag="VWX16_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9158755.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKHQS
                     VMADLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            4228..4866
                     /gene="udk"
                     /locus_tag="VWX16_001422"
     CDS             4228..4866
                     /gene="udk"
                     /locus_tag="VWX16_001422"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9158756.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            4994..6334
                     /locus_tag="VWX16_001423"
     CDS             4994..6334
                     /locus_tag="VWX16_001423"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9158757.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSRIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            6312..6524
                     /locus_tag="VWX16_001424"
     CDS             6312..6524
                     /locus_tag="VWX16_001424"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9158758.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            6521..7396
                     /locus_tag="VWX16_001425"
     CDS             6521..7396
                     /locus_tag="VWX16_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9158759.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVGLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            7389..8204
                     /locus_tag="VWX16_001426"
     CDS             7389..8204
                     /locus_tag="VWX16_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9158760.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            8197..8628
                     /locus_tag="VWX16_001427"
     CDS             8197..8628
                     /locus_tag="VWX16_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9158761.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            8635..10302
                     /gene="recN"
                     /locus_tag="VWX16_001428"
     CDS             8635..10302
                     /gene="recN"
                     /locus_tag="VWX16_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9158762.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            10304..11032
                     /locus_tag="VWX16_001429"
     CDS             10304..11032
                     /locus_tag="VWX16_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9158763.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            11111..12934
                     /gene="lepA"
                     /locus_tag="VWX16_001430"
     CDS             11111..12934
                     /gene="lepA"
                     /locus_tag="VWX16_001430"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9158764.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         3891 a         2421 c         3167 g         3664 t
ORIGIN      
        1 tgtaaactat aactcaaggt gctacaatat ccttaataaa ataatatgga ggtcacctta
       61 tgactcgtga ttttaaattt gaaactctac aactacatgc tgttcaagtt gtggctccag
      121 ctactaagtc tcgtgcagtg ccgatttatc aaacaacatt ttttgttttt gatgacacgt
      181 aggaaggtgc cgatatgttt gccttgagga aaccagggaa catttatact cgtatcacca
      241 atcctacaac agctgccctt gaaggtggtg ttgaagcgct agcaacagca tcaggtatga
      301 ctgcagtgac ttatacgatt ttggcgattg cccatgctgg tgaccatgta gtggctgctt
      361 cgactattta cggtggaacc ttcaatcttt tgaaagaacc ccttcctcgt tatggtatca
      421 caacaacctt tgtcgatatt gataatttgg aggaagtaga agcagctatc aaagacaata
      481 ccaagcttgt cttgattgaa accttgggta accccttgat taatattcca gacctggaaa
      541 aactggcaga gattgctcat aaacatcaaa tcccacttgt gtcagacaat acttttgcaa
      601 caccttattt gattaacgtc ttctctcatg gcgttgacat tgccattcac tctgcgacta
      661 agtttatcgg tgggcatggt acaactattg gaggaataat tgtcgatagt ggtagttttg
      721 actggacggc ttcagggaaa ttccctcaat ttgttgacga gggtccaagc tgccacaatt
      781 tgagttatac tcgtgatgtg ggtgcagcag cctttattat agctgttcga gttcaattgc
      841 ttcgtgatac aggtgcagcc ttgtcaccat tcaatgcttt cctcttgcta caaagacttg
      901 aaaccctttc acttcgtgtg gaacgccatg tacaaaatgc tgagacaatt gttgattttc
      961 ttgtcaacca tcctaaggta gaaaaggtaa attatccaaa acttgcagat agtccttatc
     1021 atgccttggc tgagaaatac ttaccaaaag gtgtgggctc aatctttacc ttccatgtca
     1081 aaggtgggga ggccgaagcc cgcaaggtca ttgataattt ggaaatcttc tcagaccttg
     1141 ctaacgtggc agatgccaag tctcttgttg ttcatccagc aacaaccact cacggtcaat
     1201 tgtcagacaa agacctagaa gccgcaggtg ttacaccaaa ccaaatccgc ttgtcgatcg
     1261 gacttgaaaa tgtagaggat ttgattgaag atttgcgctt ggccttggaa aaaatttaaa
     1321 gtaaaagaag ataaacagtg gttttcgact cgctgttttt gattttcctt catgcatgat
     1381 ataatggtta cagaagtcta gaaagaggaa tgtaatgaac gaaatcaaat gtcccaactg
     1441 tggggaagtc tttacagtaa atgaaagcca gtatgctgaa cttttgtccc aagtgagaac
     1501 ggcagagttt gataaggaac tacacgatag gatgaggcag gaactggcct tggctgagca
     1561 aaaggctatg aatgagcaac agactaaact ggctcagaag gatcaagaaa ttgcgcaatt
     1621 acagagtcag atcgaaaact ttgatacaga aaaagaattg gccaagaaag aagttgaaca
     1681 gacaagccat caggccttat tggctaagga caaggaagta caggctttgg agaaccaatt
     1741 ggccactttg cgtttggagc atgaaaatca actgcaaaag accctttctg acctagaaaa
     1801 agaacatgat caggtcaaaa atcagctcct cttgcaagaa aaggaaaatg agttgtcttt
     1861 ggcttctgtt aagcaaaact atgaagccca gcttaaagca gctagtgaac aagtcgagtt
     1921 ttataagaat tttaaggttc aacaatctac aaaagcgatt ggggaaagcc tagaacagta
     1981 tgcagagagt gagtttaaca aggttcgtag tttcgccttt ccaaatgctt actttgagaa
     2041 ggataacaag gtctcttcgc gtgggtctaa aggggacttt atcttccgtg agtgtgatga
     2101 aaatggagtt gaaatcattt ctatcatgtt tgagatgaaa aacgaagcgg acggaacaga
     2161 gaagaagcac aagaatgcag atttttacaa ggaattggac aaggaccgtc gggagaagaa
     2221 ctgtgagtat gccgttttgg tgaccatgct tgaggctgat aatgactact ttaacacagg
     2281 gattgttgac gtcagtcacg agtatgaaaa aatgtatgtt gttcgtcctc aattctttat
     2341 ccagttgatt ggtctcttac gtaatgcggc gctaaattcc ctaaaataca agcaggagtt
     2401 ggccttggtt cgcgagcaaa atattgacat tacgcatttt gaggaagact tggatgcctt
     2461 taagctagct tttgctaaga actataattc agcttcgact aactttggaa aagctattga
     2521 tgaaatcgac aaggccatca aacgcatgga agaggttaag aaattcctga ccacatctga
     2581 aaaccaactc cgtttagcta acaacaaatt ggaagatgtc tctgttaaaa aattgacccg
     2641 gaaaaatcca acaatgaaag cgaagttcga agcactgaag ggggagtaga aagcaaaaat
     2701 gaacggtatt atcaacttaa aaaaagaagc aggaatgacc tcgcatgatg cggtttttaa
     2761 actgcgtaag attttgggaa ccaagaaaat tggtcatggt ggaaccttgg atccggatgt
     2821 ggtgggtgtt ttgccgattg cggttggcaa ggcgacacgc atggtcgagt ttatgcagga
     2881 cgagggtaag atctatgagg gggaaattac tctgggctat tccacgacga ctgaggatgc
     2941 tagtggggaa gtggtcgcag aaacccctgt tttgtctccc ttggatgaaa agcttgttga
     3001 tgaagcgatt gctagcttga ctgggcctat tacccagatt ccccctatgt attcggcagt
     3061 taaggttaat ggtcgcaagc tctatgagta tgcgcgtgct ggtcaggaag tggagcgtcc
     3121 agaacgtcag gtgaccattt atcaatttga gcgaacaagt ccgatttctt atgatggcca
     3181 acttgcccga ttcacttttc gtgtaaaatg cagtaaaggg acttacatcc gtactttgtc
     3241 agttgatttg ggtgaaaagc ttggttatgc ggctcatatg tcccatttga ctcgtactag
     3301 tgctgctgac ttacaattag aagacgctct tgccttggag gaaattgctg aaaaagtaga
     3361 ggctgggcaa ttagattttc tccatccttt agagattggg acaggtgacc ttgtcaaagt
     3421 tttcctaact ccagaagagg ctacagaagt tcgctttggt cgttttattg agctagacca
     3481 aacggataaa gaactggctg cctttgaaga tgataaattg ttagccattc tagaaaaacg
     3541 gggcaatctc tataagccaa ggaaggtttt tagctagatc gtttaggaat aaaaatcggg
     3601 tgatagataa caattgcttg ataaaacccc atactaatag tagaatggtt ttgggaatta
     3661 taatattcca attgttgcga gttgtaggta ctcaaataat ctatatagaa atttagaggt
     3721 gtgaaatgaa gcaatttaaa attctttcag ataaatattt agagtccatt acaggttctg
     3781 gtgggaactt aggcccagga tttggtgtga taattccatg atgcgaaatg agtttcgaga
     3841 aagggtggag caacttcttc aacaaaaaga aataaatgaa aatagtgagt tgagtcacct
     3901 gtttcgtctt gctatacaaa atttagacag aaatgaaaaa caccaatcgg tcatggccga
     3961 tttgagtcaa gggttgtcac tttacctcat gacgcatcat taccaggcac ctaagtctgt
     4021 cattgatttt ggtttatgga ttgccaaagc tcctagccag gaaagaggga gactggcttt
     4081 cttgcagatg cttgctcaaa ccctacaggg ctttcgttaa aactgtcgaa catcagaggg
     4141 aagactctgg tgttttttgc tgatgagact ataaattttg taaaaaatgt gatagaatag
     4201 acgacggata aaataacgga ggatagcatg caaaatagac caatcattat tggagtgaca
     4261 ggtggttctg gtggtggtaa aaccagtgtt tcaagagcca ttttatcgca ttttcctgat
     4321 gaaaagattt ccatgattga gcatgattca tactacaagg atcagtctca tttgaccttt
     4381 gaagagcgtg tcaaaaccaa ctatgaccat ccttttgcct ttgatacaga cttgatgatc
     4441 gagcagatta aggaattgtt ggcagggcgt ccggtggaca tcccgaccta cgactataca
     4501 gagcatacac ggagtagcaa gacctatcgt caggagcctc aagatgtctt tatcgttgag
     4561 ggcattttgg tcttggagga caagcgtctg cgcgatttga tggatatcaa gatttttgtg
     4621 gatacggatg acgatgtgcg tattattcgt cgtatcaagc gtgatatgga ggagcgtggc
     4681 cgtagccttg atagcgttat caaccagtac ttaggtgtgg tcaaaccaat gtaccaccag
     4741 tttatcgagt caactaagcg ttatgctgat atcgtcattc ctgaaggggt tagcaatacc
     4801 gtggctatcg acctgttgac gaccaagatt gcaaagattt tggaagaagc tcgaaacagc
     4861 aaataatcac atgtggaggc cttgcctcct ttttctattt ttccctcata atggtacata
     4921 aatggagatt tttaggcata tttttggtat aataataccc atgaaagagc aagtaaatga
     4981 atagtaggtg gagatggaaa agtatttatc ggtaacaact ttgaccaagt atctgaaaat
     5041 gaaattcgat aaagacccat acttggaacg ggtctattta actggtcaag tttccaactt
     5101 tcgtaaacga cctactcacc aatatttctc cctaaaggat gaccatgcag ttattcaagc
     5161 gaccatctgg tctaggattt atcagaaatt agggtttgac ctggaagaag gaatgaagat
     5221 caatgtgatt gggcgtgtac aggtctatga accaagtggt agctactcca tcatcattga
     5281 aaaagctgag cctgatgggg ttggggcgct tgcgattcag tttgaacaac ttaagaaaaa
     5341 attgacagaa gaaggcctgt ttcaagaacg cttcaagcaa gctctgcccc aattttctaa
     5401 gagaattggt gtagtaacca gccgtagtgg agccgttatt cgagatatta tcacgaccgt
     5461 cagcaggcga ttcccaggtg ttgacatcct tctttatccg accaaggttc aaggtgaagg
     5521 ggctgcggag gaaattgcta gaaatattgc gcgtgctaat caacgggacg atttggattt
     5581 gctcattatt ggtcgtggag gtggttctat tgaggatctc tgggccttta acgaagaaat
     5641 tgtggtacga gctatttttg aatctcgttt gccagttatc tctagtgtgg ggcatgagac
     5701 ggatgtgacc ttggcagatt ttgtggcaga tcgacgcgct gcaacgccaa cagcggcggc
     5761 tgaactggca acacctgtga ccaagttgga tgtattagct catttgcaaa atcaggaaaa
     5821 acggatggta acggcagtcc gaaatgttct atctaagaaa caagaggctt tgaaaaaatg
     5881 cagtcagtct gttatcttta gacaacctga gcgcttgtat gacggttatt tgcaacgctt
     5941 ggaccaactg caactgcgtt tgaaacaaag tttgcgaact cggatttctg ataacaaaca
     6001 attagttcaa gcaagaactc atcaattagt acaattatca cctgttacca aaatccaacg
     6061 ctatcaagac cgtttaggac agttggacaa gctcttaggt agccaaatgg cgttagttta
     6121 tgacgccaag gttgctgagg ccaagcgact ttcggaagct ttgctcatgt tggatactag
     6181 ccgaatcgtg gcgcgtggtt atgctattgt caaaaaagaa gagtccgttg tagattcggt
     6241 tgagagtttg aagaaaaaag accaagtaac gcttttgatg cgagatggtc aagtagaatt
     6301 agaggttaaa gatgtcaaaa caaaagaaat ttgaggaaaa tctagcagaa ctggaaacca
     6361 ttgtccaaag tttggaaaat ggtgaaattg ctctggaaga tgcgattact gcctttcaaa
     6421 agggcatggt cttgtcaaaa gagctccaag ctacgctgga caaggctgaa aagaccttgg
     6481 tcaaggtcat gcaagaagac ggaacagaaa gtgattttga atgaaaaagc aagaaaaatt
     6541 agctcttgtc gagtcggctt tggaagattt ttatggagac cagcagtttg cctctagttt
     6601 acgggagtct gttctctatt ctattcatgc tggtggcaag cgtattcggc cttttctctt
     6661 gttagaagtt ctggaagcct tgcaggttac catcaaacct gctcacgcgc aggtagctac
     6721 tgccttggag atgattcata cagggagctt gattcacgat gaccttcctg ctatggatga
     6781 tgacgattat cgaagagggc ggttaaccaa tcacaagaaa ttcggtgaag ctatggccat
     6841 tttggctgga gatgccttat tcttagaccc atatgccttg attgcgcagg cagatttgcc
     6901 aagtcagatc aaggtgggct tgattgccaa cttatccctt gcttcaggta gtctgggtat
     6961 ggtggcaggg caagttttgg atatggaggg cgaacaccag cacttgtctc tggaagaact
     7021 tcagactatt catgccaata agactgggaa gttactagcc tatcccttcc aagcggcagc
     7081 tattatagct gaattgtcac ctgaaatgca ggtgaagctg aaaactgtgg gtgaattgat
     7141 tggacttgct tttcaagtca gagatgatgt actggatgtg acggctagtt ttgaggaaat
     7201 cggcaagaca cctcaaaagg atctgcaggc agaaaaatca acctatcctg ccttgttggg
     7261 cttggaagag tccattgcct tttgtaacca aaccctggat caagctaatg aaaaattaga
     7321 agaaattgcc cagcagcttc cctttgaaac agaatcgatt gtaagtgtag tagaaagttt
     7381 gagaatcaat ggctaaggaa agagtggatg tactagctta taaacagggg ttgtttgaaa
     7441 cgagagagca ggccaagcga ggtgtgatgg ctggcctagt cgtagcagtc cttaatggag
     7501 aacggtttga caagccagga gagaaaattc cagatgacac cgaattaaaa ctcaaggggg
     7561 agaaactcaa gtatgtcagc cgtggtggtt tgaaactgga aaaggccttg caggtctttg
     7621 atttgtcggt ggatggcgcg actacgattg atatcggggc ctctactgga ggttttaccg
     7681 atgtcatgct acagaatagt gccaagttgg tctttgcagt cgatgttggt accaatcagt
     7741 tggcttggaa attacgccaa gacccacgag ttgtcagcat ggagcagttc aatttccgct
     7801 atgctgaaaa gactgatttc gagcaggagc cgagctttgc cagtattgat gtgagtttca
     7861 tttcccttag tctgattttg ccagccttgc accgtgtctt ggctgatcaa ggtcaggtgg
     7921 tagcacttgt caaacctcag tttgaggcag gacgtgagca gattgggaaa aatggaatta
     7981 ttcgagatgc taagattcat cagaatgtcc ttgaatctgt aacagctatg gcagtagagg
     8041 caggtttttc agtccttggc ttggactttt ctcccatcca aggtggacat ggaaatattg
     8101 aatttttagc gtatttgaaa aaagaaaagt cagcaagcaa tcagattctt gctgagatta
     8161 aagaagcagt agagagggcg catagtcaat ttaaaaatga ataaaaaaga gagacttgaa
     8221 aaaattagaa gattggtgac agattatcaa atcggcacgc aagaagaaat tgtagaacat
     8281 ttgaaagaag caggtatcac tgccactcag gcgacggtat cccgagatat caaagagtta
     8341 ggtattgtca aaattccttt gagagacaac acctatgtct acgaattgcc aaaatcaatc
     8401 gtaaaaagtc tgcaactggc tgaagacaat atcgaatcgg ctgaattgat ggataagatg
     8461 atcaatctcc aagttattcc aggaaatacg gcttttgtaa aagctcagtt aatcgagact
     8521 tttgcagaca agatttttag ctgtttgact gatgatagct cgattttagt cattgccaga
     8581 agtggaagtc tagcagagga aatctttgaa caagtaaaaa attggtaggt ctatatgtta
     8641 cttgaaattt cgataaaaaa ctttgccatt attgaggcta tttccctcaa ttttgaaaag
     8701 gggatgactg tcctgactgg tgaaacgggt gcagggaagt cgattatcat tgatgccatg
     8761 aatatgatgt tgggagctcg tgcgacgaca gatgttattc gtcatggtgc accaaaggca
     8821 gagattgagg ggcttttctc agttgagaat agtcgtcttt tacaggaaat ttttgatgag
     8881 caaggtttag aattaggtga tgaaattatc atccgtcggg aaatcttgca aaatggtcgt
     8941 agtatcagcc gtgtaaatgg tcagatggtt aatctgtctg ttttgcgagc tattgggcaa
     9001 catcttgtag atattcatgg tcagcatgac catgaggagt taatgcgtcc ccaactgcat
     9061 atccagatgt tggatgaatt tggtgatgcc gctttttggg acttgaaaga aacctatcaa
     9121 acgagttttg atgcctatcg gaaaatgcgc aagcaggttc tggaagtcaa gaaaaaccaa
     9181 caggaacaca aggcacgtat cgaaatgttg gaatttcaaa tggcagagat tgaggcagca
     9241 aacttgcagg ctggagaaga cttggctctc aatcaagagc gagataaact cctcaaccac
     9301 aaaaatattg cggatacact gaccaatgcc tacagtatgt tggacaatga agatttttca
     9361 agtctggcca atgttcgttc agctatgaat gacatggaaa gtgtcgaaga gtatgatcct
     9421 gaataccgtg aaatttcaag ctctctgtct gagacctact atgttttaga agacattagc
     9481 aaacgtttgg aagctatcat tgaggacctt gattttgatg gcaatcgcct gatgcaggtt
     9541 gagaatcgtt tggacctcct tcatactatt actcgtaagt atggtgggac tgttgatgat
     9601 gttttgcttt attttgccaa gattacggaa gaatacaatc tcttgacagg caataatctt
     9661 tcgtctgagg acatggaagc agagcttaag aagttggaag tcaatcttgt caatttggca
     9721 ggtcaacttg cttctgctcg tcataatttg gctcagcaac tcgaagctga gattaaacaa
     9781 gaactgcaag atctttatat ggaaaaagcc cagtttcagg ttcgttttag taagggaaaa
     9841 ttcagtcgtg agggaaataa aatggttgag ttttatattt caaccaaccc tggagaagac
     9901 tttaaaccct tggttaaggt tgcttctgga ggggaattat ctcgtctcat gttagccatt
     9961 aagtctgcct tttcacgtaa agaaggcaag actagcattg tctttgatga ggtggatacg
    10021 ggagtttcag gtcgtgttgc tcaagctatt gctcagaaaa ttcataaaat tggtcagcat
    10081 ggtcaggttt tggctatctc ccatttgcca caagtaattg cgattgcaga ttatcaattc
    10141 tttattgaga agattagtaa tgaccattca acggtttcga ctgttcgtct cttgacggtc
    10201 gaagagcgag tggaggaagt tgccaagatg ttggcaggtg atgatgtgac agaagcagcc
    10261 ctgacgcaag ccagagaatt gttgagaaac agggagaaat aagatgacag attattatgt
    10321 aattggagat gttcacggaa aagctgggat gctggaagac cttctcaaaa cctgggatgg
    10381 tcagccccag ttgctctttc taggtgattt gattgacaga ggtgaggata gtcaccgtgt
    10441 tcttgaaatg gtcaaggact tggtggacaa tcaaggggct atctgtttgt ccggaaacca
    10501 cgagtatatg tttttgactt ggctagatga cccagaagaa agttatgacc attatagtcg
    10561 caatggtgga gatacaacca ttaactctat cctaggtcgt cccttggatg caccagttga
    10621 tggagtagaa gatgccaagc gggttgccgc tgaagcgtca gacttggtcg aattcattcg
    10681 tcaaatgcca tttgtagtag agacagacaa gtatatattt gttcacgcag gtattgattt
    10741 gactttggac gactggcatg aaacaacaga ttataaaaaa gtatggctta gaaaaccatt
    10801 ccacggagct gaaaatcata ctggaaaaac cattgtcttc ggtcatacac cggtctatgg
    10861 tttgttacag caagaacgcg gtacatctga gctttggact acagaagatg gcaagattgg
    10921 aatggatgga ggagctgttt atggtggtgt ccttcacggg attgtcttta cagaccaagg
    10981 aatgacagaa caccacttta tcgaaaatga cggctttgtc gctgaagatt agtactccta
    11041 gcagggtatg gtcttgtcaa aatatcaaaa acaatttata ataaatagat accctgaaag
    11101 gaagagaatc atgaacttag aagaattgaa aaaacgacag gggaagatcc gtaacttctc
    11161 tattatcgcc catattgacc acgggaaatc aactctagca gaccgcattt tggaaaaaac
    11221 agagacggtt tcaagtcgtg aaatgcaggc ccagcttttg gatagcatgg atctagagcg
    11281 ggaacgtgga attactatta agttgaatgc catcgagttg aattacactg caaaagatgg
    11341 ggaaacttat attttccact tgattgacac accagggcac gttgacttta cctatgaagt
    11401 ttcacgttcg ctagctgcct gtgagggtgc tattttggtg gtcgatgcgg ctcaaggaat
    11461 tgaggctcaa actcttgcca acgtttatct ggctttggat aatgatttgg aaatcatgcc
    11521 tatcattaat aaaattgacc tgccggctgc agatccggag cgcgtgcgta cagagattga
    11581 agatgtgatt ggtttggatg ccagtgaagc agttttggct tctgccaagg ctggtattgg
    11641 gattgaagaa atcctcgagc aaattgtaga aaaagtacca gcaccaacgg gtgatgtgac
    11701 ggcgccactt aaggccttga ttttcgactc tgtttacgat gcttaccgtg gggttatcct
    11761 ccaagtgcgt gtcatggacg gagtggtcaa acctggtgat aagattcagc tcatgagcaa
    11821 tagtaagacc tttgatgtgg ccgaagtcgg tatttttaca ccaaaagcgg ttggtcgtga
    11881 tttccttgcg actggtgatg ttggttacat tgcggcgtct atcaagacgg ttcaggatac
    11941 tcgtgtgggt gataccgtta ccttggcaac caatcctgcg gcagaaccat tacatggtta
    12001 taagcagatg aatcctatgg tctttgcggg tctctaccca atcgaatcaa acaagtacaa
    12061 tgacctgcgt gaagcgcttg aaaaattgca actgaatgat gctagtcttc agtttgaacc
    12121 agaaacatct caggcacttg gatttggttt ccgttgtgga tttcttggac ttctccatat
    12181 ggatgttatt caggaacgtt tagagcgtga gttcaatatt gacctcatca tgacagctcc
    12241 gtctgttatt tacaaagtta atttgaccga cggtgagtct atggatgtgt ctaacccatc
    12301 tgagtttcca gacccaacta agattgcgac cattgaagag ccttatgtca aggcgcaaat
    12361 catggtacca caggagttcg tcggagcagt aatggagcta gctcagcgta agcgtgggga
    12421 ctttgtgact atggactata ttgatgataa ccgtgtcaat gttatctatc aaattcctct
    12481 tgctgaaatt gtctttgact tctttgataa acttaagtct tcgacacgtg gttatgcaag
    12541 ctttgactac gaattgtcag aatatcgccc atctaagctg gtgaaaatgg atatccttct
    12601 caatggagat aaggtggatg ccctcagctt tatcgttcac aaggactttg cctacgaacg
    12661 tgggaaactc atcgttgata aactcaagaa aatcatccct cgccaacaat ttgaagttcc
    12721 aatccaagcg gctattggac acaagattgt cgctcgtact gatatcaagg cccttcgtaa
    12781 gaacgtactt gctaaatgtt atggtggtga cgtttctcgt aagcgtaaac tccttgaaaa
    12841 acaaaaagct ggtaagaaac gcatgaaatc catcggatca gttgaagttc cgcaagaagc
    12901 cttcctcagc gtcttgagta tggatgaaga ataagttatc agaaatgcct ttccttgttc
    12961 acaggaaatt tatataaaaa tcaatagtaa agcaggctta gatggtctgc ttttttagtt
    13021 tttatactct tcgaaaatct cttcaaacca cgtcagcgtc gccttaccgt atgtatggtt
    13081 actgacttcg tcagtttcat ctacaacctc aaaactgtgt tttgaacaac ctgcggctag
    13141 ctt
//
LOCUS       DAVKAO010000045        12852 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKAO010000045 DAVKAO010000000
VERSION     DAVKAO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.045"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            311..871
                     /locus_tag="VWX16_001431"
     CDS             311..871
                     /locus_tag="VWX16_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9158765.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLSFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            872..2257
                     /locus_tag="VWX16_001432"
     CDS             872..2257
                     /locus_tag="VWX16_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158766.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHVQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIARKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTSIVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            2259..2909
                     /locus_tag="VWX16_001433"
     CDS             2259..2909
                     /locus_tag="VWX16_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9158767.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQFKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            2920..3612
                     /gene="tenA"
                     /locus_tag="VWX16_001434"
     CDS             2920..3612
                     /gene="tenA"
                     /locus_tag="VWX16_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9158768.1"
                     /translation="MEFTYIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            3617..4141
                     /gene="thiW"
                     /locus_tag="VWX16_001435"
     CDS             3617..4141
                     /gene="thiW"
                     /locus_tag="VWX16_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9158769.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            4141..4944
                     /locus_tag="VWX16_001436"
     CDS             4141..4944
                     /locus_tag="VWX16_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9158770.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAALYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            4937..5569
                     /locus_tag="VWX16_001437"
     CDS             4937..5569
                     /locus_tag="VWX16_001437"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9158771.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(5778..6569)
                     /gene="thiD"
                     /locus_tag="VWX16_001438"
     CDS             complement(5778..6569)
                     /gene="thiD"
                     /locus_tag="VWX16_001438"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9158772.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(6653..6751)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6935..7330
                     /locus_tag="VWX16_001439"
     CDS             6935..7330
                     /locus_tag="VWX16_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9158773.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            7341..7712
                     /locus_tag="VWX16_001440"
     CDS             7341..7712
                     /locus_tag="VWX16_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9158774.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            7722..9965
                     /locus_tag="VWX16_001441"
     CDS             7722..9965
                     /locus_tag="VWX16_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9158775.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEAIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLVISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            10172..11947
                     /gene="spxB"
                     /locus_tag="VWX16_001442"
     CDS             10172..11947
                     /gene="spxB"
                     /locus_tag="VWX16_001442"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9158776.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            12058..12405
                     /locus_tag="VWX16_001443"
     CDS             12058..12405
                     /locus_tag="VWX16_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9158777.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(12522..12852)
                     /locus_tag="VWX16_001444"
                     /pseudo
     CDS             complement(12522..12852)
                     /locus_tag="VWX16_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3660 a         2462 c         2853 g         3877 t
ORIGIN      
        1 tgaacctgat acagttaaga ctggcgaagg gaaatgtgaa atgttttttt cgtatgtcga
       61 aaagaactgt ctaatcttgt aagctgaact ctaattcgta tctgtaatta gagttttttc
      121 ttttacgaaa ctggatgaag atgatatagt agattgaaat aagatgcgaa caaatcaatt
      181 agaaaattca aattaatttc tagaaatatt ttagcattct tgtgtactgt tctaaattca
      241 gtctgctata caaagtattt aggtcggtct tcttgagtgc cccacttttc tttaaaaaaa
      301 ggagtttttt atgttgaaaa aatggcagtt aaaagatgtt atcttgcttg ctttcttgtc
      361 tatctttttt ggtggggttt tcgttggttc aggatatgtg tataatattc tcagtctact
      421 cttaacacct cttggtttgc aggcctttgc caatgaaatc ctcttcggtc tctggtgtat
      481 ggctgcgccc attgctgcca tctttgttcc gagagtcgga agtgcaacga ttggagaagt
      541 gctagctgcg cttgctgaag tcctttatgg tagccaattt ggtctaggag ctcttttgtc
      601 tggcttggtt caaggtttgg gaagtgaatt tggttttatc gtaactaaga atcgctatga
      661 aagttggctc tctctaactg ctaatagtat tgggattacg cttgttagct ttgtctatga
      721 atacattaag ttaggttact acgccttttc cctttcgttt gtcctttcct tgcttgtggt
      781 acgttttatt tctgtttatt tcttctgtac catcttggtt cgtgccattg tcaaactcta
      841 tcatcagttt gcaactggag gaaaagcata gatgggtctg gaactacgag cgattcagtc
      901 cccaatcttc tctgagccgt ttgattttac ttttcatgtg caagccttta ccttgttagt
      961 tgggagtagt gggtctggaa aatcaagcct ctttcaaatg attgcccaag ttagttctct
     1021 tccctatagc ggtcaagtcc tgatagatgg gagtgaggtc agtcagcttt ctatcgtcga
     1081 acgtgtccag aaggttggca ttctctttca aaatcccaat catcaattta ccatggagaa
     1141 cttgtttgag gagttgattt tcaccttgga gaatatcggc tatcaccttc aggaaattga
     1201 ttctaaaata gcagaggttg tccagcaatg tcgttgcgag gcaatcttac accgtcccat
     1261 tcatcaccta tcaggtgggg aaaagcaaaa agcagcgctg gctgtcctct ttgccatgaa
     1321 tcctagggtc tatctcttgg atgagccctt cgcttctatt gcccgcaaga gcagaatcga
     1381 gatattggag attctaaaag agttggctct tgatgggaag acagttattt tgtgcgacca
     1441 tgatttatct gactataaag cctatatcga ccatatggtt gagctaagag acggaaaact
     1501 aagggaagtg tttcaaatcc cttcctatga gatgacacag gttgcttcaa aggaagttgc
     1561 ttctagcccg gaactattcc atatgaaccg tgtgactggt gagcttggta atcgccccct
     1621 cttttcaatt gctgatttca cattctatca agggatttcc tgtatcctgg gtgacaatgg
     1681 tgtcgggaaa tcaaccctct ttcggtctat tcttcaattt caaaagtata aggggcgcat
     1741 tgcatggaag gggacagtcc tgaaaaagaa aaagagtttg taccgtgatc tgacgagtat
     1801 tgttcaggaa gctgagaagc agtttatccg agtcagtctg cgagaggagc ttcaattaga
     1861 tggacctgat tctgaaagaa atcagcggat ttttcaagct ttacgatatt ttgatttgga
     1921 gcaggcagtc gataagagtc cctatcaatt aagtggtggt cagcaaaaaa ttcttcagct
     1981 cctgaccatc ttgaccagta aggcttccgt gatcttgcta gatgaacctt ttgcaggttt
     2041 ggatgataga gcctgccatt atttttgcaa gtggattgtg gaggagagga atcaaggaag
     2101 aagttttctg ctcattagtc atcgtttaga ccctttgatt tctgtggttg attattggat
     2161 tgagatgact agtcagggtc ttcggcatgt gaaagaagtg accattacca aaccacttac
     2221 atctcagagt agcaataccc aaggggaggt gagatagtat ggtcaaagta gcaacccaga
     2281 caccgattat cagtctcttc ttgctgattt tatccttgga aacatctttc attccttcga
     2341 ttgctctgaa tctttcggta gtcgcatttt gtattctctt tatgctctat taccgtcgat
     2401 ttaaaatgtt agcttggatg atcatacttg ccattttacc atcttttgcc aactactggg
     2461 cagttcagtt acacggagat gcttcacagg cagtcatgct tggaacgagg gcctttgtga
     2521 cagtttgtat cggccttgtc tttgtttcct ctatttcact aaaagagctt ctcttgtact
     2581 tggctcaaaa ggggctatca cgctcttggt cctatgcctt gattgtggta ttcaattcct
     2641 ttcctctcat tcagcaagaa atcaagtccc tcaaagaagc ttgtctatta cgtggtcaag
     2701 aactgcattt ttggtcgccc ttgatttaca gtaaggttct gatgacagtc tttaggtggc
     2761 gccatcttta cctgagagct ctgtctgctc atggatatga cgaacatgca cagtttaaga
     2821 atagctatcg gactttttat attcctaaaa aaacaaaatt aatctacctg cttttctttt
     2881 tattgcttca aaccagtcta tttttataaa ggagttatta tggaatttac atatattgcg
     2941 atggaattat ccaagaaagc ttggcaggct tcctttcatc atccctttat tttacaatta
     3001 caagagggga atttagaacc tgccattttc cgctattacc tgattcagga tgcctactat
     3061 ctgaaggcct tctcagaaat ctatcatctt ttggctgata agacttcaaa ccaagagatg
     3121 aaaagactct tgaaacaaaa tgctcagggt ttagtggagg gtgagttatt tattcgccaa
     3181 caatttttca aggaaatgga aatcagcgac caggaaatgg agcaacatcc aattgctcca
     3241 acctgttatc attacatttc tcatatttat aggcaatttg cagaaccgaa cttagccatc
     3301 gcttttgcaa gcttgcttcc ttgtccttgg ttataccatg atataggcaa atcacttaat
     3361 cttaaaccat caccaaatcc tctctatcaa caatggattg aaacttatat tacggatgag
     3421 ttagagcaac agatcagaga ggagggagca ctggtcaatc agctctatcg agaaagtgat
     3481 gagacagata agcaaaaaat gctagatgcc ttccacatca gtgttcatat ggaagccaag
     3541 ttttgggaga tggcttacca acaccagaca tggaagagcg atttacagtc tttagaaaaa
     3601 ggagaagaat agaaacatga gaaagcacca actacaagtt cacaaattga ccattttatc
     3661 tatgatgatt gcccttgatg tagtccttac acctatcttt cggattgagg gaatggcacc
     3721 gatgtccagt gtagtcaata ttctagcggg aatcatgatg ggacctgttt atgccttggc
     3781 tatggctaca gtgacagcct ttatccgtat gacgactcaa gggattccgc ctttagctct
     3841 cacaggagcg acttttggag cccttctagc aggtctcttt tataagtacg gtcgaaaatt
     3901 ttacttttct gccttgggag aaattgtggg aacaggtatt attggttcta ttgtttccta
     3961 tcctgttatg gtgctcttta caggatcggc tgctaagctt agttggttta tctacacgcc
     4021 tcgatttttc ggagcaacct tgattggtac agcgatttcc tttattgcat ttagattttt
     4081 aatcaagcag gaattcttta aaaaagtgca aggatatttc tttgctgaaa ggatagaatg
     4141 atgcaggcat tgacaaatcc ttttcctata ggctctagtt ccctcattca ctgtatgacc
     4201 aatgagattt cttgtgagat gctggcaaat gggattttgg ctctgggatg caaacctgtc
     4261 atggcagatg atccccgtga agttcttgat tttactaagc aaagccaggc tctcttcatc
     4321 aatttggggc atttgtcagc tgagaaggaa aaagcaatcc gcatggcagc tttgtatgca
     4381 aaccaatctt ctctcccgat ggtagtagat gcggttggcg taacgacttc atccattcgt
     4441 aagagcttag ttaaagacct tttagactat agacctacgg tccttaaagg aaacatgtca
     4501 gaaattcgaa gtcttgttgg attaaagcat cacggcgttg gggtcgatgc gagtgctaaa
     4561 gatcaagaaa cggaggattt gcttcaagtc ttgaaagact ggtgtcagac ctatcctggt
     4621 atgtcattct tagtcacagg tcccaaggac cttattgttt cggagaatca agttgctgta
     4681 cttgagaatg gctgtactga attagactgg ataacaggga caggagactt ggttggagcc
     4741 ttaacagctg tttttctcag ccaaggaaag actggttttg aagcttcttg cttagcagtc
     4801 tcttatctca atatcgctgc tgagaaaata gttgttcaag gaatgggatt ggaagaattt
     4861 cgttaccaag tactcaatca gctttcgctc ctaagaagag atgaaaattg gctagatacc
     4921 atcaaaggag aggtttatga atagagaagc gcttagacta tatctggtaa ccaatcgcta
     4981 tcaagattcc gtggaaagct ttcttgcaaa agttgagacg gcctgccgtt caggggttac
     5041 catagtccaa ttgcgagaaa aaaatctcac aaccaatcaa tattatcaac tggcaaaaca
     5101 agtcaaggaa ataacagatg cctatcaggt ccccttgata atcgatgatc ggttggatgt
     5161 ttgtcttgcg gttgatgctg ctggtctgca tattggagat gacgaactcc cagtttcggt
     5221 tgcccgacaa gtcttgggtc ctgaaaaaat cctcggtgtc accgctaaaa cggttaaaag
     5281 agctctggaa gcggagaaat caggtgcaga ttacttgggg acaggagcca tttttccgac
     5341 aaccaccaag gaaaatgcac ccatcaccct gatttcaacc ttgaaaacaa tttgccaaac
     5401 ggttgccatt ccaatagttg ctattggcgg cttgacttcg gagaatattg atcagcttat
     5461 gggcactggt atagctggtg tagctgttgt acgtgatttg atgcaagcag aagatattga
     5521 ggcaaaaacg caagcctttt taacaaagtt gcatgacatt ctttcctaat taatactcaa
     5581 tgaaaatcaa agagtaaact agaaagctag tcgcaggttg ctcaaagtac tgccttgagg
     5641 ttttagataa gactgacgaa gtcagctcaa aacagtgttt tgaggttgca gatggaagct
     5701 gacgtagttt gaagagattt tcgaagggta taaaaactct ctaattccct taaagtggga
     5761 actagagagt ttttttctta atctttaaaa gttgtatggt tgactggacc agaaccatga
     5821 ccgagttgag gggcatcttg aatagctttt gtgataaagg ccttggcctt atcaactgcc
     5881 tggtaaagac tcttgccctt ggctagttca gcagtaatca ctgcagcaaa ggtacatcca
     5941 gtaccatggg tgtgacaggt ttgaattcgt gggctttccc agacaaattg ttcattcttg
     6001 gtaaagagga aatctttagc accacctttg agatgtccgc ctttgataac cacagactga
     6061 ggaccaaatt cttttaaaat caggcgacca gcacgctgca tgtcttcggg gtcatggatt
     6121 gaaaaaccaa caatctcttc tgcttcagga agatttggcg taataatagt tgctagaggt
     6181 agtaagtttg ttttgagata gtctctagca cttgagtcaa tcaaggcatc tccacttgta
     6241 gcaaccataa caggatcaag gacataggga caatccagtt ttttaagata gggttggatg
     6301 atttccatga tttcagtagt agccaacatt ccagttttta cagcctgagg tggaatatca
     6361 gaaaagacac tctccaattg ggctttcaac atttgaggag aaacgtgctc gattagctga
     6421 acacctctgg tattttgagc gacaagactg gttacaacag ccattccata gacatctctc
     6481 gcttggaatg actttaaatc tgccataatg ccagcaccac cactagggtc agtccctgca
     6541 atggttaaag caacgggtaa ataagtcatc taaaacctcc caaccaactg aagtttgtat
     6601 tcttaagaac aagctagtct gttttagaaa ggcaatagaa gactgctagt ccccattatc
     6661 atttccactt ccatcagcta gcattaccta gattgagtca gagggatcta acagtcgtta
     6721 atctcagctt tacgcacccc tagtggttgt tttctttttt ctttagtata gcatattttt
     6781 attgtttata tagtaatatt tgactgcaaa tcgtttttca aatgattaaa attctatttt
     6841 tcgaagaaaa tattatttct ataattgaca aatgtagatt ttgggagtat actgttgagt
     6901 gtaattgaca aatgtagatt ttggaggtat ggtaatgcag atttcagatg cagaatggca
     6961 ggtcatgaag attatttgga tgcaggggga gcagaccagt acagatttga ttagggtttt
     7021 ggcagagcgg tttgactggt ccaagtccac ggttcagaca cttttggctc gtctagttga
     7081 gaaagagtgt ttgactcgga aaaaagaagg caagtccttt gtttattcag cgcttttaac
     7141 tctagaccaa agtcgggatt tacttgtcca agatatcaaa gacaaggttt gttcccgtag
     7201 gattaggaat ttgttggctg acttgattgt agaatgtgaa tttactcaga ctgacttgga
     7261 agacttggaa gctgtgattt cagagaagaa atcaagtgct gtaacagaag taagatgtaa
     7321 ttgtatgtaa aggagacgtc atgttaaata gtattgtaac cattatttgt attgccctta
     7381 tcgcgtttat cttgttttgg tttttcaaaa agcctgaaaa atctggacaa aaagcccagc
     7441 aaaaaaacgg ataccaagag attcgagtgg aagtcatggg aggctatact cctgagttga
     7501 ttgtcctcaa gaaatcagtg ccagcccgca ttgtctttga ccgcaaggat ccttcaccat
     7561 gtctggatca aattgttttt ccagattttg gtgtacatgc gaacctgcca atgggggaag
     7621 agtatgtagt ggaaatcacg cctgaacagg ctggagagtt tggctttgct tgtggtatga
     7681 acatgatgca cggcaagatg attgtagagt aggtggagac tatgacagaa attgtgaaag
     7741 caagcttaga aaatggcatt caaaaaatcc gtatccgagc tgaaaaaggc tatcatccag
     7801 cccatatcca gcttcaaaag ggaattccag ctgagattac ctttcatcgt gctactcctt
     7861 caaactgtta taaggaaatt ctgtttgaag aagaaggtat cttggaagca atcggcgtag
     7921 atgaggagaa agtcattcgt tttacacctc aagaattagg gagacatgaa ttttcttgtg
     7981 gcatgaagat gcaaaaggga agctatatag tcgttgagaa gactcgaaaa tctctatctc
     8041 tcctgcaacg tttttggatt actagtatct ttactgtgcc tcttgtgatt ctcatgattg
     8101 ggatgttggc aggtagcatt agtcatcaag tcatgcattg gggaaccttt ttagcaacaa
     8161 cgcctattat gttagttgcg ggtaagccat atatccagag tgcttgggcc agttttaaaa
     8221 agcacaatgc caacatggat accttggttg cgctgggaac tctagtggct tatttctata
     8281 gcctagttgc tctctttgct ggtctccctg tttacttcga aagtgctgga tttatcctct
     8341 ttttcgttct tttgggagca gtttttgagg aaaaaatgag gaaaaatacg tcccaagctg
     8401 tggagaaatt actggacttg caagctaaaa ccgcagaagt cttgagtgat gatagttatg
     8461 tccaagttcc tttggaacaa gtcaaggtag gcgacctgat tcgagtgcgt cccggtgaaa
     8521 agattgctgt tgatggtgtc gtagtagaag gtgtctctag tattgacgaa tccatggtga
     8581 caggtgagag tctgcctgtg gacaagacag ttggagatac tgtcattggc tcaaccatca
     8641 atcatagtgg aacgcttgtc tttagagcag aaaaagttgg ctcagagact gttttggctc
     8701 agattgtaga ttttgtgaag aaagctcaga caagtcgtgc gccgattcag gacttgacgg
     8761 ataagatttc agggattttt gtcccagtag ttgtcatttt aggaatcatg accttttggg
     8821 tttggttcgt cttgctcagg gatagtgtgg tcgtgcttgg agctagcttt gtgtcctctc
     8881 ttctctacgg agtggcggtt ttgattatcg cctgtccttg tgccttggga cttgcaacac
     8941 cgacagccct tatggtgggg acaggacgta gtgccaagat gggggttctc ctcaaaaatg
     9001 gaactgtctt acaggaaatc cagaaagttc aaactcttgt ctttgataag accgggactt
     9061 tgacggaagg gaaacctgtg gtaacagata tcatcggcga cgaagtagaa gtgtttgggt
     9121 tggcagcctc cttggaagat gcttctcaac acccactggc tgaggctatc gttaagcgag
     9181 cgagtgaagc tggacttgag tttcaaactg ttgaaaattt tcagaccttg cacgggaaag
     9241 gtgtttcagg gcgaattaat ggaaaacaag ttttacttgg aaatgctaaa atgctggatg
     9301 gcatggatat ttctaatact tatcaagata aactagaaga actagaaaaa gaagctaaga
     9361 cagttgtgtt cttagctgtt gacaatgaaa tcaaaggttt gcttgctttg caagatattc
     9421 ctaaggaaaa tgctaagcta gtcatcagtc agctaaaaaa acgtggtctc cgaacagtca
     9481 tgctgacagg agacaatgct ggtgtggcgc gtgctattgc agatcaaatc ggaattgaag
     9541 aggtcattgc aggcgtcttg ccagaagaaa aagcccatga aatccataaa ctgcaacagt
     9601 caggtaaagt agcctttgtt ggtgatggga tcaatgacgc tcctgccctt agtgtagcag
     9661 atgtggggat tgctatggga gctggaacag atatcgccat cgagtcagca gatttggtgt
     9721 tgacaaccaa taatctttta ggagtggtgc gtgcctttga tatgagtaag aaaacctttc
     9781 atcgaattct actcaatctt ttctgggctt ttatctacaa tgttgtcgga attccgattg
     9841 cagcaggagt cttttcaggt gttggactgg ctctcaaccc agaattggca ggtctagcca
     9901 tggcctttag ttctgtatct gttctgacca gttcccttct gttaaatttt agtaaaatag
     9961 actaaaagta atgactagat ttctttgtta taaaacaaaa atgacaacac cttcaaaact
    10021 gtattagaat agagaataga gagttttgag cagattttta gaaaagtcag cataaatatg
    10081 atacagtgga atagtaaaaa tttggagaac gtttccaatt ctatgtaatc gtattctcca
    10141 agtctaaaaa aattgaagga gagttatcat tatgactcaa gggaaaatta ctgcatctgc
    10201 agcaatgctt aacgtattga aaacatgggg cgtagataca atctacggta tcccatcagg
    10261 aacactcagc tcattgatgg acgctttggc tgaagacaaa gatatccgtt tcttacaagt
    10321 tcgccacgaa gagacaggtg ctcttgcagc ggttatgcaa gctaaattcg gcggctcaat
    10381 cggggttgca gttggttcag gtggtccagg tgcgactcac ttgattaacg gtgtttacga
    10441 tgcagctatg gataacactc cattcctagc gatccttgga tcacgtccag ttaacgaatt
    10501 gaacatggat gctttccaag agcttaacca aaacccaatg tacaacggta tcgctgttta
    10561 caacaaacgt gtagcttacg ctgagcaatt gccaaaagta attgacgaag cctgccgtgc
    10621 tgcagtttct aaaaaaggtc cagctgttgt tgaaattcca gtaaacttcg gtttccaaga
    10681 aatcgatgaa aactcatact acggttcagg ttcatacgaa cgctcattca tcgctcctgc
    10741 tttgaacgaa gttgaaatcg acaaagctgt tgaaatcttg aacaatgctg aacgcccagt
    10801 tatctatgct ggatttggtg gtgttaaagc tggtgaagtg attactgaat tgtcacgtaa
    10861 aatcaaagca ccaatcatca caactggtaa aaactttgaa gctttcgaat ggaactatga
    10921 aggtttgaca ggttctgctt accgtgttgg ttggaaacca gccaacgaag tggtctttga
    10981 agcagacaca gttcttttcc ttggttcaaa cttcccattt gctgaagttt acgaagcatt
    11041 caagaacact gaaaaattca tccaagtcga tatcgaccct tacaaacttg gtaaacgtca
    11101 tgcccttgac gcttcaatcc ttggtgatgc tggtcaagca gctaaagcta tccttgacaa
    11161 agtaaaccca gttgaatcaa ctccatggtg gcgtgcaaac gttaagaaca accaaaactg
    11221 gcgtgattac atgaacaaac tcgaaggtaa aactgagggt gaattgcaat tgtatcaagt
    11281 ttacaatgca atcaacaaac atgctgatca agacgctatc tactcaatcg acgtaggtaa
    11341 cactactcaa acatctactc gtcaccttca catgacacct aagaatatgt ggcgtacatc
    11401 tccgctcttt gcgacaatgg gtattgccct tcctggtggt atcgctgcta agaaagacaa
    11461 tccagatcgc caagtatgga acatcatggg tgatggagca ttcaacatgt gctacccaga
    11521 cgttatcaca aacgttcaat acgaccttcc agttatcaac cttgtcttct caaatgctga
    11581 gtacggcttc atcaagaaca aatacgaaga tacaaacaaa cacttgtttg gtgtagactt
    11641 cacaaacgct gactacgcta aaattgcgga agctcaagga gctgttggat tcacagttga
    11701 ccgtatcgaa gacatcgatg cagttgttgc agaagctgtt aaattgaaca aagaaggtaa
    11761 aactgttgtc atcgatgctc gcatcactca acaccgtcca cttccagtag aagtacttga
    11821 attggatcca aaacttcact cagaagaagc tatcaaagcc ttcaaggaaa aatacgaagc
    11881 agaagaactc gtaccattcc gtctcttctt ggaagaagaa ggattgcaat cacgcgcaat
    11941 taaataattc ctctcgccga aaatcaaata tgaaacttgt agactttatt ctttgatttt
    12001 catagagcag aactttaaaa gatcggagca atccggtctt tttatggagg agagtaaatg
    12061 aatttaaatc aattagatat tatcgtttca aatgttcccc aagtctgtgc tgacttggag
    12121 catattttgg ataaaaaggc agattatgct gatgatggtt ttgctcagtt tacgattggt
    12181 agtcactgcc ttatgttgtc acaaaatcat ttggttcctt tggaaaactt tcagtcagga
    12241 atcattattc atatcgaggt tgaggatgta gaccagaact acaaacggtt gaacgagctt
    12301 ggtataaagg ttttacacgg accaactgta accgattggg gaacagagtc cttattagtt
    12361 caaggacctg ctggtctagt gcttgatttt tatcgtatga aataggtcgc gtagtcattt
    12421 gacgttaacc ttagctattt ttaaaataga aattgagaat atgaactatt gaaagatcgg
    12481 agcaatccgg tctttttata taaagaaatt gcatataata ctcatctgct ttcaaaaagc
    12541 attctagtcc atctccgatt aacgatggac tttatcacct ccttctccag tccttgtatg
    12601 acatcttgga gttgattcat gacctcttcc aatatgggca tcaacagctc cataacaata
    12661 gcgaaattct cgtatatagt gactatggat atgtggacct actcctattg gagcccaaca
    12721 agatcccagt ttactgattc taccgaaacc agcctcatct tggtacatca agcgaacctt
    12781 acgaaaacgt ctactggttt taaagcgctt tcttgtcttc ttgaattgag attttatttt
    12841 tagacgcgac aa
//
LOCUS       DAVKAO010000046        12400 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKAO010000046 DAVKAO010000000
VERSION     DAVKAO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.046"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            442..735
                     /locus_tag="VWX16_001445"
                     /pseudo
     CDS             442..735
                     /locus_tag="VWX16_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            842..1133
                     /locus_tag="VWX16_001446"
                     /pseudo
     CDS             842..1133
                     /locus_tag="VWX16_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1480..2786
                     /locus_tag="VWX16_001447"
                     /pseudo
     CDS             1480..2786
                     /locus_tag="VWX16_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2826..3836)
                     /gene="tsaD"
                     /locus_tag="VWX16_001448"
     CDS             complement(2826..3836)
                     /gene="tsaD"
                     /locus_tag="VWX16_001448"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9158778.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVLDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEVTDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFANLDLNAKPSLAFDT
                     ME"
     gene            complement(3826..4263)
                     /gene="rimI"
                     /locus_tag="VWX16_001449"
     CDS             complement(3826..4263)
                     /gene="rimI"
                     /locus_tag="VWX16_001449"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9158779.1"
                     /translation="MIEIKRIQQQPDLAKAIYAVMIAVYPVSPWTLEQIQADLFQDQT
                     WYALAYDGAEVIGFLAVQETLFEAEVLQIAVKGAYQGKGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4260..4943)
                     /gene="tsaB"
                     /locus_tag="VWX16_001450"
     CDS             complement(4260..4943)
                     /gene="tsaB"
                     /locus_tag="VWX16_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9158780.1"
                     /translation="MKVLAFDTSSKALSLVILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPR
                     QQEGLVVPIMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5121..5306
                     /gene="cibA"
                     /locus_tag="VWX16_001451"
     CDS             5121..5306
                     /gene="cibA"
                     /locus_tag="VWX16_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9158781.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5308..5460
                     /gene="cibB"
                     /locus_tag="VWX16_001452"
     CDS             5308..5460
                     /gene="cibB"
                     /locus_tag="VWX16_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9158782.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            6266..6499
                     /locus_tag="VWX16_001453"
     CDS             6266..6499
                     /locus_tag="VWX16_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9158783.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLNIDASSELEGRIAARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6501..8180
                     /locus_tag="VWX16_001454"
     CDS             6501..8180
                     /locus_tag="VWX16_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9158784.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     ICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEILILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8514..10427)
                     /gene="mnmG"
                     /locus_tag="VWX16_001455"
     CDS             complement(8514..10427)
                     /gene="mnmG"
                     /locus_tag="VWX16_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9158785.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10437..10892)
                     /locus_tag="VWX16_001456"
     CDS             complement(10437..10892)
                     /locus_tag="VWX16_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9158786.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(11033..12154)
                     /gene="mnmA"
                     /locus_tag="VWX16_001457"
     CDS             complement(11033..12154)
                     /gene="mnmA"
                     /locus_tag="VWX16_001457"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9158787.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3530 a         2912 c         2449 g         3509 t
ORIGIN      
        1 ctctagatac gattatcaaa ataggatata gagacatcct tctgatctgc tttttacaaa
       61 gtccaattat atgcggatct atacctccac aatgtccatt attataccta actataatat
      121 gagccgaaaa cactatatcc ttaatgtctc catatccatc agggatatta atatttattt
      181 ttccacaact atattgcatt gtaaccatct ccttaaacga cgcattatga tatttgatag
      241 agaaattttt atgaataact caataatttt atagtaaatc atgcttatat ctcaaagata
      301 cctattttat cttgtctcga ccttctccaa agaattgcta taatactatt acaaatacat
      361 ctgcactaca cttcaaattt tagcactgta taaaaacgtt tcaatacact aacttcaaga
      421 aaacttccac tattaattga aaaaattgat agagataaat taaagatcta tattgaaact
      481 catcccgatg cttatttgac tgaaatagct gctgaattca actgtcctcc aacaactatt
      541 cattacgctc taaaggctat gggatatagt ctaaaaaaga gccgtaccta ctgcgaacaa
      601 gacccagaaa aagtaaatcg gttccttaaa gaattgaatc acttaagcta cctgactcct
      661 atttatattt atgagacagg ggttgagacc tatttttatc tcgaatatga tcgagccttg
      721 agcaggcagt tagtctctct ggaagaagat ataattattt gaattaagat cgagacaacg
      781 cacaccagag attgcgatac tgttatagaa gtactaatgc ccttttttgt ttcaatatac
      841 tatggctccg atgacctata aagatacgat gacgagtgac tttttcgaag cttgcttcca
      901 aaaattctta ctacctactt tagatacacc atcccttatc attatggaca atgcaaggtt
      961 tcacagaatg aacatgtgta aggagcaggg catagactgt taccacttcc tacctattca
     1021 cccgagtata atcccattga gaaaatatgg gcttacatca aaaacatctc agaataatat
     1081 tgtcaaatta cgatgctttt cttgaggcac ttttgtccta ttcttgtttc agccgactat
     1141 actccgttat tggacagcta cggaacagtc gatgggacga tggggggacg atggggggac
     1201 ataaaaaaat cctccagttt tgttttttat aacagtatac tggagaattg acaatctcgg
     1261 tagatacctc gttatagcgc ggttacttat taggcagtta caaaacaact gtgaacagaa
     1321 aacattccag agtcagacaa gactttggaa tgttttggct ctataatttc tgtagtgggt
     1381 aatcccaccc caggaattat agggtcgttt cttgtagaaa aaaagcccca tatgacctat
     1441 aatgaaaagc gtctaaccaa ctcattagaa aggggtcata tggaacaact taagaatacc
     1501 acagatttgc tcggattgga agacaaaaat atcaaaatct tgtctgttct gaaataccaa
     1561 acccatctag tcgttcaggc aaagttggat tcccccgctc ctccttgtcc tcattgtcaa
     1621 gggaagatga tcaaatacga cttccagaaa gcctctaaaa ttccgcttct cgactgtcag
     1681 ggtttaccca cggtactgca tctcaaaaag cgccgctttc agtgcaagaa ttgccttaag
     1741 gtggtcgttt ctcaaacatc cattgtcaag aaaaattgcc agatttccaa catggtgaga
     1801 caaaaaatcg ctcagctcct ccttgaaaag cagtctatga ctgagattgc ccacagattg
     1861 gcggtctcaa cttccaccgt catccgaaaa ctgagggaat ttaagtttga aaccgattgg
     1921 accaagttgc caaaagttat gagttgggat gagtatagct tcaaaaagag caaaatgagc
     1981 ttcattgccc aagattttga gtccaaatcc atcctcgcaa ttttagacgg gcgaactcat
     2041 gcggtgattc gaaaccattt ccaacgctat cagagagagg ttcgggagct ggtcgaggtc
     2101 atcaccatgg acatgtacag cccttattat cggctcgcta agcaactctt tccaaaggcg
     2161 aagattgttc ttgaccgctt ccacattgtc caacatctga gccgagctat gaaccgagta
     2221 cgaatccaaa tcatgaacca atttgaccga aaatccttgg agtatcgggc gctcaagcgc
     2281 ttttggaacc ctcgcttttt cgtttctagg ctcgggctaa atcagtccac tggactgatt
     2341 tactacacca ggatagcttc aagctctgtc agaaacgatt ctatcagccc acgtttcgaa
     2401 tgcacttaac ccatcgggaa gtacgagata aactgctttc ttactctgag ggattacagg
     2461 ttcactacga actctatcaa ctcctgctct ttcactttca agagaagaat gccgaccatt
     2521 tctttggatt gattgagcaa gaactgccaa cggttcatcc gctttttcaa acggtctttt
     2581 ggactttttt aagggataga gataagatta tcaacgcact taagctgcct tattccaacg
     2641 ctaaacttga agcgaccaat aatttgatta aggttatcaa gcgcaaagcc tttggtttcc
     2701 ggaactttaa caattttaaa aaacggattt tgatgacttt gaacatcaaa aaagagagta
     2761 cgaatttcgt actctccaga ttgtagcttt tcgcctaccc actacacttg acaaagagcc
     2821 actctttatt ccatggtatc aaaagcgagg cttggtttgg cattgaggtc caagtttgca
     2881 aagttttctt tgttccactc gctgacactg gcataagcaa tcatacctgc attgtctccg
     2941 cagaggcgca gaggtggaat gatgaccttg acatctgtaa cctcggctgc taggcgttct
     3001 ctgagacctt tattggctgc cacaccacct gccacaacca gggttttaac aggatatttc
     3061 tccaaagcct tcttggtttt tgccatgaga atatccagta cagctgcttg gaaggaagca
     3121 cacaaatcct ctgtagacag actttctccc ttttgctcgg cattgtggtg aagattgata
     3181 aaggcagatt tcaaaccaga gaatgaaaac tccagattat cttccttaat catagcacga
     3241 gggaaatcat aaatatcctg cccctgatga gccagctcgt caatctcacg acctgcagga
     3301 taggtcaagc ccatgacacg accgaccttg tcataagcct ccccaactgc atcgtctcgt
     3361 gtctctccaa caatcttgta atcgccagcc tcagaaacat agaccaactc tgtgtgccca
     3421 ccactgacta aaagggctag caagggaaac tccaaaggct ccacactctg agctgccatg
     3481 aggtgcccag ccatgtgatt aacaggaatc agtggaagtc cgtgagccca agcaaaggcc
     3541 ttggcggctg acaaaccaac tagcaaggct ccgaccaagc ctggtccgta ggtaaccgca
     3601 acagctgtca cgtcctcttc ggtaatccct gcttctgcca atgcctcctc gatacaggct
     3661 gtaatgacct cgacatggtg acgactggct acttcgggca ctacgccacc aaaacgtttg
     3721 tgactctcaa tttgactagc aatgacattg gacaagagct catcgtcgtt tttcaagacg
     3781 gcgacactgg tctcatcaca ggatgtctca aatgctaaaa tatatctatc cttcatctat
     3841 ttctctcttc atgataatgg cgtcctcgac tgggtcatgg tagtaggcct ttcgctcagc
     3901 gataactgcc atcttttctt tcttgtaaaa tgcttgcgct cgttgatttg actgtctgac
     3961 ttcgaggaaa atttccttgt ctgtcggcaa ttgagcaaac aaggctgacg caatgccctt
     4021 accctgatag gctcctttga cagcgatttg caggacttct gcttcaaaga gagtctcctg
     4081 aacagctaga aagccaatca cttctgcccc atcataagcc agagcatacc aagtctggtc
     4141 ttggaacagg tcagcttgga tttgttccag cgtccaagga ctgactgggt aaacagctat
     4201 cataacagcg tagatggctt tagctagatc aggctgctgt tggattcgtt tgatttctat
     4261 cataggcgtt taatgtaaga ctcgccagac tcggtatggt tcttgagcca gttttcctca
     4321 gcctcgacac gcttgaggta atttggcaca aagtcgtgca aggagtctgc ttctttgtcc
     4381 caggcccaaa gagctagatt agctgcattt ggcaaggttt ctttgaaatt agtccttggc
     4441 aagtattctt gaatctgctc aacaaagggg ccaacttctc cgacaaaggt tacctgacta
     4501 gtacccttga ctttttctag cacctcttca aaagataggt gcgcttctgc catgacaggt
     4561 ttggcatttt cataaaagcc tgcatagaca ttgttgcgac gcgcatccat gataggaacc
     4621 accaagcctt cttgttggcg tggcaccaga gccaatagac tagacatccc aactaactca
     4681 atgttcaggg tatgagccaa ggtcttggca gttgctaccg caattcgcaa gcctgtatag
     4741 ctacccggcc cttcagctac cacgattcgg tccaaatcct tgggtgtcca atccaaactt
     4801 gccatcaaaa aatcgatggc aggcataagg gtaatactgt gatttttctt aatattaatc
     4861 gtcgtctcgg caagaacctg cttatcctct aaaataacga gagaaagagc cttgctggac
     4921 gtatcaaaag ctaatacttt cataacacat tcctatcttt ttgtctgctt actattatac
     4981 tacaaaagct ggcacatggg aattttcttt gcccccagac aagagtgccc tcacttaact
     5041 aaaaataatt taaaaaaatg ctcacttttc cttttctttt ccgaatataa aagtgaacaa
     5101 gaaaaaaaga ggaaagttca atgacaaatt ttgacattct tgacaatcaa tttttatcct
     5161 tatctgaaaa tgaattatca gatattgatg gcggtctcgc tcccttggtt atctttggag
     5221 tagcagtatc ttggaaggct attgcaggtg gaacagcact tataggttct ggtttggcag
     5281 ctggttattt tttaggagga gattaatatg atgaaaaatt tgaacaacta tcgtgaaatt
     5341 tctaataagg aattgcaaga aatcaagggt ggctttggtg taggtgttgg tatcgcttta
     5401 tttatggcag gttataccat tggaaaagac cttcgtaaaa agtttggtaa gtcatgctag
     5461 ataagaaaca catttttaga aggataaatt ttattgtctt catctcttac agtttgctca
     5521 gcattctcaa tgatttgaac attactacta tccctttacc attcgattta tctgtttgta
     5581 ttgttttatt tttatgcttc aactctattt ttgagcagaa caatgactcc cataaaaata
     5641 ataagctttg aaaattccat tgtcatgtca tgttagaaaa atgcaaagac cacctcatct
     5701 tgatagatgg ggtggaattt tcgtgtcgta aatctactat ctctacatcc ccaaacaaaa
     5761 aaccccagca taagcagggc aatccaaagt aaaatacaaa ccaaacgaca taggtcacga
     5821 ggaggagaaa aagcgagtag agagtcacaa aggtaatttt cctcaagaac ttggtttgtc
     5881 atcgttccag tttggcaaat agaagatttc ctccataaac gcaagcaaca aaaacgataa
     5941 aagctagcaa acgaactccg atagcaaaag caaataagtt atacataggg caacctcctt
     6001 gacttaaaat ctatatggaa ttatgacaag caataaattt cacttccgtt atcaacataa
     6061 tacattttct ttatttttgc aaacgcttac caaagaaatc gtcccctaac tttctcgttt
     6121 ccgtctttta ctaatttttc attttgtggt ataattgaaa taattgtaac gaatcaaggt
     6181 caatctagac acaaaatgga atgaaatcaa gcaaatctct gctaaaagtt tggaataagc
     6241 tgacctgtaa atagaaagga actatatgat ttacaaagtt ttttatcaag aaacaaaaga
     6301 acgtagccca cgtcgtgaaa caacacgcgc gctttaccta aacatcgatg ccagctcaga
     6361 acttgagggt cgtatcgctg ctcgccaact tgtcgaagaa aatcgcccag agtacaatat
     6421 cgaatatatc gaactcttgt ctgacaaatt gctcgattac gaaaaagaaa ctggcgcctt
     6481 cgaaattacg gagttctaat atggcctaca ctcttaaacc tgaagaagtc ggcgtttttg
     6541 ccatcggtgg tctaggagaa atcgggaaaa acacttacgg aattgaatac caagacgaga
     6601 ttatcatcgt cgatgctggg attaaattcc cagaagatga cttgcttggt atcgactatg
     6661 tcattcctga ctactcttac atcgtagaca atatcgaccg cgtcaaggct gttttaatca
     6721 cacacggaca cgaggaccac attggtggga ttccgttcct actcaagcaa gcaaatgtcc
     6781 ctatttatgc tggaccgctt gccttggctt tgatccgtgg gaaactcgaa gaacacggcc
     6841 tcttgcgcaa cgccaaactt tacgaaatca accacaacac cgagttgacc tttaaaaatc
     6901 tcaaggcaac tttctttaga acgactcact ctattccaga acctttggga attgtcatcc
     6961 atactcctca agggaaaatc atctgtacag gtgactttaa gttcgacttt actccagttg
     7021 gagaacctgc ggacttgcac cgtatggcag ccctaggtga agatggagtg ctttgcttgc
     7081 tctctgactc gaccaatgct gaggttccaa cctttacaaa ctctgaaaag gttgtcggcc
     7141 agtccatcat gaagattatc caaggtatcg agggacgtat catctttgca tccttcgcct
     7201 caaatatctt ccgtctccaa caagcaacgg aagctgctgt taagactggg cgcaagattg
     7261 cggtctttgg tcgctctatg gaaaaggcca ttgtcaacgg aatcgatctt ggctacatca
     7321 aagctcctaa gggaaccttt attgagccaa atgaaatcaa agactatcct gcaggtgaga
     7381 ttctgatcct ctgtacaggt agtcaaggtg aacctatggc agccctctct cgtatcgcca
     7441 acggaaccca ccgtcaggta caactccaac caggcgatac cgttatcttc tcttctagtc
     7501 ccatccctgg aaacactact agcgtcaaca agctgattaa catcatttct gaagctggtg
     7561 tcgaagttat ccacggtaaa gtgaacaata tccatacatc tggacacggt ggtcagcaag
     7621 agcaaaaact catgctccgc ttgattaagc caaaatactt catgcctgtc cacggtgaat
     7681 accgcatgca aaaagtccac gctggactag cagtggatac tggtgttgag aaggacaata
     7741 tctttatcat gagcaatggc gatgtgcttg cccttactgc tgactcagct cgtatcgcag
     7801 gtcatttcaa cgcccaagat atctatgtcg atggaaatcg tatcggtgaa attggcgcag
     7861 ctgtcctcaa agatcgtcgc gatctatctg aagacggtgt cgttctggca gtcgcaactg
     7921 ttgacttcaa atcgcagatg attctatctg gtccagacat cctcagccga ggctttgtct
     7981 acatgagaga gtctggcgac ttgattcgcc aaagccagcg tatcctcttc aatgccattc
     8041 gtatcgcact gaaaaataag gatgctagcg tgcaatctgt caatggtgcc attgtcaacg
     8101 ctattcgccc cttcctctat gaaaataccg aacgtgaacc gatcatcatc ccgatgatcc
     8161 tcacaccaga tgaagaataa tactcttcaa accacgtcag cttcgccttg ccgtatatat
     8221 gttactgact tcgtcagttc tatccacaac ctcaaagcaa tgttttgagc aacctacggc
     8281 tagcttccta gtttgctctt tgattttcat tgagtacaag tcaacaaaac agctccgtct
     8341 tcggagctgt ttttctctat gctttctttt gagattaaaa ctcaatgaaa atcagaccag
     8401 aaggctagga aaatgcataa cttgagttag ctcccatagt tcggggaact atgggaggct
     8461 ggagatgaat caaagccaag ctttgaactc attcgtaaga agccgacgac gtatcatttt
     8521 gatttttgaa gagttttaga aatactacga tttttacctt ccagatacac catcaaaata
     8581 gaaatatctg ctgggtttac tcccgaaata cggctggctt ggccgatggt ttctggattg
     8641 atgagtttga acttctgacg agcttccgtc gcaatagaat cgatgtcatc ccagtcaata
     8701 ttggctggaa tgcgtttttc ttccatacgt ttcatcttgg caacctgatc catggctttt
     8761 gaaatatagc cttcgtactt gatttctgtt tcaatcaatt cgataatctt gtcatccaag
     8821 tcttctgcag ctggtccgat gaaggccacc acatcttggt aagaaacttc tggacggcga
     8881 aggaattctt tggctgtcac cgcatctgtc aacggcttga agcccatttc ctcaacctta
     8941 gcattggttt ccttgactgg cttgagtttg atactgtcta ggcgtttcat ctcattatca
     9001 aattgatttt tcttgatttc aaaacgagcc cagcgttcat catccacaag gccaatctcg
     9061 cgtcccatct cagtcaagcg catatcagca ttgtcatgac gaagaatgag acggtattca
     9121 gcacgactgg tcaagagacg gtaaggttca atggttccct tggtcaccaa gtcgtcgatc
     9181 atcaccccga tataaccgtc acttcgtttt agaatcaact caggtttacc ttggattttc
     9241 agagccgcat tgatacccgc gataatccct tggccagcag cttcttcata acctgatgtt
     9301 ccatttgtct gaccagcagt gaagagacct gagattttct tggtttccaa agtcgcacgc
     9361 aactgatgag gcaagaccat atcatactca atagcataac ctgtccgcat catctctgca
     9421 ttttccaaac ctttgatgga atgaaccaag tcacgctgga catcctcagg cagactggtt
     9481 gaaagtcctt gaacatagac ttcctcagta ttgcgtcctt ctggctcaag gaagagttgg
     9541 tgacgttcct tgtccgcaaa gcgcacaatc ttgtcttcaa tcgacggaca gtaacgaggc
     9601 cccactccct tgaccacacc tgtaaacata ggcgcacggt ggaggttgtt ttggataatc
     9661 tcatgactgg taccattggt ataggtcaac cagcatggca cttgatcttt gacataatcc
     9721 tcatcacgtg aagtgtatga gaaatgatta ggcacttcgt ctcctggctg aatttccgtc
     9781 acatcgtaat tgatagaaga agccttgaca cgtggagggg ttcctgtctt gaaacgaccg
     9841 atttcgagac ccagttcctt gagattgtca gctaggttaa tagaagccaa gctgtggtta
     9901 ggacctgatg agtacttgag gtctccgatg ataatttccc cacggagagc agtccctgtc
     9961 gtcacaataa cagccttagc agcatattct tgatgggtgg ctgtacgcac accgacaacc
    10021 ttgccatctt ccaccaaaat ctcatcaatc atggtttgac gaagggtcag attttcttga
    10081 ttttcaactg tcttgcgcat ttccttagag taaagttcct tatcagcctg cgcacgaagg
    10141 gcacgaacgg ctgggccctt ccctgtgttg agcatcttca tctggatgta agtcttgtca
    10201 atggtcttgg ccatctcgcc accgagggca tcgacttcac gtacgacaat ccccttagca
    10261 gaaccaccga tagagggatt acaaggcatg aaagccagca tttcaatatt gatggtcgca
    10321 agcaggacct tacagcccat acggctagcg gccaaggaag cctcaacccc agcgtgtccc
    10381 gcaccaatta caataatatc gtattcttca gtaaaatgat aagtcatgtt tctctcctat
    10441 tcctcaagat gaatgtgtct tagttggcct tcccaatctg gtagggccgt ttttaaaaag
    10501 gctggaacta gctggatatc ctcaagctta tccaagtcaa tccactcaca gggctgcctt
    10561 ttctcatctt cctgcatggt caatggggca tcctcaagta agtccaccag ataatgaaac
    10621 tcgatattgt gataggaaac accgtcctgt tcaaaacgat tttcaaccac aaaagctagc
    10681 tgcccagctt gagctttgac acccagttct tccttcactt cacggactac cgcgtcttcc
    10741 gtgctttcat tgacttgaat cgcaccgccg atagtgtaat acttgccctt gtctttggtg
    10801 actagaagct tgtgattttg aagaatcaaa gctgtcgccc gaacaccaaa aaccgtattg
    10861 tctacttttg tccgaaagtc ttgttgagtc attcttgtcc tttcccttaa acgacacaaa
    10921 aacagtcaaa actacaaaga agtgcaggac aaaaaagcct gcaacatcca tgagctttga
    10981 ccatcatttc tattgctgtt attattgtag caaattgaga aaatttgtca atctaaatgt
    11041 actgacaaac ttgtccatcg cggtaagcat tgtcaatcaa accgccacct agacactctt
    11101 cgccatcgta aaagacaact gcctgtcctg gtgtaatcgc gcgttgtggt tccgcaaaga
    11161 taacttctgc cttgtctcct ttgacatgga cggtcacctt agagtcaggc tgacggtaac
    11221 ggaatttagc cgtacattct agcgtaaact cttctggcat ttcacgagta aagtggactt
    11281 gactggcttc taggctagtt gacatgagcg aatcatggta gaaaccttgg cctacataga
    11341 gaatattctt gcttagatct tttccgacaa cgaaccaagg ggcattgtca ccgccgtgtt
    11401 gcccaccgat accgagtccg ccacgctgac caatcgtata gtacatcaga ccagcatgct
    11461 cgcccatatc gcgaccatcc acagtcatca tacgaccagg ctgagctggc aggtagttgc
    11521 tgagaaagtt tttaaagttc ttttctccga taaagcaaat ccctgtcgag tctttcttct
    11581 tagcagtcga aagacctgct tcttctgcta gttttcgaac ttcaggcttt tccaaatgtc
    11641 ctagtgggaa catggttttt tgaagttgtt cttgcgaaag ttggctgagg aaataggtct
    11701 ggtccttgcc attgtccacg ccacgaagca tgtgaacggt accatcctca tcacgcgcca
    11761 ctcgagcata atgcccagtc gctacatagt ctgcccccaa ggttatggca tagtccaaaa
    11821 aggccttgaa cttgatttcc ttgttgcaca taacgtccgg atttggcgtg cgccctgcac
    11881 ggtattccgc taggaaatac tcaaaaacgc ggtcccagta ctctttttca aaattgacag
    11941 agtagtaggg aatgccaatc tggtctgcca ccgcaaccac atccttgtaa tcttcggtcg
    12001 ccgtacagac gccgttttca tctgtgtcat cccagttctt catgaagata ccgatcacat
    12061 cgtaaccctg ctccttgagc aaaagagccg tcaccgacga atcaacacca ccactcatcc
    12121 ccacgacaac acgtgtttta gagttatcac tcatggtaag tctcccatct attcgtttat
    12181 tcacgattga aggtcgtgtg tgcttcacga ttgaaggtcg cttgagctgt attcattata
    12241 acacgcttgg ttagagaaga caaggaagag cttaaaaatt gttttttaaa acttatagtc
    12301 aatatacaga ttatgcgaat gatgtgagta taccttcttc ttatcttcat tcaaataact
    12361 ataataaata aaacaaccca ttttctctaa tctatttttc
//
LOCUS       DAVKAO010000047        11978 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKAO010000047 DAVKAO010000000
VERSION     DAVKAO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.047"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            672..1349
                     /locus_tag="VWX16_001458"
     CDS             672..1349
                     /locus_tag="VWX16_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9158788.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQVKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVETGEM
                     ELSARDTRFGPFLSREISPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            1336..2133
                     /locus_tag="VWX16_001459"
     CDS             1336..2133
                     /locus_tag="VWX16_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9158789.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            2150..3253
                     /locus_tag="VWX16_001460"
     CDS             2150..3253
                     /locus_tag="VWX16_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9158790.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            3260..3406
                     /locus_tag="VWX16_001461"
     CDS             3260..3406
                     /locus_tag="VWX16_001461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158791.1"
                     /translation="MKIKEQTRKLAAGYSKRCFEVVDRTDEVSNHTHGKATLTWFELD
                     FRRV"
     gene            3509..4528
                     /gene="galE"
                     /locus_tag="VWX16_001462"
     CDS             3509..4528
                     /gene="galE"
                     /locus_tag="VWX16_001462"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9158792.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEELTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPYTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            4530..5483
                     /locus_tag="VWX16_001463"
     CDS             4530..5483
                     /locus_tag="VWX16_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9158793.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(5545..5751)
                     /locus_tag="VWX16_001464"
     CDS             complement(5545..5751)
                     /locus_tag="VWX16_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9158794.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            5803..6279
                     /locus_tag="VWX16_001465"
     CDS             5803..6279
                     /locus_tag="VWX16_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9158795.1"
                     /translation="MAKEPWQEDIYDQEESRAERRPRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EGAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            6288..6959
                     /locus_tag="VWX16_001466"
     CDS             6288..6959
                     /locus_tag="VWX16_001466"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9158796.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHSISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVRKKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            7090..7428
                     /locus_tag="VWX16_001467"
     CDS             7090..7428
                     /locus_tag="VWX16_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9158797.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            7431..8060
                     /locus_tag="VWX16_001468"
     CDS             7431..8060
                     /locus_tag="VWX16_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9158798.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            8300..9451
                     /locus_tag="VWX16_001469"
     CDS             8300..9451
                     /locus_tag="VWX16_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9158799.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            9769..10518
                     /gene="truA"
                     /locus_tag="VWX16_001470"
     CDS             9769..10518
                     /gene="truA"
                     /locus_tag="VWX16_001470"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9158800.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQVI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            10508..11281
                     /locus_tag="VWX16_001471"
     CDS             10508..11281
                     /locus_tag="VWX16_001471"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9158801.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNDLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVSVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            11259..11720
                     /locus_tag="VWX16_001472"
     CDS             11259..11720
                     /locus_tag="VWX16_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9158802.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLLDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMIGMIVGFILSQSIKKIK"
BASE COUNT         3414 a         2248 c         2718 g         3598 t
ORIGIN      
        1 gagcttatgt gtattttcca cccttgtgca ggcaccttat ctgataaata atagtaattc
       61 caaatatcac cctgttggaa atttttatca cattttaaac attctttctc taataaatcc
      121 ataacaaact cctaattatt aaaataggct agagacacag cactagccta ttccgtaagt
      181 aaagctactt attttatata aaatataact agcgacctgt aattaaacaa gtaaaagaac
      241 aatacttagg gagacctgtg acaatgacat cttcctctgg aagtagttct aattcaaaca
      301 atgtttgttc catgactgta tcttcctgtt atgataaatt ccagaaagca tagagcaaat
      361 tcgtggttcg tctgactaaa tttcttatct gaacaccacc ttcaaataca aattttagtc
      421 aaacttttat tttttctaac tttctaatta tattctacca tgtgtttttt atatgtcaag
      481 aattttctga ataaactgtt tattttttat tttttgatat ttttgcaaat tgtttaaaat
      541 ctttcaattg gattcactta ttactttagg acattgaaac ttctttcgcc agaggtgctt
      601 atattactgg gaagttgaag attgaaatgt ggtataataa gaggtaatag agttttggaa
      661 agtgagagaa gatgatttca aagagattag aattggtagc ttcctttgtg tcacaggggg
      721 ctattttact agatgtggga agtgaccatg cttatctgcc tatcgagttg gttgagagag
      781 gccaagtcaa aagcgctatt gcaggtgagg tggtggaagg tccctatcag tctgcggtta
      841 aaaatgttga ggctcacggc ctaaaggaga aaatccaagt ccgtttagcc aatggcttgg
      901 cagcttttga agagactgac caagtgtctg tcattaccat tgctggcatg ggtggtcgtt
      961 tgattgctag gattttagaa gaaggtttgg gtaagttagc taatgtagag cgtttgatcc
     1021 tccagcccaa taatcgtgaa gacgacttgc gtatctggct acaggatcat ggattccaga
     1081 ttgtagcaga aagcatctta gaagaagctg gaaagtttta tgagattcta gtagtggaaa
     1141 ctggagaaat ggaattatca gcaagagata cccggtttgg ccccttcttg tcaagggaaa
     1201 tcagcccagt atttgtccaa aaatggcaaa aagaagctga gaagctagag tttgccctcg
     1261 gacaaatccc agaaaaaaat ctggaagaac gtcaagttct agtagataag attcaagcta
     1321 tcaaggaggt gctccatgtt agcaagtgaa gtgatccaag cttatgaagc cttttgccct
     1381 caggaatttt ctatggaggg agacagccgt ggtctgcaaa ttggcacttt agacaagggt
     1441 atccaaaggg tcatggttgc cctcgatatt cgtgaagaga cagtggcaga ggccattgaa
     1501 aagggtgtgg acttgattat cgtcaagcac gcgcctatct ttcgtcctat caaggacttg
     1561 cttgccagcc gtccgcaaaa tcagatttac atcgacctga ttaagcatga catcgcagtt
     1621 tatgtcagcc ataccaatat tgatatcgtt gaaaatgggc tcaatgactg gttttgtcag
     1681 atgctaggaa tcgaggagac gacttatctg caggaaacag gtccagaacg tggaattgga
     1741 cgtattggga atattcagcc tcagacattt tgggaattgg cccaacaggt caagcaagtc
     1801 tttgacctag atagccttcg aatggtgcat tatcaagaga atgatttgca gaagcctatt
     1861 tcaagagtag caatttgtgg tggaagcggg cagtctttct ataaggatgc tttggcaaag
     1921 ggggcagatg tctatatcac tggtgatatc tactaccata ctgctcagga tatgctgtct
     1981 gatggcttgt tagcattgga tccaggtcac tatatcgaag tgctttttgt ggaaaaaatc
     2041 gcagcactcc ttactcaatg gaaagagaaa aaaggctggg aactagagat tttacctagt
     2101 caagcatcga ccaatccttt ccgtcatatc tagttagaaa gtgaagacaa tgaaaaaagt
     2161 tgccattatt ggagcaggaa ttgtgggagc aacagctgcc tactacctct cgagagaaag
     2221 tgacctagag gtgaccgttt ttgaccatga gcaaggtcaa gccaccaagg ctgcagcagg
     2281 aattatcagt ccttggtttt ccaaacgccg taataaagcc tggtacaaga tggcgcgctt
     2341 gggggctgat ttttatgtgg atttattagc tgatttagag aaatcaggac aaaaaatcga
     2401 cttttaccag cgttcgggag tctttctctt gaaaaaggat gaatctaagt tggaagaact
     2461 ttatcaactg gccctccagc gcagagaaga atctcccttg atagggcaat tagccattct
     2521 ggaccaagcc tcagctaatg aattattccc tggtttgcag ggatttgacc gcctgctcta
     2581 tgcttctggt ggagcgagag tagatggcca cctcttagtg actcgtttgc tggaagccag
     2641 tcaggtcaag ctggtcaaag aaaaagtgac tctgacaccg ttagcatcag gctaccagat
     2701 tggtgaagag gagtttgagc aggttatttt ggcgacggga gcttggttgg gggacatgtt
     2761 agaacccttg ggttatgaag tagatgttcg tcctcaaaaa ggacaactac gagattatca
     2821 gctggtccaa gacatggaag cttaccctgt tgtcatgcca gaaggggagt gggatttgat
     2881 tccttttgca ggtgggaaat tgtccttagg cgctacccac gaaaatgaca tgggatttga
     2941 tttgacggta gatgaaacct tgctccaaca aatggaggag gccaccttga ctcactatct
     3001 gattttggct gaagctactt caaaatctga gcgtgttgga atccgtgcct acaccagtga
     3061 tttctctcct ttctttgggc aggtgcctga cttaactggt gtctatgcag ccagtggact
     3121 aggttcatca ggcctcacaa ctggtcctat tattggttac catttagctc aactgatcca
     3181 agacaaggag ttgaccttgg accctgaaaa ctacccaatt gaaaactatg tcaaacgagt
     3241 aaaaagcgaa taatactcaa tgaaaatcaa agaacaaact aggaaactag ccgcgggtta
     3301 ctcaaagcgc tgctttgagg ttgtggatag aactgacgaa gtcagtaacc atacccatgg
     3361 caaggcgacg ttgacgtggt ttgaattgga ttttcgaaga gtataagcat tttactgaaa
     3421 ttttatcagg tagtttagga tggcaaatga cattccctgt caaaaatggt aaaataagaa
     3481 aaaataatct gagaatcgag gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc
     3541 cggttttatt ggaacccaca ctgttattga attgatccaa gcaggccatc aagttgttgt
     3601 ggtggataat cttgtcaata gcaatcgtaa gagtttagaa gttgttgaaa gaatcacagg
     3661 agttgagatt cctttctatg aggcagatat tcgtgacaca gataccctca gagatatttt
     3721 caagcaggaa gaactgactg gtgtcattca ctttgctggt ttgaaggctg ttggcgaatc
     3781 aacacgtatc cctcttgcct actatgacaa caatatcgct ggaactgtca gccttttgaa
     3841 agccatggaa gaaaacaact gtaaaaacat catcttcagt tcttctgcga cagtttacgg
     3901 ggatccgtac acagtgccca tcttggaaga tttcccactt tcagtgacca acccatacgg
     3961 tcgtaccaag ctcatgctag aggaaatttt gaccgatatt tacaaagcag actcagaatg
     4021 gaatgttgtc ttgcttcgtt actttaaccc aatcggagcc catgagagtg gtgatttggg
     4081 agaaaatcca aacggtattc caaacaatct cttgccatat gtgactcaag tagccgttgg
     4141 aaaattagag caagtgcaag tgtttggaga cgattacgat acggaagatg gaacaggtgt
     4201 tcgtgactat atccacgttg tcgatttggc taagggtcac gttgcagctt tgaaaaaaat
     4261 ccaaaaaggt tcaggactaa acgtttataa ccttggaact ggtaaaggtt actcagttct
     4321 tgaaattatc caaaacatgg aaaaagcggt gggacgtcct attccttacc gcatcgtaga
     4381 acgtcgccca ggtgatatcg ctgcctgcta ctcagacccg gcaaaagcta aagcagaact
     4441 cggttgggaa gcagaactcg acatcaccca aatgtgtgaa gacgcatggc gttggcaaag
     4501 caagcatcca aatggatttg aagactaaga tgatgatttc aatcatcgtc ccttgtttaa
     4561 atgaagagga agtacttcct cttttttatc aggctctgga agctttactt ccagatttgg
     4621 aaacagaaat cgagtatgtc tttgtcgatg atggatcaag tgatgggacc ttggaactct
     4681 taaaggccta tcgggagcaa aatccggcag tccattatat ttctttctct cgaaattttg
     4741 gcaaagaagc agccctttat gcaggcttgc aatatgcgac aggagatttg gtggtggtga
     4801 tggatgcaga cctccaagat cctcctagta tgttgtttga gatgaaaaat gtactagaca
     4861 aaaatgtaga cttggactgc gttgggacac ggagaactag tcgggaggga gaacccttct
     4921 ttcgcagttt ctgtgctgtt ctcttttatc gcctcatgca aaaaatcagc ccagtagctc
     4981 tgccgtcggg tgtccgtgac tttcgtatga tgagaaggtc tgtggtcgat gccattttaa
     5041 gcttgactga gtccaatcgt ttttctaagg gactctttgc ctgggtcggc tttaaaaccc
     5101 actatctgga ctatccaaat gtcgaaaggc aggctggcaa gaccagttgg agttttaggc
     5161 aactcttttt ttactccatt gaagggattg ttaatttttc agatttccct ttgactatag
     5221 cctttgtagc tggtctccta tcttgttttc tttctctgct gatgaccttt tttgttgtgg
     5281 ttcggaccct cattttgggc aatccgacat ctggttggac ctctctgatg gctgttattc
     5341 tctttcttgg aggcattcaa ctcttgacca ttgggattct cggcaagtat atcagtaaga
     5401 tttatttaga gactaaaaaa agaccacttt atcttatcaa agaaaaaagt gaccttcctg
     5461 attttacaga aaaaataaag tgaaaagact ataattttac atggaaatgt gctaaactag
     5521 agggagtgga ttatcgccat tgatttaggc ttcctggttt ccaatcaggg cgcgagttgg
     5581 gcaattttta acagcctcta agacatcctg actaggagaa atttcttttt ccagttggtc
     5641 agggtcatcg taaaaacgca cgattccatt atcatggtaa tcaaataaat cagaataagt
     5701 ttggcaaagc ccacaggcga tacatcgttc aggtataagt gtgattttca tgttcatatt
     5761 tatattgtaa taagaaaagt aaaaaaaaac aaggagtaag gtatggcaaa agaaccgtgg
     5821 caagaagata tctatgatca agaagaatca agagcagagc gtcggcctcg aaaccacgga
     5881 ggggctgata ggatggctaa tcgtattttg acgatcctag ctagtatttt ctttgtaatt
     5941 gtggtggtga tggtcatcgt tctcatctat ctatcatcgg gggggagtaa tcgcacagca
     6001 gccttaaaag actttcatga ttctgatgca agtgtagtac aaatctcatc ttcaagcagt
     6061 tctcagcctg agcagagttc agagccagaa tctacttcta gtagttcaga aggagctgct
     6121 aatcctgaag gaactattaa agttctcgca ggagaagggg aagcagctat tgccgctcgt
     6181 gcaggaatct ccattgctca gttagaggcc ttgaatcctg ggcacatggc tacaggatct
     6241 tggtttgcta atccaggtga tgttataaaa ataaaatagg agccgatatg aaaacaattc
     6301 aaattgctat tgatggtcct gcttccagcg gtaagagtac ggtcgcaaag attattgcta
     6361 aggattttgg attcacctac cttgatacag gagctatgta tcgtgcagcg acctatatgg
     6421 ctcttaagaa ccaattagga gttgaagaag tcgaagccct tctagccttg ttggaccagc
     6481 attcaatcag ctttggacgt tcagaaactg gagaccagct tgtttttgta ggagatgtgg
     6541 atattaccca tcctatccgt gaaaatgaag tgaccaatca tgtttctgct attgcagcaa
     6601 ttcctgaagt gcgtaagaaa ttggtttctc tccaacaaga aattgcccag caaggcggga
     6661 ttgtcatgga tggtcgcgat attggaactg ttgtattgcc acaagcagaa ttgaaaattt
     6721 tcctagtagc ttctgttgat gagagagcag agcgtcgtta caaggaaaat attgccaagg
     6781 gaattgaaac agaccttgaa accctaaaaa aggaaattgc tgcgcgtgac tacaaggata
     6841 gtcatcgtga gacttctcct ctcaaacaag cagaggatgc tgtctacctt gatacaactg
     6901 gtttgaacat tcaagaagta gttgaaaaaa tcaaagcaga agctgaaaaa agaatgtaaa
     6961 tgtagaaaag ccgatagatt gatatcggtt tttttctaaa tttgtcaaaa tgctgatttt
     7021 agggtataat agttgctgtt cgagaaattt tgattttcaa agaatagtga atgatgataa
     7081 ggagaaatca tgaacaattt accaaattgt ccaaaatgta actcagagta tgtctacgaa
     7141 gacggtgccc tactggtttg cccagagtgt gctcatgagt ggaatcctgc tgaagttgca
     7201 gaagtagaag aaggtcttgt cgctatcgat gccaacggaa ataaattggc tgatggtgat
     7261 acagtaactc tcatcaagga cttgaaagta aaaggtgcgc caaaagattt gaaacaaggg
     7321 acgcgcgtga aaaatatccg catcgtagaa ggcgaccaca atatcgactg taagattgat
     7381 ggttttggtg ccatgaaact caaatcagag tttgtgagga agatttaagc atgtcaaaac
     7441 actataaact tgtattttat agccgtatct tcttgtttct agcggctttt acgggagttt
     7501 atcttgaaat tactaagcat ggtggttttg ggatgcttct ctattacacg gttctgtcca
     7561 acctcttggt aactattttt accctttatc ttctaaaggt tatgagccgt gtaggtgaaa
     7621 attggcaaag gccaagtctc ttgcgcttaa aaggtggggt caccatgagt atcatgatta
     7681 cctgtgtgat ttaccatttc ctcttggcgc ccattgcgac taatttctat accctagaaa
     7741 atttcctttg ccactatatc gttcccatct ggtttttagc ggataccctc ttttttgaca
     7801 aacagggtca atacaagatt tgggatccag cagtgtggac cattttaccc tttctgtata
     7861 tgatgtttgc tctttttaat ggcttggttc taaaactcaa tattccaaat gccaaggata
     7921 atcctttccc ttacttcttt ttgaatgtga acaagggttg gaatgttgtg tttaagtggt
     7981 gtctgattat ctttgttgcc tatatggtag caggatttat tttctacttt atcaagcaaa
     8041 tcaagagaaa gtcatcctaa tactcttcga aaatctcttc aaaccacgtc agtttccatc
     8101 tgcaacctca aaacactgtt ttgaacaacc tgcggctagc ttcctagttt gctctttgat
     8161 tttcattgag tataagatac aagggattca ttttcgaatt taagaaggag tctacaaacc
     8221 aatgaggctt cttcttttct tgcttatttg ataactatca aaaaagtaga agattaagag
     8281 aaatatagaa agagatttca tgaaacaatt tttagaacgg gccagcattt tggctctctc
     8341 cctcgttttg attacctcct tttccatttc gagtgcccta ccagccatgt ttgactatta
     8401 tcagggttat tctaaggaac aaattgagct cttggtgagc ttgccttcct ttggaatcat
     8461 gatgatgtta ctgctaaatg gtttcttaga aaaaatattt cctgagcgct tacagattag
     8521 tttgggcttg ctgattttat cattgagcgg tacagctccc ttctggtacc aagcctatcc
     8581 ctttgtcttt ggaacacggc ttctctttgg tttgggtctt gggatgatca atgccaaggc
     8641 catttctatt atcagtgaac gctaccaagg aaaaaggcga attcagatgt tagggctacg
     8701 cgcttctgca gaggtcgttg gagcttctct cattaccttg gccgtcggtc agttgttggc
     8761 ctttggttgg acagctatct ttctagccta tagtgctgga tttttggtgc tgccccttta
     8821 tctgctcttt gtcccttatg gaaaatcaaa gaaagaagtc aagaaaagag cgaaggaagc
     8881 aagtcgttta actcgagaaa tgaaaggctt gatttttacc ttagctatcg aagcggcagt
     8941 tgtagtttgt accaatacag ctattaccat ccgtattcca agtttgatgg tggaaagagg
     9001 attgggggat gcccagttat ctagttttgt tcttagtatc atgcagttga tcgggattgt
     9061 ggctggggtg agtttttctt tcttgatttc tatctttaaa gagaaactgc tcctctggtc
     9121 tggtattacc tttggcttgg ggcaaatcgt gattgccttg tcttcatcct tgtgggtggt
     9181 agtagcagga agtgttctgg ctggatttgc ctatagtgta gtcttgacga cggtctttca
     9241 acttgtctct gaacgaattc cagctaaact cctcaatcaa gcaacttcat ttgctgtatt
     9301 aggctgtagt ttcggagcct ttacgacccc attcgttcta ggtgcaattg gcttactaac
     9361 tcacaatggg atgttggtct ttagtatctt aggaggttgg ttgattgtaa tctctatctt
     9421 tgtcatgtac ctacttcaga agagagctta ggattgattc ctaagttttt cttttgatac
     9481 tcaatgaaaa tcaaagagca aactatagtt gattgagttt ggaatagtat gctgtagctg
     9541 ctaaaatatt tctagaaatt aatttgactt tcctaataga gttgttcata tcttatttca
     9601 atctactata gaaagctagc cgcaggttgc tcaaaatagt gtgttgaggt tgcagatgga
     9661 aactgacgtg gtttgaagag attttcgaag agtatgagat ttttataccc atacagtatt
     9721 tgttttaggt ccttgtttac agactaattt cttgataaaa tagaggctat gacgagatat
     9781 aaagcaacta tttcctatga tggttatgcc tttgctggct ttcagcgcca gcctcatgcg
     9841 cgtagcgttc aggaagaaat tgaaaaaacc ttgaccagat taaataaagg gcaagtcatt
     9901 actgttcacg gtgctggtag gacagatagt ggggttcatg ccctgggaca ggtcattcat
     9961 tttgacctgc cttatcagat ggatgaggag aaactccgtt ttgccttgga tacccagtct
    10021 cctgaagata ttgatgtgat ttcgattgag cttgtggcag atgattttca ttgccgttat
    10081 gccaagcata gcaagaccta tgagtttact gtggatagag gacgtcccaa aaatccgatg
    10141 cgccgtcact atgctaccca ctttccctac ccactcgatg tggaacgaat gcagattgca
    10201 atcaaaaagc tagagggaac ccatgatttt accggtttta cagcctctgg gactagtgta
    10261 gaggataagg ttcgcaccat cacagaagct agtttaatag tcgatgagac aggacaattt
    10321 ttgaccttta ccttttcagg aaatggtttc ttgtataaac agattcgcaa tatggtgggg
    10381 actcttctca aaatcggtaa caaccgcatg ccagtagagc agattgatct catcttggag
    10441 aagaaggaca ggcaacttgc aggtcccact gcagcaccaa atggtttgta tttaaaggag
    10501 attcgttatg aagaataatc gtattttagc actttctgga aatgatattt ttagtggtgg
    10561 tggactgtcg gctgatttgg ctacctatac cttgaacgac ttgcatgggt ttgtagcagt
    10621 gacttgtttg acagccttga cagaaaaagg atttgaagtc tttccaactg atgataccat
    10681 ttttcaacat gaattagata gcttgcgtga tgtggaattt gggggaatta agattggtct
    10741 tctccctact gtcagtgtgg ctgagaaggc cttggacttt atcaaacaac gcccaggagt
    10801 atctgtggtg ctggatcctg tcttggtctg caaggaaacg catgatgtag ctgtcagtga
    10861 gctctgccaa gagttgattc gctttttccc ttatgtcagt gtgattacgc ctaatctccc
    10921 agaagcagaa ttattatccg gtcaggaaat taaaaccttg gaagacatga aaactgcagc
    10981 gcagaaattg catgatttag gagcgccagc agtcattatc aagggaggca atcgtcttag
    11041 tcaggacaag gctgtggatg tcttttatga tggacagacc tttactatcc tagaaaatcc
    11101 agttatccaa ggccaaaatg ctggtgcagg ttgtaccttt gcctctagca ttgccagtca
    11161 cttggttaaa ggtgataaac ttttgccagc agtagaaagc tctaaggctt tcgtttatcg
    11221 tgctattgca caagcagatc agtatggagt aagacaatat gaagcaaaca aaaacaacta
    11281 aaatcgccct tgtatcccta ttaaccgccc tttctgtggt tctaggttat ttcttaaaaa
    11341 tcccaacacc tacaggaatt ctaactcttt tagatgctgg tgtcttcttt gcggcctttt
    11401 actttggtag tcgtgaagga gcggtagtcg gaggactagc aagttttttg cttgacctct
    11461 tatcaggcta ccctcagtgg atgtttttta gcttggtcaa ccatggcttg cagggatttt
    11521 tcgcaggatt taaaggaaaa agtcagtggt taggccttat tttagcaact attgccatgg
    11581 tagggggcta cgccttgggt tctactttga tgaatggctg ggcagcagcc ctcccagaaa
    11641 ttctaccaaa ttttatgcaa aatatgatag ggatgattgt aggatttatt cttagtcaaa
    11701 gtatcaagaa gattaagtaa tactcttcga aaatctcttc aaaccacgtc agcttccatc
    11761 tgcaacctca aaacactgtt ttgagcagcc tgcggctagc tttctagttt gcactttgat
    11821 tttcattgag tataaaaaga tcttgggaaa agactcaatt tcagtagaaa aggaagtaaa
    11881 tcttcccaca ttaaaacgca tattaccaag gttcttatga actgcacccc aaaagttaga
    11941 caaaaaaaat ctaacttttg ggtgttttta ttatgaaa
//
LOCUS       DAVKAO010000048        11817 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKAO010000048 DAVKAO010000000
VERSION     DAVKAO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11817
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.048"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..189
                     /locus_tag="VWX16_001473"
     CDS             <1..189
                     /locus_tag="VWX16_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9158803.1"
                     /translation="NEELELIQGGADPYGKNPNGRYDWEIEPVLTLPVHGFCPRGTYD
                     LGYIGGGNHLCKGSAARF"
     gene            243..914
                     /locus_tag="VWX16_001474"
     CDS             243..914
                     /locus_tag="VWX16_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9158804.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALAPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(966..1163)
                     /locus_tag="VWX16_001475"
                     /pseudo
     CDS             complement(966..1163)
                     /locus_tag="VWX16_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1156..2064
                     /locus_tag="VWX16_001476"
     CDS             1156..2064
                     /locus_tag="VWX16_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9158805.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2061..2522
                     /locus_tag="VWX16_001477"
     CDS             2061..2522
                     /locus_tag="VWX16_001477"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9158806.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2512..3399
                     /locus_tag="VWX16_001478"
     CDS             2512..3399
                     /locus_tag="VWX16_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9158807.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3402..5297
                     /gene="cbpE"
                     /locus_tag="VWX16_001479"
                     /gene_synonym="pce"
     CDS             3402..5297
                     /gene="cbpE"
                     /locus_tag="VWX16_001479"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9158808.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIELYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHLDTKNSNTKNFIKNLSPEIIVQ
                     TSSSPIGSYDSGQEINREYISWLESMGIQHINAASKDYDATVFDIRQDGLVNISTSYK
                     PIPSFQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNN
                     RWFYLTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQY
                     KGQWYYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWL
                     KKANDWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVP
                     IEKSATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSV
                     NKSESEQ"
     gene            5377..6507
                     /locus_tag="VWX16_001480"
     CDS             5377..6507
                     /locus_tag="VWX16_001480"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9158809.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     HVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6517..7779
                     /locus_tag="VWX16_001481"
     CDS             6517..7779
                     /locus_tag="VWX16_001481"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9158810.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQVLLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADDDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7783..8580
                     /gene="proC"
                     /locus_tag="VWX16_001482"
     CDS             7783..8580
                     /gene="proC"
                     /locus_tag="VWX16_001482"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9158811.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8800..9438
                     /locus_tag="VWX16_001483"
     CDS             8800..9438
                     /locus_tag="VWX16_001483"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9158812.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9435..10325
                     /locus_tag="VWX16_001484"
     CDS             9435..10325
                     /locus_tag="VWX16_001484"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9158813.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10365..10682
                     /gene="yabA"
                     /locus_tag="VWX16_001485"
     CDS             10365..10682
                     /gene="yabA"
                     /locus_tag="VWX16_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9158814.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10685..11554
                     /gene="rsmI"
                     /locus_tag="VWX16_001486"
     CDS             10685..11554
                     /gene="rsmI"
                     /locus_tag="VWX16_001486"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9158815.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKTPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASKGVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
BASE COUNT         3515 a         2045 c         2805 g         3452 t
ORIGIN      
        1 aatgaagaat tagagctgat acaaggtgga gcagatccat atggtaaaaa tcctaatggt
       61 aggtacgatt gggaaataga accagtatta actctgccgg ttcatggatt ttgtcccaga
      121 ggcacctatg atttaggata tattggagga ggcaatcatc tttgcaaagg aagtgctgcg
      181 agattttaag taaaatttat tagtaatatg aagaaacaag tggagaaagc agagaattta
      241 atatgaaaaa acgggctatt caaattttac tagcattgtc cttaattttt tacaaatcaa
      301 cttggttttg gagagttttc aatcacctcg caaagcccta tctaccagca agtcgtgaat
      361 tttttcagat tctgcttttg atggagagcg gagttctttt cttagcggtc atctatctac
      421 tggtttttgc aggaaagaaa atttttcatt tcaagtggca gctgaggtac ttcatctacc
      481 ttttactggg ctacatcatt tcatatatgt ctgacttcct cttttcgtat ttcatatccc
      541 tgtcttcaaa tcagatttct ttgaatgaaa cggtagaaat gatggggaga caggagttcc
      601 cttatttctt gctcatcgtt tgcttcatcg cccctattgc tgaggaattg atttatcgag
      661 gtgtgcttat gacaaccttt ttcaaaaact caccttggta cggagatgtt ttgctttctg
      721 ctattatttt cggttatatt catatcaatt ttgctttagc tcctcttgct tttttcattt
      781 atgctagtgg aggtcttatt ttagctctat tgtatcgcat gactaaaaat ctctactatc
      841 caatactagt tcatattctc attaatatca ctgccttctg ggatgtgtgg ttgctcctat
      901 tttcaggaag ttagcttact aaaataatgt cggaactttc cggcattttc ttttttcaca
      961 aatagtcaac gtttttcttt tcgatattgt agtggtgtgt atccagttat ttttttgaat
     1021 tgattttgaa aataaggttg gcttgagaaa ggcagatagt gaagatagtt aagaagaata
     1081 ggatgttctt ttttcctttt tggaaaactt ctaaaatatg gtataatgaa aagataaaga
     1141 agttaggggt agaagatgaa cattcaacaa ttacgctatg ttgtggccat tgccaatagt
     1201 ggtactttcc gtgaagctgc tgaaaagatg tatgttagtc agccgagtct gtctatttct
     1261 gttcgtgatt tggaaaaaga gttgggtttt aagattttcc gtcggaccag ctcagggact
     1321 ttcttgaccc gtcgtgggat ggaattttat gaaaaagcgc aagaattggt taaaggattt
     1381 gatatttttc aaaatcagta tgccaatcct gaagaagaaa aagatgaatt ttctgttgct
     1441 agccagcact atgacttctt gccaccaact attacggcct tttcagagcg ctatcctgac
     1501 tataagaact tccgtatttt tgaatcaact actgttcaaa tattagatga agtggcgcaa
     1561 gggcatagtg agattgggat tatctacctc aacaatcaaa ataaaaaggg gattatgcaa
     1621 cgggttgaaa aattaggtct ggaggtcatc gaattgattc ctttccatac ccatatttat
     1681 ctccgtgagg gtcatccttt agcccagaaa gaggaattag tcatggagga tttagcggat
     1741 ttaccaacgg ttcgtttcac tcaagagaaa gacgagtacc tttactattc agagaacttt
     1801 gtcgatacca gcgctagctc acagatgttt aatgtgacag accgtgccac cttgaatggt
     1861 attttggagc ggacggacgc ctatgcgaca ggttctggat ttttagatag tgacagtgtt
     1921 aatggcatta cagttattcg tctcaaggat aacctagata accgcatggt ctatgttaaa
     1981 cgtgaagaag tggagcttag tcaagctggg actctcttcg tagaagtcat gcaagaatat
     2041 tttgatcaaa agaggaaatc atgaaaaaaa gagcaatagt ggcagtcatt gtactgcttt
     2101 taattgggct ggatcagttg gtcaaatcct atatcgtcca gcagattcca ctgggtgaag
     2161 tgcgctcctg gattcccaat ttcgttagct tgacctacct gcaaaatcga ggtgcagcct
     2221 tttctatctt acaagatcag cagctgttat tcgctgtcat tactctggtt gtcgtgatag
     2281 gtgccatttg gtatttacat aaacacatgg aggactcatt ctggatggtc ttgggtttga
     2341 ctctaataat cgcgggtggt cttggaaact ttattgacag ggtcagtcag ggctttgttg
     2401 tggatatgtt ccatcttgac tttatcaact ttgcaatttt caatgtggca gatagctatc
     2461 tgacggttgg agtgattatt ttattgattg caatgctaaa agaggaaata aatggaaatt
     2521 aaaattgaaa ctggtggtct gcgtttggat aaggctttgt cagatttgtc agaattatca
     2581 cgtagtctcg cgaatgaaca aattaaatca ggccaggtct tggtcaatgg tcaagtcaag
     2641 aaagctaaat acacagtcca agagggtgat gtcgtcactt accatgtgcc agaaccagag
     2701 gtattagagt atgtggctga ggatcttccg ctagaaatag tctaccaaga tgaggatgtg
     2761 gctgtcgtta acaaacctca gggaatggtt gtgcacccga gtgctggtca taccagtgga
     2821 accctagtaa atgccctcat gtatcatatt aaggacttgt cgggtatcaa tggggttctg
     2881 cgtccaggga ttgttcaccg tattgataag gatacgtcag gtcttctcat gattgctaaa
     2941 aacgatgatg cgcatctagc acttgcccaa gaactcaagg ataaaaagtc tctccgcaaa
     3001 tattgggcga ttgttcatgg aaatctgcct aatgatcgtg gtgtaattga agcgccgatt
     3061 ggccggagtg aaaaagaccg taagaaacag gctgtaactg ctaaagggaa gcctgcagtg
     3121 acgcgttttc acgtcttgga acgctttggc gattatagct tagtagagtt gcaactggag
     3181 acagggcgca ctcatcaaat ccgtgtccac atggcttata tcggccattc agtcgctggt
     3241 gatgaggtct atggtcctcg caagactttg aaaggacatg gacaatttct tcatgccaag
     3301 actttaggtt ttactcatcc gagaacaggt aagaccttgg aatttaaagc agatatccca
     3361 gagattttta aggaaacctt ggagagattg agaaagtaag aatgaaaaag aaattaacta
     3421 gtttagcact tgtaggcgct tttttaggtt tgtcatggta tgggaatgtt caggctcaag
     3481 aaagttcagg aaataaaatc cactttatca atgttcaaga aggtggcagt gatgcgatta
     3541 ttcttgaaag caatggacat tttgccatgg tggatacagg agaagattat gatttcccag
     3601 atggaagtga ttctcgctat ccatggagag aaggaattga aacgtcttat aagcatgttc
     3661 taacagaccg tgtctttcgt cgtttgaagg aattgggtgt ccaaaaactt gattttattt
     3721 tggtgaccca tacccacagt gatcatattg gaaatgttga tgaattactg tctacctatc
     3781 cagttgaccg agtctatctt aaaaaatata gtgataatcg tattactaat tctgaacgtc
     3841 tatgggataa tctgtacggt tatgataagg ttttacagac tgcttcagaa aaaggtgttt
     3901 cagttattca aaatatcaca caaggggatg ctcattttca gtttggggac atggatattg
     3961 agctctataa ctatgaaaat gaaaccgatt catcgggtga attaaagaaa atttgggatg
     4021 ataattccaa ttccttgatt agtgtggtaa aagtcaatgg caagaaaatt taccttgggg
     4081 gcgacttaga taatgttcat ggagcagaag acaagtatgg tcctctcatt ggaaaagttg
     4141 atttgatgaa gtttaatcat catttagata caaaaaattc taatacaaaa aattttataa
     4201 aaaatttgtc tcctgagata attgttcaaa cttcctcctc accaattggt tcgtatgata
     4261 gcggtcaaga aataaatagg gaatatatta gttggcttga atcaatggga attcaacata
     4321 taaatgcagc cagcaaagac tatgatgcaa cagtttttga tattcgacaa gacggtttag
     4381 taaacatttc aacttcctac aagccgattc caagttttca agctggttgg cataagagtt
     4441 catatggaaa ctggtggtat caagcgcctg attctacagg agaatatgct gtcggttgga
     4501 atgaaattaa gggtgaatgg tattacttta accaaacggg tatcttatta cagaatcaat
     4561 ggaaaaaatg gaacaatcgt tggttctatt tgacagactc tggtgttgct gctaaaaatt
     4621 ggaagaaaat cgctggaatc tggtattatt ttaacaaaga aaaccagatg gaaattggtt
     4681 ggattcaaga taaagagcag tggtattatt tggatgttga tggctctatg aagacaggtt
     4741 ggcttcaata taaggggcaa tggtattact ttgctccatc aggggaaatg aaaacgggct
     4801 gggtaaaaga taaagaagcc tggtactata tggattctac tggtatcatg aagacaggtg
     4861 agatagaagt tgctggacat cattactatc tagaagaatc aggagctatg aagcaaggct
     4921 ggcttaaaaa ggcaaatgat tggtatttct acaagacaga tggttcacga gctataggtt
     4981 ggattaaaga caaggataaa tggtacttct tgaaagaaaa tggtcaatta cttgtgaatg
     5041 gtaagacacc agaaggctat actgtggatt caagtggtgc ctggttagtg gatgttccga
     5101 tcgagaaatc tgctacaatt aaaactacaa gtcattcaga aataaaagaa tccaaagaag
     5161 tagtgaaaaa ggatcttgaa aataaagaaa cgagtcaaca tgaaagtgtt acaaattctt
     5221 caactagtca agatttgaca tcctcaactt cacaaagctc tgaaacgagt gtaaacaaat
     5281 cggaatcaga acagtagtag aaaagaaggt tttagggcct tctttttcct atcaactctt
     5341 ttctatttcc tgttattcat gttataatgg ataaatatga ataatcggag tgagactatg
     5401 aaatacaaac ggattgtctt taaggtgggt acttcttctc tgacaaatga ggatggaagt
     5461 ttatcacgta gtaaggtaaa ggatattacc cagcagttgg ctatgctgca cgaggctggt
     5521 catgagttga ttttggtgtc ttcaggtgcc attgcggctg gttttggagc cttaggattt
     5581 aaaaagcgtc cgactaaaat tgctgataaa caggcttcag cagcggtagg gcaggggctt
     5641 ttgttggaag aatatacaac caatcttctc ttgcgtcaaa tcgtttctgc acaaatcttg
     5701 ctgacccaag atgactttgt ggataagcgt cgttataaaa atgcccatca ggctttgtcg
     5761 gttttgctca accgtggggc aattcctatc atcaatgaga atgatagtgt cgttattgat
     5821 gaggtcaagg ttggggacaa tgacactcta agtgcccaag tagcggcgat ggtccaagca
     5881 gaccttttgg ttctcttgac agatgtggac ggtctctata ctggaaatcc taattcagat
     5941 ccaagagcca aacgcttgga gagaatcgag accatcaatc gtgagattat tgatatggct
     6001 ggtggagctg gttcgtcaaa cggaactggg ggtatgttaa ccaaaatcaa ggctgcaact
     6061 atcgcgacgg aatcaggagt tcctgtttat atctgctcat ccttgaaatc agattccatg
     6121 attgaggcgg cagaggagac cgaggatggt tcttactttg ttgctcaaga gaaggggctt
     6181 cgtacccaga aacaatggct tgccttctat gctcagagtc aaggttctat ttgggttgat
     6241 aaaggggctg cggaagctct ctctcaacat ggaaagagtc ttctcttatc tggtatcgtt
     6301 gaagcagaag gagtcttttc ttacggtgat atcgtgacag tatttgacaa ggaaagtgga
     6361 aaatcacttg gaaaaggaca cgtgcaattt ggagcatctg ctttggagga tatgttgcgt
     6421 tctcaaaaag ccaagggtgt cttgatttac cgtgacgact ggatttccat tactcctgaa
     6481 atccaactac tttttacaga attttagagg taaactatgg tgagtagaca agaacaattt
     6541 gaacaggtac aggctgttaa aaaatcgatt aacacagcta gtgaagaagt gaaaaaccaa
     6601 gtcttgctag ccatggctga tcacttagtg gctgctactg aggaaatttt agcggctaat
     6661 gccctcgata tggcagcggc taaggggaaa atctcagatg tgatgttgga tcgtctttat
     6721 ttggatgcag atcgtataga agcgatggca agaggaattc gtgaagtggt tgccttacca
     6781 gatccaatcg gtgaagtttt agaaacaagt cagcttgaaa atggtttggt tatcacaaaa
     6841 aaacgtgtag ctatgggggt catcggtatt atctatgaaa gccgtccaaa tgtgacgtct
     6901 gatgcggctg ctttgactct taagagtgga aatgcggttg ttcttcgtag tggtaaggat
     6961 gcctatcaaa caacccatgc cattgtcaca gccttgaaga agggcttgga gacgactact
     7021 attcatccaa atgtgattca actggtggag gatactagcc gtgaaagtag ttatgctatg
     7081 atgaaggcca agggctatct agaccttctc attcctcgtg gaggagctgg cttgattaat
     7141 gcagtagttg agaatgccat tgtgcctgtt atcgagacag gaactgggat tgtccatgtt
     7201 tatgtcgata aggacgcaga tgacgacaag gcactgtcta tcatcaacaa tgccaaaacc
     7261 agtcgtcctt ctgtctgcaa tgccatggag gttctgctgg ttcatgaaga caaggcagca
     7321 agcttccttc ctcgcttgga gcaagtgctg gttgcagatc gaaaagaagc tgggttggaa
     7381 ccaattcaat tccgcctaga tagcaaagca agccagtttg tttcaggtca agctgctcaa
     7441 gcacaagact ttgataccga gtttttagac tatattctag ctgttaaggt tgtgagcagt
     7501 ttagaagaag cggttgcgca tattgaatcc cacagtaccc atcattcgga tgctattgtg
     7561 acggaaaatg ctgaagctgc agcatacttt acagatcaag tggactctgc agcggtgtat
     7621 gttaatgcct caactcgttt cacagatgga ggacaatttg gtcttggttg tgaaatgggg
     7681 atttctactc agaaattgca cgcgcgtggt ccaatgggct tgaaagagtt gaccagctac
     7741 aagtatgtgg ttgctggtga tgggcagata agggagtaag agatgaagat tggatttatc
     7801 ggtttgggga atatgggtgc tagcttggca aaatctgtct tgcagactag gacgtcagat
     7861 gagattctcc ttgccaatcg tagtcaagct aaggtagatg ctttcattgc agactttggt
     7921 ggtcaggctt ccagcaatga agaaatgttt gcagaagcag atgtgatttt tctaggagtt
     7981 aagcctgctc agttttctga actgctttct caatatcaga ccatccttga aaaaagagaa
     8041 agtcttcttt tgatttcgat ggcagctgga ttgaccttag aaaaactagc aagtcttatc
     8101 ccaagccaac accgaattat tcgtatgatg cctaataccc ctgcttctat cgggcaagga
     8161 gtgattagtt atgccttgtc tcctaattgc agggctgagg acagtgagat cttttgtcag
     8221 cttttagcca aggctggtct cttggttgaa ttaggagaaa gcttaatcaa tgcagcgaca
     8281 ggtcttgcag gttgtggacc agcctttgtc tatctcttta ttgaggcctt ggcagatgca
     8341 ggtgttcaga cgggattacc acgagaaaca gccttgaaaa tggcagccca aactgtggta
     8401 ggagctgggc aattggtcct agaaagccag caacatcctg gagttttgaa agatcaagtt
     8461 tgtagtccag gcggttcgac tattgctggc gtagcaagcc tagaagcgca tgcttttcga
     8521 ggcacagtca tggaggcagt tcatcaagcc tataaacgaa cacaagaact aggtaaataa
     8581 gaggtagttt tatctgcctc ttttatggtg gttgatactc ttcgaaaatc aaattcaaac
     8641 tgcgtcagct tcaccttgcc gtactcaagt acagcctgcg gctagcttcc tagtttgctc
     8701 tttgattttc attgagtatg aaatgagaag acaaaaagat tgtcacaaac ccctattttt
     8761 ttgatagaat agaagtagta aaaaagaaat gagttagaca tgtcaaaagg atttttagtc
     8821 tctcttgagg gaccagaggg agcaggcaag accagtgttt tagaggctct gctaccaatt
     8881 ttagaggaaa aaggagtaga ggtgttgacg acccgtgaac ctggcggagt cttgattggg
     8941 gagaagattc gggaagtgat tttggatcca agtcatactc agatggatgc taaaacagag
     9001 ctacttctct atattgccag tcgcagacag catttggtgg aaaaagttct tccagccctt
     9061 gaagctggca agttggtcat catggatcgt tttatcgata gttctgttgc ctatcaggga
     9121 tttggtcgtg gcttagatat tgaagccatt gactggctca atcagtttgc gacagatggg
     9181 ctcaaacccg atttgacact ctattttgac atcgaggtgg aagaagggct ggctcgtatt
     9241 gctgcgaata gtgaccgcga ggttaatcgt ttggatttgg aagggttgga cttgcataaa
     9301 aaagttcgtc aaggctacct ttctcttctg gataaagagg gaaatcgcat tgtcaagatt
     9361 gatgctagtc tccctttgga gcaagttgtg gaaactacca aggctgtctt gtttgacgga
     9421 atgggcttgg ccaaatgaaa caagatcaac taaaggcttg gcaaccagct cagtttgacc
     9481 gttttgtccg tatcttagaa caagaccagc tcaatcacgc ctatctcttt tcaggtttct
     9541 ttggaagctt ggaaatggcg caatttttag ctaagagcct cttttgtacg gataaagttg
     9601 gcgtcttacc atgtgagaaa tgccgaagtt gcaagctgat tgaacaggaa gagtttccag
     9661 atgtcacctt gattaagcca gtcaatcagg tcatcaagac agaacgcatt cgggaattgg
     9721 tgggacagtt ttctcaagca gggattgaaa gccagcaaca ggtctttatt atcgagcaag
     9781 cggataaaat gcatcccaac gcagccaatt ctctgctcaa ggtcatcgaa gaaccccaga
     9841 gtgaagttta tattttcttc ttgactagcg atgaggaaaa gatgttaccg acaatccgaa
     9901 gtcggactca gatcttccac tttaaaaagc aagaagaaaa acttatctta ctcttagaac
     9961 aaatgggact tgttaagaaa aaagcgactc ttttagctaa gtttagtcaa tcgcgagctg
    10021 aagcagaaaa gttggctaat caggcaagtt tttggacctt ggtcgatgaa agtgaacgcc
    10081 tgctgacttg gttagtagct aagaaaaaag aaagttatct acaggttgcc aaattagcca
    10141 acttggcaga tgataaggaa aaacaggatc aggttttacg gattcttgaa gttctctgtg
    10201 ggcaggacct cttgcaggta agagtaagag tgattctaca agatttacta gaagctagaa
    10261 aaatgtggca agctaatgtc agctttcaaa atgccatgga atatctggtc ttgaaagaaa
    10321 tataaactca aaaatgaatg ataaagaaag gaaagggctg ttttatggac aaaaaagaat
    10381 tatttgacgc gctggatgat ttttcccaac aattattggt aaccttagcc gatgtggaag
    10441 ccatcaagaa aaatctcaag agcttggtag aagaaaatac agctcttcgc ttggaaaata
    10501 gtaagttgcg agaacgcttg ggtgaggtgg aagcagacgc tcccgtcaag gccaagcatg
    10561 ttcgcgaaag tgtccgtcgt atttaccgtg atggatttca cgtatgtaat gatttttatg
    10621 gacaacgtcg agagcaggac gaagaatgta tgttctgtga tgagttgtta tacagggagt
    10681 aagcatgcaa attcaaaaaa gttttaaggg gcagtctccc tatggcaagc tgtatctagt
    10741 ggcaacgccg attggcaatc tagatgatat gacttttcgt gctatccaga ccttgaaaga
    10801 agtggactgg attgctgctg aggatacgcg caatacaggg cttttgctca agcattttga
    10861 catttccacc aagcagatca gttttcatga gcacaatgcc aaggaaaaaa ctcctgattt
    10921 gattggtttc ttgaaagcag ggcaaagtat tgctcaggtc tctgatgccg gtttgcctag
    10981 catttcagac cctggtcatg atttggttaa ggcagctatt gaggaagaaa ttgcagttgt
    11041 tacagttcca ggtgcctctg caggaatttc tgccttgatt gccagtggtt tagcgccaca
    11101 gccacatatc ttttacggtt ttttaccgag aaaatcaggt caacagaagc aattttttgg
    11161 ctctaaaaaa gattatcctg aaacacagat tttttatgaa tcacctcatc gtgtagcaga
    11221 cacgttggaa aatatgttag aagtctacgg tgaccgctcc gttgtcttgg tcagggaatt
    11281 gaccaaaatc tatgaagaat accaaagagg tacaatttct gaattgctgg aaagcatctc
    11341 tgaaacgtct ctcaagggtg aatgtcttct gattgttgaa ggtgccagca aaggtgtgga
    11401 agaaaaagat gaggaagact tgttcttaga aatccaagct cgtatccagc aaggtatgaa
    11461 gaaaaatcaa gctattaagg aagtagctaa gatttaccag tggaataaga gtcaactcta
    11521 cgctgcctac cacgactggg aagaaaaaca ataaagggag ataggatgta ataattctgt
    11581 ctgtttctgt ttaacttaat tagtgatgat aatataaaga tgtatcactt ggtatagaag
    11641 ctttggtatt aagtttttta ttaagcccat acggaatacc gatggttgga gcagcagtta
    11701 tagcgttctt agaaggtata aatagaaaaa taaggtcatt ttaaatcaaa ggattgataa
    11761 atcagaaaga aggtgatttt ttgcgaacat acgaaaataa agaagaacta aaagctg
//
LOCUS       DAVKAO010000049        11720 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKAO010000049 DAVKAO010000000
VERSION     DAVKAO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.049"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            115..768
                     /locus_tag="VWX16_001487"
     CDS             115..768
                     /locus_tag="VWX16_001487"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9158816.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            778..1242
                     /locus_tag="VWX16_001488"
     CDS             778..1242
                     /locus_tag="VWX16_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9158817.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTADLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1255..2523
                     /locus_tag="VWX16_001489"
     CDS             1255..2523
                     /locus_tag="VWX16_001489"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9158818.1"
                     /translation="MLLIKNGHVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFKAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2537..2797
                     /locus_tag="VWX16_001490"
     CDS             2537..2797
                     /locus_tag="VWX16_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158819.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2808..4169
                     /gene="pdrM"
                     /locus_tag="VWX16_001491"
     CDS             2808..4169
                     /gene="pdrM"
                     /locus_tag="VWX16_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9158820.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4323..5291
                     /locus_tag="VWX16_001492"
     CDS             4323..5291
                     /locus_tag="VWX16_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9158821.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5307..6299
                     /locus_tag="VWX16_001493"
     CDS             5307..6299
                     /locus_tag="VWX16_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9158822.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6518..7561
                     /locus_tag="VWX16_001494"
     CDS             6518..7561
                     /locus_tag="VWX16_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9158823.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMNLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7625..9310
                     /gene="lpdA"
                     /locus_tag="VWX16_001495"
     CDS             7625..9310
                     /gene="lpdA"
                     /locus_tag="VWX16_001495"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9158824.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGSASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVVGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDNIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9374..10363
                     /locus_tag="VWX16_001496"
     CDS             9374..10363
                     /locus_tag="VWX16_001496"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9158825.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10428..11498)
                     /gene="xerS"
                     /locus_tag="VWX16_001497"
     CDS             complement(10428..11498)
                     /gene="xerS"
                     /locus_tag="VWX16_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9158826.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLKSGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGIRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3471 a         2208 c         2638 g         3403 t
ORIGIN      
        1 cacgtattca aatcgtatat agtcgattta ttaaatcttt agataaattg taaaaagtgg
       61 tttccatagc cactttttgc tataatagag gcagtaaaaa tgaaggagtc agctatggaa
      121 cactcgtctt ggcatgcttt gattaaggcg caattacctg agggttattt cgggaaaatc
      181 aatcagttta tggagcaggt ctattctcag gggattattt atccacccaa ggaaaaggtt
      241 tttcaggctc tcttgacaac actgcttgaa gaagttaagg tggtaattct agggcaagac
      301 ccctatcacg gaccaggtca agcgcagggc ttgagttttt ctgtacctga ctctatccca
      361 gctccaccat ccttgcaaaa tatcttgaaa gaattgtcag atgatatcgg agttaagaaa
      421 tctcatgatt tgacagcttg ggctgagcaa ggagtcttgc ttcttaatgc ttgtttgact
      481 gttcctgctg gacaggccaa tggtcatgct ggtcaaatct gggagccttt tactgatgct
      541 gtgattcagg tggtcaatca tctagataga ccagtcgttt ttgtactctg gggagcttat
      601 gcacgtaaga agaaggcctt agttaccaat cctcatcact tgattatcga atcagcccat
      661 ccaagtcctt tgtcggttta tagaggattt tggggttcca agcccttttc caaagccaat
      721 gcattcttaa aagagacagg acaagagcca atcgattggc ttagataagg agaaaatatg
      781 cctcagttag cgacgatttg ctacattgat aatgggaaag aactgctcat gctccatcgt
      841 aataagaaac ccaatgatgt ccatgaaggg aaatggattg gtgtgggtgg taagctagag
      901 agaggagaga cgccccagga atgcgcggcg cgtgaaatcc ttgaagaaac agggctcaaa
      961 gccaagccag ttctaaaagg tgtcatcact tttcctgaat ttacagcaga tttagactgg
     1021 tacacctatg tttttaaggt gacggagttt gagggcgact tgattgactg caatgagggg
     1081 acgctagaat gggttcccta tgacgaggtt ttgagcaagc cgacttggga aggtgaccac
     1141 acctttgttg agtggctttt agaggataaa cccttctttt cagccaagtt tgtttatgat
     1201 ggggataaat tgttggatac ccaagttgat ttctatgaat aaaggagaaa gcagatgcta
     1261 ctaatcaaaa atggtcatgt aatggatccc aagtctggtt tggatcaagt ttgtgatgtc
     1321 ttagttcaag atgggaaaat tatcaaaatt gcgcctgaga tcacggaaga aggagcagaa
     1381 acgattgatg ctactggtct tgtggttgct cctggtttgg tcgatattca tgtgcatttc
     1441 cgtgaacctg gtcaaacaca taaagaagac attcatactg gtgccctagc agccgctgca
     1501 ggtggtttta ctactgtcgt catgatggct aataccagtc caaccatttc agacgtggag
     1561 actttgcaag cagttctcca gtcagctgcc aaagagaaga ttaatgtcaa gacagttgcg
     1621 accattacta aaaactttaa tggtaaaaac ttgactgact ttaaggcgct cttagaagct
     1681 ggtgcggttg gtttctctga tgacggtatt ccgcttgaga gcagtaagat tgtcaaggaa
     1741 gccatggagg aagccaaaaa actcaatacc tttatcagcc ttcatgagga agatccaggt
     1801 ttgaacggtg ttcttggctt taatgaaaat attgctagag aacatttcca tatctgtggt
     1861 gctactgggg tggctgagta cgctatgatg gcgcgtgatg tcatgattgc ctatgcaact
     1921 aaagcccatg ttcacatcca gcatttgtct aaggaagaaa gtgttaaagt agtggagttt
     1981 gctcaggggt taggtgcaga agtcacagca gaagtagcac cacagcattt ctctaagaca
     2041 gaagcacttc ttttaacaca aggtagcaat gctaagatga atccaccgct tcgtttggaa
     2101 tcagaccgtc gtgccgttat cgaaggtctc aaatcaggtg tcatcacagt tattgcgact
     2161 gaccacgcgc ctcatcatgt agatgaaaaa aatgttgaag atattaccaa agcgccatct
     2221 ggtatgactg gcttagaaac atccctgtct ctcggcttga cctatttggt agaagctggt
     2281 gagttgagct tgatggaatt acttgaaaaa atgacataca acccagccaa gctttacaac
     2341 tttaaagcag gttacttggc tgagaatggt ccagcagata tcactatttt tgatgccaag
     2401 gctgaccgcc ttgtggactc ccattttgct tccaaagcag ctaattcacc attcatcggt
     2461 gaaaccttaa aagggcaggt taaatatacc atctgtaagg gacaaatcgt ctatcaagct
     2521 tgataggagt tagaatatga attattttcg aaaacgtagg gagagacaag cgaaatctaa
     2581 tagtggaatt tactgtcccg cagctaatcg ttccaaaatt caatatgaaa ctaaagaaaa
     2641 agctgaccat gcggttcaat atgataacgg aggtttagtt agaagctatt attgtcgtac
     2701 atgtgcgtgt tggcatacta cttcaaaatc taataaacct ccgctaagtt tgaaaaacta
     2761 tggtctgaaa ctctttggtt tagataaatc agaatagaaa gagaagtatg tataagacaa
     2821 agtgtttacg agagaagtta gtattatttt taaaaatttt cttcccaatc ctgatctacc
     2881 aatttgccaa ttattctgcc tcttttgttg atactgcaat gacaggtcaa tacaacacta
     2941 tggacttggc tggtgtatct atggcaacca gtatctggaa tcctttcttt acatttctaa
     3001 cagggattgt gtcagccttg gtgcctatca ttggtcacca tcttggtcga ggcaaaaagg
     3061 aagaagttgc gtctgatttt taccaattta tttatttggc cttgggccta tctgtggtct
     3121 tgctggggat ggtacttttc ttggcaccaa caatcttgaa tcatattggg ttagaagcag
     3181 cagtagcggc agtagcggtt cgctatcttt ggtttttatc tatcgggatt atccccttgt
     3241 tgctctttag cgtcattcgt tccttgctgg attcgctggg cttgaccaaa ctgtccatgt
     3301 acctcatgct tttgttactc cctctcaata gcggatttaa ctatctcttg atttacggtg
     3361 cctttggtgt tccagaactg ggaggggctg gtgctggttt aggaacatcc ttggcctact
     3421 gggtcttgct tgggatttct gttctggttt tatttaaaca ggagaagctc aaagccttac
     3481 accttgagaa acgaattcca cttaatatgg ataacattaa ggaaggagtt cgtttaggtc
     3541 tgcctattgg gggaactgtc ttcgcggaag tggctatctt ttcagtggtt ggcttgatta
     3601 tggctaagtt ttcgtccttg attatagcta gtcaccagtc agctatgaac ttttcaagtc
     3661 ttatgtacgc ctttcctatg agtatctcat cggctatggc tattgtcgtt tcctatgaag
     3721 tgggagccaa gcgatttgat gatgcgaaaa cctatattgg tctaggaaga tggactgctc
     3781 tcatttttgc ggccttcacc ctaaccttcc tttacatttt taggggaaat gtggccagtc
     3841 tttatggtaa cgacccaaaa tttatcgatt tgacagcgcg ttttttaact tatagtcttt
     3901 tcttccagtt agcagatacc tttgcggcac cgcttcaggg aattttgcgg gggtataagg
     3961 atacagttat tcctttttac cttggtttgc ttggttattg gggcgtagca atccctgtgg
     4021 ctacgctatt tgattcccta acagattttg gagcttattc ttactggatc ggcttgatta
     4081 ttagtttgat tgtgagtggg gcgctctacc gttggcgttt aactgtgatt atgaagagat
     4141 ttgaatcttt agcaaaatcc aaacgctaaa aagtttgtga aaaaatatcc ttgacaagaa
     4201 aatcccttgt tttcacgggg ttttcttttt tattttgtga aatttctttt agaaaaactt
     4261 tgacagtatt tgccaaaagc ggtaaaatag taaggtaaga agaaagttag aaaggcaaga
     4321 agatgtcaac tttagataaa aatcttttgc tagaaatgtt ccgtaagatg gaagaaatcc
     4381 gtcgcatgga cttaaaaatt gcacaattag taaagaaagg gaaagtgcca ggaatgacgc
     4441 acttttctgt tggtgaagag gcagctaacg tgggggctat gttagctctc aatccagatg
     4501 atctgattac ctcaaaccac cgtggacacg ggcaagctat tgctaaaggg attgacctca
     4561 acggaatgat ggctgaaatc cttggtaaat acactggaac ctgtaaaggg aaaggtggat
     4621 ctatgcatat cgctgacctt gatgctggga accttggtgc caatggtatc gtaggtggtg
     4681 gtatggggat cgctgtcggt gcagccctca gtcagcaaat gcaaaatacc ggtaaaatcg
     4741 ttgtctgctt ctttggagat ggtgcgacca atgaaggtgt tttccacgaa gcagtgaaca
     4801 tggcttctat ctggaacctg ccagtcattt tctattgcat taacaacggt tacggtatct
     4861 ctgcggatat caagaaaatg accaatatag aacatatcca tcaacgtagc gccgcttatg
     4921 gaattcctgg aatgttcatc gaagacggta acaatgtcat cgatgtctat gaaggatttc
     4981 agaaagctgt agaccatgtt cgcagtggca atggtccagt cttgattgaa agtgtaactt
     5041 atcgctggct cggtcactca tcatctgacc ctggtaaata tcgtacgcgt gaggaagtgg
     5101 aattgtggaa acaaaaagat ccaatcgaaa acctccgcaa ttaccttatt gaaaataaca
     5161 ttgcaagtgc cgaagaattg gaagaaatcc aagcgcaagt aaaggaagca gtagaagctt
     5221 ctgttaaatt tgcagaggaa agcccattcc caccgcttga atcagccttt gaagatattt
     5281 acgcagacta aggagaaaga gataaaatgg aaacaaaaac aatgtccttc cgtgacacca
     5341 ttatccttgc tatgtctgag gaaatgcgtc gcgatgaaaa tgtgttcttg atgggagaag
     5401 acgtcggtgt cttcggagga gacttcggta cttctgttgg aatgcttgaa gaatttggtc
     5461 cagaacgtgt ccgtgactgt ccgatttctg aagctgccat ctctggagct gcagcaggag
     5521 cagccatgac aggacttcgt ccaatcgtcg atatgacctt catggacttc tctgttattg
     5581 ccatggacaa tatcgtcaac caagctgcta aaacacgtta catgtttggt ggtaaaggtc
     5641 aggttccaat gactgttcgt tgtgcagctg gtaacggagt tggctctgcc gcccagcact
     5701 cacaatctct agagtcttgg tttactcaca ttccaggact taaggttgtg gcacctggaa
     5761 cacctgcgga catgaaaggc ctgcttaagt cttctatccg tgataacaac ccagttatta
     5821 tacttgagta caagtcagaa tttaaccaaa aaggggaagt gccagttgat ccagactaca
     5881 caattccact tggggttggt gaaatcaaac gccaaggaac ggatgtaaca gttgtcactt
     5941 atggaaaaat gcttcgccgt gtggttcaag ctgctgaaga attagctgaa gaaggaattt
     6001 cagttgaaat tgttgaccca cgtacccttg ttccgcttga taaggatatc atcataaact
     6061 cagtgaagaa gactggtaag gttgttctgg tcaacgatgc ccacaaaaca agtggctata
     6121 tcggtgaaat ttcagctatt atttcagaat cagaagcatt tgactatcta gatgcaccaa
     6181 tccgccgttg tgcaggagaa gatgtgccga tgccttatgc acaaaaccta gaaaatgcaa
     6241 tgattccaac agttgaaagt atcaaagatg caatccgaaa aacttataac aaagaataga
     6301 gttacataag ataagttatg taaataaaac aaagctgacg agaaactttg tatcttttag
     6361 gtgcagagct cttcgtcctt aatatcttag attagagcac gggctaaaag cttggaaaaa
     6421 aagataaatc tccttggctt catcgaagcc atcgtcgatt tcctattttt cagtcgcttt
     6481 tgacgccctt tgtatcataa atagaattgg agaggtcatg gctgatgaca agctaagagc
     6541 gactcctgca gctagaaagt tagcggatga tttagggata aacctctacg acgtttctgg
     6601 ctcaggtgca aacggtcgtg tccacaaaga agacgtggaa acttataaag acacaaacgt
     6661 ggttcgcatt tcgccacttg caaaacgaat tgccctcgaa cataacattg cttggcagga
     6721 aatccaagga accggtcatc gtggtaaaat catgaagaag gatgttttgg ccctgcttcc
     6781 tgaaaatatc gaaaacgata gcatcaaatc tcctgctcag attgaaaaag tggaagaagt
     6841 tcctgataac gtaacaccat atggtgaaat cgagcgtatt ccaatgacac caatgcgtaa
     6901 ggttattgcc caacgcatgg ttgaatctta cttgactgcg ccaaccttca ccctcaacta
     6961 tgaagttgat atgactgaaa tgttggctct tcgtaagaag gttcttgagc caatcatgga
     7021 agcaactggg aagaagacta ctgtaacaga ccttctttcg cttgcagttg ttaagactct
     7081 tatgaaacac ccatacatca acgcttcatt gacagaagat ggcaagacta ttatcactca
     7141 caactatgtc aatcttgcca tggcagttgg gatggataat ggattgatga cacctgttgt
     7201 ttacaatgct gagaagatga gtctttcaga actggttgta gcctttaagg atgttattgg
     7261 ccgtaccttg gatggtaaat tggctccaag tgagttacaa aattcaacat tcacaatcag
     7321 taatttggga atgtttggtg ttcagtcctt tggtccgatt attaaccaac ccaactcagc
     7381 tatccttggt gtcagttcga caatcgagaa gccagttgtc gtcaatggtg aaattgtgat
     7441 tcgcccaatc atgaatttag gattaaccat tgaccaccgt gtcgtagatg gtatggctgg
     7501 tgctaagttt atgaaggact tgaaagagtt gattgaaacg ccaatctcaa tgttgattta
     7561 agagcaagag tattcatttt taagatatag ataaaggaag gaagacatgg ccttagaagt
     7621 aattatgcca aaagccggcg tggatatgac agaaggacaa atcgtccaat ggaataaaaa
     7681 agtcggagaa tttgtaaaag aaggagaaat ccttttggaa atcatgactg ataaagtaag
     7741 catggaattg gaagccgaag aagatgggta cttgattgcc attctcaaag gagatggtga
     7801 aactgtccct gtaacggaag ttatcggtta ccttggtgaa gaaagggaaa acatcccaac
     7861 agctggatca gcatcgccag aagctagccc tgtccctgta gcaagtacct caaacgatga
     7921 tggtaagagc gatgatgctt ttgatatcgt tgtgattggt ggaggtcctg ctggttatgt
     7981 tgcagccatt aaagctgccc aactcggcgg taaggttgcc cttgttgaga aatctgaact
     8041 tggtggaacc tgcttaaacc gtggatgtat tccaaccaag acctaccttc ataacgctga
     8101 aattattgaa aatatcggtc atgctgcaaa tcgtggtatc gtgattgaaa atcctaactt
     8161 cactgttgat atggaaaaac ttttagaaac taaatctaaa gttgttaata ctcttgttgt
     8221 tggtgttgca ggacttcttc gtagctacgg agttactgtt cataaaggaa ttggtacaat
     8281 cactaaagac aagaacgtct tggtaaatgg ttctgaattg cttgaaacca agaagatcat
     8341 tcttgctggt ggttcaaaag tcagcaagat caacgtccct ggtatggaat ctccacttgt
     8401 catgactagt gatgacattc ttgaaatgaa cgaagtgcca gaaagccttg ttatcatcgg
     8461 tggtggagtt gtcggtatcg aactcggtca ggccttcatg acatttggtt caaaagtgac
     8521 tgttatcgaa atgatggacc gtatagttcc agctatggat gcggaagttt ctaagaacct
     8581 tcgcttgatc cttgagcgta aaggaatgac catcttgact ggtactaaac tgcaagaaat
     8641 cattgaggaa aatggtcaac ttcgtatcaa ggttgaagga aaagacaata tcatcgcaag
     8701 caaagctctt ctttcaattg gtcgtatgcc agaccttgaa ggtattggag aggttgagtt
     8761 tgaattggat cgtggttgta tcaaggtcaa tgaatacatg gaaacttcag ttccaggcat
     8821 ttatgcacca ggtgacatca acggtactaa gatgttggct cacgcagctt tccgcatggg
     8881 tgaagtttcc gctgaaaatg cccttaaagg aaatcatgca gttgccaaat tgaatttgac
     8941 tcctgcagcc atctacactc tccctgaagt agcagcagta ggtttgacag aagaacaagc
     9001 ccgtgagaaa tacgatgttg ccatcggtaa gtttaacttt gctgctaacg gtcgtgctat
     9061 tgcatctgac gcagctcaag gtttcgtaaa agttatcgct gataagaaat acggagaaat
     9121 ccttggtgta cacattattg gtcctgcagc cgcagaatta ataaacgaag catcaagcat
     9181 catcgaaatg gaaatcactg ttgaggaaat gctgaagacc atccacggac acccaactta
     9241 ctctgaagtg atgtacgaag cgtttgcaga tgttctagga atggccatcc attcacctaa
     9301 gaaaaaataa aacgaagata gattagactg gaaggttatt tccagtctct atcatataaa
     9361 gtaaagggat atcatgaaat acattatcaa tcattcaaat gacactgctt ttaatattgc
     9421 cttggaagaa tacgccttta aacacctttt ggatgaggat caaatcttcc tactttggat
     9481 taacaagcca tctatcattg ttggtcgtca ccagaacact atcgaagaaa tcaaccgtga
     9541 ttatgttcgc gaaaatggta ttgaggtagt tcgccgtatt agtggtggtg gagctgttta
     9601 ccacgattta aataacctca actacacgat catctccaaa gaagatgaaa ataaggcatt
     9661 tgacttcaag agcttctcaa ctccggttat caataccctg gctcaactcg gggttaaagc
     9721 tgagttcaca ggtcgtaatg accttgagat tgatggtaag aagttctgtg gcaatgccca
     9781 agcctatatc aacggccgta tcatgcacca cggttgcttg ctctttgacg ttgatttgtc
     9841 agtcctcgca aatgccctta aggtttcaaa agataaattt gaatcaaaag gtgtgaaatc
     9901 cgtccgtgcc cgtgtaacca atattatcaa tgaattacca aaaaaaatca cagtcgaaaa
     9961 attccgtgat ttactcctgg aatacatgaa aaaagagtac ccagagatga ctgaatacgt
    10021 cttttcagaa gaagaattgg ccgaaatcaa tcgcatcaag gatactaagt ttggaacttg
    10081 ggactggaac tatggtaaat cacctgaatt taacgtccgt cgtggaacca aattcactag
    10141 tggtaaggtt gaagtctttg ctaatgttac tgaatcaaaa atccaagaca tcaagattta
    10201 tggtgacttc tttggtattg aagacgttgc tgcagtagaa gatgtccttc gtggggtgaa
    10261 atacgaacgc gaagatgttc ttaaggcgct aaaaactatt gatatcacac gctacttcgc
    10321 tggtattagc cgtgaagaaa tcgctgaagc ggtagttgga taaatcaaaa agaagttggc
    10381 caacttcttt ttttgatatt tatttacata atttaaaata cgtaaaatca taaactatcc
    10441 agagcattct tttgttcatc actaacaata tgggtataga ggtcagtgac ttgtgtgcta
    10501 gaatgtccta gttggtgact gactaaaact tgtgatttag tcgcatcata gagcctagtt
    10561 gctagtgtat ggcgcagttt atggggtgtt acacgcactt taaaatcctc tgagtattta
    10621 gcaaccattt tctcaacgct agaagcatcg atacgattag gaacacctct gtagagagtt
    10681 aaaaaaaggg ctgtatctgt tttttccgtt ttatagcgtt gattccgaat ggccagataa
    10741 ttctctaaat aaggtttagc aaaagcagcg acattgactg agtcacgttt gccacctttt
    10801 cgagtaacat caataaccat catttttaga ttgagatctc ttagatctag attaacagct
    10861 tcagataagc gaataccaga tgccaagaga agggcaataa tggctaaatc tcgttcttta
    10921 tttttgttga atgatgagag agctcgattt gaaagctgtt gtgggtgctc ttgatcgata
    10981 taagttagaa aaccttctgt ttcatcacct agaaagagtt tttgcttgat attttcagct
    11041 ctggcagcaa gggtttcttt ctttttcttg gttgaaactt ttttcattac attacgatag
    11101 aaataaggtt ccccctgatc gttttcaacc tcctcggtta gatacttgta aagactagaa
    11161 agtgctgata aggttcgatt gatagttgtc tgtgaaacac cctgttttgt tgtattagca
    11221 ttcagcaagg gacgttcacg tagataaagg ataaaggatt ccatgtcttt cttagacata
    11281 ttttccaaaa ctgataaagg aatatcggat attttatcag cgtttgaaat accagacttc
    11341 aaaacccagc tgaaaaatcg gtcatattcc ttaaggtatt cgtacagggt tgtaaaactg
    11401 tagggtacag ccagcttaga ttggtagtat tccagaacat accagggcat gagttgtttt
    11461 agtttgtcga ttcgttccag taaaatctca cgtttcatgt attttctcca taagtaagta
    11521 tactagtagt atagcataga cttgtatatt tttcaagaaa attatagttt ttcagaaaga
    11581 tgttatagga gtttttcaaa gtgataaaaa cacttagacc tacaaacaaa acagaataag
    11641 gaaaatctca cccactaaac ttctgttaat actacataac tgaagagcta tataaggagc
    11701 tatgagatac aaaacacatt
//
LOCUS       DAVKAO010000050        11663 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKAO010000050 DAVKAO010000000
VERSION     DAVKAO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11663
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.050"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(142..1773)
                     /locus_tag="VWX16_001498"
     CDS             complement(142..1773)
                     /locus_tag="VWX16_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9158827.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2066..7006
                     /locus_tag="VWX16_001499"
     CDS             2066..7006
                     /locus_tag="VWX16_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9158828.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFVGPALAEETAVPE
                     NSGANTELVSGESEHSINEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKAYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEAQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKSAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7096..8292
                     /locus_tag="VWX16_001500"
     CDS             7096..8292
                     /locus_tag="VWX16_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9158829.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQSG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8428..8955
                     /locus_tag="VWX16_001501"
     CDS             8428..8955
                     /locus_tag="VWX16_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9158830.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9032..9388
                     /gene="glnR"
                     /locus_tag="VWX16_001502"
     CDS             9032..9388
                     /gene="glnR"
                     /locus_tag="VWX16_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9158831.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9425..10771
                     /gene="glnA"
                     /locus_tag="VWX16_001503"
     CDS             9425..10771
                     /gene="glnA"
                     /locus_tag="VWX16_001503"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9158832.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLHGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10861..10980)
                     /locus_tag="VWX16_001504"
     CDS             complement(10861..10980)
                     /locus_tag="VWX16_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9158833.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11468..11663
                     /locus_tag="VWX16_001505"
                     /pseudo
     CDS             11468..11663
                     /locus_tag="VWX16_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3734 a         2135 c         2529 g         3265 t
ORIGIN      
        1 caatgtttta gaaatagagg tgtactattc tagtttcaat atactatata tctgcagaat
       61 actctttcag tttcattcag cctagtcttg cttaggaaaa agtaagaagc catccaatta
      121 tgatggatag cttcttggtt attagatttg ttctgcaaaa accttttcag caaggttcat
      181 agcgtggtct gatacacgag tgtagtgtga gatgatgtcg ataaagttga ccccagcttg
      241 tgttgaacat tcgcctttgt tgaggcgttt gatgtgggtt tttctgagaa cacgttctat
      301 cttattgatt gcttcatgac gttctacaag actgcgtgct ttttcaatat cattgttttc
      361 cacactatcc agagcatctt tgataaagtc actagtttgg cggtaaactt cctctaattc
      421 tttcaaggcg gcatcagaaa attcaacatt tttccgttga agatagtcag tcagattgag
      481 tagagcctcc gtgtggtctc caatccgttc caaatcacgg gaggaatcaa ggatattggt
      541 gagcacttca ctttcttttt ggctgagagc ttcgcttgaa agggcaatga gataacgtgt
      601 taattgctca tcgatggtgt taattgcttc ttcggtttta tgcccttttt cagcaacttt
      661 ttcatccaag tcaatgatgt acttatagga aaggtcaaag gctttagcag cgtagtttcc
      721 taagtgcaag agctctttct tagcatttcc tagagcgata gatggggcct gtttgatgaa
      781 atgttcatca agatataagg gttcgtattt tacaacctcg tcctctccag gaataatctt
      841 ggttacaaag taagccagag ctccgataaa tggaaattgg acaatggtgt tggtaatatt
      901 aaaggttccg tgagcaaagg cgatggtcat ttctggtgct agatttagcg tagcttcaaa
      961 ccaatgaatc aggacagtaa aaggaactag aaaaataacg cagacgactg ttccgataac
     1021 gttgaaggca acatgagctc ctgctacccg tttagctgca atattagccc ctaaagaggc
     1081 aatgatggct gtaatggttg tcccgatatt gtcaccaaat agaactggca aagctccctg
     1141 taggtcaatt agattgccgg cgtagaggtt ttgtaaaatc ccaatggtag ccgaagaagc
     1201 ttgaattagc aaggtcaagc cggtaccgac aaagacaccc aaaacaggat tcttacttag
     1261 ctcaatcata tagtccttaa agacctgtaa atccttgagt ggagccattg cgccgctcat
     1321 gagattgagg gcaaaaaaga taccaccgac accaaagagg atgcgtccga tattattgac
     1381 tgtccgattt tttgtaaaaa agagacagac ggcaccgata aagagcatag gtagggcata
     1441 gttacctaat ttaaaaccga tgagaaagga tgtgacagtt gtcccaatat tagcacccat
     1501 gacaatcccg atagcctgac gtaaggttaa gagaccggca ctgaccaggc cgactgtaat
     1561 aactgttaca ccagaactag actgaattag agcagtcatc ccaataccaa ccagaactcc
     1621 aaagaaagga ttactagtat atttgtcaat gtaaaaacga aggcgatctc cagcagcttg
     1681 ttgcaaaccg tctcccatgg tcttgatact atataagaat agccccagac cacctaaaaa
     1741 gtgaaataaa atttcctgcc aattaatgga catttctttt tcctccgaaa aataatcgcg
     1801 gaatatctcc tattctattt taaaggataa aagtaaatct aacaagtgtt aatgtcaagt
     1861 ttgtaaaaga aaattataat tgataagtgt attattttta agagttttta accttatttt
     1921 agatttgtga aaacagctat tgttttttgt ctaaaaatgt tttaggataa aaatgtgatt
     1981 atcatggttc tagagtttga taaaaaacta cagaatataa agcgctttct tgaaaacaac
     2041 aaaatcaatc ttttaggagg agaaaatgaa gaatccattt tttgaaagac gttgtcgtta
     2101 cagtattcgt aagttatcag taggagcctg ctcgctgatg attggtgctg ttttatttgt
     2161 tggtccagcc ttggctgaag aaactgcagt tcctgaaaat agcggagcta atacagagct
     2221 tgtttcagga gagagtgagc attcgatcaa tgaagctgat aagcagaatg aaggggaaca
     2281 tactagagaa aacaagctag aaaaggcaga aggagtagcg acagcatctg aaactgcaga
     2341 agcagctagc gcagctaaac cagaggaaaa agcaagtgag gtggttgcag aaacaccatc
     2401 tgcagaagca aaacctaagt ctgacaagga aacagaagca aagcccgaag caactaacca
     2461 aggggatgag tctaagccag cagccgaagc taataagact gaaaaagaag tccagccaga
     2521 tgtccctaaa aatacagaaa aaacattaaa accaaaggaa atcaaattta attcttggga
     2581 agaattgtta aaatgggaac caggtgctcg tgaagatgat gctattaacc gcggatctgt
     2641 tgtcctcgct tcacgtcgga caggtcattt agtcaatgaa aaagctagca aggaagcaaa
     2701 agttcaagcc ttatcaaaca ccaattctaa agcaaaagac catgcttctg ttggtggaga
     2761 agagttcaag gcctatgctt ttgactattg gcaatatcta gattcaatgg tcttctggga
     2821 aggtctcgta ccaactcctg acgttattga tgcaggtcac cgtaacgggg ttcctgtata
     2881 cggtacactc ttcttcaact ggtctaatag tattgcagat caagaaagat ttgctgaagc
     2941 tttgaagcaa gacgcagatg gtagcttccc aattgcccgt aaattggtag acatggccaa
     3001 gtattatggc tatgatggct atttcatcaa ccaagaaaca actggagatt tggttaaacc
     3061 tcttggagaa aagatgcgcc agtttatgct ctatagcaag gcctatgctg ctaaggtaaa
     3121 ccatccaatc aagtattctt ggtacgatgc catgacctat aactatggac gttaccatca
     3181 agatggtttg ggagaataca actaccaatt catgcaacca gaaggagata aggttccggc
     3241 agataacttc tttgctaact ttaactggga taaggctaaa aatgattaca ctattgcaac
     3301 tgccaactgg attggtcgta atccttatga tgtatttgca ggtttggaat tgcaacaggg
     3361 tggttcctac aagacaaagg ttaagtggaa tgacatttta gacgaaaatg ggaaattgcg
     3421 cctttctctt ggtttatttg ccccagatac cattacaagt ttaggaaaaa ctggtgaaga
     3481 ttatcataaa aatgaagata tcttctttac aggttatcaa ggagacccca ctggccaaaa
     3541 accaggtgac aaagattggt atggtattgc taacttagtt gcggaccgta cgccagcggt
     3601 aggtaatact tttactactt cttttaatac aggtcatggt aaaaaatggt tcgtagatgg
     3661 taaggtttct aaggattctg agtggaatta tcgttcagta tcaggtgttc ttccaacatg
     3721 gcgctggtgg cagacttcaa caggggaaaa acttcgtgca gaatatgatt ttacagatgc
     3781 ctataatggc ggaaattccc ttaaattctc tggtgatgta gccggtaaga cagatcagga
     3841 tgtgagactt tattctacta agttagaagt aactgagaag accaaacttc gtgttgccca
     3901 caagggagga aaaggttcta aagtttatat ggcattctct acaactccag actacaaatt
     3961 cgatgatgca gatgcatgga aagagctaac cctttctgac aactggacaa atgaagaatt
     4021 tgatcttagc tcactagcgg gtaaaaccat ctatgcagtc aaactatttt tcgagcatga
     4081 aggtgctgta aaagattatc agtttaacct aggacaatta actatctcgg acaatcacca
     4141 agagccacaa tcgccgacaa gcttttctgt agtgaaacaa tctcttaaaa atgcccaaga
     4201 agcggaagca gttgtgcaat ttaaaggcaa caaggatgca gatttctatg aagtttatga
     4261 aaaagatgga gacagctgga aattactaac tggctcatct tctacaacta tttatctacc
     4321 aaaagttagc cgctcagcaa gtgctcaggg tacaactcaa gaactgaagg ttgtagcagt
     4381 cggtaaaaat ggagttcgtt cagaagctgc aaccacaacc tttgattggg gtatgactgt
     4441 aaaagatacc agcctaccaa aaccactagc tgaaaatatc gttccaggtg caacagttat
     4501 tgatagtact ttccctaaga ctgaaggtgg agaaggtatt gaaggtatgt tgaacggtac
     4561 cattactagc ttgtcagata aatggtcttc agctcagttg agtggtagtg tggatattcg
     4621 tttgaccaag ccacgtaccg ttgttagatg ggtcatggat catgcaggag ctggtggtga
     4681 gtctgttaac gatggcttga tgaacactaa agactttgac ctttattata aagatgcaga
     4741 tggtgagtgg aagctagcta aggaagtccg tggtaacaaa gcacacgtga cagatatcac
     4801 tcttgataaa ccaatcactg ctcaagactg gcgcttgaat gttgtcactt ctgacaatgg
     4861 aactccatgg aaggctattc gtatctataa ctggaaaatg tatgaaaagc ttgatactga
     4921 gagtgtcaat attccgatgg ccaaggctgc agcccgttct ctaggcaata acaaggtaca
     4981 agttggcttt gcagatgtac cggctggagc aactattacc gtttatgata atccaaattc
     5041 tcaaactccg ctcgcaacct tgaagagcga agttggagga gacctagcaa gtgcaccatt
     5101 ggatttgaca aatcaatctg gtcttcttta ttatcgtacc cagttgccag gcaaggaaat
     5161 tagtaatgtc ctagcagttt ccgttccaaa agatgacaga agaatcaagt cagtcagcct
     5221 agaaacagga cctaagaaaa caagctacgc cgaaggggag gatttggacc ttagaggtgg
     5281 tgttcttcga gttcagtatg aaggaggaac tgaggacgaa ctcattcgcc taactcacgc
     5341 aggtgtatca gtatcaggtt ttgatacgca tcataaggga gaacagaatc ttactctcca
     5401 atatttggga caaccagtaa atgctaattt gtcagtgact gtcactggcc aagatgaagc
     5461 aagtccgaaa actattttgg gaattgaagt aagtcaggaa ccgaaaaaag attacctagt
     5521 tggtgatagc ttagacttgt ctgaaggacg ctttgcagtg gcttatagca atgacaccat
     5581 ggaagaacat tcctttactg atgagggagt tgaaatttct ggttacgatg ctcaaaagac
     5641 tggtcgtcaa accttgacgc ttcgttacca aggtcatgaa gtcaactttg atgttttggt
     5701 atctccaaaa gcagcattga acgatgagta cctcaaacaa aaattagcag aagttgaagc
     5761 tgctaagaac aaggtggtct ataactttgc ttcaccagaa gtaaaagaag ccttcttgaa
     5821 agcaattgaa gcggccgaac aagtgttgaa agaccatgaa actagcaccc aagatcaagt
     5881 caatgaccga cttaataaat tgacagaagc tcataaagct ctgaatggtc aagagaaatt
     5941 tacggaagaa aagacagagc ttgatcgctt aacaggtgag gctcaagaac tcttggctgc
     6001 caaaccaaac catccttcag gttctgccct agctccgctt cttgagaaaa acaaggcctt
     6061 ggttgaaaaa gtagatttga gtccagaaga gcttgcaaca gcgaaacaga gtctaaaaga
     6121 tctggttgct ttattgaaag aagacaagtc agcagtcttt tctgatagta aaacaggtgt
     6181 tgaagtacac ttctcaaata aagagaagac tgtcatcaag ggtttgaaag tagagcgtgt
     6241 tcaagcaagt gctgaagaga agaaatactt tgctggagaa gatgctcatg tctttgaaat
     6301 agaaggtttg gatgaaaaag gtcaagatgt tgatctctct tatgcttcta ttgtgaaaat
     6361 cccaattgaa aaagataaga aagttaagaa agtatttttc ttacctgaag gcaaagaggc
     6421 agtagaattg gcttttgaac aaacggatag tcatgttatc tttacagcac ctcactttac
     6481 tcattatgcc tttgtttatg aatctgctga aaaaccacaa cctgctaaac cagcaccaca
     6541 aaacaaagtc cttccaaaac ctacttatca accggcttct gatcaacaaa aggctcctaa
     6601 attggaagtt caagaggaaa aggttgcctt tcatcgtcaa gagcatgaaa atgctgagat
     6661 gctagttggg gaacaacgag tcattataca gggacgagat ggactgttaa gacatgtctt
     6721 tgaagttgat gaaaacggtc agcgtcgtct tcgttcaaca gaagtcatcc aagaagcgat
     6781 tccagaaatt gttgaaattg gaacaaaagt aaaaacagta ccagcagtag tagctacaca
     6841 ggaaaaacca gctcaaaata cagcagttaa atcagaagaa gcaagcaaac aattgccaaa
     6901 tacaggaaca gctgatgcta atgaagccct aatagcaggc ttagccagcc tcggtcttgc
     6961 tagtttagcc ttgaccttga gacggaaaag agaagataaa gattaaatat cgaaaaatct
     7021 tgtgaaatct ttccttatat ttccaaagtg tgatataata gtttcgaata aaataaataa
     7081 aggagttttt gtaacatggc aaaacttact gttaaagacg ttgacttgaa aggtaaaaaa
     7141 gtcctcgttc gtgttgactt caacgtacca ttgaaagatg gcgtaatcac taacgataac
     7201 cgtatcacag cagctcttcc aactattaag tacatcatcg aacaaggtgg acgtgcaatt
     7261 cttttctctc accttggacg tgtgaaagaa gaagctgata aagctggtaa atcacttgct
     7321 cctgtagcag cagacttggc agcaaaactt ggtcaagatg ttgttttccc aggtgtcact
     7381 cgtggtgctg aattggaagc ggcaatcaac gctcttgaag atggacaagt tctcttggtt
     7441 gaaaacactc gttacgaaga tgttgacggc aagaaagaat ctaaaaacga tcctgaactt
     7501 ggtaaatact gggcatcact tggagatggt atcttcgtaa acgatgcatt cggtacagct
     7561 caccgtgcac acgcatctaa cgttggtatc tcagcaaacg ttgaaaaagc agttgctggt
     7621 ttccttcttg aaaacgaaat tgcctacatc caagaagcag ttgaaactcc agaacgtcca
     7681 ttcgtggcta tccttggtgg ttcaaaagtt tcagacaaga tcggtgttat cgaaaacttg
     7741 cttgaaaaag ctgataaagt ccttatcggt ggtgggatga cttacacatt ctacaaagca
     7801 caaggtatcg aaatcggtaa ctcacttgta gaagaagaca aattggatgt tgcgaaagct
     7861 cttcttgaaa aagcaaatgg taaattgatc ttgccagttg actcaaaaga agctaacgca
     7921 tttgctggtt acactgaagt gcgtgacact gaaggtgaag cagtttctga aggcttcctt
     7981 ggtcttgaca tcggtccaaa atctatcgcc aaatttgacg aagctttgac tggtgccaaa
     8041 acagttgtat ggaacggacc tatgggtgta tttgaaaacc cagatttcca atctggtaca
     8101 atcggtgtga tggacgctat cgtgaaacaa ccaggagtta aatcaatcat cggtggtggt
     8161 gactcagctg ccgcagcgat taaccttggc cgtgcagaca agttctcatg gattagtacg
     8221 ggtggtggag catcaatgga acttcttgaa ggtaaggttc ttccaggact tgcagccctg
     8281 acagaaaaat aagattttat aaacaaatca aagaagagag ggatgaaagt tcctcttttc
     8341 ttttgcttaa aataaaaacg cttcctctca actattactc ataaaaatca ccgatttatg
     8401 ataaaatgga aatagaaagt tgagattatg agttatttta aaaaatataa attcgataaa
     8461 tcccagttca aacttggtat gcgaaccttt aaaacaggta ttgctgtttt tctagttctc
     8521 ttgatttttg gcttttttgg ctggaaaggt cttcaaattg gtgctttgac agccgttttt
     8581 agcctgaggg agagttttga tgagagtgtt cattttggga cttcgcgtat tctaggaaat
     8641 agtatcggtg gactctatgc cttggtcttt ttcttattaa ataccttttt ccacgaagcc
     8701 ttttgggtga ccttggtagt tgttccaatc tgcaccatgt taaccattat gacaaatgta
     8761 gccatgaata acaaagcagg ggttattggt ggtgtagcag ctatgttaat cattacccta
     8821 tcaattccaa gtggtgagac aattttgtac gtgtttgtgc gtgtattaga aacgtttatg
     8881 ggagtttttg tcgcaattat cgtaaattac gatattgatc gtattcgtct ctttttagag
     8941 aaaaaagaaa aataatgtta cattttataa cattatcaat tgacgtttgt ctttttttag
     9001 actataatag acagaaagaa ggaaattgta aatgaaggaa aaagaatttc gccgaaatat
     9061 ggctgttttt cctatcggca gtgttatgaa gttgaccgat ctatcggcgc gtcagattcg
     9121 ttattatgaa gatcaagagt tgatcaagcc cgatcgaaac gaaggaaatc gtcgcatgta
     9181 ttccttgaat gacatggatc gtctgcttga aatcaaagat tatatctctg aaggttataa
     9241 tatcgctgcc attaagaaaa aatatgctga acgtgaagcg aaatccaaga aagcggttag
     9301 tcagactgaa gtacgtcgtg cacttcacaa tgaactcctc caacaggggc gttttgcttc
     9361 agtacagtca ccttttggtc gcggttaggc aaccgcaagt agtcatagat taaggagaaa
     9421 acttatgcca atcacagctg cagatattcg tcgtgaagtc aaggaaaaaa atgttacctt
     9481 tattcgtctt atgttctcag atattttggg aaccatgaaa aacgtcgaaa ttcctgctac
     9541 agatgaacag ttagataagg tcttgtcgaa caaggttatg tttgatggat cttctattga
     9601 aggttttgta cgtatcaatg agtcggatat gtacttgtac ccggacttgg atacatggac
     9661 agtcttccct tggggagatg aaaatggaag tgttgcaggt ctgatctgtg atgtctatac
     9721 aacagaaggt gaaccatttg cgggtgaccc tcgtggtaat ttgaaacgag ctcttcgtca
     9781 catggaagaa gttggattca aatccttcaa tcttggtcca gagccagaat tcttcctatt
     9841 taagttggat gaaaatgggg acccaacact tgaagtgaat gacaagggtg gctactttga
     9901 tttggcacct actgaccttg cggacaacac acgtcgtgag attgtgaatg tcttgaccaa
     9961 aatgggattt gaagtagaag cgagtcacca cgaggttgcg gttggacagc atgagattga
    10021 ctttaagtac gatgaagttc tccgtgcttg tgataagatt caaatcttta aacttgttgt
    10081 taaaaccatt gctcgcaaac acggacttta cgcaacattt atggcgaagc caaaatttgg
    10141 tattgctgga tcaggtatgc actgtaatat gtccttgttt gatgcagaag gaaataacgc
    10201 cttctttgat ccaaatgatc caaaaggaat gcagttgtca gaaacagctt accatttcct
    10261 aggcggtttg atcaagcatg cttacaacta tactgccatc atgaacccaa cagttaactc
    10321 atacaaacgt ttggttccag gttatgaagc gcctgtttac attgcttggg ctggtcgtaa
    10381 ccgttcgcca cttgtgcgcg tacctgcttc acgtggtatg ggaactcgtc ttgagttgcg
    10441 ttcagtggat ccaatggcga acccttacat tgctatggct gttcttttgg aagttggttt
    10501 gcatggtatt gaaaataaaa tcgaagcacc agctcctatc gaagaaaata tctacatcat
    10561 gacagcagaa gagcgcaagg aagctggtat tacagacctt ccatcaactc ttcacaacgc
    10621 tttgaaagct ttgacagaag atgaagtggt taaagctgct ctcggagatc acatctacac
    10681 tagcttcctt gaagccaaac gaatcgaatg ggcaagttat gcaaccttcg tttcacaatg
    10741 ggaaattgat aattatttag acctttacta atatagacga aagattgcct gtgggtaaac
    10801 agtcacacag cagtctaagc catcaaatac ctataaccct taaaatagcg atttcggtcg
    10861 ctatttattt ttcgaaaaat cctcttgact cttttgcgaa ttagatttat catcagacgt
    10921 gtagaagaaa atcaaggagt cactatggaa aaattcaatt tcaaaaacaa tatcgggcaa
    10981 gaaaacaagt tacttcaaat cgaaatctac aagtttacta acttttgcaa gcttcaaaat
    11041 tacacatctg taaatatttt ttcgaaagat atatttgaag ccatagtaaa ttgagactag
    11101 aatagtacac tacatctgct aaaaatttct agaaactaat ttaactttcc taatcgattt
    11161 gtttacatct tatttcaatc tactatatta tctcaataaa tgatgatggc gactgttttg
    11221 tttgtgcttg tcgatttttg aatcctgatc gagcggaaga atatttctgc cgaggttgcc
    11281 aaggggaaaa tcctgaagat attgaatttt atgatgaaca attacaggca gaaaaggtgg
    11341 aggttttgaa tattcgattg gaagtaaaaa attgaattat ctacaatctc atcaacaata
    11401 aacctatttc aaccccatta tctataggcg tatttttacg cctttttata tacttgaagc
    11461 acataagaaa cattatggga agtttgctga tggaagcact caagaaattg atgttcctta
    11521 tgatattcct gatacttggg agtgggtgag gataaaatca atttattgga attttgggca
    11581 aaataagcca gagaaatcct ttaggtatat agatacgtct agtattgata gaaaaaagaa
    11641 cataatcaac tacaaaaatc tac
//
LOCUS       DAVKAO010000051        11592 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKAO010000051 DAVKAO010000000
VERSION     DAVKAO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.051"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(56..286)
                     /locus_tag="VWX16_001506"
     CDS             complement(56..286)
                     /locus_tag="VWX16_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9158834.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VWX16_001507"
     CDS             complement(454..1098)
                     /gene="pcp"
                     /locus_tag="VWX16_001507"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9158835.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1113..2036)
                     /locus_tag="VWX16_001508"
     CDS             complement(1113..2036)
                     /locus_tag="VWX16_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9158836.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(2033..2722)
                     /locus_tag="VWX16_001509"
     CDS             complement(2033..2722)
                     /locus_tag="VWX16_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9158837.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(2787..3491)
                     /locus_tag="VWX16_001510"
     CDS             complement(2787..3491)
                     /locus_tag="VWX16_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9158838.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(3547..4569)
                     /locus_tag="VWX16_001511"
     CDS             complement(3547..4569)
                     /locus_tag="VWX16_001511"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9158839.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(4699..7170)
                     /gene="parC"
                     /locus_tag="VWX16_001512"
     CDS             complement(4699..7170)
                     /gene="parC"
                     /locus_tag="VWX16_001512"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9158840.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLV
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(7590..9533)
                     /gene="parE"
                     /locus_tag="VWX16_001513"
     CDS             complement(7590..9533)
                     /gene="parE"
                     /locus_tag="VWX16_001513"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9158841.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            9669..10310
                     /gene="plsY"
                     /locus_tag="VWX16_001514"
     CDS             9669..10310
                     /gene="plsY"
                     /locus_tag="VWX16_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9158842.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(10391..>11592)
                     /locus_tag="VWX16_001515"
     CDS             complement(10391..>11592)
                     /locus_tag="VWX16_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9158843.1"
                     /translation="LDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCPECGNQLKKYD
                     FQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQ
                     KLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSFIA
                     QDFNNLNIITVLEGRTQAIIRNHFLRYDRVVRCRVKIITMDMFSPYYGLAKQLRFHIV
                     QHLSRAMSRVRVQIMNQFHRKFHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRIHLTN
                     KEILDKLLSYSQDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTF
                     LKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSRA"
BASE COUNT         3350 a         2540 c         2100 g         3602 t
ORIGIN      
        1 ctctcctaca atcaacatgg actattatat catgaaaatt tcaaaaaaac aagacttatt
       61 ttgctaaatt ataattttca atcaaaattt ccacagcttt ttccaatttt ttataaaaac
      121 tatcgacatt cccattcttt ataatggcaa attgtccgtc taattcggca atttctccga
      181 taactttttt accgatagtc aatgtataac cttcaaaact gtctttacca acattaactt
      241 tggcatccgc tacttgaatt tcgatttttt tatctttctt gctcatttca tacctctctt
      301 cactttttct attttacaag aaaatcctaa aactagcaag aaaaaactct atatcaggct
      361 tctctgatat agagttttat gtaaacaacc tcaattgatt tagcatagtt aatagttagt
      421 aaacaagcat aattgactat cctttttagg ttatcagtga gtctcaccgc ctactaactg
      481 caaatcctta tctttgtagt ctacaatagc ttcaatagct acctctatgc ctcttacaat
      541 atcacataga ctcattcctg cagtattcgg cttatttact acttgttcca tcatatatgg
      601 aatatgcatg aaacctgctc tcatattagg gaatttttta tccactaaat aaagagcttg
      661 gtacatcaaa tgattgcaaa caaaggttcc tgcactattg gatacaactg ccggaagtcc
      721 ctgttttttg atagcttgta ccatcgcttt gataggtaaa ctactaaaat aggccgatgc
      781 tccatcaata cgaatcggtg tatcaattgg ttgattgcct tcgttatcag gtatgcgagc
      841 atcatcttga ttaatagcca ctcgttcagg tgttaagccg gtcctgccgc ctgcttgtcc
      901 aatacaaagt acagcatctg gttgatatcg taatatttct gcctctaaaa cttctgacga
      961 cttataaaaa accgttggaa tttctaccca gcgaacttca gccccattaa tctcagatgg
     1021 taataatttt acagcctcca aagctggatt aatcttttca cctccaaaag gattaaaacc
     1081 tgtaaccaat attttcattt tattttcctt tactaaaatg cgagaaagta cattaagaat
     1141 atgtgaataa caatcattac tagagcaaca cctgcttgag cctttataac gccattctga
     1201 tctttcatat ccatcaatgc tgctggtaga gcgttaaaat tagcagccat tggggtcaat
     1261 aaggtcccac aataacctgc tgtcatggca agagcaccag ccacaattgg attagctccc
     1321 agagcaaata caaagggaac tccaacacct gctgtaataa cggtgaatgc tgcaaaagca
     1381 tttcccataa tcattgtgaa tagaaccatt ccaagaacat aagccaaaac tcctataaag
     1441 cgactatctg aaggaacaat accgctaatc agatgagaga taacatcacc aacacctgct
     1501 acagtaaaaa tagcccccaa agcccctaat aattgaggaa caatcccact tgttgaaact
     1561 tgctgagtca ttcgattatt ttctgataac agactcttag ggtgactatt ggtaatcaca
     1621 agaacagaaa ttgtagcaaa caaggcggca aggctaatcg aaatcttgct aaattctgga
     1681 atcatttgcg ctaagaccaa cgcaagtatt gccatcagca taactggaat aaaaatttta
     1741 tttttcaacc tgttagattc aatattggct ttcatttcat ctaaggatgg caaggttccg
     1801 atacggactt gcttaaacaa tgttaacagc gataatagga ttacaataat accaatactc
     1861 atatttggca tataggaacc acctataaac gtaatagaca atagagtcca aaatgcagat
     1921 gtcccaagtc gaactgggtt tgttttatct ttataactac aataggctgt atggagaaat
     1981 tgacaaccaa tcacaatata ggtcaactct aatagttgct ttgccaactc tgtcattttt
     2041 gttctcctcc cctagtcttt tttgatatca attttttatc aaataaataa ttgtaaatcc
     2101 ccactacaat aagtgttata acagcaacaa taatagatgt agaagcaatc cctgcataat
     2161 tgctttcata gcctaactga tctaatgttc cccctatcaa gaggactccc ccagcaccta
     2221 caaacgtatt ttgagcaaag aaatttccaa aattttcatt cgcagccgca cgcgctttta
     2281 ttgtctcatc ttcaacctct gttaactttc tacctaattg agactctgca gctgcttctc
     2341 ccataggttg aaccaaaggt ctgacaaact gagggtgtcc tcctagacga attgaaaaga
     2401 aaccagctaa ctctcgaata aagaaataaa ctgtatagaa gtttccaact gtcagacctt
     2461 taatctttcg aatcaaatcg attgatcgtt gcttgagtcc aaaggtttct gacagcccca
     2521 caagaggcaa ggtaaccata aaaatcgtga gcactcgctg attgctaaat tcttttccca
     2581 aaatctccaa aaattcaacg agagaaacac ctgaaactaa agctgtaacc aaaccagcta
     2641 agactactgt tgcaattgta tcaaatttta aaataaaacc cacaacaatg attgctattc
     2701 ctattaatct aatccactcc atatcaaact cctttatatt caaaatgaca gtattttaaa
     2761 aattttatca agatcaatac cattccttat ttaatgtatt tttctagttc tttttggtat
     2821 ttgctattgg attccaattt ttctttttgc cattttttaa aaacctcgtt atattctttt
     2881 gttgtaacaa tatctttttg caatttcatt cctttaaaga tatatggttc ccccttaata
     2941 ccaacttgtg agtatggttt tgagaatggt actacgttac ttacaactgg agaaccacca
     3001 gatgaagctg ttggcatcaa taatgaacta tctgtcgacc aagcttgagc tttggcatat
     3061 ttttcatatc tcttctctag gtcagtggtc tcagaaacag catcttctaa caatttctta
     3121 tatttatcca aaccaggttt agctacaaca tccttatctt ttcctttcgt aatgccaagg
     3181 tgtttcatgg cagaaccaga ttttggatcc ataatattca agtaagacgc tggatcttga
     3241 tagcttggag cccatcctgt actgttcaaa tcatagtctt tttgagaagg agcaacattg
     3301 ccgtatttat cattttccat caaaccatca ataacatttc caataacgtc tgtcctcgat
     3361 gttcgagtcg ctatactgta gcccaatgat gctggatcta ctgcatagac ataagaaaat
     3421 gttgtcggtg catctgcttc tttatcagtt tttccacaag ccactaaaat agctgacgtg
     3481 ctcaggacca ctcctgctgt taagagccac tttttctatt tcataaaaaa tctcctttgg
     3541 tttattttaa tctactttta caatccaacc ttctggcgct tcaatatcgc caaactgaat
     3601 acccgtcaat tcattatata atttacgcgt cacaggacct acttctgttt cactatagaa
     3661 tacatggaaa tcatcaccat gttgaatacc tccaattgga gaaataaccg ctgctgtacc
     3721 acaggcacct gcctctacaa aacggtcaag attatcaatt ggaacatcac cctcaatagg
     3781 agttaatccc aagcgatgtt ctgccaaata aagcaaggaa tacttggtaa tagatggcaa
     3841 gatagatgga ctcaatggtg ttacaaattc attatcagct gtaattccaa agaaattagc
     3901 tgatccgact tcttcaatct ttgtatgagt tgatgggtcc agatagataa catctgagaa
     3961 atggcgtgac ttggccattt ttcctggtaa gagacttgca gcatagtttc caccaacctt
     4021 agccgcacct gtaccatttg gtgctgcacg gtcgtactca tcctgaatca agaagttggt
     4081 tgggaccaaa ccacctttaa agtaatttcc aactggcata gcaaagatgg tgaaaatgta
     4141 ctcttctgcc ggttttaccc cgataatatc tccgacacca atcaaaagag ggcgaagata
     4201 taaagttcca cctattccgt atggtggtac gtattcttca ttcgcacgga caactgcttt
     4261 acaagcttct acaaacatgt ctgtcggaac ttgtggcatc aagagacggt cacatgtacg
     4321 ttgcaggcgt ttagcatttt catcaggacg gaacagttga acactgccat ccttagtacg
     4381 ataagctttc aaaccttcaa atgcttgttg tccatagtga agacttggag aagactctga
     4441 aatatgcaaa gttgcatcct ctgtaagctc tccttgatcc cattgtccat ttttgaaatg
     4501 agcaagatag cgataaggta atttcatata ggaaaaaccg aggttttccc aatcaatcgt
     4561 tactgtcata ttccactcct tattctaaaa acctatttct tatattctac actatttttc
     4621 taaaatagca agtatatttt gtaattttca gaaaatttct ccaataaaaa ccatcttaga
     4681 actgattctt catttcactt atttatcttc agtaactact tcctgaagat aagcgtcaaa
     4741 aacttcttca tctgaaatcg tgtcagaaat gaagcttcca ttgctagtgc gttctgacaa
     4801 gttcaagtct tgcaatcggc tttcatagat tgttccttta ttggattgga caagcagagt
     4861 ttggtcgttc acatccactt ccgtactgaa gaaatcgcca acaaatcctt gctctgcaac
     4921 tgctcctgcc aagaagacac gatgcggttt gtttttcaac tcacgcaaga cttgtaatcc
     4981 tcgtttggca cggctggttg ctagaatttc ctcaatggaa acacgtttca agcttccacg
     5041 ctgggtcaag aggtagaagg acgaagtatt acagataaag ccagattgga ggacatcatc
     5101 ttctttcaaa ttcatagcct tgacacctgc tgccttagca ccgacaaccg gaacctcttc
     5161 gatattgaaa cgcagggcat aaccattttg actaaccaag acaacatcat ctagtttaat
     5221 cggagccact gctacaatct gatctgtatc gtctttgagc ttagcatact tgacagactt
     5281 agatctatag gtccgccatg gagtgaattc ttttcgctct acccgtttga tttgaccaag
     5341 gcgagtcgct gcaaagtagg ttgtcgcatc gtcaaactga tccagtactt ccacataaag
     5401 gattgcttca ttcgtttcaa agtttgtgat ggtttggctc agatgctctc cgatgtcctt
     5461 ccaacgaata tctgccaact catggattgg tctgtagatg acatttccaa gacttgtgaa
     5521 catcaagagg tgctgggttg tcttggcaga ttgaacaaaa atcaaacggt catcatcacg
     5581 cttgccaatt tcttccaagg tggaagccgc aaaggaacgt ggactggtac gcttgatgta
     5641 acctgccttg gtcacgctga cgtaggtatc ttcctcagcg ataagactag ctgtatcaat
     5701 ctcaattgct ttcgcagtgt cttctaaaga actcaaacga ggagttgcaa atttcttctt
     5761 gacctcacga agttctttct tcatgagatt gtacatagtc ctttcatcac cgataatagc
     5821 caccagcata gcaatcttct cacgaagctc tgcttcttct tcctgcaaga caaccacatc
     5881 ggtattggtc aaacggtaca gttgcaaagt tacgatagcc tcagcctgtt cttccgtaaa
     5941 atcatagcta actttgaggt tttccttggc gtccgcctta ttctcagaag cacggataag
     6001 agcaatgact tcatccaaaa tcgaaatcac acgaatcaaa ccttcgacga tatggagacg
     6061 tttctcagcc ttttctttgt caaagcgtga acgcgccaaa atcacttctc gacggtgagc
     6121 gatgtagcta gacaggattg gaacaatccc aacctgacga ggtgtgaaat tgtcaatcgc
     6181 caccatatta aagttgtagt tgatttgtag gtcggtgtac ttaaataagt agttgagaac
     6241 aagctcagta ttagcgtctt tcttaagttc gatagcgata cgaagaccat cacggtcaga
     6301 ctcatcacga acctcagcaa tcccagctac cttgttatta acacgaacat catcgatttt
     6361 cttgactaga ttggccttat tgatttcata aggaatctca gtaataacga tttgttcctt
     6421 accacctttt agcttttcaa tttcagtctt ggaacgaaca accacgcgcc ctttcccagt
     6481 ctcataagct ttcttgattt catcacgacc ctgaataata gcccctgtag ggaagtctgg
     6541 tccaggcaag aattccatga gtttatcaat ctttgcagtt gggtggtcaa tcatgtaaac
     6601 tgcagcatct atgacctcag ctaaattatg gggaggaatg tctgtggcat aaccagccga
     6661 aatcccagtc gaaccattga ccaagaggtt tggaaaggct gctggcaaga ccgttggttc
     6721 tttctccgta tcgtcaaagt tccatgcaaa aggaactgtc tttttctcga tatcctgaag
     6781 aaggtagcct gcaatttcag acaaacgtgc ctcagtataa cgcatagccg caggaggatc
     6841 tccgtccata gaaccgttat taccgtgcat ttcaactaga atctcacgat ttttccagtt
     6901 ctgtgacata cgaaccatgg catcatagat agaagaatcc ccgtgtgggt ggaaattccc
     6961 catgatgttc ccgactgact tggccgactt acggtagctc ttgtcaaaag tattgctatc
     7021 cttattcata gaataaagaa tacggcgctg aaccggcttc aacccatcac gaatatctgg
     7081 caaagcccgg tcttgaataa tgtacttgga gtagcgacca aagcgctctc ccatgatgtc
     7141 ctccagggac atgttttgaa tgttagacat aagatacaaa gcccataaaa taccaagtga
     7201 aaatagaaaa ttcttgaagt aagcaaactc acaagagaat ttatcttttt cacacagtat
     7261 ctagggcgtg ttcaactcct ttcaaagaat gtagagtagg tttttatgca gtaaaagata
     7321 ttttacggga attcgtcccg tgttcagtta cgataagtaa ccaaactatc ctgtttgtat
     7381 ttttcaatat gaaaatatgg ttttccaaaa ttagtcttag tttgtgtctt agccgctccc
     7441 ttaaacgcct ctttgagata agcactcata gcagattctt cattaataat cctgcaattt
     7501 tttcaaacca agattttcaa actgcttttt cacatagtca ttcacatccg actctaattt
     7561 ccagtttact aacatattat tttctttcat taaaacactg tcgcttcttc tagcgtaaac
     7621 ttgacattat cttcaatcca tttacggcgt ggttctacct tatctcccat gagaacattg
     7681 acgcggcgtt cggcgcgcgc taaatcttca attgtgacac ggatgagggt acgtgtttct
     7741 gggttcatgg ttgtttccca gagctggtcc gcattcatct caccaagtcc tttgtatcgt
     7801 tggagggtag cgcctttacc gaactgttta cggagttctt ctagttctcc gtccgtccaa
     7861 gcgtaggcca cttcttcttt cttgccttta cctttggaca tcttgtaaag aggtgggagg
     7921 gcaatataga catgacctgc ctcgactagc ggacgcatgt aacggtagaa aaatgtcaag
     7981 agcaaggtct ggatatgggc accgtcggta tccgcatcgg tcatgataat gatcttatca
     8041 tagttggcat cttcaataga gaagtctgct ccaacacccg caccaatggt ataaatcatg
     8101 gtattgatct cttcattttt gaggatatcc gccatcttgg ccttggctgt attgacaacc
     8161 ttaccacgaa gaggtagaat agcctggaac ttgcggtcac gaccttgttt ggcagaacca
     8221 ccggcagagt ccccctcaac tagatagagt tcattcttag caggattctt agattgggct
     8281 ggggtcaatt tcccagacaa caagccctta tctttcttgt ttttcttccc atttcggctc
     8341 tcatcacgcg ccttacgtgc tgcttcacga gcatcacggg ccttgatagc cttgcggatg
     8401 aggttagaag ctaattcccc attttccata aggaaaaagg tcaacttatc agccactatt
     8461 ccatccacaa ctgggcgagc tagggggctt cctagtttat ccttggtctg tccttcaaac
     8521 tgcaagtgtt cttcaggaac taagatagaa agaacggccg ctagtccctc acgatagtct
     8581 gaaccttcaa ggtttttatc tttttccttg agaagacctg ttttacgtgc atagtcattc
     8641 atgaccttgg taatggcaga cttgagtcct gtctcgtgcg ttccaccgtc cttggtgcga
     8701 acgttattga caaaggatag aatgttatct gagaatccgt cattgtactg gagggctact
     8761 tccacttgaa aaccattgtc ttccccttca aagtaaagaa ctggcgtcaa gatttcctta
     8821 tcttcgttga gataagaaac aaaatcttgt actccattct catagtggaa ctcaatcgct
     8881 tcatctgttc gcttgtccgt taaagacaag gtcacatttt tcaagagaaa ggctgattca
     8941 ttaaggcgct ctgaaatggt attgtacttg aaatctgtcg tagaaaagat agtcgcgtca
     9001 ggcataaaag taactttggt gcctgtttta gacttgggtg ctgtaccgat tttcttcaaa
     9061 gtcgtgacag gttttccacc attttcaaaa cgttgcttgt aaactgcgcc atcacgggta
     9121 atttcaactt ctaaccagct agaaagggcg ttaacaacgg aagaacccac tccgtgaagt
     9181 ccacctgatg tcttatagcc accttgaccg aatttccctc cggcatgaag aatggtaaag
     9241 ataacctcaa cagttggaat tcccatagcg tgcatacctg tcggcatccc acgtccatgg
     9301 tcttgaaccg ttagactacc gtctttattg atagttacat caatacgatc accaaaccca
     9361 gacaaggctt catcgactgc attatcaacg atttcccaaa ctaggtgatg aagaccagcg
     9421 ccatcggtcg atccaatata catccctgga cgttttcgga ccgcatccaa cccttctagc
     9481 acctgaatag catcatcatt ataattgtta atattgattt ccttttttga cacaaggaac
     9541 ctcctattcg ttcatcttta ctattctaca ggttttccaa ggattttgca aaatttttct
     9601 ttctccgatg tgacaatttc agcagagatt ctctgctttt ctttcccaat tcatgatata
     9661 ataggagtat gattacaata gttttattaa tcctagccta tctgctgggt tcgattccat
     9721 ctggtctctg gattggacaa gtattctttc aaatcaatct acgcgagcat ggttctggta
     9781 acactggaac gaccaacacc ttccgcattt taggtaagaa agctggtatg gcaacctttg
     9841 tgattgactt tttcaaagga accctagcaa cgctgcttcc gattattttt catctacaag
     9901 gcgtttctcc tctcatcttt ggacttttgg ctgttatcgg ccataccttc cctatctttg
     9961 caggatttaa aggtggtaag gctgtcgcaa ccagtgctgg agtgattttc ggatttgcgc
    10021 ctatcttctg tctctacctt gcgattatct tctttggagc tctctatctt ggcagtatga
    10081 tttcactgtc tagtgtcaca gcatcgatcg cggctgttat cggggttctg ctctttccac
    10141 tttttggttt tatcctgagt aactatgact ctctcttcat cgctattatc ttagcacttg
    10201 ctagtttgat tatcattcgt cataaggaca atatagctcg tatcaaaaat aaaactgaaa
    10261 atttggtccc ttggggattg aacctaaccc atcaagatcc taaaaaataa aacgccagtt
    10321 ctgtactgcc cccaaacagt tagacaaata atttatggct ctttgtcaac tgtagtgggt
    10381 tgaagaaaag ctaagctcga gaaaggacaa attttgtcct ttcttttttg atattcagag
    10441 cgatgaaaat ccgttttttg aagttttcaa agtttcgaaa accaaaggca ttgcgcttga
    10501 taagtttgat gagattattg gtcgcttcca gtttggcatt agaatagtgt agttgaaggg
    10561 cgttgacaat cttttcttta tctttgagga aggttttaaa gacagtctga aaaataggat
    10621 gaacctgctt aagattgtcc tcaataagcc cgaaaaattt ctctggttcc ttattctgga
    10681 agtgaaaaag caagagttga tagagctgat agtgatgttt caagtcttgt gaatagctca
    10741 aaagcttgtc taaaatctct ttattggtta aatgtatacg aaaagtagga cgataaaatc
    10801 gcttatcact cagtttacgg ctatcctgtt gtatgagctt ccagtagcgc ttgatagcct
    10861 tgtattcatg gaattttcga tgaaactgat tcatgatttg aacacgcaca cgactcatag
    10921 cacggctaag atgttgtaca atgtgaaagc gaagctgttt agccaagcca tagtaagggc
    10981 taaacatatc catagtaata attttgacgc gacatcggac aactctatcg tagcgaagaa
    11041 agtgatttcg aatgatagct tgtgttcttc cctcaagaac agtgatgata ttgagattgt
    11101 taaaatcttg cgcaatgaag ctcatctttc cctttgtaaa agcatactca tcccaagaca
    11161 taatctcagg aagacaagaa aaatcatgtt taaagtgaaa atcattgagc ttacgaataa
    11221 cagttgaagt tgagatggaa agctgatggg caatatcagt catagaaatc ttttcaatta
    11281 acttttgagc aatcttttgg ttgatgatac gagggatttg gtgattcttc ttgacgatag
    11341 aagtttcagc gaccatcatt ttagaacaat gatagcactt gaatcgacgc tttctaagga
    11401 gaattctagt aggcatacca gtcgtttcaa gataaggaat tttagaaggt ttttgaaagt
    11461 catatttctt caattggttt ccgcactcag ggcaagatgg ggcgtcgtag tccagtttgg
    11521 cgatgatttc cttgtgtgaa tccctattga cgacatctat aatttggata tttgtgtctt
    11581 tgatatcgag ta
//
LOCUS       DAVKAO010000052        11552 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKAO010000052 DAVKAO010000000
VERSION     DAVKAO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.052"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            116..874
                     /locus_tag="VWX16_001516"
     CDS             116..874
                     /locus_tag="VWX16_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9158844.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            914..1678
                     /locus_tag="VWX16_001517"
     CDS             914..1678
                     /locus_tag="VWX16_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9158845.1"
                     /translation="MISLFGLVAAKPVQANTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIHYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1691..2368
                     /locus_tag="VWX16_001518"
     CDS             1691..2368
                     /locus_tag="VWX16_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9158846.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2378..3037
                     /locus_tag="VWX16_001519"
     CDS             2378..3037
                     /locus_tag="VWX16_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9158847.1"
                     /translation="MESILEILTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3274..4398
                     /locus_tag="VWX16_001520"
     CDS             3274..4398
                     /locus_tag="VWX16_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9158848.1"
                     /translation="MEFSMKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSL
                     TFILATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNL
                     AIYIFASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFL
                     VGSYALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQF
                     AFLKIFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYG
                     HMIIEEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNY
                     AQKNPNFELHLIDSTKDGYLNFEQKKCPNMQPSICVVLFL"
     gene            complement(4796..5677)
                     /locus_tag="VWX16_001521"
     CDS             complement(4796..5677)
                     /locus_tag="VWX16_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9158849.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADSVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5818..7146)
                     /gene="vncS"
                     /locus_tag="VWX16_001522"
     CDS             complement(5818..7146)
                     /gene="vncS"
                     /locus_tag="VWX16_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9158850.1"
                     /translation="MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKNRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(7143..7799)
                     /gene="vncR"
                     /locus_tag="VWX16_001523"
     CDS             complement(7143..7799)
                     /gene="vncR"
                     /locus_tag="VWX16_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9158851.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7848..7931)
                     /gene="pep27"
                     /locus_tag="VWX16_001524"
     CDS             complement(7848..7931)
                     /gene="pep27"
                     /locus_tag="VWX16_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9158852.1"
                     /translation="MRKEFHNVLSSGQLLTYKRPARDYNRK"
     gene            complement(8112..9491)
                     /gene="vex3"
                     /locus_tag="VWX16_001525"
     CDS             complement(8112..9491)
                     /gene="vex3"
                     /locus_tag="VWX16_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9158853.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9543..10190)
                     /gene="vex2"
                     /locus_tag="VWX16_001526"
     CDS             complement(9543..10190)
                     /gene="vex2"
                     /locus_tag="VWX16_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9158854.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIQ
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10203..11480)
                     /locus_tag="VWX16_001527"
     CDS             complement(10203..11480)
                     /locus_tag="VWX16_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9158855.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQVSLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3254 a         2582 c         2182 g         3534 t
ORIGIN      
        1 gatcgatttg tcctgttctt atttcactat agtgaatttt ttcagatatg gtttgacaag
       61 aacattcttt tattgtagaa tcgtgttata aaatctaaca taaatgagtt ctctcatggc
      121 tttagtagaa tttaaaaacg tcgaaaaata ttacggagac taccacgcac tccgcaacat
      181 caatctccgt tttgaaaaag gacaagttgt tgtcctgctt ggaccttctg gctctgggaa
      241 gtccactctt atccgtacga tcaatggttt agaggctgtt gacaaaggaa gtctcctagt
      301 caatgggcac caagttgctg gtgccagcca gaaagatttg gtacctcttc gcaaggaagt
      361 cggcatggtt tttcaacatt ttaaccttta tccacacaaa acagtgttag aaaacgtgac
      421 acttgcaccc attaaagttc taggaattga taaaaaagaa gctgaaaaaa ccgcccaaaa
      481 atatctggaa tttgtaaata tgtgggacaa gaaagattcc tatcccgcca tgctatctgg
      541 tggacaaaaa cagcggatcg ccatcgctcg tggtcttgct atgcatccgg aactcctcct
      601 ctttgatgaa ccaacatctg ctcttgatcc tgagactatc ggagatgttc tagcagttat
      661 gcagaaactg gcgcgtgatg ggatgaacat gatcatcgtt acccacgaaa tgggctttgc
      721 tcgagaggtt gcggaccgca ttatctttat ggccgacgga gaagttttag tagatacgac
      781 agatgtcgat aacttttttg acaatccaag cgaacctcgt gcccaacaat tcctcagcaa
      841 aattatcaac cacgaaagtg acaaagtcaa ataaggaggc gcctatgaaa aaaaattctt
      901 tttatcagca ttattgatta gccttttcgg ccttgttgct gccaaaccag tccaggctaa
      961 tactagtatc gcagacattc aaaaaagagg cgaactggtt gtcggtgtca aacaagacgt
     1021 tcccaatttt ggttacaaag atcccaagac cggtacttat tctggtatcg aaaccgactt
     1081 ggccaaaatg gtagctgatg aactcaaggt caagattcac tatgtgccgg ttacagcaca
     1141 aacccgcggc ccccttctag acaatgaaca ggtcgatatg gatatcgcga cctttaccat
     1201 cacggacgaa cgcaaaaaac tctacaactt taccagtccc tactacacag acgcttctgg
     1261 atttttggtc aataaatctg ccaaaatcaa aaagattgag gacctaaacg gcaaaaccat
     1321 cggagtcgcc caaggttcta tcacccaacg cctgattact gaactgggta aaaagaaagg
     1381 tctgaagttt aaattcgtcg aacttggttc ctacccagaa ttgattactt cccttcacgc
     1441 tcatcgtatc gatgcctttt ccgttgaccg ctctattcta tctggctaca ctagtaaacg
     1501 cacagaacta ctagatgata gtttcaagcc atctgactac ggtattgtta ccaagaaatc
     1561 aaatacagag ctcaacgact atcttgataa cttggttact aaatggagca aggatggtag
     1621 tttgcagaaa ctttatgacc gttacaagct caaaccatct agtcataccg cagattaagg
     1681 aggataccct atgacagatt tatcatcttg gacagcctat tttcaggatt ttggacaatt
     1741 tttcaatggt ttcctcttta ctcttgccct agcgattgga tcctttatcc tcgccatggt
     1801 cttaggaatc ttctttggtg ccatgtcaac cagtaaacgt ccaattttac gcattttagc
     1861 tcgcatcttc gtggaatttt accaaaacac tcccctcttg gtgcagtttg ttattgtttt
     1921 ttatggccta cctcttatca gtgaccacat catcatgatt ccaatttatt ggacagccgt
     1981 tctctgcgtg ggactctatc acggcgctta tatcgctgag gttattcgtt cagggattca
     2041 gtctattcct agtggtcaga tggaggccgc cttgtcgcaa ggttttacct atatcagtgc
     2101 catgcgcttg attatcttgc ctcaggcctt ccgcatcatt ctccctccat tgaccaacca
     2161 aattgttaac ctcatcaaga acacatctac tgtagctatc atctctggag tagacttgat
     2221 gtttgtgact aagtcttggt cggctctcaa cggaaactat atcccagcct ttttaggtgc
     2281 tgctcttctc tactttgccc tatgcttccc tgttgcccag tttggtcgca agatggagca
     2341 agccaataaa aaagcctatt cactttagga ggttactatg gaatctattt tagaaatttt
     2401 aaccccagat aacctagtct ttatctttaa aggatttggc ttgaccctct atatttctct
     2461 gattgccatc atcctctcta ctatcatcgg taccgtgctt gccgtcatgc gaaatggaaa
     2521 aaatcctatc ttacgcatta tttccagtat ttatatcgag tttgtgcgca acgttcccaa
     2581 ccttctctgg atttttacta tctttttggt gttcaaaatg aaatccacac cagcaggtat
     2641 tacagccttt actctcttta catcagcagc cttagctgag attattcgag gcggtctcaa
     2701 tgccgtagac aagggacaat acgaagcagg aatgtcacaa ggcttcacct cagcccaaat
     2761 cctctactac atcattctcc cacaagccat ccgcaaaatg ctaccagcca tcatttctca
     2821 gtttgttacc gtgattaagg ataccagtct cctctactct gttatcgccc tacaagaact
     2881 ctttggagcc agccaaattc tcatgggccg ttatttcgaa ccagagcagg tcttcagtct
     2941 ttacatcctg attgccctca tctacttcag ctttaaccta gcgatttcta gcctgtctca
     3001 tatgctagcc aaacgttggc aacaagctgc agaataatac tcttcgaaaa tctcttcaaa
     3061 ccacgtcagc ttcaccttgc cgtactcaag tacagcctgc ggctagcttc ctagtttgct
     3121 ctttgatttt cattgagtat aaacagccac tctccagttc aattggagag ttttttgatg
     3181 agagtagata tttatagacc tcaatttgac accatacatt ttctatgata aaatgtaaca
     3241 aattcattta caacaaatcg aaatctaaat gaaatggaat tttctatgaa atcagtcaaa
     3301 ggactactct ttatcatagc tagttttatc ttgactcttt tgacttggat gaacacttct
     3361 ccccaattca tgattccagg actagcttta acaagcctat ctctgacttt tatcctagcc
     3421 actcgtctcc cactactaga aagctggttt cacggtttgg agaaggtcta caccgtccac
     3481 aaattcacag cctttctctc aatcatccta ctaatctttc ataactttag tatgggcggt
     3541 ttgtggggct ctcgcttagc tgctcagttt ggcaatcttg ccatctatat ctttgccagc
     3601 atcatccttg tcgcctattt aggcaaatac atccaatacg aagcttggcg atggattcac
     3661 cgcctggttt acctagccta tattttagga ctctttcaca tctacatgat aatgggcaat
     3721 cgtctcctta catttaatct tctaagtttt cttgttggta gctatgccct tttaggctta
     3781 ctagctggtt tttatatcat ttttctatat caaaagattt ccttccccta tctagggaaa
     3841 attacccatc tcaaacgctt aaatcacgat actagagaaa ttcaaatcca tcttagcaga
     3901 cctttcaact atcaatcagg acaatttgcc tttctaaaga ttttccaaga aggctttgaa
     3961 agtgctccgc atcccttttc tatctcagga ggtcatggtc aaactcttta ctttactgtt
     4021 aaaaattcag gcgaccatac caagaatatc tatgataatc ttcaagccgg cagcaaagta
     4081 accctagaca gagcttacgg acacatgatc atagaagaag gacgagaaaa tcaggtttgg
     4141 attgctggag gtattgggat cacccccttc atctcttaca tccgtgaaca tcctatttta
     4201 gataaacagg ttcacttcta ctatagcttc cgtggagatg aaaatgcagt ctacctagat
     4261 ttactccgta actatgctca gaaaaatcct aattttgaac tccatctaat cgacagtacg
     4321 aaagacggct atcttaattt tgaacaaaaa aagtgcccga acatgcaacc gtctatatgt
     4381 gtggtcctat ttctatgatg aaggcacttg ccaaacagat taagaaacaa aatccaaaaa
     4441 cagagcttat ttacgaagga ttcaagttca aataagtaat catactctcc tgggatttcc
     4501 caggagagtt ttttatagcc aaatgaaata agacctgaac gatggaattt tggcattcaa
     4561 atcaattgat aagaatattt tatagtggag tgaaatagga tacgaacaaa ttgattagga
     4621 aaatcaaatg aatttataga aatcttttag cagttatgtt gtcctattct agtttcgaaa
     4681 cgctatagaa gcagcattgt gctagtctag attcagttta ctatactaaa acgagtagct
     4741 tgaaatcaaa aaacccaccc tcacaggcag gttttatctg tattattcag ctagattatg
     4801 ctttaccttc tgaaccgaat acgtcgatac gttcttcaac cgatgcttgg atagctttta
     4861 cactgtcagc caagaattta cgtgggtcga agagtttttt cttgtcgtat tctgcttcgt
     4921 ttgcttcgta gtcacgagca aatttacgag ttgcgttagc gaatgcgatt tggcattctg
     4981 tgttaacgtt aactttggca acaccaagtt tgatagctgc ttggatttgc tcatcaggaa
     5041 tacctgatcc accgtgcaat acgattggga atcctggaag agcttctgtc aatttttgca
     5101 agtggtcaag gtcaagacct tcccagttta ctgggtaagg gccgtggatg ttaccgatac
     5161 cagctgccaa gaagtcgata ccagtttcaa ccattgcttt agcgtcttcg attggagcca
     5221 attcaccttt accgatgatt ccgtcttctt caccaccgat agtaccaact tcagcttcta
     5281 ctgagatacc tttagcgtgt gctttttcaa caacttcttt agccaattta aggttttctt
     5341 caactggaag gtgtgaaccg tcaaacatga ttgaagtata accaacttcg atacactcaa
     5401 gtgcatcttc gtagtgaccg tggtcaaggt ggatagctac tggtacagtg atacccattg
     5461 attcaacaag gttagcgatc aagttgcgag caactttgta accacccatg tatttagcag
     5521 cacccattga agtttggatc aaaactggag cttttttagc ttctgctgcg cgcaagatag
     5581 cttgagtcca ctcaaggttg tttgtgttaa atccaccaac tgcataaccg ttgtcacggg
     5641 ctgcttggac aaatttttct gctgaaacga ttgccatttt atcaggcctc ctgtatattt
     5701 ttatgggtca tcccatttac attgttcatt ttatcacttt ttgccaaaaa aatctagttt
     5761 ttcccgcagt ttcgattgat tttcttctaa ctccatctat gtaaaccctt tctctcccta
     5821 gtcttggacg acttttggaa aatctataaa gaaggttaaa cgattctcct ccatctcgaa
     5881 acgataagct aatttttcat gttctaatag actcttaacc acaaagagcc ccataccaga
     5941 ccccttgacc ttgcgactgg cattgtcaga aaaagactgg gctagttttt cttgttcctc
     6001 tgagctacag ctattttcga taaaaagttc tccttctctt tctccaattc gaactaagcc
     6061 acctggaaca gagtgcttaa tggcattgct gatgagatta gaaagaatca acttcataac
     6121 tgatgggttt agataagcct gctgatgggt caaactattg tctatctgga gctctctttc
     6181 cttggctagc aaggcataat ctttgaccag attttgcgtc atctggagga ggtcaattgt
     6241 ttccctatca tctcgcaatt cctgcacaga agagagggaa agtatctgca gaacatggtg
     6301 attgagttca tccacaatcc ccaaggcaac tcccagatac tggtctctat tcttataacg
     6361 accgatattc tctctcatat tttcgattag gattttcaaa ctagccagcg gtgttttcaa
     6421 ttcatgagaa gctcctcgta ggaattcgac cttcatcttc tccagctgga gaatggcttc
     6481 attcttttca tgcaagtccg caataacagt caagagatgc tggtagaggc tattgatttg
     6541 ttccttgaga ttgcctatct catccttaga atccacgcgc aatcgcactt gggaatccag
     6601 gtccatcatc cgacgggtca cccgcttgat ttccaaaatc ggtgcaacaa tagtccgagc
     6661 gtagatgtag gccaccaaga gggaaatcag aaaggaggcc agcaaggtat agggaagaaa
     6721 ttggagactg atttgctccg cttccttttg taaatccatg gaagctagaa actggagaat
     6781 catagtacca ccgtcttgcg ttttcacctc gcgctcctca ataaagagag aggttgtctg
     6841 gcggtctgtg tccagaggaa gactgtcctt gacttctaac ttgtcctcgg tcatctcacc
     6901 tttgacggtc cccttgatat cactagtctg ggaatacaag tctaacactt gctcgatact
     6961 ctgcctatct ttcccttcta gggactgggc aatggctgtt gccttttgac caatggtttc
     7021 ctgacgatga ctcagataag tcgaaggaaa aagaaaataa atagctaaat gaaggcagat
     7081 aaccagaaca ctaaatatcg agaaggtata gataaatatc tttgtaaata aacctgttcg
     7141 tttcattttc gctccaattt ataaccaaca ttgcgcacag tgaggataca atccaagtct
     7201 agctttttcc gcaattcctt gatataaaca tcaataacac ggtcaaaggg aacctcatct
     7261 gtcgctttcc agacggcatc gataatctga gaccgagtca aggcccggcc ttcatttttc
     7321 actagatagt ccagaatttc caactctttg gcattgatag gcacttcttg acctgcgagg
     7381 cttgcactgt agctttcaaa gtccaccttg gtatccttgt aagaaaagat tcgtcctgta
     7441 tcgtagtagc gcttgaaaat cgcgtccacc ctcactttta agagggagag ggagaaaggt
     7501 ttttccagat agccatctgc caaagaggca aaggcactca tcttgtattc ctcatcctga
     7561 aaagctgtca acatcaagac aggaacctga ctggttttac gaatctcagc taggacttct
     7621 aagccgttaa gcttgggcat ctggatatcc agtaaaacca gggccacctc atagctagaa
     7681 aattgctcca gagcttcctg accgtccgct gcctcaatag tttcatagcc acaatccgtc
     7741 aaataatcac tgaccccctc acggatcatc tcttcatctt ctacaattaa aattttcata
     7801 ctttaactgc tctctatttt ttatttttct tagaataaat acctactcta ttttctatta
     7861 tagtctcttg ctggcctttt gtatgtaagc aactgaccac tagataaaac gttgtgaaat
     7921 tcctttctca taaattccat aactttagta tattatattt aagcactaaa gtacaaagaa
     7981 agcaactgaa agcaatgatt ttcaccactg ctttcagatt tattttgaat tgttaaatag
     8041 ctattcctat ccactattct tgaatagaaa cacaagatgc aatctttatt ccagactcat
     8101 ttttttcaaa tttattcacc atccagcaag agctcttttg gttgttttct aaggagattg
     8161 cttgaagcaa gcgccataac gagaaccact agaaccaagg caaggacaaa aatgatgata
     8221 aagtctgatg tctgaatgga aatgtctagg ctcgacaagg tcttgctaaa gccatctact
     8281 tctgcaccgc caccaaggtt agaggcttga gccgccttac tagcctgttt ggcaacacct
     8341 gaagtcacat tggcaaggac agtgtttcca attgcacggg cagtgtaatt agctaggaag
     8401 taagcagaaa ctagagcagg gatagcaatc aagatagatt cggtgatgaa ttgacccaag
     8461 atacttgcct gcttgagacc aatagagagg aggattccca cttccttgcg acgggcattg
     8521 atccaaagac tgagcaagag ggcaaggaga aggactgaga agctcaagct accccagaag
     8581 aggaggttgg ccatcttgta cataccagag atagattgct caagagctgg gtagttagag
     8641 gagctcttga cgagtgtgta gctcttccag ttgataccac tgatgccatt caactctttc
     8701 ataacatcat ccaagttctt gtctgctgtt acaaagaagg ttgcgtcccc ataaatggct
     8761 gtgtcttctg tgtatccata aagttttgca gcagtgtgaa tgtctgtaat agctgtgttt
     8821 tcgtaaagtt cttgtgagta ggttactgct gacttattat gaccatcaaa gagtcccttg
     8881 attgtcactt caactgtttc cttggctcct ttttcattat ctgcatcgta gatattagag
     8941 tccagtttaa ccttgtcccc tactttccag ccgtgtttgg ctgccaagtc cttgtgcaag
     9001 aggattttat ccttgtcgtc gttggttaag tgctctcctt cgactagttt ataagaacca
     9061 gagacaaact tgtcttcttt agaggagtca ttgacacctg taatcatcaa gctacttcca
     9121 aaacgtttgg cacgatcagc agtgagattc ttcttggttt ctggcgtttc aatcaggtca
     9181 tatccagtca aatctccgat agcgttgata cgtttgacat aagactcaat ggccttgttt
     9241 tcggtgattt ttttgatgtc ttcacccttg atattcccag caccacgagg cgttccttgg
     9301 ttgacgcgac gattgatttg catggagaag ctattggtga tatttttaaa ggtctcctga
     9361 gaagccttgg cagtagctcc cttgattgac aagccgacca aactcaagct cgccataagg
     9421 agaataatca ggaagatgac aatcgatttg aaaaacttcc ttgtaacata ggcaaatgcg
     9481 ttgtgtaaca tagattccct ttctagattt tgttttaatc attctatcaa aataagctca
     9541 aattatttac tagtattgcg cgtttcagtc agtttcttat cctttaattc aagtgtaata
     9601 tctgacgctt gtgccacttc tttactgtga gttacgacaa tcacacattt acctgttttc
     9661 tgggcaagtg atttgagtag ttcgacaata tctccagcag ttttaggatc cagatttcct
     9721 gttggctcat cagctagaat aactggagct tctgagacca aactgcgagc aatggcaaca
     9781 cgttgctgtt gaccacctga taactggaga acattccgct taatctggct ttcatccaaa
     9841 ccaagctcaa gaagtgtatt cttgcttgcc tttttgttga ccaattggat attttccagc
     9901 ggagaaagat aatctatcaa gttataattt tgaaagacca gggaaatatg gtgcatgcga
     9961 tggtaagaat agcccttctt acgaatatcc tctccttgaa aaaggataga accttcaaca
    10021 ggactatcta gaccagcaag tagggacaag agtgtggatt ttcctgctcc tgactcccca
    10081 ataatactgt aaaattttcc gggttcaaaa ttataattga tctgatatag gactgcttca
    10141 gcagtattct tataacggta ggtaacatct tgtaattgta ataaagtcat gatttctcct
    10201 tcttaactaa tagatgataa aatttctttc ggtgattttc taaataagaa taggaaacaa
    10261 agggctacag ataagcaact aagcagaact agaaaaacat aggattctgc aaaagataag
    10321 atgctagttg ataaactgct tgctttggct agtgtatctt gtaagcttac ctgatctcca
    10381 cttgctagta gagtttggag taggtaagtt gtgattccgt ttcctgcaac aaatgctgga
    10441 agcaaagctc caagagatac caaaactacc tctaaacaga attgtaggaa gatcgagctc
    10501 ttgccttttc caagtgcaag taaaatcccc acttcataga cccgttctct caaccagaga
    10561 gacaaaacca gaattaaggc tccagctcct gctatcaaca tcccataaag gaagatggtc
    10621 aggaaggttt ggaaagttgc aactgagtct ttgatttgtt caaaagcctt gttttccttt
    10681 tcgacttggt agccttgatt ttccaaggcc aagttttcta cctgcttcat gagtccgtcc
    10741 atttccttag gattttctac atagaagcgt gctgcactga cttgagcttc actattgccc
    10801 aaaagggttt ggctactttc ataatctgta aagacttgat tttcactgaa gtcagaagac
    10861 aagcctgtga atttctcttg ttttttacca gaaaagatgc cgataatctc aaactctact
    10921 gtttgtcctt ttccagattc agactgacca gcatccaagc caatcttgtc atgaagtgaa
    10981 agaccgttct tcttagccaa ttcttcgtgg ataaggattt tcttggaatc ccctttttga
    11041 aggtgtcgcc cttcttttag attgaaagcc gaactggtaa aggttacatc cttggatgaa
    11101 tcctcaagag ccgttaagct aaccaagtta ttgtctgcag ctgataaatc atcacgctcc
    11161 acgctctgct cgccagtcac tgcttccttg tcttttagtt ttgcgaccgt ctcaagttca
    11221 ggagagacat tttccagccc cttaatcttg cttacagatg ctaggtctga caacttgaat
    11281 gtctgaccat tctctatctt cttaatagaa aaagatgtat tgagtgattt ataaagattg
    11341 ctttctactg ttttgttgga cttcatcaga gtcaaacagg ctgaaattcc ggccaataag
    11401 accaataaaa tcagaaataa aataaaactt ctcagtcgct ttctgctgac ataagcccaa
    11461 gatctttgga ttggattcat ttgtcacctc catatttgta agactattat aaaacccaaa
    11521 tatgaaatgt ttatgaaata cgaaaaaaaa aa
//
LOCUS       DAVKAO010000053        10996 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKAO010000053 DAVKAO010000000
VERSION     DAVKAO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.053"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            11..733
                     /locus_tag="VWX16_001528"
     CDS             11..733
                     /locus_tag="VWX16_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9158856.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VWX16_001529"
     CDS             730..1578
                     /locus_tag="VWX16_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9158857.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VWX16_001530"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VWX16_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9158858.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VWX16_001531"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VWX16_001531"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9158859.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3248..5948)
                     /locus_tag="VWX16_001532"
                     /pseudo
     CDS             complement(3248..5948)
                     /locus_tag="VWX16_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(5950..6651)
                     /locus_tag="VWX16_001533"
     CDS             complement(5950..6651)
                     /locus_tag="VWX16_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158860.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6901..9311)
                     /locus_tag="VWX16_001534"
                     /pseudo
     CDS             complement(6901..9311)
                     /locus_tag="VWX16_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
     gene            complement(9364..10056)
                     /locus_tag="VWX16_001535"
     CDS             complement(9364..10056)
                     /locus_tag="VWX16_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9158861.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10107..10385)
                     /locus_tag="VWX16_001536"
     CDS             complement(10107..10385)
                     /locus_tag="VWX16_001536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158862.1"
                     /translation="MRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKLKEST
                     QKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            complement(10591..10971)
                     /locus_tag="VWX16_001537"
     CDS             complement(10591..10971)
                     /locus_tag="VWX16_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158863.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGSFVLLGTAVNTAATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPYVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
BASE COUNT         3203 a         2523 c         2056 g         3214 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gttgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ccgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat atcctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaatct ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gtatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtc ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcacac atttccggtc aaatcattgg aaaattctga ctgttttaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggta tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaatc ctctgtcaca aagagctccc
     3181 tgctctttgt gacagaggat ttttgaatat tagtaaatag aagttcaatc agctaaaaat
     3241 aactacctta ctctacagat ttcagggctt ctaacatatc aacctttctc agataataat
     3301 tgacgaagaa accaagcaag gtcaaaatga tgcttactgt tgccactggg attacataga
     3361 cttcccagcc tacctgcgga taaaagagaa tagtcgcagg cgaaatcatt tgaatcaaaa
     3421 attggtgtaa atagaaacca gctaccagac caagtacgat tcccacaagg gacagcacaa
     3481 tcgtctcacg gtaaatgtag agggtgactt cattattatg aaaaccgaga accttgatag
     3541 tggagagttc acggattctc tcagctacgt tgatattggt cagattgtaa aggatgacaa
     3601 tagctaatag aaccgatacg atgaccaaga tggtcatggt ctgattgagt gagctagcga
     3661 tagagtcgaa tggctgaagc attttggaca acgctggaca ccgcagattg attcataagc
     3721 aagcccgcct gactttcgat actagttgca ctggtatccc ttaatgagac cagataagtg
     3781 ttggcttggg gtagctgtcc gtaaagttgc tcatagctag cctgactcat ataaataaag
     3841 tgaccaacgt agttctcagt aatagcagcg acctttagtt ccttaccttc aatttctaaa
     3901 gtctgcccaa ccttgacacc tgccagctgg gcgagtttag ctgtaataac gatgccatct
     3961 tttaatgtca gctcctgctg atgatgttga agatggataa agggagtcaa atcttccttc
     4021 tctatcatca taagagtaat gttttgaaga ccagccttgc ctttgaaatc cttgtctagc
     4081 gctttagaat agattttctg gtaggctagt atctcctgcc ctttcaacac ttctgctagc
     4141 tctaccttgt cctgattggt cgcactagga ttttcagaga caagcatctg atactgttgg
     4201 atttgttgaa actgtttaga cggaactcct gctacagaag attggattcc caaacctgca
     4261 aagagcagag ctacagaacc tgccacacca aagattgtca tcaacattct ctgcttataa
     4321 cgaaagatgt tgcgggctgt taccttatga gtaaaactga gacgacgcca gataaaaccg
     4381 atacgctcca ataagatttt agctcctttg acaggaggtt taggaagtag aagctgggct
     4441 gcttcgtcat gaagttccct ccaagccacc agataggctg gtaacacact cgccaaccag
     4501 ctcaagacaa gagctagtaa gctataggtc caatagaact gaatctgagt ttctcccacc
     4561 accatgcctt ttgtaatgac acttgaaatt acactggcta gcaaataatg accaagtata
     4621 ctacctagag ccgttccgac agtcccagct actagtccat aaaggagaaa cttggcgata
     4681 atatccttac tacgataacc caaggcctta aaaattcctg catgagttcg ctcttcgtct
     4741 acaaagcgag tcatggtcgt aaaggtcacc atggctgcta cggcataaag taccacagga
     4801 aagatattgc ccactgctcg aatactggat gaagcattgc tatacataag atagccctga
     4861 ccacctggca tggtctgacg attatagacc tgataccttg gctccgccaa atcgtccaag
     4921 acttgctgat gtttttctaa tttttccttt tcttgggcta gattttgttc agcttgcttt
     4981 agtttggcct cttccttgcc caattcctgc ttggcttggg taagttgagc attagcctgc
     5041 tctctctgaa cttgaggaaa caaggctagt tgactttctt gagcctgtat acgagcttga
     5101 gcagctgcta aacgacgctt gccttcctgc aaattagtct gagccttgtc aagggtctct
     5161 tgccccttgt ctagagactc ttgtccttct tttttcaaaa gttgcagacg taccttgcca
     5221 ttatctgata aaatttgttc aagctcttcc tgatgttgct tggatttttc ttcataagct
     5281 gatgaaaagg catttaaacc tgctaaatct tgatatttca aacgagctat attgtagact
     5341 ttctgatcaa actgactagg taaaatcacc ccataggcta tcagagtccc acttccactt
     5401 cctgcgtagc ccatatctcg ctgggagagg atttcagccg aatccacaaa accagtaatg
     5461 gtataagtat ggtcttttaa agaggaatga ccctcttctt tttctttaaa actaatctcc
     5521 tgtcccacgc tgtattggcc ttgcaaatga gtggccaaag cgatttccct gtctgactga
     5581 ggaagtcgtc cctttcttag ctgaaaggtt gaaattcgct ctggtttgga gtacagccga
     5641 atggcatcct gcccattatc catagtcaca tctgtcaaat agccaaactc gacctctgcg
     5701 ccctccgtct gttttagttc ttcttggtct gcttgatcca agccatagtt agacatgact
     5761 gccaaatcca aggtttgagc agttgttaaa taagcattag ctgtcgtctc catgttggga
     5821 ctggttactt tgaggcctac taaggctaga gatcccaaca tcatcaggat caagatggat
     5881 aaaaaacgcc ccttggagcc tgtgaaggac tgaattaagt ccttccaata agtttttcgc
     5941 ttgatcatgc tagtactcca aactgtcaat atcctgagga tgctggttga gcaccacatc
     6001 cttgacactg gcatcgtgca tatgaatcac gcgatcagca atgggcgcca aagctccatt
     6061 atgagtcacg atgatcaccg tcgctccctt ttgacgagac atgtcttgga gaattttcaa
     6121 aacctgcttg cccgtctgat aatccaaggc tccagtcggt tcatcacaaa ggagaatttt
     6181 aggatttttg gctaccgcgc gtgcaatgga gactcgctgt tgctcccctc cagaaagctg
     6241 ggctggaaag ttattgagac gatgagccag acctacatct gtcaagacct gatcaggatt
     6301 caaggcatct gtcacaattt cagaagccag ttccacattt tccttagctg tcagattaga
     6361 aactagatta taaaactgaa aaacaaaccc cacatcattt ctacggtaat tggtgcgctg
     6421 gtgggaacta taatccgcaa tattaacacc atcaatccag atttcccctt catcattggt
     6481 atccattccc ccaagaaggt taagaactgt tgacttgcct gcacctgaag caccaaggat
     6541 aataaccagc tccccctttt caatctcaaa attcacatca cgattggcca caatctccgt
     6601 gtccccaacc tgataacgct tgtaacagtg tttcatctca atataagcca tcagacccac
     6661 cctctatcaa acaaattata ctcctcgaaa atctctcttc aaaccacgtc agcttccatc
     6721 tacaacctca aaacactgtt ttgagcaacc tgcggctagc ttcctagttt gtgctctttg
     6781 attttcattg agtattactt cctattatca ttataacgct ttttcaacta gttggaaact
     6841 gcctatattc ttaaatcctt attaaaagga agtttcaaat tactgcctta taacatcttc
     6901 ttaaaaataa aattgaacaa gatcaccttc tgctgttgcc accgaaatac aatctttccc
     6961 catgcatttc gcttttattt tttcttgatt cattttaatc acactcttct caatatctgg
     7021 ataagaaact cgcaaatctc ctccactcct tgataaaatg gtcaactgta agagtttttt
     7081 atcttcccag ctcatgctca cttcaaaatg tccacgtgcc attaagcctg aaacagaacc
     7141 tgttgaccaa gcatcaggta gggcagctag aggtaccaga taagctgcat gagactggag
     7201 taacatttct gccatgccac tagtagcacc aaaattacca tctatctgaa aaggaggatg
     7261 gctacaccaa agatttggca aggtggatgt ttttaactgc tctgccaata atttatgggc
     7321 tcgattgcca tctcccaaac gcgcccagag attgatctta ttagccttgg accagcctgt
     7381 gccgccatct ccacgatcat tgaggctagc acgtgccgct tcaatatact cttgtccctt
     7441 gtagctaaag agattgccag gatagagttc cactagatgg gaagcgtgcc gatgctgggc
     7501 ctccactttc tcattttgaa aatactgctc ttcctcctca taccactccc tgattcgacc
     7561 agattgagtg atttgcaaag gatttagtaa atcagacttc tccttaacct cagtcaacaa
     7621 gtcctcatcc agtcccaatt cctgagcagc ctgaataaaa tcatgaaata actgccaaat
     7681 tagagattgg tcataagtat tgccaatcga aatcggccca tgttctgggg aataagacgg
     7741 agaagacacc caacgctgcg cctgctgatc cttatgtaaa aaggcattcc aaaaacgaac
     7801 cgtttccctc aacatgggat aaattttctc tctgagatag tcttggtccc tataaaatga
     7861 ataggcttca taaacggttt gcatcatcca cgcattggca gctggtgacc aaccccaata
     7921 gtaatcccaa ccaggtgccg tccaaccaaa gggagtcgct tgagtatgaa ccaaccaacc
     7981 attttcctca cctttttgag agacgattcc tgcatacttt acagccgcta gacgaccata
     8041 gacacgcaaa tcatctacat agttgatgac cggaaagacc gcctctagga gattggtaac
     8101 ataggctggc caataattca tctgcagatt gacatttaag tgatagtccg aattccaagg
     8161 aggattgtcg accgcattcc agactccctg taggttagct ggtagagcat ctgggcagtc
     8221 tctggacgaa ctaatcaata aataccgtcc atactggaag aacagctcct ccaaagcctg
     8281 cccttcttgt ggcttataat tttttaacaa atcatctgta gtggatgcgt caacatcagc
     8341 ttccaaatcc aattgaacac gctggaataa ggcttggtag tcctcgatat gccttgattt
     8401 caattgggta tagccctttt ctttagctgt gtccaccaag tctatcacct gttgctctaa
     8461 atctagtttc ttgcgataat tgctagcagg attttgggca aaatccgtct tagcggccaa
     8521 gaagagattg gcataactgg ctcctgatat ctgaacccta tctgaccaaa ctctaatatc
     8581 tccatccgtt tcccaagcta gataactagc aaaccgcaga tcattatcct taactcttcc
     8641 cttcatcaag atatgagaat cagtaatatc caacttacac tccttgtaat cagatttttc
     8701 ctgttcatac tttccattag aagccaaatc acaggtcaag gatagttcta tagtaaaatc
     8761 tagagtttcc aacccttcct tagtaaaaca ttgaaccaag agatcatctg gaaaactcgc
     8821 aaaagcttca cgttcaaatc gcgttccctt acagacataa gaagtcgtcg caagtgcctt
     8881 actaatattc agctgtctct gatagtccgt cacctgagac aaagtcgtac cttgctggct
     8941 gaactcaatg tgaatatccc caaaagacag ataggtccca tattgactcg tttttggccc
     9001 aattaggtgc tgctcagcca gttccttagc cagattgtaa tctctcttct ccaaagcctg
     9061 ccgaatctca gctaaaaaac atactgatcc tgaagatttc caccctgata atctgaacta
     9121 tcaggaagtg gacctccaga ccagagactt ttttcattaa attgaatccg ttcagcccct
     9181 ataaggccaa atacttttgc tcctaaagaa ccattgccta tcggtaaagc ttcttcttcc
     9241 caacccatgt aagtggttga agctggttgc ttgtaggcca gtacataatc ttgtttttta
     9301 ttccttatca tacttccata ctaacataaa aagcctagaa atcaattcta gacttacaat
     9361 tttttatttt ccaaggtagt attcagaaac gacgttcaat ttttcgtcga attcgaatac
     9421 caatggtggg aagttaggga tttccacgtc catgatctcg tcatctgaca aacctttgat
     9481 gtgttttaca agggcacgga ttgagttacc gtgagctcct acgaatacgt ttttaccatc
     9541 tttaagagct ggagcgattt tatcttccca gaatggaaga gcacgttcca aagtcacttt
     9601 caagttttca gcatctggga taactgagtc gtcaagtgaa gcgtaacgac ggtctgtgtg
     9661 agctgagtgc tcatcatcac ggtccatgtt tggaggcaat acatcgtatg aacgacgcca
     9721 gatgtgaact tgctcatcac caaattgttc agcagcttca gctttgtttt taccagtcaa
     9781 accaccgtag tgacgttcgt tcaagcgcca tgatttttca actggaaccc acaattggtc
     9841 agaagcttca agagccaagt tagttgtttt gatagcacgt ttcaatactg aagtgtaagc
     9901 ttggtcaaat tcgataccag cttctttgat caatttacca gcgtcaatcg cttgttgtgt
     9961 acctttttca gacaaatcaa catcagccca accagtgaaa aggttagctt tgttccattc
    10021 agactcaccg tggcgagcaa aaaccaattt taccattaga tggattctcc tttaaatttt
    10081 ccgaggtttc ccctcgttta tctattctat tttacacaat ttttcacaaa aaagctagtc
    10141 aaaaccaaaa aggaaaggac tgtttgacaa tgtttggcaa tcacattgtt tttggttgtt
    10201 tctattctat cttcattatt taaccttttt ttctgcgcat cgaataattt ttgagttgac
    10261 tcttttagtt tagtaaacga gtcattattt tccatagctg tgcctttgaa accttcctca
    10321 gctttagtaa aataatcatc aaaaatattt ttataaagat ctatataatc tgcataagtg
    10381 cgcattgatt caatcgcatc gtctgttaca accggtgtat cgaaagtgat tgtttctttt
    10441 gaactattgg atgttgctac tgtcttctca tcattacttg ttgaagtatt ctttgaactt
    10501 tatgttccac atgcaactag aagagaaaga gataatagag ctactcctat accgataaac
    10561 ttttttgttc ttttcatgct aaaatacctt ttattcttct gatttgaatt tcttcaatgt
    10621 tgcaaggaac agagatcctc cgataatagc aagaatcccc ccaacccatc ctaagaatgg
    10681 aatgagacta actccaccag aaacaatcat tagtacatac ggagctgcac ctacacgctt
    10741 atctccttta tagtaaacaa tagcaatgat accaagaaca agatttgcaa tcttcaaggc
    10801 attcaagagt aaggcaacac ctgaaaaagc ccctgcagta gctcctccat taagagttgt
    10861 agcagctgta tttaccgctg ttcccaacaa gacaaaagaa ccaataagaa gtaaaattcc
    10921 tcccacaaga ccgacgatac cactaattaa agcaagtgtt tttaatttca taataaaatc
    10981 tcctttttta tttttt
//
LOCUS       DAVKAO010000054        10800 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKAO010000054 DAVKAO010000000
VERSION     DAVKAO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.054"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            87..251
                     /locus_tag="VWX16_001538"
     CDS             87..251
                     /locus_tag="VWX16_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9158864.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            335..901
                     /gene="amaP"
                     /locus_tag="VWX16_001539"
     CDS             335..901
                     /gene="amaP"
                     /locus_tag="VWX16_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9158865.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            913..1083
                     /locus_tag="VWX16_001540"
     CDS             913..1083
                     /locus_tag="VWX16_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9158866.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1122..1730
                     /locus_tag="VWX16_001541"
     CDS             1122..1730
                     /locus_tag="VWX16_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9158867.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1761..1964
                     /locus_tag="VWX16_001542"
     CDS             1761..1964
                     /locus_tag="VWX16_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9158868.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2074..2346)
                     /locus_tag="VWX16_001543"
                     /pseudo
     CDS             complement(2074..2346)
                     /locus_tag="VWX16_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2550..2837
                     /locus_tag="VWX16_001544"
     CDS             2550..2837
                     /locus_tag="VWX16_001544"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9158869.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3115..3885)
                     /locus_tag="VWX16_001545"
     CDS             complement(3115..3885)
                     /locus_tag="VWX16_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9158870.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3900..4748)
                     /locus_tag="VWX16_001546"
     CDS             complement(3900..4748)
                     /locus_tag="VWX16_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9158871.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4745..6220)
                     /locus_tag="VWX16_001547"
     CDS             complement(4745..6220)
                     /locus_tag="VWX16_001547"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9158872.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKTYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6543..7898)
                     /locus_tag="VWX16_001548"
     CDS             complement(6543..7898)
                     /locus_tag="VWX16_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9158873.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7908..8225)
                     /locus_tag="VWX16_001549"
     CDS             complement(7908..8225)
                     /locus_tag="VWX16_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9158874.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8242..8559)
                     /locus_tag="VWX16_001550"
     CDS             complement(8242..8559)
                     /locus_tag="VWX16_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9158875.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8574..9470)
                     /locus_tag="VWX16_001551"
     CDS             complement(8574..9470)
                     /locus_tag="VWX16_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9158876.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9473..10735)
                     /locus_tag="VWX16_001552"
     CDS             complement(9473..10735)
                     /locus_tag="VWX16_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9158877.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         3714 a         2184 c         1668 g         3234 t
ORIGIN      
        1 aaaggagggt aggcagtgtt gtgatcaatt attgctggag gtcttattgg tctcttggca
       61 ggtaaaatca ctaaaaaagt agttctatgg gaatcatcgc aaatgtattc gctggtttag
      121 tcggggcata tgcaggacaa tctcttttag gtagttgggg tccatcaatc gctggaatgg
      181 ctttgctccc atctattgta ggtgcagcga ttgtgattac tgtagtgtca ttctttacag
      241 gtagaaagta aacttttcgc cagtaaagtt agcaaactat ttttaaatca atgacgggaa
      301 aaatagttta aatgttaaat cgaaaggatt gtatatgtca aaagcaaaga aaatatgttt
      361 cattattttc tgtattttaa tcttgacaat tttccttcct gttttgatag attatcatca
      421 agttagtgat ctaggtattc atctacttag ctggagacag aactccgtag ttgaattcta
      481 tcttgctaga tatgtctttt gggggacagt ggttctatca actttagttt tattatccat
      541 tttagttgtg atgttttatc ctaaacgtta cttggaaatc caacttgaaa ctaaaaacga
      601 tacattaaaa ttaaagaatt cggcaatcga aggttttgtt agaagtttgg tgagtgatca
      661 tagattgatc aagaacccaa ctgttcatgt aaatttacga aaaaataaat gtttcgttca
      721 tgtagaaggt aaaattcttc cttcagacaa catcgctgac agatgccaaa taattcaaaa
      781 tgaaataact aatggattga agcagttttt tggtattgag cgtcaagtaa aacttgaagt
      841 tgcagtaaaa aattaccaac caaaacctca aaacaaaaag actgttagtc gtgtgaagta
      901 aggaagtaaa aaatggaatg gcttaaacaa tatcgatatc caattatcgc tggtctcata
      961 ggcgtatttc tggcttgttt gattgtctcc tttggcttct tcaaaacaat atttgtattg
     1021 attttaggag cactgggagt tgcagctgga ttatatatcg aaaaaaacta tatagataaa
     1081 taaaaaaata aaaattacta atttaattaa aggagtttca tatgtcaaac gaaaaaaaca
     1141 caaacactaa cgtagaaaag aaagatgcta ctgttgtagc tcacgaaatc aaaggggagc
     1201 ttacttacga agataaagtt atccaaaaaa tcattggtct ttcactagaa aacgtttcag
     1261 gtcttttggg aatcgatggt ggtttcttct caaatcttaa agaaaaaatc gttaacagcg
     1321 atgacgtaac aagtggtgtt aacgtagaag ttggtaaaac acaagttgca gttgacttaa
     1381 acgttattgt tgagtaccaa aaaaatgttc cagctttata ttcagaaatc agagaaatcg
     1441 tatcttcaga agttgctaaa atgactgact tggaaattgt tgaaatcaac gtaaacgttg
     1501 tcgacatcaa aactaaagaa cagcatgaag cagactcagt aagccttcaa gatcgcgtat
     1561 ctgacgttgc tgaatcaaca ggagaattca cttcagaaca attcgaaaaa gctaaatctg
     1621 gtcttggatc tggtttctca actgttcaag aaaaagttag cgaaggtgta gaagctgtta
     1681 aaggtgcagc aaatggtgta gtatctcacg aaaacactcg tgtaaactaa gataaaataa
     1741 atataacagg agaaattatc atgtcagtag aagaaaaatt aaatcaagct aaaggttcta
     1801 ttaaagaagg tgttgggaaa gccatcggtg atgaaaaaat ggaaaaagaa ggagcagctg
     1861 aaaaagttgt ttctaaagta aaagaagttg ccgaagacgc taaagacgct gtagaaggtg
     1921 ctgtagaagg tgttaaaaac atgttgagtg gcgacgataa ataaggttaa aagttacttt
     1981 atctttttgg taacattagt caaaagagtc tgagtcaaga tgattctcag aaaacaaaaa
     2041 gctagagatt cccaattgcg gaactctagc tttttaattt tgcctctttc tcttattata
     2101 tttcagcagg ttgttggcca tgagtatgaa tcccatgtca attctcactt gacgcttacc
     2161 tctcagatga catctcttat aacccaaaca aacctttatc tgcccaaaga cagatttcat
     2221 atcaatctta cgtttagcga aaatttgtct acccttggaa gataaaagtg cctgatattc
     2281 tttagttttt aaacactggt aacgttcatt catatacagt ctcttttgag gggctgattc
     2341 aggttcataa tcgcagtcaa cattgatttc aaggctgttt gctttctatc tccccttttt
     2401 catgatatat tttcgtacag gtgcttcaat agtttctata gtaaaatgaa ccaaaaatag
     2461 tacacgttgc ggtataatgt tcttatggca tattcattag attttcgtaa aaagttctct
     2521 cctatttgtg agcgaacagg tatagtcaat tgattgtcag tttgaagacc aagagtcgta
     2581 aggcaaagga tatggcagaa tccatccaag ggtggctggc acaatttcta gtgaacctct
     2641 ttaaatctat tacgtttgac tgtggaaaag agttttcaaa atggaaagat atttccaatc
     2701 atcacgatag tgagagcttt tttgcgaatc taggatgtcc aagacagcgg tgtctcaacg
     2761 aacattcaaa tagactttta aggtgtcatg acttgccaaa gcagacagat ttcaacgaag
     2821 tcagtcaaga attttaggct gggtaaaaac tcaatttcag gagaaaatga aataaatatt
     2881 cccacaataa aatgcatagt atcaaagttt ttccaacacc tgatattatg cattttttaa
     2941 ttttaaagac tttttcccag cctcaagaaa tcacatccca cctaaatcct ttggatacca
     3001 aatacctcat caggtatttt ctgagttggt aagactgtct agcttaattt gacaaatgga
     3061 gggcgttaaa tccagttttt acaggaagtc tatttaaata ttttcacact gaccttacaa
     3121 aatatgaaat aaattatata aagtcattcc catgacaaag aataacatcc ctttaaaaca
     3181 gtatcttacc caattttcag gtaagacttt tgatacaacc gtccccaata cccctccgca
     3241 tatagaagca agaaaaataa ctggaacagg tgctaaatga tactgattta aacctgtaac
     3301 cacaacaatt gtagccaaat gagacaactg ggaaaagaaa ataattgcaa gagaatacat
     3361 cgttgcttct tttatcgaaa tagaaaaaaa caccatcaat aaagatacat tgagcggacc
     3421 accaccaatc cctaaaaaac tagaaatcaa gcccaataga agaccacaaa tcatatagaa
     3481 tcctatctta tcaaattgat aagttttttt aaaatctgtt aaaacaaatg atacaagtag
     3541 catcacaaat agtaaaatca tttgaacaat ggatactagc cgtactgata attgagataa
     3601 caaaacttga aaaataagat gacctagaaa ccctcctaaa acagaagaaa aggataccgt
     3661 aaaaatcaat ctccatttta tctctttact ttgtgaaaat cttttggttg ttgaaataat
     3721 cgccattata aaaacagaga aactagaata aaaagcaata tcactgaccg aatggtatcc
     3781 aaaactatcc ataagtggct taattataat tccaccaccc atgcctgata tagaacccaa
     3841 tccactagct attataatga ttagaaaata aataatctca accacttttt aagtccatcc
     3901 tattctttca aacatctaaa acctagatgg ctactagtgg aagtagaaat acatccattt
     3961 ctagcagcta ctcggtatcg attacaataa gaacaatgac ataaaaagga acctcctctg
     4021 atagcatact cctcatcttg tatcccatat tttggtaact catagtcatc ttcattaaaa
     4081 ctagctaata aggtatatct cggattgcga caccattccc aaacattccc aatcatttga
     4141 tatagaccaa aatcattagg taaaaattca tagacaggag ccgttcctat aaatccatct
     4201 aaggcagtat tttcataagg aaatcttcct tgccaagtat tggcatgata atatccacct
     4261 tctaaaagag tatctcccca aggatattca gattgtcttc ctcctctagc agcatattcc
     4321 cactgtgctt cagtcggtaa agacattccg ctccaattac agaaagccaa tgcatcttct
     4381 aaagcaacat gaactactgg atggtcttct aaacccacta gattactgtt ctcaccgtag
     4441 ggatgtttcc agcaagcgtt cgaaacttgt aaccaccacg gagccccagc aggatgagga
     4501 tatccctccc tttctgcctc cggaacgaac aatataaaaa caaaagacca ctcttgtcgc
     4561 tcagctagtg ttttatagcc tgtttctttc acaaattgag caaattcttg attcgtaact
     4621 ggagtatccg caatggaaaa atcctttaca ctaacagcga cttgaggacc ttcaaaatca
     4681 tctaaaaagc cctcttcaga atttgttccc atcataaatg tcccaccctt gatttttttc
     4741 attttcatga ttgactatcc cccgttttcg ttagagttgg cgttatttta gaaagttcaa
     4801 caggaactaa ctcttcatcc acaacaaaac cttcttctct atatctaaga aagtctacta
     4861 attttttctt catctgacga acaataggtt gatatttttc acttggatac aaatcattca
     4921 tttcatgcgg atcttttttc atatcaaata actgataatg atttaaaaca ggaaaccaga
     4981 taaatttcca ctggtctgtc aaaatatatt gactagaatc ttttcctaaa gcatgttcac
     5041 catgaaattc tgttcgccac ccctcgtact gtccaaataa taaattctta acactccgtc
     5101 catctagttc atctgtagta gttcctcctg ccaaatccac gagtgaagga aaaatatctt
     5161 gaattttcac taattgcttg attgttcccc tattaccagc tatcaatcca gccggatcgt
     5221 aaataaacga aggaatgtgg atacttccct gataaggata tccctttcta aacagataat
     5281 gttcaccaag ctgatctcca tgatccgaaa caaaccagat aatcgtatct ttatcatgac
     5341 gaaattcttt tagggcagtc aaaaagcgac taatttgatg gtctatatga gtaatcaatc
     5401 caaaataagc agcaaccatt cttctttgat catcttcctt taatttccct cttaaagcat
     5461 aaatagacgg aatttgcttt tctaatactt cccaattccc gatatgcaaa tcgagaaatt
     5521 gaggcaagcg ttccatatac atatcaaaat agtattttgg gggatttaat ggagcatgtg
     5581 gtttttcaaa agacattttg agaaaaaaag gaaccgttgg atctcttctt tgtaaaaatg
     5641 aaatggactc actcacaacc cagtttgttg gatgtaactt ctcatcttta tcccaaggtc
     5701 tagcttccca ggaattacaa tccattccgt catcaatcaa atcaacatca taaccaactt
     5761 taccctttaa aaaggctaga tagtcactag cataatcaaa ttgacttcca taagtcttat
     5821 catactttct atcaacatgt agatacccat cgtgaagtaa tacatgatca aacccaagtc
     5881 tctgtctgga aggaaaaaca tgcatcttac caatacactc tgtttgataa cccatatctt
     5941 taaagacttt tggaagagta ttagtaaaat tccaaggcac ctcatcttga taaccaacac
     6001 gtccactttt atcttggtct aagccagtca ataaagctgc cctagcagga acgcaagaag
     6061 gaactggaga ataggcattt tcaaaattat aacctacact agccatcata tctaatgttg
     6121 gagtgctaac tagcttatcc ttactattca aggataaggc gtctgctctc atttgatcta
     6181 caacaatcaa aataatattt ggttgttttg tctgaaccat aaaatctcct ttctaatatg
     6241 gcaaaagagg cacaagaaga tatctacctt tactgcaccc ctttctatat caatctctct
     6301 atataaagca ataacattct tgttatgttt tatagaacaa tggactaaaa tatgactaaa
     6361 tcgattagga aattcaaatc attttctagt actgttttag aagttacagt gtactattcc
     6421 aacttcaata aattataaac ctttgtctaa taacaatttt agtggagata agaaatccta
     6481 cacctaactc atcttacacg taatctattt ctattttatc acaaaaaacg caagtaagac
     6541 cattaactca attcagtttt atctgccatt ttcacaaatg ggaaataagt caagacacta
     6601 ataatcaaac aaacaactga taagatgatg gcacgccaat caaatgctgt agagaagaaa
     6661 ccatataaaa ttggaggcat tacccaagta acattttgtg taacaggtga aacaagaccc
     6721 cagcttgttg cccagtaagc taccgttgcc atgaaaaccg ggctaagtac aaatggtata
     6781 aatagcaaag gattcaagac aactggtaaa ccataattta ccggctcacc aatattaaac
     6841 agaactggtg ctagaccaag tttagcaact tttcgataat gactgtttct tgaaaaaatt
     6901 aaaatagcaa gtactaatcc taatcctcca aaccagacaa acgccccaaa agacccactt
     6961 gtccatatat aaggaatcgg ttcacctttt tggaaagcat ccagattcgc taacatagca
     7021 actccaaata gcccttccat gatgggagcc aatacatttc ctccatggag accaaaaaac
     7081 cagaataact tattcaaaaa gatcatcaga ataactgcaa agaaactttg agacaaacct
     7141 agtaatggcg tttgtaacac cttgtaaacc caatcaatca ataagtcatt gctaagtaaa
     7201 tggaaaacat aagtcaagat ggctactata tacatcgcca taaatcctgg aatgatagaa
     7261 gtgaacggct tagcaatcgc agggggaact gaatctggta acttgattac ccagttcttt
     7321 ttcattactt tacagaaaat aatagaggct aaaaatccaa tcatcatggc tgtaaagtag
     7381 cctctggcat taatatggtt tcctggaatc acattcccaa tagttaccat cagattttta
     7441 ccatcaaatg ctagattatc aattccttgt aagatttgat ctaatttcac atctcctaca
     7501 tttgccaaag ggaaactctt tgtaactgta cttccaatcg aaatgacaaa cgaagcaagt
     7561 gataccaaac cagcagaaac tgtatcaacc ttgtaaatct tagcgatatt cactcccaag
     7621 caatagatga acaacaagga aacaattggt atacttccct tgaataccaa attattgatg
     7681 tcaacaagcc actgaaaggt tttcgtaata ctttctaggt gaaattgttg tggtaaatcc
     7741 actagaaaag catttaataa caaagcaatg gaacctgtca taataacagg catagtcccc
     7801 acaaatgaat cacgtaaagc aactaaaaac ttctgattcc caactttcat tgcaattgga
     7861 gaaagttttt cttgcatttt atccaacatt gtattcatac taaactccta atttttttca
     7921 aatcgttcgt atacttctac cacttctttc gccaagtcaa caaaagtcag actcgtcatt
     7981 aaatgatctt gcgcgtgtac catcaagagg ctaattgatg tttcttcccc ctgtgcctct
     8041 cttgtcaaca tttctgtttg agccttatgc gcctgtaata aggcagcatt cgcatcagct
     8101 aatcttcgat tggctttaga gaaatcccct ttttttgctg cctgaatagc ctccatagca
     8161 ttacttttgg cttccccacc atacataatt agccccatta cagattctaa attactttca
     8221 tccataacat tctcctcaat cttagtctaa taaactttca gctaaatcta agactttaga
     8281 accattcatc attccataat ctgtcatcgg aataaccgcc acaggaatct gtctatcttt
     8341 tagtttttct tgaaagtccc ctagtaaata gcgaacttga ggacctaaaa gcaacacatt
     8401 tacttctttt gttgctacaa tttcttctgc ttcaggagct ggaactgcaa aaatttctgc
     8461 atccaacccc ttatcttctg ctgccttttg catctttgtc actagcagac ttgtactcat
     8521 acctgctgca catgctaaca taattgttac tttagccata gcttactcct ttatcaccta
     8581 tttactgacc tagccaagcg agatactgta tgtctcactc ctctaattgc cttgctcaat
     8641 gcataaatat tatcgactgt tgccagtccc ccatatcctg cgtcacctat atgttgaata
     8701 tcaactccac aaattttatt tctaagtgca atttccttaa tagtatctgt atcagatgtt
     8761 tcttggctag taccaatagc actcaataca agaccaccct tactatgaac gagatcaacg
     8821 acttcacgca actcttggtc atgaaaggct ggaacggttc caactgctgg aacaagtatc
     8881 acatccgcac ctgcttccaa caattgctct gcgacagaaa gctctgccac aggctcattc
     8941 actcctgccc cgtgcatctt acctgcaata atcaaaccag aaaagttttc cttagcagtt
     9001 tgaacagcct taatgatttc tcgattgcta actcctgttc caggatttcc agtcaaacag
     9061 acaaagtcaa aacctaactc ctctattcgc ttcaatgttt caacactagc aacacgacct
     9121 gcaacaattt cctgtgtttc ctctagcatc tttgcagatg ggtcaatcgg ttccaaattt
     9181 acaccaattg gacaagcaac aagctggtgt aagcgatgaa taacatcatc acctgaacta
     9241 tccaaagcat aaattttagg ctcaaaaaca tcaacacaat tcaataaaat catatcagct
     9301 ccaaaagctc tagcaatttc agaattagtt atatccccaa caaatgtctc acgagtaact
     9361 acattttcag atagaacaac tctaccttca cttgctaaaa tactttgttt taattcttca
     9421 gcattcatct gtaatatctc agatggattt gcactaatta aacgtttcat tctcaatcct
     9481 ccaataactt aaaccgtttt tcaatagcta ttacaaatgg taaataaagt aagacatcca
     9541 aaacaatcaa tacaatttgt aataatgaac cagaaatact tcctgtagca agtaaaccac
     9601 ttataacagg tggcatagtc caacctggat ctgtataagt tagcggaacc aaacctgatg
     9661 ccattgctcc ccacgctact agtaagttaa acattggagc taagataaat ggaactagta
     9721 acaaaatgtt caaaacaata ggaacaccaa acattgctgg ttcatttata ttaaataatc
     9781 caggtataac agttataggt gccaaagttt ttagttgttt actagctttc ttcttatgtg
     9841 ctaaatatcc taacgcaaga actaaaccta tggttgcacc accgccacct atgaatacaa
     9901 aattatccat aaatacacta gtaaaaatat ttggaagagt ttctccagtt tgataagcta
     9961 ctttatttgc atctagatta gctaaccata acggtttcat aactgcatta acaacatttc
    10021 ctccgtgtaa tcctacaaac caaaaactac tattaaggaa aattaagata agaagaccaa
    10081 taacattatt tcctaaaagt ccaagtggac ctcccaaagc tacttgagca atttcatgaa
    10141 gattaggtaa acttaattta tctaaagttg cataaacaat caaccaacca acaatagtca
    10201 ctgcaccagg aataatggca ctaaagcttt tagatactgc tggtggaaca ccatccggca
    10261 tttttatctg tatattatga tttataaacc attggtagat atagccatta atcaatccta
    10321 aaatcatagc aacaaataaa ccttttgatg ccatgaaagc ggtaggcatt cctgctccag
    10381 cctctcctgt aatgaaaggc gtaactgtaa taaaagatga taatgacaca ataccagcag
    10441 gaataccatc aactttgtat tgtctagcca taaagtaagc gataccaaaa ctagctacaa
    10501 gaccaagtaa tccaaaacta ctatcaacac ccttccataa atagtcagca acaccaatat
    10561 cacccagata tttttcccat cctggaatag gaaaacttgc aataaccatt agtaatgaac
    10621 ctatcaacaa taaaggtata gttagagtaa taccgtctcg aatagaaacc aacgctttat
    10681 tattacccaa tctagccgct actggaagta acttttcttg taataatttt tccataacct
    10741 tttcctctta aatatatttt attatcaaaa gttatcaatt atctttattt tttgacgtca
//
LOCUS       DAVKAO010000055        10699 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKAO010000055 DAVKAO010000000
VERSION     DAVKAO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.055"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            65..562
                     /locus_tag="VWX16_001553"
     CDS             65..562
                     /locus_tag="VWX16_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9158878.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            562..2217
                     /gene="dnaX"
                     /locus_tag="VWX16_001554"
     CDS             562..2217
                     /gene="dnaX"
                     /locus_tag="VWX16_001554"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9158879.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2245..2439
                     /locus_tag="VWX16_001555"
     CDS             2245..2439
                     /locus_tag="VWX16_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9158880.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2589..3359
                     /gene="sufC"
                     /locus_tag="VWX16_001556"
     CDS             2589..3359
                     /gene="sufC"
                     /locus_tag="VWX16_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9158881.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3387..4649
                     /gene="sufD"
                     /locus_tag="VWX16_001557"
     CDS             3387..4649
                     /gene="sufD"
                     /locus_tag="VWX16_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9158882.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKVTANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4660..5886
                     /locus_tag="VWX16_001558"
     CDS             4660..5886
                     /locus_tag="VWX16_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9158883.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDRVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5873..6313
                     /locus_tag="VWX16_001559"
     CDS             5873..6313
                     /locus_tag="VWX16_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9158884.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQVQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6367..7779
                     /gene="sufB"
                     /locus_tag="VWX16_001560"
     CDS             6367..7779
                     /gene="sufB"
                     /locus_tag="VWX16_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9158885.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8010..9251)
                     /locus_tag="VWX16_001561"
     CDS             complement(8010..9251)
                     /locus_tag="VWX16_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9158886.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIEQI
                     GNQSSQSVQFTPDSKAIPAPLEARTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9354..10463
                     /locus_tag="VWX16_001562"
     CDS             9354..10463
                     /locus_tag="VWX16_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9158887.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3168 a         1955 c         2330 g         3246 t
ORIGIN      
        1 tcctttgttt actaaacaag ggatttttct tttgtctcta ctcctttttt gatataatag
       61 ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg ttaaatgaag aattgtcctt
      121 cctattggaa ggcgaaacca atgttttggc taatctttcc aacgccagtg ctctcataaa
      181 atcacgtttt cctaataccg tatttgcagg cttttatttg ttcgatggaa aggaattggt
      241 tttaggcccc ttccaaggag gtgtttcctg catccgtatt gcactaggca agggtgtttg
      301 tggtgaggca gctcactttc aggaaactgt tattgttgga gatgtgacga cctatctcaa
      361 ctatatttct tgtgatagtc tagctaaaag tgaaattgtg gtgccgatga tgaagaatgg
      421 tcagttactt ggagttctgg atctggattc ttcagagatt gaggattacg atgctatgga
      481 tcgagattat ttggaacaat ttgtcgctat tttgcttgaa aagacagcat gggactttac
      541 gatgtttgag gaaaaatctt aatgtatcaa gcactttatc gaaaatatag aagtcaaaac
      601 ttctcccagt tagttggtca agaagttgtg gctaagactc ttaaacaagc ggtggagcaa
      661 gagaaaataa gtcacgctta tcttttttct ggtcctcgtg gaacgggaaa aaccagtgtt
      721 gctaaaatct ttgccaaggc tatgaactgt cccaatcaag tgggtggcga accttgcaat
      781 aactgctata tttgtcaagc agtgacggac ggtagtttag aagatgtcat tgaaatggat
      841 gcagcttcta ataatggggt agatgaaatt cgcgaaattc gtgataaatc tacctatgcg
      901 cctagccttg ctcgttataa ggtttatatc atagatgagg ttcacatgct gtctacaggg
      961 gcttttaatg ccctcctaaa gacgctggaa gaaccaacac agaatgtagt ctttattttg
     1021 gccactactg aattgcacaa gattcctgct actattctat cccgtgtgca acgttttgag
     1081 tttaaatcaa ttaagacaca ggatattaag gaacatattc actatatctt agaaaaagaa
     1141 aatatcagtt ctgaaccaga ggctgtggaa atcattgcca gacgggcgga aggtggaatg
     1201 cgggacgcct tgtctatttt ggatcaagcc ctgagtttga cacagggaaa tgagctgacg
     1261 actgctatct ctgaagaaat tactggcacc attagcctat cagccttgga tgattatgtg
     1321 gcggccttgt ctcaacagga tgttcccaaa gctttgtctt gcttgaatct tctttttgac
     1381 aatggtaaga gcatgactcg ttttgtgacc gatcttttgc actatttaag agacttgtta
     1441 attgttcaaa cagggggaga aaatactcat catagttcag tctttgtaga aaatttggca
     1501 cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa cagtgaattt agcagatatt
     1561 aagtctagtt tgcagcccaa gatttatgct gaaatgatga ccgtccgttt ggcggaaatc
     1621 aagcccgaac cagctctatc aggagcggtt gaaaatgaaa ttgctacgct gagacaggaa
     1681 gttgcccgtc tcaaacaaga gctttctaat gcaggtgcgg ttcctaaaca agttgcacca
     1741 gctcctagtc gaccagctac gggcaaaaca gtctatcgtg tcgatcgcaa taaagtgcaa
     1801 tctatcttac aagaggccgt cgaaaatcct gatttagcac gtcaaaatct aattcgtttg
     1861 cagaatgcct ggggagaggt aattgaaagt ctaggtgggc cggacaaggc tctgctagtt
     1921 ggttctcaac cggttgctgc caatgaacac catgctattc ttgcttttga gtctaacttc
     1981 aatgctggtc aaactatgaa acgagacaat ctcaatacca tgtttggtaa tatcctcagt
     2041 caggcggcag gtttttcacc tgagatttta gctatttcca tggaggaatg gaaagaagtt
     2101 cgcgcagcct tttcagccaa agccaaatct tctcaaactg aaaaagaagt agaagaaagc
     2161 ctgattccag aaggatttga atttttggct gataaagtga aggtagagga agactaaaga
     2221 aagatttcat gatacaataa gtttatgaat aaacaacaat ttattattat ggcgctattt
     2281 acagctgctg agacctattt tttcaatgaa gcctggatga ctggccgcta tattatggca
     2341 gccttttggg caattttact ctttagaaat ttccgagtca gttatgtgat gggtaaaatt
     2401 gttgatgtca tcgatcagca ttttaatagg aaagactagc cctcagcttc cagacaaaat
     2461 caaagccttt taggcttttt tttgttatac tagaaaagta tatttataga atttttgctc
     2521 tatttctggg gaaatcagac gtttttctag taagtactgt aaaagttttg aaaaagaaag
     2581 gaactatcat gtcagtaata gagatcaaag atcttcacgt tgagattgaa ggaaaagaaa
     2641 ttttaaaagg ggttaacctg accctgaaaa caggagaaat tgccgctatc atgggaccaa
     2701 atggtacagg taaatcgact ctttctgccg ctatcatggg aaatccaaac tatgaagtaa
     2761 ctaaaggtga agttttgttt gatggcgtaa acatccttga gttggaagtg gatgagcgtg
     2821 cgcgtatggg acttttcctt gctatgcaat acccatcaga aatccctgga attaccaatg
     2881 ctgagtttct tcgtgccgct atgaatgcgg gtaaagaaga tgatgagaag atttcagttc
     2941 gtgagtttat tactaagcta gatgaaaaaa tggaattgct caacatgaaa gaagaaatgg
     3001 cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa aaaacgcaat gaaattcttc
     3061 aacttttgat gttggagcca acatttgctc ttttggacga gattgactca ggtcttgata
     3121 ttgacgctct taaagttgtg tctaaaggtg tcaatgccat gcgtggtgaa ggttttggtg
     3181 ctatgatcat cactcactac caacgtcttt tgaactatat cacacctgat gtggtacacg
     3241 tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga attggctgcg cgtttggaac
     3301 gtgaaggata cgcaaaacta gctgaagaac ttggctacga ctacaaggaa gaattgtaat
     3361 tccctcgtat cttttaggag aagtaaatga ctagagaaaa tattaaactt ttttcagaaa
     3421 tgcacgctga accaagctgg ttggctgatc tccgtcaaaa agcttttgac aagattgaga
     3481 ctttggaatt accagttatt gagcgtgtca aattccaccg ttggaatctg ggtgatggaa
     3541 cgattacaga aaatgagcca tcagcaaatg ttccagattt cacagcttta gatcatcact
     3601 tgaagttggt gcaagtagga actcaaactg ttttcgaaca aactccagtt gagttagctg
     3661 aacagggtgt tgtcttcaca gactttcact cagctttaga agaaattcca gagctgatcg
     3721 aagaattctt catgtcatct gttaagtatg atgatgacaa gttggcggct taccacacag
     3781 cttactttaa cagtggtgct gtactctata ttccagataa cgtagaaatc acagagccaa
     3841 ttgaaggaat tttctaccaa gatagcgata gcaatgtgcc gtttaacaag catattatga
     3901 ttatcgctgg taaaaattct aagattagtt atctggagcg tttagagtca cgcggtgaag
     3961 gaagtgacaa agtaactgcc aatatcacag tggaagtgat tgcacgttct ggtgcgcaag
     4021 tcaagtttgc tgctatcgac cgtctaggtg aaaacgtcac tgcctacatt agccgtcgtg
     4081 gtaaattagg caacgatgca agtattgact gggctatcgg tgtcatgaac gaaggaaatg
     4141 tcgttgctga ttttgatagt gacttgattg gtaatggtag ccatgctgac ctcaaggttg
     4201 tagctctttc aagtggtcgt caggtacaag ggattgatac tcgtgtaact aactatggct
     4261 gcaactctat cggaaatatc ctacaacatg gggttatcct tgaaaaagca actttgactt
     4321 tcaatggtat cggccacatc atcaagggtg ctaagggagc agatgcgcaa caagagagcc
     4381 gtgttctcat gctttcagac caagcgcgtt cagatgctaa cccaattctt ttgattgatg
     4441 aaaatgatgt cactgcagga cacgcagcgt ctatcggaca agttgatcca gaagacatgt
     4501 attacctcat gagtcgtgga ttggataagg caactgcaga gcgtttggtt gttcgtggtt
     4561 tccttggatc tgttatcgtg gagattccag tcaaggaagt tcgtgatgaa atgattgcaa
     4621 ctatcgaaga gaaattgtca aaacgctaag gggaagccta tgttagatgt agaagcgatt
     4681 cgcaaggatt ttccaatttt agatcagatt gtcaatgatg aacctctggt ctatctggac
     4741 aatgctgcga cgacacaaaa accactagta gttctgaaag ctattaacag ttactatgag
     4801 caggacaatg ccaatgttca ccgtggtgtc cataccttag cggaacgagc gacagcttct
     4861 tatgaagctg ctcgtgaaac cattcgtaag tttattaatg caggctctac aaaggaagtt
     4921 ctctttacca gaggaacgac aaccagcctt aactgggtgg cacgctttgc tgaggaaatt
     4981 ctcgctgagg gagaccgggt cttgatttca gtaatggaac accattctaa tatcattcca
     5041 tggcaggaag cttgtcgaaa gactggagca gagcttgtct atgtctatct taaagacggt
     5101 gccttggata tggaggattt gcgagctaaa ttgactgata aggttaaatt tgtttcccta
     5161 gctcatgcct ccaatgttct tggtgtggtc aatccgatca aggaaatcac tcaattagcc
     5221 caccaagttg gggcaattat ggtagtggat ggtgctcaat ctacacctca tatgaagatt
     5281 gatgtccagg acttggatct ggactttttc gccttttcgg gtcacaagat ggctggtccg
     5341 actggtatcg gtgtccttta cggcaaagaa aagtatcttg agcaaatgtc tccagtagaa
     5401 tttggcggcg agatgattga ttttgtctac gagcaatttg ctagttggaa ggaattgcct
     5461 tggaaatttg aggctggaac gccaaatatg gcaggagcta ttggacttgc gactgcagtt
     5521 gattatctgg aaaagattgg tatggatgcc gttgaagctc atgaacagga attgattgcg
     5581 tacgtctatc caaaactgca ggcaattgag ggattgacca tttacggttc tcaggatttg
     5641 gctcaacgtt cgggtgttat tgcctttaac ctaggtgatc tccatcctca cgatcttgcg
     5701 acggctctgg attatgaagg agtggctgtt cgtgctggtc accattgtgc gcaacccttg
     5761 cttcagtatt tggaagtccc agcaacagct cgtgcaagtt tttatatcta caataccaag
     5821 gcagattgcg acaaactagt cgatgcccta caaaagacaa aggagttttt caatggcact
     5881 ttctaaacta gatagccttt atatggcagt ggtagcagac cattcgaaaa atccacatca
     5941 ccaagggaag ttagaagatg ctgagcaaac cagtctcaac aatccgactt gtggggatgt
     6001 catcaacctc tctgtcaagt ttgatgcaga ggaccgtttg gaagatattg cttttctaaa
     6061 ttcaggatgc acgatttcaa ctgcttctgc tagtatgatg acagatgccg ttttaggaaa
     6121 aaccaaacaa gaaattttag aactggcgac tattttttct gaaatggttc aagggcaaaa
     6181 agatgagcgt caagtccaac ttggagacgc ggcattcttg tcaggtgttg ccaaattccc
     6241 tcaaagaatc aagtgtgcaa ccctagcttg gaatgccctt aagaaaacaa ttgaaaatca
     6301 agaaaaacag taagacaagt ttcttttgtc ttatgaatta ttagaaatga agaaagaaag
     6361 gatactatgg ctgaagaaag agtagaacca aaaccaattg accttggtga atataaattt
     6421 ggtttccatg acgatgtaga gcctgtctta tcgacaggaa aaggactcaa cgaaggtgtt
     6481 attcgtgaat tatctgctgc taagggtgag cctgagtgga tgttggagtt ccgtttgaag
     6541 tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg gagcagactt gtcagagatt
     6601 gactttgatg acttaatcta ctaccaaaaa ccatctgaca aaccagcccg ttcttgggat
     6661 gatgtacctg aaaagattaa agaaaccttt gaacgtatcg ggattccaga agctgaacgt
     6721 gcttatttag caggggcttc tgcccagtac gagtcagaag tggtttacca caacatgaag
     6781 gaagagttcc aaaaattagg tattatcttt acagatacag attccgcact caaggaatac
     6841 ccagacttat ttaaacaata ctttgcgaag ttggtaccgc cgacagataa caagttggca
     6901 gccctcaact cagcagtatg gtcgggtgga acttttatct acgtgccaaa aggtgtcaag
     6961 gtagatattc cacttcaaac ttatttccgt atcaataacg aaaatatagg tcagttcgaa
     7021 cgtaccttga ttatcgttga tgagggagca agcgtccact acgtagaagg atgtacagca
     7081 ccaacatatt caagcaatag cttacacgct gccattgtag aaatttttgc tttggacgga
     7141 gcttatatgc gttatacaac tatccaaaac tggtctgata acgtctataa cttggtaaca
     7201 aagcgtgcta aggctcaaaa ggatgccact gttgagtgga ttgatggaaa cttgggtgcc
     7261 aaaacgacta tgaaatatcc atctgtttac cttgatggag aaggagcgcg tggtaccatg
     7321 ctctctatcg cctttgctaa tgcagggcaa caccaagaca cgggtgctaa gatgattcac
     7381 aatgctccac ataccagctc gtctattgtg tctaaatcca tcgctaaagg tggaggaaag
     7441 gttgactacc gtggacaagt cacctttaac aagaactcta agaaatctgt ttcccacatt
     7501 gaatgtgata ccattatcat ggatgacttg tcagcatcag atactattcc atttaatgaa
     7561 attcacaact cgcaagtggc tttggaacac gaagccaaag tatctaagat ttcagaagag
     7621 caattgtatt atctcatgag ccgtggattg tcagaatctg aggcaactga aatgattgtc
     7681 atgggatttg tagaaccctt tacaaaagaa cttccaatgg aatacgcagt tgagctgaac
     7741 cgcttgatta gctatgaaat ggagggatca gttggataaa atttgatttt atactcttcg
     7801 aaaatctctt caagccacgt cagcatcgcc ttaccgtatg tatggttact gacttcgtca
     7861 gtttcatcta caacctcaaa acagtgtttt gagcaacctg cggctagctt cctagtttgt
     7921 tctttgattt tgagtattag atttactcaa aatcaaggat tttgaagatg aacttgtatc
     7981 aaaaaatcgc ggtttaaaat cgcgattttt tataatttct cgttaacaaa gcggacaaac
     8041 tgattccacc aaacttttaa gaagaaggct ttttcaattt tcttgtctgc taccatttcg
     8101 aaactagggc gctctgtggt gatgtaacct tgaccaatca agtccttgtc ttcataagtc
     8161 aaatggccaa ccactgttct agcttcaagt ggtgctggga ttgctttgga atcaggtgtg
     8221 aattgaacag attgggaaga ttgattccca atctgttcga ttagatagat atcctctgga
     8281 gccactgcag ttactgtatc ttcttttcca tcttgtacag gggctttgct atcttgatag
     8341 gcatcgcctt gttgaacgat tttgcgaagt gtaaatgtag aagaaatata atccattagg
     8401 gaagatgtag ctgtaaatcg agcgtaagga ttattgtctt gatgatctgc atttaaaaca
     8461 actgtgatga ctctcatgcc tttttcgaca gtagtaccaa caaaagactc tccagcctta
     8521 tctgttgttc ctgtttttag cccatcaaaa ccaccacggt aagcaggcat accttctaac
     8581 atgtagttgg ttgaagtgat tgtcatccca gcaaaagtag aagaaggttt tttggtgatt
     8641 tctaagactt gtgggtattt tttgatgagg ttgcgagcaa cgatagcgac atcataagca
     8701 ctaagcttat tttcctcatc ttttttagaa cctgggtaaa tgttatcccc tagagtttca
     8761 ttgttaagac ctgtcgtatt gacaacagtg gcatcctgaa ttccccattc caagagtttt
     8821 gcccgcatca tatcgacgaa atctttttct gagccagcaa ttttctcagc tagggcaata
     8881 gcggcgctgt tggcactaga taccagagtt gcttcaagca actcttcgac agtataatta
     8941 cgggcctcca taggaatatt actggcttca gaatttgtcg tcaattgata aggataatca
     9001 gaaatatcta caggagtgga gagggtaata cttccgtttt ccaaagcttc atagaccaga
     9061 taaacagtaa tcaattttgt tatggaagca atttcgacag gttgcgttgc atccttctca
     9121 tagagaattt taccagtatt tgcctcaaca gcaatcgcat gtttagcggc aatggtaaaa
     9181 tcttgagcaa cagcagtaga agcaccccct aaaagagaga cagttaacaa agttaaaaat
     9241 atttttttca tagtagtctt attctatcat aaagaaaaaa aatattcttg ctttaataat
     9301 tcatctgtta agctttttga aaatatggta aaataaagtg agggaggtaa ctcatgtttc
     9361 gtagaaataa attatttttt tggaccacag aaattttact cttaaccatc atcttttacc
     9421 tatggagaca gatggggtct ttgattaacc cttttgttag cgtgcttaat acaattatga
     9481 ttccattttt attagggggc tttctttatt atttgacaaa ccctattgtt actttcttaa
     9541 ataaagtctg taaactcaat cgtttgcttg gtattttaat taccttgtgt actttggtct
     9601 ggggaatggt cataggtgtt gtctatctct tacctatttt gattaatcag ttatctagtt
     9661 tgattatatc tagtcaaact atttatagtc gagtacaaga cttaatcata gacttatcta
     9721 attatcctgc gctccagaat ttggatgtag aagctacaat tcagcagtta aacttatcct
     9781 atgttgatat tcttcaaaat atcctaaata gcgtatcaaa tagtgtgggg agcgtcttgt
     9841 cagctcttat cagtactgtt ttgattttga ttatgactcc agtttttttg gtttatttct
     9901 tattagatgg acataaattc ttgcccatgc ttgaaagaac gattctaaag agggatcgct
     9961 tgcatattgc aggcttatta aagaatttaa atgcgacgat tgctcgctat attagtggag
    10021 tttcgattga cgcaatcatt ataggttgtt tagcttatat tggctatagt attattggtt
    10081 taaaatatgc tttagttttt gccatttttt ctggtgtagc caatttaatt ccttatgtgg
    10141 ggccaagtat tggtttgatt cctatgatca tcgcaaatat attcactgat ccccatagac
    10201 tgctgattgc agtgatttat atgcttgttg ttcagcaggt agatggcaat atcttatatc
    10261 ctcgaatcgt aggaagtgtt atgaaggttc atccaatcac gattttagtt ttacttttgt
    10321 tgtcaagcaa tatttatggt gtagttggaa tgattgtcgc agtgccaacc tattctatct
    10381 tgaaagaaat ttctaagttc ttatcccgtt tgtatgaaaa tcataaaata atgaaagaac
    10441 gagaaagaga attagctaag taaaagtcag gagaaccctg atttttcttt actggaagtg
    10501 gcctttagat tagaagactg aaaataagtt aaagtcttaa actaattttc acagctaaga
    10561 atagtagaag ttaatctgat aaaaatcgaa aaaaccagtg gaattccgtg tcagggtaag
    10621 ttccactggt tttcatagtc tattaaagtt cgaatgaaac ctatttatag tatattgaaa
    10681 ctagaatagt acacctcta
//
LOCUS       DAVKAO010000056        10637 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKAO010000056 DAVKAO010000000
VERSION     DAVKAO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.056"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            1..306
                     /locus_tag="VWX16_001563"
                     /pseudo
     CDS             1..306
                     /locus_tag="VWX16_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            404..616
                     /locus_tag="VWX16_001564"
                     /pseudo
     CDS             404..616
                     /locus_tag="VWX16_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            516..962
                     /locus_tag="VWX16_001565"
     CDS             516..962
                     /locus_tag="VWX16_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9158888.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1100..2404)
                     /gene="eno"
                     /locus_tag="VWX16_001566"
     CDS             complement(1100..2404)
                     /gene="eno"
                     /locus_tag="VWX16_001566"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9158889.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2568..3014
                     /locus_tag="VWX16_001567"
     CDS             2568..3014
                     /locus_tag="VWX16_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9158890.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3011..4126)
                     /locus_tag="VWX16_001568"
                     /pseudo
     CDS             complement(3011..4126)
                     /locus_tag="VWX16_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(4135..4464)
                     /locus_tag="VWX16_001569"
     CDS             complement(4135..4464)
                     /locus_tag="VWX16_001569"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU9158891.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4409..4780)
                     /locus_tag="VWX16_001570"
     CDS             complement(4409..4780)
                     /locus_tag="VWX16_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU9158892.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4839..6272)
                     /gene="glgA"
                     /locus_tag="VWX16_001571"
     CDS             complement(4839..6272)
                     /gene="glgA"
                     /locus_tag="VWX16_001571"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9158893.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6269..7408)
                     /gene="glgD"
                     /locus_tag="VWX16_001572"
     CDS             complement(6269..7408)
                     /gene="glgD"
                     /locus_tag="VWX16_001572"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9158894.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIKGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7398..8540)
                     /locus_tag="VWX16_001573"
     CDS             complement(7398..8540)
                     /locus_tag="VWX16_001573"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9158895.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8560..10458)
                     /gene="glgB"
                     /locus_tag="VWX16_001574"
     CDS             complement(8560..10458)
                     /gene="glgB"
                     /locus_tag="VWX16_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9158896.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTN"
BASE COUNT         3246 a         2336 c         1873 g         3182 t
ORIGIN      
        1 aatccagacg cttatttgac tgaaatagct tctgaatttg gctgtcatcc aactaccatc
       61 cactatgcac tcaaagctat gggctacact cgaaaaaaag aaccacacct actatgaaca
      121 agacccagaa aaagtagcct tatttcttaa gaattttaat agtttaaagc acctagcaca
      181 ggtctaacaa atggtgaatt aatcgctcca atgacttacg aagagatgat gacgagcgac
      241 ttttttgaag tatggtttca gaagtttctc ttaccaacat taaccacacc atcggttatt
      301 atagtgaaat gaaataagaa caggacaaat cgatcaggac agtaaaatcg aattctaaca
      361 atgttttaga agtagaggtg tactattcta gtttcaatct actatattaa tggacaatgc
      421 aagattccat agaatgggga agctagaact cttgtgtgaa gagtttgggc ataaactttt
      481 acctcttcct ccctactcgc ctgagtacaa tcttattgag aaaacatggg ctcatatcaa
      541 aaagcacctc aaaagggtat taccaagttg caataccttt tatgaggctc ttttgtcttg
      601 ttcttgtttc aattgactat ataaattgtc taagcgaaac aaccgataag aattggcaca
      661 aaagcgaccg tatttttgtt accaatacag gaaaaccagt tcatagttct atcttgagca
      721 agtctctcca gcgagcaaac gaacgcctta aaaaaccaat tcccaaacat ctgtcccctc
      781 acatcttcag acacaccact attagcatct tatcagaaaa taaaattcct ttaaaaacaa
      841 tcacggacag ggttggtcat tccgactctg aagtcactac ttccatctac actcacgtca
      901 caaagaacat gaaagatgaa gcaatcaatg tactggataa agttatgaaa aagatttttt
      961 aaaaagtttt gtcccttttt tgtcctctaa atacaaaaat agcccttcgg ataaaatccg
     1021 aggggctaga aacgttgtta aatcaacggc cgaacttttg aatttcatgg ttcgggataa
     1081 aatagttcac tgaactattt tattttttaa ggttgtagaa tgatttcaat ccacgatatt
     1141 cagctacttc accaagttgg tcttcgatac gaagcaattg gttgtattta gcgatacggt
     1201 ctgtacgtga aagtgaacca gtcttgattt gtcctgcgtt agttgcaact gcgatatcag
     1261 cgattgttga atcttcagtt tcacctgaac ggtgtgatac aacagcagtg taaccagctt
     1321 ctttagccat ttcgatagct tcaaaagttt cagtaagagt accgatttgg ttaactttga
     1381 taaggattga gttagcagca ccttcttgga taccacgtgc aaggtagtca gtgtttgtta
     1441 cgaagaagtc gtcaccaaca agttgtactt tcttaccaag acgttcagta agagctttcc
     1501 aaccatccca gtcgttttca tccataccat cttcaatagt gatgattggg tatttgttaa
     1561 ccaattcttc aaggtagtcg atttgttctg cagatgtacg aacagcagca ccttcacctt
     1621 caaatttagt gtagtcgtaa actttacgtt ctttatcgta gaattctgat gaagcacagt
     1681 caaatccgat aaatacgtct ttacctggta catatccagc agcttcaatc gcagcaagga
     1741 tagtttcaac accatcttca gttccttcga aacgaggagc gaatccacct tcgtcaccta
     1801 cggcagtttc caaaccacgt gatttaagga ttttcttaag agcgtggaag atttcagcac
     1861 cgtaacgaag ggcttcttta aatgttggcg caccaactgg caagatcatg aactcttgga
     1921 aagcgattgg agcgtcagag tgagaaccac cgttgatgat gttcatcatt ggagttggaa
     1981 gaactttagt gttgaatcca ccaaggtagc tgtaaagtgg gatttcaagg tagtcagcag
     2041 cagcacgagc tacagcgata gacacaccga ggattgcatt cgcacccaat ttacctttgt
     2101 taggagtacc gtcaagtgcg atcatagcac ggtcaatagc ttgttgatca cgtacatcgt
     2161 agccaatgat agcttcagca atgatgttgt ttacgttgtc aacagctttt tgtgtaccaa
     2221 gaccaccgta acgagatttg tcaccgtcgc gaagttcaac tgcttcgtgt tcaccagtag
     2281 aagctcctga tggaaccata ccacgtccga aagcacctga ttcagtgtaa acttctactt
     2341 caagtgttgg gttaccgcgt gagtctagga cttcgcgagc gtaaacatca gtaataattg
     2401 acatttttta ctctccttat gagttaaatt ttttacacct ctataatacc ttaaaacccc
     2461 tcctttttca agaaaaaacg ttatctttgt gcaacttttc cttaacttta taaagtaatc
     2521 gctttctttt gtctgtttta ttctaacttt tatgatatac tgttttcatg acagatttat
     2581 caaaacaatt acttgaaaaa gctcatggtg ggttaaaaat aaatccggat gagcaaagac
     2641 gctatcttgg tacttttgag gaaagagttc ttggatatgt agatattgac acagcaaata
     2701 gccctcagtt agaaaaaggc tttttattta ttttagaaaa ccttcaggaa aaagcagagc
     2761 cactatttgt gaagatttca ccaactatcg aatttgataa gcaagttttc tacttaaaag
     2821 aagcaaaaga aactgatagt caagccacca tagtatctga agagcatatt acttctcctt
     2881 ttggcctggt tattcatagc aatgcaccag ttcaagtaga agaaaaagac cttcgacttg
     2941 cttttccaaa actttgggaa gttaaaaagg aagaaccagc caaaacatcc ttatggaaga
     3001 aatggtttag ctaaatcttg cacatattta ataagtgccc aatattggca gccgtgcgct
     3061 ccagatagag actggcattt ttcaaactat cttctaaagg ttcacttttc tccaaaatag
     3121 aaaagacagc ttggatattt tcaaatggta ggggaggtaa atcttcagca agactaccgc
     3181 aaatagcaat aacaggaact ccaacagggg ttctttttgc aacacctata ggcgctttcc
     3241 cagcaaagct ttgactatca agccttcctt ctccaacaac aaccaagtca gcatctgaaa
     3301 ctttcttatt aaagttgatt aagtccaagc aggtatcaat tccagacacg atacttgcct
     3361 gagcaaaggc acacaaacca ccagcaaggc ctccacctgc tcctgctcct ttaatttcta
     3421 atgttgcagg tgagaatttt tcataaaaat cttggatcgc ctgatctacg actgcaaaca
     3481 tagtcggatg tagacctttt tgattgccaa aagtgtaagt cgcaccttga tgaccacata
     3541 agggactcac gacatctgct agaatatgaa tttgaacacc ttcaggaatt ttatagcaat
     3601 tttctgttga aacagaagct aagtttaata aggattgatc ggaagcaggc aagacatttc
     3661 catccctatc ataaaattga taacctaaac cagcagcaat ccccagtcct ccatcattac
     3721 tggccgtgcc accaacaccg atataaatat ctttaatccc tttagaaatg agatggagaa
     3781 tcaactctcc aataccacaa gtttggattt gaagtggatt tcgtttctct agcggaattt
     3841 ttccaagacc aaccaagtca gctacttcaa atagtgccag ttcccctttt tgaaaatagc
     3901 gcatggcttc tttttgtcca aaagggtctg tcacttggat ccatttttct tttaggtcaa
     3961 gagaatgtcg gatagcatct acagtacctt ctcccccatc accaacaggg cagaggagac
     4021 attctacatc tgctatcgat tattggaagc ctctttttat tgcttcagct acctgttgag
     4081 ctgtcaagct ttccttaaac gaatccggtg caattacaat cttcatattt tccctcattc
     4141 taaacagtca atcaaaggaa gaacttctaa aaaatccctc ttgtcaacat gatgtggtat
     4201 ttcttttttg agcatttctt tggaacaaaa ggcgattcct aactccgccg acttcaacat
     4261 taatagatta ttgaccccat caccgattgc caccgttctt tctttagaaa gttttagttt
     4321 ctttctccat ttttccagag tttctttttt gacctgggga cttataattt gtccaactaa
     4381 ttttcctgtt aaaagacctt ctttgacttc aagctggttg gcagtgaaat aggcaatacc
     4441 aagggatttt gctaatctct ccaactattg gtgtaaatcc accagacacc agaccaacta
     4501 ggatgccatt cttttggaga atagagatga actctgggac atttagcgat agatgaattg
     4561 agttgaagac gttatcaaag accaaaatag gaagaccttc caacaaggac actctttttc
     4621 ttaaactgct ttcaaagacc aactctcctc gcattgctcg acttgtaatc tgcgaaattt
     4681 ccgcctcatg acctgcctct ctccctaaaa gatcaatcac ttcttctagg attaaggttc
     4741 catctacatc caaaacacac aagcctttta cttgagacat cagttctcct ctctaaacag
     4801 cctaaaaatc gtatgaagtc atcatacgat tttatctatt aattaactaa actatggtac
     4861 aagtcaaggt atgacttgca ggctgtatcc catgagaagt cactctccat agcttgtttt
     4921 tgtaggtttc tccaaatgtc tggatggttt ctatacaagt ccaatgctgt ttggaaagtc
     4981 caatttaacc aataaggaga tagattgtca aagctaaagc cagtaccgct tccttcgatt
     5041 ggattgaaag cgcgaactgt atctcgcaag cctccaactt catggaccaa tggcaaggtt
     5101 ccataacgca tagccatcat ttgagacaag ccacacggtt caaaacgact tggcatgagg
     5161 aagaggtcac aagcagcgta gatttcctga gcaagtttga catcaaaagt gatatttgct
     5221 gatagcttgt ctgggtaaat ctgagcaaac catgagaaag ctccttcaaa ggctggatcg
     5281 ccagttccca aaagaacaat ctgaacatct tcttgcaaga tatggtgaag actttcgacc
     5341 accacatcaa aacctttttg acgtgtcaaa cgagaaacaa ttcccaccag tggaacgtct
     5401 gctctaacag gcaagccaac tctttcttgc aattttgcct tatttttggc tttcccagac
     5461 aaatcttcct gattgaaatg atagtctaaa agagcatccg tctgaggatt ataaagatca
     5521 gcatcaatcc cattcacgat accagatact ttaccagact ccattttaag aatctgatcc
     5581 aaattacatc caaactgact agtcataatt tcatgagcat agctaggtga aacggttgaa
     5641 acacggttcg catagagaat acctgccttc atccagttca gacagttgtt ccatcgaagg
     5701 gtgccatcag cgtaacgttc aaagccaact ccaaacaaat cacccaacat tccttctgaa
     5761 aattgtcctt ggaattctaa attatgaatg gttaaaactg tttcaatgtc ctcataggct
     5821 tgaatccaac ggtatttttc cttcaacaag aaaggaatca tagctgtatg gtagtcatga
     5881 acatggagaa gatcaggaat aaagtcaatc ctttccatag cctcaatggc agccagttgg
     5941 aaaaaggcaa agcgttctcc gtcatcaaaa tcaccgtaaa catgaccacg gaagaaataa
     6001 tattgattgt caataaagta gaaggttaca ccatttaata ctgttttctt aattccacaa
     6061 tactgtctgc gccaaccaac gctcacctca aaatgaagta catcttcaat ctgatttcca
     6121 aatttagcct ctaccatatc atagtagggt aaaatcactg caacttcgtg cccagctttt
     6181 accagtgatt ttggaagagc gccaatgacg tctcccaaac cacctgtttt tgaaaagggt
     6241 gcaccctctg ctgctacaaa taaaattttc atgaatgaat atcctctgtt actttagcac
     6301 ctttcttaac cacaactgga tgttctgcag ttcctcgaat cacaacacca ggctcaactt
     6361 caaccccttt gtccaagata gcatattcga cctgagcccc ttctccaata acaacacgag
     6421 ggaagagcag gctatcttta accaagctat ccttatggac atgaatatta cgtgatagaa
     6481 cagaattagc tacttgacct ttaataatac taccagaggc aaactgagaa gtgcttacct
     6541 tagatgtatt agcatagtaa gttggctctt cgtttttgac ctttgtataa atcttttggt
     6601 ttggtgagaa aagagaatag aatttttgtg attcaagcat atcgatattc gcttgataat
     6661 aagatttaac agagtgaata ttggctagat agcccgtgta ctcgtaggcg aaagctccct
     6721 cttttacagc caaatcccgt aaaacatagc gcaatttctt tggatgttct tttttagctt
     6781 cttcttccaa gtgttcaatc aaccaaggtg tatcaacgac aaagatatct gtagacatat
     6841 tgaacgtttc agctgttgac ttgctatcaa agagtttatg agaaagaaca tggtctgttt
     6901 catctacatc caagattgca tttacttctg aaatatcttt cttagctagt tttttataaa
     6961 ctacagtgat aggctctttt gttgtactat gtaggtggaa aacttggttc aaatcaatgt
     7021 taataagaac atcgcagttg agggcaaccg tttggtttga gccagaacgt ttcaaataag
     7081 taagaagctg ttggtagtat tcttttccaa ctgtactact ttctacacgg gtattgtaaa
     7141 ttcctagata gtaatgacta agaagggttg ataagcccca ctcgcgtcct gaacgaatat
     7201 ggtcaaatac tgagctgata ttatcctgct ggaaaatacc aaagacacta cgaacacctg
     7261 cattagcaag gcttgaaagc gggaagtcaa tcaaacgata tttcccacca aatggcaaac
     7321 ttgctactgg acggtggtcc gtcaatgtcg acatattgtg aaaaccaact gtatttccta
     7381 aaatggcaga atatttatca atcttcatct gttgctaccc ccactacttc attatatcct
     7441 acaacttgta cttcatctgt tccatcaatt tcgacaccgt cagaaataat cgcaccttca
     7501 ccaataatgg cacgtttaat cttagctcct tgaccaatga tagctccact catgataact
     7561 gaatcaagga cttccgctcc ttcgcgaact tgcgcgcctg ttgaaaggat agaatgttta
     7621 acagttccat caacgaaaca tccgtctaca actaatgagt cttccacatg agcatttgcc
     7681 ccgaggaagt ttggtggtga aatcaagttt cttgagtaaa tcttccattg acggttacga
     7741 ctatccaagg cattttctgg agaaatatac tccatgttcg cttcccaaag tgactcaata
     7801 gtaccaacat ctttccaata accactaaat tcgtaagcat aaacactttc acctgactca
     7861 aggtaatttg gaatgacatt tttaccaaag tctgacatgc caaccttgct cttttcagca
     7921 gcgactaaca tattacgaag gcgttgccaa tcaaaaatgt agattcccat agaagctttt
     7981 gtagatttag gttgagctgg tttttcttca aattcaacaa tacgattgtt agcatctgtg
     8041 ttcatgatac caaaacggct tgcttcttta agagggacgt ctaaaactgc tactgtcaag
     8101 ctggcattat tatccttatg agactggagc atatcatcat agtccatttt gtagatgtgg
     8161 tccccagaca aaatcaagac atactcagga ttgacactgt cgatatagtc gatattttgg
     8221 taaatagcgt gactagttcc ctcaaaccaa cgatttcctt cacttgcaga ataaggttga
     8281 agaatagaga cgcctgaatt aataccgtct agtccccagc ttgaaccatt cccaatatgg
     8341 ttgttgagag caagtggttg atactgtgta acgaccccaa cattatgaat ccctgagttg
     8401 gcacagtttg atagggcaaa gtcaatgata cggtagcgcc caccaaattg cacagctggt
     8461 tttgcgatgc tttgagtgag tttaccgaga cgagttcctt gcccaccagc aagaatcaaa
     8521 gctaacattt catttttcat tttctactcc tttttggttt taatttgtga cggttttagt
     8581 agatttcaag cgacgtttga ttttccatac acttgctccc atagccggta gagtaaaggt
     8641 taaggtctgc tcataatctt tccatagtcc ttcttgcgtt tgaacagttt gattatgttc
     8701 tttccaaaca cctccccact cttccaactc agtattccat acttcttcgt aaattcctgc
     8761 aacgggtagt ccgattgtaa aatctttccg ctcaacaggt accatgttaa agatacagac
     8821 taacatttct ccctttttac ccttacgaat aaaggaaaga acactctggt ctcgattatc
     8881 cgcatcaatg atttcaatac catcatagct ggtatcaatt tcccacagac agcgatgatc
     8941 tttgtaaaac tggtttagct gagaagcgaa atacttcatc ttagcattca ttgggtcttc
     9001 taggttagac cattccaact gttcttcaga tttccattct aggaattgac cgtattcgct
     9061 acccatgaag agcaatttct taccagggtg acaaatttgg tacgtataga gattgcgcaa
     9121 gcctgcaaat tgattgtaac gatctcccca catcttatgc atcatactct tcttgccatg
     9181 aaccacttca tcgtgcgaga atggcaagag ataattctcc ttgaaaacat acataaagct
     9241 gaaagtcacc aggttaaagt catatttacg ataaatcgga tcttcttcgt agaaacggag
     9301 gatatcattc atccagccca tgttccattt gtagtcaaat cctagaccac caatctcttt
     9361 cattcccgta atcttggttg cagacgaact ttcttctgca atcatcatca catctggata
     9421 ttctaactta ataacttcat tcaagcgttg aagaaaataa taaccttcat agttgagatt
     9481 tccgccatct ttattaggtg tccatggagc atcatcatag tccaaataga gcatgttgct
     9541 aacagcatcc acacgaatac catccaaatg atagacatca atccaatgct taatgcaaga
     9601 aattaagaag gactggactt cattttttcc aaggtcaaaa ttaagggcac cccaaccatg
     9661 gttatgagcc ttattatggt cttggtattc aaaagtcggt gtcccatcat aataggctaa
     9721 ggcatcatcg ttgatggtaa agtgacctgg tacccagtcc acaataaccc caatattatg
     9781 ggtatgacac tcctcgacaa aatcttgaaa ctcctctggt ctgccataag catgctctaa
     9841 agcgaagtaa cccataagct gataccccca actcaagccc aaaggatggg acatcaaggg
     9901 cataaactca atatgagtat agttcatttc aacgagataa ggaatgagtt catccttgag
     9961 ctgggcaaaa ctataaggac tgccatcaga atttcttttc catgatccag cgtgaacttc
    10021 ataaatattg acaggacgct cttcaaagcc ccaacgtttt cttcgtgcca gccaaagtcc
    10081 atccttccat ttcttctcag gaagctctgt taagattgcc cctgttcctg gacgagcctc
    10141 atacctgaca gcaaaagggt caatcttcat cagttgatga ccattttgac gtgtgacatg
    10201 atatttgtaa atatgccctt cttgagccat attggtaaag acttcccaga ccccaaagtc
    10261 atttcttacc attggaatct gattttcaat ccaattggta aaatcaccaa ccaagtgaac
    10321 agcctgagca ttaggtgccc aaacacggaa ggtatagcca tgctctccat ttagttcttc
    10381 cctatgtgct cctagataat gttggagata aaaattttca cccgtcataa aggtttttaa
    10441 tgcttctcta ttatccatat actccccttc tcctgtaagc gttttctatg tttttattat
    10501 actacctttt tagagaagat tcaagtaaat tactatactt ctttaattat tttgaaaatc
    10561 tacaacaagt tcacttactc gttcaattgt aaatcaatat tttttcaaaa aattgcgaaa
    10621 acgcctttct ttttcta
//
LOCUS       DAVKAO010000057        10621 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKAO010000057 DAVKAO010000000
VERSION     DAVKAO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.057"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            152..271
                     /locus_tag="VWX16_001575"
     CDS             152..271
                     /locus_tag="VWX16_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9158897.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            1057..1440
                     /locus_tag="VWX16_001576"
     CDS             1057..1440
                     /locus_tag="VWX16_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9158898.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            1492..2181
                     /locus_tag="VWX16_001577"
     CDS             1492..2181
                     /locus_tag="VWX16_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9158899.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVAIFP
                     FLLTFLHRVLG"
     gene            2223..2471
                     /locus_tag="VWX16_001578"
     CDS             2223..2471
                     /locus_tag="VWX16_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158900.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLDLKNDV"
     gene            2501..3112
                     /locus_tag="VWX16_001579"
     CDS             2501..3112
                     /locus_tag="VWX16_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9158901.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGVFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            3273..4067
                     /locus_tag="VWX16_001580"
     CDS             3273..4067
                     /locus_tag="VWX16_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9158902.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            4064..4699
                     /gene="trmB"
                     /locus_tag="VWX16_001581"
     CDS             4064..4699
                     /gene="trmB"
                     /locus_tag="VWX16_001581"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9158903.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIGRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4825..5304
                     /gene="rimP"
                     /locus_tag="VWX16_001582"
     CDS             4825..5304
                     /gene="rimP"
                     /locus_tag="VWX16_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9158904.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            5348..6484
                     /gene="nusA"
                     /locus_tag="VWX16_001583"
     CDS             5348..6484
                     /gene="nusA"
                     /locus_tag="VWX16_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9158905.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            6506..6799
                     /locus_tag="VWX16_001584"
     CDS             6506..6799
                     /locus_tag="VWX16_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9158906.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKRVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6792..7091
                     /locus_tag="VWX16_001585"
     CDS             6792..7091
                     /locus_tag="VWX16_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9158907.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            7108..9900
                     /gene="infB"
                     /locus_tag="VWX16_001586"
     CDS             7108..9900
                     /gene="infB"
                     /locus_tag="VWX16_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9158908.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            10150..10500
                     /gene="rbfA"
                     /locus_tag="VWX16_001587"
     CDS             10150..10500
                     /gene="rbfA"
                     /locus_tag="VWX16_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9158909.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3240 a         1903 c         2411 g         3067 t
ORIGIN      
        1 gcaggggttg catatggcgc aattgatggg tgtgcaacaa cggtttgata tttctattgg
       61 gaccatttgc tataggaata ggtgtaactg gtgctgcagg tggagctata cttggaggtg
      121 tagcctatgc agcaatatgt tggtggtaat tatggatttt aaaagtttta ttattggttt
      181 agtagttggt atatttggtc cttatatgga tgacttaatt agaaaaatat tttcaaaatc
      241 ttccaaaaag gatacggata gtactctcta aaaaataaat ttctcccctg tatttgctag
      301 cttaatttac aggggagttt ctttaatatt gtttcgcctg tcaaaatgat atgtgatagc
      361 aggatgaaaa gtctacaaac aagttggtat caatacaata taaagaggaa agtatgtttt
      421 taatgcttac tttttaaatt ttttaacaat tcaagatgtt tcgatgacaa ttcaagattt
      481 ggatgaaaaa ttttaaaaaa caatgatata ctaaacttgt caaagttgca acgggtgctg
      541 ctacattatg ttaaaaatcg ctcttgattg ataagctgga tttgttagga aaggagtaaa
      601 taattatgga taagaaaaag atagtttcga ctataatatg tatagtattt cttgtagtgt
      661 cagtagataa tttttttaga gacttaaccc cgttactgtt cattttaaat attattggcc
      721 tgtcttgctt ttcagtgcta acatacatta acataaaaga gatactctta aatataagta
      781 aatgatgatt tggttagtcc tagaaccaat caacttatgt ttatcggctt ggcagatttt
      841 atgtttgtca tttgtttgta tagaggtatt tcagaaacag aattctatca acaactaatc
      901 gcttatatag gtgtattctc tgcttgttta tcacgcttct gctcatgtgg ggcttgaagt
      961 attataaaaa gtagctttct atccttttct gacaggatat ttgagtagaa ggctctatta
     1021 aattgttagt gaaataaatg aatggaggta tttaatatga aatacagatt attttttgtt
     1081 attttcttga gtagtgtgtt ggatattctt ttagggacat ttttacaaat ctctatcgta
     1141 tctattgggt ggcttgttct ttacagtggt ttgtttgaag caggagtttt ccttcttgct
     1201 aataaagggg tggcggtaaa aatcaaggaa gtagatattc gaaatcgctt taaatttatt
     1261 tttggaaaaa ccttatggtt tcaaattctt ttgctcatct ttttgataat caaactttat
     1321 cttggtttgg atgcgaggtt gattttattc tatggacata ttttcattgt ctttaatgcc
     1381 ttaatgtatc tattatctag tagtcaggtt agccttaaaa aaaacaaact gtcttcttaa
     1441 tcttacagca gtagagcctg acaaggtgag gtcatagtag ggaggatttc tatgaaaaag
     1501 tatcaacttc tattcaaaat aagtgcagtc ttctcttact tatttttcgt atttggtctt
     1561 tctcagctga cgcttatcgt ccaaaactat tggcaatttt cttctcagat aggcaattta
     1621 ttctggattc aaaatatctt gagtttactt tttattggag tcatgattgt ggttcttgtt
     1681 aagacaggcc atggttatct cttccgcatt ccaagaaaaa aatggctttg gtattcgatt
     1741 ttgacagtat tagtggtagt gttccagatc tcttttaacg ttcagacagc taaacatgtt
     1801 cagtcaactg cggaaggttg ggctgtattg attggttata gtgggactaa ctttgcagag
     1861 ctaggtattt atatagccct gttctttctg gttccactga tggaagaatt gatttataga
     1921 ggattactgc aacatgcttt ctttaagcat tcgcgatttg gtcttgattt gcttcttcct
     1981 tctattttat ttgctctccc tcatttttca agcctgccta gtctgttaga tatcttcgtc
     2041 tttgcaacag ttggaatcat ctttgctggt ttgacccgct ataccaagag catttatcca
     2101 tcctatgcgg tgcatgtgat caataatatt gtagcgatct tcccgttttt gctcactttt
     2161 ctacataggg tcttggggta aaaaaacaaa aggcttgctt ttcagccata gaggaggtca
     2221 tcatgtataa acacttattt ttcctagatt ccaaaacttt agatcggttg acaccctata
     2281 ttctagtctt ggcttctgac accattgctt ttaatgtttt tgtgctaacc tttgtatctg
     2341 cggtggtttt taatttccta aattccatgc tagctttaat ggctatattc ataggggctg
     2401 gctatgtggt cggattttgg ttgctaaaat ggtttgtttt ggaaaggcta gatctaaaga
     2461 atgacgtgta ggaagtctgt ttgttgagga ggataatttt atggagtttt ttgataaatt
     2521 tcatgccttg tgttttggat ttttagtact aataattgtc attacagttc cttatacgat
     2581 taaccatggg ggggtttttc aaaatgaatc tgcattgatt cttgtaagtc ttcttgtaac
     2641 ctcgctgagt gttgcttatg ctagaaagtt tgaaatgatt tcttttggga tgttaagcaa
     2701 gaaacaactt ttgcttttca ttgcaatctt tcttctaagt gtacttgaga cgctggttta
     2761 tattcatttc ttcgctgttt cttctggctc aggggtccaa cacttggcgg aagtcagcag
     2821 aggaatttcc ctgtctttga ttttaactac ctcagttttt ggccccatcc aggaggaact
     2881 cattttcaga ggacttcttc aaggtgcggt ttttgacaat tcttggttag ggcttgtgct
     2941 aacttcctct ctcttttctt tcatgcatgg accttctaat gtcccttcgt ttatttttta
     3001 tctacttggg ggtttgttgc tgggctttgc ttataaaaag agtcaaaacc tatgggtttc
     3061 tactctagtc catatgcttt acaacagttg gccactctta tattatttat aaaaattatg
     3121 aaaaggtaag tagaagacgg tgcttacctt tttcaagcta taatgatacc caagccaatc
     3181 cagaggcttt tctttgagaa tcgggttaga gtggttgacg aataggccaa aaactagtag
     3241 aatagtaagg aaactttata cggaggaaag aaatggattt gggtgataat gagctaacac
     3301 tgactcccat acctgggaaa agtggcaagg cttatatggg tagctatcct gatgggaagc
     3361 gcatctttgt aaaaatgaac acctctccaa tcctacctgg tctagctaga gaacaaattg
     3421 ctccacaatt attatggagt cgtcgtttgg cagatgggcg tgatatgtgt gctcaagaat
     3481 ggttgacagg caagatattg accccctatg atatgaatcg taagcaaatc gtcaatattt
     3541 taacccgtct tcatcgctca cgtccgttga tgacacaatt gagtcgtttg ggctatgcca
     3601 tggaaacacc tgtagattta ctacagtctt ggcaggaaac ggctccagat gctttgcgta
     3661 aaaatcattt tatcagtgaa gtgatggctg atttacgtca gactattcca ggatttagag
     3721 aggaccatgc gaccattgtc catggagatg tacgacatag taattggatt gagacagata
     3781 gtggcttgat ttatttagta gattgggatt cggttcgctt gaccgatcgc atgtttgatg
     3841 tggcccatat gctctgccat tatatttcag aacatcagtg gaaggaatgg ttgacctact
     3901 acggttacaa gtacaatcaa acggtattaa gtaaattgta ttggtatggt caattgtctt
     3961 atttgagtca gatttccaag tattatatga accaagattt agaaaatgtc aatcgggaga
     4021 ttcatggttt gcgtcatttc cgagacaagt atggaaagag aagatgagag ttagaaatcg
     4081 taaaggggca acagaattac tagaggcaaa tccccagtat gtggtcctca atcccttgga
     4141 agccaaggca aaatggcggg acttgtttgg caatgataat cccattcatg tggaagttgg
     4201 aagtggaaag ggtgcctttg tttcaggtat ggccaagcaa aaccctgaca tcaactatat
     4261 cgggattgat attcaaaagt ctgttttgag ctacgctttg gacaaggtgc ttgaagctgg
     4321 agtgtctaac atcaagctct tgtgggtaga tggttctgac ttgactgact actttgaaga
     4381 cggtgagatt ggtcgcttgt atctgaactt ttcagatcca tggccgaaaa aacgccatga
     4441 aaagcgtcgt ttgacctaca agaccttttt ggataccttc aaacgtatct tgcctgaaaa
     4501 tggagaaatt catttcaaga cggataaccg tggcttgttt gagtacagtt tagtgagctt
     4561 ttctcaatat ggcatgaaac tcaatggtgt ctggctagat ttgcatgcca gtgattttga
     4621 aggcaatgtc atgacagaat acgagcaaaa attctcaaac aaggggcaag ttatctaccg
     4681 agttgaggca gaattttaag agatagccta aaattaggct gtacaagtgc ttttgcttta
     4741 cataagttgg caaacgtgct atactgatag taagaatatg aaaagtgagg cggggaaata
     4801 tcttcgcctc ttgcttatga ggaggtggac gcaatcgcaa caatcgtaga attagtcaga
     4861 gaagttgtag aacctgtcat agaagctcct tttgaactcg tggatatcga gtatggaaag
     4921 attggcagtg acatgattct cagtattttt gtagataaac ccgaaggaat taccttgaac
     4981 gacacggcag acttgacaga aatgatcagt cctgtcctag acaccatcaa gccagatccc
     5041 ttcccagaac aatatttcct agaaattacc agtccaggtt tggaacgccc tttgaaaacc
     5101 aaggatgccg tcgctggagc ggttggaaaa tacatccatg tcgggctcta ccaagccatc
     5161 gataagcaaa aggtctttga aggaaccttg ttggccttcg aagaggacga gttgactatg
     5221 gaatatatgg acaagacgcg taagaaaacc gtccaaattc catacagttt agtatcaaaa
     5281 gcacgtttag cagttaaatt atagaaaaag aaaggatagc ttttgaggat tcaaaagtga
     5341 agaaaacatg agtaaagaaa tgctagaggc cttccgcatt ttggaagaag acaagggaat
     5401 caaaaaagaa gatatcatcg acgcagtagt agagtcgctt cgttccgctt atcgcagacg
     5461 ctatggtcag tcagacagcg tagctattga cttcaacgaa aaaacaggtg actttacagt
     5521 ttatactgtc cgtgaagttg ttgatgaagt atttgatagc cgtttggaaa tcagcttgaa
     5581 agatgctctt gccattaatt cagcttatga acttggagac aaaatcaagt ttgaagaagc
     5641 accagctgag tttggtcgtg tagcagccca atctgccaaa caaaccatca tggaaaaaat
     5701 gcgcaagcaa acacgtgcca tcacttacaa tacttacaaa gaacatgagc aagaaatcat
     5761 gtctggtaca gtagaacgct ttgacaaccg ctttatctat gtcaaccttg gtagcatcga
     5821 agcccaattg tcaaaacaag accaaattcc tggagaagtt tttgcttctc atgatcgtat
     5881 cgaagtttat gtttacaagg ttgaagacaa ccctcgtggt gtgaacgtct ttgttagccg
     5941 tagccatcca gaaatgatca aacgtttgat ggagcaagaa attccagaag tttatgatgg
     6001 aactgttgaa atcatgagcg tggctcgtga agcaggtgac cgtacgaagg ttgctgttcg
     6061 tagccacaat ccaaacgtgg atgctatcgg tacaatcgtt ggacgtggtg gtgctaatat
     6121 caagaagatt actagcaaat tccacccagc tcgttacgat gctaaaaatg accgcatggt
     6181 accaatcgaa gaaaatatcg atgttatcga gtgggtagca gatccagctg aatttatcta
     6241 caatgccatc gctcctgctg aggttgacca agttatcttt gatgaaaacg acagcaaacg
     6301 tgccttggtg gttgttccag ataacaagct ttctcttgcc attggtcgtc gtggacaaaa
     6361 cgtgcgcttg gcggctcact tgactggtta ccgtatcgat atcaagtctg ctagcgaatt
     6421 tgaagccatg gaagacgctg cttcagtaga gttggaagta gaaaacgata ctgtagaaga
     6481 ataaaagctg ctagaggagg gaaagatgaa aacaagaaaa atccctttgc gcaagtctgt
     6541 tgtgtctaac gaagtgattg ataagcgtga tttgctccgc attgtcaaga acaaggaagg
     6601 acaagtcttt attgatccta cgggcaaggc caatggccgc ggcgcttata tcaaactaga
     6661 caatgcagaa gccctagagg cgaaaaagaa gagggtcttt aaccgcagct ttagcatgga
     6721 agtggaagaa agcttttatg acgagttgat cgcttatgtg gatcacaaag tgaaaagaag
     6781 agagttggga cttgaataag caaaagataa gtaatctctt ggggcttgct cagcgagcag
     6841 ggcgcatcat atcgggtgaa gaattggtgg tcaaggccat tcaagacggc aaggccaagt
     6901 tggtctttct agctcatgat gctggaccca atctgaccaa gaagattcaa gataaaagtc
     6961 attattatca agtagaaatt gtaaccgtgt tttcaacact ggaattaagc atagcagtcg
     7021 ggaaatcgag aaaggttttg gctgtaacag atgctggatt tacaaagaaa atgaggtctc
     7081 ttatggaata gaagaggagg acatgatttg tctaagaaaa gattgtacga aatcgcaaaa
     7141 gaacttggaa aagaaagtaa agaagttgta gcgcgtgcaa aagagttagg cttggatgtg
     7201 aaaagccact catcaagtgt ggaagaagct gtcgctgcaa aaattgctgc cagctttaag
     7261 cctgcagctg ctccgaaagt agaagcaaaa cctgcagcac caaaagtaag tgcagaaaag
     7321 aaagccgaaa aatctgagcc agctaaacca gctgtagcta aggaagaggc aaaaccggct
     7381 gagccagttg ctccgaaaac agaaaaagta gcagtgaaac cgcaaagtcg taatttcaag
     7441 gctgagcgtg aagcccgtgc taaagagcag gcagaacgac gcaagcaaaa taagggcaat
     7501 aaccgtgacc aacaacaaaa cggaaaccgt cagaaaaacg acggccgtaa tggtggaaaa
     7561 caaggtcaaa gcaaccgcga caatcgtcgc tttaatgacc aagctaagaa gcagcaaggt
     7621 cagcaaaaac gtagaaatga gcgacgtcag caagaggata aacgttcaaa tcaagcggct
     7681 ccacgtattg actttaaagc ccgtgcagca gccctaaaag cagagcaaaa tgcagagtac
     7741 gctcgttcaa gtgaggaacg cttcaagcag tatcaggctg ctaaagaagc cttggctcaa
     7801 gctaacaaac gcaaggaacc agaggaaatc tttgaagaag cggctaagtt agctgaacaa
     7861 gcacagcaag ttcaagcagt ggttgaagtc gtccctgaga aaaaagaacc tgcagtggat
     7921 acacgtcgta aaaaacaagc tcgaccagac aaaaatcgtg acgattatga tcatgaagaa
     7981 gatggtccta gaaaacaaca aaagaatcga agtagtcaaa atcaagtgag aaatcaaaag
     8041 aatagtaact ggaataacaa caaaaagaac aaaaaaggca ataacaagaa caaccgtaat
     8101 cagactccaa aacctgttac ggagcgtaaa ttccatgaat tgccaacaga atttgaatat
     8161 acagatggta tgaccgttgc ggaaatcgca aaacgtatca aacgtgaacc agctgaaatc
     8221 gttaagaaac ttttcatgat gggtgtcatg gccacacaaa accaatcctt ggatggggaa
     8281 acaattgaac tcctcatggt ggattacggt atcgaagcca aacaaaaggt tgaagtggat
     8341 aatgctgaca tcgaacgttt ctttgtcgaa gatggttatc tcaatgaaga tgaattggtt
     8401 gagcgtccac cagttgttac tatcatggga cacgttgacc acggtaaaac aacccttttg
     8461 gatactcttc gtaactcacg tgttgcgaca ggtgaagcag gtggtattac tcagcatatc
     8521 ggtgcctacc aaatcgtgga aaatggtaag aagattacct tccttgatac accaggacac
     8581 gcggccttta catcaatgcg tgcgcgtggt gcttctgtta ccgatattac gatcttggtc
     8641 gtagcggcag atgacggggt tatgcctcag actattgaag ccatcaacca ctcaaaagca
     8701 gctaacgttc caatcatcgt agccattaac aagattgata aaccaggtgc taacccagaa
     8761 cgcgttatcg gtgaattggc agagcatggt gtgatgtcaa ccgcttgggg tggagattct
     8821 gaatttgttg aaatctcagc taaattcaac caaaatatcg aagaattgtt ggaaacagtc
     8881 cttcttgtag ctgaaatcca agaactcaag gcagacccaa cagtgcgtgc aatcggtacg
     8941 gttatcgaag cgcgcttgga taaaggaaaa ggtgcggtcg caacccttct tgtacaacaa
     9001 ggtaccttga atgttcaaga cccaatcgtt gtcggaaata ccttcggtcg tgtccgtgct
     9061 atgaccaacg accttggtcg tcgtgttaaa gttgctggac catcaacacc agtctctatc
     9121 acaggtttga acgaagcacc gatggcgggt gaccactttg ccgtttacga ggatgaaaaa
     9181 tctgcgcgtg cagcaggtga agagcgtgcc aaacgtgccc ttatgaaaca acgtcaagct
     9241 acccaacgtg ttagccttga aaacctcttt gataccctta aagctgggga actcaaatct
     9301 gttaatgtta tcatcaaggc tgatgtacaa ggttctgttg aagccctttc tgcctcactt
     9361 caaaagattg acgtggaagg tgtcaaagtg actatcgtcc actcagcggt cggtgctatc
     9421 aacgaatcag acgtgaccct tgccgaagct tcaaatgcct ttatcgttgg tttcaacgta
     9481 cgccctacac cacaagctcg tcaacaagca gaagctgacg atgtggaaat ccgtcttcac
     9541 agcattatct acaaggttat cgaagagatg gaagaagcta tgaaagggat gcttgatcca
     9601 gaatttgaag aaaaagttat tggtgaagcg gttatccgtg aaaccttcaa ggtgtctaaa
     9661 gtgggaacta tcggtggatt tatggttatc aacggtaagg ttgcccgtga ctctaaagtc
     9721 cgtgttatcc gtgatggtgt cgttatctat gatggtgaac tcgcaagctt gaaacactat
     9781 aaagacgacg ttaaggaagt tacaaacggc cgtgaaggtg gattgatgat cgacggctac
     9841 aatgatatta agatggatga tgtgattgag gcgtatgtca tggaagaaat caagagataa
     9901 gattttttgc tcctttctta ggtggtgagg gacgcaagca aaccgatgtt tcattgctta
     9961 tttttgagcc tagggtctca aaaatcccct gtgatgggac tgataaatca gttccatcac
    10021 tttcaccacg gcgaaagaag cagatgactt caaattgaac ttcgtttcaa tttaaactga
    10081 aaatcaagaa gtttaaaata gctaggtctg ctggcctagc ttttggttca aagtagagaa
    10141 aggaatatca tggcaaatca tttccgtacg gatcgtgtgg gcatggaaat caagcgtgaa
    10201 gtcaatgaga ttttgcaaaa gaaagtccgt gatccacgtg tccaaggtgt gaccatcaca
    10261 gatgttcaga tgctgggtga cttgtctgtt gccaaggttt attacaccat tttgagtaac
    10321 cttgcttcgg ataaccaaaa agcccaaata gggcttgaaa aagcaactgg taccatcaaa
    10381 cgtgaacttg gtcgcaattt gaaattgtat aaaatcccag atttgacctt cgtcaaagac
    10441 gagtccatcg agtatggaaa caagattgac gagatgctac gcaatctgga taagaactaa
    10501 agaagagggg tgcccctctt ttttggtgga ggaaaatagg ttgaatttga aatggaaaaa
    10561 tattctttta aaatatattg aaactagaat agtacgcctc tacttctaaa acattgttag
    10621 a
//
LOCUS       DAVKAO010000058        10601 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKAO010000058 DAVKAO010000000
VERSION     DAVKAO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.058"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(92..1516)
                     /locus_tag="VWX16_001588"
     CDS             complement(92..1516)
                     /locus_tag="VWX16_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9158910.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTATILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2061..4340)
                     /gene="pulA"
                     /locus_tag="VWX16_001589"
     CDS             complement(2061..4340)
                     /gene="pulA"
                     /locus_tag="VWX16_001589"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9158911.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLTPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGTYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTVAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4452..6410)
                     /gene="ligA"
                     /locus_tag="VWX16_001590"
     CDS             complement(4452..6410)
                     /gene="ligA"
                     /locus_tag="VWX16_001590"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9158912.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDKIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6503..7681)
                     /locus_tag="VWX16_001591"
     CDS             complement(6503..7681)
                     /locus_tag="VWX16_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9158913.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSIIVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FFLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7761..8624)
                     /locus_tag="VWX16_001592"
     CDS             complement(7761..8624)
                     /locus_tag="VWX16_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9158914.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQETMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8627..10528)
                     /locus_tag="VWX16_001593"
     CDS             complement(8627..10528)
                     /locus_tag="VWX16_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9158915.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAKIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3184 a         2336 c         1900 g         3181 t
ORIGIN      
        1 attataatac caaaaagttc gtattttttc tataattatt attttatagc aaaaaaataa
       61 aaccaggaaa acaatttcct ggttttacac gttatttcac atcaaaaaca atggatttga
      121 catttgtcat cgcttcaatg ctatatttaa ttccttgcac tccagctcca gaacctttga
      181 caccaaggaa tgggaaatta tctggaccac gctgggtttt attattaatg tggactgtac
      241 ctacttcaag cttttcagca atttcaaagg cttttttgaa atcatttgta aagactgatg
      301 attgaaggcc gaattcagat tcgttggcaa aggcaatagc ttcctctaca ctagccacac
      361 gaatgattgg taaaacagga ccaaatggct cttcccatgc cactttcata tcttttgtaa
      421 cttggtcaaa aagcactggc cagaggagat tgccctcacg tttgattggt gtaagagcct
      481 gagcctcttt ttcttgtgca tcctcaatca agccccaaat gaagtcggct gaagcattgt
      541 caataacagg tgtaatatca gcattgtcaa atggatcacc aactgttaat ttagaaactt
      601 cttcctgaag caaagtagct aatttatctg ctacactttc gagaacaatg acacgtttaa
      661 tggccgtgca acgttgtcct gagtagctaa aggctcccgc aacaatttgc ttggcagcat
      721 gttccaaatc tgcatcttct agtacaagag ctgcatcttt cccaccaagt tccaacatga
      781 taggacgcat accagctaaa cgaccaatac gttctccaat aggagttgaa cctgtaaagt
      841 tgatgaagtt gacttctttg tgctcaatga tataatcccc aatttctgaa ccacgacctg
      901 taatggtgtt gaaaacacct gccggaatcc ctgcttcttc aaatgcttta gccaacaaga
      961 gtccagaaat ggaaccttgt gttggtggct tcaacatgac cacattccct gcaatcaagg
     1021 caggtgcaat tttagaagca gataaattaa ctggataatt aaagggagca atcgctagca
     1081 cgataccaac tggttcacga cggacaacag ccagtttgtt tttacttgct gcctcaaaac
     1141 caccaccttc cattgcttgt ccagtgatac ggagaccttc ctcagcagca taacgaatca
     1201 agtctgctgt acgcactact tctccaattg ctgctttaat cccttttgct acttctttgg
     1261 caaggatagt accaattttt tccttatcgc gttctaaaat agttgctgtt ttatgcaaat
     1321 aagccgcacg ttcaattgct gataaagctc gccatgctgg tagggctgca cgcgcagctt
     1381 gcatagcctc atcagcttca gtctgagtca tggctggaac tgtacccaat tcttcttgat
     1441 tgattggtga ataaatcgta atttcttgtt cagatgattt ccattttcca tttactaaat
     1501 tctgatatct tgtcaaattc ttgtcctcct gaaaatgatt aagatatatt ttaccaaatt
     1561 ttcagaaaat cacaaccctt tttgcaaaat ttttgagatt attttcacaa gcttgatttt
     1621 tcaaagtata ctcaataaaa ataaaaaaaa tccactacat caaggcgaga ctaatgtggt
     1681 ttgaagaaat tttcgaagag cgtgaatgag tatcatctat agtaaaataa aaaaactgaa
     1741 caatttggtt ggggacagtc aaaccaattt ctcacaatgt tttagaaaca agggtgtgct
     1801 attccaattt caggctacta taactgtcat agattgctga aacaaagtct aggtaaaagt
     1861 cttcataata aaaagacccc ctatcaagtg ttcaaaaact ttgataggag gtcttgtttt
     1921 gtgaaaatat ttatcaaatt ttctatacaa gtgagctgtt agccaggttc tttctattct
     1981 ttcaatttca atgaatggat tttttaccaa tactcataac tgggaatttg tctgtctaaa
     2041 aatagcgaga tagatggtat ttataaaaca ctcaagacag ctagactaat atcatttaaa
     2101 acattatctt cttttgagcg actgttggtt accaacatag ctaaatttcc tgcattttca
     2161 aattgatagg gttctgattt agcattcaca actaccaaga ggtgttcttt gccgtgaact
     2221 tcatagataa ggcagccgct atgttcaatc gcagaatgca caaagacatg atggtaaatt
     2281 tcatcatagc tagagtaaga aaaagcaaca gtttttgtct tcaatcggat gacttgacgg
     2341 ataaactcaa tactgtcttg acgctcatta atcaagttcc agttcacttg gttcacactg
     2401 tcaggagcat tatagctatt catcgcacgc tctctatcat catgggtcaa ctcaccattt
     2461 tcaccagtcg caaccagttt ggtacgacca aattcttgac cgatttccat aaaggccatc
     2521 ccctgcatga gcagattcat ggctgtggca gtttcgacct tgcgcatgat ttgctctgaa
     2581 ctttggtctg gatgaagggt tgccaataaa tcgtgaagat tgtaattgtc atgggcttct
     2641 acatagttaa gcacctgatt tggatgtgta taggttccta attcacgact tcctaggatt
     2701 gctttagcta gaattggctc tgtcgcagca ccactgacaa aacctgactt gatagcacca
     2761 taaacttctc cccctttgac agcatcgcgc tgattgtcat taaagaaacc aatatttggc
     2821 atctggtagg cattgtcctt cttggcctta tcataagggg taagacctgt tcccatatcc
     2881 catccttctc catagaggat aatgttggag tcgatttcat ccaagctttg acgaatcatc
     2941 tgcatggtct tgacatcatg aatccccatc aagtcaaaac ggaagccgtc aatattatat
     3001 tcctgcaccc agtatagaag agaatcaatc atatacttgc gaaacatttc gtgttcactg
     3061 gctgtttcat ttccaacacc cgttccattc tggaaggtac catctggatt catacgataa
     3121 tagtaatcag ggactgttgt ttggaatggt gcatcaacaa ctgagaaggt atggttatag
     3181 actacatcca taatgactcc aatacccgca tcgtgataag cttgaaccat caccttcaaa
     3241 tcacgaatga cctgagctgg atcatctgga ttagttgaaa aactagtttc tggcgcgtta
     3301 tagttttgtg gatcataacc ccagttgtag gttacatttc catcctcatc gtattcttta
     3361 tgacggtctg caattggttg caattgaaca taattgtagc ccagcttctt gatgtaatca
     3421 aaagcagttg actggccgta ttggttaact gttccagcct gagcagcacc caagaaagtt
     3481 cctcgaagat gttcatctac acccgatgta ggtgatttag tcaaatcacg aatgtgcatt
     3541 tcacagataa ctgccttaca tggattttcc aagcgccaag tagcctccga accgtgctta
     3601 acctcgaagt tttcaacttg cttttctaca tggctcagaa tagctgaacg tttgccatca
     3661 gggctggtcg cgattgtata aggatcacgt gtcagtgttt ggtgatgagg gaattggact
     3721 tgatactgat aagtcttacc taccaaatct tcttcaacat ccaaactcca gacaccgatt
     3781 gtattgtcct tatgattata agagtagcta ttgcctcttt tcatctcaaa agtcttccaa
     3841 acgggtgcat cattagcagc tgattcataa acgacaactt gcacttctgt cgctgtaggt
     3901 gaccagaggg aaaaatgagc ctgattgtcc tctacacggc aacccaattc tccttggtaa
     3961 ccccaatgat gatcaaaact agcactgtta atggccttat caaaggcaaa aggattttga
     4021 tttttataga aaggactggc aatagcagga ttttcagagt aataaatcct atcatcgcct
     4081 tccaaaatcc agacctctgt taatagggga tagtgattaa aacggataga atattcttta
     4141 ctagtttgac ctgtatgaac cacaaaattc aagctttcta taacatgtga acttgggtgt
     4201 tcaaagctaa ataaagctcc aaaataatct tctttgtagg ttagcaaatc aattcgttga
     4261 tcctgacttt ttacaaagga gcaagtgtca tattctccat tcttacgatg gtaatgaatg
     4321 cgcatagggt agttatacat tttttatttt tcctttttac tttgtttcta tttcactaat
     4381 aaatttttgt caatctcgtc tcaattaaca gacatagtca tattctctaa actctgtttt
     4441 taaacgatcc attacaaact ttctagccat gcctcatctc tgacctggat accaagttct
     4501 tgtgcttttt gcagtttact tccagcgtct gcacctacca cgacgaggtc ggtcttttta
     4561 gaaacactac ctgtcacttt ggcacccaga ctttcgagtt tacttttagc ttctgagcgc
     4621 ttgagtcgtt ccaattttcc tgtcaatacc acggtcaaac ctgacaaggc cgcatccgct
     4681 actaccgtct gtcctttata gtccagattg accccagttt ctttcaattc tctgagcaga
     4741 atttcagagc cttctgtcgc aaaataagtc tgaagacttt tggcaatcac gccacctaga
     4801 ctttcaatac tagccacttc ctctgaatct gcctgataca gattttcaat tgaatggaaa
     4861 tattgaagta aaagctgact agccttgctt ccgacatgac gaattcccaa accaaataag
     4921 agcttctcgg cagaattttc ctttgatgct tggatagcct gatacagttt agcagcggac
     4981 ttttccttaa ccccctctaa aaggaggaaa tcctcttctt gcaaacgata aatatccgcc
     5041 acatccttga ctaaattagc agcaaaaagc ttctcaacaa tagatggacc aaggcctgta
     5101 atattcatag catcacgaga agcaaagtga atcaagcctt ccatgatttg agcagggcaa
     5161 cgcggattga tacaacgtag ggccacttca tcttcaaagt gcaacaagtc agagttacaa
     5221 cttggacagt ttgtagggat atctagtttt tcttcagaaa cccgtttgga ctctaccaca
     5281 cgtaaaacgg cagggatgat gtcaccagcc ttatatacaa tgaccgtatc gtcttttcgg
     5341 atatcttttt cagcaatata atctacattg tgcagggtcg cacggctaac agtcgtaccg
     5401 gcaagttgta ctggtgttag attagcagtt ggagttacaa caccggtacg gccaactgtc
     5461 cagtcaactg ataagagttg agcttctttt tcttcagcag ggaacttgta ggctactgcc
     5521 cactttggag ccttaacggt aaaaccaagt tcttcttgac ttgctaggtc gttgaccttg
     5581 attaccactc catcaatatc gtaaggcaga ttttcccgtt cctgtcctac ttcttggata
     5641 aaattccaga ttttatctat gttttcagcc aagattcgct taggattgac cacaaaacct
     5701 agttgttcta ggtacttcaa acccttttct tggctatcac gagttgaagg gctggcttct
     5761 tgatagagaa acgttgcaag attacgcttg gcaactactg ctgtatccaa ctgacgcaga
     5821 gttcctgctg ccgcattacg aggattagca aattcaggct ctccattttc ttggcgcact
     5881 tggttaactt ggtcaaagga agcgcgtggc atgtaacatt ccccacgaac tgtgatatct
     5941 agttcttctg gcaaagtcaa agggatgtcc ttaacacgct tgaggttttc tgtgatattt
     6001 tcaccaattg aaccatctcc acgtgttacc ccagcaacca aaatcccctt ttcataagtc
     6061 agcgagatag ataagccatc gattttcagc tcacaaatat aggtcggatg agcaacttcc
     6121 ttacgaacac gcgcatcaaa agcatctagc tcctcacgtg aaaaagcatc ctgcaaacta
     6181 taaagaggat actgatgact gtatttttca aaaccatcta aaaccttgcc accaacacga
     6241 tgagtcggac tatctgctag cacttgctct ggataagcag tttctaactc gaccaactca
     6301 cggtaaaggc ggtcatactc actgtctgaa accgagggat tatcgctggt atagtactca
     6361 gtcgcatagc gattgagcaa agcgactaac tcattcattc ttttattcat aagaccattt
     6421 taccataaaa caaaccctcc tcacaaacga gaagggcgga aaaaacactt agtttgaaat
     6481 tatttttgaa actcaagcaa ccttatatca atttttcaaa atgagttcga acataaataa
     6541 acgatataca agacaagatg ataacaccac ttccaattat caggaaagaa gagagatgta
     6601 cacttggcaa gactgtcata aatccttttg caatgggcat aaatagaata gctaaggtaa
     6661 aaattgtact cagtactctt ccaagaaatt cgctctcaac cttggtttgt acttgagtaa
     6721 aaaagtgaat attaaaaatc gtcataaaca attcacaaac taaatttcca gaaaaggaaa
     6781 gaaaagttgg aagtggtaat cccatcataa aaactccgac acctgtcaaa gccagtaaaa
     6841 tcaaaagatt ataaatatta gctttaattt tactagctag aagagcccca atgatggaac
     6901 caatagcccc catagttaaa atacttgcat aggctccttc tgacccgtaa agctgattcg
     6961 aaaagggaag tagaaattca aaagctgcaa aaaagaaatt aacgctggaa gctaccagca
     7021 aaaagaagaa aatttcttgc tgatgccaga tatagtgtaa cccatccttg atatctacaa
     7081 aaatatctct cccagtaaaa gcctttttct cttgaacttt tgcttcctct tttggaagga
     7141 aagccactag aacaaaagca atgaaaaaag tcagcgagtc tagcagtagc gtcatatgga
     7201 gacttgcaaa ctgtaaaaca aggaaggaaa gaacaggaga gctaacacct acaacctgca
     7261 aaaccagctc taagcgagaa ttatagatca caatctcatc tttctccacc acttcagtta
     7321 tgatagcttt attggctgtg cgagaaaagg caaaagcaat agcctgcaca atgttagcaa
     7381 caatcaaagc gccaatcatc cagctatcat tccttatgaa agaaatagcc agacaaagaa
     7441 tcccacaaac aagatctgcc gtcattaaaa tcttacgacg agaaaaacgg tctgaaataa
     7501 ctccgccaaa gggattgacg ataatagatg tgacgagctc agaaatctga tacattccta
     7561 aaactgtctg tcctatagtc cccatagaag ccaaccagac actatttcca taatcataga
     7621 gcatatttcc cattttattg atagccccac ggctaatcaa ctgcactgca tagcgattca
     7681 tattaaagct cctctcaaat tttgaaacta ttgtatcaaa accgaaagga gctttttatt
     7741 ttttccctta tttgggaaaa ttaacttttg acaaattttt cgtagtgttc ctgataatag
     7801 gctacttgct ctggaagacc taacacatca aaaatatgca tggtctcttg catctgctta
     7861 cagccttctt tacactgtcc tttttgatat aaggcaaaac cttttaaata atggaaaaca
     7921 ttacgctcat aaagcttaat acctttgtca ataatcttct ctgtataagc ctcaaaatag
     7981 ttggcattat aaaaagaaga atgctctaaa caatgctggt aacaattgag ggccaaaatc
     8041 aacactaatc tcttatggcg actaatctct tggtaaaatt cctccctctc cataacttct
     8101 ctaccaatcc gagtgacata gtctacatcg tagaaactat agaggttacc gaaaagaatc
     8161 aactcataca tggtccattc ttctgttttg aagagataat ctgctacctt atccaaatca
     8221 tcctgcttca tatcataact cgcatctctt tgacaaatca gaccttgtag caaaatccag
     8281 ttcagctcaa aataaagggg agtcgtcgaa ctcttagact tttcaagttg ttctctttga
     8341 agcttttgaa aacctgcaat atcgtttgaa tagtaaagtg ggataatctg tgccatcata
     8401 gacacatgtt catgattatg aaaattcctt gccttatcca tgaaattttc gattgttaca
     8461 tgaatgttat ccaaaatctc aaagaaacgg gagactgcca ggtcagactc cccaagctca
     8521 aagcgagata actgagaggt agagcaggat tcgcctgctg cttcctttaa agaataattt
     8581 ccacttgttc gaaattcacg aaatactttt ccaagatgtt ccatctttac acctgatctg
     8641 ataattcttc ccactcaagc atagcttctt cctgacgatg gctgattttg tccagctcag
     8701 cctgtaattc catgagtttg tcggcatcgt ttgtttccaa catttgttca gaaatggctt
     8761 ggctttgact ttctagctct tcaattttag cttctagact ttcgatttgt cgcatgagtt
     8821 tgcgaacttc tttttgactt tctttctggg cctgatagtc attgactgga cttgcttcct
     8881 ttgcttgatt gctagttgaa gcttcctcag tctgactcat ttctgctgtt gctttcttct
     8941 caacatagta gtcgtaatct ccaaggtaga gagttgaacc attctcagac aattccaaaa
     9001 catgagttgc cacacgattg ataaagtaac gatcatgact gacaaaaagc aaggtaccat
     9061 caaagtcaat caaagcattt tccaacactt ccttgctatc aatatccaag tggttggtcg
     9121 gctcgtcaag gatcaggaag ttgttgtttt ccatagacaa tttagctaaa agcaaacgag
     9181 ctttttcgcc accagatagc atgccgactg attttttaac atcatctcct gagaaaagga
     9241 aggctccaag acggttgcgg atttcaactt ctggtgtcag tttgaaatca ttccagagtt
     9301 catccagcac cgtattactt ggtgtcagct tgctttgggt ttggtcatag taaccaacct
     9361 caacattagc gccaaagcgc ttttcacctt tgataaaagg aatctggtcc acaatagact
     9421 tgataaaggt tgacttgccg ataccatttg gaccaacgat agcgacagca ttcatcttac
     9481 gaagatctag gttgataggt tgtgacaaga cttccccgtc ataaccaata gcagtatttt
     9541 caacagtcaa aacaacattg cccgacgttt tttcagactg gaaggtcatg ttggctgctt
     9601 tcttgccagc ttcaggcttg tccaaacgct ccattttttc tagttgttta cggcgagatt
     9661 gagcacgttt agtcgttgaa gcacgaacta gattgcgatt gacaaagtct tccagagcag
     9721 cgatttcctt ctgttgcttt tcatagtttt ttgcctcagt agctagcttt tgctccttca
     9781 attcgacaaa acgagagtaa ttccccacat agcgatccaa ggaatgcttg gtcaaatcta
     9841 gcgtaattgt cgcaaccttg tccaagaaat aacggtcgtg gctgacgata atgagggcac
     9901 cgctatagtt taccaagtaa ttctctagcc aggcgatggt ttcaatatcc aagtggttag
     9961 ttggctcgtc caagaccaag agattgggct tttcaaggag cattttggca agtgccaaac
    10021 gagtattttg accaccagaa agctcagcaa ttttcatctg ccacatagac tcgtcaaact
    10081 tgaatccatt caaaatcgct cgaatatcag cttcataggt aaagccacct gcttggcgaa
    10141 aattctcaga taagcggtca taatctgaca tcagtttatc caaatcctca ccagattttt
    10201 cacccatctc cagctccatc tgacgcagtt gtctctccgt ccgacgcaaa tcattaaaga
    10261 catgaagcat ttcatcgtag atggtatttt cagactcaaa acggctatct tgggctaggt
    10321 aagacagaga aatatctttt ttcttattga tttctccgct agttggctcc tcttctccaa
    10381 ctaaaatctt caaaagagta gacttacctg caccattttt cccaacaaga gcaatccgat
    10441 ctcgttcatc aacctgcagg ttgatattat cgaaaagaac ctctcctgca aaagaacgtt
    10501 caattttatt agcttgtaaa ataatcatac aagtagtata gcatgtttcc ctaagtcatt
    10561 caagataatc gtaagtcttt tagtacaact tttataacat a
//
LOCUS       DAVKAO010000059        10295 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKAO010000059 DAVKAO010000000
VERSION     DAVKAO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.059"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            51..401
                     /locus_tag="VWX16_001594"
     CDS             51..401
                     /locus_tag="VWX16_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9158916.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            616..732
                     /locus_tag="VWX16_001595"
     CDS             616..732
                     /locus_tag="VWX16_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9158917.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            725..976
                     /locus_tag="VWX16_001596"
     CDS             725..976
                     /locus_tag="VWX16_001596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158918.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGLSVSEFEPFCVEKSPI"
     gene            1020..1298
                     /locus_tag="VWX16_001597"
                     /pseudo
     CDS             1020..1298
                     /locus_tag="VWX16_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1435..2403)
                     /locus_tag="VWX16_001598"
     CDS             complement(1435..2403)
                     /locus_tag="VWX16_001598"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9158919.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2548..3090)
                     /locus_tag="VWX16_001599"
     CDS             complement(2548..3090)
                     /locus_tag="VWX16_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9158920.1"
                     /translation="MDQLLGVLFPDSTFLNAFESAIVAPLVEEPLKLLPLVFVLALIP
                     VRKLKSLFLLGIASGLGFQMIEDIGYIRTDLPEGFDFTISRILERIISGIASHWTFSG
                     LAVVGVYLLYRAYKGQKVGKKQGLIFLGLALGTHFLFNSPFVELETELPLAIPVVTAI
                     ALYGFYHAYCFVEKHNELMT"
     gene            complement(3094..3378)
                     /locus_tag="VWX16_001600"
     CDS             complement(3094..3378)
                     /locus_tag="VWX16_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158921.1"
                     /translation="MKKSSILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFLALFYM
                     IPALYFLFHIGKKWELPKKVLILSLLGAICSFTSLLLFGIYNHRRKSSKV"
     gene            complement(3403..3900)
                     /locus_tag="VWX16_001601"
     CDS             complement(3403..3900)
                     /locus_tag="VWX16_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9158922.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(3973..5334)
                     /gene="radA"
                     /locus_tag="VWX16_001602"
     CDS             complement(3973..5334)
                     /gene="radA"
                     /locus_tag="VWX16_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9158923.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(5348..5863)
                     /locus_tag="VWX16_001603"
     CDS             complement(5348..5863)
                     /locus_tag="VWX16_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9158924.1"
                     /translation="MKIIFVRHGEPDYRELEERFYIGFGIDLAPLSEMGRQQVQKLSK
                     TPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(5865..6308)
                     /locus_tag="VWX16_001604"
     CDS             complement(5865..6308)
                     /locus_tag="VWX16_001604"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9158925.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(6368..6461)
                     /gene="ffs"
                     /locus_tag="VWX16_001605"
     ncRNA           complement(6368..6461)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VWX16_001605"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(6494..6961)
                     /locus_tag="VWX16_001606"
     CDS             complement(6494..6961)
                     /locus_tag="VWX16_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9158926.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(7162..8448)
                     /locus_tag="VWX16_001607"
     CDS             complement(7162..8448)
                     /locus_tag="VWX16_001607"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9158927.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(8679..8915)
                     /locus_tag="VWX16_001608"
     CDS             complement(8679..8915)
                     /locus_tag="VWX16_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9158928.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            9181..9978
                     /locus_tag="VWX16_001609"
                     /pseudo
     CDS             9181..9978
                     /locus_tag="VWX16_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10013..10222)
                     /locus_tag="VWX16_001610"
                     /pseudo
     CDS             complement(10013..10222)
                     /locus_tag="VWX16_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3307 a         2408 c         1710 g         2870 t
ORIGIN      
        1 agagttattc acatcttatt tcaactcact atagaaggag gaataggagg attctcagac
       61 atccgggcat cagcccaact aatgatttga ttgctaagaa aatattcagc aatccagaaa
      121 tcacttgtca atttattcgc gatatgctgg acttgccagc aaaaaatgtg accattttgg
      181 agggaagcga tattcacgta ttactctcca tgccttactc agtgcaggat ttttatacca
      241 gtatagacgt cttggcggag ttggataacg gtactcaagt aattattgag attcaagtcc
      301 atcatcagaa ttttttcatc aatcacttgt ggacttacct gtgcagtcag gttaatcaaa
      361 atcttgaaaa aattcgtcag cgagaaggtg atactcacta gagctacaaa cacatcgctc
      421 ctgtttacgc cattgctatc gtggatagta attatttctc agatgacctg gcttttcata
      481 gctttagtat gcgcgaagac acaacaggtg aggtattggc gattaccaac aatggacagg
      541 aaaaccatct ggttaagatg gcattcttgg aattaaaaaa tacagagaaa ccagcaagga
      601 cgaggttcgc aagccatggt tggagttttt cggcaacaag ccctttaccc agcaaccgca
      661 acgagccatt acccaagcaa atcaactgct ggactacaag agctggtccg aggaggacag
      721 gaaaatgttt agtcaactac atatgcgaga agaacaagtc ttgttagcac aggactatgc
      781 cttggaaact gctagggctg aaggccttga acaaggacta gagcgtggga aagttgaagg
      841 aagggcagaa aggaaacttt ttgccttcct agacatagta cgccaaggtc ttctgacttc
      901 tgaggttgcc agccagcaat taggtctgtc agtatctgaa tttgagccat tttgtgtaga
      961 aaaaagtccc atataaccta taatgaaaag cgacaaaaca actcattaga aagaatcata
     1021 tggaacaatt acattttatc acaaaattac tagacattaa agaccctaat gtccagattt
     1081 taaacatcat caataaggat acacacaagg aaatcatcgc caaactggac tacgacgccc
     1141 catcttgccc tgagtgcgga aaccaattga agaaatatga ctttcaaaaa ccttctaaaa
     1201 ttccttatct tgaaacgact ggtataccta ctagaattct ccttagaaag cgtcgattca
     1261 agtgctatca ctgttcaaaa atgatggtcg ctgaaactta tgatgacgta cagtcatatt
     1321 tcttctcttt ttattatatc acagttttaa atctagcttt actagattca ccgctactat
     1381 ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat ctcaggcttg gtcattaaat
     1441 ttttttctca atatcgaaaa gtggagaaag tggtcgtttt tcatgaatac gtacgatagc
     1501 atcccctagg agatgagcga ttgaaatctg ctcaatctta tcaatcaaac gctcttctgg
     1561 cagatagatg gtatccaaaa caaccaattt cttaatagct gatttttgga tattgtccgt
     1621 agcaggacca gaaagaactg ggtgcgtaca gcttgcatag acttcaacag caccagcttc
     1681 cgcaagagca tctgccgcat gacaaatcgt tccagcggta tcaatcatat catcaatcaa
     1741 gatacaagtc ttgccttcaa ccttaccgat gatgttcata acttcactag tattcatctt
     1801 atcaacgcta cgacgtttat caataatagc gatagatgtt ttcaaaaatt ctgccaactt
     1861 acgagcacga gtcacccctc catggtccgg gctgacaacc acatagtcag aaccaaccat
     1921 accacgacgc tcaaaataat ctgcaatcag aggagcaccc atcaaatgat ccacaggaat
     1981 atcaaagaat ccttgaattt gcgcagcatg caagtcgatg gtcaataaac gatccactcc
     2041 agctacttca agcatatttg cgacaagttt tgaagtgatt ggctcacgcg ctctcgcctt
     2101 tctatcctga cgtgcatacc catagtaagg catgacaaca ttgacagatt ctgcactcgc
     2161 acgcttcaaa gcatctacca taatcaaaat ttcaagcaga ttgtcattta caggcgaact
     2221 agttgattgt aagataaaga cgtgtttccc acggattgat tcttcaatgt tgacctgaat
     2281 ctctccatct gaaaattggc gaacacttga tttccccaac tctatcccaa tctcctgcgc
     2341 cacacgttct gccaattctt tattagaaga aagggcaaac agctttaaat cagaaaaaga
     2401 catgatttcc tccggtatat atgtataact tgtgtttttc acaagatttt ccatctacca
     2461 ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa gaagggcacc atatttgtac
     2521 ccttgcatca ttcttttgaa aaatattcta ggtcatcaac tcattgtgtt tctcaacaaa
     2581 gcaataagca tgataaaaac catagagagc aatagccgta accactggaa tcgctaaagg
     2641 caactctgtt tccaactcca caaaaggaga gttaaacaag aagtgagttc ccaaggctaa
     2701 acctagaaaa ataaggccct gtttcttgcc aaccttctgt cctttatagg ctctgtaaag
     2761 caagtaaaca cctactacag ctagacctga aaaagtccag tgagaggcaa ttcctgagat
     2821 gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc tctggcaaat ccgtacgaat
     2881 ataaccaata tcctcaatca tttggaatcc caaaccggaa gcaattccaa gtaaaaacaa
     2941 agattttaat tttcgcacag gaatcaaagc caaaacaaaa acaagtggca ataatttcaa
     3001 gggttcttct accaaaggag ccacaatagc actttcaaag gcatttaaaa atgtactatc
     3061 tgggaaaaga acccccagta actggtcgat tttttatacc ttagatgact ttcgtctgtg
     3121 attatagatt ccaaatagta agagagaagt aaaggaacag attgctccca ataaagacag
     3181 aatcaaaacc ttctttggca attcccattt tttcccaata tggaagagaa aataaagagc
     3241 cggaatcatg taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc
     3301 tgacctcgaa ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa
     3361 aatactgctt ttcttcatac actttctttc taaatgaagt atttataatt ctacgactgt
     3421 catacttcct gtatcaacat tgtaaatagc accagagata atgacatcgt ctggtattag
     3481 gggagactcg ataagcagtt gcatatcctc gcgtacactc tcttctatat cttggaaggg
     3541 caagaagtcc tggtctgaca catctacacc taattcctct tttaaatact cctgaaaagg
     3601 ttcattttca aaggtctgag caccacagtc tgtatggtgc aataccacaa tctctcttgt
     3661 ccccatttgt tgctgggaaa taactagcga acgaatcatg tcttcagtca ctcgaccacc
     3721 tgcattccgc aagatatgag catccccaag tgccaagccc agagcttgcg caacgtgcag
     3781 acgagagtcc atacaggtca caatagctac tcttgttttg ggtttaagtg gcagatttaa
     3841 ctgcccatgt agggcaacat aagcctgatt agcttgcata aactgttcaa aatacgacac
     3901 gattccctcc ttgaaaattt gatagtcaaa tatttctcct atcttatcat ttttaagaga
     3961 atttgtcacg gattatgcaa agaccttttt caaaacttcc tgaatcgttg tcacgccaat
     4021 gacctgaatt tccttaggca gagtgattcc tgtcaaggaa ttcttaggta cataaatctt
     4081 agtaaagccc agtttagcag cttcgttgat gcgttgctca atacgattca cgcgccgaat
     4141 ctctcctgtc aagcccagtt ctccgacaaa acattcctga ggattagttg gcttgtcttt
     4201 gtagctcgaa gcaatagcaa ctgcaacagc caagtcaatg gcaggttcat ccaatttaac
     4261 accaccagca gatttgagat aggcatcctg attttgtaag agaagccctg cccgtttttc
     4321 caaaacagcc ataatcaagc tagcacggtt aaaatcaagt cctgtcgtag tacgcttggc
     4381 atttccaaac atggtcggtg ttaccaaagc ctgaacctcc gccaaaatcg gacgcgtccc
     4441 ttccatggtt acaacgatgg aggaaccagt cgccccatcc aaacgctctt ctaggaaaac
     4501 ttgactcgga ttgagtacct caaccaagcc gcccgactgc atctcaaaaa tcccaatctc
     4561 attagtggaa ccaaaacgat ttttgaccgc tctcaaaata cgaaaggtgt ggtgacgctc
     4621 cccttcaaag taaagcaccg tatccaccat atgctccaac atacgaggcc cagccaaggt
     4681 tccttctttg gtcacatgac ctacgataaa gatggcaatg ttattggtct tggccaactg
     4741 catgagttca gcggtcactt cacgcacctg agaaacagac ccctgcaccc ctgaaatctc
     4801 aggagacatg atggtctgga tggaatcaat aatgagaaag tctggctgga tacgctccac
     4861 ttctgcacga acactctgca tattggtctc tgcatagaga taaaactcac tatcaatatc
     4921 acctaagcgc tctgcacgta gtttaatctg ctgggcagac tcctccccac tgacatagag
     4981 aactgtcccc acttgggaca actgggttga gacttgtagg agaagagttg atttcccaat
     5041 cccaggatcc ccaccgatga ggacgagact tcctggtaca actccgcctc caagcacacg
     5101 gttgaattcc tccatctccg tcttggttcg attgacattg atggaagtca cctcagctag
     5161 tttcatgggc ttggttttct cacctgtcaa ggacacacgc gcattcttga cctcggcaac
     5221 ctcaacctct tccacaaaag aagaccaaga cccacagttg gggcaacgtc ccagatattt
     5281 aggggaatta tacccacaat tttgacatac aaatgtcgct tttttctttg cgatgacaaa
     5341 cctctttcta tatctctaac tcacactcaa tcacttggca aaaatcaatc ttctcatttg
     5401 gcacaaactg gcgcatgagc attcgatgag caacaactac cacagtctga tgttctcgat
     5461 acttagacat acattctaga aaccgagact tcatttccgt agctgtctca tattgaatag
     5521 gactattagg aagcaactcc cccttgtttt ctaaaaacag tcttctagct gtttcaaagt
     5581 tttctattcc tgttttatag acctgccatt catgtaataa aggctctact cttaaaggaa
     5641 gacccgtagc acagaccaca tacgaagccg tttctaaagc tcttgtgact gcagaagata
     5701 cgattatttc agctgacgag agtaaaggag ttttgctcaa tttctggact tgctgccgtc
     5761 ccatctcaga caagggtgcc aaatctatcc caaatcctat ataaaaacgc tcctctaact
     5821 cacggtaatc tggctcccca tgacgtacaa agataatctt cattctagtg ccctgtcgat
     5881 ccaaatccac cagttcgaac gccatcagcc gcatctccat ctgcaattaa gaaagtagca
     5941 aaaacagcct ggacaatacg ctccccaact tcaagaacaa cctcttggtc tgtgatattc
     6001 ttcatctgcg caaaaatatg cccttcattt ccaggatttc cataataatc cccatcaatg
     6061 accccaactg agttaattaa aaccaagccc ttcttacgag gatttgaaga acgatcatag
     6121 aggtagagaa cctcagtcgg ctgcatataa gccttaaccc ctgtcggaac caagacaatc
     6181 tctcctggcg caacaactgt acgcacagca acctttaagt cgtaaccagc cgcatgcgct
     6241 gtctcacgct tgggcaataa attttcatct gtaaaactcg aaaccaattc aaaaccacga
     6301 attttcataa ttttctcttt tctattatca tttattctag attattctat cttatttatt
     6361 cggaaaaagc acgaaaaaag agcacacaat tcaaatcgct tagggctgct ggattcctcc
     6421 cctgacccgc ttcacgcaga actgttgctc cactatttat tatatcacat tcctattcat
     6481 tttaaaagca aaattatttt ttccgtctat ttctaaaaaa atcctgcatg atagctgcgc
     6541 attcatcttc caaaattcct atttcaacct ccacacgatg attgagacgc tcatctgtca
     6601 agatatcgta caaactccca gcagcgccaa atttctggtt tttagcccca tagaccacat
     6661 ttggaatgcg ggcaagccca atcgccccac tacacatgac acaaggttca atggtcacaa
     6721 aaagtgtgca atccagcaag cgccagctct cctcactcaa gttcgcatcc tctatagcca
     6781 taatttccgc atgcataacc gctcgctgta attcctcacg cgcattatgc ccacgaccaa
     6841 tgatttcccc atctttgaca atcacacaac caattggaat ttcatcgtgt tcaagagcaa
     6901 tctcagcctc tctcaaagcc tccctcataa agacttcttt ttcttcaagc gtataataca
     6961 tctcttttct cttttcctac ttatcgattt tattattata tcatgaatcc caacacaaaa
     7021 aaagccaccg aatgcggtga ctctataggg agattattat gaaaaagaaa agtttaggat
     7081 atttgttaca acaagttagg aggtcttctt gtaactgtct atagtatacc cgacctatct
     7141 taaacaaatc ttaaaaatct cttaggacca aacactttct aaaatatttg tttgttcacg
     7201 accaggacct actgagaaag tagaaatacg aacgccaacc aattcactca cacgacgaac
     7261 atagttacgc gcattctcag gaagatcttc caaattgcga actccggtaa tatcttctga
     7321 ccaacctggc aactcttcat agataggctt gcaacgtttc aattgctcaa gactagctgg
     7381 atagtagtca atacgttgac cgtcaagatc ataggccaca cagattttca cagtatccaa
     7441 accgctcaaa acatcaatag agttcaaaga aaggttagta ataccagaaa cacgacggct
     7501 atgacgcatc acaactgagt caaaccaacc tacacgacgt ggacgaccag ttgttgtacc
     7561 atactcatga cccacttcac ggatacgttc tcccacttca tcaaacaact cagttgggaa
     7621 aggaccatct cctacacgac tcgtataagc tttacataca cctacaacct tgtcaatctt
     7681 gcttggaccg acaccagaac caattgtcac accaccagct acagggtttg atgacgtaac
     7741 aaatggataa gtaccttggt cgatatctag cataacacct tgtgcacctt caaaaagcac
     7801 acgtttgcca ttatcaagcg catcattcaa gataacagat gtatctatca cgtatttctt
     7861 gatttgttga ccatattcgt aatattcttc aaaaatatca tcgaaaacaa tcgctttact
     7921 gtcatacaat ttttcaaaaa gacgattctt ttcagcaagg ttacgttcta aacgctcacg
     7981 gaaaatatct ttatctaaaa gatctgcaat acgaattcca acacgagcag ccttgtccat
     8041 ataagctgga ccaattccct taattgtcgt accaatctta ttgtcgccct tagcttcttc
     8101 ttgcaagcga tccaactcga tatgataagg caaaataaca tgcgcacgat cagaaatacg
     8161 caagttatca gttgttacac cttcctcatg aagatagctc aactctttta caagagattt
     8221 aggatttaca accataccat tcccaatgac agatattttt tcagggaaga aaatcccaga
     8281 tggaatcaag tgcaacttaa atttcttacc gtcaatcaca atcgtgtgac cagcattatc
     8341 accaccttgg taacgtgcaa tcacttctgc attcgctgaa aggaagtctg taatcttccc
     8401 tttaccttca tcaccccatt gggtacctac aacaacaact gaagtcataa tcttgtctga
     8461 gccctcaggc tcttcctttc tcacatacat ggcaggactc tcacctgcaa ttatatctta
     8521 caatttatta taataaaaaa tcgccttttt atcaagaaga aacaatagaa agatttgcta
     8581 tttccaacta ttaaaaaatg atttagaaaa attactagct atttactatc atctttccat
     8641 aaaagagtaa attagttcgg aaatttacta aaattacctc aacaagaaat aaaaccccga
     8701 ttcattacca atttttcaag atacaaacga taagcaacac gataatggta aacgataaaa
     8761 tccctacgac aaccaatgcc atatctcact aaataataaa ttaaaaattt aaaatgaaca
     8821 tgttcccagt caaaattatc accaaatgta ggaccatact cttcttcaat actatcatag
     8881 aaattagcca tctgctcata aattttttgt aacataatca aatactcctt ttctttttta
     8941 taaacttatt ctaacaaaaa attttacaca ttcactatca attcctgaat tgttaaaata
     9001 ccagcctcaa caagataaaa ataggaaaag ttgacaaaat agaaacaaat ttgttaccta
     9061 aatttcaaac aattaaagtt ctaaaaagac cgccttaaaa cttttgggta aatcctatta
     9121 gaactataat atttttatat aggtattatg gttctgattt tctagtataa aaaaattcaa
     9181 actagaattc ttcttagaaa aggccgtttt aagtgctatc attgctcaaa aatggagctc
     9241 gctgagactt ctatcgtcaa gaagaatcat caaattcctt gtatcatcaa ccaaaagatt
     9301 gctcagaagc taattaaaaa gacttctatg accgacattg atcatcagtt gtctatttca
     9361 acttcaactg tcattcgcaa gatcaatgat tttcactttg agcatgattt ttcgcgtctt
     9421 cctgagatta tgtcctagga cgttgaaaca gtccggggag tgactgtttc aatcgggaga
     9481 tggagatgag ctttattgct caagactttg ataagctcaa tatcataact gttcttgaga
     9541 gcagaacaca agccatcatc cgaaatccca tgaatacaag gctatcaagc gatactgaaa
     9601 gctcattcaa caagatagtc agaaactgag tgataaacga ttttatcgcc ctacttttcg
     9661 tatgcacttg actaataaag aaattctaga taaacttttg agctattccg aagacttgaa
     9721 acaccactat aatctctacc agctcttgct tttccacctc tttttcagac tctgtttaaa
     9781 accttcctca aagataaaga gaaaatcgtc aacgcccttc aattacctta ttccaacgcc
     9841 aaactggaag ccaccaataa tctcatcaaa cttatcaagt acaatgcctt tggttttagg
     9901 aactttgaaa acttcaaaaa agaaaggacg aaatttgtcc tttctagatc ttcgctttct
     9961 tcaacccact acagttgaca aagaaccgaa aaatgtctcg cctctaatat agtcaattga
    10021 aacaagaaca agacaaaaga gcctcataaa aggtattgca acttggtaat acctttttgg
    10081 ggttcttttt gatatgaacc catgttttct caataggatt gtactcaggt gagtagggag
    10141 gaagaggtaa aagtttatgc ccaaactctt cacacaagag ttctagctta cccattctat
    10201 ggaatcttgc attgtccata atatagtaga ttgaaactag aatagtacac ctctacttct
    10261 aaaacattgt tagaattcga ttttactgtc ctgat
//
LOCUS       DAVKAO010000060        10177 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKAO010000060 DAVKAO010000000
VERSION     DAVKAO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.060"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(98..1312)
                     /gene="thiI"
                     /locus_tag="VWX16_001611"
     CDS             complement(98..1312)
                     /gene="thiI"
                     /locus_tag="VWX16_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9158929.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGFVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(1321..2463)
                     /locus_tag="VWX16_001612"
     CDS             complement(1321..2463)
                     /locus_tag="VWX16_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9158930.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            2601..3029
                     /locus_tag="VWX16_001613"
     CDS             2601..3029
                     /locus_tag="VWX16_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158931.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKKDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            complement(3180..5405)
                     /locus_tag="VWX16_001614"
     CDS             complement(3180..5405)
                     /locus_tag="VWX16_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9158932.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     INENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(5569..7521)
                     /locus_tag="VWX16_001615"
     CDS             complement(5569..7521)
                     /locus_tag="VWX16_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9158933.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIEEMIKSLTGHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALTELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLAISGDAEVYRAANGAK
                     VATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(7518..8429)
                     /gene="pfkB"
                     /locus_tag="VWX16_001616"
     CDS             complement(7518..8429)
                     /gene="pfkB"
                     /locus_tag="VWX16_001616"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9158934.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNISNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVKLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(8426..9166)
                     /locus_tag="VWX16_001617"
     CDS             complement(8426..9166)
                     /locus_tag="VWX16_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9158935.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            9328..9415
                     /locus_tag="VWX16_001618"
     tRNA            9328..9415
                     /locus_tag="VWX16_001618"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9362..9364,aa:Ser,seq:gga)
     gene            complement(9585..9932)
                     /gene="ssrA"
                     /locus_tag="VWX16_001619"
     tmRNA           complement(9585..9932)
                     /gene="ssrA"
                     /locus_tag="VWX16_001619"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3014 a         2311 c         1943 g         2909 t
ORIGIN      
        1 tatcatgaaa atagaaaaaa gttagttttt tacctgtttt tagaaaaaaa aactaacttt
       61 ttttactgat tttcgctatt cttttggatt ttctgaatta gagtagatta tcaatcaagt
      121 catcaacttc atctttttcg gcttgaggtg tgatttcagt aatcatgatt ccagccactg
      181 ctcgctcaac caagccttca acatccatac gcgcttcgta ctgctccgca ttcttaattt
      241 taggatttgt ttttggacga tctggtgcaa aaatggtaca acagtcttca aacggttgga
      301 ttgaaatgtc aaaggtatcg atttcctggg cgatgtcaat gatttccaac ttgtccatgg
      361 taaccacagg acgaatgatg ggagtgttgg taacagcatt gatagccttc atactttcaa
      421 gggtttggct ggctacttga cctagacttt ccccattgat gataacaaaa ccatttcgta
      481 cctcacgaat acggtcagta atccgcatca taaagcgacg agttagagtc atcaaataag
      541 cttctggcgc tttggctttg atttcctctt gaatctctgt gaaaggcacc tcgataaact
      601 ggatatttcc gccaaacttg gtcaatttac gggtcaagtc ctgcgctttc ttgagggcac
      661 caggactagt atatggtgga ctagcaaagt gaactgcctc gatatccacc ccacgcttaa
      721 gagcaagata acctgctaca ggtgagtcaa tccctcctga caacatgagc atccctttac
      781 ctgaagttcc aactggcaaa ccaccagccc cacgaatggt ttcataagaa agataggctg
      841 cttcttcacg aatctccacc tgaagattga tgtcaggact tttcatttga acttgcacat
      901 ttggaatggc ttcgaataca gcccctccaa gtgtttggtt gagttcacga ctatcaagtt
      961 caaagttgtg gtcgctacgc ttgctagaaa tcttaaaggt cataccttcc ttgtagatgt
     1021 cccgcataat ctcttggaca gaagacttca aaacttctac agatttttca accttataaa
     1081 caggagaaaa gttttgaatt ccaaaaactt gtttgagaga ttctgcaact gctgtgtaat
     1141 cagctccatt gaggtaagcg tgggcacggt cgcgatctgc tgttacctta acttgggtat
     1201 agatagacaa aacgtccgaa atattattac gaagtttatt gatgaaacgc atacggtttt
     1261 tacccttggt tgacaactct ccgtagcgaa tcataatttc tgaatactgc atgaatgctc
     1321 ctatcttact tttctagttt gattgtaaat caattttaac ttggtcaaaa actgctcgac
     1381 ctgactcata tcattttcca agtctaggct aagacgcaca gctgacttgg ccttatcttt
     1441 gtccactccc atggcaatca aggtaccggc tggttttcct gccttagatg aacaagctga
     1501 ggttgttgag atgaaaatat catagtcttc aaaggcgtga acgatgactt cacctcgaac
     1561 acctttgatt ccaaaagtca gaatatgagg tgcaaagtct tcctcatctg aaaagacaaa
     1621 aatatccgga tagttcagaa gagcttggtg aatcactgcc ttcatctgcc cagtcttgct
     1681 cctaaagata tctagctttt ccatagataa acggagagcc ttggctgtcg ctgcaatccc
     1741 tgccacattt tcagttgtcg aacgataatc tcgctcctgg ccaccacctg taagaagagg
     1801 tgtaatcttc ttgccagatt tgatatagac aaagccaaca ccacggacac catgaaattt
     1861 atgactcgag aaggtcgcgc aatccacccg ttctgtcaga tacttttcag tcggaatttt
     1921 ggcaagcgcc tgaaccgcat caacgtggaa ggaaatagtc ggcttgtctg ccaagaattc
     1981 tgaaatagcc tcaataggtt ggatagagcc gatttcattg ttcacagcca tgatggaaac
     2041 gaggattgta tcaggccgta tcaaacctgc taacgcctca acatcgacca agcctttctt
     2101 atcaactgga gcaaaatcca cttcaaatcc ttgacttttc aaccagaggg ctgactcttt
     2161 gactgctgga tgttcaatgg ctgaaacaat gatgtgcttg ccaaactgag ctttttcaaa
     2221 ggccacaccc ttgataaccc agttatcccc ttctgttcca ccagaggtaa agaagatttc
     2281 atcgcttttc ttaccgatta aatctgcaat ctgttggcga gaagcatcta agattcgtgt
     2341 tgcttggtct cccaaacgat ggagactaga tgggtttcct aaaatttttg aagcgacctg
     2401 catataggtt tcaagggctt caggataagg cttggtcgtc gctgaattat caaagtaaat
     2461 catgtttctc acgctttcta aaatcactcc ctctattgta tcatgaaacg gagcttgcga
     2521 caagaaaggg caatttctct ttttttaaga tttttttaaa gaaacagagt ataataaatc
     2581 ataatcaaag gagagaatgt atgtctaact atcgtagaac ttcaaaaccg aaaacagaac
     2641 atatcaaaaa aggctttact gtttttcaaa aaaccattac tactatcggt agtatccttg
     2701 gtttgattac agcgggtatc acgatcatga acgccttgga taataataat aaaaatacta
     2761 aaaaagaacc tacgacaagc cagacgacaa cctttgtcaa agaaattcaa aaagaatccc
     2821 ctcaggaaaa tactactcca aataaggaaa ataacacttc tcaagaaaaa acacaacaag
     2881 aagaaacgcc aaaatctagc gtcaaggaag agaaaaaaaa agatcagaaa acagcaactc
     2941 aggactctac tacacctgct acaagtaaac ctgccactga aaatgaaaaa cagcccaata
     3001 ctccaacttc agaaaataat actcaatgaa aatcaaagag caaactagga agctagccgt
     3061 aggcagtact tgagtacggc aaggcaaagc tgacgtggtt tgaagagatt tgcgaagagt
     3121 ataaaagtaa tcaatagcca gtaaaatagc tccttccaac cttggaaaga agctattttt
     3181 tattgctgca atacttttct tggcttggta ccttcagctg gaccaatgac acctgccatc
     3241 tcaagctctt ccatgagacg ggtcgcacgg ttaaatccaa ctgacaaacg acgctgaatc
     3301 atggatgcac tggctttctg tgtttcgata accaaagact tagcttcttc aaaaagcgga
     3361 tcaccaccag catctccatc cgaaaattct ccttcatttt cagaaacctc acctggatca
     3421 aaactctcat cgtagtctgc atctgcctga gtcttgatga agttcacaat gcgctcaaca
     3481 tcgtcatccg agataaagga gccttggaga cgaactggat gattttcatt aatcggttta
     3541 aagagcatgt ctcctcgacc aagaagtttt tctgctccat tttcatccaa aatcgtacgg
     3601 gagtctgttc ctgatgaaac cgcaaatgct acacgagatg gaacattggc cttaatcaaa
     3661 ccagagatga catcaacaga tggacgctga gttgcaagaa tcatgtggat acctgcagca
     3721 cgcgccttct gcccaagacg gatgatagca tcttccactt ccttgctggc caccatcatg
     3781 aggtcagcca actcatccac aatcacgaca atgaatggta gcggaatttg cttgtactca
     3841 gactgggaat tgaactcttc taccttggca ttaaaacctg caatattccg aactcccacc
     3901 ttggcaaaga gttcataacg gttttccatt tcatccacaa ccttttgcag agccttgctg
     3961 gctttgcgtg gattggtcac gactggaatc aagaggtggg gaatatcatt gtaaacagat
     4021 aactcaacca tcttgggatc gaccatcata aatttaactt gatctggtct cgccttcatg
     4081 agaatgctag caataatgcc gttaactgct actgacttcc ctgaacccgt tgaacctgca
     4141 actagcaagt ggggcatttt agaaaggtca aaagctcttg cggttccatt aacagccttc
     4201 cctaaaggaa tttccaagaa attttctgct ttcgtttgcg attgttccca tagttctcgg
     4261 aaagatacag tggcaatatc ggagttgggc acttcaattc cgattagaga tttcccaggg
     4321 attggtgctt caattcggac atccttggca gccaaggcta gagcgaggtc atctgataga
     4381 ttggaaatgc ggttgaccct tacaccaaca gccggcttga cttcatactt ggtcactgat
     4441 ggcccaattt cggcccgttc aactgttacc ttaataccaa agctagcaaa ggttgcttct
     4501 aagattttga tattttctct gacaattttc ttctctttag actgatcttt tggtttatct
     4561 ggtgcaaaga gttgtaagct tggaagtttg tattcaaggg cttctttggc tgaaaaatcg
     4621 acctgaacat cttcgtcatc tgagtcatct tcctgttcag ggaatttaag ttcagcttga
     4681 ggcaggatga tttctggttc cacccacttt tcttctggaa taggtggaag attttgaaca
     4741 gcttcctctg tcagaatttc acccgtttcc atatcaacag gaggcaaatc gagtaaggct
     4801 ttttcagtct cttcttgttc taatctagcc tctttctcag ccttttggcg agctttttct
     4861 tcttgtttga caaagcgttc ctcttttcga cgctcgtgcc cttcccacca tttggcaaag
     4921 cctctactga aaaattcagc aatatcgtaa acagaccaag ggctgactag gagagaaccc
     4981 actaaaatca agatagaacc aataaagtaa gttccgatat ttgaaaagag aaaggctgtt
     5041 ggaatataaa gagcgacccc gatcaagccc cctccagcaa agctagtcgt tcgaaaacca
     5101 gtcagatctg tcacaacctg agccatggtc ccttttagaa cggacttgtc caaaccatat
     5161 ttccaaacca agtaggcctc aaaaatcaag agtaagccag caaatatggt gaaaaagcca
     5221 gataagagtc cttcctgttt tcgtatccac ttgaaaaaga agagatagat taataggccg
     5281 aatatcgcca gataagctag gctacccact agcaagcgaa ttaaattata aagggttata
     5341 cctgcagccc ctaatttgaa ggctgcgaaa atcaataaaa tcgcaattcc taacgaaatc
     5401 aacattcgtt gaatcgcttc ttttctttcc agttctgctt tagacggtct ccgtcttgtt
     5461 gtacttgtat tcttgtttgc cattcttcta ttataccata tttcacaggc atttcgggaa
     5521 gagaaaaaga ctgcaccaaa cggtccaatc ttttcatttt tctatttttt atgcttgtgg
     5581 tttgcgtaga taaccataaa ccacaccact tacgattgct cctaccaaga cagaaacgag
     5641 gtaaaggaga gcatttgaag taagggcgat aacgaagatt cctccgtgtg gcgccatgag
     5701 tttgatacca gtaagaccaa cgagtccacc tgctactgct gaaccaagga tgaagcttgg
     5761 aatcgcacga gctgggtcag cggcaccaaa tggaatcgct ccctcagtga taaatgacaa
     5821 gcccatgatg atgtttgtca aaccagagtt acgttcttcc ttagtaaatt tatctttgaa
     5881 aagaagagtt gcgacaaaga ttgcaagtgg tggcaccatt cctccagcca taactgctgc
     5941 catggctaca gaaccacctg aagaaacagt tgctgcaagc gtacctgtac caaagacata
     6001 agctgcttta ttaactggtc cacccatgtc aacagccatc attccaccaa ggacgatacc
     6061 aagaaggaca gctgaacctc ctccaagacc gcctaggaag tcattcatag cagtgttgat
     6121 tgctgccatt gggatattca cagctagcat aacaaatcct gtcaagattg ttccaagaag
     6181 tggcaataga aggattgatt tagcaccttc gagtgaacga ggaactttaa cgtatttctt
     6241 gatggcaaga accaaggcac ctgcgataaa tccaccaaca agggcaccta ggaaaccaga
     6301 tgagacacct gcaagagttg aagttgcttc accacctgcg gcataaggaa ttttaccaaa
     6361 ggcaaaacct tctttggcaa tagcaccagc cacgaaacct gctaccaaac ctggtttttc
     6421 agcaatagag taggcaacat aacccgcaaa gactggaagc atcaaaccaa aggcagctcc
     6481 accaattttc atgaacatag aagctaactc atggtaagaa ccaagattgc caaggttttc
     6541 atttggaaca ctcaaagcac cgtcaatcaa gaaggcaagg gcaatcatga taccaccacc
     6601 gataacgaat ggtaacattt gagatacacc actcatcaag tgtttgtaga aggcaccacc
     6661 aaggctttgt ttttcgttag aggctgttgc gactttggca ccattagcgg cacggtagac
     6721 ttccgcatct cctgaaatag ccaagttgat caattcttct gtcttacgga taccgtcagc
     6781 aactggacga ttgatcaaag gtttgccatc gaaacgatcc atttcaacgg ccttgtctgc
     6841 tgcaatgata atagctttag ccttacggat atcttctgca gttagttgat ttccaacacc
     6901 gctagcaccg ttggtttcga ccttgatacc aacccccatt tcagcagcta ctttttgaag
     6961 ggcttcttgg gccatgtaag tgtgggcaat acctgttgta caagctgtaa cagctacgat
     7021 aaagtcacca gagtcattag caggtgcttg aacaagttcc tcagtttttt ctgaagcttg
     7081 gtcaaaaagt tcgataactt ggtctgcaga tgttgcttga cgaagtttgt ctgcaaaacc
     7141 gtctttcatc aagtattgag acaattctgt caatgctgcc aagtgagtat cattggcacc
     7201 ttctggagct gcaatcatga agaagaggtc agttgcctgc ccatccaaac tctcatagtc
     7261 aacacccttg tttgacttag caaagagaac tgtcgcttct ttgacagcag cgtttttgct
     7321 gtgaggcata gcgattccat cacccaaacc agtagaagtt aaagcttcac gcgccaaaat
     7381 gccttcttta aaggtttcaa aatctgtcac ataaccgtgg cctgttaggc ttttaatcat
     7441 ctcttcaatg acagcagtct tttcagttgc ctgcaaatcc agcaacatga catcttttct
     7501 caataaatct tgaattttca tcgtttttct acctcaactt ttccatatgt ttctttaata
     7561 aattccgccg ttgccaagtc atctgagaag gtagttgccg ttccgcaagc cactccccat
     7621 ttgaaggctt ctactgcgtc ttttgatttg acaaattcac ctgtgaatcc agcaaccata
     7681 gaatcaccag ctccaactga atttttgact gttcctttga ttggtttagc gaagtaagct
     7741 ccctcagatg tgacaagaag ggcaccatca ccagccatag agataataac attttgagca
     7801 cccttagcca gtaactcacg agcgtatttc tcaatttcat ctaaactttc gagtttaacc
     7861 ccaaaaatcg ctccaagttc atgattattt ggttttacaa gaagaggctg gtaatccaaa
     7921 ctatcaatta aggtctgtcc ttcaaagtca cagaccactt gcgcaccagt ctggcgcgtc
     7981 aaggaaatca aatccttata gataacattg cctagatttt tagcacttga acctgcaaag
     8041 acaactgtat cttctgctgt cagactagat aaaatagctt tcaattcttc tagcttaacc
     8101 ggttcaacag ttggacccgt tccgttgatt tctgtttctt ggtctgcttt gattttaaca
     8161 ttgatacgag tatcttctgc cacctggaca aaacgtgtct cgatttcttc ctctgctaaa
     8221 gtatctgtga taaatttacc agtaaagcca ccgataaatc ccgtcgctgt atttgatata
     8281 ttcaaacgtt tcaagacacg gctgacattg attcctttcc caccagcaaa cttatcatca
     8341 ctgtccatac gatttacact accaactttg acttggtcca aacgaacgat atagtcaatg
     8401 gatggattga gtgtgactgt ataaatcata cttctattac ctccgttttc tccttaataa
     8461 cctgcaagag ctcatgccct tgactagtga taacgatagc gcgtttgagt ggggctacct
     8521 tggcaaagca agtttgtcca atttttgacg aatccaccaa gacgtaggtc tgcttggcat
     8581 tctccaaaat agctcttttc acagctccct cctccatatc aggagtcgta taatagccat
     8641 cgtcaacacc attcattccg ataaaggcac ggtcaaagtg caattggtta atctggttaa
     8701 gagcaacgcc cccgatacta gcatctgtcg ccgtcttgac gtttcctcca accatgacag
     8761 ttggaatctg cttttcaacc aactgagcgg catggtgaat ggagttggtc acaactgtaa
     8821 cattcttatt gaccaattca tgaatcaaaa aagcagttgt tgttccagca tcgataaaga
     8881 tgacatcttt ttctttaatg agagaggctg ctttctgagc cagcaatttc ttttcttgaa
     8941 ggtttttgac agatttttct tgaatggttt cttcttcctg taaggagtgg gggagttctg
     9001 ctccaccatg cacacgacga agcttatttt ccgcttccaa ctcatccaag tctcttcgaa
     9061 ccgttgattc tgacgtttct agcaaactaa ctaatttttc tagagaaact acatgatgtt
     9121 gatttaactc ctctagaatc agttgtttac gttctgtttt taacactgaa tcacctcctg
     9181 ttatcgttta cactattcat tctaccatac tttatttcga agtcaatcat tttttatcat
     9241 tttctttcaa aatctatcat ttttcttatt tctagaattt ttattgcaag ataagagcct
     9301 ttatggtaga ctatttgagt aagtattgga agattactca agaggcttaa gaggccgtgt
     9361 tggaaacgcg gtaggcgtgt aatagcgtgc gtgggttcga atcccatgtc ttccgtcgaa
     9421 aaaagattat ttttaggatg caacaaatgt tgtatccttt tttaaaatag ggtctgtttg
     9481 tgactccctt tttagcaaaa gaatgcaaag gaataccaaa gataaaaata aaaaacgttg
     9541 atttaacaac gttttaccaa ggaatgcaaa agaatgcaaa ggaataatgg agccggtggg
     9601 agtcgaaccc acgtccaaac acctgccaac atatttgtct acaaccatag gttatgtatt
     9661 attttaacag cccctcgaca cataactcaa gtctagaaac tgcgagtcta tcaatctctt
     9721 atcaagccgc tagacaaggc ttaatcgtat ctcgctaata ataagacctg tcattgaaca
     9781 cgagcaatcc aaatcgggtc acgcctgctg gtttttaggc agctagagcg taagaagtgt
     9841 tattttttgc agttatattt aactgagcgt ttacgtcgcc acacgagtcg caaaatatgc
     9901 ctcataatgc ctgtcgaatc cgtaacgacc ccaaaagaca ataaaaccat tataccttat
     9961 ctaactcaaa aatgcaaaaa gaaatcacat acctaaaaaa gatactattc gagaaattta
    10021 gcaaactgga agactaacta caactttctc agtgcataac tttacgattg taaatagagc
    10081 ttacgaaatc caattaacac atacctaaga tgaagctatc tgttttgaag aaattcacga
    10141 agagaattat tctaaaatac tttcagatga gaatttt
//
LOCUS       DAVKAO010000061        10171 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKAO010000061 DAVKAO010000000
VERSION     DAVKAO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.061"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..220)
                     /locus_tag="VWX16_001620"
     CDS             complement(<1..220)
                     /locus_tag="VWX16_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158936.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFY"
     gene            complement(220..984)
                     /locus_tag="VWX16_001621"
     CDS             complement(220..984)
                     /locus_tag="VWX16_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158937.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1080..1483)
                     /locus_tag="VWX16_001622"
                     /pseudo
     CDS             complement(1080..1483)
                     /locus_tag="VWX16_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1663..2295)
                     /locus_tag="VWX16_001623"
     CDS             complement(1663..2295)
                     /locus_tag="VWX16_001623"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9158938.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2329..3030)
                     /gene="pyrF"
                     /locus_tag="VWX16_001624"
     CDS             complement(2329..3030)
                     /gene="pyrF"
                     /locus_tag="VWX16_001624"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9158939.1"
                     /translation="MREHRPIIALDFPNFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(3263..4462)
                     /gene="divIB"
                     /locus_tag="VWX16_001625"
     CDS             complement(3263..4462)
                     /gene="divIB"
                     /locus_tag="VWX16_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9158940.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     NQRSSRR"
     gene            complement(4472..5530)
                     /locus_tag="VWX16_001626"
     CDS             complement(4472..5530)
                     /locus_tag="VWX16_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9158941.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(5532..6872)
                     /locus_tag="VWX16_001627"
     CDS             complement(5532..6872)
                     /locus_tag="VWX16_001627"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9158942.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPGSHNV
                     ENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNILATQ
                     KALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKAGVAY
                     VEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE"
     gene            complement(6976..7617)
                     /locus_tag="VWX16_001628"
     CDS             complement(6976..7617)
                     /locus_tag="VWX16_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9158943.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7619..9640)
                     /locus_tag="VWX16_001629"
     CDS             complement(7619..9640)
                     /locus_tag="VWX16_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9158944.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMALTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLIDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(9711..10010)
                     /locus_tag="VWX16_001630"
     CDS             complement(9711..10010)
                     /locus_tag="VWX16_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9158945.1"
                     /translation="MKTKKHRLLALVLISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWDGHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
BASE COUNT         3078 a         2218 c         1807 g         3068 t
ORIGIN      
        1 aataaaaaaa gaataatata tatgtgaacg gaaagcaata tactccagtc gtcatatctt
       61 gaagtaaaac taagatccat tctaatacat ttggatggat tgaatattgg cgacagcgca
      121 ataaatatac tgtactagat aaaacacagg atagcagtaa tataaaataa accaatactg
      181 ataaaaaatc ttttttgtaa aattgaacaa attgtttcat tatacatagt cctctgaatg
      241 tagaaaaaat gtaccataaa caaccaaaca actaacaaat aaaataaaag caagatgccc
      301 actaactaag gaaagactga tatctttctg atatcccaaa gctaatgttg tcacaggttc
      361 taagtaagat agccctaaaa tagcccaaaa aataccacca accatcatat aggcaactgg
      421 gatgaaaata gctcctattt ttttcttcac tagcaaagca ctagctagtc caaaaataga
      481 gaacacagcg ccccaaactc cataccagag agtcgtcaca agactataga gcaactgatt
      541 agaatcaaat aattctttta aggcaccact ataatctcca atataaattt cctgataagg
      601 agtcactaaa agattaattc ctaataataa taaatagggg agaaaaaaga ctagaaaaga
      661 agaaataatt gcagtactac ctacaatagc cagatacttc tttttagaaa ttcggcacaa
      721 ttgtgctgtt agaaaatgac tctcagcatc ctctattatc tgactagaat agggcagtgt
      781 acagataagt gcagctacta ggctaatcgg tgaaaatcct cgaatagaag aagcggcaaa
      841 aaaaatcgat aaaccttcaa ttttataaat accattgaaa gcaaggaaat ttcctaaact
      901 catgcaaaga agggtcaaaa ataaagacat ataaaatcga ggtgattgaa cgactcgtac
      961 aagattacaa atgaaaaatt ccatccttac tcctccttat aataaaaata gggtgtagca
     1021 ttcttttttc atgctacacc cacaatcaac catctttaag gcttactctg acaagtaagt
     1081 taataagaat ctggactcca agaacctgaa gtatgaattc ttacatgatt tccaaattgt
     1141 ggcgccatag ctaatctagt accagaacca atataattgt caccacctcc attatagtac
     1201 atgacaatcc tagagccaga ccccaatgaa tataccgggg taatatctga cccactatag
     1261 gcgctacgaa tagaggtact taacctttta ccgccaccag tgctgtcact gttattaatt
     1321 ccagcagagg cgttttcttt ctcaagctca ctagttgtat ccgaacccca acgcggcaac
     1381 gttaaatcat aatttccaat ctgtctagct aaaactgtag ggagaaacaa actagatgca
     1441 attgtaacta cgcaaaaaag gaatatattt ttcttctttt catgacatcg tcctcttaag
     1501 aagtgataaa caagatagtt gattgatcaa tttgatacat attacatttt cattatatca
     1561 ataaaagaaa gcgttgtcaa ttattttatt taaaatattg tcatatttta aataaaatgg
     1621 taaaagagac tcggtagcct cgtctctctt tatcttactg acctaacctt cttgccaatt
     1681 ttcttggtct tctttaaagc gttttagaag atcaaggccc tctggcgtga tgtaaccttc
     1741 ttcttgggct agatggataa gctcgctata gtttgaaagc gtcacaagtt taacaccagc
     1801 atctgcaaag ttcttatctg cttttggcaa ttggtagctg aaaatcgcta caactccaag
     1861 tacatctgct ccttctcgct tggctgctgc tacagcttca agaactgaac cacccgttga
     1921 aataaggtct tcaaccacta ccattttttg accttgagct acgcgacctt cgatttgatt
     1981 accagctccg tggtcttttg gtttgctacg gatgtaggca aaaggcaagt ccatcttatc
     2041 agcaataatg gctccgtgtg gaatccctgc tgttgcagtt cctgcaatca cttctacttc
     2101 aggaaaggct tctttgatag cttccacaaa accattttca attagggtac gagtttctgg
     2161 ataggctagt gtcacacgat tatcagtgta aatcggtgac ttgataccag atgcccaagt
     2221 gaagggttcc tctggtttga ggtaaacggc ttggattttc aagaggtggc tagcgatatc
     2281 tttagcaagt gtcatggtat tctcctttta tttttctaat ctagttcttt aattccagtc
     2341 ctgtgtccat tcatccttga tggcatgata agctgcaaca ggatcctcag cttgggtaat
     2401 gggacgtccc actacgatat agtcactgcc gatttgatag gcatcagcag gtgtcatgac
     2461 tcgtttttga tctccaactg caacaccagc tggacgaatc cctggtgtca gacagataaa
     2521 atctggattg gtagcctgct tgatgacttg tacttcctga gccgagcaaa caacaccatc
     2581 caagccagct tcagctgtct tcttggcata gtgaatcaca gactcttgca gactggtttg
     2641 gatattttga aactcctgca tctgagcttc tgacgttgat gtcagctgag ttacagcgat
     2701 caatttggct tgactcccaa gaccttcacg cgccgccttc atcatctcta caccaccagc
     2761 cgcatggaca ttagtcatat cgacaccaag ctgagacaag accttcatgg ctgacttgac
     2821 tgtattagga atgtcatgaa gtttgagatc caaaaagaca ctatgaccca aaccttttaa
     2881 gtaggacaca atctcaggcc ccgctgcgta ataaagctcc atccctacct tgagataaag
     2941 gctttcttct gctgggaaaa gagctaaaaa ttccttgacc gcctcaaaat taggaaaatc
     3001 aagagcaatg attggacgat gttctcgcat aggtttctcc ttttgccttt actagtgaga
     3061 gagtctggcc aacagaagcc acgaaaaaag gaacctgcca acagcaaggt tccacgaaaa
     3121 atgggtagtc tccacccttt caccgtctaa ccttagctgc ctctctggac tgctttaaag
     3181 gttttttact attctattat ttctactagc acttgtcaag taaaaacatg gtcactaaag
     3241 aactataaga gaaaaggtaa acctagcgac gcgatgaacg ctgatttgtt tgatttcgat
     3301 tgctctcttc ctcttgtttt ttctgttctt cttcctgttt tttctcggct tccttggcct
     3361 cttgtttggc tttttcctca gcttccataa ttaatttatc cgccacagtg taactgtaaa
     3421 ttccagcttc catgtcgacc acactcggtt ctgacaattg tggcttaatc ttactgtaat
     3481 atggcagttt cttactcatt tcagatagag gaaccaagac ttcgtccgaa tcattcatgg
     3541 tcaatcgaat taaatcggat gtcaccttgc ttggggctaa ttccaccttt tggatagccg
     3601 ccttgagttc tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt tgttcactat
     3661 cattaaagag aactgataaa taagtttctg gtaaactgtt cagactcaca gaactagtct
     3721 caagctgacc actggaaaga ataggataat gattttcacc agaaatatag taggccacaa
     3781 tatcatattc cttgacctta atagtgaact tagttggaaa ttgatagaca agttgagctg
     3841 attcaaccca atagttagac ttaatctgct tttcatattt tgccttgtct agcagaaggt
     3901 taatcgtata atccgaatcc tgaatgcctg aagcctgtcg aatatcatca gctgtagttt
     3961 gcaccgttcc ctcaacacga atatctttca tggtcgcata aggactgagc aagtaggcag
     4021 agacaatcaa taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag atatggatac
     4081 caggaatctt tgctttggct ggtttttcct ttgtagcctt tttagaaagc tttttatcct
     4141 gttcctcctt ctctttagac tctggttctt ctttctcttc tttctctttg tcagcctctg
     4201 aggatgctac tttttcttca gactcttcct tagctgattc tgaatcttcc tggtctgttt
     4261 cactctcctg gtcctgtttg tcctctgact tctcagattc ttctcccatt cgagcttgtc
     4321 tttccttttc cttctcctca gctagagccg cctcttcttc agccttcttt tttagatatt
     4381 cttggtttcg tttctgccat tctgataact ctttcaattc ttcgaggatt tctttgtcct
     4441 catttttctt atcttttgac atttactttc cttatgataa atcttttttc aacaattgat
     4501 aaaaatctgc tagagatttc aattccttag aagccttcat cttagcttgg taatcttcct
     4561 tgtgacttag taagtgagaa agcttctctt ccaaactatc caaggtcaaa tcgctttctt
     4621 gaaggtcttc tgcatagcct ttttttacaa agtaagctgc attttcaagc tggtcaccac
     4681 gactagcttc acgaccaagc ggcacaatga catgcaattt tgctatcgcc aagagctcaa
     4741 aaatcgtatt ggcaccacct cgtgtcacaa caatatcagc caattccatc aagggttgat
     4801 agagatcggt cacatagtca acacgaaaaa gattttggct caactcattc aggctagaat
     4861 ctccagttag attgataata ttgtagcgct ctgttagttc tttcttatgg tctgtcacca
     4921 attggttaaa gacacgagca cctgcagaac cgccaacaaa caatacagtc ggcaatttat
     4981 gattaaagtg gctttgaata tccaccaatt catctggttc tggatttttt tgatctgaaa
     5041 ccttggtcac tgctcccaca tgctcaacct tagacaaact cgaagcttgc tcaaaggttg
     5101 aatacatctt agtcgcaaat ttataggcga ttttattggc caagcccata gacaggtcag
     5161 attcgtgaat aaagacaggc actcctgaca cacgcgcagc gataacaggc ggtactgaga
     5221 caaagccccc ctttgaaaaa agggtctgtg gacgaagtcg caacatgata aagagcgatt
     5281 ggacgattcc ccagcaaact ttgaagacgt ccagcatatt ttgccaagag aaatagcgac
     5341 gcaattttcc agtcgcaata gaatggaagg tgacatccaa acctgactta aggatttctt
     5401 ggtgttcgat accacgcttg tccccgatat agtggacttc ccaaccatct tcgatgaact
     5461 tgggcattaa caaaagattg agggtaacgt gtccaaccgt ccccccacct gtaaagacaa
     5521 tttttttcat attattcttt taactccgct actgtgtcga taaagaggtc gccacgtact
     5581 tcaaagttag catacatatc ccagctggca ttggcaggac taagaagaac cacatctcct
     5641 tgagtcgcaa gctcataggc cttgcgggtc gcatctgcaa tatctgtcgc ctccacataa
     5701 gcgacaccag ccttgtctgc tgcccgtttg acacgttctg cagattgacc caggatgacc
     5761 atcttcttga gtccagtaat gtctggcacc aattcgtcaa actcattgcc acggtccaaa
     5821 ccacctgcaa tcaagacgac cttgctgttg tcaaatcctg acaaggcttt ttgagtagcc
     5881 aagatattag ttgatttact gtcgttatag aatttaacac ccttgatgtc atccacaaac
     5941 tggagacggt gtttgacacc accgaaggct gaaagagttt ccttgatggt ttgattgtcc
     6001 acaccacgaa gcttggctac agcaatagtc gcaagggcat tttccacatt gtggctacct
     6061 ggaacaccga tttcattcgc tgccatgact acttcaccac ggaagtagag ttgaccatct
     6121 tccagataag ctccatcaac cttttcaagt gttgaaaatg gtacaacagt ggcttctgtc
     6181 ttggaagtca agtcttgatt aaagttcaag acaaggaaat cagctgctgt catcttgttc
     6241 tggatattcc acttggctgc tacatattcc gaaaatgacc catggtagtc gatatgagtt
     6301 ggcataaggt tggtaataac cgcaatctct ggatgaaatt cttgaacacc catgagttgg
     6361 aaagaagaaa gttccataac aagcgtgtcc ttgtccgacg cagtttgggc cacttgacta
     6421 gctggatagc cgatattccc tgataaaaga ccatgttgcc cagcagcagt caaaacttcc
     6481 ccaatcatag tcgttgtggt tgtcttaccg ttcgatcctg tgataccaat aatcggtgct
     6541 tctgaaatca aataagccaa ttccacctca gtcaagactg gaattccctt ggccaaagcc
     6601 ttttcaatca tgggattgtt gtaggggata cctggatttt tcaccataag agcaaactct
     6661 tcatccaaga gttccaaagg atggccacct gtaatgacct tgatcccttc ttccagcaaa
     6721 ctttgggcag ctggattgtc ctcgaaaggt ttcccatcat ttactgtcac aatggcacct
     6781 agcttgtcca acaaacgagc tgcagattca ccagacttgg ccaaacctaa aacaaggact
     6841 ttcttatttt taaattgatc tattactttc atgtctcgaa ctccatttct actcctacta
     6901 ttttaccatt tttatggaaa taaaaaagcc acaaagtgtg tttgtgactc tttcttctaa
     6961 ctgaatcttg ccttatcatc tatgtgataa atcggtaact cgaataactt ggtccacttg
     7021 ttcccaaata agaggattgt gggtcgcaat aataatagtt cgattcggat tttttaaaga
     7081 ttctaggatg gaaagtaatt cctcagagtt tttggggtct aaggaagcgg ttggttcatc
     7141 tgcgaggatc aaaggtggat cctttaaaat tatcttcgct agtgcaacac gttgtgcttc
     7201 tcctcctgat aactcaaata taggttgctt caaatccaaa taagagaggt ttacacggtt
     7261 tagagcttgt ttcatcaaag agattttctc tttttccttc aactttttac caactaaacc
     7321 cagattgaga ttctctttga cggtttggct ttcaattaag ccaaaatctt gaaataagta
     7381 tcctaagtaa tctctaaaga aaacagaagg cttgatgtcc ttaagagaag tgccatcata
     7441 gatgatttgc cctttgtcat atggctctaa tcgtccaatc atattcaaga gtgttgtctt
     7501 accacagcca cttgtaccga ttaaggcata aattttccca ccttcaaaat gaagattcgt
     7561 atctgaaaat agctgacggc ttccaaattt tttagatata ttctttagtt caatcatcct
     7621 attttccttt cataattgtc atagaaacac gagattcttt ctgcgcttga cggtaaagcg
     7681 tcagaactgc actagctaga aagactaata aagtgagcaa gccaatcacc aagtctcgac
     7741 tgcttaaaat aaagagacta gcaccaaata caaaactggc aaattgacta accatatact
     7801 gagcatgtgt ttcaaaaaat cgtaaacctg aaattcgttt aatcaagata tctcggcgga
     7861 attgctcgaa atatagaaga ttgacagaat aaaagagtaa caaggaacta gctatcccca
     7921 caatagctcc taagattaaa gttgctgttt cagtttgaac ttcattataa cgagttagat
     7981 aaacacttct tccttcttta agataggata cttgctcata aattccagct ttcttcaaga
     8041 gttctagccc actctcatat cctttgataa agagttgttt tccagcattg atagaccaac
     8101 tagataagga tataaaacta tcacctgtag aagtcggcgt gaatacaact aaaatcggat
     8161 caatcaaata ctgagtggat acgggattct caccgttatt ataaacaaac cgcttttctc
     8221 ccattgaaag ataactaacg tgcgctttca tctcataatc caaaggagca cttgcctcct
     8281 caccagattt tccataataa ctcaatcttt cttcaaaaac tttcttaagt tctgcttctc
     8341 gagagcgcaa atgttctggg agcaagagga taaactcacc tttttggaga tgggctaact
     8401 tctgtttggt ctcagcatct accacgacct tttccttgtc caaataactg ggactaacat
     8461 agagcgtatt agcatctgga ctataggtat ccagtgtctc tccctgttca ttttttcctt
     8521 gtggattggc aaaatggagc agattatcct ttacataaag agcttgttct tcttcgattg
     8581 cttccttggc aaaggcatac cacttgctct gattttctgt atcttttcct ctatcaccta
     8641 agccaaaaga aatttgataa tagtctgctc tatcctgcca tgcttgtttt gaaatttcaa
     8701 gttctttcaa tcgttggtaa gacatcaaac ctgtcttaac agcgtagcct actgtaaaga
     8761 cagcaaccaa ctgacacaat agggttaaag ccatcaagcg tttaaggggt aatctcccct
     8821 taataacagg aactaatgct ttgtaactca aactcattag gtaaaggagc attagtaaaa
     8881 ttgaaatcgc caataaaaac aacagataga aactaatccc aaaaccatag gtggctaaca
     8941 agataggata aaacaaacct tgactaaaaa gaacgactcc cccacctagg aaggaaagga
     9001 gggctgatag aaggagccat ttgatatcag tagataaaga atgccccatg atggataaga
     9061 gagtctgacc agaaaagagt tttatacctg ctgctctcat ttccttaatc cgagtgataa
     9121 tcactaaagc aaagaaagat aagccaaata ttgctaaact aattaaaata aggggattta
     9181 gtaatattcg aaaagcaaga aaatagggcg gtatctttcg gtcagcactt gctttataac
     9241 ccaaatctcc taatttatcg gcaagctttt ctttcgtcaa ggagcctgac aaaaggagat
     9301 aactatttag cggatcacta cgttcacgac tttcttggct agcttcttgg aattcttttg
     9361 gtaaagttcc ctgaccataa gttgcataag taaagtgagt cgtcccatcc ttactcggct
     9421 ctacaattct tctagctatt aaactctgtt ctgagtttgc aaaattctcc aattcctgtt
     9481 caaatacctc acgcgtcggt tcctgagtat cttttttgac acgaagtaaa gaaacggaat
     9541 catagcttgc atataaatat tgtggcgcac gtaagacaat aatccaagca aggaagaagc
     9601 tgagaaaaaa agttgataat aatatgaata gtttcttcat agtagactcc ttgtaaacaa
     9661 aattccccct gtaatttctt acaaggggaa cgatttaaat caatgaacga ttagtcataa
     9721 tcacagtaaa atgctacttg ttctccccat ttagtccaaa tccatgcata ggaggttttt
     9781 ccagcaggag cttctccttt atcagaatga ccatcccatc tgctcacgat ggatgaataa
     9841 tgatattttt taccatgata gtaatttgaa aaagcccaac cacctcctga accttctcca
     9901 tatgtccata ctcctccatc tggatattgt acagcagctg atgcagctcc caataatgta
     9961 aaacttgaaa taagaactag agcaagtaat ctatgttttt tcgttttcat tttatttttt
    10021 ctttcaaaaa aagcacacct tgagcaacaa tgcaacaaaa taaaccctcc tctttctttt
    10081 gttgaaaccg ctttcttatg tgatgaggat aacattttta ttatttgttg tcaaggaaaa
    10141 aatcgaattt tttagatatt ttactatatt a
//
LOCUS       DAVKAO010000062         9757 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKAO010000062 DAVKAO010000000
VERSION     DAVKAO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.062"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            268..363
                     /locus_tag="VWX16_001631"
                     /pseudo
     CDS             268..363
                     /locus_tag="VWX16_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(616..1236)
                     /locus_tag="VWX16_001632"
     CDS             complement(616..1236)
                     /locus_tag="VWX16_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9158946.1"
                     /translation="MKLYFVRHGRSLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1236..1718)
                     /locus_tag="VWX16_001633"
     CDS             complement(1236..1718)
                     /locus_tag="VWX16_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9158947.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMILQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1718..2560)
                     /locus_tag="VWX16_001634"
     CDS             complement(1718..2560)
                     /locus_tag="VWX16_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9158948.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2655..3455)
                     /locus_tag="VWX16_001635"
     CDS             complement(2655..3455)
                     /locus_tag="VWX16_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9158949.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3599..4978
                     /gene="glmU"
                     /locus_tag="VWX16_001636"
     CDS             3599..4978
                     /gene="glmU"
                     /locus_tag="VWX16_001636"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9158950.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGSCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4988..5533
                     /locus_tag="VWX16_001637"
     CDS             4988..5533
                     /locus_tag="VWX16_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9158951.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5545..5859
                     /locus_tag="VWX16_001638"
     CDS             5545..5859
                     /locus_tag="VWX16_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9158952.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5876..6568
                     /locus_tag="VWX16_001639"
     CDS             5876..6568
                     /locus_tag="VWX16_001639"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9158953.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            6645..7136
                     /gene="rocS"
                     /locus_tag="VWX16_001640"
     CDS             6645..7136
                     /gene="rocS"
                     /locus_tag="VWX16_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9158954.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7234..7824
                     /locus_tag="VWX16_001641"
     CDS             7234..7824
                     /locus_tag="VWX16_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9158955.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7833..9596
                     /locus_tag="VWX16_001642"
     CDS             7833..9596
                     /locus_tag="VWX16_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9158956.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3001 a         1696 c         2085 g         2975 t
ORIGIN      
        1 cccaaaagtt agattttttc tgtctaactt ttggggtgta gttcatgtac acctgatatg
       61 atgcgtttta taattttaaa gactttttga ccagcctcat ttttttaact tgatactcag
      121 tgaaaagcaa agattaaact aggaagctag ctgtaggctg ctcaaagaac agctttgagg
      181 ttgcagataa aacttgtgag gtaaccaaca tatataatgt gaagctgacg tggtttgaat
      241 agattttaga agggtatgag tctggaagtt ttaatggata atgcaagatt ccatagaatg
      301 ggtaagctag agttcttatg tgaagagttt gggcataaac ttttaccttt tcctccctac
      361 tcatcttagt atagaaaagt gaatctaaaa tagtacataa ctgcttctaa aacattctta
      421 taaattgatt taaattctca aatcatatta ttcagttctt atttcatttt gctctacaat
      481 cctgttgaga agacacgtgt tcatatcaaa aaggtattgg caagttgcaa taccttttta
      541 caaggttctt ttgtcttatt tttgtttcaa ctgactatat ctcctatggt tctagttcag
      601 aaggctaggc tataattatg attgataaga agtatcattc caagtattga gagtgaatgt
      661 ttcaaaatca tgggtttcta taatggtcag gctggcattt gctagaccgc catctttacg
      721 aagaagtggt tctttatagc ctaggagagt acgaagactg gcagtaagat tggcgccgtg
      781 tccgacaatt agaatacgct cagctggact atcttttaat gatttgataa attggatggt
      841 ccgctgagtt gtactataga gggattcggc tccgaacatt cgagtgtcaa attgagcaag
      901 atttgaacga aaagcctgga tttgttgcgg gtaaatagct tccagggttg caattttcaa
      961 accttctaac ttcccaagtt gccattcacg gagattagga acgatttcta aagaacaggg
     1021 ggtatggagt tgactttgga taatctcagc agatttgacc gctcgaggta aatcacttga
     1081 ataaatctga tcaaaaggaa tttccttgag atactggcca agtcgtttta gggtttcaat
     1141 ggattcagga agaagaggag aatcaccgct agcaccttga aaacgacctt cttggttcca
     1201 gaggctacga ccgtggcgga caaagtagag tttcattact tgatgtcctc caaaatatct
     1261 acaaagtctg cctttacaaa gctagccaag tcttgtggcg cgacgataat gctgtgtccg
     1321 acttcgcctg cagagacaat cattcgatcc aaatctagag caattttatc gataaaaatg
     1381 ggataattgt gtttctgacg aattccgaca ggattattgg ctccatgaat gtaaccagtt
     1441 gttttttcta agtccttttg tagaatcatg ctcacttttt tattgccaga aattttagct
     1501 agtttctttt cagacaagtg ctgagtgata gggacaattc cgataatcgg tccggtcttg
     1561 tctcccaaaa gcgccaaggt tttgaaaatc tgatctcgtt cataaccttg aggaagctct
     1621 ccttctaggg cattgatttg aatcccctga tgagggatgg ctgctttaga taggatttgt
     1681 tccaccaatg tttttttgat tttaactttt tttgccatta tttatattta tcctccaatt
     1741 gactcatcca aataccaagc cagattccca gcgcaaagaa gaaggcgatg atgacataac
     1801 cgacaagtga aagtcctgtg tattggatac tttcagcgtt tcctgcattt ggaattaaga
     1861 tcaaaagggt acttgatagg acgataccga tgatgaaatg atagacgcgt gagtggtagt
     1921 tgtttaaggc ataatccatc aattttgaaa aaacgatgag agttgcacct gcaccaattc
     1981 caatcggaaa gaaggttccc aagagatcaa aagttttaaa accagtcaac ataggagcat
     2041 agagtcccaa aatcaaaagt aaattcgatg ggctgaggcc aggaaccaag acgccaaggg
     2101 ccaatagtgc gcctgctagg acgaagttaa gaaagctggc gcttaaggtt ccaacgacaa
     2161 aatttaaggc atatagtcct aatccagaaa tgataaaggt tgtccataac caagctaaat
     2221 caatcttgtc tcggtcagat tctcgagttg attctttgag gaggctagga actgtaccga
     2281 taatagctcc cgcaaagctc cataatacaa agacctgata attttcaagc aggtattcaa
     2341 tcgggtagga aaataagccg attcccagaa gcataccgat ggcaactgga ataaagtaca
     2401 aaacattttc tttaaggtct ttaaagggat gggccagaaa gccaatcatt cgttcataga
     2461 ttcctaagat tgctgctaga acccctccgg aaattcccgg taggataaat ccaagagcaa
     2521 ttacaatccc tttaataacg cgtgctaacc atgagagcat atatttcctc caactatttc
     2581 tatttcaaaa aatccttact atattgtatc acaatcagac acaaaaagaa aaagcaaatg
     2641 ataaacaaat gcttttaagg ttttaaaaaa agctttcgaa aggtttcttc tttatttttt
     2701 aagggagaga taacgttgat atctaaatcg tggtcaaagc cggcaatttt tcctttagat
     2761 gtgtattggt gaatatcata atctaaatca gttttaggac tgctctccaa aaatcctgag
     2821 tctgagccgt aggacggaat ccaaacagag gtaaacttgc ctgtatcaat actgtgttct
     2881 tccatgaagt agacaccaac gtagatgccg atgtttttag cacctagtga tgctagtttt
     2941 gctcgaaagt tttcgacacc ttcgttcata ttagacatag ttttgtcttc cacgtcaagc
     3001 caatagtaac tagggctgta aggagaggca gcattgtaga aaacttcggc agccttttcc
     3061 atttcttgga cactttttcc agctacataa gcgtagacag caactgggac attccgcttt
     3121 tgaagttcag tgatatgact cttataggcc ttgtctattc cattgataaa tgaagcatca
     3181 ttttcttttg tcgtttgagc accactgtga acacgaacaa tagcacctga aatattttgt
     3241 gagagggcat cgtagttgat ttcctcagga cgctgccagc cagagaggtc aataatcggt
     3301 ttgtctaagt gtttcaaagc ctgtgcttca atctgtgcta tattggattt tgttttaaac
     3361 gattggctgt cattaagtgg gcgattgatg attaaaatga acatcataat cccaaaaaaa
     3421 ctaaataaaa taagtggatg aatttgtttt ctcatatctt ataattctac cctaaaaatc
     3481 aaaaaaaatc aaaaaaatgg gttaaggaag agactttaga gcattttttc attcaagagt
     3541 gcggaatgat ttgaaatatg gtataataaa agggaatttc tacagaaaag agaagattat
     3601 gtcaaatttt gccattattt tagcagcggg taaagggact cgcatgaaat ctgatttgcc
     3661 aaaagttttg cacaaggttg cgggtatttc tatgttggaa catgttttcc gtagtgtggg
     3721 agctatccaa cctgaaaaga cagtaacagt tgtaggacac aaggcagaat tggttgagga
     3781 ggtcttggct ggacagacag aatttgtgac tcaatctgaa cagttgggaa ctggtcatgc
     3841 agttatgatg acagagccta tcttagaagg tttgtcagga cacaccttgg tcattgcagg
     3901 agatactcct ttaatcactg gtgaaagctt gaaaaacttg attgatttcc atatcaatca
     3961 taaaaatgtg gccactatct tgactgctga aacggataat ccttttggct atggacgaat
     4021 tgttcgtaat gacaatgctg aggttcttcg tattgttgag cagaaggatg ctacagattt
     4081 tgaaaagcaa atcaaggaaa tcaacactgg aacatacgtc tttgacaacg agcgtttgtt
     4141 tgaggctttg aaaaatatca ataccaataa cgctcaaggc gaatactata ttacagacgt
     4201 cattggtatt ttccgtgaaa ctggtgaaaa agttggcgct tatactttga aagattttga
     4261 tgaaagtctt ggggtaaatg accgtgtggc gcttgcgaca gctgagtcag ttatgcgtcg
     4321 tcgcatcaat cataaacaca tggtcaacgg tgttagcttt gtcaatccag aagcaactta
     4381 tatcgatatt gatgttgaga ttgcttcgga agttcaaatc gaagccaatg ttaccttgaa
     4441 agggcaaacg aaaattggtg ctgagactgt tttgacaaac ggtacttatg tagtggacag
     4501 cactatcgga gcaggagcgg tcattaccaa ttctatgatt gaggaaagta gtgttgcaga
     4561 cggtgtgaca gtcggtcctt atgctcacat tcgtccaaat tcaagtctgg gtgcccaagt
     4621 tcatattggt aactttgttg aggtgaaagg atcttcaatc ggtgagaata ccaaggctgg
     4681 tcatttgact tatatcggaa gctgtgaagt gggaagcaac gttaatttcg gtgctggaac
     4741 tattacagtc aactatgacg gcaaaaacaa atacaagaca gtcattggag acaatgtctt
     4801 tgttggttca aattcaacca ttattgcacc agtagaactt ggtgacaatt ccctcgttgg
     4861 tgctggttca actattacta aagacgtgcc agcagatgct attgctattg gtcgcggtcg
     4921 tcagatcaat aaagacgaat atgcaacacg tcttcctcat catcctaaga accagtagga
     4981 gcctatcatg gagtttgaag aaaaaacgct tagccgaaaa gaaatctatc aaggaccaat
     5041 atttaaactg gtccaagatc aggttgaatt accagaaggc aagggaactg cccaacggga
     5101 tttgattttc cacaatgggg ctgtctgtgt tttagcagta acggatgaac aaaaacttat
     5161 cttggtcaag cagtaccgca aagctatcga ggctgtctct tacgaaattc cagccggaaa
     5221 attggaagta ggagaaaaca cagcccctgt ggcagctgcc cttcgtgaat tagaggaaga
     5281 aacagcctat acagggaaat tagaactctt gtacgatttt tattcagcta ttggcttttg
     5341 taatgagaag ttaaaactat atttagcaag cgatttgaca aaagtggaaa atccgcgtcc
     5401 gcaggatgag gatgaaacct tggaagtcct tgaagtgagc ttagaagaag cgaaagaatt
     5461 aatccaatca ggtcatattt gtgatgccaa gacaattatg gctgttcagt attgggagtt
     5521 gcagaaaaaa tagaggaggt cagtatgggt aaatctttat taacggatga aatgattgaa
     5581 agagctaata gaggcgaaaa aatttcaggt cctcctttgc tagatgataa tgaggaaact
     5641 aagattttac caacctcttc ttcccgtttt ggttatgcca atcctaagga tcatggtttt
     5701 agccaggaaa ccttgaagat tcaggtcgaa ccatctattc ataaaagccg tcgtattgaa
     5761 aataccaaga gaaatgtctt caattctaag ttgaataaaa tcttatttgc ggtcatcttt
     5821 ctcttgattt tgcttgtttt agcaatgaaa cttttgtaat agaaaaggaa ttgaaatgaa
     5881 aataggaatt attgctgcta tgccagaaga actggcttat ctggtccagc atttagataa
     5941 tgcccaggag caagttgttt tggggaatac ctatcataca ggaaccattg cttctcatga
     6001 agtcgttctt gtagaaagtg gaattggtaa ggtcatgtct gctatgagtg tggcgatttt
     6061 ggctgatcat ttccaggtgg atgcccttat taatacgggt tcagctgggg cagtagcaga
     6121 aggtatcgct gttggggatg tcgtgattgc tgacaaatta gcctatcatg acgtggatgt
     6181 cacagctttt ggctatgctt atggacaaat ggcgcaacaa ccgctttatt tcgaatcaga
     6241 caaaaccttt gttgctcaaa tccaagagag tttatctcaa ttggaccaaa actggcatct
     6301 tggtttgatt gctacaggag atagttttgt tgcaggaaat gacaagatag aagcgattaa
     6361 gtcccatttc ccagaagttt tagccgtgga gatggagggg gcagctattg ctcaagcagc
     6421 gcatgccctc aatctcccag tcttagtcat ccgagctatg agtgacaatg ccaaccatga
     6481 agcaaacatc ttttttgatg agtttattat cgaagctgga cgtcgctctg cccaagtctt
     6541 gttggccttt ttgaaggctt tagattaagc ggaaatttga cagtttttct agcttatgat
     6601 aagatttaag taaagaaaag ctagaaaacg tttcagagga tattatgagt attgaaatga
     6661 ccgtcagtga gattgcagag gtcttaggat tatctcgcca agcaatcaat aaccgtgtca
     6721 aagaattacc agaagaagac acagataaaa atgacaaagg ggtaacagtt gttaccagaa
     6781 gtggcttgat taagctagaa gaaatctata aaaaaacgat ttttgaagat gagcctgtca
     6841 gtgaagatgt caaacaacgt gaactgatgg agattctagt ggatgagaag aatgcagaaa
     6901 ttcttcgtct ttatgaacaa ttaaaagcca aggatcgtca gttatcagaa aaagacgagc
     6961 agatgcgtat caaagaccgt cagattgctg agaaagacaa acaattagat cagcaacaac
     7021 aattgaccct tcaagccatg aaggatcaag aaaatcttaa gctagagctg gaccaagcaa
     7081 aagaagaagt ccaatccact aagaaaggct tttttgctcg tttatttgga ggataatcaa
     7141 ggagctgttt aggttaaatg ctaacctgat ggcttctttt gtttgtgaat agtatagttg
     7201 attagaacag agacagtaga ggagaggtac agaatggaac gattagaaga ttacattgct
     7261 tttgacttag aatttaatca gcacgaagga caaacacact tgattcaagt atcggcagtg
     7321 cgttttagag atggtcggga aatcgatgcc tatgattcct atgttcatac cagcgtccct
     7381 ctaaaaagct ttatcaatgg tttgaccggg attacagctg agaccttaaa agatgctcct
     7441 ccagttgaga aagtcttaga agaattccaa gagtttgtgg gttctttgct gatgataggc
     7501 tacaatgctg ctaaaagtga tttacctatt ttagcagagc atggtttaga ctatagccaa
     7561 cagtacaagg tggatttgta tgatgaagct tttgaacgtc ggagttcaga tttacatggt
     7621 attgccaatc tcaaattgca aactgttgcc tcatttcttg gatttcaagg tcagtctcat
     7681 aatagtttag aggatgctcg gatgacggcg cgtgtttacg aatccttctt agagtcggat
     7741 caagctaggg aattgttaga gacagaaagt agcttatcaa acaatccttt tggtggcttg
     7801 gacttgtctc aattatttga ctaggagtgc ctatgaaacc tgaaaaacct aaaaaattgg
     7861 gtcttaaaca gatttacctc aatcttgata aaatcctctt tctatttttc ttgattttta
     7921 tgatagtgga gtatttgtgg gtaccattca attcatggtt ggctagcttc ttattagatc
     7981 agacaggtta tctttttatt tcctataaca atgtcttagc catccttact cattctccat
     8041 tgattagtct agctttttta gccttgattg taattaacct tctggttgct tattttcaaa
     8101 taggactcct ctttattggt gctcgtcatc tcctctataa tgaaaagcga aggctaattg
     8161 agtatatccg caaggttttc catgaaagtt ttggatttat gaaaaagcta acttgcgcta
     8221 aagccttgtt tatctttttc tatatagcta tgctttttcc ttttatacgg aaaatgttga
     8281 agatttatta tcttaataaa atcgtcattc cagagtttat ccaagcttat ttagaagata
     8341 aatattggat gtggtggctg agcatcctac ttctatctct tatctttctc tatgtctctg
     8401 tcagattgat gtttgccctt ccgaagattg tatatgatca gctgactgtt cgagaagcag
     8461 tgatgtttag cttggagaaa accaagaaga gagttacttt ttatgcttgg aatttatttt
     8521 acatcttgct caaagcacat ttattatttt atcttccttt gattccttta ttattggctc
     8581 aaactttggt agatgatatc actcagagag agtctctgat tcttggtatt ttgaattttg
     8641 tcgtgattaa aaatctctat tatatggctc tgacttattt tttggttaaa tttgtttcat
     8701 ttttgacagg aaaggagctg gatatgcttc ctaggagaga aaaagatcac atcgtgcgat
     8761 ggggtgtcat gacttgtgcc agtcttttct ttgccttaga aggttatatt tatctggagg
     8821 ctcccatggt tcatctacct caacttattt ctcaccgagg ggtttccaat gcaaatggga
     8881 ttcaaaatac agtagagtcc ttggaaacta cagcacaact caaaccagac ttggtggaga
     8941 cggacgtgca ggaaacaaaa gatgggcagt ttgtcatgat gcatgatgct aacttgaaaa
     9001 atttagcagg tatcaataaa agtcctcaag acttaaactt ggaggagctt aaagggattg
     9061 atatttttga aaatggctac cagactaaaa tttcaagctt tgaagattat ctcagtcgag
     9121 ccaacgaact tggtcaaaaa ttactaattg aaattaaaac cagtaaaaaa gatagtccag
     9181 acatgatgaa ccgcttttta gcccgttatg ctgcaaagct caagatttat ggacatcaaa
     9241 tccagtcttt agactaccat gttgtcgaaa aagtaagaca gtatgatgcc gaactgccag
     9301 tttatttcat catgccctac aattctgtct ttcctaaaac aagagcgaca ggatatacta
     9361 tggagtactc aaccttggat gaatattttg taagcaaact atggacaacg gatcagaaac
     9421 tttatgtctg gactgtcaat gattcagaag ctatcagcaa atctcttcac ttaggagtag
     9481 atggagtgat tacagatgat ttggaaaaag ttcagcggga aatagaagta gcccaagaag
     9541 atccagaata tacggatttg ctcttgaaaa aagcattgga attctttgaa ttttagtaat
     9601 aaaaaagagg aacggttgtt cctcccatga taggtaagta ttcttgtgaa tgcttgcttt
     9661 tttgtggttg caatccgttt acgagaattg tttgtattgt tcttgtttca atttacttta
     9721 tagtgctttg aagttggaat gctacactat aacttct
//
LOCUS       DAVKAO010000063         9611 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKAO010000063 DAVKAO010000000
VERSION     DAVKAO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.063"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(61..258)
                     /locus_tag="VWX16_001643"
     CDS             complement(61..258)
                     /locus_tag="VWX16_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9158957.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKHLHISPKELKLLGRIVK"
     gene            complement(333..1109)
                     /locus_tag="VWX16_001644"
     CDS             complement(333..1109)
                     /locus_tag="VWX16_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9158958.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            1231..1977
                     /locus_tag="VWX16_001645"
     CDS             1231..1977
                     /locus_tag="VWX16_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9158959.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYETKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRVIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     CQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1990..2970
                     /locus_tag="VWX16_001646"
     CDS             1990..2970
                     /locus_tag="VWX16_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9158960.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3165..3485
                     /locus_tag="VWX16_001647"
     CDS             3165..3485
                     /locus_tag="VWX16_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9158961.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3531..3839
                     /locus_tag="VWX16_001648"
     CDS             3531..3839
                     /locus_tag="VWX16_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9158962.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3856..5154
                     /locus_tag="VWX16_001649"
     CDS             3856..5154
                     /locus_tag="VWX16_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9158963.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            5305..7743
                     /locus_tag="VWX16_001650"
     CDS             5305..7743
                     /locus_tag="VWX16_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9158964.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7762..8430
                     /locus_tag="VWX16_001651"
     CDS             7762..8430
                     /locus_tag="VWX16_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9158965.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            8448..9536
                     /locus_tag="VWX16_001652"
     CDS             8448..9536
                     /locus_tag="VWX16_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9158966.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         3171 a         1346 c         1929 g         3165 t
ORIGIN      
        1 ttcacatctt atttcaattc actatattag agtaaaattc tctacaaaaa gaagaatagc
       61 ctattttact attcttccga gtaatttcaa ttcctttggg gaaatatgga gatgcttttt
      121 aaatactttg gcaaaatatt tatagtctga aaatccaact tggtcagaga tagcgcttaa
      181 aggggagttg ggagattcag ccatcttatc tatagcttgt ttcaggcgat attgtaagat
      241 atattcattt agagtcatgg ggaagtcttc tttgataacc ttatatagat gcttgttatc
      301 tgcaaatagc tagagcgttt caaggaaatg aactagaaat aacaattaat atggtggttc
      361 agaaatacct tttgataatc aataagatct tcaggatgaa gtgcgttgac tccatccatt
      421 tcatatttcc gatttaataa acgatatttg ttttcaccaa attgatgaaa agggagtagt
      481 tgaacttggt cgatatttaa tgagttaaat agagtagcga atttttctgc atcctctaaa
      541 ctattgttaa aattaggaat aactgggatt cttaaaacga tagttttatt ttgagaaaaa
      601 gcataatgaa tgtttttaat aatcatttga ttaaaaaccc cagtcacttt tttatgtttt
      661 atagaattat aatgttttag gtctgtgtag ataaaatcca catattgaat taaatcaata
      721 aatttttcat gatcaacaaa ggcagtagtt tcaatggcag tgtgtatgtg atgttcttta
      781 gctgatttta agatggcttt agcaaattca aactgagcaa atatttcacc tcctgataaa
      841 gttaaacctc caccggattc ttcgtaaaat tctttgtctt ttaatacctc tgtaataatt
      901 tcttctacag tcttttcttc accgactaag gtgaatttct ctcgttgagc atctttcatt
      961 ttttcaggtt tcattctttg agattcagga ttagaacacc atggacagcg cagaggacat
     1021 ccttttaaaa aaacagttgt acgaataccc ggaccgtcat gaattgaaaa gtgttgaata
     1081 ttaaaaataa ttcctttaga tatttccata agagttcctc ctcttcataa gtctatccta
     1141 tcacaaaaac gaaagaaaaa caattacgaa cgaaaggtta atttgttgtt ttttgctttt
     1201 tattttggta gaataaaacg gaggtgtaaa atgaaaagat tggaacaaat tattaaatta
     1261 gtatcagaat atgaaaagat cgacgttaat acattatcgg aaaaattaaa tgtatcgaaa
     1321 gtaacgatta gaaaagattt agataaatta gagtcaaaag gtttattaca cagagagcat
     1381 ggatatgctg tattaaatag tggagatgac ttaaatgtac gtttgtcaat taattatgaa
     1441 actaagagaa aaattgttca ggaagcagta aaattggtgt cagataatga aacaataatg
     1501 atagaatctg gatcgacctg tgctttactt gctgaggaaa tttgcaagca aaaaagaaat
     1561 gttacgattg taacaaattc gttttttata gcaaattttg tgagagctta tgattcatgt
     1621 cgtgttattg ttcttggtgg tgagtttcag aaagattcac aggtgactgt aggaccttta
     1681 ttaaaagaaa tgatacagac ttttcatgtg tgtcaagctt ttgttgggac agatggttac
     1741 gataaagaga tgggctttac cggaaaagat ttaatgcgca gtgaggtagt tcaatatatt
     1801 tcagcagtgt cggataaagt cattgtccta actgactcaa gtaaatttga taaaagaggt
     1861 acagtaagaa gatttgcttt aagtcaagtc tatgaagtaa taacagacga aaaactttct
     1921 aaacaaaata tagctacatt agaaaatgct gggataatgg ttaaggtagt ttcgtaagag
     1981 gttaagtgta tgaatcaaga taggaataaa ctgctttcta aaattgctta tctgtattat
     2041 attgaaaact taaatcagtc acaaatagca gcaaaattag gaatttatag aacctctatt
     2101 agtagaatgt taacagaagc aaggaatgta ggaattgtta aaattgaaat agagaatttt
     2161 gataccaata tgtttaagtt ggaaaattat gtaaaagaaa aatacagttt ggaaagttta
     2221 gaaattattc caaatgaatt tgatgatact ccaacaattt tatctgaaag aatttctcaa
     2281 gttgcagcag gcgtccttag gaatctaatt gatgataata tgaaaattgg cttttcttgg
     2341 gggaaaagtt taagtaattt agtagattta attcacagta aaagtgtccg aaatgttcac
     2401 ttctatcctc tagcaggtgg tcctagtcac atacacgcta aataccatgt gaatacactg
     2461 atttatgaaa tgtctagaaa atttcatgga gagtgtacat ttatgaatgc aacgattgtg
     2521 caagaaaata aattgttagc agatggtatt ttgcaatcaa gatattttga aaatttgaaa
     2581 aatagttgga aagatttaga tatagctgta gtcggaattg gtgattttag caataaaggg
     2641 aaacatcaat ggttagacat gcttacagag gatgacttta aagaattgac caaagttaaa
     2701 actgtaggag aaatttgttg tcgatttttt gattcaaaag gtaaagaagt ctatgaaaac
     2761 ttacaggaaa gaacgatagc catctctttg gaagatttaa aaaatattcc tcaaagttta
     2821 gctgttgctt acggtgatac gaaagtatct tcgattcttt ctgtcttgcg tgctaattta
     2881 gtaaatcatt tgattacaga taaaaataca attttaaaag ttttggaaga agatggtgat
     2941 ttgactttta gagagattct aggtgagtga aaatgataga ctgatcagtt tatcgttttt
     3001 tctttttagt cgattgcaca tttgtgctta tataaacaaa aatagtttat ctgttgtttt
     3061 tggattgaca actttattat gtagttgtat tctatagtta caaaagaaaa ttttaaaatt
     3121 tcaaatgaaa aaagcttttt acatagtgaa atgaggagga atttatggaa attattgttc
     3181 cagatcaaat tatcatgggt ttaattttat atgctggcga tgcgaaacaa catatttata
     3241 aagcattaga ttacataaag aacggtacat gtgaacgatg tgaagaagaa atccagttag
     3301 ctgatgccgc cttattagaa gctcataatc tacaaacaaa attcttggca caggaagcgt
     3361 ctggtacaaa gacagaaatt acagctctct ttgttcattc acaagatcat ctcatgacta
     3421 gtatgacgga gattaattta atcaaagaaa ttattagttt gagaaaagaa cttcataaaa
     3481 aataatacta gagtattatc attgttatta acatagagga ggaaaacata atggtgaaga
     3541 ttggtttgtt ttgtgcagca ggtttttcta ctggtatgct tgtaaataat atgaaaattg
     3601 cagcgcaagc tagtggagtt gaggcagaaa tagaggcgtt ttctcagtct aaattagcgg
     3661 attatgcgcc aaatatagat gttgcactat tgggtccaca agttgcttat acattagata
     3721 aatcaaaaga aatttgtgat aagtgtgatg ttccgatagc tgttattccg atgatggact
     3781 atggtatgtt agatgggaaa aaagtattag atttggccct atctttgatt agtgggtaag
     3841 aaaaggagat ttattatgtc aaagatggat gttcagaaaa tcattgcacc gatgatgaag
     3901 tttgtgaata tgcgtggcat tatagctcta aaagatggga tgttagcaat tttgccattg
     3961 acagtagttg gtagtttgtt cttgattatg ggacaattgc cgttcgaagg attaaataag
     4021 agcattgcta gtgtttttgg agctaattgg acagagccgt ttatgcaagt atattcagga
     4081 acttttgcta ttatgggtct aatttcttgt ttttcaattg cctattctta tgctaagaat
     4141 agcggagtag aggctttacc agctggagtt ctatctgtat ctgcattctt tattttgcta
     4201 agatcatctt atatccctaa acaaggtgag gcgattgggg acgctattag taaagtttgg
     4261 tttggaggcc aaggaattat cggtgctatc attataggtt tggtagtagg aagtatttat
     4321 accttcttta taaagagaaa aattgttatt aagatgccag aacaagttcc acaagctatt
     4381 gccaaacagt ttgaagcaat gattccagca tttgtaattt tcttatcttc tatgattgta
     4441 tatattttag cgaagtcatt gactaatggc ggaacattca tagaaatgat ttattctgct
     4501 attcaagttc cgttgcaagg tttaactgga tctttgtatg gtgctattgg aattgcattc
     4561 tttatatcat ttttgtggtg gtttggtgtt catgggcaat cggtagtaaa tggagtagtg
     4621 acagctctgc ttttatctaa tcttgatgct aataaagcta tgttagcctc tggtaatcta
     4681 tcattagaaa atggtgcaca tattgttact caacaatttt tagattcatt tttaattcta
     4741 tcaggttcag ggattacgtt tggtcttgta gttgccatgc tttttgcagc aaaatcaaaa
     4801 caataccaag ccttaggaaa agttgcagct tttccagcaa tatttaacgt aaatgagcca
     4861 gttgtatttg gatttccgat tgtcatgaat ccagttatgt ttgtaccttt cattcttgtt
     4921 cctgtacttg cagctgtgat agtatatgga gctattgcaa caggtttcat gcaaccattc
     4981 tcaggggtaa cattgccttg gagtacacca gctattctat caggattttt ggtgggtgga
     5041 tggcaaggtg ttgtgactca gttattagta ttggtgatat ctacagtggt ttactttcca
     5101 ttctttaaga tacaagatcg tttagcttac caaaatgaaa tcaaacaatc ttagaggtat
     5161 ttgtgtgtta ctgttaaact cacacatttg tgctaaaaat tagagagtta aaatttttct
     5221 agttaaaagc ttgaaaattt ctataaaaat cggtattata ttttcgaaag aaataaaaat
     5281 attttcgaaa gaaaggtgct tacgatggta aatacagaag tagcaagaac aacaatcaag
     5341 acagaatatt ttggcagcct tactgaaagg atgaacaaat atcgagaaga tgttttaaat
     5401 aaaaaacctt atattgatgc tgagagagca gttctagcaa cacgcgccta tgaacgatac
     5461 aaggaacaac ctaatgtcct aaaacgtgca tatatgctga aagaaatttt ggaaaatatg
     5521 tctatctata ttgaagaaga atctatgatt gcgggaaatc aagcttcttc caataaagat
     5581 gctcctattt ttccggaata tacgctagaa tttgttctca atgagttgga tctttttgaa
     5641 aagcgtgatg gagatgtttt ctatattaca gaagaaacaa aagaacaact tagaagtatt
     5701 gctccgtttt gggaaaataa taatttacgt gctagagctg gtgccttatt acctgaagaa
     5761 gtgtctgttt atatggaaac aggattcttc ggtatggaag gtaagatgaa ttctggagat
     5821 gctcacttag cagttaacta tcagaaactt ttgcaatttg gtttaagagg ttttgaagag
     5881 cgggctcgta aagcaaaagt agctctagat ttaacagatc cagcaagtat tgataaatat
     5941 catttttacg actctatatt tatcgtaatc gatgctatta aagtatatgc aaagcgcttt
     6001 gttgctcttg ctaaaagttt agccgaaaat gcaaatccta aacgtaagaa agaattactt
     6061 gagattgcag atatttgctc tagagtccca tatgaaccgg caactacttt tgcagaagct
     6121 attcaatcag tttggtttat tcaatgtatt ttacaaattg aatctaatgg ccactctctt
     6181 tcatatggcc gttttgatca atatatgtat ccatatatga aggctgattt agaaagtggt
     6241 aaagaaacag aagatagcat tgttgaacgt ctgacaaatc tttggattaa gacaattaca
     6301 attaataagg ttcgcagtca atcacataca ttttcttcag caggaagtcc tttatatcaa
     6361 aatgttacaa ttggtggaca gactcgagat aagaaggatg ctgttaaccc attatcttat
     6421 ttggtcttaa aatcagttgc acaaacccat ctaccgcaac ctaatctaac tgtacgttac
     6481 catgcaggtt tagatgctcg tttcatgaat gagtgtattg aagtgatgaa acttggtttt
     6541 ggtatgcctg catttaataa tgatgagatt attattcctt cttttattgc aaaaggagta
     6601 ttggaagatg atgcttatga ttacagtgcc attggatgtg ttgaaacggc agttccaggg
     6661 aaatggggct atcgttgcac aggtatgagt tatatgaact tccctaaggt tctacttatc
     6721 acgatgaatg atggaattga tccggcttcg ggtaaacggt ttgcaccaag ctttggtcat
     6781 tttaaggata tgaagaactt ttctgaatta gaaaatgctt gggataaaac actaagatat
     6841 ttgacacgaa tgagtgttat tgttgaaaat tctattgatt tatcattgga acgagaagtt
     6901 cctgatattc tatgttcagc attgactgat gattgtattg gtcgtggaaa acaccttaaa
     6961 gaaggtggag cagtatatga ttatatatca ggattgcaag ttggaattgc aaatttgtcg
     7021 gattcattag ctgcaattaa aaaattggtg tttgaggaag aacgtataag cccaagtcag
     7081 ctttggcatg cactggaaac agattatgcc ggagaagaag gtaaggtcat tcaagaaatg
     7141 ttgattcatg atgcacctaa gtatggtaat gatgatgatt atgctgacaa attggttact
     7201 gctgcttatg acatttatgt tgatgaaatt gctaaatatc ctaatacacg ttatggaaga
     7261 ggtccaattg gaggaattcg ttattcagga acatcttcta tctcagccaa cgtagggcag
     7321 ggacgtggaa cattagcaac tccagatgga cgcaacgcgg gtacaccgtt agcagagggt
     7381 tgttcaccat cacataatat ggatcaacac ggccctacat ctgttttaaa atctgtttca
     7441 aaattaccaa cagatgaaat cgtaggtggg gttctcttaa atcagaaagt aaatcctcaa
     7501 acgttagcca aagaagaaga taaattaaaa ctaattgctt tgttacgaac attctttaat
     7561 cgtttacatg ggtaccatat tcaatacaat gttgtttcca gagagacgct gattgacgct
     7621 cagaaacatc ctgaaaaaca cagagactta attgttcgtg ttgcaggata ctctgcattc
     7681 ttcaatgttc tttctaaggc aacccaagat gacattatag gacgtactga gcatactttg
     7741 taaaataaag aggttctttt tatggaattt atgcttgaca cattaaattt agatgagatt
     7801 aaaaagtggt ctgaaatttt gccgctagct ggggtaactt caaatcccac tattgcaaaa
     7861 agagagggtt ctattaattt ttttgaacga atcaaagatg taagagaatt gattggctct
     7921 acaccctcta ttcatgttca ggtgatttct caagattttg aaggcatctt aaaggatgct
     7981 cataaaattc gaagacaagc aggagatgat atatttatca aagtacctgt tactccagct
     8041 ggattacgtg caataaaggc gctaaaaaaa gagggctacc atatcactgc aacagctatt
     8101 tatacagtta ttcagggatt attagctatc gaagcaggag cggattacct agctccatat
     8161 tataatagaa tggaaaatct gaacattgat tcaaattctg tcattcgtca attagctctt
     8221 gctattgata gacagaactc tcctagtaag attttagctg catcctttaa aaatgtagca
     8281 caagtaaata atgctttagc tgcaggtgcg catgctgtta cagcaggagc agatgttttt
     8341 gaatcagctt tcgccatgcc atctatccaa aaggcggttg atgatttttc tgacgattgg
     8401 tttgttactc aaaatagtcg ttccatttag atagagagga aatacatatg agaatttttg
     8461 ctagtccttc tagatatatt cagggggaaa atgccttgtt tgaaaatgcc aaatcaattt
     8521 tggatttggg aaattacccg attctattat gcgatcagtt ggtttatgat attgttggaa
     8581 aacgatttga agattatcta cataggtatg gtttccatat tgttctggcg ctatttaatg
     8641 gtgaagcttc tgacaatgaa atcaatcgag ttgttgcctt ggctgagaaa gaaaattgtg
     8701 atagtattat cggtcttggt gggggaaaga cgattgatag cgcaaaagct attgcagatt
     8761 tgattgaaaa gcctgttatt attgctccaa caattgcatc gaccgacgca cctgtatctg
     8821 ctttatctgt tatttataca gatgaaggtg catttgatca ttatctattt tattctaaaa
     8881 atccagattt agttttggtt gatacaaaag ttatttcaca agcccctaag cgtttattag
     8941 cgtctggtat tgcagatggt ttagcaactt gggttgaggc gcgtgcggtt atgcaggcaa
     9001 atggaaaaac tatgttggga caacagcaaa cattggctgg agttgcaatt gcgaagaaat
     9061 gtgaagaaac gctgtttgca gatggtttac aggctatggc agcttgtgaa gctaaagtgg
     9121 tgacaccagc attagaaaat attgttgaag ctaatacttt attgagtggt ctaggttttg
     9181 aaagtggagg attagctgcg gcgcatgcaa ttcataatgg ttttactgca ttgacaggtg
     9241 acattcatca tttaacacat ggtgaaaaag tagcttatgg aactttagta caactattat
     9301 tggaaaatag acctaaagaa gaacttgata agtatattga gttttacaaa aaaattggta
     9361 tgccaacaac tctaaaagaa atgcatttgg atcaagttgg atatgatgat ttaataaaag
     9421 ttggtaaaca agcaactatg gagggtgaga caattcatca gatgccgttt aagatttcgc
     9481 cttcagatgt tgctcaagct attatcgctg tagatgccta tgtaaattca aaataaacaa
     9541 taaggactac tgttttccaa atggtagtct tttattgatc cctgtattga attctataga
     9601 agattgaaat a
//
LOCUS       DAVKAO010000064         9461 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKAO010000064 DAVKAO010000000
VERSION     DAVKAO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.064"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..84
                     /locus_tag="VWX16_001653"
     CDS             <1..84
                     /locus_tag="VWX16_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9158967.1"
                     /translation="LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            50..364
                     /locus_tag="VWX16_001654"
     CDS             50..364
                     /locus_tag="VWX16_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158968.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            1049..1210
                     /locus_tag="VWX16_001655"
     CDS             1049..1210
                     /locus_tag="VWX16_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158969.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1302..1786
                     /locus_tag="VWX16_001656"
                     /pseudo
     CDS             1302..1786
                     /locus_tag="VWX16_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861739.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1820..2980
                     /locus_tag="VWX16_001657"
     CDS             1820..2980
                     /locus_tag="VWX16_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9158970.1"
                     /translation="MFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQN
                     GKNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEA
                     IVSDEVWQAAQVKLKSQAKRYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKK
                     DGTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQE
                     KINMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLY
                     RMYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISA
                     LISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            3248..3814
                     /locus_tag="VWX16_001658"
     CDS             3248..3814
                     /locus_tag="VWX16_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158971.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4136..4324
                     /locus_tag="VWX16_001659"
     CDS             4136..4324
                     /locus_tag="VWX16_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9158972.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4386..5144
                     /locus_tag="VWX16_001660"
     CDS             4386..5144
                     /locus_tag="VWX16_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9158973.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     ISEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            5202..6056
                     /locus_tag="VWX16_001661"
     CDS             5202..6056
                     /locus_tag="VWX16_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9158974.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6168..7052
                     /locus_tag="VWX16_001662"
     CDS             6168..7052
                     /locus_tag="VWX16_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9158975.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7215..8954
                     /locus_tag="VWX16_001663"
     CDS             7215..8954
                     /locus_tag="VWX16_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEU9158976.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGEFMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            9229..>9461
                     /locus_tag="VWX16_001664"
     CDS             9229..>9461
                     /locus_tag="VWX16_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158977.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFK"
BASE COUNT         3365 a         1319 c         1992 g         2785 t
ORIGIN      
        1 ttgccagata tcaaaattaa attaccgata gacagtagag gtactccaga ttggaattac
       61 atggaaagat atagagatag ataaagagag agagggagag atagataaaa agtccacccc
      121 cattttttat ggagaattca aaaatgtaag gttaagcaaa gaagaatata aaaatctaaa
      181 agaaaaatta aactcacaca cagatataat gataaataaa ctatccagat acatggaaag
      241 cagtggtaag acctatcaaa accactatgt gacaatctta aaatggtatg aagaagataa
      301 agacaaacta agacagaaag gtttaaataa aaaaatgaat tatgatgtag gagaatcttt
      361 atagataaaa agaggtggaa aggcactatt aaagacttta agtctaaaaa ggtatcaaag
      421 tatgccagag ataaaaaaaa tataaatatg acactttaaa ggcgattaga aaattaaaga
      481 aatgctagaa gacttttaga tgacagttac caagagggtc taattttcac tgctatttat
      541 gcagtgggaa ttggaccttt ttatataagc aaattctatt tcaaaattgg ttaccaagaa
      601 agacttcttt tcactgttaa gtataagagg gtaatttata gaattttgtt acatcaagat
      661 aaatattttc tgatggtcaa attttaaaaa gattaataac tctcgataaa ttttgaacct
      721 taacaattga atagaccaag acaagacgtt aattattttt ggagcgtaat aacctatccg
      781 tcaagatctt tcggctgaat aattcggcaa aaaaagttct gctaagcaaa aagaaagtga
      841 gcgataagta agagtttgaa tatagggagg gataccctat ccgctatgaa gaaaatgagg
      901 gtaatgatac ttctaaggtt gaagccggct catccggaat agaggcggaa gtgagattcc
      961 tatgttgcta atccaaagca cttgtcaatg tcaaaaaact taattaaaca aataattatt
     1021 agatataaaa ctttagagga ggtatatatt ggacaatgac tggaatggat tggctgattt
     1081 aatagcaaat ctgattgcaa aatatgctgg tgctttagat ttagataatc tacccgatcc
     1141 aacgccagca aaaaatcaag agatgaaaaa tagttttgac atggcaaaaa cacaaattga
     1201 gacggactaa taaaagtgtt ataatatact tgaaatgata gtccaaactt aaattaaaaa
     1261 aattatatat gatatagaaa aattatattg aggttaatac tatgtcaaaa gaaaaaataa
     1321 aagtatacct ctatacacga gtatctacgt caatacagat agaggggtat tcgttagagg
     1381 cacaaaaatc acgaatgaaa gcttttgcta tttacaacga ttatgaaatt gttggagagt
     1441 atgaagatgc cgggaagtct ggcaagtcta ttgaaggtag aatacagttc aatcgaatga
     1501 tggaagatat aaaatctgga aaagatggag tatcttttgt tcttgtgttt aagttatcaa
     1561 gatttgcaag aaatgccgct gatgttcttt ctacacttca gataatgcag gattatggag
     1621 ttaatcttat ctgcgttgaa gatggcattg attcatctaa agatgctggg aaactaatga
     1681 tttctgtttt atcagctgtg gctgaaatag aaagagaaaa catccgtatt caaacaatgg
     1741 aaggtcgcat tcaaaggcaa gggaaggaaa atggaatggt ggctttgctc cgtatggata
     1801 taaacttgaa gacggcaagc tgtttataaa tgaggaagag gcagttgcaa taagaacgat
     1861 tttcgaccag tatgtaaata ctacgatagg agccaatggg atatctaaat acttagagaa
     1921 tcatggaatt agaaaaatcc caagacagaa tggtaagaat cctttgtttg atgcaggtct
     1981 tataagaaag atattaaaga atcctgtata taatgggaaa atagcatttg gaagaagaac
     2041 tttagaaaaa gttcatggta caagaaatga atataagcag gttgaacaag atgaatatct
     2101 aatatctgaa gggatacatg aagctatagt ttccgatgaa gtttggcaag ctgctcaggt
     2161 taagctaaaa tctcaagcaa agagatatga gcatgtgaat aaaggaaaag atacacgcac
     2221 acacttgctt tctggaattg taaaatgccc gatatgtgga gtgggaatgt ttgggaacaa
     2281 gtgtatcaag aaaaagaaag atggcacaaa gtataaagat ttttattact atggttgtaa
     2341 acataggcag atgataagag gtcataagtg tacattcagt aagcaaatta gagaagaatt
     2401 gttagatgat gctgttgcag aggtgattgt caagatagta agtaatccga aatttgcttc
     2461 tatgatgcaa gaaaaaatca acatgaaggt ggatacctct gaaatagaaa aagaaataga
     2521 taattaccaa aaggaattga gaaagagtca ttctacaaag tttaagctaa ttgaggaaat
     2581 agataattta gatgttgaag ataagcatta taagagaagg aaacaggatt tagatgatag
     2641 actatatcgt atgtatgaca aaatagatga attagaatca tcactaattg atgcgaaagc
     2701 aaagaaacag actattgaag ctgaaaagct tacaggagat aacatatata aggttttgat
     2761 ctattttgat aagctctata aagtcatgaa tgatgtagag cgtaggcagt taatttcagc
     2821 tttgatttct gaaattcaag tttatgaaga aaaacaatct aacggacaat ggctaaaatc
     2881 cattactttt aagctaccaa ttatagaaga aaatctcaac atagatttgg acaatgatga
     2941 acaagttgaa tgtgtaagtt tattagagaa acgtagttaa tcctcaaaag gttccccaaa
     3001 ccttttgagt cccactgacg catcacgctg aggcggtatc actgcttgta cgagttgaag
     3061 catcagcgaa gtaaaagtag atgttcagcc aatgcgacga ggtgagaccg aaggtcgata
     3121 aggtgtgttc tgggtatagg actcgtaaag taagtaggaa atatgatgag atgatacaga
     3181 gagcctgaat tggtcaggtg acgtgcttat ttacattagc ctgataatga aaatggagag
     3241 attattgatg gttatattgg aattgtacca aaatgattat tctaaagatt tagtagcatt
     3301 tgattccata gaagatggga aagcctttgt agctcaaatc ccgggataca ctctggaaac
     3361 agaagatggt tttgaagtgg agtattttaa tcctaaaaac ataccggatt atatggaaat
     3421 tatttttaac ggaaatattg ttcctttatc taaatttatg ttcgatcctg aggaaaatgt
     3481 aaacattatt tggaaagaga tttcaaattt atccctcaaa aatgatagag tgatcgaagg
     3541 atattcaaaa attgatgcat atgtcgttaa caaccatgaa gttaaagcct atgtcgaaac
     3601 aagagaaacg aactatcgta aagcaaaaga cttcttagaa agccgcggat atgaaatcga
     3661 cagaagcttt ttcggaagtg aagacggtga agcgattctt tacagaaaaa aagggatcga
     3721 agtttggcat ttcttgtgtc acttagaccc aatgtttgta gagattgaag atgtagaagg
     3781 atatgttaaa gaagaagttg gagagattca atagtcaacc aaagttcctt gtgagtatat
     3841 acctttgcta gtaaaagagt caaaacgtta taacgaaggg gggtaagaaa acccttgagt
     3901 gtaagtcttt ttcttgttta gagggaaagt attagagtag ccgctcataa tttatactag
     3961 atttgataac tgatttgaga cagattttat ttctataaat attgtgaaaa ttttttaata
     4021 ccaaagattt aaagttactg tgttaaaaat cctctccact tttaaactaa tgtattataa
     4081 aagccagttg acacaagtgt tacacctgtg atagaattaa cttgaggtga tgaaaatggc
     4141 aaccgataaa aatagaataa tgattagctt ggatgataaa aatctagaga aacttgaaaa
     4201 tttagtagaa gatgctagag atagaagggg aatgcgacta acaaagtccc aggttattga
     4261 attactccta aataccgttg attatttcga tgatattatg ggggctattt actcaaaaaa
     4321 ataagagcaa aattgctatt attttttaca aattggtgta acactagtga tataggagtg
     4381 atgaaatggt tgtttatatt agacaaagta aattaccaag tgaggtttca attaataaat
     4441 acaatgctca agtaggtgcc tacttacaag gagaagaagt tattttatat caatcttttt
     4501 ctgaaataaa agagttaata agtgaagata tagttgtaga ttatataatg gagactagag
     4561 cattactaaa aatgatgggc ttaaacgttc cggtttatga ctatcctatt gagcttaaag
     4621 agttttatgg tcgaaagatt tatgctggta ttttaggaga gattgtgaat atacctgata
     4681 attggggaaa atttattaag cctaaagctg gttcaaaagt ctttactgga agagttgtta
     4741 atggaaccca tgatttaata ggtattggtc tacctttcga ctatcctata tggattagtg
     4801 aggttgtaga attcatagct gaatggcgtt gttttgtgtt agatggtcgc gtattagatg
     4861 ttcgacccta tacaggtgat tatcatgcac aatttgatgc aagtgtaatt gatgaagcga
     4921 tatcatgttg gaaagatgcg ccaatagctt atggactaga tatcggtgtt actcgcgatg
     4981 gcagaacact tgttgttgaa gtaaatgatg gttatgcatt gggaaattat ggcctatctc
     5041 ctttaaaatc aatcaatttt catagagcta ggtggaaaga aatggtaaaa ccctattttg
     5101 aaaaaaatga aatttttaaa atccaacagg atgttatttt ctaaaataaa tatggtagat
     5161 aaccaatata aaaagcatca aaatgaacaa ggaggaatct gatgcttgga gcaattattg
     5221 gagatattgt cggttctgtt tacgaatgga acaatatcaa aacaaaggac tttcctttat
     5281 ttcggaagga ctgctttttt acagatgaca cggttatgac ctgtgctgtt gcagaagcaa
     5341 ttatgaatgg tggacaaaaa gacgatttca ttgacgcgat gaagaaatat ggcagaatgt
     5401 atccgaatgc tgattacggt gctcggttta atgcatggct aaacagcgat aaccgtgagc
     5461 cttataatag ctttgggaat ggatcagcta tgcgtatttc tccatgtgct tggatcatgg
     5521 actgtggttt ttatgcgaaa actggtatgt ggccatcatc tagaggactt acgagtcttt
     5581 ctgcagaggt aactcataat catccagaag gtgtcaaagg cgctatggct acagctgatg
     5641 ctatctttct atgtcgtttt tactttggtg gttattgtag agagtacgag caatcaatta
     5701 acgacaatcc tacagagtgt aaaagacgaa ttaaggatta tatagaaaaa gaatacgatt
     5761 acaatctatc tcaaacttta gatgaaatcc gtcctaacta tcgttttaac gaaacatgtc
     5821 aggaaactgt ccctcaagct attatcgcct ttcttgaaag tagagacttc gaagatgcga
     5881 taagaaatgc catctcactt ggtggcgaca gtgatactct cgcagcaatc acttgtaaca
     5941 tagcagaggc agcttacggt attcctgatt ggatcaagga taaggcctat tcttacttgg
     6001 atgaaccctt aaaggatgta gttaggcgat gggaaaatag aataaaagcg tattaatctc
     6061 cagtacgtca gatttctctt ttctgtattt tcattgtata ataggattac atttacttgc
     6121 atacgacgaa atgtaaacat ttctgtttaa aattaaggat aaaatcaatg aaaaaagcaa
     6181 tggtaattat caaccctact tctggtggcg agaaggcttt ggattacaaa gaaaagctgg
     6241 agaataaagc aaaagaatac tttgaatatg ttgaaaccaa aattaccgaa aaagcgctgg
     6301 atgcaacaca ttttgctgaa gaagcttctc gtgagcagta tgatgcagtg gttgtgtttg
     6361 gtggagatgg aactgtcaat gaagtcattt caggtattga tgagagagac tacattccta
     6421 agttagggat tatcccaggc ggtacgggta acctcattac aaaacttttg gaaatcaatc
     6481 aagacatcga tggcgcaatt gacgaactgg attttgattt aaccaataag attgatatcg
     6541 gtaaagcaaa tgacaactat tttggttata tctttagtat cggttctctg cctgaggcga
     6601 ttcacaatgt tgaaatagag gacaaaacaa aattcggtat tttaacctat gctgtaaata
     6661 ccatgaagtc tgtcatgaca gatcaggtct ttaacattaa ggttgagaca gaaaatggaa
     6721 attatgttgg tgaagctagc catgttttgg tccttttgac aaattacttc gctgataaga
     6781 aaatctttga agaaaacaag gacggctatg ccaacatttt gattctgaaa gatgcctcta
     6841 tattctccaa attatccgtc attcctgatt tattaaaagg ggatgttgtc gcaaatgata
     6901 atatcgagta tatcaaagcg cgtaatatta aaatctcttc agatagtgaa ttggagtcag
     6961 atgttgacgg agataaatca gataacctac ctgtagaaat caaagtccta gctcagcgag
     7021 tagaagtatt ttcaaaaccg aaagaggatt agtatataga gaaagccttt tttaaggctt
     7081 tttgtatact ttaaaagata gttcctttaa caacggacat tccttgcaaa tagtttacaa
     7141 aaatagtata ctggattcat tgagtttgaa aacgtttgcg taaaatttga atgaatactt
     7201 taggagacaa attgatggaa ttgagtgcta tttaccatag gcctgagtcg gagtatgcct
     7261 atctttataa ggataagaaa ctccatattc gaattcgaac taagaaaggg gacattgaaa
     7321 gcatcaactt gcactatggg gaccctttta tctttatgga ggagttttat caggatacaa
     7381 aagaaatggt caagataact tctggtacct tatttgacca ttggcaggtt gaagtgtcag
     7441 ttgactttgc acgtatccag tatctctttg agctcagaga tacagaaggt caaaatattt
     7501 tgtatggcga taaagggtgt gtggaaaatt ctctagaaaa tcttcatgca atcgggaatg
     7561 gatttaagtt gccttatctt catgagattg atgcctgcaa ggttcctgac tgggtttcaa
     7621 atacggtatg gtatcagata tttcctgaaa ggtttgccaa tggcaatgct ctattaaacc
     7681 cagaagggac tttagactgg gattcatctg tcacacctaa gagcgatgat ttctttggtg
     7741 gtgatttaca ggggattatt gatcatatgg attacttgca agacttgggt attactggac
     7801 tatatctttg tcccatcttt gaatctacaa gcaatcacaa gtacaatacg acagattact
     7861 ttgaaattga ccgtcatttt ggagacaagg agacctttcg ggaactggtg gatcaagcgc
     7921 atcatcgtgg catgaaagtc atgctggatg cggtatttaa tcatattggt tcgcaatctc
     7981 ttcaatggaa aaatgtcgtc aaaaatggtg aacagtctgc ttataaggat tggttccata
     8041 ttcaacaatt cccagtgaca actgaaaagc tagttaataa gagagactta ccctatcatg
     8101 tttttggttt cgaggactat atgcctaagc taaatacagc caatccagag gtcaagaatt
     8161 atcttttaaa ggttgcgact tattggattg aagagtttaa tatcgatgct tggcgtttgg
     8221 atgtggctaa tgagattgac catcagttct ggaaggattt tcgtaaggca gttttagcta
     8281 aaaatcctga tctttatatc ctaggagaag tctggcatac atctcagcct tggctaaatg
     8341 gagatgagtt ccatgccgtc atgaattatc ctttatctga tagtatcaag gactatttct
     8401 tacgaggaat taagaagaca gaccagttca tcgatgaaat caatggagag tttatgtatt
     8461 acaagcagca gatttcagag gtcatgttta atctcttgga ttcacatgat acagagcgaa
     8521 tcctgtggac ggccaatgaa gatgttcaac tggttaaatc agccttagcc tttctctttt
     8581 tacaaaaagg aacaccgtgc atttattacg gaaccgagct agccttgact ggaggaccag
     8641 atccagattg tcgtcgttgt atgccttggg aacgtgtatc aagtgacaat gatatgctga
     8701 actttatgaa gaggctgatt aaaattcgga aatacgcgtc agtaatcatt tcgcatggca
     8761 agtatagcct tcaagaaatc aaatctgatc tagtagctct ggaatggaaa tacgaaggac
     8821 ggatcctcaa agcaatattc aaccaatcaa cagaagatta tcttttagag aaagaagcag
     8881 tagcactagc aagcaattgc caagaattgg agaatcagct tgtcatctct ccagatggat
     8941 ttgtgatttt ctaaaaacta gttgatgaag attatggtac atttcatatc ttatatagta
     9001 taataaggct agttactaaa cttgtaaagg agaacttaaa tgaattgtag aggacatgaa
     9061 acaagacaaa gaattgttag agattttgaa gtttagccta aagcacatat taagctgtta
     9121 gcaaatcaac aaaaacatag tgatgcagga gcaactattg aagatgaata ttatgtattt
     9181 atcgctgaga gtaaaattga tggcaagaag gaagttattc agtgttgcat gggtgcggca
     9241 agggattttt tagaactaat taatcacaaa gggctacctc tttttaatcc gcttgtaggt
     9301 gattctcatg taaataatag acaagaatat gacaatacag ggagtggaaa tttacaacct
     9361 gaaaagtgga atgaaactgc aaagcagctt tataatgtta taatgtggtt gattatttta
     9421 tggaatgcta agccggatac acctttattt aattttaaag a
//
LOCUS       DAVKAO010000065         9456 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKAO010000065 DAVKAO010000000
VERSION     DAVKAO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.065"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..5756
                     /locus_tag="VWX16_001665"
     CDS             <1..5756
                     /locus_tag="VWX16_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9158978.1"
                     /translation="KYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQETA
                     GVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSKNE
                     RQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLLNY
                     NTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDYNV
                     TPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTKLINPRKEEKQSSDSQEQLAE
                     HKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEIQE
                     NPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTKKT
                     QVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALSEAVVTDKGET
                     EVQPESSDTVVSDKGEPKQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVKPT
                     EETPVNPNEGTTEGTSIQGAENPVQPAEESTTNSEKVSPDTSSENTGEVSNKPSDSKP
                     PVEESNQPENSGNTTSENGQTEPEPSNGNSTENVSTKSNTSNSNGNEEIKQENELDPD
                     KKVEDPEKTLELRNVSDLELYSLSNGTYKQHISLEQVPSNPNSYFVKVKSSSFKDVYL
                     PVASISEERKNDKILYKITAKVEKLQQEIESRYKDNFTFYLAKKGTEETTNFTSFSNL
                     VKAINQNLSGTYHLGASLNANEVELSTDDKSYIKGTFTGQLIGEKDGKHYAIYNLKKP
                     LFESLRGATIEKLSLKNVSISGKDDIGSLANEAQNNTKIKQVHVDGVLAGERGIGGLL
                     AKADQSSITESNFKGRIVNTYETTASYNIGGLVGHLTGSKASLTKSKATVVISSNTNS
                     SDQTVGGIAGLVDKDAHIQNSYSEGDINNSQRFGKVAGIAGNLWDRESNSENHAGRLT
                     NVLSDVNVTNGNAISGYHYNGMKITDAFSNKANKVFNVTLEKDEVVSKESFEERGTML
                     DASQIASKKAEINLLTPPIVKPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYN
                     KATIVKYGNLVKENSILYQKELLSAVMMKDDQVITDIISNKQTANKLLLHYKDHSSEK
                     FDLRYQADFAKLAEYSLGDTGLLYTPNQFLYDQDSIINQVLPELQQVAYDSEAIRKTL
                     GISPEVKQTELYMEDQFTKTKQDLANSLKKLLSADAGLAGDNPVTRGYLVDKIKNNKE
                     ALLLGLTYLERWYNFSYGQVNVKNLVMYHLDFFGKGNTSPLDTLIELGKSGFNNLLAK
                     NNVDTYAISLASHHGTTDLFSTLENYRKVFLPDKTNNDWFKSQTKAYIVEEKSNIEEV
                     KTKQGLVGTKYSIGVYDRITSDSWKYRNMVLPLLTLPERSVFVISTISSLGFGAYDRY
                     RNKEHQANGDLNSFVEKSAHETAERQRDHYDYWYRILDEKGREKLYRNILLYDAYKFG
                     TDHTEGKATEVANFDNPNPAMKHFFGPVGNKVGHNGHGAYATGDAVYYMGYRMLDKDG
                     AITYTHEMTHDSDQDIYLGGYGRRSGLGPEFFAKGLLQAPDQPSDATITINSILKHKT
                     SDSTEGQRLQVLDPTTRFNDAADLQNYVHNMFDVVYMLEYLEGQSIVKQLDAYQKMTA
                     LRKIENKYVKDPADGNDVYATNVVKNLTEDEAKKLTSFDSLIDNNILSAREYKAGTYE
                     RNGYFTIKLFAPIFSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDA
                     KQQGQTINLYGKERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFK
                     DPTKRWPSYGTKTINNVDELQKLMDEAVLQDATGTRWSNYNPEIDSAVHKLKRAIFKA
                     YLAQTNDFRSSIFENKK"
     gene            5808..5984
                     /locus_tag="VWX16_001666"
     CDS             5808..5984
                     /locus_tag="VWX16_001666"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9158979.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            6203..7054
                     /gene="ylqF"
                     /locus_tag="VWX16_001667"
     CDS             6203..7054
                     /gene="ylqF"
                     /locus_tag="VWX16_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9158980.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQEIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            7041..7820
                     /locus_tag="VWX16_001668"
     CDS             7041..7820
                     /locus_tag="VWX16_001668"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9158981.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            7836..9386
                     /locus_tag="VWX16_001669"
     CDS             7836..9386
                     /locus_tag="VWX16_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9158982.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHMIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3215 a         1706 c         1931 g         2604 t
ORIGIN      
        1 aaaagtattt tggtgaaaaa caagagcgtt tttcatttag aaaattatca gtaggacttg
       61 tatctgcaac gatttcaagt ttatttttta tgtctgtatt agctagttca tctgtggatg
      121 ctcaagaaac tgcgggagtt cactataaat atgtggcaga ttcagagcta tcatcagaag
      181 aaaagaagca gcttgtctat gatattccga catacgtgga gaatgatgat gaaacttatt
      241 atcttgttta taagttaaat tctcaaaatc aactggcgga attaccaaat actggaagca
      301 agaatgagag gcaagcccta gttgctggtg ctagcttagc tgctctggga attttaattt
      361 ttgctgtttc caagaaaaag gttaagaata aaacggtatt acatttagta ttggttgcgg
      421 gaataggaaa tggtgtctta gtttcagtcc atgctttaga aaatcatctt ttgctaaatt
      481 acaatacgga ctatgaattg acctctggag aaaaattacc tcttcctaaa gagatttcag
      541 gttacactta tattggatat atcaaagagg gaaaaacgac ttctgatttt gaagtaagta
      601 atcaagaaaa atcagcagcc actcctacaa aacaacaaaa ggtggattat aatgttacac
      661 caaattttgt agaccatcca tcaacagtac aagctattca ggaacaaaca cctgtttctt
      721 caactaagcc gacagaagtt caagtagttg aaaaaccttt ctctactaaa ttaatcaatc
      781 caagaaaaga agagaaacaa tcttcagatt ctcaagaaca attagccgaa cataagaatc
      841 tagaaacgaa gaaagaggag aagatttctc caaaagaaaa gactggggta aatacattaa
      901 atccacagga tgaagtttta tcaggtcaat tgaacaaacc tgaactctta tatcgtgagg
      961 aaactataga gacaaaaata gattttcaag aagaaattca agaaaatcct gatttagctg
     1021 aaggaactgt aagagtaaaa caagaaggta aattaggtaa gaaagttgaa atcgtcagaa
     1081 tattctctgt aaacaaggaa gaagtttcgc gagaaattgt ttcaacttca acgactgcgc
     1141 ctagtccaag aatagtcgaa aaaggtacta aaaaaactca agttataaag gaacaacctg
     1201 agactggtgt agaacataag gacgtacagt ctggagctat tgttgaaccc gcaattcagc
     1261 ctgagttgcc cgaagcagta gtaagtgata aaggcgtacc agaagttcaa ccagcgttat
     1321 cggaagcagt cgtaaccgac aaaggtgaga ctgaggttca accagagtcg tcagatactg
     1381 tggtaagtga taaaggtgaa cctaaacagg tagcaccgct tccagaatat aagggtaata
     1441 ttgagcaagt aaaacctgaa actccggttg agaagaccaa agaacaaggt ccagaaaaaa
     1501 ctgaagaagt tccagtaaaa ccaacagaag aaacaccagt aaatccaaat gaaggtacta
     1561 cagaaggaac ctcaattcaa ggagcagaaa atccagttca acctgcagaa gaatcaacaa
     1621 cgaattcaga gaaagtatca ccagatacat ctagcgaaaa tactggggaa gtatccaata
     1681 aacctagtga ttcaaaacca ccagttgaag aatcaaatca accagaaaat tcaggtaata
     1741 caacatcaga gaatggacaa acagaaccag aaccatcaaa cggaaattca actgagaatg
     1801 tttcaaccaa atcaaacaca tccaattcaa atggaaacga agaaattaaa caagaaaatg
     1861 aactagaccc cgataaaaag gtagaagatc cagagaaaac acttgaatta agaaatgttt
     1921 ccgacctaga gttatacagt ttgtcaaatg gtacttataa acaacacatt tcgttagaac
     1981 aagttccaag caatccaaat agctactttg ttaaagtgaa atcttcttca ttcaaagatg
     2041 tatacctacc agtagcatca atatcagagg aaagaaaaaa tgataaaatc ctttataaaa
     2101 tcacagcaaa agtagagaag cttcagcagg agatagaaag cagatataaa gataatttta
     2161 ccttctatct agctaagaag ggaacagaag aaacaacaaa ctttacttcc tttagtaatc
     2221 tggtcaaagc tataaaccaa aatctctctg gaacctatca tttaggagct agcctgaatg
     2281 ctaacgaagt ggagctcagt actgatgata aatcctatat caagggaacc tttactggtc
     2341 agttgattgg tgaaaaagat ggtaagcatt atgctattta caacttgaaa aagcctcttt
     2401 ttgaaagctt gagaggggcc acaatagaaa agttgagtct gaaaaatgtc tctatttcag
     2461 ggaaagatga tatcggttca ctggcaaatg aagctcagaa taacacaaaa attaagcaag
     2521 ttcatgtgga tggtgtactt gccggtgaac gtggtatcgg tggtttgttg gctaaggcag
     2581 atcaatcaag catcacagag agtaatttca agggaagaat tgtcaatacc tatgaaacaa
     2641 ccgcttccta taatatcggt ggcctggtcg gtcatttgac aggaagcaaa gcttcactga
     2701 ctaagtcaaa agcgacagtg gtcatttcat caaatacaaa tagttctgac cagaccgttg
     2761 gaggtatcgc tggtcttgta gacaaagatg cgcatattca gaatagctat tctgaagggg
     2821 atattaataa tagtcaacgt tttggaaaag ttgctggtat agcaggtaac ttatgggatc
     2881 gtgaatcaaa tagtgaaaat catgctggaa gattgactaa tgtgcttagt gatgtcaatg
     2941 taactaatgg aaatgccatt agtggttacc attataatgg aatgaaaatt actgatgcat
     3001 ttagtaataa agcgaataaa gttttcaatg tcactttaga gaaggatgag gtcgtcagca
     3061 aagaatcctt tgaggaaaga ggaacaatgc tagatgcttc tcaaattgca agcaaaaaag
     3121 cagaaatcaa tcttctcact ccaccaatag tgaaacccct ctcaacaagc ggtaagaaag
     3181 acagtgattt ttctaagata gcccattatc aagccaaccg tgctttggtt tataagaaca
     3241 ttgaaaaatt gttacctttc tataacaagg cgaccatcgt gaaatacgga aacctggtta
     3301 aggagaacag tatcttatat caaaaagaac tcttgtctgc agttatgatg aaggatgacc
     3361 aagtaatcac agatattatt tccaacaaac agactgcaaa caaactcttg cttcactaca
     3421 aggaccattc atctgaaaag tttgatctca ggtaccaggc tgattttgct aagttagcag
     3481 aatatagtct aggagatact ggtcttctct atacgccaaa ccaattcttg tatgaccaag
     3541 actctatcat caatcaagtt ttacctgaat tacagcaggt tgcctacgat tcagaagcta
     3601 tcagaaagac actcggaatt tctccagagg tcaagcaaac tgagctctat atggaagacc
     3661 agtttaccaa aacaaagcaa gatttggcaa acagtttgaa aaaactcttg tctgcagatg
     3721 ctggacttgc tggtgacaac ccagttacca gaggctatct tgtagataaa atcaagaaca
     3781 ataaggaagc cttactactc ggtttaactt atttagaacg ttggtataac tttagctatg
     3841 gtcaagtgaa tgtcaaaaac ctagttatgt atcatctgga cttctttggt aaaggaaata
     3901 cctcaccatt agatactctg attgagttag gtaaatctgg ctttaacaat cttcttgcta
     3961 aaaacaatgt cgatacttat gctatcagtc ttgccagcca tcatggaacg acagatttgt
     4021 ttagcacgtt ggaaaattac cgaaaagtct ttctaccaga caaaaccaat aatgactggt
     4081 ttaaatcaca gactaaggct tacattgtcg aagaaaaatc caatatcgaa gaggtgaaaa
     4141 cgaagcaagg actagttggt actaagtatt ctattggtgt ctatgatcga atcactagcg
     4201 acagctggaa ataccgtaat atggtactac ctctacttac cttgccagaa agatccgtat
     4261 ttgtcatttc aaccatatct agtctaggtt ttggtgctta tgatcgctat cgtaataaag
     4321 aacatcaagc aaatggtgat cttaatagct ttgttgaaaa gagtgctcac gaaacagcag
     4381 agcgtcaacg agatcactat gattattggt atcgtattct agatgaaaaa gggcgtgaaa
     4441 agctttatcg aaatatcttg ctttatgatg cctataaatt tggaactgat catactgaag
     4501 gaaaggctac agaagtagca aattttgata atccaaatcc tgcaatgaaa catttctttg
     4561 gacctgttgg aaataaggtt ggacataatg gacatggtgc ttatgctaca ggggatgccg
     4621 tttattatat gggttatcgt atgttggaca aggacggcgc cattacttat acgcatgaga
     4681 tgacacatga ctcagatcag gacatttatc ttggaggtta tggaagaaga agtggacttg
     4741 gacctgagtt ctttgctaaa ggcttactac aagcaccaga ccaaccaagt gatgcaacca
     4801 ttaccatcaa ctctatcttg aaacataaaa catcagatag tacagaaggt cagcgattgc
     4861 aagtacttga tccaactaca agatttaatg acgcagcaga tcttcagaac tacgtccaca
     4921 atatgtttga tgtcgtttac atgttggaat atctcgaagg gcaatctatc gtgaaacagt
     4981 tagatgctta tcagaaaatg acggccctga gaaaaatcga gaataaatac gtgaaagatc
     5041 ctgcagatgg aaatgatgtt tatgctacta atgttgtgaa aaatttgact gaagatgaag
     5101 ctaaaaaatt gacgagcttt gatagtttga ttgacaataa cattctttcg gctcgtgagt
     5161 ataaggctgg cacttatgag agaaatggct actttactat aaaactcttt gcaccaatct
     5221 tttcagcatt gagcagtgag aaaggaactc caggcgacct tatgggacgt cgtatagcct
     5281 atgaactact agctgctaaa ggctttaaag atggtatggt accatatatc tcaaatcaat
     5341 acgaagaaga tgctaaacaa caagggcaaa ctatcaatct ttatggtaaa gaacggggct
     5401 tggtgaccga tgagcttgtt ttgaaaaagg tatttgatgg taagtataaa acttgggcag
     5461 aatttaagac agctatgtac caagaacggg tggatcagtt tggaaacttg aagcaggtga
     5521 cctttaaaga tccgacaaaa cgatggccaa gctatggcac aaagactatc aataatgtgg
     5581 atgaattgca gaaactaatg gatgaagctg ttttgcagga tgctacaggt acacgttgga
     5641 gtaattataa tccagaaata gacagtgcag ttcataagtt gaagagagca atctttaaag
     5701 cctatcttgc tcaaaccaat gattttagaa gttcaatttt tgagaataaa aaatagtgtt
     5761 tactattagg aaataaagtt taaaaaggtg atgaagaaca aaccaagatt caagcaggaa
     5821 ttcctactga taatgaagta agttatgctc ttatttatca gcaggaaact cttcctgcaa
     5881 caggttcatc aacttctgcg cttacagctt taggcctatt agctgttggt agtttagttc
     5941 ttttggttca taatatgacg ggaacagttt tttgctccct ctgaaaagtc atcatttgat
     6001 ggcttttttc tatatagggt aaaagatagg gtaaaaggct atcatcggac aaaataaaga
     6061 aggcatgata taatataaag tagatttcta tgtcataaaa caagaactgt ttggacatca
     6121 tccatttgaa gactctctat gttcaaacta tagtaaaata aaatagggga tctaaatcct
     6181 tgctacgaaa ggaaaaaact caatggctac tattcaatgg tttcctggtc acatgtctaa
     6241 agcgcgtcga caggtgcagg aaaatttaaa atttgttgat tttgtgacga ttttagtaga
     6301 tgcacgcttg cctctatcta gtcaaaatcc tatgttgacc aagattgttg gtgataaacc
     6361 aaaactcttg attttaaaca aggccgactt ggctgatcca gcaatgacca aggaatggcg
     6421 tcagtatttt gaatcacaag aaatccagac gctagctatc aactccaaag agcaagtgac
     6481 tgtaaaagtt gtaacagatg cggccaagaa gctcatggct gataagattg ctcgccagaa
     6541 agaacgtggg attcagattg aaaccttgcg taccatgatt atcgggattc caaacgctgg
     6601 taaatccact ctgatgaacc gtttggctgg taaaaagatt gctgttgttg gaaacaagcc
     6661 aggggtcaca aaaggtcaac aatggcttaa aaccaataaa gatctggaaa tcttggatac
     6721 accggggatt ctctggccta agtttgagga tgaaactgtt gcacttaagt tggcattgac
     6781 tggagctatc aaggatcagt tgcttcctat ggatgaggtt accatttttg gtatcaatta
     6841 tttcaaagaa cattatccag aaaagctggc tgaacgcttc aaacaaatga aaattgaaga
     6901 agaagcgcct gtgattatta tggatatgac ccgcgccctc ggtttccgtg atgactatga
     6961 ccgtttttac agtctcttcg tgaaggaagt ccgtgatggc aaactcggta actatacctt
     7021 agatacattg gaagacctcg atggcaacga ttaaagaaat caaagaactc cttgtgacag
     7081 tcaaggagtt agaaagccct atttttttag agcttgaaaa ggataatcgc tcaggagttc
     7141 aaaaggaaat cagcaagcgt aaaagagcca ttcaagctga attagatgaa aatttgcgct
     7201 tggaatccat gctttcttat gaaaaagaac tttataagca aggattgacc ttaattgtag
     7261 gtattgatga ggttggtcgt ggtcctcttg ctggtcctgt agtcgctgcg gccgtcattt
     7321 tacctaaaaa ttgtaagatt aaaggtctca acgacagcaa gaaaattcct aaaaagaaac
     7381 atctggagat tttccaagcc gttcaagacc aagccttgtc gattggaatt ggtatcatag
     7441 ataatcaggt catcgaccaa gtcaacatct atgaagcaac caaactagcc atgcaagaag
     7501 caatctccca gctcagccct caaccagagc accttttgat tgatgccatg aaactggact
     7561 tgcccatttc acaaacctcc attatcaaag gagatgccaa ctccctctct atcgcagcag
     7621 catctatagt agccaaggta acacgtgatg aattgatgaa agaatacgat cagcagttcc
     7681 ctggctatga tttcgctact aatgcaggat atggcacagc taaacatctg gaaggcctca
     7741 caaaactagg agttacccca attcaccgaa ccagctttga acccgttaaa tcactggttt
     7801 taggtaaaaa agaaagttaa ttgaaaggaa ataccatgga ggaacagtcg gaaatagtcc
     7861 gttctaagaa agaattcgcc tttgcatcca gcactatact atcccaagtt ggtcgaggaa
     7921 tcattgtcgg cctcatcgtt ggaattatcg tcggatcctt tcgtttctta attgaaaagg
     7981 gcttccacat gatacaagga gtttatcaag atcaagggta cttagtgcgc aatctttttg
     8041 tactggtttt gttttatata ctcatctgtt ggctcagtgc caaactaaca cggtcagaaa
     8101 aagatattaa aggctcagga attcctcaag tcgaagccga actgaaaggc ctcatgtccc
     8161 tcaactggtg gggcattctt tggaaaaaat atgtgctagg tattcttgct attgccagtg
     8221 gactcatgct gggtcgagag ggacccagta ttcaacttgg agcagttggt ggtaaaggaa
     8281 ttgccaagtg gctcaaatcc agtccagtag aggaacgttc cttgattgcc agtggagctg
     8341 cagcaggttt agccgcagcc tttaatgctc ctattgcagc acttctcttt gttgtagaag
     8401 aagtctatca ccatttttcg cgctttttct gggtctcaac tctagcagcc agcatcgtag
     8461 caaactttgt gtctctactc atgttcggtt tgacaccagt attggatatg ccagataaca
     8521 ttcctcccat gaccctagat cagtattgga tatatctcgt catgggaatt ttccttggat
     8581 tttcaggttt tctctatgag aaagctgtat taaacgttgg aagagtttat gacttgattg
     8641 gtcaaaaaat ccatttggat agggcttatt atcccatctt ggcttttatc cttatcatac
     8701 cagtcggaat cttcttacct caaatcattg gtggcggaaa tcagcttgtc ctttctttaa
     8761 ctgaacaaaa ttttagtttc caagttttat tagcttactt tttaatccgc tttatttgga
     8821 gtatgattag ctatggaagt ggactgccag gaggaatttt cctccccatt ttagctcttg
     8881 gttctttgct tggtgcctta gttggtgtta tctgtgtcaa tcttggactt gtcagtcaag
     8941 agcaattccc tatatttgtc attctaggaa tgagtggcta ttttggagcc atatcaaaag
     9001 ctcccttaac cgctatgatc ctcgtaactg agatggtagg agatattcgc aaccttatgc
     9061 cacttggtct tgtcactctt gtttcttata ttatcatgga tttgctcaaa ggtacgccag
     9121 tctatgaagc catgctggaa aaaatgcttc cagaagaagt atctagcgaa ggagaagtta
     9181 cacttatcga aataccagtt tctgataaaa ttgctgggaa acaagttcat gaactcaact
     9241 taccacacaa cgtcctcatc acaactcaag tccataatgg caagagccaa acagttaacg
     9301 gctcaaccag aatgtatctg ggtgatatga ttcacctggt tattccaaaa agtgaaattg
     9361 gaaaagtcaa agatttgttg ttgtagtatg atcatttaca taatttatgt tatgtaaatg
     9421 atcggtttga tttatttaga aaaccgattc tcagaa
//
LOCUS       DAVKAO010000066         9401 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKAO010000066 DAVKAO010000000
VERSION     DAVKAO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.066"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            479..1522
                     /locus_tag="VWX16_001670"
     CDS             479..1522
                     /locus_tag="VWX16_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9158983.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1689..3776
                     /gene="topA"
                     /locus_tag="VWX16_001671"
     CDS             1689..3776
                     /gene="topA"
                     /locus_tag="VWX16_001671"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9158984.1"
                     /translation="MATATKKTKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISSVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLTIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3997..4356
                     /locus_tag="VWX16_001672"
     CDS             3997..4356
                     /locus_tag="VWX16_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9158985.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4449..5081
                     /locus_tag="VWX16_001673"
     CDS             4449..5081
                     /locus_tag="VWX16_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9158986.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            5125..5781
                     /locus_tag="VWX16_001674"
     CDS             5125..5781
                     /locus_tag="VWX16_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9158987.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5800..6432
                     /locus_tag="VWX16_001675"
     CDS             5800..6432
                     /locus_tag="VWX16_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9158988.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEETINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6460..6894
                     /locus_tag="VWX16_001676"
     CDS             6460..6894
                     /locus_tag="VWX16_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9158989.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6906..7942
                     /gene="leuB"
                     /locus_tag="VWX16_001677"
                     /pseudo
     CDS             6906..7942
                     /gene="leuB"
                     /locus_tag="VWX16_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            7939..8208
                     /locus_tag="VWX16_001678"
     CDS             7939..8208
                     /locus_tag="VWX16_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9158990.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYYIWR"
     gene            8212..8571
                     /gene="leuD"
                     /locus_tag="VWX16_001679"
     CDS             8212..8571
                     /gene="leuD"
                     /locus_tag="VWX16_001679"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9158991.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8733..9301
                     /locus_tag="VWX16_001680"
                     /pseudo
     CDS             8733..9301
                     /locus_tag="VWX16_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2925 a         1651 c         2088 g         2737 t
ORIGIN      
        1 actgttatag attcaataca ctataattta gtgtaattga gaaaggagaa atgattgtga
       61 ttgatgttgg ctaggttatt ttcaatgatt cctaccgtct caaatcttgt cagtaaggaa
      121 aaataaattc ttcaaaagta gagattacaa ggcttgttta agaaagaatt caaagacctt
      181 gacaaataaa aataaaatgg ttattataag aaatggtcta aaatagatga tgatactttt
      241 cgaaaatctt tttacgtcag ctcagctttg ccttgctgtg ttttgagcaa gctacggtta
      301 gcttccgagt ttgattttca tttactagaa atgaaactga tgagagatat cagtagacat
      361 ttgagtcagg atattatgga aaatgataaa aagagctcgt gagattggca tatcagacta
      421 ctaaagtatt gagtttgtta ggattttagc gactagttag ctgggaaagg aagatattgt
      481 gacaaataat aaactgtatt cgttgataga atttagaaat aaaatatatg aagaattaga
      541 actttccaga agtgatttag cgattttact atgtgccatg cttatcgcct ctatcggatt
      601 aaatatggat tcgactcccg tgattattgg agccatgtta atctctcctt tgatgacacc
      661 tattctggga gtggggctct ctctagctat atttgatttt aaattgttaa gaaaatcttt
      721 taaaatatta gctattcaaa ttcttgccag tttaatagct tcaacacttt atttttatct
      781 ttctcccatt tcgtatgcta gttcggaaat tgttgctaga acctctccga ctatttggga
      841 tgttctcatt gcttttgtag gagggatagc aggtattatt ggtgctagga aaaaagagac
      901 caataatatt gttcctggtg ttgctattgc aaccgccttg atgcctcctc tttgtacagt
      961 aggttatgct attgcttctg ctaatctaaa atttatcata ggctcctctt acctattcct
     1021 catcaattgt agctttattg tcattgcgac ttatataggt gttaggttga tgatggttaa
     1081 gaaacattat tttaaagata atgaagaaga ctctaaaatg cgtaggattt tgcttctagt
     1141 tgctgttttg ctgatgattc cgagtttcat ctctgcaacg actttagtga gagaaacgtt
     1201 gaaaaaagag tcccttaaga aatttatatc agagcagttt caggggcata atattttgaa
     1261 aaaaacctat tctaaaaaga ctcataccct aaagctaacc atttcaggaa attatttgac
     1321 agaagaagaa ctcgatatga tttcaagtaa gagaggtgac tatggtttaa gtgatgtttc
     1381 tgttcaagtt tcacaattgt ctgattcaga acaacttagt aaggaagaac tggtggagta
     1441 tttcttccag tatatcaagg ataaggaagc aaaagaaaag gaaaaagcta ataagtttta
     1501 tacagagtct gaggagcaat aatttcttga gaatagctgg tttttctcgt gagtcttcta
     1561 tgtatatcaa aggaagactg aggttttaag tataaaactt ttcttctatt atagtagcta
     1621 agtcttgaca gggttcaaaa aatggtttac actttataaa gtttattact ttgaaaaggt
     1681 gtgatactgt ggctacggca acaaaaaaga caaaatcaac agttaaaaaa aatctagtca
     1741 tcgtggagtc gcctgctaag gccaagacga ttgaaaaata tctaggcaga aactacaagg
     1801 ttttagccag tgtcgggcat atccgtgatt tgaagaaatc cagtatgtcc gtcgatattg
     1861 aaaataatta tgaaccgcaa tatatcaata tccgaggaaa aggccctctt atcaatgact
     1921 tgaaaaaaga agctaaaaaa gctaataaag tttttctcgc gagtgacccg gaccgtgaag
     1981 gagaagcgat ttcttggcat ttggcccata ttctcaactt ggatgaaaat gatgccaacc
     2041 gtgtggtctt caatgaaatc accaaggatg cagtcaaaaa tgcttttaaa gaacctcgta
     2101 agatcgatat ggacttggtc gatgcccaac aagctcgtcg gatcttggat cgcttggtag
     2161 ggtattcgat ttcgcctatt ttgtggaaga aggtcaagaa gggcttgtca gcaggtcgcg
     2221 ttcagtccat tgcccttaaa ctcatcattg accgtgaaaa tgaaatcaat gccttccagc
     2281 cagaagaata ctggacagtt gatgctgtct ttaaaaaggg aaccaaacaa tttcatgctt
     2341 ccttctatgg agtagatggt aaaaagatga aactgaccag caatgacgaa gtcaaggaag
     2401 tcttgtctcg tctgacgagt aaagactttt cagtagatca ggtggataag aaagagcgca
     2461 agcgcaatgc tcctttaccc tataccactt catctatgca gatggatgct gccaataaaa
     2521 tcaatttccg tactcgaaaa accatgatgg ttgcccaaca gctctatgaa ggaattaata
     2581 tcggttctgg tgttcaaggt ttgattacct atatgcgtac cgattcgact cgtatcagtt
     2641 ctgtagcgca aaatgaggcg gcaagcttca ttacggatcg ttttggtagt aagtattcta
     2701 agcacggtag caaggtcaaa aacgcatcag gtgctcagga tgcccatgag gctattcgtc
     2761 cgtcaagtgt ctttaataca ccagaaagca tcgctaagta tctggacaag gatcagctca
     2821 agctatatac ccttatctgg aatcgttttg tggctagcca gatgacagcg gccgtttttg
     2881 ataccatggc tgttaaattg tctcaaaaag gggttcaatt tgctgccaat ggtagtcagg
     2941 ttaagtttga tggttatctt accatttata atgattctga caagaataag atgttaccgg
     3001 acatggttgt tggagatgtg gtcaaacagg tcaatagcaa accagagcaa catttcaccc
     3061 aaccgcctgc ccgttattct gaagcaacac tgattaaaac cttagaggaa aatggggttg
     3121 gacgtccatc aacctacgcg ccaaccattg aaaccattca gaaacgttat tatgttcgcc
     3181 tggcagccaa acgttttgaa ccgacagagt tgggagaaat tgtcaataag ctcatcgttg
     3241 aatatttccc agatatcgta aacgtgacct tcacagctga aatggaaggt aaactggatg
     3301 atgtcgaagt tggaaaagag cagtggcgac gggtcattga tgccttttac aaaccattct
     3361 ctaaagaagt tgccaaggct gaagaagaaa tggaaaaaat ccagattaag gatgaaccag
     3421 ctggatttga ctgtgaagtg tgtggcagtc caatggtcat taaacttggt cgttttggta
     3481 aattctacgc ttgtagcaat ttcccagatt gccgtcatac ccaagcaatc gtgaaagaga
     3541 ttggtgttga gtgtccaagc tgtcatcagg gacaaattat tgagcgaaaa accaagcgta
     3601 atcgcctatt ctatggttgc aatcgctatc cagaatgtga atttacctct tgggacaagc
     3661 ctgttggtcg tgactgtcca aaatgtggca acttcctcat ggagaaaaaa gtccgtggtg
     3721 gtggcaagca ggttgtttgt agcaaaggcg actacgagga agaaaagatt aaataagagg
     3781 aggggcccga aagtaaattt caggctcctt ttggttagaa cttgacaaaa tacatcattt
     3841 tatgcgaaac tagaagaaga ttattttata ctcagtgaga atcaaagagc aaattagaaa
     3901 gctagtcgca ggttgctcaa agcactactt tgaggttgaa gatggaagct gacctggttt
     3961 gaagagattt tcgaagagta ttaactagga gaagttatgc gtcttatcta tctaattatt
     4021 ggttttttat cgctggcctt ggctattgtt ggggttgttt tacccttgtt acctacaaca
     4081 cctttccttt tgttgtctat tgcttgtttc tccagaagtt ccaagcgatt cgaagactgg
     4141 ctttatcata ccaagctcta tcaaacatat gtagctgatt ttcgcgagac taagtctatt
     4201 acgcgtgaac gaaagaaaaa aatcatcgtc tctatctacg tcttgatggg aatttctatt
     4261 tattttgcac ctcttttacc agtcaaaatc ggtctgggtg ctttgaccat ctttatcact
     4321 tattatcttt tcaaggtcat tccagacaaa gaatagttaa aacagtagtt atttgccttg
     4381 ataaaattga aagcatattc ataacaatat gatataataa atttgaagta atattcaagg
     4441 agaatcaaat gatttacgaa ttttgtgctg aaaatgtgac tttacttgaa aaagcgatgc
     4501 aggctggagc tcgtcggatt gaactctgtg ataatctagc agttggtggg acaacaccca
     4561 gctatggagt gactaaggca gcggttgaac tggcagctaa ctacgataca accatcatga
     4621 ccatgattcg gccacgtggt ggtgactttg tctataatga cctagaaatt gctatcatgc
     4681 tagaagacat ttgtttgact gctcaggctg gaagtcaagg ggttgtattt ggagctttaa
     4741 ctgctgataa aaagttggat aagcctaatc tggaaaagtt aattgctgca tcaaaaggaa
     4801 tggaaattgt ctttcacatg gcctttgatg aactaagtga tgaagatcaa ccggaagcta
     4861 ttgactggct cagtcaagcc ggtgtcactc gtatcctaac tcgtgctggt gtgtctggcg
     4921 actccttaga aaaacgtttt gttcactatc acagaatttt ggagtacgct aaaggtaaaa
     4981 ttgaaattct accaggtggg gggattgacc ttgacaaccg tcaaaccttt attgaccagg
     5041 tgggggtaac acaattgcat ggtactaagg ttgtttttta aaaaatagaa aggaactgct
     5101 agccttgggt agcagttttc acttatgttt gaaattttta aatcctatca atttaatcaa
     5161 gaaaaggctc atgattatgg ttttatagaa aatagcgaag tctggacata tagttgccag
     5221 attttgcaag gtgactttgt catgactgtg tccatcactg ctgataatgt gaactttcaa
     5281 gtctttgacc aagagactgg tgacctttat cctcacgttc atatggaaag catgagggga
     5341 agttttgtcg gaaatgtccg tgaggcttgt ctggagattc tttaccagat tcggaaggct
     5401 tgttttgatg tgcaagattt tatctgtcat cagactaagc gtatcatgac tcaagttcag
     5461 gaaaagtatg gaaaccagtt ggagtatctg tgggaaaaat cgcctgatac agctgtattg
     5521 cgccatgaag gcaatcaaaa gtggtatgcc gtcttgatga aaatctcttg gaataagctg
     5581 gaaaagggca gagaaggaca agtggaagca gtcaacctca agcatgacca agtagctaat
     5641 ttgctttcac aaaaggggat ttatccagcc ttccatatga gcaagcgcta ctggattagt
     5701 gtgtcccttg atgatacttt atcagatgaa gaagtactgg aattgataga aaaaagttgg
     5761 aacttaacct ctaaaaaatg aaatatttta ataattttca tgaactttca attagctaaa
     5821 tattctttac tgaagaaatt ttcagaaaat ataggattta ccacaccaga ggaatgtggt
     5881 gccatcttca aatacctgat tgagaatgtc aagacggatc gtcagatcat ctattcgcct
     5941 cactgtcatg atgacctcgg aatggcagtg gcaaatagcc ttgctgctgt caagaatggt
     6001 gcaggacgtg ttgaagagac tatcaatggt attagggagc gagctgaaaa tgctgctttg
     6061 gaagaaattg cagtggctct caatattcgc caagattact accaagtaga aaccagtatt
     6121 gtcctaaatg agaccatcaa tacgtcagaa atggtttctc gcttctctgg tattccagtt
     6181 cctaaaaaca aagccgtcgt tggtggcaat accttctccc acgaatctgg tattcaccaa
     6241 gatggagtcc ttaaaaatcc tctcacttat gagatcatca cacctgaatt ggttggtgtt
     6301 aagattccgc ttggaaaatt atctggtcgc catgcttttg ttgagaaact gagagaattg
     6361 gccctagatt ttacagaaga ggatatcaaa ccactctttg ctaagttcaa ggcactggcc
     6421 gataagaagt aagaaatcac agatgcagat actcgagctt tggtagctgg aaccatggtt
     6481 gaaaatccag aaggcttcca ctttgatgat ttacaacttc aaactcatgc agataatgac
     6541 attgaagcgc tcgttagcct agccaatatg gatggtgaga aagtcgaatt taatgcgaca
     6601 gggcaaggtt ccgttgaagc aatctttaac gctatcgata agttctttaa ccaatctgtt
     6661 cgtttggtgt cctacactat caatgcggta acagatggaa tcgatgccca ggatcgggtt
     6721 ttggtcactg ttgaaaacag agatacagaa accatcttta atgcagcagg gcttgatttt
     6781 gatgtgttga aggcttctgc tattgcctat ataaacgcta atacctttgt tcaaaaagag
     6841 aatgcaggtg agatgggacg cagtgtttct taccacgata tgcctagtgt gtaaaggaga
     6901 aggctatggc aaagaaaata gtagctctag taggagacgg aattggccca gaaatcatgg
     6961 aggctggttt agaagttctg gaggctctag ctgaaaaaac aggttttgac tatgagattg
     7021 acagacgacc gttcggaggt gcagatattg atgcagcagg acctccctta cctgatgaaa
     7081 cccttaaggc aagtagggaa gcagatgcta tcctactagt agctatcggt agtcctcagt
     7141 atgatggagt agcggttcgc cctgaacaag gcctgatggc tctccgtaag aactcaatct
     7201 ttacgctaat attcgtcctg taaaaatctt tgacagtctc aagtatttgt caccactcaa
     7261 accggaacga atttctggtg tagactttgt cgtggtgcgt gaattgacag gcgagattta
     7321 ctttggagat catattcttg aagagcgcaa agcgcgtgat atcaacgact atagctatga
     7381 ggaagtggag cggattattc gcaaagcctt tgaaattgca agaaatcgca gaaaaatcgt
     7441 tactagtatc gataagcaaa atgttctagc gacctcaaaa ctctggcgga aagtagctga
     7501 ggaagtcgca caggatttct cagatgtaac cttggaacac cagctggtag actcagctgc
     7561 tatgcttatg attaccaatc ctgctaagtt tgatgttatt gtaacggaga atctttttgg
     7621 agatatttta tctgatgaat caagcgtctt atctggtaca cttggggtta tgccatcagc
     7681 cagtcattct gaaaatggac caagtctcta tgaacctatt cacggttcag cacctgatat
     7741 tgcaggtcaa ggaattgcca atcctatttc catgatttta tcagttgtca tgatgttgag
     7801 agatagtttc ggacgttatg aggatacaga gcgtatcaaa cgtgctgttg agacaagtct
     7861 ggcggcagga attttaacga gagatatagg aggtcaggct tcaacaaagg aaatgatgga
     7921 agctattatt gcaaggttat gaagttagac gaaaaaatta ctctagtcct tttgatttgg
     7981 aatgtcatca ttttcttgat ttatggcatt gacaaattca aggcaagaag aagagcttgg
     8041 cgcatcccag agaaaatctt actcatttta gcccttactt gtggtggttt tggggcctgg
     8101 ttagctggaa tcacttttca ccacaagact cgaaaatggt actttaaaac agtttggttt
     8161 cttgggatgg tgaccacact agtagcctta tattatattt ggaggtaatg gatggcaggg
     8221 tcttcgaggg aacacgctgc ttgggctcta gcggactatg gttttaaggt cgtgattgca
     8281 ggatctttcg gtgacattca ttacaataat gaactcaata atggcatgtt gccaatcgtt
     8341 cagcctagag aggttagaga gaaactagcc cagttaaaac caaccgacca ggtaactgtg
     8401 gacttggaac aacaaaaaat catctcacca gttgaagaat tcaccttcga gatagatagc
     8461 aagtggaaac ataaactcct aaatagtttg gatgatatcg gtattacctt gcagtatgaa
     8521 gagttgattg ctgcttatga aaaacaacga ccagcctact ggcaggatta gaaaaaatag
     8581 aaaaggaaat atagtaaact gaaataagat gtaaacaaat gaattggagc ttaacatcaa
     8641 tttccagcaa ttttttagaa actacagtgg actattctgg attcaacaca ttataaaatt
     8701 atgacaaaac acattcacaa gaaggctacg acattttaaa aggtgagggc ggatgtatcg
     8761 tttgccctac taaagttggt tacattatca tgaccagcaa caaggcagga cttgagcgta
     8821 agttcgcagc caaagaacgt aagcgtaaca aaccaggtgt tgttctctgc ggtagcatgg
     8881 atgaactttg cgctttagcg caactcaacc cagaaattga agcattctac taaaaacatt
     8941 gggatgaaga tattcttctt ggttgtatcc ttccttggaa accagaagcc tttgaaaaac
     9001 tcaaagcata cggggatggc cgtgaagaac ttatgactga tgtacgtggt actagctgtt
     9061 ttgttatcaa gtttggaaaa gcaggtgaac aattggctgc caagctttgg gaagaaggta
     9121 aaatggtcta cgcctcatct gcttcaatga caaaacgact gaaactcgct atgagcaagg
     9181 tgtaatggtg tctatggtcg ataaggacgg caaactcatc ccagaacaag gaggagcacg
     9241 ttcaacctca ccagcaccag ttgtgatccg taaagggctt gacattgata tagtaaaatg
     9301 aagtaagatc agcacaaagt aatcaggaca aagtaatcag gacagtcaaa tcgatttcta
     9361 acaatgtttt agaagtagag gtgtactatt ctagtttcaa t
//
LOCUS       DAVKAO010000067         9332 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKAO010000067 DAVKAO010000000
VERSION     DAVKAO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.067"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            279..1088
                     /locus_tag="VWX16_001681"
     CDS             279..1088
                     /locus_tag="VWX16_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9158992.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPRAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGIARAIEKYIL"
     gene            complement(1269..2144)
                     /gene="aguB"
                     /locus_tag="VWX16_001682"
     CDS             complement(1269..2144)
                     /gene="aguB"
                     /locus_tag="VWX16_001682"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9158993.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(2154..3239)
                     /gene="aguA"
                     /locus_tag="VWX16_001683"
     CDS             complement(2154..3239)
                     /gene="aguA"
                     /locus_tag="VWX16_001683"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9158994.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(3236..4363)
                     /gene="nspC"
                     /locus_tag="VWX16_001684"
     CDS             complement(3236..4363)
                     /gene="nspC"
                     /locus_tag="VWX16_001684"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9158995.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWINMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(4363..5622)
                     /locus_tag="VWX16_001685"
     CDS             complement(4363..5622)
                     /locus_tag="VWX16_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9158996.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVY"
     gene            complement(5619..6479)
                     /gene="speE"
                     /locus_tag="VWX16_001686"
     CDS             complement(5619..6479)
                     /gene="speE"
                     /locus_tag="VWX16_001686"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9158997.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFLDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            complement(6510..6647)
                     /locus_tag="VWX16_001687"
     CDS             complement(6510..6647)
                     /locus_tag="VWX16_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9158998.1"
                     /translation="MRYQTHYQLKQKLWLEPFGNRQAIGGNELIHSGLFQVYHLGFGS
                     S"
     gene            6858..7448
                     /locus_tag="VWX16_001688"
     CDS             6858..7448
                     /locus_tag="VWX16_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9158999.1"
                     /translation="MSLRQLNQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYTPLLIK
                     EFAKSLSLRKTKTNKKDAHGIALKLLSDPNREQFQHDNRQVELKILTKHIHRLKKKQS
                     DWKVQYTRCLDIIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTAS
                     NSRAFCRALKSSPNHQIEIRNSLDKTIDFSYSLSFS"
     gene            complement(7414..8889)
                     /locus_tag="VWX16_001689"
     CDS             complement(7414..8889)
                     /locus_tag="VWX16_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9159000.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         2700 a         2092 c         1796 g         2744 t
ORIGIN      
        1 aaatcatgat atatttcctc cattattttt cagattagtt gagtaatctg ctactcatac
       61 ctacttacaa aaaaactatt atattaagtt ggttttttaa tttgtctagt tgcaacattg
      121 acaaacatag tatagcatat ctttgccaac tatcctcttc aaatcatgaa ttgtcatcaa
      181 aacatcttaa actataaaat caattagtct caagctttct atcaatttct tctccaaaat
      241 atgctataat aatagcaaaa gataaagaag gaagacctat gattaaacta ctagccttgg
      301 atatggacgg caccctcctc aatgaagcca aggaaatccc acgagctcac attactgcta
      361 ttcacaaagc cattgaaaaa ggtgtcaaac tggttctctg tacgggtcgc ccccttttcg
      421 gtgtcctccc ctactacaaa aaactgggac tcgacctcca gaatgagtat gtgattgtta
      481 acaacggttg ttcaactcac cagactagcg actggggctt ggttgactgg caagaactta
      541 gtccagctga catcgaatac ctctatgacc ttgctgaaaa gagtgatgtt cagttgacgc
      601 tttttgacga gtcacattat tttgttctcg gtggcaagcc caatcaagtt attgaaaatg
      661 atgctaaact agtattttca gacctgactg aaatttctct tgaagaagcg actagtggaa
      721 agttccggat gttccaaggt atgtttttag gaacaaaaga acaaacagac gattttgagc
      781 agcgttttgc tgaagagctt tgccaacgat tcagtggagt tcgttcgcag cctgtcattt
      841 atgaagcaat gccacttgga acgacaaagg ctactgctct ttcacgacta gctgagattt
      901 tgaagattga ttcctcagag attatggcca tgggcgatgc taataacgat atcgaaatgc
      961 tccagtttgc agggcttggg attgcaatgg gaaatgccag cgattatgtc aaatctcttg
     1021 cggatgccgt tacctcaagc aacgaagaag acggcattgc gcgtgctatt gagaaatata
     1081 ttctataaaa aagaaaagca aatagacaga agttactgct atttgctttt ttgctaatat
     1141 tttaaaatac ggactaatca atagacaaga atagcgaatc ataaaaatcg atttactaga
     1201 tgccatagta tctcatagtt gctaataaga agttagtcta gcagaatgaa tctctcattt
     1261 ctcccacact aatctgtaat ttgtctatac atttctggtc ttctatctcg aaacaagccc
     1321 cagtttaggc gttcacttgc tcccttgtct aggtcataag tagctaacag aacagcttct
     1381 tcttgtcttt cagctcgttc tagaatagct cctgtttcat ccgtcataaa ggaggaaccg
     1441 tagaagtcaa gactggaact ctgtccgcca ttttcctcac taggagtaac ctcctctaaa
     1501 ccataacgat tggctgcgat gactggaaca atattcgctg ctgcgtgccc ttgcatagta
     1561 cgttgccagt gaccacaact atctgtatcc aaaatcggct ctgaaccgat ggctgttgga
     1621 taaaagagca attctgcccc attcagagca agacagcggg ctgtttcggg gaaccattga
     1681 tcccaacaga taccgatacc aatcttagca tagcgagtat tccagacctt gaaaccagtg
     1741 ttaccaggcg tgaaatagaa tttttcttga taataatggt catctggtat gtgggtcttt
     1801 cgataaacgc ccagcacttc cccatctgca tcaatgacgg caatagagtt atacaagaca
     1861 ttaccatctt tttcatagaa actgattggt aaaacaactt gtagttcctt agcaatcacc
     1921 ttaaaatgct gaatggcagt attttccgct acagattggg catactggta gtagtcatac
     1981 tgacgttcct gacagaaata gggatgttca aacaactcgg gcaagagaat aatttgggct
     2041 ccttgctcag cagcctgacg tactaaacgc tctgcggttt ggatatttgt tgccacatcc
     2101 ttagcgcatt gcatctgaat ggttgcaact cttacatttc tcatcttttt ctcctattct
     2161 ggaatttgtt gggtgataca gtggatattg ccaccaccta agagaatatc tctggctggt
     2221 attccgacaa ctttacggtc tgggaaacac ttgctgagga tatctaaggc cacttggtcg
     2281 tttacatcct caaactgtgg aaccaagaca gccttgttgg cgatataaaa gtttacgtag
     2341 gaagctgcta gtcgttcacc tgcgtatcgc tcttcttctc cttcttcata gatgtagcct
     2401 ggcaaatctt cttctgtcac aacttgtcga actgcaggga taggcaattt atgaatggtg
     2461 aagtgacaac cttttgcatc tgtttcctgt tctaagagtt cgagatctgc ttttgacatg
     2521 gcatactggg ggtcattttc gtcatctgtc caagccaaaa caacctcagc aggaccaaca
     2581 aaggcagcaa cattatcgac gtgttcattg gtttcatcct gataaatacc ataaggaagc
     2641 caaataactt tttcagcacc aagactttct aataatgtgt tttcaatctc ctctttagtc
     2701 aggttaggat tgcgaccagg actaagcaag caactttcag ttacgagaat agttccttga
     2761 ccatcgctat ggattgcgcc tccttccagt acaaaaggtt tagcatcata gacaggcctt
     2821 tccaaggcct cagcaaaacg gctggctact tggtcatcct cttcataatc ttgataaaga
     2881 ccatcatagg tgcctcccca agcattgaag gcccaatcca cggctaattt cttaccttta
     2941 tcattgacga gaatggttgg gccagtatca cgcgcccagg catcattggt gggaatgtct
     3001 aaataaacaa ccttgtcccc aagataggat tgggcttcag atagataggc ctgctccacc
     3061 aaaagataga ctctttcccc ttctgctatg gtctcgatga tctgagtaaa tgctctttta
     3121 gcagcctttc cttgaaaagg ccatgatcct ggtcgagtcg gccatatcat gagggtacca
     3181 tgatggggtt cgtactctgc tggcatgtga tagcctaatt tttttggact gtccatcatg
     3241 ataatctccc tttaaagtct tgatagccaa aagctttgag taagctacag tctccctgtt
     3301 cgtccatgag atagagactt ggcaatccaa taccattaaa ggtattattt ttgacaaaag
     3361 aataaatggc catgtcttga aaataaagtc tgtctccgat ttggactgga ttttcaaaac
     3421 tataatcacc aataacatcg cccgtcagac aggtattaga agaaagtctg taggtatggg
     3481 ctttttcctg tgcctcaaag ccatttctca aaggtggacg atagggcatc tcaagtacat
     3541 caggcatatg gcaggtcgca gaggcgtcta aaaccaagat ttccataccg ttttctacaa
     3601 tatctaatac ctcagttgct aaataacccg cattaagcgc aatggcttca ccaggctcga
     3661 tatagatttc aagattgtaa gtttttcgga tacgcttgat ttctgaaatc agcaaatcca
     3721 catcgtaacc ttctcttgta atatgatgac caccacccat attgatccat tttacctcat
     3781 gtaagtaggg accaaactgt tcttctactg ctttcaaagt tgtttgtaaa tcatctgctc
     3841 cctgctcgca aagggtatga aaatgaagtc cgtcaaccaa atctagcaaa tcactcggaa
     3901 tcttgtctat agtaactcca aagcgagaac ctggtgcaca agggtcatag agcgcgtgat
     3961 ctccttgagt tgaacactga gggttgaggc gcaaaccaac actgacacca gcctctcgac
     4021 aacgcgaacc atgcttacgc aactgtctct ctgagttaaa gacgatatgg tctgttatcc
     4081 caagcaattc ctccatgtcc tcatccttga aagctggtgc aaaaacatgg acttcaccag
     4141 gaaattcttc ccttgccaat ttggcctcat agagtccact agctgtcgta cctgatagat
     4201 actggctaat caagggataa gttttgtaga gggaatatgc cttctgggca agcaagacct
     4261 tgcaaccgac ctcttcttgt acatattgta gaatgcggca attagcttct aacttggcca
     4321 agtcaataac ataggctggt gttggtactt gttctaactt cattagtaca ccatttgtgg
     4381 attttcaacc acaacccatg gcaaaccata ctcattcaaa gcttccatga atggatctgg
     4441 atctaactcc tcaaggttat acactccagc ttgtttccaa gttccgttca tgactaattt
     4501 tgtcccaatc atggctggaa cgcctgtcgt gtatgaaatt gcttgtgacc cgacttctgc
     4561 atagcactct tgatggtcgc aaacattgta gatgtagata gtcttttcaa cgccatcttt
     4621 gacacctgta aagatacatc caatattggt ttttccgact gtacgtggcc caagactggc
     4681 aggatctgga agcaaggctt tcaaaaattg aattggaaca atttcttggc cgttaaagtt
     4741 aatggtatcc gtacgaagga gtccaacatt ttcaagacat ttcatgtgcg tcaagtaaga
     4801 ttgaccaaaa gtcataaaga aacgaatgcg tttgacacct ggaatgttct tggccaatga
     4861 ttcgatttct tcatggtgaa ggagatacat atctttttgt ccaacttgag ggaaatcata
     4921 ctcacgcttg atagacatag cttcgacttc gacccatttc ccatcttccc agtaagaacc
     4981 tggcgcagaa acctcacgga gattaatttc tggattaaag ttggttgcaa atggataacc
     5041 gtggtcaccg ccattacagt ctaaaatgtc gatataatgg atttcatcaa aatagtgttt
     5101 gagggcataa gctgaaaaga cactagttac acctgggtca aaaccagaac caagaagagc
     5161 agtcaagcct gcttctttga atttctcttg ataagcccac tgccatgagt agtcaaagta
     5221 ggctgtaaaa ccaagttcct tacaacgttt ttcgtagata gcacgccact cagggtcttc
     5281 tgtgtcttct gcttcgtagt tggctgtatc gatatagtga acacctgttg ccaaacaagc
     5341 atccataatg gttaaatctt gataaggcag agctacattc aaaacagctt ctggtttgta
     5401 gctttcaatc agagcaatca cttcttcaac cttgtcagca tcaagagcag ctgtctcaat
     5461 ctttgtactt gttttgcctt ccagtttagc tttcaagtca tcgcattttg acttggtacg
     5521 gctagcaatc ataatctctg taaatgtttc gctatcttga caaatctttg aaatagcaac
     5581 ttgggcaacg cccccacaac caataactag taaacgactc atttttttcc ttcctcttct
     5641 tctaaaatgt cctcaacata cttgggcaac ataaaggctc ccacgtgtaa gtttgcagtg
     5701 tagtattctg tgaaaagctg gcgttttttc cagccttcct tgtcaaaatc tttgacaggg
     5761 tggtattttt tcgatgcaaa tccaaacaac caatagccag ctgggctagt tggaatatgg
     5821 gcctgataaa cccgactgat tggaaaggct tgattgacct tgcggtgcat gcttcggcag
     5881 gccgactcat cctcgtcaaa gaagggactc ccatgctggt aaatcatgat gccgtcttcc
     5941 ttcagagctc gataactatt gccgtagaat tccttggtaa agagtccttc cgtatggcca
     6001 aatggatctg tcgcatcgtt gatgataata tcgtaatcat cttcgcagtt tcgcaaaaag
     6061 cgtaacccat tttggtagta aatggtaaca cgaggatcat ctagccctgc agcaaagtct
     6121 aggaaatact cacgacagac ctcgaccaac atctcatccg gttctacgat atcgatttgt
     6181 tccagttcag gatagagtgt taatacttgg gcaacaccgc cgtcaccacc cccaataacc
     6241 aatactttct ttggatttgg gtggacagcc atgggaacgt ggacggtcat ttcattgtag
     6301 acgaaatcat ccgcatctga gaacaagaca tggccattta aaatcagtat tttcccaaaa
     6361 gctggcgtat ccaagacttc gatatcctgc cattcacttt ttccagcgta aagttgcttg
     6421 gctgttctca gagacaattt gacatctgga gtatgaactt cagaaaacca taaatccatc
     6481 tatttctcct tatattatat ttagcgcagt tagctactgc caaagcccaa gtggtatact
     6541 tggaataagc cactgtggat tagttcattt cctccaatcg cttgacgatt tccgaaaggc
     6601 tctaaccaca gcttttgttt caattggtaa tgagtttgat aacgcataga cgttttatct
     6661 cgtctaaggt aacaaggcaa aaagtcgtgg ataaccaagt agacttgaaa tactatatct
     6721 ggaggtactc ccatgcttta tgtgggcatt gatgtcgcta aaaataaaca cgatgttaca
     6781 gccttgaatg ttccaggaaa actgttctta aaccactcac tttttcaaat aataaagctg
     6841 gttttgaact cttagatctg tctcttcgac agctcaacca agactgtctc atcgctcttg
     6901 aagatactgg acattatgcc tttaatctac tgaacttcct gcatgaacaa ggatataaag
     6961 tttacaccta cactcctttg ctcatcaagg aatttgccaa atcattatca cttcgcaaga
     7021 ctaaaacgaa taagaaagac gctcatggca tcgctcttaa acttctttct gaccccaatc
     7081 gtgaacaatt tcaacacgat aatcggcaag tagaactaaa aatactgact aaacatattc
     7141 atcgtctcaa gaaaaaacag tctgattgga aagtacaata cactcgttgt cttgatatca
     7201 tctttcctga gttggataaa atcgttggaa agcattcaga atatacctac caactcttga
     7261 cacgctaccc taatcctcag aaaaggattg aggcaggatt tgataagctg atagaaatta
     7321 agcgattgac cgcttctaat tcaagggctt tttgccgtgc tctaaaatca tcgccaaatc
     7381 atcaaattga aataagaaat agtttagata aaactattga cttttcttat agtttgtctt
     7441 tctcttaata acgttgatat gattgacctc tggatcttcc gtcccttgga gggagcaacc
     7501 acgttccttg gcgaattgga tatagtcgac aatttctcgt gtaatgcgtt caccaggagc
     7561 caagatagga atacctggag ggtaacacat aacaaattct ccacagacct gtccaacaga
     7621 atcatccaaa gttaaacttt ttctttctga atagaaggct tcttgcggag acagcactaa
     7681 ctcgggctga atatattctc ctgctatcaa atcttttcca tctcttgaat agagtctctt
     7741 aatatcagcc agagcaccaa ccaagcgctc gatgtcttgg atgcggtcgc cgatggaaat
     7801 ataggccaag atattgccga tatcaccaaa ctcgatctga atgtcgtatt cgtctcgcaa
     7861 gaggtcataa acctcgatac ctgttaagcc aataccctga gtgtaaactg acagcttagt
     7921 tacgtcaaaa tcgcaaaccg aaacaccgtc tattaactct tttgagtagg catagtagcc
     7981 accgatagca ttgatttcac ggcgggcata ctcagatagc tcaatgactt tctcaaacga
     8041 ctctttacca cgaagggcca agttgcgacg tgaaatatcc aaactagcca tcaacaagta
     8101 agaggcagat gtagactggg tcagattaat tatctgacga acgtattcag aattcatctg
     8161 ctccccgata agtaaaatgg agctttgggt caaactccca ccagacttat gcatggaaac
     8221 tgctgccata tcagcccctg catccatagc agaaattgga agtttatcag tgaaatgcaa
     8281 atgcgctccg tgggcttcat ctactaaaac catcatgcca gcttcatgag ccatttctgt
     8341 caaccccttt aggtctgaac agatgccgta gtaagtagga ttgttgatta ggatagcctt
     8401 agcatctgga tggtccttta tggcctgtgc tactcggtca ttttcaagac ctaaagcgat
     8461 accaatctta ggatctacac tcatctcgat atagatggga atggcaccac atagaaccaa
     8521 cgcattgata gcagatttat ggacatttcg tggcagaata atcttatctc ctgccttgca
     8581 ggttgccaga atcatagtct gcaccgatga agttgttcca ccaatcatta gaaaggcatg
     8641 gctagctcca aaagcatctg cagccagctc ctctgcatca cgaataatcg aaataggatg
     8701 gcctaaatta tccaagggtt tcattgaatt gacatcaatg cctacacatt tttctcctaa
     8761 gagttcgaca agttctggat ttccccgtcc acgcttgtga cctggaacat caaagggaac
     8821 aatccttttc ttgcgtaact tcaccaaggc ctcataaatt ggggcttggt tttgatctaa
     8881 ctctttcaaa acatactcct ttcaatattt ttaggacctt atgaaaacta aggaactaaa
     8941 ggttgactca tgaaaaaaga gcaggttttc acctgctctg caatcttctg acgtgtttat
     9001 tttggtttct gttgagtatg tctgttcccg atgtttccta actctctagt agcaaatatt
     9061 acgtacttta ttgatcgttt cacaagatga cgtgtgcttt caccacacta aaaatttatg
     9121 aattaggaca agtgtcctaa catcaactta taaaagtagg cttttaaccc tatcaataat
     9181 gtggcttttc aaccacgctt cggcattcaa ccctgcctgt ccgtaggcat tttttactcg
     9241 ggtttatatt tgctagaaaa catcatctca ttttctaagc tttattacta tatcatgttt
     9301 ttaagaactt gtaaagtatt ttttgataaa aa
//
LOCUS       DAVKAO010000068         9220 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKAO010000068 DAVKAO010000000
VERSION     DAVKAO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.068"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            76..1557
                     /locus_tag="VWX16_001690"
     CDS             76..1557
                     /locus_tag="VWX16_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9159001.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1919..2920
                     /locus_tag="VWX16_001691"
     CDS             1919..2920
                     /locus_tag="VWX16_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9159002.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3071..3988
                     /locus_tag="VWX16_001692"
     CDS             3071..3988
                     /locus_tag="VWX16_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9159003.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3999..4889
                     /locus_tag="VWX16_001693"
     CDS             3999..4889
                     /locus_tag="VWX16_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9159004.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFIAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4919..6535
                     /locus_tag="VWX16_001694"
     CDS             4919..6535
                     /locus_tag="VWX16_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9159005.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6545..7864
                     /locus_tag="VWX16_001695"
     CDS             6545..7864
                     /locus_tag="VWX16_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9159006.1"
                     /translation="MGDKKYTVEKANSFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            8261..8674
                     /locus_tag="VWX16_001696"
     CDS             8261..8674
                     /locus_tag="VWX16_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159007.1"
                     /translation="MKNNERLGIKLSRDSVLGLREVRRLYLGSSDIPVPDSYVIEVAY
                     KQISNEIDIIDWVELNKSKIKISEISESVDIDATSLRTTLTLDTLVYEGMRDIQLKLR
                     ELTKGRVFFSFVVKLVLFASILKKKDLLEKFQEKC"
     gene            8925..>9220
                     /locus_tag="VWX16_001697"
     CDS             8925..>9220
                     /locus_tag="VWX16_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159008.1"
                     /translation="MKQKQPIVSRTKQHTFEELIQDQKLERLANLSPDLVGRYGFTAS
                     CASSFANLIKEAYGGKNLNVVYASRMLALWNIACSCYHKADGYSLADALFSDKK"
BASE COUNT         3099 a         1400 c         1779 g         2942 t
ORIGIN      
        1 tcaggctata cctattcttt gtggtataat tgcaagaggt ttaatccgat aatttataaa
       61 gagagaaaga tattcatgag agatttatta tctaaaaaaa gtcataggca attagaatta
      121 ttagaattac tatttgaaca taaacgttgg tttcatcgtt ctgaactagc agagttatta
      181 aattgtacag aacgtgcagt caaagatgat ctatcccatg ttaaatctgc ttttcctgac
      241 ttgatttttc attcttctac taatggtata cgcattatta ataccgatga tagtgatatt
      301 gaaatggttt accatcattt ctttaaacat tcaactcatt tttcgatttt agaattcatc
      361 ttctttaatg aaggttgtca agctgagagt atttgtaaag aattttatat cagttcatct
      421 tcgctctatc gtattattag ccaaatcaat aaagtgatta aaaggcaatt tcaatttgaa
      481 gtcagtctga cccctgttca aatcattgga aatgagagag acattcgtta cttttttgca
      541 caatattttt cagaaaaata ttatttccta gaatggccat ttgaaaattt ttcatcagag
      601 ccactatctc aattgttaga attggtttat aaggaaacaa gctttccaat gaatttgtca
      661 acgcatagaa tgctaaagtt gctcctagtt acgaacctat atagaataaa gtttggtcat
      721 tttatggaag tagataaaga ttcttttaac gaccaaagtt tggatttttt aatgcaggca
      781 gaaggaatag aaggtgttgc tcagagtttt gaatcagaat acaatatctc tttagatgaa
      841 gaagttgttt gccaattatt tgtgtcttac tttcaaaaaa tgtttttcat agatgaaagt
      901 ctctttatga aatgcgtaaa aaaggatagt tatgttgaaa aatcttacca tctattgagt
      961 gattttattg atcagatttc agtcaagtat cagattgaaa tggagaataa ggataatctg
     1021 atttggcatc tgcataatac cgcacatctg tatcgtcaag agttgtttac tgagtttatt
     1081 ttatttgatc aaaaagggaa tacaatcagg aactttcaaa atatttttcc aaaatttgtt
     1141 tcagatataa aaaaagagct ttctcactat ttagagactc ttgaggtttg ttctagttca
     1201 atgatggtaa atcatctatc ttataccttc atcactcata ccaaacattt ggtgattaat
     1261 ttgctacaaa atcagcctaa actgaaggtt ttagttatga gtaattttga tcagtatcat
     1321 gcaaaattcg ttgcagagac actttcttat tactgtagca ataattttga actggaagtt
     1381 tggactgaat tagaattatc aaaggaatct ttagaagatt catcttatga tatcattatt
     1441 tctaatttta ttattcctcc tattgaaaat aagagactca tctattcaaa taatataaac
     1501 acggtctcac tcatatattt gttaaatgcc atgatgttta ttcgattaga tgagtaacca
     1561 aaaataacaa aaaaacgcta tttcgcgttt gaactgtacc tcaaaagtta gacagaaaaa
     1621 atctaatttt tggggtgttt ttgttaagaa aagaccttca atcatttaag caaaatgtac
     1681 atctagattt atagccctct aaaactcttg ctttttttgt agaagatacg aaaaaagcca
     1741 taaaaccaag gcttttcctg ttttatgtag atgtttacac atgaaaattg aagtattttc
     1801 aaaagctaat tggagtgatt ttattcatga tgggataaat gatctggcca aaaatgattg
     1861 agaaatagtt gcttaaaaag catttgcatg atactatata ttgaaaggag gtgataatat
     1921 ggtagaacaa agaaaatcaa ttaccatgaa agatgttgct ttagaagcag gagttagtgt
     1981 tggaactgtt tcacgtgtaa ttaataaaga aaaaggcatt aaagaagtaa ctttgaaaaa
     2041 agtggaacaa gcgattaaaa ctttgaatta cattccagat tactacgcta gaggaatgaa
     2101 aaaaaatcga acagaaacga ttgcaatcat tgtaccaagt atctggcatc ccttcttttc
     2161 agaatttgct atgcatgtgg aaaatgaagt ctataagaga aataacaaat tactcttatg
     2221 ttctatcaat ggtacaaata gagagcaaga ctatctggag atgttgcgtc ataataaagt
     2281 tgatggagtg gttgccatta cctatagtcc aattgaacat tacttgacgt caggaattcc
     2341 ctttgttagt attgaccgca catactcaga tattgccatt ccttgtgttt catcagataa
     2401 tgatgcaggt ggaagagaag cagctaaaca attaattagt aaaggatgtc agcatttagc
     2461 ctttgtgggc ggacataata caaccattaa tgaaaccaaa cgtcgtagaa tttcatttga
     2521 aaagtatgtc caagagcata aaatacctct tagtattttt gatttggatg agacagttac
     2581 tgactattat ggaaagctag agagtttctt ggaagaaaat tcttctatag atggaatttt
     2641 tacaatcaac gattttacag ctttggatgt gattgaaata ctagaaaaaa atggtaaacg
     2701 tattcctcat gatgtccaaa ttattggata cgatggaatt aaaatggctg gagatagaga
     2761 ttatctactt tcaactattg aacaaccatt ggaagaaatg gcaaaagaag cggttcgtat
     2821 tttgtttgat ataattgatg gaaagactgt taatttgcag acaatcctac cagtaaaatt
     2881 tgttgaagga aaaacaacaa aaaatgaaaa taagtcttga cagaaagcgc tatcaatgat
     2941 agaataaatt cagataaaaa agatttattt ttaaaacaaa aatgaaacgt ttcaaaaaag
     3001 aaataaagag acagcgctaa gcgttatctt ttctagaaaa aaatgaaacg tttcaaaaaa
     3061 ggaggttgct atgaatagca aagcgaagca agtttctctt tgggaaagaa tcaagaaaca
     3121 aaaactcttg ttattgatga ctgtccccgg tttagtttta acctttatct ttaaatacat
     3181 ccctatgtat ggggttttaa tcgcatttaa agattacaat cctttaaaag gaattttagg
     3241 gagtgattgg attggttttt ctgagtttac aaaattcata tcctctccca actttggtat
     3301 cttgttagcc aacacattaa aattaagtat ctatggttta ttgcttggct ttttaccacc
     3361 aatcattctt gctattatgc tcaatcaact cttgagtgaa aaagtcaaaa aacgaattca
     3421 gctcatttta tacgcaccaa actttatctc agttgttgtt attgtcggta tgattttcct
     3481 cttcttttca gtgggaggac caatcaacaa ttttctttct atgtttggaa tgaaggctga
     3541 cttcttgaca aatccagact tctttagacc tttatacatc tttagtggta tctggcaagg
     3601 aatgggctgg gcttcaacgc tctacacggc aacattggta aatgtagatc cagccttagt
     3661 agaagcagcc cgactggatg gagccaatat cttccaacga atctggcaca ttgatattcc
     3721 agctcttaag cctattatgg ttatccaatt tgttttagct gcaggtggaa ttatgaatgt
     3781 cggatatgaa aaagcattct tgatgcagac atcgttaaat ttgccaactt ctgaaattat
     3841 ctcgacatat gtctataaag ttggtcttgt atcaggagac tattcttact caacagcggt
     3901 tggtttgttt aatgcagtga ttaacgtagt attgcttgtt gcagttaacc aaatcgttaa
     3961 acgcatgaat aatggtgaag gaatttaagg aggaaagtat gaaaaattcg attatggata
     4021 caaaatttga tagacgtatc ttactcttaa ataaaatcat tattgtcttt atcgttttga
     4081 tgactttgct tcctttactt tatatcgtcg tagcatcctt tatggatcct aaggttctgg
     4141 ttagtagagg gattagcttt aatccagccg attggactgt agaaggttac cagcgtgtat
     4201 tcagtgacca atctattcta agaggtttta tcaattctct actatactct tttggatttg
     4261 cagctttaac agtattgcta tctgtgttta tagcttatcc tctttctaag aaagacttgg
     4321 ttggacgtcg ttggattaac tacttcttga ttgtgactat gttctttggt ggtggtttag
     4381 tcccaactta cttgctcgta aaagaattgg gaatgctcaa tactccatgg gctatcattg
     4441 ttccaggtgc tgtcaacgtt tggaatatta ttcttgctag ggcctatttc caaggattgc
     4501 ctgaagaatt agttgaagct gctgtcattg atggtgcaaa tgatttacag attttcttca
     4561 aaatcatgct tcctcttgca aaaccaatta tgtttgttct cttcctttat gcttttgtag
     4621 gacagtggaa ctcatacttt gatgcaatga tttatatcaa ggatccaaac ttggaaccat
     4681 tgcaacttgt acttcgtaaa attctcattc agagccaacc aggtcaagac atgattggag
     4741 cacaagcggc tatgaatgaa atgaaacgtt tagctgaatt gattaaatac gcaactattg
     4801 tcatttccag cttgccattg attgttatgt atccattctt ccaaaaatac tttgataaag
     4861 gaattatggc tggttcactt aaaggataaa aaaaagaaaa aataaaagga gttttctcat
     4921 gaaattcaaa acattctcaa aatcagcagt tttgttgaca gctagtttag cagtacttgc
     4981 agcctgtggc tcaaaaaata cagcttcaag tccagattat aagttggaag gtgtaacatt
     5041 cccgcttcaa gaaaagaaaa cattgaagtt tatgacagcc agttcaccgt tatctcctaa
     5101 agacccaaat gaaaagttaa ttttgcaacg tttggagaag gaaactggcg ttcatattga
     5161 ctggaccaac taccaatccg actttgcaga aaaacgtaac ttggatattt ctagtggtga
     5221 tttaccagat gctatccaca acgacggagc ttcagatgtg gacttgatga actgggctaa
     5281 aaaaggtgtt attattccag ttgaagattt gattgataaa tacatgccaa atcttaagaa
     5341 aattttggat gagaaaccag agtacaaggc cttgatgaca gcacctgatg ggcacattta
     5401 ctcattccca tggattgaag agcttggaga tggtaaagag tctattcaca gcgtcaacga
     5461 tatggcttgg attaacaaag attggcttaa gaaacttggt cttgaaatgc caaaaactac
     5521 tgatgatttg attaaagtcc tagaagcttt caaaaacggg gatccaaatg gaaatggaga
     5581 ggctgatgaa attccatttt catttattag tggtaacgga aacgaagatt ttaaattcct
     5641 atttgctgca tttggtatag gggataacga tgatcattta gtagtaggaa atgatggcaa
     5701 agttgacttc acagcagata acgataacta taaagaaggt gtcaaattta tccgtcaatt
     5761 gcaagaaaaa ggcttgattg ataaagaagc tttcgaacat gattggaata gttacattgc
     5821 taaaggtcat gatcagaaat ttggtgttta ctttacatgg gataagaata atgttactgg
     5881 aagtaacgaa agttatgatg ttttaccagt acttgctgga ccaagcggtc aaaaacacgt
     5941 agctcgtaca aacggtatgg gatttgcacg tgacaagatg gttattacca gtgtaaacaa
     6001 aaacctagaa ttgacagcta aatggattga tgcacaatac gctccactcc aatctgtgca
     6061 aaataactgg ggaacttacg gagatgacaa acaacaaaac atctttgaat tggatcaagc
     6121 gtcaaatagt ctaaaacact taccactaaa cggaactgca ccagcagaac ttcgtcaaaa
     6181 gactgaagta ggaggaccac tagctatcct agattcatac tatggtaaag taacaaccat
     6241 gcctgatgat gccaaatggc gtttggatct tatcaaagaa tattatgttc cttacatgag
     6301 caatgacaat aactatccaa gagtctttat gacacaggaa gatttggaca agattgccca
     6361 tatcgaggca gatatgaatg actatatcta ccgtaaacgt gctgaatgga ttgtaaatgg
     6421 caatattgat actgagtggg atgattacaa gaaagaactt gaaaaatacg gactttctga
     6481 ttacctcgct attaaacaaa aatactacga ccaataccaa gcaaacaaaa actagaggtt
     6541 gattatggga gataagaaat acacagtaga aaaagccaat agttttatag cagaaaataa
     6601 acatctcgtt aatactcaat ataagcctga agaacatttt tcagctgaga ttggttggat
     6661 caatgatcca aatggatttg tctattttcg tggagaatac catctctttt atcaattcta
     6721 tccatatgat agtgtttggg ggcctatgca ctggggacat gctaaaagta aggacttggt
     6781 gacttgggag cacttgccag tggcacttgc tcctgaccaa gattatgacc gaaatggttg
     6841 tttctcaggc tctgccattg tcaaggatga tcgcctctgg ctcatgtaca ctgggcatat
     6901 cgaagaaaaa accggtgtcc gccaagtgca aaatatggca ttttcagatg acgggattca
     6961 ctttgaaaag atttcccaaa atccagttgt aactggaaca gacttgccag atgagttgat
     7021 tgctgctgat ttccgtgatc caaaactctt tgaaaaagat ggacgctatt actccgtagt
     7081 agctgccaaa cacaaggata atgtgggctg tatcgttcta ctagggtccg ataacctagt
     7141 agaatggcag ttcgaatcca tctttttaaa agggggagaa caccaaggct ttatgtggga
     7201 atgcccagat tacttcgagt tagatgggaa agattgcctt attatgtcac ccatgcgtta
     7261 tcagcgtgag ggagactcat atcataacat caactcatcg cttttgttca cgggtaaggt
     7321 agattgggga gaaaaacgtt ttatcccaga atcagttcaa gaaattgatc atggccaaga
     7381 cttctatgcg cctcaaacat tgttggacaa tcaaaatcgt cgtatcctga ttgcttggat
     7441 gcagacatgg gggcgtaccc ttccaaccca tgaccaagaa cacaaatggg catgtgccat
     7501 gactttacct agaattctaa gattggaaga tggcaaacta agacaattcc ctgttaaaaa
     7561 aggccaatat caaatccaaa tagataaaga ttgtcattac cacttaggaa atgatataga
     7621 ttatcttgaa tttggttatg acagtaatgc gcagcaagtt tacattgatc gtagccatct
     7681 tattcaaaaa attctaggtg aagaagaaca ggacactagt cgacggtatg tagatattga
     7741 agctaaagaa ttggaagttg ttctagataa aaattccatc gagatttttg tcaatcaagg
     7801 tgaagcaagc ttgactgcaa cttattactt aacggtgcca gctgagctat cacgaattga
     7861 ttaaaaatta agttatttct cctaaagaaa aagttctctt tctaaaatag tggaaagagg
     7921 actttttgtg ttttgggtat ataagcttag tttatggtat ttgtaaaatt ggtgttggat
     7981 tatgatttaa actagttttc taaagaattt gaaaaaaatt ttatttaagc aaaaaaagcc
     8041 ctatattata agacttttcc tgttgtattt aaatgccccc tacaggaatt gtaggagata
     8101 tgttgtttag atgttcttga ttttctggtg ttttgtaacg tttaaatgag ttttttgagt
     8161 ttgttggtgg ggcgttgccc ggcaattgcc cgacttattg tttgaaaaag aatttaaaat
     8221 atggtatagt taattataga ttaacacttg gggggaatag atgaagaata atgaaagatt
     8281 aggtattaaa ttaagtagag atagcgtttt aggattgagg gaagttagaa ggctttattt
     8341 aggcagttca gatatcccag ttcctgatag ctatgtgatt gaagttgctt ataaacagat
     8401 atcaaatgag attgatatta ttgattgggt agagttgaac aagtcaaaaa ttaagataag
     8461 tgaaattagt gaaagcgtgg atatagatgc cactagcttg agaacaactt tgactttaga
     8521 cacattagta tatgaaggta tgagagatat acagttaaag ttgagagagc ttacaaaggg
     8581 gagagtattc ttttcatttg tagtgaagtt agttttgttt gcttctattt taaagaaaaa
     8641 agatttacta gaaaaatttc aagaaaagtg ttaatcaagt attgacactt tatctggatt
     8701 tcggtataat atgcttagaa aggaatcttt ctaaattttt ttcgtcctta tgtgttaatc
     8761 aaagacgaat acaaaaacat atttttttac tctaaaaagt gttaatcaat gatgtatttg
     8821 ttagagaggt agataaatgg aattgagagc accaccagtt ataacagtat aaaacgtata
     8881 ataaaaatat tttaacttga attatagaaa aggagaaaca aatcatgaaa caaaaacaac
     8941 cgattgtttc tagaacgaaa caacatacat ttgaagagct tattcaagac caaaagttag
     9001 aaagattggc taatttgtcg cccgatttgg ttggaaggta tggttttact gctagctgtg
     9061 cgtcttcatt tgcgaacttg attaaagaag cgtatggggg taaaaatcta aacgtagttt
     9121 atgcgagtcg gatgttggct ctctggaata ttgcttgcag ttgttatcat aaggctgatg
     9181 ggtattcttt agcagatgcg ctttttagtg ataaaaaaat
//
LOCUS       DAVKAO010000069         9004 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKAO010000069 DAVKAO010000000
VERSION     DAVKAO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.069"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            1..213
                     /gene="secG"
                     /locus_tag="VWX16_001698"
     CDS             1..213
                     /gene="secG"
                     /locus_tag="VWX16_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9159009.1"
                     /translation="MLLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERSKARGFEA
                     VMQRLTGILVFFWLAIALALTVLSSR"
     gene            315..2669
                     /gene="rnr"
                     /locus_tag="VWX16_001699"
     CDS             315..2669
                     /gene="rnr"
                     /locus_tag="VWX16_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9159010.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKKYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGESYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2632..3099
                     /gene="smpB"
                     /locus_tag="VWX16_001700"
     CDS             2632..3099
                     /gene="smpB"
                     /locus_tag="VWX16_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9159011.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKRQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            3115..3975
                     /gene="tehB"
                     /locus_tag="VWX16_001701"
     CDS             3115..3975
                     /gene="tehB"
                     /locus_tag="VWX16_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9159012.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEVMETVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            4048..5001
                     /gene="coiA"
                     /locus_tag="VWX16_001702"
     CDS             4048..5001
                     /gene="coiA"
                     /locus_tag="VWX16_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9159013.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            5020..6822
                     /gene="pepF"
                     /locus_tag="VWX16_001703"
     CDS             5020..6822
                     /gene="pepF"
                     /locus_tag="VWX16_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9159014.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            6824..7537
                     /locus_tag="VWX16_001704"
     CDS             6824..7537
                     /locus_tag="VWX16_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9159015.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            7604..8545
                     /gene="prsA"
                     /locus_tag="VWX16_001705"
     CDS             7604..8545
                     /gene="prsA"
                     /locus_tag="VWX16_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9159016.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(8664..>9004)
                     /locus_tag="VWX16_001706"
     CDS             complement(8664..>9004)
                     /locus_tag="VWX16_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9159017.1"
                     /translation="LEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRK
                     GNSQDNGRMESFFGILKSEMFYGYEQTFKSLNQLEQAIIDYIDYYNNKRIKIKLKGLS
                     SVQYRTKSFG"
BASE COUNT         2885 a         1660 c         1978 g         2481 t
ORIGIN      
        1 attttattag tattatctgt tgtgattgtg attgcaattt tcatgcaacc aaccaaaaac
       61 caatccagca atgtatttga tgccagttca ggtgatttgt ttgaacgcag taaagctcgc
      121 ggttttgaag ctgtaatgca gcgtttgaca gggattttag tctttttctg gctagccatt
      181 gccttagcat tgacggtatt atcaagtaga taagaaaata atgggcagga ctaggtcttt
      241 gcctcttttt atttttaaag gatgtttgag aaggttttac agtaaaagaa aattaaaaaa
      301 tctagaaaga aaatatgaaa gatagaataa aagaatattt acaagacaag ggaaaggtga
      361 ctgttaatga tttggctcag gctttgggaa aagacagttc caaggatttt cgtgagttga
      421 ttaaaacctt gtccttaatg gaaagaaagc accaaattcg ttttgaagaa gatggtagtc
      481 tgacattaga aattaagaaa aaacatgaga ttaccctcaa ggggattttt catgcccata
      541 aaaatggctt tggctttgtt agtctggaag gcgaggagga cgaccttttt gtagggaaaa
      601 atgatgtcaa ctatgctatt gatggtgata ccgtcgaggt agtgattaag aaagtcgctg
      661 accgcaataa gggaacagca gcagaagcca aaattattga tatcctagaa cacagtttga
      721 caacagttgt cgggcaaatc gttctggatc aggaaaaacc taagtatgct ggctatattc
      781 gttcaaaaaa tcagaaaatc agtcaaccga tttatgttaa gaaaccagcc ctaaaattag
      841 aaggaacaga agttctcaag gtctttatcg ataaataccc aagcaagaaa catgatttct
      901 ttgtcgcgag tgttctcgat gtggtgggac actcaacgga tgtcggaatt gatgttcttg
      961 aggttttgga atcaatggac attgtatccg agtttccaga agctgttgtt aaggaagcag
     1021 aaagtgtgcc tgatgctccg tctcaaaagg atatggaagg tcgtctggat ctaagagatg
     1081 aaattacctt taccattgac ggtgcggatg ccaaggactt ggacgatgca gtgcatatca
     1141 aggctctgaa aaatggcaat ctggagcttg gggttcacat cgcagatgtt tcttattatg
     1201 tgaccgaggg gtctgccctt gacaaggaag cccttaaccg tgcgacttct gtttacgtga
     1261 cagaccgagt ggtgccaatg cttccagaac gactatcaaa tggcatctgc tctctcaatc
     1321 cccaagttga ccgcctgacc cagtctgcta ttatggagat tgataaacat ggtcgtgtgg
     1381 tcaactatac cattacacaa acagttatca agaccagttt tcgtatgacc tatagcgatg
     1441 tcaatgatat cctagctggc gatgaagaaa agagaaaaaa atatcataaa attgtaccaa
     1501 gtatcgaact catggccaag cttcatgaaa ctttagaaaa catgcgtgtg aaacgtggag
     1561 ctctcaattt tgataccaat gaagcgaaga ttttagtgga taaacaaggt aagcctgttg
     1621 atatcgttct tcggcagcgt ggtattgccg agcggatgat tgagtctttt atgttgatgg
     1681 ctaatgaaac agttgccgaa catttcagca agttggattt gccttttatc tatcgaattc
     1741 acgaggagcc taaggctgaa aaggttcaga agtttattga ttatgcttcg agttttggct
     1801 tgcgcattta tgggactgcc agtgagatta gtcaggaggc acttcaagac atcatgcgtg
     1861 ctgttgaggg agaatcttat gcagatgtat tgtccatgat gcttcttcgc tctatgcagc
     1921 aggctcgtta ttcggagcac aatcacggcc actatggact agctgctgac tattatactc
     1981 actttaccag tccaattcgt cgttatccag accttcttgt tcaccgtatg attcgggatt
     2041 acggccgttc taaggaaata gcagagcatt ttgaacaagt gattccagag attgcgaccc
     2101 agtcttccaa ccgtgaacgt cgtgccatag aagctgagcg tgaagtcgaa gccatgaaaa
     2161 aggctgagta tatggaagaa tacgtgggtg aagagtatga tgcagttgta tcaagtattg
     2221 tcaaattcgg tctctttgtc gaattgccaa acacagttga aggcttgatt cacatcacta
     2281 atctacctga attttatcat ttcaatgagc gtgatttgac tcttcgtgga gaaaaatcag
     2341 gtatcacttt ccgagtgggt cagcagatcc gtatccgtgt tgaaagagcg gataaaatga
     2401 ctggagagat tgatttttca ttcgtaccta gtgagtttga tgtgattgaa aaaggcttga
     2461 aacagtctag tcgtagtggc agagggcgtg gttcaaatcg tcgttcggat aagaaggaag
     2521 acaagagaaa atcaggacgc tcaaatgata agcgtaagca ttcacaaaaa gacaagaaga
     2581 aaaaaggaaa gaaacctttt tacaaggaag tagctaagaa aggagccaag catggcaaag
     2641 ggcgagggaa aggtcgtcgc acaaaataaa aaggcacgcc acgactatac aatcgtagat
     2701 acgctagagg cagggatggt cctgactgga actgaaatca agagtgtacg agctgctcga
     2761 attaatctca aggatggctt tgctcaagtg aaaaatggag aagtttggct gagcaatgtt
     2821 catatcgcgc cttacgaaga gggcaatatc tggaaccagg aaccagaacg tcgtcgtaaa
     2881 ctcctgctcc ataaaaggca aattcaaaaa ttggaacaag agaccaaagg gacaggaatg
     2941 accttagttc cccttaaggt ctatataaaa gatggctacg ctaagcttct tttaggactt
     3001 gccaaaggga agcatgacta tgacaaacgg gagtctatca aacgtcgtga gcaaaatcga
     3061 gatatcgcgc gtgtgatgaa agctgttaat cagcgataaa aagaggaatt gaaaatggaa
     3121 aaattagttg cctataaacg catgcctttg tggaataaac aaacaatgcc tgaagctgtt
     3181 cagcaaaagc acaatacaaa agttgggact tgggggaaaa ttactgtctt gaagggagct
     3241 ctcaagttta ttgaattgac agaagaaggg gaagttctag ctgaacacct ctttgaagca
     3301 ggggcagaca atccaatggc ccaacctcaa gcctggcacc gagtggaagc tgccacagat
     3361 gatgtggaat ggtacttgga attttattgt aaacctgagg attattttgc taaaaaatac
     3421 aataccaatc ctgttcattc agaggtccta gaggtcatgg agacagtgaa acaagggaaa
     3481 gctttggatt tgggttgtgg tcaggggcgt aattctcttt ttctagccca gcaagatttt
     3541 gatgtgacgg ctgtagatca aaatggacta gctcttgaaa tcttgcaaag cattgtggag
     3601 caggaagatt tggacatgcc tgttggcctt tacgatatca attcagctag cattgaacaa
     3661 gaatatgatt ttatcgtttc aacagttgtt ctcatgtttc tacaagcgga ccgcattcca
     3721 gctattattc aaaatatgca ggagaaaacc agtgttggtg gttacaacct tatcgtttgt
     3781 gccatggaca cggaggatta tccttgctcg gttaacttcc cattcacctt taaagaagga
     3841 gaactggcag actattacaa ggattgggaa ttggttaagt acaatgaaaa tccaggccat
     3901 ttgcatcgtc gcgatgagaa tggcaatcgt attcaactac gctttgcgac cttactagct
     3961 aagaaaatca agtaaacaca catgaagatt aggaattttc ctgatctttt ttctttttta
     4021 cgaatgatat agaaaaggag ggaattcatg tttgttgcga gagatgctag gggagaattg
     4081 gtaaatgtgt tagaggataa acttgagaag caagcataca cctgcccagc ttgtggaggc
     4141 cagctccatt tgcgtcaagg accaagtgta cggacgcatt ttgcccataa atccttaaaa
     4201 gactgtgatt ttttctttga aaatgaaagt ccagaacacc tggccaataa ggaatccctc
     4261 tatcactggt tgaaaaaaga gacaaaggtt caattagagt acccgctttc agaacttaaa
     4321 cagattgcgg atgtatttgt aaatggcaat ctagctctag aagttcagtg tagtcccttg
     4381 cctcagaaag tccttaaaga gcgaagtgag ggctatcgta gtcagggtta ccaagtactg
     4441 tggttgctgg gtcaaaaact gtggctcaag gagcgtttga ctcgtctaca gcaaggtttt
     4501 ctttatttca gtcaaaacat gggcttttat gtttgggaat tagacaagga aaaacaagtt
     4561 ttaagactca aatacctgat ttaccaggat ctccgcggta aactccatta tcaaatcaag
     4621 gaattttcct atggtcaagg tagtttattg gaaatattgc gtcttcccta taagagacaa
     4681 aaaatatctc attttacagt ttctgaggac aaggacatct gtcgctatat ccggcaacaa
     4741 ctttattatc aaaatctctt ttggatgaaa gaacaagcag aagcctatca aaagggagaa
     4801 aatatcctga cttatggact gaaagaatgg tatccacaaa ttcgaccaat agtgggcaaa
     4861 tttttccaga ttgaacaaga cttgactagc tattatcagc acttttatac ctattaccaa
     4921 aaaaatcctc aaaatgattg gcaaaagctt tatccaccag ccttttatca gcaatatttc
     4981 ttgaaaaata tggtagaata gaaaggatgg aggaatctaa tggtattaca aagaaatgaa
     5041 ataaatgaaa aagatacatg ggatctatca acgatctacc caactgacca ggcttgggaa
     5101 gaagccttaa aagatttaac agaacaattg gagacagtag cccagtatga aggccatctc
     5161 ttggatagtg cggataacct actagaaatc actgaatttt ctcttgaaat ggaacgccag
     5221 atggagaagc tttacgttta tgctcatatg aagaatgacc aggatacacg tgaagctaag
     5281 tatcaagagt actatgccaa ggccatgaca ctctacagcc agttagacca agccttttca
     5341 ttctatgatc ctgaatttat ggagattagc gaaaagcagt atgctgactt tttagaagct
     5401 caaccaaagc tgcaggttta tcaacactat tttgacaagc tcttgcaagg caaggatcac
     5461 gttctttcac aacgtgaaga agaattattg gctggagctg gagaaatctt tggttcagca
     5521 agtgaaacct tcgctatctt ggacaatgcg gatattgtgt tcccttatgt cctagacgat
     5581 gatggtaaag aagttcagct atctcatggg acttacacac gtttgatgga gtctaaaaaa
     5641 cgtgaggttc gccgtggtgc ctatcaagct ctttatgcga cttacgaaca attccaacac
     5701 acctatgcca aaaccttgca aaccaatgtt aaggtgcaaa attaccgtgc taaagttcgt
     5761 aactacaaga gtgctcgtca tgcagctctc gcagcgaatt ttgttccaga aagtgtttat
     5821 gacaatttgg tagcagcagt tcgcaagcat ttgccactct tacatcgcta tcttgagctt
     5881 cgttcaaaaa tcttggggat ttcagatctc aagatgtacg atgtctacac accgctttca
     5941 tctgttgaat acaattttac ctaccaagaa gccttgaaaa aagcagaaga tgctttggca
     6001 gtcttgggtg aggattactt gagccgtgtc aaacgtgcct tcagcgagcg ttggattgat
     6061 gtttacgaaa atcaaggcaa gcgttcaggt gcctactctg gtggttctta cgataccaat
     6121 gcctttatgc ttctcaactg gcaggacaat ctggacaatc tctttactct tgttcatgaa
     6181 acaggtcaca gtatgcattc aagctatact cgtgaaactc agccttatgt ttacggagat
     6241 tactctatct ttttggctga gattgcctca actaccaatg aaaatatctt gacggagaaa
     6301 ttattggaag aagtggaaga cgacgcaaca cgctttgcta ttctcaataa cttcctagat
     6361 ggtttccgtg gaacagtttt ccgccaaact caatttgctg agtttgaaca cgccattcac
     6421 caagcagatc aaaatgggga ggtcttgaca agcgatttcc taaataaact ctacgcagac
     6481 ttgaaccaag agtattatgg tttgagtaag gaagacaatc ctgaaatcca atacgagtgg
     6541 gctcgcattc cacacttcta ctataactac tatgtatatc aatattcaac tggctttgcg
     6601 gccgcctcag ccttggctga aaaaattgtc catggtagtc aagaagaccg tgaccgctat
     6661 atcgactacc tcaaggcagg taagtcggac tatccactta atgtcatgag aaaagctggt
     6721 gttgatatgg agaaggaaga ctacctcaac gatgcctttg cagtctttga acgccgttta
     6781 aatgagtttg aagcccttgt tgaaaaatta ggattggcat aaaatggttg aatcgtatag
     6841 taagaatgct aaccataaca tgcgtcgtcc tgtcgtcaaa gaagaaattg tagacttgat
     6901 gcgtcagcgt caaaagcagg tcacaggttt cttgaaagaa ttggaagact ttgcccgcaa
     6961 ggaaaatatt cctattattc cccatgaaac ggttgcttat ttccgttttc ttatggaaac
     7021 catgcagcct aaaaatattc tggaaattgg gacggctatc ggtttttcag ctctcttgat
     7081 ggctgaacat gcgccaaatg ctaagattac aactattgat cgtaatccag aaatgattgg
     7141 ttttgccaag gaaaattttg cccagtttga cagtcgcaag caaatcactc tcctagaggg
     7201 agatgcggtg gatgtcttat ctacactgac agagtcttat gatttcgtct ttatggattc
     7261 tgccaagtct aaatacatcg tctttctgcc agaaatcctc aaacatttgg aagttggtgg
     7321 tgtggttgtc ttggatgata tttttcaagg tggtgatgtt gccaaggata ttatggaagt
     7381 ccgtcgtggt cagcgaacca tttatcgagg ccttcaaaaa ttatttgatg caaccttaga
     7441 caatccagaa ctcaccgcaa cattagtgcc tttaggagat ggtattctca tgcttcgtaa
     7501 aaatgtagca gatgttcaac tgtctgaaag cgaatgattt tcagaaaaat ttaagaaaaa
     7561 atagtaaaat agatagagta acacttatct caaaggagta gacatgaaga aaaaattatt
     7621 ggcaggtgcc atcacactat tatcagtagc aactttagca gcttgttcga aagggtcaga
     7681 aggagcagac cttatcagca tgaaagggga tgtcatcaca gaacatcaat tttatgagca
     7741 agtgaaaaac aacccttcag cccaacaagt cttgttaaat atgaccatcc aaaaagtttt
     7801 tgaaaaacaa tatggctcag agcttgatga taaagaggtt gatgatacta ttgccgaaga
     7861 aaaaaaacaa tatggcgaaa actaccaacg tgtcttgtca caagcaggta tgactcttga
     7921 aacacgtaaa gctcaaattc gtacaagtaa attagttgag ttggcagtta agaaggtagc
     7981 agaagctgaa ttgacagatg aagcctataa gaaagccttt gatgagtaca ctccagatgt
     8041 aacggctcaa atcatccgtc ttaataatga agataaggcc aaagaagttc tcgaaaaagc
     8101 caaggcagaa ggtgctgatt ttgctcaatt agccaaagat aattcaactg atgaaaaaac
     8161 aaaagaaaat ggtggagaaa ttacctttga ttctgcttca acagaagtac ctgagcaagt
     8221 caaaaaagcc gctttcgctt tagatgtgga tggtgtttct gatgtgatta cagcaactgg
     8281 cacacaagcc tacagtagcc aatattacat tgtaaaactc actaagaaaa cagaaaaatc
     8341 atctaatatt gatgactaca aagaaaaatt aaaaactgtt atcttgactc aaaaacaaaa
     8401 tgattcaaca tttgttcaaa gcattatcgg aaaagaattg caagcagcca atatcaaggt
     8461 taaggaccaa gccttccaaa atatctttac ccaatatatc ggtggtggag attcaagctc
     8521 aagcagtagt acatcaaacg aatagtccaa atcaatgagt cagggaaaaa actcgacttc
     8581 aggaaaaaat gaagcaaaca ttcccacaat aaaacgcata gtacaaggtt tgtactgccc
     8641 cccaaaaaag ttagacaatt aatttatccg aaggatttag ttctgtattg cacagagcta
     8701 agtcctttta gttttatctt aattctctta ttgttgtaat aatcaatata gtctataatg
     8761 gcttgttcca attgattaag tgatttaaat gtttgctcat agccataaaa catttcggat
     8821 tttaaaatgc caaagaaaga ttccatccta ccgttgtctt ggctgtttcc tttgcgtgac
     8881 atagatgctt gaattccctt actctctagg aagcgatgat aagaatcgtg ttggtattgc
     8941 cagccttggt cactatggag aatcgtattc tcgtagtgct tctctttgaa tgcctgttcc
     9001 aaca
//
LOCUS       DAVKAO010000070         8960 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKAO010000070 DAVKAO010000000
VERSION     DAVKAO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.070"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(327..1175)
                     /gene="dprA"
                     /locus_tag="VWX16_001707"
     CDS             complement(327..1175)
                     /gene="dprA"
                     /locus_tag="VWX16_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9159018.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLALSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDIFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1261..1950)
                     /locus_tag="VWX16_001708"
     CDS             complement(1261..1950)
                     /locus_tag="VWX16_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9159019.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1962..2840)
                     /locus_tag="VWX16_001709"
     CDS             complement(1962..2840)
                     /locus_tag="VWX16_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9159020.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2830..3699)
                     /gene="licA"
                     /locus_tag="VWX16_001710"
     CDS             complement(2830..3699)
                     /gene="licA"
                     /locus_tag="VWX16_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9159021.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3716..4738)
                     /locus_tag="VWX16_001711"
     CDS             complement(3716..4738)
                     /locus_tag="VWX16_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9159022.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKGCYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGCIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4743..5450)
                     /locus_tag="VWX16_001712"
     CDS             complement(4743..5450)
                     /locus_tag="VWX16_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9159023.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5786..7273
                     /gene="tacF"
                     /locus_tag="VWX16_001713"
     CDS             5786..7273
                     /gene="tacF"
                     /locus_tag="VWX16_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9159024.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7283..8086
                     /locus_tag="VWX16_001714"
     CDS             7283..8086
                     /locus_tag="VWX16_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9159025.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8088..8897
                     /locus_tag="VWX16_001715"
     CDS             8088..8897
                     /locus_tag="VWX16_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9159026.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2811 a         1807 c         1516 g         2826 t
ORIGIN      
        1 ctatactttc ccttatactc aatgaaaatc aaagcgcaaa ctaggaagct agccacaggt
       61 tgctcaaagc actgctttga ggttgtagat aagactgacg aagtcagctc aaaatactgt
      121 tttgaggttg cagatggaag ctgacgtagt ttgaagagat tttcgaagag tattatatga
      181 aaaaatcctc tactctacta atccacttag aaaaaacgca tattatcaag tgttaaaaac
      241 ttgctaatat gcgttttatt atggaaatgt tgggttcatt ttctcttaaa attgattttt
      301 tcttagaatt ctagcttagg tcatttttaa aattcaaatt ccgcaagaac atcttgccca
      361 ctggtgacca attttgctcc ttcttgaatc aaatgatggc aaccgtctga tagtccatct
      421 aaaatgctac caggaatagc aaagatatcg cgtccttctt ccattgctcg ctcacaggta
      481 atgagactac ctgaacgcat cttagcctct gctacaatca caccacgaca aagtccagca
      541 atgatgcgat tacgggcagg aaaatgaaat ttcagaggtt gttcgccagg tccatattca
      601 cttagagcca gatggtcatt gccgatgtag tcttgcaagc gtttgttggc tttaggataa
      661 aacacatcca gtcctgttcc aatcactgca atggtttttc cgccattctg aagagctgcc
      721 atatgagctg ctgtgtcaat gcccttggcc agaccactga caataaccag ttcattttcc
      781 aagccttgaa tgactttttc aactgactta gctccctgtt tgctacaagc acgactgccc
      841 acgaccgcta ccttcgggaa tttcaggagg tcaagatttc ccttgtaaaa taaaagtaca
      901 ggcgcatcat atatttcact caaatcccaa ggataacagt catctaaaat agagaaagat
      961 ggaaattttt gaaactcttt cgacaaatgc gcatcgtcta tctgaaaata acgttccata
     1021 aaaacggctg gattacggca acctgagata tctgcaatat cacccaacaa aagctcctga
     1081 tcaacatttt caccgtattc tagcactttc aaaatctgtt gattggtcaa acctgatttt
     1141 tttaacttat agatttcata gtttgtgatt ttcataaata actccatttc tttttctact
     1201 catctatcta ttcgtaaaaa atcaaaaaac atccgactat tttgtcagat gttggaatct
     1261 ttaattttcg tttttaagaa tttcttctaa tttacgatag tcttggacac tatcgatctc
     1321 ataaatgcta ttgccttcta attcttcaac atagacatct agctctttga tattatcctt
     1381 aaccatattg tcccagtaga gatcaacaaa ttcaccactt gcataagcct tgtcgataaa
     1441 gctgacaatc ttttctgcag ttggagcatc ccagaaggat acaccactaa ggatgcgacc
     1501 tgccttgcta tcaacaataa tgtcttgaac cttgtagtca tctccataga ccaagaacca
     1561 ttcgttggta caatcttcac gataaacact aaaataagtc gaacgtgtca aatcattgcg
     1621 gaacatattt ttaaagagat aattgtcagc atcaataaca tagctgttgg ccaattcttc
     1681 ttttacaaga tagagagagt aaaagttatt gtagtcagcg tatttatcat tgaaaacgag
     1741 gcgaacaccg tatttttctt ttaaataatc gaattgttct ttaagataac caacgatgat
     1801 gatgatgtca ttgattcctt tttctttgag aaactcaatt tggtattcaa tcaaaggttt
     1861 ttgattaacc tgaaccaagg ctttaggggt attttcagtc ataggacgca agcgagttcc
     1921 caatcccgct gctaagataa tggctttcac acgaatctcc tttattcttt aataataata
     1981 taaactccag caatgacgac aagtgacgtc ataatggtca gcatatctaa tgatgttccc
     2041 aagaaaagag ctgcgaacaa gacagtccat actacatagc tcacgttcaa gcctgtagcc
     2101 ttggctggtt gcaagcgatt gatagcgata taataagcca aataggaaat catatcaaag
     2161 gctacaaaga caatcatgag acctagcaat tgtccattgg ctactgcaac aaatgactga
     2221 tgagagaaga gcacaatcac aagataggac aagaacgaag ttacttgacg gattaagagg
     2281 gcttcgattt cactcaattc actttccatg gcaaaagagc taagaacact ctcacttccc
     2341 catgcaatag cacaaaccaa agcacaaaga atcccaatgt agaaagaatt aacctgttca
     2401 accttatagg tctgggcaat aatccctcca ataatcaaga caatcccaaa tacagtattt
     2461 ttcgaaatct tgtgcttcaa aaagaagaaa gccaatagaa ctgaaatcgc agggtaaata
     2521 gccgatacag atgaagctaa agaacttcca atatacttaa ctgcataaag attggcctgc
     2581 ataccgatag ggcctgctag caaggctcct atgataacac tgacattgcg aatatttaag
     2641 aaaattgaga ggcgaacttt cccttctttt accaagagaa aagctagtaa gataaagatg
     2701 ctcaaaaaat catgagttgc agccaccaca aagggtgaca aatctgtaaa aatcgaaaag
     2761 atataagcac taaccgttag acccaagccc cagaaaatac ctgagagaag gccaaaagga
     2821 actccgtttt tacttttcat ctgaacctcc ataagaagcc aaacctttaa tagctctttg
     2881 gtaacgattc acaccatagt caccaaaatc ttcaccttgc tcttccttat agacagtcca
     2941 tagactccaa atagtatctt gtaaaatttt ataaatagca atcttttcat gagaaaccgg
     3001 tgtttggtca ctctcatagt gagataagaa agtttcttcc tcttgggaag tgaattcaga
     3061 ctctaaaaag agggcagcca aatcccacat tggatcattc attgatgaat attcccagtc
     3121 aatcaaataa agtcgtcctt gaggtgattc gataaagttt tcaggcacca aatcgatatg
     3181 acaagatttt ctgtcaacac ctaagtcagc cagtcttttc tctaaggaga agactgcatt
     3241 tctaacagat tcatagttgg cataaggaat ttgttcttca atcaaggatt cgtatttttt
     3301 gatttcttca aaaggagcaa attctcctct taattcctta gcagacgtat gaatagtttg
     3361 taatattgga gtaattttgt cgaacttggt cttgattgac gttgaatcaa gcgtaatcgc
     3421 agattcgata tactcattta ctttgatacc agcttcaata tcaaaaagat aattttttac
     3481 atctaagcct aaatccttta gtagttcaag attgtacttt tcatcttgtc gattgataag
     3541 cttttctgtc cctttaccaa agaatttaac aatgtattgc ttatttgttg ttttggccaa
     3601 atagttttga ttggtcattc cacccagttg ttcaacactg aggacttcct cttcttgact
     3661 aagtaaggaa gaaatttttt ctttaatgat tttctccaca attaacctcc agtacttata
     3721 cttcccactt aaacactgtt ttaaaggctg tgtttaaatc ggttgcaaag acacgatgaa
     3781 tatctttaat ttctcttaca ggttcttcta gataaaggat atttttaaga cgattggcaa
     3841 atttcttgac ttccatcatt tggatagcat tttcaaaatc aatgcaacca gaacgagatg
     3901 acccaaccaa aatcaagccc ttttctaagg catcgcgagt attgagattg actttatatt
     3961 cgctaactcc catcatgaga atcgttccct gaggacgaat gtagcgaatc aagtcattaa
     4021 tagctggtcc agtaccatca ccaccacaac attcaaaagc atggtcaaag gccaaatctt
     4081 caggaatatt atccgtaata tagcatcctt tggcaaatga gaagagttcc aacttttccc
     4141 aatgacgacc aataaccaca atctctgctt ctggcaaagt atagttgata atattggcaa
     4201 ccacaaaagc taaactccca tctccaataa cggcgatccg ctcccgcttg ctatgagcaa
     4261 gagtcaatag acgattcata gcgtgcatgc ccacactgac aaactctgta atggctgcaa
     4321 ccgtatcttc aatagcatca taagccacca cacgatcttt agggagagaa acaaactctc
     4381 tcataaagcc atcaaatcca ctagacaaga aatgggtccc tgtcatgtag ttttcataga
     4441 attcttcatc actctgcata ggagactgat tgggaatcat gacaactttt tgaccaacct
     4501 cgtaggttcc ggtcgggtca gaaatgacgg ttccacatga ctcgtgaatc attgccattg
     4561 gaagcttttt attcaaaatc ttgggatcac gttttccctg atagtaacgc tgatccgcat
     4621 gacagacagc catgtagttg ggacggataa ggatatgatt ctcttggtca atagcctctt
     4681 cctgatattt gacattgata aacttaggct tagttagttg ataaatttga ttaatcattt
     4741 tactagtctt tctcaatcat actttttgca atcttcaaat ctgttacggt tgtaatcttc
     4801 agatttgagt attcaccttt ggccaaggcc acatcttttc ctttgatcac aaagatttta
     4861 catgcatctg tcaagatttc cttctcttca tcagaaagag atccataaag gtccatgaag
     4921 tccttgcaac ggaatgtttg aggtgtttgt ccttgataaa ggtgagcacg atttggaata
     4981 tctgtaataa attgaccatt ggtactttca acgatagtat caaccgcttc taccactgtg
     5041 tccactgcgt catgattttg ggcaagttgg atattgtcct gaatcatgcg aagtgtaata
     5101 aatggacgaa cagaatcgtg ggtaacaacg atatcctctg gagtaagcgg acgataagca
     5161 tcaatggctt caatgatttt cttaatactt gtattgcggt cagcaccacc ctttgtaatg
     5221 atgatacgtt ccttataaag aggaagatat ttatctacaa gatcttctgc atgagaaacc
     5281 cagtctccat gaacaccaac tacaattttt tcaatacttg gctccaagac aaatttttca
     5341 attgtatgaa tcaaaatagg tcgatcacct agctctaaaa attgttttgg caagttactg
     5401 atccccatgc gtgtgccagt tccaccggca agaattcctg cataaatcat ctattttctc
     5461 ctttgtctta ctaaaaaaac ttattctctc tattatacca caatctagtc ctatcataaa
     5521 agaaatactg gtcctcccct tctagaatag gagaattatg cagttctatt attcaaagaa
     5581 actagtccat ttctagtaat gactaggaat tctgtagcaa ttactaataa atggctgcga
     5641 aattttagag tccttcagaa taatatactt tccttaaaga aaacttaaaa cttaagattt
     5701 gaaacttcaa agtgattact tgacttactg cccttcattt cttattagct aactttatga
     5761 tataatgaaa aacgaggtaa attgaatgaa aagtataaaa ttaaatgctc tatcttacat
     5821 gggaattcgt gtcttgaata ttatttttcc catcctaact ggaacctatg tcgcgcgtgt
     5881 cttggaccga actgactatg gttacttcaa ctcagtcgac actattttgt catttttctt
     5941 gccctttgca acttatggtg tctataacta cggtttaagg gctatcagta atgtcaagga
     6001 taacaaaaaa gatcttaaca gaaccttttc tagtcttttt tatttgtgca tcgcttgtac
     6061 gattttgacc actgctgtct atatcctagc ctatcctctc ttctttactg ataatccaat
     6121 cgtcaaaaag gtctaccttg ttatggggat tcaactcatt gcccagattt tttcaatcga
     6181 atgggtcaat gaagctctgg aaaattacag ttttctcttt tacaaaactg ccttcatccg
     6241 tatcctgatg ctggtctcta ttttcttatt tgttaaaaat gaacacgata ttgttgtcta
     6301 tacacttgtg atgagtttat cgacgctgat taactacctg attagttatt tttggattaa
     6361 aagagacatc aaacttgtta agattcacct aagtgatttt aaaccactct ttctccctct
     6421 gacagccatg ttagtctttg ccaatgccaa tatgctcttc acttttttag atcgcctctt
     6481 cctcgttaaa acagggattg atgtcaacgt tagttactat accatagctc agcgaattgt
     6541 gaccgttata gctggggttg taacaggtgc aattggagtg agtgtgcctc gtctcagtta
     6601 ctatctgggg aaaggagaca aagaagccta tgtttctctg gttaatagag gtagtcgaat
     6661 ctttaacttc tttatcattc cactgagttt tggactcatg gttttaggac caaatgccat
     6721 cctactttac ggtagtgaaa aatatatcgg aggcggcatc ttgacctctc tcttcgcttt
     6781 tcgtacgatt atcctggcct tagataccat tcttggttcc caaattctct ttaccaatgg
     6841 ctatgaaaaa cgtatcacag tctatacagt ctttgctggg ctactcaatt tgggcttgaa
     6901 tagtctcctc tttttcaacc atatcgtagc tcccgaatac tacttactga caactatgtt
     6961 atcagaggct tctctacttg ttttctatat cattttcatc catagaaaac aactcatcca
     7021 cttgggacat atctttagct atactgttcg atactctctc ttttcacttt cctttgtagc
     7081 aatttatttc ctgattaatt tcgtgtatcc tgtggatatg gtcattaatt tgccattttt
     7141 gattaatact ggtttgattg tcttgttatc agctatctct tatattagtc tacttgtctt
     7201 cacaaaagat agcattttct atgaattttt aaaccatgtc ctagccttaa aaaataaatt
     7261 caaaaaatca taggagttta aaatgaaaca actaaccatt gaagatgcca aacaaattga
     7321 attagaaatt ttggattata ttgatactct ctgtaaaaag cacaatatca actatattat
     7381 taactacggt actctgattg gggcggttcg acatgagggc tttatccctt gggacgacga
     7441 tattgatctg tccatgccta gagaagacta ccaacgattt attaacattt ttcaaaagga
     7501 aaaaagcaag tataagctcc tatccttaga aactgataag aactacttta acaactttat
     7561 caagataacc gacagtacga ctaaaattat tgatactcga aatacaaaaa cctatgagtc
     7621 tggtatcttt atcgatattt tccctataga tcgctttgat gatcctaagg tcattgatac
     7681 ttgttataaa ctggaaagct tcaaactgct gtctttcagt aaacataaaa atattgtcta
     7741 taaggatagc cttttaaaag attggatacg aacagccttt tggttgctcc ttcgaccggt
     7801 ttctcctcgt tattttgcaa ataaaatcga gaaagaaatt caaaaatata gtcgtgaaaa
     7861 tgggcagtat atggctttta ttccttcaaa atttaaggaa aaggaagtct tcccaagtgg
     7921 tacctttgat aaaacaatcg atttaccctt tgagaattta agccttcctg cacctgaaaa
     7981 atttgatact attttgacac aattttatgg agattatatg accctaccac cagaagaaaa
     8041 acgcttctac agtcatgaat ttcacgctta taaattggag gattaggatg caatatttag
     8101 aaaaaaaaga aattaaagaa attcaactag ccctgctgga ctatattgat gagacttgta
     8161 agaaacatga tattccttat tttctcagtt atggaaccat gcttggagcc atccgccaca
     8221 aaggtatgat tccttgggat gatgatattg atatttccct ttatcgtgag gattatgagc
     8281 gtttactgaa gattattgaa gaagaaaatc accctcgcta caaggttctt tcctacgata
     8341 catcttcttg gtacttccat aatttcgcat cgattttgga cacttctact gttatagaag
     8401 accatgttaa gtacaagcgt catgatacca gccttttcat cgatgtcttc ccaattgatc
     8461 gatttacaga cttgagcatt gtcgacaaga gctataagta tgtggctctt cgtcaactag
     8521 cttatatcaa aaaatcacga gcagttcacg gtgatagcaa actaaaagat tttcttagat
     8581 tatgtagctg gtacgctctc cgatttgtca atcctcgcta cttttacaag aaaattgatc
     8641 aactagtcaa aaatgctgta accaacactc ctcaatatga aggaggagtt gggatcggta
     8701 aggaagggat gaaagaaatc ttcccagttg atacctttaa agaactgatt ttaactgagt
     8761 ttgagggccg tatgttgcct gttcccaaaa aatatgacca atttttaacc cagatgtatg
     8821 gcgattatat gacaccacca tcaaaagaaa tgcaagagtg gtatagtcat agcattaaag
     8881 cttatcgcaa aaactgattg agggggatta tacagactac taagatagag gttattcaaa
     8941 aacataattt tagtagaaaa
//
LOCUS       DAVKAO010000071         8772 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKAO010000071 DAVKAO010000000
VERSION     DAVKAO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.071"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            233..1174
                     /locus_tag="VWX16_001716"
     CDS             233..1174
                     /locus_tag="VWX16_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9159027.1"
                     /translation="MLTYFVTLKIIYYFHLELAYFDKDYLYPFLKKVFFALLLLHIVS
                     FVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQ
                     NYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLRSDK
                     YTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLL
                     NNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKGRNRGV
                     GLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            1171..1923
                     /locus_tag="VWX16_001717"
     CDS             1171..1923
                     /locus_tag="VWX16_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9159028.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            1975..2046
                     /locus_tag="VWX16_001718"
     tRNA            1975..2046
                     /locus_tag="VWX16_001718"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2008..2010,aa:Glu,seq:ttc)
     gene            2052..2125
                     /locus_tag="VWX16_001719"
     tRNA            2052..2125
                     /locus_tag="VWX16_001719"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2086..2088,aa:Asn,seq:gtt)
     gene            complement(2165..2707)
                     /locus_tag="VWX16_001720"
     CDS             complement(2165..2707)
                     /locus_tag="VWX16_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9159029.1"
                     /translation="MQESNKRLKIKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDKN"
     gene            2885..3652
                     /locus_tag="VWX16_001721"
     CDS             2885..3652
                     /locus_tag="VWX16_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEU9159030.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            3737..4075
                     /locus_tag="VWX16_001722"
     CDS             3737..4075
                     /locus_tag="VWX16_001722"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159031.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFFAAISTNMIFAKLPSGRH
                     PESRWAWLKS"
     gene            4103..4417
                     /locus_tag="VWX16_001723"
     CDS             4103..4417
                     /locus_tag="VWX16_001723"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9159032.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(4518..7070)
                     /locus_tag="VWX16_001724"
     CDS             complement(4518..7070)
                     /locus_tag="VWX16_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9159033.1"
                     /translation="MKSFLKTYRTYFISFIIPIVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVIFFTFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLKFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(7132..8754)
                     /locus_tag="VWX16_001725"
     CDS             complement(7132..8754)
                     /locus_tag="VWX16_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9159034.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         2916 a         1391 c         1619 g         2846 t
ORIGIN      
        1 atatattcta tatttagatg ggttagggat tgcaacattt ttatttcctt tgggcctata
       61 ttcctatttt cgatggataa aacagtatga gagggataga ggactattcc taagtttact
      121 actatctctt ttatatgaga gcactcataa ctttctgccc gtaactttct cctctataac
      181 aggagataat tttgttttac aatatcattt cccattcttt ttcgttgtga cggtgttgac
      241 ctattttgtt acattaaaaa tcatttatta tttccatttg gaactagcct attttgacaa
      301 ggactacctt tatcctttct tgaaaaaagt attttttgct ttactattgc tacatattgt
      361 atctttcgtt tcagatatgg taagtacgat taaacatttg aatagttttg gaagtatttt
      421 gtcatctatt gtctttatct ctctcctttt gaccttcttt gcaatgaatt ctcataaagt
      481 tcaaatggag aaagagattg ctttgaagca gaagaaattt gaacagaaac atttacagaa
      541 ttacacagat gaaattgttg gtctgtataa tgaaatccgt ggttttcgac atgattatgc
      601 tggaatgctt gtcagcatgc agatggcaat tgacagtggt aatttacagg aaattgacag
      661 aatttacaat gaagttttag tcaaagcaaa tcataaattg cgttcagata agtacactta
      721 ctttgatttg aacaacatag aagactcagc tttacgaagt ttggttgctc agtcaattgt
      781 ctatgctcga aataatggtg tagagtttac actggaagta aaagatacga ttaccaagct
      841 tccaattgaa ctattggatt tggttcgtat catgagcgtt ttattgaata atgctgtcga
      901 aggatcggct gatagctata aaaagcagat ggaagtagca gttattaaga tggaaactga
      961 aacagttatt gtgattcaga attcatgtaa aatgacgatg actccttcag gagatctatt
     1021 tgccttagga ttctccacta agggaagaaa tcgcggagtc ggattaaata atgtgaaaga
     1081 actactagat aagtacaaca atattatttt agaaacagag atggaaggca gtacatttag
     1141 acaaatcatt agatttaaga gggaatttga atgaaagttt taattttaga agatgttatt
     1201 gaacatcaag tgagactaga gagaatattg gatgaaattt cgaaagaatc gaatattcca
     1261 atatcataca agacaacggg aaaagtccgt gaatttgaag aatacattga aaatgatgaa
     1321 gtaaatcaac tttatttcct agatatcgat attcatggaa ttgagaaaaa gggatttgaa
     1381 gtggctcagc tcattcgtca ttacaatcct tacgctatta tcgtctttat cactagtcga
     1441 tcagagtttg cgactctaac ctataaatac caggtatcag ccctagattt tgttgataag
     1501 gatatcaatg atgagatgtt taagaagaga attgagcaaa atatcttcta cacgaagagt
     1561 atgttacttg aaaatgaaga tgttgtagat tatttcgact acaattacaa gggaaatgat
     1621 ttaaaaattc cttaccatga tattttgtat attgaaacaa caggggtctc tcataaattg
     1681 cgcattattg gtaagaattt tgcaaaagag ttttatggta ccatgacaga tattcaggaa
     1741 aaggacaaac atactcagcg attttattct cctcacaagt catttttggt aaatataggc
     1801 aatatcagag aaattgatcg aaaaaactta gaaattgttt tctatgaaga ccatcgttgt
     1861 cctatttcaa gattaaaaat tagaaaatta aaagatattt tagagaaaaa atctcaaaag
     1921 tgattgacaa ttagcaagaa attgatataa tggttatatc tgctacagat agaaggtccg
     1981 ttggtcaagg ggttaagaca ccgccttttc acggcggtaa cacgggttcg aatcccgtac
     2041 ggactatttt atccgccata gctcagttgg tagtagcgca tgactgttaa tcatgatgtc
     2101 gtaggttcga gtcctactgg cggagtatag atagaaggga acacagctgt gttccctttt
     2161 tgtatcaatt tttatcacca agcattttca taaggaagtc tgttatttct tgagaacttt
     2221 ctttttttcc atgtgcaatc caagtttggc agacaccaaa aagtgcatga gttagataga
     2281 tgctactata ttctaattcg gtggtattta gattcagttg cataaatcgc ttttgtaaat
     2341 ctgtactaag catgatatga agtttatttc gtaagaaatt ttggatttct ttagtcccat
     2401 tttcagaaag aagggcagcc agaagtggtt ctgactctag atattcaaaa acttctaaaa
     2461 tagcgtctct tttgtgatga gcatgttttt gaaaaatata ttcaaatgta tggaatagct
     2521 tgctttgata gtgctcaatc atatcatact tatccttata gtgagtatag aagctggaac
     2581 gactaattcc ggctttttct actaatttga cagtagaaat tttatcaaat ggctgttcca
     2641 tcagtaattg taccatagca ttttcaatag ttcgctttat ttttaagcgt ttgttacttt
     2701 cttgcatatt tcctccttgt aaacaaatta gactatatgt ctaaaaatag attttttatc
     2761 ttgtaattta gattttttaa tgtataatct attatatcaa aattttagac aatatgttta
     2821 aaaaaggaga aactaagttt aaagaatgga aagcaattta aaaaaaacaa cctttattat
     2881 tgtcatgatc gggatttctc ttattccaga tctgtacaat atcatatttt tgtcatcaat
     2941 gtgggatcca tatgggcaat tgtctgactt acctgtggca gttgtaaata atgataaaga
     3001 ggcttcctat aatggtaata ctatggcaat aggaaaagac atggtgtcca atttaaaaga
     3061 aaataaaacc ttggattttc attttgtaga tgaagaggaa ggaaagaagg gattggaaga
     3121 tggcgattac tatatggtag tgactttacc aagtgattta tctgaaaaaa caactacatt
     3181 atccaatatt caatcgacag cagcttatca atcattgaca agtgagcaac aaactgagat
     3241 aagtgattct gtatctcaaa attcaactga tagtattcaa tcggctcagt caattgtagc
     3301 tttagtacaa gatttacagg gaagtttaga aaacttacaa aatcaatctt ctaatctttc
     3361 gactttaaaa aatcaatcta atcaagtatc acctattact tctacttctt tgataggatt
     3421 gtcaagtgga ttaacagaga tacaaggaga tgttactagc aaattagttc ctgccagtca
     3481 gtcgattgca tcaggtgtaa acgcatatac tacaggtgtt gataaagttt ctcagggcgc
     3541 aagtcaacta agtgaaaaaa atgccacctt gacaggtagt ttggataaac tagtttcagg
     3601 ctcaaacacc ttgacacaaa aatcttctag attgacagca ggagttggtt aattacaatc
     3661 aggatctggg caattagcag acaaatccag tcagttactt tcaggtgctt ctccattaga
     3721 gaatagagct aataaattgg cagatggatc tgggaaacta gcagaaggtg gaacaaagtt
     3781 aacttctgga ttggaagatt tacagacagg acttgcttct ttaggacaag gactaggtaa
     3841 tgctagtgat caactcaaat cagtatcaac agaatctaaa aatgcagaga ttttgtcaaa
     3901 tccactcaat ctttcaaaaa cagacaatga tcaagttcct gtaaatggaa tcgcaatagc
     3961 tccttatatg atatcagttg ctcttttttt tgcagcaata tcaacaaata tgatatttgc
     4021 gaaattgcct tcaggacgtc atccagagag ccgttgggct tggttgaaat cttgagctga
     4081 aataaatggt attatagctg ttttggcagg aattttggta tatggaggag ttcagcttat
     4141 tggtttaact gctaatcatg agatgagaat atttattctc atcatcctaa caagtttagt
     4201 attcatgtct atggtgacca ctttagcaac gtggaatagc cgtataggag cttttttctc
     4261 acttattttg cttttactac agttagcatc aagtgcaggt acttatccac ttgctttgac
     4321 aaatgatttc tttagatcta ttaatccctg gttaccaatg agctattcag tttcgggatt
     4381 acgacaaaca atctctatca acaagtcatt ttcctagctg tcatactagc tctatttact
     4441 ggtttaggta tgctagccta tcaacataag aaaatggaag aagattaaaa aaatcgaccg
     4501 attaactggt cgatttttta tgccttagat gactttcgtc tgtgattata gattccaaat
     4561 agtaagagag aagtaaagga acagattgct ccagtaataa aaccattggg aatgaaggaa
     4621 agtgtaatag ttcctttccc cttgggaatg tcaactttca taaatccagt ttgagcttgt
     4681 ttaatttcta ttttcttacc atcttggtag gcagaccaac ctttgtcata aggaatggtg
     4741 aagaaaatag atgtatcttg ttggacatca tatgtagcaa aaaccttgtt tttagaagtt
     4801 gatactgtga caggttgttc tttaattttt tgaattgcct cggtgaaagt tttggtatct
     4861 aaacgataga aggtaggaga ttcaaatgat acttgtgaat ttccagggaa actaacattg
     4921 atattgaaag tttttttctc tttagtatat cctagattaa agaaggagaa gacattatca
     4981 gttgtaaaag tctttttttc accatttaca aggatgtcaa ccttcttttg tttatcgtta
     5041 gaaaagtgaa ggtttatgaa agagagataa acttggctgt tttctggaac ttcaatttga
     5101 tactggattg ctgcatcttc atttgaagaa cttgtgacac taatcaaatc attagtattt
     5161 tctatttttt ctgttttttc ataaggtatt ggagaaaaat aatcaaaatt gacattagca
     5221 agttgattta aaaatgaggc ctgattatcc aaggtatgtt cattgaactt gacatcattg
     5281 taaacagatt gactagcaac tgcaatcgga agagagtatt gattttcata tagggtaaga
     5341 ttatcttttt gatagatatc tttaaagcca tacttatcaa taggactgtc tgagatattg
     5401 tactggatac caaataaact atcagccaaa atactattat ttgcatatcg gagattgaga
     5461 ttagtcccag aggatttaaa accaagttta tctaaagtag agcttgatga acgatttcga
     5521 acagatgaaa attgagagat tccattgtag ttgaatttca tactgtcatt tcctgtctga
     5581 gtttgtagtt tttcagtacg agtaaattga tttccaatat atgttgagaa agattccata
     5641 gctgggatat ctcgactata agcacttcga gaagcaaatc cccattcctt agcaattccg
     5701 tccatttgag atgaagcatt taaactcatt tcaaccagta taaataaaga gattagaatg
     5761 gcaaatagat tcacagatat aaactttttg ataactgcaa ggagtaaaag agaatagaca
     5821 accaaaaatt taagagtaag cagaatattc aaatctgtta aaaaagaata atgcgatttt
     5881 agatagatgg tagctaaaaa tcctgttact acaagaaaaa gcgaaactaa aaaattccag
     5941 actttaagtt ctttcagacg ctttaagact tctgctgctg tgtaaattaa caaggtagag
     6001 aaaatccaag catagcgatg taaaaacatg tttggagtat gcatgccttg ccaaaataag
     6061 tcaagagctt ctatgtaaaa gcttgcaatt agaaatgtaa agaatattac atatatgagt
     6121 ttcacgtgaa acttaataga tttcagcgta aaaaataaaa tggtcaaaat aaagggaagt
     6181 agtccaacaa aaatcattgg gatggcccca tactttgttg tgtcaaagga accaatgaat
     6241 tgcttagcaa agagatcaag ataccagcta ctttcagttt gaaactttgt aacttcagtc
     6301 aatttttccc catgtgtctg taaatcaaac agagtgggaa gagtcataat caaactagcc
     6361 ataccagcta aaaaggagat aactatgaaa tcaagaacag atgattttcg agtcttaaag
     6421 tcccacgaaa tttgacagag ataccagaaa ataagaaaca atactgtcat atatccaaaa
     6481 taataatttt gaataaataa gattgacaga cttgtaaagt acaataggag tttcttttca
     6541 gttatcagta gatgtaaacc agttataatt aaaggaatca agataaaaac atctagccag
     6601 gtttttatct ctaattgact gacagtgaaa ctcatcagag cataggaagt agataaggct
     6661 agttttaaag tctgagggat agattgaaac aatttattca aactaaaaaa ggttgacaga
     6721 ccaatcaatc caaattttaa gagagttgtc agatagatag catctggcat attcgttaga
     6781 tcaaaaaagt aaaccagagg cgcgagaaaa ctacccaagt aataactaga tagggcatag
     6841 aagtttagcc ctagaccact tgtaaaggtg taaaacagac tactatttcc atgtaggata
     6901 tttcgtaagg ctacatcaaa aataacgtat tgatgaaagc catctcctaa tagaggagag
     6961 ttgtcgctat tccagtagat accttgagat agatatactc cagacataat cactatagga
     7021 atgatgaaag aaataaaata ggttcgatat gtttttaaaa atgatttcat gttacctcgt
     7081 agaatgatag aaaactcagt tggttaaccc aactgagttt tgaagtttta tttagtcttt
     7141 ccaaagttct ttaacttttg cttgtacttc tgcattttct aggaattcat cgtaggtttc
     7201 atcgatacgg tcaatgacgc catttttaga taagacaatg atatggttag ccaaagtttg
     7261 aataaattcg tggtcatggc tggcaaagat gattgattct ttaaagtttt tcaatccatc
     7321 attcaagctt gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa gtacaaggac
     7381 atttgatttt aagagcatga gttttgaaag catgacacga actttttctc cccctgacaa
     7441 gacatttaca ggtttgttaa cttcatctcc agagaagagc atacggccga ggaagccacg
     7501 taggaaagta ttgtcatctt cttctttact tgcgaattga cgcaaccagt caagaattga
     7561 ttctcctcct gcaaaatcag ctgagttatc ttttggtagg taagattgac tagttgtaac
     7621 tccccacttg acagttcctt catagtcaat atctcccatg attgcacgaa ttaatgcagt
     7681 cgtttgaata tcattttgtc caataagtgc tgtcttatca tctggacgca agatgaaact
     7741 aatattatcc aagatagttt caccatcaat ctttacagtt agattttcta ctgtcaagag
     7801 atcattacca atctcacgtt ccgctttaaa gttgataaat ggatatttac gactagatgg
     7861 cacaatctct tctagctcaa tcttatcaag cattttctta cgtgatgttg cctgccttga
     7921 cttagaagca ttggcagaga aacgagcaac aaattcttgc aattgtttaa ttttttcttc
     7981 tgctttagca ttacggtctg ctagcaattt agcagcaagc tcagaagatt ccttccagaa
     8041 gtcgtagttt ccgacataga gtttgatttt tccaaagtca aggtcggcca tgtgagtaca
     8101 aactttgttt aagaagtgac ggtcgtggga tactacgata actgtgttat caaagtcaat
     8161 taagaagtct tctaaccaag taatcgattg gatatccaaa ccgttagtag gctcgtccaa
     8221 gagaagaaca tctggtttac caaaaagtgc tttggcgagg agaaccttta ctttttcacc
     8281 gttggccaat tcgctcatgt tttggtagtg taattcttct ggaatgttta ggttttgaag
     8341 tagttgagag gcttcactct ctgcttccca acctccaagt tcggcaaact ctccttcgag
     8401 ttcggcagca cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga tagcatcttt
     8461 ctctttcatg atgctataaa gtttttcatt tcccatgata acgacatcaa tggcacgttc
     8521 atcttcgtag tcaaagtgat tttgacgaag aacagagaga cgttcatctg gaccaagaga
     8581 gatgtgacca gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg ttgattttcc
     8641 ggcaccatta gcaccgatta atccgtaagt atttccttct gtaaatttga tattgacatc
     8701 atcaaaaagt ttgcgatcac taaaacgtag tgaaacatca gatactgtaa gcaatatttt
     8761 tctcctatat gt
//
LOCUS       DAVKAO010000072         8647 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKAO010000072 DAVKAO010000000
VERSION     DAVKAO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.072"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            13..1014
                     /locus_tag="VWX16_001726"
     CDS             13..1014
                     /locus_tag="VWX16_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9159035.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1619..1696
                     /locus_tag="VWX16_001727"
                     /pseudo
     CDS             1619..1696
                     /locus_tag="VWX16_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2197..3558
                     /gene="trpE"
                     /locus_tag="VWX16_001728"
     CDS             2197..3558
                     /gene="trpE"
                     /locus_tag="VWX16_001728"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9159036.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVIHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3555..4121
                     /locus_tag="VWX16_001729"
     CDS             3555..4121
                     /locus_tag="VWX16_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9159037.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            4132..5136
                     /gene="trpD"
                     /locus_tag="VWX16_001730"
     CDS             4132..5136
                     /gene="trpD"
                     /locus_tag="VWX16_001730"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9159038.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5133..5900
                     /gene="trpC"
                     /locus_tag="VWX16_001731"
     CDS             5133..5900
                     /gene="trpC"
                     /locus_tag="VWX16_001731"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9159039.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5887..6486
                     /locus_tag="VWX16_001732"
     CDS             5887..6486
                     /locus_tag="VWX16_001732"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9159040.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6464..7687
                     /gene="trpB"
                     /locus_tag="VWX16_001733"
     CDS             6464..7687
                     /gene="trpB"
                     /locus_tag="VWX16_001733"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9159041.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7680..8456
                     /locus_tag="VWX16_001734"
     CDS             7680..8456
                     /locus_tag="VWX16_001734"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9159042.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     NLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8428..8529)
                     /locus_tag="VWX16_001735"
                     /pseudo
     CDS             complement(8428..8529)
                     /locus_tag="VWX16_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
BASE COUNT         2568 a         1616 c         2069 g         2394 t
ORIGIN      
        1 tggaggactg aaatgaaaaa tataaatggg aaaaaggtaa ctatttatga tattgcacgt
       61 ttatcaggtt tttctccaaa aacagtttca cgagttatta atggtggagt aaatgttaaa
      121 gaagaaactt atcaagcgat tcaaaaagtt atagaggaat tgtcgtatat tccaaatgcg
      181 tatgcaaaaa atttaactaa aaaagaagct ataaatatat tgatttctgt aaaaaaaatc
      241 gactcttttc ctttaatttg gtttcatacg ctgttagata aagtgttacg gacgtgtaaa
      301 gaatttggtg ttaatgctat agttgagtac tttggagagg aggatacaat tagtaattca
      361 attatatcaa gcacaggtag cctagtagat ggtgttattg ttttttatga aagtgtagat
      421 gatatcagaa ttcagtattt aaagaagaac catatgcctt ttcttgtttt cggtgaatct
      481 caaacgtctg gagtagtcta tgtatctaat aataattttc aagctactta tgatatgatg
      541 aaagctgtaa ctgaagaaaa atttaaaaat atgtggttgc ttatgggagg agaatcccat
      601 gttaataagg atcgtgaaag aggtgtgcgt tcttttctga atgataaaaa ttactttatg
      661 gatttaaaag tcatttatgg cttatctaca attgattcag tttattctta tgcaatgcag
      721 cacttgacta cccaaaatta tccggacatt atttttgttt caggagatga aaaggttcag
      781 ggcttgattc gtgcgtgcta tgagaaagga attcttattc cagatgatat ttctatcatt
      841 ggatttgata atattcctat ttctcagtat tatactccag ctttgtctac aattgctccc
      901 aattatgtta aattggctaa agaaatgata gaaggtgttt tagcaattat taaaggggaa
      961 agtgtcacat ctgttgaagt ttctcctaaa tttgttagga gacagagttt ctagtattgt
     1021 ttaattcttg aaaatcctag tggaaaagat ggagtgttct aaaataagtt ctgtttagcc
     1081 tccattcagt ttctattgag ttggatccaa tttctaacaa gtgggacatg ttcatgttcg
     1141 atctaaggaa gatatggtca cattcgaatg atatcaaaaa aatcgtaaaa aagtcttgac
     1201 aaagaaaaaa caaagtatta aaataagttc aacaaatcag aaaagtaact atttttgatc
     1261 ttcagggagc ctgtggtgat tgtgaatagg tggttggaag tagtgaaagt gggctgattt
     1321 tgaaaatgaa tttgaaacaa tgaaaattcg gtgcgcacac cttacagtgc agcttgttgt
     1381 taaacaaggc agagatgtaa agggatagtc cctttataat tgaggtggca ccgcgttacc
     1441 aacgccctca cacggaagta tattctgtgt gtgggctttt ttctatccgt cgtttggttt
     1501 atcttttatt agggccttaa gtcgctttga tgaacttgag ttctatctac agctccttgc
     1561 ctcctactaa aagtaaacaa aaaggctgga ctaatacgaa aaaagctagt aatactcaat
     1621 gaaaatcaaa gagcaaacta ggaaactagc cgcaggttgc tcaaaacact gttttgaggt
     1681 tgtggataga actgacgaag tcagctcaaa acactgtttt gaggttgcag atagaactga
     1741 cgaagtcagt aaccatacct acggcaaggc gacgttgacg cggtttgaag agattttcga
     1801 agagtataaa ggtgtaccga gatgaacatc tcttcatcaa tgtctctggt cgtggaggca
     1861 aggacctagc tgcgattgca gattactaga agctaaaaaa taatagatgg aaaaattaca
     1921 gtcttttctc tcacagagag cttgtggttg ctgaaaacaa gcagagaaag ctgtaaagtt
     1981 gggttcatct gaaacgagag aagaaaacat cattctcaag ggagtgccct ttatcgcaca
     2041 gcctgttaca ggagatatct gagacaggaa tgagagatag gagaaatcct ataattgagg
     2101 tggcaccgcg aatttcgtcc tcacgcaagt tattttgcgt ggggattttt catacagtcg
     2161 ccacgaacta gaattagaac tgaaagggaa atgacaatgg aacgaatcat tcatggagat
     2221 gtcttatcac caatcttggc ttatatgcgc ttaaaggggc aacataaggt tattttagag
     2281 agtattccga gagacaagga aacagctcgt ttttctatcc tagcctataa tccagttttt
     2341 gagattaagt ttgaaaatgg agttctctat caaaatggtc aagcgattga tcgggatccc
     2401 ttggatttcc tttatgaagt gattcataag agccagcacc attcagacct cccttttggt
     2461 gggggagcca ttggttttgt tggttacgat atgatttcgc tttatgaaga aattggtcaa
     2521 atccctgagg atacaattgg gacgccagac atgcatttct ttgtctatga gagctatatg
     2581 gtctttgacc acaagaagga gaagatacat gtcatagagg atgcacttta tagtgagcgc
     2641 agtcaagagg ccttggaaaa atccttgaac caagtgcttg aggaattacg cattcctgct
     2701 ccaaatgaat ttgaagactt ggatttatca ccacttgact tcaaaccgca tatcgctcca
     2761 cataagtttg agggaatggt agaaacagct cgtgacttga ttcgtaacgg agatatgttc
     2821 caatgtgtac tcagccagcg tttctcagca gaagttactg gaaatccatt tgacttctac
     2881 agaaatctcc gcgtgactaa tccatctaat tacctttatt tctatgattt tggggattat
     2941 caaatcatcg gagctagtcc agaaagtttg gtttctgtca aaaatggcat cgtgacaacc
     3001 aatccgattg cagggacgcg accaagaggg gctacggatg aagaagacaa ggccttggcg
     3061 actgacctcc tctctgatga gaaggaaaca gcagaacatc gaatgttggt agacttgggg
     3121 cgtaacgata ttggccgcat ctctgaaacg gccagtgtcc aagttactaa gtatatggag
     3181 gtggagctct tccgctacgt catgcatttg accagtgtgg tcaagggacg tttgcttgca
     3241 gaactcactg ccatggatgc cttgaaagca acgcttcctg ctggaaccgt ttctggagca
     3301 ccaaaaattc gagctatgag acgcatctat gaactggaaa cggaaaaacg gagcgtatat
     3361 gcaggagcaa tcggctactt gtctgcgacg ggtgatatgg atttggccat tgccatccga
     3421 accatgattc tcaaaaatca aagagcctat gtgcaggctg gggcagggat tgtctacgac
     3481 tctatcgccc aaaacgaata ccaagaaacc attaacaaag caaaatctat gactagaatt
     3541 ggagaactaa gaccatgatt ttattgattg acaactatga ttcttttacc tataacttgg
     3601 cccaatacat tgggaatttt gcagaagttc aggttctgag aaatgatgat tccaagctgt
     3661 atgaagaagc tgaaaaagca gatggtctgg tcttttctcc tggtcctggt tggccagttg
     3721 atgctggaaa gatggaagac atgattcgtg attttgcagg caagaagccg attcttggga
     3781 tttgtttggg ccaccaagcc attgcagaag tctttggtgg taagctaggt ttggctccaa
     3841 aagtcatgca tgggaaacag agcaatatca actttgaagc gccatctgtt ttgtatcaag
     3901 gtattgagga tggccgtgcg gtcatgcgtt atcacagtat tttgattgag gaaatgccag
     3961 aagactttga agtgacagct cgttcgactg atgaccaagc catcatgggg attcaacata
     4021 aaaacctacc gatttatggc ttccagtacc atccagagag cattggaacg ccagacggct
     4081 tgtcttctat tcggaatttt atcgaggagg ttgtaaagtg aggaaactag gatgaaagag
     4141 attattgaaa aactagcaaa atttgaaaat ttatcaggtg tggaaatgac ggatgtcatt
     4201 gagcgtatcg taactgggcg tgtaactgaa gcgcagattg cttctctcct cttagctctt
     4261 aagatgaagg gggaaacacc tgaagaacgc acagccattg cccaagtcat gagaggacat
     4321 gcccagcata ttccaactga gattcatgat gctatggaca actgtggtac aggtggggac
     4381 aagtctttca gttttaatat ttccacaact gcagcctttg tcttggctgg tggcggtatt
     4441 cacatggcca agcatggtaa ccgctcgatt tcttctaaat ctggttccgc agatgtcctc
     4501 gaagccttgg gaatcaatct tgacctcaaa ccagctgaac taggtaaggt ctttgataaa
     4561 actggaatcg tctttctctt cgctaaaaat atgcacccag ctatgaaata catcatgcca
     4621 gctcgtttgg aattgggaat tccaacgatt atgaacttga ctggtccact gattcatcca
     4681 atggctttag aaacacagct tcttggaatt agtcgtccag aactcctaga aagtacagct
     4741 caggttttga aaaatatggg tcgcaaacgt gccatcgtgg ttgctggacc agaagggttg
     4801 gatgaagctg gcttgaacgg aacaaccaag attgcacttc ttgaaaatgg cgaaatcagc
     4861 ttgtcaagct ttactccaga ggatttggga atggaaggct atgctatgga agatattcgt
     4921 ggtgggaatg ctcaggaaaa tgcagaaatt ttgcttagcg ttctgaaaaa cgaagcaagt
     4981 ccattcttgg aaacgacagt cttgaatgct ggtcttggtt tctatgctaa tggtaagatt
     5041 gatagcatca aggaaggagt tgccttggcc cgtcaagtga ttgctagagg caaggccctt
     5101 gaaaaactca gactgttaca ggagtaccaa aaatgagtca ggaattttta gcacgaatct
     5161 tagagcagaa ggcgcgtgag gtggagcaga tgaagctgga gcaaatccag cctctgcgcc
     5221 agacctatcg cttggcagaa tttttgaaga atcatcagga ccgcttgcag gtaatcgctg
     5281 aggtcaagaa agctagccct agtttgggag atatcaatct cgatgtggat attgtgcaac
     5341 aggcccagac ttatgaagaa aacggagcag tgatgatttc ggtgttgaca gatgaggttt
     5401 tctttaaagg gcatttggat tatctacggg aaatttccag tcaggtagag attccgacgc
     5461 tcaacaaaga ctttatcata gatgaaaagc aaatcatccg cgctcgcaat gcaggtgcga
     5521 cagttatctt gcttattgtg gcagccttgt ccgaagaacg cctcaaggaa ctgtatgact
     5581 acgcgacaga gcttggtctg gaagtcttag tggagactca caatctagct gaactagagg
     5641 tagcccacag acttggtgct gagattatcg gggtcaacaa ccgcaacttg actacctttg
     5701 aggtcgactt gcagaccagt gtagatttag ccccttactt tgaggaaggt cgctattaca
     5761 tttctgaatc tgccattttc acagggcagg atgcggaacg actagcccca tactttaacg
     5821 gaattttggt agggacagct cttatgcagg cagaaaatgt ggcccagaga atcaaggagt
     5881 tgcagattga caaaggttaa aatttgtgga ctatcgacca aagaagcggt ggaaacagcc
     5941 gtttcagcag gagccgacta tatcggtttt gtctttgcac ctagtaaaag acaggtgact
     6001 ttagaagagg cagctgagtt ggcaaagctt attcctgcag atgtgaaaaa ggttggagta
     6061 tttgtttcac caagtcgggt agaactgctg gaagcgattg acaaagttgg cttggacttg
     6121 gttcaagttc acggtcaggt ggcagatgat ttatttgaga atttgccttg tgccagtatt
     6181 caggctgtgc aggtagatgg aaatgggcat gtccccaatt ctcaggcaga ttatctactc
     6241 tttgatgccc ctgtggcagg aagtggccag tcctttgatt ggggtcaact ggatacgact
     6301 ggactagcac agcccttctt tatcgcaggt ggccttaatg aagataatgt agtaaaagca
     6361 attcaacatt ttactcccta tgcagtagat gtatcgagcg gagtggagac agatggacaa
     6421 aaagatcatg aaaagattag aagatttata gagagggtaa agcatggcat atcaggaacc
     6481 aaataaagat ggattttacg gaaaattcgg cggacgtttt gtcccagaaa cattgatgac
     6541 agcagttttg gagttggaga aggcctaccg tgaaagtcag gcagacccaa gtttccaaga
     6601 ggaattaaac caactcttgc gccagtacgt aggacgtgaa actcctcttt actacgcaaa
     6661 aaacttgacc cagcatatcg gcggagccaa gatttatctc aaacgggaag accttaacca
     6721 tacaggagcc cacaagatta acaatgcctt aggacaagtt tggcttgcca aacgcatggg
     6781 taaaaagaaa attatcgcag aaacgggtgc tggtcagcac ggtgtggcaa ctgcaactgc
     6841 tgcggccctc tttaacatgg aatgtaccat ctacatgggt gaggaagatg tcaaacgcca
     6901 agccctcaat gtgttccgta tggagctttt gggagctaag gttgaggcag tgacagatgg
     6961 ttcgcgcgtg ctcaaggatg cggtcaatgc agcccttcgt tcatgggtgg ctaatatcga
     7021 cgatacccac tatatccttg gttctgcctt ggggcctcat ccatttccag aaatcgttcg
     7081 tgacttccaa agtgtcatcg gtcgcgaagc taaacaacag tatcgcgata tgacaggtca
     7141 aaatctgccc gatgccctag tagcctgtgt tggtggtgga tctaatgcta ttgggctctt
     7201 ccatcccttt gtagaagatg agtctgtagc catgtatgga gctgaagcgg ctggacttgg
     7261 tgtggatacg gagcaccacg cagctacctt gaccaagggt cgtccaggtg tccttcacgg
     7321 ttccctcatg gatgtgctcc aagatgccca tggtcaaatt cttgaagcct tctctatctc
     7381 agcaggtttg gactatcctg gtatcggtcc agagcattct cactaccacg atattaaacg
     7441 tgccagctat gtccctgtga cagacgaaga agccttggaa ggattccaac tcttgtctcg
     7501 tgtggaaggg attatcccag ctttggaatc tagccatgct atcgcttttg cggtgaaatt
     7561 ggccaaagaa cttggaccag aaaagtctat gattgtctgc ctatcaggtc gtggggacaa
     7621 ggatgtggtt caagtcaaag accgcttgga agcagatgca gcaaagaagg gagaagctca
     7681 tgcctaagac actaacagaa aaattgaatg ctataaaagc aactggaaaa ggaattttcg
     7741 ttccttatat catggctggt gaccacgaaa aaggtttgga cggtcttgct gaaacaattc
     7801 actttttaga agatttgggt gtctctgcta ttgaagtggg tattcccttt tcagaccctg
     7861 ttgcagatgg acctgttatt gaagaagctg gcttgcgcag tttagcccac gggacctcta
     7921 cccaggcttt agttgaaacc ttgaaaacca ttgaaacaga gattccactg gtcatcatga
     7981 cctacttcaa ccccctcttt cagtacggtg tggagaactt tgtcaaagat ctggctgata
     8041 cagctgtgaa gggcttgatt atcccagacc tgcctcatga acatgccaac tttgtagaac
     8101 catttttggc agatacagat atcgccttga ttcctttagt aagcttgacc acaggaattg
     8161 agcgccagaa agagttgatt gaaggggcgg agggattcgt ctatgccgtt gccatcaatg
     8221 gggtaacagg gaaatcaggc aattaccgtg cagatttgga caagcacttg gcgcaacttc
     8281 atcaagtggc cgacatccca gtcttgacag gttttggtgt atctagtcaa gccaatctgg
     8341 aacgcttcaa tgcggtgtca gatggtgtta tcgtcggttc gaaaatagta aaagctctcc
     8401 accaaggaga gccgattcag gactttatca aacaagcagt agcttaccaa aaataatcaa
     8461 acaagtagca agtaagagga aaggaacgaa aggaagtctt tcctttttct tttgcaggat
     8521 aaaggtcagt tttatagtaa attgaatctg gaatattaca ctacggttcc taaaatattt
     8581 tcagaaagtg atttgaattt cccaataaat ttgttcagtt tttaattgat tttactatag
     8641 aagatat
//
LOCUS       DAVKAO010000073         8372 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKAO010000073 DAVKAO010000000
VERSION     DAVKAO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.073"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            59..1120
                     /gene="dinB"
                     /locus_tag="VWX16_001736"
     CDS             59..1120
                     /gene="dinB"
                     /locus_tag="VWX16_001736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9159043.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSIAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(1267..2112)
                     /locus_tag="VWX16_001737"
     CDS             complement(1267..2112)
                     /locus_tag="VWX16_001737"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9159044.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLVATLPLLGV
                     FKFDDWFDTHFHNMVSVAIMLIIYGIAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            2352..3317
                     /locus_tag="VWX16_001738"
     CDS             2352..3317
                     /locus_tag="VWX16_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9159045.1"
                     /translation="MWRYSHALCGIDVAKNKHDVTALNVSGKTVLKPLTFSNNKAGFE
                     LLDLSLRQLNQDYLIALEDTGHYAFNLLNFLHEQGYKVYTYNPLLIKKFAKSLLLRKT
                     KTDKKDAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAPR
                     SIGTTSPAREFEIIENIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGNRLGAVILA
                     EIQNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMVKRGSPHLR"
     gene            complement(3696..5585)
                     /locus_tag="VWX16_001739"
     CDS             complement(3696..5585)
                     /locus_tag="VWX16_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9159046.1"
                     /translation="MKKTFFLLMFGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSTAQMSHYASVANTASTYSVSSGSGSSGGVFSGGGGGG
                     SIGAF"
     gene            5724..7289
                     /locus_tag="VWX16_001740"
     CDS             5724..7289
                     /locus_tag="VWX16_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9159047.1"
                     /translation="MRKIYLSIFTSLLLMLGLINVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAIKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLIKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGSSITLLISIVGTITGLIIGLAIGVFRTAPLSENKVIYG
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            7289..8029
                     /locus_tag="VWX16_001741"
     CDS             7289..8029
                     /locus_tag="VWX16_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9159048.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(8138..8353)
                     /locus_tag="VWX16_001742"
     CDS             complement(8138..8353)
                     /locus_tag="VWX16_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159049.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFKTALGLIDFYEKNYEKFED"
BASE COUNT         2657 a         1836 c         1551 g         2328 t
ORIGIN      
        1 gcagatagag ctgacgtggt ttgaagagat tttcgaagag tataaaaagg tgctaggcat
       61 gttgattttt cctttgttaa atgatttgtc aagaaaaatc atccatattg acatggatgc
      121 cttttttgct gcagtggaaa tcagggataa tcctaaactc agaggaaaac ctgtcattat
      181 tggaagcgac cctcggcaaa caggtggacg gggagtcgtt tctacctgta gttatgaggc
      241 aagagctttt ggtgtccatt ctgccatgag ttccaaggaa gcttatgaac gttgtcccca
      301 ggctgtcttt atctcaggga attatgagaa atacaagtct gtgggactcc agattcgagc
      361 tatttttaag cgctatacag atttgattga acccatgagt attgacgaag cctatttgga
      421 tgtgacagaa aataaactcg gtatcaagtc agcggtcaaa attgctcgcc tcattcaaaa
      481 agatatctgg caagaactcc atctaactgc ttctgccggt atttcctata acaagttctt
      541 agctaaaatg gcgagtgatt atcaaaaacc tcatggtttg acagtgattc tacctgaaca
      601 ggctgaggat tttctcaaac aaatggatat ttccaaattt catggagtag gaaaaaagac
      661 agtagaacgt cttcatcaaa tgggcgtttt tactggtgct gatttacttg aagttcctga
      721 ggtgacccta atagaccgtt ttggtagact aggctatgat ctgtatcgaa aggctcgtgg
      781 cattcacaac tctccagtca aatccaatcg catccgtaaa tcaatcggca aggagaaaac
      841 ctacgggaag attctccgtg ctgaggaaga tatcaaaaaa gagctgactc ttctatcaga
      901 aaaagtcgct ctcaatctac atcaacaaga aaaagctgga aaaattgtca ttttgaaaat
      961 ccgctacgag gacttttcaa ctcttaccaa acgaaaaagt attgctcaaa aaacacaaga
     1021 tgctagtcag ataagccaaa tagccctgca actctatgaa gaattaagtg agaaagaaag
     1081 aggtgtccgc ctattgggga ttaccatgac tggattttaa agctcaatga aaatcaaaga
     1141 gcaaactagg aggctagccg caggctgctc aaaacactgt tttgaggttg cagataaagc
     1201 tgacgcggtt tgaagagatt ttctaagagt ataaaacctt gaagagttgc cccttcaagg
     1261 tttttcttat acaaataaac ggacaaaact gtaaagtagc aaaacactac caagcacgat
     1321 acggtattta ccaaaaaggg tgaagtcgtg ttttttcaca tagctggtca agaagcgaat
     1381 agccaccatg ctgaccgcaa aagctactcc catcgcgacc aagagcaaaa acaattgccc
     1441 aaagctcaag agttctccgg ctttcacaaa tttgaaaatc tttaaggcac tagctccaaa
     1501 cataacagga atcccaagat agaaggtaaa ttctgtcaca actgaacgac tggttccatt
     1561 taacaaacca ccgacaatcg ttgcacctga acggctagtc cctggtaaaa gagcaagaac
     1621 ttggaagagt ccaatataga aagcggtcgt ataaggaagc ttgtccaact ctgttacact
     1681 tggctcgata gcacgcgctt tattgcgctt ttccaaatag atgaaggcaa tcccgtagat
     1741 aatcaacatg atagcaactg aaaccatgtt atggaagtgg gtatcaaacc aatcatcaaa
     1801 tttaaagaca ccaagtaaag gcaaagtggc aaccaagacc ttcaaccaca gtctccaagt
     1861 cttacgaact tcctgtttgt ccttagtcgg tttaaaagga ttgagcttgt taaaataaat
     1921 caccataact gctaaaatag caccaagctg aatcacgaca ttaaacatgg acataaaggc
     1981 ttcattttga ttttggtatt ggataaactc ctctgctaaa atcaaatgac ctgtactgga
     2041 aatcggcaac cattccgtaa ttccttcaac aatcccgaag aagatagatt ttaaaatttc
     2101 aataagatac atagatttct ccttatatta tatttagcgc agttagctac tgccaaagcc
     2161 caagtggtat acttggaata agccactgtg gattagttca tttcctccaa tcgcttgacg
     2221 atttccgaaa ggatctaacc acagcttttt gtttcaattg gtaatgagtt tgataacgca
     2281 tagacgtttt atctcgtcta aggtaacaag gcaaaaagtt cgtggataac caaatagact
     2341 tgaaatacta tatctggagg tactcccatg ccttatgtgg cattgatgtc gctaaaaata
     2401 aacacgatgt tacagccttg aatgtttcag gaaaaactgt tcttaaacca ctcacttttt
     2461 caaataataa agctggtttt gaacttttag atctgtctct tcgacagctc aaccaagact
     2521 atctcatcgc tcttgaagat accggacatt atgcctttaa tctactgaac ttcctgcatg
     2581 aacaaggata taaagtttac acctacaatc ctttgctcat caagaaattt gccaaatcat
     2641 tattacttcg caagactaaa acggataaga aagacgctca tggcatcgct cttaaacttc
     2701 tttctgaccc caatcgtgaa caatttcaac acgataatcg gcaagtagaa ctaaaaatac
     2761 tggctagaca tattcatcgt ctcaagaaaa aacagtctga ttggaaagta caatacactc
     2821 gttgtcttga tatcatcttt cctgagttgg ataaaatcgt tggaaagcat tcagaatata
     2881 cctaccaact cttgacatgc taccctaatc ctcagaaaag gcttgaggca ggatttgata
     2941 agctgataga aattaagcga ttgaccgctt ctaaaattca ggatatcctc tcagtcgcac
     3001 ctcgttctat cggaacaaca tctcctgctc gtgaattcga aatcatcgaa aacatcaaac
     3061 attacaagag gctcattgac aaggcggaaa catgtgtcaa tgacttgatg gctgagttca
     3121 actcggtcat cacgacggtt actgggattg ggaatcgttt aggggcggtc attttagccg
     3181 agattcaaaa tattcatgcc tttgataatc ctgctcaatt acaagctttc gctggactgg
     3241 attcttctat ttatcagtca ggtcagattg atttagctgg aagaatggtc aaacggggtt
     3301 cccctcatct gcggtaggta ctcatacaag ctgccaaagc atgccctcgc ttttcacctg
     3361 cttttaagac ctatcttaag actaagttag aacaaggaaa acattacaat gtagccatca
     3421 tccaccttgc aaaaaaactt atccgaaccc tgttttatat cctaaaaaag agctgccatt
     3481 tgacgaacaa aaagtgatgt aatttctgaa attaacacag gattaccttt gaaaaaatgg
     3541 actttttcaa gggctttttg ccgtgctcta aaatcatcgc caaatcatca aattgaaata
     3601 agaaatagtt tagataaaac tattgacttt tcttatagtt tgtctttttt ctatcttcca
     3661 ttatatcata ctttttcagt ctatggtagc tctctttaaa aggcaccgat actgccgcca
     3721 cctccgcctc cagagaagac accaccagaa cttccacttc cagaagatac agagtaggtg
     3781 cttgctgtat ttgcgacact agcataatgg ctcatttgtg ctgttgaatg ataaaacata
     3841 ctgtgccagc cataagctac atagagattg atatctggat tttccacttg aatctgatga
     3901 accttcatca aatgactaac cttgtccgca tagccaaata aggttgcata gaccaagaga
     3961 cgattccaaa caacaatact ttccaattca gccttatcca gtcgtgcaat ctcacgtaac
     4021 atattttcaa aactggtcca gagatagtag acctcagctc ccgcttcatt tagaacacca
     4081 tcacgattat ctagtcgaag cttccaataa tagaaaacag acaaaactaa ccctagaaaa
     4141 ccaagtattg gcaaagggag gtaaagatag ccatgaacgt ctaaactgta caagaacaaa
     4201 ccaaatccga taaatagggg caagatagtc aagacaccca tacccacttg caaagtcttt
     4261 tccaaaccag ttaaaggacg atagtaatct gggagccccc agaaggaaac tcgttttctc
     4321 actccttctt gcatctggtt caatacctct tcaaaagaag atttgagttg aagccctctt
     4381 gcttgaatcc gtttttcatc agaaactttg gctctacgat aaagactatc agataccttg
     4441 taatccgcaa acaaattgga aagagtttct tcttttttac ctgaaaaagc tagatttagg
     4501 cagtctttct caaagcttga caaaccatct tcttttacta gcctcaaacc aactgcatct
     4561 ccttctgaaa tgatagagac attcccacgg tctatcacat ctagcaaggt agcttgaata
     4621 agttgatcaa aggtgaattt tccagctccc ttgaccaagg gactcacttc ctccaaggag
     4681 gtcgagtaga ctgcttctga taaaaccata ggctctaatt ccattggtgg ttcatagaga
     4741 cgatgatttt tggcatattt gactgaagga gtggtctttc ttctataaat aaaatagaag
     4801 cagacactca ataacaagga gatagaaagt atcgaaggga ggacccaagt aacgagttgt
     4861 ttactctgat ctttttctct aacaatcgag tcttctatct tattaaactc ttctaaacga
     4921 ttccctttca atccctgatc cctagcgcta gcaaaatcgg tccgaggcca ataggcatgc
     4981 aactcaactc cacgcttagc cggaagattg tctaaacgga tagtataatc aaggttactc
     5041 ttttcaatcg ttccctctct aaaaagtttc cctgtatgga aaaagagttt ttcagccccc
     5101 ttgtctcccc ttacatgaaa ttcaaacttt tcaatagact ctgaactatc tgtcagaggt
     5161 tgccaattta attcagcgat atcatcataa aggaaaagta aattttttaa gttccagacg
     5221 aggtcaactt caactatgtc gccctcctga cctggattat agactctcac agtataacca
     5281 tccgcttctt ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt tttcgcggcc
     5341 tgaacctttg gatgagggtc aatgtcaaac ccgctaggca tcttaccagc acgtccaagt
     5401 cccacgattt gtcccttaaa gtcctcctca aactgataaa ctatcttttg tctaaattct
     5461 gccgtattgt ctgcatgaat atacaaatca ccttgataag agtttatctt aaaatcaatg
     5521 gcgaaaacag aaagtggcag aaggcaaaac aagccaaaca tcagtaagaa aaaagttttt
     5581 ttcatcaatt aagccccctt tttatctttc ttatcattat agcatttttt cgtaagtaaa
     5641 ggcttactta tgttgaaaaa ttacgcattt ttcgataaaa taggagacag ttatttttta
     5701 atagattaga aataggagaa atcatgagaa aaatatactt atctattttc acaagtctct
     5761 tgctgatgct aggacttatc aatgttgctc aagccgatga atatttacgc atcgggatgg
     5821 aagcagcata tgctcccttt aactggaccc aagatgatga tagcaatgga gctatcaaaa
     5881 tcgatggaac caaccagtac gccaatggat acgatgtcca aattgccaag aaaatcgcta
     5941 aggacttagg taaagaacct ttggttgtta aaaccaagtg ggaaggtcta gtccctgccc
     6001 ttacttctgg taagattgac atgattatcg caggtatgag tcctactgcc gaacgcaaac
     6061 aagaaattgc cttttcaagc agttactata ctagcgaacc agttttgctt atcaaaaaag
     6121 attctgccta cgcaagtgct aaatctttgg atgactttaa cggtgcaaaa atcacttctc
     6181 aacaaggggt ctacctctac gacttgatcg cacaaatccc aggtgctaaa aaagaaacag
     6241 ccatgggaga cttcgctcaa atgcgccaag ctcttgaggc tggtgtcatt gatgcttatg
     6301 tttctgaacg tccagaagca ctgactgctg aagctgcgaa ctctaagttc aagatgattc
     6361 aagtagaacc tggtttcaaa actggggaag aagatacagc tatcgctatc gggcttcgta
     6421 aaaatgacaa tcgtattagc caaatcaatg ccagcattga aaccatttca aaagatgacc
     6481 aagttgcctt gatggatcgt atgatcaagg aacaacctgc cgaagctaca acaactgaag
     6541 agactagcag tagtttcttt agccaagtcg ctaaaattct ttctgaaaac tggcaacaac
     6601 tcttgcgtgg ttctagtatc actcttttaa tctctatcgt cggaaccatc acaggtctca
     6661 ttattggact tgccattggt gttttccgta ctgctcctct ctctgaaaac aaagtcattt
     6721 acggcctaca aaaactagtc ggctggatcc tcaacgtcta cattgaaatt ttccgtggta
     6781 cgccaatgat tgttcaatcg atggttatct actatggaac tgcccaagct ttcgggatca
     6841 accttgaccg tacactggct gctatcttca tcgtttcaat caataccggt gcctacatga
     6901 ctgaaatcgt ccgtggtggt atcctagcag ttgacaaggg acaatttgaa gctgcgactg
     6961 ctcttggtat gacccataac cagaccatgc gtaagattgt cctacctcag gtagtccgta
     7021 acatcctacc tgcaactggt aatgaatttg tcatcaatat caaagataca tctgtgttga
     7081 acgttatctc tgttgtcgaa ctttatttct caggaaatac cgtggcaaca caaacctatc
     7141 aatacttcca gacatttaca atcatcgccg tgatttactt tgtcctcacc ttcaccgtaa
     7201 cacgtatcct acgctttatc gagcgcagaa tggacatgga tacctacact acaggtgcta
     7261 accaaatgca aacggaggat ttgaaataat gacacaagca atccttgaaa ttaaacacct
     7321 caaaaaatcc tatggacaaa acgaagtgct aaaagacatt tcactcactg tccacaaggg
     7381 agaggtcatc tctatcatcg gaagctctgg aagcggaaaa tcgaccttcc tacgctccat
     7441 taacctactt gaaacaccaa ctgatggaca aatcctttat catggacaaa acgtcctcga
     7501 aaaaggctat gacctcacgc aataccgtga aaagttgggg atggttttcc aatcctttaa
     7561 cctctttgaa aatctcaatg ttcttgaaaa cacaatcgtc gctcagacaa ctgtcctaaa
     7621 acgcgaacgc acagaagctg aaaagattgc caaagaaaac ctggaaaagg tcggcatggg
     7681 agaacgctac tggcaagcca aaccaaaaca actctcaggt ggtcaaaaac aacgtgtggc
     7741 catcgctcgt gccctctcca tgaatccgga cgctattctc tttgatgaac caacatcagc
     7801 tctcgatcca gaaatggttg gagaagtcct caaaatcatg caggacctgg ctcaggaagg
     7861 cttgaccatg attgtcgtaa cccatgaaat ggaatttgcc cgtgatgtct ctcaccgtgt
     7921 tatctttatg gataagggcg tgatcgctga agaaggtaaa ccagaagacc tcttcaccaa
     7981 tcctaaagaa gaccgaacaa aagagttcct tcaacgctat ctcaaataaa aagaaaaggc
     8041 tgcatcaacc gtgcagtctt tttgctgtcc tcagaatgaa aaggcagatt ctctatctct
     8101 actacttgaa ggtcaagaat ccgccgttat caaaagatta atcttcaaat ttttcataat
     8161 ttttttcata gaaatcaatt aggcctaggg ctgttttaaa tgaaatattt tttacttttg
     8221 cacgaccctg cgccagagca atgatagaca tttcacgcgc attcgtttct ttagagatac
     8281 ggtagcctgt gattttctta tcacgaaccc agccaacaac tgattctact ttttcaaagt
     8341 ttgacttagc catgtttttc tcctattttc tt
//
LOCUS       DAVKAO010000074         7883 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKAO010000074 DAVKAO010000000
VERSION     DAVKAO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.074"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            148..837
                     /gene="coaB"
                     /locus_tag="VWX16_001743"
     CDS             148..837
                     /gene="coaB"
                     /locus_tag="VWX16_001743"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9159050.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISTDQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            849..1400
                     /gene="coaC"
                     /locus_tag="VWX16_001744"
     CDS             849..1400
                     /gene="coaC"
                     /locus_tag="VWX16_001744"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9159051.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLITPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            1384..1947
                     /locus_tag="VWX16_001745"
     CDS             1384..1947
                     /locus_tag="VWX16_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9159052.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            2229..2756
                     /locus_tag="VWX16_001746"
     CDS             2229..2756
                     /locus_tag="VWX16_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9159053.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            2768..3283
                     /locus_tag="VWX16_001747"
     CDS             2768..3283
                     /locus_tag="VWX16_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9159054.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(3280..3747)
                     /locus_tag="VWX16_001748"
     CDS             complement(3280..3747)
                     /locus_tag="VWX16_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9159055.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(3821..4309)
                     /locus_tag="VWX16_001749"
     CDS             complement(3821..4309)
                     /locus_tag="VWX16_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9159056.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(4275..4838)
                     /locus_tag="VWX16_001750"
     CDS             complement(4275..4838)
                     /locus_tag="VWX16_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9159057.1"
                     /translation="MIYLRKLKREDLISLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLVLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(4848..6836)
                     /gene="uvrB"
                     /locus_tag="VWX16_001751"
     CDS             complement(4848..6836)
                     /gene="uvrB"
                     /locus_tag="VWX16_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9159058.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(6913..7866)
                     /locus_tag="VWX16_001752"
     CDS             complement(6913..7866)
                     /locus_tag="VWX16_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9159059.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         2349 a         1759 c         1386 g         2389 t
ORIGIN      
        1 cgtaaatatc tctgttttta agacttttag agtcctttct taaatttttt atggctttat
       61 agtttgaaac tataataaat cttcgttttt accaaaaatt tatcactttc attttactta
      121 ccgcttattt ttgtgtacaa tagtgctatg aaaattttag ttacatcggg cggtaccagt
      181 gaagctatcg atagcgtccg ctctatcact aaccattcta caggtcactt ggggaaaatt
      241 atcacagaga ctttgctttt tgcagggcat gaagtttgtt taattacgac aaaacgagct
      301 ctgaagccag agcctcatcc taacctaagt attcgagaaa ttaccaatac caaggacctt
      361 ctaatagaaa tgcaagaacg tgttcaggat tatcaggtct tgatccactc aatggctgtt
      421 tctgactaca ctcctgttta tatgacaggg cttgaggaag ttcaggctag ctccaatcta
      481 aaagaatttt taagcaagca gaatcatcag gccaagattt cttcaactga tgaggttcag
      541 gttttgttcc ttaaaaagac acccaaaatc atatccctag tcaaggaatg gaatcctact
      601 attcatctga ttggtttcaa actgctggtt gatgttaccg aagatcatct ggttgacatt
      661 gcacgaaaaa gtcttatcaa gaatcaagca gatttaatca tcgcgaatga cctgactcaa
      721 atttcaacag atcagcaccg agctatattt gttgagaaaa atcagcttca aacagtccag
      781 actaaagaag aaattgcaga actcctcctt gaaaaaattc aagcctatca ttcttagaaa
      841 ggaaaactat ggcaaacatt ctcttggctg taacgggttc aatcgcctct tataagtcgg
      901 cagatttagt cagttctcta aaaaaacaag gccatcaagt cactgtctta atgactcagg
      961 ctgctacaga gtttatccaa cctttgacac tacaggtact ctcacagaat cctgtccact
     1021 tggatgtcat gaaggaaccc tatcctgatc aggtcaatca tatcgaactt ggaaaaaaag
     1081 cagatttatt tatcgtggta cctgcaactg ctaacactat tgcaaaacta gctcacggat
     1141 ttgcggacaa catggtaacc agtacagctc tagccctacc aagtcatatt cccaaactaa
     1201 taactcctgc tatgaataca aaaatgtatg accatccagt aactcagaat aatctgaaaa
     1261 cattagaaac ctacggctat cagctgattg ctcctaagga atccctacta gcttgtggag
     1321 accacggacg aggagcttta gctgacctca caattatttt agaaagaata aaggaaacta
     1381 tcgatgaaaa aacgctctaa tattgcaccc attgctatct tttttgctac catgctcgtg
     1441 atacactttc tgagctcact tatctttaac ctttttccat ttccaatcaa accgaccatt
     1501 gttcatattc ctgtcattat tgccagcatt atttatggtc cacgagttgg ggttacactt
     1561 ggatttttga tgggattact tagcttgacg gttaacacga ttacgattct accgacaagc
     1621 tacctcttct ctcccttcgt accaaacgga aacatctact cagctatcat tgccattgtc
     1681 ccacgtattt tgattggttt aactccttac ttagtctata aactgatgaa aaacaagact
     1741 ggtctgattt tagctggggc ccttggttcc ttgacaaata ctatctttgt ccttggagga
     1801 atcttcttcc tatttggaaa tgtttataat ggaaatatcc aacttcttct ggcaaccgtt
     1861 atctcaacaa attcaattgc tgaattggtc atttctgcaa ttctaaccct agccattgtt
     1921 ccacgactac aaaccttgaa aaaataaaaa caatctccgc tttcaaacct gaaggtggag
     1981 attttgctct atacagtttc tttttaatga aatctttact atagtaaaac gaaataagaa
     2041 caggacaaat cgatcaggac agccaaatcg atttctaaca atgttttaga agtagaggtg
     2101 gactattcta gtttcaatct actataactt ttaactaaaa aatgatctat aaacccaagt
     2161 aaattcttgc tttattgtct ggtttattgt accatactag tgtatatgca gttaaaaagg
     2221 agattcttgt gaatacacgg aaaaagacac aatttatgac aatgacagcc cttttaacgg
     2281 ctattgcgat tttgattcca attgttatgc ctttcaagat tgtcattcca cctgcttcct
     2341 atactttggg gagccacatc gctattttta tagccatgtt cttgtcgccc ttgatggcag
     2401 tttttgtcat cctagcctct agttttggat ttttgatggc tggctatccc atggttatcg
     2461 tttttcgggc tttttcccat atatcttttg gtgctttagg agctctttac ctacaaaaat
     2521 tccccgatac cctagataaa ccaaaatctt cctgggtttt caactttgtt ttggctgttg
     2581 ttcatgccct tgctgaagta ttagcctgtg tcctttttta cgcaacttcg ggtaccaatg
     2641 tagaaaacat gttctatgtt ctatttgtac tagttggatt tggcacaatt atccatagta
     2701 tggtagacta tacattagca ctagctgtct ataaagtgct tcgaaaacgc cgttaaaagg
     2761 aaaagctatg acaaaagatc gcaaacaagc cctgctccaa ctcttgaaag aagctcctaa
     2821 agcccttaat ggccaaagtt tggcggaaca ttttcatgtc acacgtcagg tcattgtgca
     2881 ggacattgca attttgagag ccgatggcgc tcctatccta tccaccaatc gtggctacat
     2941 ctataagcaa attgatacca atccttatgt tcacaaactt ttcaaagtga aacatgaagt
     3001 tgaagaaatc ggtcaggaac tccttgctat tgtcgataat ggcgggcatg tccagaatac
     3061 cttgattgac catcccgttt atggagaaat tgaaaccttg cttaaactgt cttgtcgcag
     3121 agatgtgcaa cattttctag aacaagtcga gcattctgac tttagacctc tatctgaatt
     3181 gacagatggc atccattacc acctagtcga agccgaaaca caacaagacc tccactatat
     3241 cgaggaggcc ttggatcagc taggttattt agtaaaagac tagaagattt tcttttccca
     3301 atcgtcttct gtacggcgag gatagaaaaa ctctgacttg tcgggagctt ccatcatttc
     3361 catcaatggt agcatatcat aggccagatt taagtttgga atctggtctt tttgcaccca
     3421 agaaacttct ccctcttctg aagattgaag ggtaccagag aactcagtcg ccttataaca
     3481 aatgacaata tagcgcccac ctgtatctag tggccaattt ttaatgccga caagttgagg
     3541 attttggata gtcaaccctg tttcttcgta gatttcacga atgacagact ccgcaaaagc
     3601 ctcatcattt tctacatgac ctccaggaaa ggcataacca gaccagcgat tgttttcagg
     3661 ggcgcgatac tgcatcacca cgcgctgagt ttcgaggtct tcaatcagac agatatttgt
     3721 taaaattgtt aattgggaac gggacataaa tttactcgct ttctaattta ttaaattttc
     3781 agacttacta ccagagatat tatcttttac ttttttaaaa ttatttagaa tagaacacca
     3841 ttcgtttttc ctttataggc tttccaagtt caagtatttt ttcttgtcca tcaaaagtaa
     3901 aacccatttt ttgatagaaa gcaatggcgc gcttgttatc tttcaatacc cataagaaaa
     3961 tttcagaaaa atgattaaga gcagtcaaag ctgctttcat taacttttgt gcgatacctt
     4021 ttccataata gtcttttaaa acatataaag caataatttc accagcttga atagtctcat
     4081 cacgaaaatt gccataactg ataaaaccaa ccaccttcac gccatccatc gcaatcaatg
     4141 tattttctgg atacttttga ctaaagaatc gacatctttc taatgtcatt gtatcctgaa
     4201 attccgcagg caaaaggtca tcataagact ctctccacgt ttgccagtga acgagggatt
     4261 tgccttctat ctcttcagga gtttccattg atttgataat aaccgtcatt tattttctcc
     4321 cagtcttctc tcaaaatacc atatttaata ctatcaaaat atttaccttg ataataacga
     4381 acttttggaa tatgagcttc ttttttcatt cttaattttt cagcaagttt catcatacca
     4441 agatttcctg accaagttgt caaaaccaga tgctccaact ccaagtaatc ctgaaacgtc
     4501 ctatctatcc actgcaacat agcaactttc ccaataccag tgttccagaa ttttttatca
     4561 taaataccaa ttcccaattc catccatctt gtttctttac atacccaata acgcgaaaca
     4621 gtcccaacta gtttatcatc aacaaaaata ccaaggcgat ttgagttgct taaaatggat
     4681 tctgattttt gtagttcgaa ttctttaaaa tttgaaaaat actgataatc atcataatag
     4741 ggagcatcat actgtttcca aactggatta agttgtgaat aagccatttc ccataaagat
     4801 atcaaatctt ctctctttaa ctttcttaaa taaatcatac tattccccta atccaaggcc
     4861 ttgacttcca acatcatatc acggatctga gccgccagtt caaagtcaag cacttcgacg
     4921 gcttcttgca tttgtttttc tagtttcttg acgagttctt tgcgctcttg tttgttgagg
     4981 ctattgatgt cgacttcctt gtcctcttcc ttagcaactg ccttggtcac ggcaatcagg
     5041 tcacggattt ctttcttgat tgtctgtggt acgataccat gctcttcatt ataggccatc
     5101 tgaattttcc gacggcgggc agtttcatcg atagcacgtt gcatagactg ggtaaccgtg
     5161 tctgcataca tgatgacatg gccttcactg ttacgagcag cacgtccaat agtctggatg
     5221 agtccacgtt cgttgcgaag gaaaccttcc ttgtcagcat cgagaatagc tacgaggctc
     5281 acttcaggaa cgtcaattcc ttcacggagc aggttaattc cgaccaagac atcaaagaca
     5341 cccaagcgca ggtcacggat aatctccgtc cgttccaagg tcttgatatc cgagtgcatg
     5401 tacttgacct tgatacccat ttccttgaag tagtcggtca aatcctctgc cattttcttg
     5461 gtcaaagttg tgataaaggt acgctcattt ttttcaacgc gggcattgat ttcacccaag
     5521 aggtcatcaa tctgtcccat agtcggacgg acttccacct ctggatccaa gagtcccgtt
     5581 ggacgaatga tttgctcaat cactgtctcg gtctgttcat tttcatagtc accaggtgtc
     5641 gctgaaacgt aaacaatctg atgaacgtga ctctcaaact cctcccgacg gagaggacga
     5701 ttgtccaaag cagacggcaa acggaaacca taattaacca gcatttcttt acgcgaacgg
     5761 tctccattgt acatgccctt gatttgccct atggtcatat gactctcgtc aatcataatc
     5821 aagaaatcat ctgggaagaa gtcgagaagc gtataaggag gctctccttc gctccgtcca
     5881 tccatgtggc gagaataatt ttcaacccca ttggtatagc ccatctcacg caacatttcg
     5941 atatcatact ctgtccgctg tttcaaacgc tgggcttcaa gcagtttacc ttccttttca
     6001 aagacagcta attgttcttc caactcggcc tgaatctttg caatggcaac ttccatgtgg
     6061 tcgtcattgg tcacaaagtg tgtcgctggg aaaatcgcta aatgatccac ttctcccaac
     6121 acctgacctg tcagagcctc aacttcacga atacggtcaa tttcgtctcc aaaaaattct
     6181 actcgaaagg catgttcatc tcgggaagct gggaaaatct ctaccacatc cccacgaacg
     6241 cgaaatcttc cgcgttggaa atcaatatca ttgcgttcaa actgaatatc gaccaagtca
     6301 ttcaagagtt tatcacgaga aatctctaga ccaggacgga gactaacgac actatcagcg
     6361 tattccttgg gcgaacccaa accatagata caagagactg aggccacgac aataacatca
     6421 ttacgctcca aaagggctga ggtagctgag tggcgaagct tgtcaatctc gtcattgaca
     6481 gaactatcct tctcaatata ggtatcgcta gaagggacat aggcctctgg ctggtaataa
     6541 tcatagtagg atacgaaata ctcaactgca ttttcaggga aaaattcctt aaactcccca
     6601 tagagctgac cagccagagt tttattgtgg gcaataacca gagttggttt attgacttta
     6661 gaaatgacct gactcatagt atacgtcttc cctgttccag tcgcccccat cagaatctga
     6721 gctttttctc ccccctcaat gttatccacc aactgctcga tagcttgggg ttgatctcct
     6781 gatggttgat attttgatac tagtttaaat tgattatctg taatgtgatt gatcataaga
     6841 atcctctctc catgtgctat tcctatttta gcatacttat agtattttca cgaagaaaag
     6901 gatggaccga agtcacatcc tttcattttc tttctttaat ttattagcaa gatagacaat
     6961 cagcagtagt aataccagac ttgtcataat ggaaccttct atgccaaaag aaccacctga
     7021 tagccaatct tgattgcctt gtggtaaaaa ggtcatcaga gacgttcctg acggttgacc
     7081 actaactaaa atcccaaaga gattaccctg agcaaaattc caagcaccat gaatacctgc
     7141 aacaccccaa actgtatcag ttttgagaag gtaaagagcc atggcaactc cgaataaaaa
     7201 gagatttact agagatagag gggtgaggcc agaattgccc atatgaagca gggtaaagaa
     7261 caggctagat ataagaatag ctagttttag attggttctt gaggccaatt gaggaagtag
     7321 ccaagcacgg gccaccactt cttctgctgt cccctgtaaa atccaaaatg ggatagtaaa
     7381 gacgacaaag gcaagagaat aaggattcaa gtgaatggat tccaaacgat attgacctaa
     7441 gaccaccaaa cctaacaagg tcagaagaaa aagtgccaga cctagactaa atcctttcag
     7501 aagattgctg aggaaattct ctctataaaa tcccaaggtt cgaataggtc ttttctcaac
     7561 ttttcttgtc catctgaaca cagtgttaag aataaaacca aaggccagta atcctaaaat
     7621 taaacgaaag tcacttgttt tatccgtcaa gctctgatgc aaggtctgca agtagcttgc
     7681 aagattttga ccagcctctc caaaatttct agcaagtccg atgagagcta acaagccaat
     7741 accatacaaa gtaaaagcca caaactctcc tatgaagaca aaaacaaagg ctaacaaggg
     7801 gcttgtgtaa atgtttaaac tcatttttga agcttggaag tctttaaata ttcttttgtt
     7861 cttcatatcc ccgtcctctt ttc
//
LOCUS       DAVKAO010000075         7875 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKAO010000075 DAVKAO010000000
VERSION     DAVKAO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.075"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            24..344
                     /locus_tag="VWX16_001753"
     CDS             24..344
                     /locus_tag="VWX16_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9159060.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            509..1835
                     /locus_tag="VWX16_001754"
                     /pseudo
     CDS             509..1835
                     /locus_tag="VWX16_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2124..2594)
                     /locus_tag="VWX16_001755"
     CDS             complement(2124..2594)
                     /locus_tag="VWX16_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU9159061.1"
                     /translation="MRMPLKRPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(2560..3366)
                     /locus_tag="VWX16_001756"
     CDS             complement(2560..3366)
                     /locus_tag="VWX16_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9159062.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQNGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKKSGELDKLIEDAFKASIEK"
     gene            complement(3573..4214)
                     /locus_tag="VWX16_001757"
     CDS             complement(3573..4214)
                     /locus_tag="VWX16_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9159063.1"
                     /translation="MIELENITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(4211..6319)
                     /locus_tag="VWX16_001758"
     CDS             complement(4211..6319)
                     /locus_tag="VWX16_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9159064.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSLPLLVLIFFAHALLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(6371..6658)
                     /locus_tag="VWX16_001759"
     CDS             complement(6371..6658)
                     /locus_tag="VWX16_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9159065.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            complement(7042..7629)
                     /locus_tag="VWX16_001760"
     CDS             complement(7042..7629)
                     /locus_tag="VWX16_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159066.1"
                     /translation="MNYNLKYLLSGIFVLVFIGFLVWMRYFNQRKEEEHSDVSFEARL
                     DSEVKTLYKQLGLGEEPHYFLAYRYLHPWFDLAILPPTVEIFLTKIALVMVTPDELLI
                     RNLGNGLTFTSEHHRDLRQGLIRIPKSEMKEFEIRNWKKFFVFGDFLTIKTSQHSYYL
                     QVRDDGLQKGSLSTKHFSDLKSQDFLGLLTDKRTF"
BASE COUNT         2477 a         1711 c         1264 g         2423 t
ORIGIN      
        1 tttgtcaata atccgagaaa atcttgcaac gcttagaagt ctataaaaac tatcaacatt
       61 tatatgactt gcgaatagca atcctgctca acctttccac actctatcta tacaatcaag
      121 ataaaaacat gtgtaagcaa atctgctaca ctttactgga ggacgccaag aataagaaaa
      181 gctacgatag gctggctatc tgctatgtcc gcattgggat ttgtagggat gatgcgaaac
      241 ttatccaaaa agggttctca ctcctagagc tgaccgagga aacttctatg ctgtctcatc
      301 tcaaaaaaga agtagagatc tattaccaag cgaaggaaag ataaaaaagc cgagggattt
      361 cctcgactct ttacatatta tttgtggctc tataatattt gtagtgggta acccccctat
      421 ggatattatg gagcctattt tgtgtagaaa aaaagtccca tatgacctat aatgaaaagc
      481 gacaaaacaa ctcattagaa agattcatat gggacaatta cattttatca caaaactact
      541 cgatatcaaa gacacaaata cccaaattat agatgtcgtc aatagggatt cacacaaaga
      601 aatcatcgcc aaactggact atgaagctcc atcttgtcct gagtgcagaa gtcaaatgaa
      661 gaaatattac tttcaaaaac cttctaaaat tccttatctt gaaacaactg gtatgcctac
      721 tagaattctc cttagaaagc gtcgtttcaa gtgctatcat tgttctaaaa tgatggttgc
      781 tgaaacttct atcgtcaaga agaatcatca aatccctcgt atcatcaacc aaaagattgc
      841 tcaaaagtta attgaaaaga tttctatgac cgatattgcc catcagcttt ccatctcaac
      901 ttcaactgtc attcgaaagc tcaatgactt ccactttgag tgtaatttta gaaatctgcc
      961 taagattatg tcttgggacg ttgaaacagt ccggggagtg actgtttcaa tcgggagatg
     1021 gagatgagct ttattgcgca agattttgaa aagctcgata tcatcactgt tcttgaaggt
     1081 agaacacaag ctgtcatccg agatcacttt cttaaatatg atagagccgt ccgatgtcgc
     1141 gtcaaaatca ttactatgga tatgtttagc ccttactatg acttggctaa acagcttcgc
     1201 tttcaaattt ctaggctcag gctgaaacag tctcctagac tgtttcactc ccgaatgcta
     1261 aaatcgttct tgatcgcttt cacattgtac aacatcttag ccgtgctatg agtcgtgtgc
     1321 gtgtccaaat catgaatcag tttcatcgaa aatcccatga acacaaggct atcaagcact
     1381 actggaagct catacaacag gatagccgta aactgagtga taagcgattt tatcgtccta
     1441 cttttcgtat gcacttaacc aataaagaga ttttaaacaa gcttttgagt tattcggaag
     1501 acttgaaaca ccactatcag ctctatcagc tcttgctttt tcacttccag aataaggaac
     1561 cagagaaatt tttcgaactt atcgaggaca atcttaagca ggttcatcct atttttcaga
     1621 ctgtctttaa aaccttcctc aaagataaag aaaagattat caacgccctt caactacact
     1681 attctaatgc caaactggaa gcgaccaata atctcatcaa acttatcaag cgcaatgcct
     1741 ttggttttcg aaactttgaa aacttcaaaa aacggatttt tatcgctttg aacatcaaaa
     1801 aagaaaggac gaaatttgtc ctttctcgaa cttagctgac ttcaacccac tacagttgac
     1861 aaagagcctt atttgttcgt tagttcttaa taccagccat tgttaaacca gaagttttta
     1921 gctctgccaa gccacatagt gtgtttacta ctatgtgtcc atgtcaccat gaatcgccga
     1981 acgatttaac gagaaagtat gacttttacg tttatcccaa ctcaattatg acattttttt
     2041 caaaagtcaa tatatctcac tttttcaacg acaagaaaga ggctaccaat cgaccaacct
     2101 cttattctga acccatcact ccatcacttt ttagcttcat tcgctttctt agcgactgca
     2161 atctggtatt cgacttggtc attcccctta ccggtacagc catgagcaat tgtagtcgct
     2221 cctgtctgat gcgctatttc aactaatttt ttagaaataa gagggcggct caaggcagat
     2281 accaaggggt acttttgttc ataataggca tgtgactgaa gagccactag cacataatct
     2341 gtagcaaatt cgtccttgac atcaatgaca taagattcga ctgccccaac cttgagagcc
     2401 ttatcatgga tgaagtctaa gtctttccct tcacccacat ccatacaaac tgaaacaaca
     2461 tcatagtctt tctttaacca ggtaatagca actgatgtgt ccaaaccgcc agaataggcc
     2521 aaaattactt tttctttact catgttcttt tccttctttc tatttttcaa tggacgcttt
     2581 aaaggcatcc tcaatgagtt tgtctaattc tccagacttc ttcaactttt gaatggtttt
     2641 atcgactgcc tctttcaatt ccttgctatc ttttttcatg gctaccgcgt aggaatcatc
     2701 ttgctctttt tcaaaattga ggtctgcgat tgctaaatca ggattatttt ccacaaatcc
     2761 cttggcaaca ggttcttcaa agataacggc atccacttgt cctgatttta aatctgtgat
     2821 taaattccca tttttaggca gagatacgag ggaagaattt tgtagcaagt ctttcgccat
     2881 cgtctcttga atcgaaccat tctgtgctcc gacctttttc tgcgctaagt cgttgacaga
     2941 ctgataagtg gccaagtcag attttttgac aatgagttta ttttttgcag tatagtaggg
     3001 agttgaaaaa tcgaacacct tgctccgttc atctgtctta gaaacaccag atatggcaag
     3061 atcggctttt cctgattgaa cactagccag tacattgtca aaactcatgg gagatagttc
     3121 caattcgaca cctagttctg ttgcgatagc tttggctaat tcgatatctg aacccacaat
     3181 ctgatttttc ccatctacca ctttttgata ttcaaatgga gcaaaatctg gattgagagc
     3241 tacaaccaat tttcctttgg acttgatggc ttcaattcca gccgattgat tgttgctaca
     3301 agcaaatagt agggggagca tcactaaacc aaacatcgtc atcaacacct tcttcatctt
     3361 attcataaca aagcctcctt tatgtttatt ctttttgctt gtataaataa tactcctata
     3421 ctcatccttt gtcaagtcta aattgaaaaa attttcaatt tgaaacaaaa aaacctagaa
     3481 atactacata aaaatgtcat ttctaggcgt atttatgcta tttctctctg gaaaatatga
     3541 gtattcagtc agtcaaaagg agctgaacga actcatttcc cctcgcccaa ttcaatgatc
     3601 cgatgacatt gttgggctac ataagcatcg tgggtcacaa taatgactgt tttcccctct
     3661 cgattcatct ctaagagaaa cttcaagacc aaatctctat tttcagggtc cagagaacct
     3721 gttggttcat cggctaaaat cagctggctg ggttttaaga tggctctagc aactgcaatt
     3781 cgttgttgct ccccaccaga taactcggaa accttttggt gcaaagtagc tgacaaacct
     3841 accctctcta aaatctcttc cacctttttg agcttgtctt tcttagacaa tttcacatat
     3901 ttcagcgcca gcataagatt gtactcgacc gtttcatcat caatcagggc aaaattttga
     3961 aacagataag agatatgttc acggattatt gtttgcgact tagcagaatt aactgctaga
     4021 tttgtctgac caaaaatctc ataccgtccg ctataatcac catctatcaa acctaataaa
     4081 tttaacaagg tcgacttacc actaccactc ttgccaacaa tggctaccaa atccccctga
     4141 tcaatcctga gagataagtt atccaaaatc acttttcctc caatggtttt ggtaatattt
     4201 tccaactcaa tcataagatg ccccctttca ataactctac tagacttctt ttctccatcc
     4261 tagaagccaa gcctagcaca aatagtatat ccaaacatgt aaaacctgca aaaagtagga
     4321 gtggcaagag cgcatgggca aagaaaatca agactagaag aggcagacta tagcccagca
     4381 agagcagaac gaggagaggg cggtagcgat cgaccagttt ccaccccata aacttcttgg
     4441 taataatatc cctgcgcttc aataagaaag ttattactag taagaagtag gaaatcatca
     4501 tgctaaggag accaaacaaa gcaaagagta ggttaaaatt ccgaacagca tctcgataag
     4561 aatccacttt ctcttgttga atggcttgaa tagatgaaaa ttttaaataa tttccatctg
     4621 acaatttctc aactaactct gtaatctctt tttgatgttg aaccgtattt tcaattttaa
     4681 tcggattatt taagccagtt gttgacaggg aggctttctc atcccacatc atatcagaat
     4741 cattgaccaa gctaataatt ggattggaga gattttcctt tcgcttatca ctatatggga
     4801 aaaatgacca atctccttca taataggcaa tctcgacatc catctcctct atcgttcgtt
     4861 tttgctgctc ttcatacttc atcgaatgaa aggcaattaa cttccccaag agctgatttt
     4921 tatcttcttc acctttcgta cttgctggca tcaaaataac ttttttaata ccggtatctg
     4981 gtagcttgaa tcccttgctc tttagaaaat tgcgattggc atagtaaaca tccaccgtat
     5041 ctgttaactg atattgctga atctgttctg attggacaaa attttttaca ggaagactgc
     5101 tactctgcac atagcccgcc tgcgtttttt ctaccaaatc ctgataaaat cgatagaaat
     5161 aatccaatgc cttccctgag cctgccaact gctcttgcca caagttatca ttgagttgga
     5221 aagtttctaa ggtcagataa tttccttggc tgatccactg tttctggtaa tccaactctt
     5281 tgttttcttg ctccaaactt tttcctaccc caactagcaa gactgtcagt aaaatggtag
     5341 ttccaatttt catcaaataa ttaaagagta gaccaaattt gaaagatgaa aaacctttca
     5401 gcagagagct gattgtcatt ttttggatta agaggtaagt cagccaactg ataaagagat
     5461 aaagctgcaa cagcaaaaaa tgagacaacc acagcatagg aaacaaatct tttggcttat
     5521 aatcaagcaa gaaaaacacg cctagattga tcacaagagc cccacctagg aggaggtaaa
     5581 ggttgccttt tacaacatca gctaaaacag ccctatcttg aaaaccaagt aatttttgta
     5641 ccccaactcg tttcatctcc atcatcggtt gatacactgt cactaacaca agaagcaaaa
     5701 tagccaagac aaaaacaatg gcagataaaa gcaaatctcg atttatgact tccactgcac
     5761 ttttgtaggt cggctctagc aaggtagcct ggtctatctt gaaaaaatcg ctccatttct
     5821 gtacaatcct atccttgtcc atctcttgtg tagaagttat cgtatagcga ccatttaaac
     5881 tacgagatgt atccttgata taggtttgaa aagtcataag ctgaataggt ttggctttta
     5941 gaaaggtcgg aatcgtacca agtttattgg aaatttcttt attactatag actccttcac
     6001 catctgtggt aaaatcaaga gaagaaatcc caaactcttg gtaggggaag gtatctttat
     6061 caaaaacacc agacttgacc acctcatcac cactgtctgt tttgatgatg gagactttat
     6121 actcctttga tacatcctca aaaaatcgaa gaacagacgc tgcaggttcg ttaatatctt
     6181 tcaaatacaa atccaaagaa tctacagtct ttcccatttc ttgaatccga accacttctc
     6241 gtgtatagtt tacattaaaa acaaaaaaag tggtacacag aagcaggagt aacatacaga
     6301 aattactgat ttttttcata aaagtgcctc cactacagtt ttccctctcc aaataggaga
     6361 gggaagaatt ttactcaaat ccatagaaat attccattcc tgttggcgga atcttggtga
     6421 gggaagctct agcccatgcc tcaggttttg cataatcctt agaggttcta cccccatgtc
     6481 taacagttgc tgtatgatat cgagtagaat gtaagtaatc agaatatcca aaagttcctg
     6541 tccaacctac tccatagttc cattgaccac cctcaaccca tactgctaaa gcatttgtag
     6601 tagcaagtat accacatacc attaaagatg caacaccaat tttaactaat tttctcatga
     6661 tttatttctc ctattatcga gagctgttcc gtaaaaacag ctatagtaat ttatcaacaa
     6721 gtatcgtagt ccattggact caccttcctt ctatctcatt tcatttttta acaacgaact
     6781 tactcttaac cgggagaatg gtgcgcttct gtatcaggtc cttgaacaac cagagtagta
     6841 ttaagcacac tctaactatt actcaagaat cagtccaata aacaatatgg atagcgcttt
     6901 caaattttta aactcccatc tcatcctttt atctatatta taagtgaaaa tatgataact
     6961 gtcaagtaat tgaagtgaat tttataaaaa attacaagcc aaatttgtaa agtttacaat
     7021 aagccctagg gcaattgtca atcagaatgt ccttttatct gtcaacaatc ctagaaaatc
     7081 ttggctcttc aagtctgaga aatgtttggt agaaaggctt cctttttgaa gtccatcatc
     7141 tctaacctgt aaataatagc tatgttggct tgttttaatt gtcaaaaaat cgccaaatac
     7201 aaaaaatttt ttccagttac gaatctcaaa ttctttcatc tctgattttg gaatgcggat
     7261 aagtccttgc cgcaaatcac gatgatgctc acttgtgaaa gtcagcccat tcccaagatt
     7321 tcttatcagt agttcatctg gagttaccat caccagcgct attttagtta agaaaatttc
     7381 aactgttggt ggtaaaatcg ccaaatcaaa ccaaggatgt aggtagcgat acgctaagaa
     7441 ataatgaggc tcctcaccca atcctagttg tttatataag gtcttgacct cactatctaa
     7501 cctcgcttca aaagacacat ccgaatgctc ttcttccttc ctctgattaa aataacgcat
     7561 ccagactagg aaacctataa agactaggac aaatattcct gataaaagat attttaaatt
     7621 gtagttcatc acttaacctc ctgattcacg cttctgccta aaacagaggc ctatttattt
     7681 tgaaaatagt tccatcataa ctttaaataa gacgggtgtc attaaaatta ggataattcc
     7741 tatcacaaat atcatgacta ctcgaaaaag ttccttattt ttaaacattt ttccacccct
     7801 tttctagcta catcatagta ctcattaacc ccatattaaa attacaccaa acttatttta
     7861 tctttctttt ttact
//
LOCUS       DAVKAO010000076         7488 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKAO010000076 DAVKAO010000000
VERSION     DAVKAO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.076"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..180
                     /locus_tag="VWX16_001761"
     CDS             <1..180
                     /locus_tag="VWX16_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159067.1"
                     /translation="FNHSIKNIEYIKARNIKTSSDSDIELESDVDGDKSDNLPVDIKI
                     LGQHIEVYSVPKKQS"
     gene            380..3655
                     /gene="rexB"
                     /locus_tag="VWX16_001762"
     CDS             380..3655
                     /gene="rexB"
                     /locus_tag="VWX16_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9159068.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAEKLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLKKLDLADSKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3652..7302
                     /gene="addA"
                     /locus_tag="VWX16_001763"
     CDS             3652..7302
                     /gene="addA"
                     /locus_tag="VWX16_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9159069.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVELKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNSQKWL
                     SDSFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGRLILLKENFRSSSEVLSTTNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALKMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEAQVVKV"
     gene            complement(7264..>7488)
                     /locus_tag="VWX16_001764"
     CDS             complement(7264..>7488)
                     /locus_tag="VWX16_001764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159070.1"
                     /translation="CFEVVDRTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNH
                     IYSKAKLTWFEEIFKEYKLYFYNLSLVFTE"
BASE COUNT         2270 a         1601 c         1590 g         2027 t
ORIGIN      
        1 ttcaatcatt ctatcaagaa tattgaatat ataaaggcac gtaatattaa aacttcttct
       61 gattctgata tcgaattaga gtccgatgtt gatggcgata aatcagacaa tctacccgtc
      121 gacattaaga tactgggaca acacattgaa gtgtattccg tacctaagaa acaatcataa
      181 aactcaaagc cttgttaccg aggctttttt attatgtgat tttcagtagc cagacttatt
      241 ctatctaata caagtaaagc taagtattta agatttagca gatgatgaag aaaagaaagg
      301 atggcctaaa tattttaaca gatttacgag gctaatcatg gtataatata gggtgatgaa
      361 aaattccaac gaggctgaga tgaaattact ttatactgat attcgaactt ctttgacaga
      421 aattctaaca agagaggcag aagagctagt tgcagctggc aagcgggtct tctacattgc
      481 ccccaactct ctttcttttg aaaaggaacg cgccgtgctg gaatgcttgt cccagcaggc
      541 ttctttttcg attaccgtca cgcgctttgc tcaaatggct cgttatctgg tcttgaatga
      601 tttaccagtt aaaactactc ttgatgatat cggtcttggg ttggcctttt acaaatgcct
      661 tgccgaactc gatcccaagg acttgcgtgt ttatggcgct attaagcagg atcctcaatt
      721 gatccagcag ttaattgagc tttaccatga gatgaccaaa tctcagatga gttttttgga
      781 cttggagaat ttaacagatg aggataagag gacggattta ctcttgattt ttgagaaagt
      841 aacagcctat cttaatcaag gtcagttagc ccagggaagt cagttgtccc atttgattga
      901 ggctattgag aatgacaagg taagtagtga ttttaatcaa atcaccttgg tcattgacgg
      961 ctttactcgt ttttctgctg aggaagagcg ggttgtggac ttacttcacg gcaaaggtgt
     1021 tgagattgtt atcggggctt atgctagtaa gaaagcctat accagtcctt ttagcgaggg
     1081 caatctctac caagccagcg taaaatttct ccatcatctg gcttctaaat accaaacgcc
     1141 tgctcaggac tgttctcaaa ctcatgagaa gatggatagt tttgacaagg cctctcgttt
     1201 gttggagtct tcttatgact tttcagaact cgctttggat gtcgatgaga aagaccgtga
     1261 aaatttacaa atctggtctt gtttgacgca aaaggaggag ttggagctag tagcccgtag
     1321 cattcgtcag aaattacatg agaactcaga cctgagctac aagcattttc gtattctctt
     1381 gggggatgta gcttcttacc agttatctct caaaaccatt tttgaccagt atcagattcc
     1441 tttttatctt ggtagaagcg aagccatggc tcatcatccc ttgactcagt ttgtcgagtc
     1501 tattttagct ttaaaacgtt accgttttcg tcaggaggat ttgattaatc ttcttagaac
     1561 tgatttgtat actgacctca gtcagtctga tattgatgct tttgagcaat atatccgcta
     1621 tcttggtatc aatggcttgc cagcctttca gcaaaccttc accaaatccc accatggaaa
     1681 atttaatctt gagcgtttga atgtcctccg cctgagaatt ttagcacctc ttgaaaccct
     1741 ctttgccagc cgaaaacaaa aggctgaaaa actcctacaa aaatggagtg tctttctaaa
     1801 agaaggagct gtgaccaagc agttacaaga tttgacaacc actttggaag ctgtagaaca
     1861 ggaaagacaa gccgaagttt ggaaggcttt ctgccatgtt ttagaacaat ttgcgactgt
     1921 ttttgctggt tcacaggtta gtctggaaga cttcctagcc ttgctccatt ctggaatgag
     1981 tttgtcccaa taccgtacca ttccagcaac agtggacact gttctggtgc agagttacga
     2041 tttgattgca ccattgactg ctgactttgt ctatgctatt ggactaactc aggacaattt
     2101 accaaaaatt tctcaaaaca ccagtcttct gacagatgaa gaaaggcaaa acctaaacca
     2161 agcgaccgaa gaaggcgttc aattactgat tgccagcagt gaaaatctca agaaaaatcg
     2221 ctacactatg ctttccttgg tcaattctgc tcgtaagcag ttgttcttgt cggctccaag
     2281 cctttttaac gaaagtgaaa gtaaggaatc tgcctatctt caagagttga tccattttgg
     2341 atttaggcgg agagagaaga ggatgaatca caaaggactg tctaaggagg atatggggtc
     2401 ctatcacagt cttttgtcta gtctggttgc ctatcaccag cagggtgaga tgagcgatac
     2461 tgagcaagat ttgacttttg tcaaggttct gtcgcgtgtc ataggtaaaa aactagatca
     2521 gcaaggtctg gaaaatccag ccatcccaat cagtccaagc agcaagacct tagccaagga
     2581 caccttgcaa gctctctatc ctgccaaaca ggagttttac ctgtctacgt cgggtttgac
     2641 agagttttat cgcaatgaat acagttattt cctacgctac gttttaggct tgcaggagga
     2701 attacgtttg catcctgatg cccgtagtca cgggaatttc ttgcatcgta tctttgaacg
     2761 cgccttacag ttgcctaatg aagattcctt tgaccaacgt ctagaacaag ctattcaaga
     2821 aaccagtcaa gaacgcgaat ttgaagctat ttatcaagaa agtttggaag cccagtttac
     2881 caaggaagtt ttgcttgatg ttgcacggac gactggacat attctccgac acaatccagc
     2941 catcgaaacc atcaaagaag aagcaaattt tggtggaaaa gaccaagcct ttattcaatt
     3001 agacaatgga cgcagtgtct ttgtacgagg caaggtggac cggattgacc gtttgaaagc
     3061 taatggagcg ataggagtag tagactacaa atccagtctg actcagttcc agtttcctca
     3121 tttctttaat gggctcaatt ctcagttacc aacctatctt gctgccctaa aaagagaagg
     3181 ggagcagaac tttttcggcg ccatgtactt ggaaatggct gaacctgtcc aatctctgat
     3241 ggcggtaaaa agtcttgcag gagcagtggt agaagccagc aaatctatga aataccaagg
     3301 gctcttcttg gaaaaagaaa gcagttattt aggcgaattt tataacaaaa acaaggctaa
     3361 tcaactgaca gatgaggaat ttcagctcct actggactac aatgcctatc tttacaagaa
     3421 agctgctgag aagattttag caggccggtt cgccatcaat ccttatactg aaaatggcag
     3481 aagcattgcc ccatacgtcc agcaacatca ggctattaca ggctttgaag ccaattacca
     3541 tctgggccaa gcccgtttcc taaaaaagtt ggacctagct gatagcaagc gtctggtcgg
     3601 agaaaaactc aagcaagctt ggcttgaaaa aataagagag gagttgaatc gatgaagctt
     3661 attccctttt taagtgagga ggagattcaa aaactgcaag aagcagaagc aaattcgagc
     3721 aaggaacaga agaaaactgc cgagcaaatc gaagctatct acacttctgg ccagaatatc
     3781 ctggtctcag catcggctgg ttctggaaag acctttgtca tggcagagcg cattctggac
     3841 caattggcgc gtggtgtcga aatttctcaa ctctttatct caacctttac cgtcaaggct
     3901 gcaactgaac ttaaagaacg tttagagaaa aaaatcagca agaaaatcca agaaacagat
     3961 gatgtcgagc tcaaacaaca cttgggtcgc cagttggcag acctacccaa cgctgccatc
     4021 ggaaccatgg actctttcac acaaaaattc cttggcaaac atggttatct gcttgatatt
     4081 gcacctaatt tccgtatttt acaaaaccaa agcgagcaac ttcttctcga aaacgaagtc
     4141 tttcatgagg tctttgaagc gcattaccaa ggtaaacaga aagagacctt tagtcatttg
     4201 ctgaaaaact ttgctgggcg tggcaaggac gaacggggtc tgcgccagca ggtctataaa
     4261 atctatgact tcctccaatc caccagcaat tctcaaaagt ggctgagtga ctctttcctc
     4321 aaaggctttg agaaagctga ttttaccagt gaaaaagaaa aactgactga gcaaatcaaa
     4381 caagcccttt gggatttgga aagctttttc cgttaccatc tggataacga tgccaaggag
     4441 tttgcaaagg ctgcctattt agaaaatgtt cagttaattc tggatgaaat ttgctcccta
     4501 aatcaggagt ccgatagtca ggcttatcag gcagtgcttg cgcgtgttgt cgccatctct
     4561 aaggagaaaa acggtcgagc tctgactaat gccagccgta aggctgattt gaagcccctg
     4621 gctgatgcct acaacgaaga gagaaagacc cagtttacta aactaggaca attatcagac
     4681 cagatagcga ttctcgacta tcaagaacgt tatcatggag acacttggaa actagctaaa
     4741 accttccaat ctttcatgag cgattttgta gaggcttatc gtcagagaaa acgacaggaa
     4801 aatgcctttg aattcgctga tatcagccat tacaccattg agattttaga aaatttccca
     4861 caagttcgtg agtcttatca ggagcgcttc catgaagtca tggtcgatga gtatcaggat
     4921 accaaccata ttcaagaacg gatgctggaa ttgttgtcta atggccacaa tcgctttatg
     4981 gtgggagata tcaagcaatc catctatcgt ttccgtcagg cagacccgca gattttcaat
     5041 gagaaattcc aacgctatgc gcaaaatcct caagaaggca ggctcattct cctcaaggaa
     5101 aatttccgta gtagttcaga agtgctgtca acaaccaatg atgtctttga acgtctcatg
     5161 gaccaagagg tcggcgaaat caactatgat aacatgcacc agcttgtttt tgccaatacc
     5221 aaactgactc ccaatccaga caacaaggca gaatttctcc tctacgacaa ggacgataca
     5281 ggtgaggaag aagagagtca aacagaaacg aaactaacag gcgaaatgcg cttagttatc
     5341 aaggagattc tgaaacttca tcaagaaaaa ggtgttgcct ttaaggaaat tgcccttctg
     5401 acctccagcc gcagtcgtaa tgaccagatt ctcctcgccc tgtctgagta cggaattcct
     5461 gtcaaaactg acggagagca aaacaattat ctccaatccc tagaagtgca agtcatgcta
     5521 gacactcttc gtgtcattca caatcccctg caagactacg ccttggttgc ccttatgaag
     5581 tctccaatgt ttggttttga tgaggatgag ctagcacgtt tgtcccttca gaaagcagag
     5641 gataaagtcc acgaaaatct ctatgagaaa ctggtcaatg cacaaaaaat ggcaagtagt
     5701 caaaaaggtt tgattcacac agctctagct gaaaaactaa agcaattcat ggatatccta
     5761 gcttcttggc gcttgtatgc caaaacccac tctctctatg acttgatttg gaagatttac
     5821 aacgaccgtt tttattatga ctatgttggg gctttgccga atggtcctgc taggcaggcc
     5881 aatctctatg ccctagcact gcgtgctgat caatttgaaa agagcaattt caaaggtttg
     5941 tcgcgtttta ttcgtatgat tgaccaagtc ttagaagccc agcacgattt ggcaagcgtg
     6001 gccgtcgcac cgccaaaaga tgcagtagag ctcatgacca tccacaagag taaggggctg
     6061 gagtttcctt acgtctttat cctcaatatg gatcaagatt tcaacaagca agactctatg
     6121 tcagaagtca ttctcagtcg tcagaatggt cttggtgtca aatatattgc caagatggag
     6181 acaggggcag tagaagacca ctatcctaaa accatcaaac tctccattcc tagcctgacc
     6241 tataggcaga atgaagagga attacagcta gcaagctatt ctgagcagat gcgtttgctg
     6301 tatgttgcta tgacgcgggc tgagaaaaag ctctatcttg tcggcaaggg ttctcgtgaa
     6361 aagctggaag ccaagcaata cccagcagcc aaaaatggga aactaaatag caatactaga
     6421 ctgcaagcac ggaatttcca agattggctt tgggctatca gtaaagtgtt tactaaggac
     6481 aagctcaact ttagttatcg ttttattggc gaagatcagt tgaccagaga agctatcgga
     6541 gagttggaaa ccaagagtcc tctccaagat agctcccaag cagacaatcg tcagtcagat
     6601 accatcaaag aagctctgaa aatgctgaag gaggtggaag tttataatac tcttcaccgc
     6661 gcagctattg aacttcctag tgttcaaacc ccaagtcaaa tcaagaaatt ctacgaacca
     6721 gttatggata tggaaggtgt cgagattgct ggtcaaggtc agtcagtagg caagaaaatc
     6781 agcttcgatt tgccagattt ttcaacaaaa gaaaaggtaa ctggagctga gattggtagt
     6841 gctactcacg aactcatgca gagaattgac ctcagccagc aactaaccct tgctagccta
     6901 acagaaacac tcaaacaagt tcaaactagc caagctgtca gagacaagat caatcttgat
     6961 aaaattcttg ctttctttga cacagtactc ggtcaggaaa ttcttgctaa taccgaccat
     7021 ctctatcgcg agcaaccttt ctccatgctc aaacgagacc aaaagagtca ggaagacttt
     7081 gttgtccgtg gtatccttga tggctatctg ctttacgaaa acaaaattgt tctgttcgac
     7141 tacaagacag accgctatga tgaaccaagt caactcgtag accgctatcg tggtcagtta
     7201 gctctatacg aagaggcttt atcacgagcc tattcgattg aaaatattga aaaatacttg
     7261 attttactcg gtaaagacga ggctcaagtt gtaaaagtat aacttatact ctttgaaaat
     7321 ctcttcaaac cacgtcagct tcgccttgct gtatatatgg ttactgactt cgtcagttct
     7381 atccacaacc tcaaaacagt gttttgagct gacttcgtca gttctatcca caacctcaaa
     7441 acagtgtttt gagctgactt cgtcagttct atccacaacc tcaaaaca
//
LOCUS       DAVKAO010000077         7471 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKAO010000077 DAVKAO010000000
VERSION     DAVKAO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.077"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(50..208)
                     /locus_tag="VWX16_001765"
     CDS             complement(50..208)
                     /locus_tag="VWX16_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9159071.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEEILVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(279..704)
                     /locus_tag="VWX16_001766"
                     /pseudo
     CDS             complement(279..704)
                     /locus_tag="VWX16_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(801..1013)
                     /locus_tag="VWX16_001767"
     CDS             complement(801..1013)
                     /locus_tag="VWX16_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9159072.1"
                     /translation="MTQLLLHADETSYRVLESDSQLTYYWTFLSGKAENQAITLYHHD
                     QRRSGLVVQEFLGDYSGYVHCNMLRQ"
     gene            complement(1022..1327)
                     /locus_tag="VWX16_001768"
     CDS             complement(1022..1327)
                     /locus_tag="VWX16_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEU9159073.1"
                     /translation="MFPVETEEITYKRKKSKGKCQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
     gene            complement(1314..1508)
                     /locus_tag="VWX16_001769"
     CDS             complement(1314..1508)
                     /locus_tag="VWX16_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9159074.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEEDGDVPS"
     gene            complement(1705..2028)
                     /gene="tnpB"
                     /locus_tag="VWX16_001770"
     CDS             complement(1705..2028)
                     /gene="tnpB"
                     /locus_tag="VWX16_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9159075.1"
                     /translation="MTIHLSILGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLTWLSTEKDIKALTSEQVDWLMKGFS
                     ITPKI"
     gene            complement(2012..2194)
                     /locus_tag="VWX16_001771"
                     /pseudo
     CDS             complement(2012..2194)
                     /locus_tag="VWX16_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2498..4060)
                     /gene="guaA"
                     /locus_tag="VWX16_001772"
     CDS             complement(2498..4060)
                     /gene="guaA"
                     /locus_tag="VWX16_001772"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9159076.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4202..4900
                     /locus_tag="VWX16_001773"
     CDS             4202..4900
                     /locus_tag="VWX16_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9159077.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKYGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4943..5839)
                     /locus_tag="VWX16_001774"
     CDS             complement(4943..5839)
                     /locus_tag="VWX16_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9159078.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGILQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(5848..6783)
                     /locus_tag="VWX16_001775"
     CDS             complement(5848..6783)
                     /locus_tag="VWX16_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9159079.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6780..>7471)
                     /locus_tag="VWX16_001776"
     CDS             complement(6780..>7471)
                     /locus_tag="VWX16_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteinase"
                     /protein_id="HEU9159080.1"
                     /translation="LKANNRKLNETFYIETLGMKALLEESAFLSLGDQTGLEKLVLEE
                     APSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFEIFSPEDD
                     LILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDIESFLESSEIGAS
                     LDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESIEYFIPKSEKFFLGKD
                     RNNVELWFEEV"
BASE COUNT         2272 a         1612 c         1378 g         2209 t
ORIGIN      
        1 aaatctccag actagagaac tcacggatag ttcctaatct ggagatttct tatttgcact
       61 tttcttgtac aactttagtc catggtaaat aggcctctaa aacctctttg tttacgagaa
      121 tttcctcgtt tggaagacat tctagaagat aggatagata tttctcacta tttagttgat
      181 gacgtttagc tgtttccaat aagctcataa taatagctct tgctttagct ccttcaaaac
      241 tttgagaaaa taaccaccct cgctttttta aactgagctc aggctaaaag agtccactgg
      301 actcttttac tccgtcccat aaccaatgac ttaatggcgc gttcagctag attattggaa
      361 aggaccagat gtccgtcttt caaaatagtc ttaaaggttt cttcatactt gaggctgtat
      421 tcaattgccc ttcctagttt tgaacccgat aaaactgact gacgccggca ccaagcaaag
      481 aaatcttcca ttaggggttg gagctcttct tgacgtttct gtagtcgttc atcagctgac
      541 aatgtctccc agtctctttc caaggcaaat aactgatcac aataggctaa tcctttagct
      601 cctaaggatg atttatccgc ttgcttgggg gtcgcttcaa aaaacttcct tctcacatgt
      661 acccaacatc caacaagttc agcttcttcc agttgacgat aagcttctag cggtttgcag
      721 tttaccaagc ttagcgtcgc cttgctccta agccttggac tgctatcgca taggcagaac
      781 tagaggacta aagttctaag ttactgccgc aacatattac aatgcacata gccagaataa
      841 tctcctagga attcttgtac tactaaacca ctccgacgct gatcatggtg gtacagcgtg
      901 attgcttgat tctcagcttt cccagacaaa aaagtccaat agtaagtcaa ctgactatca
      961 ctctctagaa ccctataaga agtttcatcc gcatgaagta agagctgagt caatagtctc
     1021 gttataaagg ggctttagga ataggagctt tcacgatttt atcactcgga tttttatcac
     1081 tgcatgcttg gcatttgtaa gcgtgttgga tatgatctat gcgttttaat tgcgcaggaa
     1141 taaagactaa ttcttgtcgt tgaagggttg ctccaatctc ttttagatct ccctgacaat
     1201 cagagcaaat gctctcttct acttgatgat gaacttcttc tgaatcaaat tgggcaagaa
     1261 gagcttggca tttcccttta gatttcttac gtttgtaggt aatttcctct gtttcaactg
     1321 ggaacatctc catcttcctc agatggagac tcttcctcaa aaaggttgag ttgtccagag
     1381 gggcaaacac ttttctcaga ggattttcca tagagctttt gagtcagata agccacttgt
     1441 tcacgcagaa gggcaagttc attggtgaga ctatcaattg tagcactctg ttgttgaatg
     1501 attttcaatc ttcttccatg agcttccctc tctgttttct aactttatta tactaaaaag
     1561 aaagtcctca tttcaataga aatcacgact ttctgatgga tttatagtaa aatgaaataa
     1621 gaacaggaca aatcgatcag gacagtaaaa tcgatttcta acaatgtttt agaagcagag
     1681 gtgtactatt ctagtttcaa tctactatat ttttggagtg atagaaaagc ccttcataag
     1741 ccagtctact tgttcagatg tgagagcttt gatatccttt tctgtactta gccaagtcag
     1801 tctgccgttc tcaaagcgtt tatatagtag ccaaaatcct tgaccatccc agtaaaggac
     1861 tttaaagcgg tctttacgtc caccacaaaa gagaaagact tgaccggaga aaggatccaa
     1921 ttcaaagtgg gttttaacca gataagccag tgaatcgatt ccttgtctca tatccgtttt
     1981 cccacacacg agatagacct gccctaggat agatagatga attgtcatag agcaacacct
     2041 tatccaaaag ctgttctacc atttcgagat tgagagattg aaaaaaactt acttcaagct
     2101 tgccaatacg aattttaagc agaatatcat ttctcttttt agaatcaaaa cgacgagatt
     2161 gtggaatctc tacaggaaca ataggttgtg gcattaaaat atcctccaat agttttactt
     2221 tactaatagt atacaggagg agctccatga atgatagata ccttgttatg acgcgcttac
     2281 tatattagaa agttggctct ctaattaaat taattctcat tatatatatc aaaagaccgc
     2341 agcttgaaaa ctacggtcta attttttgtc caaatgtaaa ccacttggaa ctattattca
     2401 atttattctt tttagtaaat gttcccgttt taatcccagt tacagttttt aatcctctgt
     2461 aaccgttgat tttacaaagc ttttaatttt tttgcgatta ttcccactca actgttgcag
     2521 gtggtttact tgtaatatcg tagacgatac ggttaacatg atccacttca tttacgatac
     2581 gtactgagat tttttgaagt acttcccatg gaattttggc aaaatcagca gtcataccat
     2641 cgatagaagt gatagcacgg attgcaatcg tgtagtcata cgtacgaccg tcacccataa
     2701 cgccgactga acgaacgcct gtgttaacag tgaagtattg ccaaatatcg cggtcaagtc
     2761 cagctttagc gatttcttca cgaagaatag cgtctgattc acgaacggtt tcaagtttct
     2821 cttcagtgat ttcacccatg acacggatag caagtcctgg tcctgggaat ggttggcgcc
     2881 atacgatatg gtctggcata ccaagctctg taccaagagc acgaacttca tccttgtaaa
     2941 gagtattgag tggttcaatc aattcaaact gcatatcttc tggaagacca cccacgttgt
     3001 ggtgtgactt gatagtttga gctgtatccg taccagactc gataacatct gtatataaag
     3061 taccttgagc aaggaatttc acatctttga gcttgcttgc ttcgtcatcg aatacataga
     3121 caaactcgtt accgatgatt ttacgttttt gttcagggtc agaaacgcca gcaagtttgt
     3181 caaggaaacg tttagcagcg tctgctttga cgatattcaa accaaactta ccaccgagca
     3241 tgtccataac ttgatcagct tcgcctttac gaagaagacc gtggtctacg aagatacaga
     3301 tcaattgatc gccaatcgct ttttggagaa gaaccccaac gacagatgag tcaacaccac
     3361 ctgatagacc aagaaggaca cgtttatcac cgacggtttc acgaattttt ttgatctgca
     3421 tgtcaatgaa attatccatt gaccagtcac ctttagcctt acaaatgtta agggcaaagt
     3481 tacgaaggat atcatttccg tatacagaat gacgaacttc tgggtggaat tggataccgt
     3541 aaatgtgttt atctgggttt tcgatggctg cgtatgggca gtcagctgat gtacctgtac
     3601 gaacaaagtc agcaggaatc tcagtaaccg catcaccatg gctcatcaaa acagtctgtt
     3661 catcaggtgt tgattcaaaa agcgctgatg gtgtgtgagt tagggttgat tgaccgtatt
     3721 cacgatttcc agcatcacct gcaggaacaa cttttcctcc aagtttatgg gtcaataact
     3781 gcataccata acagattccc aaaattggaa ttcctagttc gaagatttct gggtcaatgt
     3841 caaatgaacc atcttcatat acagaatttg gaccacctga tagaataatt cctacaggat
     3901 tgacttcacg aacttcagca gctgaaattt tatggctttt tagttctgaa aaaacaccaa
     3961 tctcacggat acggcgtgaa atcagctggt tgtactggct accatagtcc aatacgatga
     4021 ttttttctac atcttgcaaa tcagttgaaa tgttgctcat ctttttcctt tctcaaaaga
     4081 aatatctttt ctcaatattc tatcacaaat aagggcgaat taccaactaa acaaggcgaa
     4141 gtttgacttt ttctagttta ttggggtaca atagagaaaa gatagaaatg aagtgaaaaa
     4201 tatgctacct gcttatatga aaatccatga tcagataaaa aaggatattg acgagcaccg
     4261 ttgggctatt ggtgagaggc ttcccagtga aagagattta gctgagcagt ttgcggtcag
     4321 tcgcatgacc ctccgccaag ccgtatctct attagtcgaa gaaggcgtct tagagcgccg
     4381 tgtaggaagc ggcacctttg tttccagtac tcgagtacaa gaaaagatgc gagggacaac
     4441 cagttttact gaaattgtca aatcccaagg taaagttccc tctagccagc tcatttccta
     4501 caaaaaaacc attcccaatg agcaagaagt tgccaagcta ggaatttttc caacggacaa
     4561 tattatccga atggagcggg tccgctatgc cgaccaagtt cccctagttt atgaagttgc
     4621 ttctattcct gaaaaattca ttaaggactt taaaaaagaa gaaatcacca gtcatttctt
     4681 ccaaaccttg caaaaatatg gctatcgtat cggcaaatct caacagacca tctatgctcg
     4741 ccttgctaaa gaaaagattg cccactattt ggaagttgaa aaaggacatg ctattcttgg
     4801 attgacccag gtttcctacc tagaagatgg tactgctttt gaatacgtaa aaagtcaata
     4861 tgtaggcgaa cgctttgaat tttatcttga aaataattag attaccacgc aaaaactctc
     4921 tgtcacagac aaagagtctt ttttattttt ctaagaataa atctttcaaa gaaatcaaag
     4981 ttacagcaaa caatatcatt gccataccaa taaaatcaat gggataaaat tgttcattca
     5041 ttattaagaa ggcaaagaaa accgccgata ttggctcaat tgaagccaac aagcttgact
     5101 tgaccggtcc tatcagactg gctcctttaa ggaaagctgt ataggcaaag acagtcccga
     5161 taaggataat gcctgcaaac gcaaggagaa aatcaagact agtcgggata tcggcctgta
     5221 gaatccctgt aaaaggaagg gcgaccaaac ctgctatgac cattcccaca ccaatgacca
     5281 agctgctccc ccacttttta atcaaggcta tgggtaaaat gatatacaaa gcataagtca
     5341 aggcagaaaa gagaccccag aacagaccag caggtgtcat ggataactgg tccaactgcc
     5401 catgtgttgc gatcaggaag gttcctccga tggcgaatat gatggaaact atctctccca
     5461 gtgtcggtgc caccctatcc ttgatacagc tataaattaa aattccgaca ggacaaacat
     5521 actgaagcac cgtcgctgtt cccgcattgg tctcctgaat agcagacaga taggcgaatt
     5581 ggttgagaaa aagaccaatc agagcaaaaa taagaagaga cagcaaactc tttctatcct
     5641 ttaaaaagac cagtatttta tcctttgcag tagcataagc caagagcatg agaattccac
     5701 cagcgattaa aagacgcaag ttggtcaaga ccagagccga aattccgtgt gccattaggt
     5761 attggccact cgttcctgac aacccccaag caataccagc cacaactgtt agtaaagtcc
     5821 cttttaaact atttgacatg tctctcctta ctcttctttg cgaatcaagt tcatcacttg
     5881 attgcggtct aatatccatt gagctctctc gtccttttca taggtacgat tcgataggaa
     5941 aatagtggct tcttgcttct gacgattcca catgataaag gtacctgtat agcccgtatg
     6001 gtctagccaa tctccttcca aattccatgc taaagaacgt tccttgtcat ccaaaggaga
     6061 aaaattttga tttaagtctc ttgcaaaatc atctgctaaa tagtgttcta aaaagatttg
     6121 taaatccttt atagtcgaaa ataaaccagc actcccagca tgtctaccca ggagacgagc
     6181 cttgggatca tgcactatgc ctgcctctac acctctaact gttggaacag caagctcaac
     6241 tggcccaaac ttggtttccg tcattcccca aggtttccag acttgatcct ttaaaatcac
     6301 atccaaatct tgattaaaaa ttctttccaa aataaagccc aacagcaaaa aatggacatc
     6361 cgaataaaga aaggctggct gacttcgtct gttgagatga aacatcgctt cctttaattc
     6421 aggggctgtt aaaagatcac gattaggaat aaaaggatca aggtctgttg catgagtcaa
     6481 gagctggcga atagtgatgt ctggataatc actctcaggt aaaaaatcta ttaccagtct
     6541 atcaatatct aattgaccta tttcccacaa gaaggtacaa actgtgccaa ccccaacaac
     6601 cttgctgaca ctagctaggt cataaactag tcctgcctca gtctgcaagc catgctctgg
     6661 gtcactctgg cctaaataga actctgtcca ttgattgtcc ttaaaatacg caaaagaggc
     6721 tccgggataa atccctgcct cgatttgttc ttctattttt ttaataatct tggtccactt
     6781 catacttctt caaaccacaa ttcaacatta tttctatctt taccaaggaa gaatttttca
     6841 gacttaggaa taaaatattc aatagactca aacttctggc gaagacttgc tatgtctaat
     6901 tcattgacca agaacttgag catagataag tcccaggtaa ccgtattgtc cacagtcaaa
     6961 tcctgcccct gagctggaat aaaatcaagg gatgccccaa tttcagatga ttccaagaaa
     7021 ctttcgatat cagttgggag atgtaattcc atagaaatct caaatttact taaagaaatt
     7081 gatgccaaat ctgtttgaaa ttcaggcttt tctcctactt ctactagact tgctctgtca
     7141 tcttccgcat gaatcaaaat caaatcatct tctggtgaga aaatttcaaa agcgtagcca
     7201 ttttgacctt tatataatcg atgaatcgaa tctgttttag ataagattcc ttcaatttct
     7261 aagggatttt ccaccttgac aatcaatcta gctagttttt ttcttccctc taccttacga
     7321 gtacgcatac tgggagcttc ttctaaaacc agcttttcaa gacccgtttg gtcacctagt
     7381 gacagaaagg ccgattcttc taacaaggcc ttcattccaa gggtttcaat ataaaatgtt
     7441 tcatttaatt ttctattatt agcttttaaa g
//
LOCUS       DAVKAO010000078         7182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKAO010000078 DAVKAO010000000
VERSION     DAVKAO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.078"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            60..1730
                     /locus_tag="VWX16_001777"
     CDS             60..1730
                     /locus_tag="VWX16_001777"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9159081.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2059..3234
                     /gene="mutY"
                     /locus_tag="VWX16_001778"
     CDS             2059..3234
                     /gene="mutY"
                     /locus_tag="VWX16_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9159082.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            3289..3993
                     /locus_tag="VWX16_001779"
     CDS             3289..3993
                     /locus_tag="VWX16_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9159083.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            3986..5335
                     /gene="vicK"
                     /locus_tag="VWX16_001780"
     CDS             3986..5335
                     /gene="vicK"
                     /locus_tag="VWX16_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9159084.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            5337..6146
                     /locus_tag="VWX16_001781"
     CDS             5337..6146
                     /locus_tag="VWX16_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9159085.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            6237..6479
                     /locus_tag="VWX16_001782"
     CDS             6237..6479
                     /locus_tag="VWX16_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9159086.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            6481..6735
                     /locus_tag="VWX16_001783"
     CDS             6481..6735
                     /locus_tag="VWX16_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9159087.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            6782..>7182
                     /locus_tag="VWX16_001784"
     CDS             6782..>7182
                     /locus_tag="VWX16_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEU9159088.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGL"
BASE COUNT         2153 a         1266 c         1623 g         2140 t
ORIGIN      
        1 ttaaaaatct tgttttgtga tataatgaat tatcatataa gaggttagag gaattttgaa
       61 tgaaaacaga tattgaaatc gcacagagta ttgagttgaa gccaattgtt gatgttgtag
      121 agaaacttgg tatttcttac gacgatttgg agttgtacgg aaagtacaag gctaaactca
      181 gctttgataa aattcgtgca gttgagagca atccagtcgg taaattgatc ttggttactg
      241 ccatcaaccc aacacctgca ggtgaaggaa agtcgacgct taccattggt cttgcggatg
      301 ccttgaacaa gattggcaag aaaaccatga ttgctatccg cgaaccgtct cttggtccag
      361 tcatggggat caagggtggt gctgctggtg gtgggtatgc acaagttctg ccaatggaag
      421 acatcaacct ccactttact ggagatatgc atgctattac aactgccaac aatgcccttt
      481 ctgccttgat tgacaaccac ttgcaccaag ggaatgagct gggaattgat caacgtcgta
      541 tcctctggaa acgcgttgtg gacttgaacg accgtgcgct ccgccatgtg actgttggtc
      601 ttggtggtcc tctaaacggt attccacgtg aggatggttt tgatattaca gttgcttcag
      661 aaatcatggc aattctttgc ttggcaacgg acatcgagga cttgaaacgt cgtttggcga
      721 atatcgttat tggttatcgc tatgaccgta cgcctgtttc tgtaggtgat ttgcaggttg
      781 agggtgcctt ggctttgatt ttgaaggatg ctattaagcc aaacttggtt cagacaattt
      841 acggtacacc tgcctttgta cacggtggtc catttgccaa tatcgctcat ggctgtaact
      901 ctgttttggc gacgacaaca gcccttcact tggctgatta cactgttact gaagctggtt
      961 ttggtgcgga ccttggtgct gagaaattcc ttgatatcaa gacaccaaac ttgccaacat
     1021 ctccagatgc agttgttatt gtcgcaaccc ttcgtgccct taagatgaat ggtggtgtgg
     1081 ctaaagacgc tctgactgaa gaaaatgtag aggcagttcg tgcaggtttt gctaacttga
     1141 aacgccacgt tgaaaatatc cgtaagttcg gtattccagc agttgtagct attaacgaat
     1201 ttgtatctga tacagaagct gaaattgcag ccttaaaaga actctgtgcc tcaatcgatg
     1261 taccagttga attggctagt gtctgggctg atggagcaga aggtggagta gcacttgccg
     1321 aaacagttgt taagacaatt gctgaaaatc cagctaacta taaacgtttg tacgataatg
     1381 acctttctgt ccaagaaaag attgaaaaaa ttgtcactga aatctatcgt ggtagcaaag
     1441 tgaactttga gaagaaagct caaacacaaa ttgctcaaat cgttcaaaac ggttgggaca
     1501 aattgccaat ttgtatggct aaaactcaat acagtttctc agacaatcca aatgcgcttg
     1561 gagcaccaga aaactttgaa attaccattc gtgaattggt accaaaatta ggtgcaggct
     1621 tcatcgttgc cttaactggt gatgttatga ccatgccagg tcttccaaaa cgtccagcag
     1681 ctctcaacat ggatgttgaa agcgatggaa ctgtactagg cttgttctag tggattgaaa
     1741 ttgactttaa gtgagtttgg aaataatatc caagctcatt ttttctcaga ctgcaagcag
     1801 ttcatcaata agatttatac tcttcgaaaa tcttttcaaa ctacgtcagc ttcaccttgc
     1861 cgtaggtaat atgatactga ctctgtcagt tctatccaca acctcaaaac agtgttttga
     1921 gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtata agtagataga
     1981 aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag gtgaaacctt
     2041 ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat gtggccggag
     2101 gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa caaaagagat
     2161 ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat catgcttcag
     2221 cagaccaggg tggatacagt tatcccttac tacgaaagat ttttggactg gtttccaact
     2281 gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga gggcttgggt
     2341 tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac tgactttggt
     2401 ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg accttacaca
     2461 gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga tggtaatgtc
     2521 atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc aagtaatcga
     2581 aaaatttttc aggcaatgat ggagatcttg attaatccag atcgtccggg tgattttaat
     2641 caagccttga tggacttagg gtctgatatt gagtctcctg taaatcccag acctgaagaa
     2701 agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg ttatccaatt
     2761 aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt gaaaaattct
     2821 caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg tttttggcat
     2881 ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct ctttcatcag
     2941 gttgcggaag aaagtgtgaa ctctggcccc agtccagaag agagtttcca gcaggactat
     3001 gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt ttttagtcat
     3061 cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga tttttcagat
     3121 agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc caaaccccaa
     3181 caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga ctagctattg
     3241 tgtcttctga ctattttttg gtataatagt agagaaaaag gtgaacatat gaaaaaaata
     3301 ctaattgtag atgatgagaa accaatctcg gatattatca agtttaatat gaccaaggaa
     3361 ggttacgaag ttgtaactgc ttttaatggt cgtgaagcgc tagagcaatt tgaagcagag
     3421 caaccagata ttattattct ggatttgatg cttccagaaa ttgatggttt agaagttgct
     3481 aagaccattc gtaagacaag cagtgtgccc attcttatgc tttcagccaa agatagtgaa
     3541 tttgataagg ttatcggttt ggaacttggg gcagatgact atgtaacgaa acccttctcc
     3601 aatcgtgagt tgcaggcgcg tgttaaagct cttctgcgtc gttctcaacc tatgccagta
     3661 gatggtcagg aagcagatag taaacctcaa cctatccaaa ttggggattt agaaattgtt
     3721 ccagacgcct acgtggctaa aaaatatggc gaagaactag acttaaccca tcgtgaattt
     3781 gagcttttgt atcatttagc atcgcataca ggtcaagtca tcacgcgcga acacttgctt
     3841 gagactgtct ggggttatga ctattttggt gatgtccgta cagttgatgt gactgtacga
     3901 cgtctgcgtg agaagattga agatacgccc agccgaccag agtatatctt gacgcgccgt
     3961 ggtgtagggt attacatgag aaataatgct tgatttactg aaacaaacca tttttaccag
     4021 agattttatc tttatcctga ttttgttagg tttcatcctt gttgtgaccc tcttattact
     4081 ggaaaataga cgtgataata ttcagttgaa gcaaatcaat caaaaggtta aagatttgat
     4141 tgcaggagat tattccaagg ttcttgatat gcaaggtggg tctgaaatca ccaatattac
     4201 caataatttg aatgacttgt cggaggttat tcgtctcact caggaaaatc tagaacaaga
     4261 gagtaagagg ctaaatagta ttctgtttta tatgacagat ggggttcttg cgaccaaccg
     4321 tcggggtcag attatcatga ttaacgatac agccaagaag caactggggt tggttaagga
     4381 agatgttctg aatagaagca ttttggaatt gctcaagata gaagaaaact atgaattgcg
     4441 tgatttgatt acccaaagtc cagaattgtt gctagattcc caagatatca atggtgaata
     4501 tttgaacctt cgagttcgct ttgccttgat acgtcgagag tctggcttta tttcaggttt
     4561 ggtggctgtt ttgcatgata cgacggagca ggagaaggaa gaacgcgaac gaagactctt
     4621 tgtttccaat gttagccatg agttacggac tcctctgact agcgtaaaat cctatcttga
     4681 agccttggat gagggggctt tgtgtgaaac tgtagcacca gactttatca aggtttctct
     4741 tgatgagacc aaccgtatga tgcgcatggt gacggatctc ctccatcttt cacgtattga
     4801 taatgctacc agtcacctag atgtggaact gattaacttc actgctttta ttacctttat
     4861 cctcaatcgt tttgacaaga tgaaaggaca ggaaaaggag aaaaaatatg agttggtgag
     4921 agattatccc atcaattcta tctggatgga aattgataca gataagatga cgcaggttgt
     4981 cgacaatatt ttaaataatg ctattaagta ttcgccagat gggggtaaaa tcactgtcag
     5041 aatgaagaca actgaagacc agatgatttt atccatttct gaccacggtt tggggattcc
     5101 taagcaggat ttaccacgta tctttgaccg tttctatcgt gtggatcgtg ctagaagtcg
     5161 tgcacaaggt ggtacaggtc taggactgtc tatcgctaaa gaaattatta aacaacataa
     5221 gggctttatt tgggccaaga gtgaatacgg caagggttca acctttacca ttgtgctccc
     5281 ttatgataag gatgcagtga aagaagaagt atgggaggat gaagtagaag actagaatga
     5341 gtgaaatagg ctttaaatac agtattttag cgtcgggttc cagtggaaat tctttttatc
     5401 tggaaacctc aaaaaagaag cttttagtgg atgcaggctt gtctggcaag aaaattacca
     5461 gtctgctagc tgaaattaac cgcaagccag aagacctgga tgccatcttg attacccatg
     5521 agcattcaga tcatatccat ggagtaggcg ttttggctcg caagtatggt atggatcttt
     5581 atgccaatga aaagacctgg caagctatgg aaaatagtaa atatcttggc aaggtggatt
     5641 cttcgcaaaa gcatattttt gaaatgggta aaaccaaaac ctttggagat atcgacatcg
     5701 agagttttgg tgtaagccat gatgcagtcg caccgcagtt ctatcgcttt atgaaggatg
     5761 ataagagttt tgtccttttg acagatacag gttatgtcag tgaccgtatg gcgggcattg
     5821 tcgaaaatgc ggatggctat cttatcgagg ccaaccatga tgtagagatt ttgcgatcag
     5881 gttcttacgc ttggcgactc aaacaacgaa tcctatctga ccttggtcac ctttctaacg
     5941 aggacggtgc tgaagctatg attcggacgc taggaaatcg tactaagaag atttaccttg
     6001 ggcatttatc taaggaaaac aatatcaagg aactggctca tatgaccatg gtcaatcagc
     6061 tggctcaagc tgatctggga gtcggagtag actttaaggt ttatgatacc tcaccagata
     6121 ccgcaacacc attgacagag atatagaaag aacgctgaga aggtgttctt tttatattga
     6181 ctgaacacct aaaaagtaat acaatggtgt taccattaaa aaagggagca caaaagatga
     6241 ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca atggctaagt
     6301 ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc ctagaagatg
     6361 aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac ttggaaaaag
     6421 gagttgaacc cattacttgg gaagaaatga tgcatgattt aggcttgaag gatgaataat
     6481 ttgtataaat tagttccaac aagacgtttc atcaagcaat tgaaaaaatt ggaccgttat
     6541 acgcagaagc taattacaaa ctatttacaa atcaatgttt tggaagaccc aagacgacac
     6601 ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg taattatcga
     6661 gttatcgtac aaattgtaga tgatgaatta gttgttgcta ctctagaagt tggtcatcgg
     6721 agagatattt attgaattaa agctgagtaa taacaatata gaaagtaagg agagtaaaaa
     6781 tttgaagaaa aaatggtttt ttgcagatta ttatgataca acaattattt tacttgcttt
     6841 aatctctgtt attctggtat tacttggatt tgctgagatg attgatttgg acaatccgcc
     6901 ttatagtatt attgatttag taatctgggg tgtttttgta attgattata gttggcgctt
     6961 ttttattact aaaagaaaat ggcgctttat tcttgaaaat atttttgatt tattggctat
     7021 tctcccttta aatgctattt tcacagtttt tcgattaggg cgtatctttc gtttagttaa
     7081 actaacaaaa ttactgaaac tgactcgttt acttagaata attggactta caggtaaatt
     7141 agaaagaaaa attagtagat tcttacggac aaatggcctg at
//
LOCUS       DAVKAO010000079         6719 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKAO010000079 DAVKAO010000000
VERSION     DAVKAO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.079"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            136..999
                     /locus_tag="VWX16_001785"
     CDS             136..999
                     /locus_tag="VWX16_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9159089.1"
                     /translation="MNILAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1382..2002
                     /locus_tag="VWX16_001786"
     CDS             1382..2002
                     /locus_tag="VWX16_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159090.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2088..2531
                     /gene="tsaE"
                     /locus_tag="VWX16_001787"
     CDS             2088..2531
                     /gene="tsaE"
                     /locus_tag="VWX16_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9159091.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2521..3039
                     /locus_tag="VWX16_001788"
     CDS             2521..3039
                     /locus_tag="VWX16_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9159092.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            3047..4063
                     /locus_tag="VWX16_001789"
     CDS             3047..4063
                     /locus_tag="VWX16_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9159093.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4148..5404
                     /locus_tag="VWX16_001790"
     CDS             4148..5404
                     /locus_tag="VWX16_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9159094.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5459..6625
                     /gene="recA"
                     /locus_tag="VWX16_001791"
     CDS             5459..6625
                     /gene="recA"
                     /locus_tag="VWX16_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9159095.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         2136 a         1089 c         1505 g         1989 t
ORIGIN      
        1 cggtcaagca tttttatgta atttttgact ttttttagtt tattaccata aagaattatt
       61 tattccttta acttgctatt ttgaactaaa aattttataa tgcaattaag caaataggaa
      121 ggtttattat ctttaatgaa tatactcgca gagaaattca gattgaaaag aaaagagttg
      181 agactctccc aacaaactct tgcagaagga atttgtgaac aaagtcagat tagtaaaatt
      241 gagagagggc atttcattcc ctccgcagac cttttgttca aactctctca acgacttgaa
      301 gtaccattag attatttttt taatgaacaa attgaaatta aatctaacct ctctaatttc
      361 aagcaattgt ctgctcgact attagacgat agaaattatg acgatttgga atatatttat
      421 agaatagaga ttgaacgaag tacttttcta acactagaag accgaactta ccttgaatgg
      481 attaaagcta ttattgactt ctatcaatat gacagtaagt gtgaggcaat ttcttcattg
      541 gaaaatatat tattaaaagt ctcttcaaat actctgattt atttaaaggc gttgaatact
      601 ctatctaatt tctattcctt agtgggtcgt gaacaagaat atgaggcaaa ctactctcat
      661 ttaatagagt tgtatcagac aaaaaatttg gaccatcaag agtttttatt tggctacatc
      721 agagttcgtt acaactacgc tcactaccta gtatcaaagg aaaaatataa tgaagccatc
      781 caagaagctc ttgaaacgat tgaactctgt aaacaaagac agacaagcta ccaattggct
      841 cctctactta ttcttgtagg aaatgctgga gccaaatttc tagacagaga acaagtcaaa
      901 aattattata tagaagcaag agagttatgt aagatttata acaatccttt aatgttgatg
      961 aagatagaaa attatttgaa ggaactagat actgtttagt taatcttgac attatagttt
     1021 ttctgaaacg ttaaataacc tgtaaggctg atagttagat taatactatc agccttataa
     1081 ttatgtgact ctgagtccgt ctcgctccgt gaggtgcgag acaaaacaac cacccgtttg
     1141 acggattttc atgatttcaa atttatattc ttcgaaaatc tcttcaaacc acgtcagctc
     1201 tatctgcaat ctcaaaacag tgttttgagc aacctgtggc tagctaccta gtttgctctt
     1261 tgattttcat tgagtattag tctctagtgt ttgtttagcg tcttaaagag acatttgaga
     1321 agattagaaa cagttctctc ttttctccta gtataatgat tggtaggaag ggagagagaa
     1381 gatgaaatca atgagaatct tatttttgtt agctttaatt caaatcagtt tgagtagctg
     1441 tttcctatgg aaggaatgca tcttgtcctt taaacaaagt acagcttttt tcatcggaag
     1501 catggttttc gtttcaggaa tctgtgctgg agtaaattat ctttatactc gtaagcaaga
     1561 agtccatagt gtcctagcca gtaagaagtc ggtgaagctt ttttacagta tgttactctt
     1621 aattaatttg ttaggagctg ttcttgtttt gtcagataac ttgttcatca aaaatacgct
     1681 gcagcaagaa ttagttgact ttttattgcc atccttcttt ttcctatttg ggctagattt
     1741 gctgattttt ttacccttga aaaaatacgt gcgcgatttt cttgctatgc tggacagaaa
     1801 aaagacagtg ttggtgacta ttttagcaac acttcttttc ttaagaaatc caatgaccat
     1861 tgtctcactt ctgatttata ttggactggg cttgtttttt gcagcctatc ttgtcccaaa
     1921 ttcggttaag aaggaagttt ccttttatgg tcatattttc cgagatcttg tattggtcat
     1981 tgttacgctc attttctttt agagaaagcc tgttttttat ggatgttatg atgataagtt
     2041 tagtaggaaa atagacacaa aagaaaagtt tttggtataa taagagtatg tacacaaaaa
     2101 atgaagaaga gttgcaagcc ttaggggagc gtttgggcca tctattagca aagaatgatg
     2161 ttttaatctt aactggagaa ctgggtgcag gtaaaacgac ctttactaaa ggacttgcaa
     2221 aaggattaca gatttctcaa atgattaaaa gtcccaccta tactatcgtg agagagtatg
     2281 aaggtcgact tccactttat cacctagatg tttatcgtat tgaaggagat gctgattcta
     2341 tcgacttgga tgagtttatc tttggtggcg gcgtgactgt tattgagtgg ggaaatctct
     2401 taggagatgc cttgccagat gcttatttag aattggaaat tctaaaagaa gcagatggac
     2461 gccgtttaaa ttttcaggca aagggtttgc gtgctgagaa attgttagag gagcttcaat
     2521 atggagtatg aattgctcat tagggaagca gagcccaaag atgcagctga attagtggcc
     2581 tttttaaatc gtgtgagttt ggagacagac tttaccagcc tagacggaga tggtattctc
     2641 ttgactagtg aggagatgga aatattcctc aacaagcaag ctagttcgga caatcagata
     2701 accttacttg cattcttaaa tggcaaaatt gctggtattg taaatattac agctgatcag
     2761 cgtaagagag tccgtcatat tggagatctc ttcattgtga ttggaaaaag atattggaat
     2821 aatggcttgg gaagtttgtt gctagaagaa gcgatagagt gggcacaagc aagtggcatt
     2881 ctgcgtcgtc tccaactgac tgtccaaact cgtaatcaag cagcagtcca tctttatcaa
     2941 aagcatggct ttgtcattga aggtagccaa gagcgtgggg catatataga agaagggaaa
     3001 tttatcgatg tttacttgat gggtaaactg ataggttagt agaaatatgg ttaaaaaaat
     3061 tattggaatg gtgctagctt tactttctgt aactgtagta ggagtaggtg tttttgctta
     3121 tactatttat caacaaggga cagaaacctt agctaaaacc tataaaaaaa tcggtgaaga
     3181 aaccaaggtt attgaagcga ctgaacctct aaccattctg ttaatgggag tggacaccgg
     3241 aaatgttgaa cgaactgaaa cttgggtcgg tagaagtgat agcatgatct tgatgacagt
     3301 gaatcctaaa acgaaaaaaa caacaatgat gagtttagag cgggatattc tgacgcgcat
     3361 tgaatcaggg aatggtcagg ctcatgaagc gaaactgaac tcagcatatg cagatggtgg
     3421 agcagagctt gctatagaaa ccattcaaaa aatgatgaat atccatattg atcgctatgt
     3481 gatggtcaat atgagagggt tgcaaaaatt agtggatgca gtaggaggta ttacagtcaa
     3541 taatatccta ggtttcccaa tttctatcag tgaccaagaa gaatttaata ccatttctat
     3601 cggtgttggg gagcaacata ttgggggaga agaagcccta gtctatgcac gaatgcgtta
     3661 ccaagatcct gagggggatt atggtcgtca aaaacgtcaa cgtgaagtta ttcaaaaagt
     3721 catggaaaaa gctctcagtt taaatagcat tggtcattat caagagattc taaaagcttt
     3781 gagtgacaat atgcagacca atattgattt gtctgcaaaa agtatcccta acttgctagg
     3841 ctataaagat tcatttaaaa ccattgaaac tcagcagttg cagggtgaag gagagatact
     3901 tcaaggtgtt tcttaccaga ttgtttcgag agcacatatg ttggaaatgc aaaatatact
     3961 ccgacgttct ttgggacaag aagaagttac tcagcttgaa accaatgcgg ttttatttga
     4021 agatttattt ggcagagcac ctgttggtga tgaagataat taacttttga tacaaataaa
     4081 aaaatcaatc gtaggaaaat tcctgcgatt ttttcaaaaa aatacgaata gataggtagg
     4141 aggaaacatg aaagcagaaa tcattgctgt tggaacagag attttgacag gacagattgt
     4201 caacaccaat gcccagtttt tgtcggaaaa actagctgag attggggtag atgtatattt
     4261 tcagacggct gtaggagaca atgaagttcg tctcttgtct ttgcttgaga ttgccagtca
     4321 acgtagcagt ctggtgattt tgacaggcgg tttggggcca actgaggacg acctaaccaa
     4381 acaaacccta gctaaatttt tagggaaagc attagtcttt gatcctcagg ctcaggagaa
     4441 gttggatatc ttttttgccc tgcgaccaga ctatgcccga acaccgaata acgaaagaca
     4501 agctcaaatt gtagaaggag cgattccact gccaaacgaa acaggactgg ctgttggagg
     4561 aaaattagaa gtagacggag tgacctatgt cgtccttcca ggtccgccaa gtgaattgaa
     4621 acccatggtc ttaaaccaac ttctacccaa gttgatgaca gggagcaagc tgtattcccg
     4681 agttcttcgt ttctttggga ttggcgagag ccagctggtt acgattttgg ctgatttaat
     4741 tgataatcag atcgatccta ccttggcccc ttatgccaag acaggagaag tcactctacg
     4801 tctgtcaaca aaggctagca gtcaagaaga ggcgaatcaa gcgctggata tcttggaaaa
     4861 tcaaatcttg gactgccaga ctttcgaagg aatttcttta cgagactttt gctatggtta
     4921 tggggaagaa actagtttag ccagcattgt ggtagaagaa ctgaaaaggc aagggaaaac
     4981 catcgcggct gcagagagtt tgacggcagg tcttttccaa gctaccgtgg cgaatttttc
     5041 tggagtttca agtatattta agggtggttt tgtgacctat agcttggagg aaaaatcaag
     5101 gatgttggat attcctgcca agaatttgga agaacatggt gtggtgtctg aatttacagc
     5161 tcagaagatg gctgagcagg cacgaagcaa gacccagtct gattttggaa ttagtttgac
     5221 cggagtggca ggaccagata gcctagaagg acacccagtt gggacagtct tcataggctt
     5281 ggcgcaagag caaggaactg aggttatcaa ggttaatatt ggaggcagaa gccgagcaga
     5341 tgtacgtcat attgcggtta tgcatgcctt taacctagtt cgcaaggctt tattaagtga
     5401 ctaacttttg atataatagt agataggtct gaggatcatt agaatgtagg agaatagaat
     5461 ggcgaaaaaa ccaaaaaaat tagaagaaat ttcaaaaaaa tttggggcag aacgtgaaaa
     5521 ggccttgaat gacgctctta aattgattga gaaagacttt ggtaaaggat caatcatgcg
     5581 tttgggtgaa cgtgcggagc aaaaggtgca agtgatgagc tcaggttctt tagctcttga
     5641 cattgccctt ggctcaggtg gttatcctaa gggacgtatc atcgaaatct atggcccaga
     5701 gtcatctggt aagacaacgg ttgcccttca tgcagttgca caagcgcaaa aagaaggtgg
     5761 gattgctgcc tttatcgatg cggaacatgc ccttgatcca gcttatgctg cggcccttgg
     5821 tgtcaatatt gacgaattgc tcttgtctca accagactca ggagagcaag gtcttgaaat
     5881 tgcgggaaaa ttgattgact caggtgcagt tgatcttgtc gtagtcgact cagttgctgc
     5941 ccttgttcct cgtgcggaaa ttgatggaga tatcggagat agccatgttg gtttgcaggc
     6001 tcgtatgatg agccaggcca tgcgtaaact tggcgcctct atcaataaaa ccaaaacaat
     6061 tgccattttt atcaaccaat tgcgtgaaaa agttggagtg atgtttggaa atccagaaac
     6121 aacaccgggc ggacgtgctt tgaaattcta tgcttcagtc cgcttggatg ttcgtggtaa
     6181 tacacaaatt aagggaactg gtgaccaaaa agaaaccaat gtcggtaaag aaactaagat
     6241 taaggttgta aaaaataagg tagctccacc gtttaaggaa gccgtagttg aaattatgta
     6301 cggagaagga atttctaaga ctggtgagct tttgaagatt gcaagcgatt tggatattat
     6361 caaaaaagca ggggcttggt attcttacaa agatgaaaaa attgggcaag gttctgagaa
     6421 tgctaagaaa tacttggcag agcacccaga aatctttgat gaaattgata agcaagtccg
     6481 ttctaaattt ggcttgattg atggagaaga agtttcagaa caagatactg aaaacaaaaa
     6541 agatgagcca aagaaagaag aagcagtgaa tgaagaagtt acgcttgact taggcgatga
     6601 acttgaaatc gaaattgaag aataagctgt taaagcagtg gaaaaatccg ctactttttc
     6661 gatttttgat tcaagttttt agattatata tagtagcttg aaataagata tgaacaact
//
LOCUS       DAVKAO010000080         6619 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKAO010000080 DAVKAO010000000
VERSION     DAVKAO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.080"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..207
                     /locus_tag="VWX16_001792"
     CDS             <1..207
                     /locus_tag="VWX16_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9159096.1"
                     /translation="MDNVRFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIK
                     KHLKKVLPSCNTFYEALLSCSCFN"
     gene            268..1230
                     /locus_tag="VWX16_001793"
     CDS             268..1230
                     /locus_tag="VWX16_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9159097.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLSEDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAEASKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFMKAVSYFILEFKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEVIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1410..2417
                     /gene="gap"
                     /locus_tag="VWX16_001794"
     CDS             1410..2417
                     /gene="gap"
                     /locus_tag="VWX16_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9159098.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2485..3321)
                     /locus_tag="VWX16_001795"
     CDS             complement(2485..3321)
                     /locus_tag="VWX16_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9159099.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3447..5642
                     /locus_tag="VWX16_001796"
     CDS             3447..5642
                     /locus_tag="VWX16_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9159100.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQVVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5695..5847
                     /gene="rpmG"
                     /locus_tag="VWX16_001797"
     CDS             5695..5847
                     /gene="rpmG"
                     /locus_tag="VWX16_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9159101.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5857..6033
                     /gene="secE"
                     /locus_tag="VWX16_001798"
     CDS             5857..6033
                     /gene="secE"
                     /locus_tag="VWX16_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9159102.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6088..>6619
                     /gene="nusG"
                     /locus_tag="VWX16_001799"
     CDS             6088..>6619
                     /gene="nusG"
                     /locus_tag="VWX16_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9159103.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2051 a         1159 c         1439 g         1970 t
ORIGIN      
        1 atggataatg taagattcca tagaatgggg aagctagaac ttttatgcga agagtttggg
       61 cataaactgt tacctcttcc tccctactcg cctgagtaca atcctattga gaaaacatgg
      121 gctcatatca aaaagcacct caaaaaggta ttaccaagtt gcaatacctt ttacgaggct
      181 cttttgtctt gttcttgttt caattgacta tatatcacgg atacagttgg gaaagaagtt
      241 attaattatt tggagagtcc ttttgggatg aacatgtcaa atattactat agatgggcaa
      301 gaaatggatc tgagtcaaac gatgccttct tatgctcaag tagtgagtct ttctgaggat
      361 acaagcatct ctgttagtag taatgagacg gacaaagtgt tggctggatc cctctatact
      421 gatacaaatg aacagggatt aacgattcca agcagtttac taaaaaattg gaatgaacag
      481 acaggaaaaa atttgacagc taatgatctt attggcaaat cagtctcagc cagcattgta
      541 gaaagtgctg ccgaagctag taagattgct caatttcaaa cgaagattgt acgtgtaatc
      601 aatgatgaag atgacatgga ggacagcaac agtttcatgc tgtctcatca aatggaaacg
      661 attttgaaag aggctggatt tatgaaagct gtatcttatt ttatcttgga attcaaagat
      721 ccatcacaga caaaagtagt aacagaagaa ttacagaaaa ataagaagta tactgtgctt
      781 tctcaacaga gagttcttga tattgtgatt acctttattc gtgttattca gggattattg
      841 attgtgcttt catcacaagc tattgtggta gcagcggtta tgattggtat tattatttac
      901 atcaatatca tgcaacgttc caaggaaata ggtgtcatga aggcagttgg ttatcagaat
      961 cgtgatgtca aaggaatttt tatttacgag gttatctgga ttgtaggcat cgccttgctg
     1021 ctggcatttt tggttgcaca aggggtggga agtttggcga atgcgattgt aagtcacttt
     1081 tacccatcca tcactaaggt ttttgaatta aatcttttat ctgttttagg aactctagtt
     1141 ttcgctctat tacttggtta tgtctcagcc tacttcccgg cgcgtaagat tagtaaaatg
     1201 gaccctgtag aatcgctacg ctatgaatag tcaagggtgt tactagatta tcgtccctaa
     1261 aaaagaaact aacagagaaa aacaatgccc gtacttgcaa ttcaactcaa atagttatat
     1321 aataggtttg ttgaaaggtg atttgtagtg attcaagtta ctcttttcac aataaaaatt
     1381 tcatgatttt cataaggagg aaatcactaa tggtagttaa agttggtatt aacggtttcg
     1441 gacgtatcgg tcgtcttgct ttccgtcgta tccaaaacgt agaaggtgtt gaagttacac
     1501 gcatcaacga ccttacagat ccagttatgc ttgcacactt gttgaaatac gacacaactc
     1561 aaggtcgttt cgacggtact gttgaagtta aagaaggtgg atttgaagtt aacggtaaat
     1621 tcatcaaagt ttctgctgaa cgtgatccag aacaaatcga ctgggctact gacggtgtag
     1681 aaatcgttct tgaagctact ggtttctttg ctaagaaaga agcagctgaa aaacacctta
     1741 aaggtggagc taaaaaagtt gttatcactg ctcctggtgg aaacgacgtt aaaacagttg
     1801 tattcaacac taaccacgac gttcttgacg gtactgaaac agttatctca ggtgcttcat
     1861 gtactacaaa ctgcttggct ccaatggcta aagctcttca agacaacttt ggtgttgttg
     1921 aaggattgat gactactatc cacgcttaca ctggtgacca aatgatcctt gacggaccac
     1981 accgtggtgg tgaccttcgc cgtgctcgcg ctggtgctgc aaacatcgtt cctaactcaa
     2041 ctggtgctgc aaaagctatc ggtcttgtaa tcccagaatt gaatggtaaa cttgatggat
     2101 ctgcacaacg cgttccaact ccaactggat cagttactga attggtagca gttcttgaaa
     2161 agaacgttac tgttgatgaa gtgaacgcag ctatgaaagc agcttcaaac gaatcatacg
     2221 gttacacaga agatccaatc gtatcttcag atatcgtagg tatgtcttac ggttcattgt
     2281 ttgacgcaac tcaaactaaa gttcttgacg ttgacggtaa acaattggtt aaagttgtat
     2341 catggtacga caacgaaatg tcatacactg cacaacttgt tcgtactctt gaatacttcg
     2401 caaagattgc taaataattc ttgagttgat agaaagcaag gctttgtggt cttgcttttt
     2461 tatatggaaa aatggatgac acgatcatcc attctttttt aattcttttt caaatgtatt
     2521 tgaaagggta gtgaaagtta gcctctctaa agtaagtggg tgggtaaagg aaagtcggaa
     2581 ggcatgaagc ataagccggc ttgtctttga tttactatta tagagagggt ctcccaggat
     2641 aggaagatta tgatgcaaaa ggtgcacacg aatctgatgg gttcgccctg tctttagctt
     2701 gcaatgagcc aaggaagtct tgtttgagaa ttgctttaat ctgcttacat gcgtttcagc
     2761 atattgccca ttttttgcat caactattct ttttctacga tcatggcgat cacgtccaat
     2821 tttgtctctg aaaacaagtt cttttctgtt gatatttcca tcaactagag cccaatattc
     2881 tctagaaatc tcttttttct ccaataagcg attgagaatg ggcaggataa aaggattttt
     2941 ggcaaagaga actaagccac tggtttccat gtccagacga tgaacgacat agcaggtttg
     3001 gccaacatag gtactgacat ggttaagaag ggcaatttcg tttggttgat taccatgcgt
     3061 tttcatcccc tctggtttgt ttacaataat caagtgttga tcttgataaa cttcctgcac
     3121 taagtctggg ttgccccaag ggatcgtctt ttggggataa tcttcctcgt caaaagtcaa
     3181 ctggcaaaca tctccaggat ttacgatttc gttccagtgg acttcttctt gatttatcaa
     3241 aatatgtttc ttgattctca aaaaatgacg gatttttcta gggatgagga gttgttcctc
     3301 aagtaattgc tttaccgtca tttgaggtag agaggcgggt aatgtaaatg tgaattgcat
     3361 acagatattg taacaaaaaa agccctattt ggataggaaa tagctaaatt cttgtcttcc
     3421 tatgatgaag atgataaaat aaacgcatga aattagataa attatttgag aaatttcttt
     3481 ctctttttaa aaaagaaaca agtgaattag aggactctga ttctactatc ttacgtcgct
     3541 ctcgtagtga tcgaaaaaaa ttagcccaag taggtccgat tcgaaaattc tggcgtcgtt
     3601 atcatctaac aaagattatc cttatactag gtttgagtgc aggcttgcta gttggaatct
     3661 atttgtttgc tgtagccaag tcaaccaatg tcaatgattt gcaaaatgcc ttgaaaactc
     3721 ggactcttat ttttgaccgt gaagaaaaag aggctggtgc cttgtctggt caaaagggaa
     3781 cctatgttga gctgactgat atcagtaaaa acttgcagaa tgctgttatt gcgacagaag
     3841 accgttcttt ctataaaaat gacgggatta actatggccg tttcttcttg gctattgtca
     3901 ctgctggacg ttcaggtggt ggctctacca ttacccaaca gctggctaaa aacgcctatt
     3961 tatcacagga tcaaactgtt gagagaaaag cgaaagaatt tttccttgcc ttagaattaa
     4021 gcaaaaaata tagtaaggag caaattctaa ccatgtacct taacaacgct tattttggaa
     4081 atggtgtgtg gggtgtagaa gatgcgagta agaaatactt tggagtttct gcatcagaag
     4141 tgagtctgga tcaagctgcg actctggcag ggatgctcaa ggggccggaa ctgtataatc
     4201 ccttgaattc cgtagaagat tctactaatc ggcgcgatac tgtcttgcag aatatggttg
     4261 cagcaggata tattgataaa aaccaagaaa ccgaagctgc tgaagttgat atgacttcgc
     4321 aattgcacga taagtatgaa ggaaaaatct cagattaccg ttacccctct tattttgatg
     4381 cggtggttaa tgaagctgtt tccaagtata atctaacaga ggaagagatt gtcaataatg
     4441 gctaccgcat ttacacagag ctggaccaaa actaccaagc aaatatgcag gttgtttatg
     4501 aaaacacatc gctatttccg agggcagagg atggaacgtt tgctcaatca ggaagtgtag
     4561 ctctcgaacc gaaaacaggg ggagttcgtg gagttgtcgg tcaagttgct gacaatgata
     4621 aaactggatt ccggaatttc aactatgcaa cccaatcaaa gcgtagtcct ggttctacaa
     4681 ttaagccttt agttgtttat acgccagcag ttgaagcagg ctgggctttg aataagcagt
     4741 tggataacca taccatgcag tatgacagct ataaggttga taactatgca gggatcaaaa
     4801 cgagtcgaga agttcctatg tatcaagcct tggcagaatc gcttaatcta cctgctgttg
     4861 ccactgttaa tgatttgggt gtcgacaagg cttttgaggc aggcgaaaaa ttcggactca
     4921 acatggaaaa ggttgaccgt gttcttggtg tcgccttggg aagcggtgtt gaaaccaacc
     4981 ctcttcaaat ggctcaagca tacgccgcct ttgcaaatga aggtttaatg cctgaagctc
     5041 attttattag tagaattgaa aatgctagtg gacaagttat tgcgagtcat aaaaattcac
     5101 aaaaacgggt gattgataag tctgtagctg acaagatgac cagtatgatg ttggggactt
     5161 tcaccaacgg taccggtatt agttcatcgc ctgcagacta tgtcatggca gggaaaactg
     5221 gaacaactga agcagttttc aatccggagt acacaagtga ccagtgggta attggttata
     5281 ctccggatgt agtgattagc cactggcttg gctttccgac cactgatgaa aaccactatc
     5341 tagctggctc tacttcaaac ggtgcagctc atgtctttag aaacattgcc aataccattt
     5401 taccttatac gtcaggaagt acctttacgg ttgaaaatgc ttataagcaa aatggaattg
     5461 caccagccaa tacaaaaaga caagtacaaa ccaatgataa tagccagaca gatgataatt
     5521 tgtctgatat tcgagggcgt gcgcaaagtc tagtagatga ggctagccgg gctatctcag
     5581 atgcgaagat taaggaaaag gctcaaacaa tatgggattc gatagtcaat ctatttcgct
     5641 aagatgcttg tcaaagccta gctttcttgt tataatggat aagatggagg cgttatggca
     5701 ctaaaaaaag caagcctagc ttgtgcggtt tgtggttcga gaaactattc aatcaagatc
     5761 agcggaaacc ccaagcctac acgactagaa gtaaataaat tttgtaagca ttgtggcaag
     5821 tacactacac atagagaaac gagataggag agagcgatgc gttttattgg agatattttt
     5881 agacttctta aagacacaac gtggccaact cgcaaggaaa gctggagaga ttttcgttct
     5941 atcatggaat acacagcttt ctttgtagta attatttaca tttttgacca gttgattgtt
     6001 tcaggtttga ttcgatttat taacattttt tagaagatta gtggagttaa ttacactaga
     6061 aatcttctat ttatgaaagg aaatatcatg gatagttttg ataaaggatg gtttgtttta
     6121 caaacttatt ctggttatga aaataaggtg aaagaaaatc tattacaacg tgcacaaacc
     6181 tacaatatgt tggataatat tctacgcgtt gaaattccaa cacaaacagt gcaagttgaa
     6241 aaaaatggaa agagaaaaga agtagaagaa aatcgctttc caggttatgt tcttgtagaa
     6301 atggtcatga cagatgaagc ttggtttgtt gttcgaaaca caccaaatgt tacaggattt
     6361 gtcggatcac acgggaacag atcaaaacca actccattat tggaacaaga aattcgtgac
     6421 attttggtat ctatgggaca aactgttcaa gaatttgatt tcgatgttga gattggtcaa
     6481 actgtacgta ttattgatgg tgcttttgca gactacactg gtaagattac agaaattgat
     6541 aataacaaag tgaaaatgat tatctctatg tttggtaatg acacagttgc agaagtaaac
     6601 ctaaaccaaa tcgcagaat
//
LOCUS       DAVKAO010000081         6574 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKAO010000081 DAVKAO010000000
VERSION     DAVKAO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.081"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(169..798)
                     /locus_tag="VWX16_001800"
     CDS             complement(169..798)
                     /locus_tag="VWX16_001800"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9159104.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(800..1582)
                     /gene="thiM"
                     /locus_tag="VWX16_001801"
     CDS             complement(800..1582)
                     /gene="thiM"
                     /locus_tag="VWX16_001801"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9159105.1"
                     /translation="MTSLKLLKEKVPLVICITNDVVKNFTANGLVALSVSPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            1673..1759
                     /locus_tag="VWX16_001802"
                     /pseudo
     CDS             1673..1759
                     /locus_tag="VWX16_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(1864..2532)
                     /locus_tag="VWX16_001803"
     CDS             complement(1864..2532)
                     /locus_tag="VWX16_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9159106.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDFALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(2747..2835)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3089..4225)
                     /gene="lctO"
                     /locus_tag="VWX16_001804"
     CDS             complement(3089..4225)
                     /gene="lctO"
                     /locus_tag="VWX16_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9159107.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(4850..6340)
                     /gene="lysS"
                     /locus_tag="VWX16_001805"
     CDS             complement(4850..6340)
                     /gene="lysS"
                     /locus_tag="VWX16_001805"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9159108.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(6467..>6574)
                     /locus_tag="VWX16_001806"
     CDS             complement(6467..>6574)
                     /locus_tag="VWX16_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9159109.1"
                     /translation="FINTYDLEPMAQQVIPKAAFGYIASGAEDTFTSFQ"
BASE COUNT         1954 a         1408 c         1142 g         2070 t
ORIGIN      
        1 agttcaagaa attgaactgg aaatacagcc ttgcactcac aaagacagca gatctttctt
       61 ttgcaaaaaa caaatgacct gtttgatgaa ttagccattc aagctgaatc tagacatagc
      121 tttttaaaaa aggaaaatcc tacttactta gaatccaagg atagatatct attgttcact
      181 catttcccga acagtttttt ctatattttt tgcatacgat attgccgaaa tgattgaaac
      241 gccgtcaaca ttggtcttta taatgtcttt aatatgtttc gtctgtatcc caccaattgc
      301 aactaaaggc atttgtggca atagttttct catcaattca agaccttcat aacctatagc
      361 accaccagca tcatcttttg actgggtatc aaatacaggc ccaacaccta cataatctac
      421 atattcaact tttgattgtt gaaattcttc ttcgtttctt atagaaagac caattatttt
      481 atctggcatc aattttctaa tttcatcaac accaatatca tcttgaccaa catgtacgcc
      541 atcggcgtca atttccattg ctaaatctat atcgtcatta acgataaatg gaacattgta
      601 ttttttacaa agttctttaa tttggatagc tagctcaagt ttttctaagc cttctaaagc
      661 accctcacct ttttctcgaa attgaaataa ggttatacca ccttttaagg cttcctcaac
      721 gactgtatat agattttttc cttggcaagt agtcgttcca caaataaaat atagttttag
      781 taattcttta tgaaacatct tacttcactc ttttgaattc ctttacatct tcatctgtaa
      841 tctcgtataa ggcatttata aattcaactt taaatgtccc aggaagatgt ccatttggac
      901 gtttttctgc catttctcca gcgatattgt aaactaacat tgctgtttct aatgatttca
      961 attcttgacc tttttctagt ccgataaagc ttgctactac agctcctaat aagcatcctg
     1021 tcccaatgac tttcggcatc atagcactac cattatgaat cgttaccact tctccattaa
     1081 cagcaatggc atccacttca cctgttacta ctattggaat attgaacttc tcatttgctg
     1141 ctagagcaat ttcgtcaata ttatctacgc ccgcactatc tactccttta gatgccacat
     1201 ctattcctac taaagaggca atctcgccag catttcctct aatcgctgct agtttataat
     1261 tgttgattag atcatctgct actttttttc tatattctcc tgctccacag gctacaggat
     1321 ctaaaactgc tgggacatta tatttctccg caattttcag agcagcttgg tataatttcc
     1381 aattttcatc tgtcaatgtt cctatgttta ttaataaacc accagcatac tttaacaaat
     1441 cctctaaatc tgctggaaac tcactcatgg ctggtgagac gctcagtgct actaatccat
     1501 ttgctgtgaa attttttact acatcattgg ttatacaaat gaccaatggt actttttctt
     1561 ttaataattt taaacttgtc atattgaaat ccttccttta cactttatac gatctactga
     1621 tttcgattta tctctatctt tcgttaagaa atttttttca tttacgttga atgatttata
     1681 ctcaatgaaa atcaaagagc aaactaggag gctaaccgca ggttgctcaa aacactgttt
     1741 tgaggttgtg gatagaactg acgaagtcag taaccatacc tacggcaagg tgaaactgac
     1801 gtggtttgaa gagattttcg aagagtctta cctcatcaaa tttgtaaata tcatgagcct
     1861 tctctagaca tcgtaaccaa tatcaaaaaa agctaattct aaagcgactg cttgattcca
     1921 gcgttgctga agttctgtca aatcttctcg atttttaccg acacgattga gttcgtcaac
     1981 cagaaattga acccactctg caaagaaagg acctctgtgg agattgatcc attccgaatg
     2041 aatataaact tcaggtagag caaaatcttt agaaccccag tctaaataga gaccttctgc
     2101 aatgaccagc ataaccaaaa gatgggcata gtctgatgaa gccactgccg aatacattag
     2161 atcctgaaag gcttttgtta cagggtgcaa agtcacttct aggtagtcat tctctgctac
     2221 ttttaactct ttaaaagcct tttggaaata accatcttca tctgcttcaa gaaagcctag
     2281 ttgcttggca aaacgaagct tggattcaag tttatctgcg tgagctacgc aggcacccag
     2341 catggataag aaggcatcaa agaagtgata atcttgaatc agatagtcct ttaagacctt
     2401 attctcaatt gtccccgcaa aaagttcctt aacaaaacga tgattgattg cagcctgcca
     2461 atccttctga ctgcttttta ataattctcc aacagtcaaa cctggctgaa atgcatagtc
     2521 ttgtgtttcc atatttactt ctcctctctt tacttgttag taattaataa aacaccaaga
     2581 aatatcaagc aaaatcgtaa ttccacttga tccttttaaa acacatcgag agtatttgca
     2641 gagagctaac taaacaagcc tatccagttt atataaacaa aaaactccaa ttacaatcaa
     2701 gaattagagt tgacttacaa gattagaccg ttcatttcac catacgaaaa aactgttcac
     2761 atttcccttc gccagtctta actgtatcag gttcaatggg tattatctca gcctaaagca
     2821 ccccaaatgt ctctattatt taattactga accagtatag caaaaaatga aagccttagc
     2881 aagatattta accgaaaaat atctttatat atagtagatt gaaactagaa tagtacccct
     2941 ctacttctaa aacattgtta gaaatcgatt taactgtcct gattgatttg tcctattctt
     3001 atttcatttt actatagttt tcgatagcaa tttattcttc caatacacga agaaaaacct
     3061 ccacattcag tggaggcaat ctgttttatc aatacaattt taagtcacga gggtcaactg
     3121 ggaaggttgg gttgtatgga ttgtgacgga gcttaaagtg tttgacatct tcaatggtct
     3181 gagctccaga caattgcata actgtcttca attccgcatt caagtgttca aagacttgac
     3241 gcacaccgac actaccaccg agagccaagc cataaataac agggcgacca atagctacca
     3301 agtctgctcc tgaagccaag gctttaaaga cgtgttgacc acgacgaaca ccagagtcaa
     3361 agacaatcgg cacacgtcta tcaactgctt ctgccacttc ttgaagcgag tcaaaggcag
     3421 ctggtccacc gtcgatttga cgaccaccgt ggttggttac ccagatacca gaagctcctg
     3481 cagcaagcga acgttcaacg tcctcacggc attgtggtcc cttgacatac acaggaagac
     3541 cagagtattc agcgataaat tctacatcgc gtggagacaa gcgttgttta gctgatttgt
     3601 aaacaaagtc cattgattta ccagcacctt ctggcaggta ttcttcaaca atcggcatgc
     3661 caactgggaa gacaaaacca ttacgcttat ccacttcacg attcccccct acagtagcat
     3721 ctgccgtcaa gacaatcgct ttataacctt cagccttcac acggtccatg atgtggcggt
     3781 tgataccgtc atccttacta aagtaaaatt ggaaccaatg gggggtccct tgaagggctt
     3841 cagaaatctc tggaaggtca acagtagagt aagagctggt tgtataaaga gaaccaaact
     3901 catgcacacc acgcgcagtc gccacttccc cctgttcatt tgccaattta tgagccgcaa
     3961 caggcgccat aatgattggt gaagatagtt tttcacctgc aaattcaatc tctgtacttg
     4021 gattttcaac atcgcaaagt gtatgaggaa cgatgagttt gtggttaaag gcgcggatat
     4081 tctctcttaa agtgaaagta tcttccgccc cactagcgat atagccaaat gctgctttag
     4141 gaataacttg ttgcgccatt ggctccaaat cataggtatt gatgaagtct acatgacctt
     4201 ctgcattgct tgttttgtat gacataaaat gccctccttg attaagtaag cgtttgcttt
     4261 ttactacaca actataataa ctcttttttc aatcattttc aagagttttg cttaaaaacc
     4321 atcttatttt ttcatacaat atactagccc tactttcctt aaactctaaa ccttagtcta
     4381 tattaagtga atcgttgaga ttttttgctg ccaggcattt gaactacttt tttatttgcg
     4441 gattctatct actaatacta taaaaaaaca agtgaataag acaccagaac atcctctaat
     4501 aggacctatg gacttatatc agtcccatat atactgttta agcaagatac tatcctagtt
     4561 gacgctaact tcctatccca cttattaatt ttttatttat agtgaatctg aacaaatcaa
     4621 ttggggaatc caagtcaatt tctagtactt tttgaacagt ttcagcgtac tatcgtaaaa
     4681 tgaaataaaa catgcacaaa ttgattaggg aatttaaacc aatttctaac aatgtcttag
     4741 aaatcaaagt gtactatttc aacttcaata tactacactt cattttctcc ttaaattcag
     4801 tctctttttg aatcaaaaaa tcctctggta agagccagag ggtaagaatt tatttcattg
     4861 ttgggaagag caatacatca cggatagttg ttgtatcagt gaggagcatg cagagacggt
     4921 cgataccgat tcccaaacca cctgttggtg gcataccgta ttcaagagcc tcgatgtagt
     4981 catagtcgat gcctgtcgct tcatcatcac caagttcttt agctttagct tgggcttcaa
     5041 aacggctaag ttggtcgatt gggtcgttca actcagtaaa ggcattaccg tactccttag
     5101 tcatgataaa gagctcgaaa cggtcagtaa agcgttggtc ttcaggattt ttcttagcga
     5161 gtggagatac agctactgga tgtccataga caaaggttgg ttggattaaa gtttcttcaa
     5221 caaactcttc aaagaaggca ttgatgatgt gaccaacctc agtgtagtgt ttctcaactg
     5281 gaactttctt ctcagcagcg atagctttag cttcttccaa agtcatgtct tgccagaaat
     5341 cgacaccagt aatttctctg atagcatcca ccatatgaac acgcttaaat ggttcgttaa
     5401 tcttgatttc agtaccttgg tagttgactg ggccatcacc tttgactgat ttagcagcgt
     5461 gttggataat gccttcagtc aagtccatga tgtcttggaa gtctgcataa gcttggtaaa
     5521 cttcgataga agtgaactca gggttatgag tagcgtccat tccttcgtta cggaagatac
     5581 ggccaatttc atagacacgt tccataccac ccacgataag gcgttttaag tgaagctcag
     5641 tcgcgatacg aagcaccatg tcaatgtttt gggcattgtg gtgggtgata aatggacggg
     5701 cagcagcacc accggcttca ttatgaagaa caggtgtttc cacttcaagg aatccttttt
     5761 ggtcaaggta acgacggatt tcagagatga tttttgaacg agtgacaaag cgttcaaagc
     5821 tttcacgatt agaaatcaag tcaaggtaac gtttacggta aattgtttca acgtctgtca
     5881 aaccatggaa tttctcagga agaggacgaa gagccttaga caagtgtgtg atgtgggttg
     5941 ccttgataga gagttctccc atatccgtac gcatcacttc accttcgaca ccaaggaagt
     6001 caccaaggtc tgcttttttg aagatttcgt agttttcttc accgacagca tccttacgaa
     6061 cgtagatctg aatctggcct tcgcggtctt gaaggtgggc aaaaccaact tttcctttac
     6121 cacgtttggt taccaagcgt cctgcgatag tagctgtttc gtttttatcg tgtaattgtt
     6181 ctttatcgag gttggcatat ttatctttta attcttgtga atttgcagta cgttcaaaac
     6241 gttttccgaa aggatcgatt ccttgttcgc ggagcgcagc cattttttca cggcgaacga
     6301 tctgctggtc atttagttct tccatatgtt ctgtagacat gggatcctcc tagttttact
     6361 caaaattgaa tacgatgagt cattttttgc gataccccat tttatcataa ataagcaaga
     6421 aattcacgga taatctttaa aaactaaaaa gaacctgacg ctaaaatcac tggaaagaag
     6481 tgaaagtatc ttccgcccca ctagcgatat agccaaatgc tgctttagga ataacttgtt
     6541 gcgccattgg ctccaaatca taggtattga tgaa
//
LOCUS       DAVKAO010000082         6203 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKAO010000082 DAVKAO010000000
VERSION     DAVKAO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.082"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..463)
                     /locus_tag="VWX16_001807"
     CDS             complement(<1..463)
                     /locus_tag="VWX16_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159110.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLNEDYYNILVHEKETIQGYLVLSNCG"
     gene            complement(453..650)
                     /locus_tag="VWX16_001808"
     CDS             complement(453..650)
                     /locus_tag="VWX16_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159111.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(654..1238)
                     /locus_tag="VWX16_001809"
     CDS             complement(654..1238)
                     /locus_tag="VWX16_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159112.1"
                     /translation="MNVKKIMLIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQYVLK"
     gene            complement(1647..2072)
                     /locus_tag="VWX16_001810"
     CDS             complement(1647..2072)
                     /locus_tag="VWX16_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159113.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEVASISLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2201..2584)
                     /locus_tag="VWX16_001811"
     CDS             complement(2201..2584)
                     /locus_tag="VWX16_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159114.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKETVLG"
     gene            complement(2606..2812)
                     /locus_tag="VWX16_001812"
     CDS             complement(2606..2812)
                     /locus_tag="VWX16_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159115.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(2799..2960)
                     /locus_tag="VWX16_001813"
     CDS             complement(2799..2960)
                     /locus_tag="VWX16_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9159116.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3319..4275)
                     /gene="lytA"
                     /locus_tag="VWX16_001814"
     CDS             complement(3319..4275)
                     /gene="lytA"
                     /locus_tag="VWX16_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9159117.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(4651..6021)
                     /locus_tag="VWX16_001815"
     CDS             complement(4651..6021)
                     /locus_tag="VWX16_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9159118.1"
                     /translation="MNKKRTVDLIHGSILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPRFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         1981 a         1304 c          965 g         1953 t
ORIGIN      
        1 gaccacaatt actcaatacc aaatacccct gaatggtttc tttttcatgc accaatatat
       61 tataataatc ttcattcaat aataaggctg ataaactagc atcttggtca aaatgtaagg
      121 gaatttctcg accgtccttc tttaatggat attctggtaa gatactaaat agttcaatca
      181 tagaaggaaa ctcaggatta gttgttgtaa atcgaaaaag ctgcgctttc tcatctttct
      241 gacttccttg atactctccc aattctaaaa aatgattcaa ggtagtataa aattccttat
      301 tttttacttc atcaatgatg accatatcat aatcttttgt tgtgcgactt ttaaatcctt
      361 gcgaatccaa tacgatagag gtagcagttc ccccaatcag aacataatag ttctgaaaat
      421 ccgcaaacat ttcttgaaaa ataactttcg tattagctgg catcatcttc tcccagatac
      481 tgtaatatca tattttctag tgcttcactc tcttcctcta tacgtgggtc atcatcatct
      541 tttaaggtca aataaagaga aatcggatct acaaattgtt tatcatgatt atttttaaaa
      601 tcattccaaa actcagatac aaaaggacga tatttccata tctctagcat ctttcatttt
      661 aaaacatact gagaaagtgg caaggataac tgattgaatt ttctctgcca tatgacatag
      721 ctagtatttt catccgtttc agctaaaaaa gttgaatgcg acaaagcata agcaccacca
      781 tatagaaggt tagaaacaga ttttatctgc tttatatcgc catctggcaa taaaaaggca
      841 atccacgtta attgttcgct aggtgttaat tccttaggga tttcagtatc attcgcattg
      901 agtactagtc ccaatggagg gaagaagagg tttcccttaa agtctacaaa cggaactcta
      961 gcttgaagta attgcttttt ttcactgtct gataacttcg aaaacaccaa aacaacatcc
     1021 atattggctt tttcacccat agtgcgagcc tgagtaacaa atgaactcaa actccccctt
     1081 ctcttttctt taatcaataa aaatgattcc ttatgaaaag tcatttgagt atactcaaac
     1141 ctttttacaa aactgattgg taaagtcaaa gttagttcct caatactgac atcaacataa
     1201 aaggattgaa aaattaacat tatctttttg acattcatgt tttttcctta ttctatcatt
     1261 tttattatat tttatcattc ggtataataa aatataagtt ttttgataaa ataaccaaaa
     1321 actactcgct atcccctctc acattaccct acatatcgtt tgacatgcga ctgatagttc
     1381 aggaaaactt caaggagaac ttttctctca tccactatgc aggacttact atttcacttc
     1441 tacatcctat aggctcaatt tgacactttt ctttttcgaa ttgctcatat gcctcctgaa
     1501 tagtgagttc gatttatcca aatttatttt tgtctatttt gatgaatact tccacgaaga
     1561 tagtctgaaa atgaatttgc accaattttc cttctctttt taagcatatt attccagata
     1621 tgattgccct cttgttcagt aataaattac gctttaaacg cttcaatcag tatatctcct
     1681 gttgtcatat gttctaaaga aaattcttct acatatggtt taacatctct taggttatta
     1741 cttcctaata tcccattatg ctttttcgct aaggaaatag aagccacttc tcccttacca
     1801 ataatcttgt tactatcatg atttcttgtt aaatctctat ataatgcgta ttcttcagtt
     1861 ccaatgtcta tgctcacaat ctcagctgaa cccttagtta ccaactggtc tatcctagat
     1921 tttaaatggg gaattgtagg tatattgatt tcatcataca cctcttgtgg aataacaatt
     1981 ttacccgaat agagcttttc taaaagatgt tcagtgccaa cccataaaaa tactgaaata
     2041 caatctgcat caataaatac acgactagtc aaacgacaac tcccccctct tcatatagcc
     2101 catatacaat atcatatctg aaagcatcta gtaacagttc ctcatacttc ccttgcgaaa
     2161 ttctgttatt ttctaaaagt tgttcagttg aattaatata ttatcctaac accgtttcct
     2221 ttttactttc tgacaaagga cgatataaac ttgtatcata gcctaatctt gaagctgtct
     2281 ctataacact aatatccata tttttaattt cttctgcatc aaggtatcca tcattcctca
     2341 atctatataa catagcttta tgactgatac cataaaactg acccaatttt ataatatctt
     2401 ctacttcaag atgagttcta ttggcatttt ctctaatttc ctcaaccatc ctatacagtg
     2461 aagatgggaa aattaaaaaa taagaagcaa actgatccgc ttttctttca gtttcatctc
     2521 cttcaccaat caagataaga ctgactgaac tcttcttcac ctcatcataa taaagatgat
     2581 acagttcatg tgctaaagaa aatctttacc ttcctaatga catgtctgaa ttgactgcaa
     2641 tgagactgaa ctgagttcct ttataacaga ctccgctaat attctttccg agtccataaa
     2701 ataccagcgt caaattttct atcttttgta ccaatttaaa aatatctatc ggcgattcac
     2761 catcagctcc caactttttt ctaagatttg gagctttatt acttaaatcc attcgtgata
     2821 ttcctttcac ctttttccat tttggcaatc atgctttgat tcaaagacaa atactcagca
     2881 atcttatctt gagtaagatt tgaagatatt ctaagttctt ttattctttt tccaacatca
     2941 catacattta tcacatacat ttatcatatt catacacctc tcataaaaag aatagcacac
     3001 tcttgtcata attttttaaa taaaaaaatt atgacaaaac aaggaagcaa tttattgatg
     3061 ctgcttaaaa atctaaaatt gatgagttcc tatcaaaaat cgtatcttca acctcaaaac
     3121 agtacttaaa gctatccgac tcggtttaca ttgtcaaatt tagattttat ttgagcataa
     3181 ctttctagtt tgctttctga tttttgttta atatagtagc aaacattgaa ggaaatatct
     3241 ccacaaaaaa acgcatacta ttaagctttt tcaagaccta ataatatgcg ctgttctgat
     3301 ttgaaagaca ttccattatt attttactgt aatcaagcca tctggctcta ctgtgaattc
     3361 tggcttgtct gccagtgttc cgtctggttt gaggtagtac cagcctgttc cgtccgctga
     3421 ctggataaaa gcatttgata ccatggcgcc ttctttagcg tctaagtagt accaagtgtc
     3481 cttgtacttg acccagcctg tcttcatggc accttcttcg ttgaaatagt accacttatc
     3541 agcgattttc ttccagcctg tagccatttc gcctgagttg tcgaaccagt accagttgcc
     3601 gtctgtgtgc ttcctccagc ggtctgcaag catatagcct gaactgtcaa agtagtacca
     3661 agtgccattg attttctcaa acttgtcttt tggataagag ccgtctgaat gtacgtacca
     3721 gtagccagtg tcattcttct gccagcctgt ttcaatcgtc aagccgttct caatatcata
     3781 cttaaactgc tcacggctaa tgccccattt tgccaagtaa gggtatggat ccacatggtc
     3841 tgagtggttg tttggttggt tattcgtgca atactcgtgc gttttaattc cagctaaact
     3901 ccctgtatca agcgttttcg gcaaacctgc ttcatctgct agattgcgta agagttcgat
     3961 ataaaggcgg tagtccgtca tgaactcttc tttagttgaa tggctttcaa tcagttcaac
     4021 cgctgcatag gtctcagcat tccaaccgcc cccaacgtcc caggcaccat tatcaacagg
     4081 tcctacctgc atgatgcaac cgttcccaac aatgtgcgag aaaaaaccta attctgggtc
     4141 tttccgccag tgataatccg cttcattctg tacggttgaa tgcggattcc cagttgagtg
     4201 tgcgtgtact tgcctatatg gttgcacgcc gacttgaggc aaatctgttc ttaatttact
     4261 cacattaatt tccatattct actccttatc aattaaaaca actcattttt tacaatccag
     4321 aaccagaaac tcctttattt ctaccttaca aagaagacaa tcttagtcac gattaggctt
     4381 gtagatagaa cctcaaaacg cactattttg acactgtaaa taggactgac aaggtctgca
     4441 ttctatctac aataacaccc cagactaaaa agcttttcaa aagtatattt ttacagtctc
     4501 tatatgtcct tttcataaat actatacttt attatatcat ataaaagaag tcaaaagtct
     4561 gttaaactat tttcaacacc aaactaaaga agagaacaca agttttttcg atgttcacta
     4621 gaggaaatgg attttattca gtaaatccaa ctaggattgc actttggttg ccaaaattgc
     4681 ctttccttct tttatcaagg gatgacggaa tagtgagaag tacagttgaa ctgtcatggc
     4741 aacccagata agaggttcac aaaggataac acccttatat cctgcccaag gaataatcaa
     4801 aaccacaaaa acgatttttc cgattagttc aataaagcta gaaactagag gaaggatctt
     4861 ctgccccaag ccctgcaagc aattgcgata aatcaacaag aggctcaaaa tgggataaaa
     4921 ggctgaactg atttgcagat agagacttcc attttctatc aagtaaccat ctgtcgaact
     4981 agccaagaag gaaaccaagg ctggactggc aaaaaagagg aaaatacaaa caaaaactgc
     5041 ccaggatata cttaaacgac tgccgattcg aagaccttga acaatgcggt caggtcgctt
     5101 agctcctaga ttctgagaag caaaggtcgt cattgatgca gaaatagcgg tcataggaag
     5161 aagggcaaag gtcataatgc gtcgagctgc cgtctgggca ctaataatca ctgcaccaaa
     5221 tgtattaaca gaaaactgta aaatcacact gccgatagat acaattgaac tcatcaagcc
     5281 catagccaaa ccttgctcca agagatccgc gtacaagctt ttgtcccatt tgaaatgttt
     5341 aaaccgtggc aagagttctg gcacactttt acgaatataa taaaagcaga gaaccgctga
     5401 taaaccttgc gaaataatgg tagcaagtcc tgcggattga actcccagat gcaattgcgt
     5461 aataaaatag agatccagaa ccacattaac caaggcagag aaaatcagaa atcccagggc
     5521 tgctagactg tcaccaatag accgcaacaa gcctgcaaaa agattataag caaagctgac
     5581 acctacacag gtcacaatca tagaaatata ttgataagat tgaggaagaa tttctgcagg
     5641 agtatctaag tattgcaaga gaggatacaa gccaagaaag cccagcaaca taactagaat
     5701 gctcaaaaga gcacctaaaa tccaggtggc tgctactgct tccttgattt tagtgaaatt
     5761 ccgagcccca taataacgag caatgacaat ccccatgcca ttgccaacac caagtgtaaa
     5821 acctacaatc aggtcaaaaa tcgctgtcgt cgctcctact gcagccaagg attcttgacc
     5881 aagaaatcgt ccaacaatca agacatcagc agtgttatag agctgttgaa aaatatttga
     5941 tagcaagatt ggaaaggtga agcttaagag cgagggaaga atcgaaccat gtatcaggtc
     6001 cactgttcgt tttttattca taaagctatt atatcagctt tccagaggag cgacaagaaa
     6061 cagcaaactt atatatagcg gaatgaaata aggacagtca aatcgatttt taacaatgtt
     6121 ttagaagtag atgtgtacta ttctagtttc gatctattat agttggttga gtttggaata
     6181 gtacgctgta gctgctaaaa cat
//
LOCUS       DAVKAO010000083         6199 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKAO010000083 DAVKAO010000000
VERSION     DAVKAO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.083"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(112..387)
                     /locus_tag="VWX16_001816"
     CDS             complement(112..387)
                     /locus_tag="VWX16_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9159119.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(494..1333)
                     /locus_tag="VWX16_001817"
     CDS             complement(494..1333)
                     /locus_tag="VWX16_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9159120.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1475..2323
                     /locus_tag="VWX16_001818"
     CDS             1475..2323
                     /locus_tag="VWX16_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9159121.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILGWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2416..3333)
                     /locus_tag="VWX16_001819"
     CDS             complement(2416..3333)
                     /locus_tag="VWX16_001819"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9159122.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3771..3976
                     /locus_tag="VWX16_001820"
                     /pseudo
     CDS             3771..3976
                     /locus_tag="VWX16_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4287..4580)
                     /gene="rpmA"
                     /locus_tag="VWX16_001821"
     CDS             complement(4287..4580)
                     /gene="rpmA"
                     /locus_tag="VWX16_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9159123.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4597..4941)
                     /locus_tag="VWX16_001822"
     CDS             complement(4597..4941)
                     /locus_tag="VWX16_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9159124.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4957..5271)
                     /gene="rplU"
                     /locus_tag="VWX16_001823"
     CDS             complement(4957..5271)
                     /gene="rplU"
                     /locus_tag="VWX16_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9159125.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5308..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5490..6089)
                     /locus_tag="VWX16_001824"
     CDS             complement(5490..6089)
                     /locus_tag="VWX16_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9159126.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDIR
                     DKLLTLIAFMMGVFLLTIYKEKLRIVKKSILSLIPLAILSIIIGFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVVFSVYLTIFYHEKTILGVPIMMSIFYLSMLFA"
BASE COUNT         1903 a         1266 c         1033 g         1997 t
ORIGIN      
        1 aatattttcg gaacattcaa ctttttaact ctatttatta ctaaatttca taattaaaaa
       61 gcctactgac caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac
      121 agcgtcttta agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat
      181 ttctttacca gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt
      241 accaaaaccg atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa
      301 tacagcttca actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt
      361 agcgatcaaa tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac
      421 aaatataatc atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat
      481 tttatttatt tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt
      541 gagtttgaat aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta
      601 agctttcttt catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg
      661 ctacagcacg ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag
      721 ttccacgacc tttaaccatt ggctgcaatt catggtcttt catttgcatg acaacacgga
      781 tattgagaag ggagctcaac aagccagtta cacggccaat tcgtccacct ttgacaagat
      841 tttccaaagt tgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag
      901 ataaaattgc ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga
      961 atttcagggc ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc
     1021 tagcaccttg gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa
     1081 gaatctggcc accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg
     1141 gctgacttgt tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt
     1201 ctttcaaatc cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg
     1261 taatatctaa ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct
     1321 taatttttgt catagtatca atctttctat tttaggattc agattggttt ccttacttct
     1381 aattatatca aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt
     1441 tgatataatc aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt
     1501 taccatttta ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca
     1561 tcatctcttt gaaggagggg cgacaggcat taccctcatc accttttatc tttttaaaat
     1621 ccctgtttcc ctcatgaacc tgctgattaa tattcccctt ttcatcctag gttggaagat
     1681 ctttggagcc aaatccctct attctagttt actaggaacc ttagctttgt ccggctggtt
     1741 agcttttttt gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc
     1801 ccttatagcg ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac
     1861 aactggcgga actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg
     1921 aaaactgctc tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga
     1981 tttgagattg gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt
     2041 gattggtgaa ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca
     2101 acttgctaag gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaagg
     2161 ctactatagt aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt
     2221 gaaaacgaag gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc
     2281 ccatgaaatc ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg
     2341 acctcaaaag ttagactaaa tcatctatct tttgggttac agacaacctc ttttttgttt
     2401 tatttactca agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc
     2461 cactaattgg tcaactgagt caaatttggt catatctcga atgcgatcaa gccaataaac
     2521 catgacggtt tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc
     2581 ttcttctcca tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct
     2641 ttgaatctca acatcaacga cataaacgcc atctgctggc atataagtac ggtctaaaag
     2701 cactaaattc gctgtcggat aaccaattgt acgaccacga gcattaccat gaactaccat
     2761 acctcttgat ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa
     2821 aatagcttga cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac
     2881 aatgataact tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc
     2941 aaatgtataa tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc
     3001 aaagaattct tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc
     3061 ttctacacct tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa
     3121 caaatctgga tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac
     3181 gataggcaat aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc
     3241 cttatgtatg ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc
     3301 tttttggttt tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta
     3361 tttctggaac agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tgttttcaaa
     3421 aggcacttta ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg
     3481 taggacatct tcaaaatttt gcaaggagtg attagacttg ttcggtaacc ataaagtgtc
     3541 atactatgct tatgtatgaa aaagcaatgc aactaactcc tgagaacttt aaattactaa
     3601 ttggtgccga aaaggtagaa tttagaatcg aggtacacct atggctgtaa aatttacaaa
     3661 atgagacaac ttgggcaaga tgtttgaaga atttcctaaa ctccctgatt tgaagcaagt
     3721 cactttccct gatgacaaag aaaaaagcca aagcaataaa gaaaaactag atgactgctt
     3781 tccaacaact cccatctagt gtgcttcaga ctgggctatt tttctttcca tctgttagct
     3841 tggattctca gaccgtttca gctaaagaat atcttttcct ttatcagaag gaacggctca
     3901 agccattcag acaagtgaag ggacgacaag ccaatatttg aaaccaggta gcagttctta
     3961 tagtcaattg aaataaaatc tgaagaaatc gagtaggaaa cttaaatcaa tttttagcag
     4021 tgttctattc cagattcata ctcaatgaaa attaaagtgc aaactaggaa gttagccgca
     4081 ggctgtactt gagtacggca aggtgaaact gacgtggttt gaatttgatt ttcgaagagt
     4141 atcaatccac tatacatatt aaaaagtatg ggtaccaaaa caaaaaaaca ggctctccga
     4201 aaactcggaa agccttattt taagctgttt ctagcttcct cacctttaca tttcaaggtt
     4261 cgggataaaa ggttcaatgg acctttttat ttagcgattg ggtaaacaga aacttgtttt
     4321 ttatcgcgtc ctttacgttc aaagcgtact acgccttcaa ctttagcgaa caaagtatcg
     4381 tctccaccac gaccaacgtt tacacctgga tagatgtgtg taccacgttg acggtaaagg
     4441 attgatccac ctgttacagt ttgtccgtca gctgctttag ctccaagacg tttcgcttgt
     4501 gaatcacgtc cgtttgatgt agaacctcca ccttttttgt gggcgaaaag ttgcaagttg
     4561 ttaagagtca tttttaacat aatgttttcc tccgtgttag ttttctgtga taactctggt
     4621 ttggacgaac tcagaagagt tctccgataa gtttgccata cctaagaaaa atgattcaaa
     4681 gaataactgg gtcatttctc tctggtgtga aggaagatct tttggtattt caaccatcag
     4741 atagccacct tcatcttcgt ttaattctag gattggttca tagcctgcaa atttctcaat
     4801 agaattgata aagttaatgg caagcgtaga aaccgatgca cacacgacat ctaagccgta
     4861 ttcgccactc tcggcgtgtc cagtaatttc cgcactcctc agctcgccat cttcggctct
     4921 ctcaaagact gcttgtatca tgtgttctcc ttaaaattaa gcgttaattg cgttgatgac
     4981 aacttttgta tatggttgac ggtgaccttg tttacggtgg ctaccttttt taggtttgta
     5041 cttgtaagta acaactttct tttgttttcc ttgtttttca acagttccaa ctacagtagc
     5101 tccagcaaca agtggagttc cgacaacagt gttttcacca ccaacaagaa caacttcgtt
     5161 aaaagtaact tcttgaccag cttcaacgtt caatttttca atgtaaactg cttgaccaac
     5221 ttcaacttta acttgttttc cgccagtttt gataattgcg tatgtgctca ttatgcacct
     5281 cctatgattt ttatgggttt ccccgatttt tttgtgaaga ctcgcctagc atcgtgggac
     5341 aaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5401 tagtcaactc ttcaagtgta gcatatttcc tattttctta caagtaataa cacctaaaat
     5461 caagcttttt cttttacttt tttctgccac taggcaaaaa gcatactgag ataaaaaatg
     5521 ctcatcataa taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaatagact
     5581 gaaaagacaa cgccaaggac aaaactacta agcaggctaa caaatatgaa cccttcacgc
     5641 aaaaaaggag tgtgcttagt tcggaaataa tctccaaaag ctagcattgt tcgtttgata
     5701 ttccctgtca taaaggcatt attgtaggca atacccgaaa cctcaccaaa agcagttgtc
     5761 accagtccca tacagaaggc caagggcggc actagataga tattatctac agtttgtggc
     5821 acaaaaccaa taattattga taggattgct aaaggaatca aggacagaat agattttttc
     5881 acaattctca atttttcctt ataaatcgtt aataaaaaga ctcccatcat aaacgctatt
     5941 aaggttaaaa gcttatccct aatatccgaa acattatttt gtatgagctc taccgaaaga
     6001 aagacaacat ttccagcttg tccagctaca agggtattcc cgcgaacaat aaaagtgtaa
     6061 gcatccacat atccagcaca aaacgtcaaa aaagtgctaa ccttttagac tgacgtgata
     6121 tttttcttat aggtaataac ctcattttcc ctcccattgt attttctctt agaaatattg
     6181 taccattttc tttctaaaa
//
LOCUS       DAVKAO010000084         6146 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKAO010000084 DAVKAO010000000
VERSION     DAVKAO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.084"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..358
                     /locus_tag="VWX16_001825"
     CDS             <1..358
                     /locus_tag="VWX16_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9159127.1"
                     /translation="YREALITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVV
                     VVRLEKSVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSK
                     ISEPEKLYAYIAEAIA"
     gene            396..698
                     /locus_tag="VWX16_001826"
     CDS             396..698
                     /locus_tag="VWX16_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9159128.1"
                     /translation="MIKILAACGAGVNSSYQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIRLPAKQNMV"
     gene            706..1510
                     /locus_tag="VWX16_001827"
     CDS             join(706..1074,1076..1510)
                     /locus_tag="VWX16_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9159129.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANDSGKKKFHAMKAQEIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLIAEDKAYNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHKLEF"
     gene            1559..2023
                     /locus_tag="VWX16_001828"
     CDS             1559..2023
                     /locus_tag="VWX16_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9159130.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2180..2605
                     /gene="lacA"
                     /locus_tag="VWX16_001829"
     CDS             2180..2605
                     /gene="lacA"
                     /locus_tag="VWX16_001829"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9159131.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2636..3151
                     /gene="lacB"
                     /locus_tag="VWX16_001830"
     CDS             2636..3151
                     /gene="lacB"
                     /locus_tag="VWX16_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9159132.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHVETHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3162..4091
                     /locus_tag="VWX16_001831"
     CDS             3162..4091
                     /locus_tag="VWX16_001831"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9159133.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4093..5073
                     /gene="lacD"
                     /locus_tag="VWX16_001832"
     CDS             4093..5073
                     /gene="lacD"
                     /locus_tag="VWX16_001832"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9159134.1"
                     /translation="MALTEQKRARLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5307..6143
                     /locus_tag="VWX16_001833"
     CDS             5307..6143
                     /locus_tag="VWX16_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9159135.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
BASE COUNT         2033 a         1064 c         1276 g         1773 t
ORIGIN      
        1 ttatcgtgaa gccttgatca cgcgtgaaaa gtcatttcca actggtttag atatggaatt
       61 tctaggaaag gacttgccaa atgtagcgat tcctcacaca gatattgttc ataatctagc
      121 tgaaaaagtg gtggttgttc gattagaaaa atcagtaact tttcacaata tgatagctcc
      181 tgataaggaa gtagaagtat ccctactctt ctttatcatt aacaattcaa gttcaagtca
      241 aacaaatatt ctggctcagt tgatggactt tttcacagga aatggacatc ttgaagattt
      301 atcaaaaatt tccgaaccag aaaaacttta tgcttacatt gctgaagcaa tcgcttaatc
      361 ttgtctatta aaaaataaaa tcggaggaaa tccaaatgat taaaattctt gctgcctgcg
      421 gtgcaggtgt taactcaagt taccaaatta aaagtgctct agaagaagaa ctttcaaacc
      481 gtggttatga tgttcactgt gatgcagtca tggtgaaaga tgtaaacgaa gaccttatga
      541 aaggttacga tatctttaca ccaatcgctg caacagatct tggttttgaa ccaggtatcc
      601 cagttatcga agctgggcca atcttattcc gtataccagc aatgagcgct ccagtatttg
      661 acaatattag actccctgcg aaacaaaata tggtatagta gttctatgaa tgatgaagca
      721 agtaaacaac taactgatgc acgatttaag cgtcttgttg gtgttcagcg taccactttt
      781 gaagagatgt tagctgtatt aaaaacagct tatcaactta aacacgcaaa aggtggacga
      841 aaacctaaat taagcctaga agaccttctt atggccactc ttcaatatgt gcgagaatat
      901 cgaacttatg aagaaattgc ggctgatttt ggtatccacg aaagcaactt actccgtcgg
      961 agccaatggg ttgaagtaac tcttgttcaa agtggtgtta cgatttcaag aactcctctc
     1021 agttctgagg acacggtaat gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa
     1081 agaattagcg aatgattctg gtaaaaagaa atttcacgct atgaaggctc aagagattgt
     1141 cacaagtcaa gggagaattg tttctttgga tatcactgtg aactattgtc atgatatgaa
     1201 gttgttcaaa atgagtcgca gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg
     1261 ttatcaaggg ctcatgaaga tatatcctca agcacaaact ccacgtaaat ccagcaaact
     1321 caagccgcta atagctgaag ataaagccta taaccatgcg ctatctaagg agagaagcaa
     1381 agttgagaac atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa
     1441 tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct ggtattatca atcataaact
     1501 agaattctag ttttgcagga agtctaataa agaaactagc aaggtcaggc cttactttat
     1561 tggtggacat ccaggattta attgtcttct acttgatgat gaagtctatg aagattatta
     1621 tctagagttt gagaaagaag agatttgctc tgttccacgt cctttcccag aaacaggtat
     1681 gttggatttc caagatagaa gtccatggct agagggacaa aaagaaatag atcttagtta
     1741 tgatcttttc agcacagatg cagtgacttt ggatgaattg caatctcgaa caattgccct
     1801 tcgttctctt aaacatgata agggattgaa agtgcatttt gcagaatttc caaacctcat
     1861 catctggtca actttgaaca agggaccttt cattaccttt gaaccatggt ctggcttgtc
     1921 aacattcctt gaagaaggag atcatttaga agataagaag aatgtttgtc tcttagaagc
     1981 caatcaggtt gaagaattag ggtttgagat agaagtttta taaaaaagta aaaaataaat
     2041 aacaaaaaca aacacaaact gttgacttct tcaaacaata gtagtatatt atgtttgtga
     2101 ggactaattg actgtttttt aaagacagcc aatacactgc tggaatccag catagaaaaa
     2161 ataaaaagga gtatacaata tgtctattgt tatcggtgca gatgctgcag gtttgagatt
     2221 gaaagaagtt gtgaaagact tcttagaaaa agaaaacttc caccttgtgg atgttacagc
     2281 tgaaggtcaa gactttgttg atgtgactct cgctgttgct gcagaagtaa acaaagaaga
     2341 acaaaacctt ggtatcgtga ttgatgctta tggagctggt ccatttatgg ttgcaactaa
     2401 gatcaaagga atggttgctg cagaagtatc tgacgaacgt tcagcttata tgactcgtgg
     2461 ccacaacaac tcacgtatga tcactatggg ggcacaactt gttggtgatg aattggctaa
     2521 aaacatcgct aaaggatttg ttaatggtaa atacgacggt ggacgtcatc aaatccgggt
     2581 tgacatgttg aacaaaatgg gctaattaga ttacagtaag aaaggtaagt taaaaatgag
     2641 aattgcaatt ggatgcgacc acatcgtaac agatgaaaaa atggcggttt cagaattttt
     2701 gaaatcaaaa ggatatgaag tcattgactt tggtacatat gaccatacac ggactcacta
     2761 cccaatcttt ggtaaaaaag taggggaagc tgtaactagc ggccaagctg atcttggagt
     2821 atgtatctgt ggtactggtg ttggtatcaa caacgctgta aataaagttc caggtgttcg
     2881 ctctgccttg gttcgtgata tgacaacagc cctttatgct aaagaacaat tgaacgccaa
     2941 cgttatcggt tttggtggta agattactgg tgaattgctc atgtgcgata tcatcgaagc
     3001 tttcatccat gctgaataca aaccatctga agaaaacaaa aaattgattg cgaaaattga
     3061 acatgttgaa acacacaatg ctcaacaaac agatgcaaac ttcttcactg aattccttga
     3121 aaaatgggat cgcggagaat accacgacta agaggtgacc tatgatttta acagtcacaa
     3181 tgaacccatc cattgatatt tcctatccct tggatgagtt gaagattgat actgtcaatc
     3241 gtgtggtaga tgtaaccaaa acggctggtg gtaaagggct caatgttaca cgcgtacttt
     3301 cagaatttgg cgattctgtt cttgctactg gtttagtagg tggcaaactt ggtgagtttt
     3361 tggttgaaca tatcgataat caagtaaaga aagatttctt ctcaattcag ggagaaactc
     3421 gtaactgtat cgctattctc cacggagaca accaaacaga agttcttgaa aaaggtcctg
     3481 aagtattgga acaggaaggt caagactttt tggaacattt caaaaaactc ttggagtcag
     3541 ttgaagtagt ggccatctca ggtagtctgc cagctggcct tccagttgat tactatgcga
     3601 gcttggtaga acttgctaat caagctggca agcctgtagt cttggactgc tcaggtgcag
     3661 cacttcaggc tgttcttgaa tcaccccata aaccaacagt catcaaacca aataatgaag
     3721 aattgtctca gcttcttgga agagaagttt ctgaggattt ggatgaatta aaagaagtac
     3781 ttcaagaacc tttgtttgca gggattgaat ggattatcgt ttcacttggt gccaacggta
     3841 cttttgccaa acatggtgac actttctaca aggtagatat tcctagaatt caggtggtaa
     3901 atcctgttgg atctggagac tctactgtgg caggaatttc ttcaggactt cttcacaaag
     3961 aatcggatgc agaattactc atcaaggcaa atgtccttgg tatgctcaat gctcaagaaa
     4021 aaatgactgg tcatgtcaac atggccaact atcaagttct atatgatcaa ttaatagtaa
     4081 aagaggtata aaatggcttt aacagaacaa aaacgtgcac gcttagaaaa actttctgat
     4141 gaaaatggta tcatctcagc tcttgcattt gaccaacgtg gtgctttgaa acgcctcatg
     4201 gctcaacacc aaacagaaga accaactgtg gctcaaatgg aagaactgaa agtcttggta
     4261 gcagatgaat tgactaaata cgcttcatca atgcttcttg accctgagta tggacttcca
     4321 gcaactaaag ctcttgatga aaaagctggt cttctccttg cttatgaaaa aacaggttat
     4381 gacacaacaa gtacaaaacg cttgccagac tgcttggatg tttggtctgc aaaacgtatt
     4441 aaagaagagg gtgcagatgc agttaaattc ttgctttact atgatgtaga tagttcagac
     4501 gaactcaacc aagaaaaaca agcttatatc gagcgtatcg gttctgagtg tgtggctgaa
     4561 gatatcccat tcttccttga aatccttgct tacgatgaaa aaattgcaga cgcaggttct
     4621 gtagaatatg cgaaagtaaa accacacaaa gttatcggtg ctatgaaagt cttttcagac
     4681 ccacgcttta acattgatgt cttgaaagtt gaagttcctg ttaacattaa atatgttgaa
     4741 ggcttcgctg aaggtgaagt ggtttacaca cgtgaagaag cagcagcctt cttcaaagcg
     4801 caagatgaag caacgaactt gccatacatt tacttgagtg ctggtgtatc agctaaactc
     4861 ttccaagata ctcttgtatt tgctcatgaa tcaggtgcaa actttaacgg agttctttgt
     4921 ggccgtgcta catgggcagg atcagttgaa gcttacatca aagatggtga agcagcagct
     4981 cgcgaatggc ttcgcacaac tggatttgaa aacattgatg agctcaataa agttcttcaa
     5041 acaacagcga cttcatggaa agaacgtgtg taagaaagtc ctcctagttt aggaacatga
     5101 atctaaaaaa atttaaaaaa agttgtatgt aaaggtttac aaaataactt acttgtgcta
     5161 tacttaaatc acaagttaat acaaggtgag tgttactaag taatattagg catgatcaca
     5221 ggtgaattag aaatcagctg attttctagt tcatttgtgg tcattttttg tactcatata
     5281 cttttaagat ataaaaggag gttgacatgt atcgaattct aaatccaatg aatcacaatg
     5341 tctcgcttgt cagaaatgat aagggagaag aggtgattgt aattggtaag gggattgcat
     5401 tcggaaagaa gaagggggat ttgattgctg aaaatcaggt tgagaaaatc tttcggatga
     5461 agaccgaaga gtccagagaa aactttatgg ctcttctcaa agatgttccg cttgatttta
     5521 tcacagtgac ctatgaaatc attgataagc tatcaaagaa atatcattat ccgattcaag
     5581 agtatctcta tgtaaccttg acagatcata tttactgttc ttatcaagct ctaactcaag
     5641 gaaggtacaa ggatagtaat ctgccagata tttccgctaa gtatcctgtc gcttttcaaa
     5701 tcgcaaatga agcttttgaa atttaccgtc agaagctagc agatcatttt cctgaggacg
     5761 aaattattcg gattgcttat catttcatta atgctgaagg tgaaaatgaa gtggaacttg
     5821 tggagtcgat tgataagagg aaagaaattc tcaggaatgt tgaagaagtt ttaacggact
     5881 atgcaattca acgaactaaa aagaataacc atttctatga tcgctttatg atccatttga
     5941 attatttctt ggattattta gacagatcta gagatgataa ccaatcactt ctggatatgg
     6001 aagatcatat taaacaatcc tatccaaaag ccttcgagat tggttccaag atctatgatg
     6061 tgattacgca acatacgggt cttgatttgt ataaaagtga acgagtttat ctagttctac
     6121 atatccaacg tttattgtca taaaaa
//
LOCUS       DAVKAO010000085         6048 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKAO010000085 DAVKAO010000000
VERSION     DAVKAO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.085"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            168..452
                     /locus_tag="VWX16_001834"
                     /pseudo
     CDS             168..452
                     /locus_tag="VWX16_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            661..1320
                     /locus_tag="VWX16_001835"
     CDS             661..1320
                     /locus_tag="VWX16_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9159136.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIYMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAKLTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1345..2412
                     /locus_tag="VWX16_001836"
     CDS             1345..2412
                     /locus_tag="VWX16_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9159137.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            2515..3525
                     /locus_tag="VWX16_001837"
     CDS             2515..3525
                     /locus_tag="VWX16_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9159138.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3537..5228
                     /locus_tag="VWX16_001838"
     CDS             3537..5228
                     /locus_tag="VWX16_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9159139.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5231..5941
                     /locus_tag="VWX16_001839"
     CDS             5231..5941
                     /locus_tag="VWX16_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9159140.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2023 a          839 c         1155 g         2031 t
ORIGIN      
        1 tatactatat tatcgcttac caacatttgc ttttatattt gctaaacttt cagaaaaaaa
       61 gttggagtat atgatgctag gtgactgcgt catgttagta aacgaaatgg aaataactga
      121 tcatagagta gataacttat ttgaaaaagg gaaaaatgag ataaaagatc catgtagtag
      181 atttagcaat aggacatata aaagctttag aaaaagtatc tgaaaaaaca gatgtttata
      241 tctataacct atgctcggga gaaggcacta gtgtattaca acttgtaaat acatttgaaa
      301 gtgttaataa gatccctatt ccttataaaa tagttccaag acgttcagga gacgttgcaa
      361 cttgttatgc aaatgcagac aaagcatata aggaattaaa ttggaggaca acaaaatcga
      421 ttgaagacat gtgtagagat acatggaatt agcaatcaaa aaatcccaat ggctataatt
      481 gatgatactg tctatatgag tgctgtattt tggaaaaatt acttaacctt ttaatgaaat
      541 gcatatttaa tgaaagataa attaaaaaaa atgtttgaca gcgcttgaca aaggaaacgg
      601 tttcattgta taatgcaaac atgtgacacc gatgtcaaat ataaaaaaga aggtctttat
      661 atgaaaaact taacaaaaat taagttcaaa gagaatgggg aatttaatca tttccctggg
      721 aatacagttg tagcaaatct ttatactaaa caagatttga tggaagttgt tgatattatt
      781 caatcacgtt atagagaatt accatttatc gataagttta ctttaactcc aaggaattct
      841 attcacatga ctgtaattga attgttgtgc catgaaaatc gtgaaacgga gttttggagc
      901 agtaatcttc ctctagatac acctttacag gaaatacatg attactttgc taaacaactt
      961 gaaatttttc cattgttgga tgaagaaatt tatatgcgta taactgaaat gggaaaacaa
     1021 aacatactag ttgaacctgc agatgaagct tctgcaaaga gattagaaga aattcgtact
     1081 tatgtctcag aaaaagcagg tgtttgtttc cctaatcatg atagatatca attccatatt
     1141 tcaattgggt atcttcggat tcctctaacc gaagaggagg aagaagagtt tactaaagtc
     1201 agagcaaaat taactgaaat tttattagag aagattccaa ctattactgt aaaccgtatt
     1261 gactatactg tatttgaaga tatgagacaa tttgttccat atcacgaaaa atttaaataa
     1321 aataataaaa aaggagaact tatcatgaaa aaaatgaaag tttggtctac tgtacttgca
     1381 acgggagttg ctcttactac acttgctgct tgctctggag gttcaaattc tacgactgct
     1441 tcttcatctg aagaaaaagc tgataaaagt caagaattag ttatctattc gaactcagtc
     1501 tcaaatggtc gtggtgattg gttaactgct aaagcaaaag aagctggttt taatataaaa
     1561 atggttgata tcgctggcgc tcaattagca gaccgtgtta ttgctgagaa gaataatgca
     1621 gttgcagata tggtatttgg aattggtgct gttgactcaa ataaaattag agatcaaaaa
     1681 ttactagtac agtacaagcc taaatggtta gataaaattg atcaatcttt atcagataaa
     1741 gataattatt ataatcctgt gattgttcaa ccattagttt taattggggc gcctgatgta
     1801 aaagaaatgc ctaaagattg gactgaatta ggtagtaagt ataaaggtaa atattcaatt
     1861 tatggtcttc aaggtggtac aggacgggca attctagcaa gtatcttagt tcgttacctt
     1921 gatgaaaaag gagatttagg cgtatcagaa aaaggatggg aagttgcaaa agaatacttt
     1981 gcaaatgcct atactcttca aaaaggtgaa agctcagttg ttaaagcgtt agataaagaa
     2041 agcccaattc aatacggtat gatctggggt tctggagcgt taattggaca aaaagaacaa
     2101 aatgttgaat tcaaagttat gactcctgag attggtgtac catttgtaac tgaacaaact
     2161 atggttttaa gcactagtaa aaaacaagcg ttagctaaag aatttattga ttggtttggt
     2221 caatcagaaa ttcaagtaga atatagtaaa aaatttggct ctatccctgc caataaagat
     2281 gccctaaaag agttacctga agatacgaaa aaatttgttg atcaagtgaa accacaaaat
     2341 attgactggg aagctgttgg aaagcatttg gatgaatggg tagaaaaagc tgaattagaa
     2401 tacgtaaaat aagaaataag agttattttt tatgtccaag tctctctatt atttctaaat
     2461 ttaaggaaga aataaagaga ggcttttggt ttagtaatag agggagttag tgagatgatt
     2521 aaatttgata atattcaaat taaatatggt gattttgttg caattgataa tctgaattta
     2581 gatatacatg aaggggaatt ctttacattt cttgggcctt caggatgtgg taaatcaact
     2641 actttgagag cattggtagg ttttttagat ccatcatcag gaagtattga agttaatgga
     2701 acagatgtca ctcatttgga acctgaaaag cgtggaattg gtattgtatt tcaatcttat
     2761 gcgctatttc caactatgac tgtttttgat aatattgcat ttggtttaaa agttaagaag
     2821 gtagctccag atgttattaa agctaaagta tcagcagtgg cagcaaaaat taagatctct
     2881 gatcaacagt tacagcgtaa tgtatcagaa ttatctgggg gtcaacaaca aagggtagca
     2941 ttggctcgtg ctctggttct tgaacctaaa attctttgtc tagatgaacc attgtcaaac
     3001 cttgacgcaa aattacgtgt agatttgaga aaagagttga aaagacttca aaaagagtta
     3061 ggtattacta ctttatatgt tactcatgat caagaggaag ccttgacttt atctgataga
     3121 attgcagtct ttaacaatgg atacatcgaa caggtcggta caccagtaga gatttatcat
     3181 aattctcaaa ctgaatttgt atgtgatttt attggagata ttaatgtttt gaccgatgaa
     3241 acagtccacg aagtattatt gaaaaataca agcgttttct tagaggataa aaaaggatac
     3301 attcgattag agaaagttcg attcaatcgt gaaactgaac aagattttat tctaaaaggg
     3361 acaattattg atgttgagtt ttctggagtt acaattcact atacaataaa agtttctgaa
     3421 agtcagattc ttaatgtaac aagtattgat agtcaggctg ctattagatc tgtcggagaa
     3481 agtgtggaat tatttatcac accatcagac gttctgcaat tttaaggagg tgcagtatgc
     3541 gtcataaatt aaatttaaaa gattggctta ttcgtttagg gttaatctgg ttcttagtaa
     3601 catttattat ttatccaaac tttgatctag tagtgaatgt atttgtaaaa ggaggagaat
     3661 tttcccttga tgctgtacat cgtgttctaa aatctcagag ggcacttcag agtattatga
     3721 acagttttaa gttagcattt tcactcatta ttacagttaa tgtcgtaggt attctttgtg
     3781 ttctatttac agagtacttt gatattaaag gtgctaaaat tttaaaatta ggttatatga
     3841 cctctttaat ttatggagga gtggttttag cgactggata taaatttgtc tatggtcctt
     3901 atggattgat tacaaaattt ttacaaaatg ttatcccttc tttagactct aactggttta
     3961 ttgggtatgg tgcagtctta ttcattatga cattttcagg aactgctaat catacattgt
     4021 ttttaacaaa tacaattcga agcgttgact atcacactat tgaggctgct cgaaatatgg
     4081 gagcaaaacc atttactgtt ttccgaaaag tagtgttacc aaccttaatt ccaactctat
     4141 ttgcacttac tattatggtt tttcttagtg gtttatctgc agtagcagca cccatgattg
     4201 ttggtggtaa agaatttcaa actataaatc caatgattat tacatttgca gggatgggga
     4261 attctcgtga tttagctgcc ttacttgcaa ttattttagg tattgcaact acaattttgc
     4321 ttactatcat gaataagata gaaaaaggtg gaaattatat ttctatctct aagactaaag
     4381 cgcctcttaa aaaacaaaaa attgcgtcta agccttggaa tatcattgct cacattgtag
     4441 catatggatt gttcacagtt ttcatgcttc cactaatttt tatagtatta tactcattta
     4501 cagatccagt tgcaattcaa acaggtaact taacattatc aaactttact ttagaaaatt
     4561 atcgcttatt ctttagtaat agtgcggcat tctctccatt cttggtcagc tttatttatt
     4621 ctattattgc tgcgacaaca gcaacaattc tcgcagttgt atttgctcgt gttgtcagaa
     4681 aacataaatc tcgttttgat ttcttatttg aatatggtgc tctacttcct tggttactac
     4741 caagtacact tttagcagta agtttattat ttacttttaa tcagccacaa tttcttgtct
     4801 tgaatcagat tttggtaggt agtttggtaa ttctacttat tgcatatata gttgtaaaaa
     4861 tcccattttc ttatagaatg gtacgtgcta ttttatttag tgttgatgat gagatggaag
     4921 atgcagcaag aagtatgggt gcttcacctt tttatactat gatgaaggtt atcattccat
     4981 ttattttacc ggttgttctc tctgttattg ctttaaactt taactcttta ttaactgact
     5041 tcgacttatc tgtattcctt taccatcccc tagctcaacc attaggtatt acgattcgat
     5101 ctgcaggtga tgaaacagca acatctaatg cacaagctct ggtatttgtt tatacaattg
     5161 ttctgatgat tatttctgga acggtattat acttcacaca aagaccgggg cgtaaagtaa
     5221 ggaaataatc atgacagcca ctagtcttgg gttatcaaat attgaaatag ttgtcaggat
     5281 tgttttatca gtagtcattg gtagtataat tggtttagag agagggagca aatcccagcc
     5341 tgcaggcatc cgaacttata gtattgtttg tctagctgca tgtttgatta tgatgacgaa
     5401 tgaatacgta tcttataaat ttgggacagg agatcctaca cgattaggag ctcaagttat
     5461 atcaggtgtg ggttttctag gcgctggaac gattcttatt acagataaaa agaaaattac
     5521 aggtctgaca actgcagcag gcatttgggc ttcggcagga attggattag ctattggagt
     5581 aggtttttat gagggagctc ttttagtagc tatttctgtt tggggtgtga tatccatgtt
     5641 ccaaccacta aaaaaatatc tgcaaaatcg ttctaaaatg attgaattgt atatagtggt
     5701 taaatctaca gaagcctata atcgtgtatt ggtttattgt gcagaaaatg gtatcagaat
     5761 gaccgattca agaacagcgt ttggagatgt taattctgac agaattgaat actttgatgt
     5821 tccagataaa aaaatagcgt catttattac tctggaatta tcaggtagat ttgaacatct
     5881 tcgactgatg gaagaaattg ctaatattgt aggtgttatt tatgtcgaag aaattagctg
     5941 aaatttagta aacaaaggga cagatgtttt tgtccttttg ttttttagat gttttaatca
     6001 tctaattttt gagtagattt ttgctcagag gtttttatga aagattat
//
LOCUS       DAVKAO010000086         6044 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKAO010000086 DAVKAO010000000
VERSION     DAVKAO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.086"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..743)
                     /locus_tag="VWX16_001840"
     CDS             complement(<1..743)
                     /locus_tag="VWX16_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEU9159141.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDINFTYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLF"
     gene            complement(745..1302)
                     /locus_tag="VWX16_001841"
     CDS             complement(745..1302)
                     /locus_tag="VWX16_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9159142.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEIAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLETRI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            complement(1289..1738)
                     /locus_tag="VWX16_001842"
     CDS             complement(1289..1738)
                     /locus_tag="VWX16_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU9159143.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDIKGSFIKKARKKDGKN"
     gene            complement(2150..2545)
                     /locus_tag="VWX16_001843"
     CDS             complement(2150..2545)
                     /locus_tag="VWX16_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9159144.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(2641..2895)
                     /locus_tag="VWX16_001844"
     CDS             complement(2641..2895)
                     /locus_tag="VWX16_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9159145.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(2905..4239)
                     /locus_tag="VWX16_001845"
     CDS             complement(2905..4239)
                     /locus_tag="VWX16_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9159146.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYEYMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(4239..4472)
                     /locus_tag="VWX16_001846"
     CDS             complement(4239..4472)
                     /locus_tag="VWX16_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9159147.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            4503..4580
                     /locus_tag="VWX16_001847"
                     /pseudo
     CDS             4503..4580
                     /locus_tag="VWX16_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4979..5161)
                     /locus_tag="VWX16_001848"
     CDS             complement(4979..5161)
                     /locus_tag="VWX16_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159148.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEDKK"
     gene            complement(5163..5942)
                     /locus_tag="VWX16_001849"
     CDS             complement(5163..5942)
                     /locus_tag="VWX16_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159149.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         1779 a         1362 c         1049 g         1854 t
ORIGIN      
        1 aaaaacaact tattataatt accatagagc tctgtaagag cattaaagta ttctttcact
       61 agagctatag cgtcaatctt agatgtttct tggtttagtt ttatcaactc ccctttcaat
      121 tttttccatt taaagttgga atttaaaaga taatctaaat caatatagaa atctattata
      181 acagtctcat catctgggct aacagctttt acctcattta ttggtaagtc agtatgttgc
      241 gaataatttc tcaaagaatg gcaaaatcga taagtaaaat tgatatcata ctgatagctt
      301 gcaaaaccat gtatacaatc aaactcctca ctatctttgg aatatttttg tttaatttgg
      361 ttttcgttaa aatcgacaaa caattttcca ctggataata aatgaatcat gaaacgattt
      421 acatctgcct cttcaatttc taattccttt gactcaagtt tatttttaat acttaaaaaa
      481 tccgagacat ctgaaataaa caattcgaac atattcttga ttttttgaaa attcttttga
      541 atctcaaaaa tttggtccga gactgacatt atttccttgg ttggttcaat atcagagatg
      601 acaatacgtt tttcattgtt gttaatttct aaataagcta atcgctcgat aatatcacta
      661 tcatttaatt cagcatactc aacatcaatt tggcgtaact gcttctcaga tagagagctt
      721 acttcctctt ttacatcgct catcttattc ctcccactcg ctcttcagca agccatatat
      781 cagactgtca cagcggtttc cttgaacatc ctttcggtct cttatgcgag tttcgagggt
      841 aaagccaagc ttttccgcga ctcgtttact ttgaaggtta tatccaaagc aagttagttc
      901 aatcttgtga agacccaaat ctttaaaggc taagtcaatc aaggcacgcg cagcttctgg
      961 cacataacct cgcccccaat agtctgggtg taaggtatag ccgatttcca gcacatcatc
     1021 ttcatggcga tggttgaaat cgacagagcc aacgatctta tccgttcctt tgacgacaat
     1081 cccgtagcct gctgggagat tttccttttg attacgctcc ggaagaatgt gctccagata
     1141 ataaatctca tcttccaagg tcttgactgg aggaaaacct gctggatagg cgatctctgg
     1201 caaactagca tagtcaaaga tatcctcagc atccgccaca gtacggactc gtaaaacgag
     1261 acgttctgtt tcgattttat ctggcagctc agttcttgcc atccttcttc ctcgcttttt
     1321 tgatgaaact gcccttaata tctacacgct tatccagata gcgataaacg cgctgatatc
     1381 catctcccat gaaataggtt ggggcaaaca gttgattttt aaaatgtccc ttttcatcca
     1441 ggagttctgg ggcaacaagt cgctcaagaa tcttggcaaa gatgtggcaa ataccgtctt
     1501 cctcaacaat cctatctacc cgacaatcta aaacaagtgg acaggcgtct aaaataggaa
     1561 tctgagttcg ttcagaaatt tcataatgca ctcccaaacg ttccaatttc tcctgatgac
     1621 tgataaaacc agcctgctcc atcgcaagca tagaagtttc atcagaaata ttcacagtaa
     1681 atttttgata ctgtttgatc tgctctgcgg cattctctct cgcaacgact ccaatcacaa
     1741 cccaatctcc tagactataa gaggaactac aggtcgtgat gttatagcca aaattctaat
     1801 cttgatatcc taaaataaaa acaggaaaac cataatatag tttattagtt ttaaatgatt
     1861 gttttataac aacctccttt gactaagaca caaaagaaaa gccctgccat ctacatgaca
     1921 gggacgaatg tgtttatccg cggtaccacc caatttcggg ccaagcccgc atctttagtc
     1981 tttaaaataa aaagacagaa atatttcatt ttttcagtca ttagcaacca gttttgacac
     2041 atttgtggac ttctcagcgc cgccactttc tgtaaaatgt gggattcaaa acacctctaa
     2101 tggctctatt gtagcaagtt tttctcggaa atgcaaggct caagaaaaat tacttgaact
     2161 tgatgccatt ttctttcaat ttcttgatgg tagctgggcc gattcctttc aaggcaagga
     2221 gttctttttc agtccagttt ttgaaatctg aagcagactt gattccttca tcatagaaag
     2281 tcttggcacg atcaagtgga agtccaccca agtttgctgc aaaatcttct acagaattag
     2341 ctactttttg agcttgctct ttggtagctt ctactgcttg ttcaactttt ttagaaacag
     2401 ccttaccagc tgcgcttgct gactgttctg cacgcttcac gacttttttt gtctcatcta
     2461 caaccttagt cacagtactt gaaaaggcac ctgcgcgacg gaggccattt cgtaattgtt
     2521 ttttacgatt gagtttcttt gacatgataa aatcctttca ataaaaaatc cctgctctga
     2581 caaggattta acataaacat tctatcaaga ttttagtgaa aaatcaagaa tctttctcgt
     2641 ttaataattt cgctcaccaa acaagccttc tggcaaatca tggaatccct tgcctgcttt
     2701 gaagttttca aacttaccaa gcaagaactg ataagcatcc aagcgactct cgtagcgaat
     2761 catggcatct ttggcacgct cgtcctggtc ttcttcgtaa actttttgac tttccttgtg
     2821 cgccatgtcg ttaatcatga tttgggtatt gacccaagct tcaaattcct tcataaattc
     2881 ttgttcgtaa ctcatttttt ctccttattc taatccacga aaatagtccg tatcaatctc
     2941 acggtagggc tggatatcct gaacatactc caacactcct tggaattctc cgtcttcatc
     3001 gtgtactgca gcataggtga tgtggacaaa cttacctcgc gactcagact tgaaccacat
     3061 ttcatacttg tccttggtcc cctcacgaag ccccttcatg atagttttga ccttgtccaa
     3121 gtacttaggc ggatggcaga gttcgacatt gcgcccgact tgagacggcg tccgtttgaa
     3181 aatcatctca tcagctggcg tattgtcatt gtaatactgg aaaatatcat ctttattgac
     3241 aaaggtaatc tccataggga ggtgattgag gatgagattg gcctgctcga ctgaaagata
     3301 gccattacca aaagcctgtt gactatggcg gtccagcaca gcttcctttt ccttaggggt
     3361 aaaggtaatg gtaaaatggc cttctggcgt atctatgact tgttgaactt gaccttctgc
     3421 cgtatctagc tgtacaggct cctctgcaat cttttcctca ataaagctct gtcgttctgg
     3481 cacccatttc tctgacggac ggatgatggc atagccatag gcatcgctct cctccgcaat
     3541 ctgaagccag tcatcctgag taaaagactc aaggagaatc atgaggagga tggactcttc
     3601 cttgaaaatc atactttcaa actctgtcgc aaaagcttca aaagcttcct ttacactgct
     3661 aattgacact tctggtagtg acttggctgt cgttagagct gtttgaaaga gttccctaat
     3721 ctgatcatcc actccccaca taactttggg aggtgaatcg tgtccatagc gctccatgat
     3781 aggaaagaag agttcttcct tacgttggta atggatgtca aattgaccca caagtcccat
     3841 ctgacgcacc aaacccttac gcatctccgc cagcatttcc tcgtcttcca tatactcata
     3901 ggtatctaac aatctacgaa tgcgaatcaa ggccgcacgg agagccagat tttcttcttt
     3961 gaagacacga actgggtgac ctggatgctc agtatctgaa acttcgacac ctttgatagc
     4021 atttttaaaa agattggcat ggacatcaca gagttccata acatcttcaa aggtgacacc
     4081 tgagtctgag ttcatcagct cgtgctccat aagggaaatc tcgatggctg agacacccgc
     4141 aaaggtcgca tcaaagcgct cttgaaccga ctcaggagag gcgccattgt gcaattctaa
     4201 caaaatatcc cgtaggacat gaatccgttc atctgccatt agtctaatcc aatcacttcg
     4261 tagccattcg cttccagtgt gcgtacaatc ttgtccatag gagttcctgc tagcttagaa
     4321 ccctgtttaa gtgatacttt acgaccaact gtattgcgca ttaagggatt ggcaaggggt
     4381 ttaaaaccca actccactag aatttccaag acttctggat gcttgtccac cacttctgca
     4441 acaggaattg acacatcgat gatattgtcc atgacgacct ccattgtatg ttctaatact
     4501 caatgaaaat caaagagcaa actagaaagc tagccgcagg ttgctcaaaa cagtgctttg
     4561 aggttgtaga tagaactgac gaagtcagct caaaacaccg ttttgaggtt gtggatagaa
     4621 ctgacgaagt cagctcaaaa caccgttttg aggttgtgga tagaactgac gaagtcagta
     4681 acatatatac agcaaggtga agctgacgtg gtttgaagag attttcgaag agtataaaat
     4741 gattttactg cttatattat accatatgga aagagaataa aaaactagca gtggagttcc
     4801 tgctagttct ctgcgacttg gattaccatc tagataagtt taaagagcca atccaatatc
     4861 ttaaacacta tcttgtaaaa cagcaattgg actgcaaaag agatgatcat ccaaaagaat
     4921 ttcacaatcc caataatcgg tttgataaaa ataatggcaa gaagtcccca gaaaaatttc
     4981 attttttatc ctctggctta atgagccacc gagccgtatc cattagctta tctgcaataa
     5041 agagttttcc cagaccaaca acgacatggt catctttctt tatcgttttc attactttta
     5101 caccttgcac ggtcaaaaag taattaccat aaattttagc tagagttttt atgagtccca
     5161 tattactctc cttcgatgat ttcagcctct tttcggattg tttcaacatc ttcttgatat
     5221 tgaacttcac tatagttaac tagcttggac aattctttct gcaagctttc acccatcggt
     5281 ttcatagttg caaaggttaa accacctgaa atgataccac ccaagatagg aataaatttc
     5341 cccattcctt tggccagccc tcccttggta agattcacac caaatattct taagactttc
     5401 ttcaaaatag gataccaaag cgtctttgtt aaagctttat taggtactgt tttcattacc
     5461 tgtttggcaa ttgttatacc accagcacgt agcaaagcag cagttccatt cacccccaac
     5521 atgacgccta gataaagcaa aagggtattt tgagcatctt cactcaactc atcgcgtgaa
     5581 gcccaaagat cctcataacc ataaatataa cctaattctt gggccaattt cagagagaaa
     5641 ccataaaatt gagccacatc agctggaatg gtaattgcca tagcaagccc tccaggtaat
     5701 cctgccaaaa cagaagtccc actcgctaat aaaacattat ccctaataca ggcattagcc
     5761 actcgatcta agatttcttg agatagaaga gtcgttggac cttgttccaa taaggtagga
     5821 atatcttttg gatccaattc tttggaaaac ttatccacta aaaatttcga acgatcaacc
     5881 ttaacaactg gtagttttac cacttgttgt aatacttgca tagctaagtc ttgttcagcc
     5941 atatacaaac tccttatttc ttttttacac cttatcatat tatatttcct acaaaaaaaa
     6001 gcattttcta attagataac attaaaaaat acagaatccc tttt
//
LOCUS       DAVKAO010000087         6032 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKAO010000087 DAVKAO010000000
VERSION     DAVKAO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.087"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            170..370
                     /locus_tag="VWX16_001850"
     CDS             170..370
                     /locus_tag="VWX16_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159150.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            395..811
                     /locus_tag="VWX16_001851"
     CDS             395..811
                     /locus_tag="VWX16_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159151.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1042..1749
                     /gene="ccdA2"
                     /locus_tag="VWX16_001852"
     CDS             1042..1749
                     /gene="ccdA2"
                     /locus_tag="VWX16_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9159152.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1760..2317
                     /gene="sdbB"
                     /locus_tag="VWX16_001853"
     CDS             1760..2317
                     /gene="sdbB"
                     /locus_tag="VWX16_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9159153.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2690..4081
                     /locus_tag="VWX16_001854"
     CDS             2690..4081
                     /locus_tag="VWX16_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9159154.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLILLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4224..5141
                     /gene="adcAII"
                     /locus_tag="VWX16_001855"
     CDS             4224..5141
                     /gene="adcAII"
                     /locus_tag="VWX16_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9159155.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQAGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDSDVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQSKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLEADPQNDKTYLENLEENMSVLAEELK"
     gene            5149..>6032
                     /locus_tag="VWX16_001856"
     CDS             5149..>6032
                     /locus_tag="VWX16_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9159156.1"
                     /translation="MKINKKYVAGSVAVLALSVCSYELGRYQAGQVKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEH
                     SQHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPK
                     NELSASELAAAEAYWNGKQGSRPSSSSSYNANTAQPRLSENHNLTVTPTYHQNQGENI
                     SSLLRELYAKPLSERHVES"
BASE COUNT         1839 a         1083 c         1290 g         1820 t
ORIGIN      
        1 gtaaaatgaa ataagaacag gacaaatcga tcaggacagt aaaatcgaat tctaacaatg
       61 ttttagaagt ctatgtgtac tattctagtt tcaatatacc ataactgctc ctagtcaacc
      121 tgcaagtcct gtcccagctc caagctcagc tccgattcaa gatcctagag tggtagcaag
      181 cgcttcggct agctcaacga gcacacaagc tcaagagcaa gttgacaagt ctgaacttcg
      241 tgccttgagt caagagttag accaacgctt gaaagctttg gcaacagtat ctgatccaaa
      301 aatcgatgca accaaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt
      361 gacagagtaa ggtctgcata ctgcagtaga gtctgtgaag gctgccttgg actctctcaa
      421 agatgtgaaa gcaaatgcta gcgacagcaa gcctgcacag gacaagaagg atgcaaaaca
      481 aggaacggaa gatagtaagg attcagataa gatgactgaa acaaactcag ttccggcagg
      541 agtgattgtg gtcagtctac ttgccctcct aggcgtgatt gccttctggc tgattcgccg
      601 taagaaagag tcagaaatcc agcaattaag cacggaattg atcaaggttc taggacagct
      661 agatgcagaa aaagcggata aaaaagtcct tgccaaagcc caaaaccttc tccaagaaac
      721 ccttgatttc gtgaaagaag aaaatggctc agcagagaca gaaactaaac tagtagagga
      781 gcttaaagca atccttgaca aactcaagta aaacatgatt tatgaaatgt cagacctaaa
      841 agacattttc gttcacaaaa gcagtgacga gtaatccttg tcgttgcttt ttggctattt
      901 tgggggataa gatccgcctt gactagcaaa agaaaagatt ggtccaatag tgcccgatac
      961 gattttgcgt agcagaaagc ttacaaataa aattgaatat agtaaaatag gattagaatt
     1021 attaagaaag ttggttcttt attggaaacg atagtatttt taatctctgt ttttctagca
     1081 ggtgttttat cctttttttc tccttgtatt tttcctcttc tgccagtcta tgctgggatt
     1141 ttattggatg atcaagaaag tgcaaaaagc ttttctttgt ttgggagaaa ggttctctgg
     1201 tcaggcttga ttcgaacact ttgctttatc gctggtatct ctctcatttt ctttattcta
     1261 ggctttggtg ctggttactt tggtcatatt ctctatgcaa attggtttcg atatgtcatg
     1321 ggagctatta ttatcatttt gggtcttcac cagatggaaa tttttcattt gaagaaatta
     1381 gaagttcaaa aaagttttac ctttaaaaaa tcagattcta atcgttattg gtcagctttt
     1441 ttacttggta ttacctttag ctttggttgg acaccttgta ttggtccagt tttaagttct
     1501 gttttagcac ttgcggcttc tggaggcaat ggcgcttggc aaggagcgat ttatactctc
     1561 atttacactc tgggcatggc ccttcctttc ttggtattgg cactagcttc aggtctagtc
     1621 atgccatatt ttagtaaaat caagcgtcat atgatgctac taaagaaaat tggtggtttc
     1681 ctcattgttt taatgggaat tttgttacta ttaggacaag taaatgttct agctggaatt
     1741 tttgaataaa ggaggacaaa tgaaaaaagt aatgtttgct ggcttaagtc tcttgtcatt
     1801 agttgtattg atggcctgtg gtgaggaaga aactaaaaag actcaagcag cacaacagcc
     1861 aaaacaacaa acgactgtac aacaaattgc tgttggaaaa gatgctccag acttcacatt
     1921 gcaatccatg gatggcaaag aagttaagtt atctgatttt aagggtaaaa aggtttactt
     1981 gaagttttgg gcttcatggt gtggtccatg caagaaaagt atgccagagt tgatggaact
     2041 agcggcgaaa ccagatcgtg atttcgaaat tcttactgtc attgcaccag gaattcaagg
     2101 tgaaaaaact gttgagcaat tcccacaatg gttccaggaa caaggatata aggatatccc
     2161 agttctttat gataccaaag caaccacctt ccaagcttat caaattcgaa gcattcctac
     2221 agaatattta attgatagcc aaggaaagat tggaaagatt caatttggtg ctatcagtaa
     2281 tgcggatgca gaagcagcat ttaaagaaat gaactagaat cgataaaatc tgattacaag
     2341 atgtagtcag attttggtaa cttctaaaac taacttccag tttcccacaa cctagctttt
     2401 agtatgagaa aaacgcgtga catcagtgga aatccgtctt agactaactt ccactggttt
     2461 tctttttctt gatatataag ggttcaaaag cgaaaagact cagaaaaagt gcacgacaaa
     2521 tagccctaaa acagagtcgt cttagagtaa cttccagttt tgggagagag atggaagtta
     2581 ctttgagaag ttacggtagt cagatttttt agaaaaacat tttataaata ttcacatatc
     2641 tcctatattt atggtaaaat agaattatca gtttattttg gagtcaaaga tgaatatatt
     2701 tagaacaaag aatgttagtt tagataaaac agagatgcat aggcatttga agttatggga
     2761 tttgattttg ctgggtatcg gagccatggt agggacaggc gtctttacaa tcacaggtac
     2821 tgcagctgca acacttgctg gcccagccct agtgatttca atcgttattt ctgccttgtg
     2881 tgtgggatta tcagccctct tttttgcaga atttgcctcg cgagtacccg ctacaggagg
     2941 tgcctatagt tacctctatg ctatcttagg agaattccct gcctggttgg ctggttggtt
     3001 aaccatgatg gagttcatga cagccatatc aggcgtagct tcgggttggg cagcttattt
     3061 taaaggcttg ctctctcaat acgggatagc ctttcctcag gctttaaatg gtacctttaa
     3121 tcctcaagca gggacatttg ttgatttatt gcctattctt gtcttggtct tggtgacttc
     3181 gcttgtttta ctaaatgcca aggcagcctt acgctttaat tcgattctag ttattttgaa
     3241 attttccgct ttagctctct ttgttttagt aggaatttgg aatattaagt ttgataattg
     3301 gagcaatttt gctccttacg gttttggaca aatctatgga gctagcactg gtattatggc
     3361 tggtgcgtcc ttgatgttct tcggtttttt gggatttgaa tccatctcta tggctgtaga
     3421 tgaagtcaag actcctcaaa aaaatattcc tagagggatt gtcttatcgc tttctatcgt
     3481 aaccattctc tatgccttgg tgacccttgt tttgactggt gtggttcact atagtcattt
     3541 aaatgtcgat gatgccgttg cctttgccct tcgtagtgtt gggattagtt gggcagccaa
     3601 ctatgtgtca ttagtggcta tcttgacctt gattacagtt tgtatttcga tgacctatgc
     3661 cctatcgcgt atgatttaca gtttagcacg tgacggctta gtgcctgctg cctttaagga
     3721 actgacgaag actagcaaga taccaaagaa tgctactatc ttgacaggtc tagcttcagc
     3781 agtagcagca ggaatgttcc cactagccag tattgcagcc tttttaaata tttgtacctt
     3841 agcctacttg atcctgctgg cttatggtct gattcgctta cggaaagaaa aaggaatgcc
     3901 caaagctggg gaatttaaaa caccattggt acctctgtta ccaattttat cgatcatcat
     3961 ttgtttatcc ttcatgttac agtataatat gaatacctgg attgctttcc tagttgcgtt
     4021 gttagtagga agtattattt acttcactta tggctataag cattctacca tagaagaata
     4081 aagtgaaagt ctattgagtt gttgacactc agcagatttt tatatgtgaa agaaatttca
     4141 aattttttct aaaaatatct tgacagatta aattttcagg agtagaatat ttactagtta
     4201 attaaatggt taaggagttg ttcatgaaga aacaaaattt atttttagtc ctgttaagtg
     4261 tctttctttt gtgcttgggg gcttgtggtc aaaaggaaag tcaggcagga aaggggatga
     4321 aaattgtgac cagtttttat cctatctacg ctatggttaa ggaagtatct ggtgacttga
     4381 atgatgttcg gatgattcag tcaagtagtg gtattcactc ctttgaacct tcggcaaatg
     4441 atatcgcagc catctatgat tcagatgtct ttgtttacca ttctcataca ctcgaatctt
     4501 gggcaggaag tctggatcca aatctaaaaa aatccaaagt gaaggtctta gaggcttctg
     4561 agggaatgac cttggaacgt gtccctggac tagaggatgt ggaagcaggg gatggagttg
     4621 atgaaaaaac gctctatgac cctcacacat ggctagatcc tgaaaaagct ggagaagaag
     4681 cccaaattat cgctgataaa ctttcagagg tggatagtga gcataaagag acttatcaaa
     4741 aaaatgcgca agcctttatc aaaaaagctc aggaattgac taagaaattc cagtctaaat
     4801 ttgaaaaagc gactcagaaa acatttgtaa cacaacatac agccttttct tatctagcga
     4861 agagatttgg gcttaatcaa cttggtattg caggcatctc tcctgaacaa gaaccaagtc
     4921 cacgacaatt aacagaaatt caggaatttg ttaaaaccta taaggttaaa acgattttta
     4981 cagaaagtaa tgcttcttca aaagtagctg aaactcttgt caaatcaaca ggtgtgggtc
     5041 ttaaaactct gaatccttta gaggcagacc cacaaaatga caagacctat ttagaaaatc
     5101 tggaagaaaa tatgagtgtt ctagcagaag aattaaagtg aggaaagaat gaaaatcaat
     5161 aaaaaatatg tagcaggttc agtggcagtt cttgccctaa gtgtttgttc ctatgagctt
     5221 ggacgttacc aagctggtca ggttaagaaa gagtctaatc gagttgctta tatagatggt
     5281 gatcaggctg gtcaaaaggc agaaaatttg acaccagatg aagtcagtaa gagagagggg
     5341 atcaacgccg aacaaattgt tatcaagatt acggatcaag gttatgtgac ctctcatgga
     5401 gaccattatc attactataa tggcaaggtt ccttatgatg ccatcatcag tgaagaactt
     5461 ctcatgaaag atccgaatta tcagttgaag gattcagaca ttgtcaatga aatcaagggt
     5521 ggctatgtga ttaaggtaga cggaaaatac tatgtttacc ttaaggatgc agctcatgcg
     5581 gataatgtcc gtacaaaaga agaaatcaat cgtcaaaaac aagaacatag tcagcatcgt
     5641 gaaggaggga cttcagcaaa cgatggtgcg gtagcctttg cacgttcaca gggacgctac
     5701 accacagatg atgggtatat cttcaatgca tctgatatca ttgaggacac gggtgatgct
     5761 tatatcgttc ctcacggcga ccattaccat tacattccta agaatgagtt atcagctagc
     5821 gagttagctg ctgcagaagc ctattggaat gggaagcagg gatctcgtcc ttcttcaagt
     5881 tctagttata atgcaaatac agctcaacca agattgtcag agaaccacaa tctgactgtc
     5941 actccaactt atcatcaaaa tcaaggggaa aacatttcaa gccttttacg tgaattgtat
     6001 gctaaaccct tatcagaacg ccatgtggaa tc
//
LOCUS       DAVKAO010000088         5582 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKAO010000088 DAVKAO010000000
VERSION     DAVKAO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.088"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(495..1046)
                     /locus_tag="VWX16_001857"
     CDS             complement(495..1046)
                     /locus_tag="VWX16_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9159157.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1396..2220)
                     /gene="nadE"
                     /locus_tag="VWX16_001858"
     CDS             complement(1396..2220)
                     /gene="nadE"
                     /locus_tag="VWX16_001858"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9159158.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2217..3677)
                     /locus_tag="VWX16_001859"
     CDS             complement(2217..3677)
                     /locus_tag="VWX16_001859"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9159159.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIYNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFPEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3793..4281)
                     /locus_tag="VWX16_001860"
     CDS             complement(3793..4281)
                     /locus_tag="VWX16_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159160.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4271..4480)
                     /locus_tag="VWX16_001861"
     CDS             complement(4271..4480)
                     /locus_tag="VWX16_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9159161.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(4865..5317)
                     /locus_tag="VWX16_001862"
     CDS             complement(4865..5317)
                     /locus_tag="VWX16_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159162.1"
                     /translation="MIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIV
                     AIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMMMNTSNDLFI
                     KAIELLPFYSLPVFVASNTMSISQYLVLITTIILLYFFTLKKSKKYSF"
BASE COUNT         1717 a         1207 c          988 g         1670 t
ORIGIN      
        1 tctcatatat aaaggattgc agacatctga tctaaataca tgctaatata tttagataca
       61 aacattccaa cttgataatt ttcactcatc tttcatcatt ccttacacaa cgatgcagta
      121 taaatagaat agttttctca tcagaatgag actattttaa tattagataa tttgatagaa
      181 aaaacataaa taaaattggt tgaatttaat tccctaactc gatagaggat tctattttaa
      241 atttaacaac agctaggatt acactataaa aagtacatta ccacctgttc ggctggagaa
      301 tctagtcgat tagctccctc ttagttacaa gcttaaagcg attttttaac taatactaaa
      361 ccagattaaa gaaggaacta gaacatctgt cttgtactaa tcaatatgct attttaaaat
      421 tgcaaataaa cttggaatgc taattattat atttacaata aatagttgac ttcgatagaa
      481 acagaaaata gaattcaaca ttcttcccta cttataccat aataaataag atccgcaaga
      541 tagccttttc tctctacacc attaacaatg gtttgtagat aggacattcc agccttttcc
      601 atgacacgac ctgaagctgg gttgagactg gcataacgtg ctctgacctt ttgaaaacct
      661 gcttgagtaa aacaaaagtc caagacagct ttcaaggcct ctgtcatcat accatgtccc
      721 cagtaagcct tgcctagcac atagccgatt tcacagctta aatcagcctc gtctatctta
      781 acgatgctga tatctcctat cacttgctct gggttttctt ttagacaaat ggcccatttg
      841 taatagttga gattagtata ggaagcaacc caattgcgaa tcgagtttcg agtgatttcg
      901 acatcaggat ggggatccca ggtaacatag gtcagattct cagcggatga agcccaattt
      961 tgaaacatgg cttctgcatc actctccaca aatcttctta aaatcaaacg ttctgtctgt
     1021 aatatttgcg taccaataga tttcatgatg ctacctcact tccttatttc tatgctcgta
     1081 gtaaaaaggc tcaactcagt aacagctaga aaacgagttt gtcttctaat ttcttgacgc
     1141 agtcgctgcc tggtttgaaa tgcgatctag caagcttttt caaacctagt catcgttgca
     1201 ggggtaagac gatgaaatcg aactcttcga gttaccgaat ctgcctcatt ttcttatttc
     1261 taaactcgag gttcaagatc ccccggacct tctctcactt tcttatttca agatgagagg
     1321 ctgaaaacct ccagtggagg tttttcttgc tttttaattt cagggcaaga agctaaaaag
     1381 gtcccccgga cctttttact cccaaaagtc atcaaatacg gtgatgggta agtggcgttt
     1441 gtgttggcct ttgtgccacc agttttcaat ggtcgcttga gcttctgggc tgattgtttt
     1501 gccttctagg tagtcgtcaa tctctgcgta ggtgactcca agtgcgactt cgtcagctag
     1561 gcctggttta tcttcttcta ggtctgccgt tgggattttt tcataaaggg ctggctctgc
     1621 gccaagtttc tgcaagagct gttttccttg gcgtttattg aggcggtaaa gagggagaat
     1681 atccgcaccg ccgtcaccaa acttggtaaa gaaacctgtg atattttctg cggcgtggtc
     1741 tgttccaatg accgctccgc tatgggaacc agcaagggca tactgagcaa tcatacggca
     1801 acgtgccttg atattcccct tgttgaagtc tgaaacagga ctacctgtcg cttcaactgc
     1861 agctgtcatg gcatcagctg attccttgat attcacaacc aagctgacat ctggctggat
     1921 gaaggctagg gctttttgag catctgcttc atcagcttgc actccgtatg gcaggcggac
     1981 agcgataaat ttgtagctat cgtctcccgt ttcagctcgc agttcttcca tagctaattg
     2041 cgccaaacgt cctgccaagg ttgagtcttg tcccccagaa atccctagta caaaggtttt
     2101 taggaaggga tgttttttca gatatctttt taagaaatca atagaacgac ggatttcttc
     2161 ctgggcatca atcactggtt tgacacccag ctcttggata atcgtttctt gcaaactcat
     2221 tcttcttctc cttcaccaag ggcttccttg cgcatcttat caatcaagtc catcttatct
     2281 tgccatacgt cacgcgccaa atccactgga tagtgctgcg gattgagcac acgcttatac
     2341 tcatcccaca acttgtcaaa ttccttacgg gcataatcct gaatgtcagt caaactaggc
     2401 aagttgtaaa ctaatattcc ttctttgaag atatccacca agagaggaac ggcatcaaaa
     2461 ttacgaaccg tcttcttgat gtatgtatag gtcggatgga acatcttgat ttctgtcatg
     2521 tcgctaatat ccacaccatc ataagtgatg tagtcacctt ctgacttgcc tttttcacga
     2581 ctggtaatgc gccacacctg cttcttacct ggcgtcgaca ctttttccgc attattagac
     2641 agcttaatcg tattgcgcat ctggccgttt tcatcttcga ttgcaacaat cttgtaaacc
     2701 gccccaagag ccggctggtc ataggctgta atcagcttgg tacccacacc ccagacatca
     2761 atcttggcct tttgcatctt gaggttaagg atggtatttt catctagatc attagaagca
     2821 taaatcttag cctctggaaa tccagcctcg tccagttgct gacggacttt cttagaaatg
     2881 taggcaatat ccccagagtc aatccgcaca cccataaagt taatctgatc acccagctca
     2941 cgcgccacct gaatggcagc tggtacaccg atgcgaaggg tatcataggt atccacaaga
     3001 aagacacaat ttttgtgggt cgcagcgtaa gccttgaaag cctcatagtc attgccataa
     3061 acctgtacca aggcatgggc atgggttccc aaaacaggaa tgtcaaagag cttacccgca
     3121 cgcacgttgc tggttccatt ggcgccacca atcaccgctg cgcgtgttcc ccagatggcc
     3181 gcatccattt cttgagcccg acgtgtccca aactccatca agggttcatc ttcgataacc
     3241 gaacgaatac gagctgcctt cgtcgccacc aaggtctggt agttgacgat gttcaaaaga
     3301 gccgtttcga ccaactgaca ttgggctaga ggtccttcca cctgcacaat cggttcatta
     3361 gcaaaaacca aatccccttc ttgggcagaa cgaacggtca actccaactt gaaattgcga
     3421 aggtaatcca agaacgcccc atgataacca agcgactcca aataggctat atcactatct
     3481 gaaaaacgca agtcttcaag atagttcaca attctttcca aacctgcaaa aaccgcatag
     3541 ccgttcttaa aaggctgttg gcggaaatac acctcaaaga ccgccttctt attgtaaatc
     3601 ccttgatcaa agtaaacctg catcatgttg atctggtaca agtccgtgtg caatgtcaaa
     3661 ctatcatctg gatacatact tttcctactt ccttagctag aaacccatga aaattttcaa
     3721 gaactttcat gtattccaat aaattagtac tattatatca cattttagct ggattgagaa
     3781 aagagtaaca agctattctc cactctccaa ttcatccata tcttgttcaa attttttctg
     3841 agcccattcg ccatagctct taagaccaag attgccaata aagacccacg gaaggtaaat
     3901 gacataagta atgacccaag cagacaggta tttaaaattc aaaggattgt gctgataaat
     3961 ttctatgttg aattgataat tctgcaacat caaaagagcc gtaatagcca aggttaggaa
     4021 aaaacaaccc aaaatcgtaa aatgaaaacg actatagtag gtcactccca gataacgggc
     4081 acgattgaaa aagtaaaatg tccctatgat gataacgatt agcagcatat tagaattaaa
     4141 aaggcttggt gctaatactg aaatgatata agataggagc gacaaagcaa tgcagatata
     4201 gaaactttca gagcccgctt tattgaacag ttgttcttct ctttcgtcta gtaattgata
     4261 ataataaaat ctatttttca tcttcttcct cccaaaatag ttggtctagg gttttcccta
     4321 aacatctgca aatagactgg cagagcgaga gactgggatt gtattttccc gcctctatca
     4381 aaccaatagt ctggcgtgtc accccgacag cctctgccag ttgaccttgt gttaaatcac
     4441 gctctacccg agctaatttt aattttaaat ttttagccac cttcgtcctc cttatagttt
     4501 taatactcat ctactcttaa aaaatccaaa accaacacaa gctatcagct atttattata
     4561 accccattaa tttgcactcc agtattatct tcagccgaaa atcagtattt taggttgttt
     4621 gaaataaact tcctagttta ctcttttatt tttatttagt attagcttta gtaggatctt
     4681 ttaaacgagt ttttattgct tatatttctt gtttagttta gttggtttca tatcactatt
     4741 atataatgct tttggaaatg agcctagtat aagtcaaaaa cgtgttgaat agtgaaagca
     4801 ggagcatacc aagatgactg attttttact gtattttagg tatgcaaaat aatactaaat
     4861 gaaactaaaa tgaatacttc ttgcttttct tgagagtgaa aaagtacaat aaaatgattg
     4921 tagtgattag cacaagatac tgactaatag acatcgtatt tgaagccaca aatacaggta
     4981 aagaataaaa aggaagaagt tcaattgctt tgataaacaa atcattacta gtattcatca
     5041 tcatcgtatt taatatatta gggataaaga gtagtaaagc tactaaaata ccaaatacca
     5101 agccactttt caatttgtat aaatagaaaa tgtaagataa tagaaaataa aacaggacta
     5161 acaaaattgt attgactaca atagctacta taaaatctaa tcctaaatca cctggaatac
     5221 ccaatttact tactatgact agtaacacaa tgccattcaa agcataaata atggaaactg
     5281 agatggcata tagaaaatta gacaagacgt aaatcatccg attcttctgt tgattataaa
     5341 acaaacttac cgtattgtgg gaaaaatctc tactaatgac tcaattagca tgtataacag
     5401 ccattaacat accgagcata gcataaaagt ttgaaatatg cttaatacca agcgatgtcc
     5461 cttgagtctt tagaataaag aatgtaacca atatcggcgg tataaatgag ataagcaaac
     5521 cgagatatat tacacctgat gaatataaat ttcttatatt ttccttaaga aaattaaatt
     5581 ta
//
LOCUS       DAVKAO010000089         5574 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKAO010000089 DAVKAO010000000
VERSION     DAVKAO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.089"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            226..>5574
                     /locus_tag="VWX16_001863"
     CDS             226..>5574
                     /locus_tag="VWX16_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9159163.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSNSEDALSISDKEKV
                     AENKEKHENIHSAMETSQDFKEKKTAVIKEKEVVSKNPVIDNNTSNEEAKIKEENSNQ
                     SQGDYTDSFVNKNTENPKKQDKVVYIAEFKDKESGEKAIKQLSSLKNTKVLYTYDRIF
                     NGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGQSFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLEKPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAVTTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALSSNGKKTDFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAGSTSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVSREHF
                     IRGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIEGKSLF
                     NIHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIAYDDGT
                     DLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEIKATK
                     YDFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKETKSEY
                     PYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKV
                     TTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRSTQSVLM
                     SALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKFNVEA
                     IKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRV"
BASE COUNT         2189 a          800 c         1069 g         1516 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggagt ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctaactc tgaagatgct ttaagcatct ctgataaaga aaaagtagca
      361 gaaaataaag agaaacatga aaatatccat agtgctatgg aaacttcaca ggattttaaa
      421 gagaagaaaa cagcagtcat taaggaaaaa gaagttgtta gtaaaaatcc tgtgatagac
      481 aataacacta gcaatgaaga agcaaaaatc aaagaagaaa attccaatca atcccaagga
      541 gattatacgg actcatttgt gaataaaaac acagaaaatc ccaaaaaaca agataaagtt
      601 gtctatattg ctgaatttaa agataaagaa tctggagaaa aagcaatcaa gcaactatcc
      661 agtcttaaga atacaaaagt tttatatact tatgatagaa tttttaacgg tagtgccata
      721 gaaacaactc cagataactt ggacaaaatt aaacaaatag aaggtatttc atcggttgaa
      781 agggcacaaa aagtccaacc catgatgaat catgccagaa aggaaattgg agttgaggaa
      841 gctattgatt acctaaagtc tatcaatgct ccgtttggga aaaattttga tggtagaggt
      901 atggtcattt caaatatcga tactggaaca gattatagac ataaggctat gagaatcgat
      961 gatgatgcca aagcctcaat gagatttaaa aaagaagact taaaaggaac tgataaaaat
     1021 tattggttga gtgataaaat ccctcatgcg ttcaattatt ataatggtgg caaaatcact
     1081 gtagaaaaat atgatgatgg aagagattat tttgacccac atgggatgca tattgcaggg
     1141 attcttgctg gaaatgatac tgaacaagat atcaaaaact ttaacggcat agatggaatt
     1201 gcacctaatg cacaaatttt ctcttacaaa atgtattctg acgcaggatc tgggtttgcg
     1261 ggtgatgaaa caatgtttca tgctattgaa gattctatca aacacaacgt tgatgttgtt
     1321 tcggtatcat ctggttttac aggaacaggt cttgtaggtg agaaatattg gcaagctatt
     1381 cgggcattaa gaaaagcagg cattccaatg gttgtcgcta cgggtaacta tgcgacttct
     1441 gcttcaagtt cttcatggga tttagtagca aataatcatc tgaaaatgac cgacactgga
     1501 aatgtaacac gaactgcagc acatgaagat gcgatagcgg tcgcttctgc taaaaatcaa
     1561 acagttgagt ttgataaggt taacattgga ggacaaagtt ttaaatacag aaatataggg
     1621 gcctttttcg ataagaataa aatcacaaca aatgaagatg gaacaaaagc tcctagtaaa
     1681 ttaaaatttg tatatatagg caaggggcaa gaccaagatt tgataggttt ggatcttagg
     1741 ggcaaaattg cagtaatgga tagaatttat acaaaggatt taaaaaatgc ttttaaaaaa
     1801 gctatggata agggtgcacg cgccattatg gttgtaaata ctgtaaatta ctacaataga
     1861 gataattgga cagagcttcc agctatggga tatgaagcgg atgaaggtac taaaagtcaa
     1921 gtgttttcaa tttcaggaga tgatggtgta aagctatgga acatgattaa tcctgataaa
     1981 aaaactgaag tcaaaagaaa taataaagaa gattttaaag ataaattgga gcaatactat
     2041 ccaattgata tggaaagttt taattccaac aaaccgaatg taggtgacga aaaagagatt
     2101 gactttaagt ttgcacctga cacagacaaa gaactctata aagaagatat catcgttcca
     2161 gcaggatcca catcttgggg gccaagaata gatttacttt taaaacccga tgtttcagca
     2221 cctggtaaaa atattaaatc cacgcttaat gttattaatg gcaaatcaac ttatggctat
     2281 atgtccggaa ctagtatggc gactccaatc gtagcagctt ctactgtttt gattcgacca
     2341 aaattgaagg aaatgcttga aaaacccgta ttaaaaaatc ttaaaggtga tgataaaata
     2401 gaccttacaa gtcttacaaa aatagcccta caaaatactg caagacctat gatggatgca
     2461 acttcttgga aagaaaaaag tcaatacttt gcatcaccta gacagcaggg ggcggggcta
     2521 attaatgtgg ccaacgcttt gagaaatgaa gttgtagcga ctttcaaaaa cacggattct
     2581 aaaggtttgg taaattcata tggttccatt tctcttaaag aaataaaagg tgataaaaaa
     2641 tactttacaa ttaagcttca caatacatcc aacaggcctt taacctttaa agtttcagca
     2701 tcagcggtaa ctacagattc tctaactgac agactaaaac tggatgaaac atataaagat
     2761 gaaaaatctc cagatggtaa gcaaattgtt ccagaaattc atccagaaaa agtcaaaggg
     2821 gcaaatatta catttgagca tgacactttc actatagggg caaattctag ctttgattta
     2881 aatgcggtta taaatgttgg ggaggctaaa aataaaaata aatttgtaga atcatttatt
     2941 cattttgagt cagtagaaga aatggaagca ttaagctcca atggcaagaa aaccgacttc
     3001 caaccatctt tatcaatgcc cctgatggga tttgctggga attggaacca cgaaccaatc
     3061 cttgataaat gggcttggga agaaggatca aaatcaaaaa caatggaagg ttatgatgat
     3121 gacggtaaac caaaaattcc aggaacctta aataagggga ttggtggaga acatggtata
     3181 gataaattta atccagcagg agttatccaa aatagaaaag ataaaaatag aacatctcta
     3241 gatcaagatc cagacttatt tgcctttaac aaccaaggtg ttcatgcagg atccactagt
     3301 ggttctaaga ttgctaacat ttatccttta gattcaaatg gaaatcctca agatgctcaa
     3361 ctagaaagag gactaactcc atctccactt gttctaagaa gtgcagaaga aggactaatt
     3421 tcaattgtaa acaccaacaa agaaggagaa aaccaaaaag accttaaagt tgtatcaaga
     3481 gagcacttca taagaggaat tctaaattct aagggaaatg acgctaaggg tatcaaatca
     3541 tccaaactaa aagtttgggg tgacttgaaa tgggatggac tcatctataa cccaagaggt
     3601 agagaagaaa atgccccaga aagtaaggat aaccaagatc cagctactaa gatacgagga
     3661 caatttgaac cgattgcgga aggtcaatat ttctataaat ttaaatatag attaactaaa
     3721 gattacccat ggcaggtttc ctatattcct gtaaaaattg ataacacagc ccctaagatt
     3781 gtttcggttg atttttcaaa tcctgaaaaa attaagctaa tcacaaagga tacttatcac
     3841 aaggtaaaag atcaatacaa gaatgaaact ttatttgcta gagatcaaaa agaacatcct
     3901 gaaaaatttg atgagattgc aaatgaggtg tggtatgcag gagctgctct tgttaatgaa
     3961 gatggagagg ttgagaaaaa tcttgaagta acttatgcag gtgagggtca aggaagaaat
     4021 aggaagcttg ataaagatgg gaatactatc tatgaaatta gcggagcagg agatctaaga
     4081 ggaaaaatca tcgaagtaat cgccctagat ggagcaagca actttaccaa aatccatagg
     4141 attaaatttg ctaatcaagc tgatgaaaaa gggatgattt cctattatct agtagatcct
     4201 gatcaggatt catctaaata ccaaaagctt ggcgaaattc ctgaatctaa atttaagaat
     4261 ttaaaaaatg ttaaagatga tagcctaaac aaagagacag ctgaggtgga aaataacctt
     4321 ttagtagata atcagtctat cgaaggaaaa tctctcttta atattcataa aactatttca
     4381 acaattagag actttgaaaa taaagaccta aagaaactca ttaaaaagaa atataagcaa
     4441 gaagacgact ttgtaaatgg tggaacaaga acagtagagc gtgattataa atacgatgat
     4501 aaaggaaata taatagccta cgatgatggg actgatctag aatatgaaac tgagaaactt
     4561 gacgaaatca aatcaaaaat ttatggtgtt ctaagtccgt ctaaagatgg acactttgaa
     4621 attcttggaa agataagtaa tgtttctaaa aatgccaagg tatattatgg gaataactat
     4681 aaatctatag aaatcaaagc gaccaagtat gatttccact caaaaacgat gacatttgat
     4741 ctatacgcta atattaatga tattgtggat ggattagctt ttgcaggaga tatgagatta
     4801 tttgttaaag ataatgatca gaaaaaagct gaaattaaaa ttagaatgcc tgaaaaaatt
     4861 aaggaaacta aatcagaata tccctatgta tcaagttatg ggaatgtcat agaattaggg
     4921 gaaggagatc tttcaaaaaa caaaccagac aatttaacta aaatggaatc tggtaaaatc
     4981 tattctgatt cagaaaaaca acaatatctg ttaaaggata atatcattct aagaaaaggc
     5041 tatgcactaa aagtgactac ctataatcct ggaaaaacgg atatgttaga aggaaatgga
     5101 gtctatagca aggaagatat agcaaaaata caaaaggcca atcctaatct aagagccctt
     5161 tcagaaacaa caatttatgc tgatagtaga aatgttgaag atggaagaag tacccaatct
     5221 gtattaatgt cggctttgga cggctttaac attataaggt atcaagtgtt tacatttaaa
     5281 atgaacgata aaggggaagc tatcgataaa gacggaaatc ttgtgacaga ttcttctaaa
     5341 cttgtattat ttggtaagga tgataaagaa tacactggag aggataagtt caatgtagaa
     5401 gctataaaag aagatggctc catgttattt attgatacca aaccagtaaa cctttcaatg
     5461 gataagaact actttaatcc atctaaatct aataaaattt atgtacgaaa tccagaattt
     5521 tatttaagag gtaagatttc tgataagggt ggttttaact gggaattgag agtt
//
LOCUS       DAVKAO010000090         5409 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKAO010000090 DAVKAO010000000
VERSION     DAVKAO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.090"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..195)
                     /locus_tag="VWX16_001864"
     CDS             complement(<1..195)
                     /locus_tag="VWX16_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159164.1"
                     /translation="MLIVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLK"
     gene            complement(203..574)
                     /locus_tag="VWX16_001865"
     CDS             complement(203..574)
                     /locus_tag="VWX16_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU9159165.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            complement(719..2071)
                     /locus_tag="VWX16_001866"
     CDS             complement(719..2071)
                     /locus_tag="VWX16_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU9159166.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQTLQNELIQLQQQGDSSSLSDTTSDDDKAK
                     LETKISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLISEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILASNEGTLHYLVPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKVDVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVILQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(2068..2535)
                     /locus_tag="VWX16_001867"
     CDS             complement(2068..2535)
                     /locus_tag="VWX16_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9159167.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(2528..4747)
                     /locus_tag="VWX16_001868"
     CDS             complement(2528..4747)
                     /locus_tag="VWX16_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU9159168.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDITLSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIMDKTTIVVAHRLSTILR
                     CDKICFLENGTIVEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
     gene            5213..>5409
                     /locus_tag="VWX16_001869"
     CDS             5213..>5409
                     /locus_tag="VWX16_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9159169.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYI"
BASE COUNT         1752 a         1069 c          745 g         1843 t
ORIGIN      
        1 ttttaaaaaa cttaataata aatttgctcc actgattgct atcaatattt caattggtaa
       61 aaaatagtaa ttccaaataa aatgagaaat cagcaatgat agaaaaataa caatatcaat
      121 tttcctgttg ttgacatact ctgtaatttt atgatacagg aatgcaacaa taactgaaat
      181 taaaacaatc aacataagat tattaaccta ttctcaattg attatcacgt cgcccatccc
      241 agccatcaat aatattggta aaaatccaat cagctccagc accaatccca gcagatgcaa
      301 tacttctaat aacagtattt gcaaaagcat ttccaatatg aattcctaat actcctaaac
      361 ctttaacaat cccagctacc agtgcggagc gtaatccata agatgcaaga actagttgaa
      421 ggcctccacc tcctgctgct gctaatgctg catatgctgc gccagtaatg gctcctgtaa
      481 ttgcccacct gggtatatat atagcccccc catcaaggta catcatctct tcttcatcaa
      541 tcacaacata attatttggt aatacaagtt ccatttgttt tctccttttt attaagcatg
      601 gttaggatgg tttcctaaca agtaatcgtc gactgtgttt ctatcactta gctacctaga
      661 taaaatatcc tttctctacc ttatttgttt ttataggtta cctaaggttt aaaaccaatt
      721 attgcttgaa acttaacatt tctaaaatcc aatcaagata ggtttcttta tcatagacaa
      781 tccgcacttc aactggcata tccttttgga gtatgaccgt ctcgcttcca tgttttagag
      841 ttaaggtttc taattctatc atgactttat agaggctaat attcccctct ttcgtttctt
      901 gggaaattgt tcctgaatca atctgtctga cttgtccctt tagtgttcca tatttttgac
      961 tattcacacc agtaatagca acatcaactt ttgctccttt tgaaacacga gaaatatcac
     1021 tcgcaagtac aaaagcctct acatagtaac ctttttcttt ccctgaaact tctgctatcg
     1081 tttgcccctg ctgaatagac attccttgtt tcaaaggtac cagataatgc agagtacctt
     1141 catttgacgc taatatacta tgagctttat cttgacctgt tgccactcca agattagcct
     1201 caagctctgt aatactttta ttgttgtttg aacgagcagt acctaactca ctaattaatt
     1261 gagcataaat attataagcc tgagaagttg gatcattata ggtacggttc atatccttaa
     1321 tattgctttg ttgactatca atctcgctat tcttagaagt aatatttgtt tttaatgctt
     1381 cgatttttgt tgttatttct gatatcttag tttctaactt agctttatca tcatctgatg
     1441 tcgtatctga taaggaggat gagtctcctt gctgttgaag ttgaatcaat tcattttgta
     1501 gagtttgcaa gtcggcatct aactgatttc tttcagctgt tattttattc aacttcgtat
     1561 attcatcctg aatctttgaa tactgggtac cattattata attttccgag tttaactgag
     1621 acagatagta ttcaactttc ccataatatt cctgttcttc accagaatta gataaatgat
     1681 tgtatttctc atttaatgat tgttcaaatt tttgaaagat agcttctttt gcacgtaact
     1741 tctccaattg tttatttaaa gagctcgctt gtaaaccttc ttgtccactt gataacgaaa
     1801 taatgacatc tccagcattt accatatctc cttctgactt atgtaaagta actaccttcc
     1861 ctgaaccaat tgctgatagg aactctgtac ctgttataac tgaatttcca ttcgctttta
     1921 caatatagtt tttgggtata taagctgcgc caaccaatgc accgcttaag ataatagcag
     1981 ttgaaataat gagaataaac gcaaaagctg gtggtctctt atcaaagaaa atacgagaat
     2041 aacgtaattc tgatttatta tataatttca taggcttaca attggtctaa aaatatctac
     2101 taccattttt tcaggagaag aattaacata aactgtatag acaatcccat ccgtttgaat
     2161 atcattttca tagacatata gatccaattt agaatacgca tactgtagat actctggact
     2221 gtcttcaaaa cgaacatata aacaatatgg aacagagata gaatcctgta catcataaat
     2281 gttactgtac tgttgagcat tatgagcttg aatataaaac tcaaaatcag tcgttattaa
     2341 tccatcatca tgaatagtag taccacaact ttttacaatt aatggaccaa aaatttgtgc
     2401 ttttaacaac tgcaaatgtt gatgaaattt attaatttcc tgatctacat cttctacttt
     2461 tgtatcatgt aactttttac agataactga ctttagtacc agttttttat tatcttttac
     2521 ctctaactta gccataagta acctcctctg tatctaacac agcctgtgac tgaatttgtt
     2581 gattcacttg aacgctctgc aaaccaacca ttctagcata ctttccattt ttcgccatta
     2641 gttcttcatg agtcccatat tccacaatag tcccattttc taaaaagcat atcttatcac
     2701 atcgaagaat tgtagacagc ctgtgggcta ctacaattgt tgtcttatcc attattttat
     2761 taaagattaa atcctgaata atctgttcac taaatgaatc taagttagag gttgcctcat
     2821 caaatatata caaatcagct ttactcagta gtgctcttgc aatagccaat cgttgtttct
     2881 ctccacccga gaaattttga ccgccttctt ctacaaagga gccataacga ttttgaagac
     2941 gttgaatcgt atcatgaata ccaacaatac gacaaactct caccatatcc tcatatgtaa
     3001 cagaaggatt accaattttt agattatcaa taattgtccc tgtaaagagt tctatggtct
     3061 gaggaatgta ggaaattttc ttacgtagtt taccatagtc aagatctgat aagtcatttt
     3121 ctcctatacg aattttcccc tcactcggat ttataaatct taataacaaa cgaactagcg
     3181 tactttttcc tgctccgctt tctccaacaa aagctatttt ttctccttgt ttaattcgca
     3241 aattaaagtc cttgataaca ggagggcgtg aaccataggc aaatgttaca tctttaaatt
     3301 caatgtcatc acataaggtg aagtctctaa tactataatc caaggcaatg tcttcacgat
     3361 ctacttccat caattcttga agccgactaa ccgcaacctg tacctcttga aaggttaact
     3421 gcaagccaac taaattctga ataggttctg taaaatattg acttaatgtc tgaaaaacca
     3481 agaggtcccc aatagtcatt tttccatcaa tgataaataa ggcgcccact cccataaata
     3541 ataacccacc catggttgag gtcattgaag atattgtaga ttgaatattc tgcaaaacac
     3601 cctctttata accaatttct aaagtatgaa caaatttttc ttcaattttg tttaaacgtt
     3661 gttcctcatc atgctgagac ttaatggtat caatgttacg aatagattca attaactgtg
     3721 aattaagaag tccatttgct tccatttgtt catgattaat ttttttataa ggctttttaa
     3781 aacagtatat aagaatgatg ttaacaataa ccataaaaac taatatcaga aataaagatt
     3841 gattaatagt ccaaaggaca accgcactaa taactgacaa ggtgatatcc atgactaatg
     3901 aaatagagac tgacgtaaac acattcttta tcgtcatcgc atcttgaaat cttgttaaaa
     3961 catcaccaac tctacgactt ccaaaaaaac tataaggaag atgaataata tggtcataat
     4021 atcccattaa aaccggtata tcaatttttc gagatagaaa caaaagaacg tgctgacgaa
     4081 atgcagatag aagagtctgt aagaacgaca ctattccaaa tactatcaaa aacatataca
     4141 gactattttt taaagcatat ggaatcacct cgtccatgat aacttttgaa aagagactcg
     4201 ataaaatacc aatgatactt aaaacaaagg aagctaatat gactgtacta atcaatcctt
     4261 tttgattaaa aattaacctt ccaaataaat caagaagtcc ttttcccttt agtttccctc
     4321 tttcaaaatc cgaatttgga accataaaaa gagtaatacc ttgataaatt tctcccagtt
     4381 cgtcacttga catcttgcgt atgccactag cagggtctgc tacgtagaat ttactattcc
     4441 ttttgatact atgtaatacc acaaaatgat tttgacctaa atcattctta acttgaagaa
     4501 tcgcaggaaa tgttaatttt ggagtcaaat cttccagtgc tactcgaaca gcttttactg
     4561 taaaattcaa cttattcaga cctgaaacaa taccactgac agtcgttcca tacatgtctg
     4621 taccaataat ttcccgaatc ttcatgattg atagttcctt tttgtaagat aaaagaactg
     4681 ttgaaacaac tgccgcagca caatctgact ggtcatgttg catcgtacaa ggatattttt
     4741 ttaacataga aatactcaat tctttagaat tcatctaact tttctttgat gtcacccaaa
     4801 cctcaatcac tgcccagtag ttcttgaatt agtttatttt caaccgtatc attctttgtt
     4861 cttgatatct ttccaagcat tgtagtacag cagatttttg tccaaggact gagcctcctt
     4921 taacaactca agctccctta cattatatcg gccagcccca tggaaagatt atagccagac
     4981 taatcaatga tataatacat actagaatac aaaatatttt tttattcata ctttttctcc
     5041 tatctttatt ttctacctac atcatacctc ttattaagct catttcaaaa ttacgttaac
     5101 ttttttcaca aatttatttt tactttcttt tttacttttt acactgctat gatacaatat
     5161 ttttaacaag attttattat ttgttaactt ttttcacaga aaggagaaaa tcatgcgtta
     5221 tgatttcgga aaggtctata aagaaatacg tgagtcaaag gggctgaccc aagaagaggt
     5281 ctgtgggggt gttctctcaa gaaccagctt atcaaaaatc gaaagtggca agacaacccc
     5341 aaaatatgaa aatatggaat ttcttctccg tcaaatcaat atgagttttg aagagtttga
     5401 atatatctg
//
LOCUS       DAVKAO010000091         5093 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKAO010000091 DAVKAO010000000
VERSION     DAVKAO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.091"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..502
                     /locus_tag="VWX16_001870"
     CDS             <1..502
                     /locus_tag="VWX16_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159170.1"
                     /translation="RIRLKSIKHFTSFSFKLAALSTGIWTATLFLLIFLIAFSNGFSF
                     SLEIKEVDFLREFYGISIANNASFFIGFFFSYIAYYFFLSLLTISSFSWFKKSNMSLV
                     FLFTFLFVESLFWIYQLDNGIIGLLPIFQYMVNSNPYALIYWLTLLSIIIPLTVFSVH
                     RNWRRV"
     gene            512..1210
                     /locus_tag="VWX16_001871"
     CDS             512..1210
                     /locus_tag="VWX16_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159171.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAIVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANEQMNFSCVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            1207..1854
                     /locus_tag="VWX16_001872"
     CDS             1207..1854
                     /locus_tag="VWX16_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9159172.1"
                     /translation="MNEIITLKNIQLELKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(1941..2807)
                     /locus_tag="VWX16_001873"
     CDS             complement(1941..2807)
                     /locus_tag="VWX16_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9159173.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGPSSGKTTAKARTID
                     ENKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIKKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGKVD"
     gene            complement(2819..3576)
                     /locus_tag="VWX16_001874"
                     /pseudo
     CDS             complement(2819..3576)
                     /locus_tag="VWX16_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(3577..4254)
                     /locus_tag="VWX16_001875"
     CDS             complement(3577..4254)
                     /locus_tag="VWX16_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9159174.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(4214..4894)
                     /locus_tag="VWX16_001876"
     CDS             complement(4214..4894)
                     /locus_tag="VWX16_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9159175.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIHLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFG
                     NPSTLSGK"
BASE COUNT         1524 a          845 c         1071 g         1653 t
ORIGIN      
        1 tcgtattcgt ctgaaatcaa ttaagcactt taccagtttt agtttcaaat tagcagctct
       61 tagtacgggg atttggacgg cgactttatt tttattgatt tttctgattg catttagtaa
      121 tggttttagc ttctctttgg agataaagga ggttgatttt ttaagagaat tttatggtat
      181 aagtattgca aacaatgcta gtttctttat aggatttttt ttctcttata tagcatacta
      241 tttcttttta tccttactta ctattagcag tttttcttgg tttaaaaaat caaacatgag
      301 cttagtattt ctgtttactt ttttatttgt agaatcctta ttctggattt atcagttgga
      361 caatgggata attggattat tgccaatttt tcagtatatg gtaaattcca atccgtatgc
      421 attgatttat tggcttacat tactatctat cataattcca ttgactgtat tttctgttca
      481 tagaaactgg aggagagtgt aaaagttgga aatgggaaag ttaagtagtc acatgtggag
      541 gttgaatcag ataatctata ccaagtactt ttggggttat gttctttttt ggatattgat
      601 ttgtttagga ttatggtatt ggttagaagg aaatgataga cttgttatag aaattttaaa
      661 agggcctaat ctgagtcaaa actctttttt agtcttatct atatggttgc ttcattggtt
      721 tattattcat acattttttc tagcaattgt atatcgtaga agagcatccg atttctttat
      781 ggaagtgatt cgattttctt ctattaagct ctggattagg tatcagattt ggacctgttt
      841 tctttatgga ctcattttaa tcatggtaaa agttctagtg attcaattta tgttacagtt
      901 accaaactgg gatataggag ttttgtttat agttgattct ttgaatgctt gtgtgttagt
      961 cttgttttgc tttatgttat acgcactagg agcgaatgag caaatgaact tttcttgtgt
     1021 tagcttcttt ttactcatga ttgtgtttgg tggtctattt gtaggaaatc gaactaatta
     1081 tctattttat attttaaatc gagggaatgg agatatagga cgagatttat ttcttcagtt
     1141 gctattttta gtctttcttt ttaaaagcat tttctatttc actcgtcaaa agaggaggtt
     1201 tatagaatga atgaaataat tacattaaaa aatattcagt tggaattaaa aaaaacatgt
     1261 gtttttcaaa accttaactt tagttgtaaa cagggggaaa ttataggaat tactggtgcg
     1321 aatggctcag ggaaaagtgt attgtttaaa ttaatagctg gtttatatag tccgtcttat
     1381 ggagaagtgt taatcaatgg ggaaaatatt gttcctgaga gaaaaattcc agctaatttg
     1441 ggagctttga ttgaagaacc tggttttata aattattata gtggctttaa gaatttacaa
     1501 tatttggcaa gcatacgagg agtagttggt aatcaggaaa tcaatgatac actgaaaata
     1561 gttggtctat atgagcaaaa agaccagaaa gttaaaactt attcgctagg tatgaggaaa
     1621 aagctaggga ttgctcaagc aattatggag aatccctcta ttcttttact agatgaacct
     1681 atgaatgcct tggataaatc aagtgtagaa aatatgagaa cattgtttag aaagctctct
     1741 agtgaaaaag ggacaacaat tttgattgct agtcatagtg aagaggatat tcgtatctta
     1801 tgtgataaag tatatgcaat agaagataaa gtatgtacac tgtgttcaga ttgaacatct
     1861 tagatagact caggatgtta taggtgaaat cttaaaaagg gattggatct taaaatccag
     1921 ttccttttaa gagttaatga ctaatcaact tttccacctt caacaaccag gtcatctgct
     1981 ttagcagcgt caccgtaggt tgggtgaagt gttttttcat aggccttgtg gaagaagttt
     2041 tccttgccta attttttaat atctttattg atgaagtcta gcaattcttg gttgcctttt
     2101 tgaactgctg ccgcaatggt atcgggatca ccgagggaag taattcctac ttcaaatcct
     2161 ttattttcaa gcgcccaagc tagaacttcc gtattgtcag ttgaaaaggc atctccacgt
     2221 ccgtcaagaa gagcttggta agagtcactg tattggtcgt atttttggag tttgatttct
     2281 ggatgattct tttcaaaata agtctcagca gtcgttcctt ttgtgacaat taaggtttta
     2341 ccttcaagtt gtttgacgtc tgtaatgaga ccagtcttag gtgatacgac acccagagaa
     2401 actttcatat atggaagggc aaaatcaact tgtttcttac gttcgtcagt tactgtaaag
     2461 ttagcaagag taatatctac cttgtttgaa atcaagtatt ccgcacggtt ggcagcatcg
     2521 actgaaatgt atttaacctt gacaccaagg tcttgagcta gttggttccc tagttcaata
     2581 tcgtagcctt ggtaagaacc atcattgtca acgtagccaa acggtttttt atctccaaac
     2641 acggcgattc gcagttcacc gctttttttg ttttcatcga tagtgcgagc tttggcagtt
     2701 gtttttccag aggatggacc agcgtttcca cctgagctac aagcagtgat aaagataagg
     2761 gcaaaggcaa gtgctaaaac agttaagagt ggtttgaata gtttcataag aatctccttt
     2821 atagatatga gccgaattgg ctaaagtcaa agacgtttaa aaattcctgg gctcgtttgg
     2881 tttgcggatt ggtaaagaag gcttgagctg ttccttcttc agcgattttc ccttggtcga
     2941 ggaagataat ccggtcagca atggcttggg caaactgcat ttcgtgggtt actaaaatca
     3001 tggtacggcc ttcttggacc aaatcattga taagttccag cacctcacgc accatttctg
     3061 gatccagcga agcagtcacc tcgtcaaaaa ggatgatttc tggatgcatt aggagggcac
     3121 ggacaattgc aacacgttgc ttctgtccac cagataattg acgggcaaag ctatgttgtt
     3181 tatccagcaa accgacacga tctagtaatt gcaaagcttc ttccgttact tctttcttgt
     3241 cccttccttg agttttgata gggcctagga tgaggttttg taagacatcc agatggggaa
     3301 agagttcata actttgaaag accatgccaa tcttttggcg aactaggtga aaatcttttt
     3361 tattttcaac gatagactga ccatccagaa aaatatctcc accttgaata ctttctaagc
     3421 cgttgaggca acgaaggagg gtacttttcc cacaaccaga gggccctagg atgacaacaa
     3481 cttccctttt ttgatttcta gagaaagtcc ttggaggatg ggattgtctc cgaaggattt
     3541 ttttagttcc ttgatttcta agatagtttc agacatttag tttctccaat gtttttctaa
     3601 gtgagtggat agtttggaaa tagggtagca aactgcgaaa tataagacta gaatggttcc
     3661 ataaatccaa aatgaagcag ttgggatggt caggcgattg ctatcgatga tttgttgtcc
     3721 aactttggtc acttccacaa ccccaatcaa aacaactaat gaagtggttt taatcatccg
     3781 agtgacaaga ttgatagcct gcggtagcag tcttcttaag acttgtggga tgatgatgtg
     3841 gtagtaaagt tgaacattag tcaagccgag tgcctgtcca ctttcaaact gatgtttagg
     3901 gagagaagtg atagctccac gtaccaagtc tcccatttca gctgttcccc agagggtaaa
     3961 aacgataata gctgaagtct cacctgagat attgatatta aagtttcgag ctaagccaaa
     4021 gtaaacgatg aagagtagca ccagctgggg catgatacgg gtaaagtcca gatacaatcg
     4081 tgttaaaaat cgtatgattc tagaatggga ggtcatgatg attcccatga ctgttccgaa
     4141 catcatggat aagaggacag acaggatgga tatctcaatc gtaacgccca atccctgtaa
     4201 gattctcagg agattatttc cctgaaagag tacttggatt cccgaatcct gcatggcgga
     4261 gcctcctttc tatccagcta aagaccagtg agatgggtag cagcatgatc agataagcaa
     4321 ctaccaacat agctagcgca atgtctgtct catagtagag accaatcaaa tccttggcga
     4381 catacatgag gtcggccaaa gccactgctg agaaaacaga ggtttccttg ataaggaaaa
     4441 tgacattggc actaaaggag gggagtgcca ccgctgttgc ttgcggaaga accacatagc
     4501 gaaagacctg tagaggtgtc agaccaatag cgaggccaat ctcctgctgg gtttgactga
     4561 tggcttccag cccacttcgg aaagattctg ccatatagga tcctcctaaa aagacaagcc
     4621 caagcgttgc acagacttct gaagatagga caatcccgat tcggggaaga ccgaagtaga
     4681 gaaagaagag atgaatcaaa aggggcgtat tacgtgacaa ttcaatgtag gctgtcgcta
     4741 cttgcgccaa aacagggatt cgataatgtc ggatgatact aacgattaaa ccgagcagaa
     4801 aggatcccaa aattccccaa actgcaatat gcaaggtcag aaagaatgcc ttttgatata
     4861 gtggtagata ttgttcaaca atggaccaat ccaaaaatag aacctcccat ctagaaataa
     4921 tacagttatt gtagcactta aaatcttctt tggataatat ctatttttta ttgccgttat
     4981 aaggattttt taccatagac ataaaatttc tgaaatttcc aaacaaaata tttgaaaagt
     5041 tttgaaaaag agttaagata tttttgtaat atacaaagtg aacgcttact tat
//
LOCUS       DAVKAO010000092         4921 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKAO010000092 DAVKAO010000000
VERSION     DAVKAO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.092"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..217
                     /locus_tag="VWX16_001877"
     CDS             <1..217
                     /locus_tag="VWX16_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9159176.1"
                     /translation="KLFAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQ
                     TEYNYLLTSSELVYLMAHIRRLTKNLD"
     gene            235..2073
                     /locus_tag="VWX16_001878"
     CDS             235..2073
                     /locus_tag="VWX16_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9159177.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDVPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLVASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPAVYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLILPNNQTIEHSKELLVIL"
     gene            2086..3501
                     /locus_tag="VWX16_001879"
     CDS             2086..3501
                     /locus_tag="VWX16_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9159178.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAKLLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            3800..4600
                     /locus_tag="VWX16_001880"
                     /pseudo
     CDS             3800..4600
                     /locus_tag="VWX16_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
BASE COUNT         1585 a          748 c          986 g         1602 t
ORIGIN      
        1 aaaattattt gcccatcgct tggttgaaaa tacaacttat tgtgacgatg atgatgaaga
       61 cttgttagca ttaatgaaaa ataaatatcc tagagaatat gaatgtggtg aacaggtggc
      121 aatgtttata caaactgagt acaattattt gctgacttct agtgaattag tttatttgat
      181 ggctcatatt cgtcgcttaa caaaaaattt agattaatag aataggagaa taccatgagt
      241 tataaagata cggtacaaaa aatcctcgat gtaattggag gtgaaaaaaa tgtcaataga
      301 gttacccatt gtgtaacacg tttaagatta gaattaaaag atgaaaattt agtcaatgat
      361 gatgatgtga agaagatacc aggtgtaata ggtattatga aaaagaatgg acaatatcaa
      421 attatacttg gtaatgatgt agctaattat tataaagaat tcgttaaact tggcaatttt
      481 gaatccgatt cagttgttca agggcacaaa gggaatattt tagaaagaat cattgagtat
      541 attgctggtt ccatgactcc aatcattcca gcaatgttag ggggaggtat gctgaaagtc
      601 ttggtaatta ttttaccaat gcttggtata ttgcaatcag attctcagac tattgctttt
      661 ttgacatttt ttggggatgt tccatattat ttcttaccgc tgttattagc ttattctgca
      721 tcacaaaaat taaaagtaac atctacatta gctatgtctg tagcaggtgt acttctccat
      781 ccaaattttg ttcaaatggt gcaatcaggg aatcctctta gtttatttgg tgtacctgtg
      841 acaccagcta gttatggttc atcagtcgtt ccaattctta ttatggtttg gttgatgaaa
      901 tatattgaaa aaatgattgc taaattaaca ccagctgtta ctaagagttt tttgcaacct
      961 acgctagtat tattagtatc aagctgtatt gccttagttg tagtcggacc tattggagta
     1021 attgttggtg aaggattatc aaatctagtt gggcaaatgt atggtgtagc tggatggctt
     1081 acattagcta ttcttggtgc tattatgcca tttattgtta tgactggaat gcattgggct
     1141 tttgcaccta tttttttggt ggcatctatt gctactccag acgtattaat tcttccagca
     1201 atgttagggt caaacttagc tcaaggggct gcttcgatgg ctgttgcatt aaagagtaaa
     1261 aataataata caaaacaaat tgcttttgca gcaggtttct cagccttact tgcaggtatt
     1321 accgaacctg cagtatatgg tgtgacttta aaatataaaa aaccgcttta tgcagctatg
     1381 attggtggtg gattagcggg attatttgca ggtcttacta gtgttaaagc gtatctattt
     1441 gctgtcccat ctttgatagc gttgcctcaa tttatttatt ctgatgtgcc atcaaatatt
     1501 gtaaatgctt taattgtggc ggtcatttcg gttgttatta cctttgtatt agcttatata
     1561 tttggaatcg atgaagaaga gagttctagc aatttagaag ttaaagctgg agtttcaaat
     1621 aaaaaaatga tattttctcc tatatcagga gaaatcattc cgttaagcga tgtccaggat
     1681 aaaacatttt cagataaact aattggagac ggagtagcga ttatcccaag tgaaggtaag
     1741 gtttatgcac catttgatgg gaaaattaca aatatttttc cgactaagca cgcaattgga
     1801 ttgaagagtg atgagggtgt tgagttacta attcatattg gattagatac tgttgagcta
     1861 aaaggtcaag gttttattag tcatgtagaa gaaggagaca gagttttcaa aaatcagttg
     1921 atttttgaaa tggacttgaa tttaatcaag actaaaggct acgaaacagt tacaccagta
     1981 attgtaacga ataccaatga ttttctagat gtattaatat tacctaataa tcagacaatc
     2041 gagcattcta aggaattact ggtaatatta taatggagga aagttatgac catttttcca
     2101 gatgattttc tttggggtgg agctgttgca gctaatcaag tagagggagc atataatgaa
     2161 gatggtaagg gcttatcagt tcaggatgtg ttacccaaag gtggattagg agaagcaaca
     2221 gaaaatccta cagaagataa cttaaaattg ataggtattg atttttatca taaatataag
     2281 gaagatatat ccttgttttc tgaaatgggc tttaatgttt ttcgtacttc tattgcatgg
     2341 agtagaattt ttccaaaagg agatgaggaa gaacctaacg aagctggatt gaaatattat
     2401 gatgaattgt ttgatgaact acatgctcac gggatagaac cacttgtaac tctttcacac
     2461 tatgagactc cattatattt agcaagaaaa tatcatggat ggattgatag gagaatgatt
     2521 catttttatg aaaaatttgc tcgaacagtt ttagaaaggt ataaagataa agttaaatat
     2581 tggcttacat ttaacgaagt aaactctgtt ttggaattac cgtttactag tggaggaata
     2641 gatataccta aggagaatct ttcgaaacaa gaattatatc aagctataca tcatgaatta
     2701 gtcgcctcga gtttggttac aaaaattgct cgtgagatta attcagagtt taaggtgggg
     2761 tgtatggtat tagctatgcc agcttatcca atgactccaa atccaaaaga tgtatgggcg
     2821 actcatgagt atgagaatct aaattatcta ttttcagatg tgcatgttag aggttattat
     2881 ccgaattatg caaaaagata ttttaaggaa catgacatta acatagagtt tgcagctgaa
     2941 gatgcaaagt tattaaaaaa ttataccgta gattttttat cctttagcta ttacatgagt
     3001 gtgactcaat ctgctcttcc aacacagtat aattcaggag aagggaatat tattggtggc
     3061 ttagtaaatc cttatttaga gtcttctgag tggggatggc aaattgatcc aattggacta
     3121 cgtataattt taaatagata ttatgaccgt tatcaaattc cattatttat agtggaaaac
     3181 ggattaggtg ctaaagatca actaataaaa gatgaattta ataacttaac agtccaagat
     3241 gattatagaa ttcagtatat gaaagaacat ttattgcaag tagctgaagc tctacaagat
     3301 ggtgtggaaa ttatgggcta tacgtcttgg ggatgtattg actgcgtttc tatgtccact
     3361 gcacaacttt ctaagaggta cggtctcatt tatgttgatc gaaatgatga tggaaatggc
     3421 acattcaatc gatataagaa aatgtccttt acttggtata aaggagtgat tgaatcaaat
     3481 ggagaatcct tattcaaata gtttgtttat tgatgattag atattatcac ggttaaggca
     3541 acatctcatt gaatatgaaa taaagaggtt tggaagacat caaattctaa acctctttat
     3601 tttatagggg gattaattaa tttaattttg ttacccccaa ttattcaccc ctaatctaaa
     3661 aaccatccag aatccttgcc ttagcttaga tcctggatgg tttctttttt cacccaatgg
     3721 gtgtttttta ctagacaaaa aagagtttcc cctttatggt ataagtgtag aaaaaaacac
     3781 aaaaagaaag gaaactcaca tgaacagttt accaaatcat cacttccaaa acaagtcttt
     3841 ttaccaacta tctttcgatg gaggtcattt aacccagtat ggtggtctta tcttttttca
     3901 ggaacttttt tcccagttga aactaaaaga gcagatttct aagtatttag taacgaatga
     3961 ccaacgccgc tactgtcgtt attcggattc agatatcctt gtccagttcc tctttcaact
     4021 gttaacaggc tatggaacgg actatgcttg taaagaattg tcagctgatg cctactttcc
     4081 aaaattgttg gaaggagggc agcttgcttc acagccaacc ttatcccgtt ttctttccag
     4141 aactgacgag gaaacagtcc atagtttgcg atgcctcaac cttgaattgg tcgaattctt
     4201 tttacagttt caccagctaa accaactcat tgtagatatc gattctaccc atttcacaac
     4261 ttatggcaag caagaaggtg ttgcttataa cgctcactat cgtgctcatg gctatcatcc
     4321 tctttatgct ttcgagggga agacaggtta ttgtttcaat gctcagcttc gtcctggtaa
     4381 tcgttattgt tctgaagagg cagacagctt tatcacacct gttttagaac ggtttaatca
     4441 acttctcttt cgaatggata gtggctttgc gacccccaaa ttatacgatt taattgaaaa
     4501 aacagggcaa tactacctca taaaactcaa gaaaaatact gttctgagcc gtcttggaga
     4561 cctttccctc ccttgcccac aggatgagga cttaaccata ttctaggatt agaaaagtca
     4621 acctgaatct tcctgttcct tatgaaccac ctagaagaaa agcgtcgtta atgatgcatt
     4681 aaagaacagt cgagatgaaa aaatcgtgtg acgcaccaag ggaggagtct gcccttttga
     4741 ggaaatctag cgaggaaaaa cgatactgga acagcagaaa gtaaaactga cctcatgagg
     4801 aggaagaaag tggctcatga ggtcaggggt tttgtaagtt acatctagtt gagagaggta
     4861 tgaatgattt ggggttacag agagccgatt ataaatagtt aacttaggat agattggtta
     4921 t
//
LOCUS       DAVKAO010000093         4888 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKAO010000093 DAVKAO010000000
VERSION     DAVKAO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.093"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            331..513
                     /locus_tag="VWX16_001881"
                     /pseudo
     CDS             331..513
                     /locus_tag="VWX16_001881"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1125..1409
                     /locus_tag="VWX16_001882"
     CDS             1125..1409
                     /locus_tag="VWX16_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159179.1"
                     /translation="MDWYDYMIQASKQSQFNARHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1402..1959
                     /locus_tag="VWX16_001883"
     CDS             1402..1959
                     /locus_tag="VWX16_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter peripheral membrane
                     component"
                     /protein_id="HEU9159180.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNTHEELWLNNHVANMNKQPPLSLCESLYSFENLTVL
                     VVPIEYVLGMKMMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVLLEG
                     FSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(2326..4650)
                     /gene="pflB"
                     /locus_tag="VWX16_001884"
     CDS             complement(2326..4650)
                     /gene="pflB"
                     /locus_tag="VWX16_001884"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9159181.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDKYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1582 a          888 c          892 g         1526 t
ORIGIN      
        1 tttagcgcca catagtactg aactctctcc aaaaatggtt attcctcttt gaatagggcg
       61 ttatcacaag aaaagcatct ccacgtttca acttcatatg gctcaaaaac aatcaattga
      121 tgctaaaacc tgtacctaga tgtttcggtt cataaaacca tgaaactgta aaagtggatg
      181 aaattgatag cgatagtcaa atcaagaggc atcataactc taaaaagtca caatatataa
      241 gttcatcctc ggaaaaatat cattctaatt gttgaaatgg atatataaac aggggtatta
      301 acattaaatg ctcatgaagc aacgaataca ggtatcaaaa ctatgacaaa acaagtccct
      361 aaatttacta aagacactgc tcaactttac acctgtaaat ggttgttgta taataaagtt
      421 acaaagatgt acgaccacac cgttgtaaat catagtgttc gcgaatatat tactgatagc
      481 atttctacaa atacaattga agaggctgga tagggtttaa acgaaatatg tcagtgtttt
      541 ggcattccta gccttcatat catttaaaga attgtataga caaaattttt cccaatacag
      601 acactcgtaa caactgcttc atttttctac caacatattt aggaacagga taagatacaa
      661 gaggattaat ccatagatca gttctatacc aatctaagac aaataagcta aaaaaacgat
      721 tgataataag caaatagatt ccaaattttc tctatctgct cattttaata aacaatacta
      781 gtgtaactat ccttccagtc agaagcttgt caaatcacac cgaaaattct tctaaaattt
      841 atctcgttag gcaatcaagc aaaaactcga cgatagtaca aacattatca tacagaattg
      901 acttcctaaa ttatatactt tagtaaggtt ttcggataag aaaaaagatt cattttacat
      961 ttctaaacat tcttttctaa gatgaaaaac agaatttttc gattgtgatt taaagcaaca
     1021 agaagatttt cagtatcatc ctatagatac gagctaatta agaaaaacta catttttgaa
     1081 tataaactac aataagataa actaaatttc ataggaggaa gacaatggat tggtacgatt
     1141 atatgataca ggcatccaaa caatcacaat tcaacgcaag gcattggttt cgctatttgc
     1201 gaaaagttat ttttgaagac tattcttatt taacaaacca agatgtagaa aagttgctag
     1261 actccaaaga actaacccgt tttcaaaaaa ttagcttgaa gtatgccttt caagagcata
     1321 ctccaactca taaatatgtg atttcattaa ataaacctgc taagttaacc aatgttcaaa
     1381 aattgatgga gaaatacaaa catggataaa atgaaaccgg tcttccaagc cctaaataag
     1441 gaattaattc aggaaaatct gactttaaca attatctgtg tcggtggtta tgtattagaa
     1501 tatcatggtt tacgtgccac acaagatgtt gatgcttttt atgaccaaaa tcagaaaatc
     1561 aatgaaatta ttgctcgtgt aggaaaacag tttaatctta acacccacga agagttatgg
     1621 ctcaacaatc atgttgcaaa tatgaacaaa caacctccct taagtttatg cgaatccttg
     1681 tactcatttg agaatctgac cgttttagta gtccctattg agtatgtgtt aggaatgaag
     1741 atgatgagta tcagagagca ggatttgaaa gatattggtg caattattaa atacaaaaac
     1801 tttcattcac cattcgatac ctttaaatat ttaaaagaca tgggatttga tactattgac
     1861 ctctctgttc tattagaagg ttttagttat gcttatggta tggattggtt agaaaaattt
     1921 tttaaagaaa atcaagataa acttcgcgaa ttttattaac agtatcaaac aagatgattg
     1981 tcaattagcc agtcgtcttg tttttatgat ggaatgaaat aaaatcggaa caaatcactt
     2041 agagaattcc agtcaatttc tagcgctgtt tgagacgttt cagcggacta tagtaaaatg
     2101 aaataagaat aggacaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
     2161 ataaagatgt actattttag tttcaatcta ttatatttca gattcgatcc actattcctt
     2221 cccaaaaaca ttgcatattg cgtcaaagga attgctcaca ctatcccaag cacaaaaccc
     2281 tcagtcgttt gaccgagggt tcctttttat tattcaagaa cttgattagc tcaatgcatc
     2341 caaggcgtca tccattgaaa gaacttcgtg gaagacacgt tgtgtcaatt cagttttttg
     2401 ttctggagtg aggtatttag tgtttacaca gtatccagag atacgtacga taacgtcttc
     2461 gcctgacatg attttttcgt aaacatcgtt caagtccata acgttcaagt taacgtgttg
     2521 tccaccgttt tcgaagtaac catcaaggat tgttaccaag ttatcaactt gttcatcacg
     2581 agtcttacca agagcgcgag gtgatacttg tgtagtcaat gagataccgt cagctgcata
     2641 actaaaatca aggctagaaa gtgagttcaa gttttgcaac caaccacctt tagctttgtt
     2701 agatgggtta gcacctggtg agaagaattc aagtttagac aagttcacag aaccatcttc
     2761 gttgaggtat acacctttgt gaactggtga gttaccagtt tgtttagagt aagcaacgtt
     2821 agatgtgatt gtcaaaagtg atactgtagc ttctgcgtct ttgtatagtt tgtggctacg
     2881 tagacgagtt gtgtaagctt cgatcaacca ttctgccaat tcgtttgaac gtgggtcatc
     2941 ttcaccccag cgtgggtagt caccgattgt ttcgtaatcg tagatgtagc catcttcatc
     3001 acggattggt ttaactgtag cgtatttaat cgctgacaat gtatcaacag tgttagcaaa
     3061 tccacaaata ccgaatccca tgttggcacg ttgtttagtt ggcaagaagg ccatttgaac
     3121 agcttcgtag ttgtacttat cagtcatgta gtggatgatg ttcaaggcat ctacgtaagt
     3181 gtcagtcaac cagtcaagag atttttcaaa gttcgcttta actgattcaa attcaagaac
     3241 ttcgtcacga ataggatcga tgtcaaatac tttgtagtct ttgtgaacat cgtcgtaacc
     3301 accattcaaa ccagtaagaa gggctttaag aacgtttaca cgagcaccga agtactggat
     3361 gttgtggcgt tgttcttcat tttctggatc aagtggagac acacagcatg agatacagct
     3421 catttcacca tatccgtctt tagccattgt tgttacacct tcgtattgga tagaagagtg
     3481 tttgtggctc atgtgcatac agtagcgacg gaagttgtat ggcaatttgt cagtccaaag
     3541 aactgtcaag tttggctctg gagagttacc tatgttgtca agagtgttca agaaacggta
     3601 gtccatctta gtaacacggt gacgaccgtc gttacccata ccagccatag aagttgtgat
     3661 gaatgttggg tcacctgagt acaattggtc ataagctttt gtacgagcaa atttaactgt
     3721 acgaagtttc ataacgaaat catcaacgaa ttcttggatt tctgattcag taaatgtacc
     3781 acgagcaagg tcacgttctg caaagatgtc caatacgatt ggtacacgac ctagagatgt
     3841 agcagcaccg ttaatcacac ggcagacagc catgaaagca atgttaaccc attggattgc
     3901 ttctttcacg ttcatcgctg gtttgcgaac atcaaccccg taaaggtcac ccaggcgaac
     3961 aacttgttgc aatgcttggt attgaaggtt tacttcttca cgaagacgga ttgtttcttc
     4021 atcgatttct ttgattgcat tccagtcatt tactttttct tgcatcaagt agtctgcacc
     4081 gtaaagagca agacgtgcgt aaacaccgat gatacgtccg cgtgagtatg catctggaag
     4141 accagttaca gtgtgtgcgt gacgagcgcg acgaatattt gaagtgtagg cacggaaaat
     4201 accgtcgtta actgttgtta catatttagt gaagatttcg tgaacagctg ggtctggttc
     4261 gtatccattt tctttcaaag tagtttcagc catacggata ccaccttttg gcatgaagtt
     4321 caatttgaag agttcatcgt tttggatacc gaagataact tcattttctt tgtcgataaa
     4381 tccagcaggg atatcagcga tagatgttgg acgagtgtcc attgggaaac gagtttcttc
     4441 gtagtgtgct ttagtttctt ctacaatttt cttgatgtga agtgaacgct ctgttggtcc
     4501 tgcaaggaag ctttcgtctc catcataagg tgtgtagtta gcttgtacaa agcgtgatac
     4561 acttgctttt tctttccaat ctacgccttt gaagccttcc caagctttgt caaaaatatc
     4621 ttgtgcttca acaactgtct taacaaccat attaatgtcc tcttttttct agtaacagtc
     4681 atctgttaca ttcatgagac aagtatacca tatagtaacc gatttcaaca agtgaaaaat
     4741 ccctattttt acactttctt ttctaaaaca gtctatattt tatttcaaac tgtattatat
     4801 ttttgaaaaa ataaagtcct tttttctttt tttcagaaaa aagggtataa taaaagaaaa
     4861 taagcagtaa cactcaatgg aaatcgaa
//
LOCUS       DAVKAO010000094         4870 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKAO010000094 DAVKAO010000000
VERSION     DAVKAO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.094"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..107)
                     /locus_tag="VWX16_001885"
     CDS             complement(<1..107)
                     /locus_tag="VWX16_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComD"
                     /protein_id="HEU9159182.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHLICKGQINRKELFV"
     gene            complement(128..253)
                     /gene="comC"
                     /locus_tag="VWX16_001886"
     CDS             complement(128..253)
                     /gene="comC"
                     /locus_tag="VWX16_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9159183.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(419..492)
                     /locus_tag="VWX16_001887"
     tRNA            complement(419..492)
                     /locus_tag="VWX16_001887"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Arg,seq:tct)
     gene            complement(536..1015)
                     /gene="rlmH"
                     /locus_tag="VWX16_001888"
     CDS             complement(536..1015)
                     /gene="rlmH"
                     /locus_tag="VWX16_001888"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9159184.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            1198..2379
                     /locus_tag="VWX16_001889"
     CDS             1198..2379
                     /locus_tag="VWX16_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU9159185.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            2437..3195
                     /locus_tag="VWX16_001890"
     CDS             2437..3195
                     /locus_tag="VWX16_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9159186.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            3408..4769
                     /gene="dnaA"
                     /locus_tag="VWX16_001891"
     CDS             3408..4769
                     /gene="dnaA"
                     /locus_tag="VWX16_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9159187.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1702 a          868 c          805 g         1495 t
ORIGIN      
        1 aaaacgaata attcttttct atttatttga cctttacaaa ttaaatggta gctgtgacta
       61 ataattaaaa aatgaacaat aactgtccca aatccaaata aatccattac tctttcccct
      121 tatttcatta cttttttctt tgtaaaataa aatcacggaa gaattttgac agcctcatct
      181 ccccaccttt aatcttttgt aagtcttttt ccttcaaagc tacaaactgt tccaatttaa
      241 ctgtgttttt cataataaaa tctcctaaaa tgtttttttc ttgtaagcta acttacaaaa
      301 accattatac aaaatggaat ttcgttttag ataaaattct ctcaactgtc atttttttct
      361 cccaaagtgt acttttttaa gaaaaaagcc gggaaaattc ccagctttgc tattatattg
      421 atcccagcag gattcgaacc tgcgaccgtt cgcttagaag gcgaatgctc tatccagctg
      481 agctatgaga cctaacacaa ttattctacc aaaaattcaa ttaaaagtca attttctatt
      541 tatggtaggg gaatccctgc tgaatcgtaa aagcgcgata gatttgttca acaagaacta
      601 gtctcattaa ctgatgaggc aaggttaggc gaccaaaact gacagaaaga ttggctctat
      661 tttttacaga tgatgacaat cctaaacttc ccccaataat aaaagtaaga gtagaaaatc
      721 cttttataga agtttcttct aactgcttac taaattcttc tgagaagaaa gttttccctt
      781 caatggctaa cacaataacg aaatcacggt cagcaatttt tgataaaatt ctctgacctt
      841 ctatttctaa aatcttttga ttttctgatt cactggcctt atctggtgtt ttttcatctg
      901 ataactcaat catttcaaac ttagcaaatc tagaaattcg ttttgaatac tctgcgatac
      961 catcttttaa atacttttct ttcagtttcc caactgttac aactttaatt ttcatgactc
     1021 tattctaaca tattctctat tttttcacat cttattcaca aaataaaaaa tagatttcaa
     1081 ttaagaaaat cacagtttca aaagagttat ccacagtttg tgtaaaactt ttgtgtttaa
     1141 gttataatta agctagtcag tttatacttt cagtaattca aacatatgga ggcaaatatg
     1201 aaacatctaa aaacatttta caaaaaatgg tttcaattat tagtcgttat cgtcattagc
     1261 ttttttagtg gagccttggg tagtttttca ataactcaac taactcaaaa aagtagtgta
     1321 aacaactcta acaacaatag tactattaca caaactgcct ataagaacga aaattcaaca
     1381 acacaggccg ttaacaaagt aaaagatgct gttgtttctg ttattactta ttcggcaaac
     1441 agacaaaata gcgtatttgg caatgatgat actgacacag attctcagcg aatctctagt
     1501 gaaggatctg gagttattta taaaaagaat gataaagaag cttacatcgt caccaacaat
     1561 cacgttataa atggcgccag caaagtagat attcgattgt cagatgggac taaagtacct
     1621 ggagaaattg tcggagctga cactttctct gatattgctg tcgtcaaaat ctcttcagaa
     1681 aaagtgacaa cagtagctga gtttggtgat tctagtaagt taactgtagg agaaactgct
     1741 attgccatcg gtagcccgtt aggttctgaa tatgcaaata ctgtcactca aggtatcgta
     1801 tccagtctca atagaaatgt atccttaaaa tcggaagatg gacaagctat ttctacaaaa
     1861 gccatccaaa ctgatactgc tattaaccca ggtaactctg gcggcccact gatcaatatt
     1921 caagggcagg ttatcggaat tacctcaagt aaaattgcta caaatggagg aacatctgta
     1981 gaaggtcttg gtttcgcaat tcctgcaaat gatgctatca atattattga acagttagaa
     2041 aaaaacggaa aagtgacgcg tccagctttg ggaatccaga tggttaattt atctaatgtg
     2101 agtacaagcg acatcagaag actcaatatt ccaagtaatg ttacatctgg tgtaattgtt
     2161 cgttcggtac aaagtaatat gcctgccaat ggtcaccttg aaaaatacga tgtaattaca
     2221 aaagtagatg acaaagagat tgcttcatca acagacttac aaagtgctct ttacaaccat
     2281 tctatcggag acaccattaa gataacctac tatcgtaacg ggaaagaaga aactacctct
     2341 atcaaactta acaagagttc aggtgattta gaatcttaat tgacatctat gtaaagaaag
     2401 ctttacataa gagaaaagat gtgttagtgt agaatcatgg aaaaatttga aatgatttct
     2461 atcacagata tacaaaaaaa tccctatcaa ccccgaaaag aatttgatag agaaaagcta
     2521 gatgaactag cacagtctat caaagaaaat ggggtcattc aaccgattat tgttcgtcaa
     2581 tctcctgtta ttggttatga aatccttgca ggagagagac gctatcgggc ttcactttta
     2641 gctggtctac ggtctatccc agctgttgtt aaacagattt cagaccaaga gatgatggtc
     2701 cagtccatta ttgaaaattt acagagagaa aatttaaacc caatagaaga agcacgcgcc
     2761 tatgaatctc tcgtagagaa aggattcacc catgctgaaa ttgcagataa gatgggcaag
     2821 tctcgtccat atatcagcaa ctccattcgt ttactttcct tgccagaaca gattctctca
     2881 gaagtagaaa atggcaaact atcacaagcc catgcgcgtt ccctagttgg gttaaataag
     2941 gaacaacaag actatttctt tcaacggatt atagaagaag atatttctgt aaggaagtta
     3001 gaaactcttc tgacagagaa aaaacaaaag aaacagcaaa aaactaatca tttcatacaa
     3061 aatgaagaaa aacagttaag aaaactactc ggattagatg tagaaattaa actatctaaa
     3121 aaagacagtg gaaaaatcat tatttctttt tcaaatcaag aagaatatag tagaattatc
     3181 aacagcctga aataaggctg ttcttttatt tttttatctc acaaggttat ccactatgtt
     3241 tttcgataaa aagcttaata aatcaataat ttcttctttt atccccaacc tgtggataaa
     3301 gtttggtaac attgtggatt atttttcaca gcttgtggaa aattcttgct atctatggta
     3361 aaatatctct agtattaaac ttttaaatag taaaggagga gaaaggattg aaagaaaaac
     3421 aattttggaa tcgtatatta gaatttgcac aagaaagact gactcgatcc atgtatgatt
     3481 tctatgctat tcaagctgaa ctcatcaagg tagaggaaaa tgttgccact atatttctac
     3541 ctcgctctga aatggaaatg gtctgggaaa aacaactaaa agatattatt gtagtagctg
     3601 gttttgaaat ttatgacgct gaaataactc cccactatat tttcaccaaa cctcaagata
     3661 cgactagctc acaagttgaa gaagctacaa atttaactct ttatgactat agtccaaagt
     3721 tagtatctat tccttattca gatacgggat taaaagaaaa gtataccttt gataacttta
     3781 ttcaagggga tggaaatgtt tgggctgtat cagccgcttt agctgtctct gaagatttgg
     3841 ctctgaccta taaccctctt tttatctatg gaggaccagg ccttggtaag actcacttat
     3901 taaacgctat tggaaatgaa attctaaaaa atattcctaa tgcgcgtgtt aaatatatcc
     3961 ctgccgaaag ctttattaat gactttcttg atcacctaag acttggggaa atggaaaagt
     4021 ttaaaaagac ctatcgtagt cttgatcttt tgttaatcga tgatatacag tcactcagcg
     4081 gaaaaaaagt cgcaactcag gaagaatttt tcaatacctt taacgccctt catgacaagc
     4141 aaaaacagat tgtcctaacg agtgatcgta gtccaaaaca tctagaaggg ctcgaggaga
     4201 ggcttgtcac gcgttttagt tggggattga cacaaactat cacaccccct gactttgaaa
     4261 cacgtattgc cattttacaa agtaagacgg aacatttagg ctacaatttc caaagtgata
     4321 ctctagaata cctagctggg caatttgatt caaatgttcg agatcttgag ggagccatca
     4381 acgacatcac tttaattgcc agagtaaaaa aaatcaagga tatcactatt gatattgctg
     4441 cagaagccat tagagcccgc aaacaagatg ttagccaaat gctcgtcatc ccaattgata
     4501 aaatccaaac tgaagttggt aacttttatg gtgttagtat caaagaaatg aagggaagta
     4561 gacgccttca aaatattgtt ttggcccgtc aagtagccat gtatttatct agagaactaa
     4621 cagataatag tcttccaaaa attgggaagg aatttggggg aaaagatcat accacagtca
     4681 ttcatgccca tgccaaaata aaatctttga ttgatcaaga cgataattta cgtttagaaa
     4741 ttgaatcaat caaaaagaaa atcaaataat ttgtggataa cttttagttt tttatctttt
     4801 ttatccacat tttttaaaca agctaaaaaa cttgatatga cttgtttaaa ggctgttttc
     4861 cacagatttc
//
LOCUS       DAVKAO010000095         4643 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKAO010000095 DAVKAO010000000
VERSION     DAVKAO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.095"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            106..1284
                     /locus_tag="VWX16_001892"
     CDS             106..1284
                     /locus_tag="VWX16_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9159188.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDTNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGEAWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(1512..1958)
                     /locus_tag="VWX16_001893"
     CDS             complement(1512..1958)
                     /locus_tag="VWX16_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9159189.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            2028..2687
                     /locus_tag="VWX16_001894"
     CDS             2028..2687
                     /locus_tag="VWX16_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9159190.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWKLFSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILITSSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(2739..3890)
                     /locus_tag="VWX16_001895"
     CDS             complement(2739..3890)
                     /locus_tag="VWX16_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9159191.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDNQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(3905..4636)
                     /locus_tag="VWX16_001896"
     CDS             complement(3905..4636)
                     /locus_tag="VWX16_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159192.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
BASE COUNT         1436 a          918 c          691 g         1598 t
ORIGIN      
        1 agagaccagt aagtaatgaa aagcttaaca agattaacat ctacatttat ccatagcaaa
       61 aaatcatagg attttaagaa tctgacaact aggaagaaaa ctaatatgac cttatctaag
      121 aaacaattac aactaagagc taaaatactg gaaacagttt acacattagg tcccatttca
      181 cgaattgaaa tcgctactaa aacaggaata acacctgcaa caactagcag tatcacaaat
      241 gatctgatta aagaaaatat tctcttagaa cttggtgaag acgaacacga tacaagcgta
      301 gggagaaaaa aaatcctgct agacattcaa gctaaacgat tttactacat tggttgtgaa
      361 ctatctgaaa aacatttcac ttttgcttta ggagataatc taggaaatat cttaaaggaa
      421 gagaaagaaa ttgttactaa acagttaatc caagaaaagg gaaatcaact cattaaccag
      481 acactaaagc aattcttaaa taactgctct gattatgaaa tagaagcaat tggaatcgcg
      541 ctacctggtc gctatttaga tgattataaa ataacaacaa acaatccttt atggcaacat
      601 atcgacttag aaatgattca atcccacttt gataagcctt tgtttttctc taacaatgtt
      661 aactgtatgg ctataggtaa acgcctattt agtcgccaac agaatgacac taattttgct
      721 tattttcatt ttgctagagg tatgcactgt tcttatatct acgacggaaa catttatggg
      781 aaaggaaatc taatgattgg agaaataggg catacagttg tttcttctga gggagaagaa
      841 tgtagctgcg ggcgcaaggg atgcttacaa acttttgctg gagaagcttg gctaatcaaa
      901 aaatcaaaaa ttctatatca tcaatctccc tattctctac ttcctagttt agtcaaaaat
      961 gccgatgata ttgatatcca agttatttta accgcttatc aattaggtga cactggaata
     1021 atcacactta tccatcaagc cttactctat cttagtcaaa ccattttaaa tatcagtatg
     1081 atgattgact ctcaaaaaat ctacctacat agtcctttac taacaaatca acacatcatc
     1141 caaaaactat atagtgaaat gaactacaaa cccaaactgc tctataatcg cctacctgaa
     1201 gtcattattg aaccttacaa tgattttaca gccgcacact cagctattgc actttgtttg
     1261 tatcatacaa ttctacattc ttaattttct attacattta ataaataggc taaaaagagg
     1321 gagtatatca taaaaagcga acaaacctgt tttctgaaaa tcagaactct gttttgttcg
     1381 ctttctttat atttggaatt atactcctat ctcccctttt tcacaatgta tagtaaaatg
     1441 aaataataac aggacgaatc gatcgggaca gtcaaatcga tttctaacaa tgttttagaa
     1501 gtagaggtgt actattctag tttcaatcta ctatattttc gtacaggtgc ttcaaccatt
     1561 tgaacgattt caaatccttc tttttggtaa agattctgag ctctttgatt tgcctcgaag
     1621 acatttagag aaatactatc tatatcttca ttttcaaagg ccaaactaac aaatttcctt
     1681 aaagcctggc tacctaagtc ttgctcctgt ttctgggggt tgataaaaaa tctcccgata
     1741 tgaagattgc tgtcttctag cctgattttc tggataagtc ccacaaactc ttgttcatca
     1801 aagattgaaa agactccttc caaggcttga agggtcagta gaaaaggaat ccttggtccc
     1861 atccattgtt cttgaaagga tttgcctagg gagttggacc actggcatac aaattgagcg
     1921 ttttctatac tcaccttttc ttcaaaacga attgtcatct tttcctcacc atcttatcta
     1981 tgtttctcca ttatactatt tctcccattt tttacgaata gataagtatg attgattttt
     2041 atttttttct cgtcgggagc attctcgctt cctttcttgg tttggtcatt gaccgttttc
     2101 cagagcaatc cattatcagt tcagccagtc actgcgattc ctgtcagact cgcttgcgtc
     2161 ccttagattt gattccgatt ctctcacagg tcttcaatcg ctttcgctgt cgctactgca
     2221 aagttcgcta tcctgtctgg tatgccctct ttgaattagt cttaggactc ctctttctgc
     2281 tttactcttg gaaattgttt tccttggggc aagtcgtcct aatcaccgct ggtttgacct
     2341 tgggtatcta cgactttcac catcaggaat atcccttact ggtctggatg actttccacc
     2401 taatcctaat aacttcctct ggctggaatc tggtcatggt ctccttcctc atacttggaa
     2461 ttttggctca ttttatcgat atccgcatgg gcgcagggga tttcctcttt ttagcttctt
     2521 gtgctctcgt ctttagcgta acggagttac tgatcttgat tcagtttgct tctgcgacgg
     2581 gtatcctggc ctttatcctg caaaagaaaa aggaaagact tcctttcgtg cctttcctct
     2641 tacttgctac ttgtttgatt atttttggta agctactgct tgtctgataa aatccaattt
     2701 ctgccatata tccttcatga aattatttca cagttaaatt ataaattatt tcttttgtac
     2761 aaagggatga tgttatcaaa tcgatctgtt cttctatctt cttgatactg atcaaaaaat
     2821 ttcatttcga ctgaaaatat ttcgcttata aactgtaaac gaatactttg tttagacatt
     2881 atagtcgcta gactgactag atgattactc aaaacgacgt ccagaatact ctttactttg
     2941 cttggttttt taacaaaaat ttgattatcc aaaggttcaa tcattttgta acctttttgc
     3001 gcaatttgac gataaaagta agaatgttgc tttggagtca ataatcctaa cttaaaagct
     3061 cgatactcta aagcctgtat cgaaacattc aaatccgact tcaataaaat ataactatca
     3121 ggattgctga cacgcttgcc aaccctctct tcaaatttga ctaaaaactc ttcttttggc
     3181 aataaaaaac atgatgcaaa ataatttgct tcttgctcca aacgatcgcc atcttcattc
     3241 atatctttat atttatgtaa aagaatatgt cctagctcat gagctaagtc aaaatttcga
     3301 cgtacagatg atttattcgt tcccaacaca atataaggtc ttcccaattt tgaccatgcg
     3361 ctataagcat cagcttggcc attaattaat cgttccacga tatagatgcc tgagcgttct
     3421 aatttataaa gcaaatcatg attatctttt gaaataccta atttttccct ggcataaaga
     3481 gccaattcct caatggattc tcccttatga taagattcac tcactacatt acttaggtca
     3541 tgaattgtaa tattaggtat aattacaaaa ctttcaaaat aatcaatcaa actatctacc
     3601 ttatgtaaat acatagtttg aatatctatt gttttccgtg ttgctaggtc tgcatttcta
     3661 aaggcaatta cagaagaatc aaatcgaatg ctctcttctt cctgttcaaa ataagttaaa
     3721 tcaacatgaa attggttggc caaatgcatt ttggttgata atttaggttt cgtttcgttg
     3781 gactcaaact gccaaatggc ttgttccgtt aaattaattc tctgagctaa ttctgctcta
     3841 cttaaaccat ttaacagccg taattctttc aatacccgac cattaaacat ttacatactc
     3901 cttactactt ttgaccttct tgtttttcta ttcttggaat aatttcaaaa tcttctgttt
     3961 ccgataattc tgaaaaatta ggaatatctt gatatttagc ttcttcgaaa tggtacgaac
     4021 ttgattgaat atactcagac aaatcttgaa tcaagtgtaa ctgaccagtt cgtgcatcag
     4081 gcataacaac atctacagat ataatattgt tttctgagtc cgcctcataa gttaaaatca
     4141 taaatttttc gatattcgaa tttttagtag cttgttcaat ttcttgaatc atttcatcag
     4201 aaactaactc catctgaatt ggaaaggaat gactatcttc atcatttttg taggaagaat
     4261 gttgattaag ataaagtgta ttcatctgag catattcaaa taagtagcca ctcttatttt
     4321 tttgtaccaa aggaaattgg ttcgtaagtc gcttcttacc ctttataatt aacaatactt
     4381 tcccatattt ttctgtattt gtttcaaatt ctaaatatcc ccaagtctgt cctgctaatt
     4441 gtaatttata ctcaaacaaa tctgctgatg caaatgcagt atcaatatga ttaggtcgcg
     4501 tccatgcata accattcgac actatcattg tctctctttt ttctagacgt tcatctacat
     4561 aatctttttg ccctttcatc aaagtatcta caattttttg tgcctcaagc gaatcaaaga
     4621 gatcctgatt caacataatt ctt
//
LOCUS       DAVKAO010000096         4484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKAO010000096 DAVKAO010000000
VERSION     DAVKAO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.096"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..631)
                     /locus_tag="VWX16_001897"
     CDS             complement(<1..631)
                     /locus_tag="VWX16_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9159193.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADH"
     gene            complement(813..1775)
                     /gene="nrdF"
                     /locus_tag="VWX16_001898"
     CDS             complement(813..1775)
                     /gene="nrdF"
                     /locus_tag="VWX16_001898"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9159194.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(1963..4122)
                     /gene="nrdE"
                     /locus_tag="VWX16_001899"
     CDS             complement(1963..4122)
                     /gene="nrdE"
                     /locus_tag="VWX16_001899"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9159195.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4203..4421)
                     /gene="nrdH"
                     /locus_tag="VWX16_001900"
     CDS             complement(4203..4421)
                     /gene="nrdH"
                     /locus_tag="VWX16_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9159196.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         1300 a          838 c          916 g         1430 t
ORIGIN      
        1 tatgatctgc gagtaaatat tttttattag aattatttaa agcgatgcgt tgagcctctc
       61 cctcttcctc gctaaaagta gctagagctc cgttttgaat accattacag ctaacgaaag
      121 ctttagaaaa ttggagatta gagagatttt gtagggtcaa tgtgccaaca aaagcacctg
      181 taatatcgcg ataatttcca cctatcaaaa tcaaatctgt taattttcgt tcgcttaaaa
      241 tcagaaaaac aggtagactg ttggttacga cgcggatatt gtcaataggc aactcacgcg
      301 caaaaaactc taatgttgtt cctggtccaa tgaaaatagt ttctctttct tctactagac
      361 tgcctgcaaa atgggctatt tcttgttttt ctgccgtttg gagggcttgt ttttcaatat
      421 ttgatcgctc attagtcaaa agggagttgg ttcgaagttt ttcagctcca ccatgcacac
      481 gaatcagcaa atctttatca gctaattcct gtaaatagcg ccttgcagtc atatctgaaa
      541 cggctatttc gtccataatc tgtttaactg ttatggttcc tttactattt actatctcta
      601 aaattttggt taatttttct tgtttgagca tattatcacc tcgtttccta ctactatctt
      661 accataaaca aactcatcat tcaaatacaa aaacaacaaa atgaaacaaa aacaaaaata
      721 tcgaagtttg ttttcaaaac tttcgatatt tttgttgggt tataactttg atgtttctag
      781 tttacttttt gatgattgag agtgatggag aattagtcta aaccgtagtt atagtcatcg
      841 tcttgcatgg cttcaacttc gccaagaagg taaccatttc cgacttgaga gaagaagtca
      901 tggttggaag ttcctgttga aataccgttc ataacgattg ggttgacatc ttcagctgaa
      961 tctgggaaaa gtggatcttg tcccatgttc atgagagctt tattggcatt gtagcgaagg
     1021 aaggttttaa cctcttcagt ccaaccaaca ccgtcataaa gactctctgt gtagccttct
     1081 tcattttcat aaagagtata gagtaggtcg tacatccatt ctttgagttt ttcttgctct
     1141 tcttcaggca attcattgaa accaagttgg aatttgtaac caatgtaggt tccgtgaaca
     1201 gactcgtcac gaataatcaa tttaatgatt tctgcaacgt tggctagttt gttgttaccg
     1261 agatagtaga ggggagtgaa gaaaccagag tagaagagga aggtttcgag gaagacgctg
     1321 gcaactttct tttcaagtgg gctgccgttt aggtagattt cgttgacaat ctcagccttc
     1381 ttttgtaggt aaggattggt attggtccat tcgaaaattt cttcaatctc agccttagta
     1441 ttcaaggtag aaaagattga tgagtaagat ttagcgtgga cagattccat aaattggatg
     1501 ttattgaaaa cagcttcctc atgtggtgta cggatgtctg cgcgaagggc ttgaacccca
     1561 gtttcagatt gcatagtgtc aagaagggtt aaaccaccaa aaacttttcc gaccaagtct
     1621 ttctctttgt tagatagctt tctccagtca tccaagtcgt ttgataaggg aatacgtgta
     1681 tcgagccaaa attgctccgt cagtttttcc caagttgatt tgtcgatgac atcttcgatg
     1741 gcattccagt taatggcttt gtagtaagtt tccatttaaa atctctttct gtgtttagta
     1801 ttgcgaactc acaattattt ctactttacc ataattctat aggagtatcg cacaaaaagt
     1861 cggaagcccg acttttaaaa tgttacataa attatgttat gacatagtag atttgatttt
     1921 atctgtgctg cttagggaaa aataatgttt ctatgctaga aactaaatca cacagctttc
     1981 acattggttg gcgccgactt ctccaccgtc atctgtaaag gtacggacgt agtagataga
     2041 cttgattccc ttgttaaagg catagttacg aaggatggac aagtcacgtg tcgtttgttt
     2101 attttccctc ttccattcgt aaaggccttt tggaatgtca ctacgcatga agagggtgag
     2161 tgaaagtcct tgatccacgt gttcagtcgc agcagcgtaa acatcgatga ctttacgcat
     2221 atccatatcg taggcagaag tgtagtaagg aatggtttct gtagacaagc cagcagcagg
     2281 gtagtagatt ttaccaattt tcttctcttg gcgttcttcg atacgttgcg taatcgggtg
     2341 gatagaagca gaaacgtcgt tgatatagct gatagaacca tttggcgcta cagcaaggcg
     2401 attttggtgg taaagaccat cttcttgaac cttgtcgcga agttcagccc aatcagcaac
     2461 accagggata aagacatttt tgaagagttc tttaacacgg tctgatgttg gaacaaattc
     2521 accagttaca tacttgtcaa agtaacttcc gttagcatag tctgattttt caaagttgtg
     2581 gaaggtaata ccacgttcac gcgcaatatt gtttgactct accaaggtcc agtagttcat
     2641 aagcataaag tagatgcttg taaattcaac agactcaggt gaaccatatt caatgagttg
     2701 ttgggcaagg tagctgtgca gtcccatggc accgagacca aaggtgtggg cttggctatt
     2761 tccatggtca atcgttggta cagctacgat atgtgaacta tctgtaacga aagtaagggc
     2821 acgaaccata gcacggatag aacgaccaaa atcaggtgaa gtcatcatgt taaccacgtt
     2881 ggttgaaccc aggttacatg aaacatctgt tcccatttga aggaattctt gagcatcgtt
     2941 gatcaagctt ggttcttgaa cttgaagaat ctcagaacac aagttactca tgataatctt
     3001 tccatcaaca ggatttgcac ggttagccgt atctatgttg actacataag gatagccaga
     3061 ctcttgttgc aatttagaga tttcagtttc caaatcccgc gccttgattt ttgtcttgcg
     3121 aatatttgga tttgcgacca attcatcgta tttttcagta atgtcgatgt aattgaatgg
     3181 cacaccgtat tctttttcta cagagtaagg gctgaagagg tacatttctt catttttacg
     3241 agccaattcg tagaatttat caggtactac aacaccaagt gatagagtct tgacacgtac
     3301 tttttcatca gcgttttctt tcttagttga aaggaaagcg atgatatctg ggtgaaagac
     3361 gttgaggtag acaacaccgg caccttgacg ttgccccaat tggttggagt aagagaagct
     3421 gtcttcaaaa agcttcataa caggaacgac acctgaagca gctccttcat agcctttgat
     3481 aggtgcacca gcttcacgaa ggttgctgag ggtaattccc acaccaccac caatacgtga
     3541 aagttgaaga gctgagttga tagaacgtcc gatagagttc atatcatccg tcacttggat
     3601 taggaaacaa gataccaact ccccacgacg agcacgtcca gcattcaaga aggaaggagt
     3661 agcaggttgg tagcgttggt ggatgatttc attggcaata tcgattgcaa cagcttcatt
     3721 cccatcagcg aaataaaggg cgttgaagaa gacacggtct tccatatttt caagatagta
     3781 ttcaccgtca ttagtcttta aggcatattg attgtaaaat ttataagctg ccatgaatga
     3841 cttgaattgg aagttttggt ctttgataaa ttgagccaat tcttccaaga actctggacg
     3901 gtatttcttg ataaaggctg tttcgatgta gttgtgttca atgaggtaat tgattttgtc
     3961 tttgattgaa tcaaaaacca tagtgtttgg aactacattt tctttaaaga aagcatccaa
     4021 ggcttcctta tctttatgaa gcatgatttg tccattaaca ggacggttaa tttcgttatt
     4081 aagacggaag taagtcacgt cttcaagatg ttttaatccc ataaaatttc ccttatctaa
     4141 ttacaaaaga aaggcttcta agttagccct aaaagcagtt tcttctggat gatgtactaa
     4201 gattatgcta attgtttcag ttttcctggt tggaaacctg aaaagacttc agttggtgtt
     4261 tggataacag gagctgcgct aaaaccgagc tctttaactt gatcgacgta ctcaggttgc
     4321 tcatcaagat tgatttcacg ataagagaca ttattactgt ccaagaaacg cttggtcatt
     4381 ttacattgga cacaattgtt tttagaataa acggttacca ttgtgtaact cctcttcaaa
     4441 atttaatact atcttagtat atcagaaaat aaaattttgt caat
//
LOCUS       DAVKAO010000097         4452 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKAO010000097 DAVKAO010000000
VERSION     DAVKAO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.097"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(98..1084)
                     /locus_tag="VWX16_001901"
     CDS             complement(98..1084)
                     /locus_tag="VWX16_001901"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9159197.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALSFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1278..3746
                     /gene="gyrA"
                     /locus_tag="VWX16_001902"
     CDS             1278..3746
                     /gene="gyrA"
                     /locus_tag="VWX16_001902"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9159198.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQTQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLVITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3746..>4452
                     /gene="srtA"
                     /locus_tag="VWX16_001903"
     CDS             3746..>4452
                     /gene="srtA"
                     /locus_tag="VWX16_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9159199.1"
                     /translation="MSQKKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIV
                     WNTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIP
                     ELEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDN
                     AKNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERII
                     VKGDLKETKDYSQTSNE"
BASE COUNT         1353 a          791 c         1008 g         1300 t
ORIGIN      
        1 taatggggtg ctatttcaac ttcaacctac tataatacag aaaaaaatac agaaaaaaac
       61 aactccctga tgattcaagg agttgtctat agttaaatta gtttttagaa gcttcttgga
      121 attctggatt tttccatgct tcgtcaataa tagcttgcaa ttctttagca gatgcttgca
      181 ttttttgagt ttctgcatcg ttcaatggaa tgtttactgg acgaacgata ccatgtgcac
      241 caacaacagc tggttgaccg ataaagacat tctcaactcc gtattgacct tcttggaata
      301 ctgaaagtgg aagtactgcg ttttcatcgt caaggattgc tttagtgata cgagcaaggg
      361 ctactgcgat accgtagtat gttgcacctt ttttgttgat gattgtgtag gctgcatcac
      421 gaacaccttc gaacaattca atcaattcag cttcttgaac attttgagtg tctttaagga
      481 attcttcaag gtttacacca gcgatgttag cgtgtgacca aacagcgaac tcagagtcac
      541 cgtgttcacc catgatgtag gcgtgcactg aacgagcatc cacatccaat ttttcagcaa
      601 gtgcttgacg gaaacgagct gagtcaagtg aagtacctga accgataacg cgttctttag
      661 ggaaaccaga gaatttccaa gttgagtaag tcaaaacgtc aactgggtta gcagcaacaa
      721 ggaagatacc tttgaaacca gattcaacaa cttgagttac gattgatttg ttgatagcaa
      781 ggtttttacc tacaaggtca agacgagttt cacctggttt ttgaggtgca cctgcagtga
      841 tcacaacaag gtcagcgtct gcacagtcag agtattgagc tgcatagatt tttttaggtg
      901 aagtgaagga aagggcgtga ctaaggtcaa gcgcatcacc aacagctttt tcatgcaatt
      961 gtggaatttc gataattcca agctcttgtg caattccttg gttaacaagt gcaaaagcgt
     1021 aagatgaacc tacagcacca tcaccgacaa ggataacttt tttgtgttgt ttagttgaag
     1081 tcattgtttt aaacatctcc ttaattttat taggggattt tccctagaca acttcattct
     1141 atcactttta aaaaactttg tcacgaatat gccttatagt tctctatgta aacgttttag
     1201 tggtttagag gctgaaatag atgggaattt atggtataat gttgttactt actaattgtg
     1261 aaatgaggca tttattaatg caggataaaa atttagtgaa tgtcaatctg acaaaggaga
     1321 tgaaggcaag ttttatcgac tacgccatga gtgttatcgt agcgcgagct cttcctgatg
     1381 ttcgagatgg cttaaaacct gttcaccgtc gcattctcta cggaatgaat gaattgggtg
     1441 tgaccccaga caaaccccat aaaaaatctg ctcgtattac aggggatgtc atgggtaaat
     1501 atcacccaca cggggattcc tctatttatg aagccatggt ccgtatggct caatggtgga
     1561 gctaccgtta catgcttgta gatggtcatg ggaattttgg ttccatggat ggagatagtg
     1621 ctgccgctca acgttatacc gaggcacgta tgagcaagat tgctctggaa atgcttcgtg
     1681 atatcaacaa aaatacagtt gatttcgttg ataactatga tgccaatgaa cgggaaccct
     1741 tggtcttgcc agcgcgtttt ccaaaccttt tggttaatgg agcaactggt atcgcggttg
     1801 ggatggcaac caatattcca cctcataatc tgggtgaaac cattgatgca gtgaagttgg
     1861 tcatggataa tcctgaagtg actaccaagg acttgatgga agtcttgcct ggaccagatt
     1921 ttccaactgg tgctcttgtc atggggaaat caggtatcca taaggcttat gaaacaggta
     1981 aaggttcgat tgtcctacgt tctcgtacag agattgaaac gactaagact ggtcgtgagc
     2041 gcatcgttgt aacagaattt ccttacatgg tcaataaaac caaggtgcat gagcatattg
     2101 ttcgcttggt tcaggaaaaa cgcattgagg gtatcacagc agtacgtgat gagtcaaacc
     2161 gtgaaggtgt tcgatttgtt attgaagtca agcgcgacgc ctcagccaat gttattctca
     2221 ataacctctt caaaatgacc caaatgcaaa ccaattttgg tttcaatatg ctcgctatcc
     2281 aaaatggtat accgaaaatt ttgtctcttc gtcagatttt ggatgcttat atcgagcacc
     2341 aaaaagaagt ggttgttcgt cgtacacgtt ttgataagga aaaagcggaa gcgcgcgctc
     2401 atatcttaga aggtctcttg attgcgctag accatatcga cgaagtgatt cgtatcatcc
     2461 gtgctagtga aacggatgcg gaagctcaag ctgagttgat gagcaagttt aagctttctg
     2521 aacgtcaaac tcaagctatc cttgatatgc gtcttcgtcg tttgacaggt ttggaacgcg
     2581 ataagattca atctgagtat gatgacctct tggctctgat tgcggattta gcagatattc
     2641 ttgctaagcc tgaacgtgtt tctcaaatta tcaaagacga attggatgaa gttaaacgta
     2701 aattttctga taaacgccgt acagagttga tggttggaca ggtcttgagt ctcgaggatg
     2761 aggacttgat tgaagaatcg gatgtcttga ttaccctttc taacagaggc tacattaagc
     2821 gtttggatca ggacgagttc actgctcaaa aacgtggggg tcgtggtgtc caaggaacgg
     2881 gagtgaaaga tgatgacttt gttcgtgagt tagtgtcaac tagcacccat gatcatctgc
     2941 tcttcttcac aaacaaggga cgtgtctatc gtcttaaagg ttatgaaatt cctgagtatg
     3001 gtcggactgc caaagggcta ccagtagtca atctcttgaa attggatgaa gacgaaagta
     3061 ttcagacggt tatcaatgtt gagtctgatc gcagtgatga tgcttatctc ttctttacaa
     3121 cccgtcacgg tattgtgaag agaaccagtg ttaaggagtt tgccaatatt cgtcaaaatg
     3181 gtctcaaagc gctgaattta aaggatgaag atgagttaat caatgtcttg ttggcagaag
     3241 gagatatgga tattatcatt ggtaccaagt ttggttatgc agttcgcttt aatcaatcag
     3301 ccgttcgtgg tatgagccgt atcgccactg gtgtgaaagg tgttaacctt cgtgaaggag
     3361 acacagttgt tggtgccagc ttgattactg atcaagatga ggttcttgtt atcacagaaa
     3421 aaggatatgg taagcgtaca gtcgctactg aatacccaac aaaaggtcgt ggtggtaagg
     3481 gaatgcagac agctaaaatt accgaaaaaa atggcttgct ggccggtctt atgactgttc
     3541 aaggggatga ggatttgatg attatcactg atacaggtgt catgattcga accaatcttg
     3601 ccaatatttc acaaacagga cgtgcaacta tgggagttaa agtaatgcgc ctggatcaag
     3661 atgctcagat agtgactttc acaacggttg cggtggcaga aaaagaagaa gttgggacag
     3721 aaaacgaaac agaaggtgaa gcataatgtc tcaaaaaaaa aataataaaa agaaaaacaa
     3781 gcgaaaaaat ttgctgacaa atatcctagc aggatttctg atattactgt cactggcttt
     3841 gatttttaat actcaaattc gaaatatttt catagtctgg aataccaata agtatcaagt
     3901 tagccaggta tcaaaagaaa aattagaaga aaatcaggat acagaaggca attttgactt
     3961 tgattctgtc aaagctatct cttcggaagc tgttctaact tctcaatggg atgctcaaaa
     4021 attaccagtt attgggggaa ttgcaattcc tgaattggaa atgaatttgc cgatttttaa
     4081 aggacttgat aatgttaatc tcttctacgg agctggtaca atgaaacgcg agcaagtaat
     4141 gggagaagga aattatagtc tagctagtca ccatatcttt ggtgttgata atgctaataa
     4201 aatgttattt tctcctttag ataatgctaa aaatggcatg aagatttatc taaccgataa
     4261 aaataaagtt tatacttatg aaatacgtga agtcaaacgt gtgacaccgg atcgtgttga
     4321 tgaagttgat gatagagatg gggtcaatga aatcacatta gtaacctgtg aagaccttgc
     4381 tgctacagaa cgtattattg tcaaaggtga tttgaaagaa acaaaagatt attcacaaac
     4441 atctaatgaa at
//
LOCUS       DAVKAO010000098         4350 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKAO010000098 DAVKAO010000000
VERSION     DAVKAO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.098"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..1021
                     /locus_tag="VWX16_001904"
     CDS             <1..1021
                     /locus_tag="VWX16_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9159200.1"
                     /translation="NKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAF
                     GQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELN
                     GKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLW
                     RDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTS
                     KFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGD
                     DKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNA
                     CKNAK"
     gene            1011..2018
                     /locus_tag="VWX16_001905"
     CDS             1011..2018
                     /locus_tag="VWX16_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9159201.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2018..3025
                     /locus_tag="VWX16_001906"
     CDS             2018..3025
                     /locus_tag="VWX16_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9159202.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3025..3819
                     /locus_tag="VWX16_001907"
     CDS             3025..3819
                     /locus_tag="VWX16_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9159203.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHINS"
BASE COUNT         1577 a          636 c          736 g         1401 t
ORIGIN      
        1 aaacaaattt tttctaatag ctattttagc tatgtgtata gtttttagcg cttgttcttc
       61 taattctgtt aaaaatgaag aaaatacttc taaagagcat gcgcctgata aaatagtttt
      121 agatcatgct ttcggtcaaa ctatattaga taaaaaacct gaaagagttg caactattgc
      181 ttggggaaat catgatgtag cattagcttt aggaatagtt cctgttggat tttcaaaagc
      241 aaattacggt gtaagtgctg ataaaggagt tttaccatgg acagaagaaa aaatcaaaga
      301 actaaatggt aaagctaacc tatttgacga tttggatgga cttaactttg aagcaatatc
      361 aaattctaaa ccagatgtta tcttagcagg ttattctggt ataactaaag aagattatga
      421 cactctatca aaaattgctc ctgtagcagc atacaaatct aaaccttggc aaactttatg
      481 gagagatatg attaaaattg attcaaaagc cttaggtatg gaaaaagaag gtgatgagtt
      541 aatcaaaaat actgaagctc gtatatccaa agaattagaa aaacatccag aaatcaaagg
      601 aaaaatcaaa ggaaaaaaag tattatttac tatgattaat gctgcagata catcaaaatt
      661 ctggatttat actagcaaag atccaagagc aaattattta acagatttag gtctagtttt
      721 ccctgaatca ttaaaagaat ttgagagtga agatagtttt gcaaaggaaa tttctgcaga
      781 agaagcaaat aagataaatg atgctgatgt aatcataact tatggtgatg ataaaactct
      841 tgaagcttta caaaaagatc ctcttttagg taaaataaat gcaattaaaa atggtgccgt
      901 tgctgtaatt ccagataata caccgttagc agcctcatgc actccaacac cactttcaat
      961 aaactatact attgaagaat acctaaatct tttaggaaat gcatgcaaaa atgcgaaata
     1021 aaaaacaaat aaacctaggc ataatttttg taatctgcct aggtcttctt attacaatat
     1081 ttttgtcatt aaagcttgga acaaaagaaa ttaatatcag agatttttta gcagcttttg
     1141 gaatgggcaa tacaaatgat gattttatta aatcaattat atataaaaga atacctagaa
     1201 ctatttttgc aattttagca ggttctagtc ttgccataag cggtgtattg atgcaatcag
     1261 ttactagaaa cccaatagct gatccaggta tactcggtat aaacacagga gcaagtctta
     1321 gtgtagtaat tggtctttct tttttaggaa tttcatcaag cataagccat ataagttttg
     1381 caatcattgg tggcttagta agtgcaattt ttgtatacgc gattgctgta agcggaaaag
     1441 caggccttac ccctataaaa cttgccttat caggaacttg tgttagtatg gctttaagca
     1501 gttttgtaag ttttttaatt ttaccgaata ataacgtctt agacaaattt agattttggc
     1561 aaataggtag ccttggagca gctacattat cttctatatc tacactacta ccttttataa
     1621 ttttaggtca cttgatagct atatttattt catcagattt aaacgcttta gctatgggtg
     1681 atgaaatggc tgttggtctt ggagttaatg ttaataggat aagatcactt gcaataattg
     1741 caagtgtgct tttatgttca agtattactg caattggtgg acctattggc ttcgtaggtc
     1801 ttatagttcc tcacttttgt ggcttattta taagcaaaga tatacgcaca atgaccattt
     1861 cttcatcttt tataggtgca gagctcttgc ttatatgtga tataatcggc cgtatgttag
     1921 gtaaaccagg tgaaattgaa gtagggataa ttactgcaat aatcgggggt ccagtactta
     1981 tttatgtaac tatgaaaaat agaggggtta ataactaatg caaaatttaa ttataggtat
     2041 tcaaaaaaga aaaaatagaa taacactatt ttcctcacta tttttattaa taataatcag
     2101 tctatcattt ttcattttac ttatcggaga tgaaagttat tctttttcaa ctttgattaa
     2161 agtcttaaat agtgaaactg ttcctggagc tagtttttcg attatggaaa ttagattacc
     2221 aaaattatta gcaggaatta tagctggctg gtcttttgga ttggcaggat ttatctttca
     2281 aactatgtta agaaatcctc ttgcaagtcc tgatataatc ggtgtcacaa gttcttcatc
     2341 tattgcagcg gtcttttgca tattggtatt aaaaacaaat agtttaacta ctggaattat
     2401 ttcaataact tgtggactaa catcatcttt aatattattt ttactagcta aaaaagatgg
     2461 tttttcagca gcaagactga taatattagg tattggtttt caagctgtca caagagcagg
     2521 cacctcattt ttattgttga aagtagcaag atatgaatta caagaagtta tgagatggct
     2581 cagtggctct ttatctttta caaagttaga tgacatacct cttgttctaa tagtaagtat
     2641 tattgctact atattagttt tattttttaa taaaagacta gaaattattg aacttggtga
     2701 agaaatagca atcggacttg gagcaaatcc cgagctttca aggcttgttt taattttttg
     2761 cgctgtatct ttaactgctt tttctacttc aattacagga ccaatagctt gtatatcttt
     2821 tttagctggt cccatagcct taaatattgg caagaaaaga agcccaatat tagctggatt
     2881 ggttggaatt ttactagttt tgttatcaga catattctct caaaatattt taccagctag
     2941 atatccagta ggtgttgtaa ctggcttgtt aggttcacca tacttaatat acttactaat
     3001 aaaaatgaac aggaggaata tataatgaaa ggtctgtggt caaataatct tacctgcggt
     3061 tatgatgaaa aaataatctt ggaaaatata aatataaaaa tacctgaaga aaaaatatca
     3121 gttattattg ggtcaaatgg ttgtgggaaa tcaacactca ttaaaacctt gtctcgactt
     3181 ataaagccat tagagggaga agtattgctt gataataaat caattaattc ttataaagaa
     3241 aaagatttag caaaacacat agctatatta cctcaatctc caataatccc tgaatcaata
     3301 acagtagctg atcttgtaag ccgtggtcgt ttcccctaca gaaagccttt taagagtctt
     3361 ggaaaagatg accttgaaat aataaacaga tcaatggtta aggccaatgt tgaagatcta
     3421 gcaaataacc tagttgaaga actttctggg ggtcaaaggc aaagagtatg gatagctcta
     3481 gccctagccc aagatacaag tatcctactt ttagatgagc caactactta cttggatatc
     3541 tcatatcaaa tagaactatt agacctcttg actgatctaa accaaaaata taagacaacc
     3601 atttgcatga ttttgcacga tataaatcta acagcaagat acgctgatta cctatttgca
     3661 attaaagaag gtaaacttgt tgcagaggga aagcctgaag atatactaaa tgataaacta
     3721 gttaaagata tctttaatct tgaagcaaaa attatacgtg accctatttc caattcgcct
     3781 ctaatgattc ctattggcaa gcaccatatt aactcttaat aattgaatat tggaattcat
     3841 ttaaaagtcg caaattttga actgaccctc aaaagttagg ccaaaaaact aactaaagga
     3901 ggtcagtttt ttaatggcaa atacagcaca gaatttaaga taaaattagt aaaagaatat
     3961 catgaatgaa aaataggata tagagaataa cagcaatatt aaaaagatca ggactgataa
     4021 taaacaaaaa gaaagtacaa cgactagttc aaaagctaaa acttcaagta aaaagttatt
     4081 caagaaaatc tagaaaatac tcatcctaca aaggacaggt aggaaaaata tcagacaaca
     4141 aaataaaaag aaatatttat taccgaaaaa aaggggaaat cgagtcaagt accaacaggc
     4201 tcatctattc caaaaagata tttactcgat ggaaaaactc ctagaattag tgctacaaat
     4261 attgacaatg gtatcttagg ttactacgaa gatatagatg ataaaaatta tagagttttt
     4321 gaaaatttta tatctgtttc atttttagga
//
LOCUS       DAVKAO010000099         4295 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKAO010000099 DAVKAO010000000
VERSION     DAVKAO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.099"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(52..468)
                     /locus_tag="VWX16_001908"
     CDS             complement(52..468)
                     /locus_tag="VWX16_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9159204.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEQTERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(480..936)
                     /locus_tag="VWX16_001909"
                     /pseudo
     CDS             complement(480..936)
                     /locus_tag="VWX16_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1036..1248)
                     /locus_tag="VWX16_001910"
     CDS             complement(1036..1248)
                     /locus_tag="VWX16_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9159205.1"
                     /translation="MIGESMEGYTPGELTSIELLEGRYARIEALSVEKHAEDLLAVYG
                     PDTPQEMWTYLFQEPVADMEELVSLL"
     gene            complement(1466..3967)
                     /locus_tag="VWX16_001911"
     CDS             complement(1466..3967)
                     /locus_tag="VWX16_001911"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9159206.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDISREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
BASE COUNT         1194 a          989 c          813 g         1299 t
ORIGIN      
        1 tatataatac tagaccatcc ttaaattttg cttaagaaaa aacaagtttt gttatttttt
       61 acgattcatc caagtcatcc ctatacaatt ataggtggat aagatttcaa agcccataga
      121 acgatagaag cccaaggttc tttctgtctg ttctgtcacc agctggactt gataggcatc
      181 tttgtaatcc tctaaagcct ctttcatcaa ggcactacca attccttgac gctgatagat
      241 tggtaaaacg attaaatcct gaaccaatac tgatgagaat ccatctccaa ccaaacgaat
      301 caagcccacc acagcatcac catcaagtgc cagataaatt actaatgaat gagataaggc
      361 ctgctccagc atctctggtt gatgggtata atttgtccaa ccgacagcct gatagagatg
      421 taaaacatcc tctagcttga caatttcttg ctttttaata gtaatcatct caacacctct
      481 taaagttctc tcaagctctt gtactgctgt ccatttttat caaaattttc aggacgcaac
      541 catgtttcca aacgatcttt gactttgggc cagtccttat caatcataga caaccaatcc
      601 atatctctcg tacgcccctt ataaaccact gcctgacgga aggttccttc ataaataaag
      661 cccaaacgct ccgcagcacg tctggatggc aggttaagag catcgcattt ccactcataa
      721 cgacgatagt taagctcttc aaagacatag cgagccagta gatactgggc ttctgtccct
      781 atccgtgtcc ccctgagttt tggagaaaaa gtgacagctc ccacttctat tactccgtta
      841 ctggtcaata cgcatgagag aaaaagttcc caaagcctta ccagttgcct tgtctatgta
      901 gtaaaaacgg tccttacgag ccaacatctg atttatagta aattgaaact aaaatagtac
      961 acttatactt ctaaaacatt gttagaaatc gatttgactg tcctgatcga tttgtcctgt
     1021 tcttatttca ttttactata aaaggctgac cagctcctcc atgtctgcta ctggctcctg
     1081 aaagaggtag gtccacatct cctgaggcgt atcaggacca taaacagcta gcaaatcctc
     1141 cgcatgcttt tccactgaaa gagcctctat cctagcataa cgcccttcta agagttcaat
     1201 agaagtcagc tcacctggtg tataaccttc cattgactcc ccaatcattt gaccatattc
     1261 atttactggc atagctttct ccttttttca cttttcaaac tttatagttg cccatagtat
     1321 accatacttt cataagaaga ttcaaaaaat cctccagatt ctactctgaa ggattcctac
     1381 cttatttcac aacgatatta acgagtttag ttggctcaaa cagtttggga aactgtttga
     1441 ggttggaaat cagcaaataa attggttatt ttacaacaat attaaccaat ttatttggta
     1501 cactaattac tttcacgatt tccttaccgt caatttctgc tttgactttt tcatcagcta
     1561 aagcgatttc ttgtaattct tcacgtgata tatctttagc aaccatgagt ttggcacgga
     1621 ctttaccttt gatttggaca acgatttcga tttcgtcttc aaccagttta ctttcgtccc
     1681 aagttggcca agctacataa gagattgact cacctgtttc tgcgactgtt tgccagagtt
     1741 cttctgccaa gtgaggtgca aatggtgcaa tcaattgaat aaagcctttg gcatagtcaa
     1801 cataaagctt atcttccttg ttagcagcat tgacaaagac cataagttgg gcaatagctg
     1861 tgttgaattt gagagactca atttgctcag taacagcttt gactgtttcg ttgtaagcct
     1921 tgtcaagagc accattgttt tccgcaagga tttctttact tgtaatcaaa cggtaaactc
     1981 ggtcaaggaa cttacggctt ccttccaaac cttcttctga ccaagcaatc gaagcatcga
     2041 gtggtcccat aaacatttca taaacacgaa gggtatcggc accgtattgt tccaccacat
     2101 catctgggtt aacaacgttc ttgagcgatt tagacatctt ggctggcgct tgctccaact
     2161 cttcccctgt ttctatatga aagaaggaac catcacgttt ttcaaccttg tcggttgcca
     2221 caagagcacc acggtggtca cggtagcttg ttcccaaaat catcccttgg ttaaagagtt
     2281 tttggaatgg ttccttagtc ggaacaacac cgaggtcata gaggaattta tgccagaaac
     2341 gagcataaag caagtgaagt acagcatgtt ccgcaccacc cacgtagata tctactggca
     2401 accattgttt gaggaggtcc tcatcagcca atttctcagt attgtgcggg tcaatatagc
     2461 ggaggtagta ccagcttgaa ccagcccatt gtggcatggt gttagtttca cgacgacctt
     2521 tgacaccatc ttcacgagtc acttcaagcc aatctgtcaa gttagctagt ggactttcac
     2581 cagtacctga aggacggata tccttggtta caggcaagac aagcggcaat tcagtttcag
     2641 gaacagctgt tgaagttcca tcttcccaat gaatgattgg aattggctca ccccagtaac
     2701 gttgacggct aaagagccag tcgcggaggc ggtaggtaac cttctcctga ccacagcctt
     2761 tttcttccaa gcaagccaca atcttggcaa tagcgtcttc tttgttcaat ccatctagga
     2821 agtctgaatt gacatgcagg ccatcctctg tgtaggcagc ttcttcgaca tttccacctt
     2881 caagtacttc gacgattgga aggtcaaatt gtttggcaaa ttcccagtca cgttggtcgt
     2941 gggcaggcac agccataacc gcacctgttc cataactagc aagaacatag tccgcaatcc
     3001 agattggcat ttccttacca ttgacagggt tgatggcata ggcacctgtc caaacaccag
     3061 ttttttcttt agcaaggtct gtacgagcca agtcagactt aaggctggct tggtgtttat
     3121 agtctgctac agcttctgct tgctctgaac ttgtgatagc gtctactaat tcatgttcag
     3181 gagccaagac agtgaaagtc gcaccgaaaa gtgtgtccgg acgagtagta aagactgtaa
     3241 attccttgtc tgttcctttt actttgaaag ttacattggc accagttgat ttaccaatcc
     3301 agttgcgttg catatccttg atagactctg accaatctag ttcatctaag tcattgagca
     3361 agcgctctgc gtaagccgta attttgagca tccattggcg cattggtttg cggacaactg
     3421 gatagcctcc acgctcagaa gttccgtcag gaagcacttc ttcattggca atggcagttc
     3481 ccaattcctc aacccagttt actggcactt cagcttcata ggccaagcct ttttcgtaaa
     3541 gcttggtgaa aatccattga gtccacttgt agtagtttgg atctgttgtg ttgacttcac
     3601 gatcccagtc ataagaaaat ccaagcgcat tgatttgacg tttgaagttg gcaatgtttt
     3661 ccgctgtaaa ttctgctggg tcattaccag tatccatagc gtattgctct gcaggcaaac
     3721 caaaagcatc ccaacccatt gggtgaagaa cattgtagcc ttgcgcacgt ttgtaacgac
     3781 tgaggatatc ggttgcagta taaccttctg ggtgtcctac gtgcagacca gctccagacg
     3841 gataagggaa catatcaagc gcataaaact taggttttga tgcatctgtt cctgtcttaa
     3901 atgtatgatg ttctgcccag tagccctgcc acttaggctc aatttcttta tgattgtaaa
     3961 aactcatggt ctctctccaa ttttgtgatg ttactattat accatttttg agggaatttg
     4021 aaatgtgttt aaaggaaaaa ctgtatactg tatctcatta attttgatga ctatgaattg
     4081 ctctatctcc cactttttat cccattgtta tgcagtaaat caaggagtaa gtcaagaagc
     4141 ttatctcgta tagcacaaaa tacttatttt agaccgaaga taaaactgga atgctaatta
     4201 atgtattcac aataaatagc cgactaccta tgtcgctttt tgatttatga agaacgtata
     4261 gtagactgaa tctgaaatag tacgaaacaa ttgct
//
LOCUS       DAVKAO010000100         4277 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKAO010000100 DAVKAO010000000
VERSION     DAVKAO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.100"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            9..326
                     /locus_tag="VWX16_001912"
     CDS             9..326
                     /locus_tag="VWX16_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9159207.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            326..2017
                     /locus_tag="VWX16_001913"
     CDS             326..2017
                     /locus_tag="VWX16_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9159208.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            2232..3638
                     /gene="lacG"
                     /locus_tag="VWX16_001914"
     CDS             2232..3638
                     /gene="lacG"
                     /locus_tag="VWX16_001914"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9159209.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVNNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            3681..3938
                     /locus_tag="VWX16_001915"
     CDS             3681..3938
                     /locus_tag="VWX16_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9159210.1"
                     /translation="MFKDFIQSIYEKVYIINFEKYSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDDLEEFKNLFLNFINPEEWDTISFDSDFMPFQQS"
BASE COUNT         1287 a          791 c          859 g         1340 t
ORIGIN      
        1 attctatcat gaatagagaa gaagtaacat tgttaggttt tgaaatcgta gcctatgctg
       61 gcgatgctcg ttcaaaacta ttggaagcct tgaaggctgc tgaagctggt gattttgaaa
      121 aagcggacgc tctggtagag gaagctggta gctgtattgc agaggctcac cacgcgcaaa
      181 caagtctatt gactaaggaa gcttcaggtg aggacttggc ttatagtgta accatgatgc
      241 atggccaaga ccacttaatg acaactatct tgttaaaaga tttgatgcat catttaattg
      301 aactctacaa gagaggagtt aaataatgaa taaactaatt gcatttatcg agaaaggaaa
      361 gcctttcttt gaaaaactat ctcgtaatat ctatcttcgt gctattcgtg atggtttcat
      421 tgcaggtatg cctgttattc tcttctcaag tatctttatc ttgattgcct ttgtaccaaa
      481 ctcatggggc tttaaatggt ctgatgaagt tgtagccttt ctgatgaaac cttatagcta
      541 ttctatgggt attctggctc tcttggtagc tggtacaaca gctaagtcat tgactgactc
      601 agtaaaccgg agcatggaaa aaaccaatca aatcaactat atgtcaacat tgttggcagc
      661 aattgttggt ttgttgatgt tggcagctga tcctatcgaa agtggtctag ctactggatt
      721 cttggggaca aaaggtttgc tttcagcctt ccttgctgcc ttcgttactg tagccatcta
      781 taaggtttgt gttaagaaca acgtcactat tcgtatgcct gacgaagttc caccaaatat
      841 ctcacaagtc tttaaagatg tgattccatt cactctctca gttgtttcac tttatgctct
      901 tgatttacta gctcgtcatt ttgttggtgc aagcgtagct gaatcaatcg gtaaattctt
      961 tgcaccactc ttctcagcag cagacggata ccttggtatt accattatct ttggtgcctt
     1021 tgccttcttc tggtttgtgg gtattcatgg tccatctatt gttgaacctg ctatcgcagc
     1081 aattacctat gcaaatgctg aagtcaactt gaaccttctc caacaaggta tgcacgctga
     1141 caagattctt acttctggta cacaaatgtt tatcgttacc atgggtggta ctggtgcgac
     1201 actggttgtt ccattcatgt tcatgtggtt aacaaaatca aaacgtaacc gtgcaatcgg
     1261 acgtgcttca gtagttccta ccttcttcgg tgtaaatgag ccaatcttgt ttggtgcacc
     1321 tcttgttttg aatccaatct tcttcattcc atttatcttt gctccaattg caaacgtatg
     1381 gatctttaaa ttctttattg aaactcttgg aatgaactca ttcactgcta atctaccatg
     1441 gacaacccca gctccactag gtctagttct tggtactaac ttccaagttc tatcattcat
     1501 tcttgctgcc cttctaatcg tggttgacgt tgtcatttac tatccattcc ttaaggtcta
     1561 tgatgaacaa attcttgaag aagaacgttc aggtaagtct aatgatgaat tgaaagaaaa
     1621 agttgctgca aacttcaaca ctgcaaaagc ggatgctatt cttgaaaaag caggtgtaga
     1681 agcagcacaa aatacaatca ctaaagaaac aaatgtcctc gttctttgtg caggtggagg
     1741 aacaagtggt ctccttgcaa atgctctaaa caaggcagca gcagaataca atgtccctgt
     1801 gaaagcagca gcaggtggct atggtgctca ccgtgaaatg ttgccagagt ttgatttggt
     1861 tatccttgct cctcaagttg cttcaaactt tgaagatatg aaggcagaaa cagataagct
     1921 cggtattaaa cttgctaaga cagaaggcgc tcaatacatc aaattaactc gtgatggaaa
     1981 aggtgcccta gcattcgtac aagagcaatt cgattaaggc taggaactgt gaaacagtct
     2041 cccatcgtta cggaaatcgc tatggcgaat ttcctaatcg ccttattaat tcgtcggtaa
     2101 aaagatatcg tttttacctc ctcatgtcac aattcggtga cttggtacaa gaagtgagat
     2161 ggagaaggat ggctcactga ctcctctcct ctcactttta ctttatttaa atcaagaaat
     2221 aggtgaaaaa aatgacaaaa acacttccaa aagactttat ttttggtggc gcaacagctg
     2281 cctatcaagc agaaggtgct acacatactg atggtaaagg accagtagcg tgggataaat
     2341 atcttgaaga taactactgg tacactgcag aaccagccag tgatttttac aatcgatatc
     2401 cagttgacct caagctagca gaagagtatg gtgtcaatgg cattcgaatt tctattgcct
     2461 ggtcacgtat tttcccgact ggttatggtc aagtaaatgc taaaggtgtt gagttttatc
     2521 ataatttatt tgcagagtgt cacaaacgtc atgttgagcc ctttgtaact cttcatcact
     2581 ttgacacgcc agaagctctc cactcaaatg gagacttctt aaaccgtgaa aatatcgaac
     2641 attttgtaga ctacgctgcc ttctgttttg aagaatttcc agaagtaaac tattggacaa
     2701 cctttaatga aattggacca atcggtgatg gtcaatattt ggttgggaaa ttccctccag
     2761 gtatccagta cgaccttgcc aaagtctttc aatcacacca caatatgatg gtgtctcatg
     2821 catgcgcggt aaaattgtac aaagagaaag gctataaagg ggaaattggt gttgttcacg
     2881 ccctgcctac taaatatcct ctagatcctg aaaatccagc agatgttcgt gcagcagagt
     2941 tggaagatat catccacaat aaattcatct tagacgcaac ttatctaggt cgctattcag
     3001 ctgaaaccat ggaaggtgtc aaccatatct tatcagtcaa tggtggtagt ttggatcttc
     3061 gtgaagaaga ttttacagca ttagaagctg caaaagactt gaatgatttc ctaggaatca
     3121 actactatat gagtgactgg atggaagcct ttgatggaga aactgaaatt atccataatg
     3181 gtaaaggtga aaaaggaagc tctaagtatc aaatcaaagg tattggtcgt cgtgtagctc
     3241 ctgactatgt atcacgcacg gattgggatt ggattatcta ccctcaaggt ttgtatgacc
     3301 aaatcatgcg tgtgaagaaa gattatccta actacaagaa aatttatatc actgaaaatg
     3361 gtctcggcta taaagatgag ttcgttaata acactgttta cgatgatggt cgtattgatt
     3421 acgtgaaaca acacttggag atcttgtctg atgcgattgc agatggagct aatgtaaaag
     3481 gttacttcat ttggtcatta atggatgtct tctcatggtc aaacggttat gagaaacgtt
     3541 atggtctctt ctacgtagat tttgaaactc aagaacgtta tcctaagaaa tcagctcact
     3601 ggtacaagaa agtagcggaa actcagatta tagactagta gaattagtca ttagatatag
     3661 aattttagtg agtcaaaaag atgttcaaag attttatcca atctatttat gaaaaagttt
     3721 atattataaa tttcgaaaaa tactctcaaa taccgtgttt gacgagtgaa gaattgaaaa
     3781 gtcttggaaa atggtatgtc tcgactggta aagaatggat ttgtcattca gatgatgatc
     3841 tggaagaatt taaaaatcta tttttaaatt ttatcaatcc tgaagaatgg gatactatct
     3901 cctttgattc agattttatg ccgtttcaac aatcgtaacc aatttctcaa aaaagttaaa
     3961 tcttatattt agtactctgt aaaactctta tctaatcacg ttgcttatac tcaatgaaaa
     4021 tcaaagagca actttaaact aggaagcgag tcgcagattt ctcaatgtat agctttgagg
     4081 aattgggcaa aaagtctttg atatagaaaa aagcatagta tcaggtgttt caacacctga
     4141 tactatgctt tttattgtgg gaagatttac tttttttctt ctgaaattga gttgttaccc
     4201 aggctctttc agtttattaa ggcttgatga ctttaatgtg tttagatagc ttaaaaagga
     4261 ttgaatcact tagttta
//
LOCUS       DAVKAO010000101         4119 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKAO010000101 DAVKAO010000000
VERSION     DAVKAO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.101"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            619..1611
                     /locus_tag="VWX16_001916"
     CDS             619..1611
                     /locus_tag="VWX16_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9159211.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1613..2431
                     /locus_tag="VWX16_001917"
     CDS             1613..2431
                     /locus_tag="VWX16_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9159212.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2433..3218
                     /locus_tag="VWX16_001918"
     CDS             2433..3218
                     /locus_tag="VWX16_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9159213.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLIFFVISLKLWDKGLDSYESAGS"
     gene            3257..3535
                     /locus_tag="VWX16_001919"
     CDS             3257..3535
                     /locus_tag="VWX16_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9159214.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3472..3717
                     /locus_tag="VWX16_001920"
     CDS             3472..3717
                     /locus_tag="VWX16_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9159215.1"
                     /translation="MSRRKKAYQGRKIGSQLFATLESEARKKVGYLQVKTVAEGSNKD
                     YDRTNDFYRGFGFKKLEIFLQLWNPQNPCQILIKKLE"
BASE COUNT         1133 a          713 c          899 g         1374 t
ORIGIN      
        1 aatttatacg agatgatgaa agccttaagt gttattttac aaaggttatt tcaagtcgtt
       61 ccaaggtaac aagtctagat cagattgaag ctgataaaac gatacaaaga aaatattcaa
      121 gtgagctaaa aaaatttatt ggattttata atgagattat ttgtgaggaa aatagtttcc
      181 tacatgtacg aaagaggtgg tcgagttggt ttaggtagtc ggtgcgtgag ttgataattc
      241 tcagggtatg gacttctttt tcatgaatga ggtaaaagag caggtattgt ttagagacaa
      301 tcattctgag catattttct ggatagaggg agtatccgat tttatgatca aagttaatac
      361 cgccctctgg tgagaagatg agtaggttgg taatttaaac tattaaacag aatttttgat
      421 taaaagtatt atttcatgag agaaatccta atttcacaat ccataggcaa acgcttgcat
      481 tctgtttttt attggactat aataggttgg tataaagcct tctgtagtaa taaaatgtag
      541 aaggtgtaga aagtaaggat ttagaatatt tgtagttaaa aacacaatgt tgctattcct
      601 tatgataggg agatagatat ggcaatgata gaagtggaac atcttcagaa acattttgtg
      661 aagattgtta aggaaccggg cttgaagggg gctttgcgct cctttattca tcctgaaaag
      721 cagacctttg aagcggtcaa ggatttgacc tttgaggttc caaaagggca gattttagga
      781 tttatcgggg caaatggtgc tgggaagtcg acaaccatta aaatgctgac aggaattttg
      841 aaaccaacat ctggtttttg tcggattaac ggcaagattc cccaggacaa tcggcaagat
      901 tatgtcaaag atattggcgt agtctttgga caacgcaccc agctatggtg ggatttggct
      961 ctgcaagaga cctacactgt cttaaaagag atttatgatg tgccagactc gctctttcat
     1021 aagcgtatgg actttttgaa tgaagtcttg gatttgaagg actttatcaa ggatcccgtg
     1081 cggactcttt cactgggaca acggatgcgg gcggatattg cggcctcctt gctccacaat
     1141 cccaaggttc tttttttaga tgagccgacc attggtttgg acgtttcggt taaggataat
     1201 attcgtcggg caattactca gatcaatcaa gaggaagaaa ctaccattct tttgaccact
     1261 cacgatttga gtgatattga gcaactttgt gatcggattt tcatgattga caaggggcaa
     1321 gagatttttg atggaacagt gagccaactc aaggagacct ttggtaagat gaagactctc
     1381 tcttttgaac tgctaccagg tcaaagtcat ctcgtctctc actatgaagg tctgtctgat
     1441 atgaccattg atagacaagg aaacagcctc aacattgaat ttgatagttc tcgctaccag
     1501 tcagctgaca ttatcaagca aaccctgtct gattttgaaa tccgcgattt gaagatggtg
     1561 gatacggata ttgaggatat tatccgtcgc ttctaccgaa aggagctcta ggatgatcaa
     1621 attgtggaga cgttataaac cctttatcaa tgcaggggtt caggagttga ttacttaccg
     1681 agtcaacttt attctctatc ggattggcga tgtcatgggg gcttttgtgg ccttttatct
     1741 ctggaaggct gtctttgatt cttcgcaaga gtctttgatt cagggcttca gtatggcgga
     1801 tatcaccctc tacatcatca tgagttttgt gaccaatctt ctgactagat ccgattcgtc
     1861 ctttatgatt ggggaggagg tcaaggatgg ctccattatc atgcgtttgt tgcgaccagt
     1921 gcattttgcg gcctcctatc ttttcaccga gcttggttcc aagtggttga tttttatcag
     1981 cgttggcctt ccatttttaa gtgtcattgt cttgatgaaa atcatatcgg gtcaaggtat
     2041 tgtagaggtg ctaggattaa ctgtcattta tctttttagc ttaacgctcg cctatctgat
     2101 taactttttc tttaatattt gctttggatt ttcagccttt gtgtttaaaa atctttgggg
     2161 ttccaaccta cttaagactt ccatagtggc ttttatgtcg gggagtttga ttcccttggc
     2221 attctttcca aaggttgttt cagatattct ctcctttttg cctttttcat ccttgattta
     2281 tactccagtt atgatcattg ttggaaaata cgatgccagt cagattcttc aggcactcct
     2341 tttgcagttc ttctggctct tagtgatggt gggattgtct cagttgattt ggaaacgggt
     2401 ccagtccttt atcaccattc aaggaggtta gtatgaaaaa atatcaacga atgcatctga
     2461 tttttatcag acaatacatc aaacaaatca tggaatataa ggtggatttt gtggttggtg
     2521 tcttgggagt ctttctggct caaggcttga atctcttgtt tctcaatgtc atctttcaac
     2581 atattccatt cctagaaggc tggacctttc aagagatagc tttcatttat ggattttcct
     2641 tgattcccaa gggaatggac catctctttt ttgacaatct ctgggcacta gggcaacgcc
     2701 tagtccgaaa aggggagttt gacaagtatc tgactcgtcc catcaatcct ctctttcaca
     2761 tcctagttga aacctttcag attgatgcct tgggtgaact cttagtcggt ggtattttat
     2821 tgggaacaac agtgaccagc attgtttgga ctcttccaaa atttctgctt ttcctagttt
     2881 gtattccttt tgcgaccttg atttatactt ctcttaaaat cgcaacagcc agtatcgcct
     2941 tttggactaa gcagtcaggc gccatgattt acatcttcta tatgttcaat gactttgcta
     3001 agtatccgat ttctatttac aattctcttc ttcgttggtt gattagcttt atcgtgcctt
     3061 tcgcctttac agcctactat ccagctagct atttcttaca ggaaaaggat gtgttcttta
     3121 acgtaggagg tttgatgttg atttctctga ttttctttgt tatttccctt aaactttggg
     3181 ataagggctt agattcctac gaaagtgcgg gttcgtaaaa gctaaagtaa gactaaaatc
     3241 aagaaaggaa cttatgatgt ttgtaattga agaagtcaag gatgaaaatc aaaaaaaggc
     3301 agttgtcgct gaggttttga aggatttgcc agaatggttt ggaatcccag aaagcacaca
     3361 agcctatata gaaggaacca cgacactgca agtttggacc gcctatcagg agagtgattt
     3421 gactggattt gtaagcttat cctattcgag tgaagattgt gcagagattg attgtctcgg
     3481 cgtaaaaaag cttatcaagg tagaaaaatt gggagccaat tgtttgctac tttagagagt
     3541 gaagctcgta aaaaagttgg ttatctgcag gtcaaaacag tggcagaagg ttctaataaa
     3601 gattatgatc gaacaaatga cttttatcga ggttttggct ttaaaaagtt agagattttt
     3661 cttcaactat ggaatccgca aaatccttgt cagattttga ttaaaaagct tgaataatat
     3721 tatttgacat ctattctcag agtgctatac tgtaagtgta atcgccgatt tagctcagtt
     3781 ggtagagcaa ggcactcgta aagcctaggt tataggtaga taaacgactg aggagttgaa
     3841 aaaatagata ggtagaagat aaccgttaag ccttattctt agcggttatt tatattgttt
     3901 aatagcgctg atatttcatc aattatgcct gttttcgtgt ttctggtagt tgttcaagtt
     3961 tattgctact atttttgatg gtatgaatgt gcttataatg tatcccggtt aacgaaagtt
     4021 ttggacttat actcttagaa aatctcttca aaccacgtca acgtcgcctt gccgtgcgta
     4081 tggttactga cttcgtcagt tctatccaca acctcaaaa
//
LOCUS       DAVKAO010000102         3844 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKAO010000102 DAVKAO010000000
VERSION     DAVKAO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.102"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..444
                     /locus_tag="VWX16_001921"
     CDS             <1..444
                     /locus_tag="VWX16_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9159216.1"
                     /translation="FGNASDHVRKNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAG
                     LNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSI
                     RQNAMETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            654..3773
                     /gene="phtE"
                     /locus_tag="VWX16_001922"
     CDS             654..3773
                     /gene="phtE"
                     /locus_tag="VWX16_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9159217.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKEADIVNEVKGGYIIKIDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEEVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQQSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1436 a          626 c          756 g         1026 t
ORIGIN      
        1 tttggtaacg ctagcgacca tgttcgtaaa aataaggcag accaagatag taaacctgat
       61 gaagataagg gacatgatga agtaagtgag ccaactcacc ctgaatctga tgaaaaagag
      121 aatcacgctg gtttaaatcc ttcagcagat aatctttata aaccaagcac tgatacggaa
      181 gagacagagg aagaagctga agatactaca gatgaggctg aaattcctca agtagagcat
      241 tctgttatta acgctaagat agcagatgcg gaggccttgc tagaaaaagt aacagatcct
      301 agtattagac aaaatgctat ggagacattg actggtctaa aaagtagtct tcttctcgga
      361 acgaaagata ataacactat ttcagcagaa gtagatagtc tcttggcttt gttaaaaaaa
      421 agtcaaccgg ctccgataca gtagtaaaat gaatggagca tattttatgg agaagtaacc
      481 tttcgtgtta cttctctttt ttagaaaaat gtaacagaaa cttgacaaaa gtaattttaa
      541 atagtaaact atttactggt taattaaatg gttaaataac cggtttagaa aactatttaa
      601 taaagtaaaa gaagttgaga aaaaacttca tcatttattg aaatgaggga tttatgaaat
      661 ttagtaaaaa atatatagca gctggatcag ctgttatcgt atccttgagt ctatgtgcct
      721 atgcactaaa tcagcatcgt tcgcaggaaa ataaggacaa taatcgtgtc tcttatgtgg
      781 atggcagcca gtcaagtcag aaaagtgaaa acttgacacc agaccaggtt agccagaaag
      841 aaggaattca ggctgagcaa attgtaatca aaattacaga tcagggctat gtaacgtcac
      901 acggtgacca ctatcattac tataatggga aagttcctta tgatgccctc tttagtgaag
      961 aactcttgat gaaggatcca aactatcaac ttaaagaggc tgatattgtc aatgaagtca
     1021 agggtggtta tatcatcaag atcgatggaa aatattatgt ctacctgaaa gatgcagctc
     1081 atgctgataa tgttcgaact aaagatgaaa tcaatcgtca aaaacaagaa catgtcaaag
     1141 ataatgagga ggttaactct aatgttgctg tagcaaggtc tcagggacga tatacgacaa
     1201 atgatggtta tgtctttaat ccagctgata ttatcgaaga tacgggtaat gcttatatcg
     1261 ttcctcatgg aggtcactat cactacattc ccaaaagcga tttatctgct agtgaattag
     1321 cagcagctaa agcacatctg gctggaaaaa atatgcaaca gagtcagtta agctattctt
     1381 caacagctag tgacaataac acgcaatctg tagcaaaagg atcaactagc aagccagcaa
     1441 ataaatctga aaatctccag agtcttttga aggaactcta tgattcacct agcgcccaac
     1501 gttacagtga atcagatggc ctggtctttg accctgctaa gattatcagt cgtacaccaa
     1561 atggagttgc gattccgcat ggcgaccatt accactttat tccttacagc aagctttctg
     1621 ccttagaaga aaagattgcc agaatggtgc ctatcagtgg aactggttct acagtttcta
     1681 caaatgcaaa acctaatgaa gtagtgtcta gtctaggcag tctttcaagc aatccttctt
     1741 ctttaacgac aagtaaggag ctctcttcag catctgatgg ttatattttt aatccaaaag
     1801 atatcgttga agaaacggct acagcttata ttgtaagaca tggtgatcat ttccattaca
     1861 ttccaaaatc aaatcaaatt gggcaaccga ctcttccaaa caatagtcta gcaacacctt
     1921 ctccatctct tccaatcaat ccaggaactt cacatgagaa acatgaagaa gatggatacg
     1981 gatttgatgc taatcgtatt atcgctgaag atgaatcagg ttttgtcatg agtcacggag
     2041 accacaatca ttatttcttc aagaaggact tgacagaaga gcaaattaag gctgcgcaaa
     2101 aacatttaga ggaagttaaa actagtcata atggattaga ttctttgtca tctcatgaac
     2161 aggattatcc aagtaatgcc aaagaaatga aagatttaga taaaaaaatc gaagaaaaaa
     2221 ttgctggcat tatgaaacaa tatggtgtca aacgtgaaag tattgtcgtg aataaagaaa
     2281 aaaatgcgat tatttatccg catggagatc accatcatgc agatccgatt gatgaacata
     2341 aaccggttgg aattggtcat tctcacagta actatgaact gtttaaaccc gaagaaggag
     2401 ttgctaaaaa agaagggaat aaagtttata ctggagaaga attaacgaat gttgttaatt
     2461 tgttaaaaaa tagtacgttt aataatcaaa actttactct agccaatggt caaaaacgcg
     2521 tttcttttag ttttccgcct gaattggaga aaaaattagg tatcaatatg ctagtaaaat
     2581 taataacacc agatggaaaa gtattggaga aagtatctgg taaagtattt ggagaaggag
     2641 tagggaatat tgcaaacttt gaattagatc aaccttattt accaggacaa acatttaagt
     2701 atactatcgc ttcaaaagat tatccagaag taagttatga tggtacattt acagttccaa
     2761 cctctttagc ttacaaaatg gccagtcaaa cgattttcta tcctttccat gcaggggata
     2821 cttatttaag agtgaaccct caatttgcag tgcctaaagg aactgatgct ttagtcagag
     2881 tgtttgatga atttcatgga aatgcttatt tagaaaataa ctataaagtt ggtgaaatta
     2941 aattaccgat tccgaaatta aaccaaggaa caaccagaac ggccggaaat aaaattcctg
     3001 taaccttcat ggcaaatgct tatttggaca atcaatcgac ttatattgtg gaagtaccta
     3061 tcttggaaaa agaaaatcaa actgataaac caagtattct accacaattt aaaaggaata
     3121 aagcacaaga aaactcaaaa cttgatgaaa aggtagaaga accaaagact agtgagaagg
     3181 tagaaaaaga aaaactttct gaaactggga atagtactag taattcaacg ttagaagaag
     3241 ttcctacagt ggatcctgta caagaaaaag tagcaaaatt tgctgaaagt tatgggatga
     3301 agctagaaaa tgtcttgttt aatatggacg gaacaattga attatattta ccatcgggag
     3361 aagtcattaa aaagaatatg gcagatttta caggagaagc acctcaagga aatggtgaaa
     3421 ataaaccatc tgaaaatgga aaagtatcta ctggaacagt tgagaaccaa ccaacagaaa
     3481 ataaaccagc agattcttta ccagaggcac caaacgaaaa acctgtaaaa ccagaaaact
     3541 caacggataa tggaatgttg aatccagaag ggaatgtggg gagtgaccct atgttagatc
     3601 cagcattaga ggaagctcca gcagtagatc ctgtacaaga aaaattagaa aaatttacag
     3661 ctagttacgg attaggttta gatagtgtta tattcaatat ggatggaacg attgaattaa
     3721 gattgccaag tggagaagtg ataaaaaaga atttatctga tctcatagcg taaggaatag
     3781 cagtagaaaa agtctgaatc aaaaatgaag ttctctcaaa agttagaaat aaaactctga
     3841 cttt
//
LOCUS       DAVKAO010000103         3786 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKAO010000103 DAVKAO010000000
VERSION     DAVKAO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.103"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            68..295
                     /locus_tag="VWX16_001923"
     CDS             68..295
                     /locus_tag="VWX16_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159218.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            583..3645
                     /gene="nanA"
                     /locus_tag="VWX16_001924"
     CDS             583..3645
                     /gene="nanA"
                     /locus_tag="VWX16_001924"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9159219.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRIIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGVIG
                     LEFDSEVLVNKAPTLQLANGKTARFMTQYDTKTLLFTVDSEDMGQKVTGLAEGAIESM
                     HNLPVSVAGTKLSNGMNGSEAAVHEVPEYTGPLGTSGEEPAPTVEKPEYTGPLGTSGE
                     EPAPTVEKPEYTGPLGTAGEEAAPTVEKPEFTGGVNGTEPAVHEIAEYKGSDSLVTLT
                     TKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGMFTLGKKREQ"
BASE COUNT         1283 a          674 c          871 g          958 t
ORIGIN      
        1 aaatagtagt agctatgcga aataacagat agagagaagg gattgaagct tagaaaaggg
       61 gaataatatg atatttaagg cattcaagac aaaaaagcag agaaaaagac aagttgaact
      121 acttttgaca gtttttttcg acagttttct gattgattta tttcttcact tatttgggat
      181 tgtccccttt aagctggata agattctgat tgtgagcttg attatatttc ccattatttc
      241 tacaagtatt tatgcttatg aaaagctatt tgaaaaagtg ttcgataagg attgagcagg
      301 aagtatggtg taaatagcat aagctgatgt ccatcatttg cttataaaga gatattttag
      361 tttaattgca gcggtgtcct ggtagataaa ctagattggc aggagtctga ttggagaaag
      421 gagaggggaa atttggcacc aatttgagat agtttgttta gttcattttt gtcatttaaa
      481 tgaactgtag taaaagaaag ttaataaaag acaaactaag tgcattttct ggaataaatg
      541 tcttatttca gaaatcggga tatagatata gagaggaaca gtatgaatcg gagtgttcaa
      601 gaacgtaagt gtcgttatag cattaggaaa ctatcggtag gagcggtttc tatgattgta
      661 ggagcagtgg tatttggaac gtctcctgtt ttagctcaag aaggggcaag tgagcaacct
      721 ctggcaaatg aaactcaact ttcgggggag agctcaaccc taactgatac agaaaagagc
      781 cagccttctt cagagactga actttctggc aataagcaag aacaagaaag gaaagataag
      841 caagaagaaa aaattccaag agattactat gcacgagatt tggaaaatgt cgaaacagtg
      901 atagaaaaag aagatgttga aaccaatgct tcaaatggtc agagagttga tttatcaagt
      961 gaactagata aactaaagaa acttgaaaac gcaacagttc acatggagtt taagccagat
     1021 gccaaggccc cagcattcta taatctcttt tctgtgtcaa gtgctactaa aaaagatgag
     1081 tacttcacta tggcagttta caataatact gctactctag aggggcgtgg ttcggatggg
     1141 aaacagtttt acaataatta caacgatgca cccttaaaag ttaaaccagg tcagtggaat
     1201 tctgtgactt tcacagttga aaaaccgaca gcagaactac ctaaaggccg agtgcgcctc
     1261 tacgtaaacg gggtattatc tcgaacaagt ctgagatctg gcaatttcat taaagatatg
     1321 ccagatgtaa cgcatgtgca aatcggagca accaagcgtg ccaacaatac ggtttggggg
     1381 tcaaatctac agattcggaa tctcactgtg tataatcgtg ctttaacacc agaagaggta
     1441 caaaaacgta gtcaactttt taaacgctca gatttagaaa aaaaactacc tgaaggagcg
     1501 gctttaacag agaaaacgga catattcgaa agcgggcgta acggtaaacc aaataaagat
     1561 ggaatcaaga gttatcgtat tccagcactt ctcaagacag ataaaggaac tttgatcgca
     1621 ggtgcagatg aacgccgtct ccattcgagt gactggggtg atatcggtat ggtcatcaga
     1681 cgtagtgaag ataatggtaa aacttggggt gaccgagtaa ccattaccaa cttacgtgac
     1741 aatccaaaag cttctgaccc atcgatcggt tcaccagtga atatcgatat ggtgttggtt
     1801 caagatcctg aaaccaaacg aatcttttct atctatgaca tgttcccaga agggaaggga
     1861 atctttggaa tgtcttcaca aaaagaagaa gcctacaaaa aaatcgatgg aaaaacctat
     1921 caaatcctct atcgtgaagg agaaaaggga gcttatacca ttcgagaaaa tggtactgtc
     1981 tatacaccag atggtaaggc gacagactat cgcgttgttg tagatcctgt taaaccagcc
     2041 tatagcgaca agggggatct atacaagggt aaccaattac taggcaatat ctacttcaca
     2101 acaaacaaaa cttctccatt tagaattgcc aaggatagct atctatggat gtcctacagt
     2161 gatgacgacg ggaagacatg gtcagcgcct caagatatta ctccgatggt caaagccgat
     2221 tggatgaaat tcttgggtgt aggtcctgga acaggaattg tacttcggaa tgggcctcac
     2281 aagggacgga ttttgatacc ggtttatacg actaataatg tatctcactt aaatggctcg
     2341 caatcttctc gtatcatcta ttcagatgat catggaaaaa cttggcatgc tggagaagcg
     2401 gtcaacgata accgtcaggt agacggtcaa aagatccact cttctacgat gaacaataga
     2461 cgtgcgcaaa atacagaatc aacggtggta caactaaaca atggagatgt taaactcttt
     2521 atgcgtggtt tgactggaga tcttcaggtt gctacaagta aagacggagg agtgacttgg
     2581 gagaaggata tcaaacgtta tccacaggtt aaagatgtct atgttcaaat gtctgctatc
     2641 catacgatgc acgaaggaaa agaatacatc atcctcagta atgcaggtgg accgaaacgt
     2701 gaaaatggga tggtccactt ggcacgtgtc gaagaaaatg gtgagttgac ttggctcaaa
     2761 cacaatccaa ttcaaaaagg agagtttgcc tataattcgc tccaagaatt aggaaatggg
     2821 gagtatggca tcttgtatga acatactgaa aaaggacaaa atgcctatac cctatcattt
     2881 agaaaattta attgggactt tttgagcaaa gatctgattt ctcctaccga agcgaaagtg
     2941 aagcgaacta gagagatggg caaaggagtt attggcttgg agttcgactc agaagtattg
     3001 gtcaacaagg ctccaaccct tcaattggca aatggtaaaa cagcacgctt catgacccag
     3061 tatgatacaa aaaccctcct atttacagtg gattcagagg atatgggtca aaaagttaca
     3121 ggtttggcag aaggtgcaat tgaaagtatg cataatttac cagtctctgt ggcgggcact
     3181 aagctttcga atggaatgaa cggaagtgaa gctgctgttc atgaagtgcc agaatacaca
     3241 ggcccattag ggacatccgg cgaagagcca gctccaacag tcgagaagcc agaatacaca
     3301 ggcccactag ggacatccgg cgaagagcca gccccgacag tcgagaagcc agaatacaca
     3361 ggcccactag ggacagctgg tgaagaagca gctccaacag tcgagaagcc agaatttaca
     3421 gggggagtta atggtacaga gccagctgtt catgaaatcg cagagtataa gggatctgat
     3481 tcgcttgtaa ctcttactac aaaagaagat tatacttaca aagctcctct tgctcagcag
     3541 gcacttcctg aaacaggaaa caaggagagt gacctcctag cttcactagg actaacagct
     3601 ttcttccttg gtatgtttac gctagggaaa aagagagaac aataagagaa gaattctaaa
     3661 catttgattt tgtaaaaata gaaggagata gcaggttttc aagcctgcta tctttttttg
     3721 atgacattca ggctgatacg aaatcataag aggtctgaaa ctactttcag agtagtctgt
     3781 tctata
//
LOCUS       DAVKAO010000104         3775 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKAO010000104 DAVKAO010000000
VERSION     DAVKAO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.104"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(152..907)
                     /locus_tag="VWX16_001925"
     CDS             complement(152..907)
                     /locus_tag="VWX16_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEU9159220.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(977..3628)
                     /locus_tag="VWX16_001926"
     CDS             complement(977..3628)
                     /locus_tag="VWX16_001926"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9159221.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVVHDNVTKVATGEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(3640..>3775)
                     /locus_tag="VWX16_001927"
     CDS             complement(3640..>3775)
                     /locus_tag="VWX16_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEU9159222.1"
                     /translation="ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN
                     "
BASE COUNT         1043 a          844 c          766 g         1122 t
ORIGIN      
        1 ataaaagagt tataatcagc actactgata aattcaatac cgatttcgcc atcttaaaac
       61 ttagtcaaac gaataccgtt gatttccagt tcattaagtc gttgacctgt caagacttct
      121 cgttccttca actggaaaag ttaaggggga actaatacaa atcctctacg cttctatatt
      181 caatgatttc atttttgata atattctttt taaactcaaa atgttttaaa ggattatcga
      241 ttaacactaa ctttggaata tcatctagat ttgtgaccaa ggataaaata aaatccgatt
      301 gaaactcctt gactttttca aattcttttg cagttaaacg cacacgcccc ttagattttc
      361 ggataccttt tacttcggca aggtaagaat actcttggac atctacctga aagtcatatc
      421 catcaccata aagacgggca tcggttaact gtccaccttg gaatttttct tctttatcaa
      481 aatgaaggat aaaataattc tcagcctcca ttcccgtttc ctgtagacgt ttgaatttag
      541 ttcgaactat tggtttttca acgatggtta tgcctgtttt cttcccttca ctggcaagta
      601 acattttaac tatttcagca taactatgaa catcttcatt tccaaacatc atatcaatta
      661 aatctttacg aaaacgataa acttcagctt tctgccacca accttttcga ttattgtcaa
      721 aataaggatc aaataaatcc attctatttt ttacaactcc agttgtttca gcaattccca
      781 gagaaaccac atagcgataa aactccgact tgctagagca ttgaaattct tttataaaaa
      841 gcttgtcaaa cttggcaaga ccgtagccaa ttaaatttaa caattcataa tgagggtgtt
      901 tggacatcac tttctccatc aatagatatt gatttcatta taccaaaaaa cacggctcat
      961 caccgtgttt ctgtgtttat ttcaccaact tcttcatctc atcaatacgt gctacggtcg
     1021 catcatactt ggcttggtag tcggcttgtt tgtctttttc tttttggaca acttctggtt
     1081 tagcgttggc gacgaagcgt tcgttgctga gttttttgcc aaccatgtcg agttctttct
     1141 gccatttggc aagttctttt tccagacgtg ccaattcttc ttcgacattc aagaggtcta
     1201 ccagtggcaa gaagatttcg gctcctgtaa taacgcttga catagccagt tcaggtgcag
     1261 ggatggttga tgcgatttcc aagtgttctg gatttgtaaa gcgtttgata tagttgacat
     1321 tgctgttaaa gaaggcttcc aagtcgctat cgcttgtctt aacaaggatg gtgataggtt
     1381 tgcttggtgc tacatttact tccgcacgcg cattacgaac ggcacggatc aagtctttga
     1441 gactttccac accagtgtgg gcagcaaggt cctcaaaggc tgggttgaca gttgggtatg
     1501 ctgctgtcac gatagagcct tcagcgtact gaccaaagat ttcttctgtc acgaatggca
     1561 tgattgggtg aagaagacgg aggattttat ccagcgtata gaggagaaca gatcgagtaa
     1621 tgaccttatc gtcttcattg tcgctgtaaa gaacttcctt ggtcaactca acataccagt
     1681 tggcaaattc ttcccaaatg aagttgtaga ggatgtggcc agcaacacca aactcaaact
     1741 tatcaaagtt agcagttgct tttccaatcg tttcattgag gttgtggaga atccagcggt
     1801 ccgtcacatt accagcctca cctgttgcaa cttttgtgac attgtcatgc accacatcca
     1861 gcgtcaaacc ttcattgttc ataaggatgt agcgagagat gttccagatc ttgttaatga
     1921 agttccatga agcatccatt ttctcataag agaaacgcac gtcttgtcct ggcgcagaac
     1981 cgtttgaaag gaaccatcga agggcatcgg caccgtattt ctcgataaca tccattgggt
     2041 caatcccgtt accgagagac ttagacatct tgcgtccttg ctcgtcacga ataagaccgt
     2101 ggataaggac gttttggaat ggttgacggc ctgtgaattc caatgactgg aagatcatac
     2161 gagacaccca gaagaagatg atgtcgtaac ctgttaccaa ggttgaagtt gggaaataac
     2221 gtttaaagtc ttctgagtcg acttcaggcc agcccatggt tgaaaatggc cagagggcag
     2281 aactgaacca agtatccaag acgtcttcgt cctgagtcca tccgtcacct tctggagctt
     2341 cttcgccgac atacatttca ccatcagcat tgtaccaggc agggatttgg tgaccccacc
     2401 aaagctgacg agagataacc cagtcgtgga cattttccat ccattgaagg aaggtatcgt
     2461 tgaaacgagg tgggtagaat tcgaccttgt cctctgtgtc ttggttggca atggcgttct
     2521 tagccaattg gtccatcttg acgaaccatt gagtagacaa gcgtggctca actacgacac
     2581 ctgtccgttc tgagtgaccg actgaatgga cacgtttttc gattttgaca agggcaccga
     2641 tttcttccaa cttagcaacg actgccttac gagcttcaaa acgatccatg cctgaaaatt
     2701 caaaggcaag ctcattcata gttccgtcgt cgttcatgac gttgacttgt ggcaagttat
     2761 gacgttggcc aaccaagaag tcatttggat cgtgggcagg tgtgattttc acgacaccag
     2821 taccaaactc aggatctgcg tgctcatctc caacgattgg gatgagttta ttagcgattg
     2881 gaaggatgac gtttttacca atcaagtcct tgtagcgcgg gtcttctgga ttaaccgcaa
     2941 ccgcaacgtc cccaaacata gtctcaggac gagttgtagc aacttcaagg gcgcgtgaac
     3001 catcttccag catgtaattc atgtggtaga aggcaccttc cacatccttg tgaatcacct
     3061 caatatcaga aagggctgtg cgagctgctg ggtcccagtt gatgataaac tcaccacggt
     3121 agatccagcc tttcttgtaa aggtccacaa agaccttacg aacagctttt gacaaacctt
     3181 cgtcaagagt gaaacgctca cgagaatagt ctacagagag ccccatcttg ccccattgtt
     3241 ccttgatggt agtggcatat tcgtctttcc attcccagac tttcgtcaag aaagactcac
     3301 gaccaaggtc atagcgggaa atgccctcac cacgcaagcg ctcctcaacc ttagcctgag
     3361 tcgcaatacc agcgtggtcc attcctggaa gccaaagggt atcaaaacct tgcatgcgtt
     3421 tttgacggat gataatatct tgcaaagttg tatcccaagc gtgaccaagg tgaagtttac
     3481 ctgtaacgtt tggtggtgga ataacgattg aataaggctt agccttttga tcgcctgaag
     3541 gcttgaaaac atcagcatca agccattttt ggtaacgacc agcctcaacc tcggctggat
     3601 tgtatttaga tgaaagttct ttagacatgt gtgttctcct taattacttt tattgacaat
     3661 tttaaccaaa ccaatttttg aaagttcaat ataaatctct tcaatttcag caaatgctgc
     3721 aagtggtgac atagtataat ttccaggatt atatttaaga tcatacttgc ttatc
//
LOCUS       DAVKAO010000105         3469 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKAO010000105 DAVKAO010000000
VERSION     DAVKAO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.105"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            165..2798
                     /gene="polA"
                     /locus_tag="VWX16_001928"
     CDS             165..2798
                     /gene="polA"
                     /locus_tag="VWX16_001928"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9159223.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGVKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHIELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     VVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2883..3320
                     /locus_tag="VWX16_001929"
     CDS             2883..3320
                     /locus_tag="VWX16_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9159224.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          943 a          608 c          886 g         1032 t
ORIGIN      
        1 caaggtgtac tattatagat tcaatatatt atgttatagc acatttttaa atctaggttt
       61 actaaattct ctggctctat ctatttattc ggaaaaagtt tgaaaaatac ttgctctagc
      121 tcttcccaat ggtatttttt gattctttcc tttataatgg gtgtatggat aagaaaaaat
      181 tattattgat tgatgggtct tctgtagctt ttcgggcgtt ttttgcgctg tatcagcagt
      241 tggaccgttt taagaatgcg gctggtttgc ataccaatgc gatttatggt ttccagttga
      301 tgttgagtca tttgttggag cgggttgagc cgagtcatat tttggtggct tttgatgcgg
      361 gaaagacgac cttccggaca gagatgtatg cggactataa gggtggtcgg gccaagactc
      421 ctgatgagtt tcgtgagcaa tttcctttca ttcgtgagtt gctggatcat atggggattc
      481 gtcactatga gctggctcag tatgaggcgg atgacatcat tgggacgctg gataagctag
      541 cagagcagga tggttttgat attaccattg tcagtgggga caaggatttg attcagctga
      601 cggatgagca tacggtggtt gaaatttcca agaaaggtgt ggctgagttt gaggccttta
      661 cgccagatta cctcatggaa gaaatgggcc tcacaccagc tcagtttatc gatctcaagg
      721 cgctcatggg tgataagtcg gataatatcc ctggggtgac caaagtcggt gaaaagacgg
      781 gtgttaagct cttgctggag catggttcgc ttgaggggat ttatgaaaat attgatggaa
      841 tgaagacttc taagatgaag gaaaatctca tcaatgacaa ggaacaggcc tttttgtcta
      901 aaacactagc gaccattgat accaaggcac cgattgcgat tggtttagag gacttggtct
      961 atagtggtcc agatgttgaa aatcttggga aattttacga tgagatgggc ttcaaacagc
     1021 tcaagcaggc tttaaatgtg tcgtcagctg atgtggctga gggtttggat tttactattg
     1081 ttgaccaaat cagtcaagat atgctgagtg aagagtctat cttccacatt gagctttttg
     1141 gtgagaatta ccatacggat aatttggttg gatttgcctg gtcttgtggg gataagctct
     1201 atgccacaga caagcttgag ctgttgcaag acccgatttt caaggatttc ttagaaaaaa
     1261 catctctgag agtttatgac tttaagaagg ttaaagttct tttgcaacgt tttggtgtgg
     1321 atttgcaggc gcctgctttt gacatccgtt tggctaaata cctcctttcg actgtggagg
     1381 acaatgaaat tgcgaccatc gctagtcttt atggtcagac ttacttggtt gatgatgaaa
     1441 ctttctacgg taagggtgtt aaaaaggcca ttcctgaacg tgagaaattc ttggaacact
     1501 tagcttgtaa acttgttgtt ttggtagaaa cagagcctat tttacttgaa aaactcagcg
     1561 aaaatgggca attagagctt ctttatgata tggagcaacc tctggctttt gtccttgcca
     1621 agatggaaat tgctgggatt atggtcaaga aagagacctt gcttgagatg caggctgaaa
     1681 atgagcttgt cattgaaaaa ctgactcaag agatttacga gctggctggt gaggagttta
     1741 atgtcaactc gcctaagcag ttgggcgtgc ttctctttga gaaattggga cttcctctag
     1801 aatacactaa gaaaaccaag acaggttatt cgacagcagt ggatgttttg gagcgtctcg
     1861 ctcctattgc tccgattgtt aagaaaatcc tggattaccg tcaaattgct aagattcaat
     1921 ctacttatgt aattggcttg caggactgga ttttggctga tggaaagatt catactcgct
     1981 atgtgcagga tttgacccaa accgggcgtt tgtctagtgt ggatccaaac ttgcaaaata
     2041 ttcctgcccg attggaacag gggcgcttga ttcggaaggc ttttgtgcca gagtgggagg
     2101 atagtgtgct actcagctct gactattcac agattgaatt gcgcgttttg gcgcatattt
     2161 ctaaagatga gcacttgatt aaggccttcc aagagggggc agatatccat acttcgacag
     2221 ccatgcgggt ctttggcatt gagcgtcctg atgatgtgac tgcaaacgac cgtcgcaatg
     2281 ccaaggcagt taactttgga gtggtttatg ggatttcaga ctttggcttg tctaataatt
     2341 tgggaattag tcgtaaggaa gccaaagcct acattgatac ctactttgaa cgttttccag
     2401 gtattaaaaa ctacatggat gaagtggtgc gggaggcgcg tgataagggc tatgtagaga
     2461 ccctctttaa gcgtcgccgt gagttgccag atatcaattc gcgcaacttc aatattcgtg
     2521 gttttgcgga gcgaactgct atcaactcac ctatccaggg ttcggcagca gatattctca
     2581 agattgccat gattcagctg gataaagcct tggttgcagg tggttatcag actaagatgc
     2641 tgttacaagt gcacgatgaa atcgtccttg aagtgcctaa atctgaattg gtagagatga
     2701 aaaaattggt gaaacaaacc atggaagaag ccattcaact cagtgttcct cttatcgcag
     2761 atgagaatga aggggcaacc tggtacgagg ctaaataaaa agggggctag tcctcctttt
     2821 ttgtagtaga attctgcaaa ccttttcaaa atgtgctata ctgatgaaaa aggaggattt
     2881 ctatgagtca agaatttatc aatccaagtg atggcgtgat tcgtcagtat ctcgcaacga
     2941 gtaaaaccct tgctgtggtg ggtttgtctg accgtgaaga aacaaccagc aatcgagtaa
     3001 caaaggaaat gcaggctcgg ggctataaaa tcatcccagt caatcccaag gcggcaggtg
     3061 gcgaaatctt gggtgaaaag gcttatgcta gcctcgctga aattcctttt cctgtagata
     3121 ttgtcaatgt ttatcgtcgc agtgaatttc tgcccgatgt ggcgcgtgat tttctcaaag
     3181 ctgatgccaa gattttttgg gcacagctag gacttgaaag tttagaagcg aaagagattt
     3241 tgcgtgatgg tggatgtgat gatatcgtga tgaatcgctg tatcaagaga gaacatactc
     3301 gcttgattga ggaagcataa gaaaaaggta gctgatgggc taccttttgt gttatactca
     3361 atgaaaatca aagagcaaac taggaaacta gctgcgggtt gctcaaaaca ctgttttgag
     3421 gttgtagata gaactgacga agtcagctca aaacaccgtt ttgaggttg
//
LOCUS       DAVKAO010000106         3390 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKAO010000106 DAVKAO010000000
VERSION     DAVKAO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.106"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            217..480
                     /locus_tag="VWX16_001930"
     CDS             217..480
                     /locus_tag="VWX16_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9159225.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            486..2219
                     /gene="ptsP"
                     /locus_tag="VWX16_001931"
     CDS             486..2219
                     /gene="ptsP"
                     /locus_tag="VWX16_001931"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9159226.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2474..>3390
                     /gene="phtA"
                     /locus_tag="VWX16_001932"
     CDS             2474..>3390
                     /gene="phtA"
                     /locus_tag="VWX16_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9159227.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRENLSNLRTYRRQNSDNTPRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVES"
BASE COUNT         1129 a          642 c          732 g          887 t
ORIGIN      
        1 tgaaatagca atgattatcc tataaattgt ttttgaagaa actatatcct gtgttttgtt
       61 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
      121 atgcttgaaa aaaatcctag aagatggtac aatactaaat tgtaagggtt atcacatata
      181 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
      241 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
      301 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
      361 ggtgttatga gtcttggtgt tggtcaaggt gctgacgtaa ctatctcagc tgaaggtgca
      421 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
      481 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
      541 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
      601 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
      661 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
      721 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
      781 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
      841 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
      901 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctatcaat gaagaagtga
      961 ttgtgattgc gcatgacttg actccttcag atacagctca attggacaaa aactttgtaa
     1021 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
     1081 ttgaaattgc tgctgtatta ggtacaaaca acatcactga aatcgttaaa gacggtgaca
     1141 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
     1201 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
     1261 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
     1321 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
     1381 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
     1441 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
     1501 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
     1561 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
     1621 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
     1681 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
     1741 ctgaaggtgt tgcagttgcg gataacattc aagttggtat catgatcgag attcctgcag
     1801 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaatg
     1861 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
     1921 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
     1981 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
     2041 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
     2101 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
     2161 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
     2221 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
     2281 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
     2341 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
     2401 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
     2461 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
     2521 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
     2581 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
     2641 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
     2701 aggctatgtc acttcacatg gcgaccacta tcattattac aatggtaagg ttccttatga
     2761 tgcgattttc agtgaagaat tactcatgaa agatccaaac tataagctaa aagatgagga
     2821 tattgttaat gaggtcaagg gtggatatgt tatcaaggta gatggaaaat actatgttta
     2881 ccttaaggat gctgcccacg cggataacgt ccgtacaaaa gaggaaatca atcgacaaaa
     2941 acaagaacat agtcagcatc gtgaaggagg gacttcaaca aacgatggtg cggtagcctt
     3001 tgcacgttca cagggacgct acaccacaga tgatgggtat atcttcaatg catctgatat
     3061 cattgaggac actggtgatg cttatatcgt tcctcatgga gatcattacc attacattcc
     3121 taagaatgag ttatcagcta gcgagttggc tgctgcagaa gccttcctat ctggtcggga
     3181 aaatctgtca aatttaagaa cctatcgccg acaaaatagc gataacactc caagaacaaa
     3241 ctgggtacct tctgtaagca atccaggaac tacaaatact aacacaagca acaacagcaa
     3301 cactaacagt caagcaagtc aaagtaatga cattgatagt ctcttgaaac agctctacaa
     3361 actgcctttg agtcaacgac atgtggaatc
//
LOCUS       DAVKAO010000107         3340 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKAO010000107 DAVKAO010000000
VERSION     DAVKAO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.107"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(189..800)
                     /gene="rpsD"
                     /locus_tag="VWX16_001933"
     CDS             complement(189..800)
                     /gene="rpsD"
                     /locus_tag="VWX16_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9159228.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(995..2047)
                     /locus_tag="VWX16_001934"
     CDS             complement(995..2047)
                     /locus_tag="VWX16_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9159229.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2044..2742)
                     /locus_tag="VWX16_001935"
     CDS             complement(2044..2742)
                     /locus_tag="VWX16_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9159230.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2909..>3340)
                     /locus_tag="VWX16_001936"
     CDS             complement(2909..>3340)
                     /locus_tag="VWX16_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU9159231.1"
                     /translation="GTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTAN
                     GSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMISE
                     DKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          923 a          748 c          643 g         1026 t
ORIGIN      
        1 ccataaaaga ttataccaca ttgtgtacta tttttggttc attttactat atcactttgg
       61 actatatcgg tctagttatg tctcaacgat tatagctgga tactaagaga aatcaaaaaa
      121 gcactctagg atagaggcct aaagtgctta gtttcaaggc tttacagcct atcatattta
      181 ataaaatatt acaacatctt gttgtagaat tcaacgacaa gtgcttcgtt gatttctggg
      241 ttgatttcgt cgcgttctgg caagcgagtc aatgaacctt ccaatttttc agcgtcgaat
      301 gatacgaatg ctggacgtcc aagagtagct tctactgctt caaggattgc tggaactttc
      361 aatgattttt cacgaactga gatcacttga cctggagtta cgcggtatga tgggatatca
      421 acgcgtttcc cgtcaacaag gatgtgaccg tggtttacga attgacgagc ttgacgacga
      481 gtagtcgcga gaccaagacg gtaaacaacg ttatccaaac gacgttccaa aagaagcata
      541 aagttgaaac ctaggattcc gcctttgatt tttgtagctt gtacgaacaa gttacggaat
      601 tgtttttcac ctacaccgta agtgaaacga agtttttgtt tttcagccaa ttgcaaaccg
      661 tattctgaca atttagaacg gttgtttggt ccgtgttgtc ctggtacgta gttacgacgt
      721 gccaattctt tacctgtacc tgtaagtgaa aggccaaggc gacgagcttg tttccaagat
      781 ggtcctgtat aacgtgacat atgaatgtcc tcctgatata aataatattt cggtggaaat
      841 agtcacttag aaagccctga ttcgtgcaga tgcccttcgc ctaaacagcc aaggttactt
      901 gtcataagac acctgttgac gagcttcatg ctttcctgct gatatttcac acaaaattca
      961 ttctaccatg aatagctgga tttgtaaagg ggttttaggc tttattttca ctaccagaga
     1021 gattgagaac gagggtaaag gtacttccta gaccgtactg gctgctgact gtgatttccc
     1081 cacccaattg atgggccaat tcacgcgcaa tcgcaagtcc taatccatga ccacctgtct
     1141 tcatgttacg cgaagtttcg acacgataaa ggcgtttgaa aatattttcc aaatcctctg
     1201 gggcaatgcc ctgcccttca tcggtcacac tgattgaaag ctggcccttc tccagcttag
     1261 tcaccacttc cagcttggtt cctggagcag aatatttaaa agcgttatcg accagattca
     1321 ccaagatacg agaaagctta gcataatctc cctcaatccg ggcagactct gggattacct
     1381 gcaagtggac atctcttctc tcctgctcaa tcaaaaactg aaattcactc atgcactcaa
     1441 ttaagagctt gtccagaaaa atactgtctt tactggtagt ttccacctga tttctagctg
     1501 tgtttagggt caaaaaattc aactcctcaa ccagtttatt gagcctctcc gtctggcgtc
     1561 caatggttgc tagataatga gcttgctccg actccttgat aatcccatcc aaaatccctt
     1621 ctaccgtcgc ttggatcgaa gtgatagggg tcttaatatc atgcgacaac tgggcaatca
     1681 tcaagccctt ttctcgttcg ctttcttcca aggaatcaaa gcttacctgc aaatcatggg
     1741 acatctcatt aaaagtttgc cctaattgct gaaattctac aggaccttga acctccaaat
     1801 ttgaaggaaa atccttggcc gctacccgct tggcatgctc cttgagtttg cccaacgacg
     1861 taaagactgg caataggaga aagagactaa tcccagcacc gacaaggctg gcaacgatgg
     1921 tcatcccaag caaaaagtaa atctcgcctt tcgcaatcag cattttttga acagcccaaa
     1981 aaacaaccaa aatggttaag agggttgaaa taatatatcc aaccaaaata taacttttta
     2041 gtttcatgtt tgtcctctcg gtttctctat cttatatccc aacccccaaa ctgtcttaat
     2101 agtgggcgtt tggtcactac tatattttgc cagctcctgt cgaagagcat ggatatgcac
     2161 attcaaggta ttggtgtcat ccacgtagtc ttctttccag atcttttcat agaggtctgt
     2221 cttggagaaa actcgctctg gattgctagc taaaatccac agcaattcaa atgatttaac
     2281 agttaaatcc agcatttctt ctcctatttg aacttcatga ctactatgat tcatttttag
     2341 attgccaagg gaaatcagct ctgtttcgcc cccacgatga aggcggcgca aaatattgtg
     2401 gacacgcaaa accagctcac gtgggctaaa aggcttagca ataaaatcat ctgctcccaa
     2461 gctcaggccg taaatcttgt cctgttcact ggtcttagca gtaataaata ggaaaggctg
     2521 ctctggtgat aagtattgaa cctcactgat taaatcataa ccatccatcc gaggcatcat
     2581 gacatctgtg ataatcaaat caatcggttt tttcttgaac agctctagcg cttccagtcc
     2641 atcatgggct accaagacct gataacctgc ctgaattaag tatctctgat gaatatctgt
     2701 gatttctacc tcgtcgtcaa cgagtaaaat tgtctttccc atctgtctct cctttgataa
     2761 aaacagtgct atactgcttt aagtataaca ctatttttat tgttttacga tgattttaat
     2821 ctgctattga aagaaagtcc tgattgctga ttgagttgtt aaactaatct tagtcagccc
     2881 actatccatc atttatttcc ttagctgatt agttttcttc ttttttgttt ttcaaaccta
     2941 gaccaccgag taaacctgca agcgcaagcc caaggaaacc aatacttgcc attgatgttt
     3001 gagtctcacc agtatttggt agcatagctt tatcctctga tatcatcgta tcagacatct
     3061 tgttagcaga agcagccatg ttttcacctg ccatcgtgtt ggtagaactt gtcatggtgt
     3121 cagcaggcat gctatctgta gtacctgtag catgattgtg attcatcgga gtcacgccag
     3181 aaccagagtt agaaggtgat aatgaaccat ttgctgtgtc tgatgtttct ttaacattta
     3241 tcttaatagt gactttttta gttgctacga tgttgtccaa gtctggttta ccatctttgt
     3301 taccatagac attgactgta gcgctgtaag tttgagtacc
//
LOCUS       DAVKAO010000108         3309 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKAO010000108 DAVKAO010000000
VERSION     DAVKAO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.108"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            177..1383
                     /locus_tag="VWX16_001937"
                     /pseudo
     CDS             177..1383
                     /locus_tag="VWX16_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1385..2115
                     /locus_tag="VWX16_001938"
                     /pseudo
     CDS             1385..2115
                     /locus_tag="VWX16_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
     gene            2124..2816
                     /gene="cpsC"
                     /locus_tag="VWX16_001939"
     CDS             2124..2816
                     /gene="cpsC"
                     /locus_tag="VWX16_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU9159232.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVA
                     TLEEARPAISPSSPNIKRNTLIGFLAGGIGTSVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLGKLK"
     gene            2826..3243
                     /locus_tag="VWX16_001940"
                     /pseudo
     CDS             2826..3243
                     /locus_tag="VWX16_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
BASE COUNT         1079 a          526 c          693 g         1011 t
ORIGIN      
        1 ttagattgaa actagaatag tacacctctg cttctaaaac attgttagaa atcgatttga
       61 ctgtcctgat cgatttgtcc tgttcttatt tcattttact atatttttgg ttcgcgggaa
      121 gtctactaag atacttaaag atacagatag tgaaaataaa ggtgtagaca ttaccgttgg
      181 tgttctctat ccttgtcagc tctgtgtcgc tctttgcagt acagcagttt gttggactga
      241 ccaatcgttt aaatgcgact tctaattact cagaatattc gatcagtgtc gctgttttag
      301 cagatagtga gatcgaaaat gttatgcaac tgacgagtgt gacagcaccg actgggactg
      361 ataatgaaaa tattcaaaaa ttactagctg atatcaagtc agaataccga tttgacggtc
      421 gaccagagtt cgtcttactt ggctgcttac aagagtctga ttgcaggtga caccaagacc
      481 attgtcctaa atagtgtctt tgaaaatatc atcgagtcag agtatccaga ctacgcatcg
      541 aagataaaaa atatttatac caagggattt actaaaaaag tagaagctcc taagacgtct
      601 aagaatcagt ctttcaatat ctaagttagt ggaattgaca cctatggtcc tattagttcg
      661 gtgtcgcgat cagatgtcaa tatcctgatg actgtcaatc gagataccaa gaaaatcctc
      721 ttgaccacaa cgccgcgtga tgcctatgta ccaattgcag atggtggaaa taatcaaaaa
      781 gataaattga ctcatgcggg catttatgga gttgattcgt ccattcacac cttagaaaat
      841 ctctatggag tggatatcaa ttactatgta cgattgaact tcacttcgtt tttgaaattg
      901 attgatttgt tggatggaat tgatgtttat aatgatcaag aatttactgc ccatacgaat
      961 ggaaagtatt accctgaagg caatgttcat cttgattcag aacaggctct cggttttgtt
     1021 cgtgagcgct actccctagc agatggcgat cgtgaccgcg ggcgcaatca acaaaaggtg
     1081 attgtggcta tccttcaaaa attaacgtca accgaagaac tgaaaaatta tagtacgatc
     1141 attgatagct tgcaagattc tatccaaaca aatatgccga ttgagactat gatagattta
     1201 gtgaatactc agttggaaag tggagggaat tataaagtaa attctcaaga tttaaaaggg
     1261 acaggtcgga tggatcttcc ttcttatgca atgccagaca gtaacctcta tgtgatggaa
     1321 atagatgata gtagtttagc tgtagttaaa gcagctatac aggatgtgat gaaaggtaga
     1381 tgaaatgata gacatccatt cgcatatcgt ttttgatgta gatgacggtc ccaagtcaat
     1441 agaggatagc aaggcacttt taagagaagc ttataatcaa ggagttcgaa tgattgtgtc
     1501 tacttcgcat cgtcgaaaag ggatgtttga aactccagaa gaaaaaattg taacaaattt
     1561 tattaaggtt cgtgaaattg caaaagaagt agcagatgat ttagtcattg cttatggcgc
     1621 agagatatac tatactctgg atgctctaga aaagctagaa aaaaagaaat tcctaccctt
     1681 aatgatagtc gttatgcttt gattgagttt agcatgcata cttcctatcg tgagattcat
     1741 acgagattga gcaatatttt gatgttggga atcacgccag taattgctca tattgaacgt
     1801 tatgatgctt tagagaataa cgaaaaacgt gttcgtgaac tgattgatat ggggtgctat
     1861 actcagatag atagttatca tgtttcaaaa cctaagttct ttggtgaaaa atataaattc
     1921 atgaaaaaga gagctcggta ttttttggaa cgtgatttag ttcatgtagt tgcaagtgac
     1981 atgcacaatt tagacagtag acctccatat atgcaacagg catatgatat cattgctaag
     2041 aaatatagag cgaaaaaagc gaaagaactt tttgtagata atcccagaaa aattataatg
     2101 gatcaattaa tttaggagaa aatatgaagg aacaaaacac tttggaaatc gatgtattgc
     2161 aactattcag agctttatgg aaaagaaagt tggtcatttt attagtggca attataactt
     2221 cttcagttgc ttttgcctac agtacttttg ttatcaaacc tgagtttact agtacgactc
     2281 ggatttatgt agttaaccgt aatcagggag agaagtctgg tttaaccaat caagacttgc
     2341 aggcaggatc atacttggtt aaagactatc gtgaaattat cctatcgcag gatgttttgg
     2401 aggaagttat ttctgatttg aaactagatt tgacgccaaa aggtttggct aataaaatta
     2461 aagtaacagt accagttgat acccgtattg tctctgtttc agttaatgat cgagttcctg
     2521 aagaggcaag ccgtatcgct aactctttga gagaagtagc tgctcaaaaa attatcagta
     2581 ttactcgtgt ttctgatgtg gcaacactgg aggaggcaag accggcgata tcaccgtctt
     2641 cgccaaatat taaacgcaat acactaattg gttttttggc aggggggatt ggaactagtg
     2701 ttatagttct tcttcttgaa cttttggaca ctcatgtgaa acgtccggaa gatatcgaag
     2761 atacactgca gatgacactt ttgggagttg taccaaactt gggtaagttg aaataggaga
     2821 gaggaatgcc gacattagaa atagcacaaa aaactggagt tcattaagaa ggcagaagaa
     2881 tattacaatg ccttgtgtac aaatatacag ttgagcggag ataaactaaa agtaatttcc
     2941 gttacttctg ttaaccctgg ggaaggaaaa acaactactt ccgtaaatat agcaaggtcg
     3001 tttgcgcgtg caggctataa aactcttttg atcgatggtg atactcgaaa ttcagttatg
     3061 tcaggatttt ttaaatctcg tgaaaaaatt acagagctaa cagaattttt atctgggata
     3121 gctgatttat ctcacggttt atgtgataca aatattgaaa atttatttgt agttcaatcg
     3181 ggatctgtat caccaaaccc tacagccttg ttacaaagta aaaattttgt ggttatggta
     3241 aagatttttt caaaagaggt cagtatattg agttggtgga aacgataaaa tacataatat
     3301 tttattcct
//
LOCUS       DAVKAO010000109         3281 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKAO010000109 DAVKAO010000000
VERSION     DAVKAO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.109"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..175
                     /locus_tag="VWX16_001941"
     CDS             <1..175
                     /locus_tag="VWX16_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9159233.1"
                     /translation="NRTISFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVTL
                     ITGKNGTGKSTLL"
     gene            complement(541..1851)
                     /locus_tag="VWX16_001942"
     CDS             complement(541..1851)
                     /locus_tag="VWX16_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9159234.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2088..2960
                     /locus_tag="VWX16_001943"
     CDS             2088..2960
                     /locus_tag="VWX16_001943"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9159235.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     LLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3091..>3281
                     /locus_tag="VWX16_001944"
     CDS             3091..>3281
                     /locus_tag="VWX16_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9159236.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVKGTKPRKVDRD"
BASE COUNT         1025 a          550 c          741 g          965 t
ORIGIN      
        1 aaacagaact atttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat
       61 ttcgtatagg gtggatcatc gtatattgtt tgataatatt tcttttgata cttcgagttc
      121 aggcgtgaca ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtagat
      181 tgaaactaga atagtacaca tctacttcta aaatattgtt agaaatcgat ttgactatcc
      241 tgatctattt gtcctgttct tatttcattt cactacatct caaattgagt atgacgaagt
      301 gcgctcccat gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga
      361 taaagactat tgggatgaat ttttaaagtt gaactaatta tttttacagg atgaggttta
      421 cagcagagag tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt
      481 tccacttaat tttgtgtcta ctttattata ccaagtccac ccttaagtta gataatgaat
      541 ctaacttaag gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct
      601 gatagggaca cccagaataa tttgcttttt gaaggcaaga ccgcgttcct ctatattggg
      661 gagtgagagt tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc
      721 gccaataacg gtggctgttg tgagtaagtg aatatcaatc tgaggatttt gagcactgat
      781 gatagcaatg atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga
      841 catcactccg gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggttgttgt
      901 catgaggtgc cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct
      961 catgccgcag agcatgataa ttgtagccca gggaacctta gctaaaatgg cttcttgctt
     1021 ccctaatttg agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa
     1081 tgttttttga taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa
     1141 aagccaaacc aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct
     1201 gaggagtggt ggttggtcaa tagtcaagga tgagtttgtt cttcccttac tatagtggct
     1261 gtaacaggat aataaaagca agacgatgag tgggtagata atgctgacga taaagatatg
     1321 attgccaagt gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac
     1381 aatgcctgag ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacggcttg
     1441 agctccaatc aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta gaggacagca
     1501 aacggccata gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta
     1561 taaaatcatg tagagggcgt taggatgggt gcgtgtgcgg tagagaatgt gttgagccaa
     1621 gacatcaaga gtaccgttag ttgttgcaac gttataaaag agagagacgc taaaaatggt
     1681 aaaaaagagt gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt
     1741 ggttgcgatg aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta
     1801 accaattcca atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc
     1861 ctttcagtta gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc
     1921 ctattggtac ttgactatta tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac
     1981 acttgttaga attaaaaatg aggtattcag gcttgtgatt tcctatggaa gttcatagtg
     2041 attgcctcta atgcttacaa gtgatattaa aaatagagga tctagtgatg tcaatcattt
     2101 caactgattt aacccctttt caaatagatg atacattgaa agcagccttg cgagaagatg
     2161 ttcattccga agattacagt accaatgcca tttttgatca tcatggccaa gccaaggtgt
     2221 tgctttttgc caaggaagct ggtgttttag cggggctaac cgtttttcaa agggttttta
     2281 ccctatttga tgccgaggtg accttccaga atcctcatca atttaaggat ggggatcgtt
     2341 tgactagtgg cgatttggtt ttagaaatca taggctcggt gagaagtctc ttaacatgtg
     2401 aacgcgttgc cttgaatttt ttacaacatt tatcagggat tgcttcgatg acagctgctt
     2461 atgtagaagc cttaggcgat gattgcatta aggtatttga tactcgaaaa actactccta
     2521 atttacgtct ttttgagaaa tatgccgtga gagttggcgg tggctataat catcgcttta
     2581 atttatcaga tgctatcatg ctaaaagaca atcacattgc ggcagtaggt agtgttcaaa
     2641 aggcaattgc tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg
     2701 aaagccttgc tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc atgttggata
     2761 atatgtcatt ggaacagatt gaacaggcca ttaccctaat tgcaggacgt tctcggattg
     2821 aatgttctgg aaatattgat atgaccacta ttagccgttt tcgtggttta gcgattgatt
     2881 acgtctccag tggtagttta acccatagtg ctaagagtct tgatttttcc atgaaggggt
     2941 taacctacct tgatgtctaa gttgtaaaat aaactaactt tttaaaggat gtctttcctc
     3001 tagaacgagt tttatgtcag atagtttaaa cgcctcttca aatatagtaa aatgaaccaa
     3061 aaatagtaca caatgaggta taatcttctt atggcatatt caatagattt tcgtaaaaaa
     3121 gttctctctt attgtgagcg aacaggtagt ataacagaag catcacacgt tttccaaatc
     3181 tcacgtaata ccatttatgg ctggttaaag ctaaaagaga aaacaggaga gctaaaccac
     3241 caagtaaaag gaacaaaacc aagaaaagtt gatagagata g
//
LOCUS       DAVKAO010000110         3137 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKAO010000110 DAVKAO010000000
VERSION     DAVKAO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.110"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..242
                     /locus_tag="VWX16_001945"
     CDS             <1..242
                     /locus_tag="VWX16_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159237.1"
                     /translation="KICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLL
                     VIQKAGYLFSVLLLFSNFSVPFIKAKEYELFKNSK"
     gene            986..1225
                     /locus_tag="VWX16_001946"
     CDS             986..1225
                     /locus_tag="VWX16_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159238.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1210..1488
                     /locus_tag="VWX16_001947"
     CDS             1210..1488
                     /locus_tag="VWX16_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159239.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKITSCCIVAKDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            1784..>3137
                     /locus_tag="VWX16_001948"
     CDS             1784..>3137
                     /locus_tag="VWX16_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HEU9159240.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASSPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKYDAAKKDYDEAKKKAAEAQKKYEEDQKKTEEKAKKEKEAAKEVDDASLA
                     VQKAHVEYRKVLDSRNSYRNPSDYAKKLAEADKKITEETTKLTNAQTKFQSIRTTIVV
                     PGQSELAETKKKAEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQDEISTL
                     EQEVATAQHQVDNLKKLLAGVDPDDTEAIEAKLKKGEAELNAKQAELAKKQTELEKLL
                     DSLDPEGKTQDELDKEAAEAELDKKVESLQNKVADLEKEISNLEILLGGADSEDDTAA
                     LQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPAP
                     KPEQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMATGWL
                     Q"
BASE COUNT         1172 a          444 c          610 g          911 t
ORIGIN      
        1 taaaaatttg tgtagatacc acgataaaat ttttattcat cagattattt gacacaagta
       61 ctttaatgaa gaggagaata ttttttcttt tcgcattgga tgttatagct tacttatttt
      121 taggatatct tttagtgatt caaaaagcag gctatttgtt ttcggtttta cttctttttt
      181 ctaatttttc agttccattt atcaaagcaa aggaatacga attatttaaa aatagcaagt
      241 aatagaaaca taggttaaat cttagaacgg cttaaaatca gatatgataa agggaaatag
      301 tcttatctag gtaactaagt gctacaaaca cagtcgagga tcacttgtta ggattacatt
      361 ttaatcatcc ttaataagaa tggagaattc ctatggggat tgtattactc tgaagttttg
      421 aataccttag ttctcgtgat tttgagctat tctagcttta gaatccttca gaatgtaaaa
      481 ttttaagcag tcaaagactt agaatagcct gaaaacattt attttatagc aattctaggc
      541 ttagaattgc tcatatatat gtatttgagc tgctcgatag ttaggacagc tctattctga
      601 gaaatctgag ctatcaatgc ttcagaatag tatttcgcca atggttttta ttgaagaggt
      661 gattgtttta tgatagtcta aaataaaact gatttaatgg agtaaatgga gaatcgttac
      721 atgaaacatt tttttgcagg aattggtgag ataaaacttg ttggctactt attgtatata
      781 tgtgtaggac tagcccctct ttttcatata tatgtcatag gttcggaact gagttttgtg
      841 aaaatagtgt tatctatttt gggagttata tttgtaagta tgttaactat tgctcgaatt
      901 tatcggactt ttctataaag aatagtttgg acttgaactg tatctaaaaa cagaggttgg
      961 tcgattatca acctctttct tgtcgttgaa attacttgat tgtattttgg attatcaaga
     1021 aagattcaat ggaaaaacgt gtcaagtatc aacgaattat aagtatttag agattttcaa
     1081 agtgaatttt tgcttgactg atttacatca tttatttggc ttacacaaaa tcacacgaga
     1141 ttatgctagt caaacaaaac ctgctattca agctggtgtt tttattttag aagattttag
     1201 aaatatccta tgtacaatga tgtaatcgaa aggatatctt tatatgagtt tataggtgat
     1261 attttctatt ctaaaataac aagttgttgc atcgtagcta aggatttatc taaaaatact
     1321 atgaaattgg acgtcatatt ttttgaggat aaaaataaaa gatccgcagt tttaggttta
     1381 cgaagagaca aaagcggagt atttaaacca gttactctac attttacaag cgctaagaaa
     1441 tatgctaaag ttcgtaaaac agatgtgaaa gaaatgaaat ggctataaag attgtccgca
     1501 ggcttaagct tgcgtttttt gttgtttaac gaaattaaaa aacaagctct ctcatcggaa
     1561 atgtttttat aggaatgaag gaagatgatg ctatgtttaa aaagagaatc gaaaagggca
     1621 aaagtagtgt ctttatcttt ttggagtaga agcttatgat atagaaattt gtaataaaaa
     1681 tgtaatataa aacatttgac aaatatttac ggggggagta tacttaatat aagtatagtc
     1741 taaaaatgac tatcaaaaaa gaggtaaata gaggtaaatt tagatgaata agaaaaaaat
     1801 gattttaaca agtctagcca gcgtcgctat cttaggggct ggttttgttg cgtcttcgcc
     1861 tacttttgta agagcagaag aagctccaca agttgtcgaa aaatcttcat tagagaagaa
     1921 atatgaggaa gcaaaagcaa aatatgacgc tgctaagaag gattacgacg aagctaaaaa
     1981 gaaagcagca gaagctcaga aaaaatatga ggaagatcag aagaaaactg aggagaaagc
     2041 gaaaaaagag aaagaagctg ctaaggaagt agatgatgct agtttagcgg tgcaaaaagc
     2101 acatgtagaa tatagaaaag ttctagattc tcgtaatagc tacagaaacc cgagtgatta
     2161 tgctaaaaaa ttagcagaag cagataaaaa aataactgag gagacgacta aactaaccaa
     2221 tgctcagaca aaatttcaat ctattcgaac aacaattgta gttcctggac aaagtgagtt
     2281 agctgagact aagaaaaaag cagaagaagc taaagcagaa gaaaaagtag ctaagagaaa
     2341 atatgattat gcaactctaa aggtagcact agcgaagaaa gaagtagagg ctaaggaact
     2401 tgaaattgaa aaacttcaag atgaaatttc tactttggaa caagaagttg ctactgctca
     2461 acatcaagta gacaatttga aaaaacttct tgctggtgtg gatcctgatg atacagaagc
     2521 tatagaagct aaattaaaaa aaggagaagc tgagctaaac gctaaacaag ctgagttagc
     2581 aaaaaaacaa acagaacttg aaaaacttct tgacagcctt gatcctgaag gtaagactca
     2641 ggatgaatta gataaagaag ctgctgaagc tgagttggat aaaaaagttg aatctcttca
     2701 aaacaaagtt gctgatttag aaaaagaaat tagtaacctt gaaatattac ttggaggggc
     2761 tgattctgaa gatgatactg ctgctcttca aaataaatta gctactaaaa aagctgaatt
     2821 ggaaaaaact caaaaagaat tagatgcagc tcttaatgag ttaggccctg atggagatga
     2881 agaagaaact ccagcgccgg ctcctcaacc agagcaacca gctcctgcac caaaaccaga
     2941 gcaaccagct cctgcaccaa aaccagagca accagctcct gcaccaaaac cagagcaacc
     3001 agctaagccg gagaaaccag ctgaagagcc tactcaacca gaaaaaccag ccactccaaa
     3061 aacaggctgg aaacaagaaa acggtatgtg gtatttctac aatactgatg gttcaatggc
     3121 aacaggttgg ctccaaa
//
LOCUS       DAVKAO010000111         2916 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKAO010000111 DAVKAO010000000
VERSION     DAVKAO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.111"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VWX16_001949"
     CDS             complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VWX16_001949"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9159241.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1420..2196
                     /gene="recX"
                     /locus_tag="VWX16_001950"
     CDS             1420..2196
                     /gene="recX"
                     /locus_tag="VWX16_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9159242.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPSRALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLSQVLARKGYDFSDIASALREYL"
     gene            2285..2818
                     /locus_tag="VWX16_001951"
     CDS             2285..2818
                     /locus_tag="VWX16_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9159243.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          929 a          531 c          527 g          929 t
ORIGIN      
        1 ctttttatgt tttgtcaaca ccttttttaa acttttttta ttaattttac aacagcttca
       61 gttcgagctg tatgtgggaa catatcgacc gactggatat aatgaagatc atagacttct
      121 actaagcgta ccaaatcacg agccaaggtc gaaacattac aagaaatata aaccattttt
      181 tctggtacat aagtaagaat agtatctaat aacttatcat ccagacctgt acgtggtggg
      241 tcaacaatca aagcatctgc tcggtagcct tccttgtacc aacgaggaat aatctcttct
      301 gccgttccag cttcataatg agtattgtca aatcccattc ttttagcatt tcgcttggca
      361 tcttcaatag cttctggaat aatatccata cctctgagtg tttttacttt ctttgcaaag
      421 gcaaatccaa tcgttccaac tccacaataa gcgtcaatca aatggtcttc tttatcaaca
      481 tccagcgctt ttactgcttc gctatagagg acttctgttt gctcaggatt tagttgataa
      541 aaagctcgag gggatagtga aaattcataa ttgagtacac cttcttgaat actctcttgc
      601 ccccagataa tctctgtctt ttcaccatat atctcactgg ttttagctgt atttgtatta
      661 acagctactg tcacaacttc tgggaaatct ttaaccaact cttttaccaa ttgagttaaa
      721 ttaagctggc ggtttgtaac aataataatc tgaacctgtc cggtctttct cgcgcgtcgg
      781 accataatag tacggacacc tagaactttt ctctcatccg tgattggaat ctggtgataa
      841 gtaagtaatt ctgctaagcg attagcaatc acttgggttt ccttatcttg taccaggcag
      901 tctttcaact ctactaaata gtgagagttt tgtgcatata agcccgcctt gacctgattt
      961 ttaaattttc gagtctgaaa ttgtaactta gctctgtaat attttggttc ctgcattcca
     1021 atagttggac gaatttcata attttcatat cctgcaggag caaatttttt cagcgcttga
     1081 tgaagtaagt ccgtcttgaa ctccagctgc ttatcataat gcaggtgcat gatttggcag
     1141 cctccgcatt cattataaat agtacaagat ggcacaattc gaaatttaga cttcttgttg
     1201 accttcagta attttgcttc aacaaagttg cgtctaatag aagtaatctg acaatagata
     1261 tcttcgcctt tgagagctcc tggtacaaag actaatgttt tttggtaaaa gccgattccc
     1321 tcaccgttaa ttcccatgcg cttgattttt aatggtattt tttgtttcac tttcagattc
     1381 atacccctat cttatcacat tttgagttat aatagagcta tgaaaatcac aaaacttgaa
     1441 aagaaaaaaa gactctatct gatggagctt gataatggcg acaaatgcta tatcaccgaa
     1501 gatacaattg ttcgttttat gttatcgaga gataaggtga taagcgaaga ggaattgaaa
     1561 gagattcaag actttgctca attttcttat ggtaagaatc tggccctcta ccacttatcc
     1621 tttaaagcac gcactgaaaa agaagtcaga gaatatctga aaaaatacga tattgataaa
     1681 aacattgttt ctcaagttat tgctaatctt aaagaagata agtggattaa tgatggtcag
     1741 tacgcttatg ctatcatcaa taccaatcaa ctttcaggag acaagggtcc ttatgtgctg
     1801 actcaaaaac tagctcaaaa agggatttca aaatctacta tagaagagaa cttgaaagaa
     1861 tttgattttt ctgaagttgc tcaacgtgta gctaataaac tattgaaaaa atatgaggga
     1921 aaacttccat ctcgtgccct gcaagataag attatccaga acttgactaa caagggcttc
     1981 tcttattctg atgctaaaat tgcctttgac gacttagata gccaagttga ccaagaaacg
     2041 actcaagaac tcatcttcaa ggaacttgat aagcaatata ctaagtatgc tcgaaagtat
     2101 gaaggatacg aactgaagca gcgtttaagt caagttttag cacgaaaggg ctacgatttt
     2161 tcggatatag caagcgctct tagagaatat ctttaatatt tccaagtgaa attcacagat
     2221 tttaggtaat tttatggtac aatagtaaat gataaactta taaattgtag aaagttggtt
     2281 agttatgaag cttccaaaag aaggcgactt tattacaatt caaagttata agcatgatgg
     2341 gagtctccac cgaacttggc gggacaccat ggtactaaaa acaacagaaa acgccattat
     2401 tggtgtcaac gatcatacac ttgttaccga aagtgatggt cgtcgttggg tcactcgaga
     2461 accggctatt gtttactttc acaagaaata ttggtttaat atcattgcca tgattcgtga
     2521 taatggaact tcttactatt gcaatatggc tagcccctac tatctggatg aagaagcact
     2581 gaagtatatt gattacgatt tggatgttaa aatttttaca gatggggaaa aacgtctctt
     2641 ggacgttgaa gagtacgagc gtcacaaacg caaaatgaat tattctgatg acttggacta
     2701 tattttaaaa gaacatgtca aaattcttgt tgattggatt aacaatggac gtggtccttt
     2761 ctcagaagcc tatgtaaaca tttggtacaa gcgctatgta gaactaaaga atcggtaaag
     2821 ttgtcaaagg tcaggaacaa aatcctgacc tttttttggt agtataaaat aaattgtgta
     2881 aacacaaaaa ggaataaatc tattatagca gagtta
//
LOCUS       DAVKAO010000112         2915 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKAO010000112 DAVKAO010000000
VERSION     DAVKAO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.112"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            159..1136
                     /locus_tag="VWX16_001952"
     CDS             159..1136
                     /locus_tag="VWX16_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9159244.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETATTFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQTHHT
                     MTEEVAAFAHMIQQPDLNLYQTWLDDAGSVHELLYTMRQTAGIRFEAEK"
     gene            1133..2215
                     /locus_tag="VWX16_001953"
     CDS             1133..2215
                     /locus_tag="VWX16_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9159245.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFDPLLTGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKNGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(2279..2915)
                     /locus_tag="VWX16_001954"
                     /pseudo
     CDS             complement(2279..2915)
                     /locus_tag="VWX16_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          906 a          647 c          515 g          847 t
ORIGIN      
        1 tttactagat tctctggctc tatctattta ttcggaaaag aaagcttttc ttcagaagaa
       61 agctagtagc ttatttgaaa ttttctcctc tctgttggat agtcccgtct atctatgtta
      121 taatgaaaga aggatttaaa atcggaaaag gagtatttat gctaaaatta ggtgtcatcg
      181 gaactggcgc tatcagccat catttcatag aagcagccca taccagtgga gaataccaac
      241 tggtcgcaat ctattctaga aaactagaaa ctgcaacaac ctttgcttct cgctatcaga
      301 atatccaact attcgatcaa ttagaagtct tcttcaaatc ttcttttgat ttagtttata
      361 tcgccagtcc aaactcctta cattttgctc aggcaaaagc tgccttgtct gctggtaaac
      421 atgttattct tgaaaagcca gctgtcactc aaccacaaga atggtttgat ttgattcaaa
      481 cagctgaaaa aaataactgt tttatctttg aagcagctcg taattaccac gagaaagcct
      541 ttactactat caaaaacttt ttagcagata agcaagtgtt aggagcagat ttcaactatg
      601 ccaaatactc ttccaagatg cctgacttgt tggctggaca aacaccaaat gtcttttcag
      661 accgttttgc tggtggagcc ctcatggatt tgggaattta tcccctctat gctgctgttc
      721 gtctttttgg aaaagctaat gacgcgactt accatgctca acagcttgac aatagcattg
      781 acctaaatgg agatggtatc ctcttctacc cagactatca agttcacatc aaggctggaa
      841 aaaacatcac ttccaatctt ccttgcgaga tttatacaac agatggaacc ttgactctca
      901 acacgattga gcatattcgc tcagctattt ttaccgacca ccaaggaaat caagtccagc
      961 ttcctatcca acagactcat catacgatga ctgaggaagt cgctgcattt gcacacatga
     1021 tccagcaacc agatctgaat ctctaccaaa cttggctgga tgatgcaggc tctgttcatg
     1081 agctactata taccatgcgc cagactgctg gcattagatt tgaggcagaa aaatgaaaac
     1141 caaactaccg actgaatggc aagaactgag tgaccaactc ggtttccaag aattcacccc
     1201 cattcaaact caactatttg atccccttct tactggagaa aacctcctag gagtgagcca
     1261 aaccggaact ggtaagaccc tagcgtatct cctaccaagt cttctcagac tacaaaagaa
     1321 aaaagcccaa caactcttga ttctagcacc aaacacggaa cttgctggac agatttttga
     1381 tgtatgtaaa acgtgggcag aagctatcgg attgactgct caactcttcc tttcaggttc
     1441 aagtcagaaa cgacaaattg aacgccttaa aaacgggcca gaaattctga ttggaactcc
     1501 tggccgtatc tttgaactca ttaaacttaa aaaaatcaag atgatgaatg tggaaaccat
     1561 catcctggat gaatttgacc aattactaga tgattctcag attcactttg tagaaaaaat
     1621 cactcactac gcacctcgtg accaccaact ggtctacatg agtgccacga ccaagtttga
     1681 ccaagaaaag attgtgccga atacacgcac tattgatctc tctgatcaaa aattggacaa
     1741 tatccagcat ttctacatgc aggtagacca acgtcatcga gtggatatgc tacgaaaact
     1801 ggctcatgta gaggatttcc gtggtctagt cttcttcaat agcctgtcag accttgggaa
     1861 tgccgaggaa aaactacagt atcgtgatat attggctgtt tccctcgcta gtgatgttaa
     1921 tgttaaattt agaaaaatca tcttagaaaa gtttaaagat aaccaactaa ccctgcttct
     1981 tgcaactgac cttttggctc gtggaattga tatcgatagc ctagaatgtg tcgtaaactt
     2041 tgatattcct agagatagcg aaacctacac acaccgtgct ggccgtactg gtcgtatggg
     2101 caaggaaggt tatgttatca ctctcgtcac tcatccagaa gaaatcaaaa aacttaaaaa
     2161 gtttgcaagt atccgagaaa ttgtcctaaa aaaccaagaa ctctatatca aataaagccc
     2221 cattgctcta tacacatcca gtgtatggag cttttttata agcaattcaa ttttatactc
     2281 atctgctttc aaaaagcatt ctagtccatc tccgattaac gatggacttt atcacctcct
     2341 tctccaatcc ttatatgaca tcttgaagtt gattcatgac atcttccaaa attcgaaagg
     2401 ctttattctt aaatccacgt ttacgaatct ctttccacac ttgttcaatg gggttcatct
     2461 ctggtgtgta tggaggaata aatgcaaaac caatattagt cggaatcttt aagatacttg
     2521 atttatgcca tatagcattg tccataacga gtaaaaaata atcatctgga taagcttgtg
     2581 aaagctcttc taaaaaggcg ttcatccact cagtattaca tctaccagct attaagaaaa
     2641 atgattcgcc tgtatgggca tcaacagctc cataacaata gcgaaattct cgtatatagt
     2701 gactatggat atgtggacct actcctattg gagcccaaca agatcccagt ttactgattc
     2761 taccgaaacc agcctcatct tggtacatca agcgaacctt acgaaaacgt ctactatatt
     2821 taaaacgctt tcttgccttc ttgaattgag attttatttt tagacgcgac aatggtttga
     2881 gcgtctgctt tcttaggatg ttctggacgt ggcat
//
LOCUS       DAVKAO010000113         2895 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKAO010000113 DAVKAO010000000
VERSION     DAVKAO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.113"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            34..486
                     /locus_tag="VWX16_001955"
     CDS             34..486
                     /locus_tag="VWX16_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159246.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1318..1677
                     /locus_tag="VWX16_001956"
     CDS             1318..1677
                     /locus_tag="VWX16_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159247.1"
                     /translation="MGQEIKLIRKQFRSTRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMG"
     gene            1687..2052
                     /locus_tag="VWX16_001957"
     CDS             1687..2052
                     /locus_tag="VWX16_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9159248.1"
                     /translation="MVYRYRTNLKKVFLTDPELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            2039..>2895
                     /locus_tag="VWX16_001958"
     CDS             2039..>2895
                     /locus_tag="VWX16_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU9159249.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNLDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFTNNDKLYESRNDRQEKHQQTIHAHHIIQSFSPEDNLTPEEINR
                     IGYETMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKMTGAKIIEKRYSYRDYKKYKESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEEFFRTHFAKQEIESRLEFLLNR
                     VNSLEDLIT"
BASE COUNT         1022 a          443 c          580 g          850 t
ORIGIN      
        1 cgaaaaattt tttctaaaga aaatacacga tttttggagt ttaatattaa ctcgaaagaa
       61 tcttcgcaac aacttgatat tctctataaa gaagttggtg tattaagttt gggacaaaaa
      121 gttgtagcca tgcttgattt tttattagca tatagtgact attctaaaga cttcagacca
      181 ttgattattg atcagcctga agacaatcta gacaatcgtt atatttacag gcatttagtt
      241 cagcagttta gagatgtgaa agctcaacgt caaattattt tagcaacaca taatgctaca
      301 attgtaacaa attctatgac agatcaagtt gttattatgg agtcagatgg agttaacgga
      361 tggattgaat cacagggata tgttagtgaa aaatatataa aaaatcatat catcaatcaa
      421 ttagagggag gaaaagattc cttcaagcat aaaatgtcta tatatgagac ggctttatca
      481 gagtagagtc agaaaaagta ggctagaaat ttagcctact tttttctttg tccgacaggc
      541 atagtgtaca tctgaggtcc aagtcctctg tggatatttg ctgcagatga aaccaatagc
      601 gactcctaat cctgaatatc gtgaggtagg ggggatagga aggaattagc gaaatcaagg
      661 ctctacaaac agaatcgtga cttgaagcca tatatagcgg atgaggaact ctaaaatcca
      721 aataggtgtc gtaacctata tacgtgaatt acgagagtaa actaggaaag atgtacggct
      781 tattccgtga gcgtttagga cgtagtacaa cgaatcatgg gagtcagctg aacacatagt
      841 attgaagaaa tttctgtaat ggaaatggag cgaagaagtg aacaattaaa tgaatacctc
      901 tctaattaaa tttgtcaatt ctaattcctg gtatgaaaag acagtgacct gaaaatgtaa
      961 atgatgggag ctgatcataa atataggacg atacatgcag tagtgttaga gattagtccc
     1021 tacttgattt gtgataactt ccccaaattt cttctgctat acttttctca acttttaaaa
     1081 atccaactaa gaattttacc tgggtgtttg ggggcggagc caccaagtta tcttatggtt
     1141 agctgtcaaa actagaaggt tttgataggc tggcgatatg atttttggga tattgtgtcc
     1201 acaatatctg agctcgcaaa gaccgaacaa ggagagactg aggtctttag gaatcgtact
     1261 gacaatgtga ggcagcctta cattgtcaga caagtataat gttgaaagga tggtaagatg
     1321 ggacaagaaa ttaagttaat ccgtaagcaa tttagaagca cgagacaaga agaaaaacag
     1381 ataaaagaaa tgatgaggga acaaaaagtg gatagtttct cagaatttct tcgtcaaaat
     1441 ttattgaaaa agaattatca ggatagaatt tttgaaagtt ggttctccct ttggcagtct
     1501 caaaagtttg aacagattag tcgagatgtg tatgaagttc tggttgtcgc aagagaaaat
     1561 catcaagtga ctcaagagca cgtctcaatc ttattgactt gcgttcaaga attgattgcg
     1621 gaagtcaatc aagtacagcc actcagtcgt gagtttcgtg aaaaatatat gggttaggag
     1681 gatgtaatgg tttatcgcta tcgtaccaat ctcaaaaaag tatttctaac tgatccagaa
     1741 ttacaccaat tgaatgaacg gattgctaag agcaattgtc aaaatttttc agtctatgcc
     1801 agaaaagttt tgcttaatcc caatatgtca tttgtcacga ttaacactga tacttatgac
     1861 cagttagttt ttgaattgag acgtattgga aataacatca atcaaattgc gcgtgcgatt
     1921 aatcaaagcc atctgatttc tcaggaccaa ttacaagaat tgagtaaagg agtcggagag
     1981 ttaattaagg aagtggacaa agaatttcaa gtggaggtga aaagactgaa ggagtttcat
     2041 ggtagttact aaacactttg cgacgcacgg aaaaaaatat cgtaggcgtt tgattaaata
     2101 tatcctcaat ctagataaaa cagataatct aaaattagta tctgattttg gcatgagtaa
     2161 ttacttagat tttcctagcc atgcagaaat ggtagaaatg tacaatgtta actttactaa
     2221 taacgacaag ttgtacgagt ccagaaatga ccgacaagaa aaacaccaac aaactattca
     2281 tgcccatcat atcatccaat cattttctcc tgaagataat ctgacacctg aagaaattaa
     2341 ccgcattggt tatgagacca tgatggaatt gacaggaggt cgttttaagt ttatcgtagc
     2401 aactcacaca gacaaagatc atgttcataa tcacatccta atcaacgcta ttgaccgcaa
     2461 ttcagataaa aagttgatat ggaactatgc cttggaacga aacctccgta tgatttcaga
     2521 ccgtatttct aaaatgactg gtgcaaaaat tattgagaaa cgttattcct atcgagatta
     2581 taaaaaatat aaggaatcta gtcataaatt tgaattgaaa caacgtcttt attttttgct
     2641 tcagcaatct aaatcgtttg atgatttttt agagaaagca aagcagttgc atgttcagat
     2701 tgattttagt caaaagcata gccgtttttt gatgacagat agaacgatga taaaaccaat
     2761 tcgagggcgc caactcagca aacgagatct atatgatgaa gaatttttta gaacgcattt
     2821 tgctaagcaa gagattgaaa gtcgtttaga atttttgtta aaccgtgtca attctttaga
     2881 agatttaata acaaa
//
LOCUS       DAVKAO010000114         2815 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKAO010000114 DAVKAO010000000
VERSION     DAVKAO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.114"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            246..1304
                     /locus_tag="VWX16_001959"
     CDS             246..1304
                     /locus_tag="VWX16_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEU9159250.1"
                     /translation="MLRRNSFLFTATKDGAILRSAKFNYQLNDVSIVIQALKSGDEFT
                     REMDDLDVLLSSVLGISLSYKEIYATHVEYVFVYRQPERLHITSLPLEEDNSLKIKIY
                     DDFIIDLRKNFSMLISGASGAGKSFFTYYYLTRFISQTVNGRHAKIYVIDPKLSDIYK
                     LSKFSGLPVENYGTTNEDAFRIVRHYINEMNRRMEIYNKSDLFDSIGIDLGLPPLLLV
                     IEEYSSLVASMDSKAKKDFENMVAIVAQKARSLSMGVCIVMQQPRSDSLSTNIREQLV
                     NAIFLGAPTRESSQMMFGTTDVPKVKKDKGVGLYSTDREPPKEFHSPMFDRDVFEVIL
                     PVWEWAAKDYMKDEDEDV"
     gene            1656..1919
                     /locus_tag="VWX16_001960"
     CDS             1656..1919
                     /locus_tag="VWX16_001960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159251.1"
                     /translation="MKETVVVLAISTKKERGWIKVSTLNDCWSDLGMHFDKSKFGAVF
                     SAPGLYEVEVINNASFGQNAQYEVIQSRKLGTFAELIEMAKIK"
     gene            2044..>2815
                     /locus_tag="VWX16_001961"
     CDS             2044..>2815
                     /locus_tag="VWX16_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEU9159252.1"
                     /translation="MNKEVLNRAPSNSPITIHQMSNKSYSKFQEEVSLKYGFIGLKLD
                     KLSLTAEVSEEFHSEILSGNFTLYDYFGVVEPNLNRNGELASYKGQFFNDEKENWYIE
                     YTPVASIKMNKRPLKIEFTPSKVSKKNFLIVFHRMFPYMFNIAISTFHLAYDFERDLS
                     ALRVNWPKVMYRPIYKGMKLETMDFGAPKGNYHLTAYNKLKERMDSGDMAEIEIYQQY
                     DNLWRIEYKFYNEGNIKKELKNGLPFLAKIPVYIENFKG"
BASE COUNT          913 a          400 c          562 g          940 t
ORIGIN      
        1 aacaaacggc ttctttgtct gagcactact ttttaaatac atctaaattg agaagtattc
       61 gctggtttac tataggattt ttgagcatat tgtctcttta tagttgtatt ctttttaagg
      121 gatggtttct tcaaatgttt acattatctg ttggattaat tgtaactttg tactttgaaa
      181 gaaaaattaa gggatgtttt catcagattg aaccgctgtt aattgtcaga gaaaaccttc
      241 tatttatgct tcgcagaaat tcctttcttt ttacagcaac aaaagatggc gctattcttc
      301 gttcagctaa atttaattac caattgaatg atgtttccat tgtaattcaa gcattgaaat
      361 ctggagatga atttacgaga gagatggatg atttagatgt tctcttaagt agtgttttag
      421 gaataagctt atcatataaa gaaatttatg ctactcatgt tgaatatgtt tttgtataca
      481 gacagcctga acgtttacat attacctctc tccctctgga agaagataat agtttgaaaa
      541 ttaaaatcta tgatgatttt attatcgatt tgagaaaaaa cttttcaatg cttatctcgg
      601 gcgcgtcagg tgctgggaaa tcatttttta catattatta cttgactcgt ttcatatctc
      661 agaccgtaaa tgggagacat gcaaaaatat atgtgattga tccaaaactt tcagatattt
      721 ataaattatc aaaattttct ggtcttccag tagaaaatta tggaacaact aatgaggatg
      781 cctttagaat cgtgcgtcac tacattaacg agatgaatag gcggatggaa atttataaca
      841 aaagtgattt atttgatagt attggaatag acctcggcct tcctccttta cttttggtaa
      901 tagaagaata tagttcatta gttgcttcga tggatagtaa agctaaaaaa gattttgaaa
      961 atatggttgc tatcgttgct caaaaagcaa ggtctctatc tatgggggtt tgtattgtta
     1021 tgcaacaacc ccgttccgat agtctttcta ccaatattag agaacagctt gtcaatgcta
     1081 tatttcttgg agccccaact cgcgaatcta gccagatgat gtttggcaca acagatgtac
     1141 ccaaagttaa gaaggataaa ggagttgggc tttactctac agatagagag cctcccaagg
     1201 aattccattc cccgatgttt gatagagatg tatttgaggt gatacttcct gtgtgggagt
     1261 gggctgccaa agattatatg aaggatgaag atgaggatgt atgagataga aatacttgtg
     1321 aagctaggag atgtaaatgt aatgaaggat tatgaatctg ataccaatcc atataaggcg
     1381 ttgctaaaag caatgaatat ggctcacatg tttatcgttg acgagctgga atagagggat
     1441 tgaatattat gattttaaaa cacatctgtg gtgtgtgaga atcttaagcg ttttgaagat
     1501 gaataaaatt cttttattca ttttcaaaat gccattgtgc agccggcgaa caacaaggcc
     1561 ggttcgaacg aacgttcgaa taaatgtatt tatgtccaat actgattgac gttaaaagct
     1621 gtatattatt ttaaataagg aaggattttt taactatgaa agaaactgtt gttgttctag
     1681 ctatttctac taagaaagaa cgaggctgga taaaagtctc cacccttaat gattgttgga
     1741 gtgacctagg tatgcatttc gataaatcca aatttggagc tgttttctct gctccaggcc
     1801 tttatgaggt tgaagtgatt aataatgctt catttggaca gaatgcccaa tatgaggtaa
     1861 ttcaatccag aaagcttggg acctttgcag aattgattga aatggcaaaa attaaataat
     1921 ttatatttag ggtaaggctg gacctgccag ctttgctggc aagtggagcg tggtcagtgg
     1981 aacggcagtc ttgccctaaa atttaagtat atagaatgtt ttgttttttg aaggagaaat
     2041 ttcatgaata aagaagtttt aaatcgggct ccttctaata gcccgataac gatacatcaa
     2101 atgtctaata agagttactc taaatttcaa gaggaagtat ctctgaagta tgggtttatc
     2161 ggtctgaaat tggataagct aagtttgacc gctgaagtta gtgaggaatt tcattctgag
     2221 attcttagtg ggaactttac cttatatgat tatttcggtg tagttgagcc gaatttaaat
     2281 agaaatggtg aactagctag ttataaaggg caattcttta atgatgaaaa agagaattgg
     2341 tatatcgagt atacccccgt tgcgtcgatt aagatgaaca aaagaccttt aaagattgaa
     2401 tttactcctt caaaagtttc aaaaaagaat tttttaattg tctttcatag aatgtttccg
     2461 tacatgttta acatcgcaat ctctactttt catttagcct atgattttga gcgagattta
     2521 tctgctttac gtgtaaattg gccaaaagta atgtatcgtc ctatttataa ggggatgaaa
     2581 cttgagacta tggattttgg tgctcctaaa ggtaattatc atctaactgc ttataataaa
     2641 cttaaagaac gtatggatag tggagatatg gcagaaatag aaatttatca gcaatatgat
     2701 aatctatgga ggattgagta taaattctac aatgaaggca acataaaaaa agaattaaaa
     2761 aatggattac cattcttagc taaaattcca gtgtatattg aaaatttcaa aggct
//
LOCUS       DAVKAO010000115         2590 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKAO010000115 DAVKAO010000000
VERSION     DAVKAO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.115"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            220..1422
                     /gene="rpsA"
                     /locus_tag="VWX16_001962"
     CDS             220..1422
                     /gene="rpsA"
                     /locus_tag="VWX16_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9159253.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1507..2590)
                     /locus_tag="VWX16_001963"
                     /pseudo
     CDS             complement(1507..2590)
                     /locus_tag="VWX16_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          792 a          430 c          593 g          775 t
ORIGIN      
        1 gcggtaaggc aagggacttt gactccctca tgcgttggtt cgaatccagc taccccagtt
       61 cttaggtaat aatcaagata gaaagcaaaa tatcttaggg tattttattt ttataattga
      121 aagacgtgaa tgatatgaac atgtccttgc gggtgcttag gaaaaaaatt ataagtatgt
      181 caagtttaag aaaaacttga ttgttggagg attttttaga tgaacgaatt tgaagatttg
      241 ctaaatagcg ttagtcaagt tgagactggt gatgttgtta gtgctgaagt attgacagtt
      301 gatgcgactc aagctaacgt tgcaatctct ggaactggtg ttgaaggtgt cttgactctt
      361 cgcgaattga caaacgatcg tgatgcagat atcaatgact ttgttaaagt aggagaagta
      421 ttggatgttc ttgtacttcg tcaagtagtt ggtaaagata ctgatacagt tacatacctt
      481 gtatctaaaa aacgccttga agctcgcaaa gcatgggaca aacttgttgg tcgcgaagaa
      541 gaagttgtta ctgttaaagg aacgcgtgcc gttaaaggtg gactttcagt agaatttgaa
      601 ggtgttcgtg gatttatccc agcttcaatg ttggatactc gtttcgtacg taacgctgag
      661 cgttttgtag gtcaagaatt tgatactaaa atcaaagaag ttaacgctaa agaaaaccgc
      721 ttcatccttt cacgtcgtga agttgttgaa gcagctactg cagcagctcg cgctgaagta
      781 ttcggtaaat tggctgttgg tgatgttgta actggtaaag ttgctcgtat cacaagcttc
      841 ggcgctttca tcgaccttgg tggtgttgac ggattggttc acttgactga attgtcacat
      901 gaacgtaacg tatcaccaaa atcagttgta actgttggtg aagaaattga agtgaaaatc
      961 cttgatctta acgaagaaga aggacgtgta tcactttcac ttaaagcaac agtaccagga
     1021 ccatgggatg gcgttgagca aaaattggct aaaggtgatg tagtagaagg aacagttaaa
     1081 cgtttgactg acttcggtgc atttgttgaa gtattgccag gtatcgatgg acttgttcac
     1141 gtatcacaaa tttcacacaa acggattgaa aatccaaaag aagctcttaa agttggtcaa
     1201 gaagttcaag ttaaagttct tgaagttaac gcagatgcag aacgcgtgtc actttctatt
     1261 aaagctcttg aagaacgtcc agcccaagaa gaaggacaaa aagaagaaaa acgtgctgct
     1321 cgtccacgtc gtccaagacg tcaagaaaag cgtgatttcg aacttccaga aacacaaaca
     1381 ggattttcaa tggctgattt gtttggtgat atcgaacttt aatcaaattg aaaattcaca
     1441 aaatcctttg tttactaaac aagggatttt tctggctctt tgtcaactgt agtgggttga
     1501 agtcagctaa gctcgagaaa ggacaaattt catcctttct tttttgatat tcagagcgat
     1561 aaaaatccgt tttttgaagt tttcaaagtt ccgaaaacca aaggcattgc gcttgataag
     1621 tttgatgaga ttattggtcg cttccagttt ggcgttagaa tagtgtagtt gaagggcgtt
     1681 gataatcttt tctttatctt tgaggaaggt tttaaagaca gtctgaaaaa taggatgaac
     1741 ctgcttaaga ttgtcctcaa taagtccgaa aaatttctct ggttccttat tctggaagtg
     1801 aaaaagcaag agttgataga gctgatagtg gtgtttcaag tcttccgaat agctcaaaag
     1861 cttgtttaaa atctctttat tggttaagtg catacgaaaa ataggacgat aaaatcgctt
     1921 atcactcagt ttacggctat cctgttgaat gagtttccag tagcgcttga tagccttgta
     1981 ttcatgggat tttcgatgaa actgattcat gatttggaca cgcacacgac tcatagcacg
     2041 gctaagatgt tgtacaatgt gaaagcgatc aagaacgatt ttagcattcg ggagtgaaac
     2101 agcctgggag actgtttcag cctgagccta gaaatttgaa agcgaagctg tttagccaag
     2161 tcatagtaag ggctaaacat atccatagta ataattttga cgcgacatcg gacagctcta
     2221 tcgtagcgaa gaaagtgatt tcgaatgata gcttgtgttc ttccctcaag aacagtgatg
     2281 atattgagat tgttaaaatc ttgcgcaatg aagctcatct ccatctcccg attgaaacag
     2341 tcactccccg gactgtttca acgtcccagg acataatctc aggaagacgc gaaaaatcat
     2401 gctcaaagtg agaatcattg agcttgcgaa tgacagttga agttgagatg gaaagctgat
     2461 gggcaatatc agtcatagaa atcttttcaa ttaacttttg agcaattttt tggttgatga
     2521 tacgagggat ttggtgattt ttctttacca ggggagtctc agcaaccatc atttttgaac
     2581 agtgatagca
//
LOCUS       DAVKAO010000116         2537 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKAO010000116 DAVKAO010000000
VERSION     DAVKAO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.116"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(1..674)
                     /locus_tag="VWX16_001964"
                     /pseudo
     CDS             complement(1..674)
                     /locus_tag="VWX16_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(676..1494)
                     /locus_tag="VWX16_001965"
     CDS             complement(676..1494)
                     /locus_tag="VWX16_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9159254.1"
                     /translation="MVKLWKRYKLFISAGMQELITYRVNFFLYRIGDVMGAFVAFYLW
                     RAVFSSSHQSLIRGFSLSDMTFYIIMSFVTNLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTEIGFRWLVFVSVGLPFLIIIIGLKLLSGQPILQISLMTLTYLLSLILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLSFFPKIIADILYFLPF
                     SSLIYTPVMIIVGKYNISQMIQAVLLQLFWLLVMIALSQIIWKRVQSHITIQGG"
     gene            complement(1496..2488)
                     /locus_tag="VWX16_001966"
     CDS             complement(1496..2488)
                     /locus_tag="VWX16_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9159255.1"
                     /translation="MAMIEVSCLRKDFVKIIKEPGLKGAIRSFIHPEKQIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQENRQDYVKDIGVV
                     FGQRTQLWWDLALQETYSVLKEIYDVPDAVFQKRMDFLNDVLDLKEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVNQLKETFGKMKTLTFELRPGQNHVVSQFVGISDIH
                     VTRKELLLNIQYDSSRYQTADIIQKTLSDFAIRDLKMTDVNIEDIIRRFYRKEL"
BASE COUNT          941 a          485 c          394 g          717 t
ORIGIN      
        1 cttatctttc aagaaatagc tagctgggta gtaggctgta aaagcaaaag gaataataaa
       61 acttatcaac caacgtaaaa atgaattgta gatgacaacc ggatattttg caaaatcatt
      121 gaacatataa aaaatgtaaa tcactgcccc agattgtttg gtccaaaaag ctatgctggc
      181 tgttgcaatt tttaaggaag tataaatcaa ggtggcaaaa ggtatactaa ttaaaaagat
      241 aaaaaccttt gtccaagtcc aagtcaagct ggttaccgtt gctgatagta atgaaatacc
      301 tactaacaat tcacctaaag catcaatttg aaaagtttct actagaatat gaaataaagg
      361 attaatgggt cttgtcaggt atttatcaaa ttcgccctta cgaaccaggc gttgccctag
      421 agcccataaa ttatcaaaaa agagatgatc aattcccttg ggaattaggg aaaagccata
      481 gataaaggca atctgctgaa aactccaccc atcaagtgat gggatgtttt gaaatataat
      541 attaagaaat aacaggttta acccttgcgt taaaaaacgc ctaaaacgcc aacaacgaag
      601 tcaaccttat actccacaat ttgcttgagg tattgtctga taaaaatcaa gtgcatacgt
      661 tgatatttct tcatactaac ctccttgaat agtaatgtgg gactgtacac gtttccaaat
      721 aatttgggac aaagcaatca taacaagcaa ccaaaataat tgtagcaaga cagcttgaat
      781 catttgacta atgttatact ttcctacaat aatcatcact ggcgtataaa ttaaagatga
      841 aaatggtaaa aaatataata tatccgcaat gatttttgga aaaaaagaca aaggaatcaa
      901 actaccagac ataaaggcaa ccaaagaatt ctttagaaga tttgaccccc acaaattttt
      961 aaaaacaaaa gcggaaaacc caaaacaaat attaaagaaa aaattaatca aaaaagccaa
     1021 tattaagcta agaagatagg tcaaggtcat tagagaaatt tgcaagatag gttgtccgga
     1081 taagagtttt aagcctataa ttatgattaa aaacggcaag ccaacactaa caaagactaa
     1141 ccacctaaat ccaatctctg tgaaaagata agaagctgca aaatgaaccg gacgaagcaa
     1201 ccgcataata attgaaccgt cctttacttc ctcgccaatc ataaatgagc tatccgattt
     1261 tgttagcaag ttagtcacaa aactcatgat gatataaaaa gtcatatcag ataagctaaa
     1321 tcctcgaatc aaggattgat gcgacgaact gaaaacggct ctccataagt aaaaagcaac
     1381 aaatgctccc ataacatccc caattcggta aagaaaaaaa ttgacacgat aagtaatcaa
     1441 ttcctgcatt ccagcactaa taaatagttt gtagcgtttc cataatttaa ccatcttaaa
     1501 gctcctttcg gtagaaacga cggataatat cttcaatatt tacatcggtc atctttaaat
     1561 ctcgaatggc aaaatcagac aaggtttttt ggataatatc agctgtttga taacgcgagc
     1621 tatcatattg aatatttagt agcaactctt ttcgcgtaac atgaatatcc gaaatcccta
     1681 caaactgaga gacaacatga ttttgacctg gacgtagttc aaaagtaagg gtcttcatct
     1741 ttccaaaagt ttctttcagt tgatttacag tgccgtcaaa aatctcttgt cccttatcaa
     1801 tcataaaaat acgatcacat aattgttcaa tatcactcaa atcatgagtg gttaagagta
     1861 ttgtcgtctc ttcttcttga ttaatctgtg tgatcgctcg tcgaatatta tccttgacag
     1921 aaacatctaa accaatggtg ggctcatcta aaaaaaggac tttgggatta tgcaataaag
     1981 aagccgcaat atcagctctc atacgttgac ctaacgatag tgtccgaaca ggatccttaa
     2041 taaactcttt caagtctaaa acatcattta aaaaatccat gcgtttttga aatactgcat
     2101 cagggacatc ataaatttct tttaaaactg agtatgtctc ttgtaaagcc aaatcccacc
     2161 aaagctgggt gcgttgccca aaaacaacac caatatcctt aacataatcc tgacgatttt
     2221 cttgaggaat cttcccatta atgcgacaaa aaccagatgt cggttttaaa atccctgtca
     2281 acattttaat cgttgttgac ttacctgccc cattagcacc aataaaacct aaaatttgtc
     2341 cctttggaac ctcaaacgat aaatccttaa ccgcttcaaa aatttgtttt tcgggatgga
     2401 taaaagaacg tatggctcct tttaagccag gttctttaat tatcttaaca aaatctttcc
     2461 gaagacaaga tacttcaatc attgccatac tttactctcc ttttatctat ttatatggta
     2521 atcagtgttc aaatcca
//
LOCUS       DAVKAO010000117         2436 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKAO010000117 DAVKAO010000000
VERSION     DAVKAO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.117"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..871
                     /locus_tag="VWX16_001967"
     CDS             <1..871
                     /locus_tag="VWX16_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9159256.1"
                     /translation="IKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKF
                     DIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQ
                     ILIDGKDIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLAN
                     CTDFIEKMDKGFDTIIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNE
                     KKIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKN
                     LIEKTKMAEEFIY"
     gene            882..1466
                     /locus_tag="VWX16_001968"
     CDS             882..1466
                     /locus_tag="VWX16_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9159257.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1466..2161
                     /locus_tag="VWX16_001969"
     CDS             1466..2161
                     /locus_tag="VWX16_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9159258.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAILCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKIAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2149..>2436
                     /locus_tag="VWX16_001970"
     CDS             2149..>2436
                     /locus_tag="VWX16_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9159259.1"
                     /translation="MVEIKNLSLDYGEKHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNV"
BASE COUNT          875 a          321 c          463 g          777 t
ORIGIN      
        1 gataaaagac tctttagatg catctaaaga gggcttgatg gaaatatttt atttatcgcc
       61 caaaatagaa agattaaaag aaattcaaaa tcaagattta caagaaggcg atgactatag
      121 cttaaaaaaa tttgatattg atctaaaaga tgttgagttt gcctacaata aagacgcaaa
      181 agttttaaat ggtgtaagtt ttaaagctaa gcagggagag gtcactgctt tggtaggtgc
      241 aagtggctgc ggtaaaacaa ctatcttgaa acttatatca agactttatg attatgacaa
      301 gggacaaatc ttaatcgatg gcaaagatat aaaagaaata tcaacagaat ctctttttga
      361 taaggtctct atagttttcc aagatgtggt tctctttaat caaagcatta tggaaaatat
      421 tagaatcgga aagcaagatg caagtgacga agaggttaaa agagcagcaa aacttgcaaa
      481 ttgcacagat tttatagaaa aaatggataa aggtttcgat acaattattg gtgagaacgg
      541 agctgagcta tcaggaggag aaagacaaag attatcaata gccagagcct tcttaaaaga
      601 tgcgccgata ttgatcttag atgagataac agcaagcctt gatgttaaca acgagaaaaa
      661 gattcaagag tctttaaata atttagttaa agataaaact gttgtaatca tttcacatag
      721 aatgaaatcc atagaaaatg cagacaagat agtagttctt caaaacggaa gagtagaaag
      781 cgaaggtaag catgaagagc ttttacaaaa atcaaaaatt tacaaaaatt taatagaaaa
      841 gacaaaaatg gcagaagaat ttatttatta ggaggactac aatggataat aaaaaattaa
      901 aagtaaaaga tttagtaagt atcggtgttt ttggcgtaat ttattttgcc ttcatgtttg
      961 gagttggtat gatgggcttg attccaatat tgttcttaat atacccgaca gtattagcca
     1021 tagttgcagg aactgttgtt atgttattta tggctaaggt tcaaaagcca tgggcactat
     1081 ttatatttgg tatgatatca ccacttgtga tgtttgcagc tggtcatacc tacgtagttg
     1141 tggttttatc acttatagta atgataatag cagaattaat tagaaagatt ggtaattata
     1201 attcatttaa atacaatatg ctttcttatg caatcttcag cacatggata tgtagctctt
     1261 taatgcaaat gcttttagca aaagaaaaat atatggagtg gtctttgatg acgatgggaa
     1321 aagattatgt tgatgtatta gaaaagttaa taacttatcc tcacatggct ttagtagcct
     1381 taggtgcttt cttaggagga attcttggag catatatagg caaggctcta ttgaaaaaac
     1441 actttgaaaa agcaggcatc gtataatgcc tactttttct tcaaatgctc cctttaatcc
     1501 caatccgatc tctaagatat tagtcgtatt tatcacaggc ttaactgtta tgcatagcat
     1561 aaacatccgt tttgagctgg caattctttg tcttattggt attttgcttt atttgaatgg
     1621 atacaaaaaa acacttttca aatggattat attgtgtggg atactttact ccttgcctaa
     1681 ttttatggtg ttatctgaat taaaccctat agttaagatg tttttgagta tacctattgt
     1741 tattagaatg tttattttac catttatggc agcaagcttt atgataaaga cctcggatgt
     1801 aggcgcaata atttcatcga tggataagct taagatttca aagaatgtat ccatacctat
     1861 tgcggttatg tttagattct tcccatcttt taaggaggag aagaaaaaca tcaaaattgc
     1921 tatgagagta agagggataa attttaaaaa cccagtcaaa tatcttgaat atgtttctgt
     1981 accactactc attatatcat ctaatatatc agatgacatt gcaaaagcgg cagaaacaaa
     2041 ggcaatagaa aatccaattg ctaagaccag atacattcgc gtaaagatac agctaattga
     2101 ttttgtttat gttttagcgg ttgctggact tattgtggga ggcttaatat ggttgaaata
     2161 aaaaatttaa gtcttgatta tggtgaaaag catatattag atgatatatc actatccata
     2221 gccgagggag agtgcgtgct atttacagga aaaagtggaa atggtaagtc atctttaata
     2281 aattcaatca atggactagc tgtaaggtat gataacgcaa agacaaaggg cgaaataatt
     2341 attgatggta agaatataaa aaatttggaa ctttatcaaa tctcaatgct tgtttcaact
     2401 gtttttcaaa atcctaagac atattttttt aatgtc
//
LOCUS       DAVKAO010000118         2418 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKAO010000118 DAVKAO010000000
VERSION     DAVKAO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.118"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            4..969
                     /locus_tag="VWX16_001971"
     CDS             4..969
                     /locus_tag="VWX16_001971"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9159260.1"
                     /translation="MAIIKKIVVLYGGLSEEREVSENSAKEISKSLLTLGYDVISIDL
                     SQDCSYEIGEIEKSERGLNKQKVEIGSGIIDVCRKVDIVFLATHGGIGENGKLQAIFD
                     VEKIDYTGNSFLSTAISMDKKLSKIVASSVGIKCASNLMVDRIQANDFPIVIKPIRSG
                     SSKGIKIFQNKKQFDIYYKEHPELGSVFVEKYIKGREFSVGILGETVLPVIEIKVKNG
                     FYDYNNKYTVGAAEEVVPAKISEKLTHTLQKSAYKIHKALGFNVYSRTDFIVDEKGDV
                     YFIESNSLPGMTKTSLLPQEAKAAGIDFPNLCERIIELSREIRSQ"
     gene            1035..2243
                     /locus_tag="VWX16_001972"
     CDS             1035..2243
                     /locus_tag="VWX16_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HEU9159261.1"
                     /translation="MKIINGICRYIFDSKGFATIEVEIFLDSGDTGIGAAPRGSTTGH
                     YDIQYNEYYPRGNNFSPIPDGNIEFFNENILPRIINREVEDIEDITELDKHLFDIPEI
                     ENYGNVAIACSYAVWEAFSKNKKSPLWKLFFEPGSASKGKVKHLVNIIDGKPDSLLAG
                     FEFLLVSEKEITFQSLLEISNIKNELMIKFKNQGFYTSISNQGALIINTDDFYIILDS
                     LLETLKLYKNRYDIGLDLAMTDRYDSSLGIYKVPWCVSQQQTVTEIMDTYCDWGVKYP
                     LVYLEDPFSDEDLDSWRKFQLIKPLKLQVFGDDFYATNLERISQFKDCADGIVIKPNQ
                     VGSVSKTLEVMEYAEKSGISMAFSQRTAETENNIISHLAMSVTSSYLKAGGLDRLDRI
                     AKYNEVLRNG"
     gene            2236..>2418
                     /locus_tag="VWX16_001973"
     CDS             2236..>2418
                     /locus_tag="VWX16_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9159262.1"
                     /translation="MDNGKISTDGALVNNKNIEVNISSASIRYGISVFEAPKMFLLGS
                     DIYIFRFQDYFNRLKTS"
BASE COUNT          874 a          283 c          479 g          782 t
ORIGIN      
        1 tgaatggcta ttattaaaaa aattgttgta ctatatggtg gccttagcga agagagggag
       61 gtgtcagaaa attctgcaaa ggaaattagt aaaagtctat taacattggg gtatgatgtg
      121 atttctatag atttatctca agactgttcg tacgagatag gagaaattga aaaaagtgag
      181 cgcggattaa ataaacaaaa agttgagatt ggttcgggta ttattgatgt ttgtagaaag
      241 gttgatatag ttttcttagc aactcatggt ggaataggtg agaatgggaa actacaggct
      301 atttttgatg tagaaaaaat agactataca ggaaattcgt ttcttagtac agctattagc
      361 atggataaaa aattatcaaa aatagtggct agttctgttg gaataaaatg tgcttcgaac
      421 ttgatggttg atagaattca ggcaaatgat tttcctattg ttataaagcc aattagaagt
      481 ggttcaagta aaggtattaa gatatttcaa aataaaaaac aatttgacat ttattataaa
      541 gagcatcctg aacttggttc agtctttgta gaaaagtata ttaaggggag agaattttcg
      601 gtaggtattt taggtgaaac tgttttaccg gttattgaaa taaaagtaaa aaatggtttt
      661 tatgattata ataacaagta cactgttggt gcagctgaag aagtcgttcc cgcaaaaata
      721 tctgaaaagt taactcatac cttacaaaaa tccgcatata aaatacataa ggctcttgga
      781 tttaatgttt attcacgtac tgattttatt gtagatgaga agggggatgt atattttatt
      841 gaatcaaata gtttaccagg tatgactaaa acaagtttat taccacagga agcgaaagca
      901 gctggtatag atttcccaaa cctatgtgaa cgtattattg agctgagtag ggaaattaga
      961 tctcaataga aaataaaatc gacgacattt ataaaaaaag taattttcaa tgataaaaaa
     1021 gaaagtggaa gcctatgaaa ataattaatg gtatttgcag atatatcttt gactcaaaag
     1081 ggttcgcaac aattgaagta gagatttttc ttgatagtgg agatactgga attggtgctg
     1141 ctccacgagg gtctacaact gggcattatg atattcaata taatgagtat tatcctagag
     1201 gaaacaactt tagtccaata ccagatggaa atatagaatt tttcaatgaa aacatccttc
     1261 cccgaattat taatagggaa gttgaagata ttgaagatat tacagaattg gataagcact
     1321 tatttgatat tcctgaaata gaaaattatg gtaatgtagc aatagcttgt tcctatgctg
     1381 tttgggaagc gttttctaaa aataagaaat cacctttatg gaaattattt tttgaaccag
     1441 gttctgcatc aaaaggaaag gtgaaacatt tagtcaatat aatagatgga aagccagata
     1501 gcttacttgc aggttttgaa tttcttttag tatctgaaaa agaaattact tttcaatcat
     1561 tattagaaat atctaacata aaaaatgagt taatgataaa gtttaaaaac caaggtttct
     1621 acacatctat ttctaatcaa ggtgcattaa taattaacac ggatgatttt tatattattt
     1681 tggattcgtt attagagaca ttaaaattat acaaaaatag atatgatatc ggtttagatt
     1741 tagcaatgac agatcgttac gattcttcac ttggcatata taaagtacca tggtgtgtta
     1801 gtcagcaaca gacagtcaca gaaattatgg atacttattg tgattgggga gttaagtatc
     1861 cattggttta tcttgaagat ccattttccg atgaagattt ggatagttgg agaaaatttc
     1921 aattgataaa acctctaaag ttacaagttt ttggtgatga tttttacgca acaaacttag
     1981 aaagaatttc acagtttaaa gattgtgctg atggtattgt aataaagcct aatcaagttg
     2041 gctcagtgag taaaacactt gaggtgatgg aatatgcgga aaaatcaggg attagtatgg
     2101 ctttttcaca acgcactgct gagactgaga acaatattat atcacattta gcaatgagtg
     2161 tgacttcttc ttatttaaaa gctggaggtc tggacaggtt ggatagaatt gcaaaatata
     2221 atgaggtatt aagaaatgga taatggaaaa atcagtacag atggggcact tgttaataac
     2281 aaaaatatag aggttaatat aagctcagct tccattagat atggaattag tgtctttgaa
     2341 gcacccaaaa tgttcttact tggttcagat atctatattt tccgatttca ggattatttt
     2401 aatcgtttga aaactagt
//
LOCUS       DAVKAO010000119         2388 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKAO010000119 DAVKAO010000000
VERSION     DAVKAO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.119"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(37..750)
                     /locus_tag="VWX16_001974"
     CDS             complement(37..750)
                     /locus_tag="VWX16_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9159263.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1209..1577)
                     /locus_tag="VWX16_001975"
     CDS             complement(1209..1577)
                     /locus_tag="VWX16_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU9159264.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAIQIQLAELEINK"
     gene            complement(1999..>2388)
                     /locus_tag="VWX16_001976"
     CDS             complement(1999..>2388)
                     /locus_tag="VWX16_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9159265.1"
                     /translation="FGNASDHVQRNKNGQADTNQTEKPQTEKPEEETPREEKPQSEKP
                     ESPKPTEEPEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKN
                     NLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          713 a          477 c          392 g          806 t
ORIGIN      
        1 atattagctt tttttgtagt cattagctaa tctcctttaa aacgtgatct tttcaagaat
       61 atttttatct aaacaatcca gcaagtcttg gtaagaatag acttcgtaag tcggctgggc
      121 ttgtgtgtga ttttcgaggt gatgaggatt ataccagata gtgtcaatcc ccgcattatt
      181 gccaccttga atgtcggcgg ttagagaatc tccaatcatc agcgtctttt ctttactaaa
      241 tccagcaatt tgctggccaa tcttttcata aaaaagagca tccggctttt gagtttgcaa
      301 ctgttctgag ataaagactt gattgaaata aggtgctaga ccagattgag ccaaacgtcc
      361 tgtctgaatg gcagtaatgc catttgtcgc agcatacaag ttataatcac gctcaatgag
      421 gctgtccaag agatcatgag cgcccgatag tgtttgtccc tgctgggcga ggtaaaattg
      481 gtaacgctgg gcaagaaaac taccgtcttt ttcctgtcca aaatgagcaa ataaacgaga
      541 aaagcgcgtg ttaaccagct cttgtttact gattttcttc agctccaagt ctttccagag
      601 agccttgttc ataggaacgt aataatcttt ataagcctga atatccgcaa ctccttcttc
      661 ttttagaagt tgagtcaaag ccacatcctc agcagcatca aaatcaagaa gagtgtggtc
      721 gaggtcgaag agtagaaatt tgtagaacaa tttgaggttt tcctttctga aaattcatta
      781 agaacattat atcataaagc acctcataca attaactaat ttaatcactt aaaaaaaatt
      841 cgaacacttt ctatacaact gacagctcaa atctttcaga atagaacaat actaactatc
      901 gaacaccccg tcttcataaa tacatatgta attctaggcc tagaattcct ataaactaaa
      961 tgctttcata ctcttccaag taattgattg ccttaaattt taatttttga aggtttctaa
     1021 agctagaata gccccatcac aatcagtttt gattgattca caatttagaa acactatagt
     1081 ttcactcctg ttaaaataaa aaggaactgc ataaagcaat ccctttctga ttttgaaatc
     1141 atttacttaa cattttatag ttgagataat caatagctta tctataaaaa gagttatagt
     1201 aaaattcctt atttattgat ttcaagctcc gctaactgta tttgaattgc accagcctga
     1261 agaagagcag ctgcattata ggcaccttct acaattggaa ccctgttgat gatgatactt
     1321 ttatcactga aatcagtcac catttttaag ttcattttag cagaacctag gtcaaaaaag
     1381 gcaagtaaag tatctgctgg attttcggaa acaaccctat ctacttgatc aaaactcgtt
     1441 ccaattcctc cgccctcggt tcctcctaca taagtaatcg gaacatcttt agctacttta
     1501 ctaatcagtt caacaacacc ttctgcaatg tgtttggaat gtgaaacgat aacaagacca
     1561 ataccaatac tttccatcaa accactccag tttctaaaat agcagtaaag agtaatcctg
     1621 atgagaatga tccaggatca atatgtccaa gaaaccacat gctcctaaga caagagctaa
     1681 cagactggcc atcaataata gtattgttct ttttttcatc attactcctt aactagtgtt
     1741 taactgatta attagccagt aaatagttta tctttattta cactatctgt taagatatag
     1801 taaaatgaaa taagaacagg acagtcaaat cgatttctaa caatgtttta gaagtagagg
     1861 tatactattc tagtttcaat ctactatatt ttgcacattt tcatgaaaaa aatgagaact
     1921 agaactcaca ttctgctctc atttttcgtt ttcccgttct cctatcctgt ttttaggagt
     1981 tagaaaatgc tgctaccttt acttactctc ctttaataaa gccaatagtt tttcagcttc
     2041 tgccataata gtattgttgt cctgggtgcc aaatagtaaa ttatttttta atcctgtgag
     2101 agtctctttg gcattggact tgataattgg atcctggatt tttccaagta aatcttcagc
     2161 ctctctcagt ttttcttcaa ccttttcagt ctcgacctga ggttcttctg attcctctgg
     2221 tgattcttct ggttcctctg ttggttttgg agactctggt ttctcgcttt gcggtttctc
     2281 ttctcgaggg gtttcttcct caggtttttc tgtctgaggt ttctccgttt gattggtatc
     2341 agcttgacca tttttgtttc tttgaacatg gtcgctagcg ttaccaaa
//
LOCUS       DAVKAO010000120         2281 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKAO010000120 DAVKAO010000000
VERSION     DAVKAO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.120"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            88..1611
                     /locus_tag="VWX16_001977"
     CDS             88..1611
                     /locus_tag="VWX16_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9159266.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNEGSIKIGGIAISEYSDEALIKAI
                     SFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1595..>2281
                     /locus_tag="VWX16_001978"
     CDS             1595..>2281
                     /locus_tag="VWX16_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9159267.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     EDLYKKMEDSE"
BASE COUNT          816 a          308 c          429 g          728 t
ORIGIN      
        1 aattcaaact tatccggtgc agagagtata gcattaaaat ctgttattac actaacaagt
       61 ggagcgatat tttattttgt ctcaggaatg ttttcacata tcttgggatt taggcttgaa
      121 acaaatttaa gaaaaagggg aatcgatggt ctggaaaaag caagttttag gttctttgac
      181 ttaaatccat ctggtcaaat aagaaagatc atagatgata atgcagcaca aactcatcag
      241 gtggtagctc acatgattcc agatagctct caggcaatag tcacacctat acttgtcctt
      301 gtactaggct ttatagtaag tataagagtt ggcataattt tgcttgctct tactataatt
      361 ggtggcttaa ttttaggggc aatgatgggc gagcaagaat ttatgaagat ataccaagaa
      421 tccctatcta aactaagtgc tgaaactgtt gagtacgtga gaggaatgca agttgtaaaa
      481 atatttaaag caaatgtaga gtcatttaaa agcttttaca aggctataaa agattactca
      541 aagtatgctt atgattattc cctatcttgt aaaaggcctt atgttttgta tcaatggtta
      601 ttttttggac tgattgcaat tttaattatt cctatagttt attttatgac tagcttagct
      661 agcgcaaagg tgattttact tgagcttatc atgattttat ttttatcagg agttctcttt
      721 gtttcattca tgagaatgat gtggtactcc atgtatattt ctcaaggaaa ttatgcagta
      781 gatactttag aggcgcttta cgaagatatg caaaaagaca aattagtgca tggtaatgtc
      841 aataatttta aaaactataa tatagaattt gagaatgtta gctttgctta taatgataaa
      901 gctgtcattg aaaatttatc ctttaattta gaagaaggaa agtcctacgc acttgtcggt
      961 tcatctggat caggcaaatc aacagtagca aaacttatat caggttttta caatgttaat
     1021 gaaggaagca taaagatagg cgggatagca ataagtgaat attctgacga agccttaatt
     1081 aaagccattt cctttgtttt tcaagattca aaactattca agaagagcat ttatgataat
     1141 gtagctttag ctaataaaga tgcgacgaaa gatgacgtta tgagagcctt aaaattagca
     1201 ggatgcgatt taatattaga caaattccca gaaagagaaa atacaatcat aggctcaaaa
     1261 ggtgtttatt tatccggtgg agaaaaacaa agaattgcaa ttgctagagc aattttaaag
     1321 gattccaaaa ttattattat ggatgaagca tcagcatcta ttgacccaga taatgagttt
     1381 gaattgcaaa aagcttttaa aaatcttatg aaggataaaa cagttatcat gattgcacac
     1441 aggctatcta caattaaaga ccttgatgaa attattgtca tggatagtgg aaaaattata
     1501 gaaagagggt ctgacaaaga attaatgtca aaagatacaa ggtataagag cctgcaagag
     1561 atgtttaaca gtgcgaatga atggagggtt tcaaatgaaa gagttttata aaaaaagatt
     1621 tgctcttaca gatggaggag caagaaattt aagtaaagca acactggctt catttttcgt
     1681 ttattgtata aacatgcttc ctgccatatt acttatgatt tttgctcagg aagttttgga
     1741 aaatatgggc aaaagcaatg gcttttatat agtattctca gttttgactt tgatagcaat
     1801 gtatattttg ctttctatcg aatacgataa attatataac acaacctatc aagaaagtgc
     1861 agatttaaga ataaggacag cggagaattt atcaaaatta cctctatctt acttttctaa
     1921 acatgacatt tccgacattt cacaaacaat catggctgat attgaaggca tagagcatgc
     1981 aatgagccac tcaataccaa aggtgggcgg catggtactg tttttcccat taatatctgt
     2041 aatgatgcta gcgggcaatg tcaagatggg tttagctgta attattccat ctattttaag
     2101 ctttatattt atacctttat ctaaaaaata tcaggttaat ggacagaata gatattatga
     2161 tgtcttaaga aaaaactcag aaagctttca agaaaatatc gaaatgcaaa tggagattaa
     2221 agcatataat ttatcgaagg atattaaaga agacttatat aaaaaaatgg aagatagtga
     2281 g
//
LOCUS       DAVKAO010000121         2107 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKAO010000121 DAVKAO010000000
VERSION     DAVKAO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.121"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(68..946)
                     /locus_tag="VWX16_001979"
                     /pseudo
     CDS             complement(68..946)
                     /locus_tag="VWX16_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(1216..1362)
                     /locus_tag="VWX16_001980"
     CDS             complement(1216..1362)
                     /locus_tag="VWX16_001980"
                     /inference="COORDINATES: protein motif:HMM:NF012457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9159268.1"
                     /translation="MRVKRDYPNYKIYITENGLGYKDEFVDNTVYDDGRIDYDYVKKH
                     LEVI"
     gene            complement(1366..1590)
                     /locus_tag="VWX16_001981"
     CDS             complement(1366..1590)
                     /locus_tag="VWX16_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9159269.1"
                     /translation="MLEVAKALNDFLGISYYMSDWMQAFDGSTEFIHNGKGEKDSSKY
                     QINGAGRCEVPVYVPKTDWDWIIYPQGIYD"
     gene            complement(1882..2070)
                     /locus_tag="VWX16_001982"
                     /pseudo
     CDS             complement(1882..2070)
                     /locus_tag="VWX16_001982"
                     /inference="COORDINATES: protein motif:HMM:NF012457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
BASE COUNT          704 a          404 c          282 g          717 t
ORIGIN      
        1 attttatatt ggatttttgt aaatttctaa agtccctatc tttagattcc gttcagctaa
       61 ctttaattta caaaatagta atatcgaatg atttaatgct agtttgactc ggttctacaa
      121 atgtaacccc tgcttctctt ccaatatatc tgattcctct aatgatgttg ataatccact
      181 ccatagctct aaggctataa atggactttg attagtggta aaccaaataa ttaaatgtgg
      241 aaaatcttta aaatccacct taattccctt attacttttt tagatttcga actgacacta
      301 catgacttta gttgatctag ggtaatagca tcgtgtgaaa acaaagaata atccaaatct
      361 aattcatttt gattttcaag aaaaggggtt ctatcttgta aatcaagtaa tccagtatct
      421 gggaaactct ttggaatgct acatatttaa tattcagaaa attctaaatg ataatcttcg
      481 tatttatcgt tattttctag agggcaatta aatcctgggt gacctccata aagtaaggaa
      541 tagaacgttc aacttcacta aacgttacct gatattccgt agtttttgta gagtcataca
      601 agcgataaat tatattcaaa actaagtgat atggaaattc ttgaagagtc tacacgttag
      661 atttatagct aaaagaaatt gacgattcac ctatcttctt atatgtaaaa ttttattttc
      721 ttactaatcc acgtacagga atattcctat attccccttc tacgagaact taatcatctc
      781 ttacacttac acaaattgga aatagggctt gtgcttggtc actccaatat tctggattac
      841 cttgccatag gtattctaca ccatccttat ctttaataga agataaggca ccgtcaaact
      901 acgaaaactg cactatcaat cgctcatttt ttaattcaat aactatttga tttttcctcc
      961 ccgataccaa acaagagcgt ctgtcttaaa cccacactct tatcttgtgt tagaaattat
     1021 atatactatt caataagttg agattctgct acttggttgt actagtaggc actctttctt
     1081 agagtaatat tcttgtgtct caaagtccag atagaagaga ctataacatt tttcttagcc
     1141 atttgaacat aagaaaacat ccatcaacga ccaaataaac tcccctttaa tatttgtacc
     1201 atctgcaatc gcatcttaaa tcacttctaa gtgtttcttc acataatcat aatcgatacg
     1261 accatcatca taaacagtgt tatcaacaaa ttcatcttta tatcccaaac cattttcagt
     1321 gatataaatt ttataatttg gataatcccg ttttacacgc atgatttaat catatatacc
     1381 ttgaggataa ataatccagt cccaatctgt cttaggtaca taaactggta cttcacaacg
     1441 tccagctcca ttaatttgat atttagagct atctttctca cctttaccgt tatgaataaa
     1501 ttctgttgat ccatcaaagg cttgcatcca gtcactcatg taatagctaa taccaaggaa
     1561 gtcattcaaa gctttagcta cttctaacac ttaacaattt ctctgagtaa ccacctaaat
     1621 aagttgcatc aaaaatgaat ttattatgga tgaggtcttc aagttctact gcacgaacat
     1681 catcagggtt atttttatca aatggttatt ttgtctgaag agcatgaacc acactaatct
     1741 cacccttgtg actaccacct ttaaacagtt taacagcacg agtttaggaa atcatcatgc
     1801 tatggtgtga ttgaaaaact tttacaagat catatttgat acctgatgga aatttactaa
     1861 caagatattg tccatcaccg attagaccaa tttcattgaa agttgtctaa taattaactt
     1921 ctgtaaattc tttaaaacag aactcagcat agtgaacaac gtggccaata tttcccaatt
     1981 aaggaaatca ccgttagaat gcaatgcttc gggagtatca aaatgatgaa ggattacaaa
     2041 taattcaacg ccacgcttat aacactctga aaaaaattat gataaaattc aactcctttt
     2101 cattgac
//
LOCUS       DAVKAO010000122         1983 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKAO010000122 DAVKAO010000000
VERSION     DAVKAO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.122"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(296..607)
                     /locus_tag="VWX16_001983"
     CDS             complement(296..607)
                     /locus_tag="VWX16_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159270.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(622..1908)
                     /locus_tag="VWX16_001984"
     CDS             complement(622..1908)
                     /locus_tag="VWX16_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9159271.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDRVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          584 a          435 c          357 g          607 t
ORIGIN      
        1 ttccaacggc atgactgaga acatccacca cggttggcat cacgcatact ctagacggat
       61 tgttttgaca attctaatct tttagacttt gcataataac tgttaaacag ttgaaaatta
      121 acccagtttt aacaagtagt caaaatacca gataaaggaa aaaagtcttt aaaatcttaa
      181 aaacgcatag tatcaggtat tgaacaatct tgatattatg cgttttattg tgggaagatt
      241 tacttcattt tctcttggaa ttgagttttc tcctacttca aaactagatg atttgctatt
      301 taaaatagcg ttccttatga ttttggcaac ctggattaaa gcgagaaaaa caaaaggggc
      361 aacttaaact ttctttatat tgcttatatg ttagtagttt tagacaaaca ccacataaaa
      421 taggcttatc ctgtatcaaa gataagggat acggctcaaa agggtgattt tctaatgaat
      481 catgacaccg ataacaagca tagtactttt tacaatcata gcattgtaaa gcaataatat
      541 ccagcttgct atggtaatga atgcatcttg tttgcttatc cactaagaaa ccctgtgcct
      601 ctatcatttc cttttccttt cttaagctcg aactgtgacg ctggttccat cttccttata
      661 aagattgatg agtccttctt ttaatgcacg aaccatgtct cctgattgaa cgggttgtgg
      721 gactttaata gtcaatagtt ccattggatt tggagcgcgg tcgattttat tgcctttggc
      781 atcatgcaaa tcttcaatat aggtttcaaa atgacggaaa cctggaccat aaaattcaac
      841 tcgatctcct tcgttaatga cgtttcgttg acgaatggtt gctgtttgtg ccgcatcatc
      901 ataagaaacc acttcagcga caaacttgta ctcaggaatt ttacggcgag caccaaacaa
      961 ctgctcattt tcagatggtg taccatagta aaatcctgta gccagttcac gttgggcaac
     1021 cttccacatc tcgtccacca agtcttgttt gatagcttca aacttttcag gactttcaag
     1081 ataggcatcc acagccgcct tgtagcagtt ggttactgtt gatacgtagt gaatagactt
     1141 catacgtcct tcgattttta gactgtccac accattttca atcatatctg gaatgtggtc
     1201 aatcatagac atgtcaacgg ctgacattga aaattcttct ggaatctcac cctgcaaact
     1261 cttacgttct ttcccaaatg gcatatcgta aaggtcgtat ttccaacggc atgactgaga
     1321 acatccacca cggttggcat cacgcatact catgtggttt gaaagagtac aacgtccaga
     1381 gtatgaaata cacatagctc catggacaaa ggcttcaatt tcaacatctg tacgtttgcg
     1441 gatttcagct aattcttcca ttgaaacctc acgcgctaaa acgacacgag tcaagcctag
     1501 ctctttccag aactcaaggg tttcatagtt agtggcactg gcttgggtag aaaggtggat
     1561 ttcaaggcct ggtgcttcag tagctgcaat cataatcaag gctgggtcag atacgataac
     1621 tgctgcaatc ccgatatcac gcagtttacg gaaccactca ccagcaccag cttcatttcc
     1681 ttcgtgcata accatattag ccgctacata gaccttggca ccatacttgg ccgcaaactg
     1741 cacgccttct tccatctgtt cgaaagtaaa gtttcccgca cggctacgaa gaccataggc
     1801 ctgaccaccg ataaagacag catctgctcc atactgaaca gctaccttta gcttctctaa
     1861 agtccctgca ggtgataaaa cctcaggacg ttttaatgtt tttgtcataa tttctcctat
     1921 ttgcaatata aaagtttgac atttttcctt ctcattttat agtaaataaa ggataaaatc
     1981 aag
//
LOCUS       DAVKAO010000123         1816 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKAO010000123 DAVKAO010000000
VERSION     DAVKAO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.123"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            30..1646
                     /locus_tag="VWX16_001985"
     CDS             30..1646
                     /locus_tag="VWX16_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9159272.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          644 a          212 c          332 g          628 t
ORIGIN      
        1 aattaaaatt tagaaagtga gaaactattg tgtatactat tataaaatca aatataaaaa
       61 aatttagttt attaacgata tttattgttg ctggtcaatt attgctaatt tatgcagcaa
      121 ctattaatgc tctggtgttg aatgaattaa ttgcgatgaa tttagagcgg tttttgaaat
      181 tgtcaatcta ccaaatgatt gtctggtgtg ggataatatt ccttgactgg gtagtgaaaa
      241 attatcaggt tgaagtgatc caagagttta atctagagat tcgaaataga gttgccacag
      301 acatctctaa ctctacctat caagaatttc atagtaaatc atcaggaaca tatctttcgt
      361 ggctaaataa tgatgttcag actttaaatg atcaggcttt taaacaactt tttttagtaa
      421 taaaaggaat ttctggtact atatttgcag ttgtgactct taatcactat cattggtcat
      481 tgactgtagc caccttgttt tcattaatga ttatgctact tgtaccaaaa atctttgcat
      541 cgaaaatgcg agaagttagt ctaaatttaa ctaaccaaaa tgaagctttt ttaaaatcta
      601 gtgagactat attgaatgga tttgatgtgt tagcgtcctt gaatctttta tatgtattgc
      661 ctaagaaaat taaagaagca ggaattttat taaagatggt tatacaaaga aagacaactg
      721 tagaaacgtt agcaggcgct attagcttct ttctcaatat tttttttcag atatctctcg
      781 tttttttaac aggctatctt gcaataaaag gaatagtgaa aattggtact attgaagcaa
      841 taggagcact aacaggtgtt atttttacag cgctaggtga attaggaggt caattatcct
      901 ctattattgg tacgaagcct atttttttaa aattgtattc aattaatcca attgagtcaa
      961 ataaaatgaa tgatatcgaa ccaaatgagg tgaatagaga ttttccgtta tatgaagcaa
     1021 aaaatatttg ctataagtat ggagataaag aaatattaaa aaacttaaat ttttgttttc
     1081 aacgtaatga aaagtattta attttaggtg aaagtggaag cgggaaatct acattattaa
     1141 aattattgaa tggctttttg agagattata gtggagaatt gcgattctgc ggggatgata
     1201 taaaaaaaac ctcctattta aatatggttt cgaatgttct atatgtagat caaaaagctt
     1261 atttgtttga aggtacgatt agagataata ttttattgga agaaaattat actgatgaag
     1321 aaatactaca gtctttagag caagttggtt tgagtgtaaa agattttcct aataacattt
     1381 tagattatta tgttggtgat gatgggagat tactgtcagg agggcagaaa caaaaaatta
     1441 ctttagctag agggctaatt agaaataaga aaatagtatt aattgacgag ggaacttctg
     1501 ctatcgatag gagaacttcg ttagcgattg aacgtaagat attagataga gaggatttga
     1561 ctgtcattat tgttacccat gctccgcatc cggaacttaa acaatatttt actaagatat
     1621 atcaatttcc aaaggatttt atttaagaat ataggggagg gaaacatgag attctattgt
     1681 gaagcttatg aggtaattac tgaagagaaa aaagtatcat ttttttatgt agatgagaaa
     1741 cccttaaggt atctgttatt tgactatata tttctactag ttctagctaa aaaaaaatat
     1801 cccaatctta tgaact
//
LOCUS       DAVKAO010000124         1759 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKAO010000124 DAVKAO010000000
VERSION     DAVKAO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.124"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            336..812
                     /locus_tag="VWX16_001986"
     CDS             336..812
                     /locus_tag="VWX16_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9159273.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            812..1573
                     /locus_tag="VWX16_001987"
     CDS             812..1573
                     /locus_tag="VWX16_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9159274.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFVGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLRVGEKRLNELLEK"
BASE COUNT          649 a          233 c          341 g          536 t
ORIGIN      
        1 gatacgcttt gctaatagcc taataatcac atgtggagtg ttgctacaac gaaaaaggtg
       61 ataatccttg atttcaagct attttataag cattttgtct ttgtagataa aggcaatttt
      121 gacaataaaa atcctaaaag gtgaatcgtt atagatgtat ttgtagatat cgtttgcgca
      181 tcgaaaaaat taatacaata ataaatattt atggctcttt aggtgacttt tatagaagta
      241 aagtttagga tagaaaaaca agaaataatg caccattttt ggtgcgttat gcttttttat
      301 gctataatgg atttataaaa ataaaggagt ttgccatgat tggaaagaac ataaaatcct
      361 tacgtaaaac acatgactta acacaacacg aatttgcacg gattataggt atttcacgaa
      421 atagtctgag tcgttatgaa aatggaacga gttcagtctc taccgaatta atagacatca
      481 tttgtcagaa gtttaatgta tcttatgtcg atattgtagg agaagataaa atgctcaatc
      541 ctgttgaaga ttatgaattg actttgaaaa ttgaaattgt gaaagaaaga ggtgctaatc
      601 tattatctcg actctatcgt tatcaagata gtcagggaat tagcattgat gatgaatcta
      661 atccttggat tttaatgagt gatgatctat ctgacttgat tcagacaaat atctatttag
      721 tagaaaattt tgatgaaata gagagatata gcggctattt ggatggaatt gaacgtatgt
      781 tagagatatc tgaaaagcgg atggtagctt aatggaaatc caagattata ctgatagtga
      841 attcaaacat gctttagcaa ggaatcttcg ttcactgaca agaggaaaaa agtccagtaa
      901 acaacctata gcgattttgc ttggaggtca aagtggtgcc ggtaagacta caattcatcg
      961 tattaaacag aaagaatttc aaggaaatat tgttatcata gatggtgata gttttcgttc
     1021 tcagcatcca cactatttag aactgcagca agaatatggc aaagacagtg tagaatatac
     1081 caaagatttt gtaggaaaaa tggtagagtc tttagtaaca aaattgagta gtttgagata
     1141 caatcttttg atcgagggaa ctttacgaac agttgatgtt ccaaagaaaa cagcacaact
     1201 cttgaaaaat aagggatatg aagtacaatt ggccttaatt gcgacaaagc ctgaattgtc
     1261 gtatctaagt actcttatcc gttatgaaga actgtacatt atcaatccaa atcaagcacg
     1321 cgcaactcca aaagaacatc atgatttcat tgtaaatcat ctagttgata atacacgaca
     1381 attggaagaa ctagctattt ttgaaagaat tcaaatttac caacgagata gaagttgtgt
     1441 atatgattca aaagaaaata caacttcagc agcagatgtt cttcaagagt tactctttgg
     1501 ggagtggagt caggtagaga aggagatgtt gagggtaggg gaaaagagac ttaatgaatt
     1561 acttgaaaaa taaacaattg atatttttag gagaatagaa atgagagggt ttaataacaa
     1621 gataaagtct gtttatcaag aactaacaaa ttccaaagag aaattcggta gctttcacaa
     1681 gactttaatt catttgcata cacctgtttc ttatgattac aagctatttt ctaattggac
     1741 tgcaacgaaa tatagaaaa
//
LOCUS       DAVKAO010000125         1742 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKAO010000125 DAVKAO010000000
VERSION     DAVKAO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.125"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            1..734
                     /locus_tag="VWX16_001988"
                     /pseudo
     CDS             1..734
                     /locus_tag="VWX16_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(850..1653)
                     /locus_tag="VWX16_001989"
     CDS             complement(850..1653)
                     /locus_tag="VWX16_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9159275.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          594 a          334 c          312 g          502 t
ORIGIN      
        1 cagataagga ccaagagctt aaagctgaaa ttcaatccat ctttatcgaa cacaagggaa
       61 attatgctta tcgctgtatt catttagaac taagaaatcg tgcttatctg gtaaatcata
      121 aaagagttca aggcttgata aaagtactca atttacaagc taaaatgcga cagaaacgaa
      181 aataaaggag acgttggtaa gaaggcagag aatctcattc aaggccaatt tgaaggctct
      241 aaaacaatgg aaaagtgcta cacagatgtg acagaatttg ccattccagc aagtactcaa
      301 aagctttact tatcaccagt tttagatggc tttaacagcg aaattatcgc ctataatctt
      361 tcaacttcac ccaacttaga acaagtacaa acaatgttgg aacaggcatt cacagagaag
      421 cactacgaga atacgattct ccatagtgac caaggctggc aataccgaca cgattcttat
      481 catcggttcc taagagtaag ggaattcaag catctatgtc acgcaaggga aacagccaag
      541 acaacggtat gatggagtcc ttctttggga ttctgaaatc ggaaatgttt tacggttatg
      601 agaagtcgtt tcagtcgctt aagcaattgg aacaagctat tgtagactat attgattatt
      661 acaacaacaa gagaattaag gtaaaactaa aaggacttag tcctgtgcag tacagaacta
      721 aatccttcgg ataaattatt tgtctaactt tttggggtca gtacacctaa aactttgatg
      781 atatacgttt tcttgtgaga atatttactt catttttgcc taaaattcaa tgtttactca
      841 gtatttggat tatgaaaaat cgaggtctaa atctagatac attttttctg aagacaaatc
      901 attttgacca ccgagcaaga gattttcaaa aaaagctgtt aaaaactcag aacgtcgctg
      961 taaaatcttt gcattatcta ataccaaggc atcacgaaaa tatttggaat gttgctgaaa
     1021 tggtgtatta tcaatatcaa aaccaaactc acgaagatac tgaatcaaaa agaccgttac
     1081 tgtccgagtg tttccttcgc gaaatggatg aatctgccag attcctgaaa taaaatgctg
     1141 gatttgttta accacatccg cctgagttag tgtcgcatat gcaacttgtt tttcctgatt
     1201 aaaatcataa tctaaggtca tttgaatcat ggagtaatca gagtacacaa cactttcacc
     1261 attcaaaaca ggttcattct ttgtgatatt ggtctgacga aattgcccca ccggaataga
     1321 gggttcaaat atatcttgaa acaactcctt atgaatagca agtaaggtcg caggactaaa
     1381 gctaaagcct cttcgagaca atagttctac aatacgtaga gaaaccaagt ctgcctcttc
     1441 cgtacttgca tcaatagtat ggtgataagc cgttgcatcc tcataaacct gctcataagt
     1501 cagttctccc cgggactgtt tctcagccaa agattccata tacgctgatg gcactagatt
     1561 gtcaactttc tgcagaccaa aacctatccg ccataaatca cgcttcgctt cataagacaa
     1621 gtttggattg tcaatgttgt aagttgattg catagaaata tcctttcaat tgtttttctt
     1681 cattaccagt aaatcacgaa attggttgga aactggctta aactataaaa agtcctcaca
     1741 at
//
LOCUS       DAVKAO010000126         1728 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKAO010000126 DAVKAO010000000
VERSION     DAVKAO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.126"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..1043
                     /locus_tag="VWX16_001990"
     CDS             <1..1043
                     /locus_tag="VWX16_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9159276.1"
                     /translation="YGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPIYKGELEKGYQFDGWKISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT          681 a          214 c          323 g          510 t
ORIGIN      
        1 tctacggaga tttacacatt gataacacta gagattttaa tattaagctg aatgttaaag
       61 acggtgacat catggactgg ggaatgaaag actataaagc aaacggattt ccagataagg
      121 taacagatat ggatggaaat gtttatcttc aaactggcta tagtgatttg aatgctaaag
      181 cagttggagt ccactatcag tttttatatg ataatgttaa acccgaagta aacattgatc
      241 ctaagggaaa tactagtatc gaatatgctg atggaaaatc tgtagtcttt aacatcaatg
      301 ataaaagaaa taatggattc gatggtgaga ttcaagaaca acatatttat gtaaatggaa
      361 aagaatatac atcatttgat gatattaaac aaataacaga caagacacta aacattaaga
      421 ttgttgtaaa agattttgca agaaatacaa ccgtaaaaga attcatttta aataaagata
      481 cgggagaggt aagtgaatta aaacctcata gggtaactgt gaccattcaa aatggaaaag
      541 aaatgagttc aacgatagtg tcggaagaag attttatttt acctatttat aagggtgaat
      601 tagaaaaagg ataccaattt gatggttgga aaatttctgg tttcgaaggt aaaaaagacg
      661 ctggctatgt tattaatcta tcaaaagata cctttataaa acctgtattc aagaaaatag
      721 aggagaaaaa ggaggaagaa aataaaccta cttttgatgt atcgaaaaag aaagataacc
      781 cacaagtaaa ccatagtcaa ttaaatgaaa gtcacagaaa agaggattta caaagagaag
      841 atcattcaca aaaatctgat tcaactaagg atgttacagc tacagttctt gataaaaaca
      901 atatcagtag taaatcaact actaacaatc ctaataagtt gccaaaaact ggaacagcaa
      961 gcggagccca gacactatta gctgccggaa taatgtttat agtaggaatt tttcttggat
     1021 tgaagaaaaa aaatcaagat taagacaaaa gctatagaaa aaaatggttt atgtactgag
     1081 attagatagt gaggtgatga catagttttg tgaaaacagc catttataac tcaattattt
     1141 agcttacttc actattgata ctatttggag ttattaatgg acttagttta gataactaat
     1201 gaattgattg aaagggttag tattgacaat attggtcata ttgattagaa aatagagtct
     1261 atcaaaattt aaaggctaat agaggtgatg agacaatttc aaggaattaa gtagcgtttg
     1321 gttgcttaat tcctttttat ttggaaggaa gtttttcgta accgcccaat aacgaagtct
     1381 attgggcggt tacgatatga tcactgactt cgtcagttct atctacaacc taaaaacagt
     1441 gttttgaaca acctgcggct agcttcctag tttgctcttt gattttcatt gaatattaga
     1501 acagcaaaaa tgcttggagt atttgtttgt gtgtttgttt ttatataaca aactataaac
     1561 aaaacaaaaa tataaaaaaa gagacaaaaa agaacaaaaa gtaattgaca acggtttcat
     1621 atagtgttat acttatggca taaagttaat ggaaagaagg gaaaacctta tggtattaga
     1681 ttatttcttt gacaaaaacc ttgtgttttg cttagaagcg gataatca
//
LOCUS       DAVKAO010000127         1682 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKAO010000127 DAVKAO010000000
VERSION     DAVKAO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.127"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            74..529
                     /locus_tag="VWX16_001991"
     CDS             74..529
                     /locus_tag="VWX16_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9159277.1"
                     /translation="MQWQTQLSEKKANTDYIARYGVYAVIPNPEQKQIVLVQEPNGAW
                     FLPCGEIEAGENHQEALKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLY
                     EATPFKEVQKPLENFNHIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            694..993
                     /locus_tag="VWX16_001992"
     CDS             694..993
                     /locus_tag="VWX16_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9159278.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1254..1577
                     /gene="tnpB"
                     /locus_tag="VWX16_001993"
                     /pseudo
     CDS             1254..1577
                     /gene="tnpB"
                     /locus_tag="VWX16_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          574 a          330 c          293 g          485 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcatttca ctataataat aacgatacta
       61 gataatgagg aaaatgcaat ggcaaaccca actttcggag aaaaaagcga atacagacta
      121 tattgctcgc tatggcgtat atgcagttat ccctaatcct gaacaaaaac aaattgttct
      181 tgttcaagaa ccaaatggtg cttggttcct accatgtgga gaaattgaag caggtgaaaa
      241 tcatcaggaa gccctaaagc gtgagttgat tgaagagctt ggtttcacag cagaaattgg
      301 tacctattac ggacaagctg acgaatattt ctattctcgt catcgtgata cctactacta
      361 caatcctgcc tacctctatg aagcaactcc tttcaaagaa gtacaaaagc cactagaaaa
      421 ctttaatcat attgcctggt tccctattga cgaggctatc aaaaacctta aacgtggtag
      481 ccataaatgg gccattgaat cttggaaaaa acagcataag attgactaat actcaatgaa
      541 aataaaattc aaaccacgtc agcgtcgcct taccgtactc aagtatagcc tgcggctagc
      601 ttcctagttt gctcttttat tttcattgag tataaatcaa gcgactttat tcaaaaagtg
      661 ctataataga attagaaaat cggaggaaat attatgaagc ttatcaatac gacaaactca
      721 cactcgcaac ttgtaaaaag tcagctagaa agtaccgatg caacccttgt tgaggtttat
      781 tctgcaggga atacagatgt tatttttacc caagctccgc ttcactatga aatcctcatc
      841 tcaaacaaac accgtgctat tcgtgagcct gaaatcgaaa ccattcaaga atttttcttg
      901 aaacgtaaaa ttgataagac ctctgttgat gaagccaata tcaagacact ctactcagag
      961 aaattaattg gaatttcaat cccaatcaag taagaaaatt gagtaaaaac tcgatttgag
     1021 agaaagtcca ataagtcttt ccataataaa acgcatagta gcaagagttt ctacacccac
     1081 tattatgcgt ttttatcatt ttgaaatcta ctgctaatca atgaagaagc aaagatcaaa
     1141 ctaggcagct agttacaagt tacgtaagcg cgtcataaca aggtatctat cctaattccc
     1201 caacttccag tatactagat aaaaaataat tatcggagga aaggtattgt actatgataa
     1261 ttcaactaag tgatttaggt caagttcacc ttgtttgtgg caagacagat atgaggcagg
     1321 gaatagactc attagcctat gtagttaaaa cccactttga attggatcct ttctccggtc
     1381 aagcctttct cttttgtggt ggacgtaaag accgctttaa agccctttac tgggatggtc
     1441 aaggattttg gctactatat aaacgctttg agaacggaaa actgacttga ccaagtacag
     1501 aaaaggatgt caaagctctc acaccagaac aagtagactg gcttatgaag ggcttttcta
     1561 tcactcccca aatatagtgg atttaaacta gaatagtaca cccctacttc taaaacattg
     1621 ttagaaatcg atttgactgt cctgatcaat ttgtcctgtt cttgtttcat tttactatag
     1681 aa
//
LOCUS       DAVKAO010000128         1617 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKAO010000128 DAVKAO010000000
VERSION     DAVKAO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.128"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..211)
                     /locus_tag="VWX16_001994"
     CDS             complement(<1..211)
                     /locus_tag="VWX16_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159279.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATI"
     gene            complement(242..556)
                     /locus_tag="VWX16_001995"
     CDS             complement(242..556)
                     /locus_tag="VWX16_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU9159280.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            complement(566..1591)
                     /locus_tag="VWX16_001996"
     CDS             complement(566..1591)
                     /locus_tag="VWX16_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9159281.1"
                     /translation="MYNFSSDFQLIEGLITHLMTLQVRLESRITLNNPLVDEIKQNYS
                     DIFFMTREILANMDKFLEWTISDDEVAYVSLHFLAAMERSKESTKFNILAICATGFGA
                     AQMLRNRLETEFGKRVEVVDVIGYYELNQEKLKGIDFIVSAVDLSNLYFQIPVFKVSV
                     FLKSDEMEMIRKAMVQMQVSSYVQSSKINKFENNGFRQYFSKENFLICTESDKVNLLE
                     KMVESLSVGESNEFEQSLLYGIKQREELSSVVFSEKIAVPHPIQPFGTEEKVSVAICK
                     DSLLWDNQSSHVQLVFLLSPSIYGNEGLATVTKKIVSLTENDELQNQLISCNNFEDFI
                     NIFEKIK"
BASE COUNT          530 a          305 c          214 g          568 t
ORIGIN      
        1 caatagttgc taaaatagaa aatactatga gcataatggg gaaaataata ataggcgagg
       61 gattaataaa ctgattcaaa agccaataaa tattcccaaa aaagaagagt gctattgaat
      121 aacgtagaag aagatatcga ttaaaaaaag tattagttag agccatctct cgacgttgtt
      181 gttcaatctt ttgtcgttct tttttatcca tatcatttcc tccttatata acaacacata
      241 tttagttaac tttcttataa agagctaaca tttcctttgc tacttctaat aatgtcatag
      301 tggtcattaa atgatcttga gcatgtacca tgataatttc aattttaatt tccactccac
      361 ttgcgtattc ttgcaagagt ttggtttgtg catgatgcgc ttcaagaatt atctcatttg
      421 attgatttaa tttactttct gcatcatcaa aactaccttc tctcattttt gcaaatgctt
      481 catgtatttc tgaccttgca tttcccgaat gcaggataat ttcaaatgct gcaacctgca
      541 gttcctcttg attcatatga acctcctatt ttatcttctc aaatatgtta ataaaatctt
      601 caaagttatt gcaagatatt agctgatttt gcaattcatc attctctgtc agagagacta
      661 tctttttagt cacagttgcc aaaccttcgt tcccatatat tgatggagat agaagaaata
      721 ctagctggac atgtgaactt tgattatccc agagtaacga atctttacaa attgcaaccg
      781 aaaccttttc ctctgtacca aagggctgaa taggatgcgg aactgcaatt ttttcagaaa
      841 aaacaactga acttaattct tcgcgctgtt taattccata aagtaaagat tgttcaaact
      901 catttgattc accaacagat aaactctcaa ccatcttttc aagtaaattt accttgtctg
      961 attcagtaca tattaaaaag ttttctttac taaaatactg tctaaagccg ttgttttcaa
     1021 atttgttaat ctttgatgat tgtacataac tagaaacttg catctgaacc atagcttttc
     1081 taatcatttc catctcatca ctcttaagaa acacactaac tttaaaaact gggatttgaa
     1141 aatatagatt tgataaatca acagctgaca ctataaaatc tattccttta agtttttctt
     1201 gattcaattc ataatagcct attacatcaa caacttctac tcgcttccca aactccgttt
     1261 ccaaacgatt tcttaacatt tgggctgcac caaatcctgt tgcacaaata gcaagaatat
     1321 taaacttagt actctctttg ctacgttcca tagcagctaa aaagtgaaga cttacatatg
     1381 ctacttcatc atctgatatt gtccactcca agaacttgtc catatttgca agaatttctc
     1441 tagtcataaa gaatatatca ctataattct gtttaatttc atctaccaaa gggttattta
     1501 aggtaatccg gctttctaaa cgtacttgta gtgtcattag atgagttatc aatccttcaa
     1561 ttagttggaa atctgaagaa aagttataca tatcatctaa tcctaaattc tgaaatg
//
LOCUS       DAVKAO010000129         1576 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKAO010000129 DAVKAO010000000
VERSION     DAVKAO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.129"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            27..1373
                     /gene="gdhA"
                     /locus_tag="VWX16_001997"
     CDS             27..1373
                     /gene="gdhA"
                     /locus_tag="VWX16_001997"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9159282.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWNYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGILYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDDRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          477 a          305 c          329 g          465 t
ORIGIN      
        1 aaatatgaca ataaaaggag aacgatatga catctgctaa agaatatatc caaagcgtgt
       61 ttgaaactgt gaaagctcgt aacgggcacg aggctgaatt cctccaagct gttgaagaat
      121 ttttcaacac tttagaacct gtatttgaaa aacaccctga gtatatcgaa gaaaatatct
      181 tggcacgtat tactgagcct gagcgcgtga tttctttccg tgttccttgg gttgaccgtg
      241 atggaaaagt tcaagtaaac cgtggttacc gtgttcaatt caactcagct gttggaccat
      301 acaaaggtgg acttcgtttc cacccaactg taaaccaagg gatcttgaaa ttcctcggat
      361 ttgaacaaat ctttaaaaat gtcttgactg gacttcctat cggtggaggt aaaggtggat
      421 cagacttcga tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc caaagcttca
      481 tgactgaatt gcaaaaacac atcggaccat cacttgacgt acctgctggt gatatcggtg
      541 ttggtggacg tgagattggt tacctttacg gtcaatacaa acgtcttaac caatttgatg
      601 ctggtgtctt gactggtaaa cctcttggat ttggtggtag cttgattcgt ccagaagcaa
      661 ctggttacgg tttggtttac tatactgaag aaatgctcaa agctaacggt aacagctttg
      721 ctggtaagaa agtcgttatt tcaggttctg gtaacgttgc tcaatatgct cttcaaaaag
      781 caactgaact cggtgcaact gttatttctg tttctgactc aaatggttat gtcatcgatg
      841 aaaatggtat cgacttcgat cttttggttg atgttaaaga aaaacgtcgt gctcgtttga
      901 ctgagtatgc agctgaaaaa gcaactgcaa cctaccacga aggtactgta tggaattacg
      961 ctggaaacta tgacattgct cttccatgtg ccactcaaaa cgaaatcaac ggtgaagcag
     1021 ctaaacgttt ggttgctcaa ggcgttatct gtgtatctga aggtgccaac atgccgagcg
     1081 accttgatgc catcaaagtt tacaaagaaa atggtatctt atacggacct gcaaaagctg
     1141 ccaacgctgg tggtgtagcc gtttcagctc ttgaaatgag ccaaaacagt cttcgcctct
     1201 catggactcg tgaagaagtt gatgaccgtc tcaaagacat catgacaaac atcttcaaca
     1261 cagctaaaac gacttcagaa acatacggtc ttgataaaga ctaccttgca ggagctaaca
     1321 ttgctgcctt tgaaaatgta gcaaacgcta tgattgccca aggtattgtt taagatttat
     1381 ttgcttaatc ctcaatcact acgattgggg atttttagtt gagttagaca gttgggaaat
     1441 tctaattaaa tgaaataaaa actgaacaaa ttgattgcgg aattcaaacc aatttctaac
     1501 atatttctag aaattaattt gacttttcta atcgatttgt tcatatctta tttcaattta
     1561 ctatacttac ttaacc
//
LOCUS       DAVKAO010000130         1403 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKAO010000130 DAVKAO010000000
VERSION     DAVKAO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.130"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..585)
                     /locus_tag="VWX16_001998"
     CDS             complement(<1..585)
                     /locus_tag="VWX16_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9159283.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFN"
     gene            complement(885..1067)
                     /locus_tag="VWX16_001999"
     CDS             complement(885..1067)
                     /locus_tag="VWX16_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9159284.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIASELVAGLR"
     gene            1126..1403
                     /locus_tag="VWX16_002000"
                     /pseudo
     CDS             1126..1403
                     /locus_tag="VWX16_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          479 a          236 c          188 g          500 t
ORIGIN      
        1 gttaaactca gtacaataga aatcttgaat gattttctca attcccataa ttatttcagt
       61 gattttatac gactcttgaa aattattttc taaattagca ttaacaaaat gtagcgctat
      121 gaatgcagct tcgtcaattg gaagttcaat acctaactct ttttttatta tttgtaaagc
      181 ccttttccca agctcaaatt cttctttata atatttcgag atttcccagc gtaggggatt
      241 cgaaataata actccttcct tataacgttc tatactcgaa gaaatatgat ccgttagatt
      301 tatatagata atctcatcaa aattattacc taatgttatt ttacctagat taattatttt
      361 ttcggtgatg tacaccaact ccaaaggaac gttaataaac atctctgtaa aacgggtcat
      421 attatctcta tttttgagaa aaaaactttt ctcaatatca attggattta ctttatctcc
      481 aatttttctt ccaaaagcta ttccccttcc aaaaagaaga atatcgatat cttttttact
      541 ttgcgcaatt acggcattgt ggtttagtat tcgttttatg atcataaaat ttccctttta
      601 aaataaaagg aacctactta aaaacatcaa gaaacttctt ctgatgtgat taaataggtt
      661 cctgctcact tgtattagtg ataacatcct tttatttcaa tttttaatgg tgacatgaag
      721 cttaaatcat taataactct gtaaccattt tcattttaag atatttcaat ctatttgtca
      781 agtacttata gaaatttttt tattttgtgt gattcgaatg tgataatggc aacagtctca
      841 ctaaagaaat gaaaatcata catagaaatt tttaaaaatt ttctctatcg taaacctgcc
      901 actagttcag aagctatact agctgattcc aaacgtaata tggtcatctc atctaccttc
      961 cctaataatg gcattgcacc ataccaaaca atagtcttat tcaccaaaag aataggcgtt
     1021 actatttttg attgagtaaa agacacttta attttctggc tcttcaactg tgaactgcac
     1081 cccaaaagtt agacagaaaa aatctaactt ttggggtgtt ttagtatgaa actaacttat
     1141 gatgataaag ttcagatcta tgaacttaga aaacaaggat atagcttaga gaagctttca
     1201 aataaatttg ggataaacaa ttctaatctt aggtacatga ttaaattgat tgatcgttac
     1261 ggaatagagt tcgtcaaaaa aggaaaaaat cgttactatt ctcctgattt aaaacaagaa
     1321 atgattcata aagtctgaca tgaaggctgg actaaagata gagtttctct tgaatactgc
     1381 ctcccaagtc gtacgatact tct
//
LOCUS       DAVKAO010000131         1348 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKAO010000131 DAVKAO010000000
VERSION     DAVKAO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.131"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            57..1235
                     /locus_tag="VWX16_002001"
     CDS             57..1235
                     /locus_tag="VWX16_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159285.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT          461 a          264 c          285 g          338 t
ORIGIN      
        1 cggctagctt tctagtttgc tctttgattt ttattgagta ttagaaagga gacctcatga
       61 cacttccagt tagaaaatcc ctgcacgatg cagttttaca ggcttcgaaa gcggatactt
      121 gggaacaagc taccaaggaa tggaatgaag tttccttgat ttttaacggt atcggccgta
      181 gcaattgtgt ctgtggaaat gccatcaaat acgcttatga actctttaac ggtgtcacag
      241 gccaacgcct ctttccaatt ggtagcgact gtgttcgtca ttttcatcga ttgtcccttg
      301 accagcaatt ggaagaggaa gaaaaactgc tcagaaaggt tgaaaatcta accagaaaag
      361 ctcagaaaaa ggaaaaaatc aaggtcaata aaagtgactt tgacgagcga cttctaaaat
      421 ggctctggga aaaaggtgtt ttcaaaccca atcgtggcaa tcaatttgcg cctgagagag
      481 actaccagct tttcctagaa gtctttcagg gaggaagttg gaccaaggcg gaaccaaaga
      541 aaaaggctcg gatggaagaa gtccttgaaa agtgtatcaa acccttttta cttgggaaat
      601 cggatgacca actctacctt gtcaagctag gcaaggagaa aattgactac gaacaagaac
      661 tccgtatcca ggcagagaaa gaacgcaaga agagggataa aatcgccaag caatatgcag
      721 acaatctggt tctagccatg ggacccgcag agcgtgctta tcaagattac tttggcttta
      781 cagagaccct gactcaagaa gaacgcaagt gggaaaaaat tctttttggt aaaaatagag
      841 cagaacgggc tatcaaggcc aaacaatacc aaaaagagct ggaaaaagac caacgaattg
      901 ccagtcagga tccaattgaa agaaaacaga agcagacttg gctcctcaat tcctattttc
      961 gtgagcttcc tgaagaaaaa gctcgatttt ctaggctctt attagaatat cgaaaaagtg
     1021 gtgaagtacc cttttcaact gaatacctgt cagatcatct catcgacttt ttctataaaa
     1081 tgaaagcctt tgaatttgaa atcgcaccag aacaagtccg agattttcta aaaaaaagcc
     1141 tgcaggaaga ccatcgctca tcagcacaag gaagctggat agaaggaatt ctcctcaact
     1201 gcctcaaacc atttttagaa cgattagtga tatgagtaac acctaccgtg taaatttcat
     1261 ggtaggtgtt attgtacccc aaaataatat aaaacaaagt atgaaagcgc atctcaattt
     1321 taagactttt ttgtaaatta agagtata
//
LOCUS       DAVKAO010000132         1315 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKAO010000132 DAVKAO010000000
VERSION     DAVKAO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.132"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..434
                     /locus_tag="VWX16_002002"
     CDS             <1..434
                     /locus_tag="VWX16_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9159286.1"
                     /translation="ILNDVVSRYISQGNKSIIISMGYLGSLLSKKYMEDSDIREFADF
                     ILVSKIKPIDWEYWENFLRKYETVYIIEENTLTGGLGEQFKNYFFGLGINIISFGIKP
                     HFGETGDYETLLTNEGLSPDKIFEKIRRINHVQSSEKSIWR"
     gene            400..>1315
                     /locus_tag="VWX16_002003"
     CDS             400..>1315
                     /locus_tag="VWX16_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU9159287.1"
                     /translation="MYNLVKRVFGDDNFVKSDGIYLYTDDNKEIIDSCSNSMNVNLGY
                     GVLEIEKVIHEQIRKINFMHTGKGTTYESLMLANRLHDIIKPYGNYKVYFATSGSDCI
                     EAALRISVLYQQKNKRNQESNTRFATFEGSYHGSTLGALSVSGHRKFQKLYNGYIGNC
                     LTIPTRWNLEWEMDFTDITAFVLDSMITNPIGSEMIDRDYLNYLVQICKESGVITIID
                     EIATSIGRLGCFFGFEDSGISPDIVCISKGLGVGYANIGAVIVKSNIIDSINSADILG
                     HTYNASPIDCKIAMTVLDYIEEHGIFEHV"
BASE COUNT          460 a          159 c          259 g          437 t
ORIGIN      
        1 aaattttaaa tgatgtagtc agtcgctata tcagtcaagg aaataaaagc ataattatct
       61 caatgggcta tctcggatcg ctactctcaa aaaaatacat ggaagattca gatattaggg
      121 agtttgctga ttttatttta gtaagtaaga tcaaacctat tgattgggaa tattgggaaa
      181 attttttacg caaatatgaa accgtataca taattgaaga gaataccctt acaggtgggt
      241 taggagaaca atttaaaaat tatttttttg gattaggaat aaacatcatt tctttcggta
      301 ttaaacctca ctttggagaa actggtgatt atgaaacctt acttacaaac gaagggcttt
      361 cccctgataa aatttttgag aaaattagaa ggataaatca tgtacaatct agtgaaaaga
      421 gtatttggag atgataattt cgttaagtca gatgggattt atctatacac tgatgataat
      481 aaggagataa tagatagttg ttctaattca atgaatgtca atctgggtta tggtgtgtta
      541 gagattgaaa aggtgattca tgaacaaata cggaaaatta attttatgca tacaggcaaa
      601 ggaacaacct atgaatcatt gatgttagct aatagattgc atgacataat aaaaccttac
      661 ggtaactata aagtgtattt tgcaacttct ggttcggatt gtattgaagc tgctctcaga
      721 atatcagtgt tatatcaaca aaaaaataaa agaaatcaag aatccaatac tagatttgcc
      781 acatttgaag gaagttatca cggttctaca cttggtgcgt taagtgtatc tggtcataga
      841 aaatttcaaa aattatataa tggctatatt ggtaattgtt taactattcc tacgcgatgg
      901 aacttggaat gggaaatgga ttttacagat attactgcgt ttgtattgga tagtatgatt
      961 actaatccaa taggctctga gatgattgat agagactatc taaattattt ggtgcaaata
     1021 tgtaaagagt ctggcgttat aacaattatt gatgaaattg ctacatcaat tggacgactt
     1081 ggttgctttt ttggattcga ggatagtgga atttcgccag atatagtttg tatatctaaa
     1141 ggtttaggag ttgggtatgc gaatataggt gcagtaattg tcaaaagtaa cattattgat
     1201 tctattaatt ctgcagatat tcttggtcat acatataatg catcgccaat agattgtaaa
     1261 attgccatga ctgttttaga ttacatagag gaacatggta tatttgaaca tgtga
//
LOCUS       DAVKAO010000133         1180 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKAO010000133 DAVKAO010000000
VERSION     DAVKAO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.133"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            285..665
                     /locus_tag="VWX16_002004"
     CDS             285..665
                     /locus_tag="VWX16_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEU9159288.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVGL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGADWADGSLDGYIFA"
     gene            complement(1067..>1180)
                     /locus_tag="VWX16_002005"
     CDS             complement(1067..>1180)
                     /locus_tag="VWX16_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9159289.1"
                     /translation="NAKLIQKGFSLLELTEETSMLSHLKKEVETHYQPKKL"
BASE COUNT          364 a          144 c          248 g          424 t
ORIGIN      
        1 gctgttgctt tattattatc tcggaaaaac tttgattcgt taaatgttgt tcttataatg
       61 atgatcgtat cacaattgtt gttattagta tatttgataa aatcagggaa tcagtgatta
      121 gctgttattt gaattaagta aactgttaag cttcaagcag taactggttt taaatattta
      181 aaataagata aaggaaggaa gtctaattta ggtaactaaa caaagcctgc gatcacttgt
      241 taggaaacca tcctaaccat gcttaataaa aaaggagaaa ataaatggaa ctcgtattac
      301 caaataatta tgttgctctt gagcaagaag agatgatgta tcttgatggg ggtggtgttg
      361 gtcgtaactg gtggaatagt agaggtagtt ttgcaacagt tctggatgta ggtttggcca
      421 tctatagtgg tggtgcaaca atttattctg cttatgcgat aaaaaaagct atctcagcta
      481 atagaggggc tattacgaga acattacgta gtttaataat taaacatgta ggtagtgcag
      541 ctggccattt agtcaatact gcactaaacg ttgcactaac tgttactgga ttttcactag
      601 gtggagcaat cgcatatggg gctgattggg ctgacggtag cttagatggt tatatttttg
      661 cttaaaatga aacatttttt tgcaggaatt ggtgaaataa actttgtaag ttatttattg
      721 tatatatgtg taggaatagc cccttttttt cacgtatata taataggttc tgaaatgaat
      781 tttgtgaaaa tagtgttatc tattttggga attatatttg tttgtatttt aactattgct
      841 cgaatttata ggactttttt ctataaagaa tagtttggac ttgaaatgta tttaaaaaca
      901 agaggttggt agattatcag cctctttctt gtcgttgaaa aagtgagata tattgacttt
      961 tgaaaaaaat gtcataattg agttgggata aacgtaaaag tcatactttc tcgttaaatc
     1021 gttcggcgat tcttatgaaa aggtcgagga gctccctcga ctttttttat aatttctttg
     1081 gttgataatg ggtctctact tcttttttga gatgagacag catagaagtt tcctcggtca
     1141 gctctaggag tgagaaacct ttttggataa gtttagcatt
//
LOCUS       DAVKAO010000134         1154 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKAO010000134 DAVKAO010000000
VERSION     DAVKAO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.134"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            <1..>1154
                     /gene="phtD"
                     /locus_tag="VWX16_002006"
     CDS             <1..>1154
                     /gene="phtD"
                     /locus_tag="VWX16_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9159290.1"
                     /translation="AQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRS
                     NHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEE
                     NGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLAR
                     IHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQ
                     ITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAE
                     RAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVE
                     VKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPN"
BASE COUNT          372 a          248 c          244 g          290 t
ORIGIN      
        1 gcgcaaatca caagtcgaac cgccagaggt gtagctgtcc ctcatggtaa ccattaccac
       61 tttatccctt atgaacaaat gtctgaattg gaaaaacgaa ttgctcgtat tattcccctt
      121 cgttatcgtt caaaccattg ggtaccagat tcaagaccag aacaaccaag tccacaatcg
      181 actccggaac ctagtccaag tctgcaacct gcaccaaatc ctcaaccagc tccaagcaat
      241 ccaattgatg agaaattggt caaagaagct gttcgaaaag taggcgatgg ttatgtcttt
      301 gaggagaatg gagtttctcg ttatatccca gccaaggatc tttcagcaga aacagcagca
      361 ggcattgata gcaaactggc caagcaggaa agtttatctc ataagctagg agctaagaaa
      421 actgacctcc catctagtga tcgagaattt tacaataagg cttatgactt actagcaaga
      481 attcaccaag atttacttga taataaaggt cgacaagttg attttgaggc tttggataac
      541 ctgttggaac gactcaagga tgtctcaagt gataaagtca agttagtgga tgatattctt
      601 gccttcttag ctccgattcg tcatccagaa cgtttaggaa aaccaaatgc gcaaattacc
      661 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
      721 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      781 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      841 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatca ggattcagga
      901 aatactgagg caaaaggagc agaagctatc tacaaccgcg tgaaagcagc taagaaggtg
      961 ccacttgatc gtatgcctta caatcttcaa tatactgtag aagtcaaaaa cggtagttta
     1021 atcatacctc attatgacca ttaccataac atcaaatttg agtggtttga cgaaggcctt
     1081 tatgaggcac ctaaggggta tactcttgag gatcttttgg cgactgtcaa gtactatgtc
     1141 gaacatccaa acga
//
LOCUS       DAVKAO010000135         1140 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKAO010000135 DAVKAO010000000
VERSION     DAVKAO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.135"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..210)
                     /locus_tag="VWX16_002007"
     CDS             complement(<1..210)
                     /locus_tag="VWX16_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159291.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLMS"
     gene            complement(207..560)
                     /locus_tag="VWX16_002008"
     CDS             complement(207..560)
                     /locus_tag="VWX16_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9159292.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSPGT
                     FIALTGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            complement(616..>1140)
                     /locus_tag="VWX16_002009"
     CDS             complement(616..>1140)
                     /locus_tag="VWX16_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159293.1"
                     /translation="IPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQNKWQKEDSFES
                     DLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCT
                     GLVELLLYEMSISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNFDFCMNREYHK
                     MKSRKLLLSNKKIV"
BASE COUNT          383 a          224 c          151 g          382 t
ORIGIN      
        1 actcattaac agaatcgaat ttataactag acagatagat actattttat ttttcacaga
       61 atatagaggt agtgatacta ttgagtagat aaaagctaaa aaaataagca aaaaatactc
      121 cgtgtcattt ataatcgccc tgaatgataa aaatatatac acccaacaca cgataatgtg
      181 tatttttcga atcttatcta cattactcat cttactgtcg cagatatgaa tccatcccaa
      241 ggataagggt tcccgctaat atcacaacct ctattaagag caccatatcc aatacagtaa
      301 gctattcttc ctgccgcccc agccaataca cctgctgctg caccaataag ccaacctcca
      361 aggcctccaa ccttaatata gtttattagt tttttggtta aaactgcagc tccagtcaat
      421 gctataaaag ttcctggact catagctaaa gctacgcaaa ttccttgaca agcacgcttg
      481 cttaaataag ctcccccatc aagatacatc atttcttctt catcaatcac aacataatta
      541 tttggtaata caagttccat ttgttttctc ctccttagtt gaaattgttt catttgtttt
      601 acaaatagga cgaacttaaa ctatcttttt attactcagt aataatttcc gagatttcat
      661 tttatgatac tctctattca tacagaaatc aaaattttgc cttaaccaac ttgtataata
      721 gaaaaaatct aattgattga ttctgatgag ctgtattcta attttatcaa ccttttcttg
      781 ccagttgtta ttgttactaa tagacatttc atacagcagt aactcgacaa gacctgtaca
      841 cagaagggtg ttatgagaat cttcgatttt tttcaaaata tcctcaaaaa gttcactatc
      901 cgttaaaaag gtggattggt tcatcgcatc atacagctga agagacatta agtcagaata
      961 acttagctct gatttttgta tcttatcctt taacgttttc gtcaaatcac tttcaaacga
     1021 atcttctttt tgccatttgt tttgattaaa aagaaattcc gtcttattaa aaacgagatg
     1081 attgatatcc attatagtcc agaaaaatat tgtagtaagc atgatgattg caaaaggaat
//
LOCUS       DAVKAO010000136          959 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKAO010000136 DAVKAO010000000
VERSION     DAVKAO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.136"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            1..833
                     /locus_tag="VWX16_002010"
                     /pseudo
     CDS             1..833
                     /locus_tag="VWX16_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863411.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase"
BASE COUNT          390 a          132 c          183 g          254 t
ORIGIN      
        1 ttgcttcaga aggattagag aatttacagg aacagtacca tgctttccaa gaagaacgag
       61 ataaggataa agtatccact gaagagattg aggaagcctt taagacattt aaggaaaaat
      121 gagataccgt tcgtgaattt gaagtggaac ttgcagagaa ccaaatagag aagctagttg
      181 ataagggcgt ctatatcaag gtgtctttcg gtgtcaaaca gagcggtctt attttcattc
      241 ccaactatca attggatatt atggaagaag aaaatcataa aaaatataaa gtctacattc
      301 gagaaacgtc ttcttacttt gtctataata aagaaaacat ggataataat tgtttcatca
      361 agggtaggac cttaatcaga caattgagca acgatagcca aaaactcccc tatagacgac
      421 caactttaaa aagtcttcaa gagaagattt ctgaaattaa tcttatgatt gaactatcta
      481 acacgaacaa acaataccaa gaaatcaaag atgagcttgt attagaaatt gcagagattg
      541 atatgaagct agaagaaacc caagaaaaaa tcgccacctt aaacaagatg gcggaagtcc
      601 ttatcaattt gaagagtgaa gatcatgaga ctagaaaatt agccaggtat gacttttcaa
      661 aactgaactt aacagaatca acttcattag aaaatgtgaa cgaagaaata agagtattgc
      721 aagaaaactt agactattat ctttatgagt ttgagaaaag agcaataaga ttagaaattt
      781 ttgtaagcac gttaaatatg gaaaaagatg ttttcgtaat tgacaaattt tagaggaggg
      841 ataaagctag aaaggctgga ttatgcttga tttaaggaac tgcaatacat ggagcacact
      901 catatggttt aagcacaaat aatttttgac aataggatta aaaaattgta aaataaaaa
//
LOCUS       DAVKAO010000137          944 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKAO010000137 DAVKAO010000000
VERSION     DAVKAO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.137"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..709)
                     /locus_tag="VWX16_002011"
     CDS             complement(<1..709)
                     /locus_tag="VWX16_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9159294.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFSLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVVLSNGQPTFLSTILSFVLPVIIDPVFEETLDRGYFMNTFFPKSK
                     YYLDVILSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFLT
                     FSIMQNLSGFLFTIISII"
     gene            complement(727..912)
                     /gene="briC"
                     /locus_tag="VWX16_002012"
     CDS             complement(727..912)
                     /gene="briC"
                     /locus_tag="VWX16_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9159295.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
BASE COUNT          359 a          169 c          148 g          268 t
ORIGIN      
        1 tgataataga tataattgta aacaaaaatc cagataggtt ttgcatgatt gagaaagtta
       61 aaaaactatg gcagagaatc gttaatctca gattgtcggt agaacgataa acaagggcaa
      121 aaaagaaacc aatcaaacta taatataata aactaattgg atctctgtga gatagtatca
      181 aatgactaag cccaaagata aggcctgata agatgacatc taaatagtac tttgacttag
      241 gaaagaaggt attcataaaa taccctctat caagagtctc ctcaaaaaca ggatcgatga
      301 ttacaggcag gacaaaagat aagatagtcg ataaaaaggt tggttgtcca tttgaaagaa
      361 ccacggtaaa atactcatca tgaatattcc tatgattaat caaatgagca tagcgtgccc
      421 aaaaattacc gagaatctga taaaccacat aagttgcaaa taaggagaag acaaatgacc
      481 agttccagct ctttttctca aagataaaga gcatcttttt cttttttaac ctccaaatta
      541 atagaaggaa acttcccact aatcccattg ttaaaataag agaatagaca tcagctccta
      601 accctaaaat gatcgtcaca tacaatccaa ttgtttgtgg taaataggta gatagtaaaa
      661 taataagcaa aaatattcca aattgtctta gtttctttgt gtttctcatc gtactttttt
      721 gaaagattac cctgttcgga agccgtactt ccaagcatct atataagaat taagtgcccc
      781 ttgcctcata taaggagcaa attctctata atataaccat ctactatatc catcttccca
      841 aacagcaaga ccacctgaag tttgctccaa gtcctcagtt gaaataactg taaatgtttc
      901 tgtacctgtc attgcaagta ccttcttaaa atatagtgat ttga
//
LOCUS       DAVKAO010000138          932 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKAO010000138 DAVKAO010000000
VERSION     DAVKAO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.138"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(85..834)
                     /locus_tag="VWX16_002013"
     CDS             complement(85..834)
                     /locus_tag="VWX16_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9159296.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFQYVSDSSMVGYMGKTKELKMYQEELDKKHYIDRISSISCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          319 a          183 c          106 g          324 t
ORIGIN      
        1 tttatattta gatgcaaata gtaacgcgta ggccagccca acatgcccca aaccaattac
       61 tgctatattc ataaaactac ttccttattt cttaatccaa aatctaatag aataagctgc
      121 cccattcctt aaatacaact ctttaatatt gtttaaaagt ttttcaactg atttccagat
      181 tatcaaaatc tgagatttat agcacgatat tgatgatatt ctatcaatat aatgtttttt
      241 atcaagttcc tcttgataca tttttaattc tttagttttt cccatataac caaccatact
      301 actatcactt acatattgga agtcctcata atatattact ttataacgca taaattcaag
      361 cgcccttcca atactattca caaaaacatg atcaacatgg tcaccaagtg aaagcggaca
      421 atatacgaca catttgtcgt ctaaatgcat taacagctct tttatgatat cattctttaa
      481 tgtgtcctca ttttttaatt cactatagat atgacggtat agaaaattgc catttctatc
      541 tttcctatag agacattcat agtacgataa gtgtctaaaa tcacattgta gacgttcaca
      601 agctaacctg tcttctttct tcctttcttc aatcggatat ttcccaaggt tacacaactt
      661 atgaaattgc ttagcagagg gctgtagctg ttggctcaaa gggtaaccag aaaatatagt
      721 aataacaagt acaatttctc cttctgaagt taattttgaa atataatcac cacaggaaaa
      781 aattgcgtca tctaaatgtg gagataaaaa gatatactta gtattgttac tcataaccat
      841 tccctctaca atttatctaa aaactcactt agtgtctgat taaattccac atcatcaaaa
      901 aaattcacct tattcttaat aatgaatatt tc
//
LOCUS       DAVKAO010000139          924 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKAO010000139 DAVKAO010000000
VERSION     DAVKAO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.139"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            81..878
                     /locus_tag="VWX16_002014"
     CDS             81..878
                     /locus_tag="VWX16_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9159297.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
BASE COUNT          244 a          155 c          202 g          323 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atggtttctt cagaatttat ttcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt tttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taag
//
LOCUS       DAVKAO010000140          920 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKAO010000140 DAVKAO010000000
VERSION     DAVKAO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.140"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..758)
                     /locus_tag="VWX16_002015"
     CDS             complement(<1..758)
                     /locus_tag="VWX16_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159298.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLIQNQSKVDLDIFYKVLPLLYKEKYT"
BASE COUNT          349 a          155 c          122 g          294 t
ORIGIN      
        1 atagtatatt tttctttata aagcaaaggt aaaactttat aaaaaatatc taagtcaacc
       61 ttactctgat tctgaatcaa gtaggagtca agcaaatttt caaaaatagc taacaatatg
      121 attggtgtaa ggtttttata ttcacatgtc aagtattccg agtatcgtgc agatgatatt
      181 tcttttatat aactttttga aagcaagatt ttttcaaatt catcatctat tattaatctt
      241 ctatttccaa tccaaacggt attaccaccc aaataagatt gtgcaaatag caatatgtaa
      301 gaaaatatga acgtaaatac tgaaagaggt agaattctat gcgtaaaaaa atagacgata
      361 atagtagcca ggagtaaaga tatctctcgt gacaatcgaa ttcttttcag catctttttt
      421 aacagtccgt aaggttgtcc ttggcaatat tcttgaacta aattaatcgg aatgacatct
      481 ctcaccattt ctggaaagta gagtaagttt ataggttgaa atctccagct cccatcataa
      541 gtaaatggat atagaaccaa atacttaaaa gatttttttc ttataaactt aattatcaca
      601 atatgaatga ctgttgcaat gaagtatatt aaatataaaa gtaaaaccgc catcaatata
      661 cttacaaaaa tccccttgga ctcaatcaat ggaaaataaa atattgcaac tagaagcgaa
      721 taaagtcccg ctaataaata tttctgatca tctttcattc aaaacaccat attatttact
      781 aagaatacat caatacaata gagcaactct gtaagtcaat atttgataaa gcaaagcttt
      841 aaccccttcc ccagacgtta gcttttcttc aacccactac agttgacaag gagcccaaat
      901 tattgataaa aactttagtc
//
LOCUS       DAVKAO010000141          903 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKAO010000141 DAVKAO010000000
VERSION     DAVKAO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.141"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            1..805
                     /locus_tag="VWX16_002016"
                     /pseudo
     CDS             1..805
                     /locus_tag="VWX16_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            798..>903
                     /locus_tag="VWX16_002017"
     CDS             798..>903
                     /locus_tag="VWX16_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9159299.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSY"
BASE COUNT          328 a           82 c          191 g          302 t
ORIGIN      
        1 taagacagtg ataacatgcc atggatgggt tgaatataac ttaaaaaaga aattcttgac
       61 ttattttgat ttttggacat attttatggg agatgctttt atttgtgtat ctgaaactat
      121 gaaaaaaaag attggagagt ataataaaaa ataagaagat agttgcaata aataacggaa
      181 ttaatgtatc aaatagcgat ttagatgtag taggtgttca agactttaaa aaagaatttt
      241 gtattccaaa taacaaaaaa tcatttgtta tgttggaagg ttggatccag aaaaaaggcc
      301 ggatagattc cttgaatttg cagaaaaatt atttttagtc agagaagatg tgatttttat
      361 tatggctgga aatggtagta tgtgggctgc tttgaaagaa aaaatttgcc atttgaaatg
      421 tagagataat ttcaggttac ttggtgaaat ctatccagct actatagttt accaaatatc
      481 ggatttattg tatattcctt ctgatacaga aggaatgcct atgtgtgttt tagagtcaat
      541 gtcacaagga actccggttt tagctagtaa tgttggcggg ttaagtgaaa ttattgaaca
      601 tagggttgat ggatttttat ttgagaagga agatgttgag ggagtgtgtg cttgtgctaa
      661 ttttttactc aatgattctg agtatttgaa atatataggc gagaatagta aatcaaaaat
      721 aagaaaacat ttttctgtgc aaaaaatgtt tgtagaaacc atgagagtat atgatgaatt
      781 attagagaag agtagtcatg gataggaata tagatcaaga attagtaagt attataatac
      841 caacacataa taggtatgaa tctctgatta gagctgtgaa aagttgttta catcaaagtt
      901 ata
//
LOCUS       DAVKAO010000142          876 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKAO010000142 DAVKAO010000000
VERSION     DAVKAO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.142"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(88..234)
                     /locus_tag="VWX16_002018"
     CDS             complement(88..234)
                     /locus_tag="VWX16_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9159300.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVVDRTDKVSNHTHGKATLTWFEEI
                     FEEY"
     gene            248..609
                     /locus_tag="VWX16_002019"
                     /pseudo
     CDS             248..609
                     /locus_tag="VWX16_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
BASE COUNT          279 a          129 c          169 g          299 t
ORIGIN      
        1 gtacggtggt gtgagagggg ctagagatta tcccctactc gatttcgaaa tctagtggaa
       61 tgaatctgga atagtccatc gagctttcta atactcttcg aaaatctctt caaaccacgt
      121 caacgtcgcc ttgccgtgcg tatggttact gactttgtca gttctatcca caacctcaaa
      181 acagtgtttt gagcaacctg cggctagttt cctagtttgc tctttggttt tcattgagta
      241 taacacattg ttagaagttg gtttaaattc cctaatcagt ttgttcacat ttaccttcga
      301 tatattatat cccatagtta aggttggtca tacagatgat tatagtcatg gagccgtaaa
      361 acttagtgtt tctttagttg acaaagatgc catgaaaaaa tatctgtaac tgtaatagga
      421 tattttgaaa taaatataga tgaaaatatc accgatattc tatacgtaaa tggtactgct
      481 attctttgtc cttatttacg ttctattgtt tcaatagttt cggcaattga tagcagtgaa
      541 gcaatgttgc tacctaccat taatgtttta gagttactag ataaatctca atcttttgaa
      601 gaagaataat ttattagctc actaaattga gggtaaggaa aagtaaaagc agtaagaaaa
      661 atgtcttgca ttatacagca accttttggg aatgagtaga tggattgaat aaaatttgat
      721 taagagtgga tgatttatct gtagattatt attggacagt tagtcttgaa gtagtctaag
      781 aattaggtta taatcagtag aagccttgct aataatgagg aggttagttt atgtatagta
      841 gactgaatct aaaatagtac gaaacaattg ctaaaa
//
LOCUS       DAVKAO010000143          772 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKAO010000143 DAVKAO010000000
VERSION     DAVKAO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.143"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            291..590
                     /locus_tag="VWX16_002020"
     CDS             291..590
                     /locus_tag="VWX16_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9159301.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          290 a          143 c          131 g          208 t
ORIGIN      
        1 agatagaggt aacatgtttg caaacaaagc atacgaacct ttagtaaaat catttccatg
       61 aaactagaat agagcccctc ttagcaaaaa tcattatttt aatttatttc taatcactcc
      121 ttgacataaa taactctcac caataaaaga ctatgtctta aaaaaatggt ataataaaat
      181 caatacttgg gcttgatggc tatgctacta ataacaatta ggagagaaaa tcaggcactt
      241 gttaacaaca aggattatcc ccttgagatg aaaggaactt tagaaatctt atgatgaaca
      301 tgcaaaacat gatgcgtcaa gcacaaaaac ttcaaaaaca aatggaacaa agccaagctg
      361 aacttgctgc tatgcaattt gttggcaaat ctgctcaaga tcttgtccaa gcaaccttaa
      421 ctggcgataa gaaagttgtc agcattgatt tcaatccagc tgtcgttgac ccagaagacc
      481 ttgagactct ttctgatatg accgttcaag ccatcaactc tgctcttgaa caaatcgatg
      541 aaactactaa gaaaaaactg ggtgctttcg ctgggaaatt gccattctaa aaacaaggag
      601 ctagaacaat gcttgtcgat aacaaaggct aagaaaggtg caaaaattat aaaaaacgca
      661 tagtatcaag caacctaaaa ctttgggctc tataatattt gtagtgggta aatcccctat
      721 ggatattatg gagcctattt ttgtgtagaa aaaaagtccc atatgaccta ta
//
LOCUS       DAVKAO010000144          721 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKAO010000144 DAVKAO010000000
VERSION     DAVKAO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.144"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            1..641
                     /locus_tag="VWX16_002021"
                     /pseudo
     CDS             1..641
                     /locus_tag="VWX16_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          227 a          160 c          122 g          212 t
ORIGIN      
        1 tactagacat taaagaccca aacatcaaga ttctagatat catcaataag gatacacaca
       61 aagaaatcat cgccaaactg gactacgacg cctcatcttg ccctgagtgc ggaagtcaaa
      121 tgaagaaata tgactttcaa aaaccgtcta agattcctta ccttgagaca actggtatgc
      181 ttactagaat tctccttaga aaacgccgtt tcaagtgcta tcactgttca aaaatgatgg
      241 ttgctgagac tcccctggta aagaaaaatc accaaatccc tcgtatcatc aaccaaaaaa
      301 ttgctcaaaa gttaattgaa aaaatttcta tgactgatat tgcccatcag ctggccattt
      361 caacttcaac tgttattcgc aagctcaatg actttcactt tgagcatgat ttttctcggc
      421 ttccaaagat tatgtcttgg gatgagtatg ccttcactaa gggaaagatg agtttcattg
      481 cgcaagattt tgataatctt aatattatca ctgttcttga aggcagaaca caagctgtca
      541 tccgaaatca ctttcttcgc tacgatagag ccgttcgttg tcaagtgaaa atcattacga
      601 tggatatgtt tagtccttac tatgacttgg ctaaacagct tcgctttcaa atttctaggc
      661 tcaggctgaa acagtctccc agactgtttc actcccgaat gctaaaatcg ttcttgatcg
      721 c
//
LOCUS       DAVKAO010000145          565 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKAO010000145 DAVKAO010000000
VERSION     DAVKAO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.145"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(1..487)
                     /locus_tag="VWX16_002022"
                     /pseudo
     CDS             complement(1..487)
                     /locus_tag="VWX16_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452046.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          181 a          113 c           61 g          210 t
ORIGIN      
        1 gaacgattta gataaatatc tttaagcgtc tcttttttag tttcaaaatc aactacttta
       61 atagagtttt cgttaaaaaa tttaaaaagc tcactacttt gaatattccc agacatagta
      121 attctcagtc cctcttcctg aacaatatcc ccaaattctt gtttggtaat gaaaatgtct
      181 ctatctgtag ctgatgaaaa agctatctca aacaagaaat tatgactatc ttttcctact
      241 ttttgaaatg tcaaaagccc gctctccaag aaaagaaccc tctcacaaat ttcttcaata
      301 cattatcact gcttataata ttacctgaag tcggttgatt attctgaaca agaattttca
      361 tcaacgtact cttaccaact ccatttctcc ctacttgacc aacaatttca cccttgttaa
      421 gtgcaaaatt tatatctttg agaatgattg aatgaccata atgcttactt acatttataa
      481 cttacatttt ttattcctca attaatattc atggtttgct ctttgattct tattgagttt
      541 aaaaagttgc gcctcttttt aacaa
//
LOCUS       DAVKAO010000146          549 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKAO010000146 DAVKAO010000000
VERSION     DAVKAO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.146"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            132..506
                     /locus_tag="VWX16_002023"
     CDS             132..506
                     /locus_tag="VWX16_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9159302.1"
                     /translation="MELVLPNNYVALEQEEMMYFDGGFSIPRWPVATAINIAFNDVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          187 a           74 c          122 g          166 t
ORIGIN      
        1 taggtaacct ataaaaacaa ataaggtaga gaaaggatat cttatttaga taactaagta
       61 ctataaacac agtcgacaat cacttgttag gaaaccatcc taactatgct taataaaaaa
      121 ggagaaaata aatggaactc gtattaccaa ataattatgt tgctcttgag caagaagaga
      181 tgatgtattt tgatggggga ttttctattc cgagatggcc tgttgcaaca gccattaata
      241 tagcttttaa tgatgtctta ggtggaggag caatcagtct agttagaaat tatattcgta
      301 attatggttt gcggcgagtt acaagcgcaa ttgctggagc agctgcaaga tatgttgggg
      361 tacgagttgc aaatagagtg gcaggatttg cactgtctgc tattaatgga tttgcagctt
      421 ggatgtcaat tggcgatgct attacaacaa tctgggccaa caatgatgta aataggagag
      481 acccaaattt aaacgccttg tggtaactag atatgggtat taaaatggaa aaaatttttg
      541 ttattattt
//
LOCUS       DAVKAO010000147          491 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKAO010000147 DAVKAO010000000
VERSION     DAVKAO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.147"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
     gene            complement(<1..>491)
                     /locus_tag="VWX16_002024"
     CDS             complement(<1..>491)
                     /locus_tag="VWX16_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9159303.1"
                     /translation="RTEIMQTYLNMTSIIGSNSLVHFFETCQDYLKTHHDLPIEEIRD
                     MLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLKILNTILFSFPIEYLH
                     LITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTLLEDAKNKK
                     SYD"
BASE COUNT          129 a           76 c          106 g          180 t
ORIGIN      
        1 ctatcgtagc ttttcttatt cttggcgtcc tccagtaaag tgtagcagat ttgcttacac
       61 atgtttttat cttgattgta tagatagagt gtggaaaggt tgagcaggat tgctattcgc
      121 aagtcatata aatgttggta gtttttatag acttctaggc gttgcaatat ttttccagtg
      181 atgagatgga ggtattcaat gggaaaactg aaaagaatgg tattgaggat ttttagatca
      241 ctctcatacc atgtatcttg tttttcaatt tttctccaaa gttttttgac aacctgttct
      301 gcgtgatctg atagttcccc tgccccgtgt tgacggatat aaatgacaac ttcaagcata
      361 tctctgattt cttctatagg gagatcgtgg tgggtcttga ggtagtcttg gcatgtttca
      421 aaaaaatgaa ctagactatt actaccaatg atggaagtca tattgagata agtttgcatg
      481 atttctgttc g
//
LOCUS       DAVKAO010000148          478 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073882
            SAMN33606153-rid17954553.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKAO010000148 DAVKAO010000000
VERSION     DAVKAO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606153
            Sequence Read Archive: SRR23727659
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722701.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722701.1
            Genome Coverage       :: 361x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 01:57:53
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,024
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,851
            CDSs (with protein)               :: 1,851
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 67 of 127
            Pseudo Genes (incomplete)         :: 58 of 127
            Pseudo Genes (internal stop)      :: 33 of 127
            Pseudo Genes (multiple problems)  :: 28 of 127
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606153-rid17954553.denovo.148"
                     /strain="AUSMDU00073882"
                     /serotype="3"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST180"
BASE COUNT          172 a           74 c           81 g          151 t
ORIGIN      
        1 caggtttccg tcattttgaa acctatattg aagatttgca tgatgccaaa ggcaataaaa
       61 tcgaccgcgc tccaaatcca atggaactat tgactattaa agtcccataa cctgttcaat
      121 caggagacat ggttcgtgca ttaaaagaag gactcatcaa tctttataag gaagatggaa
      181 ccagcgtcac agttcgtgct taatgtagtt gtttagtttt aaaaaattat gcaaagctcc
      241 atatacaaca cttaaacgag attaaagaat ggcgaaatcc cttgatgcgc aagagattag
      301 ctgtcctttt ttatttttta agtgataaag tcggagctta ggtatcaaag cctatcaaat
      361 taaacaaaga agcgatgtct tagatatttt gaaaaaaatt aataagcaaa aaactctcta
      421 ttattttgtt gtagagagtt ttttgttaat aaaattttac aaaatgacat ttatatat
//
